Uptake of Malassezia furfur by human dermal fibroblasts: effect of ketoconazole and cytoskeleton inhibitors.
We showed the ability of human dermal fibroblasts to take up Malassezia furfur and the effect of ketoconazole and cytoskeleton inhibitors, including cytochalasin D and colchicine, on invasivity. Engulfment was evaluated by May Grunwald Giemsa stain and confirmed by acridine orange staining and electron microscopy. Both revealed the different steps of engulfment, including a fusion event between lysosomes and phagosomes containing M. furfur. Subinhibitory concentrations of ketoconazole (5 microg/ml) reduced the invasive capacity compared to controls (52.0+/-6.3 vs 10.0+/-1.2). M. furfur induced changes in the cytoskeleton of human dermal fibroblasts, with signs of disaggregation of actin fibres. We also studied the effect of the cytoskeleton inhibitors, cytochalasin D (1 microg/ml) and colchicine (1 microg/ml), on engulfment. Cytochalasin D, an inhibitor of actin polymers, inhibited the uptake of M. furfur by human dermal fibroblasts. Colchicine, a microtubule inhibitor, reduced the uptake of M. furfur less markedly. This suggests that the process of engulfment is F-actin-dependent, but the integrity of microtubules is also important in "non-professional" phagocytic cells such as dermal fibroblasts.
Baroni
A
A
Dipartimento di Dermatologia, Facoltà di Medicina e Chirurgia II Università degli Studi di Napoli, Italy.
Perfetto
B
B
Paoletti
I
I
De Martino
L
L
Buommino
E
E
Ruocco
E
E
Ruocco
V
V
D000935:Antifungal Agents / Q000494:pharmacology; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D005347:Fibroblasts / Q000382:microbiology* / Q000502:physiology*; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology; D008290:Malassezia / Q000502:physiology*; D008854:Microscopy, Electron; D009181:Mycoses / Q000382:microbiology*; D012867:Skin / Q000166:cytology / Q000382:microbiology*; D012879:Skin Physiological Phenomena
D000935:Antifungal Agents; D015638:Cytochalasin D; D007654:Ketoconazole; D003078:Colchicine