title: Vaccine-induced antibodies to the native, oligomeric envelope glycoproteins of primary HIV-1 isolates.
abstract: A simple and sensitive method for measuring antibodies to primary human immunodeficiency virus type 1 (HIV-1) isolates has been developed. The flow cytometric immuno-fluorescence assay detects antibodies that bind to the native, oligomeric form of the envelope glycoprotein (gp120) expressed on the surface of PM-1 cells infected with primary isolates of HIV-1. Sera from people infected with HIV-1 or those immunized with recombinant gp120 vaccines were tested. Significant correlation was observed between neutralizing activity and oligomeric gp120 binding activity. Thirteen to 100% of individuals immunized with the subtype B bivalent vaccine AIDSVAX B/B developed oligomeric gp120 binding antibodies against a variety of subtype B primary isolates. For several isolates, AIDSVAX B/B sera reacted better than monovalent AIDSVAX B sera, suggesting that addition of the second immunogen improved the breadth of the antibody response. Cross-subtype binding activities, induced by AIDSVAX B/B, were lower than activities to subtype B isolates, suggesting that additional immunogen(s) may be desirable in vaccine(s) formulated for geographic regions where non-B subtypes are dominant.
authors.affiliation: VaxGen Inc., 1000 Marina Boulevard, Brisbane, CA 94005-1841, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Orque
authors.forename: P A
authors.initials: PA
authors.lastname: Escarpe
authors.forename: M L
authors.initials: ML
authors.lastname: Peterson
authors.forename: J W
authors.initials: JW
authors.lastname: Good
authors.forename: E M
authors.initials: EM
authors.lastname: Zaharias
authors.forename: P W
authors.initials: PW
authors.lastname: Berman
authors.forename: H W
authors.initials: HW
authors.lastname: Sheppard
authors.forename: R
authors.initials: R
authors.lastname: Shibata
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D002460:Cell Line; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D014614:Vaccines, Synthetic