Interaction of caveolin with 7-ketocholesterol.
Caveolin is an integral membrane protein that interacts with cholesterol in glycosphingolipid-rich rafts at the cell surface. We have examined the interaction of recombinant His-tagged caveolin-1 with cholesterol and 7-keto cholesterol, the most abundant non-enzymatically formed oxysterol found in oxidised LDL and atheromatous plaque. Our data show that caveolin-1 is able to interact with both sterols. This might have consequences for sterol transport and the signalling properties of cells during atherosclerosis.
Sleer
L S
LS
Heart Research Institute, 145 Missenden Road, NSW 2050, Camperdown, Australia.
Brown
A J
AJ
Stanley
K K
KK
D015153:Blotting, Western; D051242:Caveolin 1; D022461:Caveolins / Q000737:chemistry*; D002499:Centrifugation, Density Gradient; D002784:Cholesterol / Q000737:chemistry; D007653:Ketocholesterols / Q000737:chemistry*; D008081:Liposomes; D011994:Recombinant Proteins / Q000737:chemistry
D051242:Caveolin 1; D022461:Caveolins; D007653:Ketocholesterols; D008081:Liposomes; D011994:Recombinant Proteins; D002784:Cholesterol; C003001:7-ketocholesterol