Identification of protein-bound carbohydrates by mass spectrometry.
This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix-assisted lased desorption/ionization, for the identification of protein-bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. Fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.
Harvey
D J
DJ
Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OXI 3QU, UK. dh@glycob.ox.ac.uk
D000581:Amidohydrolases; D000818:Animals; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D006023:Glycoproteins / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D020543:Proteome; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002241:Carbohydrates; D006023:Glycoproteins; D011134:Polysaccharides; D020543:Proteome; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase