Influence of 2-bromopropane on reproductive system--short-term administration of 2-bromopropane inhibits ovulation in F344 rats.
The present study was performed to investigate the toxic effects of 2-bromopropane (2BP) on the female reproductive system. Female F344 rats were administered 2BP (500 or 1000 mg/kg, i.p.) at intervals of 2 or 3 days for 15-17 days. The body weights were measured and estrous stages were observed throughout the experimental period. Ovulation, organ weights, ovarian histology, and blood biochemistry were investigated on the terminal day of the experiment. Uterine weights in rats treated with 2BP were significantly lower than those in control animals. Body, liver, kidney, and adrenal weights in 2BP-treated rats showed no significant differences from control values. 2BP treatment prolonged estrous cycles and decreased the number of ovulated ova in spontaneous ovulation. In addition, histological examinations showed that the preovulatory follicles in the ovary were altered markedly in 2BP groups. These results show that even in short-term treatment, 2BP injured the ovary, particularly the preovulatory follicles. It appears that these damages of the preovulatory follicles induced by 2BP reduced the numbers of spontaneously ovulated ova in female F344 rats.
Sekiguchi
S
S
Division of Health Effects Research, National Institute of Industrial Health, Kawasaki, Japan.
Asano
G
G
Suda
M
M
Honma
T
T
D000681:Amylases / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D004305:Dose-Response Relationship, Drug; D030762:Estrous Cycle / Q000187:drug effects*; D005260:Female; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000473:pathology*; D010060:Ovulation / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D014599:Uterus / Q000473:pathology
D006842:Hydrocarbons, Brominated; D009153:Mutagens; D002784:Cholesterol; D000681:Amylases; C103736:2-bromopropane