Identity of Naegleria strains isolated from organs of freshwater fishes.
Eighteen Naegleria strains were isolated from organs of freshwater fishes belonging to 5 species. Morphometric study allowed the separation of the Naegleria strains from the non-vahlkampfiid amoeboflagellates, but was inadequate for species determination. Six strains, representatives of groups that had a slightly different cyst size, were selected and corresponding derived clones were subjected to sequence analysis and riboprinting restriction fragment length polymorphism (RFLP)-PCR analysis of the small subunit (SSU) rRNA genes. One strain isolated from the brain of a fish with systemic infection was characterised by an intronless 2 kb long SSU rRNA gene and was identified as N. australiensis. Another 5 strains had a 1.3 kb long group I intron in their SSU rRNA gene and, based on the SSU rRNA sequences and riboprints, RFLP-PCR patterns appeared in phylogenetic trees to be closely related to Naegleria clarki.
Dyková
I
I
Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceské Budejovice. iva@paru.cas.cz
Kyselová
I
I
Pecková
H
H
Oborník
M
M
Lukes
J
J
D000562:Amebiasis / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes; D005618:Fresh Water; D008969:Molecular Sequence Data; D009253:Naegleria / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016053:RNA, Protozoan / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D021521:Ribotyping
D016053:RNA, Protozoan; D012335:RNA, Ribosomal