Chromium (VI)-induced production of reactive oxygen species, change of plasma membrane potential and dissipation of mitochondria membrane potential in Chinese hamster lung cell cultures.
OBJECTIVE
To examine whether Reactive Oxygen Species (ROS) is generated, and whether plasma membrane potential and mitochondrial membrane potential are depolarized in Chinese Hamster Lung (CHL) cell lines exposed to Cr (VI).


METHODS
CHL cells were incubated with Cr(VI) at 10 mumol/L, 2.5 mumol/L, 0.65 mumol/L for 3 and 6 hours, respectively. The production of ROS was performed by using 2,7-dichlorofluorescin diacetate; The changes in plasma membrane potential were estimated using fluorescent cationic dye DiBAC4; And the changes in mitochondria membrane potential were estimated using fluorescent dye Rhodamine 123.


RESULTS
The ROS levels in CHL cells increased in all treated groups compared with the control group (P < 0.01); The plasma membrane potential and mitochondrial membrane potential in CHL cells dissipated after incubated with Cr(VI) at 10 mumol/L for 3 hours and 6 hours (P < 0.01), at 2.5 mumol/L for 6 hours (P < 0.01 or 0.05).


CONCLUSIONS
Cr(VI) causes the dissipation of plasma membrane potential and mitochondrial membrane potential in CHL cell cultures, and Cr(VI)-induced ROS may play a role in the injuries.
Xie
Y
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Department of Toxicology, Chinese Academy of Preventive Medicine, Beijing 100050, China. maze12@163.net
Zhuang
Z X
ZX
D000818:Animals; D002274:Carcinogens, Environmental / Q000009:adverse effects*; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000502:physiology*; D002857:Chromium / Q000009:adverse effects*; D006224:Cricetinae; D003412:Cricetulus; D008168:Lung / Q000166:cytology; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology*; D017382:Reactive Oxygen Species
D002274:Carcinogens, Environmental; D017382:Reactive Oxygen Species; D002857:Chromium; C074702:chromium hexavalent ion