Differential Cellular Distribution of Retinoic Acid during Staurosporine Potentiation of Retinoic Acid-Induced Granulocytic Differentiation in Human Leukemia HL-60 Cells.
Pretreatment of cells with staurosporine, a protein kinase C (PKC) inhibitor, was found to potentiate the granulocytic differentiation induced by a brief (2 h) retinoic acid treatment. By cell cycle analysis, staurosporine was found to have little effect on the cell cycle. Retinoic acid was distributed equally in the nuclei (40%) and in the plasma membrane (40%) of staurosporine-pretreated cells while less than 20% of retinoic acid was found in the membrane of control non-staurosporine-pretreated cells during the retinoic acid-induced differentiation. These results indicate that the enhancing effect of staurosporine may be somehow associated with the localization of retinoic acid in the plasma membrane of the cell. Copyright 1995 S. Karger AG, Basel
Yung
B.Y.-M.
BY
Cancer Biochemistry Laboratory, Department of Pharmacology, Chang Gung Medical College, Tao-yuan, Taiwan, Republic of China.
Hui
E.K.-W.
EK