Fluorescence spectral study of interaction of water-soluble metal complexes of Schiff-base and DNA.
The fluorescence spectral characteristics and the interaction of several water-soluble metal complexes of Schiff-base with DNA are described. Among the complexes tested, Mn-Schiff-base bound to DNA showed a marked decrease in the fluorescence intensity with a blue shift of the excitation and emission peaks. Some hypochromism in the UV absorption spectra was also observed. KI quenching and competitive binding to DNA between Mn-Schiff-base and ethidium bromide (EB) were studied in connection with other experimental observations to show that the interactive model between Mn-Schiff-base and DNA is an intercalative one. The pH and salt effect on the fluorescence properties was also investigated. The linear relationship between F/F0 and the concentration of calf thymus DNA covers 3.0 x 10(-6)-2 x 10(-4) mol L-1, which can be utilized for determining traces of calf thymus DNA with a detection limit of 8.0 x 10(-7) mol L-1 in base pairs.
Liu
G D
GD
College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, P. R. China.
Liao
J P
JP
Huang
S S
SS
Shen
G L
GL
Yu
R Q
RQ
D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008670:Metals / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry*; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014867:Water
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008670:Metals; D012545:Schiff Bases; D014867:Water; D004247:DNA