title: Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage.
abstract: Immunohistochemical investigation of the post-translational processing of chromogranin A (CgA) to generate WE-14 in the sympathoadrenal cell lineage of the developing porcine fetus (F) detected intense CgA and weak WE-14 immunoreactivity in migrating neuroblast cells of the diffuse sympathetic ganglia adjacent to the dorsal aorta and projecting toward the cortical mass at F24-27. F37-42; WE-14 immunoreactivity was detected in chromaffinoblasts at the periphery of the developing cortex and at F54-56 days gestation WE-14 immunoreactivity was detected in a large population of central medullary cells. From F74 to F76 days and thereafter the number of cells exhibiting intense WE-14 immunostaining decreased, and the majority of chromaffin cells exhibited uniform weak WE-14 immunostaining. At postnatal day 1 (P1) intense WE-14 immunoreactivity was primarily confined to clusters of chromaffin cells with weak immunostaining in the general population. The transitory neuroblasts, chromaffinoblasts, and maturing chromaffin cell population exhibited uniform intense CgA immunostaining through gestation and after birth. Additional observations detected intense CgA and WE-14 immunostaining in extrachromaffin tissue at P1 and in neuronal-like cells in vessels of the aortic arch at F37. This study has demonstrated that CgA is post-translationally processed to generate WE-14 during early fetal development in the migrating progenitor cells of the porcine sympathoadrenal lineage.
authors.affiliation: Department of Medicine, The Queen's University of Belfast, Grosvenor Road, Belfast BT12 6BJ, UK.
authors.forename: S C
authors.initials: SC
authors.lastname: Barkatullah
authors.forename: K M
authors.initials: KM
authors.lastname: Pogue
authors.forename: J
authors.initials: J
authors.lastname: Depreitere
authors.forename: A
authors.initials: A
authors.lastname: Boutajangout
authors.forename: F
authors.initials: F
authors.lastname: Liang
authors.forename: W
authors.initials: W
authors.lastname: DePotter
authors.forename: W J
authors.initials: WJ
authors.lastname: Curry
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry* / Q000166:cytology / Q000196:embryology; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001011:Aorta / Q000737:chemistry / Q000196:embryology; D019070:Cell Lineage; D019439:Chromaffin Cells / Q000737:chemistry; D053379:Chromogranin A; D002864:Chromogranins / Q000032:analysis; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis*; D010234:Paraganglia, Nonchromaffin / Q000737:chemistry / Q000196:embryology; D013552:Swine; D013997:Time Factors
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D009363:Neoplasm Proteins; C074381:chromogranin A-derived peptide, WE-14