[Inhibitory effect of phosphorothioae oligodeoxynucleotides on HBV replication and synthesis of antigen in vitro].
OBJECTIVE
To evaluate the effect of triplex forming oligodeoxynucleotides (TFO) and antisense oligodeoxynucleotides (ODNas) on the replication of HBV.


METHODS
A 21mer phosphorothioate TFO (TFO21) directed at 1734nt-1754nt sites in HBV core promoter and a 21 mer phosphorothioate ODNas (ODNas21) complementory to the initiation sites of pre C RNA and pregenomic RNA were synthesized. Effect of TFO21 and ODNas21 on HBV replication and synthesis of antigen were observed in 22.1.5 cells.


RESULTS
Both TFO21 and ODNas21 showed the inhibition to HBV replication and synthesis of antigen while, ODNcon (control of 21 mer phosphorothioate oligodeoxynucleotides) showed little inhibitory effects. At concentration of 10 mumol/L, TFO21 and ODNas21 inhibited the synthesis of HBsAg and HBeAg by 57.5% and 77%; 61% and 79.6%, respectively. The mixture of TFO21 and ODNas21 was more effective than TFO21 or ODNas21 alone. The inhibitions were dose-dependent. No toxicity was observed in the 22.1.5 cells treated with those oligodeoxynucleotides.


CONCLUSIONS
Triplex forming oligodeoxynucleotides and antisense oligodeoxynucleotides were both potent inhibitor, for HBV replication and synthesis of antigen.
Yang
L
L
Viral Hepatitis Research Unit, Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510630.
Yao
J
J
Deng
L
L
D000998:Antiviral Agents / Q000494:pharmacology*; D006511:Hepatitis B Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D018197:Hepatoblastoma / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000998:Antiviral Agents; D006511:Hepatitis B Antigens; D020319:Oligodeoxyribonucleotides, Antisense; D010710:Phosphates; C083120:phosphorodithioic acid