title: Hydrolysis of linear DNA duplex catalyzed by Co(III) complex of cyclen attached to polystyrene.
abstract: To design artificial restriction enzymes, synthetic catalytic centers that effectively hydrolyze linear double-stranded polydeoxyribonucleotides are needed. The Co(III) complex of cyclen (CoCyc) attached to polystyrene derivatives hydrolyzes linearized pUC18 DNA with half-lives as short as 30 min at 25 degrees C. The catalytic activity of CoCyc is enhanced by >150 times on attachment to the resin.
authors.affiliation: Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, Republic of Korea.
authors.forename: C S
authors.initials: CS
authors.lastname: Jeung
authors.forename: J B
authors.initials: JB
authors.lastname: Song
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Suh
mesh_terms: D002384:Catalysis; D003035:Cobalt / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D011137:Polystyrenes; C442399:cyclencobalt(III); D003035:Cobalt; D004247:DNA