Inhibition of gamma-irradiation induced adhesion molecules and NO production by alginate in human endothelial cells.
Inflammation is a frequent radiation-induced reaction following therapeutic irradiation. Treatment of human umbilical endothelial cells (HUVEC) with gamma-irradiation (gammaIR) induces the expression of adhesion proteins such as intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Since the upregulation of these proteins on endothelial cell surface has been known to be associated with inflammation, interfering with the expression of adhesion molecules is an important therapeutic target. In the present study, we demonstrate that high mannuronic acid-containing alginate (HMA) inhibits gammaIR induced expression of ICAM-1, VCAM-1, and E-selectin on HUVEC in a dose dependent manner. HMA also inhibited gammaIR induced production of Nitric oxide (NO). These data suggest that HMA has therapeutic potential for the treatment of various inflammatory disorder associated with an increase of endothelial leukocyte adhesion molecules.
Son
E W
EW
Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University, Suwon, Kyunggi-do 440-746 Korea.
Cho
C K
CK
Rhee
D K
DK
Pyo
S
S
D000464:Alginates / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000528:radiation effects*; D002460:Cell Line; D019040:E-Selectin / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005720:Gamma Rays; D006801:Humans; D007202:Indicators and Reagents; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D009569:Nitric Oxide / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects / Q000528:radiation effects; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D000464:Alginates; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007202:Indicators and Reagents; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide