title: Three-dimensional structure of a new form of mare lactoferrin in 70% PEG 400 at 3.8 A resolution.
abstract: Three-dimensional (3D) structure of a new form of diferric mare lactoferrin has been determined at 3.8 A resolution. The protein was crystallized in a space group P2(1)2(1)2(1) with a = 80.1 A, b = 103.7 A, c = 112.2 A with a solvent content of 57%. The structure was solved by molecular replacement method using the model of native mare lactoferrin. The structure has been refined using X-PLOR to a final R-factor of 22.6% for all the data in 15.0-3.8 A resolution range. The final refined model comprises 5281 protein atoms, 2Fe3+ and 2CO3(2-) ions. The protein folds into two globular N- and C-lobes. The two lobes are further divided into two domains N1, N2 in the N-lobe and C1, C2 in the C-lobe. The overall folding of the protein is similar to that observed for the native protein. The superposition of Calpha traces of native mare lactoferrin and the present structure gives an r.m.s shift of 0.7 A. There is a slight variation in the orientation of two lobes but the domain orientations in the present structure are identical to those observed in the native mare lactoferrin.
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: M
authors.initials: M
authors.lastname: Paramasivam
authors.forename: A
authors.initials: A
authors.lastname: Srinivasan
authors.forename: T P
authors.initials: TP
authors.lastname: Singh
mesh_terms: D000818:Animals; D000838:Anions; D001665:Binding Sites; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006736:Horses; D007781:Lactoferrin / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D000838:Anions; D011092:Polyethylene Glycols; D007781:Lactoferrin