[Effect of T lymphocyte in the acute rejection of liver xenograft].
OBJECTIVE
To study whether T cell take part in the acute rejection of liver corcondant xenograft.


METHODS
The orthotopic liver transplantation model introduced by Hariharas was established. In grafts, CD4, CD8 and Fas-L were examined by immunohistochemistry at day 1, 3, 5, 7 post-transplantant respectively, and apoptosis in grafts was observed by histology and TUNEL (Terminal Deoxynucleotidyl Transferse-mediated dUTP nick-end Labelling,).


RESULTS
In xenogenic grafts, T cell infilitration occurred at day 3, peaked at day 5, including CD4, CD8 subsets. The expression of Fas-L was found at day 4, peaked at day 5-6. The more severe the acute rejection is, the more the quantities of the expression of Fas-L is, the more the quantities of apoptosis is in grafts.


CONCLUSIONS
T lymphocytes participate in the acute rejection of liver xenograft.
Tan
J
J
Department of Hepatobiliary, Fuzhou General Hospital, PLA, Fuzhou 350025 China.
Wang
L
L
Yang
J
J
D000818:Animals; D017209:Apoptosis; D006224:Cricetinae; D053222:Fas Ligand Protein; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D016031:Liver Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014183:Transplantation, Heterologous
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins