The rpoS gene in Pseudomonas syringae is important in surviving exposure to the near-UV in sunlight.
The near-UV component of sunlight decreased culturability of the leaf epiphyte and plant pathogen Pseudomonas syringae. Exposure of the wild-type cells for 4 h to UV-A and UV-B in sunlight was ten fold more detrimental than exposure to sunlight with just UV-A. Sensitivity to UV-A especially increased in a mutant of P. syringae lacking the global regulatory sigma factor, RpoS. No RpoS-mutant cells were culturable after 4 h of exposure to near-UV sunlight. These findings suggest that both UV-A and UV-B wavelengths cause damage to the bacterial cell and that the RpoS protein regulates protective measures for the leaf-associated pseudomonad.
Miller
C D
CD
Department of Biology, Utah State University, Logan 84322-5305, USA.
Mortensen
W S
WS
Braga
G U
GU
Anderson
A J
AJ
D001426:Bacterial Proteins / Q000235:genetics*; D003470:Culture Media; D004307:Dose-Response Relationship, Radiation; D015964:Gene Expression Regulation, Bacterial; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development*; D012808:Sigma Factor / Q000235:genetics*; D013472:Sunlight; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D003470:Culture Media; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria