title: Cytotoxicity of aflatoxin B1 and its chemically synthesised epoxide derivative on the A549 human epithelioid lung cell line.
abstract: Aflatoxin B1 (AFB1 ) is a carcinogenic mycotoxin found in feeds and in airborne grain dusts. Aflatoxin B1 requires biotransformation to the AFB1-8,9 epoxide (AFBO) by a bioactivation system and subsequent covalent binding to DNA or proteins, to exert its carcinogenic potential. The lung contains cytochrome P450, prostaglandin-H-synthase, lipoxygenase, epoxide hydrolase and other bioactivation enzymes, and is thus a potential target for the effects of AFB1 via the routes of inhalation and ingestion. The A549 human epithelioid lung cell line and the methylthiazol tetrazolium (MTT) bioassay were used to investigate the cytotoxicity of AFB1 and its chemically synthesised epoxide (AFBO) in vitro. Statistical analysis of the MTT results indicated that there were overall significant differences between the control and both the AFB1-treated (p < 0.0001) and AFBO-treated cells (p = 0.002). However, there was no significant difference between AFB1 and AFBO-treated cells, when the entire range of concentrations were assessed against each other (p = 0.2877). When analysed at each concentration, only at 0.01 mM was there a significant difference between the effects of AFB1 and AFBO (p = 0.0358). The results of this investigation show that AFB1 and AFBO are both cytotoxic in the A549 cell line.
authors.affiliation: School of Medical Sciences, Nelson R. Mandela School of Medicine, University of Natal, Kwazulu-Natal, South Africa. 921320392@nu.ac.za
authors.forename: T
authors.initials: T
authors.lastname: Palanee
authors.forename: M F
authors.initials: MF
authors.lastname: Dutton
authors.forename: A A
authors.initials: AA
authors.lastname: Chuturgoon
mesh_terms: D016604:Aflatoxin B1 / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D015622:Epithelioid Cells / Q000187:drug effects; D005562:Formazans; D006801:Humans; D008168:Lung / Q000187:drug effects*; D013778:Tetrazolium Salts; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; C006426:aflatoxin B1-2,3-oxide; D016604:Aflatoxin B1