NaCl consumption is attenuated in female KCNE1 null mutant mice.
The role of potassium channels in the regulation of NaCl intake has not been investigated previously. One potassium channel, KCNQ1, and its regulator, KCNE1, are expressed in salivary glands and kidneys, and KCNE1 null mutant mice are deficient in KCNQ1 potassium currents. To understand the role of the KCNQ1/KCNE1 channel complex in NaCl taste and intake, we compared the NaCl consumption of KCNE1 +/+ (129/Sv), KCNE1 +/-, and KCNE1 -/- mice using two-bottle intake tests and lick rate tests. Although KCNE1 +/+ and KCNE1 +/- mice exhibited consumption patterns for 75-150 mM NaCl solutions considered typical for 129/Sv mice, the KCNE1 -/- null mutant 129/Sv mice were indifferent to or rejected them. This effect was observed in female mice only, required prior exposure to NaCl solutions, and the extent of rejection was greater after prior exposure to 150 mM NaCl solution than 75 mM NaCl solution. No differences were observed in the avidity for KCl solutions or in lick rates of naive mice for 150 or 300 mM NaCl solutions. These results demonstrate that a single potassium channel gene can influence voluntary NaCl intake. We speculate that disruption of the KCNE1 gene impairs sodium metabolism in female mice drinking high levels of 150 mM NaCl, which causes malaise that becomes associated with NaCl taste, and as a consequence, reduced preference for NaCl.
Puchalski
R B
RB
Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104, USA.
Kelly
E
E
Bachmanov
A A
AA
Brazier
S P
SP
Kuang
J
J
Arrighi
I
I
Barhanin
J
J
Tordoff
M G
MG
D000818:Animals; D004305:Dose-Response Relationship, Drug; D004326:Drinking / Q000502:physiology*; D005260:Female; D007668:Kidney / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D012469:Salivary Glands / Q000378:metabolism; D012965:Sodium Chloride / Q000008:administration & dosage*; D013649:Taste / Q000235:genetics* / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000235:genetics*
D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; C059983:potassium channel protein I(sk); D012965:Sodium Chloride