Identification and sequencing the juvenile spermatogonial depletion critical interval on mouse chromosome 1 reveals the presence of eight candidate genes.
In mice, the recessive, non-pleiotropic, juvenile spermatogonial depletion (jsd) mutation results in a single wave of spermatogenesis, followed by failure of type A spermatogonial stem cells to differentiate, rendering adult males sterile. As part of an effort to identify the gene underlying this mutation, we report here the construction of a high-resolution genetic map involving more than 1000 meioses and 24 polymorphic loci. Our data define a critical jsd interval of approximately 0.4 cM at 49 cM on mouse chromosome 1, between D1Mit215 and 257SP6. We have constructed a physical map spanning the region comprising 24 overlapping BACs. Eighteen of these BACs have been fully sequenced, or are in draft form, allowing us to annotate approximately 2.5 Mb of DNA surrounding the jsd locus. The critical 0.4 cM jsd interval corresponds to a physical distance of approximately 1.5 Mb. Eight genes have been identified in this interval, two of which appear to be possible candidates for the jsd mutation.
Boettger-Tong
H L
HL
Department of Obstetrics and Gynecology, Baylor College of Medicine, 6550 Fannin, Suite 861, Houston, TX 77030, USA.
Rohozinski
J
J
Agoulnik
A I
AI
Dohmae
K
K
Nishimune
Y
Y
Levy
N
N
Bishop
C E
CE
D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes; D044842:Cullin Proteins; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009154:Mutation; D017422:Sequence Analysis, DNA; D013091:Spermatogenesis; D013093:Spermatogonia / Q000502:physiology*; D013737:Testis / Q000033:anatomy & histology; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; C503646:Cul3 protein, mouse; D044842:Cullin Proteins