Recombinant Newcastle disease virus as a vaccine vector.
A complete cDNA clone of the Newcastle disease virus (NDV) vaccine strain Hitchner B1 was constructed, and infectious recombinant virus expressing an influenza virus hemagglutinin was generated by reverse genetics. The rescued virus induces a strong humoral antibody response against influenza virus and provides complete protection against a lethal dose of influenza virus challenge in mice, demonstrating the potential of recombinant NDV as a vaccine vector.
Nakaya
T
T
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Cros
J
J
Park
M S
MS
Nakaya
Y
Y
Zheng
H
H
Sagrera
A
A
Villar
E
E
García-Sastre
A
A
Palese
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D011995:Recombination, Genetic
D000914:Antibodies, Viral; D018076:DNA, Complementary