title: Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors--theoretical and practical considerations.
abstract: Theoretical and practical insights into the design and development of immobilised enzyme inhibition biosensors are reported. A general mathematical expression relating the percent of enzyme inhibition (i.e. the analytical signal) to both the inhibitor concentration and the incubation time is presented. The relevant physical, chemical and biochemical parameters required by the model are developed and discussed in terms of the inhibition of acetylcholinesterase by the organophosphorous pesticide, paraoxon. A second enzyme, choline oxidase and an amperometric transducer are used to facilitate the determination acetylcholinesterase inhibitor.
authors.affiliation: School of Environmental and Applied Sciences, Griffith University, Gold Coast Campus, PMB 50, Gold Coast Mail Centre, Queensland 9726, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: R
authors.initials: R
authors.lastname: John
mesh_terms: D000110:Acetylcholinesterase; D000429:Alcohol Oxidoreductases; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D002800:Cholinesterase Inhibitors / Q000032:analysis; D004563:Electrochemistry; D004785:Environmental Pollutants / Q000032:analysis; D004800:Enzymes, Immobilized; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis; D008962:Models, Theoretical; D010261:Paraoxon / Q000032:analysis; D014159:Transducers
chemical_list: D002800:Cholinesterase Inhibitors; D004785:Environmental Pollutants; D004800:Enzymes, Immobilized; D007306:Insecticides; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; D010261:Paraoxon