Identification of novel genes coding for small expressed RNAs.
In Caenorhabditis elegans, lin-4 and let-7 encode 22- and 21-nucleotide (nt) RNAs, respectively, which function as key regulators of developmental timing. Because the appearance of these short RNAs is regulated during development, they are also referred to as small temporal RNAs (stRNAs). We show that many 21- and 22-nt expressed RNAs, termed microRNAs, exist in invertebrates and vertebrates and that some of these novel RNAs, similar to let-7 stRNA, are highly conserved. This suggests that sequence-specific, posttranscriptional regulatory mechanisms mediated by small RNAs are more general than previously appreciated.
Lagos-Quintana
M
M
Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D-37077 Göttingen, Germany.
Rauhut
R
R
Lendeckel
W
W
Tuschl
T
T
D000818:Animals; D020029:Base Pairing; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D019295:Computational Biology; D017124:Conserved Sequence; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006367:HeLa Cells; D006801:Humans; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D012313:RNA / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D012322:RNA Precursors; D022661:RNA, Untranslated; D012313:RNA