Evaluation in vitro of canine neutrophil function.
Polymorphonuclear granulocyte (PMN) phagocytosis may be affected by many pathological changes. A panel of tests requiring relatively small volumes of blood was applied to 16 healthy dogs in order to obtain normal values and to standardize techniques. PMNs were isolated by discontinuous Percoll gradients; chemotaxis was tested in a modified Boyden chamber using the leading front method; fluorescinated yeast uptake was evaluated on a slide and superoxide (SO) production and adherence was carried out on a microtitre plate. The different aspects of phagocytosis showed no correlation with one another. Better results were obtained using a 60 min incubation period using interleukin-8 (25 ng/mL) as an activator for chemotaxis, and incubating plates for 30 min with phorbol myristate acetate (10(-6)mol/L) to assess SO production.
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Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione di Patologia Generale Veterinaria e Parassitologia, via Celoria 10, 20133 Milano, Italy. stex@mailserver.unimi.it
Paltrinieri
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Spagnolo
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Sartorelli
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D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002633:Chemotaxis; D004285:Dogs / Q000502:physiology*; D005260:Female; D016209:Interleukin-8 / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D012016:Reference Values; D013481:Superoxides / Q000032:analysis
D016209:Interleukin-8; D013481:Superoxides