Factor Xa (FXa) inhibitor from the nymphs of the camel tick Hyalomma dromedarii.
An inhibitor of factor Xa (FXa) was isolated from the nymphs of the camel tick Hyalomma dromedarii by a combination of chromatography on DEAE-cellulose and Sephacryl S-300 columns. The isolated nymphal FXa inhibitor turned out to be a homogenous preparation of a single polypeptide chain (15 kDa) as judged by both the native and denatured SDS-PAGE. Its pI value ranged from 7.7 to 7.9. The inhibitor is a potent anticoagulant since it prolonged both the activated partial thromboplastin time (APTT) and the prothrombin time (PT) of the camel plasma in a concentration-dependent manner. Its activity was threefold lower toward thrombin than FXa, but it did not inhibit any of the proteases; trypsin, alpha-chymotrypsin, papain, pepsin and subtilisin. The inhibitor binds at two sites on FXa uncompetitively with an inhibition constant (K(i)) value of 134 nM.
Ibrahim
M A
MA
Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Cairo, Egypt. ibrahimm70@hotmail.com
Ghazy
A H
AH
Maharem
T M
TM
Khalil
M I
MI
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D001665:Binding Sites; D001667:Binding, Competitive; D002162:Camelus; D002848:Chromatography, DEAE-Cellulose; D002918:Chymotrypsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D065427:Factor Xa Inhibitors; D018884:Ixodes; D007700:Kinetics; D009758:Nymph; D010206:Papain / Q000494:pharmacology; D010314:Partial Thromboplastin Time; D010434:Pepsin A / Q000494:pharmacology; D011485:Protein Binding; D011516:Prothrombin / Q000378:metabolism; D020860:Subtilisin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D000925:Anticoagulants; D065427:Factor Xa Inhibitors; D011516:Prothrombin; D002918:Chymotrypsin; C117608:alpha-chymotrypsin; D014357:Trypsin; D020860:Subtilisin; D010206:Papain; D010434:Pepsin A