title: Evidence for a nuclear passage of nascent polypeptide-associated complex subunits in yeast.
abstract: The nascent polypeptide-associated complex (NAC) has been found quantitatively associated with ribosomes in the cytosol by means of cell fractionation or fluorescence microscopy. There have been reports, however, that single NAC subunits may be involved in transcriptional regulation. We reasoned that the cytosolic location might only reflect a steady state equilibrium and therefore investigated the yeast NAC proteins for their ability to enter the nucleus. We found that single subunits of yeast NAC can indeed be transported into the nucleus and that this transport is an active process depending on different nuclear import factors. Translocation into the nucleus was only observed when binding to ribosomes was inhibited. We identified a domain of the ribosome-binding NAC subunit essential for nuclear import via the importin Kapl23p/Pselp-dependent import route. We hypothesize that newly translated NAC proteins travel into the nucleus to bind stoichiometrically to ribosomal subunits and then leave the nucleus together with these subunits to concentrate in the cytosol.
authors.affiliation: Max Delbrück Center for Molecular Medicine, Berlin, Germany
authors.forename: J
authors.initials: J
authors.lastname: Franke
authors.forename: B
authors.initials: B
authors.lastname: Reimann
authors.forename: E
authors.initials: E
authors.lastname: Hartmann
authors.forename: M
authors.initials: M
authors.lastname: Köhlerl
authors.forename: B
authors.initials: B
authors.lastname: Wiedmann
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology; D017353:Gene Deletion; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; D016296:Mutagenesis / Q000502:physiology; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D018271:Signal Recognition Particle / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D028901:alpha Karyopherins / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C078340:EGD1 protein, S cerevisiae; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; C483822:PSE1 protein, S cerevisiae; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D018271:Signal Recognition Particle; D015534:Trans-Activators; D014157:Transcription Factors; D028901:alpha Karyopherins; C087978:karyopherin alpha 2; C096099:nascent-polypeptide-associated complex; D049452:Green Fluorescent Proteins