Affinity purification of DNA-binding proteins.
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed. The purification strategies for transcription factors, restriction enzymes, telomerases, DNA and RNA polymerase and DNA binding antibodies are described. Different applications of DNA affinity chromatography are presented.
Gadgil
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Department of Biochemistry, University of Tennessee, 858 Madison Avenue, Memphis, TN 38163, USA.
Oak
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SA
Jarrett
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HW
D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification; D019098:Telomerase / Q000302:isolation & purification; D013698:Templates, Genetic; D014157:Transcription Factors / Q000302:isolation & purification
D017931:DNA Primers; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA; D019098:Telomerase; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase