title: Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration.
abstract: Using N2 cavitation, we established a protocol to prepare the active mitochondria from Plasmodium falciparum showing a higher succinate dehydrogenase activity than previously reported and a dihydroorotate-dependent respiration. The fact that fumarate partially inhibited the dihydroorotate dependent respiration suggests that complex II (succinate-ubiquinone reductase/quinol-fumarate reductase) in the erythrocytic stage cells of P. falciparum functions as a quinol-fumarate reductase.
authors.affiliation: Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113-0033, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Takashima
authors.forename: S
authors.initials: S
authors.lastname: Takamiya
authors.forename: S
authors.initials: S
authors.lastname: Takeo
authors.forename: F
authors.initials: F
authors.lastname: Mi-ichi
authors.forename: H
authors.initials: H
authors.lastname: Amino
authors.forename: K
authors.initials: K
authors.lastname: Kita
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005650:Fumarates / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D010963:Plasmodium falciparum / Q000201:enzymology / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000032:analysis / Q000378:metabolism*
chemical_list: D005650:Fumarates; C004768:4,5-dihydroorotic acid; D009963:Orotic Acid; C032005:fumaric acid; D013385:Succinate Dehydrogenase; D010100:Oxygen