title: Senescence and cytoskeleton: overproduction of vimentin induces senescent-like morphology in human fibroblasts.
abstract: One characteristic feature of senescent fibroblasts is flat, enlarged, and heterogeneous cell shapes. The present study was aimed to understand the structural basis of the senescent cell morphology. SDS-gel electrophoresis as well as western blotting demonstrated that there occurred a prominent protein band about 57 kDa in the senescent cells as compared with normal young or immortalized cells growing rapidly, and the protein was identified with a cytoskeletal protein, vimentin. In fact, senescent fibroblasts contained approximately threefold more vimentin protein, and fourfold more vimentin mRNA than young embryonic fibroblasts. In the senescent cells, vimentin cytoskeleton occurred as densely bundled filaments in parallel with the long axis of cell bodies, whereas in young or actively growing cells it showed short and thin vimentin filaments or fur-like irregular networks. It was further demonstrated that senescent cell shapes could be induced when a vimentin expression construct was transfected in young fibroblasts. These results suggest that senescent fibroblasts overproduce vimentin protein, and the overproduced vimentin filaments bring about the senescent cell morphology.
authors.affiliation: Department of Anatomy and Cell Biology, Nagoya University School of Medicine, 65 Tsurumai-Cho, Showa-Ku, Nagoya 466-8550, Japan. knishio@med.nagoya-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Nishio
authors.forename: A
authors.initials: A
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Qiao
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: A
authors.initials: A
authors.lastname: Mimura
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016922:Cellular Senescence; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000235:genetics / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014746:Vimentin; D049452:Green Fluorescent Proteins