Action mechanism of betahistine in the vestibular end organs.
Betahistine has been used to treat several vestibular disorders of both central and peripheral origin. The objective of this work was to study the betahistine action mechanism at the vestibular end organs. Experiments were carried out in wild larval axolotl (Ambystoma tigrinum). Multiunit extracellular recordings were obtained from the semicircular canal nerve using a suction electrode. Betahistine (10 microM to 10 mM, n = 32) inhibited the basal spike discharge of the vestibular afferent neurons with an IC50 of 600 microM. To define the site of action of betahistine, its interactions with antagonists of nitric oxide sintethizing enzyme, cholinergic drugs, and excitatory amino acids were studied. Betahistine 1 mM (n = 5) was coadministered with NG-nitro-L-arginine 3 microM. The action of betahistine remained as in control experiments. Betahistine 1 mM reduced the excitatory action of carbachol (200 microM, n = 5) in a 30 +/- 3.4%. Cholinergic antagonists atropine (10 microM, n = 3) and d-tubocurarine (10 microM, n = 3) did not modify betahistine actions. Betahistine 1 mM also reduced kainic acid (10 microM, n = 4) excitatory action in 45.5 +/- 9.8%. These results corroborate that betahistine has a peripheral inhibitory action in the spike discharge of the afferent neurons in the vestibule. This action seems to involve neither NO production nor modifications in the release of acetylcholine from the efferent fibers. The inhibitory action of betahistine seems to be due to a postsynaptic binding site on the afferent neurons.
Chávez
H
H
Instituto de Fisiología, Universidad Autónoma de Puebla, Facultad de Estomatología, Universidad Autónoma de Puebla, Puebla, México.
Vega
R
R
Valli
P
P
Mira
E
E
Benvenuti
C
C
Guth
P S
PS
Soto
E
E
D000557:Ambystoma; D000818:Animals; D001621:Betahistine / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology*; D014722:Vestibule, Labyrinth / Q000187:drug effects* / Q000502:physiology
D014665:Vasodilator Agents; D001621:Betahistine