title: Immunohistochemical expression of G protein alpha-subunit isoforms in rat and monkey Merkel cell-neurite complexes.
abstract: The true function of Merkel cells (MCs) is still enigmatic, though the localization of various kinds of neurotransmitter-like substances in MCs has been revealed by immunohistochemistry. Most of the neurotransmitters act on target cells via seven-transmembrane receptors coupled to heterotrimeric G proteins. The heterotrimeric G proteins include various subfamilies that contribute to different signal transduction pathways. Therefore investigation of specific types of G proteins in MCs and related axon terminals (MC-axon terminals) should contribute to the elucidation of the function of MCs. In this study, we investigated the expression patterns of alpha-subunit isoforms of G proteins in MC-neurite complexes of the rat and monkey by enzymatic and fluorescence immunohistochemistry. MC-axon terminals of the rat and monkey showed positive immunoreactions of Galphao and Galphai1. Those of the monkey also showed a weak immunoreaction of Galphas. On the other hand, MCs of both animals showed positive immunoreactions of Galphao, Galphai1, Galphaq, and Galphaz. In addition, MCs of the monkey showed weak immunoreactions of Galphas. Galphao- and Galphai1-like immunoreactions in the MC-axon terminals suggest that MCs suppressively regulate receptive functions of type I mechanosensory nerve terminals. On the other hand, the localization of Galpha-subunits in MCs suggests that these cells are regulated with hormones, neurotransmitter-like substances, or growth factors.
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Iwate Medical University, Morioka 020-8505, Japan. tatamiko@iwate-med.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Tachibana
authors.forename: M
authors.initials: M
authors.lastname: Endoh
authors.forename: T
authors.initials: T
authors.lastname: Nawa
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry; D020962:Heterotrimeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000378:metabolism; D007150:Immunohistochemistry; D008251:Macaca; D018862:Merkel Cells / Q000737:chemistry*; D018613:Microscopy, Confocal; D016501:Neurites / Q000737:chemistry*; D051381:Rats; D012867:Skin / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D020962:Heterotrimeric GTP-Binding Proteins