Insulin-like growth factor I measurement on filter paper blood spots.
The measurement of insulin-like growth factor I (IGF-I) from extracted whole dried blood spots on filter paper is a simple method allowing sample collection to be carried out by the patient at home. One drop of blood is applied to a filter card, air dried and posted to the laboratory. The IGF-I is dissociated from its binding protein by an acidification and neutralization procedure, and IGF-I is then measured by an enzyme-linked immunosorbent assay. Results from the analysis of IGF-I using blood spots on filter paper compared favourably with those from conventional methods, thus showing it to be an ideal and cost-effective technique for the measurement of IGF-I in both adults and children.
Jones
J S
JS
Diagnostic Systems Laboratories, Trident Business Centre, Bickersteth Road, London, UK. jjones@DSLabs-uk.com
D001774:Blood Chemical Analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D010209:Paper
D007334:Insulin-Like Growth Factor I