title: High-sensitivity negative-ion laser spray for liquid chromatography/mass spectrometry.
abstract: A negative-ion mode laser spray interface for use in liquid chromatography/mass spectrometry (LC/MS) has been developed and investigated. The laser spray gave sensitivities that were orders of magnitude better than the negative-ion electrospray for all samples investigated with the exception of sugars. Fragment ions, HSO(-4)and SO(-3), were formed from the laser-sprayed aqueous solution of cholesterol 3-sulfate sodium salt. This suggests that structural information may be obtained directly from the laser-spray mass spectra for thermally labile compounds.
authors.affiliation: Clean Energy Research Center, Yamanashi University, Takeda-4, Kofu 400-8511, Japan. hiraoka@ab11.yamanashi.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Hiraoka
authors.forename: Y
authors.initials: Y
authors.lastname: Asakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Ueda
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002784:Cholesterol / Q000737:chemistry; D002853:Chromatography, Liquid; D003505:Cyclodextrins / Q000737:chemistry; D007834:Lasers; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013431:Sulfates / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D003505:Cyclodextrins; D013431:Sulfates; D002784:Cholesterol