title: New perspectives in the Escherichia coli proteome investigation.
abstract: Escherichia coli is a model organism for biochemical and biological studies as it is one of the best characterised prokaryote. Two-dimensional polyacrylamide gel electrophoresis, computer image analysis and different protein identification techniques gave rise, in 1995, to the Escherichia coli SWISS-2D PAGE database (http://www.expasy.ch/ch2d/). In the E. coli 3.5-10 SWISS-2D PAGE map, 40% of the E. coli proteome was displayed. The present study demonstrated that the use of narrow range pH gradients is able to potentially display up to a few copies of protein per E. coli cell. Moreover, the six new E. coli SWISS-2D PAGE maps (pH 4-5, 4.5-5.5, 5-6, 5.5-6.7, 6-9 and 6-11) presented here displayed altogether more than 70% of the entire E. coli proteome.
authors.affiliation: Central Clinical Chemistry Laboratory, Geneva University Hospital, 24 Rue Michell-du-Crest, 1211 Geneva 14, Switzerland. juisa.tonella@dim.hcuge.ch
authors.forename: L
authors.initials: L
authors.lastname: Tonella
authors.forename: C
authors.initials: C
authors.lastname: Hoogland
authors.forename: P A
authors.initials: PA
authors.lastname: Binz
authors.forename: R D
authors.initials: RD
authors.lastname: Appel
authors.forename: D F
authors.initials: DF
authors.lastname: Hochstrasser
authors.forename: J C
authors.initials: JC
authors.lastname: Sanchez
mesh_terms: D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000302:isolation & purification*; D010449:Peptide Mapping; D020543:Proteome / Q000302:isolation & purification*
chemical_list: D029968:Escherichia coli Proteins; D020543:Proteome