title: Flow injection analysis with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS).
abstract: Studies on the capabilities of flow injection analysis (FIA) combined with on-line characterisation of model compounds via a combination of diode array UV, 1H-NMR, FT-IR spectroscopy and mass spectrometry are described. Using this combination of spectrometers enabled the on-flow collection of UV, 1H-NMR, IR and MS for a range of model compounds. Samples were introduced into the system as solutions in deuterium oxide in concentrations ranging from 1.4 to 8.4 mg ml(-1). A sample volume of 100 microl was used for FIA at a flow rate of 1 ml min(-1). From these studies a practical working quantity of ca. 140 microg/sample of analyte was determined which provided characteristic spectra.
authors.affiliation: AstraZeneca Pharmaceuticals, Drug Metabolism & Pharmacokinetics Department, Mereside, Alderley Park, Macclesfield, SK10 4TG, Cheshire, UK.
authors.forename: Eva
authors.initials: E
authors.lastname: Lenz
authors.forename: Steve
authors.initials: S
authors.lastname: Taylor
authors.forename: Craig
authors.initials: C
authors.lastname: Collins
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
authors.forename: Dave
authors.initials: D
authors.lastname: Louden
authors.forename: Alan
authors.initials: A
authors.lastname: Handley
mesh_terms: D010129:4-Aminobenzoic Acid / Q000032:analysis; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D010129:4-Aminobenzoic Acid