Chromosome studies of murine T-cell lymphoid leukemia and derived cell lines.
Several cell lines were previously established from a spontaneous murine T-cell leukemia (LB). The aim of this study was to analyze the G- and C-banded karyotypes of the parental LB tumor cells and the derived cell lines. A sensitive cell line (LBL) from which two sublines originated, as well as Vincristine (LBR-V160) and Doxorubicin (LBR-D160) resistant cell lines, were used. Our results showed that LB cells had a pseudo-diploid karyotype with 40 acrocentric chromosomes in which trisomy of chromosome 14 was the most relevant alteration. The sensitive cell line showed this alteration in all metaphases studied; no changes in karyotypes were observed in either subline, despite their dissimilar morphology and growth patterns. In contrast, both resistant lines displayed a more heterogeneous karyotype with no common markers, except for the finding that chromosome 5 was involved in a trisomy in LBR-V160 and in a translocation with chromosome 12 in LBR-D160. Taking into account that the mdr genes are located in chromosome 5, these results suggest a possible association between such alterations and the acquisition of drug resistance.
Fabris
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V
Centro de Investigaciones en Reproducción (CIR), Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 10 (C1121ABG), Buenos Aires, Argentina. cir@fmed.uba.ar
Ernst
G
G
Lopes
E C
EC
García
M
M
Hajos
S
S
Alvarez
E
E
Merani
S
S
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D002871:Chromosome Banding; D017931:DNA Primers; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D007621:Karyotyping; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D017931:DNA Primers; D014750:Vincristine; D004317:Doxorubicin