title: Cytotoxic quaternary alkaloids from the flowers of Narcissus tazetta.
abstract: Intensive chromatographic separation of the polar fraction of an ethanolic extract of the fresh flowers of Narcissus tazetta L. (Amaryllidaceae) yielded two new quaternary alkaloids with a phenanthrene skeleton, N-methyl-8,9-methylenedioxy-phenantridinium methylsulfate (1) and N-methyl-8,9-methylenedioxy-phenantridinium malate (2). The structure determination of the alkaloids was based on one- and two-dimensional NMR studies including HMQC, and HMBC studies, and mass spectroscopic analysis. The existence, in 1, of the methylsulfate group was confirmed by X-ray diffraction analysis. Cytotoxic activities for 1 and 2 against a panel of cancer cell lines are also reported.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt. youssefdiaa@hotmail.com
authors.forename: D T
authors.initials: DT
authors.lastname: Youssef
authors.forename: A A
authors.initials: AA
authors.lastname: Khalifa
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D011522:Protons; D012996:Solutions
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D007202:Indicators and Reagents; D011522:Protons; D012996:Solutions