[Induction of potent antitumor immune response of leukemia antigen-pulsed dendritic cells by IL-2 gene modification].
OBJECTIVE
To investigate whether adenovirus-mediated IL-2 gene modification can improve antitumor immunity of the tumor antigen-pulsed dendritic cells(DC) in vivo.


METHODS
DC were generated from bone marrow, then, pulsed with FBL-3 erythroleukemia cell lysates and transfected with IL-2 gene simultaneously. IL-2 secretion and T cell stimulating activity of the DC were detected. The number and cell subsets of draining lymph nodes, cytotoxic lymphocyte(CTL) activity, and the increase of protective effect for the mice s.c. vaccinated with DC were observed.


RESULTS
IL-2 gene-modified, antigen-pulsed DC could secrete high level of IL-2 in vitro, stimulate proliferation of syngeneic T cells markedly. DC vaccine could increase the number of draining lymph node and induce CTL activity, which directed to FBL-3, more significantly. After the DC vaccination, the survival time of the mice challenged with wild-type FBL-3 cells were prolonged.


CONCLUSIONS
Antitumor immune response could be induced more potently by vaccination with IL-2 gene modified, antigen-pulsed DC.
Tang
H
H
Department of Immunology, Second Military Medical University, Shanghai 200433.
Cao
X
X
Zhu
X
X
D000818:Animals; D002455:Cell Division; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D007376:Interleukin-2 / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology* / Q000628:therapy; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D007376:Interleukin-2