Quantitative determination of medroxyprogesterone acetate in plasma by liquid chromatography/electrospray ion trap mass spectrometry.
A sensitive and rapid liquid chromatography/electrospray ion trap mass spectrometry (LC/MS/MS) method has been developed for the quantitative determination of medroxyprogesterone acetate (MPA) in human plasma. Plasma samples (1.0 mL) were simply extracted with pentane and the extracts were analyzed by HPLC with the detection of the analyte in the selective reaction monitoring (SRM) mode. The determination of MPA was accurate and reproducible, with a limit of quantitation of 0.05 ng/mL in plasma. The standard calibration curve for MPA was linear (r = 0.998) over the concentration range 0.05-6.0 ng/mL in human plasma. Analysis precision over the concentration range of MPA was lower than 18.8% (relative standard deviation, RSD) and accuracy was between 96.2 and 108.7%.
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Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, PO Box 131, Chungryang, Seoul, Korea.
Kim
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D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003279:Contraceptives, Oral, Sequential / Q000097:blood*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008525:Medroxyprogesterone / Q000097:blood*; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization
D003279:Contraceptives, Oral, Sequential; D007202:Indicators and Reagents; D012996:Solutions; D008525:Medroxyprogesterone