Characterization of glycosaminoglycans from human normal and scoliotic nasal cartilage with particular reference to dermatan sulfate.
The composition and the distribution of glycosaminoglycans (GAGs) present in normal human nasal cartilage (HNNC), were examined and compared with those in human scoliotic nasal cartilage (HSNC). In both tissues, hyaluronan (HA), keratan sulfate (KS) and the galactosaminoglycans (GalAGs)--chondroitin sulfate (CS) and dermatan sulfate (DS)--were identified. The overall GAG content in HSNC was approx. 30% higher than the HNNC. Particularly, a 114% increase in HA, and 46% and 86% in KS and DS, respectively, was recorded. CS was the main type of GAG in both tissues with no significant compositional difference. GalAG chains in HSNC exhibited an altered disaccharide composition which was associated with significant increases of non-sulfated and 6-sulfated disaccharides. DS, which was identified and quantitated for the first time in HNNC and HSNC, contained low amounts of iduronic acid (IdoA), 18% and 28% respectively. In contrast to other tissues, where IdoA residues are organized in long IdoA rich repeats, the IdoA residues of DS in human nasal cartilage seemed to be randomly distributed along the chain. DS chains in HSNC were of larger average molecular size than those from HNNC. These results clearly indicate the GAG content and pattern in both HNNC and HSNC and demonstrate that scoliosis of nasal septum cartilage is related to quantitative and structural modifications at the GAG level.
Theocharis
A D
AD
Laboratory of Biochemistry, Department of Chemistry, School of Medicine, University of Patras, Greece.
Tsara
M E
ME
Papageorgakopoulou
N
N
Vynios
D H
DH
Theocharis
D A
DA
D000328:Adult; D005591:Chemical Fractionation; D019765:Chondroitin ABC Lyase; D002850:Chromatography, Gel; D003871:Dermatan Sulfate / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D009300:Nasal Septum / Q000002:abnormalities* / Q000737:chemistry / Q000378:metabolism
D004187:Disaccharides; D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D019765:Chondroitin ABC Lyase