title: Electrophysiological and morphological alterations in peripheral nerves by the pig paramyxovirus of blue eye disease in neonatal pigs.
abstract: The pig paramyxovirus of blue eye disease (PPBED) produces central nervous system (CNS) damage leading to death in piglets. However, when PPBED was injected into the muscle and came into contact with hind limb peripheral nerves and was transported to the CNS, it did not cause death and could be a mechanism by which to induce protection. This study analyses whether PPBED causes electrophysiological and morphological alterations in infected hind limb peripheral nerves. It also studies, whether PPBED induces the onset of haemagglutination inhibitory antibodies (HIA) when it is transported to the spinal cord after medial gastrocnemius (MG) intramuscular injection. PPBED was detected by an immunohistochemical method and nerve morphology was studied using electron microscopy. The physiological status of the nerve was evaluated with electrophysiological techniques. The electrical threshold of the infected MG nerve increased four- or five fold compared to that in the ipsilateral lateral gastrocnemius or in the MG nerve on the control side. The infected nerve fibres underwent myelin sheet disarrangement and their internal fibre diameter decreased. PPBED induced the onset of HIA.
authors.affiliation: Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada, México.
authors.forename: M A
authors.initials: MA
authors.lastname: Ramírez-Herrera
authors.forename: M L
authors.initials: ML
authors.lastname: Mendoza-Magaña
authors.forename: J M
authors.initials: JM
authors.lastname: Dueñas-Jiménez
authors.forename: J
authors.initials: J
authors.lastname: Mora-Galindo
authors.forename: S H
authors.initials: SH
authors.lastname: Dueñas-Jiménez
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D004594:Electrophysiology; D006614:Hindlimb; D007273:Injections, Intramuscular; D018482:Muscle, Skeletal / Q000294:innervation*; D010525:Peripheral Nerves / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D018108:Respirovirus / Q000276:immunology / Q000472:pathogenicity*; D010253:Respirovirus Infections / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000503:physiopathology / Q000821:virology*
chemical_list: D000914:Antibodies, Viral