title: Separation of basic drug enantiomers by capillary zone electrophoresis using glucuronyl glucosyl beta-cyclodextrin as a chiral selector.
abstract: Separations of basic drug enantiomers have been investigated using glucuronyl glucosyl beta-cyclodextrin (GUG beta-CD) as a chiral selector in the background electrolyte by capillary zone electrophoresis. The effects of GUG beta-CD concentration and running buffer pH on the migration times and resolution of 16 basic drug enantiomers were precisely examined using a linear polyacrylamide-coated capillary. High resolution of 16 basic drug enantiomers was generally attained with a running buffer pH 2.5 or 3.5 containing 10 mM GUG beta-CD. Next, we compared the chiral resolution abilities of GUG beta-CD with those of beta-CD and maltosyl beta-CD (G2 beta-CD). GUG beta-CD showed higher resolution for basic drug enantiomers tested than beta-CD and G2 beta-CD. This could be due to that hydrogen bonding or ionic interactions of uncharged and charged glucuronyl glucosyl groups of GUG beta-CD with an analyte could stabilize the inclusion complex.
authors.affiliation: Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsunaga
authors.forename: J
authors.initials: J
authors.lastname: Haginaka
mesh_terms: D000180:Acrylic Resins; D000322:Adrenergic Agonists / Q000737:chemistry / Q000302:isolation & purification*; D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D000777:Anesthetics / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D002240:Carbohydrate Sequence; D003505:Cyclodextrins / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000031:analogs & derivatives* / Q000737:chemistry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D013566:Sympathomimetics / Q000737:chemistry / Q000302:isolation & purification*; D047392:beta-Cyclodextrins; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000322:Adrenergic Agonists; D000319:Adrenergic beta-Antagonists; D000777:Anesthetics; D002021:Buffers; D003505:Cyclodextrins; D006634:Histamine H1 Antagonists; D008320:Maltose; D013566:Sympathomimetics; D047392:beta-Cyclodextrins; D000099166:Polyacrylamides; C433303:maltosyl-cyclodextrin; C031215:betadex