Polyester microchannel chip for electrophoresis--incorporation of a blue LED as light source.
A blue-light-emitting diode was incorporated as a fluorescence-excitation light source into a polyester microchannel chip fabricated by in situ polymerization. Placing the light-emitting facet of the diode close to the microchannel obviated any need for an additional optical arrangement. Fluorescence from the sample was transmitted by an optical fiber incorporated into the microchip perpendicular to the LED. FITC labeled amino acids were separated in the presence of 5 mM SDS by using the microchip and were detected by LED-induced fluorescence.
Uchiyama
K
K
Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, Hachioji, Japan. uchiyama-katsumi@c.metro-u.ac.jp
Xu
W
W
Qiu
J
J
Hobo
T
T
D000596:Amino Acids / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005336:Fiber Optic Technology; D005453:Fluorescence; D008832:Microchemistry / Q000379:methods*; D055100:Optical Fibers; D010649:Phenylalanine / Q000032:analysis / Q000302:isolation & purification; D011091:Polyesters; D012680:Sensitivity and Specificity; D014633:Valine / Q000032:analysis / Q000302:isolation & purification
D000596:Amino Acids; D011091:Polyesters; D010649:Phenylalanine; D014633:Valine