Over-expression of FoxM1 stimulates cyclin B1 expression.
FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role of FoxM1 and its downstream regulatory targets, three stably transfected HeLa lines that display doxycycline (dox)-inducible FoxM1 expression were established. Over-expression of FoxM1 by dox induction facilitates growth recovery from serum starvation. Quantitation of cyclin B1 and D1 levels using flow cytometric, Western and Northern analyses reveals that elevated FoxM1 levels lead to stimulation of cyclin B1 but not cyclin D1 expression. Transient reporter assays in the dox-inducible lines and upon co-transfection with a constitutive FoxM1 expression plasmid suggest that FoxM1 can activate the cyclin B1 promoter.
Leung
T W
TW
Department of Biochemistry, Faculty of Medicine, University of Hong Kong, China.
Lin
S S
SS
Tsang
A C
AC
Tong
C S
CS
Ching
J C
JC
Leung
W Y
WY
Gimlich
R
R
Wong
G G
GG
Yao
K M
KM
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division; D016895:Culture Media, Serum-Free; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D056744:Cyclin B1; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004318:Doxycycline / Q000494:pharmacology; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
C534699:CCNB1 protein, human; C534700:Ccnb1 protein, mouse; D016895:Culture Media, Serum-Free; D019926:Cyclin B; D056744:Cyclin B1; C107585:FOXM1 protein, human; D000072278:Forkhead Box Protein M1; D051858:Forkhead Transcription Factors; C491524:Foxm1 protein, mouse; D014157:Transcription Factors; D019938:Cyclin D1; D004247:DNA; D008156:Luciferases; D004318:Doxycycline