Microarray RNA quantification assay for large-scale nanosamples analysis.
We have developed a convenient and sensitive method for the quantification of RNA in samples from microbiopsies. This procedure is especially suitable for quantitating very small amounts of RNA in large-scale biological samples. This method, using a microarray-spotting facility for the study of multigenic expression, entails the hybridization of a DNA probe with RNA spotted at high density on nylon membrane. Furthermore, with this procedure, the reproducibility, sensitivity, and accuracy of the assay are notably improved as compared to current methods.
Dupré
I
I
Laboratoire de Neurosciences Précliniques, Inserm U318, Université J. Fourier, CHR Grenoble, France. isabelle.dupre@ujf-grenoble.fr
Atifi
M E
ME
Rostaing
B
B
Chambaz
E M
EM
Benabid
A L
AL
Berger
F
F
D001709:Biotechnology; D005260:Female; D006801:Humans; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes; D012313:RNA / Q000032:analysis* / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification
D015345:Oligonucleotide Probes; D012334:RNA, Neoplasm; D012313:RNA