DNA damage by cadmium and arsenic salts assessed by the single cell gel electrophoresis assay.
Human exposure to metals is frequent due to their ubiquity, wide use in industry, and environmental persistence. Direct and indirect genotoxic effects of cadmium (Cd) and arsenic (As) were reported. However, the mechanisms of induction of genetic damage are not well known. The aim of the present work was to evaluate the degree of damage induced by Cd and As salts in a human lung fibroblasts cell line using the single cell gel electrophoresis assay (SCGE). MRC-5 cells were treated with cadmium chloride (CdCl(2)), cadmium sulfate (CdSO(4)), sodium arsenite (NaAsO(2)) and cacodylic acid (C(2)H(7)AsO(2)). A significant dose-dependent increment in the extent of DNA migration as well as in the percentage of cells with tails was observed (P<0.001) after treatment with CdSO(4) and NaAsO(2). Treatment with CdCl(2) induced a relatively low level of DNA strand breaks in comparison with that induced by CdSO(4). The increase migration observed with the three compounds could be originated either by the direct induction of DNA lesions or by the inhibition of excision repair mechanisms. On the other hand, cells treated with C(2)H(7)AsO(2) showed a decrease in the migration length with the three doses employed (P<0.001). The decrease in the rate of DNA migration could be a consequence of the induction of DNA cross-links by organic arsenicals.Cd and As salts induced DNA damage in fibroblast cells, detected as DNA migration in the single cell gel (SCG) assay. The distribution of DNA migration among cells as a function of dose revealed that the majority of exposed cells showed more DNA damage than cells obtained from control cultures. The potential for human exposure to both metals has been increased over the years due to the increment in their use. For this reason, elucidation of carcinogenic mechanisms is very important.
Mourón
S A
SA
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60y 118 s/n, 1900 La Plata, Argentina.
Golijow
C D
CD
Dulout
F N
FN
D001151:Arsenic / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity; D002101:Cacodylic Acid / Q000633:toxicity; D019256:Cadmium Chloride / Q000633:toxicity; D019187:Cadmium Compounds / Q000633:toxicity*; D002460:Cell Line; D020552:Comet Assay; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D017670:Sodium Compounds / Q000633:toxicity; D013431:Sulfates / Q000633:toxicity
D018053:Arsenites; D019187:Cadmium Compounds; D017670:Sodium Compounds; D013431:Sulfates; C017947:sodium arsenite; D004247:DNA; C037123:cadmium sulfate; D002101:Cacodylic Acid; D019256:Cadmium Chloride; D001151:Arsenic