Molecular cloning and characterization of human PTB-like protein: a possible retinal autoantigen of cancer-associated retinopathy.
Human homologue of polypyrimidine tract binding protein (PTB), a possible autoantigen for cancer-associated retinopathy (CAR), was isolated from a human retinal cDNA library. This homologue, named PTB-like protein (PTBLP), encodes a 532 amino acid residue and has a 75% homology to the human PTB. The human PTBLP had four RNA recognition motifs (RRMs) and had a RNA binding ability. There are four splicing variants in PTBLP. The CAR serum recognized the full length form of PTBLP and the antigenic determinant was localized within 12 amino acids of the C-terminal region. The sequence was included in the fourth RRM sequence.
Tateiwa
H
H
Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto 390-8621, Japan.
Gotoh
N
N
Ichikawa
M
M
Kikuchi
T
T
Yoshimura
N
N
D000595:Amino Acid Sequence / Q000235:genetics; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D020361:Paraneoplastic Syndromes, Nervous System / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D038941:Polypyrimidine Tract-Binding Protein; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000302:isolation & purification; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012160:Retina / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D012164:Retinal Diseases / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
D001324:Autoantigens; D018076:DNA, Complementary; D000939:Epitopes; D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D038941:Polypyrimidine Tract-Binding Protein