Difference in the way of macrophage recognition of target cells depending on their apoptotic states.
Dying cells are selectively eliminated from the organism by phagocytosis. Previous studies suggested the existence of some other phagocytosis marker(s) that function together with phosphatidylserine, the best-characterized phagocytosis marker. We obtained here a monoclonal antibody named PH2 that inhibited macrophage phagocytosis of late apoptotic or necrotic cells, but not of early apoptotic cells. On the other hand, phagocytosis of cells at any time during the process of apoptosis was inhibitable by phosphatidylserine-containing liposomes. Inhibition occurred even when target cells were preincubated with PH2 and separated from unbound antibodies. Moreover, PH2 bound to apoptotic cells at late stages more efficiently than to those at early stages, and it did not bind to normal cells unless their plasma membrane was permeabilized. These results suggest that the putative PH2 antigen is a novel phagocytosis marker that translocates to the cell surface at late stages of apoptosis, resulting in maximal recognition and engulfment by macrophages.
Fujii
C
C
Graduate School of Natural Science and Technology, Kanazawa University, Takara-machi, Kanazawa, Ishikawa 920-0934, Japan.
Shiratsuchi
A
A
Manaka
J
J
Yonehara
S
S
Nakanishi
Y
Y
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000954:Antigens, Surface / Q000276:immunology; D017209:Apoptosis / Q000502:physiology*; D015415:Biomarkers; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D010718:Phosphatidylserines / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015415:Biomarkers; D010718:Phosphatidylserines