Lactate dehydrogenase activity as an effect criterion in toxicity tests with Daphnia magna straus.
Activity of lactate dehydrogenase (LDH) was used as an effect criterion in toxicity tests with Daphnia magna. In the first part of the work, the conditions for the use of LDH activity in toxicity tests with juveniles and adults of D. magna, were optimized. The influence of parameters such as the number of animals per sample, nutritional status, age and the presence of eggs in the brood chamber were investigated. In the second part of the study, both in vivo and in vitro tests based on the alteration of LDH activity of D. magna were developed and tested using zinc chloride as test substance. The results obtained indicate that LDH activity of D. magna may be used as an indicative parameter in aquatic toxicity tests.
Diamantino
T C
TC
Departamento de Zoologia, Instituto do Ambiente e Vida, Largo Marquês de Pombal, Universidade de Coimbra, Portugal. teresa.diamantino@mail.ineti.pt
Almeida
E
E
Soares
A M
AM
Guilhermino
L
L
D000367:Age Factors; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002712:Chlorides / Q000633:toxicity; D003621:Daphnia / Q000201:enzymology*; D004531:Eggs; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D009067:Mouthwashes / Q000633:toxicity; D009752:Nutritional Status; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D017967:Zinc Compounds / Q000633:toxicity
D015415:Biomarkers; D002712:Chlorides; D009067:Mouthwashes; D014874:Water Pollutants, Chemical; D017967:Zinc Compounds; C016837:zinc chloride; D007770:L-Lactate Dehydrogenase