title: Efficient FLP recombination in mouse ES cells and oocytes.
abstract: We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.
authors.affiliation: Gene Expression Program, EMBL, Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schaft
authors.forename: R
authors.initials: R
authors.lastname: Ashery-Padan
authors.forename: F
authors.initials: F
authors.lastname: van der Hoeven
authors.forename: P
authors.initials: P
authors.lastname: Gruss
authors.forename: A F
authors.initials: AF
authors.lastname: Stewart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D014162:Transfection
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase