Human esophageal cancer cell death mediated by apoptosis-inducing nucleosides from CD57+HLA-DRbright natural suppressor cell line.
We approached the induction of apoptosis in human esophageal cancer cells (T.Tn) by apoptosis-inducing nucleosides (AINs) from CD57+HLA-DRbright-natural suppressor (57.DR-NS) cell line originated in decidua. The 57.DR-NS cells generated apoptosis in T.Tn cells by AINs released into the cultures. We isolated a series of AINs by high performance liquid chromatography (HPLC) from 57.DR-NS cell culture fluids. Each AIN and its mixture induced apoptotic cell death in T.Tn cells, detected by DNA fragmentation in agarose gels, terminal deoxynucleotide transferase mediated dUTP-nick end labeling (TUNEL) method and accumulation of sub-G1 DNA content with flow cytometry. Furthermore, we found the occurrence of DNA strand breaks followed by the activation of caspase-3 during AINs-induced apoptosis in T.Tn cells. Thus, we validated that AINs could induce apoptosis in T.Tn cells mediated through DNA strand breaks and activation of caspase-3.
Mori
T
T
Department of Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-0071, Japan. ts-mori@hgc.ims.u-tokyo.ac.jp
Guo
M
M
Jin
A
A
Li
X
X
Mori
E
E
D017209:Apoptosis; D018959:CD57 Antigens / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D020169:Caspases / Q000502:physiology; D002453:Cell Cycle / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D004273:DNA, Neoplasm / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004938:Esophageal Neoplasms / Q000473:pathology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D020287:In Situ Nick-End Labeling; D009705:Nucleosides / Q000378:metabolism*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D014407:Tumor Cells, Cultured
D018959:CD57 Antigens; D004273:DNA, Neoplasm; D006684:HLA-DR Antigens; D009705:Nucleosides; D020169:Caspases