title: Gi- and Gs-coupled receptors up-regulate the cAMP cascade to modulate HCN2, but not HCN1 pacemaker channels.
abstract: A hallmark of native pacemaker channels is their regulation by neurotransmitters and hormones acting through the second messenger cAMP. In this study, we investigated the modulation of two cloned pacemaker channels, HCN1 and HCN2, by activation of coexpressed inhibitory G protein (Gi)-coupled (p-opioid) or stimulatory G protein (Gs)-coupled [serotonin 5-HT4(a)] receptors in Xenopus oocytes. Both receptors enhanced HCN2, but not HCN1 currents. Receptor activation increased HCN2 current amplitude, increased the activation rate sixfold and decreased the deactivation rate two-fold. In addition, the fully-activated current for HCN2 increased due to a receptor-induced increase of the maximal conductance. These effects were inhibited by 9-(tetrahydro-2'-furyl)adenine (SQ22536), were independent of protein kinases A and C and could be explained by a cAMP-induced shift of the voltage dependence of activation by 15 mV to more positive potentials. The pathway through which these effects occurred involved Gbetagamma-activation of adenylyl cyclase and, in the case of the p-opioid receptor, required co-expression of Galphas. The effect of the 5-HT4(a)-receptor, in part caused by its constitutive activity, occurred directly through Galphas-activation. This suggests that 5-HT4(a) receptors may contribute to functional heterogeneity of pacemaker currents (Ih) in those neurons in which 5-HT4(a)R and HCN2 coexist.
authors.affiliation: Laboratory of Toxicology, University of Leuven, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Ulens
authors.forename: J
authors.initials: J
authors.lastname: Tytgat
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D054815:Cyclic Nucleotide-Gated Cation Channels; D004553:Electric Conductivity; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000494:pharmacology; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology*; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels / Q000235:genetics / Q000502:physiology*; D008564:Membrane Potentials; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D009865:Oocytes / Q000378:metabolism; D015221:Potassium Channels; D011493:Protein Kinase C / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000502:physiology*; D011985:Receptors, Serotonin / Q000502:physiology*; D014162:Transfection; D014981:Xenopus
chemical_list: D054815:Cyclic Nucleotide-Gated Cation Channels; C578665:HCN1 protein, human; C578710:HCN2 protein, human; D064428:Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; D007473:Ion Channels; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D017450:Receptors, Opioid, mu; D011985:Receptors, Serotonin; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; C017759:9-(tetrahydro-2-furyl)-adenine; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019205:GTP-Binding Protein alpha Subunits, Gs; D000225:Adenine