Comparative evaluation of pectolytic and proteolytic enzyme production by free and immobilized cells of some strains of the phytopathogenic Erwinia chrysanthemi.
Whole cells of the phytopathogenic Erwinia chrysanthemi strains were immobilized in k-carrageenan and grown in high-calcium Xanthomonas campestris medium containing sodium polypectate as carbon source. All the strains used survived immobilization into k-carrageenan beads. Immobilized E. chrysanthemi strains displayed higher pectolytic and proteolytic enzyme activities than free cells in liquid suspension. Carrageenan immobilization techniques could provide a system to mimic the conditions of E. chrysanthemi cells in the infected plant tissue. This could prompt a thorough study of the factors governing the biosynthesis of virulence factors by this bacterium.
Muyima
N Y
NY
Environmental and Natural Products Biotechnology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, P/B X1314, Alice 5700, South Africa.
Zamxaka
M
M
Mazomba
N T
NT
D002351:Carrageenan; D018914:Cells, Immobilized; D003470:Culture Media; D016974:Dickeya chrysanthemi / Q000145:classification / Q000201:enzymology* / Q000254:growth & development*; D010450:Endopeptidases / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D011133:Polysaccharide-Lyases / Q000378:metabolism*
D003470:Culture Media; D002351:Carrageenan; D010450:Endopeptidases; D011133:Polysaccharide-Lyases; C023911:pectate lyase