Analysis of SIV-specific CTL in the rhesus macaque model of AIDS: the use of simian fibroblasts as an alternative source of target cells for chromium release assays.
The simian immunodeficiency virus (SIV) model of AIDS is widely used for the development of human immunodeficiency virus (HIV) vaccine strategies, particularly for the analysis of correlates of protective immunity. As it is not always possible to establish autologous B-lymphoblastoid cell lines (B-LCL) for use as targets in the analysis of cytotoxic T cell (CTL) activity, we have compared B-LCL with primary simian skin cells. Using a well-defined SIV gag-encoded CTL epitope restricted by Mamu A*01 major histocompatibility complex (MHC) class I, we have shown that peripheral blood mononuclear cells (PBMC) from vaccinated and infected macaques can kill MHC class I-matched skin fibroblasts presenting the cognate epitope but that skin fibroblasts are a less sensitive target than B-LCL for the detection of CTL.
Sharpe
S
S
Centre for Applied Microbiology and Research, Wiltshire SP4 0JG, Salisbury, UK. sally.sharpe@camr.org.uk
Beer
B
B
Hall
G
G
Dennis
M
M
Norley
S
S
Cranage
M
M
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002857:Chromium; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D015395:Histocompatibility Antigens Class I; D006801:Humans; D008253:Macaca mulatta; D009698:Nucleoproteins; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000956:Antigens, Viral; D015683:Gene Products, gag; D015395:Histocompatibility Antigens Class I; D009698:Nucleoproteins; D002857:Chromium; C090319:peptide 31D