Adenoviral E1A protein nuclear import is preferentially mediated by importin alpha3 in vitro.
Nuclear import of some viral proteins depends on importin alpha proteins. However, no preferences of distinct alpha-importins for any viral protein import have been demonstrated. We used in vitro import assays and observed that all ubiquitously expressed human importin alpha isoforms mediate nuclear translocation of adenoviral E1A. Competition with nucleoplasmin suggests that importin alpha3 is the most efficient import mediator of E1A.
Köhler
M
M
HELIOS Clinic/Franz Volhard Clinic/Max Delbrueck Center, Humboldt University Berlin, Berlin, 13122, Germany.
Görlich
D
D
Hartmann
E
E
Franke
J
J
D017500:Adenovirus E1A Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D004265:DNA Helicases / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D056489:Nucleoplasmins; D010750:Phosphoproteins / Q000378:metabolism; D021381:Protein Transport; D028901:alpha Karyopherins / Q000378:metabolism*
D017500:Adenovirus E1A Proteins; C106259:KPNA4 protein, human; D009687:Nuclear Proteins; D056489:Nucleoplasmins; D010750:Phosphoproteins; D028901:alpha Karyopherins; D004265:DNA Helicases