Mouse testis-brain RNA-binding protein (TB-RBP): expression, purification and crystal X-ray diffraction.
TB-RBP (testis-brain RNA-binding protein) is a mouse RNA-binding protein that controls the temporal and spatial expression of mRNA. Found most abundantly in brain and male germ cells in the testis, TB-RBP is known to suppress the translation of specific testicular and brain mRNAs as part of cell development. TB-RBP-mRNA complexes can bind microtubules and thereby facilitate RNA translocation. Translin is the human orthologue of TB-RBP which binds to single-stranded ends of DNA sequences in breakpoint regions of chromosomal translocations. TB-RBP/translin has been crystallized in space group P2(1)2(1)2. The expression, purification, and crystallization of TB-RBP are described as well as preliminary X-ray diffraction data. The multimeric state of TB-RBP is addressed using dynamic light-scattering results.
Pascal
J M
JM
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA. jrobertus@mail.utexas.edu
Chennathukuzhi
V M
VM
Hecht
N B
NB
Robertus
J D
JD
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000737:chemistry*; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry*
D008869:Microtubule-Associated Proteins; D016601:RNA-Binding Proteins; C490551:STRBP protein, human