title: Immunocytochemical identification of GtH1 and GtH2 cells during the temperature-sensitive period for sex determination in pejerrey, Odontesthes bonariensis.
abstract: The ontogeny of gonadotropin 1 (GtH1) and 2 (GtH2) cells and its possible link to gonadal sex differentiation were studied in pejerrey, Odontesthes bonariensis, by immunocytochemistry using anti-chum salmon beta-GtH1 and beta-GtH2 antisera. In adults, GtH1 cells were found in the proximalis pars distalls (PPD) close to the neurohypophysis, whereas GtH2 cells were identified surrounding GtH1 cells, at the external layer of the PPD and in the pars intermedia. Essentially the same distribution was observed in larvae. In pejerrey, the phenotypic sex is governed by the temperature during the critical period of sex determination (temperature-dependent sex determination, TSD). Female proportions vary gradually from 100% at 15-19 degrees to 0% at 29 degrees, and the critical time of TSD has been estimated to be 3-5, 2-4, and 1-4 weeks after hatching at 17, 19, and 27 degrees, respectively. Thus, the expression of both GtHs was examined weekly in larvae reared from hatching to week 11, at 17, 24, and 29 degrees. The proportion of females at 17, 24, and 29 degrees was, 100%, 78%, and 0%, respectively. Histological ovarian differentiation was noticed at 7 and 4 weeks at 17 and 24 degrees, whereas testicular differentiation occurred at 7 and 4 weeks at 24 and 29 degrees. GtH1 cells were first observed at week 3 at 29 degrees and at week 4 at 17 and 24 degrees. These cells increased in number until week 4 and then decreased, disappearing after week 6 at all temperature regimes. GtH2 cells appeared at week 2 at 24 and 29 degrees and at week 3 at 17 degrees. GtH2 cell number increased until week 3 at 29 degrees and until week 4 at 17 and 24 degrees and then temporarily decreased, thereafter increasing again. These results strongly suggest that GtH1 and GtH2 are expressed by different cells. The fact that GtH1 and GtH2 cells appear just before histological gonadal differentiation at all temperatures, together with the peak of GtH1 and GtH2 cell number during the temperature-sensitive period, suggests that GtHs are related to sex differentiation or TSD in O. bonariensis.
authors.affiliation: Instituto de Investigaciones Biotecnológicas/Instituto Tecnológico de Chascomús, (CONICET-UNSAM), Camino de Circunvalación Laguna, Km 6. cc 164 (7130), Chascomús, Provincia de Buenos Aires, Argentina. lmiranda@intech.gov.ar
authors.forename: L A
authors.initials: LA
authors.lastname: Miranda
authors.forename: C A
authors.initials: CA
authors.lastname: Strüssmann
authors.forename: G M
authors.initials: GM
authors.lastname: Somoza
mesh_terms: D000818:Animals; D002452:Cell Count; D003001:Cloning, Molecular; D005260:Female; D005399:Fishes / Q000502:physiology*; D006065:Gonadotropins, Pituitary / Q000378:metabolism*; D007150:Immunohistochemistry; D007814:Larva; D008297:Male; D010902:Pituitary Gland / Q000166:cytology* / Q000378:metabolism*; D012733:Sex Differentiation / Q000502:physiology*
chemical_list: D006065:Gonadotropins, Pituitary; C097582:gonadotropin I beta-subunit, bass; C097583:gonadotropin II beta subunit, bass