title: Dual-mode gradient HPLC procedure for the simultaneous determination of chloroquine and proguanil.
abstract: In order to assay the antipaludic capsule of the Service de Santé des Armées (SSA), that contains two antimalarial drugs, i.e. chloroquine sulfate (CQS, cp1) and proguanil hydrochloride (PGH, cp5), a HPLC procedure was developed. A reversed-phase ion-pair high-performance liquid chromatography (HPLC) method with an ultraviolet detection at 254 nm was set up and validated. Elution system includes programming of both organic concentration and flow-rate known as 'dual-mode gradient'. This method allows the simultaneous determination of both active compounds and separation of four process related substances. The method is simple, rapid, selective and accurate, and the precision is good with an inter- and intra-assay of <2%. The sensitivity is particularly suitable for pharmaceutical quality control.
authors.affiliation: Laboratoire de Contrôle de la Pharmacie Centrale des Armées (PCA), Orléans, France.
authors.forename: A
authors.initials: A
authors.lastname: Paci
authors.forename: A-M
authors.initials: AM
authors.lastname: Caire-Maurisier
authors.forename: A
authors.initials: A
authors.lastname: Rieutord
authors.forename: F
authors.initials: F
authors.lastname: Brion
authors.forename: P
authors.initials: P
authors.lastname: Clair
mesh_terms: D000962:Antimalarials / Q000032:analysis*; D002738:Chloroquine / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004304:Dosage Forms; D004355:Drug Stability; D002727:Proguanil / Q000032:analysis* / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000962:Antimalarials; D004304:Dosage Forms; D002738:Chloroquine; D002727:Proguanil