title: An integron-associated beta-lactamase (IBC-2) from Pseudomonas aeruginosa is a variant of the extended-spectrum beta-lactamase IBC-1.
abstract: An extended-spectrum beta-lactamase, IBC-2, produced by a clinical strain of Pseudomonas aeruginosa, was characterized. bla(IBC-2) was found, as a gene cassette, to be the sole gene within the variable region of a class 1 integron probably located in the chromosome. IBC-2 is a variant of IBC-1 and GES-1, differing by one amino acid from each of these beta-lactamases. When expressed in Escherichia coli, IBC-2 was observed to confer resistance to ceftazidime and decreased susceptibility to other oxyimino-beta-lactams.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Mavroidi
authors.forename: E
authors.initials: E
authors.lastname: Tzelepi
authors.forename: A
authors.initials: A
authors.lastname: Tsakris
authors.forename: V
authors.initials: V
authors.lastname: Miriagou
authors.forename: D
authors.initials: D
authors.lastname: Sofianou
authors.forename: L S
authors.initials: LS
authors.lastname: Tzouvelekis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002442:Ceftazidime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
chemical_list: D002511:Cephalosporins; D004251:DNA Transposable Elements; D002442:Ceftazidime; C405902:GES-1 beta-lactamase; C413407:integron-associated beta-lactamase 1; D001618:beta-Lactamases