title: The bacteriophage lambda attachment site in wild strains of Escherichia coli.
abstract: The attachment site (attlambda) of bacteriophage lambda was examined in wild strains of Escherichia coli. Although the att region is non-coding, the DNA sequence was invariant in the 13 strains examined. Two other non-coding regions showed nine changes, all associated with a single strain. In four of 33 strains, sequences were inserted in or near the attlambda site and in two of these the insert was related to lambda. Among strains that can be lysogenized by lambda, integration was via the attlambda site in all cases. Some resistant strains can be lysogenized, and these have been termed "lenient." Most of these fail to give normal phage yield after induction. In some cases rare lysogens have been formed in cells that belong to a mutant subpopulation.
authors.affiliation: Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kuhn
authors.forename: A
authors.initials: A
authors.lastname: Campbell
mesh_terms: D010582:Bacteriophage lambda / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000821:virology*; D014644:Genetic Variation; D008242:Lysogeny; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral