Hydrolysis of oxidized nucleotides by the Escherichia coli Orf135 protein.
To examine the possibility that the Orf135 protein of Escherichia coli functions as a hydrolyzing enzyme for a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate), we purified the recombinant Orf135 protein and incubated it with oxidized deoxynucleotides. Of the nucleotides tested, 2-hydroxydeoxyadenosine 5'-triphosphate, and somewhat less efficiently, 8-hydroxydeoxyguanosine 5'-triphosphate, were hydrolyzed by this protein. These damaged deoxynucleotides elicit transversion mutations in E. coli (Inoue, M., Kamiya, H., Fujikawa, K., Ootsuyama, Y., Murata-Kamiya, N., Osaki, T., Yasumoto, K., Kasai, H. (1998) J. Biol. Chem. 273, 11069-11074). These results suggest that this protein may be involved in the prevention of mutations induced by these oxidized deoxynucleotides.
Kamiya
H
H
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo, 060-0812, Japan. hirokam@pharm.hokudai.ac.jp
Murata-Kamiya
N
N
Iida
E
E
Harashima
H
H
D004249:DNA Damage; D003838:Deoxyadenine Nucleotides / Q000737:chemistry / Q000494:pharmacology; D003848:Deoxyguanine Nucleotides / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D006868:Hydrolysis; D009711:Nucleotides / Q000378:metabolism*; D010084:Oxidation-Reduction; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000096964:Nudix Hydrolases
D003838:Deoxyadenine Nucleotides; D003848:Deoxyguanine Nucleotides; D009711:Nucleotides; D011755:Pyrophosphatases; C018891:deoxyadenosine diphosphate; C422942:nudix hydrolase Orf135; D000096964:Nudix Hydrolases