title: Use of molecular probes to assess geographic distribution of Pfiesteria species.
abstract: We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems.
authors.affiliation: Biology Department, University of North Carolina at Greensboro, 27402, USA. rublee@uncg.edu
authors.forename: P A
authors.initials: PA
authors.lastname: Rublee
authors.forename: J W
authors.initials: JW
authors.lastname: Kempton
authors.forename: E F
authors.initials: EF
authors.lastname: Schaefer
authors.forename: C
authors.initials: C
authors.lastname: Allen
authors.forename: J
authors.initials: J
authors.lastname: Harris
authors.forename: D W
authors.initials: DW
authors.lastname: Oldach
authors.forename: H
authors.initials: H
authors.lastname: Bowers
authors.forename: T
authors.initials: T
authors.lastname: Tengs
authors.forename: J M
authors.initials: JM
authors.lastname: Burkholder
authors.forename: H B
authors.initials: HB
authors.lastname: Glasgow
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005393:Fish Diseases; D005843:Geography; D006801:Humans; D020045:Pfiesteria piscicida / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011528:Protozoan Infections; D011634:Public Health
chemical_list: D016054:DNA, Protozoan