Purification and characterization of a hemagglutinin isolated from the leaves of Chenopodium (Chenopodium amaranticolor).
A hemagglutinin was isolated and purified from the leaves of Chenopodium (Chenopodium amaranticolor) using ion-exchange chromatography and affinity chromatography on fetuin-agarose matrix. It agglutinated rabbit erythrocytes. The hemagglutinin had a native molecular mass of 58 kDa, as estimated by gel filtration and showed a single band of molecular mass of 33 kDa on SDS-PAGE. It showed hemagglutination activity over the pH range 3-12 and was found to be stable up to 70 degrees C. On isoelectrofocussing, the pI of this hemagglutinin was estimated to be 5.25. However, it was found to contain seven charge variants when isoelectrofocussing was performed in presence of 6M urea.
Suseelan
K N
KN
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India. nad@magnum.barc.ernet.in
Mitra
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R
D027462:Chenopodium / Q000737:chemistry*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006388:Hemagglutinins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008024:Ligands; D018515:Plant Leaves / Q000737:chemistry*; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors
D006388:Hemagglutinins; D008024:Ligands