Mapping peroxidase in plant tissues by scanning electrochemical microscopy.
Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery (Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H(2)O(2) to increase FMA(+) concentration. Two-dimensional reduction current profiles for FMA(+) produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles.
Zhou
H
H
Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry, Yamagata 990-2473, Japan.
Shiku
H
H
Kasai
S
S
Noda
H
H
Matsue
T
T
Ohya-Nishiguchi
H
H
Kamada
H
H
D000077102:Apium / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000378:metabolism; D008839:Microelectrodes; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D015335:Molecular Probes / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism*; D059828:Plant Cells; D010944:Plants / Q000201:enzymology* / Q000648:ultrastructure; D014018:Tissue Distribution
D005296:Ferrous Compounds; D015335:Molecular Probes; C071550:ferrocenemethanol; D009195:Peroxidase