title: The in-vitro influence of serum amyloid A isoforms on enzymes that regulate the balance between esterified and un-esterified cholesterol.
abstract: The intracellular balance between un-esterified and esterified cholesterol is regulated by two enzyme activities, cholesterol ester hydrolases, which drive the balance in favor of un-esterified cholesterol, and acyl-CoA:cholesterol acyl transferase (ACAT) which acts in the opposite direction. During acute inflammation apo-serum amyloid A (apoSAA) isoforms 1.1 and 2.1 become major constituents of high density lipoprotein and this complex is internalized by macrophages. Mixtures of the two isoforms have been shown to enhance cholesterol esterase activity. Using a purified form of the pancreatic enzyme we have explored the mechanism by which apoSAA may accomplish this stimulation. The pancreatic esterase cleaves cholesteryl-oleate with a Km of 0.255 mM, releasing both cholesterol and oleate. Cholesterol exhibits a product inhibition which is relieved by isoform 2.1 but not 1.1 nor apolipoprotein A-I. The NH2-terminal 16 residues of isoform 2.1 had no effect on the esterase, but the 80 residue peptide constituting its COOH-terminus possessed the stimulatory property. Purified isoforms 1.1, 2.1, 2.2, apolipoprotein A-I, the NH2-terminal 16 residues and COOH-terminal 80 residues of isoform 2.1 were also examined for their effects on macrophage ACAT activity. Isoforms 2.1 and 2.2 produced dose dependent inhibitions of up to 50%, (p<0.001). Isoform 1.1, and apoA-I had no effect on ACAT activity. The NH2-terminal 16 residue peptide of isoform 2.1 reduced the ACAT activity in a dose dependent manner by 74% (p<0.001), whereas the COOH-terminal 80 residues, in contrast to its enhancing effect on the esterase, had no inhibitory effect on ACAT. Such complementary but opposite effects of isoform 2.1 on ACAT and the esterase are consistent with a role for this protein in shifting the balance between unesterified (transportable) and esterified (storage) forms of cholesterol in favor of the latter. They suggest that apoSAA2.1 may mediate cholesterol mobilization at sites of tissue injury.
authors.affiliation: Department of Pathology, Queen's University, Kingston General Hospital, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Ely
authors.forename: R
authors.initials: R
authors.lastname: Bonatesta
authors.forename: J B
authors.initials: JB
authors.lastname: Ancsin
authors.forename: M
authors.initials: M
authors.lastname: Kindy
authors.forename: R
authors.initials: R
authors.lastname: Kisilevsky
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000494:pharmacology*; D001059:Apoproteins / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D004951:Esterification; D007249:Inflammation / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000494:pharmacology; D020033:Protein Isoforms / Q000494:pharmacology; D000685:Serum Amyloid A Protein / Q000494:pharmacology*; D002787:Sterol Esterase / Q000187:drug effects / Q000378:metabolism*; D002785:Sterol O-Acyltransferase / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
chemical_list: D001053:Apolipoproteins; D001059:Apoproteins; D010446:Peptide Fragments; D020033:Protein Isoforms; D000685:Serum Amyloid A Protein; D002784:Cholesterol; D002785:Sterol O-Acyltransferase; D002787:Sterol Esterase