Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid.
A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was developed. The thinly coated polymer was prepared by co-polymerizing N,N,N-trimethylaminoethyl methacrylate, 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate in the presence of p-vinylbenzeneboronic acid ester with sialic acid. The sensor showed a selective response to ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of ganglioside.
Kugimiya
A
A
Laboratory of Synthetic Biochemistry, Faculty of Information Sciences, Hiroshima City University, Asaminami-ku, Hiroshima 731-3194, Japan.
Takeuchi
T
T
D005677:G(M1) Ganglioside / Q000032:analysis / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D011108:Polymers / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D011108:Polymers; D005677:G(M1) Ganglioside; D019158:N-Acetylneuraminic Acid