A comparison of the stage-specific efficacy of chloroquine, artemether and dioncophylline B against the rodent malaria parasite Plasmodium chabaudi chabaudi in vivo.
Chloroquine, artemether and dioncophylline B efficacy against Plasmodium chabaudi was compared. One intraperitoneal injection (10 mg/kg body weight) was given daily over 3 consecutive days to OF1 mice when they were predominantly bearing ring, trophozoite and schizont forms. The parasitaemia was monitored every 2 h during two schizogonic cycles and daily thereafter until parasites were cleared. Chloroquine was more efficient at the trophozoite stage, while artemether was effective against all erythrocytic stages, with a marked efficacy against the trophozoite stage. Chloroquine-treated and artemether-treated parasites displayed a pigment-clumping morphology and lowered the parasitaemia faster than dioncophylline B. Dioncophylline B was effective at trophozoite and schizont stages, but completely ineffective at the ring stage. These results demonstrate that a better timing of drug administration increases the efficacy of common and new antimalarial drugs and provides a model for antimalarial-action monitoring. Drug-induced changes in infected erythrocyte morphology are presented.
Chimanuka
B
B
Vakgroep Farmaceutische Technologie en Fysische Farmacie, Vrije Universiteit Brussel, Brussels, Belgium. chimbant@minf.vub.ac.be
François
G
G
Timperman
G
G
Heyden
Y V
YV
Holenz
J
J
Plaizier-Vercammen
J
J
Bringmann
G
G
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology / Q000627:therapeutic use*; D000077549:Artemether; D037621:Artemisinins; D002738:Chloroquine / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D007546:Isoquinolines / Q000494:pharmacology / Q000627:therapeutic use*; D008018:Life Cycle Stages / Q000187:drug effects; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology; D051379:Mice; D018512:Parasitemia / Q000469:parasitology; D016788:Plasmodium chabaudi / Q000187:drug effects* / Q000254:growth & development*; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
D000962:Antimalarials; D037621:Artemisinins; D007546:Isoquinolines; D012717:Sesquiterpenes; C402882:dioncophylline B; D002738:Chloroquine; D000077549:Artemether