Spatial temperature gradient capillary electrophoresis for DNA mutation detection.
A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.
Zhu
L
L
Department of Chemistry National University of Singapore, Republic of Singapore.
Lee
H K
HK
Lin
B
B
Yeung
E S
ES
D004247:DNA / Q000032:analysis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D017354:Point Mutation; D013696:Temperature; D013816:Thermodynamics
D007202:Indicators and Reagents; D009692:Nucleic Acid Heteroduplexes; D004247:DNA