title: Aedes aegypti glutamine synthetase: expression and gene structure.
abstract: The peritrophic matrix (PM) is the first natural barrier a mosquito-borne parasite faces when ingested with a blood meal; consequently, understanding the biology of PM formation could provide novel transmission control strategies. Because the PM is composed of chitin (a molecule of repeating units of N-acetyl glucosamine), glycoproteins and glucose, characterizing the regulation of enzymes involved in chitin production should provide information concerning factors that influence PM formation. We previously have shown that glutamine synthetase (GS) provides the glutamine needed in the initial steps of chitin biosynthesis in the yellow fever mosquito, Aedes aegypti. In the present study we show that GS is encoded by a single 4.5 kb gene, designated mGS, containing three exons and two introns. Multiple transcripts are generated from mGS presumably by differential splicing of the introns. Sequences of two cDNAs encoding GS are identical at the protein level, but differ in their 5'-untranslated regions. GS message is constitutively expressed in all developmental stages and in most tissues, with an increase in GS transcription observed in midgut and fat body tissues of female mosquitoes following a blood meal. Transcripts are localized to the apical side of the mosquito midgut epithelium and data suggest that mGS transcription is regulated by an Oct-1 transcription factor.
authors.affiliation: Department of Animal Health and Biomedical Sciences, University of Wisconsin - Madison, 1656 Linden Drive, Madison, WI 53706, USA.
authors.forename: C T
authors.initials: CT
authors.lastname: Smartt
authors.forename: L M
authors.initials: LM
authors.lastname: Kiley
authors.forename: J F
authors.initials: JF
authors.lastname: Hillyer
authors.forename: R
authors.initials: R
authors.lastname: Dasgupta
authors.forename: B M
authors.initials: BM
authors.lastname: Christensen
mesh_terms: D000330:Aedes / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004064:Digestive System / Q000201:enzymology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes / Q000235:genetics; D005970:Glutamate Synthase / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D005970:Glutamate Synthase