title: HIV-1 subtypes in Denmark.
abstract: The objective of this study was to investigate the presence of non-subtype B HIV-1 in Denmark. The C2-V3-C3 region of the env gene from proviral DNA obtained from patients suspected of being infected with non-subtype B virus was PCR-amplified and directly sequenced. The DNA sequences were aligned with full-length HIV-1 reference strains from each subtype and analysed using the phylogenetic package PHYLIP 3.1. The neighbour-joining method was used with 100 bootstraps. Of the 144 patients included in this study C2-V3-C3 sequences were obtained from 129 patients (90%). The phylogenetic analyses showed that virus from 49 patients (38%) was subtype A, 39 (30%) subtype C, 9 (7%) subtype D, 14 (11%) subtype CRF01_AE, 16 (12%) subtype B, 1 (1%) subtype F and 1 (1%) subtype J. This study demonstrates that almost all subtypes can be detected in Denmark; all non-subtype B infections could be traced to countries with a high prevalence of non-subtype B virus.
authors.affiliation: Department of Virology, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: R
authors.initials: R
authors.lastname: Machuca
authors.forename: M
authors.initials: M
authors.lastname: Bøgh
authors.forename: M
authors.initials: M
authors.lastname: Salminen
authors.forename: J
authors.initials: J
authors.lastname: Gerstoft
authors.forename: B
authors.initials: B
authors.lastname: Kvinesdal
authors.forename: C
authors.initials: C
authors.lastname: Pedersen
authors.forename: N
authors.initials: N
authors.lastname: Obel
authors.forename: H
authors.initials: H
authors.lastname: Nielsen
authors.forename: C
authors.initials: C
authors.lastname: Nielsen
mesh_terms: D004279:DNA, Viral / Q000235:genetics*; D003718:Denmark / Q000453:epidemiology; D005260:Female; D005784:Gene Amplification; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010802:Phylogeny; D015995:Prevalence
chemical_list: D004279:DNA, Viral