Dynamic flexibility in the Escherichia coli genome.
Empirical rules based on tetranucleotide parameters were presented to predict the structural parameters twist (Omega), roll (rho), tilt (tau) and slide (D(y)). A statistical mechanical model was used to analyze the flexibility of the Escherichia coli genome. The replication terminus region displayed a low level of flexibility. A strong correlation can be seen between G+C content and flexibility. Average flexibilities in the coding regions were found to be significantly larger than those in non-coding regions. The flexible characteristics in the 5'-neighborhood of the coding regions and in three class sigma promoter sequences in the E. coli genome were also analyzed.
Tsai
L
L
Department of Biological Science and Technology, Tsinghua University, Beijing, PR China.
Sun
Z
Z
D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D018583:Pliability