title: Long-term tumor growth suppression in mice immunized with naked DNA of the human tumor antigen mucin (MUC1).
abstract: Naked DNA is an attractive tool for vaccination studies. We have studied naked DNA vaccination against the human tumor antigen mucin, encoded by the gene MUC1. C57/BL6 mice were immunized twice, on day 1 and day 10. with plasmid pCI-MUC1, intramuscularly. Five days after the last immunization tumor challenge experiments were performed using the tumor cell line MC38, expressing human MUC1. In 85% of mice immunized with the mucin plasmid tumor growth inhibition was observed, whereas control mice developed tumors. Re-tumor challenge after three months revealed no tumor growth in mice immunized with the mucin plasmid. These encouraging results, showing long-term protection against tumor growth, indicate the potential usefulness of naked DNA vaccination for clinical immunotherapy.
authors.affiliation: Humboldt-University Berlin, Charité Campus Mitte, AG Molecular Gene- and Immunotherapy, Berlin, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Johnen
authors.forename: H
authors.initials: H
authors.lastname: Kulbe
authors.forename: G
authors.initials: G
authors.lastname: Pecher
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000032:analysis / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D014162:Transfection; D014407:Tumor Cells, Cultured; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D018396:Mucin-1; D019444:Vaccines, DNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor