title: High-throughput PCR in silicon based microchamber array.
abstract: Highly integrated hybridization assay and capillary electrophoresis have improved the throughput of DNA analysis. The shift to high throughput analysis requires a high speed DNA amplification system, and several rapid PCR systems have been developed. In these thermal cyclers, the temperature was controlled by effective methodology instead of a large heating/cooling block preventing rapid thermal cycling. In our research, high speed PCR was performed using a silicon-based microchamber array and three heat blocks. The highly integrated microchamber array was fabricated by semiconductor microfabrication techniques. The temperature of the PCR microchamber was controlled by alternating between three heat blocks of different temperature. In general, silicon has excellent thermal conductivity, and the heat capacity is small in the miniaturized sample volume. Hence, the heating/cooling rate was rapid, approximately 16 degrees C/s. The rapid PCR was therefore completed in 18 min for 40 cycles. The thermal cycle time was reduced to 1/10 of a commercial PCR instrument (Model 9600, PE Applied Biosystems-3 h).
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai Tatsunokuchi, Ishikawa 923-1292, Japan. hnagai@jaist.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: E
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D006358:Hot Temperature; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012666:Semiconductors; D012825:Silicon; D013997:Time Factors
chemical_list: D012825:Silicon