Structure-function studies of Escherichia coli biotin synthase via a chemical modification and site-directed mutagenesis approach.
In Escherichia coli, biotin synthase (bioB gene product) catalyzes the key step in the biotin biosynthetic pathway, converting dethiobiotin (DTB) to biotin. Previous studies have demonstrated that BioB is a homodimer and that each monomer contains an iron-sulfur cluster. The purified BioB protein, however, does not catalyze the formation of biotin in a conventional fashion. The sulfur atom in the iron-sulfur cluster or from the cysteine residues in BioB have been suggested to act as the sulfur donor to form the biotin molecule, and yet unidentified factors were also proposed to be required to regenerate the active enzyme. In order to understand the catalytic mechanism of BioB, we employed an approach involving chemical modification and site-directed mutagenesis. The properties of the modified and mutated BioB species were examined, including DTB binding capability, biotin converting activity, and Fe(2+) content. From our studies, four cysteine residues (Cys 53, 57, 60, and 97) were assigned as the ligands of the iron-sulfur cluster, and Cys to Ala mutations completely abolished biotin formation activity. Two other cysteine residues (Cys 128 and 188) were found to be involved mainly in DTB binding. The tryptophan and histidine residues were suggested to be involved in DTB binding and dimer formation, respectively. The present study also reveals that the iron-sulfur cluster with its ligands are the key components in the formation of the DTB binding site. Based on the current results, a refined model for the reaction mechanism of biotin synthase is proposed.
Farh
L
L
Department of Natural Science Education, National Pingtung Teachers College, Pingtung, Taiwan.
Hwang
S Y
SY
Steinrauf
L
L
Chiang
H J
HJ
Shiuan
D
D
D000595:Amino Acid Sequence; D001665:Binding Sites; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D019281:Dimerization; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013466:Sulfurtransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D007506:Iron-Sulfur Proteins; D001710:Biotin; D013466:Sulfurtransferases; C057985:biotin synthetase; D003545:Cysteine