Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis.
Protein phosphorylation and dephosphorylation are key regulatory mechanisms in prokaryotic and eukaryotic cells. Considering the role of phosphorylation in many human diseases, it appears a major challenge to refine on the methods to analyze the phospho-proteome. Here we review the use of monoclonal antibodies directed against specific phosphorylated amino acid residues to visualize phosphoproteins separated by two-dimensional gel electrophoresis. Strategies are described how this method can successfully be applied to create phospho-proteome maps of mammalian cells.
Kaufmann
H
H
Institute of Biotechnology, Zurich, Switzerland.
Bailey
J E
JE
Fussenegger
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D000939:Epitopes; D006801:Humans; D010750:Phosphoproteins / Q000276:immunology* / Q000302:isolation & purification*; D010766:Phosphorylation; D020543:Proteome
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010750:Phosphoproteins; D020543:Proteome