Detection and quantification of snow algae with an airborne imaging spectrometer.
We describe spectral reflectance measurements of snow containing the snow alga Chlamydomonas nivalis and a model to retrieve snow algal concentrations from airborne imaging spectrometer data. Because cells of C. nivalis absorb at specific wavelengths in regions indicative of carotenoids (astaxanthin esters, lutein, beta-carotene) and chlorophylls a and b, the spectral signature of snow containing C. nivalis is distinct from that of snow without algae. The spectral reflectance of snow containing C. nivalis is separable from that of snow without algae due to carotenoid absorption in the wavelength range from 0.4 to 0.58 microm and chlorophyll a and b absorption in the wavelength range from 0.6 to 0.7 microm. The integral of the scaled chlorophyll a and b absorption feature (I(0.68)) varies with algal concentration (C(a)). Using the relationship C(a) = 81019.2 I(0.68) + 845.2, we inverted Airborne Visible Infrared Imaging Spectrometer reflectance data collected in the Tioga Pass region of the Sierra Nevada in California to determine algal concentration. For the 5.5-km(2) region imaged, the mean algal concentration was 1,306 cells ml(-1), the standard deviation was 1,740 cells ml(-1), and the coefficient of variation was 1.33. The retrieved spatial distribution was consistent with observations made in the field. From the spatial estimates of algal concentration, we calculated a total imaged algal biomass of 16.55 kg for the 0.495-km(2) snow-covered area, which gave an areal biomass concentration of 0.033 g/m(2).
Painter
T H
TH
Institute for Computational Earth System Science, University of California, Santa Barbara, California 93106, USA. painter@icess.ucsb.edu
Duval
B
B
Thomas
W H
WH
Mendez
M
M
Heintzelman
S
S
Dozier
J
J
D000401:Aircraft / Q000295:instrumentation*; D000531:Altitude; D000818:Animals; D018533:Biomass; D002696:Chlamydomonas / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D007091:Image Processing, Computer-Assisted; D012914:Snow; D013055:Spectrophotometry, Infrared / Q000379:methods; D014481:United States; D018557:United States National Aeronautics and Space Administration