Immunocytochemistry in effusion cytology: a contemporary review.
BACKGROUND
Cytology plays a pivotal role in the diagnosis of pleural effusions. In many cases, immunocytochemistry (ICC) is required to elucidate the etiology of the atypical cells. Effusions are samples that present unique problems for ICC. To date there is no standardization of ICC methods for effusions and cytology in general.


METHODS
The authors review the most commonly used cytologic preparations, fixatives, and antibodies used in effusion ICC.


RESULTS
Through the utilization of cell block preparations and a panel of antibodies appropriate for the differential diagnosis in question, ICC conditions utilized in surgical pathology can be most closely replicated.


CONCLUSIONS
ICC may provide reliable insights into various diagnostic dilemmas in effusion cytology, provided that laboratory standardization practices are followed.
Fetsch
P A
PA
Cytopathology Section, National Institutes of Health/National Cancer Institute, Bethesda, Maryland 20892-1500, USA.
Abati
A
A
D000230:Adenocarcinoma / Q000473:pathology; D000912:Antibodies, Neoplasm; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry / Q000592:standards*; D008654:Mesothelioma / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000166:cytology*
D000912:Antibodies, Neoplasm