Sp1 is required for prolactin activation of the interferon regulatory factor-1 gene.
Transcription of the interferon regulatory factor-1 gene (IRF-1) is induced in a biphasic manner (G1 and G1/S phase) in Nb2 T cells in response to prolactin (PRL) stimulation. Signal transducer and activator of transcription 1 (Stat1) is required for PRL activation of the IRF-1 promoter. Mutation of a -200 bp Sp1 site in the IRF-1 promoter results in a loss of G1 but not G1/S IRF-1 transcriptional activity in response to PRL. These studies illustrate that the temporal transcription of the IRF-1 gene is mediated by not only Stat1 but also Sp1 in response to PRL stimulation.
Book McAlexander
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M
Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Yu-Lee
L Y
LY
D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D016193:G1 Phase; D050856:Interferon Regulatory Factor-1; D009154:Mutation; D010750:Phosphoproteins / Q000187:drug effects / Q000235:genetics*; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D011401:Promoter Regions, Genetic; D051381:Rats; D050794:STAT1 Transcription Factor; D015398:Signal Transduction; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015534:Trans-Activators / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; C494259:Irf1 protein, rat; D010750:Phosphoproteins; D050794:STAT1 Transcription Factor; D016329:Sp1 Transcription Factor; C494081:Stat1 protein, rat; D015534:Trans-Activators; D011388:Prolactin