Tomato yellow leaf curl China virus: monopartite genome organization and agroinfection of plants.
The complete DNA sequence (2734 nucleotides) of the monopartite genome of tomato yellow leaf curl China virus (TYLCCNV), a begomovirus transmitted by the whitefly Bemisia tabaci, was determined. The circular genomic DNA contains six open reading frames (ORFs) encoding proteins of molecular weights >10 kDa, of which two (V1 and V2) are located on the virion-sense strand and four (C1, C2, C3 and C4) on the complementary-sense strand. The ORFs are comparable to those of other whitefly-transmitted begomoviruses with a monopartite genome and to those encoded by DNA-A of bipartite begomoviruses. Sequence comparisons with other geminiviruses showed that TYLCCNV belongs to Begomovirus from the Old World. No putative DNA-B genome was found. Nicotiana species and tomato plants agroinoculated with the TYLCCNV monopartite genome developed typical yellowing and leaf-curling symptoms. The cloned molecule carried all the information needed for virus replication and systemic infection of plants.
Yin
Q
Q
Department of Molecular Virology and Bioengineering, Institute of Microbiology, CAS, 100080, Beijing, PR China.
Yang
H
H
Gong
Q
Q
Wang
H
H
Liu
Y
Y
Hong
Y
Y
Tien
P
P
D002681:China; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D017791:Geminiviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology*
D004279:DNA, Viral