Mutagenicity of a single 1-nitropyrene-DNA adduct N-(deoxyguanosin-8-yl)-1-aminopyrene in Escherichia coli located in a GGC sequence.
1-Nitropyrene, a common environmental pollutant, forms a major DNA adduct, N-(deoxyguanosin-8-yl)-1-amino- pyrene (dG(AP)). Mutational spectra of randomly introduced dG(AP) in Escherichia coli included different types of mutations, which depended on the base sequence surrounding the adduct. In earlier works we investigated the DNA sequence context effects of the adduct in repetitive CpG and non-repetitive CpGpC sequences. In the current work this adduct was incorporated into a non-repetitive GpGpC sequence in single-stranded M13mp7L2 DNA with the adduct located at either the 5' or 3' G. Potent genotoxicity of dG(AP) was evident from a significant reduction in the population of progeny phage following replication of these constructs in repair-competent E.coli cells. However, progeny derived from the 3'-G(AP) construct were much larger than those from the 5'-G(AP) construct. We suspect that a more facile translesion synthesis past the adduct at the 3' G relative to that at the 5' G, presumably due to a difference in conformation of dG(AP) in these two sites, might be responsible for this effect. With both adducted constructs, >95% of the progeny did not show any mutations at or near the adduct site, indicating highly efficient error-free translesion synthesis. However, a small population of mutants with one base deletions and base substitutions were detected. While the adduct induced -1 frameshifts (<1%) in each G site, base substitutions (1-2%), exhibiting predominantly G-->C transversions, were detected only when the adduct was located at the 5' G. A comparison of the data from this study with a prior study in the CpGpC sequence suggests that dG(AP) mutagenesis is highly sensitive to the local DNA sequence and that a 5'-pyrimidine base might be important for targeted base substitutions by this adduct in E.coli.
Bacolod
M D
MD
Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA.
Basu
A K
AK
D017104:Bacteriophage M13 / Q000235:genetics; D003596:Cytosine / Q000737:chemistry*; D018736:DNA Adducts / Q000138:chemical synthesis / Q000633:toxicity*; D004252:DNA Mutational Analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D016368:Frameshift Mutation / Q000235:genetics; D016679:Genome, Viral; D006147:Guanine / Q000737:chemistry*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics; D011721:Pyrenes / Q000633:toxicity*; D018911:Trinucleotide Repeats
D018736:DNA Adducts; D009153:Mutagens; D009841:Oligonucleotides; D011721:Pyrenes; D006147:Guanine; C045229:N-(deoxyguanosin-8-yl)-1-aminopyrene; D003596:Cytosine; D003849:Deoxyguanosine