Structure and localization of cyclosporin synthetase, the key enzyme of cyclosporin biosynthesis in Tolypocladium inflatum.
The electron microscopic image of native cyclosporin synthetase molecules showed large globular complexes of 25 nm in diameter, built up by smaller interconnected units. Compartmentation of cyclosporin synthetase and the functionally interconnected D-alanine racemase was revealed after sucrose density gradient centrifugation of subcellular fractions and immunoelectron microscopy. A considerable proportion of cyclosporin synthetase and D-alanine racemase was detected at the vacuolar membrane. The product cyclosporin was localized in the fungal vacuole.
Hoppert
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M
Institut für Mikrobiologie und Genetik, Grisebachstrasse 8, 37077 Göttingen, Germany. mhopper@gwdg.de
Gentzsch
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C
Schörgendorfer
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D000409:Alanine / Q000378:metabolism; D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D002499:Centrifugation, Density Gradient; D003524:Cyclosporins / Q000096:biosynthesis*; D015151:Immunoblotting; D007930:Leucine / Q000378:metabolism; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D009097:Multienzyme Complexes / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D010453:Peptide Synthases / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation
D000907:Antibodies, Bacterial; D003524:Cyclosporins; D009097:Multienzyme Complexes; C048525:cyclosporin A synthetase; D010453:Peptide Synthases; D007930:Leucine; D000409:Alanine