title: Pax6 controls the expression of Lewis x epitope in the embryonic forebrain by regulating alpha 1,3-fucosyltransferase IX expression.
abstract: Pax6 is a transcription factor involved in brain patterning and neurogenesis. Expression of Pax6 is specifically observed in the developing cerebral cortex, where Lewis x epitope that is thought to play important roles in cell interactions is colocalized. Here we examined whether Pax6 regulates localization of Lewis x using Pax6 mutant rat embryos. The Lewis x epitope disappeared in the Pax6 mutant cortex, and activity of alpha1,3-fucosyltransferase, which catalyzed the last step of Lewis x biosynthesis, drastically decreased in the mutant cortex as compared with the wild type. Furthermore, expression of a fucosyltransferase gene, FucT-IX, specifically decreased in the mutant, while no change was seen for expression of another fucosyltransferase gene, FucT-IV. These results strongly suggest that Pax6 controls Lewis x expression in the embryonic brain by regulating FucT-IX gene expression.
authors.affiliation: Department of Biochemical Cell Research, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Youichi
authors.initials: Y
authors.lastname: Tajima
authors.forename: Taka
authors.initials: T
authors.lastname: Osanai
authors.forename: Asao
authors.initials: A
authors.lastname: Katsume
authors.forename: Michinori
authors.initials: M
authors.lastname: Kohara
authors.forename: Takashi
authors.initials: T
authors.lastname: Kudo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Noriko
authors.initials: N
authors.lastname: Takashima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ishii
authors.forename: Shun
authors.initials: S
authors.lastname: Nakamura
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Sanai
mesh_terms: D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D017931:DNA Primers; D000939:Epitopes / Q000235:genetics*; D005136:Eye Proteins; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology*; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000235:genetics*; D008969:Molecular Sequence Data; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000196:embryology / Q000201:enzymology / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins
chemical_list: D017931:DNA Primers; D000939:Epitopes; D005136:Eye Proteins; D018398:Homeodomain Proteins; D016256:Lewis X Antigen; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605733:Pax6 protein, rat; D012097:Repressor Proteins; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase