Detection of the genotype of apolipoprotein H purified from the serum of a Chinese subject by capillary isoelectric focusing electrophoresis.
Capillary isoelectric focusing electrophoresis (cIEF) has been developed to detect the genotype of apolipoprotein H, which was purified from the serum of a Chinese subject. Depending upon the observation of splitting peaks in cIEF, it is possible to determine if the protein was expressed from a heterozygote gene.
Ru
Q H
QH
School of Life Science and Engineering, Tsinghua University Beijing, People's Republic of China.
Luo
G A
GA
Sui
S F
SF
Wang
S X
SX
D001797:Blood Protein Electrophoresis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006580:Genetic Carrier Screening / Q000379:methods*; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D053482:beta 2-Glycoprotein I
D006023:Glycoproteins; D053482:beta 2-Glycoprotein I