title: Potential use of Amplicor PCR kit in diagnosing pulmonary tuberculosis from gastric aspirate.
abstract: Polymerase chain reaction (PCR) detection of mycobacteria from gastric aspirate for the diagnosis of tuberculosis is not fully evaluated up to now. A total of 116 gastric aspirate specimens were collected from patients with suspected pulmonary tuberculosis. The breakdown of diagnosis was 67 pulmonary tuberculosis, 16 nontuberculous mycobacterial infection, 5 extra pulmonary tuberculosis, and 28 other lung diseases. The conventional methods were shown to have a sensitivity of 47.8% and a specificity of 79.6%; on the other hand, Amplicor had 34.9% and 97.0%, respectively. The Amplicor provided a more rapid and specific method for diagnosing tuberculosis and was more useful than the conventional.
authors.affiliation: Department of Respiratory Medicine, Tokyo National Hospital, 3-1-1 Takeoka, Kiyose, Tokyo, 204-0023, Japan. mitarai@jata.or.jp
authors.forename: S
authors.initials: S
authors.lastname: Mitarai
authors.forename: S
authors.initials: S
authors.lastname: Tanoue
authors.forename: C
authors.initials: C
authors.lastname: Sugita
authors.forename: E
authors.initials: E
authors.lastname: Sugihara
authors.forename: A
authors.initials: A
authors.lastname: Tamura
authors.forename: Y
authors.initials: Y
authors.lastname: Nagono
authors.forename: M
authors.initials: M
authors.lastname: Tsuboi
authors.forename: N
authors.initials: N
authors.lastname: Nagayama
authors.forename: A
authors.initials: A
authors.lastname: Kurashima
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: H
authors.initials: H
authors.lastname: Shishido
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005750:Gastric Juice / Q000382:microbiology; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D011933:Reagent Kits, Diagnostic