title: Expression, purification and crystallization of Aspergillus nidulans NmrA, a negative regulatory protein involved in nitrogen-metabolite repression.
abstract: The NmrA repressor protein of Aspergillus nidulans was overproduced in Escherichia coli and purified to homogeneity. Gel-exclusion chromatography showed that NmrA was monomeric in solution under the buffer conditions used. The protein was crystallized in three forms, belonging to trigonal, monoclinic and hexagonal space groups. Two of these crystal forms (A and B) diffract to high resolution and thus appear suitable for structure determination. Crystal form A belongs to space group P3((1))21, with unit-cell parameters a = b = 76.8, c = 104.9 A. Crystal form B belongs to space group C2, with unit-cell parameters a = 148.8, b = 64.3, c = 110.2 A, beta = 121.8 degrees.
authors.affiliation: Structural Biology Division, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, England.
authors.forename: C E
authors.initials: CE
authors.lastname: Nichols
authors.forename: S
authors.initials: S
authors.lastname: Cocklin
authors.forename: A
authors.initials: A
authors.lastname: Dodds
authors.forename: J
authors.initials: J
authors.lastname: Ren
authors.forename: H
authors.initials: H
authors.lastname: Lamb
authors.forename: A R
authors.initials: AR
authors.lastname: Hawkins
authors.forename: D K
authors.initials: DK
authors.lastname: Stammers
mesh_terms: D001233:Aspergillus nidulans / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry*; D017672:Nitrogen Compounds; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012097:Repressor Proteins / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D017672:Nitrogen Compounds; C441635:NmrA protein, Aspergillus nidulans; D011994:Recombinant Proteins; D012097:Repressor Proteins