title: Presence of prokaryotic and eukaryotic species in all subgroups of the PP(i)-dependent group II phosphofructokinase protein family.
abstract: Inorganic pyrophosphate-dependent phosphofructokinase (PP(i)-PFK) of the amitochondriate eukaryote Mastigamoeba balamuthi was sequenced and showed about 60% identity to PP(i)-PFKs from two eubacteria, Propionibacterium freudenreichii and Sinorhizobium meliloti. These gene products represent a newly recognized lineage of PFKs. All four lineages of group II PFKs, as defined by phylogenetic analysis, contained both prokaryotic and eukaryotic species, underlining the complex evolutionary history of this enzyme.
authors.affiliation: The Rockefeller University, New York, New York 10021, USA. mmuller@mail.rockeffer.edu
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: J A
authors.initials: JA
authors.lastname: Lee
authors.forename: P
authors.initials: P
authors.lastname: Gordon
authors.forename: T
authors.initials: T
authors.lastname: Gaasterland
authors.forename: C W
authors.initials: CW
authors.lastname: Sensen
mesh_terms: D000656:Amoeba / Q000201:enzymology; D000818:Animals; D005057:Eukaryotic Cells / Q000201:enzymology*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D011387:Prokaryotic Cells / Q000201:enzymology*
chemical_list: D010770:Phosphotransferases; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase