Single molecule detector for mass spectrometry with mass independent detection efficiency.
Experimental results from equimolar PEG and protein standards samples are presented from a MALDI-TOF mass spectrometer equipped with both ionizing detectors and the novel single molecule sensitive cryodetectors. The data are consistent with a model hypothesis suggesting that the observed decrease in signal strength in conventional ionizing detector MALDI-TOF mass spectrometers can be explained by the exponentially decreasing quantum efficiency of ionizing detectors. Cryodetectors, in contrast, have a mass independent detection efficiency of 100% on impact and provide additional information on the molecule state owing to the calorimetric nature of the detection mechanism.
Twerenbold
D
D
Institut de Physique, Université Neuchâtel, Switzerland. twerenbold@genspec.ch
Gerber
D
D
Gritti
D
D
Gonin
Y
Y
Netuschill
A
A
Rossel
F
F
Schenker
D
D
Vuilleumier
J L
JL
D000535:Aluminum; D002151:Calorimetry; D011092:Polyethylene Glycols / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*
D020543:Proteome; D011092:Polyethylene Glycols; D000535:Aluminum