Thiosulphate improves yield of hydrogen production from glucose by the immobilized formate hydrogenlyase system of Escherichia coli.
Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen production but reduced the consumption of glucose for the same amount of hydrogen produced compared to control. Oxaloacetate and traces of pyruvate instead of succinate accumulated at the end when thiosulphate was present.
Nandi
R
R
Department of Applied Biochemistry, Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata 700 032, India.
Dey
S
S
Sengupta
S
S
D000693:Anaerobiosis; D004579:Electron Transport; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000378:metabolism*; D062907:Oxaloacetic Acid / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism*
D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D013885:Thiosulfates; D062907:Oxaloacetic Acid; D006859:Hydrogen; D019289:Pyruvic Acid; D006864:Hydrogenase; D005560:Formate Dehydrogenases; C054017:formate hydrogenlyase; D005947:Glucose