RNAse A treatment of Taq and Tth DNA polymerases eliminates primer/template-independent poly(dA-dT) synthesis.
Hanaki
K
K
Department of Medical Ecology, The Research Institute, International Medical Center of Japan, Tokyo. hanaki@ri.imcj.go.jp
Nishihara
T
T
Odawara
T
T
Nakajima
N
N
Yamamoto
K
K
Yoshikura
H
H
D001483:Base Sequence; D001709:Biotechnology; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry; D017931:DNA Primers / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D066298:In Vitro Techniques; D008970:Molecular Weight; D011067:Poly dA-dT / Q000096:biosynthesis*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology*; D019914:Taq Polymerase / Q000378:metabolism*
D017931:DNA Primers; D011067:Poly dA-dT; D004247:DNA; D019914:Taq Polymerase; C068508:Tth polymerase; D004259:DNA-Directed DNA Polymerase; D012259:Ribonuclease, Pancreatic