title: Transmission blocking vaccine studies in leishmaniasis: II. Effect of immunisation using Leishmania major derived 63 kilodalton glycoprotein, lipophosphoglycan and whole parasite antigens on the course of L. major infection in BALB/c mice.
abstract: BACKGROUND
  Safe, effective and inexpensive vaccines may be the most practical tool for control of any form of leishmaniasis. Leishmaniasis produces a state of pre-immunition which is the underlying mechanism for prolonged immunity to re-infection. Low doses of parasites has been shown to be able to induce protection in mice. It is not known, however, how immune sera from a susceptible host immunised with Leishmania-derived antigens when taken in by the sandfly affects the development and the subsequent transmission of the parasite to naive hosts.
  
  
  OBJECTIVE
  To monitor the course of disease in BALB/c mice following challenge using L. major infected P. duboscqi which had previously fed on immunised mice.
  
  
  METHODS
  BALB/c mice were immunised adequately with Leishmania major-derived antigens namely, crude whole parasite (WPA), recombinant 63 kilodalton glycoprotein (rgp63), lipophosphoglycan (LPG) and a cocktail composed of rgp63 plus LPG antigens. Laboratory reared Phlebotomus duboscqi sandflies, the natural vector for L. major were later allowed to feed on immunised animals, interrupted and allowed to continue feeding on infected animals for an equal amount of time until they became fully engorged. The sandflies were maintained on apples as a carbohydrate source in an insectary maintained at a temperature of 25 degrees C and 80% relative humidity. On the seventh day these sandflies were used to infect naive BALB/c mice and the course of infection followed for a period of at least three months.
  
  
  RESULTS
  Mice infected using sandflies which had previously fed on WPA or rgp63-immunized mice showed disease exacerbation as the infection progressed, whereas those infected using sandflies which had previously fed on LPG-immunised mice had the least lesion sizes compared to control mice infected using sandflies which had fed on saline immunised mice (p < 0.05).
  
  
  CONCLUSIONS
  Results from this study indicate that the course of L. major infection in BALB/c mice was dependent on the infective dose of parasites transmitted by the sandflies. Results from this study suggests that sub-infective doses of the parasite from sandflies previously fed on animals immunised with Leishmania-derived antigens needs to be evaluated for their potential in vaccine development against Leishmania infections.
authors.affiliation: Centre for Biotechnology Research and Development, Kenya Medical Research Institute, P.O. Box 54840, Nairobi, Kenya.
authors.forename: W K
authors.initials: WK
authors.lastname: Tonui
authors.forename: P A
authors.initials: PA
authors.lastname: Mbati
authors.forename: C O
authors.initials: CO
authors.lastname: Anjili
authors.forename: A S
authors.initials: AS
authors.lastname: Orago
authors.forename: S J
authors.initials: SJ
authors.lastname: Turco
authors.forename: J I
authors.initials: JI
authors.lastname: Githure
authors.forename: D K
authors.initials: DK
authors.lastname: Koech
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004195:Disease Models, Animal; D018450:Disease Progression; D004353:Drug Evaluation, Preclinical; D006028:Glycosphingolipids / Q000276:immunology*; D007303:Insect Vectors / Q000469:parasitology; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000635:transmission*; D008666:Metalloendopeptidases / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010691:Phlebotomus / Q000469:parasitology; D016052:Protozoan Vaccines / Q000276:immunology*; D013997:Time Factors
chemical_list: D000953:Antigens, Protozoan; D006028:Glycosphingolipids; D016052:Protozoan Vaccines; C008290:lipophosphonoglycan; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania