title: TENB2, a proteoglycan identified in prostate cancer that is associated with disease progression and androgen independence.
abstract: TENB2 encodes a putative transmembrane proteoglycan, related to the EGF/heregulin family of growth factors and follistatin, which has been identified through the application of a differential display technique to a xenograft model of prostate cancer. Northern analysis and competitive PCR were used to demonstrate significantly increased TENB2 expression (p = 0.0003) on the acquisition of androgen independence in the model system. TENB2 is also overexpressed in clinical prostate carcinoma vs. its benign counterpart (p < 0.0001), with particular prominence in high-grade tumours, and shows a high degree of tissue specificity, being detected on a multitissue Northern array exclusively in brain and prostate material. Studies of recombinant protein expression demonstrate that TENB2 is a chondroitin sulphate proteoglycan. The presence of an EGF and 2 follistatin domains suggests a role in the regulation of growth factor signalling either as a ligand precursor, a membrane-bound receptor or as a binding protein for growth factors. These data are indicative of a significant role for TENB2 in the progression of poorly differentiated tumour types, with implications for prostate cancer detection, prognosis and therapy.
authors.affiliation: Tenovus Cancer Research Centre, Welsh School of Pharmacy, Cardiff University, Wales, United Kingdom. evegj@hotmail.com
authors.forename: E
authors.initials: E
authors.lastname: Glynne-Jones
authors.forename: M E
authors.initials: ME
authors.lastname: Harper
authors.forename: L T
authors.initials: LT
authors.lastname: Seery
authors.forename: R
authors.initials: R
authors.lastname: James
authors.forename: I
authors.initials: I
authors.lastname: Anglin
authors.forename: H E
authors.initials: HE
authors.lastname: Morgan
authors.forename: K M
authors.initials: KM
authors.lastname: Taylor
authors.forename: J M
authors.initials: JM
authors.lastname: Gee
authors.forename: R I
authors.initials: RI
authors.lastname: Nicholson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002809:Chondroitin Sulfates / Q000032:analysis; D006224:Cricetinae; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C038142:chondroitin sulfate glycosaminoglycan; D002809:Chondroitin Sulfates