Iron tetrakis (n-methyl-4'-pyridyl) porphyrinato (FeTMPyP) is a potent scavenging antioxidant and an inhibitor of stimulant-induced NF-kappaB activation of raw 264.7 macrophages.
Metalloporphyrins have been shown to be protective in oxidative stress models. However, the molecular basis for the antioxidative and antiinflammatory activities of iron tetrakis (N-methyl-4'-pyridyl) porphyrinato (FeTMPyP) is not known. The objective of this study was to determine whether FeTMPyP exhibited the ability to (1) scavenge reactive oxygen species (ROS), (2) inhibit the activation of nuclear factor kappa B (NF-kappaB), or (3) block the production of interleukin 1 (IL-1) in RAW 264.7 cultured macrophages. The results indicate that FeTMPyP is a potent scavenger of hydroxyl radicals and superoxide anion radicals, and an effective inhibitor of stimulant-induced NF-kappaB activation and IL-1 production by RAW 264.7 cells. Therefore, FeTMPyP may be a useful tool to investigate the molecular mechanisms involved in stimulant-induced signal pathways, and may possess therapeutic utility in diseases associated with overproduction of ROS.
Kang
J L
JL
Department of Physiology, College of Medicine, Ewha Medical Research Center, Ewha Womans University, Seoul, Korea. Jihee@mm.ewha.ac.kr
Lee
H S
HS
Pack
I S
IS
Leonard
S
S
Castranova
V
V
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002470:Cell Survival; D004247:DNA / Q000032:analysis; D024202:Electrophoretic Mobility Shift Assay; D005296:Ferrous Compounds / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008665:Metalloporphyrins / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000096:biosynthesis* / Q000494:pharmacology; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000737:chemistry*; D015398:Signal Transduction
D000975:Antioxidants; D005296:Ferrous Compounds; D007375:Interleukin-1; D008665:Metalloporphyrins; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D004247:DNA