title: Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors.
abstract: Cofactor requirement was determined for the heterologous expressed phytoene desaturases from the cyanobacterium Synechococcus and the higher plant Gentiana lutea. The cyanobacterial enzyme is dependent on either NAD(P) or plastoquinone, whereas only quinones such as plastoquinone can function as a cofactor for the phytoene desaturase from G. lutea. Enzyme kinetic studies were carried out to determine a possible competition between the cofactors and the bleaching herbicide norflurazon. For the Synechococcus enzyme, competition between norflurazon and NADP, as well as plastoquinone, could be demonstrated. The K(m) values for these cofactors were 6.6 mM and 0.23 microM, respectively. Inhibition of the phytoene desaturase from G. lutea by norflurazon was also competitive with respect to plastoquinone. The K(m) values of both enzymes for plastoquinone were very close.
authors.affiliation: Botanisches Institut 213, J.W. Goethe Universität, P.O. Box 111932, D-60054 Frankfurt, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Breitenbach
authors.forename: C
authors.initials: C
authors.lastname: Zhu
authors.forename: G
authors.initials: G
authors.lastname: Sandmann
mesh_terms: D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D029965:Gentianaceae / Q000201:enzymology; D006540:Herbicides / Q000494:pharmacology*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011724:Pyridazines / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D004791:Enzyme Inhibitors; D006540:Herbicides; D011724:Pyridazines; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; C008677:norflurazone