title: Identification of tumor-infiltrating macrophages as the killers of tumor cells after immunization in a rat model system.
abstract: Immunization can prevent tumor growth, but the effector cells directly responsible for tumor cell killing in immunized hosts remain undetermined. The present study compares tumor grafts that progress in naive syngeneic rats with the same grafts that completely regress in hosts preimmunized with an immunogenic cell variant. The progressive tumors contain only a few macrophages that remain at the periphery of the tumor without direct contact with the cancer cells. These macrophages do not kill tumor cells in vitro. In contrast, tumors grafted in immunized hosts and examined at the beginning of tumor regression show a dramatic infiltration with mature macrophages, many of them in direct contact with the cancer cells. These macrophages are strongly cytotoxic for the tumor cells in vitro. In contrast to macrophages, tumor-associated lymphocytes are not directly cytotoxic to the tumor cells, even when obtained from tumor-immune rats. However, CD4(+) and CD8(+) T cells prepared from the regressing tumors induce tumoricidal activity in splenic macrophages from normal or tumor-bearing rats and in macrophages that infiltrate progressive tumors. These results strongly suggest that the main tumoricidal effector cells in preimmunized rats are macrophages that have been activated by adjacent tumor-immune lymphocytes.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 517, Institut Federatif de Recherche 100, Faculty of Medicine, Dijon, France.
authors.forename: B
authors.initials: B
authors.lastname: Bonnotte
authors.forename: N
authors.initials: N
authors.lastname: Larmonier
authors.forename: N
authors.initials: N
authors.lastname: Favre
authors.forename: A
authors.initials: A
authors.lastname: Fromentin
authors.forename: M
authors.initials: M
authors.lastname: Moutet
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: S
authors.initials: S
authors.lastname: Gurbuxani
authors.forename: E
authors.initials: E
authors.lastname: Solary
authors.forename: B
authors.initials: B
authors.lastname: Chauffert
authors.forename: F
authors.initials: F
authors.lastname: Martin
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D005260:Female; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy*; D051381:Rats
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D008070:Lipopolysaccharides; D007371:Interferon-gamma