Interleukin-1beta isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines.
The gilthead seabream IL-1beta gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5' untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3'UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1beta sequences. The IL-1beta message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1beta was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation.
Pelegrín
P
P
Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
García-Castillo
J
J
Mulero
V
V
Meseguer
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021541:Sea Bream / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D016415:Sequence Alignment; D015854:Up-Regulation
D007375:Interleukin-1; D008070:Lipopolysaccharides; D008222:Lymphokines