title: Biocatalytic chlorination of aromatic hydrocarbons by chloroperoxidase of Caldariomyces fumago.
abstract: Chloroperoxidase from Caldariomyces fumago was able to chlorinate 17 of 20 aromatic hydrocarbons assayed in the presence of hydrogen peroxide and chloride ions. Reaction rates varied from 0.6 min(-1) for naphthalene to 758 min(-1) for 9-methylanthracene. Mono-, di- and tri-chlorinated compounds were obtained from the chloroperoxidase-mediated reaction on aromatic compounds. Dichloroacenaphthene, trichloroacenaphthene, 9,10-dichloroanthracene, chloropyrene, dichloropyrene, dichlorobiphenylene and trichlorobiphenylene were identified by mass spectral analyses as products from acenaphthene, anthracene, pyrene and biophenylene respectively. Polycyclic aromatic hydrocarbons with 5 and 6 aromatic rings were also substrates for the chloroperoxidase reaction. The importance of the microbial chlorination of aromatic pollutants and its potential environmental impact are discussed.
authors.affiliation: Biotechnology Institute UNAM, AP 510-3, Cuernavaca, 62271, Morelos, Mexico. vazqduh@ibt.unam.mx
authors.forename: R
authors.initials: R
authors.lastname: Vázquez-Duhalt
authors.forename: M
authors.initials: M
authors.lastname: Ayala
authors.forename: F J
authors.initials: FJ
authors.lastname: Márquez-Rocha
mesh_terms: D002384:Catalysis; D002711:Chloride Peroxidase / Q000378:metabolism*; D005658:Fungi / Q000201:enzymology*; D006843:Hydrocarbons, Chlorinated / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics
chemical_list: D006843:Hydrocarbons, Chlorinated; D002711:Chloride Peroxidase