title: Characterization of two new alleles at the goat CSN1S2 locus.
abstract: Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is characterized by a G --> A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2(D) allele, apparently associated with a decreased synthesis of alpha s2-casein, is characterized by a 106-bp deletion, involving the last 11 bp of the exon 11 and the first 95 bp of the following intron. Methods (PCR-RFLP and PCR) for identification of carriers of these alleles have been developed.
authors.affiliation: Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università degli Studi di Napoli Federico II, Napoli, Italy. rammuno@unina.it
authors.forename: L
authors.initials: L
authors.lastname: Ramunno
authors.forename: G
authors.initials: G
authors.lastname: Cosenza
authors.forename: M
authors.initials: M
authors.lastname: Pappalardo
authors.forename: E
authors.initials: E
authors.lastname: Longobardi
authors.forename: D
authors.initials: D
authors.lastname: Gallo
authors.forename: N
authors.initials: N
authors.lastname: Pastore
authors.forename: P
authors.initials: P
authors.lastname: Di Gregorio
authors.forename: A
authors.initials: A
authors.lastname: Rando
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D004247:DNA; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006041:Goats / Q000235:genetics*; D008969:Molecular Sequence Data; D017384:Sequence Deletion
chemical_list: D002364:Caseins; D004247:DNA