Crystallization and preliminary X-ray analysis of a Trx domain of human thioredoxin-like protein.
The Trx domain of human thioredoxin-like protein has been purified and crystallized using ammonium sulfate as precipitant. The crystal belongs to space group C2, with unit-cell parameters a = 87.5, b = 48.5, c = 29.8 A, beta = 99.59 degrees. It has one molecule per asymmetric unit and diffracts beyond 2.2 A under cryoconditions (100 K) using an in-house Cu rotating-anode X-ray generator.
Chen
X
X
Laboratory of Structural Biology, Department of Biological Science and Technology, Tsinghua University, Beijing 100084, People's Republic of China.
Jin
J
J
Gao
Y
Y
Guo
Q
Q
Sun
Y
Y
Tang
H
H
Yuan
J
J
Qiang
B
B
Rao
Z
Z
D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013879:Thioredoxins / Q000737:chemistry* / Q000302:isolation & purification
C504766:TXNL1 protein, human; D013879:Thioredoxins