Effects of bisphenol A and its derivatives on the response of GABA(A) receptors expressed in Xenopus oocytes.
To study the effects of bisphenol-A (BPA) known to have estrogenic actions, and its derivatives, 3,5-dimethylphenol (DMP) and p-t-butylphenol (TBP), on ionotropic gamma-aminobutyric acid (GABA) receptors, GABA(A) receptors were expressed in Xenopus oocytes by injecting both poly(A)+ RNA prepared from rat whole brain and cRNAs synthesized from cloned cDNAs of alpha1 and beta1 subunit of the bovine receptors, and their electrical responses were measured by the voltage clamping method. BPA caused the potentiation and inhibition of the former receptor-responses, while it caused only inhibition of the latter ones. In the presence of low concentrations of GABA, DMP and TBP potentiated the responses of both receptors. DMP and TBP also increased the rate of decay of the response, possibly by desensitization of the receptors when GABA solution was continuously bath-applied. Diethyl terephthalate (DTP), which is also known to have estrogenic actions, had little effect on both the responses and the decay of both receptors.
Aoshima
H
H
Department of Physics, Biology and Informatics, Faculty of Science, Yamaguchi University, Yoshida, Japan. aoshima@po.cc.yamaguchi-u.ac.jp
Hossain
S J
SJ
Imamura
H
H
Shingai
R
R
D000818:Animals; D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010636:Phenols / Q000494:pharmacology*; D010795:Phthalic Acids / Q000494:pharmacology; D011061:Poly A / Q000235:genetics; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis; D014992:Xylenes / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D010795:Phthalic Acids; D011061:Poly A; D011963:Receptors, GABA-A; D011994:Recombinant Proteins; D014992:Xylenes; D005680:gamma-Aminobutyric Acid; C453472:diethyl terephthalate; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C006862:butylphen; C016834:3,5-xylenol