title: A method to obtain right-side-out and sealed plasma membrane vesicles from the filamentous fungus Penicillium simplicissimum.
abstract: Plasma membrane vesicles were purified from the citrate excreting filamentous fungus Penicillium simplicissimum. The hyphae were disrupted by grinding in a glass bead mill and plasma membranes were obtained from a crude membrane fraction by partitioning in an aqueous dextran/polyethylene glycol two-phase system. The resulting enriched plasma membrane fraction contained only minor contaminations of marker enzymes of intracellular membranes, consisted of more than 90% of right-side-out vesicles and was sealed for potassium.
authors.affiliation: Institute of Microbiology, Technikerstrasse 25, 6020 Innsbruck, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Zanella
authors.forename: W
authors.initials: W
authors.lastname: Burgstaller
mesh_terms: D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000648:ultrastructure*; D002951:Citrates / Q000378:metabolism; D007425:Intracellular Membranes; D008854:Microscopy, Electron; D010407:Penicillium / Q000378:metabolism / Q000648:ultrastructure*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D022161:Transport Vesicles / Q000648:ultrastructure*
chemical_list: D002951:Citrates; D006180:Proton-Translocating ATPases