Crystallization and preliminary X-ray diffraction analysis of glutathione-dependent dehydroascorbate reductase from spinach chloroplasts.
Glutathione-dependent dehydroascorbate reductase (GSH-DHAR) catalyzes the reduction of dehydroascorbate to ascorbate using reduced glutathione as the electron donor. GSH-DHAR from spinach chloroplasts produced in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals were monoclinic, space group C2, with unit-cell parameters a = 98.25, b = 39.96, c = 106.86 A, beta = 110.46 degrees. The asymmetric unit contained two molecules, giving a crystal volume per enzyme mass (V(M)) of 2.06 A(3) Da(-1) and a solvent content of 40.3%. A full set of X-ray diffraction data were collected to 2.2 A Bragg spacing from three native crystals with an overall R(merge) of 6.5% and a completeness of 93.4%.
Mizohata
E
E
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Kumei
M
M
Matsumura
H
H
Shimaoka
T
T
Miyake
C
C
Inoue
T
T
Yokota
A
A
Kai
Y
Y
D002736:Chloroplasts / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010088:Oxidoreductases / Q000737:chemistry*; D011487:Protein Conformation; D018724:Spinacia oleracea / Q000201:enzymology*
D010088:Oxidoreductases; C020666:glutathione dehydrogenase (ascorbate)