Gene transfer using human polyomavirus BK virus-like particles expressed in insect cells.
The major structural protein (VP1) of the BK polyomavirus (BKV) was expressed in the recombinant baculovirus expression system. Recombinant BKV VP1 was shown to self-assemble into virus-like particles (VLPs) with a diameter of 45-50 nm. As for other polyomaviruses, BKV VP1 has the capacity to bind to exogenous DNA. Furthermore, the potential of BKV VP1 VLPs was investigated for gene transfer into COS-7 cells using three methods for the formation of pseudo-virions: disassembly/reassembly, osmotic shock and direct interaction between VLPs and reporter plasmid DNA. The latter method was shown to be the most efficient when using linearized plasmid. Gene transfer efficiency with BKV pseudo-virions was of the same order as that observed with human papillomavirus type 16 L1 protein VLPs. In addition, it is demonstrated that cellular entry of BKV pseudo-virions is dependent on cell surface sialic acid.
Touzé
Antoine
A
Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France1.
Bousarghin
Latifa
L
Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France1.
Ster
Céline
C
Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France1.
Combita
Alba-Lucia
AL
Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France1.
Roingeard
Philippe
P
Laboratoires de Biologie Cellulaire et Virologie, EA 2639, Analyse Structurale des Antigènes, IFR Transposons et Virus, Faculté de Médecine, 2 bis Boulevard Tonnellé, 37032 Tours cedex, France2.
Coursaget
Pierre
P
Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France1.
D000818:Animals; D001739:BK Virus / Q000235:genetics*; D016367:Baculoviridae / Q000235:genetics; D019556:COS Cells / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007313:Insecta; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011485:Protein Binding; D011995:Recombination, Genetic; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism; D019065:Virus Assembly
D015678:Viral Structural Proteins; D004247:DNA; D019158:N-Acetylneuraminic Acid