Report from the 1st International NOD Mouse T-Cell Workshop and the follow-up mini-workshop.
A workshop on autoreactive T-cell responses in NOD mice was held to optimize autoreactive T-cell detection methodologies. Using different proliferation assay protocols, 1 of the 11 participating laboratories detected spontaneous T-cell responses to GAD(524-543) and insulin(9-23) in their NOD mice. Two other laboratories were able to detect autoreactive responses when using enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) analysis of cytokines in culture supernatants, suggesting that these assays provided greater sensitivity. To address the divergent findings, a follow-up mini-workshop tested NOD mice from four different colonies side-by-side for T-cell proliferative responses to an expanded panel of autoantigens, using the protocol that had enabled detection of responses in the 1st International NOD Mouse T-Cell Workshop. Under these assay conditions, 16 of 16 NOD mice displayed proliferative responses to whole GAD65, 13 of 16 to GAD(524-543), 9 of 16 to GAD(217-236), 7 of 16 to insulin(9-23), and 5 of 16 to HSP277. Thus, spontaneous proliferative T-cell responses can be consistently detected to some beta-cell autoantigens and peptides thereof. Overall, the results suggest that more sensitive assays (e.g., ELISPOT, ELISA analysis of cytokines in supernatants, or tetramer staining) may be preferred for the detection of autoreactive T-cells.
Kaufman
D L
DL
Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California 90095-1735, USA. dkaufman@mednet.ucla.edu
Tisch
R
R
Sarvetnick
N
N
Chatenoud
L
L
Harrison
L C
LC
Haskins
K
K
Quinn
A
A
Sercarz
E
E
Singh
B
B
von Herrath
M
M
Wegmann
D
D
Wen
L
L
Zekzer
D
D
D000818:Animals; D001324:Autoantigens / Q000494:pharmacology; D015551:Autoimmunity; D002455:Cell Division / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005968:Glutamate Decarboxylase / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000494:pharmacology; D007527:Isoenzymes / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD / Q000276:immunology*; D010446:Peptide Fragments / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D001324:Autoantigens; C439757:HSP277 protein, mouse; D006360:Heat-Shock Proteins; D007328:Insulin; D007527:Isoenzymes; D010446:Peptide Fragments; C097877:insulin B (9-23); C439756:GAD65 (217-236); C439755:GAD65 (524-543); D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2