[Immunohistochemical study of idiopathic and secondary epiretinal membrane].
OBJECTIVE
We investigated the immunohistochemical features of surgically resected idiopathic epiretinal membranes(ERMs) and secondary ERMs with regard to posterior vitreous detachment(PVD).


METHODS
Six specimens of idiopathic epiretinal membranes(3 eyes with complete PVD, 2 eyes with partial PVD, and one eye with no PVD) and 3 specimens of secondary ERMs(all eyes with complete PVD) were immunohistochemically studied. We used type I, II, III, IV collagen and fibronectin to study extracellular components, and glial fibrillary acidic protein(GFAP), S 100 protein, vimentin, and so forth to study cellular components.


RESULTS
All the specimens of idiopathic ERMs had the major components of the lamellar stained by type II collagen antibody, and one out of 3 specimens of secondary ERMs had a minor component stained by type II collagen antibody. Compared with idiopathic ERMs with complete PVD, 2 out of 3 specimens of idiopathic ERMs with partial PVD or no PVD contained rather thick collagen lamellar.


CONCLUSIONS
There was difference between specimens of idiopathic ERMs and specimens of secondary ERMs in staining by type II collagen antibody, supposed by vitreous, in this study. Idiopathic ERM with attached posterior vitreous membrane may cause growth of collagen.
Kinouchi
R
R
Department of Ophthalmology, Asahikawa Medical College, 2-1 Midorigaoka, Higashi, Asahikawa 078-8510, Japan.
Hirokawa
H
H
Miyokawa
N
N
Nomiyama
T
T
Hikichi
T
T
Yoshida
A
A
D000368:Aged; D003094:Collagen / Q000378:metabolism*; D019773:Epiretinal Membrane / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D014822:Vitreous Body / Q000378:metabolism; D020255:Vitreous Detachment / Q000378:metabolism / Q000473:pathology
D005904:Glial Fibrillary Acidic Protein; D003094:Collagen