[The medium conditioned incubation with rat heart cells maintains the properties of ES cells].
17 kinds of conditioned media were selected by plating tests, propagation and other tests with two cell lines, C-19-2 and MESPU-13. The results suggested that the rat heart cells conditioned media (RH-CM) can inhibit the spontaneous differentiation effectively, maintain the normal diploid karyotypes and promote adherence and proliferation of mouse ES cells. The ES cells were propagated in RH-CM to the 20th passage remaining their pluripotent in vivo and in vitro differentiation ability. RH-CM can be used as supplement of ES cell media. ES cells which are cultured in media containing 70% RH-CM and on PMEF feeder can maintain their undifferentiation state and diploid karyotype. RT-PCR detection suggested that there was mLIF expression in rat heart cells.
Meng
G L
GL
College of Life Sciences, Peking University, Beijing 100871, China. menggl@263.net
Teng
L
L
Zou
J Z
JZ
Xue
Y F
YF
Shang
K G
KG
D000818:Animals; D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D004171:Diploidy; D004622:Embryo, Mammalian / Q000166:cytology*; D006131:Growth Inhibitors / Q000235:genetics; D006321:Heart / Q000502:physiology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000235:genetics; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013234:Stem Cells / Q000502:physiology*
D017077:Culture Media, Conditioned; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger