Human TSC-22 gene: no association with type 2 diabetes.
OBJECTIVE
In order to elucidate whether or not genetic variations of TSC-22 (TGF [transforming growth factor]-beta-stimulated clone 22), which was originally identified as a TGF-beta-responsive leucine zipper protein in murine osteoblastic cells, are associated with type 2 diabetes, the genomic organization of the human TSC-22 gene was determined and the association between its polymorphisms and type 2 diabetes was examined.


RESULTS
The human TSC-22 gene spans approximately 5 kilobase pairs and is encoded in three exons. Two single nucleotide polymorphisms (SNPs) were identified in the coding region of the first exon, two other SNPs in the first intron, and one SNP in the putative promoter region. There were, however, no significant differences in the frequency of these polymorphisms between patients with type 2 diabetes and non-diabetic control subjects.


CONCLUSIONS
It is unlikely that the TSC-22 gene is a locus responsible for type 2 diabetes.
Sugawara
F
F
Department of Metabolism and Clinical Nutrition, Graduate School of Medicine, Kyoto University.
Yamada
Y
Y
Kuroe
A
A
Someya
Y
Y
Kubota
A
A
Ihara
Y
Y
Takahashi
K
K
Seino
Y
Y
D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012097:Repressor Proteins / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D007328:Insulin; D012097:Repressor Proteins; C099726:TSC22D1 protein, human