Chemical composition and analgesic activity of Calophyllum brasiliense leaves.
This paper describes a phytochemical and pharmacological study with Calophyllum brasiliense leaves, a medicinal plant employed in folk medicine for the treatment of several ailments. Based on spectroscopic evidence, five phenolic compounds were identified as hyperin (hyperoside), amentoflavone, quercetin, gallic acid, and protocatechuic acid. The fractions and some phenolic compounds exhibited significant analgesic activity against the writhing test and in relation to the second phase (inflammatory pain) of the formalin test in mice, suggesting that this plant can be useful for the treatment of dolorous processes.
da Silva
K L
KL
Núcleo de Investigações Químico-Farmacêuticas (NIQFAR)/CCS, Universidade do Vale de Itajaí (UNIVALI), Rua Uruguai No. 458, C. Posta 88302-202, Itajaí SC, Brazil.
dos Santos
A R
AR
Mattos
P E
PE
Yunes
R A
RA
Delle-Monache
F
F
Cechinel-Filho
V
V
D015746:Abdominal Pain / Q000139:chemically induced / Q000188:drug therapy; D000085:Acetates / Q000737:chemistry; D019342:Acetic Acid / Q000633:toxicity; D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D044946:Biflavonoids; D001938:Brazil; D029759:Calophyllum / Q000737:chemistry*; D005591:Chemical Fractionation; D002855:Chromatography, Thin Layer; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000302:isolation & purification / Q000627:therapeutic use; D005528:Foot; D005557:Formaldehyde / Q000633:toxicity; D005707:Gallic Acid / Q000302:isolation & purification / Q000627:therapeutic use; D006586:Hexanes / Q000737:chemistry; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000627:therapeutic use; D007274:Injections, Intraperitoneal; D008297:Male; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D010636:Phenols / Q000302:isolation & purification / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000627:therapeutic use; D012997:Solvents / Q000737:chemistry
D000085:Acetates; D000700:Analgesics; D044946:Biflavonoids; D005419:Flavonoids; D006586:Hexanes; D062385:Hydroxybenzoates; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D005557:Formaldehyde; C009091:protocatechuic acid; D008752:Methylene Chloride; D005707:Gallic Acid; C007650:ethyl acetate; C021304:hyperoside; C011164:amentoflavone; D011794:Quercetin; D019342:Acetic Acid