Cell cycle-dependent protein secretion by Saccharomyces cerevisiae.
Synchronized Saccharomyces cerevisiae cell populations were used to examine secretion rates of a heterologous protein as a function of cell cycle position. The synchronization procedure had a profound effect on the type and quality of data obtained. When cell synchrony was induced by cell cycle-arresting drugs, a significant physiological perturbation of cells was observed that obscured representative secretion data. In contrast, synchronization with centrifugal elutriation resulted in synchronized first-generation daughter cells with undetectable perturbation of the physiological state. The synchronized cells did not secrete significant amounts of protein until they reached cell division, suggesting that the secretion process in these cells is strongly cell cycle dependent. However, the maximum secretion rate of the synchronized culture (7-14 molecules/cell/second) was significantly lower than that of an asynchronous culture (29-51 molecules/cell/second). This result indicates that young daughter cells isolated in the synchronization process exhibit different protein secretion behavior than older mother cells that are absent in the synchronized cell population but present in the asynchronous culture.
Frykman
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S
Department of Chemical Engineering and Materials Science, 151 Amundson Hall, 421 Washington Ave. S.E., Minneapolis, MN 55455-0132, USA.
Srienc
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F
D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D018797:Cell Cycle Proteins; D003470:Culture Media; D007377:Interleukin-3; D016178:Granulocyte-Macrophage Colony-Stimulating Factor