Adociasulfates 1-6, Inhibitors of Kinesin Motor Proteins from the Sponge Haliclona (aka Adocia) sp.
Adociasulfates 1-6 (1-6) were isolated from an extract of the Palauan sponge Haliclona (aka Adocia) sp. that inhibited the transport of stabilized microtubules by the motor protein kinesin, which was immobilized on a microscope slide. The structures of adociasulfates 1-6, the relative stereochemistry of adociasulfates 1, 2, 5, and 6, and the relative stereochemistry of subunits of adociasulfates 3 and 4 were determined by interpretation of spectroscopic data. In a quantitative assay that measures ATP hydrolysis by kinesin, adociasulfates 2 and 6 were the most active.
Blackburn
Christine L.
CL
Scripps Institution of Oceanography, University of California at San Diego, La Jolla, California 92093-0212, and Department of Pharmacology, Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093-0683.
Hopmann
Cordula
C
Sakowicz
Roman
R
Berdelis
Michael S.
MS
Goldstein
Lawrence S. B.
LS
Faulkner
D. John
DJ