New supports for enzyme immobilization based on copolymers of vinylene carbonate and acrylamide.
In this study, a series of beadlike and hydrophilic supports containing reactive cyclic carbonate groups for enzyme immobilization were prepared via reverse-phase suspension copolymerization of the aqueous solutions of vinylene carbonate (VCA), acrylamide (AA), and N,N'-methylene bisacrylamide in paraffin oil. The supports were used as a matrix for immobilization of trypsin and showed a considerable capacity to couple with trypsin and reasonable retention of activity for the immobilized trypsin, depending on the immobilization conditions, such as the content of VCA structural units, reaction time, and pH of the medium.
Ding
L
L
Department of Chemistry, Nankai University, Tianjin, PR China. luding@chemistry.ohio-state.edu
Jiang
Y
Y
Huang
L
L
Li
Y
Y
Huang
J
J
D020106:Acrylamide / Q000737:chemistry*; D000178:Acrylamides / Q000737:chemistry; D004149:Dioxoles / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism
D000178:Acrylamides; D004149:Dioxoles; D004800:Enzymes, Immobilized; D020106:Acrylamide; C031134:vinylene carbonate; D014357:Trypsin; C021221:N,N'-methylenebisacrylamide