title: Demonstration of Epstein-Barr virus in odontogenic and nonodontogenic tumors by the polymerase chain reaction (PCR).
abstract: BACKGROUND
  Although considerable insight has been gamed into Epstein-Barr virus (EBV) as an important etiologic factor in various tumors, virtually little is known about the relationship between EBV genes and oral tumors.
  
  
  METHODS
  Thirty-two cases of nonodontogenic tumor (16 squamous cell carcinomas, 11 salivary gland tumors, 1 malignant lymphoma, 1 spindle cell sarcoma, 1 osteogenic sarcoma, 1 malignant fibrous histiocytoma and 1 verrucous carcinoma), 17 cases of odontogenic tumor (17 ameloblastomas, the most important and common type of odontogenic tumor) and 12 cases of normal oral tissue (8 normal gingival tissues and other oral mucosa) were examined for the presence of EBV-DNA, with primers specific for the BamW, BNRF1, BMLF1, BamC, IR3, BMRF1, EBNA-2A BamhY, and EBNA-2B BamhY region of the EBV genome by the polymerase chain reaction (PCR).
  
  
  RESULTS
  Fifty-three percent (17/32) of nonodontogenic tumors, forty-eight percent (8/17) of ameloblastomas, and ninety-two percent (11/12) of normal oral tissues were positive for EBV-DNA. Of the EBV-DNA, BMLF1 demonstrated the strongest reactivity in the nonodontogenic tumors, and BamC demonstrated the strongest reactivity in the ameloblastomas and normal oral mucosae.
  
  
  CONCLUSIONS
  Taken into account with the expression of different EBV genes in odontogenic and nonodontogenic tumors, these findings suggest that even though odontogenic tumors and nonodontogenic tumors are relatively unique, the appearance of different EBV genes seems to suggest the complicated roles that the EBV genes play.
authors.affiliation: Department of Oral Pathology, College of Dentistry, Chosun University, Kwang-ju, Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Jang
authors.forename: J O
authors.initials: JO
authors.lastname: Cho
authors.forename: C Y
authors.initials: CY
authors.lastname: Yoon
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: J C
authors.initials: JC
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000821:virology; D009062:Mouth Neoplasms / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral