Mapping of rabbit chromosome 1 markers generated from a microsatellite-enriched chromosome-specific library.
A genomic DNA library was produced from flow-sorted rabbit chromosome 1 and enriched for fragments containing CA-repeats. Clones containing CA-repeats were identified and primers for amplification of the microsatellite were developed after sequencing the clone. The degree of polymorphism was tested in rabbits from different breeds. This approach identified 12 microsatellite markers which could be used for studying linkage relationships in the progeny of an F(2)-intercross: (AX/JUxIIIVO/JU) F(2), and two backcrosses: (OS/JxX/J)X/J and (WH/JxX/J)X/J. Seven of these markers were mapped on chromosome 1.
Korstanje
R
R
Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. rkorstan@jax.org
Gillissen
G F
GF
den Bieman
M G
MG
Versteeg
S A
SA
van Oost
B
B
Fox
R R
RR
van Lith
H A
HA
van Zutphen
L F
LF
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D003433:Crosses, Genetic; D018900:Dinucleotide Repeats; D005260:Female; D015698:Genomic Library; D008297:Male; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D011817:Rabbits / Q000235:genetics*