Isolation of spectrin subunits by reverse-phase high-performance liquid chromatography.
We present a one-step uncomplicated method of separation of spectrin subunits. The method is based on reverse-phase HPLC employing an analytical C4 column. Reverse-phase HPLC combines the steps of dissociation and separation of spectrin subunits. The method can be applied to different spectrin isoforms. It can be used for analytical purposes, as well as for small-scale (<0.4 mg) isolation of spectrin subunits.
Kakhniashvili
D G
DG
Department of Cell Biology and Neuroscience, USA Comprehensive Sickle Cell Center, University of South Alabama, Mobile, Alabama 36688, USA.
Goodman
S R
SR
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D020033:Protein Isoforms / Q000302:isolation & purification; D021122:Protein Subunits; D013049:Spectrin / Q000302:isolation & purification*
D020033:Protein Isoforms; D021122:Protein Subunits; D013049:Spectrin