Cisplatin ototoxicity in the Sprague Dawley rat evaluated by distortion product otoacoustic emissions.
The present study has evaluated the use of distortion product otoacoustic emission (DPOAE) responses in the detection of cisplatin-induced ototoxicity in a Sprague Dawley rat animal model. The cisplatin was administered as a 16 mg/kg, dose introduced by a slow 30-min intraperitoneal infusion. Data from three DP-gram protocols, DPOAE input-output responses at 8 kHz, and auditory brainstem responses (ABRs) at 8, 12 and 16 kHz were collected before and 72 h after treatment. The post-treatment ABRs at 16 kHz showed the greatest mean threshold shift of 33.6 dB. The post-treatment DP-gram data showed significant reduction of the signal to noise ratios in the majority of the frequencies tested, across all tested protocols. The data suggest that the most sensitive DPOAE procedure for the early detection of the cisplatin-induced ototoxic damage is the DPOAE I/O protocol. Morphological analyses indicated that the inner hair cells remained intact, while several types of alterations were observed in the arrangement of the stereocilia in the outer hair cells.
Hatzopoulos
S
S
Department of Audiology and Centre of Bioacoustics, University of Ferrara, Italy.
Di Stefano
M
M
Campbell
K C
KC
Falgione
D
D
Ricci
D
D
Rosignoli
M
M
Finesso
M
M
Albertin
A
A
Previati
M
M
Capitani
S
S
Martini
A
A
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects*; D001307:Auditory Perception / Q000187:drug effects; D001309:Auditory Threshold / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage / Q000009:adverse effects*; D003051:Cochlea / Q000187:drug effects* / Q000648:ultrastructure; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000648:ultrastructure; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000648:ultrastructure; D007274:Injections, Intraperitoneal; D008297:Male; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000970:Antineoplastic Agents; D002945:Cisplatin