title: Efficacy of periodic centrifugation of primates during 4-week head-down tilt.
abstract: Creation of artificial force of gravity (AFG) to counteract the negative consequences of microgravity in manned space missions of extended duration is one of the high-priority problems of space biology and medicine. However, there are a number of especial effects of AFG (namely, structural changes in muscles and bones, and some other system) which need implantation of electrodes and sensors and are possible only with animals. That is why it is of particular interest to make studies with monkeys whose reactions to changed gravity bear much resemblance with human. The purpose of the investigation was development of a protocol of periodic gravity loads as a counter-measure against the hypokinetic syndrome in Macaca mulatta. Two series of experiments were performed. In the series, animals were split into two groups of 6 species each who were motor restrained with the head end tilted downward at 5 degrees (HDT) for 28 days. Monkeys of group-2 were periodically subjected to centrifugation (HDT+G). During the first series of experiments rotation was conducted in the +Gz direction at g-loads from 1.2 to 1.6 units for 30-40 minutes 4-5 times a week. In the second series, g-load was equal to 1.2 units and the animals were rotated 30 min. 2-3 time a week. The criterion of Y-training protocol efficacy was a test +Gz run at 3 units for 30 s. during which functioning of the cardiovascular systems and its controls was evaluated. The test run was performed prior to and after HDT. Following HDT the animals of group HDT+G were more resistant to the test than their counterparts who had not been trained on the centrifuge. Data of the investigation imply that following HDT and HDT+G alike reduced the amount of total bodily fluids (by approximately 5%), the intracellular component (approximately 4%), and plasma volume (by 6-7%). Yet, there are radical differences between the groups in the levels of reduction in extracellular fluids (by 11% and 6.5%, respectively, P<0.05) and the interstitial component (by 11.5% and 6.5, respectively, P<0.05). Prophylactic centrifugation during HDT was also positive to the muscular blood flow in lower extremities.
authors.affiliation: RF SRC-Institute of Biomedical Problems, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Korolkov
authors.forename: I B
authors.initials: IB
authors.lastname: Kozlovskaya
authors.forename: A R
authors.initials: AR
authors.lastname: Kotovskaya
authors.forename: V P
authors.initials: VP
authors.lastname: Krotov
authors.forename: I F
authors.initials: IF
authors.lastname: Vil-Viliams
authors.forename: V I
authors.initials: VI
authors.lastname: Lobachik
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D002498:Centrifugation; D005110:Extracellular Space / Q000502:physiology; D018470:Gravity, Altered; D018475:Head-Down Tilt / Q000502:physiology*; D006339:Heart Rate; D018471:Hypergravity; D007424:Intracellular Fluid / Q000502:physiology; D008253:Macaca mulatta; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply; D010953:Plasma Volume / Q000502:physiology; D012039:Regional Blood Flow; D012149:Restraint, Physical; D018544:Weightlessness Countermeasures; D018474:Weightlessness Simulation