Prion peptide 106-126 modulates the aggregation of cellular prion protein and induces the synthesis of potentially neurotoxic transmembrane PrP.
In infectious and familial prion disorders, neurodegeneration is often seen without obvious deposits of the scrapie prion protein (PrP(Sc)), the principal cause of neuronal death in prion disorders. In such cases, neurotoxicity must be mediated by alternative pathways of cell death. One such pathway is through a transmembrane form of PrP. We have investigated the relationship between intracellular accumulation of prion protein aggregates and the consequent up-regulation of transmembrane prion protein in a cell model. Here, we report that exposure of neuroblastoma cells to the prion peptide 106-126 catalyzes the aggregation of cellular prion protein to a weakly proteinase K-resistant form and induces the synthesis of transmembrane prion protein, the proposed mediator of neurotoxicity in certain prion disorders. The N terminus of newly synthesized transmembrane prion protein is cleaved spontaneously on the cytosolic face of the endoplasmic reticulum, and the truncated C-terminal fragment accumulates on the cell surface. Our results suggest that neurotoxicity in prion disorders is mediated by a complex pathway involving transmembrane prion protein and not by deposits of aggregated and proteinase K-resistant PrP alone.
Gu
Yaping
Y
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
Fujioka
Hisashi
H
Mishra
Ravi Shankar
RS
Li
Ruliang
R
Singh
Neena
N
D000595:Amino Acid Sequence; D000682:Amyloid / Q000378:metabolism; D002384:Catalysis; D003902:Detergents; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000502:physiology*; D011328:Prions / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D014407:Tumor Cells, Cultured
D000682:Amyloid; D003902:Detergents; D010446:Peptide Fragments; D011328:Prions; C082632:prion protein (106-126)