Comprehensive analysis of a large genomic sequence at the putative B-cell chronic lymphocytic leukaemia (B-CLL) tumour suppresser gene locus.
In many haematological diseases, and more particularly in B-cell chronic lymphocytic leukaemia (B-CLL), the existence of a tumour suppressor gene located within the frequently deleted region 13q14.3, has been put forward. A wide candidate region spanning from marker D13S273 to D13S25 has been proposed and an extensive physical map has been constructed by several teams. In this study, we sequenced a minimal core deleted region that we have previously defined and annotated it with flanking available public sequences. Our analysis shows that this region is gene-poor. Furthermore, our work allowed us to identify new alternative transcripts, spanning core regions, of the previously defined candidate genes DLEU1 and DLEU2. Since their putative involvement in B-CLL was controversial, our present study provide support for reconsidering the DLEU1 and DLEU2 genes as B-CLL candidate genes, with a new definition of their organisation and context.
Rondeau
G
G
INSERM U463, Institut de Biologie de l'Hôtel-Dieu, Centre Hospitalier Universitaire, 44093 Cedex, Nantes, France.
Moreau
I
I
Bézieau
S
S
Petit
J L
JL
Heilig
R
R
Fernandez
S
S
Pennarun
E
E
Myers
J S
JS
Batzer
M A
MA
Moisan
J P
JP
Devilder
M C
MC
D017398:Alternative Splicing / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism*; D001483:Base Sequence; D002874:Chromosome Mapping; D030561:Databases, Nucleic Acid; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D016147:Genes, Tumor Suppressor; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion / Q000235:genetics*