Modulation of the expression of matrix metalloproteinase and tissue inhibitors of metalloproteinases by TGF-beta1 and IGF-1 in primary human articular and bovine nasal chondrocytes stimulated with TNF-alpha.
Tumour necrosis factor-alpha (TNF-alpha) was able to promote collagen breakdown from bovine cartilage in explant culture. This release was dependent upon matrix metalloproteinases (MMPs) and could be prevented by transforming growth factor-beta1 (TGF-beta1) or insulin-like growth factor-1. Both growth factors reduced the expression and secretion of collagenase enzymes, and TGF-beta1 induced tissue inhibitor of metalloproteinase production. This study shows for the first time that these anabolic growth factors can protect cartilage against TNF-alpha-induced destruction.
Hui
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Department of Rheumatology, Medical School, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK.
Rowan
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AD
Cawston
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T
D000818:Animals; D015152:Blotting, Northern; D002358:Cartilage, Articular / Q000187:drug effects* / Q000201:enzymology; D002417:Cattle; D019902:Chondrocytes / Q000187:drug effects* / Q000201:enzymology; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
C508960:TGFB1 protein, human; D019714:Tissue Inhibitor of Metalloproteinases; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D020782:Matrix Metalloproteinases