Hemin reconstitutes proton extrusion in an H(+)-ATPase-negative mutant of Lactococcus lactis.
H(+)-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H(+)-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
Blank
L M
LM
Department of Chemical Engineering, University of Queensland, 4072 Brisbane, Australia.
Koebmann
B J
BJ
Michelsen
O
O
Nielsen
L K
LK
Jensen
P R
PR
D002462:Cell Membrane / Q000378:metabolism; D006427:Hemin / Q000378:metabolism / Q000502:physiology*; D013294:Lactococcus lactis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
D006427:Hemin; D006180:Proton-Translocating ATPases