Ecm11 is located in the cell nucleus throughout mitosis.
It was found previously that Ecm11 interacts with Cdc6 in a two-hybrid screen. To verify that these two gene products can actually encounter in the living cell, ECM11 was expressed as a fusion with GFP. The fusion protein was located in the cell nucleus throughout all stages of mitosis. The protein was detected in discrete clusters in the nucleus. Clusters may be due to Ecm11 protein polymerisation and association with discrete structures in the nucleus. Two Ecm 11 molecules interact in the two-hybrid assay, suggesting the presence of functional dimmers. Ecm11 does not promote transcription of beta-galactosidase reporter gene, when fused with LexA DNA binding domain and fetch to the promoter of the reporter gene. According to our observation, we suppose that Ecm11 is a nuclear protein involved in the upholding chromatin structure.
Comino
A
A
National Institute of Chemistry, Ljubljana, Slovenia.
Mancek
M
M
Komel
R
R
D018797:Cell Cycle Proteins / Q000032:analysis / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008938:Mitosis / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D020798:Two-Hybrid System Techniques
C066503:CDC6 protein, S cerevisiae; D018797:Cell Cycle Proteins; D002843:Chromatin; D005656:Fungal Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins