Interaction of zyxin, a focal adhesion protein, with the e6 protein from human papillomavirus type 6 results in its nuclear translocation.
Zyxin, a focal adhesion molecule, interacts specifically with the E6 protein from human papillomavirus (HPV) type 6 in a yeast two-hybrid screen of a cDNA library prepared from human keratinocytes. Zyxin does not interact significantly with E6 proteins from HPV types 11, 16, or 18. The interaction was confirmed by in vitro and in vivo analyses and it requires the LIM domains (Lin-11, Isl-1, and Mec-3 [G. Freyd, S. K. Kim, and H. R. Horvitz, Nature 344:876-879, 1990]) found at the carboxyl terminus of zyxin. Cotransfection of E6 from HPV ((6)E6) and zyxin results in the accumulation of zyxin in the nucleus where it can function as a transcriptional activator. (6)E6 can also mobilize endogenous zyxin to the nucleus.
Degenhardt
Y Y
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Department of Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Silverstein
S
S
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D027383:Papillomaviridae / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D060589:Zyxin
D003598:Cytoskeletal Proteins; D017931:DNA Primers; C118860:E6 protein, Human papillomavirus type 6; D006023:Glycoproteins; D008667:Metalloproteins; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C092842:ZYX protein, human; D060589:Zyxin