Anticlastogenic effect of Vitamin C on cisplatin induced chromosome aberrations in human lymphocyte cultures.
Vitamin C (ascorbic acid) is an antioxidant that can scavenge free radicals and protect cellular macromolecules, including DNA, from oxidative damage induced by different agents. The protective effect of Vitamin C on cisplatin induced chromosome aberrations has been determined in the human peripheral lymphocyte chromosome aberration test in vitro. The results of treatments with Vitamin C indicated that it statistically significantly decreases the number of chromosome aberrations and number of metaphases with aberrations induced with cisplatin, but it can not completely protect cells from damage. The test concentrations of Vitamin C (10 and 100 microg/ml) had a limited antimutagen effect on cisplatin (0.5 microg/ml), which can cause genetic damage through free radical mechanisms. The antimutagen effect included the anticlastogenic effect of Vitamin C and its ability to decrease the number of aneuploid mitoses. Vitamin C showed the most efficient anticlastogenic effect during simultaneous treatment with cisplatin. Also, Vitamin C reduced cell toxicity of cisplatin during simultaneous treatment.
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Department of Genetics, Faculty of Science Sarajevo, Zmaja od Bosne 33-35, Sarajevo 71000, Bosnia and Herzegovina. hilada@bih.net.ba
D000782:Aneuploidy; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002945:Cisplatin / Q000037:antagonists & inhibitors* / Q000633:toxicity; D020732:Cytogenetic Analysis; D019610:Cytoprotection / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*
D000975:Antioxidants; D009153:Mutagens; D001205:Ascorbic Acid; D002945:Cisplatin