title: Development of a solid-phase binding assay and identification of nonpeptide ligands for the FynB Src homology 2 domain.
abstract: The nonreceptor tyrosine kinase FynB is known to be required in the induction of long-term potentiation (LTP), a cellular mechanism for learning and memory. Ligands of the FynB SH2 domain as a possible FynB activator are, thus, of great interest. In this study, a solid-phase ligand binding assay was established to meet the screening requirement of high-throughput and ease of use, and in an attempt to find the specific ligands for the FynB SH2 domain. This assay measures the competitive inhibition of the binding of the biotinylated phosphopeptide (GGSETDDY*AEIID), derived from a binding sequence in human focal adhesion kinase, to the SH2 domain of FynB precoated as a glutathione S-transferase fusion protein on a solid-phase. Using this high-throughput screening method for SH2 ligands, a modest size of chemical library was screened, and two non-peptide compounds, 4-acetamidobenzene sulfinic acid and 1-allylpyridinium 3-sulfonate, were identified by their strong binding affinity to the FynB SH2 domain. This result demonstrates the feasibility of the developed assay in high-throughput screening. Further studies on the molecular structures of the identified SH2-binding ligands will allow presentation of specific models for ligand-domain complexes for improving the ligands and will help to develop a potential lead compound for improving LTP.
authors.affiliation: Jinis Biopharmaceuticals, San 2-20, KumAm-Dong, Chonju, 561-182, Chonbuk, South Korea.
authors.forename: Hyeon-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hwang-Ho
authors.initials: HH
authors.lastname: Lee
authors.forename: Hye-Dong
authors.initials: HD
authors.lastname: Yoo
authors.forename: John
authors.initials: J
authors.lastname: Hwa Lee
authors.forename: Seong-Tshool
authors.initials: ST
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D001667:Binding, Competitive; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000737:chemistry*; D005982:Glutathione Transferase / Q000737:chemistry; D008024:Ligands; D008722:Methods; D010750:Phosphoproteins / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D018909:src Homology Domains
chemical_list: D002352:Carrier Proteins; D008024:Ligands; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase