title: Eicosapentaenoic and docosahexaenoic acids production by and okara-utilizing potential of thraustochytrids.
abstract: Nine thraustochytrid strains isolated from subtropical mangroves were screened for their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production potential in a glucose yeast extract medium. Their ability to utilize okara (soymilk residue) for growth and EPA and DHA production was also evaluated. EPA yield was low in most strains, while DHA level was high on glucose yeast extract medium, producing 28.1-41.1% of total fatty acids, for all strains, with the exception of Ulkenia sp. KF13. The DHA yield of Schizochytrium mangrovei strains ranged from 747.7 to 2778.9 mg/l after 52 h of fermentation at 25 degrees C. All strains utilized okara as a substrate for growth, but DHA yield was lower when compared with fermentation in a glucose yeast extract medium.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, SAR, China.
authors.forename: K W
authors.initials: KW
authors.lastname: Fan
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: E B
authors.initials: EB
authors.lastname: Jones
authors.forename: L L
authors.initials: LL
authors.lastname: Vrijmoed
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis*; D056890:Eukaryota / Q000254:growth & development / Q000378:metabolism*; D008386:Marine Biology; D010940:Plant Proteins / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D045730:Soy Foods; D014197:Trees / Q000469:parasitology
chemical_list: D003470:Culture Media; D010940:Plant Proteins; D011134:Polysaccharides; C412882:okara, Glycine max; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid