Oximidines I and II: Novel Antitumor Macrolides from Pseudomonas sp.
Our screening for cell-cycle inhibitors in transformed cells resulted in the isolation of novel active compounds, oximidines I (1) and II (2), from Pseudomonas sp. Q52002. The molecular formulas of 1 and 2 were established as C(23)H(24)N(2)O(7) and C(23)H(24)N(2)O(6), respectively, by high-resolution FABMS. The structures of the oximidines were elucidated by NMR spectral analysis, including a variety of two-dimensional techniques. The absolute stereochemistry of 1 was determined by using the modified Mosher method. The oximidines are novel 12-membered macrolides containing an O-methyloxime moiety. Oximidines I and II selectively inhibited the growth of rat 3Y1 cells transformed with E1A, ras, or src oncogenes.
Kim
Jin Woo
JW
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan, and Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.
Shin-Ya
Kazuo
K
Furihata
Kazuo
K
Hayakawa
Yoichi
Y
Seto
Haruo
H