Sea urchin goosecoid function links fate specification along the animal-vegetal and oral-aboral embryonic axes.
We have identified a single homolog of goosecoid, SpGsc, that regulates cell fates along both the animal-vegetal and oral-aboral axes of sea urchin embryos. SpGsc mRNA is expressed briefly in presumptive mesenchyme cells of the approximately 200-cell blastula and, beginning at about the same time, accumulates in the presumptive oral ectoderm through pluteus stage. Loss-of-function assays with morpholine-substituted antisense oligonucleotides show that SpGsc is required for endoderm and pigment cell differentiation and for gastrulation. These experiments and gain-of-function tests by mRNA injection show that SpGsc is a repressor that antagonizes aboral ectoderm fate specification and promotes oral ectoderm differentiation. We show that SpGsc competes for binding to specific cis elements with SpOtx, a ubiquitous transcription activator that promotes aboral ectoderm differentiation. Moreover, SpGsc represses transcription in vivo from an artificial promoter driven by SpOtx. As SpOtx appears long before SpGsc transcription is activated, we propose that SpGsc diverts ectoderm towards oral fate by repressing SpOtx target genes. Based on the SpGsc-SpOtx example and other available data, we propose that ectoderm is first specified as aboral by broadly expressed activators, including SpOtx, and that the oral region is subsequently respecified by the action of negative regulators, including SpGsc. Accumulation of SpGsc in oral ectoderm depends on cell-cell interactions initiated by nuclear beta-catenin function, which is known to be required for specification of vegetal tissues, because transcripts are undetectable in dissociated or in cadherin mRNA-injected embryos. This is the first identified molecular mechanism underlying the known dependence of oral-aboral ectoderm polarity on intercellular signaling.
Angerer
L M
LM
Department of Biology, University of Rochester, Rochester, NY 14627, USA. langerer@mail.rochester.edu
Oleksyn
D W
DW
Levine
A M
AM
Li
X
X
Klein
W H
WH
Angerer
R C
RC
D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004064:Digestive System / Q000196:embryology; D004707:Endoderm / Q000166:cytology; D005775:Gastrula / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D051956:Goosecoid Protein; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009055:Mouth / Q000196:embryology; D051857:Otx Transcription Factors; D010858:Pigmentation; D011485:Protein Binding; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012617:Sea Urchins / Q000196:embryology*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015534:Trans-Activators; D014157:Transcription Factors; D014158:Transcription, Genetic; D051176:beta Catenin
D003598:Cytoskeletal Proteins; D051956:Goosecoid Protein; D018398:Homeodomain Proteins; D051857:Otx Transcription Factors; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin