Microsized 2D protein arrays immobilized by micro-stamps and micro-wells for disease diagnosis and drug screening.
A novel method of protein array immobilization, using micro stamps to pick up proteins from micro wells and deposit them on to a bio-absorption chip, has been developed. This method can potentially transfer several protein spots on to an organized array for applications such as disease diagnosis and drug screening by parallel biological or chemical processes. Fabrication of the micro stamp and the micro well arrays involves thick-photoresist lithography, bulk micromachining, and a molding process, whereas fabrication of the bio-absorption chip involves amino-modification by use of APTS (aminopropyItrimethoxysilane) and surface activation by use of BS3 (bis-sulfosuccinimidyl suberate). Successful transfer of protein on to the bio-absorption surface using the micro stamp-well array has been demonstrated. The size variation between different stamping spots has been shown to be less than 10%, and the APTS-BS3 surface has also been proved to bind the protein efficiently. Appreciable protein retention was achieved during 6-h washing, which shows the binding strength of the bio-absorption surface is sufficient for protein processing.
Lin
S C
SC
Engineering and System Science Dept, National Tsing Hua University, Taiwan, ROC.
Tseng
F G
FG
Huang
H M
HM
Huang
C Y
CY
Chieng
C C
CC
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D011506:Proteins; D010047:Ovalbumin