Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii.
The distribution of and relationship between F-actin and G-actin were investigated in pollen grains and pollen tubes of Lilium davidii Duch. using a confocal laser scanning microscope after fluorescence and immunofluorescence labeling. Circular F-actin bundles were found to be the main form of microfilament cytoskeleton in pollen grains and pollen tubes. Consistent with cytoplasmic streaming in pollen tubes, there were no obvious F-actin bundles in the 10- to 20-microm tip region of long pollen tubes, only a few short F-actin fragments. Labeling with fluorescein isothiocyanate (FITC)-DNase I at first established the presence of a tip-focused gradient of intracellular G-actin concentration at the extreme apex of the tube, the concentration of G-actin being about twice as high in the 10- to 20-microm region of the tip as in other regions of the pollen tube. We also found that the distribution of G-actin was related negatively to that of the F-actin in pollen tubes of L. davidii. Caffeine treatment caused the G-actin tip-focused gradient to disappear, and F-actin to extend into the pollen tube tip. Based on these results, we speculate that the circular F-actin bundles may be the track for bidirectional cytoplasmic streaming in pollen tubes, and that in the pollen tube tip most of the F-actin is depolymerized into G-actin, leading to the absence of F-actin bundles in this region.
Li
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College of Biological Sciences, China Agricultural University, Beijing, PR China. liyan@mail.cau.edu.cn
Zee
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Liu
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Huang
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Yen
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D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage; D005455:Fluorescent Antibody Technique; D019085:Fluorescent Antibody Technique, Direct; D027762:Lilium / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D018613:Microscopy, Confocal; D011058:Pollen / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development; D012098:Reproduction
D000199:Actins; D002110:Caffeine; D016650:Fluorescein-5-isothiocyanate