title: Isolation and Stereostructures of Dolastatin G and Nordolastatin G, Cytotoxic 35-Membered Cyclodepsipeptides from the Japanese Sea Hare Dolabella auricularia.
abstract: A bioassay-directed fractionation of the cytotoxic constituents of the Japanese sea hare Dolabella auricularia resulted in the isolation of two 35-membered depsipeptides dolastatin G (1) and nordolastatin G (2), which showed cytotoxicity against HeLa S(3) cells with IC(50) values of 1.0 and 5.3 &mgr;g/mL, respectively. The gross structures of these substances were established by spectroscopic analysis including 2D NMR techniques. The absolute stereostructure of 1 was determined by chiral HPLC analysis of amino acid components obtained from acid hydrolysis of 1 and by the enantioselective syntheses of two degradation products arising from polyketide portions. Nordolastatin G (2) is a congener that has the same absolute stereochemistry as that of 1.
authors.affiliation: Department of Chemistry, Faculty of Science, Nagoya University, Chikusa, Nagoya 464, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Mutou
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Makoto
authors.initials: M
authors.lastname: Ojika
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Yamada