Role of C-terminal region of HA-33 component of botulinum toxin in hemagglutination.
Using SDS-PAGE, we found that one subcomponent, hemagglutinin (HA-33), from the Clostridium botulinum progenitor toxin of type D strain 1873 and type C strain Yoichi had slightly smaller molecular sizes than those of type C and D reference strains, but other components did not. Based on N- and C-terminal sequence analyses of HA-33, a deletion of 31 amino acid residues from the C-terminus at a specific site was observed in the HA-33 proteins of both strains. The progenitor toxins from both strains showed poor hemagglutination activities, titers of 2(1) or less, which were much lower than titers from the reference strains (2(6)), and did not bind to erythrocytes. These results suggest strongly that the short C-terminal region of the HA-33 plays an essential role in the hemagglutination activity of the botulinum progenitor toxin. Additionally, a sequence motif search predicted that the C-terminal region of HA-33 has a carbohydrate-recognition subdomain.
Sagane
Y
Y
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, 099-2493, Japan.
Kouguchi
H
H
Watanabe
T
T
Sunagawa
H
H
Inoue
K
K
Fujinaga
Y
Y
Oguma
K
K
Ohyama
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001665:Binding Sites; D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003014:Clostridium botulinum / Q000737:chemistry*; D004912:Erythrocytes / Q000378:metabolism; D017353:Gene Deletion; D006384:Hemagglutination / Q000502:physiology; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D006388:Hemagglutinins; C118973:hemagglutinin 33 protein, Clostridium botulinum; D001905:Botulinum Toxins