In vitro enzyme evolution: the screening challenge of isolating the one in a million.
The generation of large mutant libraries for in vitro enzyme evolution presents the challenge of effectively screening libraries of 10(4)-10(7) mutants on the basis of simultaneously assaying their biocatalytic activity. In this review, we highlight the main steps involved in this process, describe the alternative approaches to address this challenge, survey the state-of-the-art technology and assess achievements already made. It is anticipated that, as a result of the expected accomplishment of further improvements in high-throughput screening that will allow routine screening of whole libraries, the number of useful new and improved enzymes derived through in vitro enzyme evolution will expand rapidly in the near future.
Cohen
N
N
Dept of Molecular Microbiology and Biotechnology, Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel.
Abramov
S
S
Dror
Y
Y
Freeman
A
A
D018914:Cells, Immobilized / Q000201:enzymology; D019020:Directed Molecular Evolution / Q000639:trends*; D004795:Enzyme Stability / Q000235:genetics; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D015723:Gene Library; D016296:Mutagenesis / Q000235:genetics*; D012196:Review Literature as Topic; D012641:Selection, Genetic
D004798:Enzymes