Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells.
Subcellular localization of the Brome mosaic virus replicase-related 1a and 2a proteins, and the 3a movement protein in infected barley leaves was examined by immunogold electron microscopy. The 1a and 2a proteins colocalized at infection-specific electron-dense cytoplasmic inclusions. The 3a protein was also detected in these inclusions. The inclusions were oval or amorphous, and contained electron-lucent regions. Electron microscopic autoradiography showed that the inclusions were sites of [3H]uridine incorporation, suggesting that they are sites of viral RNA synthesis.
Dohi
K
K
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Japan.
Mori
M
M
Furusawa
I
I
Mise
K
K
Okuno
T
T
D017795:Bromovirus / Q000378:metabolism / Q000502:physiology*; D001467:Hordeum / Q000648:ultrastructure / Q000821:virology*; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000378:metabolism*; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D053830:Plant Viral Movement Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase