An invertebrate defense molecule activates membrane conductance in mammalian cells by means of its lectin-like domain.
The invertebrate defense molecule Coelomic Cytolytic Factor-1 (CCF-1) and the mammalian cytokine Tumor Necrosis Factor (TNF) share a similar lectin-like domain that, upon interaction with specific sugars, causes lysis of African trypanosomes. In contrast to TNF, CCF-1 does not require an acidification of a lysosomal compartment for this activity. Moreover, we could demonstrate using the whole cell patch clamp technique that both TNF and CCF-1 activate amiloride-sensitive channels in mammalian cells, in a TNF receptor-independent way, but, unlike TNF, CCF-1 does not require acidic conditions for this activity. These data confirm the functional analogies of an invertebrate defense molecule and a mammalian cytokine, based on a similar lectin-like interaction.
Bloc
Alain
A
Department of Pharmacology, Faculty of Medecine, Centre Médical Universitaire, 4, Geneva, Switzerland.
Lucas
Rudolf
R
Van Dijck
Els
E
Bilej
Martin
M
Dunant
Yves
Y
De Baetselier
Patrick
P
Beschin
Alain
A
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D003603:Cytotoxins / Q000494:pharmacology*; D004187:Disaccharides; D004553:Electric Conductivity; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D003603:Cytotoxins; D004187:Disaccharides; D037102:Lectins; D014344:Trypanocidal Agents; D014409:Tumor Necrosis Factor-alpha; C094795:coelomic cytolytic factor 1, Eisenia fetida; D000643:Ammonium Chloride; C010913:N,N-diacetylchitobiose