Down-regulation and recovery of endothelin-1 binding and signaling in rat cardiomyocytes.
Down-regulation and recovery of endothelin (ET) receptors and of ET-dependent phosphoinositide-specific phospholipase C (PI-PLC) signaling was examined in cultured cardiomyocytes from neonatal rats. Three hours treatment with 5 nM ET-1 decreased surface receptors to 30%, and transduction to 19%, of their respective time-zero values. After extensive washing and a 3 h recovery period surface receptors returned to 74% of the time-zero value, with concomitant recovery of signal transduction to 75% of the time-zero value. The recovery of PI-PLC signaling in these cells is in contrast with a previous report, but consistent with recovery of the receptor complement.
Harvey
S A
SA
Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78284-7760, USA. harveysa@msx.upmc.edu
Gandhi
C R
CR
Behal
R H
RH
Olson
M S
MS
D000818:Animals; D012898:Autacoids / Q000378:metabolism; D001665:Binding Sites; D002478:Cells, Cultured; D015536:Down-Regulation; D019332:Endothelin-1 / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009206:Myocardium / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
D012898:Autacoids; D019332:Endothelin-1; D007457:Iodine Radioisotopes; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase