Assisting reproduction of infertile men carrying a Robertsonian translocation.
BACKGROUND
In order to provide better genetic counselling for Robertsonian translocation carriers, the meiotic segregation of chromosomes 13, 14 and 21 from six infertile (13;14) and (14;21) Robertsonian translocation carriers was examined.


METHODS
Dual-colour fluorescence in-situ hybridization analysis using locus-specific probes was carried out on spermatozoa of translocation carriers. Spermatozoa from six proven fertile subjects were analysed using the same probes as controls.


RESULTS
We observed that the frequencies of unbalanced spermatozoa were similar in the (13;14) translocation carriers (9.0, 10.0 and 12.9%) and in the (14;21) translocation carriers (8.7, 7.2 and 7.0%). These frequencies were significantly increased compared with the control population (P < 0.05).


CONCLUSIONS
This high frequency might justify the use of preimplantation genetic diagnosis in these patients where the translocation is usually associated with infertility, requiring intracytoplasmic sperm injection, as it might improve the outcome of the assisted reproduction technique.
Frydman
N
N
Service de Génétique et Reproduction, Hôpital Antoine Béclère, Clamart, France. nelly.frydman@abc.ap-hop-paris.fr
Romana
S
S
Le Lorc'h
M
M
Vekemans
M
M
Frydman
R
R
Tachdjian
G
G
D000328:Adult; D002882:Chromosomes, Human, Pair 13; D002883:Chromosomes, Human, Pair 14; D002891:Chromosomes, Human, Pair 21; D006579:Heterozygote; D006801:Humans; D017403:In Situ Hybridization; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008540:Meiosis; D012099:Reproductive Techniques; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000648:ultrastructure; D014178:Translocation, Genetic