On-chip capillary electrophoresis for alkaline phosphatase testing.
We fabricated an on-chip capillary electrophoresis device for blood analysis. An on-chip capillary electrophoresis device was photolithographically fabricated on a glass chip. Alkaline phosphatase (ALP) was employed as a sample enzyme. Small amounts of enzyme in the mixture of other proteins were detected with the electrophoretically mediated microanalysis (EMMA) method. Fluorescein diphosphate was used as fluorogenic substrate. The detection of ALP activity was achieved with laser-induced fluorescence monitoring fluorescein that was produced in enzyme reaction in capillary. Several methods to reduce the adhesion of protein are also discussed.
Murakami
Y
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Tatsunokuchi, Ishikawa 923-1292, Japan. yuji@jaist.ac.jp
Morita
T
T
Kanekiyo
T
T
Tamiya
E
E
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans
D005452:Fluoresceins; D005456:Fluorescent Dyes; C074818:3,6-fluorescein diphosphate; D000469:Alkaline Phosphatase