Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
Collins
E
E
The National Diagnostics Centre, BioResearch Ireland, National University of Ireland. Evelyn.Collins@nuigalway.ie
Glennon
M
M
Hanley
S
S
Murray
A M
AM
Cormican
M
M
Smith
T
T
Maher
M
M
D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology*; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003124:Colorimetry; D015342:DNA Probes / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008567:Membranes, Artificial; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D015342:DNA Probes; D008567:Membranes, Artificial; D011933:Reagent Kits, Diagnostic