title: In vitro susceptibilities of Bartonella and Rickettsia spp. to fluoroquinolone antibiotics as determined by immunofluorescent antibody analysis of infected Vero cell monolayers.
abstract: The in vitro susceptibilities of Bartonella and Rickettsia spp. to different concentrations of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin in Vero cell cultures, were determined by enumeration of immunofluorescent-stained bacilli. After incubation in a CO(2)-enriched atmosphere, inocula were replaced and tested with media containing 12 different concentrations of each antibiotic in replicate for each species and the monolayers were re-incubated. Growth status was determined by evaluation of immunofluorescent staining bacilli. Effective inhibitory antibiotic dilution endpoints were determined by counting Bartonella- and Rickettsia-specific fluorescent foci across a range of antibiotic dilutions with an epi-fluorescent microscope, and were compared with an antibiotic-negative control. Based upon the use of C(max):MIC and AUC:MIC data, levofloxacin exhibited activity against Bartonella elizabethae and B. quintana.
authors.affiliation: School of Pharmacy, Campus Box 7595, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7595, USA. tjives@med.unc.edu
authors.forename: T J
authors.initials: TJ
authors.lastname: Ives
authors.forename: E L
authors.initials: EL
authors.lastname: Marston
authors.forename: R L
authors.initials: RL
authors.lastname: Regnery
authors.forename: J D
authors.initials: JD
authors.lastname: Butts
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001473:Bartonella / Q000187:drug effects*; D002522:Chlorocebus aethiops; D015169:Colony Count, Microbial; D005455:Fluorescent Antibody Technique; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D012281:Rickettsia / Q000187:drug effects*; D014709:Vero Cells / Q000382:microbiology
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones