Immune phenotype and intracellular cytokine production of peripheral blood mononuclear cells from postmenopausal patients with osteoporotic fractures.
A number of factors with known effects on bone turnover are also immune regulatory factors. Disturbances of bone remodeling thus may be a consequence of altered local immune reactivity. We therefore determined surface markers and intracellular cytokine production of peripheral blood mononuclear cells by four-color flow cytometry in 19 postmenopausal patients with established osteoporosis and a control group of 11 postmenopausal women without fragility fractures. No significant differences in bone mineral density as assessed by dual energy X-ray absorptiometry were observed between the two groups. The following surface markers and cytokines were studied: CD3, CD4, CD8, CD16, CD19, CD29, CD45RA, CD56, CD57, HLA-DR, interleukin (IL)-1beta, IL-2, IL-4, IL-6, IL-13, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma and granulocyte macrophage colony stimulating factor. In the fracture patients, the percentage of CD8+ cells co-expressing CD57 was increased (14+/-2 vs. 8+/-1%; p=0.03). Moreover, the proportion of CD8+ cells co-expressing TNF-alpha (47+/-5 vs. 33+/-4; p=0.05) and both TNF-alpha and IFN-gamma was significantly higher in the patients than the controls (41+/-6 vs. 22+/-3%; p=0.04). IL-1beta expression tended to be increased in monocytes from patients with established osteoporosis. Distinct subsets of CD8+ cells thus appear to contribute to the development of osteoporotic fractures.
Pietschmann
P
P
Department of Pathophysiology, University of Vienna Medical School, Währinger Gürtel 18-20, A-1090 Vienna, Austria. peter.pietschmann@akh-wien.ac.at
Grisar
J
J
Thien
R
R
Willheim
M
M
Kerschan-Schindl
K
K
Preisinger
E
E
Peterlik
M
M
D000368:Aged; D018959:CD57 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D050723:Fractures, Bone / Q000209:etiology* / Q000503:physiopathology*; D006801:Humans; D007107:Immune System / Q000503:physiopathology*; D007375:Interleukin-1 / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D015663:Osteoporosis, Postmenopausal / Q000150:complications*; D010641:Phenotype; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D018959:CD57 Antigens; D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha