Effect of recombinant human granulocyte/macrophage colony-stimulating factor on the healing of colonic anastomosis in rats.
The purpose of this study was to investigate the efficacy of recombinant human granulocyte/macrophage colony-stimulating factor (rHuGM-CSF) on the wound healing of colonic anastomosis in rats. In total, 40 male Wistar rats were taken into this study. The control group (n = 20) received subcutaneous saline injection. The experiment group (n = 20) received rHuGM-CSF at 100 microg/kg subcutaneously. Both groups underwent colonic anastomosis 2 days later. In each group, burst pressure and hydroxyproline levels were determined 3 and 7 days after anastomosis. White blood cell (WBC) and red blood cell (RBC) counts were determined in each group to evaluate the effect of rHuGM-CSF on hematologic parameters in rats. There was no significant difference between the two groups in regard to burst pressure and hydroxyproline levels (p > .05). As expected, WBC counts significantly increased in the experiment group on days 3 (p = .035) and 7 (p = .046) following surgery. In conclusion, rHuGM-CSF administration and increased WBC counts did not have any positive effect on the wound healing of colonic anastomosis.
Demirer
S
S
Department of Surgery, Ankara Medical School, Turkey. sdemirer@hotmail.com
Sengül
N
N
Inan
A
A
Eroğlu
A
A
Bumin
C
C
Kuterdem
E
E
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000737:chemistry / Q000502:physiology* / Q000601:surgery*; D004906:Erythrocyte Count; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006909:Hydroxyproline / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006909:Hydroxyproline