title: Proliferation rate of rabbit corneal epithelium during overnight rigid contact lens wear.
abstract: OBJECTIVE
  To examine cell proliferation of the normal corneal epithelium and during extended rigid gas-permeable (RGP) lens wear.
  
  
  METHODS
  Twenty-three New Zealand White rabbits were fitted unilaterally with either a low oxygen transmissible (Dk/t) or hyper-Dk/t RGP lens, with the other eye serving as a control. The rabbits were injected with 5-bromo-2-deoxyuridine (BrdU) 24-hours later and killed at three time points: 1, 3, and 7 days after injection. Corneas were processed for immunocytochemistry, and sequential digital images were taken from the superior limbus to the central epithelium with an epifluorescence microscope. The total number of BrdU-labeled cell pairs was quantified.
  
  
  RESULTS
  The limbus in normal corneas was significantly less populated with BrdU-labeled cells than the central and peripheral epithelium (P < 0.05). The peripheral epithelium adjacent to the limbus was marked by a peak of labeled cells (P < 0.05). Both types of RGP lenses produced an increase in BrdU labeling in the limbus and a dramatic decrease in the central epithelium (80% for low Dk/t, 37% for hyper Dk/t). At day 3 and 7 after BrdU injection, the low-Dk/t lens continued to show decreased BrdU labeling centrally, whereas the limbus remained increased. Hyper-Dk/t lens wear however, showed persistent limbal elevation but equivalent numbers of BrdU-labeled cells centrally at days 3 and 7, compared with control corneas. Keratocytes unexpectedly showed BrdU labeling during RGP lens wear.
  
  
  CONCLUSIONS
  Limbus, peripheral, and central epithelium were characterized by different proliferation rates in the normal rabbit cornea. RGP lens wear significantly altered the homeostatic proliferation pattern of the epithelium with the low-Dk/t lens having the most dramatic effect. RGP contact lens wear appears to stimulate proliferation of keratocytes.
authors.affiliation: Department of Ophthalmology, The University of Texas Southwestern Medical Center at Dallas, 75039-9057, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Ladage
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: D H
authors.initials: DH
authors.lastname: Ren
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: J V
authors.initials: JV
authors.lastname: Jester
authors.forename: W M
authors.initials: WM
authors.lastname: Petroll
authors.forename: J P
authors.initials: JP
authors.lastname: Bergmanson
authors.forename: H D
authors.initials: HD
authors.lastname: Cavanagh
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D003261:Contact Lenses; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D019573:Epithelium, Corneal / Q000166:cytology* / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D008856:Microscopy, Fluorescence; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine; D010100:Oxygen