An enhancer sequence directs LacZ expression to developing pharyngeal endoderm in transgenic mice.
The murine Hoxc-6 homeobox gene comprises three exons with two distinct promoters (PRI and PRII) located 9 kb apart. To characterise the PRII promoter, a region 3 kb upstream of the transcription start site was sequenced, and an Antananapedia-like consensus binding sequence was found (Coletta et al., 1991). A LacZ reporter gene construct, containing three copies of this sequence, directs highly specific expression in cells forming pharyngeal endoderm in transgenic mice. Expression was first detected in a few individual anterior endoderm cells at E7.5, which increase in number up to E9.5, where expression was clearly visible in the pharyngeal endoderm. Expression of the endodermal genes HNF3beta, Pax-9, Shh, and Nkx2.5 showed colocalization with the LacZ-positive cells in the foregut and pharyngeal endoderm. This novel enhancer provides a means of tracking the morphogenetic movement of endodermal cells fated to form the foregut.
Parmar
H
H
Coletta
P L
PL
Faruque
N
N
Sharpe
P T
PT
D000818:Animals; D001483:Base Sequence; D004707:Endoderm / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D016612:Paraffin Embedding; D010614:Pharynx / Q000196:embryology* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
D012333:RNA, Messenger; D001616:beta-Galactosidase