title: An mRNA degrading complex in Rhodobacter capsulatus.
abstract: An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
authors.affiliation: Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Jäger
authors.forename: O
authors.initials: O
authors.lastname: Fuhrmann
authors.forename: C
authors.initials: C
authors.lastname: Heck
authors.forename: M
authors.initials: M
authors.lastname: Hebermehl
authors.forename: E
authors.initials: E
authors.lastname: Schiltz
authors.forename: R
authors.initials: R
authors.lastname: Rauhut
authors.forename: G
authors.initials: G
authors.lastname: Klug
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D002458:Cell Fractionation; D002498:Centrifugation / Q000379:methods; D004722:Endoribonucleases / Q000276:immunology / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D020365:RNA Helicases / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein
chemical_list: D000906:Antibodies; D046911:Macromolecular Substances; D012333:RNA, Messenger; D004722:Endoribonucleases; C022537:ribonuclease E; D020365:RNA Helicases