A non-natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe.
A small and highly fluorescent non-natural amino acid that contains an anthraniloyl group (atnDap) was incorporated into various positions of streptavidin. The positions were directed by a CGGG/CCCG four-base codon/anticodon pair. The non-natural mutants were obtained in excellent yields and some of them retained strong biotin-binding activity. The fluorescence wavelength as well as the intensity of the anthraniloyl group at position 120 were sensitive to biotin binding. These unique properties indicate that the atnDap is the most suitable non-natural amino acid for a position-specific fluorescent labeling of proteins that is highly sensitive to microenvironmental changes.
Taki
M
M
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Okayama, Japan.
Hohsaka
T
T
Murakami
H
H
Taira
K
K
Sisido
M
M
D000596:Amino Acids / Q000138:chemical synthesis* / Q000737:chemistry*; D001665:Binding Sites / Q000235:genetics; D001710:Biotin; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D062367:ortho-Aminobenzoates / Q000138:chemical synthesis* / Q000737:chemistry*
D000596:Amino Acids; D005456:Fluorescent Dyes; D011506:Proteins; D011994:Recombinant Proteins; C439761:beta-anthraniloyl-amino-beta-diaminopropanoic acid; D062367:ortho-Aminobenzoates; D001710:Biotin; D019809:Streptavidin