title: Involvement of bovine lactoferrin metal saturation, sialic acid and protein fragments in the inhibition of rotavirus infection.
abstract: Although the antiviral activity of lactoferrin is one of the major biological functions of this iron binding protein, the mechanism of action is still under debate. We have investigated the role of metal binding, of sialic acid and of tryptic fragments of bovine lactoferrin (bLf) in the activity towards rotavirus (intestinal pathogen naked virus) infecting enterocyte-like cells. The antiviral activity of bLf fully saturated with manganese or zinc was slightly decreased compared to that observed for apo- or iron-saturated bLf. The antiviral activity of differently metal-saturated bLf towards rotavirus was exerted during and after the virus attachment step. The removal of sialic acid enhanced the anti-rotavirus activity of bLf. Among all the peptidic fragments obtained by tryptic digestion of bLf and characterised by advanced mass spectrometric methodologies, a large fragment (86-258) and a small peptide (324-329: YLTTLK) were able to inhibit rotavirus even if at lower extent than undigested bLf.
authors.affiliation: Department of Ultrastructures, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Superti
authors.forename: R
authors.initials: R
authors.lastname: Siciliano
authors.forename: B
authors.initials: B
authors.lastname: Rega
authors.forename: F
authors.initials: F
authors.lastname: Giansanti
authors.forename: P
authors.initials: P
authors.lastname: Valenti
authors.forename: G
authors.initials: G
authors.lastname: Antonini
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001059:Apoproteins / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D019073:HT29 Cells; D006801:Humans; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry*; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014357:Trypsin
chemical_list: D000998:Antiviral Agents; D001059:Apoproteins; D008670:Metals; D010446:Peptide Fragments; C039223:apolactoferrin; D007781:Lactoferrin; D014357:Trypsin; D019158:N-Acetylneuraminic Acid