Induction of tumor clones in D. melanogaster wts/+ heterozygotes with chemical carcinogens.
Ten chemicals were assessed for blastomogenic activity in adult wts/+ heterozygotes of D. melanogaster. All of the strong mammalian carcinogens tested (benzo(a)pyrene (B(a)P), pyrene, aflatoxin B(1), 2-acetylaminofluorene (2-AAF) and cis-dichlorodihydroxydiamminoplatinum IV) were also shown to be strong Drosophila blastomogens. They induced several times more tumors than their counterparts that are less carcinogenic for mammals (4-acetylaminofluorene (4-AAF), aflatoxins B(2) and G(2)) and 4-(methylnitrosamino)-1-(-3-pyridine)-1-butanone (NNK). Benzo(e)pyrene (B(e)P) and pyrene demonstrated minor effects. Most tumors were localized on the wing and notum, which are the derivatives of the wing disc. Humeri derived from dorsal prothoracic disc and the abdominal tergites and sternites had the lowest number of tumors. The tumor frequency in the cross of the wild type females with wts(P2)/TM6B males was different from that in the reciprocal cross. The former type of cross exhibited consistently higher tumor frequency both in the experimental and control series.
Sidorov
R A
RA
Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow 115478, Russia.
Ugnivenko
E G
EG
Khovanova
E M
EM
Belitsky
G A
GA
D000348:Aflatoxins / Q000633:toxicity; D000577:Amides / Q000633:toxicity; D000818:Animals; D001681:Biological Assay / Q000379:methods; D015197:Carcinogenicity Tests / Q000379:methods*; D002273:Carcinogens / Q000633:toxicity*; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000633:toxicity; D002999:Clone Cells / Q000187:drug effects*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor / Q000187:drug effects; D006579:Heterozygote; D008297:Male; D009369:Neoplasms / Q000139:chemically induced; D009602:Nitrosamines / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000235:genetics
D000348:Aflatoxins; D000577:Amides; D002273:Carcinogens; D029721:Drosophila Proteins; D009602:Nitrosamines; D011084:Polycyclic Aromatic Hydrocarbons; D011494:Protein Kinases; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases; D002945:Cisplatin; C043804:diamminedichlorodihydroxyplatinum IV