An extensive class of small RNAs in Caenorhabditis elegans.
The lin-4 and let-7 antisense RNAs are temporal regulators that control the timing of developmental events in Caenorhabditis elegans by inhibiting translation of target mRNAs. let-7 RNA is conserved among bilaterian animals, suggesting that this class of small RNAs [microRNAs (miRNAs)] is evolutionarily ancient. Using bioinformatics and cDNA cloning, we found 15 new miRNA genes in C. elegans. Several of these genes express small transcripts that vary in abundance during C. elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse.
Lee
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RC
Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA.
Ambros
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D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence; D021901:DNA, Intergenic; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017238:Genes, Helminth; D006801:Humans; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D016372:RNA, Antisense / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018510:RNA, Helminth / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D021901:DNA, Intergenic; D012322:RNA Precursors; D016372:RNA, Antisense; D018510:RNA, Helminth; D022661:RNA, Untranslated