title: Use of protein-protein interactions in affinity chromatography.
abstract: Biospecific recognition between proteins is a phenomenon that can be exploited for designing affinity-chromatographic purification systems for proteins. In principle, the approach is straightforward, and there are usually many alternative ways, since a protein can be always found which binds specifically enough to the desired protein. Routine immunoaffinity chromatography utilizes the recognition of antigenic epitopes by antibodies. However, forces involved in protein-protein interactions as well the forces keeping the three-dimensional structures of proteins intact are complicated, and proteins are easily unfolded by various factors with unpredictable results. Because of this and because of the generally high association strength between proteins, the correct adjustment of binding forces between an immobilized protein and the protein to be purified as well as the release of bound proteins in biologically active form from affinity complexes are the main problem. Affinity systems involving interactions like enzyme-enzyme, subunit-oligomer, protein-antibody, protein-chaperone and the specific features involved in each case are presented as examples. This article also aims to sketch prospects for further development of the use of protein-protein interactions for the purification of proteins.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation. vimuronets@mail.ru
authors.forename: V I
authors.initials: VI
authors.lastname: Muronetz
authors.forename: M
authors.initials: M
authors.lastname: Sholukh
authors.forename: T
authors.initials: T
authors.lastname: Korpela
mesh_terms: D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D004798:Enzymes / Q000737:chemistry / Q000302:isolation & purification; D011489:Protein Denaturation; D017510:Protein Folding; D021122:Protein Subunits; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D004798:Enzymes; D021122:Protein Subunits; D011506:Proteins