title: Providing a Microenvironment for the Development of Human CD34+ Hematopoietic Cells in SCID Mice.
abstract: In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed. Copyright 1997 S. Karger AG, Basel
authors.affiliation: Henry M. Jackson Foundation for the Advancement of Military Medicine and Walter Reed Army Institute of Research, Rockwille, Md., USA.
authors.forename: S.
authors.initials: S
authors.lastname: Kaushal
authors.forename: V.F.
authors.initials: VF
authors.lastname: La Russa
authors.forename: E.R.
authors.initials: ER
authors.lastname: Hall
authors.forename: S.
authors.initials: S
authors.lastname: Gartner
authors.forename: J.H.
authors.initials: JH
authors.lastname: Kim
authors.forename: L.P.
authors.initials: LP
authors.lastname: Perera
authors.forename: Z.
authors.initials: Z
authors.lastname: Yu
authors.forename: S.W.
authors.initials: SW
authors.lastname: Kessler
authors.forename: J.D.
authors.initials: JD
authors.lastname: Mosca