Analysis of a 1-Mb BAC contig overlapping the mouse Nkrp1 cluster of genes: cloning of three new Nkrp1 members, Nkrp1d, Nkrp1e, and Nkrp1f.
The murine Nkrp1 gene family encodes three previously identified activation and inhibitory receptors expressed on natural killer (NK) cells. This family includes the gene for NKR-P1C (NK1.1), the most specific serologic marker on C57BL/6-derived NK cells and is localized in a gene cluster in the NK gene complex (NKC). To further analyze the Nkrp1 family, we constructed and analyzed a bacterial artificial chromosome contig. A genomic organization of the Nkrp1 family was obtained and three new Nkrp1 genes were isolated from interleukin-2-activated NK cells. Thus, the Nkrp1 family adds to the repertoire of receptors expressed by NK cells.
Plougastel
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B
Howard Hughes Medical Institute, Division of Rheumatology, Washington University, Box 8045, 660 S. Euclid Avenue, Saint Louis, MO 63110, USA. bplougas@im.wustl.edu
Matsumoto
K
K
Dubbelde
C
C
Yokoyama
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WM
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping; D016678:Genome; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011544:Pseudogenes; D011971:Receptors, Immunologic / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011971:Receptors, Immunologic; D000099192:CD69 Antigens; C527212:Klrb1b protein, mouse; C527211:Klrb1c protein, mouse; C442138:Nkrp1f protein, mouse