title: The(13)C-octanoic acid breath test for detection of effects of meal composition on the rate of solid-phase gastric emptying in ponies.
abstract: The aim of this study was to apply the(13)C-octanoic acid breath test for detection of alterations in the rate of solid-phase gastric emptying, induced by changes in test meal composition, in ponies. After a 14 hour fast the ponies (n = 4) ingested a test meal with 0, 35 or 70 ml soya oil, and labelled with 250 mg(13)C-octanoic acid. Each pony was given each of the three test meals on three separate occasions, in a randomised order. Exhaled breath samples were collected for 12 hours after ingestion of the test meal. Breath samples were analysed by continuous flow isotope ratio mass spectrometry. Three indices of breath(13)C-enrichment were computed, half-dose recovery time (t 1/2), gastric emptying coefficient (GEC) and time to peak breath(13)C-excretion t(max). The(13)C-octanoic acid breath test was a reliable means of assessing the significantly decreased rate of gastric emptying in the pony, associated with addition of soya oil to the test meal.
authors.affiliation: Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden, Glasgow, G61 1QH. 9707285 w@student.gla.ac.uk
authors.forename: C A
authors.initials: CA
authors.lastname: Wyse
authors.forename: D M
authors.initials: DM
authors.lastname: Murphy
authors.forename: T
authors.initials: T
authors.lastname: Preston
authors.forename: D G
authors.initials: DG
authors.lastname: Sutton
authors.forename: D J
authors.initials: DJ
authors.lastname: Morrison
authors.forename: R M
authors.initials: RM
authors.lastname: Christley
authors.forename: S
authors.initials: S
authors.lastname: Love
mesh_terms: D000821:Animal Feed; D000818:Animals; D001944:Breath Tests; D002210:Caprylates / Q000008:administration & dosage / Q000032:analysis*; D002247:Carbon Isotopes; D018592:Cross-Over Studies; D005746:Gastric Emptying / Q000502:physiology*; D006736:Horses / Q000378:metabolism / Q000502:physiology*; D011897:Random Allocation
chemical_list: D002210:Caprylates; D002247:Carbon Isotopes; C031492:octanoic acid