[Inhibition of lipid peroxidation in retinal tissue during vitrectomy. Effect of intraocular irrigating solution BSS plus].
OBJECTIVE
Several antioxidant substances have been included in intraocular solutions for vitrectomy in order to reduce oxidative stress. The objective of this work is to evaluate the antioxidant effect of BBS plus(R) (with oxidized glutathione) by means of determining scores of Malondialdehyde (MDA) in retinal tissue at different post vitrectomy time-periods.


METHODS
Thirty pigmented rabbits distributed in three groups (n=10). Group I: Control (no surgery); group II: Vitrectomy adding BSS plus(R), scoring of malondialdehyde at 3 hours post surgery; group III: Vitrectomy adding BBS plus(R), analysis of malondialdehide at 48 hours after vitrectomy. Retinal samples were used to determine the score of malondialdehyde using ion-pairing high performance liquid chromatography. Statistical analysis was performed using ANOVA-Scheffé test (p<0.05).


RESULTS
Scores of free MDA (microgram of retinal tissue) were: group I (n=8): 1.080 S.D. 0.389 microgram group II (n=8): 1.273 S.D. 0.710 microgram and group III (n=10): 0.639 S.D. 0.374 microgram.


CONCLUSIONS
BSS plus(R) showed antioxidant effects in retinal tissue which were observed better at a later rather than early stage after a vitrectomy.
Marcos
M A
MA
Unidad de Vítreo-Retina, Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid. manolo@ioba.med.uva.es
Cordero
Y
Y
Manzanas
L
L
Gómez
E
E
Del Nozal
M J
MJ
Pastor
J C
JC
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001639:Bicarbonates / Q000008:administration & dosage / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D004338:Drug Combinations; D005978:Glutathione / Q000008:administration & dosage / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis; D009883:Ophthalmic Solutions / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D014821:Vitrectomy / Q000009:adverse effects*
D000975:Antioxidants; D001639:Bicarbonates; D015415:Biomarkers; D004338:Drug Combinations; D009883:Ophthalmic Solutions; C013003:glutathione-bicarbonate-Ringer solution; D008315:Malondialdehyde; D005978:Glutathione