Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.
Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.
Maksimova
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EE
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russia.
Popova
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LY
D000222:Adaptation, Physiological; D003264:Containment of Biohazards; D017753:Ecosystem; D004926:Escherichia coli; D005618:Fresh Water / Q000382:microbiology; D015870:Gene Expression; D008163:Luminescent Measurements; D030781:Organisms, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D010776:Photobacterium; D010957:Plasmids; D018570:Risk Assessment; D014871:Water Microbiology