Identification of novel G protein-coupled receptor-interacting proteins.
Biochemical and genetic methods utilizing G protein-coupled receptor fragments have been used successfully to identify G protein-coupled receptor-interacting proteins. As noted earlier, these methods may be unable to detect interactions that require certain conformations of the native receptor protein, but have nevertheless proven quite useful in expanding our understanding of receptor regulation to include interactions with proteins other than G proteins, G protein-coupled receptor kinases, and arrestins. Undoubtedly, it is likely that all G protein-coupled receptors have their own unique constellations of associated cytoplasmic proteins, and the techniques described here should prove useful in identifying these.
Premont
Richard T
RT
Liver Center, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA.
Hall
Randy A
RA
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D019204:GTP-Binding Proteins / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D019204:GTP-Binding Proteins