Effect of Mentha x piperita essential oil and monoterpenes on cucumber root membrane potential.
Peppermint (Mentha piperita L.) essential oil and its main components were assessed for their ability to interfere with plant plasma membrane potentials. Tests were conducted on root segments isolated from etiolated seedlings of cucumber (Cucumis sativus L.). Increasing the concentration of peppermint essential oil from 5 to 50 ppm caused a decrease in membrane potential (Vm) hyperpolarization of 10-3 mV, whereas concentrations from 100 up to 900 ppm caused an increasing depolarization of Vm (from 5 to 110 mV). When tested at 300 ppm, (+)-menthyl acetate, (-)-limonene and 1,8-cineole did not exert any significant effect on V(m), whereas (+)-menthofuran (73 mV), (+)-pulegone (85 mV), (+)-neomenthol (96 mV), (-)-menthol (105 mV) and (-)-menthone (111 mV) showed increased ability to depolarize V(m). A plot of log of octanol-water partition coefficient (K(ow)) against their depolarizing effect showed a significant negative correlation, suggesting that among all monoterpenoids increased membrane depolarization depends on lower K(ow). However, among monoterpene ketones, alcohols and furans, increased membrane depolarization is associated with a decline in water solubility. The possible effect of monoterpenoids on membrane ion fluxes is also discussed, since changes in the bioelectric potential of cells imply changes in the flux of ions across the plasma membrane
Maffei
M
M
Department of Plant Biology, University of Turin, Viale P.A. Mattioli 25, I-10125, Torino, Italy. massimo.maffei@unito.it
Camusso
W
W
Sacco
S
S
D018553:Cucumis sativus / Q000166:cytology / Q000502:physiology*; D000081005:Cyclohexane Monoterpenes; D004305:Dose-Response Relationship, Drug; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D036142:Mentha piperita; D008610:Menthol / Q000031:analogs & derivatives* / Q000494:pharmacology; D039821:Monoterpenes; D009822:Oils, Volatile / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000187:drug effects* / Q000502:physiology; D013729:Terpenes / Q000494:pharmacology*
D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; D013729:Terpenes; D008610:Menthol; C054487:menthofuran; C039648:pulegone