Antitumor effect of the idiotypic cascade induced by an antibody encapsulated in poly(d,l-lactide-co-glycolide) microspheres.
A major difficulty encountered during development of antibody vaccines is their weak immunogenicity. In this study, a monoclonal antibody CS20.5 to human breast cancer antigen CA15.3 was coencapsulated in poly(d,l-lactide-co-glycolide) microspheres with monophosphoryl lipid A. The antitumor effect of this formulation was investigated in a murine model. The induced Ab2 biologically mimics antigen as it competed with CA15.3 for the same idiotope on Ab1. Ab3 induction was also observed. After five sequential administrations of encapsulated antibody, mice showed statistically significant tumor regression. These results indicate that this formulation may serve as a potential treatment for breast cancer.
Ma
J
J
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Panjiayuan, Beijing, China 100021. jiema2000@yahoo.com
Luo
D
D
Qi
W
W
Cao
L
L
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D000912:Antibodies, Neoplasm / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000276:immunology; D001943:Breast Neoplasms / Q000276:immunology* / Q000628:therapy*; D004337:Drug Carriers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D019344:Lactic Acid; D051379:Mice; D008863:Microspheres; D009368:Neoplasm Transplantation; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000970:Antineoplastic Agents; D004337:Drug Carriers; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid