Chromosome instability induced in the cell progeny of human T lymphocytes irradiated in G(0) with gamma-rays.
We report the occurrence of chromosome instability in human T lymphocytes irradiated in vitro with gamma-rays and cultured for several generations before analysis. The delayed effects of gamma-radiation have been evaluated by conventional and molecular (chromosome painting) cytogenetics in preparations obtained from long-term bulk cultures or clonal cultures. The results indicate that the cell progeny of gamma irradiated human T lymphocytes can be characterized by a higher rate of chromosome damage, but this effect depends on the individual donor response to ionizing radiation. Evidence has been collected about a differential involvement of chromosomes 7, 9 and 19 in the induced chromosome rearrangements, and this effect is equally visible as an immediate or delayed response of human T lymphocytes to ionizing radiation.
Bortoletto
E
E
Department of Biology, University of Padova, Via U. Bassi 58/B, 35020 Legnaro, Padova, Italy.
Mognato
M
M
Ferraro
P
P
Canova
S
S
Cherubini
R
R
Celotti
L
L
Russo
A
A
D000328:Adult; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002999:Clone Cells / Q000528:radiation effects; D020732:Cytogenetic Analysis; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008297:Male; D016192:Resting Phase, Cell Cycle / Q000235:genetics / Q000528:radiation effects*; D013601:T-Lymphocytes / Q000166:cytology / Q000528:radiation effects*