title: Analysis of reboxetine, a novel antidepressant drug, in pharmaceutical tablets by capillary electrophoresis and derivative spectrophotometry.
abstract: The recent antidepressant drug reboxetine was quantified in pharmaceutical tablets by derivative spectrophotometry and capillary zone electrophoresis. The feasible sample pretreatment consists of a single extraction with a pH 2.5 phosphate buffer, centrifugation and dilution. For the spectrophotometric assay, the fourth derivative of the absorbance was used which gave satisfactory results in terms of accuracy (mean recovery 99.7%) and precision (mean RSD 3.4%). The electrophoretic experiments were carried out using the shortest effective length of the capillary (8.5 cm) in order to obtain a very rapid separation of reboxetine and dibenzepine used as the internal standard. Using a pH 2.5, 50 mM phosphate buffer as the background electrolyte, each analysis lasted less than 2.5 min. Accuracy (101.3%) and precision (1.5%) were very good.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy. raggima@alma.unibo.it
authors.forename: M A
authors.initials: MA
authors.lastname: Raggi
authors.forename: F
authors.initials: F
authors.lastname: Bugamelli
authors.forename: C
authors.initials: C
authors.lastname: Sabbioni
authors.forename: A
authors.initials: A
authors.lastname: Ferranti
authors.forename: S
authors.initials: S
authors.lastname: Fanali
authors.forename: V
authors.initials: V
authors.lastname: Volterra
mesh_terms: D000928:Antidepressive Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D015394:Molecular Structure; D009025:Morpholines / Q000032:analysis* / Q000737:chemistry; D000077593:Reboxetine; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets / Q000032:analysis
chemical_list: D000928:Antidepressive Agents; D009025:Morpholines; D013607:Tablets; D000077593:Reboxetine