title: Molecular characterization of a distinct begomovirus infecting tobacco in Yunnan, China.
abstract: Virus isolate Y3V, obtained from tobacco showing leaf curl symptoms in Yunnan, China, had particles with the size and morphology typical of geminiviruses. In reactions with a set of monoclonal antibodies raised against begomoviruses, Y3V was readily differentiated from two previously studied Chinese Begomovirus isolates. The complete nucleotide sequence of a DNA-A-like molecule of Y3V was determined; it comprises 2744 nucleotides and has a typical Begomovirus genome organization. When compared with the DNA-A sequences of other begomoviruses, the total DNA-A of Y3V was most closely related to that of Ageratum yellow vein virus (AYVV) (85% sequence identity), but the Y3V intergenic region differed greatly from those of the other sequences (maximum 70% identity). In contrast, the deduced coat protein of Y3V is most like that of Tomato yellow leaf curl Thailand virus-[1] (TYLCTHV-[1]) (92% amino acid sequence identity). The molecular data show that the Yunnan isolate of Tobacco leaf curl virus is a distinct Begomovirus species, for which the name Tobacco leaf curl Yunnan virus (TLCYnV) is proposed.
authors.affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou, China. xzhou@mail.hz.zj.cn
authors.forename: X P
authors.initials: XP
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: Z K
authors.initials: ZK
authors.lastname: Zhang
mesh_terms: D002213:Capsid / Q000235:genetics; D002681:China; D004279:DNA, Viral / Q000032:analysis; D017791:Geminiviridae / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D010947:Plants, Toxic; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology*
chemical_list: D004279:DNA, Viral