Immunohistochemical detection of verotoxin receptors in nervous system.
Verotoxin receptor is a globotriaosylceramide (Gb3) present on vascular endothelial cells, in which the toxin causes hemolytic uremic syndrome. We established a sensitive method of immunohistochemical staining in serial sections using monoclonal antibodies (mAbs) against verotoxin and glycolipids that includes Gb3 and galactosylGb3 (GalGb3). With it, we showed co-localization of the verotoxin receptor and Gb3 in dorsal root ganglia (DRG) of humans, rabbits, rats and mice. We also used a very simple method to identify the presence of lipofuscin-like autofluorescence which complicates fluorescence microscopy observation of aged human nervous tissues.
Utsunomiya
I
I
Department of Neuroscience, Showa Pharmaceutical University, 3-3165 Higashitamagawagakuen, Machida, 194-8543, Tokyo, Japan.
Ren
J
J
Taguchi
K
K
Ariga
T
T
Tai
T
T
Ihara
Y
Y
Miyatake
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D005727:Ganglia, Spinal / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D022603:Shiga Toxins / Q000378:metabolism*; D013194:Staining and Labeling; D014018:Tissue Distribution; D014281:Trihexosylceramides / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D022603:Shiga Toxins; D014281:Trihexosylceramides; C018549:globotriaosylceramide