Metabolic engineering for microbial production of aromatic amino acids and derived compounds.
Metabolic engineering to design and construct microorganisms suitable for the production of aromatic amino acids and derivatives thereof requires control of a complicated network of metabolic reactions that partly act in parallel and frequently are in rapid equilibrium. Engineering the regulatory circuits, the uptake of carbon, the glycolytic pathway, the pentose phosphate pathway, and the common aromatic amino acid pathway as well as amino acid importers and exporters that have all been targeted to effect higher productivities of these compounds are discussed.
Bongaerts
J
J
DSM Biotech GmbH, Karl-Heinz-Beckurts-Strasse 13, D-52428 Jülich, Germany.
Krämer
M
M
Müller
U
U
Raeven
L
L
Wubbolts
M
M
D024322:Amino Acids, Aromatic / Q000096:biosynthesis*; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005246:Feedback; D005818:Genetic Engineering; D010728:Phosphoenolpyruvate / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
D024322:Amino Acids, Aromatic; D013403:Sugar Phosphates; C026959:erythrose 4-phosphate; D010728:Phosphoenolpyruvate