title: Evaluation of phagocytic activity in human monocyte-derived dendritic cells.
abstract: Dendritic cells play a central role in initiation of primary T lymphocyte responses to foreign antigens. Their potency in antigen presentation vis-à-vis reported low or lack of ability to phagocytize particulate matter has limited our understanding of the role that they play in inducing immunity to particulate antigens. One hypothesis is that dendritic cells may possess a high phagocytic capacity when immature and located in peripheral tissues, which they lose on maturation. Our goal was to characterize the phagocytic capacity in human immature dendritic cells. The phagocytic capacity of human monocyte-derived immature dendritic cells was studied by morphological and morphometric means, and compared to that of professional phagocytes, human alveolar macrophages, their progenitors, the peripheral blood monocytes, and mature dendritic cells. Phagocytic index (proportion of phagocytic cells) was decreased by 42.8% (immature dendritic cells) and 74.2% (mature dendritic cells) with respect to monocytes. Similarly, the phagocytic index was decreased by 46.5% (immature dendritic cells) and 75.9% (mature dendritic cells) with respect to macrophages. Volume density of phagocytized particles was decreased by 76.1% (immature dendritic cells) and 96.7% (mature dendritic cells) with respect to the monocytes. However, volume density was decreased by 34.3% (immature dendritic cells) and 91% (mature dendritic cells) with respect to alveolar macrophages. These results show that human monocyte-derived immature dendritic cells possess a phagocytic capacity that is lower than that of peripheral blood monocytes and alveolar macrophages but higher than that of mature dendritic cells.
authors.affiliation: Institute of Anatomy, University of Berne, Switzerland.
authors.forename: S G
authors.initials: SG
authors.lastname: Kiama
authors.forename: L
authors.initials: L
authors.lastname: Cochand
authors.forename: L
authors.initials: L
authors.lastname: Karlsson
authors.forename: L P
authors.initials: LP
authors.lastname: Nicod
authors.forename: P
authors.initials: P
authors.lastname: Gehr
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D016676:Macrophages, Alveolar / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D010316:Particle Size; D010587:Phagocytosis