Stress-induced immune changes in the oyster Crassostrea gigas.
Information concerning the effect of stress on invertebrate immune functions are scarce. The present study investigated the consequences of a 15-min mechanical disturbance on immune parameters in oysters Crassostrea gigas. As indicated by noradrenaline and dopamine measurements, the mechanical disturbance caused a transient state of stress in oysters. The number of circulating hemocytes, the migratory and phagocytic activities and reactive oxygen species production of hemocytes were measured before, during and after application of the stressor. Results show that all immune functions were significantly downregulated during stress and a transient period of immunostimulation was observed 30-240 min after the end of the disturbance. Taken together, these results suggest that stress can exert a profound influence on oyster immune functions and they may explain why stress and the outbreak of disease are often linked in shellfish culture. Furthermore, the present study strongly suggests that checking the stress status of animals may be necessary to avoid biases when studying oyster immune responses in vivo.
Lacoste
Arnaud
A
Station Biologique de Roscoff, CNRS-Université Paris VI-INSU, Place Georges Teissier, B.P. 74, F-29682 cedex, Roscoff, France. lacoste@sb-roscoff.fr
Malham
Shelagh K
SK
Gélébart
Florence
F
Cueff
Anne
A
Poulet
Serge A
SA
D000818:Animals; D017756:Aquaculture; D002395:Catecholamines / Q000097:blood; D002465:Cell Movement; D006434:Hemocytes / Q000166:cytology; D006458:Hemolymph / Q000276:immunology; D010125:Ostreidae / Q000276:immunology*; D010587:Phagocytosis; D010812:Physical Stimulation; D013481:Superoxides / Q000032:analysis
D002395:Catecholamines; D013481:Superoxides