Preparation of an antibody recognizing both human and rodent MRP1.
Based on the high level of identity among human, mouse, and rat MRP1 protein sequence, we produced a specific polyclonal antibody (MRP1-A23) against a synthetic polypeptide covering the C-terminus of the human protein. Western blot analysis showed a reactivity against human MRP1 similar to that obtained with the monoclonal QCRL1 antibody. Differently from other available antibodies against human MPR1, MRP1-A23 also detected both rat and mouse MRP1. No cross-reactivity was observed with either human or mouse MRP2 while MRP1-A23 weakly cross-reacted with rat MRP2 in the protein region ranging from 1512 to 1533 amino acids. These data indicate that MRP1-A23 allows specific MRP1 detection in both human and rodent tissues and may provide an important tool in the study of MRP1 expression and function in both experimental and clinical materials.
Fernetti
C
C
CSF and Department of BBCM, Centro Servizi Polivalenti d'Ateneo, University of Trieste, Via Giorgieri 1, Trieste, 34127, Italy.
Pascolo
L
L
Podda
E
E
Gennaro
R
R
Stebel
M
M
Tiribelli
C
C
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D017124:Conserved Sequence; D003429:Cross Reactions / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D012377:Rodentia / Q000276:immunology*; D016415:Sequence Alignment
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007106:Immune Sera; D026901:Membrane Transport Proteins; D000089762:Multidrug Resistance-Associated Protein 2; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010455:Peptides; C000715087:ABCC2 protein, human; C421109:multidrug resistance-associated protein 1