Determination of pethidine in rabbit plasma by capillary gas chromatography.
A simple and fast gas chromatographic method for the determination of pethidine hydrochloride in rabbit plasma is described and validated. This method is an improvement on the GC method which uses a glass column, and was described previously by Koska et al. [Anaesth. Analg. 60 (1981) 8]. The samples were analysed by a GC 5890 HP system using Rtx-5, fused-silica capillary column linked to a nitrogen-phosphorus ionization detector. Lidocaine hydrochloride was used as the internal standard. Calibration curves were linear within the range 5-200 ng/ml. The limit of quantification was about 5 ng using a 1-ml biological sample and no interference was observed in the blank plasma. The mean recovery of the drug from plasma samples was 95.71+/-2.82%. This procedure turned out to be sensitive and convenient enough for the use in pharmacokinetic studies of pethidine.
Szkutnik
D
D
Department of Clinical Pharmacy, Faculty of Pharmacy, Karol Marcinkowski University of Medical Sciences, 14 Sw. Marii Magdaleny Strasse, 61-861 Poznan, Poland. d.szkutnik@poczta.wp.pl
Dyderski
S
S
Majcher
K
K
D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D001682:Biological Availability; D002138:Calibration / Q000592:standards; D002849:Chromatography, Gas / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D007273:Injections, Intramuscular; D016014:Linear Models; D008614:Meperidine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D008614:Meperidine