Clinical evaluation of human telomerase catalytic subunit in bladder washings from patients with bladder cancer.
OBJECTIVE
We examined the expression of mRNA of human telomerase reverse transcriptase (hTERT), a catalytic subunit of the telomerase complex, in bladder washings as a tumor marker for the detection of bladder cancer.


METHODS
Bladder washings were obtained from 63 patients. We examined the expression of hTERT mRNA by the nested reverse transcription-polymerase chain reaction (RT-PCR) method and also measured the relative expressions of hTERT mRNA and beta(2)-microglobulin (beta(2)-MG) in these samples by RT-PCR analysis. Correlations between the relative expression of hTERT mRNA and clinical features were analyzed. We also compared the sensitivity of this assay with that of urinary cytology.


RESULTS
By nested RT-PCR, we detected three false-positive cases (11%) in the control group. Therefore, the relative expression values of hTERT mRNA and beta(2)-MG correlated strongly with tumor size, but not with multiplicity or histologic grade. When the cut-off value of the expression was fixed at 0.27%, the sensitivity and specificity of this assay were 74% and 93%, respectively. This assay was more sensitive than urinary cytology for the detection of bladder cancer.


CONCLUSIONS
These results suggest that the relative expression of hTERT mRNA in bladder washings is useful in screening for bladder cancer. Relative expression is of assistance in diagnosing bladder cancer.
Fukui
T
T
Department of Specific Organ Regulation, Urology, Osaka University Graduate School of Medicine, Osaka, Japan.
Nonomura
N
N
Tokizane
T
T
Sato
E
E
Ono
Y
Y
Harada
Y
Y
Nishimura
K
K
Takahara
S
S
Okuyama
A
A
D014408:Biomarkers, Tumor / Q000032:analysis; D004268:DNA-Binding Proteins; D003951:Diagnostic Errors; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000032:analysis* / Q000737:chemistry; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000201:enzymology; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000652:urine; D014556:Urine / Q000166:cytology; D001620:beta-Thromboglobulin / Q000032:analysis
D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D001620:beta-Thromboglobulin; D019098:Telomerase