title: Change of migration time and separation window accompanied by field-enhanced sample stacking in capillary zone electrophoresis.
abstract: When field-enhanced sample stacking was used in capillary zone electrophoresis (CZE) analysis of cations, the decrease of migration time and the reduction of separation window was observed with increase of sample plug length. A simple equation expressing the migration velocity in the stacking process was derived to explain the above phenomenon. From experiments and theoretical consideration, we confirmed that this effect was caused by the higher potential gradient and larger eletroosmotic flow (EOF) mobility at the sample plug than those at the supporting electrolyte. A mathematical model appropriate for the computer simulation of such a system was studied considering the experimental results, and it was concluded that electroosmotic velocity (v(eof)) should be introduced to the equation of continuity as a constant.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, Higashi-hiroshima, Japan. hiro77@hiroshima-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Hirokawa
authors.forename: N
authors.initials: N
authors.lastname: Ikuta
authors.forename: T
authors.initials: T
authors.lastname: Yoshiyama
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
mesh_terms: D002412:Cations / Q000302:isolation & purification; D003198:Computer Simulation; D019075:Electrophoresis, Capillary / Q000379:methods*; D008432:Mathematical Computing; D008958:Models, Molecular; D013997:Time Factors
chemical_list: D002412:Cations