title: Crystallization and preliminary X-ray crystallographic analysis of native and selenomethionyl recombinant tabtoxin-resistance protein complexed with acetyl-coenzyme A.
abstract: Tabtoxin-resistance protein (TTR), an acetyltransferase from Pseudomonas syringae pv. tabaci, was overexpressed in Eschericha coli M15 and the TTR fusion protein complexed with acetyl-coenzyme A (AcCoA) was purified and crystallized. Diffraction data were collected to 3.0 A resolution in-house and the crystal was found to belong to space group P2(1), with unit-cell parameters a = 47.6, b = 66.6, c = 53.5 A, beta = 104.3 degrees. Furthermore, a selenomethionine (SeMet) TTR fusion protein derivative was overexpressed in the same expression system and its complex with AcCoA was purified in a reductive environment. The SeMet TTR derivative crystallized in two forms: the first was identical to that observed for native crystals and the second belonged to space group C2, with unit-cell parameters a = 101.7, b = 45.6, c = 84.2 A, beta = 105.8 degrees. Data from the P2(1) crystal form were collected in-house to 2.3 A resolution. Subsequently, three different wavelength data sets of the C2 crystal form to 1.55 A resolution were collected at the Advanced Photon Source at Argonne National Laboratory.
authors.affiliation: Laboratory of Structural Biology, School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: Z
authors.initials: Z
authors.lastname: Cao
authors.forename: Y
authors.initials: Y
authors.lastname: Shao
authors.forename: M
authors.initials: M
authors.lastname: Bartlam
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.forename: F
authors.initials: F
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: N
authors.initials: N
authors.lastname: Zhao
authors.forename: Z
authors.initials: Z
authors.lastname: Rao
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry*; D000123:Acetyltransferases / Q000737:chemistry*; D001426:Bacterial Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011549:Pseudomonas / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry; D012645:Selenomethionine / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000105:Acetyl Coenzyme A; D012645:Selenomethionine; D000123:Acetyltransferases; C441636:tabtoxin-resistance protein, Pseudomonas syringae