title: Sequence of the pig major histocompatibility region containing the classical class I genes.
abstract: A segment comprising 307,078 nucleotides of the pig major histocompatibility complex (SLA) was completely sequenced. The segment corresponded to the entire SLA classical class I-containing region of the serologically defined SLA H01 haplotype. In all, 11 genes were characterized, comprising 7 class I genes located on the centromeric part of the sequence (SLA-1, 2, 3, 4, 5, 9, and 11) and 4 ring finger-related family genes located on its telomeric part. No member of one family was intermingled with a member of the other or with any third-party gene. All class I genes except SLA-11 were similarly orientated. The SLA-1, 2, and 3 genes displayed both promoter and overall coding regions compatible with normal functions. The SLA-4, 11, and 9 genes were considered pseudogenes because they exhibited marked anomalies. Although the SLA-5 gene had a complete coding region, it displayed mutations in promoter elements which could modify its expression. The great molecular similarity observed among the class I genes extended far outside them, and resulted from segmental duplications. The ring finger genes exhibited great homology with their human counterparts. In pig, one of these genes appeared to correspond to a complete gene which in humans is probably a pseudogene. In all, the 11 genes characterized span about 20% of the total sequence. The remaining 80% consists of interspersed repeat elements. The present results, together with the sequence previously reported involving the SLA class I-related genes, open the way for a better understanding of pig MHC organization.
authors.affiliation: Laboratoire de Radiobiologie et d'Etude du Génome, INRA-CEA, Domaine de Vilvert, 78352 Jouy-en-Josas cedex, France. crenard@biotec.jouy
authors.forename: C
authors.initials: C
authors.lastname: Renard
authors.forename: M
authors.initials: M
authors.lastname: Vaiman
authors.forename: N
authors.initials: N
authors.lastname: Chiannilkulchai
authors.forename: L
authors.initials: L
authors.lastname: Cattolico
authors.forename: C
authors.initials: C
authors.lastname: Robert
authors.forename: P
authors.initials: P
authors.lastname: Chardon
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005805:Genes, MHC Class I; D006239:Haplotypes; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*; D016615:Telomere / Q000235:genetics; D020506:Untranslated Regions; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D015395:Histocompatibility Antigens Class I; D020506:Untranslated Regions