Expression of the MT1 melatonin receptor subtype in human coronary arteries.
Previous experimental data suggest a possible influence of melatonin on the circulatory system of animals after binding to G-protein coupled melatonin receptors. The present study sought to investigate whether the melatonin receptor, mt1, is expressed in human coronary arteries derived from healthy heart donors (n = 8). Expression of the mt1-receptor was studied in sections of isolated coronary arteries by a reverse transcriptase-polymerase chain reaction (RT-PCR) and Western immunoblot technique. The analyses of the results from both methods indicated the presence of the mt1-receptor in all of the subjects. Referring to these data we assume that melatonin regulates physiological processes in human coronary arteries after receptor binding.
Ekmekcioglu
C
C
Department of Physiology, University of Vienna, Medical School, Schwarzspanierstrasse 17, A- 1090 Vienna, Austria. cem.ekmekcioglu@univie.ac.at
Haslmayer
P
P
Philipp
C
C
Mehrabi
M R
MR
Glogar
H D
HD
Grimm
M
M
Leibetseder
V J
VJ
Thalhammer
T
T
Marktl
W
W
D001483:Base Sequence; D015153:Blotting, Western; D003331:Coronary Vessels / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008550:Melatonin / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D044094:Receptors, Melatonin; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin; D012313:RNA; D008550:Melatonin