Polymeric delivery of proteins and plasmid DNA for tissue engineering and gene therapy.
In vivo gene expression can be altered by locally delivered DNA and proteins. The ability to deliver bioactive macromolecules, such as proteins and plasmid DNA, over controllable time frames represents a challenging engineering problem. Considerable success has been achieved with polymeric delivery systems that provide the capability to change cell function either acutely or chronically. This review focuses on controlled delivery of proteins and plasmid DNA from polymers and on the effects of controlled delivery on gene expression, and introduces some cell biological and biochemical parameters to be considered when delivering macromolecules to change cell behavior.
Richardson
T P
TP
Department of Biomedical Engineering, University of Michigan, Ann Arbor 48109, USA.
Murphy
W L
WL
Mooney
D J
DJ
D000818:Animals; D001682:Biological Availability; D001704:Biopolymers / Q000008:administration & dosage*; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000008:administration & dosage; D004339:Drug Compounding; D016503:Drug Delivery Systems; D004355:Drug Stability; D005544:Forecasting; D005786:Gene Expression Regulation / Q000187:drug effects; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006016:Glycolates / Q000008:administration & dosage; D006133:Growth Substances / Q000008:administration & dosage / Q000627:therapeutic use; D006207:Half-Life; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage; D008863:Microspheres; D011091:Polyesters; D011100:Polyglycolic Acid / Q000008:administration & dosage; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage; D011506:Proteins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D023822:Tissue Engineering / Q000379:methods*
D001704:Biopolymers; D004274:DNA, Recombinant; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006016:Glycolates; D006133:Growth Substances; D011091:Polyesters; D011108:Polymers; D011506:Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)