Expression of the cdc25B mRNA correlated with that of N-myc in neuroblastoma.
BACKGROUND
Neuroblastoma is one of the most common solid tumors in early childhood. Overexpression of the proto-oncogene N-myc has been reported to be correlated with more malignant course of the disease. cdc25B is reported to be a target of myc and elevated in several malignant cells and tissues.


METHODS
Expression of cdc25B and N-myc messenger RNAs were evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) assay in 20 tumor samples from neuroblastoma using LightCycler. The data were analyzed with reference to clinicopathological factors. Immunohistochemistry for cdc25B was also performed.


RESULTS
There was no significant difference in the cdc25B expression between patient groups according to age, gender and clinical stage. The cdc25B mRNA expression levels were significantly correlated with N-myc mRNA levels (y = -0.445 + 20.577x, p < 0.0001).


CONCLUSIONS
We could not establish the clinical significance to determine the cdc25B mRNA level from neuroblastoma. However, we suggest that cdc25B may play an active role as a target of N-myc in neuroblastoma, although the biological function of cdc25B in neuroblastoma remains to be clarified.
Sato
Y
Y
Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan.
Sasaki
H
H
Kondo
S
S
Fukai
I
I
Kiriyama
M
M
Yamakawa
Y
Y
Fujii
Y
Y
D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D016259:Genes, myc; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020687:cdc25 Phosphatases / Q000096:biosynthesis* / Q000235:genetics
D018797:Cell Cycle Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; C517008:CDC25B protein, human; D020687:cdc25 Phosphatases