Amphiphile-induced vesiculation in aged hereditary spherocytosis erythrocytes indicates normal membrane stability properties under non-starving conditions.
Aged HS erythrocytes with a defined primary defect in band 3 protein or ankyrin were incubated with amphiphiles (detergents) at sublytic concentrations (37 C, 60 min) or glucose-starved (37 C, 24 h). In line with previous studies, the release of AChE (exovesicles) from HS erythrocytes during glucose-starvation was significantly higher (11%) compared to that from control erythrocytes (1%). Control and HS cells responded, however, similarly to amphiphile-treatment (non-starving conditions). Amphiphiles induced similar types of shape alterations and a similar amount of AChE release (14-15%). Furthermore, the size and shape of amphiphile-induced exo- and endovesicles released from control and HS erythrocytes were similar. The results suggest that the stability properties of the membrane are not seriously disturbed in aged HS erythrocytes under non-starving conditions.
Hägerstrand
H
H
Department of Biology, Abo Akademi University, Abo-Turku, Finland. henry.hagerstrand@abo.fi
Iglic
A
A
Bobrowska-Hägerstrand
M
M
Lindqvist
C
C
Isomaa
B
B
Eber
S
S
D017920:Antiporters / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D048429:Cell Size / Q000187:drug effects; D016922:Cellular Senescence / Q000502:physiology*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005508:Food Deprivation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009996:Osmotic Fragility; D022142:Secretory Vesicles / Q000187:drug effects; D013103:Spherocytosis, Hereditary / Q000097:blood* / Q000473:pathology; D013501:Surface-Active Agents / Q000494:pharmacology*
D017920:Antiporters; D013501:Surface-Active Agents; D005947:Glucose