The cytoskeleton of hydatid cyst cultured cells and its sensitivity to inhibitors.
Cell cultures obtained from the germinal layer of hydatid cysts of the parasitic tapeworm Echinococcus granulosus were characterized with respect to their microtubule and microfilament systems. These were stained using monospecific antibodies against tubulin from sea urchin spermatozoa or sheep brain and against Dictyostelium discoideum actin as well as rhodamine conjugated phalloidin. The results show that the distribution of microtubules nad actin containing fibres of these cells is remarkably similar to that of mammalian cells both during interphase and mitosis. Hydatid cells, however, could not be stained with a specific antivimentin antibody. Indirect immunofluorescence with antitubulin antibodies of inhibitor treated cells shows that hydatid cell microtubules are sensitive to several antimicrotubular drugs including benzimidazole derivatives, colchicine, vinblastine, and griseofulvin.
Rubino
S
S
Institute of Medical Microbiology, University of Sassari, Italy
Fiori
P L
PL
Lubinu
G
G
Monaco
G
G
Cappuccinelli
P
P
D000199:Actins / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003599:Cytoskeleton / Q000737:chemistry*; D004443:Echinococcosis / Q000188:drug therapy*; D019084:Fluorescent Antibody Technique, Indirect; D006074:Gout Suppressants / Q000494:pharmacology; D006118:Griseofulvin / Q000494:pharmacology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D012617:Sea Urchins; D012756:Sheep; D013094:Spermatozoa / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry; D014747:Vinblastine / Q000494:pharmacology
D000199:Actins; D000906:Antibodies; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D006074:Gout Suppressants; D014404:Tubulin; D006118:Griseofulvin; D014747:Vinblastine; D003078:Colchicine