Internalization of ionotropic glutamate receptors in response to mGluR activation.
Activation of group 1 metabotropic glutamate receptors (mGluRs) stimulates dendritic protein synthesis and long-term synaptic depression (LTD), but it remains unclear how these effects are related. Here we provide evidence that a consequence of mGluR activation in the hippocampus is the rapid loss of both AMPA and NMDA receptors from synapses. Like mGluR-LTD, the stable expression of this change requires protein synthesis. These data suggest that expression of mGluR-LTD is at least partly postsynaptic, and that a functional consequence of dendritic protein synthesis is the regulation of glutamate receptor trafficking.
Snyder
E M
EM
Department of Neuroscience, Brown University, Providence, Rhode Island 02912, USA.
Philpot
B D
BD
Huber
K M
KM
Dong
X
X
Fallon
J R
JR
Bear
M F
MF
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003712:Dendrites / Q000378:metabolism; D004594:Electrophysiology; D004705:Endocytosis / Q000502:physiology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D018091:Receptors, AMPA / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012118:Resorcinols / Q000494:pharmacology; D013569:Synapses / Q000378:metabolism; D016704:Synapsins / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects; D014966:Xanthenes / Q000494:pharmacology
D000596:Amino Acids; D018691:Excitatory Amino Acid Antagonists; C114624:LY 341495; D011500:Protein Synthesis Inhibitors; D018091:Receptors, AMPA; D018094:Receptors, Metabotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D012118:Resorcinols; D016704:Synapsins; D014966:Xanthenes; D008734:Methoxyhydroxyphenylglycol; C079215:3,5-dihydroxyphenylglycine; D003513:Cycloheximide; D005998:Glycine; C010117:3,4-dihydroxyphenylglycol