In vitro susceptibilities of Bartonella and Rickettsia spp. to fluoroquinolone antibiotics as determined by immunofluorescent antibody analysis of infected Vero cell monolayers.
The in vitro susceptibilities of Bartonella and Rickettsia spp. to different concentrations of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin in Vero cell cultures, were determined by enumeration of immunofluorescent-stained bacilli. After incubation in a CO(2)-enriched atmosphere, inocula were replaced and tested with media containing 12 different concentrations of each antibiotic in replicate for each species and the monolayers were re-incubated. Growth status was determined by evaluation of immunofluorescent staining bacilli. Effective inhibitory antibiotic dilution endpoints were determined by counting Bartonella- and Rickettsia-specific fluorescent foci across a range of antibiotic dilutions with an epi-fluorescent microscope, and were compared with an antibiotic-negative control. Based upon the use of C(max):MIC and AUC:MIC data, levofloxacin exhibited activity against Bartonella elizabethae and B. quintana.
Ives
T J
TJ
School of Pharmacy, Campus Box 7595, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7595, USA. tjives@med.unc.edu
Marston
E L
EL
Regnery
R L
RL
Butts
J D
JD
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001473:Bartonella / Q000187:drug effects*; D002522:Chlorocebus aethiops; D015169:Colony Count, Microbial; D005455:Fluorescent Antibody Technique; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D012281:Rickettsia / Q000187:drug effects*; D014709:Vero Cells / Q000382:microbiology
D000890:Anti-Infective Agents; D024841:Fluoroquinolones