8-Prenylnaringenin, the phytoestrogen in hops and beer, upregulates the function of the E-cadherin/catenin complex in human mammary carcinoma cells.
The E-cadherin/catenin complex is a powerful invasion suppressor in epithelial cells. It is expressed in the human MCF-7 breast cancer cell line family, but functionally defective in the invasive MCF-7/6 variant. Previous experiments have shown that IGF-I, tamoxifen, retinoic acid and tangeretin are able to upregulate the function of this complex in MCF-7/6 cells. We investigated the effect of 8-prenylnaringenin (8-PN), the phytoestrogen present in hops and beer, on aggregation, growth and invasion in MCF-7/6 cells. 8-PN was found to stimulate E-cadherin-dependent aggregation and growth of MCF-7/6 cells in suspension. These effects could be inhibited by the pure anti-estrogen ICI 182,780. 8-PN did not affect invasion of MCF-7/6 cells in the chick heart assay in vitro. In all these aspects 8-PN mimics the effects of 17beta-estradiol on MCF-7/6 cells.
Rong
H
H
Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, University Gent, Belgium.
Boterberg
T
T
Maubach
J
J
Stove
C
C
Depypere
H
H
Van Slambrouck
S
S
Serreyn
R
R
De Keukeleire
D
D
Mareel
M
M
Bracke
M
M
D001515:Beer; D001943:Breast Neoplasms; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects*; D002449:Cell Aggregation / Q000187:drug effects*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D027582:Humulus / Q000737:chemistry*; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D015854:Up-Regulation
C119737:8-prenylnaringenin; D015820:Cadherins; D004968:Estrogens, Non-Steroidal; D044950:Flavanones; D005419:Flavonoids; D007529:Isoflavones; D048789:Phytoestrogens; D028321:Plant Preparations