title: Hemin reconstitutes proton extrusion in an H(+)-ATPase-negative mutant of Lactococcus lactis.
abstract: H(+)-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H(+)-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
authors.affiliation: Department of Chemical Engineering, University of Queensland, 4072 Brisbane, Australia.
authors.forename: L M
authors.initials: LM
authors.lastname: Blank
authors.forename: B J
authors.initials: BJ
authors.lastname: Koebmann
authors.forename: O
authors.initials: O
authors.lastname: Michelsen
authors.forename: L K
authors.initials: LK
authors.lastname: Nielsen
authors.forename: P R
authors.initials: PR
authors.lastname: Jensen
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D006427:Hemin / Q000378:metabolism / Q000502:physiology*; D013294:Lactococcus lactis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
chemical_list: D006427:Hemin; D006180:Proton-Translocating ATPases