title: Degradation of 1,3-dichloropropene by a soil bacterial consortium and Rhodococcus sp. AS2C isolated from the consortium.
abstract: A bacterial consortium capable of degrading the fumigant 1,3-D ((Z)- and (E)- 1,3-dichloropropene) was enriched from an enhanced soil. This mixed culture degraded (Z)- and (E)-1,3-D only in the presence of a suitable biodegradable organic substrate, such as tryptone, tryptophan, or alanine. After 8 months of subculturing at 2- to 3-week intervals, a strain of Rhodococcus sp. (AS2C) that was capable of degrading 1,3-D cometabolically in the presence of a suitable second substrate was isolated. (Z)-3-chloroallyl alcohol (3-CAA) and (Z)-3-chloroacrylic acid (3-CAAC), and (E)-3-CAA and (E)-3-CAAC were the metabolites of (Z)- and (E)- 1,3-D, respectively. (E)- 1,3-D was degraded faster than (Z)- 1,3-D by the strain AS2C and the consortium. AS2C also degraded (E)-3-CAA faster than (Z)-3-CAA. Isomerization of (E)- 1,3-D to (Z)- 1,3-D or the (Z) form to the (E) form did not occur.
authors.affiliation: Soil and Water Science Department, University of Florida, Gainesville 32611-0290, USA. lto@gnv.ifas.ufl.edu
authors.forename: L T
authors.initials: LT
authors.lastname: Ou
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas
authors.forename: K Y
authors.initials: KY
authors.lastname: Chung
authors.forename: A V
authors.initials: AV
authors.lastname: Ogram
mesh_terms: D000498:Allyl Compounds / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D020005:Propanols / Q000737:chemistry / Q000378:metabolism; D012240:Rhodococcus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D000498:Allyl Compounds; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D020005:Propanols; D012989:Soil Pollutants; C035825:3-chloroallyl alcohol; C023891:1,3-dichloro-1-propene