title: Expression, purification, crystallization and preliminary X-ray analysis of the cathelicidin motif of the protegrin-3 precursor.
abstract: Numerous precursors of antibacterial peptides with unrelated sequences share a similar prosequence which belongs to the cathelicidin family of proteins. The three-dimensional structure of this cathelicidin motif, which contains two disulfide bonds, has not yet been reported. The cathelicidin motif (ProS) of the protegrin-3 precursor was overexpressed in Escherichia coli as a His-tagged protein. The His(6) tag was removed by thrombin cleavage. ProS was purified to homogeneity and single crystals were obtained by the hanging-drop vapour-diffusion method at pH 3-4. Preliminary X-ray diffraction analysis indicated that these crystals belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 51.42, c = 134.25 A. These crystals diffracted beyond 2.75 A (1.9 A at ESRF) and contain one molecule per asymmetric unit.
authors.affiliation: Centre de Biochimie Structurale, UMR 5048 CNRS-UM1/UMR 554 INSERM-UM1, Université Montpellier 1, Faculté de Pharmacie, 15 Avenue Charles Flahault, 34060 Montpellier CEDEX 2, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Sanchez
authors.forename: F
authors.initials: F
authors.lastname: Hoh
authors.forename: M P
authors.initials: MP
authors.lastname: Strub
authors.forename: J M
authors.initials: JM
authors.lastname: Strub
authors.forename: A
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: R
authors.initials: R
authors.lastname: Lehrer
authors.forename: T
authors.initials: T
authors.lastname: Ganz
authors.forename: A
authors.initials: A
authors.lastname: Chavanieu
authors.forename: B
authors.initials: B
authors.lastname: Calas
authors.forename: C
authors.initials: C
authors.lastname: Dumas
authors.forename: A
authors.initials: A
authors.lastname: Aumelas
mesh_terms: D020816:Amino Acid Motifs; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D011498:Protein Precursors; D011506:Proteins; D011994:Recombinant Proteins; C121626:cathelicidin 3 protein, Equus caballus; C094881:protegrin-3