Comparative porcine gene mapping relative to human chromosomes 9, 10, 20 and 22.
Comparative anchor tagged sequence (CATS) consensus primers from loci mapped to human chromosomes 9, 10, 20, and 22 have been used to amplify homologous loci in pigs. Of 53, CATS primers tested in pigs, only 23 yielded products homologous to the human locus (42% success). Ten loci were physically mapped (43% success rate for verified products, but only 19% for primers tested). Due to lack of polymorphism, linkage mapping was possible only for AMBP. Map locations were consistent with human/pig ZOO-FISH, except for ADRA1A, whose position is still equivocal in humans. These CATS primers have made very limited contributions to pig/human comparative gene mapping because of low efficiency of amplification of orthologous porcine product, frequent amplification from rodent template in a somatic hybrid panel and low level of polymorphism.
Lee
J H
JH
Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney, NSW 2006, Australia.
Zhang
W
W
Moran
C
C
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D002879:Chromosomes, Human, Pair 10; D002890:Chromosomes, Human, Pair 20; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D008040:Genetic Linkage; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine / Q000235:genetics*; D026801:Synteny
D017931:DNA Primers