title: Three-dimensional structure of a purple lipoxygenase.
abstract: Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 A resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity.
authors.affiliation: Department of Chemistry, University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606, USA. ejankun@protein.wo.utoledo.edu
authors.forename: E
authors.initials: E
authors.lastname: Skrzypczak-Jankun
authors.forename: R A
authors.initials: RA
authors.lastname: Bross
authors.forename: R T
authors.initials: RT
authors.lastname: Carroll
authors.forename: W R
authors.initials: WR
authors.lastname: Dunham
authors.forename: M O
authors.initials: MO
authors.lastname: Funk
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004578:Electron Spin Resonance Spectroscopy; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D008041:Linoleic Acids / Q000737:chemistry / Q000378:metabolism; D008054:Lipid Peroxides / Q000737:chemistry / Q000378:metabolism; D008084:Lipoxygenase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010545:Peroxides / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D013025:Glycine max / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005290:Ferric Compounds; D008041:Linoleic Acids; D008054:Lipid Peroxides; D010545:Peroxides; D010940:Plant Proteins; C038649:13-hydroperoxy-9,11-octadecadienoic acid; C095864:lipoxygenase 3; D008084:Lipoxygenase