Progress toward analysis of D8/17 binding to B cells in children with obsessive compulsive disorder and/or chronic tic disorder.
BACKGROUND
Previous research has suggested that a subgroup of children with obsessive compulsive disorder (OCD) have neuropsychiatric sequelae of streptococcal pharyngitis, similar to that seen in the neurological manifestation of rheumatic fever (RF). Monoclonal antibody D8/17 demonstrates increased binding to B cells in patients with RF and in patients with neuropsychiatric disorders using immunofluorescent microscopy.


OBJECTIVE
The aim of this study was to determine if an earlier immunofluorescent microscopy study of monoclonal antibody D8/17 in childhood-onset OCD and/or chronic tic disorder (CTD) could be replicated using the more objective method of flow cytometric analysis.


METHODS
D8/17 binding to B cells was determined in patients with OCD and or CTD (N=32), and healthy controls (N=12) by flow cytometric analysis.


RESULTS
Subjects with OCD/CTD showed increased mean cell binding (26.0%) of monoclonal antibody compared with healthy controls (9.1%) (p<0.001). When using the threshold of greater than 19% binding (95% upper confidence interval) as a measure of positivity, 65.6% of patients compared with 8.3% of controls showed increased antibody binding to B cells (p=0.01).


CONCLUSIONS
Although this study reports positive results, many methodological issues will need to be addressed before generalized use of assay for diagnostic purposes.
Murphy
T K
TK
Syndrome Clinic, Department of Psychiatry, University of Florida, P.O. Box 100256, Gainesville, FL 32610, USA. tmurphy@psych.med.ufl.edu
Benson
N
N
Zaytoun
A
A
Yang
M
M
Braylan
R
R
Ayoub
E
E
Goodman
W K
WK
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000276:immunology; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D009771:Obsessive-Compulsive Disorder / Q000175:diagnosis / Q000276:immunology* / Q000503:physiopathology; D012737:Sex Factors; D013290:Streptococcal Infections / Q000150:complications* / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*; D013981:Tic Disorders / Q000175:diagnosis / Q000276:immunology* / Q000503:physiopathology
D000911:Antibodies, Monoclonal