Genome size in Arachis duranensis: a critical study.
Arachis duranensis is a diploid wild relative of the tetraploid cultivated peanut Arachis hypogaea. The literature indicates two 2C genomic DNA mean values (genome size) for A. duranensis, 4.92 and 5.64 pg, and intraspecific variation of up to 11% negatively correlated with altitude above sea level of the collection sites has been reported. Our recent investigations of Arachis species have shown that unrecognized technical problems with peanut material may have influenced previous genome-size data and rendered them open to critical comments. In the present study, 20 accessions of A. duranensis were investigated by means of DNA flow cytometry (propidium iodide staining) and several of these also by Feulgen DNA image analysis. Pisum sativum was used as the internal standard (2C = 8.84 pg). 2C values in A. duranensis were about half those described previously and varied between 2.49 and 2.87 pg (flow cytometry). This variation was statistically significant and reproducible. There was a negative correlation of genome size with latitude and altitude above sea level of the collection sites. Such a correlation had been already found in one of the previous studies. However, the incongruences between the absolute DNA content values obtained in the present investigation and those in the literature point to the importance of carrying out methodological studies on best practice in DNA-content determinations in plants.
Temsch
E M
EM
Institute of Botany of the University of Vienna, Austria. temsch@s1.botanik.univie.ac.at
Greilhuber
J
J
D010367:Arachis / Q000235:genetics*; D004396:Coloring Agents; D003720:Densitometry; D005434:Flow Cytometry; D014644:Genetic Variation; D018745:Genome, Plant; D012394:Rosaniline Dyes; D013020:South America
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes