Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography.
Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected in Korea. The stationary phase used was Zorbax 300 SB C18 and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and E in stems were higher than those in roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.
Kang
J S
JS
College of Pharmacy, Chungnam National University, Taejon 305-764, Korea.
Linh
P T
PT
Cai
X F
XF
Kim
H S
HS
Lee
J J
JJ
Kim
Y H
YH
D027481:Araliaceae / Q000737:chemistry*; D001208:Asia; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D017705:Lignans; D013058:Mass Spectrometry; D010666:Phenylpropionates / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D012997:Solvents; C421885:eleutheroside E; C028305:syringin