title: Photosystem II-based biosensors for the detection of pollutants.
abstract: Photosystem II (PSII) is the supramolecular pigment-protein complex in the chloroplast, which catalyses the light-induced transfer of electrons from water to plastoquinone (PQ) in a process that evolves oxygen. The PSII complex is also known to bind some groups of (photosynthetic) herbicides, heavy metals and other chemical substances that affect its activity. The objective of this study is to provide an overview of the systems available for the bioassay of pollutants using biosensors that are based on the photochemical activity of PSII. Some applications of the PSII-based biosensors including herbicide, heavy metal monitoring and the detection of radiation in space experiments are reported.
authors.affiliation: Institute of Plant Biochemistry and Ecophysiology, CNR, Via Salaria Km 29.3, 00016 Monterotondo Scalo, Rome, Italy. mariateresa.giardi@mlib.cnr.it
authors.forename: M T
authors.initials: MT
authors.lastname: Giardi
authors.forename: M
authors.initials: M
authors.lastname: Koblízek
authors.forename: J
authors.initials: J
authors.lastname: Masojídek
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D004785:Environmental Pollutants / Q000032:analysis*; D006540:Herbicides / Q000032:analysis; D019216:Metals, Heavy / Q000032:analysis; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D011834:Radiation Monitoring / Q000295:instrumentation / Q000379:methods
chemical_list: D004785:Environmental Pollutants; D006540:Herbicides; D019216:Metals, Heavy; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex