Distribution of cytoskeletal proteins, integrins, leukocyte adhesion molecules and extracellular matrix proteins in plastic-embedded human and rat kidneys.
OBJECTIVE
To study the distribution of cytoskeletal proteins (actin, alpha-actinin, vinculin, beta-tubulin, keratin, vimentin, desmin), adhesion molecules for cell-matrix interations (very later antigens [VLA1-61, beta1, beta2 [CD18], vitronectin receptor [alphavbeta3], CD 11b), leukocyte adhesion molecules (ICAM-1) and extracellular matrix proteins (collagen IV, fibronectin, laminin, vitronectin) in human and rat kidneys by using a superior processing and immunohistochemical staining technique.


METHODS
Human and rat kidneys were fixed in 2% paraformaldehyde, dehydrated in acetone and processed in a new, low-toxic glycol, methacrylate mixture, especially developed for immunohistochemistry. Both the glomeruli and the interstitial areas were carefully examined and scored semiquantitatively.


RESULTS
Immunostained plastic sections showed excellent morphology combined with remarkably well preserved antigenicity.


CONCLUSIONS
The above mentioned provides an excellent tool for the accurate localization of a wide variety of antigens at the light microscopic level.
van Goor
H
H
Department of Pathology and Laboratory Medicine, University Hospital Groningen, The Netherlands.
Coers
W
W
van der Horst
M L
ML
Huitema
S
S
Suurmeijer
A J
AJ
D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000032:analysis*; D004727:Endothelium / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D039222:Integrin alpha1beta1; D016023:Integrins / Q000032:analysis*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D008297:Male; D016613:Plastic Embedding / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains
D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D039222:Integrin alpha1beta1; D016023:Integrins; D018799:Intercellular Adhesion Molecule-1