title: Intrachromosomal genomic instability in human sporadic colorectal cancer measured by genome-wide allelotyping and inter-(simple sequence repeat) PCR.
abstract: We have used genome-wide allelotyping with 348 polymorphic autosomal markers spaced, on average, 10 cM apart to quantitate the extent of intrachromosomal instability in 59 human sporadic colorectal carcinomas. We have compared instability measured by this method with that measured by inter-(simple sequence repeat) PCR and microsatellite instability assays. Instability quantitated by fractional allelic loss rates was found to be independent of that detected by microsatellite instability analyses but was weakly associated with that measured by inter-(simple sequence repeat) PCR. A set of seven loci were identified that were most strongly associated with elevated rates of fractional allelic loss and/or inter-(simple sequence repeat) PCR instability; these seven loci were on chromosomes 3, 8, 11, 13, 14, 18, and 20. A lesser association was seen with two loci flanking p53 on chromosome 17. Coordinate loss patterns for these loci suggest that at least two separate sets of cooperating loci exist for intrachromosomal genomic instability in human colorectal cancer.
authors.affiliation: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA. garth.anderson@roswellpark.org
authors.forename: G R
authors.initials: GR
authors.lastname: Anderson
authors.forename: B M
authors.initials: BM
authors.lastname: Brenner
authors.forename: H
authors.initials: H
authors.lastname: Swede
authors.forename: N
authors.initials: N
authors.lastname: Chen
authors.forename: W M
authors.initials: WM
authors.lastname: Henry
authors.forename: J M
authors.initials: JM
authors.lastname: Conroy
authors.forename: M J
authors.initials: MJ
authors.lastname: Karpenko
authors.forename: J P
authors.initials: JP
authors.lastname: Issa
authors.forename: J D
authors.initials: JD
authors.lastname: Bartos
authors.forename: J K
authors.initials: JK
authors.lastname: Brunelle
authors.forename: G P
authors.initials: GP
authors.lastname: Jahreis
authors.forename: M S
authors.initials: MS
authors.lastname: Kahlenberg
authors.forename: M
authors.initials: M
authors.lastname: Basik
authors.forename: S
authors.initials: S
authors.lastname: Sait
authors.forename: M A
authors.initials: MA
authors.lastname: Rodriguez-Bigas
authors.forename: N J
authors.initials: NJ
authors.lastname: Nowak
authors.forename: N J
authors.initials: NJ
authors.lastname: Petrelli
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: D L
authors.initials: DL
authors.lastname: Stoler
mesh_terms: D000483:Alleles; D002869:Chromosome Aberrations; D015179:Colorectal Neoplasms / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods