[Dominant selecting culture medium of Peucedanum decursivum by orthogonal design].
OBJECTIVE
To select the optimum medium for callus culture of Peucedanum decursivum.


METHODS
Orthogonal design was applied in determination of hormonal kinds and concentration, and qualitative analysis of callus was done initially.


RESULTS
The MS basic medium with 1 mg/L 2,4-D was the best one; coumarin was detected in callus preliminarily.


CONCLUSIONS
It was effective that orthogonal design applied in screening medium; feasible foundation was offered for Peucedanum decursivum cell culture producing active ingredient.
Peng
F
F
Hunan College of Traditional Chinese Medicine, Changsha 410004.
Ling
H
H
Hu
Y
Y
D019661:Apiaceae / Q000737:chemistry / Q000254:growth & development*; D003374:Coumarins / Q000032:analysis; D003470:Culture Media; D046508:Culture Techniques; D006728:Hormones / Q000032:analysis
D003374:Coumarins; D003470:Culture Media; D006728:Hormones; C030123:coumarin