title: Lymphangiogenesis quantification using quantitative PCR and breast cancer as a model.
abstract: The detection of lymphangiogenesis (formation of new lymphatics) has previously been difficult to measure, primarily due to the lack of specific markers for lymphatic endothelium. Using conventional PCR (polymerase chain reaction), DNA sequencing, plasmid synthesis, and real-time quantitative PCR (RTQPCR), we report a new approach to enable the measurement of lymphangiogenesis using LYVE-1, a novel, specific lymphatic marker in breast cancer tissue. By using a Scorpion-based probe system with the RTQPCR analyser, a highly sensitive and specific detection and quantitation of LYVE-1 was possible. It was found that lymphangiogenesis occurred in all breast specimens and that higher levels were found in tumours which had spread to the lymph nodes.
authors.affiliation: Metastasis Research Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, United Kingdom. giles@stbartholomews.com
authors.forename: G H
authors.initials: GH
authors.lastname: Cunnick
authors.forename: W G
authors.initials: WG
authors.lastname: Jiang
authors.forename: K F
authors.initials: KF
authors.lastname: Gomez
authors.forename: R E
authors.initials: RE
authors.lastname: Mansel
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001943:Breast Neoplasms / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000254:growth & development / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008198:Lymph Nodes / Q000254:growth & development* / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000276:immunology / Q000473:pathology; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D033921:Vesicular Transport Proteins
chemical_list: D015415:Biomarkers; D006023:Glycoproteins; C119248:LYVE1 protein, human; D012333:RNA, Messenger; D033921:Vesicular Transport Proteins