[Experimental model of perinatal pulmonary circulation in lambs].
OBJECTIVE
Mechanisms that modulate fetal pulmonary circulation and transitional circulation at birth are incompletely understood. The aim of this experimental study was to describe an animal model in order to study the perinatal pulmonary circulation.


METHODS
Pregnant ewes were operated on between 126 and 128 days gestation (term = 145 days). A skin incision was performed to the fetal lambs in utero and catheters were placed into the ascending aorta and the superior vena cava after insertion in the axillary artery and vein. Then, catheters were inserted into the left pulmonary artery (LPA), main pulmonary artery, and left atrium via a thoracotomy. Moreover, an ultrasonic flow transducer, and an inflatable vascular occluder were placed around the LPA and around the ductus arteriosus. During 10 days, studies were performed in utero (possibly continued when fetal lambs were delivered by caesarean section). This chronically prepared animal may be used to perform hemodynamic studies according to different protocols (drugs injection to the fetus or to the ewes, ductus arteriosus compression, oxygen test). The main pulmonary artery, aortic, left atrial and amniotic pressures, heart rhythm, and flow signal were continuously recorded.


RESULTS
Eighteen pregnant ewes were operated on and nine only could be used for experimentation. This ovine model permitted several studies, particularly about effects of catecholamines on the pulmonary circulation, and about effects of ductus arteriosus compression on the pulmonary circulation.


CONCLUSIONS
Chronically instrumented fetal lambs are an excellent model in order to study the perinatal pulmonary circulation.
Jaillard
S
S
Clinique de chirurgie thoracique, hôpital Calmette, CHRU de Lille, 59037 Lille, France. sjaillard@chru-lille.fr
Houfflin-Debarge
V
V
Riou
Y
Y
Storme
L
L
Puech
F
F
Wurtz
A
A
D000818:Animals; D001011:Aorta / Q000502:physiology; D002404:Catheterization / Q000662:veterinary; D004195:Disease Models, Animal; D005260:Female; D006439:Hemodynamics; D006801:Humans; D007231:Infant, Newborn; D008168:Lung / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000196:embryology; D011247:Pregnancy; D011651:Pulmonary Artery / Q000502:physiology; D012039:Regional Blood Flow; D012756:Sheep / Q000502:physiology*; D016216:Ultrasonography, Prenatal / Q000379:methods / Q000662:veterinary; D014683:Vena Cava, Superior / Q000502:physiology