Erythrocyte binding antigen (EBA-175) of Plasmodium falciparum: improved genotype determination by nested polymerase chain reaction.
We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)-175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.
Touré
F S
FS
Centre International de Recherches Médicales de Franceville, Franceville, Gabon. toure@cirmf.sc.ga
Mavoungou
E
E
Ndong
J M
JM
Tshipamba
P
P
Deloron
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016778:Malaria, Falciparum / Q000453:epidemiology; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000592:standards*; D015800:Protozoan Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan; D002352:Carrier Proteins; D017931:DNA Primers; D015800:Protozoan Proteins; C065668:erythrocyte-binding antigen 175, Plasmodium