Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA.
In Escherichia coli, an interaction between the replication initiator DnaA and the sliding clamp protein, the beta subunit (DnaN) of DNA polymerase III, is required to regulate the chromosomal replication cycle. We report here that colony formation by, and cell division of, the temperature (42 degrees C)-sensitive dnaN59 mutant are inhibited at 34-35 degrees C when DnaA is moderately (4-to 8-fold ) overexpressed, although chromosomal replication and the beta subunit-dependent regulation of DnaA activity are not significantly inhibited. Immunoblotting analysis revealed that the beta subunit is abundant (present at a level of about 5000 dimers per cell) at 34 degrees C, and its concentration per unit cell volume was practically unaffected in the dnaN59 mutant by the overexpression of DnaA. The dnaN mutant cells that overexpress DnaA become filamentous at 34 degrees C via an sfiA-independent pathway, different from that activated by the SOS response. This filamentation is accompanied by inhibition of nucleoid partition and FtsZ ring formation. In the dnaN59 mutant, oversupply of DnaA may disturb the coordinated action of cell cycle-regulating molecules, thus leading to the inhibition of these events.
Kawakami
H
H
Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Iwura
T
T
Takata
M
M
Sekimizu
K
K
Hiraga
S
S
Katayama
T
T
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D003598:Cytoskeletal Proteins; D004258:DNA Polymerase III / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D015151:Immunoblotting; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D021122:Protein Subunits; D013696:Temperature
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; C071525:FtsZ protein, Bacteria; D021122:Protein Subunits; C023140:DNA replicase; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase