[Model of human nasal gland cells cultured in collagen gels].
OBJECTIVE
To establish a culture model of human nasal gland (HNG) cells in collagen gels.


METHODS
The HNG cells were cultured in collagen gels.


RESULTS
The HNG cells cultured in collagen gels showed 3-D outgrowth. After one week culture, the HNG cells developed ductal projections, the sections of collagen gel culture examined by light microscopy showed cross sectional area of tubules with a lumen containing secretory products. The cells contained numerous secretory granules. Electron microscopy showed 3-D cultured HNG cells forming a well defined lumen inside the tubular formation. These cells were polarized and interconnected by junctional complexes and possessed extensive microvilli along all their luminal border. Secretory products in the lumen and the cells located close to the luminary surface were intensely stained blue by AB-PAS staining. Immunohistochemical staining of cells labeled with a rabbit antiserum against human lysozyme showed 90% positivity.


CONCLUSIONS
These data indicated that HNG cells altered their phenotypes when cultured in vitro and changed from differentiated serous and mucous cells toward intermediate seromucous cells.
Guo
Y
Y
Third Clinical College of Bethune University of Medical Sciences, Changchun 130031.
Yang
Z
Z
Bu
G
G
D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology* / Q000648:ultrastructure; D010641:Phenotype
D003470:Culture Media; D003094:Collagen