Preparation of and tissue response to DNA-lipid films.
DNA-containing films have the potential to form complexes with antibiotics or cytokines by intercalation or groove-binding. This principle can be highly relevant for regenerative wound-healing around oral implants and in periodontology. In this study, we prepared DNA-lipid films and examined tissue responses to them as an indicator of their biological properties. The lipids were synthesized from the reaction of L-alanine, n-alkyl alcohol, and p-toluenesulfonic acid. We prepared the self-standing, water-insoluble DNA-lipid films by casting the DNA-lipid complex from chloroform/ethanol solution. The DNA-lipid complexes, which had 1:1 ratios of phosphate anions to cationic lipid, were found to have a double-helical B-form structure. The DNA-lipid films were almost dissolved 3 days after subcutaneous implantation in the backs of rats. There were no inflammatory reactions or inhibition of new tissue formation. We concluded that DNA-lipid films can be prepared by simple methods, and that they do not cause an unfavorable tissue response.
Fukushima
T
T
Department of Dental Materials, Fukuoka Dental College, Japan. tadaof@college.fdcnet.ac.jp
Inoue
Y
Y
Hayakawa
T
T
Taniguchi
K
K
Miyazaki
K
K
Okahata
Y
Y
D020341:Absorbable Implants; D000409:Alanine / Q000737:chemistry; D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D002725:Chloroform / Q000737:chemistry; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D004247:DNA / Q000737:chemistry; D062109:Dermatologic Surgical Procedures; D007851:Dodecanol / Q000737:chemistry; D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D005233:Fatty Alcohols / Q000737:chemistry; D005500:Follow-Up Studies; D006097:Granulation Tissue / Q000473:pathology; D008055:Lipids / Q000737:chemistry; D008297:Male; D008567:Membranes, Artificial; D011487:Protein Conformation; D051381:Rats; D012995:Solubility; D014867:Water; D014945:Wound Healing
D001557:Benzenesulfonates; D001672:Biocompatible Materials; D005233:Fatty Alcohols; D008055:Lipids; D008567:Membranes, Artificial; D014867:Water; D007851:Dodecanol; D000431:Ethanol; D002725:Chloroform; C029383:n-decyl alcohol; D003094:Collagen; D004247:DNA; D000409:Alanine; C029501:4-toluenesulfonic acid