Analysis of transition from long-term nonprogressive to progressive infection identifies sequences that may attenuate HIV type 1.
Long-term nonprogressive human immunodeficiency virus type 1 (HIV-1) infection and its transition to progressive infection presents an opportunity to identify the molecular determinants of HIV-1 attenuation and pathogenesis. We studied an individual who underwent a transition from long-term nonprogressive to rapidly progressive infection. Because HIV-1 RNA genomes in plasma represent replicating virus, we developed a technique to clone full-length HIV-1 RNA genomes from plasma and used this technique to obtain clones from this individual before and during the transition. Most clones assayed were infectious, demonstrating that the RNA genomes encoded viable virus. Analysis of 20 complete HIV-1 RNA genomic sequences revealed one major difference between sequences found during the two phases of infection. During the nonprogressive phase, the predominant sequences had a large deletion in an Sp1-binding site and adjacent promoter in the U3 part of the long terminal repeat (LTR); when the infection became progressive, all viruses had intact Sp1 and promoter sequences and were derived from a minor species present earlier. Analysis of 184 clones of the LTR region obtained at five time points spanning a 7-year period confirmed this switch. In an in vitro assay, the deletion downregulated LTR-driven transcription of a reporter gene. In addition, analysis of cytotoxic T lymphocyte (CTL) epitopes predicted from the complete viral RNA genomes revealed multiple potential escape mutants that accumulated by the time of progression. These studies suggest that during the nonprogressive phase, the Sp1 enhancer-promoter deletion is likely to have played a role in decreasing replication, thereby attenuating HIV-1. The accumulation of CTL escape mutants suggests that a breakdown in immunologic surveillance may have allowed proliferation of intact virus, thus leading to rapid disease progression. These data reveal the viral and immune interactions characterizing a transition from long-term nonprogressive to rapidly progressive infection.
Fang
G
G
Wadsworth Center, New York State Department of Health, Albany, New York 12201, USA.
Burger
H
H
Chappey
C
C
Rowland-Jones
S
S
Visosky
A
A
Chen
C H
CH
Moran
T
T
Townsend
L
L
Murray
M
M
Weiser
B
B
D001483:Base Sequence; D001665:Binding Sites; D004279:DNA, Viral; D018450:Disease Progression; D018984:Epitopes, T-Lymphocyte / Q000032:analysis / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D016325:HIV Long Terminal Repeat; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000032:analysis*; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D004279:DNA, Viral; D018984:Epitopes, T-Lymphocyte; D012367:RNA, Viral; D016329:Sp1 Transcription Factor