Microbiological assay for determination of ofloxacin injection.
A simple, sensitive and specific agar diffusion bioassay for the antibacterial ofloxacin was developed using a strain of Micrococcus luteus ATCC 9341 as the test organism, ofloxacin at concentration ranging 12-27 microg ml(-1) could be measured in injection. A prospective validation of the method showed that method was linear (r=0.9994) and precise (CV=1.14). UV spectrophotometric and high performance liquid chromatographic techniques were chosen as a comparison methods for the determination of ofloxacin. The results obtained by three methods were statistically analyzed by analysis of variance (ANOVA) and the results obtained indicate that there is no significant difference among these methods.
Ev
Lisiane da Silveira
Lda S
Escola de Farmácia, Universidade Federal de Ouro Preto, Rua Domingos Barroso, 51, Saramenha, CEP 35.400-000, MG, Ouro Preto, Brazil. lisiane@ef.ufop.br
Schapoval
Elfrides E S
EE
D000890:Anti-Infective Agents / Q000032:analysis* / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000187:drug effects; D015242:Ofloxacin / Q000032:analysis* / Q000494:pharmacology; D011786:Quality Control; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D012996:Solutions; D015242:Ofloxacin