Flow injection analysis with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS).
Studies on the capabilities of flow injection analysis (FIA) combined with on-line characterisation of model compounds via a combination of diode array UV, 1H-NMR, FT-IR spectroscopy and mass spectrometry are described. Using this combination of spectrometers enabled the on-flow collection of UV, 1H-NMR, IR and MS for a range of model compounds. Samples were introduced into the system as solutions in deuterium oxide in concentrations ranging from 1.4 to 8.4 mg ml(-1). A sample volume of 100 microl was used for FIA at a flow rate of 1 ml min(-1). From these studies a practical working quantity of ca. 140 microg/sample of analyte was determined which provided characteristic spectra.
Lenz
Eva
E
AstraZeneca Pharmaceuticals, Drug Metabolism & Pharmacokinetics Department, Mereside, Alderley Park, Macclesfield, SK10 4TG, Cheshire, UK.
Taylor
Steve
S
Collins
Craig
C
Wilson
Ian D
ID
Louden
Dave
D
Handley
Alan
A
D010129:4-Aminobenzoic Acid / Q000032:analysis; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
D010129:4-Aminobenzoic Acid