title: Purification of plasmids for gene therapy and DNA vaccination.
abstract: This chapter covers the different aspects of the production and purification of plasmids for gene therapy and DNA vaccination. Process issues are extensively covered and complemented with information related to plasmid DNA structure, vector construction, product specifications and quality assurance and control.
authors.affiliation: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. prazeres@alfa.ist.utl.pt
authors.forename: D M
authors.initials: DM
authors.lastname: Prazeres
authors.forename: G A
authors.initials: GA
authors.lastname: Monteiro
authors.forename: G N
authors.initials: GN
authors.lastname: Ferreira
authors.forename: M M
authors.initials: MM
authors.lastname: Diogo
authors.forename: S C
authors.initials: SC
authors.lastname: Ribeiro
authors.forename: J M
authors.initials: JM
authors.lastname: Cabral
mesh_terms: D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004926:Escherichia coli / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods / Q000592:standards; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics / Q000302:isolation & purification* / Q000592:standards; D011786:Quality Control; D019444:Vaccines, DNA / Q000235:genetics / Q000302:isolation & purification* / Q000592:standards
chemical_list: D019444:Vaccines, DNA