Interactions of surfactants with living cells. Induction of apoptosis by detergents containing a beta-lactam moiety.
The toxicity of new surfactants containing a beta-lactam ring has been established by studying their interaction with a hybridoma cell line. An hour of contact is sufficient to generate an apoptotic signal after two days of culture. Under the experimental conditions chosen for the experiments, surfactants have been divided into three categories: i) biocompatible and non-apogenic; ii) surfactants triggering an apoptotic signal without inducing cell necrosis; iii) surfactants triggering an apoptotic signal at low concentrations and destroying the cells by necrosis at higher concentrations. The necrosis inducing surfactants also had haemolytic properties. These properties were related to the values of the hydrophilic-lipophilic balance of the molecules.
Perani
A
A
Bâtiment INSERM, EA 2402 Université Henri Poincaré-Nancy I, Vandoeuvre-lès-Nancy, France
Gérardin
C
C
Stacey
G
G
Infante
M R
MR
Vinardell
P
P
Rodehüser
L
L
Selve
C
C
Maugras
M
M
D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D003902:Detergents / Q000737:chemistry / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D013501:Surface-Active Agents / Q000737:chemistry / Q000633:toxicity*; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured; D047090:beta-Lactams / Q000032:analysis / Q000737:chemistry*
D003902:Detergents; D013501:Surface-Active Agents; D047090:beta-Lactams