Possible origin of extrachromosomal DNA from human lymphocytes following in vitro treatment by phytohemagglutinin.
Lymphocytes respond to chemical or pathological challenges by synthesizing episomes or extrachromosomal DNA (eDNA). Phytohemagglutinin (PHA) is a mitogen for T lymphocytes and can stimulate the increased production of episomes as well as the release of eDNA, interleukin-2, and other factors. However, the origin of this eDNA is largely unknown. To confirm the observation on the localization of an antibody directed against a 23-kb segment of bull acrosomal DNA in cultured human lymphocytes, we performed analyses by fluorescence in situ hybridization (FISH) using the same DNA as a molecular probe.
Chen
J S
JS
Centro per lo Studio delle Cellule Germinale, Consiglio Nazionale delle Ricerche, Siena, Italy. chen@unisi.it
Chen
M G
MG
D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology*
D010835:Phytohemagglutinins; D004247:DNA