title: Role of the alpha(v)beta6 integrin in human oral squamous cell carcinoma growth in vivo and in vitro.
abstract: Expression of the alpha(v)beta6 integrin is strikingly upregulated in several types of carcinoma, including human oral squamous cell carcinoma (SCC). Employing a neutralizing monoclonal antibody to alpha(v)beta6, we investigated its role in cell adhesion, proliferation, migration, and in vivo growth of an invasive human SCC line, termed HSC-3. We found that alpha(v)beta6 is strictly required for HSC-3 cell growth in a three-dimensional collagen gel and also prominently contributes to cell migration in two different assay systems. In addition, the anti-alpha(v)beta6 antibody inhibited the invasive growth of HSC-3 cells transorally injected into nude mice. In the presence of the coinjected antibody, the average tumor size at 10 days was reduced by 59%. Histologically, antibody-treated tumors appeared less invasive than control tumors. Furthermore, intravenous application of a neutralizing antibody to the alpha(v) integrin subunit retarded HSC-3 tumor growth. These results point to a possible critical role of the alpha(v)beta6 integrin in controlling growth and invasion of human oral cancer cells.
authors.affiliation: Lung Biology Center, Department of Medicine, University of California, San Francisco, California 94143-0854, USA.
authors.forename: H
authors.initials: H
authors.lastname: Xue
authors.forename: A
authors.initials: A
authors.lastname: Atakilit
authors.forename: W
authors.initials: W
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: D M
authors.initials: DM
authors.lastname: Ramos
authors.forename: R
authors.initials: R
authors.lastname: Pytela
mesh_terms: D000818:Animals; D000906:Antibodies / Q000627:therapeutic use; D000951:Antigens, Neoplasm; D015197:Carcinogenicity Tests; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy; D017469:Receptors, Fibronectin / Q000502:physiology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D005353:Fibronectins; D016023:Integrins; D017469:Receptors, Fibronectin; C073878:integrin alphavbeta6