title: CD44-dependent lymphoma cell dissemination: a cell surface CD44 variant, rather than standard CD44, supports in vitro lymphoma cell rolling on hyaluronic acid substrate and its in vivo accumulation in the peripheral lymph nodes.
abstract: Cell motility is an essential element of tumor dissemination, allowing organ infiltration by cancer cells. Using mouse LB lymphoma cells transfected with standard CD44 (CD44s) cDNA (LB-TRs cells) or with the alternatively spliced CD44 variant CD44v4-v10 (CD44v) cDNA (LB-TRv cells), we explored their CD44-dependent cell migration. LB-TRv cells, but not LB-TRs or parental LB cells, bound soluble hyaluronic acid (HA) and other glycosaminoglycans (GAGs), and exclusively formed, under physiological shear force, rolling attachments on HA substrate. Furthermore, LB-TRv cells, but not LB-TRs cells or their parental LB cells, displayed accelerated local tumor formation and enhanced accumulation in the peripheral lymph nodes after s.c. inoculation. The aggressive metastatic behavior of i.v.-injected LB-TRV cells, when compared with that of other LB-transfectants, is attributed to more efficient migration to the lymph nodes, rather than to local growth in the lymph node. Injection of anti-CD44 monoclonal antibody or of the enzyme hyaluronidase also prevented tumor growth in lymph nodes of BALB/c mice inoculated with LB-TRv cells. The enhanced in vitro rolling and enhanced in vivo local tumor growth and lymph node invasion disappeared in LB cells transfected with CD44v cDNA bearing a point mutation at the HA binding site, located at the distal end of the molecule constant region. These findings show that the interaction of cell surface CD44v with HA promotes cell migration both in vitro and in vivo, and they contribute to our understanding of the mechanism of cell trafficking, including tumor spread.
authors.affiliation: The Lautenberg Center for General and Tumor Immunology, The Hebrew University-Hadassah Medical School, Jerusalem, 91120 Israel.
authors.forename: S B
authors.initials: SB
authors.lastname: Wallach-Dayan
authors.forename: V
authors.initials: V
authors.lastname: Grabovsky
authors.forename: J
authors.initials: J
authors.lastname: Moll
authors.forename: J
authors.initials: J
authors.lastname: Sleeman
authors.forename: P
authors.initials: P
authors.lastname: Herrlich
authors.forename: R
authors.initials: R
authors.lastname: Alon
authors.forename: D
authors.initials: D
authors.lastname: Naor
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D017398:Alternative Splicing; D000818:Animals; D000954:Antigens, Surface / Q000502:physiology; D001665:Binding Sites / Q000235:genetics; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D018076:DNA, Complementary; D005260:Female; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D016399:Lymphoma, T-Cell; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016296:Mutagenesis / Q000502:physiology; D013314:Stress, Mechanical; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D018076:DNA, Complementary; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid