title: Zebrafish mab21l2 is specifically expressed in the presumptive eye and tectum from early somitogenesis onwards.
abstract: Random screening for tissue specific genes in zebrafish by in situ hybridization led us to isolate a gene which showed highly restricted expression in the developing eyes and midbrain at somitogenesis stages. This gene was very similar to mouse and human mab21l2. The characteristic expression pattern of mab21l2 facilitates a detailed description of the morphogenesis of the eyes and midbrain in the zebrafish. In the eye field, mab21l2 expression illustrates the transformation of the eye field to form two separate eyes in the anterior neural plate. Mab21l2 staining in the cyclopic mutants, cyc and oep, exhibited incomplete splitting of the eye primodium. In the midbrain, mab21l2 is expressed in the tectum, and its expression follows the expansion of the tectal region. In mutants affecting the mid-hindbrain boundary (MHB), mab21l2 expression is affected differentially. In the noi/pax2.1 mutant, mab21l2 is down-regulated and the size of the tectum remains small, whereas in the ace/fgf8 mutant, mab21l2 expression persists although the shape of the tectum is altered.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kudoh
authors.forename: I B
authors.initials: IB
authors.lastname: Dawid
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002970:Cleavage Stage, Ovum; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D005123:Eye / Q000196:embryology*; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D008636:Mesencephalon / Q000196:embryology*; D008969:Molecular Sequence Data; D051762:PAX2 Transcription Factor; D017386:Sequence Homology, Amino Acid; D019170:Somites; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000502:physiology; D015027:Zebrafish / Q000196:embryology / Q000235:genetics; D029961:Zebrafish Proteins
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051762:PAX2 Transcription Factor; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins; C512832:ndr2 protein, zebrafish; C497320:pax2a protein, zebrafish; C110484:tdgf1 protein, zebrafish