title: Clinical utility of capillary polymerase chain reaction for diagnosis of Cytomegalovirus pneumonia.
abstract: The purpose of this retrospective study was to assess the diagnostic efficacy of CMV DNA detection by capillary PCR in patients with interstitial pneumonia. Of 882 samples taken from 363 patients, 317 were obtained from sputum, 94 from BAL fluid, 291 from blood and 180 from urine. PCR for CMV was positive in 58 samples (6.6%), with positive detection for 6.9% of sputum, 10.6% of BAL fluid, 4.1% of blood and 7.8% of urine samples. CMV pneumonia was diagnosed retrospectively in 34 (9.4%) of the 363 patients by demonstration of CMV antigen-positive cytomegalic inclusion bodies in lung tissue sections. The positive and negative predictive values were 100% (10/10) and 98.8% (83/84) for the BAL fluid samples and 95.5% (21/22) and 99.7% (294/295) for the sputum samples, respectively. Clinical sensitivity and specificity were 90.9% (10/11) and 100% (83/83) for the BAL fluid samples and 95.5% (21/22) and 99.7% (294/295) for the sputum samples, respectively. However, the blood and urine samples showed poor clinical sensitivity and low positive predictive values. We suggest that the use of capillary PCR for BAL fluid and sputum samples is very useful for diagnosing CMV pneumonia in patients with interstitial pneumonia in whom CMV pneumonia is suspected.
authors.affiliation: First Department of Internal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Honda
authors.forename: J
authors.initials: J
authors.lastname: Yonemitsu
authors.forename: H
authors.initials: H
authors.lastname: Kitajima
authors.forename: N
authors.initials: N
authors.lastname: Yosida
authors.forename: T
authors.initials: T
authors.lastname: Fumirori
authors.forename: K
authors.initials: K
authors.lastname: Oizumi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D002196:Capillaries; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011024:Pneumonia, Viral / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D016133:Polymerase Chain Reaction / Q000592:standards*; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012463:Saliva / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004279:DNA, Viral