Prolactin-stimulated polymerization of acetyl-coA carboxylase in explants of mid-pregnant rat mammary gland.
Prolactin significantly increased the rate of fatty acid synthesis in explants of mid-pregnant rat mammary gland cultured for 96 h with insulin plus corticosterone. Under these conditions, prolactin increased the specific activity of total acetyl-CoA carboxylase in nuclear-free homogenates of explants by 2.6, and increased the proportion of the enzyme in the active polymeric form from 0.44 to 0.89. Removal of prolactin after 48 h in culture decreased the specific activity of the total enzyme by about half. and decreased the proportion as polymer to 0.52. The results show that prolactin plays a major role in mid-pregnant rat mammary gland in the polymerization which accompanies increased activity of the total enzyme and increased rate of fatty acid synthesis.
Oben
J E
JE
School of Animal and Microbial Sciences, The University of Reading, Whiteknights, UK.
Dils
R R
RR
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000818:Animals; D046508:Culture Techniques; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D008321:Mammary Glands, Animal / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats
D005227:Fatty Acids; D011388:Prolactin; D000103:Acetyl-CoA Carboxylase