title: Fibroblasts and monocyte macrophages contract and degrade three-dimensional collagen gels in extended co-culture.
abstract: BACKGROUND
  Inflammatory cells are believed to play a prominent role during tissue repair and remodeling. Since repair processes develop and mature over extended time frames, the present study was designed to evaluate the effect of monocytes and fibroblasts in prolonged culture in three-dimensional collagen gels.
  
  
  METHODS
  Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks.
  
  
  RESULTS
  Fibroblast-mediated gel contraction was initially inhibited by the presence of monocytes (P < 0.01). However, with extended co-culture, contraction of the collagen gels was greatly augmented (P < 0.01). In addition, with extended co-culture, degradation of collagen in the gels occurred. The addition of neutrophil elastase to the medium augmented both contraction and degradation (P < 0.01). Prostaglandin E2 production was significantly increased by co-culture and its presence attenuated collagen degradation.
  
  
  CONCLUSIONS
  The current study, therefore, demonstrates that interaction between monocytes and fibroblasts can contract and degrade extracellular matrix in extended culture.
authors.affiliation: University of Nebraska Medical Center, 985125 Nebraska Medical Center, Omaha, NE 68198-5125, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: C M
authors.initials: CM
authors.lastname: Sköld
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Kohyama
authors.forename: F Q
authors.initials: FQ
authors.lastname: Wen
authors.forename: R F
authors.initials: RF
authors.lastname: Ertl
authors.forename: S I
authors.initials: SI
authors.lastname: Rennard
mesh_terms: D002460:Cell Line; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D005782:Gels; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D019272:Leukocyte Elastase / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005782:Gels; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen; D019272:Leukocyte Elastase; D015232:Dinoprostone; D006909:Hydroxyproline