Prognostic significance of immuno-proteosome subunit expression in patients with renal-cell carcinoma: a preliminary study.
Our purpose was to elucidate the clinical roles of the "immuno-proteosome," which is involved in the accelerated pathway of the major histocompatibility complex (MHC) class I-restricted antigen presentation system, in renal cell carcinoma (RCC). The relative expression of six proteosome subunits (existing subunits X, Y, and Z and immunoproteosome subunits LMP7, LMP2, and MECL1) in 54 RCCs was investigated using RT-PCR analysis and was compared with clinicopathological measures, including patient outcome. Expression of the LMP7 and LMP2 genes was significantly low in high-grade tumors, and that of the LMP7 and MECL1 genes was significantly low in high-stage tumors. Low levels of LMP7, LMP2, and MECL1 expression were strongly associated with shortened survival (LMP7: P = 0.0002, LMP2: P < 0.0001, MECL1: P < 0.0047). The levels of subunits X, Y, and Z had no significant correlation with those measures. These findings suggest that RCCs with low level of immuno-proteosome subunit expression have a disorder in their antigen-presentation system. As a consequence, they may escape from immune surveillance and worsen patient outcome.
Murakami
Y
Y
Department of Urology, School of Medicine, The University of Tokushima, Tokushima, Japan. ymura@nb.tcn.ne.jp
Kanda
K
K
Yokota
K
K
Kanayama
H
H
Kagawa
S
S
D017951:Antigen Presentation; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000276:immunology / Q000503:physiopathology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000276:immunology / Q000503:physiopathology; D008297:Male; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D013223:Statistics as Topic; D015996:Survival Rate; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D015395:Histocompatibility Antigens Class I; D009097:Multienzyme Complexes; D021122:Protein Subunits; D011506:Proteins; D014763:Viral Matrix Proteins; D003546:Cysteine Endopeptidases; C077436:LMP7 protein; C098948:PSMB10 protein, human; D046988:Proteasome Endopeptidase Complex