[Identification of Amomum villosum, Amomum villosum var. xanthioides and Amomum longiligulare on ITS-1 sequence].
OBJECTIVE
To identify Amomum villosum Lour. and some their adulterants on molecular biology.


METHODS
The DNA of Amomum villosum Lour. and some their adulterants were extracted, and amplified using ITS-1 primer. The amplificed DNA were purified and then sequenced by direct PCR sequencing method.


RESULTS
The ITS-sequence of all of the samples are 248 bp in size. But there are 7 bases in Amomum villosum Lour var. xanthioides (Wall.ex Bak) T.L. Wu et Senjen and 12 hases in Amomum longiligulare T.L. Wu. differing from Amomum villosum Lour.


CONCLUSIONS
The ITS-1 sequence can be used to identify effectively Amomum villosum Lour. and their adulterants.
Pan
H
H
Guangzhou University of TCM, Guangzhou 510405.
Huang
F
F
Wang
P
P
Zhou
L
L
Cao
L
L
Liang
R
R
D035801:Amomum / Q000235:genetics*; D001483:Base Sequence; D005638:Fruit / Q000235:genetics; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA