title: Microarray expression profiling of tissues from mice with uniparental duplications of chromosomes 7 and 11 to identify imprinted genes.
abstract: Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this approach is to combine the new technology with established genetic tools to associate genes with specific biological function. In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes (Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and strain-matched samples and attention to detail in tissue dissection technique.
authors.affiliation: Division of Human Genetics, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Choi
authors.forename: L A
authors.initials: LA
authors.lastname: Underkoffler
authors.forename: J N
authors.initials: JN
authors.lastname: Collins
authors.forename: S M
authors.initials: SM
authors.lastname: Marchegiani
authors.forename: N A
authors.initials: NA
authors.lastname: Terry
authors.forename: C V
authors.initials: CV
authors.lastname: Beechey
authors.forename: R J
authors.initials: RJ
authors.lastname: Oakey
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics; D003433:Crosses, Genetic; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics
chemical_list: D012333:RNA, Messenger