title: Studies on interaction of phosphorylase kinase from rabbit skeletal muscle with glycogen in the presence of ATP and ADP.
abstract: The influence of ATP on complex formation of phosphorylase kinase (PhK) with glycogen in the presence of Ca(2+) and Mg(2+) has been studied. The initial rate of complex formation decreases with increasing ATP concentration, the dependence of the initial rate on the concentration of ATP having a cooperative character. Formation of the complex of PhK with glycogen in the presence of ATP occurs after a lag period, which increases with increasing ATP concentration. The dependence of the initial rate of complex formation (v) on the concentration of non-hydrolyzed ATP analogue, beta,gamma-methylene-ATP, follows the hyperbolic law. A correlation between PhK-glycogen complex formation and (32)P incorporation catalyzed by PhK itself and by the catalytic subunit of cAMP-dependent protein kinase has been shown. For ADP (the product and allosteric effector of the PhK reaction) the dependence of v on ADP concentration has a complicated form, probably due to the sequential binding of ADP at two allosteric sites on the beta subunit and the active site on the gamma subunit.
authors.affiliation: A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky prospect, Moscow, Russia.
authors.forename: I E
authors.initials: IE
authors.lastname: Andreeva
authors.forename: V F
authors.initials: VF
authors.lastname: Makeeva
authors.forename: N B
authors.initials: NB
authors.lastname: Livanova
authors.forename: S P
authors.initials: SP
authors.lastname: Petukhov
authors.forename: B I
authors.initials: BI
authors.lastname: Kurganov
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004789:Enzyme Activation; D006003:Glycogen / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D009391:Nephelometry and Turbidimetry; D010764:Phosphorylase Kinase / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006003:Glycogen; D010764:Phosphorylase Kinase; D008274:Magnesium; D002118:Calcium