title: Characterization and cloning of two isoforms of heteroglobin, a novel heterodimeric glycoprotein of the secretoglobin-uteroglobin family showing tissue-specific and sex differential expression.
abstract: Heteroglobin (HGB) is a 39-kDa heterodimeric protein detected under non-reducing conditions in harderian, parotid, and submaxillary glands and saliva of the Syrian hamster with antiserum raised against the carboxyl end deduced from the female harderian gland cDNA FHG22 (Dominguez, P. (1995) FEBS Lett. 376, 257-261). After reduction, only one 5.6-kDa polypeptide, named HGB.A, was immunodetected and identified by sequencing as the mature FHG22 product. Tissue-specific expression of HGB.A and HGB mimics that of FHG22 mRNA, with sex differences in submaxillary and harderian glands. Purification of HGB revealed it consists of HGB.A disulfide bonded to HGB.B, a 33.5-kDa N-glycosylated subunit that yields a 9-kDa core polypeptide after deglycosylation. Two highly homologous (96.2%) cDNA clones (HGB.B1 and HGB.B2) encoding 94 amino acid-long isoforms were identified by screening a female harderian gland library with an HGB.B probe. The corresponding mature polypeptides are 78 amino acids long with 12 differences, but 3 putative N-glycosylation sites are maintained. The expression of HGB.B mRNAs is parallel to that of HGB and HGB.A, but no HGB.B2 mRNA was detected in submaxillary glands. Homology studies indicate that HGB.A and HGB.B1/HGB.B2 belong to different subfamilies of the secretoglobin-uteroglobin family and form heterodimers as previously described.
authors.affiliation: Departamento de Bioquimica y Biologia Molecular, Edificio Santiago Gascón, Universidad de Oviedo, 33006 Oviedo, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Alvarez
authors.forename: Jorge
authors.initials: J
authors.lastname: Viñas
authors.forename: José M Martín
authors.initials: JM
authors.lastname: Alonso
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Albar
authors.forename: Keith
authors.initials: K
authors.lastname: Ashman
authors.forename: Pedro
authors.initials: P
authors.lastname: Domínguez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification; D019281:Dimerization; D005260:Female; D006031:Glycosylation; D008297:Male; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009928:Organ Specificity; D020033:Protein Isoforms; D021122:Protein Subunits; D012333:RNA, Messenger / Q000032:analysis; D012727:Sex Characteristics; D014598:Uteroglobin / Q000032:analysis / Q000737:chemistry* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D020033:Protein Isoforms; D021122:Protein Subunits; D012333:RNA, Messenger; D014598:Uteroglobin