Visualizing antibody-catalyzed retro-aldol-retro-Michael reactions.
We developed a visible detection system for antibody-catalyzed retro-aldol-retro-Michael reactions. Aldolase antibody 38C2 catalyzed the reaction of substrate 1 to provide 6-bromo-2-napthol that forms a visible colored azo dye with diazonium salts. This system has potential for the screening of novel catalysts.
Tanaka
F
F
Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Kerwin
L
L
Kubitz
D
D
Lerner
R A
RA
Barbas
C F
CF
D000818:Animals; D017151:Antibodies, Catalytic / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D001711:Biotransformation; D002384:Catalysis; D003979:Diazonium Compounds; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D006825:Hybridomas / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D009284:Naphthols / Q000737:chemistry
D017151:Antibodies, Catalytic; D003979:Diazonium Compounds; D009284:Naphthols; C034667:6-bromo-2-naphthol; D005634:Fructose-Bisphosphate Aldolase