Long-term tumor growth suppression in mice immunized with naked DNA of the human tumor antigen mucin (MUC1).
Naked DNA is an attractive tool for vaccination studies. We have studied naked DNA vaccination against the human tumor antigen mucin, encoded by the gene MUC1. C57/BL6 mice were immunized twice, on day 1 and day 10. with plasmid pCI-MUC1, intramuscularly. Five days after the last immunization tumor challenge experiments were performed using the tumor cell line MC38, expressing human MUC1. In 85% of mice immunized with the mucin plasmid tumor growth inhibition was observed, whereas control mice developed tumors. Re-tumor challenge after three months revealed no tumor growth in mice immunized with the mucin plasmid. These encouraging results, showing long-term protection against tumor growth, indicate the potential usefulness of naked DNA vaccination for clinical immunotherapy.
Johnen
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Humboldt-University Berlin, Charité Campus Mitte, AG Molecular Gene- and Immunotherapy, Berlin, Germany.
Kulbe
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Pecher
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D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000032:analysis / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D014162:Transfection; D014407:Tumor Cells, Cultured; D019444:Vaccines, DNA / Q000276:immunology*
D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D018396:Mucin-1; D019444:Vaccines, DNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor