Purification, crystallization and preliminary X-ray diffraction studies of disintegrin (schistatin) from saw-scaled viper (Echis carinatus).
This is the first report of crystallographic data on a disintegrin molecule from any source. The heterodimeric disintegrin with a molecular weight of 14 kDa from Echis carinatus venom is a potent antagonist of alpha4 integrins. The intact disintegrin, containing two subunits A and B, was isolated and purified using affinity and ion-exchange columns. It has been crystallized using 1.6 M ammonium sulfate as a precipitating agent. The crystals grew to dimensions of 0.25 x 0.20 x 0.20 mm and diffracted to 2.5 A resolution. The crystals belong to space group I4(1)22, with unit-cell parameters a = b = 91.7, c = 55.1 A. Assuming a molecular weight of 14 kDa, a V(M) of 2.1 A(3) Da(-1) is obtained for one molecule of disintegrin in the asymmetric unit.
Tomar
S
S
Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India.
Yadav
S
S
Chandra
V
V
Kumar
P
P
Singh
T P
TP
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D019483:Disintegrins / Q000737:chemistry*; D011487:Protein Conformation; D014757:Viper Venoms / Q000737:chemistry*; D017819:Viperidae
D019483:Disintegrins; D014757:Viper Venoms