title: Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis.
abstract: We have isolated 14 differentially displayed and 10 further expressed sequence tags (ESTs) from Musculus biceps femoris of newborn healthy and splay leg piglets. By comparison with EMBL/GenBank data we could identify nine porcine homologues to human genes (TATA box binding protein associated factor B TAF1B; B-cell CLL/lymphoma 7B BCL7B; pyruvate dehydrogenase kinase, isoenzyme 4 PDK4; ribosomal protein S10 RPS10; SPARC-like 1 SPARCL1; epithelial protein lost in neoplasm beta EPLIN; N-myc downstream-regulated gene 2 NDRG2; pleiomorphic adenoma gene like 2 PLAGL and, BCL-2 associated transcription factor short form BTFS). Eight fragments correspond to uncharacterized ESTs and 7 ESTs had no significant match with database sequences. These data provide the first expression profiles in skeletal muscle of neonatal piglets and are a basis for candidate gene investigations for congenital splay leg in piglets. Eleven ESTs were physically mapped to porcine chromosomes 1, 3, 4, 5, 7, 8, 9 and 10 and contribute to the comparative map of humans and pigs.
authors.affiliation: Institute of Animal Breeding and Husbandry with Veterinary Clinic, Martin-Luther-University Halle-Wittenberg, Halle, Germany. maak@landw.uni-halle.de
authors.forename: S
authors.initials: S
authors.lastname: Maak
authors.forename: S
authors.initials: S
authors.lastname: Jaesert
authors.forename: K
authors.initials: K
authors.lastname: Neumann
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: G
authors.initials: G
authors.lastname: von Lengerken
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002874:Chromosome Mapping / Q000662:veterinary; D020224:Expressed Sequence Tags; D006614:Hindlimb / Q000002:abnormalities; D006801:Humans; D006822:Hybrid Cells; D018482:Muscle, Skeletal / Q000378:metabolism*; D009135:Muscular Diseases / Q000235:genetics / Q000662:veterinary*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000235:genetics*