Degradation of 2-methylnaphthalene by Pseudomonas sp. strain NGK1.
Pseudomonas sp. strain NGK1, a soil bacterium isolated by naphthalene enrichment from biological waste effluent treatment, capable of utilizing 2-methylnaphthalene as sole source of carbon and energy. To deduce the pathway for biodegradation of 2-methylnaphthalene, metabolites were isolated from the spent medium and identified by thin-layer chromatography and high-performance liquid chromatography. The characterization of purified metabolites, oxygen uptake studies, and enzyme activities revealed that the strain degrades 2-methylnaphthalene through more than one pathway. The growth of the bacterium, utilization of 2-methylnaphthalene, and 4-methylsalicylate accumulation by Pseudomonas sp. strain NGK1 were studied at various incubation periods.
Sharanagouda
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Department of Biochemistry, Gulbarga University, Gulbarga-585 106, India.
Karegoudar
T B
TB
D001673:Biodegradation, Environmental; D003470:Culture Media; D007220:Industrial Waste; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009281:Naphthalenes / Q000378:metabolism*; D010101:Oxygen Consumption; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014865:Waste Disposal, Fluid
D003470:Culture Media; D007220:Industrial Waste; D009281:Naphthalenes; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C022619:salicylate 1-monooxygenase; C027384:2-methylnaphthalene