[Biological activity detection of mouse type I soluble interleukin-1 receptor].
OBJECTIVE
To comfirm that the recombinant sIL-1R had biological activity.


METHODS
With biotin labed human IL-1 beta as ligand, protein blotting receptor-ligand binding assay and enzyme-linked immunological receptor-ligand binding assay were developed to detect the expression of sIL-1R I gene in insect cells (Sf9). Meanwhile, the blockage assay of IL-1 beta biological activity was used to detect the function of recombinant sIL-1R I.


RESULTS
It showed that the recombinant sIL-1R I possessed immunological activity and ligand binding activity and could be secreted into the medium.


CONCLUSIONS
The three assays could be used for detecting biological activity of recombinant sIL-1R I.
Zhang
Z
Z
Institute of Medicinal Biotechnology, CAMS and PUMC, Beijing 100050.
Li
Y
Y
Wu
Q
Q
D000818:Animals; D001710:Biotin; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D007313:Insecta / Q000166:cytology / Q000378:metabolism; D007375:Interleukin-1; D008024:Ligands; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology*; D053573:Receptors, Interleukin-1 Type I; D011994:Recombinant Proteins
D007375:Interleukin-1; D008024:Ligands; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D011994:Recombinant Proteins; D001710:Biotin