Rapid quantitative determination and assessment of insulin in oil formulation by micellar electrokinetic capillary chromatography.
A rapid, simple and precise micellar electrokinetic capillary chromatrography (MEKC) with diode array detector had been developed to the determination of insulin in oil formulation. As the micelle-forming ampholytic surfactant, Labrasol was chosen for the separation and analysis of the hydrophobic insulin in oil formulation in this experiment. A buffer containing 10 mmol/ml Tris-HCl plus 10% acetonitrile (ACN) at pH 8.2 was used for running buffer. Samples and tested samples were successfully separated during 8 min with a good linear range(r>0.99) and a concentration detection limit of 0.01 mg/ml. Furthermore, quantitative determinations of insulin in oil formulation had been described and assessed after handled with different pre-treatment conditions to reveal the effect of pH, temperature and tryptic digestion.
Deng
Bin
B
Analytical Center, School of Life Science and Engineering, Tsinghua University, 100084, Beijing, People's Republic of China.
Liu
Zheng
Z
Luo
Guoan
G
Ma
Hong
H
Duan
Mingxing
M
D002626:Chemistry, Pharmaceutical; D002845:Chromatography / Q000379:methods; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis*; D008823:Micelles; D009821:Oils / Q000032:analysis; D015203:Reproducibility of Results; D013696:Temperature; D014357:Trypsin
D004304:Dosage Forms; D007328:Insulin; D008823:Micelles; D009821:Oils; D014357:Trypsin