Cloning and characterization of a chicken platelet-derived growth factor B-chain cDNA.
Avian thrombocytes are nucleated blood cells homologous in function to mammalian platelets. In the present study, we obtained a cDNA from chicken thrombocyte polyadenylated RNA [Poly(A)+RNA], which coded for the chicken PDGF-B chain. The sequence was 1083-bp long and had an open reading frame (ORF) of 753-bp. At the amino acid level, the predicted mature protein showed 69% homology with the processed coding region of human PDGF-B. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that PDGF-B mRNA was expressed at high levels in thrombocytes and in the lung. The expression of PDGF-B chain mRNA in thrombocytes reached its maximum level 12h following type 1 collagen treatment. These results suggest that chicken PDGF-B chain may play an important role in the vascular system and in healing wounded tissue.
Horiuchi
H
H
Department of Immunobiology, Faculty of Applied Biological Science, Hiroshima University, Kagamiyama, 739-8528, Higashi-Hiroshima, Japan. hhori10@hiroshima-u.ac.jp
Inoue
T
T
Furusawa
S
S
Matsuda
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001773:Blood Cells / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D008168:Lung / Q000276:immunology; D008969:Molecular Sequence Data; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014945:Wound Healing
D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger