title: Interaction of the antiactivator FleN with the transcriptional activator FleQ regulates flagellar number in Pseudomonas aeruginosa.
abstract: Flagellar number in Pseudomonas aeruginosa is controlled by FleN, a putative ATP/GTP binding protein. Disruption of fleN results in multiflagellation of the otherwise monoflagellate strains PAK and PAO1 and is associated with a chemotactic defect. We propose that flagellar number is maintained by the antiactivator FleN, which downregulates flagellar genes by binding to their transcriptional activator, FleQ, an enhancer binding protein belonging to the NifA subfamily. In this report we demonstrate direct interaction of FleN and FleQ in the yeast two-hybrid system. Mutagenesis of the putative ATP/GTP binding motif in FleN(24K-->Q) and truncation of FleN at either the N or C terminus abrogates this interaction. FleN does not inhibit the DNA binding ability of FleQ in vitro, thus indicating that it probably utilizes another mechanism(s) to serve as a FleQ antiactivator.
authors.affiliation: Department of Medicine/Infectious Diseases, University of Florida, Gainesville 32610, USA.
authors.forename: N
authors.initials: N
authors.lastname: Dasgupta
authors.forename: R
authors.initials: R
authors.lastname: Ramphal
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005407:Flagella / Q000378:metabolism*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C108098:FleQ protein, Pseudomonas aeruginosa; D015534:Trans-Activators; C403326:fleN protein, Pseudomonas aeruginosa