Purification and partial amino acid sequencing of a mycorrhiza-related chitinase isoform from Glomus mosseae-inoculated roots of Pisum sativum L.
Colonization of Pisum sativum L. cv. Frisson roots with the arbuscular mycorrhizal fungus Glomus mosseae leads to the induction of four acidic symbiosis-related chitinase (SR-chi) isoforms (EC 3.2.1.14). These isoforms were characterized as 30-kDa proteins with isoelectric points ranging between 5.2 and 5.85. One of these SR-chis was purified by affinity and anion-exchange chromatographies, and isoelectric focusing electrophoresis. The sequences of four internal peptides were obtained. They showed high homology to a class-I chitinase isoform from pea shoots. Parts of the conserved regions of class-I chitinases were found in this SR-chi. This result strongly supports the argument that this SR-chi isoform is of plant origin. The functional role of the SR-chis in arbuscular mycorrhizal symbiosis is discussed.
Slezack
S
S
UMR BBCE-IPM INRA, Université de Bourgogne, CMSE, Institut National de la Recherche Agronomique, Dijon, France.
Negrel
J
J
Bestel-Corre
G
G
Dumas-Gaudot
E
E
Gianinazzi
S
S
D000595:Amino Acid Sequence; D002688:Chitinases / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000254:growth & development*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology* / Q000382:microbiology; D018517:Plant Roots / Q000201:enzymology* / Q000382:microbiology; D020539:Sequence Analysis, Protein; D013559:Symbiosis
D007527:Isoenzymes; D002688:Chitinases