title: VO2 slow component correlates with vastus lateralis de-oxygenation and blood lactate accumulation during running.
abstract: BACKGROUND
  In the present study, vastus lateralis de-oxygenation was monitored contemporarily with VO2 changes along a severe constant intensity running exercise, after the 3rd min up to volitional exhaustion. Blood lactate accumulation was also measured before, during and after running.
  
  
  METHODS
  Eleven male amateur soccer players volunteered for the study. Subjects mean age, height, and body weight were 22.9+/-2 yrs, 177.5+/-6.2 cm, 71.7+/-4 kg, respectively. Measurements were carried out during running on a treadmill. Ventilatory and gas exchange parameters were measured at the mouth on a breath-by-breath basis. For blood lactate concentration accumulation measurement, capillary blood samples were taken from the fingertip. The oxygenation of the vastus lateralis muscle were measured by a continuous wave NIRS portable instrument. By means of two pretests the onset of [La]b accumulation and its associated velocity (vOBLA), and the peak of oxygen uptake and its associated velocity (vVO2,peak) were assessed. The test consisted of running on the treadmill up to volitional exhaustion at a constant velocity corresponding to vOBLA plus 50% of the difference between vVO2,peak and vOBLA (v50%Delta).
  
  
  RESULTS
  The principal finding of this study was that vastus lateralis de-oxygenation changes measured during running correlate with a) oxygen uptake changes between the 3rd min of exercise and the time corresponding to the subject's volitional exhaustion; b) blood lactate concentration increments measured at the 3rd and the 6th min of exercise and at the time corresponding to the subject's volitional exhaustion.
  
  
  CONCLUSIONS
  In conclusion, the results of the present study support our hypothesis that the vastus lateralis de-oxygenation contributes consistently to the VO2 slow component development in running.
authors.affiliation: University Institute of Motor Sciences, Rome, Italy. s.demarie@libero.it
authors.forename: S
authors.initials: S
authors.lastname: Demarie
authors.forename: V
authors.initials: V
authors.lastname: Quaresima
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
authors.forename: F
authors.initials: F
authors.lastname: Sardella
authors.forename: V
authors.initials: V
authors.lastname: Billat
authors.forename: M
authors.initials: M
authors.lastname: Faina
mesh_terms: D000328:Adult; D015308:Anaerobic Threshold; D000704:Analysis of Variance; D006801:Humans; D019344:Lactic Acid / Q000097:blood*; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D012420:Running / Q000502:physiology*
chemical_list: D019344:Lactic Acid