title: Penicillin acylase purification with the aid of pseudo-affinity chromatography.
abstract: The introduction of affinity chromatography has opened a new dimension in protein purification. This article reviews the current techniques used in the penicillin acylase purification process, especially pseudo-affinity chromatography. A profile for a suitable ligand is established. An aromatic ring and the presence of one or several amino groups seem essential for proper interaction. Immobilized metal affinity chromatography now seems to be a good competitor.
authors.affiliation: Ecole Supérieure de Technologie des Biomolécules de Bordeaux, UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Fitton
authors.forename: N
authors.initials: N
authors.lastname: Verdoni
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.forename: X
authors.initials: X
authors.lastname: Santarelli
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D004800:Enzymes, Immobilized / Q000302:isolation & purification; D008024:Ligands; D010399:Penicillin Amidase / Q000302:isolation & purification*
chemical_list: D004800:Enzymes, Immobilized; D008024:Ligands; D010399:Penicillin Amidase