title: Defective infection of bovine herpesvirus 1 in non-permissive murine cells.
abstract: The defective growth of bovine herpesvirus I (BHV-1) was analyzed in non-permissive murine embryo fibroblast, BALB/3T3 A31-1-1 (A31) cells. BHV-1 was able to attach and penetrate into A31 cells at similar levels that were seen in semi-permissive cells. Once penetrated into A31 cells, BHV-1 was efficiently transported to nuclei, but the onset of expression of immediate early (IE) protein and viral DNA replication was not observed. These data suggest that the viral replication of BHV-1 in A31 cells is arrested at the point prior to the expression of IE proteins.
authors.affiliation: Department of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamichi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D006224:Cricetinae; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D019084:Fluorescent Antibody Technique, Indirect; D006566:Herpesviridae Infections / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development* / Q000502:physiology; D051379:Mice; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C083786:immediate early protein BICP4, bovine herpesvirus 1