Production of Acinetobacter radioresistens lipase with repeated batch culture in presence of nonwoven fabric.
Cultivation of Acinetobacter radioresistens on n-hexadecane for lipase production was investigated with repeated batch culture in the presence of a hydrophobic nonwoven fabric. Lipase production followed the growth-associated model, and the repeated batch culture could achieve both high enzyme yield and increased volumetric productivity. The fabric was shown to be able to disperse n-hexadecane, to adsorb the unused hydrocarbon, and to retain bioemulsifiers excreted from the cells; therefore, it enhanced cell growth and, in turn, lipase production. In the repeated batch culture in the absence of the fabric, lipase yield and volumetric productivity were found to be 21 U/mL and 875 U/L. h, respectively. However, if the fabric was equipped in the fermentor, lipase yield and volumetric productivity increased to 30 U/mL and 2500 U/L. h, respectively. The lipase production profile could be further improved by raising the amount of nitrogen source and, as a result, a lipase yield of 54 U/mL and a volumetric productivity of 2250 U/L. h were obtained. In this study we assess the beneficial effects of nonwoven fabric on lipase production.
Lin
Y C
YC
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan. t62660@mail.ncku.edu.tw
Wu
J Y
JY
Chen
T L
TL
D000150:Acinetobacter / Q000201:enzymology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D005285:Fermentation; D008049:Lipase / Q000096:biosynthesis*; D017672:Nitrogen Compounds / Q000378:metabolism
D017672:Nitrogen Compounds; D008049:Lipase