Immunohistochemical detection of parathyroid hormone-related peptide, Indian hedgehog, and patched in the process of endochondral ossification in the human.
Parathyroid hormone-related peptide (PTHrP), Indian hedgehog (Ihh), and patched (Ptc; a receptor for Ihh) were immunolocalized in tissue undergoing endochondral ossification in the human. PTHrP, Ihh, and Ptc were immunolocalized in prehypertrophic and hypertrophic chondrocytes in mature cartilage matrix. PTHrP and Ptc were immunostained in proliferating chondrocytes and perichondrial cells, whereas Ihh was not. PTHrP, Ihh, and Ptc showed positive immunostaining in osteoblasts in the bone-forming area. In the bone resorption site, PTHrP was immunolocalized in osteoclasts, whereas Ihh and Ptc were not. The present findings indicated that PTHrP, Ihh, and Ptc were associated with the process of endochondral ossification, and suggested the possible involvement of Ihh and PTHrP signaling in the regulation of proliferation and hypertrophy of chondrocytes in human chondrogenesis.
Nakase
T
T
Department of Orthopaedic Surgery (E3), Osaka University Medical School, 2-2, Yamadaoka, Suita 565-0871, Japan. nakase@ort.med.osaka-u.ac.jp
Miyaji
T
T
Kuriyama
K
K
Tamai
N
N
Horiki
M
M
Tomita
T
T
Myoui
A
A
Shimada
K
K
Yoshikawa
H
H
D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002455:Cell Division / Q000235:genetics; D019902:Chondrocytes / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics; D053823:Hedgehog Proteins; D006801:Humans; D006984:Hypertrophy; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism; D015831:Osteochondroma / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D010010:Osteoclasts / Q000378:metabolism; D010012:Osteogenesis; D044162:Parathyroid Hormone-Related Protein; D000072079:Patched Receptors; D017689:Polydactyly / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics
D024042:Collagen Type I; D053823:Hedgehog Proteins; D008565:Membrane Proteins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D000072079:Patched Receptors; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D015534:Trans-Activators