Novel antifungal peptides from Ceylon spinach seeds.
Two novel antifungal peptides, designated alpha- and beta-basrubrins, respectively, were isolated from seeds of the Ceylon spinach Basella rubra. The purification procedure involved saline extraction, (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose and FPLC-gel filtration on Superdex peptide column. alpha- and beta-basrubrins exhibited a molecular weight of 4.3 and 5 kDa, respectively. They inhibited translation in a rabbit reticulocyte system with an IC(50) value of 400 and 100 nM, respectively. alpha- and beta-basrubrin inhibited HIV-1 reverse transcriptase by (79.4 +/- 7.8)% and (54.6 +/- 3.6)%, respectively, at a concentration of 400 microM, and (10.56 +/- 0.92)% and (2.12 +/- 0.81)%, respectively, at a concentration of 40 microM. Both alpha- and beta-basrubrins exerted potent antifungal activity toward Botrytis cinerea, Mycosphaerella arachidicola, and Fusarium oxysporum.
Wang
H
H
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
Ng
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020171:Botrytis / Q000187:drug effects; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005658:Fungi / Q000187:drug effects*; D005670:Fusarium / Q000187:drug effects; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D020128:Inhibitory Concentration 50; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012639:Seeds / Q000737:chemistry*; D018724:Spinacia oleracea / Q000737:chemistry*; D013188:Sri Lanka
D000935:Antifungal Agents; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; D010940:Plant Proteins; C441473:basrubrin proteins, Basella rubra; D054303:HIV Reverse Transcriptase