title: Extracellular matrix molecules improve periodontal ligament cell adhesion to anorganic bone matrix.
abstract: Bone replacement graft (BRG) materials are used in periodontal therapy to encourage new bone formation. Extracellular matrix proteins may improve periodontal ligament fibroblast (PDLF) attachment to these materials. We demonstrate that PDLFs adhere well to the extracellular matrix (ECM) proteins fibronectin, vitronectin, laminin, and collagen types I and IV. PDLFs express numerous ECM-receptor integrin subunit transcripts (alpha1, alpha2, alpha3, alpha4, alpha5, alpha11, beta1, beta5, and beta8) at high levels, while others (alpha6, alpha9, alphaV, beta3, beta6, and beta7) are expressed at reduced levels. Despite the fact that PDLFs adhere well to fibronectin and collagen type IV bound to plastic, and express integrins that recognize these ECM proteins, they do not attach well to anorganic bovine bone matrix (ABM) coated with these same proteins. However, the addition of vitronectin, laminin, or collagen type I to these same ABMs substantially increased PDL cell attachment. Thus, selective use of ECM proteins may be clinically useful in promoting cell attachment to ABM and bone regrowth.
authors.affiliation: Department of Cell Biology and Anatomy, Louisiana State University Health Science Center, School of Dentistry, New Orleans 70119, USA. tlalli@lsuhsc.edu
authors.forename: T E
authors.initials: TE
authors.lastname: Lallier
authors.forename: R
authors.initials: R
authors.lastname: Yukna
authors.forename: R L
authors.initials: RL
authors.lastname: Moses
mesh_terms: D000327:Adsorption; D000818:Animals; D001857:Bone Matrix / Q000648:ultrastructure*; D018786:Bone Substitutes / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D003094:Collagen / Q000494:pharmacology; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010969:Plastics / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013223:Statistics as Topic; D013499:Surface Properties; D019096:Vitronectin / Q000494:pharmacology
chemical_list: D018786:Bone Substitutes; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D007797:Laminin; D010969:Plastics; D019096:Vitronectin; D003094:Collagen