[Dynamic change and expression of matrix metalloproteinase-2, -9 in alcoholic liver disease in rats].
OBJECTIVE
To evaluate the dynamic change and expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in alcoholic liver disease in rats.


METHODS
Alcoholic liver disease rat model was established by gavage. The expression of MMP-2 and MMP-9 was studied by immunohistochemistry, and the result was analyzed by picture quantitative analysis technique.


RESULTS
The expression of MMP-2 and MMP-9 was significantly higher in rats of model group than that of control group (P<0.05). The level of MMP-2 and MMP-9 increased gradually with the progress of alcoholic liver disease. Immunoreactive MMP-2 was expressed in endothelial cells of hepatic artery and portal vein, sinusoidal endothelial cells and sinusoidal cells. Immunoreactive MMP-9 was observed in sinusoidal cells and hepatic cells around the vein.


CONCLUSIONS
MMP-2 and MMP-9 increa with the progress of alcoholic liver disease. They might be involved in the development of alcoholic liver disease.
Lu
X
X
First Clinical College of China Medical University. Shenyang 110001, China.
Wang
B
B
Xie
Y
Y
Liu
C
C
Fu
B
B
D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008108:Liver Diseases, Alcoholic / Q000201:enzymology* / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9