Phosphatidylinositol-specific phospholipase C activity in Lactobacillus rhamnosus with capacity to translocate.
Phosphatidylinositol-specific phospholipase C (PI-PLC) activity was investigated in 25 different lactic acid bacteria (LAB) strains belonging to the genera Lactobacillus, Weisella, and Enterococcus. PI-PLC activity was detected in 44% of the strains studied in culture medium without carbon source. From the PI-PLC positive strains, Lactobacillus rhamnosus ATCC 7469 was selected for translocation studies. Healthy mice were orally administered with a daily dose of 2.0 x 10(9) of viable L. rhamnosus suspension. Viable bacteria were detected in liver and spleen of mice fed with LAB for 7 days. Bacterial colonies isolated from liver were biochemically characterized, and further subjected to randomly amplified polymorphic DNA. Amplification patterns of five strains displayed identical profiles to L. rhamnosus. PI-PLC activity was determined in the strains recovered from liver.
Rodriguez
A V
AV
Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000 Tucumán, Argentina. anavirr@cerela.org.ar
Baigorí
M D
MD
Alvarez
S
S
Castro
G R
GR
Oliver
G
G
D000818:Animals; D018988:Bacterial Translocation; D006801:Humans; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D019936:Probiotics; D019105:Random Amplified Polymorphic DNA Technique; D013154:Spleen / Q000382:microbiology; D010738:Type C Phospholipases / Q000378:metabolism*
D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase