Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays.
A new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented. Oxidative polymerization was performed in the presence of template; monomers: 3-aminophenylboronic acid (APBA), 3-thiopheneboronic acid (TBA) and aniline were polymerized in water and the polymers were grafted onto the polystyrene surface of the microplates. It was found that this process results in the creation of synthetic materials with antibody-like binding properties. It was shown that the MIP-coated microplates are particularly useful for assay development. The high stability of the polymers and good reproducibility of the measurements make MIP coating an attractive alternative to conventional antibodies or receptors used in enzyme linked immunosorbent assay (ELISA).
Piletsky
S A
SA
Institute of BioScience and Technology, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK. s.piletsky@cranfield.ac.uk
Piletska
E V
EV
Bossi
A
A
Karim
K
K
Lowe
P
P
Turner
A P
AP
D000906:Antibodies; D001280:Atrazine / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004837:Epinephrine / Q000737:chemistry; D015200:Fluoroimmunoassay / Q000379:methods*; D018716:Molecular Mimicry; D011108:Polymers; D011506:Proteins / Q000737:chemistry
D000906:Antibodies; D011108:Polymers; D011506:Proteins; D001280:Atrazine; D004837:Epinephrine