Tumor cells as the origin of elevated serum alpha1,3fucosyltransferase in association with malignancy.
We have previously reported that the elevated activities of serum alpha 1,3fucosyltransferase reverted to normal levels after curative removal of the tumors. To determine the origin of elevated serum alpha 1,3fucosyltransferase, blood samples were obtained from both the drainage vein and the artery in patients with different stages of colorectal cancer at surgery. The enzyme levels in all samples from the drainage vein were found to be higher than the levels in the artery that fed the tumor. Hence, the origin of elevated alpha1,3fucosyltransferase in serum was thought to be the tumor rather than the liver that is the normal source of serum alpha1,3fucosyltransferase. When serum samples not only from colorectal cancer patients but also from patients with gastric, liver, lung, pancreas, bladder and esophagus cancer were treated with anti-FUTVI antibody, the measured activities of alpha1,3fucosyltransferase were markedly reduced. Further, secretion of alpha1,3fucosyltransferase from human colorectal carcinoma cells was also detected in the culture medium by Western immuno-blot analysis with anti-FUTVI antibody.
Asao
T
T
First Department of Surgery, Gunma University School of Medicine, Maebashi, Japan. asaot@med.gunma-u.ac.jp
Kuwano
H
H
Nakamura
J
J
Okamura
A
A
Berger
E G
EG
Matta
K L
KL
Yazawa
S
S
D000368:Aged; D000906:Antibodies / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western; D002455:Cell Division; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology; D005260:Female; D005647:Fucosyltransferases / Q000097:blood* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D000906:Antibodies; D014408:Biomarkers, Tumor; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase