title: Segregation of microsatellite alleles and residual heterozygosity at single loci in homozygous androgenetic common carp (Cyprinus carpio L.).
abstract: Thirty-three androgenetic progeny groups of common carp were analysed using 11 microsatellite markers to (i) verify the homozygous status of the 566 androgenetic individuals, (ii) analyse the microsatellite allele segregation, and (iii) study the possible association of microsatellite alleles with phenotypic traits. In total, 92% of the androgenetic individuals proved to be homozygous at all 11 loci. Forty-three of the 47 heterozygous individuals were heterozygous at a single locus only. This heterozygosity was probably due to DNA fragments caused by UV irradiation of the eggs. although the maternal origin of the fragments could not be proved beyond doubt. Screening with 11 microsatellites also revealed two linkage groups, a segregation distortion at two microsatellite loci, and the possible association of some microsatellites with mass, length, stress-related plasma cortisol levels, and basal plasma glucose levels. The success of the linkage and association study could be explained by a low recombination frequency due to high chiasma interference. This would imply a relatively short genetic map for common carp.
authors.affiliation: Institute of Animal Sciences, Wageningen University, The Netherlands.
authors.forename: M W
authors.initials: MW
authors.lastname: Tanck
authors.forename: A P
authors.initials: AP
authors.lastname: Palstra
authors.forename: M
authors.initials: M
authors.lastname: van der Weerd
authors.forename: C P
authors.initials: CP
authors.lastname: Leffering
authors.forename: J J
authors.initials: JJ
authors.lastname: van der Poel
authors.forename: H
authors.initials: H
authors.lastname: Bovenhuis
authors.forename: J
authors.initials: J
authors.lastname: Komen
mesh_terms: D000483:Alleles; D000818:Animals; D002347:Carps / Q000235:genetics*; D020090:Chromosome Segregation; D005260:Female; D008040:Genetic Linkage; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D010641:Phenotype; D019655:Quantitative Trait, Heritable