Using ATP bioluminescence technique for monitoring microbial activity in sludge.
This study demonstrates, for the first time, that the presence of suspended solids in waste-activated sludge interferes with adenosine triphosphate (ATP) bioluminescence tests. The sludge subject to acid/alkaline treatment represented the test sample. Without consideration of the effect of solid concentrations, one would erroneously estimate the density levels of heterotrophic bacteria in the sludge using ATP data. A light blockage model was proposed to evaluate the luminescence reading without the interference of suspended solids.
Chu
C P
CP
Chemical Engineering Department, National Taiwan University, Taipei 106, Taiwan.
Lee
D J
DJ
Chang
B V
BV
Liao
C S
CS
D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism; D018533:Biomass; D049454:Firefly Luciferin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements; D008433:Mathematics; D008828:Microbiological Techniques / Q000379:methods*; D012722:Sewage / Q000382:microbiology*
D012722:Sewage; D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases