title: Evolution of TEM-related extended-spectrum beta-lactamases in Korea.
abstract: TEM-52, differing from TEM-1 by having the substitutions Glu-104-->Lys, Met-182-->Thr, and Gly-238-->Ser, has previously been described as the most prevalent extended-spectrum beta-lactamase (ESBL) in Korea. In a further survey, we discovered the ESBLs TEM-15, which is like TEM-52 but lacks the substitution at residue 182, and TEM-88, which is like TEM-52 with an additional Gly-196-->Asp substitution. TEM-88 retained the activity of TEM-52 against moxalactam. Otherwise, the kinetic properties of the three ESBLs failed to show an advantage to this evolution.
authors.affiliation: Department of Internal Medicine, College of Medicine, Dankook University, Chonan, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Pai
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: E H
authors.initials: EH
authors.lastname: Choi
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: G A
authors.initials: GA
authors.lastname: Jacoby
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005075:Biological Evolution; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D007723:Korea; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C445691:TEM-52 beta-lactamase; C445690:TEM-88 beta-lactamase; D001618:beta-Lactamases