A zebrafish homologue of deleted in colorectal cancer (zdcc) is expressed in the first neuronal clusters of the developing brain.
DCC (deleted in colon cancer), Neogenin and UNC-5 are all members of the immunoglobulin superfamily of transmembrane receptors which are believed to play a role in axon guidance by binding to their ligands, the Netrin/UNC-40 family of secreted molecules (Cell. Mol. Life Sci. 56 (1999) 62; Curr. Opin. Genet. Dev. 7 (1997) 87). Although zebrafish homologues of the Netrin family of secreted molecules have been reported, to date there has been no published description of zebrafish DCC homologues (Mol. Cell. Neurosci. 9 (1997) 293; Mol. Cell. Neurosci. 11 (1998) 194; Mech. Dev. 62 (1997) 147). We report here the expression pattern of a zebrafish dcc (zdcc) homologue during the initial period of neurogenesis and axon tract formation within the developing central nervous system. Between 12 and 33 h post-fertilisation zdcc is expressed in a dynamic spatiotemporal pattern in all major subdivisions of the central nervous system. Double-labelling for zdcc and the post-mitotic neuronal marker HNK-1 revealed that subpopulations of neurons within the first nuclei of the zebrafish brain express zdcc. These results support our previous observation that patterning of neuronal clusters in the zebrafish brain occurs early in development (Dev. Biol. 229 (2001) 271).
Hjorth
J T
JT
Department of Anatomy and Cell Biology, The University of Melbourne, Melbourne, Victoria, 3010, Australia.
Gad
J
J
Cooper
H
H
Key
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D000075389:DCC Receptor; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011956:Receptors, Cell Surface; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development
D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; C501909:DCC protein, human; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins