title: Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography.
abstract: Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected in Korea. The stationary phase used was Zorbax 300 SB C18 and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and E in stems were higher than those in roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.
authors.affiliation: College of Pharmacy, Chungnam National University, Taejon 305-764, Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Kang
authors.forename: P T
authors.initials: PT
authors.lastname: Linh
authors.forename: X F
authors.initials: XF
authors.lastname: Cai
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
mesh_terms: D027481:Araliaceae / Q000737:chemistry*; D001208:Asia; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D017705:Lignans; D013058:Mass Spectrometry; D010666:Phenylpropionates / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D012997:Solvents; C421885:eleutheroside E; C028305:syringin