title: Microbial inhibitors for U.S. EPA drinking water methods for the determination of organic compounds.
abstract: Preservation of chemical analytes in drinking water samples is necessary to obtain accurate information concerning contaminant occurrence. Sample preservation to prevent biodegradation is important for most samples and analytes. With the unique demands of environmental methods, it is not always possible to kill all microorganisms without having undesirable effects. To find a suitable preservative, the sample, analysis, and preservation needs should be considered. During method development of U.S. Environmental Protection Agency (EPA) Methods 526 (for unstable semivolatile compounds) and 532 (for phenylurea pesticides), a number of studies were conducted to identify compatible microbial inhibitors. Copper sulfate was successfully used in Method 532 and is an excellent first-choice antimicrobial agent for many applications. Copper sulfate can catalyze hydrolysis reactions for some pesticides such as those analyzed in Method 526. Under these conditions, a nonmetal compound of antimicrobial activity must be considered. During the development of Method 526, a survey of alternate organic based antimicrobial compounds found that diazolidinyl urea worked well in the method. Several other candidate microbial inhibitors were identified that could have application to other environmental methods. A general approach to selecting antimicrobial compounds in future environmental methods in water matrixes is discussed.
authors.affiliation: IT Corporation, Cincinnati, Ohio 45268, USA. winslow.stephen@epa.gov
authors.forename: S D
authors.initials: SD
authors.lastname: Winslow
authors.forename: B V
authors.initials: BV
authors.lastname: Pepich
authors.forename: M V
authors.initials: MV
authors.lastname: Bassett
authors.forename: S C
authors.initials: SC
authors.lastname: Wendelken
authors.forename: D J
authors.initials: DJ
authors.lastname: Munch
authors.forename: J L
authors.initials: JL
authors.lastname: Sinclair
mesh_terms: D000891:Anti-Infective Agents, Local / Q000737:chemistry; D000931:Antidotes / Q000737:chemistry; D019327:Copper Sulfate / Q000737:chemistry; D004784:Environmental Monitoring; D009930:Organic Chemicals / Q000032:analysis*; D013048:Specimen Handling; D014481:United States; D014484:United States Environmental Protection Agency; D014508:Urea / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply
chemical_list: D000891:Anti-Infective Agents, Local; D000931:Antidotes; D009930:Organic Chemicals; D014874:Water Pollutants, Chemical; D014508:Urea; C044982:diazolidinylurea; D019327:Copper Sulfate