Establishment of CD7+ human myeloma sister cell lines, KMS-21-PE and KMS-21-BM, carrying t(11;14) and t(8;14).
Two new human myeloma cell lines were established from pleural effusion and bone marrow malignant cells derived from a single patient, who manifested hyperammonemia associated with multiple myeloma, and these were characterized. Both lines possess t(11;14)(q13;q32) and t(8;14)(q24;q32) reciprocal translocations and overexpress cyclin D1, but not c-myc. Human myeloma lines including these new lines produced and secreted excess ammonia into culture medium more than non-myelomatous hematological cell lines. In addition, these two lines were revealed to have high surface CD7 expression correlated with relatively high mRNA expression by MP-RT-PCR. Among 8 human myeloma lines, half of them revealed significant surface expression of CD7 and a positive correlation between expression levels of protein and message. CD7 message was also detected in surface negative lines. Consequently, there may be posttranslational regulation of the CD7 molecule, whose cellular biological role in expressing cells has not been elucidated.
Otsuki
T
T
Department of Hygiene, Kawasaki Medical School, Kurashiki, Okayama, Japan. takemi@med.kawasaki-m.ac.jp
Wada
H
H
Nakazawa
N
N
Taniwaki
M
M
Kouguchi
K
K
Ohkura
M
M
Uehira
K
K
Isoda
K
K
Yata
K
K
Sakaguchi
H
H
Yawata
Y
Y
Ueki
A
A
Yamada
O
O
D000328:Adult; D000641:Ammonia / Q000378:metabolism; D019011:Antigens, CD7 / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000473:pathology; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D002898:Chromosomes, Human, Pair 8; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D022124:Hyperammonemia / Q000209:etiology / Q000473:pathology; D008297:Male; D009101:Multiple Myeloma / Q000150:complications / Q000235:genetics / Q000473:pathology*; D016066:Pleural Effusion, Malignant / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
D019011:Antigens, CD7; D016271:Proto-Oncogene Proteins c-myc; D019938:Cyclin D1; D000641:Ammonia