Effects of glucose and fructose on motility patterns of dog spermatozoa from fresh ejaculates.
This study was performed to gain insight about how fructose and glucose modulate dog spermatozoa motility in the absence of other motility-modulating factors. Incubation of dog spermatozoa from fresh ejaculates in a basal medium without sugars for 60 min at 37 degrees C induced a progressive decrease in the percentage of motile spermatozoa and in some mean motility parameters, such as mean velocity (VAP), linear coefficient (LIN) and dance (DNC), and an increase in the mean frequency of head displacement (BCF). This indicates a progressive loss of linearity and an increase in oscillatory movement. Addition of 10 mM fructose prevented these effects. Incubation in a basal medium with 10 mM glucose for 60 min at 37 degrees C provoked a fast and intense decrease of LIN and a slight increase of DNC, inducing a less linear and more oscillatory mean movement. Neither fructose nor glucose modified the percentage of motile spermatozoa. The response to both sugars was dose-dependent, with differences appearing at concentrations as low as 1 mM. An analysis of the spermatozoa subpopulation placed above the 95th percentile of the whole population and a factorial analysis of the data indicated that the changes in the mean values of the motility parameters were mainly due to a specific motile subpopulation that had a strong reaction to the two sugars. Our results indicate that fructose, at concentrations from 1 to 10 mM, induced a more linear and less oscillatory motility pattern than glucose. Moreover, from our results we suggest the presence of motile dog sperm subpopulations with an increased sensitivity to fructose and glucose.
Rigau
T
T
Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain.
Farré
M
M
Ballester
J
J
Mogas
T
T
Peña
A
A
Rodríguez-Gil
J E
JE
D000818:Animals; D004285:Dogs / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005632:Fructose / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D005947:Glucose / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008358:Mannose / Q000032:analysis; D012661:Semen / Q000737:chemistry; D013012:Sorbitol / Q000032:analysis; D013081:Sperm Motility / Q000187:drug effects*
D005632:Fructose; D013012:Sorbitol; D005947:Glucose; D008358:Mannose