Characterization and chromosome location of satellite DNA in the leaf beetle Chrysolina americana (Coleoptera, Chrysomelidae).
This paper is the first record of the satellite DNA of the specialized phytophagous genus Chrysolina. The satellite DNA of Chrysolina americana is organized in a tandem repeat of monomers 189 bp long, has a A + T content of 59.6% and presents direct and inverted internal repeats. Restriction analysis of the total DNA with methylation sensitive enzymes suggests that this repetitive DNA is undermethylated. In siti hybridization with a biotinylated probe of the satellite DNA showed the pericentromeric localization of these sequences in all meiotic bivalents. The presence of this repetitive DNA in other species of the genus was also tested by Southern analysis. The results showed that this satellite DNA sequence is specific to the C. americana genome and has not been found in three other species of Chrysolina with a different choice of host plants than in the former.
Lorite
P
P
Area de Genética, Departmento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Spain. plorite@ujaen.es
Palomeque
T
T
Garnería
I
I
Petitpierre
E
E
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D001517:Coleoptera / Q000235:genetics*; D016384:Consensus Sequence; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D013045:Species Specificity; D020080:Tandem Repeat Sequences
D004276:DNA, Satellite