title: Comprehensive analysis of a large genomic sequence at the putative B-cell chronic lymphocytic leukaemia (B-CLL) tumour suppresser gene locus.
abstract: In many haematological diseases, and more particularly in B-cell chronic lymphocytic leukaemia (B-CLL), the existence of a tumour suppressor gene located within the frequently deleted region 13q14.3, has been put forward. A wide candidate region spanning from marker D13S273 to D13S25 has been proposed and an extensive physical map has been constructed by several teams. In this study, we sequenced a minimal core deleted region that we have previously defined and annotated it with flanking available public sequences. Our analysis shows that this region is gene-poor. Furthermore, our work allowed us to identify new alternative transcripts, spanning core regions, of the previously defined candidate genes DLEU1 and DLEU2. Since their putative involvement in B-CLL was controversial, our present study provide support for reconsidering the DLEU1 and DLEU2 genes as B-CLL candidate genes, with a new definition of their organisation and context.
authors.affiliation: INSERM U463, Institut de Biologie de l'Hôtel-Dieu, Centre Hospitalier Universitaire, 44093 Cedex, Nantes, France.
authors.forename: G
authors.initials: G
authors.lastname: Rondeau
authors.forename: I
authors.initials: I
authors.lastname: Moreau
authors.forename: S
authors.initials: S
authors.lastname: Bézieau
authors.forename: J L
authors.initials: JL
authors.lastname: Petit
authors.forename: R
authors.initials: R
authors.lastname: Heilig
authors.forename: S
authors.initials: S
authors.lastname: Fernandez
authors.forename: E
authors.initials: E
authors.lastname: Pennarun
authors.forename: J S
authors.initials: JS
authors.lastname: Myers
authors.forename: M A
authors.initials: MA
authors.lastname: Batzer
authors.forename: J P
authors.initials: JP
authors.lastname: Moisan
authors.forename: M C
authors.initials: MC
authors.lastname: Devilder
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D030561:Databases, Nucleic Acid; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D016147:Genes, Tumor Suppressor; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*