title: Analogs of Eap protein are conserved and prevalent in clinical Staphylococcus aureus isolates.
abstract: Map and Eap are secreted Staphylococcus aureus proteins that interact with various extracellular matrix molecules. PCR analysis using map primers yielded positive reactions in 97.9% of S. aureus isolates but not in Staphylococcus epidermidis isolates. Cloning and sequencing of the conferring genes revealed a high degree of overall homology combined with size variability of the gene product due to various repeat numbers and early translation termination in a poly(A) region. Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing.
authors.affiliation: Institute of Medical Microbiology, University of Muenster, Muenster, Germany. muzaffa@uni-muenster.de
authors.forename: M
authors.initials: M
authors.lastname: Hussain
authors.forename: K
authors.initials: K
authors.lastname: Becker
authors.forename: C
authors.initials: C
authors.lastname: von Eiff
authors.forename: G
authors.initials: G
authors.lastname: Peters
authors.forename: M
authors.initials: M
authors.lastname: Herrmann
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016601:RNA-Binding Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*; D013212:Staphylococcus epidermidis
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; C444739:Eap-N protein, Staphylococcus aureus; D016601:RNA-Binding Proteins