Detection of Toxoplasma gondii in the milk of experimentally infected lactating cats.
Tachyzoites of Toxoplasma gondii have been found in the milk of sheep, goats, cows and mice and infection by ingestion of raw goat milk has been documented in humans. Lactational transmission from infected cats to their kittens is suspected but the organism has not been detected in the milk. The purpose of this study was to demonstrate the presence of T. gondii in the milk of experimentally infected cats. Pregnant specific pathogen free cats were inoculated orally with T. gondii at various times prior to parturition. Feces were examined for oocyst shedding after sugar solution centrifugation. Milk was collected for polymerase chain reaction (PCR) and bioassay in mice. T. gondii was detected in the milk of five of six cats by either bioassay or PCR.
Powell
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CC
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, CO 80523, USA. cpowell@uth.colostate.edu
Brewer
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Lappin
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MR
D000818:Animals; D000833:Animals, Suckling; D001681:Biological Assay / Q000379:methods / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000635:transmission; D002415:Cats; D005243:Feces / Q000469:parasitology; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D007774:Lactation; D008892:Milk / Q000469:parasitology*; D009865:Oocytes; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000635:transmission