title: CD4+ Th1-cells predominate in low-grade B-cell lymphoma of gastric mucosa-associated lymphoid tissue (MALT type).
abstract: BACKGROUND
  Little is known about the function of T cells in the inflammatory infiltrate in Helicobacter pylori-associated gastritis and B-cell lymphoma of mucosa-associated lymphoid tissue (MALT type). Previous studies have proposed a dominant Th1-type response in low-grade MALT lymphoma consistent with the Th1 response observed in H. pylori-associated gastritis.
  
  
  METHODS
  We performed a novel flow cytometric approach in which CD3 panning for enrichment and activation of small numbers of T cells and intracellular cytokine analysis were combined to selectively characterize the cytokine profile of T cells (IFN-gamma for Th1) derived from the gastric mucosa of 23 patients with low-grade MALT lymphoma stage IEI1 (lymphoma infiltration of mucosa/submucosa sparing the muscularis). Endosonography was performed in each case to control the depth of lymphoma infiltration. For comparison, 19 patients with H. pylori-positive gastritis were also analysed.
  
  
  RESULTS
  There was a CD4/CD8 ratio of 4 in patients with MALT lymphoma and of 2 in chronic gastritis. The proportion of IFN-gamma producing cells within the CD4-positive T-cell population in MALT lymphoma was 22%; in chronic gastritis it was 13% while no such difference could be encountered in CD8-positive T cells.
  
  
  CONCLUSIONS
  The data point towards a dominant intratumoral IFN-gamma dominated T-cell response associated with early low-grade MALT lymphoma. A polarized IFN-gamma dominated Th1-type response may either contribute to the inability of the immune system to eradicate H. pylori infection, thereby promoting the activation status of the lymphocytic infiltrate in low-grade MALT lymphoma, or may mirror a concomitant tumor-specific T-cell response accompanying early stages of tumor progression.
authors.affiliation: Dept. of Medicine B, Institute for Immunology and Institute for Microbiology, University of Münster, Germany. sriedel@uni-muenster.de
authors.forename: S
authors.initials: S
authors.lastname: Riedel
authors.forename: M
authors.initials: M
authors.lastname: Kraft
authors.forename: T
authors.initials: T
authors.lastname: Kucharzik
authors.forename: H G
authors.initials: HG
authors.lastname: Pauels
authors.forename: M
authors.initials: M
authors.lastname: Tiemann
authors.forename: A
authors.initials: A
authors.lastname: Steinbüchel
authors.forename: W
authors.initials: W
authors.lastname: Domschke
authors.forename: N
authors.initials: N
authors.lastname: Lügering
mesh_terms: D000328:Adult; D000368:Aged; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018442:Lymphoma, B-Cell, Marginal Zone / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000276:immunology*
chemical_list: D007371:Interferon-gamma