title: [Identification of Amomum villosum, Amomum villosum var. xanthioides and Amomum longiligulare on ITS-1 sequence].
abstract: OBJECTIVE
  To identify Amomum villosum Lour. and some their adulterants on molecular biology.
  
  
  METHODS
  The DNA of Amomum villosum Lour. and some their adulterants were extracted, and amplified using ITS-1 primer. The amplificed DNA were purified and then sequenced by direct PCR sequencing method.
  
  
  RESULTS
  The ITS-sequence of all of the samples are 248 bp in size. But there are 7 bases in Amomum villosum Lour var. xanthioides (Wall.ex Bak) T.L. Wu et Senjen and 12 hases in Amomum longiligulare T.L. Wu. differing from Amomum villosum Lour.
  
  
  CONCLUSIONS
  The ITS-1 sequence can be used to identify effectively Amomum villosum Lour. and their adulterants.
authors.affiliation: Guangzhou University of TCM, Guangzhou 510405.
authors.forename: H
authors.initials: H
authors.lastname: Pan
authors.forename: F
authors.initials: F
authors.lastname: Huang
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: L
authors.initials: L
authors.lastname: Cao
authors.forename: R
authors.initials: R
authors.lastname: Liang
mesh_terms: D035801:Amomum / Q000235:genetics*; D001483:Base Sequence; D005638:Fruit / Q000235:genetics; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA