title: Short report: genetic heterogeneity of Japanese encephalitis virus assessed via analysis of the full-length genome sequence of a Korean isolate.
abstract: We determined the full-length genome sequence of Japanese encephalitis virus (JEV) K94P05 isolated in Korea. Sequence analysis showed that the 10,963-nucleotide-long RNA genome of K94P05 was 13 or 14 nucleotides shorter than the genome of other JEV isolates because of a deletion in the 3' noncoding region of K94P05. Compared with sequences of other JEV isolates, the full-length nucleotide sequence showed 89.0-89.6% homology, and the deduced amino acid sequence showed between 96.4-97.3% homology. A region of approximately 60 nucleotides immediately downstream of the open reading frame stop codon of K94P05 showed high sequence variability as compared with other JEV isolates. K94P05 formed a distinct group within a phylogenetic tree established with the full-length genome sequences. Cross-neutralization studies showed that polyclonal antibodies to Korean isolates were 3 times better at neutralizing the Korean isolates than antibodies to Nakayama-NIH. These findings suggest that Korean JEV K94P05 is genetically and antigenically distinct from other Asian JEV isolates.
authors.affiliation: Department of Virology, Korea National Institute of Health, Seoul.
authors.forename: J H
authors.initials: JH
authors.lastname: Nam
authors.forename: S L
authors.initials: SL
authors.lastname: Chae
authors.forename: S Y
authors.initials: SY
authors.lastname: Won
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.forename: K S
authors.initials: KS
authors.lastname: Yoon
authors.forename: B I
authors.initials: BI
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Jeong
authors.forename: H W
authors.initials: HW
authors.lastname: Cho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004279:DNA, Viral / Q000737:chemistry*; D004664:Encephalitis Virus, Japanese / Q000145:classification* / Q000235:genetics*; D004672:Encephalitis, Japanese / Q000821:virology; D016679:Genome, Viral; D006387:Hemagglutination, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral