title: Individual variations in the correlation between erythemal threshold, UV-induced DNA damage and sun-burn cell formation.
abstract: A linear correlation between erythema intensity and DNA damage upon exposure to UV has not been firmly established. Many of the deleterious effects of UV exposure do occur after exposure to suberythemal doses. After DNA damage, cells undergo DNA repair. It is commonly accepted that when the burden of damage is beyond the repair capacities, the cell undergoes programmed cell death or apoptosis. The aim of this study is to quantify the amount of UV-induced DNA damage (estimated via the measurement of DNA repair or unscheduled DNA synthesis or UDS) and cellular damage (estimated via the determination of the density of sunburn cells or SBC). If DNA damage and erythema are correlated, similar intensity of UDS and similar density of SBC should be found in volunteers irradiated with a UV dose equal to two minimal erythema doses (MED). Our results show that in 15 different individuals the same relative dose (2 MEDs) provokes UDS values, which vary within a factor of 4. An even larger variability affects SBC counts after the same relative dose. When DNA damage or SBC are plotted versus the absolute dose (i.e. the dose expressed in J/m(2)), there is a rough correlation (with several exceptions) between dose and extent of UDS and SBC counts. It seems possible to divide the volunteers into two subpopulations with different susceptibilities to UV damage. It is well known that UDS and SBC measurements are often affected by large experimental indeterminacy, yet, the analysis of our results makes it plausible to suggest that for the triggering of erythema, a common threshold value for DNA damage or for SBC count are not to be found. In conclusion, the erythema response seems to be loosely correlated with DNA damage. This suggests that the protection offered by the sunscreens against DNA damage, the molecular basis of UV-induced mutagenesis, might not be related to the sun protection factor (SPF) indicated on the label of sunscreens, which is evaluated using the erythema as an endpoint.
authors.affiliation: Department of Dermatology, Hôpital Académique Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. mheenen@ulb.ac.be
authors.forename: M
authors.initials: M
authors.lastname: Heenen
authors.forename: P U
authors.initials: PU
authors.lastname: Giacomoni
authors.forename: P
authors.initials: P
authors.lastname: Golstein
mesh_terms: D000016:Abnormalities, Radiation-Induced / Q000235:genetics* / Q000473:pathology; D000328:Adult; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004890:Erythema / Q000235:genetics* / Q000473:pathology; D006801:Humans; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D016082:Solar System; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D004247:DNA