title: Site-based study on polymorphism of Plasmodium falciparum MSP-1 and MSP-2 genes in isolates from two villages in Central Africa.
abstract: We investigated Plasmodium falciparum genetic diversity in isolates collected from school-going residents aged from 5 to 15 years in the village of Pouma (Cameroon, Central Africa). Seventy-six children were grouped according to the clinical status. Asymptomatic status was defined as parasite carriage in the absence of any clinical symptom and malaria symptomatic status with patent parasitemia over 5000 parasites/microliter of blood and an axillary temperature > 37.5 degrees C. Parasite DNA was analysed prior to malaria treatment. Genotyping of the P. falciparum merozoite surface proteins (MSP) 1 and 2 was performed by polymerase chain reaction using allele-specific primers. K1, MAD20, Ro33 and 3D7/CAMP, FC27 allelic families were attributed to MSP-1 and MSP-2 genes, respectively. No association was found between P. falciparum MSP-1 and MSP-2 genotypes and the clinical status of children. Mixed P. falciparum infections were detected in 78% of overall samples and all isolates from symptomatic children contained more than 1 clone. The results obtained in the village of Pouma were compared to those of the village of Dienga in Gabon where a similar study, using the same genotyping methods, had been carried out in the same age group of schoolchildren. Data are interpreted in the context of malaria epidemiology in both settings.
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), BP 769, Franceville, Gabon. ffntoumi@hotmail.com
authors.forename: F
authors.initials: F
authors.lastname: Ntoumi
authors.forename: J
authors.initials: J
authors.lastname: Ngoundou-Landji
authors.forename: F
authors.initials: F
authors.lastname: Lekoulou
authors.forename: A
authors.initials: A
authors.lastname: Luty
authors.forename: P
authors.initials: P
authors.lastname: Deloron
authors.forename: P
authors.initials: P
authors.lastname: Ringwald
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002648:Child; D005681:Gabon; D017125:Genes, Protozoan / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D020066:Merozoite Surface Protein 1 / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium