Identification of a BIBAC clone that co-segregates with the petunia restorer of fertility (Rf) gene.
Molecular markers closely linked to the Restorer of fertility (Rf) locus in petunia were sought by conducting a bulk segregant analysis. The co-segregation of markers and Rf was tested on a large BC1 population produced from two different parental lines carrying Rf. The recombination frequency between OP704 and ECCA/MACT, the two most distal markers utilized in the fine-scale mapping. was significantly different in populations derived from parents that carry different nuclear backgrounds. The fine mapping identified an amplified fragment length polymorphism (AFLP) marker that co-segregates with Rf. A petunia BIBAC library (four genome equivalents), with an average insert size of 70 kb, was constructed and screened with the linked marker. A contiguous map was constructed from three different BIBAC clones that hybridized to the marker. As a result, we have identified a 37.5-kb BIBAC clone that co-segregates with Rf.
Bentolila
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Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Hanson
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MR
D002874:Chromosome Mapping; D020090:Chromosome Segregation; D022202:Chromosomes, Artificial, Bacterial; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D018744:DNA, Plant; D005819:Genetic Markers