Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.
Higgins
J A
JA
U.S. Department of Agriculture-Agricultural Research Service, Beltsville, Maryland 20705, USA. jhiggins@anri.barc.usda.gov
Jenkins
M C
MC
Shelton
D R
DR
Fayer
R
R
Karns
J S
JS
D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics* / Q000254:growth & development; D004269:DNA, Bacterial / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000302:isolation & purification*; D019453:Escherichia coli O157 / Q000235:genetics* / Q000254:growth & development; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D016054:DNA, Protozoan