Biological effects of a nano red elemental selenium.
A novel selenium form, nano red elemental selenium (Nano-Se) was prepared by adding bovine serum albumin to the redox system of selenite and glutathione. Nano-Se has a 7-fold lower acute toxicity than sodium selenite in mice (LD(50) 113 and 15 mg Se/kg body weight respectively). In Se-deficient rat, both Nano-Se and selenite can increase tissue selenium and GPx activity. The biological activities of Nano-Se and selenite were compared in terms of cell proliferation, enzyme induction and protection against free racial-mediated damage in human hepatoma HepG2 cells. Nano-Se and selenite are similarly cell growth inhibited and stimulated synthesis of glutathione peroxidase (GPx), phospholipid hydroperoxide glutathione peroxidase (PHGPx) and thioredoxin reductase (TR). When HepG2 cells were co-treated with selenium and glutathione, Nano-Se showed less pro-oxidative effects than selenite, as measured by cell growth. These results demonstrate that Nano-Se has a similar bioavailability in the rat and antioxidant effects on cells.
Zhang
J S
JS
Institute of Solid Physics, Chinese Academy of Science, Hefei, 230031, P.R. China.
Gao
X Y
XY
Zhang
L D
LD
Bao
Y P
YP
D001682:Biological Availability; D006528:Carcinoma, Hepatocellular; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008113:Liver Neoplasms; D010269:Paraquat / Q000494:pharmacology; D012643:Selenium / Q000172:deficiency / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D018038:Sodium Selenite / Q000633:toxicity; D013880:Thioredoxin-Disulfide Reductase / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D005609:Free Radicals; D006131:Growth Inhibitors; D005979:Glutathione Peroxidase; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite; D010269:Paraquat