[Optimization of culture conditions of Aspergillus niger for the synthesis of alpha-N-acetylgalactosaminidase and alpha-galactosidase].
Different factors have been investigated for their effect on the process of biosynthesis of alpha-N-acetylgalactosaminidase and alpha-galactosidase of Aspergillus niger under deep-water cultivation. Optimal sources and concentrations of carbon (soy flour--25 g/l) and nitrogen (pepton--7.5 g/l) were estimated. It has been established that temperature of 25 degrees C, pH 6.0, growing in 50 ml medium at the swing velocity 220 rev/min. for 6-8 days are optimal cultivation parameters. It has been shown that dry borine blood (in concentration of 1%) and a number of guanidine derivatives (guanidine carbonate--0.25%, nitroaminoguanison dimethylaminobenzaldehyde--0.05%, nitroaminoguanison of salicylic aldehyde--0.1%) can play the part of inducers of the mentioned glycosidase synthesis. When growing fungal culture in selected conditions synthesis of enzymes increased almost 3 times. Activity of alpha-N-acetylgalactosaminidase was 0.46 E/ml, and alpha-galactosidase--1.9 E/ml.
Borzova
N V
NV
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Malanchuk
V M
VM
Varbanets
L D
LD
Seĭfullina
I I
II
Zubkov
S V
SV
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D001769:Blood / Q000378:metabolism; D002417:Cattle; D003470:Culture Media; D019791:Guanidine / Q000031:analogs & derivatives / Q000378:metabolism; D006596:Hexosaminidases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D000519:alpha-Galactosidase / Q000378:metabolism*; D048809:alpha-N-Acetylgalactosaminidase
D003470:Culture Media; D006596:Hexosaminidases; D000519:alpha-Galactosidase; D048809:alpha-N-Acetylgalactosaminidase; D019791:Guanidine