Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate).
Large scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)] by Aeromonas hydrophila 4AK4 was examined in a 20,000 l fermentor. Cells were first grown using glucose as a carbon source, and polyhydroxyalkanoate (PHA) biosynthesis was triggered by the addition of lauric acid under conditions of limited nitrogen or phosphorus. When cells first grown in a medium containing 50 g glucose l(-1) were further cultivated after the addition of 50 g lauric acid l(-1) under phosphorus limitation, a final cell concentration, PHA concentration and PHA content of 50 g l(-1), 25 g l(-1), and 50 wt%, respectively, were obtained in 46 h, equivalent to PHA productivity of 0.54 g l(-1)t h(-1). The copolymer produced was found to be a random copolymer, and the 3HHx fraction was 11 mol%.
Chen
G Q
GQ
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Zhang
G
G
Park
S J
SJ
Lee
S Y
SY
D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D016980:Aeromonas hydrophila / Q000378:metabolism*; D002208:Caproates / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007850:Lauric Acids / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D020155:3-Hydroxybutyric Acid; D002208:Caproates; D005947:Glucose; D007850:Lauric Acids; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates; C030358:lauric acid