A modified spray reagent for the detection of amino acids on thin layer chromatography plates.
Identification of amino acids is extremely important for the evaluation of protein structure. Thin layer chromatography is an important tool for detecting amino acids by variety of spray reagents. Among these ninhydrin is the most popular due to its high sensitivity. However, ninhydrin produces the same purple/violet color with most amino acids. A spray reagent with high sensitivity for easy and rapid identification of amino acids on thin-layer plates has been introduced.
Laskar
S
S
Chemistry Department, The University of Burdwan, West Bengal, India.
Sinhababu
A
A
Hazra
K M
KM
D000596:Amino Acids / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003116:Color; D006146:Guanidines / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D009555:Ninhydrin / Q000737:chemistry
D000596:Amino Acids; D006146:Guanidines; D007202:Indicators and Reagents; D009555:Ninhydrin; C004711:dicyandiamido