Growth-hormone-stimulated dentinogenesis in Lewis dwarf rat molars.
In dentinogenesis, certain growth factors, matrix proteoglycans, and proteins are directly or indirectly dependent on growth hormone. The hypothesis that growth hormone up-regulates the expression of enzymes, sialoproteins, and other extracellular matrix proteins implicated in the formation and mineralization of tooth and bone matrices was tested by the treatment of Lewis dwarf rats with growth hormone over 5 days. The molar teeth were processed for immunohistochemical demonstration of bone-alkaline phosphatase, bone morphogenetic proteins-2 and -4, osteocalcin, osteopontin, bone sialoprotein, and E11 protein. Odontoblasts responded to growth hormone by more cells expressing bone morphogenetic protein, alkaline phosphatase, osteocalcin, and osteopontin. No changes were found in bone sialoprotein or E11 protein expression. Thus, growth hormone may stimulate odontoblasts to express several growth factors and matrix proteins associated with dentin matrix biosynthesis in mature rat molars.
Young
W G
WG
Department of Oral Biology, School of Dentistry, The University of Queensland, Brisbane, Australia.
Li
H
H
Xiao
Y
Y
Waters
M J
MJ
Bartold
P M
PM
D000469:Alkaline Phosphatase / Q000032:analysis / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D001857:Bone Matrix / Q000187:drug effects; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000032:analysis / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D003810:Dentinogenesis / Q000187:drug effects*; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects; D015675:Osteocalcin / Q000032:analysis / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D053495:Osteopontin; D010750:Phosphoproteins / Q000032:analysis / Q000187:drug effects; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D012795:Sialoglycoproteins / Q000032:analysis / Q000187:drug effects; D014074:Tooth Calcification / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000187:drug effects; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; C525720:Bmp2 protein, rat; C525811:Bmp4 protein, rat; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D016326:Extracellular Matrix Proteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; D053495:Osteopontin; D013006:Growth Hormone; D000469:Alkaline Phosphatase