Neurocalcin-actin interaction.
Neurocalcin is an N-myristoylated calcium-binding protein which belongs to a novel family of neuronal calcium sensors. Here we show, by cosedimentation, co-immunoprecipitation and cross-linking approaches, that myristoylated neurocalcin directly interacts with actin in a calcium-dependent manner. We used EDC cross-linking and obtained one novel 64 kDa entity composed of one actin molecule and one neurocalcin molecule, as demonstrated with IAEDANS-actin and neurocalcin-specific antibodies. This interaction could modulate the rod outer segment-guanylate cyclase 1-neurocalcin interface.
Mornet
D
D
Muscles et Pathologies, INSERM U128, IFR 24, Institut Bouisson-Bertrand, 778, Rue de la Croix Verte, 34196 Cedex 5, Montpellier, France.
Bonet-Kerrache
A
A
D000199:Actins / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000737:chemistry; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D002417:Cattle; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D051596:Neurocalcin; D011233:Precipitin Tests; D011817:Rabbits; D044169:Receptors, Calcium-Sensing
D000199:Actins; D002135:Calcium-Binding Proteins; D003432:Cross-Linking Reagents; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing; D002118:Calcium