title: Construction and characterisation of infectious recombinant HIV-1 clones containing CTL epitopes from structural proteins in Nef.
abstract: In this study the construction is described of HIV-1 molecular clones in which CTL epitopes from RT or Env late proteins were inserted into the Nef early protein. The ectopic epitopes were efficiently processed from the recombinant Nef proteins, were recognized by their cognate CTL in cytolytic assays, and did not perturb virus replication or viral protein expression in vitro. These recombinant viruses will therefore be an important tool in studying the effect of distinct epitope expression kinetics on the efficiency of CTL-mediated suppression of HIV-1 replication.
authors.affiliation: Department of Virology, Erasmus Medical Center Rotterdam, PO Box 1738, 3000 DR, Rotterdam, The Netherlands.
authors.forename: Christophe
authors.initials: C
authors.lastname: Guillon
authors.forename: Carel A
authors.initials: CA
authors.lastname: van Baalen
authors.forename: Patrick H M
authors.initials: PH
authors.lastname: Boers
authors.forename: Esther J
authors.initials: EJ
authors.lastname: Verschuren
authors.forename: Rob A
authors.initials: RA
authors.lastname: Gruters
authors.forename: Albert D M E
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology; D015693:Gene Products, nef / Q000235:genetics* / Q000276:immunology; D054303:HIV Reverse Transcriptase / Q000235:genetics / Q000276:immunology; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014779:Virus Replication; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D018984:Epitopes, T-Lymphocyte; D015686:Gene Products, env; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus; D054303:HIV Reverse Transcriptase