Efficient FLP recombination in mouse ES cells and oocytes.
We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.
Schaft
J
J
Gene Expression Program, EMBL, Heidelberg, Germany.
Ashery-Padan
R
R
van der Hoeven
F
F
Gruss
P
P
Stewart
A F
AF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D014162:Transfection
D017931:DNA Primers; D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase