Resolution of Escherichia coli O157:H7 that contaminated radish sprouts in two outbreaks by two-dimensional gel electrophoresis.
Two-dimensional gel electrophoresis (2-DE) was performed to examine exoproteins and periplasmic proteins of Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains isolated from cases associated with radish sprouts in two outbreaks. We found that STEC O157:H7 released a large number of proteins into the medium during the stationary phase of growth, as observed with 2-DE. Although pulsed-field gel electrophoresis (PFGE) patterns of STECs NGY9 (RIMD0509894), a Sakai isolate; NGY33, a Gamagoori isolate; and NGY120, a Kanagawa isolate, were all the same, comparison of 2-DE patterns of exoproteins and periplasmic proteins clarified that NGY9 was distinct from NGY33, whereas NGY33 and NGY120 were of close lineage. We therefore suggest that 2-DE analysis of exoproteins and periplasmic proteins is a powerful epidemiological method with high resolution.
Yokoyama
K
K
Department of Molecular Bacteriology, Nagoya University Postgraduate School of Medicine, Aichi, Japan.
Iinuma
Y
Y
Kawano
Y
Y
Nakano
M
M
Kawagishi
M
M
Yamashino
T
T
Hasegawa
T
T
Ohta
M
M
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D003470:Culture Media; D004196:Disease Outbreaks; D015180:Electrophoresis, Gel, Two-Dimensional; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000737:chemistry* / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D014675:Vegetables / Q000382:microbiology*
D001426:Bacterial Proteins; D003470:Culture Media