title: Variation of microbial communities in soil, rhizosphere, and rhizoplane in response to crop species, soil type, and crop development.
abstract: We investigated the influence of plant species, soil type, and plant development time on the shaping of microbial communities in soil and in association with roots. The sample group consisted of a total of 32 microcosms in three habitats: soil, rhizosphere, and rhizoplane. Communities were represented by the patterns of a sequence-specific separation of rRNA target sequences. Effects of experimental parameters were classified by a cluster analysis of pattern similarities. The type of plant species (clover, bean, or alfalfa) had the greatest effect in plant-associated habitats and also affected soil patterns. Plant development had a minor habitat-dependent effect that was partly obscured by replicate variation. The results stress the applicability of biased community representations in an analysis of induced variation.
authors.affiliation: Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Braunschweig, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Wieland
authors.forename: R
authors.initials: R
authors.lastname: Neumann
authors.forename: H
authors.initials: H
authors.lastname: Backhaus
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000254:growth & development; D018556:Crops, Agricultural / Q000254:growth & development; D017753:Ecosystem; D004587:Electrophoresis, Agar Gel / Q000379:methods; D018517:Plant Roots / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil