Human FATE is a novel X-linked gene expressed in fetal and adult testis.
Previously, we identified a partial cDNA sequence of a novel human transcript, designated fetal and adult testis expressed transcript (FATE). FATE is testis-specific in fetal life and co-expressed with SRY in a 7 weeks old fetal testis, suggesting a function in early testicular differentiation. Herein, full-length cDNA clones of human and porcine FATE were isolated and the gene structure and promoter region of the human FATE gene was characterized. The human FATE gene, which maps to Xq28, consists of five exons spanning approximately 7 kb of genomic DNA. Examination of 1 kb of the FATE promoter region revealed the presence of a putative steroidogenic factor 1 (SF-1) binding site at position -79 to -71 upstream of the transcription start site. We propose that FATE might represent a novel target gene of SF-1 in human testicular differentiation and/or germ cell development.
Olesen
C
C
Department of Medical Genetics, Wilhelm Johannsen Center for Functional Genome Research, Institute of Medical Biochemistry and Genetics, University of Copenhagen, DK-2200 Copenhagen N., Denmark. christian@medgen.ku.dk
Larsen
N J
NJ
Byskov
A G
AG
Harboe
T L
TL
Tommerup
N
N
D000328:Adult; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D005333:Fetus; D051717:Fushi Tarazu Transcription Factors; D024282:Genes, sry; D008040:Genetic Linkage; D018398:Homeodomain Proteins; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D013552:Swine; D013737:Testis / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D014960:X Chromosome / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C449758:FATE1 protein, human; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; C515057:NR5A1 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors