Detection of mutations within the thymidine kinase gene of herpes simplex virus type 1 by denaturing gradient gel electrophoresis.
Denaturing gradient gel electrophoresis has been applied widely for the detection of mutation(s) and polymorphisms of various genes. It is shown that this system is also feasible for analyzing mutations in the thymidine kinase (tk) gene of herpes simplex virus type 1 (HSV-1). Thus, this system is applicable for examining whether a drug-resistant HSV-1 contains a tk mutation(s) or otherwise. This system can also be useful for detecting heterogeneity and the emergence of drug-resistant mutants in clinical samples.
Lu
Qiaosheng
Q
Department of Microbiology and Immunology, Medical College, Upstate Medical University, State University of New York, Syracuse, NY 13210, USA.
Hwang
Ying T
YT
Hwang
Charles B C
CB
D004587:Electrophoresis, Agar Gel; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013937:Thymidine Kinase / Q000235:genetics*
D013937:Thymidine Kinase