Molecular identification and characterization of turkey IFN-gamma gene.
The cDNAs of turkey and chicken interferon gamma (IFN-gamma) were cloned and the functional activity of turkey and chicken IFN-gamma was compared. The coding region of turkey IFN-gamma gene encodes a predicted mature protein of 145 amino acids with a molecular weight at 16.8 kDa. Compared with type I IFN, the IFN-gamma between turkey and chicken also had the same size and high degree of identity at the nucleotide (96.0%) and amino acid (96.4%) sequence. Turkey IFN-gamma was cross-reactive with chicken cells. Both turkey and chicken IFN-gamma could induce production of nitric oxide by turkey or chicken macrophages. Turkey IFN-gamma also had similar degree of sensitivity to heat and pH 2.0 as chicken IFN-gamma. The functional activity of both turkey and chicken IFN-gamma could be neutralized by a monoclonal antibody specific to chicken IFN-gamma to a similar extent. These results indicated that IFN-gamma protein was cross-reactive between turkey and chicken.
Loa
C C
CC
Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907-1175, USA.
Hsieh
M K
MK
Wu
C C
CC
Lin
T L
TL
D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007371:Interferon-gamma / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013997:Time Factors; D014162:Transfection; D014422:Turkeys
D018076:DNA, Complementary; D011994:Recombinant Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma