Determination of amino acid tissue concentrations by microdialysis: method evaluation and relation to plasma values.
Microdialysis is an in vivo technique to monitor tissue concentrations of low molecular weight substances by means of a continuously perfused artificial capillary with a semipermeable membrane placed into the region of interest. The suitability of microdialysis to determine tissue concentrations of amino acids was evaluated in vitro by placing the catheter into Ringer buffer or into a plasma protein (50g/l) solution containing 32 different amino acids (150 micromol/l each). All amino acids tested crossed freely the microdialysis membrane with recoveries close to 100%. Microdialysis fluid was sampled from subcutaneous tissue of five newborns and amino acid content analysed. Total and non protein bound amino acids were determined in the patients plasma by acid precipitation or ultrafiltration, respectively. Mean subcutaneous tissue concentrations were lower as compared to plasma for taurine, serine, alanine, aspartate, glutamate and ornithine and higher for valine, isoleucine, leucine, methionine, phenylalanine, tyrosine and arginine, indicating net uptake or release of amino acids from subcutaneous tissue. Thus, microdialysis offers a convenient and minimal invasive way to study tissue amino acid composition and appears to be a promising analytical tool for the study of amino acid metabolism in vivo.
Rolinski
B
B
Department of Clinical Chemistry and Metabolic Disorders Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, Munich, Federal Republic of Germany. Rolinski@kk-i.med.uni-muenchen.de
Baumeister
F A
FA
Roscher
A A
AA
D000596:Amino Acids / Q000032:analysis* / Q000097:blood*; D002408:Catheters, Indwelling; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D017551:Microdialysis / Q000379:methods*
D000596:Amino Acids