Simultaneous enantioseparation of cis-diltiazem hydrochloride and its metabolite cis-desacetyldiltiazem using high-performance liquid chromatography and capillary electrophoresis.
Two alternative methods were developed using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) for a simultaneous enantioselective determination of Ca(2+) blocking chiral drug cis-diltiazem hydrochloride (DLT) and its degradation product and metabolite cis-desacetyldiltiazem (Desac-DLT). The methods were compared from the viewpoint of analytical performance. The identification of HPLC peaks were performed using off-line electrospray ionization mass spectrometry. In addition, CE method was evaluated for quantitative determination of minor enantiomeric impurities in pharmacologically active (+)-cis-DLT.
Chankvetadze
Bezhan
B
Institute of Pharmaceutical Chemistry, University of Münster, Hittorfstr. 58-62, 48149, Münster, Germany.
Kartozia
Irma
I
Blaschke
Gottfried
G
D002121:Calcium Channel Blockers / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004110:Diltiazem / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D013058:Mass Spectrometry; D015394:Molecular Structure; D013237:Stereoisomerism
D002121:Calcium Channel Blockers; C037769:deacetyldiltiazem; D004110:Diltiazem