Fine silver staining analysis of the nucleolar organizer regions during oogenesis in Penaeus kerathurus (Crustacea, Decapoda).
In previtellogenic oocytes, nucleoli showed segregated components, with the dense fibrillar component (DFC) appearing highly developed and presenting several fibrillar centers (FC). The granular component (GC) was less developed and formed a wide-spaced reticulum. Only the DFC appeared stained by silver, with higher intensities being found at its periphery. During early vitellogenesis, the nucleolar components were kept segregated but both the DFC and the GC enlarged, without evident changes being noticed in the silver staining pattern. In mid and late vitellogenesis, the nucleoli showed integrated components, with the DFC being intermeshed with the GC. Both nucleolar components were highly developed, no evident FC were noticed, and silver stained the DFC in a heterogeneous pattern. During cortical vesicle formation, the nuclear chromatin condensed and nucleoli appeared disintegrated, showing high levels of accelerated exportation of silver stained materials. Results suggest that the size of the DFC is kept high and the size of the GC kept low (low rDNA transcription levels and RNP exportation accelerated), in the segregated nucleoli of the previtellogenic oocyte, as the cell stores nuages but shows absence of rough endoplasmic reticulum and thus low protein svnthesis; that the size of the DFC and of the GC is increased in the segregated nucleoli of early vitellogenic oocytes (intermediate levels of rDNA transcription and of protein synthesis), which is in accordance with the appearance of the rough endoplasmic reticulum and of yolk vesicles formed with endogenous and exogenous sources; that during mid and late vitellogenesis the DFC and the GC appear highly developed and integrated (high levels of rDNA transcription and of protein synthesis) as the rough endoplasmic reticulum expands and the large yolk vesicles grow by endogenous synthesis; and that chromatin condense and nucleoli disintegrate (very low levels of rDNA transcription with accelerated RNP exportation) when cortical vesicles are formed.
Erkan
M
M
Faculty of Science, University of Istanbul, Turkey.
Sousa
M
M
Carvalho
E
E
Oliveira
E
E
Baldaia
L
L
D000818:Animals; D002466:Cell Nucleolus / Q000502:physiology / Q000648:ultrastructure; D003445:Crustacea / Q000502:physiology / Q000648:ultrastructure*; D005260:Female; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D016622:Silver Staining; D014818:Vitellogenesis / Q000502:physiology*