title: Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2.
abstract: Gingival epithelial cells are a central component of the barrier between oral microflora and internal tissues. Host responses to periodontopathic bacteria and surface components containing fimbriae are thought to be important in the development and progression of periodontal diseases. To elucidate this mechanism, we established immortalized human gingival epithelial cells (HGEC) that were transfected with human papillomavirus. HGEC predominantly expressed Toll-like receptor (TLR) 2, but not TLR4 or CD14. They also induced interleukin-8 (IL-8) production when stimulated with Porphyromonas gingivalis fimbriae and Staphylococcus aureus peptidoglycan, but not Escherichia coli-type synthetic lipid A. Furthermore, an active synthetic peptide composed of residues 69 to 73 (ALTTE) of the fimbrial subunit protein, derived from P. gingivalis and similar to a common component of cell wall peptidoglycans in parasitic bacteria, N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), significantly induced IL-8 production and NF-kappaB activation in HGEC, and these cytokine-producing activities were augmented by a complex of soluble CD14 and lipopolysaccharide-binding protein (LBP). IL-8 production in HGEC stimulated with these bacterial components was clearly inhibited by mouse monoclonal antibody to human TLR2. These findings suggest that P. gingivalis fimbrial protein and its active peptide are capable of activating HGEC through TLR2.
authors.affiliation: Department of Oral Microbiology, Asahi University School of Dentistry, Motosu-gun, Gifu 501-0296, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Asai
authors.forename: Y
authors.initials: Y
authors.lastname: Ohyama
authors.forename: K
authors.initials: K
authors.lastname: Gen
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
mesh_terms: D001426:Bacterial Proteins / Q000276:immunology*; D029721:Drosophila Proteins; D004847:Epithelial Cells / Q000276:immunology*; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005881:Gingiva / Q000276:immunology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D007633:Keratins / Q000302:isolation & purification; D018950:Lipopolysaccharide Receptors / Q000302:isolation & purification; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D009842:Oligopeptides / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
chemical_list: D001426:Bacterial Proteins; D029721:Drosophila Proteins; D016209:Interleukin-8; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D009842:Oligopeptides; D010446:Peptide Fragments; D011498:Protein Precursors; D011956:Receptors, Cell Surface; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; C032253:involucrin; D007633:Keratins