An improved method to illuminate and record a phytase assay in SDS-PAGE zymogram gels.
Experimentation at the Lethbridge Research Center in Alberta, Canada using cross-polarized transmitted light to photographically record a staining technique on zymograms has proved to be successful with both color and black and white films. It has been possible to obtain the desired visible contrast without compromising the intensity of the enzyme activity bands. Increasing numbers of such PAGE gels are being submitted for photographic recording when it is believed that the image will be used for records, publication, scientific posters or AV presentations.
Sinclair
K G
KG
Agriculture and Agri-Food Canada's Research Center, Lethbridge, Alberta, Canada.
Selinger
L B
LB
D'Silva
C G
CG
Bae
H D
HD
Yanke
L J
LJ
Cheng
K J
KJ
D010832:6-Phytase / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007124:Immunoenzyme Techniques; D008859:Microscopy, Polarization / Q000295:instrumentation; D016624:Negative Staining; D012680:Sensitivity and Specificity
D010832:6-Phytase