Characterization of a cDNA encoding the bovine coxsackie and adenovirus receptor.
Non-human adenoviruses such as bovine adenovirus type 3 (BAV-3) that do not replicate in human cells but can infect human cells in culture could provide an attractive alternative to human adenoviral vectors for gene therapy. In addition, a large-animal model for genetic diseases can be very useful for the assessment of the efficacy of adenovector-mediated gene delivery in man. Recombinant human subgroup C adenovectors use the coxsackie and adenovirus receptor (CAR) to enter their target cells. Through RT-PCR and sequencing we determined the complete coding sequence of bovine CAR which serves as the primary adenoviral attachment site on bovine cells. A multiple sequence alignment, involving all the previously identified CAR species (man, mouse, rat, pig, and dog) showed that bovine CAR was most related to porcine CAR (92% nucleotide similarity) and demonstrated a highly conserved adenovirus binding Ig1 domain.
Thoelen
I
I
Laboratory of Clinical and Epidemiological Virology, University of Leuven, Belgium.
Keyaerts
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E
Lindberg
M
M
Van Ranst
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D017124:Conserved Sequence; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D018076:DNA, Complementary / Q000235:genetics; D015316:Genetic Therapy / Q000662:veterinary; D006801:Humans; D017921:Mastadenovirus / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017385:Sequence Homology
C568771:CLMP protein, human; C568772:CLMP protein, mouse; C568773:Clmp protein, rat; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D018076:DNA, Complementary; D012333:RNA, Messenger; D011991:Receptors, Virus