title: Biflavonoids inhibit the production of aflatoxin by Aspergillus flavus.
abstract: The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 microg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 microg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production.
authors.affiliation: Laboratório de Produtos Naturais, Centro de Sanidade Animal, Instituto Biológico, Av. Cons. Rodrigues Alves, 1252, 04014-002 São Paulo, SP, Brazil. goncalez@biologico.br
authors.forename: E
authors.initials: E
authors.lastname: Gonçalez
authors.forename: J D
authors.initials: JD
authors.lastname: Felicio
authors.forename: M M
authors.initials: MM
authors.lastname: Pinto
mesh_terms: D016604:Aflatoxin B1 / Q000096:biosynthesis*; D000348:Aflatoxins / Q000096:biosynthesis*; D000704:Analysis of Variance; D001231:Aspergillus flavus / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000348:Aflatoxins; D005419:Flavonoids; D010936:Plant Extracts; C029753:aflatoxin B2; D016604:Aflatoxin B1