A new complex variant t(4;15;17) in acute promyelocytic leukemia: fluorescence in situ hybridization confirmation and literature review.
We report a 37-year-old male with acute promyelocytic leukemia (APL) harboring a complex translocation (4;15;17). Karyotypic analysis with R-banding of bone marrow cells revealed 46,XY,t(4;15;17)(q21;q22;q21). Fluorescence in situ hybridization analysis using painting probes for chromosomes 4, 15 and 17 and reverse transcriptase polymerase chain reaction analysis revealed three derivative chromosomes: der(4)t(4;15)(q21;q22), der(15)t(4;15;17)(q21;q22;q21), and del(17)(q21q22). This is the third report of such a translocation and the first confirmed by molecular methods. Considering reported similar cases, it is possible that 4q21 is a nonrandom breakpoint in APL with complex translocations and the gene involved in 4q21 should be investigated.
Liu
S
S
Laboratory of Genetics, Hematological Institute, Chinese Academy of Medical Sciences, 288 Nanjing Road, 300020, Tianjin, China.
Li
Q
Q
Pang
W
W
Bo
L
L
Qin
S
S
Liu
X
X
Teng
Q
Q
Qian
L
L
Wang
J
J
D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002894:Chromosomes, Human, Pair 4; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic