title: Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.
abstract: The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.
authors.affiliation: U.S. Department of Agriculture-Agricultural Research Service, Beltsville, Maryland 20705, USA. jhiggins@anri.barc.usda.gov
authors.forename: J A
authors.initials: JA
authors.lastname: Higgins
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
authors.forename: D R
authors.initials: DR
authors.lastname: Shelton
authors.forename: R
authors.initials: R
authors.lastname: Fayer
authors.forename: J S
authors.initials: JS
authors.lastname: Karns
mesh_terms: D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000254:growth & development; D004269:DNA, Bacterial / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000254:growth & development; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan