title: Isolation and partial characterization of Het- Fix- mutant strain of the diazotrophic cyanobacterium Anabaena variabilis showing chromatic adaptation.
abstract: We propose a model to describe the changes taking place in biochemical processes/events to explain the development of heterocyst and nitrogenase in a diazotrophic cyanobacterium Anabaena variabilis. For this purpose, a mutant strain of A. variabilis lacking heterocyst differentiation and incapable of growth with dinitrogen as the sole source of nitrogen has been isolated after nitrosoguanidine (NTG) mutagenesis and selection by penicillin enrichment. The mutant strain (Het- Fix-) thus isolated has morphological variation and was incapable of reducing acetylene under anaerobic conditions, indicating its mutational loss of the process of nitrogen fixation. The Het- Fix- mutant strain had reduced glutamine synthetase (transferase) activity compared with its wild-type counterpart, suggesting a link between nif gene expression and the expression of gln A, the structural gene of GS. The Het- Fix- mutant strain compared with its wild-type strain also had an extremely high level of phycobiliprotein and a low level of carotenoids. Furthermore, the coiling of vegetative filaments in the Het- Fix- mutant strain, which reduced the surface area to be exposed to light, was a direct indication of the chromatic adaptation, because the mutant strain was found to be photosensitive, showing bleaching of the cells under high light intensity.
authors.affiliation: Institute of Microbiology and Biotechnology, Barkatullah University, Bhopal, India.
authors.forename: B
authors.initials: B
authors.lastname: Singh
authors.forename: V S
authors.initials: VS
authors.lastname: Chauhan
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: P S
authors.initials: PS
authors.lastname: Bisen
mesh_terms: D017033:Anabaena / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D002338:Carotenoids / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
chemical_list: D045342:Light-Harvesting Protein Complexes; D011506:Proteins; D002338:Carotenoids; D009591:Nitrogenase