title: Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction.
abstract: We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.
authors.affiliation: Centre International de Recherches Médicales de Franceville, Franceville, Gabon. toure@cirmf.sc.ga
authors.forename: F S
authors.initials: FS
authors.lastname: Touré
authors.forename: E
authors.initials: E
authors.lastname: Mavoungou
authors.forename: J M
authors.initials: JM
authors.lastname: Ndong
authors.forename: P
authors.initials: P
authors.lastname: Tshipamba
authors.forename: P
authors.initials: P
authors.lastname: Deloron
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000592:standards*; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; D002352:Carrier Proteins; D017931:DNA Primers; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium