title: The effects of mesenteric ischemia on ileal colonization, intestinal integrity, and bacterial translocation in newborn piglets.
abstract: The effects of mesenteric ischemia on ileal colonization, intestinal integrity, and bacterial translocation (BT) in newborn piglets were investigated in 362-day-old Pietrain piglets. Group I, controls were not operated upon; group II underwent a sham laparotomy; and group III underwent ligation of the mesenteric vessels in the distal ileum. After 3 days, the kidneys, spleens, livers, and ileal segments were harvested for microbial and histologic analyses. Two piglets in the ischemic group died; microscopic examination showed severe histologic lesions of the ischemic area. Escherichia coli counts were increased in the ischemic segment compared to the upper loop (P < 0.05). Ischemia favoured staphylococcal colonization, whereas in the sham group a drastic reduction of these organisms was observed (P < 0.005). BT to the kidneys, spleen, and liver occurred normally in the control group. Ischemia significantly increased the total microflora in the spleen and liver (P < 0.05) and furthered dissemination of Clostridium perfringens in the kidneys (P < 0.05); 50% of ischemic animals had proteolytic clostridia in this organ (P < 0.05). Moreover, the incidence of E. coli in the kidneys, spleen, and liver was significantly higher in the sham and ischemic groups than in the controls (P < 0.05). Ileal ischemia thus induced significant histologic lesions, and surgery rather than gut microflora controls translocation.
authors.affiliation: Unité Mère-Enfant, Services de Pédiatrie II et de Chirurgie infantile, CHU Nord, Amiens, France.
authors.forename: A T
authors.initials: AT
authors.lastname: Meddah
authors.forename: L
authors.initials: L
authors.lastname: Leke
authors.forename: M B
authors.initials: MB
authors.lastname: Romond
authors.forename: E
authors.initials: E
authors.lastname: Grenier
authors.forename: C
authors.initials: C
authors.lastname: Cordonnier
authors.forename: B
authors.initials: B
authors.lastname: Risbourg
authors.forename: J P
authors.initials: JP
authors.lastname: Canarelli
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D018988:Bacterial Translocation; D003013:Clostridium / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007082:Ileum / Q000098:blood supply*; D007511:Ischemia / Q000382:microbiology*; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008297:Male; D008643:Mesentery / Q000098:blood supply*; D023421:Models, Animal; D013154:Spleen / Q000382:microbiology; D013552:Swine