Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against Abeta-induced oxidative stress in PC12 cells.
Amyloid beta protein (Abeta)-induced free radical-mediated neurotoxicity is a leading hypothesis as a cause of Alzheimer's disease (AD). Abeta increased free radical production and lipid peroxidation in PC12 nerve cells, leading to apoptosis and cell death. The effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica on Abeta induced neurotoxicity was investigated using PC12 cells. Pretreatment with isolated 4',5-dihydroxy-3',6,7-trimethoxyflavone and vitamin E prevented the Abeta-induced reactive oxygen species (ROS). The 4',5-dihydroxy-3',6,7-trimethoxyflavone resulted in concentration-dependant decreased Abeta toxicity assessed by 3-(4, 5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. However, treatment with these antioxidants inhibited the Abeta-induced neurotoxic effect. Therefore, these results indicate that micromolecular Abeta-induced oxidative cell stress is reduced by 4,5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica.
Heo
H J
HJ
Graduate School of Biotechnology, Korea University, Seoul.
Cho
H Y
HY
Hong
B
B
Kim
H K
HK
Kim
E K
EK
Kim
B G
BG
Shin
D H
DH
D000313:Adrenal Medulla / Q000166:cytology; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D004347:Drug Interactions; D005089:Exocytosis / Q000187:drug effects* / Q000502:physiology; D047309:Flavones; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005562:Formazans / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010673:Pheochromocytoma; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014810:Vitamin E / Q000494:pharmacology*
D016229:Amyloid beta-Peptides; D047309:Flavones; D005419:Flavonoids; D005562:Formazans; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D014810:Vitamin E; C079782:MTT formazan; C057618:cirsilineol