title: Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: factors regulating the protein release.
abstract: A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility.
authors.affiliation: Department of Chemistry, Moscow State University, Vorob'evy Gory, Moscow, 119899, Russia.
authors.forename: N G
authors.initials: NG
authors.lastname: Balabushevitch
authors.forename: G B
authors.initials: GB
authors.lastname: Sukhorukov
authors.forename: N A
authors.initials: NA
authors.lastname: Moroz
authors.forename: D V
authors.initials: DV
authors.lastname: Volodkin
authors.forename: N I
authors.initials: NI
authors.lastname: Larionova
authors.forename: E
authors.initials: E
authors.lastname: Donath
authors.forename: H
authors.initials: H
authors.lastname: Mohwald
mesh_terms: D000327:Adsorption; D002214:Capsules / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism
chemical_list: D002214:Capsules; D014361:Trypsin Inhibitors; D002918:Chymotrypsin