The Platelia Aspergillus ELISA in diagnosis of invasive pulmonary aspergilosis (IPA).
The sensitivity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was evaluated with 66 serum samples and 113 specimens of the respiratory tract obtained from 52 patients with pulmonary diseases. The patients were divided into five groups: proven invasive pulmonary aspergillosis (IPA) (five patients), probable IPA (seven patients), Aspergillus colonization (eight patients) or unlikely Aspergillus infection (27 patients). Another five patients with doubtful diagnostic test results are discussed in detail. The results of the Platelia Aspergillus ELISA (Sanofi Pasteur, Freiburg, Germany) in testing specimens of the respiratory tract were 90% sensitivity in proven (serum 38%), 60% in probable (serum 37%) and 71% in Aspergillus colonization (serum 0%). Furthermore, 85% of the Aspergillus spp. from positive cultures of specimens of the respiratory tract were also detected in the ELISA. A total of 57% of the culture negative specimens of patients with a least one positive culture or proven aspergillosis in a series of specimens were positive in the ELISA.
Siemann
M
M
Pathologisch-Bakteriologisches Institut, Städtisches Krankrenhaus, Kiel, Germany.
Koch-Dörfler
M
M
D000328:Adult; D000368:Aged; D000946:Antigens, Fungal / Q000097:blood; D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology; D001230:Aspergillus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D008351:Mannans / Q000276:immunology; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose