Effect of sialyl Lewis(x) selectin blockade on myocardial protection during cardioplegic arrest and reperfusion.
Selectins play a crucial role in the neutrophil-mediated myocardial injury associated with ischemia/reperfusion. We investigated the effect of selectin inhibition on neutrophil-endothelial cell adhesion, myocardial water content, and left ventricular (LV) recovery after cardiopulmonary bypass (CPB) and cardioplegia.



Dogs were subjected to CPB and 60 minutes of hypothermic cardioplegia. A selectin inhibitor (SI) (25 mg/kg) was given five minutes prior to CPB and as a continuous infusion (5 mg/kg/h) throughout CPB (n = 6). Saline-treated controls (n = 6) received identical volumes. Preload recruitable stroke work (PRSW) was calculated by sonomicrometry and micromanometry. Myocardial water content was determined by microgravimetry. Myeloperoxidase (MPO) activity was measured to quantify polymorphonuclear neutrophil (PMN) infiltration.



SI did not attenuate PRSW as well as post-CPB MPO tissue activity. While we found no difference in myocardial water gain between groups 120 minutes post-CPB, there was better edema resolution with SI.



Selectin antagonism does not reduce CPB-associated myocardial injury, and contractile recovery is not enhanced.
Sauer
H H
HH
Department of Anesthesiology, University of Texas-Houston Medical School, USA. Henning.Sauer@ewetel.net
Allen
S J
SJ
Cox
C S
CS
Laine
G A
GA
D000818:Animals; D001713:Biphenyl Compounds / Q000627:therapeutic use*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004285:Dogs; D004341:Drug Evaluation; D006324:Heart Arrest, Induced / Q000009:adverse effects*; D008358:Mannose / Q000031:analogs & derivatives; D008362:Mannosides / Q000627:therapeutic use*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D018375:Neutrophil Activation; D009844:Oligosaccharides; D019087:Selectins; D000080506:Sialyl Lewis X Antigen
D001713:Biphenyl Compounds; D008362:Mannosides; D009844:Oligosaccharides; D019087:Selectins; D000080506:Sialyl Lewis X Antigen; C108700:bimosiamose disodium; D008358:Mannose