title: Effect and mechanism of electronic magnetic pulse on peripheral lymphocytes in dogs.
abstract: OBJECTIVE
  To study the effects of electronic magnetic pulse (EMP) on peripheral lymphocytes in dogs and to explore the mechanisms of the biological effects of EMP.
  
  
  METHODS
  T, TH and TS lymphocytes were estimated by acid phosphatase cytochemistry. Apoptotic lymphocytes and Bax and Bcl-2 proteins related to apoptosis were observed with in situ terminal labeling and immunocytochemistry.
  
  
  RESULTS
  Peripheral T lymphocyte subpopulations decreased obviously after EMP irradiation with (2-12) x 10(4) V/m. Apoptotic percentages of lymphocytes increased with the elevation of EMP doses. Ten days after different intensity radiation, the Bax protein was found to be elevated in accord with the peak value of lymphocyte apoptosis. However, Bcl-2 protein decreased obviously.
  
  
  CONCLUSIONS
  A definite field intensity EMP could induce injury to lymphocytes. Apoptosis induced by EMP is one of the main causes of peripheral lymphocyte death and leads to immunosuppression of the body. These results suggest that people should pay more attention to the injury caused by EMP, especially to the immunological functions of the body.
authors.affiliation: Department of Experimental Pathology, Institute of Radiation Medicine, Beijing 100850, China. gaoyb@nic.bmi.ac.cn
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: X
authors.initials: X
authors.lastname: Cui
authors.forename: C
authors.initials: C
authors.lastname: Xiong
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: D
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D004285:Dogs; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008280:Magnetics; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology / Q000528:radiation effects*; D051028:bcl-2-Associated X Protein
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein