title: On-line identification method in column liquid chromatography: UV resonance Raman spectroscopy.
abstract: Ultraviolet resonance Raman spectroscopy (RRS) is presented as a novel identification tool for conventional-size column liquid chromatography (LC). The on-line coupling was made using a standard Z-shaped flow cell. A continuous-wave frequency-doubled argon ion laser operating at a wavelength of 244 nm was used for excitation. "On-the-fly" resonance Raman spectra of four model compounds, fluorene, phenanthrene, fluoranthene, and pyrene, were recorded after a standard acetonitrile/water reversed-phase LC separation. When applying a large-volume-injection procedure (32 mL), detection limits were at the nanogram per milliliter level. The results indicate that UV-RRS gives detailed spectral information at an appropriate sensitivity level so that coupling with LC becomes feasible.
authors.affiliation: Department of Analytical Chemistry and Applied Spectroscopy, Free University, Amsterdam, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Dijkstra
authors.forename: C T
authors.initials: CT
authors.lastname: Martha
authors.forename: F
authors.initials: F
authors.lastname: Ariese
authors.forename: U A
authors.initials: UA
authors.lastname: Brinkman
authors.forename: C
authors.initials: C
authors.lastname: Gooijer