Structure of murine Tcl1 at 2.5 A resolution and implications for the TCL oncogene family.
Tcl1 and Mtcp1, members of the Tcl1 family, are implicated in T-cell prolymphocytic leukemia. The crystal structure of a dimer of murine Tcl1 has been determined at 2.5 A resolution with an R factor of 0.225. Murine Tcl1, human Tcl1 and Mtcp1 share very similar subunit structures, with RMS differences of 0.6 and 1.4 A for C(alpha) atoms, respectively, while the sequences share 50 and 36% identity, respectively. These structures fold into an eight-stranded beta-barrel of unique topology and high internal symmetry of 1.1-1.3 A for the two halves of human and murine Tcl1 and 1.7 A for Mtcp1, despite the low 12-13% sequence identity. The molecular surfaces of all three structures showed a common planar region which is likely to be involved in protein-protein interactions.
Petock
J M
JM
Department of Biology, Georgia State University, Atlanta, GA 30303, USA.
Torshin
I Y
IY
Wang
Y F
YF
Du Bois
G C
GC
Croce
C M
CM
Harrison
R W
RW
Weber
I T
IT
D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000502:physiology; D020558:GTP Phosphohydrolases / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011518:Proto-Oncogene Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000737:chemistry* / Q000502:physiology; D020741:rho GTP-Binding Proteins
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; C485944:Rhoj protein, mouse; C490393:Tcl1 protein, mouse; D014157:Transcription Factors; D020558:GTP Phosphohydrolases; D020741:rho GTP-Binding Proteins