title: Age-related effects on atherogenesis and scavenger enzymes of intracranial and extracranial arteries in men without classic risk factors for atherosclerosis.
abstract: OBJECTIVE
  Atherosclerosis occurs later and is less extensive in intracranial arteries than in extracranial arteries. However, the mechanisms responsible are poorly understood. A previous study has suggested a better antioxidant protection of intracranial arteries.
  
  
  METHODS
  To assess the influence of age on arterial activity of antioxidant enzymes and atherogenesis, we compared intracranial and extracranial arteries of humans of different ages who retrospectively lacked confounding classic risk factors (48 premature fetuses aged 6.4+/-0.8 months [mean+/-SD], 58 children aged 7.9+/-3.8 years, 42 adults aged 42.5+/-5.1 years, and 40 elderly subjects aged 71.8+/-3.4 years; all males). Lesions were quantified by computer-assisted imaging analysis of sections of the middle cerebral and basilar arteries, the left anterior descending coronary artery, the common carotid artery, and the abdominal aorta. Macrophages, apolipoprotein B, oxidized LDL, and matrix metalloproteinase-9 in lesions were determined by immunocytochemistry. The effect of aging on atherogenesis was then compared with that on the activity of 4 antioxidant enzymes in the arterial wall.
  
  
  RESULTS
  Atherosclerosis was 6- to 19-fold greater (P<0.01) in extracranial arteries than in intracranial arteries, and it increased linearly with age. Intracranial arteries showed significantly greater antioxidant enzyme activities than did extracranial arteries. However, the antioxidant protection of intracranial arteries decreased significantly in older age, coinciding with a marked acceleration of atherogenesis. An increase in matrix metalloproteinase-9 protein expression and in gelatinolytic activity consistent with the degree of intracranial atherosclerosis was also observed.
  
  
  CONCLUSIONS
  These results suggest that a greater activity of antioxidant enzymes in intracranial arteries may contribute to their greater resistance to atherogenesis and that with increasing age intracranial arteries respond with accelerated atherogenesis when their antioxidant protection decreases relatively more than that of extracranial arteries.
authors.affiliation: Departments of Medicine, Federico II University of Naples, Naples, Italy. Department of Vascular Surgery, Rigshospitalet, Copenhagen, Denmark.
authors.forename: F P
authors.initials: FP
authors.lastname: D'Armiento
authors.forename: A
authors.initials: A
authors.lastname: Bianchi
authors.forename: F
authors.initials: F
authors.lastname: de Nigris
authors.forename: D M
authors.initials: DM
authors.lastname: Capuzzi
authors.forename: M R
authors.initials: MR
authors.lastname: D'Armiento
authors.forename: G
authors.initials: G
authors.lastname: Crimi
authors.forename: P
authors.initials: P
authors.lastname: Abete
authors.forename: W
authors.initials: W
authors.lastname: Palinski
authors.forename: M
authors.initials: M
authors.lastname: Condorelli
authors.forename: C
authors.initials: C
authors.lastname: Napoli
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000975:Antioxidants / Q000032:analysis; D001055:Apolipoproteins B / Q000032:analysis / Q000276:immunology; D001158:Arteries / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D002536:Cerebral Arteries / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology; D002648:Child; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D002537:Intracranial Arteriosclerosis / Q000201:enzymology* / Q000209:etiology* / Q000473:pathology; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000032:analysis / Q000276:immunology; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D012189:Retrospective Studies; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000032:analysis / Q000276:immunology
chemical_list: D000975:Antioxidants; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D013482:Superoxide Dismutase; D020780:Matrix Metalloproteinase 9