title: Crystallization and preliminary X-ray analysis of maltose O-acetyltransferase.
abstract: Maltose O-acetyltransferase (Mac) is a member of the hexapeptide-repeat family of enzymes, which contains proteins with left-handed parallel beta-helix architecture forming homotrimers. Diffraction data for four well diffracting crystal forms were collected. Crystal form I diffracted beyond 1.53 A resolution but was perfectly merohedrally twinned with an apparent space group P622. Crystal forms II and III (space groups R3 and C2, respectively) could be obtained under very similar conditions by adjusting the buffer pH differently. Crystal forms II and III had several monomers in the asymmetric unit and were difficult to derivatize. However, during soaking with trimethyl lead acetate, the form III crystals dissolved and crystals with a different habit and space group grew in their place (form IV). In three of the crystal forms, a ladder of peaks was visible in the native Patterson maps along the c axis. These peaks were interpreted as corresponding to the vectors between the beta-strands in the turns of the beta-helix. Crystal form IV is suitable for structure determination of Mac exploiting the anomalous scattering of lead.
authors.affiliation: Centre for Crystallographic Studies, Chemical Institute, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Lo Leggio
authors.forename: F
authors.initials: F
authors.lastname: Dal Degan
authors.forename: P
authors.initials: P
authors.lastname: Poulsen
authors.forename: S O
authors.initials: SO
authors.lastname: Sørensen
authors.forename: K
authors.initials: K
authors.lastname: Harlow
authors.forename: P
authors.initials: P
authors.lastname: Harris
authors.forename: S
authors.initials: S
authors.lastname: Larsen
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D011108:Polymers; D000123:Acetyltransferases; C033939:maltose acetyltransferase