Autologous platelet-rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel.
OBJECTIVE
We compared three methods of isolating platelet-rich plasma (PRP) using the Haemonetics Cell Saver 5 and one method of isolating PRP by plateletpheresis using the Haemonetics MCS+. PRP contains both platelets and fibrinogen, which are used in the preparation of haemostatic agents.


METHODS
When the Haemonetics Cell Saver 5 was used, 500 ml of blood from each of 30 normal volunteer donors was collected into 70 ml of citrate-phosphate-dextrose (CPD) anticoagulant. In a further 14 normal volunteers, the Haemonetics MCS+ was used to isolate PRP by plateletpheresis using an acid citrate dextrose (ACD) to blood ratio of 1 : 9. In a separate study, CPD-anticoagulated whole blood from another 30 volunteers was used for measurement of fibrinogen levels in the plasma and cryoprecipitate.


RESULTS
A larger volume of PRP can be collected using the Haemonetics Cell Saver 5 than by using the Haemonetics MCS+. The platelet concentration and the total number of platelets were higher in the PRP isolated using the Haemonetics MCS+ than in the PRP isolated by the three methods used with the Haemonetics Cell Saver 5, with differences in platelet concentration and PRP volume among the four methods. The mean fibrinogen level in the plasma was 253 mg % +/- 47 (SD) and in the cryoprecipitate was 1085 mg % +/- 304 (SD).


CONCLUSIONS
The most appropriate method of PRP isolation for preparation of platelet gel is dependent upon the specific surgical procedure to be undertaken and the patient's needs.
O'Neill
E M
EM
American Red Cross Blood Services, New England Region, Dedham, MA, USA.
Zalewski
W M
WM
Eaton
L J
LJ
Popovsky
M A
MA
Pivacek
L E
LE
Ragno
G
G
Valeri
C R
CR
D001804:Blood Transfusion, Autologous / Q000295:instrumentation / Q000379:methods*; D005340:Fibrinogen / Q000032:analysis; D006488:Hemostasis, Surgical; D006490:Hemostatics / Q000302:isolation & purification; D006801:Humans; D010956:Plasmapheresis / Q000295:instrumentation*; D010976:Platelet Count; D017713:Platelet Transfusion / Q000379:methods / Q000592:standards; D013514:Surgical Procedures, Operative
D006490:Hemostatics; D005340:Fibrinogen