title: [Study on cis-acting elements of rat glutathione S-tranferase P1 gene].
abstract: OBJECTIVE
  To investigate the cis-acting regulatory DNA elements in 3.0 kb 5' flanking upstream region of rat glutathione S-tranferase (rGSTP1) gene.
  
  
  METHODS
  Using exonuclease III (Exo III) and S1 nuclease, a series of deletions were made in the 3.0 kb region, then fused into highly sensitive luciferase reporter vector. The deleted mutants were transfected into HeLa cells. Luciferase activities of the transfected cell lysates were assayed.
  
  
  RESULTS
  Deleted from -2.9 kb to -2.3 kb, luciferase activity had dropped about 67% (P < 0.05). The element between -2.5 kb and -2.2 kb could increase gene expression activity regardless of orientation and position. Further deleted from -1.8 kb to -1.3 kb, luciferase activity increased about three times (P < 0.05).
  
  
  CONCLUSIONS
  There are enhancer element between -2.5 kb and -2.2 kb and a negative regulatory sequence between -1.8 kb and -1.3 kb in the upstream region of rGSTP1 gene.
authors.affiliation: Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Quan
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Fang
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection
chemical_list: D007527:Isoenzymes; D008156:Luciferases; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C496558:Gstp1 protein, rat