title: Molecular characterisation of the tammar wallaby (Macropus eugenii) CD3 epsilon chain cDNA.
abstract: The cDNA encoding the epsilon chain of the tammar wallaby CD3 complex (CD3epsilon) was isolated by PCR. This is the first CD3 component to be cloned in a marsupial. The tammar wallaby cDNA coding region was 61.7 and 63.0% identical to the human and mouse cDNA coding sequences, respectively. Similarly, the predicted amino acid sequence was 56.5 and 52.9% identical to the human and mouse sequences. When compared with other known CD3epsilon peptide sequences, the most conserved region of the tammar wallaby CD3epsilon chain peptide was the cytoplasmic domain and the least conserved was the extracellular portion. Phylogenetic reconstruction based on the deduced amino acid sequence placed the tammar wallaby sequence in its expected position outside of all the eutherian mammals.
authors.affiliation: Cooperative Research Centre for the Conservation and Management of Marsupials, Sydney, Australia.
authors.forename: J M
authors.initials: JM
authors.lastname: Old
authors.forename: E M
authors.initials: EM
authors.lastname: Deane
authors.forename: G A
authors.initials: GA
authors.lastname: Harrison
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017252:CD3 Complex; D017124:Conserved Sequence; D018076:DNA, Complementary; D006801:Humans; D007614:Macropodidae / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
chemical_list: D017252:CD3 Complex; C441388:CD3 epsilon, wallaby; C115335:CD3E protein, human; D018076:DNA, Complementary; D011948:Receptors, Antigen, T-Cell