title: In vitro approach to the chemical drive of breathing.
abstract: Since its introduction two decades ago, the isolated brain stem-spinal cord preparation of neonatal rodents has been the preferred method used to reveal the mystery underlying the genesis of the respiratory rhythm. Little research using this in vitro approach has focused on the study of the central respiratory chemosensitivity. Some unexpected findings obtained with the brain stem-spinal cord preparation have added new questions that challenge our previous theoretic framework. Some of these findings are addressed here.
authors.affiliation: Laboratorio de Sistemas Neurales, Depto. Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Avda. Bdo. O'Higgins 3363, Casilla 40 Correo 33, Santiago, Chile. jeugenin@lauca.usach.cl
authors.forename: J
authors.initials: J
authors.lastname: Eugenín
authors.forename: I
authors.initials: I
authors.lastname: Llona
authors.forename: C D
authors.initials: CD
authors.lastname: Infante
authors.forename: E
authors.initials: E
authors.lastname: Ampuero
mesh_terms: D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D001933:Brain Stem / Q000737:chemistry / Q000502:physiology; D002628:Chemoreceptor Cells / Q000737:chemistry* / Q000502:physiology; D008954:Models, Biological; D009893:Opossums; D012119:Respiration; D012377:Rodentia; D013116:Spinal Cord / Q000737:chemistry* / Q000502:physiology
chemical_list: D000109:Acetylcholine