Vaccination with attenuated simian immunodeficiency virus by DNA inoculation.
Delivering attenuated lentivirus vaccines as proviral DNA would be simple and inexpensive. Inoculation of macaques with wild-type simian immunodeficiency virus strain mac239 (SIV(mac239)) DNA or SIV(mac239) DNA containing a single deletion in the 3' nef-long terminal repeat overlap region (nef/LTR) led to sustained SIV infections and AIDS. Injection of SIV(mac239) DNA containing identical deletions in both the 5' LTR and 3' nef/LTR resulted in attenuated SIV infections and substantial protection against subsequent mucosal SIV(mac251) challenge.
Kent
S J
SJ
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia. skent@unimelb.edu.au
Dale
C J
CJ
Preiss
S
S
Mills
J
J
Campagna
D
D
Purcell
D F
DF
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D018791:CD4 Lymphocyte Count; D017931:DNA Primers; D007371:Interferon-gamma / Q000096:biosynthesis; D008254:Macaca nemestrina; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D012367:RNA, Viral / Q000097:blood; D012091:Repetitive Sequences, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
D000914:Antibodies, Viral; D017931:DNA Primers; D012367:RNA, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines; D007371:Interferon-gamma