A method to obtain right-side-out and sealed plasma membrane vesicles from the filamentous fungus Penicillium simplicissimum.
Plasma membrane vesicles were purified from the citrate excreting filamentous fungus Penicillium simplicissimum. The hyphae were disrupted by grinding in a glass bead mill and plasma membranes were obtained from a crude membrane fraction by partitioning in an aqueous dextran/polyethylene glycol two-phase system. The resulting enriched plasma membrane fraction contained only minor contaminations of marker enzymes of intracellular membranes, consisted of more than 90% of right-side-out vesicles and was sealed for potassium.
Müller
B
B
Institute of Microbiology, Technikerstrasse 25, 6020 Innsbruck, Austria.
Zanella
A
A
Burgstaller
W
W
D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000648:ultrastructure*; D002951:Citrates / Q000378:metabolism; D007425:Intracellular Membranes; D008854:Microscopy, Electron; D010407:Penicillium / Q000378:metabolism / Q000648:ultrastructure*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D022161:Transport Vesicles / Q000648:ultrastructure*
D002951:Citrates; D006180:Proton-Translocating ATPases