title: Nanoliter capillary electrochromatography columns based on collocated monolithic support structures molded in poly(dimethyl siloxane).
abstract: Following current trends in miniaturization of analytical chemistry, an inexpensive disposable analytical tool in the form of a liquid chromatography column fabricated on a poly(dimethyl siloxane) (PDMS) chip was created. Ease of fabricating the chromatography column was demonstrated by molding collocated monolithic support structures (COMOSS) directly in the column. Positive photo-resist, SPR 220, was used to create column structures on a negative relief master providing final channel dimensions of 2.7-5.2 microm wide by 10.0 microm deep, while monolithic dimensions were 9.8 x 9.8 x 10.0 microm - 12.3 x 12.3 x 10.0 microm. The ability to separate biological samples such as peptides from a tryptic digest of fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) was shown. Separations in capillary electrochromatographic (CEC) mode were performed yielding column efficiencies of 4.0 x 10(5) plates/m.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Slentz
authors.forename: N A
authors.initials: NA
authors.lastname: Penner
authors.forename: E
authors.initials: E
authors.lastname: Lugowska
authors.forename: F
authors.initials: F
authors.lastname: Regnier
mesh_terms: D000818:Animals; D002197:Capillary Action; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000302:isolation & purification*; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D012828:Silicones; D013050:Spectrometry, Fluorescence
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D007202:Indicators and Reagents; D012828:Silicones; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; C013830:baysilon; D016650:Fluorescein-5-isothiocyanate