title: Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein.
abstract: Adenosine production catalysed by cytosolic 5'-nucleotidase (cN-I) regulates diverse physiological processes. We report here a mouse cN-I (mcN-I) cloned from heart and testis. The open reading frame contains several potential translation initiation sites, which yield similarly active 5'-nucleotidases. Using overexpression in COS-7 cells we showed that mcN-I, like the previously cloned pigeon cN-I, is activated by ADP and catalyses adenosine formation during ATP breakdown. The N- and C-termini of mcN-I and pcN-I are divergent. Deletion of the 12 C-terminal amino acids or the first 19 N-terminal amino acids of pcN-I does not diminish activity, although deletion of the first 31 N-terminal amino acids reduces activity by 70%. Overall mcN-I is only 66% identical to pcN-I or the recently cloned human cN-I (hcN-I), while hcN-I and pcN-I are 85% identical. We report here a partial hcN-I sequence that is only 70% identical with the published hcN-I amino acid sequence but is 87% identical with mcN-I. Both hcN-I sequences have perfect matches to distinct human genome sequences. Our data imply the existence of at least two genes for cN-I, cN-I(A), previously cloned from pigeon and human, and cN-I(B) that we report here from mouse and partially from human.
authors.affiliation: Bristol Heart Institute, Bristol Royal Infirmary, University of Bristol, BS2 8HW, Bristol, UK. g.newby@bris.ac.uk
authors.forename: G B
authors.initials: GB
authors.lastname: Sala-Newby
authors.forename: A C
authors.initials: AC
authors.lastname: Newby
mesh_terms: D015720:5'-Nucleotidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003600:Cytosol / Q000201:enzymology*; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009705:Nucleosides / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013737:Testis / Q000201:enzymology
chemical_list: D009705:Nucleosides; D012333:RNA, Messenger; D010770:Phosphotransferases; D015720:5'-Nucleotidase