title: Traumatic brain injury-induced changes in gene expression and functional activity of mitochondrial cytochrome C oxidase.
abstract: Traumatic brain injury (TBI) is documented to have detrimental effects on CNS metabolism, including alterations in glucose utilization and the depression of mitochondrial oxidative phosphorylation. Studies on mitochondrial metabolism have also provided evidence for reduced activity of the cytochrome oxidase complex of the electron transport chain (complex IV) after TBI and an immediate (lhr) reduction in mitochondrial state 3 respiratory rate, which can persist for up to 14 days postinjury. Using differential display methods to screen for differences in gene expression, we have found that cytochrome c oxidase II (COII), a mitochondrial encoded subunit of complex IV, is upregulated following TBI. Since COII carries a binding site for cytochrome c in the respiratory chain, and since it is required for the passage of chain electrons to molecular oxygen, driving the production of ATP, we hypothesized that metabolic dysfunction resulting from TBI alters COII gene expression directly, perhaps influencing the synaptic plasticity that occurs during postinjury recovery processes. To test this hypothesis, we documented COII mRNA expression and complex IV (cytochrome c oxidase) functional activity at 7 days postinjury, focusing on the long-term postinjury period most closely associated with synaptic reorganization. Both central fluid percussion TBI and combined TBI and bilateral entorhinal cortical lesion were examined. At 7 days survival, differential display, RT-PCR, and Northern blot analysis of hippocampal RNA from both TBI and combined insult models showed a significant induction of COII mRNA. This long-term elevation in COII gene expression was supported by increases in COII immunobinding. By contrast, cytochrome oxidase histochemical activity within tissue sections from injured brains suggested a reduction of complex IV activity within the TBI cases, but not within animals subjected to the combined insult. These differences in cytochrome c oxidase activity were supported by in vitro assay of complex IV using cerebral cortical and hippocampal tissues. Our present results support the hypothesis that COII is selectively vulnerable to TBI and that COII differences may indicate the degree of metabolic dysfunction induced by different pathologies. Taken together, such data will better define the role of metabolic function in long-term recovery after TBI.
authors.affiliation: The Department of Anatomy Virginia Commonwealth University, Richmond 23298-0709, USA.
authors.forename: L K
authors.initials: LK
authors.lastname: Harris
authors.forename: R T
authors.initials: RT
authors.lastname: Black
authors.forename: K M
authors.initials: KM
authors.lastname: Golden
authors.forename: T M
authors.initials: TM
authors.lastname: Reeves
authors.forename: J T
authors.initials: JT
authors.lastname: Povlishock
authors.forename: L L
authors.initials: LL
authors.lastname: Phillips
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology; D018728:Entorhinal Cortex / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007150:Immunohistochemistry; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D003576:Electron Transport Complex IV