Renal effects of glibenclamide in cystic fibrosis mice.
In vitro studies have shown that glibenclamide sensitivity is conferred upon Kir 1.1 K(+) channels when they are co-expressed with the cystic fibrosis transmembrane conductance regulator (CFTR). In rats, glibenclamide acts as a K(+)-sparing diuretic by a mechanism that involves blockade of Kir 1.1 channels in the distal nephron. To test whether interaction between Kir 1.1 and CFTR is required to mediate the renal effects of glibenclamide (15 mg/kg), clearance experiments were performed comparing wild type (WT) and Cftr(tm2cam) Delta F508 cystic fibrosis (CF) mice. Glibenclamide treatment was associated with an equivalent diuresis in both WT and CF mice. Glibenclamide was K(+)-sparing in both genotypes with no significant change in urinary K(+) excretion observed. That glibenclamide was an effective K(+)-sparing diuretic in CF animals suggests that CFTR expression is not a requirement to mediate its renal actions in mice.
Kibble
Jonathan
J
Department of Biomedical Science, University of Sheffield, United Kingdom.
Neal
Andrew
A
Department of Biomedical Science, University of Sheffield, United Kingdom.
White
Stanley
S
Department of Biomedical Science, University of Sheffield, United Kingdom.
Green
Roger
R
School of Biological Sciences, University of Manchester, United Kingdom.
Evans
Steve
S
The Sheffield Children's Hospital, Sheffield, United Kingdom.
Taylor
Christopher
C
The Sheffield Children's Hospital, Sheffield, United Kingdom.
D000818:Animals; D003550:Cystic Fibrosis / Q000503:physiopathology* / Q000652:urine; D004232:Diuretics / Q000494:pharmacology*; D005905:Glyburide / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects*; D051379:Mice; D011188:Potassium / Q000652:urine; D012016:Reference Values
D004232:Diuretics; D011188:Potassium; D005905:Glyburide