title: Oximidines I and II: Novel Antitumor Macrolides from Pseudomonas sp.
abstract: Our screening for cell-cycle inhibitors in transformed cells resulted in the isolation of novel active compounds, oximidines I (1) and II (2), from Pseudomonas sp. Q52002. The molecular formulas of 1 and 2 were established as C(23)H(24)N(2)O(7) and C(23)H(24)N(2)O(6), respectively, by high-resolution FABMS. The structures of the oximidines were elucidated by NMR spectral analysis, including a variety of two-dimensional techniques. The absolute stereochemistry of 1 was determined by using the modified Mosher method. The oximidines are novel 12-membered macrolides containing an O-methyloxime moiety. Oximidines I and II selectively inhibited the growth of rat 3Y1 cells transformed with E1A, ras, or src oncogenes.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan, and Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shin-Ya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Furihata
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Seto