Isolation of human scFv antibody fragments against ABO blood group antigens from a phage display library.
OBJECTIVE
Phage display has proven very useful for the isolation of antibodies against a number of antigens. We used this technology to isolate scFv antibody fragments against A and B red blood cell antigens.


METHODS
Phages from a phage display library were selected using unmodified red blood cells as a source of antigen. Bound phages were absorbed onto cells lacking the antigen of interest and used to infect Escherichia coli cells. Phages were rescued and assayed for specificity by enzyme-linked immunosorbent assay (ELISA).


RESULTS
After several rounds of panning and subtraction on red blood cells, one anti-A and one anti-B human scFv antibody fragments were isolated.


CONCLUSIONS
Isolation of anti-A and anti-B scFv antibody fragments on whole cells is an alternative method of obtaining antibodies against native cell-surface antigens.
Santos-Esteban
E
E
Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas del I. P. N., Prol. de Carpio y Plan de Ayala, CP 11340, México D.F., México.
Curiel-Quesada
E
E
D000017:ABO Blood-Group System / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology; D017104:Bacteriophage M13; D015723:Gene Library; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D008722:Methods
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D007128:Immunoglobulin Fragments; C055966:immunoglobulin Fv