Allopurinol has immunomodulating activity following topical and systemic application in experimental autoimmune uveitis.
OBJECTIVE
Allopurinol has beneficial effects in the systemic treatment of lens-induced uveitis and experimental autoimmune uveitis (EAU). This is believed to be due to a reduction of oxidative tissue damage through its dose-dependent free radical scavenging ability, and to an immunomodulating effect. The purpose of this study was to demonstrate the immunological effects on the IgG-antibody repertoire in EAU after topical and systemic allopurinol and steroids.


METHODS
We assigned 43 male Lewis rats to 6 different groups: healthy rats (BASE, n=3)
Grus
F H
FH
Department of Ophthalmology, University of Mainz, Germany. Fgrus@CSI.COM
Augustin
A J
AJ
Loeffler
K
K
Dick
B
B
Lutz
J
J
Pfeiffer
N
N
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000627:therapeutic use*; D000287:Administration, Topical; D000493:Allopurinol / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D015153:Blotting, Western; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000627:therapeutic use*; D005938:Glucocorticoids / Q000008:administration & dosage / Q000627:therapeutic use; D007074:Immunoglobulin G / Q000097:blood; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014605:Uveitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D001324:Autoantigens; D016166:Free Radical Scavengers; D005938:Glucocorticoids; D007074:Immunoglobulin G; D000493:Allopurinol