Photodynamic effect on cancer cells influenced by electromagnetic fields.
The synergism of low-frequency electromagnetic field treatment and photodynamic effect on killing of human cancer cells is presented. The weak pulsating electromagnetic field (PEMF) generated by Helmholtz coils in the mT range influences the permeability of cell membranes for photosensitizers. Several types of sensitizers were excited by visible light during incorporation without and with two kinds of PEMF treatment. In the first part suitable photosensitizers were selected in the absorption range between 400 and 700 nm against human myeloid leukaemia K562 and human histiocytic lymphoma U937 cells by treatment of PEMF consisting of rectangular pulse groups. In the second part amplitude and frequency dependencies were measured using sinuous PEMF and white light with the result that after 12 min the PEMF treatment enhanced photodynamic effectivity by more than 40% over the control value. Taking into account the influence of many parameters, an additional optimization will be possible by photodynamic PEMF synergism for an increased drug delivery in general.
Pang
L
L
Institute of Physics, Nankai University, Nankai, PR China.
Baciu
C
C
Traitcheva
N
N
Berg
H
H
D002463:Cell Membrane Permeability / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006801:Humans; D007211:Indoles / Q000633:toxicity; D054833:Isoindoles; D020014:K562 Cells; D009942:Organometallic Compounds / Q000633:toxicity; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000633:toxicity*; D011524:Protoporphyrins / Q000633:toxicity; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C028025:protoporphyrin IX