The antioxidant effect of free bilirubin on cumene-hydroperoxide treated human leukocytes.
To examine the antioxidant effect of bilirubin (BR) on leukocyte, we treated leukocytes obtained from healthy subjects with an oxidant and various concentrations of BR. High concentrations of BR decreased thiobarbituric acid reactive substances (TBARS) and catalase activities, increased superoxide dismutase (SOD) activity, but had no effect on glutathione (GSH) concentration. Our results showed that under physiological conditions, BR has an antioxidant effect only in high concentrations.
Yesilkaya
A
A
Department of Biochemistry, School of Medicine, Akdeniz University, 07070 Antalya, Turkey. yesilka@med.akdeniz.edu
Altinayak
R
R
Korgun
D K
DK
D000975:Antioxidants / Q000008:administration & dosage*; D001555:Benzene Derivatives / Q000494:pharmacology*; D001663:Bilirubin / Q000008:administration & dosage*; D002374:Catalase / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005978:Glutathione / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000201:enzymology; D008163:Luminescent Measurements; D008297:Male; D016877:Oxidants / Q000494:pharmacology*; D012016:Reference Values; D013482:Superoxide Dismutase / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D000975:Antioxidants; D001555:Benzene Derivatives; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C007164:cumene hydroperoxide; D001663:Bilirubin