[Some proposals for anaerobic primary culture from the supply side].
Many researchers paid attention to Bacteroides fragilis group chiefly for the past this 20 years. Most of B. fragilis groups encountered in clinical specimens possesses beta-lac- tamase. However recently, many researchers report that beta-lactamase-producing anaerobic Gram-negative bacilli except for B. fragilis groups are often encountered in clinical specimens. These strains belonging to Prevotella spp. or Fusobacterium spp. These organisms are often coexist with streptococci such as "S. milleri" group in specimens. Because these facts are very important in chemotherapy, such as beta-lactamase producers must be detected securely. For such a specimen, selective media that phenylethyl alcohol or gentamicin was added is not suitable. On the other hand, paromomycin vancomycin Brucella HK agar is suitable selective medium for not only these organisms but also for B. fragilis. Therefore, paromomycin vancomycin Brucella HK agar is recommended as the first choice of selective agar to use together with non-selective agar at present.
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Department of Research & Development, Kyokuto Pharmaceutical Ind. Co., Ltd., 3333-26 Aza-Asayama, Kamitezuna, Takahagi-shi, Ibaraki 318-0004, Japan.
D000362:Agar; D001431:Bacteriological Techniques; D001441:Bacteroides fragilis / Q000201:enzymology / Q000302:isolation & purification; D003470:Culture Media; D006801:Humans; D010303:Paromomycin; D014640:Vancomycin; D001618:beta-Lactamases / Q000096:biosynthesis
D003470:Culture Media; D010303:Paromomycin; D014640:Vancomycin; D000362:Agar; D001618:beta-Lactamases