Amyloid fibril formation in microwell plates for screening of inhibitors.
Fibril formation is the basis of amyloid production in a number of disease states, such as Alzheimer's disease, diabetes and immunocytic dyscrasias. Compounds that inhibit fibril formation could be directly relevant to the treatment of amyloid diseases, and may also provide a foundation for the development of interventions in other molecular condensation diseases ranging from sickle cell anemia to atherosclerosis. We developed an economical and convenient high-throughput method for screening compounds against fibril formation in microwell plates. Chalcones, flavonoids and biflavonoids were screened against fibril formation by a recombinant antibody variable domain (V1). Chalcones 6 and 14 were found to demonstrate inhibition at 0.1 microM in 79 microM of protein solution in both test tube and microwell plate assays. The concentration of protein in the microwell plate assay could be as low as 5 microM using ThT as a monitoring agent. Molecular modeling studies indicated that both compounds could be individually docked into a binding site at the monomer-monomer interface of the V(L) protein dimer. These studies suggested that these compounds could potentially stabilize the VL dimer and therefore reduce its tendency to form fibrils. These findings may provide the basis for a new therapeutic approach to prevent or treat amyloid diseases.
Lin
Y M
YM
MediChem Life Sciences, Inc., Woodridge, IL 60517, USA. ylin@medichem.com
Raffen
R
R
Zhou
Y
Y
Cassidy
C S
CS
Flavin
M T
MT
Stevens
F J
FJ
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D001665:Binding Sites; D002599:Chalcone / Q000378:metabolism / Q000494:pharmacology*; D019281:Dimerization; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000378:metabolism*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism
D016229:Amyloid beta-Peptides; D005419:Flavonoids; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; D002599:Chalcone