[Effect of parenteral administration of melatonin on the biochemical composition of the rat granulation tissue].
The influence of long term parenteral melatonin administration on the biochemical composition of granulation tissue of surgical wounds in rats during healing was investigated. Physiological solution and solutions containing various concentrations of melatonin were subcutaneously injected to the animals within 3 weeks. Control and injected animals were wounded. Samples of gramilation tissue were investigated on the 5-th and the 8-th day of healing. The contents of oxyprolin, uronic acids, hexosamines, total lipids and their fractions, fractional composition of glycosaminoglicans and proteins composition of salt extracts were determined in the se samples. Repeated injections during three weeks caused the changes in biochemical composition of researched samples which were characteristic for stressful reaction of connective tissue. The specific changes are most expressed at long term introduction of a physiological solution to animals. The introduction of melatonin during similar period cansed protective effect, partially defending biochemical composition of granulation tissue from changes, which were induced by stressful situation.
Volodina
T V
TV
Research Centre of Biomedical Technology, 123056 Moscow, Krasin str., 2.
Ol'shevskiĭ
E G
EG
Abramov
Iu V
IuV
Guseva
V V
VV
Vinogradova
E V
EV
Gribanov
G A
GA
Kozel'tsev
V L
VL
Bykov
V A
VA
D000818:Animals; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D006097:Granulation Tissue / Q000737:chemistry / Q000187:drug effects*; D006595:Hexosamines / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis; D007263:Infusions, Parenteral; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014574:Uronic Acids / Q000032:analysis; D014945:Wound Healing / Q000187:drug effects*
D006595:Hexosamines; D014574:Uronic Acids; D008550:Melatonin; D006909:Hydroxyproline