Exploring the recognized bio-mimicry materials for gas sensing.
This study was undertaken to synthesize peptides that are partially similar to the binding sites of human olfactory receptor protein. First, a putative 3-D model structure of human olfactory receptor protein (P30953) was modeled using a molecular simulation method. The computer docking simulation was then performed to determine the most plausible binding sites between the model structure and target gases, trimethylamine, ammonia, acetic acid, and o-xylene. According to the simulation result, a series of polypeptide sequences, horp61 for TMA, horp103 for o-xylene, horp109 for ammonia, and horp193 for acetic acid as recognized molecules were designed for gas sensing purposes. Preparing these peptides as corresponding gas sensing probes, the results showed a high relative sensitivity response of 6.7 for TMA (probe horp61), 5.1 for o-xylene (probe horp103), 11 for ammonia (probe horp109), and 28 for acetic acid (probe horp193), respectively. These results indicate that peptide mimicking of binding domain on olfactory receptor opens a new window and offers a novel strategy for the further development of recognized materials for gas sensing.
Wu
T Z
TZ
Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, ROC. tzwu@mail.ndhu.edu.tw
Lo
Y R
YR
Chan
E C
EC
D000595:Amino Acid Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D005740:Gases / Q000032:analysis*; D006801:Humans; D008958:Models, Molecular; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D018035:Receptors, Odorant / Q000737:chemistry / Q000378:metabolism
D005740:Gases; D010455:Peptides; D018035:Receptors, Odorant