title: Japanese family with glucocorticoid-remediable aldosteronism diagnosed by long-polymerase chain reaction.
abstract: We report a Japanese family with glucocorticoid-remediable aldosteronism (GRA) in whom gene abnormality was identified by the long-polymerase chain reaction (PCR) method. The proband was a 21-year-old female incidentally found to have high blood pressure (173/107 mmHg). Laboratory tests showed hypokalemia (3.7 mmol/l), and high plasma aldosterone concentration (PAC, 234 pg/ml) with suppressed plasma renin activity (PRA, <0.1 ng/ml/h). The circadian rhythm pattern and the results of a rapid adrenocorticotrophic hormone (ACTH) test indicated ACTH-dependent changes in PAC. Imaging studies showed no adrenal mass on either side. A dexamethasone (Dexa) suppression test (1.0 mg/day orally for 7 days) showed a marked decrease of PAC 2 days after administration, and this decreased level was maintained throughout Dexa administration. High blood pressure and hypokalemia also improved during Dexa treatment. The proband's younger sister was 19 years old and had hypertension, PAC of 231 pg/ml, and PRA <0.1 ng/ml/h. The mother was 53 years old and had hypertension, PAC of 98.5 pg/ml, and PRA <0.1 ng/ml/h. The proband's elder sister was a 22-year-old normotensive with PAC of 110 pg/ml and PRA of 0.1 ng/ml. Long-PCR was performed for detection of the chimeric gene associated with GRA, using DNA samples from all four cases and two normal control subjects. Although the aldosterone synthase gene was expressed among all DNA samples, the chimeric gene was detected only in the proband, her younger sister and her mother. Our clinical data and genetic investigation confirmed the presence of GRA in this Japanese family.
authors.affiliation: Department of Medicine III, Okayama University Medical School, Japan. endocrin@cc.okayama-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Yokota
authors.forename: T
authors.initials: T
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Kishida
authors.forename: J
authors.initials: J
authors.lastname: Suzuki
authors.forename: F
authors.initials: F
authors.lastname: Otsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Mimura
authors.forename: T
authors.initials: T
authors.lastname: Oishi
authors.forename: M
authors.initials: M
authors.lastname: Hirata
authors.forename: K
authors.initials: K
authors.lastname: Tobe
authors.forename: H
authors.initials: H
authors.lastname: Makino
mesh_terms: D000328:Adult; D002678:Chimera; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics; D003907:Dexamethasone; D005192:Family Health; D005260:Female; D005938:Glucocorticoids; D006801:Humans; D006929:Hyperaldosteronism / Q000175:diagnosis* / Q000235:genetics*; D006973:Hypertension / Q000235:genetics; D007564:Japan; D008875:Middle Aged; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods*; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase