title: Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.
abstract: DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
authors.affiliation: The National Diagnostics Centre, BioResearch Ireland, National University of Ireland. Evelyn.Collins@nuigalway.ie
authors.forename: E
authors.initials: E
authors.lastname: Collins
authors.forename: M
authors.initials: M
authors.lastname: Glennon
authors.forename: S
authors.initials: S
authors.lastname: Hanley
authors.forename: A M
authors.initials: AM
authors.lastname: Murray
authors.forename: M
authors.initials: M
authors.lastname: Cormican
authors.forename: T
authors.initials: T
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Maher
mesh_terms: D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology*; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003124:Colorimetry; D015342:DNA Probes / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008567:Membranes, Artificial; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D008567:Membranes, Artificial; D011933:Reagent Kits, Diagnostic