Peroxidase activity of in vitro-selected 2'-amino RNAs.
Peroxidase activities of RNAs containing 2'-amino groups, which were selected as aptamers binding to N-methylmesoporphyrin IX, were investigated. Some clones promoted the oxidation reaction of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with hydrogen peroxide (H(2)O(2)) in the presence of iron(III)-protoporphyrin (hemin), whereas others did not. Each of them had a different substrate specificity. One of the active clones promoted the oxidation of o-dianisidine and beta-nicotinamide adenine dinucleotide reduced form (NADH) with H(2)O(2) 5 and 15 times faster than hemin only, respectively. On the other hand, one clone that was inactive on oxidation of ABTS exhibited the same level of activity on oxidation of o-dianisidine as that shown by the clone active on ABTS but no activity on NADH. By in vitro selection, we can produce various types of peroxidase-like non-natural RNAs.
Teramoto
N
N
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Ichinari
H
H
Kawazoe
N
N
Imanishi
Y
Y
Ito
Y
Y
D001483:Base Sequence; D052160:Benzothiazoles; D003962:Dianisidine / Q000378:metabolism; D006427:Hemin / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008652:Mesoporphyrins / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000378:metabolism*; D012313:RNA / Q000737:chemistry*; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000378:metabolism*
D052160:Benzothiazoles; D008652:Mesoporphyrins; D013451:Sulfonic Acids; D009243:NAD; C065420:N-methylmesoporphyrin IX; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D012313:RNA; D006427:Hemin; D006861:Hydrogen Peroxide; D009195:Peroxidase; D003962:Dianisidine