Use of a thin film biosensor for rapid visual detection of PCR products in a multiplex format.
Rapid, sensitive assays for nucleic acid amplification products have utility for the identification of bacterial or viral infections. We have developed a nucleic acid hybridization assay utilizing thin film technology that permits visual detection of hybrids. The silicon-based biosensor detects the presence of target sequences by enzymatically transducing the formation of nucleic acid hybrids into molecular thin films. These films alter the interference pattern of light on the biosensor surface, producing a perceived color change. We have applied this technology to the development of a chip containing capture probes specific for human respiratory virus sequences including respiratory syncytial virus, influenza virus A and B, parainfluenza virus types 1 and 3, and rhinovirus. In a ten-minute assay, the biosensor permits unambiguous identification of viral-specific RT/PCR products from infected cell lysates.
Jenison
R
R
ThermoBioStar, Inc., 6655 Lookout Road, Boulder, CO 80301, USA.
Rihanek
M
M
Polisky
B
B
D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D004867:Equipment Design; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics; D012141:Respiratory Tract Infections / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012825:Silicon; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification
D015345:Oligonucleotide Probes; D012825:Silicon