[Analyses of cell cycle and DNA].
Cell cycle and DNA index have been analyzed mainly by flow cytometry by staining cells with propidium iodide (PI) and by analyzing obtained data by computer. On the other hand, as bromodeoxyuridine (BrdU) is incorporated into single-stranded DNA, cells labeled by BrdU are in the S phase. Double staining with PI and BrdUrd enabled us to distinguish early S from G0G1 phase and late S from G2M phase. However, recent developments in molecular biology have afforded us evidences that many kinds of proteins, such as products of oncogene and suppressor gene and cell cycle-regulating and suppressing proteins, have important roles in cell cycle progression. In this paper, I would like to introduce our recent results concerning to the cell cycle, especially evaluation of cell cycle by BrdU-staining method, relationship between prognoses of patients with lung cancer after complete resection and cell cycle factors, an association of Ras family with farnesylation inhibitors, effects of microtubules on transportation of MAPK into the nucleus, and localization of Grb2.
Inoue
S
S
Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810.
D048868:Adaptor Proteins, Signal Transducing; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D051380:GRB2 Adaptor Protein; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy; D008870:Microtubules / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011379:Prognosis; D011506:Proteins / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D011506:Proteins; D004247:DNA; D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins; D001973:Bromodeoxyuridine