title: Deficit in oxygen causes G(2) budding and unbudded G(2) arrest in Cryptococcus neoformans.
abstract: Cryptococcus neoformans exhibited diphasic growth when grown under limited aeration. First, it grew exponentially, but at OD 1, the concentration of dissolved oxygen in culture decreased to 1 mg l(-1) and a second phase of slow growth was started. This phase was characterized by a shift of budding from S to G(2), a sharp decrease in budding index and a sharp increase in the proportion of unbudded G(2) cells to 80%. Thus, a deficit in oxygen was demonstrated to delay the timing of budding, prolong the G(2) phase and cause accumulation of cells after DNA synthesis, but before commitment to budding.
authors.affiliation: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohkusu
authors.forename: V
authors.initials: V
authors.lastname: Raclavsky
authors.forename: K
authors.initials: K
authors.lastname: Takeo
mesh_terms: D002453:Cell Cycle; D003455:Cryptococcus neoformans / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D003470:Culture Media; D004271:DNA, Fungal / Q000032:analysis; D016195:G2 Phase / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D005947:Glucose; D010100:Oxygen