Detection of the Mr 110,000 lung resistance-related protein LRP/MVP with monoclonal antibodies.
The Mr 110,000 lung resistance-related protein (LRP), also termed the major vault protein (MVP), constitutes >70% of subcellular ribonucleoprotein particles called vaults. Overexpression of LRP/MVP and vaults has been linked directly to MDR in cancer cells. Clinically, LRP/MVP expression can be of value to predict response to chemotherapy and prognosis. Monoclonal antibodies (MAbs) against LRP/MVP have played a critical role in determining the relevance of this protein in clinical drug resistance. We compared the applicability of the previously described MAbs LRP-56, LMR-5, LRP, 1027, 1032, and newly isolated MAbs MVP-9, MVP-16, MVP-18, and MVP-37 for the immunodetection of LRP/MVP by immunoblotting analysis and by immunocyto- and histochemistry. The availability of a broader panel of reagents for the specific and sensitive immunodetection of LRP/MVP should greatly facilitate biological and clinical studies of vault-related MDR.
Schroeijers
A B
AB
Department of Pathology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands.
Scheffer
G L
GL
Reurs
A W
AW
Pijnenborg
A C
AC
Abbondanza
C
C
Wiemer
E A
EA
Scheper
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung; D018288:Carcinoma, Small Cell; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005404:Fixatives; D005557:Formaldehyde; D007150:Immunohistochemistry; D008175:Lung Neoplasms; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D016612:Paraffin Embedding; D016707:Tissue Fixation; D014407:Tumor Cells, Cultured; D020394:Vault Ribonucleoprotein Particles / Q000276:immunology / Q000378:metabolism*; D000099187:Major Vault Protein
D000911:Antibodies, Monoclonal; D005404:Fixatives; D005557:Formaldehyde; D009363:Neoplasm Proteins; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein