[Expression of HBsAg with four eukaryotic expression plasmids in immortalized B-cell line from a chronic hepatitis B patient].
OBJECTIVE
To establish efficient eukaryotic expression system of HBsAg in immortalized B-cell line from a chronic hepatitis B patient, as autologous HLA target cells for detecting HBsAg-specific cytotoxic T-lymphocyte (CTL) response.


METHODS
Four eukaryotic expression plasmids inserted HBsAg gene were transfected into immortalized B-cell line from a chronic hepatitis B patient, then cells were selected with G418 or hygromycin B. HBsAg in culture supernatants and cell lysates were detected with enzyme-linked immunosorbent assay (ELISA).


RESULTS
All eukaryotic expression vectors-transfected immortalized B-cells produced HBsAg, which was readily detectable in culture supernatants and cell lysates.


CONCLUSIONS
Transfection of EBV-immortalized B-cell line with four eukaryotic expression plasmids leads to stable expression of HBsAg, which can be used as autologous HLA target cells for detecting HBsAg-specific CTL response.
Liang
Z
Z
Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Li
Y
Y
Lan
Y
Y
Ren
H
H
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002472:Cell Transformation, Viral; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000276:immunology / Q000473:pathology*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D008297:Male; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D006514:Hepatitis B Surface Antigens