title: Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions.
abstract: We developed a visible detection system for antibody-catalyzed retro-aldol-retro-Michael reactions. Aldolase antibody 38C2 catalyzed the reaction of substrate 1 to provide 6-bromo-2-napthol that forms a visible colored azo dye with diazonium salts. This system has potential for the screening of novel catalysts.
authors.affiliation: Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: F
authors.initials: F
authors.lastname: Tanaka
authors.forename: L
authors.initials: L
authors.lastname: Kerwin
authors.forename: D
authors.initials: D
authors.lastname: Kubitz
authors.forename: R A
authors.initials: RA
authors.lastname: Lerner
authors.forename: C F
authors.initials: CF
authors.lastname: Barbas
mesh_terms: D000818:Animals; D017151:Antibodies, Catalytic / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D001711:Biotransformation; D002384:Catalysis; D003979:Diazonium Compounds; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D006825:Hybridomas / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D009284:Naphthols / Q000737:chemistry
chemical_list: D017151:Antibodies, Catalytic; D003979:Diazonium Compounds; D009284:Naphthols; C034667:6-bromo-2-naphthol; D005634:Fructose-Bisphosphate Aldolase