Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: factors regulating the protein release.
A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility.
Balabushevitch
N G
NG
Department of Chemistry, Moscow State University, Vorob'evy Gory, Moscow, 119899, Russia.
Sukhorukov
G B
GB
Moroz
N A
NA
Volodkin
D V
DV
Larionova
N I
NI
Donath
E
E
Mohwald
H
H
D000327:Adsorption; D002214:Capsules / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism
D002214:Capsules; D014361:Trypsin Inhibitors; D002918:Chymotrypsin