title: Local expression and distribution of a storage protein in the ovary of Hyphantria cunea.
abstract: Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study, the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and immunocytochemical methods. HcSP-1 was detected in the ovaries in 6-day-old pupae and accumulated toward the end of pupal life, when HcSP-1 transcripts were detectable by Northern blot analysis and RT-PCR. In situ hybridization showed that the HcSP-1 mRNA was located in the nurse cells and follicular epithelial cells, but not in the oocyte. Though most of the HcSP-1 that is incorporated in the yolk bodies of the oocyte is probably sequestered from the surrounding hemolymph, HcSP-1 is an important yolk protein contributing to early yolk body formation before the development of patency by the follicular epithelium.
authors.affiliation: Division of Life Science, College of Natural Sciences, Gyeongsang National University, Chinju, Korea.
authors.forename: H M
authors.initials: HM
authors.lastname: Cheon
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: D H
authors.initials: DH
authors.lastname: Chung
authors.forename: M O
authors.initials: MO
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: C Y
authors.initials: CY
authors.lastname: Yun
authors.forename: S J
authors.initials: SJ
authors.lastname: Seo
mesh_terms: D000818:Animals; D005216:Fat Body / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D009036:Moths / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; C041137:storage proteins, Insecta