title: Immortal and mortal clonal lymphocyte lines from channel catfish: comparison of telomere length, telomerase activity, tumor suppressor and heat shock protein expression.
abstract: Channel catfish autonomous (immortal) and nonautonomous (mortal) leukocyte lines were phenotyped with respect to telomere length and the expression of telomerase, Hsp70 and p53, potentially important factors in cellular immortalization. The autonomous cells constitutively expressed telomerase whereas the nonautonomous cells expressed this activity only transiently. This observation, coupled with the low telomerase activity level seen in freshly isolated leukocytes, suggests that telomerase expression in catfish leukocytes is activation induced. In contrast both types of cell lines exhibited quite similar patterns of significantly shortened telomeres, suggesting that telomerase does not stabilize catfish telomeres until a critical short length is reached. Northern analyses indicated that, like telomerase, Hsp70 gene expression was constitutive in autonomous cells and transient in nonautonomous cells. In contrast, p53 mRNA levels appeared similarly low and noncycling in both long-term cultured types of catfish cells, regardless of the culture situation. Furthermore it was noted, by Western analyses, that both types of cells display multiple sized forms of p53 proteins. This latter observation implies that truncation of p53 protein is probably not directly involved in the in vitro immortalization process of channel catfish leukocytes.
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Barker
authors.forename: Morad
authors.initials: M
authors.lastname: Khayat
authors.forename: Norman
authors.initials: N
authors.lastname: Miller
authors.forename: Melanie
authors.initials: M
authors.lastname: Wilson
authors.forename: L William
authors.initials: LW
authors.lastname: Clem
authors.forename: Eva
authors.initials: E
authors.lastname: Bengtén
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D007059:Ictaluridae / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology; D019098:Telomerase / Q000032:analysis; D016615:Telomere / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D018840:HSP70 Heat-Shock Proteins; D016159:Tumor Suppressor Protein p53; D019098:Telomerase