Protein adsorption on novel acrylamido-based polymeric ion-exchangers. III. Salt concentration effects and elution behavior.
The effect of salt concentration on the adsorption and desorption of BSA has been determined for a polymeric anion-exchanger based on acrylamido monomers. The material investigated possesses a high adsorption capacity at low salt concentration and the bound protein can be recovered quantitatively at high salt concentrations. The effects of salt on adsorption and desorption rates were evaluated from batch and shallow-bed experiments, and a model was developed to describe the data quantitatively. The adsorption capacity decreases as the salt concentration is increased, but both adsorption and desorption rates increase at higher salt concentrations. The predictability of the behavior of columns packed with this material was examined by comparing model predictions and experimental results obtained in laboratory columns. In general, a good agreement was obtained between predicted and experimental breakthrough and elution profiles, especially in shorter columns. Thus, the model allows a prediction of the effects of column length, mobile phase flow-rate, protein feed concentration, and salt concentration on dynamic capacity, productivity, and on the concentration of product fractions.
Hunter
A K
AK
Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442, USA.
Carta
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D020106:Acrylamide / Q000737:chemistry*; D000327:Adsorption; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D007475:Ion Exchange Resins / Q000737:chemistry*; D007700:Kinetics; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D007475:Ion Exchange Resins; D011506:Proteins; D012492:Salts; D020106:Acrylamide