Synthesis and characterization of a biospecific adsorbent containing bovine serum albumin as a ligand and its use for bilirubin retention.
Epoxy-activated gels from cross-linked polybutadiene-hydroxyethylmethacrylate (PB-HEMA) copolymer and epichlorohydrin (ECH) were prepared and characterized. Albumin was covalently bonded to the matrix and used as support of affinity chromatography in bilirubin (BR) retention experiments. PB-HEMA-ECH with different amounts of immobilized albumin (between 5.20 and 6.80 mg/g dry gel) were obtained. Bilirubin retention of 3.10 mg/g of these beads was observed at 5 degrees C.
Alvarez
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Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Edificio de Ciencas II, Ciudad Universitaria, Córdoba (5000), Argentina.
Strumia
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Bertorello
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D000327:Adsorption; D000818:Animals; D001663:Bilirubin / Q000302:isolation & purification*; D002070:Butadienes; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D020724:Elastomers; D008024:Ligands; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine
D002070:Butadienes; D020724:Elastomers; D008024:Ligands; D008689:Methacrylates; D011108:Polymers; D012710:Serum Albumin, Bovine; C005044:hydroxyethyl methacrylate; C028834:polybutadiene; D001663:Bilirubin