title: Interaction of lyssaviruses with the low-affinity nerve-growth factor receptor p75NTR.
abstract: The low-affinity nerve-growth factor receptor p75NTR interacts in vitro with the rabies virus (RV) glycoprotein and serves as a receptor for RV. The Lyssavirus genus comprises seven genotypes (GTs) of rabies and rabies-related viruses. The ability of p75NTR to interact with the glycoprotein of representative lyssaviruses from each GT was investigated. This investigation was based on a specific binding assay between BSR cells infected with a lyssavirus and Spodoptera frugiperda (Sf21) cells expressing p75NTR on the cell surface. A specific interaction was observed with the glycoprotein of GT 1 RV (challenge virus standard or Pasteur virus strains) as well as wild-type RV and the glycoprotein of GT 6 European bat lyssavirus type 2. In contrast, no interaction was detected with the glycoprotein of lyssaviruses of GTs 2-5 and 7. Therefore, p75NTR is only a receptor for some lyssavirus glycoproteins, indicating that the other GTs must use an alternative specific receptor.
authors.affiliation: Laboratoire de Génétique des Virus, Bat 14B, Centre National de la Recherche Scientifique, 91198, Gif sur Yvette Cedex, France1.
authors.forename: Christine
authors.initials: C
authors.lastname: Tuffereau
authors.affiliation: Laboratoire des Lyssavirus, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France2.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Desmézières
authors.affiliation: Laboratoire de Génétique des Virus, Bat 14B, Centre National de la Recherche Scientifique, 91198, Gif sur Yvette Cedex, France1.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Bénéjean
authors.affiliation: Laboratoire des Lyssavirus, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France2.
authors.forename: Corinne
authors.initials: C
authors.lastname: Jallet
authors.affiliation: Laboratoire de Génétique des Virus, Bat 14B, Centre National de la Recherche Scientifique, 91198, Gif sur Yvette Cedex, France1.
authors.forename: Anne
authors.initials: A
authors.lastname: Flamand
authors.affiliation: Laboratoire des Lyssavirus, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France2.
authors.forename: Noël
authors.initials: N
authors.lastname: Tordo
authors.affiliation: Laboratoire des Lyssavirus, Institut Pasteur, 28, rue du Dr. Roux, 75724 Paris Cedex 15, France2.
authors.forename: Pierre
authors.initials: P
authors.lastname: Perrin
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D005838:Genotype; D006023:Glycoproteins / Q000378:metabolism; D007836:Lassa virus / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013045:Species Specificity; D018411:Spodoptera; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014764:Viral Proteins