Assembly of microarrays for genome-wide measurement of DNA copy number.
We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05-0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.
Snijders
A M
AM
Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143, USA.
Nowak
N
N
Segraves
R
R
Blackwood
S
S
Brown
N
N
Conroy
J
J
Hamilton
G
G
Hindle
A K
AK
Huey
B
B
Kimura
K
K
Law
S
S
Myambo
K
K
Palmer
J
J
Ylstra
B
B
Yue
J P
JP
Gray
J W
JW
Jain
A N
AN
Pinkel
D
D
Albertson
D G
DG
D000782:Aneuploidy; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D005260:Female; D018628:Gene Dosage; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D011123:Polyploidy; D014407:Tumor Cells, Cultured; D014960:X Chromosome / Q000235:genetics