title: The isoprostane 8-epi-PGF(2alpha) is a valuable indicator of oxidative injury in human heart valves.
abstract: To date, little information is available concerning oxidative injury in human cardiac valves. Therefore, we sought to investigate whether the isoprostane, 8-epi-PGF(2alpha), a novel oxidative stress marker, is localized in aortic and pulmonary valves derived from explanted hearts of patients suffering from idiopathic dilative cardiomyopathy (IDC). By using semiquantitative immunohistochemistry, we demonstrated that 8-epi-PGF(2alpha) is localized in both valves with pulmonary valves accumulating more of this isoprostane compared to aortic valves (36.69+/-12.04% vs. 31.54+/-11.49%, P<.05). These results were confirmed by a radioimmunoassay (RIA) analysis showing a similar, but not significant, difference between the two valves (288.50+/-72.18 pg/mg protein in the pulmonary valves and 267.30+/-58.77 pg/mg protein in aortic valves, P=.09). Considering the data presented in this study, we suggest that 8-epi-PGF(2alpha) is a valuable indicator of oxidative injury in human semilunar valves.
authors.affiliation: Department of Cardiology, University of Vienna, Währinger Gürtel 18-20, Postfach 120, A-1097, Vienna, Austria. heartreza@hotmail.com
authors.forename: M R
authors.initials: MR
authors.lastname: Mehrabi
authors.forename: N
authors.initials: N
authors.lastname: Serbecic
authors.forename: C
authors.initials: C
authors.lastname: Ekmekcioglu
authors.forename: F
authors.initials: F
authors.lastname: Tamaddon
authors.forename: R
authors.initials: R
authors.lastname: Ullrich
authors.forename: H
authors.initials: H
authors.lastname: Sinzinger
authors.forename: H D
authors.initials: HD
authors.lastname: Glogar
mesh_terms: D000328:Adult; D000368:Aged; D001021:Aortic Valve / Q000378:metabolism* / Q000473:pathology*; D015415:Biomarkers; D009202:Cardiomyopathies / Q000378:metabolism* / Q000473:pathology*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011664:Pulmonary Valve / Q000378:metabolism* / Q000473:pathology*; D011863:Radioimmunoassay; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost