Mass production of pure gibberellin A1 by Phaeosphaeria sp. L487 and the fungal preparation of [U-13C]gibberellin A1.
The mass production of pure gibberellin A1 (GA1) by shake-culturing Phaeosphaeria sp. L487 was investigated. Its GA1 production was markedly influenced by natural nitrogen sources and NH4NO3. When the fungus was cultured in an 8% glucose-1.5% oatmeal-0.1% NH4NO3-0.5% KH2PO4-0.1% MgSO4 x 7H2O medium for 3 weeks, the amount of GA1 in the culture filtrate was up to ca. 200 microg/ml: the addition of safflower oil to the culture medium two weeks after inoculation prolonged the GA1-production period to produce 300 microg/ml. Further preparation of [U-13C]GA, as a tool for the analysis of a complex of GA1 and its binding protein was attempted by using the fungus. The fungal culture in a [U-13C]glucose-oatmeal medium gave 6 mg of crystalline 13C-enriched GA1. Its 13C-enrichment of ca. 75% and 1J(CC) values were determined by NMR spectrometry.
Kenmoku
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The United Graduate School of Agricultural Sciences, Iwate University, Tsuruoka, Japan.
Oozone
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Sugai
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Sassa
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D001203:Ascomycota / Q000378:metabolism*; D002247:Carbon Isotopes / Q000737:chemistry; D003470:Culture Media; D005285:Fermentation; D005875:Gibberellins / Q000096:biosynthesis* / Q000737:chemistry*; D007218:Industrial Microbiology / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy
D002247:Carbon Isotopes; D003470:Culture Media; D005875:Gibberellins; C422660:gibberellin A1