The influence of medium conductivity on electropermeabilization and survival of cells in vitro.
Electropermeabilization and cell death caused by the exposure to high voltage electric pulses depends on the parameters of pulses, as well as the composition of the extracellular medium. We studied the influence of extracellular conductivity on electropermeabilization and survival of cells in vitro. For this purpose, we used a physiological medium with a conductivity of 1.6 S/m and three artificial media with conductivities of 0.14, 0.005, and 0.001 S/m. Measurements of pH, osmolarity, and cell diameter were made to estimate possible side effects of the media on the cells. Our study shows that the percentage of surviving cells increases with the decreasing medium conductivity, while the percentage of electropermeabilized cells remains unaffected. Our results show that cell survival in experiments involving electropermeabilization can be improved by decreasing the medium conductivity. To provide an interpretation of experimental results, we have theoretically estimated the resting transmembrane voltage, the induced transmembrane voltage, the time constant of the voltage inducement, and heating of the cell suspension for each of the media used. These calculations imply that for accurate interpretation of experimental results, both the induced and the resting transmembrane voltage must be considered, taking into account the conductivity and the ionic composition of the extracellular medium.
Pucihar
G
G
Laboratory of Biocybernetics, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI-1000, Ljubljana, Slovenia.
Kotnik
T
T
Kanduser
M
M
Miklavcic
D
D
D000818:Animals; D002461:Cell Line, Transformed / Q000166:cytology*; D048429:Cell Size; D002470:Cell Survival; D006224:Cricetinae; D003470:Culture Media / Q000494:pharmacology; D004553:Electric Conductivity; D018274:Electroporation / Q000379:methods / Q000592:standards*; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D008954:Models, Biological; D009994:Osmolar Concentration
D003470:Culture Media