Effect of vaginally administered (Ala(8,13,18))-magainin II amide on the morphology of implantation stage endometrium in the rhesus monkey (Macaca mulatta).
Intravaginal administration of an anti-microbial agent, (Ala(8,13,18))-magainin II amide, during blastocyst implantation inhibits pregnancy establishment in a dose-related manner in the rhesus monkey (Macaca mulatta). In the present study, mated female rhesus monkeys were vaginally inserted with tampons containing vehicle (Group 1; n = 5) and test agent (magainin, 0.5 mg/animal; Group 2; n = 6) on cycle day 20. Endometrial tissue samples were collected on Cycle Day 24 from all monkeys and processed for morphometric and ultrastructural analysis. Concentrations of estradiol-17beta, progesterone, and chorionic gonadotrophin in peripheral circulation were determined, which revealed that two monkeys in Group 1 were pregnant while no animals were pregnant in Group 2. Endometrial morphology, however, revealed histologic evidence of pregnancy in three out of the six magainin-treated animals. It appears that intra-vaginal administration of magainin II amide had a marginal effect on the implantation stage endometrium and the initiation of the implantation process in the rhesus monkey.
Ghosh
D
D
Department of Physiology, All India Institute of Medical Sciences, New Delhi 110029, India. dghosh_57@hotmail.com
Dhawan
L
L
Lalitkumar
P G
PG
Wong
V
V
Rosario
J F
JF
Hendrickx
A G
AG
Lasley
B L
BL
Overstreet
J W
JW
Sengupta
J
J
D000282:Administration, Intravaginal; D020533:Angiogenesis Inhibitors / Q000008:administration & dosage; D000818:Animals; D000890:Anti-Infective Agents / Q000009:adverse effects*; D023181:Antimicrobial Cationic Peptides; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology*; D004717:Endometrium / Q000033:anatomy & histology* / Q000098:blood supply / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000648:ultrastructure; D004847:Epithelial Cells / Q000648:ultrastructure; D005260:Female; D008253:Macaca mulatta; D008297:Male; D008854:Microscopy, Electron; D010455:Peptides / Q000008:administration & dosage; D011247:Pregnancy
D020533:Angiogenesis Inhibitors; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C415246:alanine(8,13,18)-magainin 2 amide