title: Dual function of protein confinement in chaperonin-assisted protein folding.
abstract: The GroEL/GroES chaperonin system mediates the folding of a range of newly synthesized polypeptides in the bacterial cytosol. Using a rapid biotin-streptavidin-based inhibition of chaperonin function, we show that the cage formed by GroEL and its cofactor GroES can have a dual role in promoting folding. First, enclosure of nonnative protein in the GroEL:GroES complex is essential for folding to proceed unimpaired by aggregation. Second, folding inside the cage can be significantly faster than folding in free solution, independently of ATP-driven cycles of GroES binding and release. This suggests that confinement of unfolded protein in the narrow hydrophilic space of the chaperonin cage smoothes the energy landscape for the folding of some proteins, increasing the flux of folding intermediates toward the native state.
authors.affiliation: Department of Cellular Biochemistry, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Brinker
authors.forename: G
authors.initials: G
authors.lastname: Pfeifer
authors.forename: M J
authors.initials: MJ
authors.lastname: Kerner
authors.forename: D J
authors.initials: DJ
authors.lastname: Naylor
authors.forename: F U
authors.initials: FU
authors.lastname: Hartl
authors.forename: M
authors.initials: M
authors.lastname: Hayer-Hartl
mesh_terms: D000818:Animals; D019921:Biotinylation; D002417:Cattle; D018835:Chaperonin 10 / Q000737:chemistry; D018834:Chaperonin 60 / Q000737:chemistry*; D018833:Chaperonins / Q000737:chemistry*; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D008854:Microscopy, Electron; D008954:Models, Biological; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry; D019809:Streptavidin / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry; D013997:Time Factors
chemical_list: D018835:Chaperonin 10; D018834:Chaperonin 60; D019809:Streptavidin; D013884:Thiosulfate Sulfurtransferase; D018833:Chaperonins; D012273:Ribulose-Bisphosphate Carboxylase