Characterization of the immunophenotype and the metastatic properties of a murine T-lymphoma cell line. Unexpected expression of cytoplasmatic CD4.
We report the first characterization of a mouse T-lymphoma cell line that surprisingly expresses cytoplasmatic (cy) yCD4. Phenotypically, LBC cells are CD5+, CD8+, CD16+, CD24+, CD25+, CD2-/dim, CD3-/dim, TCRbeta-/dim, TCRgammadelta, CD154 , CD40-, and CD45R. Coexpress cyTCRbeta, cyCD3, cyCD4, and yet lack surface CD4 expression. Transplantation of LBC cells into mice resulted in an aggressive T-lymphoblastic lymphoma that infiltrated lymph nodes, thymus, spleen, liver, ovary, and uterus but not peripheral blood or bone marrow. LBC cells display a modal chromosome number of 39 and a near-diploid karyotype. Based on the characterization data, we demonstrated that the LBC cell line was derived from an early T-cell lymphocyte precursor. We propose that the malignant cell transformation of LBC cells could coincide with the transition stage from late double-negative, DN3 (CD4- CD8 CD44-/low, CD25+) or DN4 (CD4-low, CD8-/low, CD44-, CD25-) to double-positive (DP: CD4+CD8+) stage of T-cell development. LBC cells provide a T-lymphoblastic lymphoma model derived from a malignant early T-lymphocyte that can be potentially useful as a model to study both cellular regulation and differentiation of T-cells. In addition, LBC tumor provides a short latency neoplasm to study cellular regulation and to perform preclinical trials of lymphoma-relatel clisorders.
Mongini
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C
Institut de Estudios de la Inmunidad Humoral, UBA-CONICET, Department of Immunology, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina. cmp@huemul.ffyb.uba.ar
Ruybal
P
P
Gravisaco
M J
MJ
Croci
M
M
Sánchez Lockhart
M
M
Fabris
V
V
Waldner
A C
AC
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis*; D005434:Flow Cytometry; D016130:Immunophenotyping; D007621:Karyotyping; D008099:Liver / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D013154:Spleen / Q000473:pathology; D013950:Thymus Gland / Q000473:pathology; D014407:Tumor Cells, Cultured
D015704:CD4 Antigens