title: Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate.
abstract: Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and gamma-glutamyl transpeptidase (gamma-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.
authors.affiliation: Institute of Aquaculture Torre de la Sal (CSIC), Castellón, Spain. samuel@iats.csic.es
authors.forename: S
authors.initials: S
authors.lastname: Peña-Llopis
authors.forename: J B
authors.initials: JB
authors.lastname: Peña
authors.forename: E
authors.initials: E
authors.lastname: Sancho
authors.forename: C
authors.initials: C
authors.lastname: Fernández-Vega
authors.forename: M D
authors.initials: MD
authors.lastname: Ferrando
mesh_terms: D000811:Anguilla / Q000502:physiology*; D000818:Animals; D001381:Azepines / Q000494:pharmacology* / Q000633:toxicity; D002219:Carbamates; D004351:Drug Resistance; D005978:Glutathione / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology* / Q000633:toxicity; D008099:Liver / Q000201:enzymology; D018482:Muscle, Skeletal / Q000201:enzymology; D013859:Thiocarbamates; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
chemical_list: D001381:Azepines; D002219:Carbamates; D006540:Herbicides; D013859:Thiocarbamates; C009821:molinate; D005980:Glutathione Reductase; D005723:gamma-Glutamyltransferase; D005978:Glutathione