Rapid and accurate determination of genotypic clarithromycin resistance in cultured Helicobacter pylori by fluorescent in situ hybridization.
Phenotypic susceptibility testing for clarithromycin by E-test and disk diffusion of 109 cultured Helicobacter pylori isolates was compared with the genotypic susceptibility determination by fluorescent in situ hybridization (FISH). No discrepancies were found between these three methods. However, FISH has the advantage of providing results after 3 h.
Rüssmann
H
H
Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians-Universität München, 80336 Munich, Germany. ruessmann@m3401.mpk.med.uni-muenchen.de
Adler
K
K
Haas
R
R
Gebert
B
B
Koletzko
S
S
Heesemann
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017291:Clarithromycin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008826:Microbial Sensitivity Tests / Q000379:methods; D013997:Time Factors
D000900:Anti-Bacterial Agents; D017291:Clarithromycin