title: Assembly of microarrays for genome-wide measurement of DNA copy number.
abstract: We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.
authors.affiliation: Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Snijders
authors.forename: N
authors.initials: N
authors.lastname: Nowak
authors.forename: R
authors.initials: R
authors.lastname: Segraves
authors.forename: S
authors.initials: S
authors.lastname: Blackwood
authors.forename: N
authors.initials: N
authors.lastname: Brown
authors.forename: J
authors.initials: J
authors.lastname: Conroy
authors.forename: G
authors.initials: G
authors.lastname: Hamilton
authors.forename: A K
authors.initials: AK
authors.lastname: Hindle
authors.forename: B
authors.initials: B
authors.lastname: Huey
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Law
authors.forename: K
authors.initials: K
authors.lastname: Myambo
authors.forename: J
authors.initials: J
authors.lastname: Palmer
authors.forename: B
authors.initials: B
authors.lastname: Ylstra
authors.forename: J P
authors.initials: JP
authors.lastname: Yue
authors.forename: J W
authors.initials: JW
authors.lastname: Gray
authors.forename: A N
authors.initials: AN
authors.lastname: Jain
authors.forename: D
authors.initials: D
authors.lastname: Pinkel
authors.forename: D G
authors.initials: DG
authors.lastname: Albertson
mesh_terms: D000782:Aneuploidy; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D018628:Gene Dosage; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D014407:Tumor Cells, Cultured; D014960:X Chromosome / Q000235:genetics