Crystals of ternary protein-DNA complexes composed of DNA-binding domains of c-Myb or v-Myb, C/EBPalpha or C/EBPbeta and tom-1A promoter fragment.
c-Myb and the C/EBP family are transcriptional regulatory factors that act in concert to regulate the expression of myeloid-specific genes. v-Myb encoded by avian myeloblastosis virus (AMV) is a mutated form of c-Myb that contains point mutations which disrupt the cooperation with C/EBPs. To understand the mechanism of the transcriptional synergy between c-Myb and C/EBPs and the effect of the v-Myb mutations on that synergy, knowledge based on their three-dimensional structures is essential. Crystals of ternary complexes, in which various combinations of the DNA-binding domains of c-Myb or v-Myb and C/EBPalpha or C/EBPbeta are bound to a DNA fragment from tom-1A promoter, were obtained by the vapour-diffusion method. Complete diffraction data sets were obtained from each native crystal and two types of iodine-derivative crystals. A three-wavelength MAD data set was also obtained from a bromine-derivative crystal.
Tahirov
T H
TH
Kanagawa Academy of Science and Technology (KAST), Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. tahir@med.yokohama-cu.ac.jp
Sasaki
M
M
Inoue-Bungo
T
T
Fujikawa
A
A
Sato
K
K
Kumasaka
T
T
Yamamoto
M
M
Ogata
K
K
D000818:Animals; D001435:Bacteriophages / Q000737:chemistry; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000737:chemistry*; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D009690:Nucleic Acid Conformation; D020626:Oncogene Proteins v-myb / Q000737:chemistry; D011401:Promoter Regions, Genetic / Q000502:physiology; D011487:Protein Conformation; D011506:Proteins / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry*; D051381:Rats
D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; D047908:Intracellular Signaling Peptides and Proteins; D020626:Oncogene Proteins v-myb; D011506:Proteins; D020598:Proto-Oncogene Proteins c-myb; C120296:TOM1 protein, human