Antioxidative activity of natural isorhapontigenin.
Isorhapontigenin (ISOR), isolated from Belamcanda chinensis, is a derivative of stilbene. Its chemical structure is very similar to that of resveratrol, with a potent antioxidative effect. In the present study, we investigated the antioxidative activity of ISOR in vitro. Oxidative damage of rat liver microsomes, brain mitochondria and synaptosomes was induced by Fe2+-Cys, VitC-ADP-Fe2+ and H2O2, respectively. The formation of malondialdehyde (MDA), decrease of reduced glutathione (GSH) and increase of ultra-weak chemiluminescence during the lipid peroxidation process were determined. In addition, the characteristic ultra-weak chemiluminescence of oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2 system was studied. The results showed that ISOR significantly inhibited MDA formation in liver microsomes, brain mitochondria and synaptosomes induced by Fe2+-Cys. Also, ISOR markedly prevented the decrease of GSH in mitochondria and synaptosomes induced by H2O2 and the increase of ultra-weak chemiluminescence during lipid peroxidation induced by VitC-ADP-Fe2+ as well as oxidative DNA damage induced by CuSO4-Phen-VitC-H2O2. The effects of ISOR at 10(-5) and 10(-6) mol/L on the MDA formation and decrease of GSH were similar to that of the classical antioxidant vitamin E (10(-4) mol/L). It may be concluded that ISOR possessed potent antioxidative activity and was much more potent than vitamin E.
Wang
Q L
QL
2nd Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
Lin
M
M
Liu
G T
GT
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects*; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D015394:Molecular Structure; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013574:Synaptosomes / Q000187:drug effects
D000975:Antioxidants; D016861:Cyclooxygenase Inhibitors; D013267:Stilbenes; C432307:isorhapontigenin; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D005978:Glutathione