Characterization of histamine-induced increases in intracellular free Ca2+ concentrations in Chang liver cells.
The effect of histamine on intracellular free Ca2+ levels ([Ca2+]i) in Chang liver cells were investigated by using fura-2 as a Ca2+ dye. Histamine (0.2-50 microM) increased [Ca2+]i in a concentration-dependent manner with an EC50 value of 0.8 microM. The [Ca2+]i response comprised an initial rise, a slow decay, and a sustained phase. Extracellular Ca2+ removal inhibited 50% of the maximum [Ca2+]i signal and abolished the sustained phase. After pretreatment with 5 microM histamine in Ca2+-free medium for 4 min, addition of 3 mM Ca2+ induced a [Ca2+]i increase with a magnitude 7-fold greater than control. In Ca2+-free medium, after treatment with 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor), 5 microM histamine failed to increase [Ca2+]i. Histamine (5 microM)-induced intracellular Ca2+ release was abolished
Cheng
J S
JS
Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Lee
K C
KC
Wang
J L
JL
Chang
H T
HT
Chou
K J
KJ
Tang
K Y
KY
Jan
C R
CR
D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002460:Cell Line; D002927:Cimetidine / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes; D016257:Fura-2; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D011738:Pyrilamine / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000378:metabolism; D011970:Receptors, Histamine H2 / Q000187:drug effects / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D019284:Thapsigargin / Q000494:pharmacology
D005456:Fluorescent Dyes; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D019284:Thapsigargin; D002927:Cimetidine; D006632:Histamine; D015544:Inositol 1,4,5-Trisphosphate; D000252:Calcium-Transporting ATPases; D011738:Pyrilamine; D002118:Calcium; D016257:Fura-2