title: Human TSC-22 gene: no association with type 2 diabetes.
abstract: OBJECTIVE
  In order to elucidate whether or not genetic variations of TSC-22 (TGF [transforming growth factor]-beta-stimulated clone 22), which was originally identified as a TGF-beta-responsive leucine zipper protein in murine osteoblastic cells, are associated with type 2 diabetes, the genomic organization of the human TSC-22 gene was determined and the association between its polymorphisms and type 2 diabetes was examined.
  
  
  RESULTS
  The human TSC-22 gene spans approximately 5 kilobase pairs and is encoded in three exons. Two single nucleotide polymorphisms (SNPs) were identified in the coding region of the first exon, two other SNPs in the first intron, and one SNP in the putative promoter region. There were, however, no significant differences in the frequency of these polymorphisms between patients with type 2 diabetes and non-diabetic control subjects.
  
  
  CONCLUSIONS
  It is unlikely that the TSC-22 gene is a locus responsible for type 2 diabetes.
authors.affiliation: Department of Metabolism and Clinical Nutrition, Graduate School of Medicine, Kyoto University.
authors.forename: F
authors.initials: F
authors.lastname: Sugawara
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: A
authors.initials: A
authors.lastname: Kuroe
authors.forename: Y
authors.initials: Y
authors.lastname: Someya
authors.forename: A
authors.initials: A
authors.lastname: Kubota
authors.forename: Y
authors.initials: Y
authors.lastname: Ihara
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
mesh_terms: D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D000078790:Insulin Secretion; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012097:Repressor Proteins / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D007328:Insulin; D012097:Repressor Proteins; C099726:TSC22D1 protein, human