Application of molecular biology to impotence research.
To encourage further application of molecular biology in impotence research, we have compiled a list of techniques that have been or can be used in such endeavors. While by no means complete or perfect, the list encompasses both some of the most commonly used (such as RT-PCR) and some of the most promising (such as gene chip) methods. All three levels of the gene expression hierarchy, namely, DNA, RNA, and protein, are represented in the discussion. Whenever possible, each technique is discussed with references relevant to impotence research. Interested readers therefore can trace the original or the most recent research protocols for more detailed information.
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D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics / Q000378:metabolism; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D018983:DNA Footprinting; D015180:Electrophoresis, Gel, Two-Dimensional; D007172:Erectile Dysfunction / Q000503:physiopathology*; D006801:Humans; D015151:Immunoblotting; D017403:In Situ Hybridization; D008297:Male; D008967:Molecular Biology / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D020411:Oligonucleotide Array Sequence Analysis; D010413:Penis / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human