[Effect of activated donor-type NK cells on allogeneic bone marrow transplantation in mice].
OBJECTIVE
To study the effect of activated donor-type NK cells on allogeneic bone marrow transplantation(BMT).


METHODS
Four hours before or 72 hours after transfusing bone marrow cells(containing T cells), the activated donor-type NK cells were injected into tumor-bearing mice conditioned by irradiation. The effect of these NK cells were assessed by CFU-S, CFU-GM, survival, body weight, histopathology and lung metastases in the recipients.


RESULTS
The transfusion of activated donor-type NK cells markedly increased CFU-S and CFU-GM numbers. When these NK cells were transfused 4 hours before BMT, they augmented GVT yet inhibited GVHD as evidenced by increases in survival duration and body weight, reduction in grade of histopathological changes and decrease in lung metastases. However, when activated donor-type NK cells were transfused 72 hours after BMT, they exacerbated GVHD.


CONCLUSIONS
The activated donor-type NK cells could prevent GVHD and produce significant GVT effects and also resulted in greater hematopoietic reconstitution if they were at the initial stage of allogeneic BMT in mice. Thereafter, the activated donor-type NK cells were detrimental and could exacerbate the subsequent GVHD, these results also demonstrated that GVT and GVHD could be dissociable phenomena.
Sun
K
K
Department of Hematology, Affiliated Hospital, Shandong Medical University, Jinan 250012.
Zhang
M
M
Tian
Z
Z
D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control*; D020350:Graft vs Tumor Effect; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D014184:Transplantation, Homologous