Determination of the animal origin of raw food by species-specific PCR.
Specific PCR, amplifying a fragment of the mitochondrially encoded cytochrome b gene, are developed for discrimination of chicken, turkey, pig, cow and sheep. These PCR tests could be applied for detection and discrimination of animal food ingredients. For origin identification of milk, the bovine PCR is applied on DNA extracted with the DNeasy Tissue kit. A PCR of 30 cycles reached a sensitivity of 720 somatic cells in the PCR reaction corresponding to 29 x 10(4) ells per ml milk. A PCR of 40 cycles could detect 0.72 to 0.144 somatic cells in the PCR reaction corresponding to 29 to 2.9 cells per ml milk.
Herman
B L
BL
Ministry of Small Enterprises, Traders and Agriculture, Belgium, Agricultural Research Centre, Department for Animal Product Quality, Melle. l.herman@clo.fgov.be
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics; D002645:Chickens / Q000235:genetics; D003573:Cytochrome b Group / Q000235:genetics* / Q000302:isolation & purification; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005504:Food Analysis / Q000379:methods; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012756:Sheep / Q000235:genetics; D013045:Species Specificity; D013552:Swine / Q000235:genetics; D014422:Turkeys / Q000235:genetics
D003573:Cytochrome b Group; D004247:DNA