title: Detection of amp C in Enterobacter cloacae in China.
abstract: PCR amplification of 55 strains of Enterobacter cloacae indicated 51 of them had amp C structural gene verified by DNA sequence and Southern blotting. All PCR products were cleaved into 666- and 328-bp fragments by Kpn1 restriction enzyme. Imipenem was the most potent inducer for mRNA expression of amp C gene and beta-lactamase activity. The beta-Lactamase inhibitor R0481220 strongly inhibited Amp C beta-lactamases; 96.4% (53/55) of Enterobacter cloacae producing Amp C enzyme were susceptible to cefepime.
authors.affiliation: Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100083, People's Republic of China. zhengyl@163bj.com
authors.forename: Y L
authors.initials: YL
authors.lastname: Zhang
authors.forename: J T
authors.initials: JT
authors.lastname: Li
authors.forename: M W
authors.initials: MW
authors.lastname: Zhao
mesh_terms: D001426:Bacterial Proteins; D001483:Base Sequence; D015139:Blotting, Southern; D002681:China; D016972:Enterobacter cloacae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005798:Genes, Bacterial / Q000235:genetics; D006868:Hydrolysis / Q000187:drug effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; C048795:AmpC beta-lactamases; D001618:beta-Lactamases