Comparison of a conventional immunoassay (ELISA) with a surface plasmon resonance-based biosensor for IGF-1 detection in cows' milk.
Recombinant bovine somatotropin (rBST) treatment is adopted in dairy cows to augment milk yield. Previous studies showed that insulin-like growth factor-1 (IGF-1) is present in milk from cows treated with rBST. Since IGF-1 is a suspected carcinogen, its presence in milk for human consumption is potentially a health hazard. Therefore rBST use, still authorized in the United States, has been revoked in Canada and is under evaluation in the EU. The rising attention on IGF-1 presence in alimentary milk focused the necessity to develop specific, sensitive and rapid IGF-1 detection systems. We have developed a solid phase enzyme-linked immunoassay (ELISA) and also an automated surface plasmon resonance-based biosensor system (BIA-technology) for evaluating IGF-1 in fresh cow's milk. Hyperimmune polyclonal anti-IGF-1 antibodies were characterized with respect to their specific binding capacity to IGF-1. The results obtained with these two methods have been compared. This study shows the potential usefulness of the biosensor technology for biomolecular interaction analysis. The features of this technology (fully automated, measures in real time, sharpened yes/no response) offer several advantages compared to ELISA in the detection of compounds in fresh cows' milk (MURST 40%; CNR P.F. MADESS 2).
Guidi
A
A
Department of Animal Pathology Prophylaxis and Food Hygiene, University of Pisa, Via delle Piaggie 2, 56100 Pisa, Italy. aguidi@vet.unipi.it
Laricchia-Robbio
L
L
Gianfaldoni
D
D
Revoltella
R
R
Del Bono
G
G
D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D013006:Growth Hormone / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000009:adverse effects / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D011994:Recombinant Proteins / Q000494:pharmacology; D020349:Surface Plasmon Resonance / Q000379:methods*
D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone