Ocular toxicity study of trypan blue injected into the vitreous cavity of rabbit eyes.
BACKGROUND
To evaluate the ocular toxicity of trypan blue (TB) injected into the vitreous cavity of rabbit eyes. TB is a dye that could be useful for staining epiretinal membranes during vitrectomy surgery.


METHODS
Ten New Zealand White (NZW) rabbits underwent gas-compression vitrectomy. Rabbits were divided into three groups to receive injections of 0.1 ml basic salt solution, 0.1 ml of a 0.06% TB solution or 0.1 ml of a 0.2% TB solution. Ocular toxicity was assessed by slit-lamp biomicroscopy, ophthalmoscopy, electroretinography and histology.


RESULTS
Transient posterior capsule opacification was noted in all animals. No significant reductions in a-wave or b-wave amplitudes were found in any of the animals. Light and electron microscopic examination of the inferior retina in the 0.2% TB-treated eyes showed damaged photoreceptors and marked disorganization. Immunohistochemical staining for rhodopsin was strongly reduced in those sections and staining for proliferation with Ki-67 was positive. No histological abnormalities were found in the upper retina of the 0.2% TB-treated eyes or in any part of the retina of the 0.06% TB-treated or control eyes. No histological abnormalities were found in any of the anterior chamber angle specimens.


CONCLUSIONS
Although no signs of toxicity were found after the prolonged presence of TB at a concentration of 0.06% in the vitreous cavity of rabbit eyes, marked damage occurred in the lower retina of 0.2% TB-treated eyes. The short-term presence of TB at a concentration of 0.06% in the vitreous cavity is harmless to the rabbit eye but a higher concentration of TB could be unsafe.
Veckeneer
M
M
Rotterdam Eye Hospital, The Netherlands. veckeneer@oogziekenhuis.nl
van Overdam
K
K
Monzer
J
J
Kobuch
K
K
van Marle
W
W
Spekreijse
H
H
van Meurs
J
J
D000818:Animals; D002386:Cataract / Q000139:chemically induced*; D004396:Coloring Agents / Q000633:toxicity*; D004596:Electroretinography; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D007903:Lens Capsule, Crystalline / Q000187:drug effects*; D009883:Ophthalmic Solutions; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012162:Retinal Degeneration / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D012243:Rhodopsin / Q000378:metabolism; D014343:Trypan Blue / Q000633:toxicity*; D014821:Vitrectomy; D014822:Vitreous Body / Q000187:drug effects*
D004396:Coloring Agents; D019394:Ki-67 Antigen; D009883:Ophthalmic Solutions; D012243:Rhodopsin; D014343:Trypan Blue