title: Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin.
abstract: The signal recognition particle (SRP) is a cytoplasmic RNA-protein complex that targets proteins to the rough endoplasmic reticulum. Although SRP functions in the cytoplasm, RNA microinjection and cDNA transfection experiments in animal cells, as well as genetic analyses in yeast, have indicated that SRP assembles in the nucleus. Nonetheless, the mechanisms responsible for nuclear-cytoplasmic transport of SRP RNA and SRP proteins are largely unknown. Here we show that the 19 kDa protein subunit of mammalian SRP, SRP19, was efficiently imported into the nucleus in vitro by two members of the importin beta superfamily of transport receptors, importin 8 and transportin; SRP19 was also imported less efficiently by several other members of the importin beta family. Although transportin is known to import a variety of proteins, SRP19 import is the first function assigned to importin 8. Furthermore, we show that a significant pool of endogenous SRP19 is located in the nucleus, as well as the nucleolus. Our results show that at least one mammalian SRP protein is specifically imported into the nucleus, by members of the importin beta family of transport receptors, and the findings add additional evidence for nuclear assembly of SRP.
authors.affiliation: Department of Biochemistry and Biophysics, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Dean
authors.forename: O
authors.initials: O
authors.lastname: von Ahsen
authors.forename: D
authors.initials: D
authors.lastname: Görlich
authors.forename: H M
authors.initials: HM
authors.lastname: Fried
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D028884:Karyopherins / Q000032:analysis / Q000378:metabolism*; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D018271:Signal Recognition Particle / Q000032:analysis / Q000378:metabolism*; D028961:beta Karyopherins / Q000032:analysis / Q000378:metabolism; D020931:ran GTP-Binding Protein / Q000032:analysis / Q000378:metabolism*
chemical_list: C107940:IPO5 protein, human; C466996:IPO7 protein, human; C107282:IPO8 protein, human; D028884:Karyopherins; D009687:Nuclear Proteins; D018160:Receptors, Cytoplasmic and Nuclear; C117074:SRP19 protein, human; D018271:Signal Recognition Particle; D028961:beta Karyopherins; D020931:ran GTP-Binding Protein