title: The infiltration kinetics of simian immunodeficiency virus-specific T cells drawn to sites of high antigenic stimulation determines local in vivo viral escape.
abstract: Despite vigorous cell-mediated immune responses to human and simian immunodeficiency viruses (HIV/SIV) the immune system is unable to clear latently infected resting T cells. These infected cells are reactivated by antigenic stimulation, leading to viral replication. By using the SIV/macaque model of HIV pathogenesis, the dynamics of T cell infiltration into delayed type hypersensitivity sites specific for the purified protein derivative of bacillus Calmette-Guérin have been studied. Early viral mRNA synthesis coincided with the infiltration of antigen-specific T cells. When the infiltration of anti-SIV-specific T cells was rapid compared with the kinetics of viral assembly, the sites were sterilized before the transition to late viral mRNA synthesis occurred. When their infiltration was slow, ephemeral foci of replication were identified. These findings were paralleled by plasma viremia; low viremia coincided with rapid sterilization of the delayed type hypersensitivity sites, whereas high load was found in association with local replication and delayed sterilization. These data suggest that although effective local control of SIV is possible once antiviral T lymphocytes have arrived on site, the slower deployment of these T cells may allow the virus to escape and thus to reseed the pool of memory T cells.
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, 28, Rue du Dr. Roux, 75724 Paris Cedex 15, France.
authors.forename: P
authors.initials: P
authors.lastname: Blancou
authors.forename: N
authors.initials: N
authors.lastname: Chenciner
authors.forename: M C
authors.initials: MC
authors.lastname: Cumont
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
authors.forename: B
authors.initials: B
authors.lastname: Hurtrel
authors.forename: R
authors.initials: R
authors.lastname: Cheynier
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D006968:Hypersensitivity, Delayed; D017403:In Situ Hybridization; D008253:Macaca mulatta; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers