Identification of ribosome-associated viral and cellular basic proteins during the course of infection with herpes simplex virus type 1.
Herpes simplex virus type 1 (HSV-1) infection induces severe alterations of the translational apparatus, including the phosphorylation of a few ribosomal proteins, and the progressive association of several nonribosomal proteins to ribosomes. Therefore, we hypothesized that ribosomes themselves could contribute to the HSV-1-induced translational control of host and viral gene expression. As a prerequisite to test this hypothesis, we undertook the identification of the nonribosomal proteins associated to the ribosomes during the course of HSV-1 infection. After separation by two-dimensional polyacrylamide gel electrophoresis of basic proteins extracted from the ribosomal fraction, the identification of unknown protein spots was carried out by N-terminal sequencing and peptide mass determination by mass spectrometry. This allowed us to identify HSV-1 VP19C and VP26 that associated to ribosomes with different kinetics. Another nonribosomal protein turned out to be the poly(A)-binding protein 1 (PAB1P). Newly synthesized PAB1P continued to associate to ribosomes all along infection.
Greco
A
A
INSERM U369, Faculté de Médecine Lyon-R.T.H. Laennec, 7, Rue Guillaume Paradin, 69372 Lyon, France.
Bienvenut
W
W
Sanchez
J C
JC
Kindbeiter
K
K
Hochstrasser
D
D
Madjar
J J
JJ
Diaz
J J
JJ
D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006561:Herpes Simplex / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D006801:Humans; D007700:Kinetics; D014176:Protein Biosynthesis; D020543:Proteome; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D020543:Proteome; D012269:Ribosomal Proteins; D014764:Viral Proteins