title: Mediated electrochemical detection of catechol by tyrosinase-based poly(dicarbazole) electrodes.
abstract: A new dicarbazole derivative functionalised by an N-hydroxysuccinimide group has been synthesised and electrochemically characterised. Upon oxidative electropolymerisation of this monomer in organic electrolytes, electroactive poly(dicarbazole) films were formed on platinum electrodes. The subsequent chemical grafting of tyrosinase on the poly(dicarbazole) film was easily performed by immersion in an enzymatic aqueous solution. The amperometric response of the resulting biosensors to catechol has been studied at -0.2 V vs. saturated calomel electrode (SCE). Since the reduction of quinone generates radicals which may induce electrode fouling, thionine, a phenothiazine dye, was covalently bound to the poly(dicarbazole) backbone as it mediates the reduction of quinoid products and therefore induces an enhancement of the performance of the tyrosinase-based biosensor.
authors.affiliation: Laboratoire d'Electrochimie Organique et de Photochimie Redox, UMR CNRS 5630, Université Joseph Fourier Grenoble 1, 301 rue de la Chimie, BP 53, 38041 Cedex 9, Grenoble, France. serge.cosnier@ujf-grenoble.fr
authors.forename: S
authors.initials: S
authors.lastname: Cosnier
authors.forename: S
authors.initials: S
authors.lastname: Szunerits
authors.forename: R S
authors.initials: RS
authors.lastname: Marks
authors.forename: J P
authors.initials: JP
authors.lastname: Lellouche
authors.forename: K
authors.initials: K
authors.lastname: Perie
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015374:Biosensing Techniques; D002138:Calibration; D002227:Carbazoles / Q000737:chemistry*; D004156:Catechol Oxidase / Q000378:metabolism; D002396:Catechols / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D018942:Macrolides; D008956:Models, Chemical; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010640:Phenothiazines / Q000737:chemistry / Q000494:pharmacology; D013053:Spectrophotometry
chemical_list: D000900:Anti-Bacterial Agents; D002227:Carbazoles; D002396:Catechols; D018942:Macrolides; D010640:Phenothiazines; D004156:Catechol Oxidase; D014442:Monophenol Monooxygenase; C031637:phenothiazine; C034221:catechol; D010100:Oxygen; C009469:thionine