Developmental regulation of DNA replication timing at the human beta globin locus.
The human beta globin locus replicates late in most cell types, but becomes early replicating in erythroid cells. Using FISH to map DNA replication timing around the endogenous beta globin locus and by applying a genetic approach in transgenic mice, we have demonstrated that both the late and early replication states are controlled by regulatory elements within the locus control region. These results also show that the pattern of replication timing is set up by mechanisms that work independently of gene transcription.
Simon
I
I
Department of Cellular Biochemistry, Hebrew University Medical School, Jerusalem, Israel.
Tenzen
T
T
Mostoslavsky
R
R
Fibach
E
E
Lande
L
L
Milot
E
E
Gribnau
J
J
Grosveld
F
F
Fraser
P
P
Cedar
H
H
D000818:Animals; D002478:Cells, Cultured; D002880:Chromosomes, Human, Pair 11; D002999:Clone Cells; D004261:DNA Replication / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019901:Locus Control Region / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D013997:Time Factors; D019076:Transgenes
D005914:Globins