Alteration of integrins by interleukin-1alpha in human pancreatic cancer cells.
BACKGROUND
Adhesion of tumor cells to extracellular matrix (ECM) proteins plays an important role in tumor invasion and metastasis.


OBJECTIVE
To investigate the expression of integrins in human pancreatic cancer cell lines and its alteration by interleukin (IL)-1alpha to examine the mechanism of adhesion of metastatic human pancreatic cancer cells to ECM proteins.


METHODS
The expression of integrin subunits and their alteration by IL-1alpha were examined by flow-cytometric analysis and cellular enzyme-linked immunosorbent assay in three metastatic human pancreatic cancer cell lines (AsPC-1, BxPC-3, and SW1990) and two nonmetastatic cancer cell lines (PaCa-2 and PANC-1). In addition, assays of cancer cell adhesion to ECM proteins were performed to investigate if increased integrin expression actually affected the adhesive interaction between cancer cells and the putative integrin ECM ligands.


RESULTS
The alpha(6) subunit expressed in metastatic cancer cells was enhanced by IL-1alpha. Metastatic cancer cells also showed preferential adherence to laminin compared with nonmetastatic cancer cells, and this was enhanced by IL-1alpha.


CONCLUSIONS
In pancreatic cancer, the enhancement of alpha(6)beta(1) integrin by IL-1alpha through IL-1 receptor type I, as well as the expression of alpha(6)beta(1) integrin, plays an important role in metastasis formation.
Sawai
H
H
First Department of Surgery, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 4678601, Japan.
Yamamoto
M
M
Okada
Y
Y
Sato
M
M
Akamo
Y
Y
Takeyama
H
H
Manabe
T
T
D015703:Antigens, CD / Q000032:analysis; D002448:Cell Adhesion; D024042:Collagen Type I / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D039482:Integrin alpha5; D039503:Integrin alpha6; D039121:Integrin alpha6beta1; D019012:Integrin beta1 / Q000032:analysis; D016023:Integrins / Q000032:analysis / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D009362:Neoplasm Metastasis; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D017472:Receptors, Interleukin-1 / Q000032:analysis; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D024042:Collagen Type I; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039482:Integrin alpha5; D039503:Integrin alpha6; D039121:Integrin alpha6beta1; D019012:Integrin beta1; D016023:Integrins; D007375:Interleukin-1; D007797:Laminin; D017472:Receptors, Interleukin-1