Prognostic impact of CD31 antigen expression in anal canal carcinoma.
OBJECTIVE
CD31 is a platelet endothelial cell adhesion molecule. Thus CD31 immunostaining of vascular endothelial cells can be used to measure degree of angiogenesis. As angiogenesis is necessary for tumor growth and metastasis, microvessels density could be a predictor of prognosis. The purpose of this study was to examine the relationship between CD31 value and standard pathologic parameters and prognosis of anal canal carcinoma.


METHODS
Twenty-four patients with anal canal carcinoma were evaluated. Five-micron sections of formalin-fixed, paraffin-embedded tissue were tested with monoclonal anti-CD31 antibody. CD31 value is considered positive if more than 185 vessels/mm2 were counted. Pearson's chi 2 test was employed to test for association between CD31 value and clinicopathological variables.


RESULTS
We found no correlation between CD31 value and histologic type, lymph node involvement, patients age and neoplastic relapse. Significant correlation was found between CD31 score and depth of parietal invasion.


CONCLUSIONS
The relapse type could strengthen the hypothesis that increased vascularity promotes neoplastic dissemination. As angiogenesis could be used as prognostic indicator to determine patients who may be at higher risk for relapse, our results warrant further confirmation. Development of markers of angiogenic activity in anal canal carcinoma must be an integral part of proper clinical trials.
Indinnimeo
M
M
Department of Surgery Pietro Valdoni, Università di Roma La Sapienza, via del Policlinico, 155, 00161 Rome, Italy. marileda_indinni@yahoo.it
Cicchini
C
C
Stazi
A
A
Limiti
M R
MR
Ghini
C
C
Mingazzini
P L
PL
Vecchione
A
A
D000328:Adult; D000368:Aged; D001003:Anal Canal / Q000473:pathology; D001005:Anus Neoplasms / Q000473:pathology* / Q000601:surgery; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000601:surgery; D002295:Carcinoma, Transitional Cell / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis*; D011379:Prognosis; D012004:Rectal Neoplasms / Q000473:pathology* / Q000601:surgery; D012007:Rectum / Q000473:pathology
D014408:Biomarkers, Tumor; D019408:Platelet Endothelial Cell Adhesion Molecule-1