title: Fluorescence polarization: analysis of carbohydrate-protein interaction.
abstract: Fluorescence polarization has been widely used for the studies on the molecular motion in solution and has been applied to immunoassays for proteins, therapeutic drug monitoring in clinical pharmacy, and assays for environmentally toxic compounds. Because fluorescence polarization is most readily applicable to the kinetic analysis of the binding reaction between a substance having small molecular mass and a receptor molecule, this method is easily applied to the analysis of carbohydrate-lectin binding. In this tutorial Thematic Review, we briefly introduce the principles of fluorescence polarization and some applications of fluorescence polarization technique to glycobiology.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kinki University, Kowakae 3-4-1, Higashi-Osaka, 577, Japan. k_kakehi@phar.kindai.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kakehi
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: M
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D001236:Aspergillus oryzae / Q000737:chemistry; D001665:Binding Sites / Q000502:physiology; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D027622:Crocus / Q000737:chemistry; D005454:Fluorescence Polarization / Q000379:methods*; D007700:Kinetics; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D012278:Ricinus / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods; D015003:Yeasts / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D037102:Lectins; D037121:Plant Lectins; D011506:Proteins