In vivo detection of 13C-enriched glucose metabolites in mouse brain by T-SEDOR imaging.
Protons J-coupled to 13C were selectively detected in the mouse head by in vivo 1H NMR imaging based on Twin Spin Echo DOuble Resonance (T-SEDOR) excitation. This pulse sequence combines a good chemical specificity with high sensitivity, requires no solvent pre-saturation and is well adapted to the imaging modality. 1H T-SEDOR maps of the mouse head allowed detection of areas of preferential accumulation of 13C-enriched compounds, upon repeated injections of uniformly 13C-labelled glucose, which induced hyperglycemia. The results demonstrated the feasibility, both in time scale and metabolite concentration, of applying T-SEDOR MRI for in vivo mapping brain areas characterized by enhanced rates of glucose uptake and/or accumulation of its metabolites.
Testa
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Laboratory of Cell Biology, Istituto Superiore di Sanità, V.le Regina Elena 299, 00161, Rome, Italy.
Casieri
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C
Canese
R
R
Carpinelli
G
G
Podo
F
F
De Luca
F
F
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D002247:Carbon Isotopes; D005240:Feasibility Studies; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA
D001786:Blood Glucose; D002247:Carbon Isotopes