Cell-surface proteoglycan expression by lymphocytes from peripheral blood and gingiva in health and periodontal disease.
Cell-surface proteoglycans are involved in lymphocyte migration and activation. This study investigated the expression of syndecan-1, syndecan-4, and glypican in peripheral blood lymphocytes and by lymphocytes in variously inflamed periodontal tissues. Gingival specimens from healthy, gingivitis, or chronic periodontitis sites were stained by means of antibodies against B- and T-lymphocytes and also syndecan-1, syndecan-4, and glypican. Syndecan-1 expression by peripheral blood mononuclear cells (PBMC) from healthy, gingivitis, and chronic periodontitis subjects was assessed by flow cytometry. Syndecan-1 was expressed by B-cells/plasma cells but not T-cells in both gingivitis and chronic periodontitis lesions. Both B-cells/plasma cells and T-cells in gingivitis and chronic periodontitis expressed syndecan-4. Glypican was expressed only by macrophages. Stimulation of PBMC with mitogens and growth factors modulated syndecan-1 expression in both the T- and B-cells. Thus, cell-surface proteoglycan expression by lymphocytes in periodontal inflammation is cell-type-specific and may be modulated by inflammation.
Manakil
J F
JF
School of Dentistry, The University of Queensland, Brisbane, Australia.
Sugerman
P B
PB
Li
H
H
Seymour
G J
GJ
Bartold
P M
PM
D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000378:metabolism / Q000473:pathology; D000704:Analysis of Variance; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D002908:Chronic Disease; D005260:Female; D005434:Flow Cytometry; D005881:Gingiva / Q000378:metabolism / Q000473:pathology*; D005884:Gingival Hemorrhage / Q000378:metabolism / Q000473:pathology; D005891:Gingivitis / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D006133:Growth Substances / Q000494:pharmacology; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D017622:Periodontal Attachment Loss / Q000378:metabolism / Q000473:pathology; D010514:Periodontal Pocket / Q000378:metabolism / Q000473:pathology; D010518:Periodontitis / Q000097:blood / Q000378:metabolism / Q000473:pathology*; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology; D011509:Proteoglycans / Q000032:analysis*; D012044:Regression Analysis; D013223:Statistics as Topic; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D019237:Tooth Cervix / Q000473:pathology
D006133:Growth Substances; D019812:Heparan Sulfate Proteoglycans; D008562:Membrane Glycoproteins; D008934:Mitogens; D011509:Proteoglycans; C508241:SDC1 protein, human; C508304:SDC4 protein, human; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans