title: Nicotine toxicity to the ultrastructure of the testis in rats.
abstract: OBJECTIVE
  To investigate the influence of nicotine exposure on the ultrastructure of the rat testis.
  
  
  METHODS
  Twenty rats were injected with nicotine at a dose of 0.4 mg/100 g body weight daily for 3 months; a group of 20 control rats matched for weight and age were injected with saline only for the same duration. The testes were then harvested and examined by transmission electron microscopy.
  
  
  RESULTS
  Rats given nicotine showed: thickening of the tunica propria, caused by an increase in the collagen fibres under the irregular basal lamina; degeneration of junctional specializations between the Sertoli cells, with malformed nuclei showing condensed chromatin; Sertoli cells with numerous polymorphic mitochondria with irregular cristae and an electron-dense matrix. The germ cells were degenerated, spermatids retained excess cytoplasm and accumulated electron-dense lipid droplets in the cytoplasm. The acrosomes were irregular and abnormally configured.
  
  
  CONCLUSIONS
  There were ultrastructural alterations in rats exposed to nicotine that could be attributed to the detrimental effects of nicotine on germ cells, peritubular structures and Sertoli cells.
authors.affiliation: Department of Urology, Histology and Embryology, and Physiology, School of Medicine, University of Ankara, Turkey. ksaydos@superonline.com
authors.forename: K
authors.initials: K
authors.lastname: Aydos
authors.forename: M C
authors.initials: MC
authors.lastname: Güven
authors.forename: B
authors.initials: B
authors.lastname: Can
authors.forename: A
authors.initials: A
authors.lastname: Ergün
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D007248:Infertility, Male / Q000139:chemically induced*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009538:Nicotine / Q000633:toxicity*; D018722:Nicotinic Agonists / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D018722:Nicotinic Agonists; D009538:Nicotine