title: Simultaneous enantioseparation of cis-diltiazem hydrochloride and its metabolite cis-desacetyldiltiazem using high-performance liquid chromatography and capillary electrophoresis.
abstract: Two alternative methods were developed using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) for a simultaneous enantioselective determination of Ca(2+) blocking chiral drug cis-diltiazem hydrochloride (DLT) and its degradation product and metabolite cis-desacetyldiltiazem (Desac-DLT). The methods were compared from the viewpoint of analytical performance. The identification of HPLC peaks were performed using off-line electrospray ionization mass spectrometry. In addition, CE method was evaluated for quantitative determination of minor enantiomeric impurities in pharmacologically active (+)-cis-DLT.
authors.affiliation: Institute of Pharmaceutical Chemistry, University of Münster, Hittorfstr. 58-62, 48149, Münster, Germany.
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
authors.forename: Irma
authors.initials: I
authors.lastname: Kartozia
authors.forename: Gottfried
authors.initials: G
authors.lastname: Blaschke
mesh_terms: D002121:Calcium Channel Blockers / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004110:Diltiazem / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D013058:Mass Spectrometry; D015394:Molecular Structure; D013237:Stereoisomerism
chemical_list: D002121:Calcium Channel Blockers; C037769:deacetyldiltiazem; D004110:Diltiazem