Influence of 7beta-hydroxycholesterol on glutathione status and nitric oxide production in macrophages: in vitro studies.
Incubation of murine peritoneal macrophages with 7beta-hydroxycholesterol (7beta-OH) for 24 hr led to dose-dependent reduction in cellular glutathione content as well as nitrite levels in the medium. Treatment with an inorganic form of selenium, sodium selenite which is a potent antioxidant, elevated the cellular glutathione levels and decreased nitrite levels. Our results suggest that 7beta-OH may exert its pro-atherogenic effect by inhibiting glutathione synthesis and nitric oxide production by macrophages present in the arterial wall and thus, impair the cellular antioxidant defense system.
Bansal
G
G
Department of Biophysics, Panjab University, Chandigarh, India.
Singh
U
U
Bansal
M P
MP
D000818:Animals; D001158:Arteries / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000378:metabolism; D012643:Selenium / Q000378:metabolism; D013997:Time Factors
D006888:Hydroxycholesterols; D009569:Nitric Oxide; C011724:cholest-5-en-3 beta,7 alpha-diol; D005979:Glutathione Peroxidase; D005978:Glutathione; D012643:Selenium