Basal level glucose-6-phosphatase gene transcription requires binding sites for Sp family proteins within the gene promoter.
The significance of two regions (SpA: -19 to -11 and SpB: -63 to -55) within the human glucose-6-phosphatase (G6Pase) gene promoter for gene expression was examined. The mutation of SpA and SpB together, but not alone, decreased G6Pase promoter activity. Electromobility shift assays showed that SpA and SpB were able to bind the transcription factors Sp1 and Sp3.
Wasner
C
C
Department of Medical Biochemistry and Molecular Biology, University of Greifswald, Sauerbruchstrasse, D-17487, Greifswald, Germany.
Grempler
R
R
Walther
R
R
Schmoll
D
D
D001483:Base Sequence; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005952:Glucose-6-Phosphatase / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C497121:SP3 protein, human; D014157:Transcription Factors; D051705:Sp3 Transcription Factor; D005952:Glucose-6-Phosphatase