Expression and function of CD43 and CDw60 on T cells from patients with atopic dermatitis.
Signalling via the CD43 and CDw60 epitopes has been reported as providing two novel pathways of T-lymphocyte activation. In Wiskott-Aldrich syndrome, which has atopic eczema-like skin symptoms, there is a defective expression of CD43, while CDw60 is strongly expressed on T cells from rheumatoid arthritis synovial fluid and from psoriatic skin lesions, and on blood mononuclear cells from patients with cutaneous T-cell lymphoma. We therefore studied the expression and function of these phenotypes on peripheral blood mononuclear cells and on CD4+ and CD8+ T-cell subsets from patients with atopic dermatitis. We observed a significant increase in the percentage of CD43+ cells among the blood mononuclear cells in patients with atopic dermatitis and an enhanced proliferation of CD4+ T cells following stimulation with anti-CD43 antibody. There were no changes in the CDw60 expression or function after stimulation with anti-CDw60 antibody. Thus, CD43 expression was not decreased but rather increased in blood mononuclear cells from patients with atopic dermatitis, whereas CDw60 expression did not differ from healthy controls.
Higashi
N
N
Department of Dermatology, Marselisborg Hospital, University of Aarhus, Denmark. nhi_ton@hotmail.com
Wu
K
K
Grønhøj Larsen
C
C
Deleuran
M
M
Kawana
S
S
Yamamoto
K
K
Thestrup-Pedersen
K
K
D000328:Adult; D015703:Antigens, CD / Q000097:blood* / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D006801:Humans; D051917:Leukosialin; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D012795:Sialoglycoproteins / Q000097:blood* / Q000276:immunology; D013601:T-Lymphocytes
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; C064741:CDw60 antigen; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins