[Retroviral-mediated transcriptional regulatory elements of alpha-fetoprotein gene directed specific expression of interleukin-2 in murine hepatoma].
OBJECTIVE
To target expression of interleukin-2 in alpha-fetoprotein (AFP) positive hepatocellular carcinoma.


METHODS
A retroviral vector pDOR-mAFP-IL2 was constructed by coupling the transcriptional regulatory elements (TREs) of murine AFP gene with interleukin-2 cDNA in a retroviral vector.


RESULTS
Subsequent to retroviral infection, expression of IL-2 could be detected in an AFP positive murine hepatoma cell line Hepa1-6 but not in psi 2, an AFP negative murine fibroblast cell line.


CONCLUSIONS
Our results indicate that specific expression of IL-2 in hepatoma cell can be achieved by using of AFP TREs to control the expression of IL-2, and the retroviral vector which we have constructed may be used for further experimental studies on in vivo gene therapy.
Ma
Q
Q
Cancer Institute, CAMS and PUMC, Beijing 100021.
Wu
Y
Y
Yu
L
L
Zhang
Y
Y
D000818:Animals; D005809:Genes, Regulator; D015316:Genetic Therapy; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics*
D007376:Interleukin-2; D000509:alpha-Fetoproteins