Separation and retention of human blood cells by surface-affinity chromatography.
This review describes the surface-affinity chromatography of human peripheral blood cells on polyethylene glycol (PEG) and polypropylene glycol (PPG) chemically bonded columns. The affinity of surface blood cells to bonded PEG and PPG stationary phases is apparently selective, and granulocytes and lymphocytes are more strongly retained on the column than erythrocytes and platelets. The retention factors of granulocytes and lymphocytes increased with increase in the hydrophobicities (Delta log K values) of PPG-agarose column packing beads. Hydrophobic interactions contributed to the retention of the blood cells on the PPG-bonded agarose columns.
Shibusawa
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School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan. sibusawa@ps.toyaku.ac.jp
D002846:Chromatography, Affinity / Q000379:methods; D016238:Cytapheresis / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008214:Lymphocytes / Q000166:cytology; D011092:Polyethylene Glycols; D019946:Propylene Glycol; D013499:Surface Properties
D011092:Polyethylene Glycols; D019946:Propylene Glycol