title: Overexpression of laminin alpha1 chain in colonic cancer cells induces an increase in tumor growth.
abstract: Laminins represent a growing family of glycoproteins constituting the basement membrane. They are known to direct many biological processes. With respect to carcinogenesis, laminins play an important role in cell adhesion, mitogenesis, differentiation and even metastasis. To further study the biological significance of laminin-1 (composed of alpha1, beta1 and gamma1 chains) in intestinal cell differentiation or tumorigenesis, an alpha1-laminin expression vector was introduced into the HT29 colonic cancer cells, in which laminin alpha1 chain is not expressed. Upon transfection of the alpha1 chain, the alpha1beta1gamma1 trimer was found secreted in the media along with free alpha1 chain as assessed by immunoprecipitation. The presence of the laminin alpha1 chain did not significantly modify the levels of the other laminin chains nor the integrins expressed by the HT29 cells. In spite of similar growth properties with the control cells in vitro (plastic dish, soft agar), the laminin alpha1 transfectants showed a significantly increased tumor growth when injected in nude mice. Histologic and immunohistochemic examination of the laminin alpha1-expressing tumors points to an increased recruitment of the host stromal and vascular cells, without modification in the differentiation profile and invasion potential. In parallel, a clear accumulation of laminin-10 (alpha5beta1gamma1) at the carcinoma/stromal interface and a segregation of the integrin beta4 subunit at the basal pole of the cancer cells occurred, compared to control tumors. Overall, our observations emphasize the importance of laminin-1 as a chemoattractant of both stromal and vascular cells and in epithelial/stromal cell interactions for the organization of the basement membrane and segregation of integrins leading to an epithelial cell growth signal. Such a sequence of events is reminiscent of what occurs during development.
authors.affiliation: INSERM Unité 381, Ontogénèse et Pathologie du Système Digestif, 3 Avenue Molière, 67200 Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: De Arcangelis
authors.forename: O
authors.initials: O
authors.lastname: Lefebvre
authors.forename: A
authors.initials: A
authors.lastname: Méchine-Neuville
authors.forename: C
authors.initials: C
authors.lastname: Arnold
authors.forename: A
authors.initials: A
authors.lastname: Klein
authors.forename: L
authors.initials: L
authors.lastname: Rémy
authors.forename: M
authors.initials: M
authors.lastname: Kedinger
authors.forename: P
authors.initials: P
authors.lastname: Simon-Assmann
mesh_terms: D000818:Animals; D002455:Cell Division; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D019073:HT29 Cells; D006801:Humans; D007797:Laminin / Q000032:analysis / Q000502:physiology*; D051379:Mice; D009368:Neoplasm Transplantation; D011817:Rabbits; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D007797:Laminin; C084098:laminin A