Synchronized cyclic capillary electrophoresis using channels arranged in a triangle and low voltages.
Synchronized cyclic capillary electrophoresis (SCCE) makes use of a closed loop separation channel by which the same sample can be separated during many cycles. This enables the repeated use of the same voltage for separations such that a high total voltage, and thus high efficiency, is obtained for the synchronized components. This can be accomplished by using any type of polygon geometry for the separation channel; and calculations of the available field and number of connections needed for polygons from 3 to 5 sides are presented. Triangular designs have the advantage of using the lowest number of wells. Such designs are described, with two additional features compared to that of earlier work: 1. voltage connections that are much shallower than the separation channel, to reduce losses and dispersion at the intersections; and 2. corners that are narrower than the separation channels to reduce dispersion in the turns. Experimental data is presented for the separation of a mixture of amino acids, and for a DNA separation in a polymeric sieving matrix. The DNA separation is most sensitive to the corner dispersion problem, which reduces the observed efficiency for that separation.
Manz
A
A
AstraZeneca/SmithKline Beecham Centre for Analytical Sciences, Dept Chemistry, Imperial College, London, UK. a.manz@ic.ac.uk
Bousse
L
L
Chow
A
A
Metha
T B
TB
Kopf-Sill
A
A
Parce
J W
JW
D000596:Amino Acids / Q000302:isolation & purification*; D004247:DNA / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D004867:Equipment Design; D008832:Microchemistry / Q000295:instrumentation* / Q000379:methods
D000596:Amino Acids; D004247:DNA