title: Biochemical characterization of glucosylglycerol-phosphate synthase of Synechocystis sp. strain PCC 6803: comparison of crude, purified, and recombinant enzymes.
abstract: Glucosylglycerol-phosphate synthase (GGPS), the key enzyme of the glucosylglycerol biosynthesis in salt-stressed cells of Synechocystis, was biochemically analyzed in crude extracts, after partial purification by FPLC and after overexpression of the gene ggpS in Escherichia coli and purification to homogenity of the recombinant protein, respectively. These GGPS preparations behaved similarly with regard to temperature stability, pH optimum, Mg2+ dependence, inhibition by phosphates, and Km values, but differed in their dependence on NaCl concentration: crude enzyme needed activation by addition of NaCl, whereas both partially-purified and recombinant GGPS showed high activities independent of the NaCl concentration.
authors.affiliation: Department of Biology, University of Rostock, Germany. martin.hagemann@biologie.uni-rostock.de
authors.forename: M
authors.initials: M
authors.lastname: Hagemann
authors.forename: U
authors.initials: U
authors.lastname: Effmert
authors.forename: T
authors.initials: T
authors.lastname: Kerstan
authors.forename: A
authors.initials: A
authors.lastname: Schoor
authors.forename: N
authors.initials: N
authors.lastname: Erdmann
mesh_terms: D001426:Bacterial Proteins; D003470:Culture Media; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D015964:Gene Expression Regulation, Bacterial; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D012965:Sodium Chloride; D005964:Glucosyltransferases; C400922:glucosylglycerol-phosphate synthase