Inhibition of expression of ornithine decarboxylase by c-myc antisense oligonucleotide at the promotion stage of lung tumorigenesis in mice.
Regulation of ornithine decarboxylase (ODC) expression at a promotion stage of lung carcinogenesis is a key clue to suppress the cancer. In this study, we investigated that the ODC induction at the promotion stage of lung carcinogenesis in mice could be inhibited through the suppression of the expression of c-myc, a transcription factor for ODC. The treatment with c-myc antisense oligonucleotide decreased the carcinogen-elevated level of pulmonary ODC protein at the promotion stage, but the sense oligonucleotide had no influence on the level. Overall, it is possible that the induction of ODC in the carcinogenic process of lung is regulated at its transcriptional level.
Yano
T
T
Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan. yano@nih.go.jp
Yano
Y
Y
Horikawa
S
S
Satoh
H
H
Hagiwara
K
K
Ichikawa
T
T
Otani
S
S
D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D014158:Transcription, Genetic / Q000187:drug effects
D016376:Oligonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc; D009955:Ornithine Decarboxylase