Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8.
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.
Pettinari
M J
MJ
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Vázquez
G J
GJ
Silberschmidt
D
D
Rehm
B
B
Steinbüchel
A
A
Méndez
B S
BS
D000102:Acetyl-CoA C-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D001395:Azotobacter / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003001:Cloning, Molecular; D017353:Gene Deletion; D005798:Genes, Bacterial; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D011091:Polyesters / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D000094122:Polyhydroxybutyrates
D000102:Acetyl-CoA C-Acyltransferase; D000217:Acyltransferases; D000429:Alcohol Oxidoreductases; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023744:acetoacetyl-CoA reductase; C060970:poly-beta-hydroxybutyrate polymerase