Towards the reconstruction of central nervous system white matter using neural precursor cells.
Epidermal growth factor-responsive neural precursor cells were used as donor cells for transplantation into wild-type and myelin-deficient shiverer (shi) mice. The cells engrafted robustly within the CNS following intracerebroventricular and cisternal transplantation in neonatal mice. The cells adopted glial phenotypes, and some functioned as oligodendrocytes, producing myelin basic protein and morphologically normal internodal myelin sheaths. When individual shi mice received two transplants (on post-natal days 1 and 3), donor-derived cells disseminated widely and expressed myelin basic protein in central white matter tracts throughout the brain.
Mitome
M
M
Department of Neurology, University of Massachusetts Medical School, Worcester 01655, USA.
Low
H P
HP
van den Pol
A
A
Nunnari
J J
JJ
Wolf
M K
MK
Billings-Gagliardi
S
S
Schwartz
W J
WJ
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000637:transplantation* / Q000648:ultrastructure; D001369:Axons / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology* / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D003342:Corpus Striatum / Q000187:drug effects / Q000637:transplantation; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D007276:Injections, Intraventricular; D020547:Lateral Ventricles / Q000187:drug effects / Q000637:transplantation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D004676:Myelin Basic Protein / Q000096:biosynthesis; D009186:Myelin Sheath / Q000502:physiology; D009836:Oligodendroglia / Q000187:drug effects / Q000378:metabolism / Q000637:transplantation* / Q000648:ultrastructure; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000187:drug effects / Q000648:ultrastructure
D004676:Myelin Basic Protein; D004815:Epidermal Growth Factor