Chemical and biological strategies for engineering cell surface glycosylation.
Oligosaccharides play a crucial role in many of the recognition, signaling, and adhesion events that take place at the surface of cells. Abnormalities in the synthesis or presentation of these carbohydrates can lead to misfolded and inactive proteins, as well as to several debilitating disease states. However, their diverse structures, which are the key to their function, have hampered studies by biologists and chemists alike. This review presents an overview of techniques for examining and manipulating cell surface oligosaccharides through genetic, enzymatic, and chemical strategies.
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Department of Chemistry, University of California, Berkeley, California 94720, USA. bertozzi@cchem.berkeley.edu
Bertozzi
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D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D006001:Glycoconjugates / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering; D017510:Protein Folding
D000939:Epitopes; D006001:Glycoconjugates; D009844:Oligosaccharides; D016695:Glycosyltransferases; D006026:Glycoside Hydrolases