A new panoptic stain for developmental biology--the mouse placenta paradigm.
A panoptic histological stain, PHTA, is introduced for routine use in developmental biology. The protocol is based on three dyes, hematoxylin and, after tannic acid treatment, phloxine B and azure B. It was optimized for differential staining of extracellular matrix, cytoplasm and chromatin. The method is quick, inexpensive and well-reproducible. The mouse placenta is used here to demonstrate the excellent suitability of PHTA for routine morphology.
Kurz
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Institute of Anatomy II, University of Freiburg, Albertstrasse 17, D-79104 Freiburg, Germany. kurzhaym@uni-freiburg.de
Wittekind
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D000818:Animals; D004396:Coloring Agents; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010920:Placenta / Q000166:cytology*
D004396:Coloring Agents; D005456:Fluorescent Dyes