Expression of CC chemokines and their receptors in the eye in autoimmune anterior uveitis associated with EAE.
OBJECTIVE
To determine the pattern of expression of CC chemokines and their receptors in the eyes of Lewis rats and to establish their role in autoimmune anterior uveitis (AU) associated with experimental autoimmune encephalomyelitis (EAE).


METHODS
EAE/AU was induced in Lewis rats with myelin basic protein in complete Freund's adjuvant (CFA). The rats were scored for the development of clinical EAE and AU. The expression of CCL5/regulated on activation normal T-cell expressed and secreted (RANTES), CCL2/monocyte chemotactic protein (MCP)-1, CCL3/macrophage inflammatory protein (MIP)-1alpha, and CCL4/MIP-1beta and their receptors was examined at the preclinical stage, onset, peak, and recovery by RT-PCR and ELISA. EAE/AU rats were treated with neutralizing polyclonal antibodies against CCL3/MIP-1alpha, CCL4/MIP-1beta, CCL2/MCP-1, and CCL5/RANTES and tested for the suppression of onset of clinical AU and EAE. The control group received normal rabbit IgG at the same dose.


RESULTS
The gene expression of those chemokines was upregulated concurrently with symptom onset of EAE/AU and correlated with the intensity of inflammatory changes in the eye and central nervous system (CNS). The highest expression of CCL4/RANTES, CCL2/MCP-1, and CCL3/MIP-1alpha in the eye was detected at onset of clinical uveitis, whereas CCL4/MIP-1beta was elevated at the peak of AU. The expression of chemokine receptors associated with T-helper (Th)1-type response, CCR1 and CCR5, correlated with their appropriate ligands and was the highest at the peak of AU, whereas CCR2, the receptor for CCL2/MCP-1, was present before the onset of the disease. Treatment of anti-MIP-1beta and anti-MCP-1 significantly delayed the onset and shortened the duration of AU and EAE. Anti-MIP-1alpha treatment had no effect on clinical EAE but inhibited the clinical signs of AU. Although CCL5/RANTES expression was observed during the entire course of the disease, anti-RANTES treatment had no effect on clinical disease progression.


CONCLUSIONS
The data suggest that CCL2/MCP-1, CCL3/MIP-1alpha, and CCL4/MIP-beta contribute to the recruitment of inflammatory cells into the eye and CNS and to disease activity.
Adamus
G
G
Neurologic Sciences Institute, Oregon Health and Science University, Portland, USA. adamusg@ohsu.edu
Manczak
M
M
Machnicki
M
M
D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D019742:Chemokines, CC / Q000235:genetics* / Q000378:metabolism; D002924:Ciliary Body / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D007498:Iris / Q000378:metabolism; D004676:Myelin Basic Protein; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000378:metabolism; D015854:Up-Regulation; D014606:Uveitis, Anterior / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology
D019742:Chemokines, CC; D017931:DNA Primers; D004676:Myelin Basic Protein; D012333:RNA, Messenger; D019707:Receptors, Chemokine