Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas.
Cyclin D1 regulates G1-S progression. In many carcinomas it is overexpressed and it might even correlate with prognosis. However, the amplification of CCND1 contributes to the loss of cell cycle control only in a small fraction of malignant gliomas. Cyclin D1 can be immunohistochemically demonstrated by DCS-6 mAb. In astrocytic gliomas the fraction of tumor cells with positive nuclei is almost null in well differentiated tumors and increases with the increase of proliferation rate that occurs in anaplasia. The correct evaluation of this fraction is hindered by the positive staining of normal oligodendrocytes and microglia cells. The cyclin D1-positive staining of normal oligodendrocytes and microglia cells has been studied in a series of 20 oligodendrogliomas, five diffuse astrocytomas and five oligoastrocytomas and in 10 samples of normal cortex and white matter, using cyclin D1 DCS-6 mAb, Feulgen reaction and CR3.43 mAb for microglia cells. As well as microglial nuclei, the nuclei of normal oligodendrocytes of the cortex and white matter, including peri-neuronal satellites and pericapillary cells, were immunostained by DCS-6 mAb. In infiltrative areas of oligodendrogliomas, normal, cyclin D1-positive oligodendrocytes and cyclin D1-negative tumor cells coexisted. In anaplastic oligodendrogliomas, cycling tumor oligodendrocytes may regain the capacity to express cyclin D1, which is thus positive in some tumor cells. The occurrence of positive oligodendrocytes in the peripheral parts of tumors can be useful in distinguishing astrocytomas from oligoastrocytomas.
Bosone
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I
Department of Neuroscience, University of Turin, Italy.
Cavalla
P
P
Chiadò-Piat
L
L
Vito
N D
ND
Schiffer
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D
D000911:Antibodies, Monoclonal; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D004396:Coloring Agents; D019938:Cyclin D1 / Q000378:metabolism*; D003937:Diagnosis, Differential; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017628:Microglia / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D009837:Oligodendroglioma / Q000378:metabolism* / Q000473:pathology; D012394:Rosaniline Dyes
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; C007722:Feulgen stain; D005904:Glial Fibrillary Acidic Protein; D012394:Rosaniline Dyes; D019938:Cyclin D1