Occurrence of neurotrophin receptors and transmitters in the developing Xenopus gut.
The ontogeny of gut innervation in the anuran amphibian Xenopus laevis was studied using immunohistochemistry on sections of whole larvae from NF stages 38-52. Immunoreactivity to acetylated tubulin confirmed the presence of nerve fibres as early as stages 38-39. Actin immunoreactivity was found at stage 41, indicating the presence of smooth muscle cells. Trk-like neurotrophin receptors were occasionally found in nerve fibres as soon as stages 38-39. Vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) immunoreactivities coexisted in nerves innervating the gut wall from stages 40-41, and nitric oxide synthase (NOS) from stage 42. Substance P/neurokinin A (SP/NKA) occurred at stage 42. In all these cases, the first fibres were observed in the oesophagus. Calcitonin gene-related peptide (CGRP) was first observed in nerves at stage 48. In general, VIP/PACAP and NOS innervation was denser than the tachykinin innervation. In conclusion, the development of nerve fibres in the Xenopus gut is probably dependent on neurotrophins that may act via Trk-like receptors and occur before the gut wall is fully organised morphologically. Feeding in Xenopus larvae starts at NF stage 45. The study demonstrates that several of the transmitters investigated are expressed in the gut innervation (and in endocrine cells) prior to this stage.
Holmberg
A
A
Department of Zoophysiology, Göteborg University, Sweden. anna.holmberg@zool.gu.se
Hägg
U
U
Fritsche
R
R
Holmgren
S
S
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism; D017615:Enteric Nervous System / Q000254:growth & development* / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D015320:Tachykinins / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000254:growth & development* / Q000378:metabolism*
C495404:ADCYAP1 protein, Xenopus; D009414:Nerve Growth Factors; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017475:Receptors, Nerve Growth Factor; D015320:Tachykinins; D029867:Xenopus Proteins; D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D015740:Calcitonin Gene-Related Peptide