title: Plexin-A3 mediates semaphorin signaling and regulates the development of hippocampal axonal projections.
abstract: Plexins are receptors implicated in mediating signaling by semaphorins, a family of axonal chemorepellents. The role of specific plexins in mediating semaphorin function in vivo has not, however, yet been examined in vertebrates. Here, we show that plexin-A3 is the most ubiquitously expressed plexin family member within regions of the developing mammalian nervous system known to contain semaphorin-responsive neurons. Using a chimeric receptor construct, we provide evidence that plexin-A3 can transduce a repulsive signal in growth cones in vitro. Analysis of plexin-A3 knockout mice shows that plexin-A3 contributes to Sema3F and Sema3A signaling and that plexin-A3 regulates the development of hippocampal axonal projections in vivo.
authors.affiliation: Department of Anatomy, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94143-0452, USA.
authors.forename: H J
authors.initials: HJ
authors.lastname: Cheng
authors.forename: A
authors.initials: A
authors.lastname: Bagri
authors.forename: A
authors.initials: A
authors.lastname: Yaron
authors.forename: E
authors.initials: E
authors.lastname: Stein
authors.forename: S J
authors.initials: SJ
authors.lastname: Pleasure
authors.forename: M
authors.initials: M
authors.lastname: Tessier-Lavigne
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D015153:Blotting, Western; D005260:Female; D005727:Ganglia, Spinal / Q000737:chemistry / Q000196:embryology; D015870:Gene Expression; D006023:Glycoproteins / Q000378:metabolism*; D006624:Hippocampus / Q000254:growth & development* / Q000648:ultrastructure*; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016254:Mutagenesis, Insertional; D009419:Nerve Tissue Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040121:Semaphorin-3A; D015398:Signal Transduction; D013116:Spinal Cord / Q000737:chemistry / Q000196:embryology; D017783:Superior Cervical Ganglion / Q000737:chemistry / Q000196:embryology; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000196:embryology; D014981:Xenopus
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C492112:Plxna3 protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C491229:Sema3f protein, mouse; D040121:Semaphorin-3A