Application of Cre-loxP system to the urinary tract and cancer gene therapy.
The bacteriophage P1-derived Cre-loxP system is a powerful and versatile tool for in vivo DNA recombination. It is widely used in gene targeting research. The combination of the Cre-loxP system and a specific promoter enables conditional "knockout" of a target gene in a particular tissue or cell type. It has also made it possible to delete genes in adult animals that are essential for embryogenesis. This system is also used to enhance tissue- or tumor-specific promoter activities that are useful for gene therapy of certain types of cancer. It is expected that this simple and effective system will be further utilized in various research applications.
Yoshimura
I
I
Department of Urology, National Defense Medical College, Tokorozawa, Saitama, Japan. ichiro-y@me.ndmc.ac.jp
Suzuki
S
S
Hayakawa
M
M
D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting; D015316:Genetic Therapy; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D051379:Mice; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014553:Urinary Tract Physiological Phenomena; D014571:Urologic Neoplasms / Q000235:genetics / Q000628:therapy*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases