Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.
By using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of Listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlB gene. Strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups.
Unnerstad
H
H
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden. Helle.Unnerstad@sva.se
Ericsson
H
H
Alderborn
A
A
Tham
W
W
Danielsson-Tham
M L
ML
Mattsson
J G
JG
D000818:Animals; D001426:Bacterial Proteins; D015139:Blotting, Southern; D011756:Diphosphates / Q000378:metabolism*; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013698:Templates, Genetic
D001426:Bacterial Proteins; D011756:Diphosphates; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes