Penicillin acylase purification with the aid of pseudo-affinity chromatography.
The introduction of affinity chromatography has opened a new dimension in protein purification. This article reviews the current techniques used in the penicillin acylase purification process, especially pseudo-affinity chromatography. A profile for a suitable ligand is established. An aromatic ring and the presence of one or several amino groups seem essential for proper interaction. Immobilized metal affinity chromatography now seems to be a good competitor.
Fitton
V
V
Ecole Supérieure de Technologie des Biomolécules de Bordeaux, UMR 5544, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France.
Verdoni
N
N
Sanchez
J
J
Santarelli
X
X
D002846:Chromatography, Affinity / Q000379:methods; D004800:Enzymes, Immobilized / Q000302:isolation & purification; D008024:Ligands; D010399:Penicillin Amidase / Q000302:isolation & purification*
D004800:Enzymes, Immobilized; D008024:Ligands; D010399:Penicillin Amidase