title: PMN responses following use of 2 biodegradable GTR membranes.
abstract: OBJECTIVE
  In the present prospective trial, the PMN response following resorbable GTR barrier placement was evaluated in mandibular class II furcation lesions.
  
  
  METHODS
  In 10 patients with treated chronic periodontitis, we randomly selected the 1st molars in the mandible with buccal degree II furcation involvement for either polylactic-citric-acid-ester (PLA) or glycolide-lactic-copolymer (PGL) GTR membrane therapy. We examined contralateral healthy molar sites as untreated controls. We then evaluated the PMN-derived inflammatory tissue response at baseline, weekly up to 6 weeks post-therapy and at 12 and 24 weeks using GCF myeloperoxidase (MPO), beta-glucuronidase (betaG) and beta-N-acetyl-hexosaminidase (betaNAH).
  
  
  RESULTS
  The enzyme levels increased from baseline to the 6-week examination. After the 6-week reappointment, enzyme levels dropped reaching the baseline scores at both the 12- and 24-week visit. At PGL sites, the enzyme levels decreased earlier. Compared with healthy control sites, the MPO, betaNAH and betaG tests revealed different maximum levels at week 2 and 3 (PGL) and week 4, 5 and 6 (PLA). For both of the barriers the clinical parameters revealed a sustained improvement following therapy.
  
  
  CONCLUSIONS
  The release of PMN enzymes following placement of bioabsorbable membranes reflects the early soft tissue healing process. Our results suggest that the PMN response is barrier-dependent with the maximum response occuring at different times. However, the host response did not measureably affect the course of clinical healing.
authors.affiliation: Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, MA 02118, USA. buchmann@bu.edu
authors.forename: R
authors.initials: R
authors.lastname: Buchmann
authors.forename: A
authors.initials: A
authors.lastname: Hasilik
authors.forename: A
authors.initials: A
authors.lastname: Heinecke
authors.forename: D E
authors.initials: DE
authors.lastname: Lange
mesh_terms: D020341:Absorbable Implants; D002908:Chronic Disease; D003774:Dental Plaque Index; D005260:Female; D005500:Follow-Up Studies; D017823:Furcation Defects / Q000145:classification / Q000601:surgery; D005883:Gingival Crevicular Fluid / Q000166:cytology / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation*; D006801:Humans; D019344:Lactic Acid; D008297:Male; D008336:Mandibular Diseases / Q000145:classification / Q000601:surgery; D008567:Membranes, Artificial; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology / Q000502:physiology*; D017622:Periodontal Attachment Loss / Q000601:surgery; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000601:surgery; D010518:Periodontitis / Q000601:surgery; D009195:Peroxidase / Q000032:analysis; D011091:Polyesters; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D014945:Wound Healing; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D009195:Peroxidase; D005966:Glucuronidase; D001619:beta-N-Acetylhexosaminidases