[Analysis of the viral capsid gene of Alfalfa mosaic virus (CP A1MV) cloned to the vector for plant transformation pBI121].
The virus gene CP AlMV cloned to the vector pBI121 (Clontech) was analyzed using the method of restriction analysis and polymerase chain reaction. The restriction analysis with the use of ApaI enzyme has shown presence of the specific fragment with a part of CP gene about 2.5 kb. The same procedure with EcoRI and HindIII enzymes has shown difference in electrophoretic migration of the formed fragments. Polymerase chain reaction analysis has shown the existence of the specific amplification product of the CP gene 666 bp long. Such investigations have proved successful cloning and presence of the CP AlMV gene in vector pBI121 and could afford to make following transformation of the higher plants with attaching genetic tolerance to the virus from Bromoviridae family.
Mel'nychuk
M D
MD
National Agricultural University, 15 Heroiv Oborony St., Kyiv, 03041, Ukraine.
Spyrydonov
V G
VG
D017794:Alfalfa mosaic virus / Q000201:enzymology / Q000235:genetics*; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D005784:Gene Amplification; D005814:Genes, Viral; D005822:Genetic Vectors; D030821:Plants, Genetically Modified / Q000235:genetics; D015183:Restriction Mapping