title: Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation.
abstract: Annexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached low levels thereafter. However, Anx 5 and 6 rates were more constant. Prenatal administration of dexamethasone (dex) resulted in a decrease of Anx 1 mRNA levels, and a strong increase in Anx 2 mRNA contents. In adult hepatocytes cultured in the presence of EGF or HGF, Anx 1 and 2 expressions resumed. By immunohistochemistry, Anx 1 was detected only in the cytoplasm of hepatocytes of 1- to 3-day-old rats, Anx 2 and 6 both exhibited a redistribution from the cytoplasm toward the plasma membrane, and Anx 5 was present in the nucleus, cytoplasm, and plasma membrane. Thus, Anx 1, 2, 5, and 6 have individual modes of expression and localization in the differentiating hepatocytes, where they might play unique roles at well defined phases of liver ontogeny.
authors.affiliation: UFR Biomédicale des Saints-Pères, Université René Descartes, 45 rue des Saints-Pères, 75270 Paris cedex 06, France.
authors.forename: B
authors.initials: B
authors.lastname: Della Gaspera
authors.forename: F
authors.initials: F
authors.lastname: Braut-Boucher
authors.forename: M
authors.initials: M
authors.lastname: Bomsel
authors.forename: F
authors.initials: F
authors.lastname: Chatelet
authors.forename: C
authors.initials: C
authors.lastname: Guguen-Guillouzo
authors.forename: J
authors.initials: J
authors.lastname: Font
authors.forename: J
authors.initials: J
authors.lastname: Weinman
authors.forename: S
authors.initials: S
authors.lastname: Weinman
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000235:genetics; D017306:Annexin A2 / Q000235:genetics; D017304:Annexin A5 / Q000235:genetics; D017317:Annexin A6 / Q000235:genetics; D017302:Annexins / Q000235:genetics*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D005938:Glucocorticoids / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000166:cytology / Q000196:embryology*; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017305:Annexin A1; D017306:Annexin A2; D017304:Annexin A5; D017317:Annexin A6; D017302:Annexins; D005938:Glucocorticoids; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D003907:Dexamethasone