Oral administration of IL-12 suppresses anaphylactic reactions in a murine model of peanut hypersensitivity.
There is no satisfactory therapeutic intervention for peanut allergy, which accounts for most life-threatening food allergic reactions. Since IL-12 has been found to inhibit allergic airway responses in a mouse model of asthma and to cure Th2 cytokine-mediated murine schistosomiasis, we hypothesized that IL-12 treatment might also inhibit peanut allergic reactions. Consequently, we investigated the effects of oral IL-12 treatment in a murine model of peanut allergy and found that oral administration of liposome encapsulated rIL-12 could both prevent and reverse peanut hypersensitivity and could reduce histamine release, peanut-specific serum IgE and IgG1, and fecal IgA levels. Oral IL-12 treatment also increased IFN-gamma but did not decrease IL-4 or IL-5 levels. We conclude that oral rIL-12 treatment has therapeutic as well as preventive effects on peanut allergy, which are associated with increased IFN-gamma production.
Lee
S Y
SY
Department of Pediatrics, Mount Sinai School of Medicine, New York, NY 10029, USA.
Huang
C K
CK
Zhang
T F
TF
Schofield
B H
BH
Burks
A W
AW
Bannon
G A
GA
Sampson
H A
HA
Li
X M
XM
D000284:Administration, Oral; D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D010367:Arachis / Q000276:immunology; D005260:Female; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000145:classification; D007371:Interferon-gamma / Q000032:analysis; D018664:Interleukin-12 / Q000008:administration & dosage*; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D021183:Peanut Hypersensitivity / Q000188:drug therapy*
D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D015848:Interleukin-5; D018664:Interleukin-12; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma