Lipopolysaccharide-induced impairment of classical swine fever virus infection in monocytic cells is sensitive to 2-aminopurine.
Lipopolysaccharide (LPS) impairs classical swine fever virus (CSFV) replication in monocytic cells, which are primary targets for CSFV and mediators of virus-induced immunomodulation. Although soluble antiviral factors including interferons (IFN) were not detected, IFN-alpha and IFN-beta mRNA were induced. The serine threonine protein kinase inhibitor 2-aminopurine, impeded this antiviral activity. These results indicate that the LPS-induced antiviral state employs signaling pathways, in which the double-stranded RNA-dependent protein kinase (PKR) is actively involved.
Knoetig
Sonja M
SM
Institute of Virology and Immunoprophylaxis, Sensemattstrasse 293, 3147, Mittelhäusern, Switzerland.
McCullough
Kenneth C
KC
Summerfield
Artur
A
D015075:2-Aminopurine / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D006691:Classical Swine Fever / Q000821:virology; D006692:Classical Swine Fever Virus / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000821:virology; D009000:Monocytes / Q000276:immunology / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects*
D016207:Cytokines; D008070:Lipopolysaccharides; D015075:2-Aminopurine; D007372:Interferons