title: Deletion mutagenesis of large (12-kb) plasmids by a one-step PCR protocol.
authors.affiliation: The University of Texas M.D. Anderson Cancer Center, Houston, 77030, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: M F
authors.initials: MF
authors.lastname: Wilkinson
mesh_terms: D000818:Animals; D001483:Base Sequence; D001709:Biotechnology; D017931:DNA Primers / Q000235:genetics; D051379:Mice; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D011948:Receptors, Antigen, T-Cell