title: [Biological activity detection of mouse type I soluble interleukin-1 receptor].
abstract: OBJECTIVE
  To comfirm that the recombinant sIL-1R had biological activity.
  
  
  METHODS
  With biotin labed human IL-1 beta as ligand, protein blotting receptor-ligand binding assay and enzyme-linked immunological receptor-ligand binding assay were developed to detect the expression of sIL-1R I gene in insect cells (Sf9). Meanwhile, the blockage assay of IL-1 beta biological activity was used to detect the function of recombinant sIL-1R I.
  
  
  RESULTS
  It showed that the recombinant sIL-1R I possessed immunological activity and ligand binding activity and could be secreted into the medium.
  
  
  CONCLUSIONS
  The three assays could be used for detecting biological activity of recombinant sIL-1R I.
authors.affiliation: Institute of Medicinal Biotechnology, CAMS and PUMC, Beijing 100050.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D001710:Biotin; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D007313:Insecta / Q000166:cytology / Q000378:metabolism; D007375:Interleukin-1; D008024:Ligands; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology*; D053573:Receptors, Interleukin-1 Type I; D011994:Recombinant Proteins
chemical_list: D007375:Interleukin-1; D008024:Ligands; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D011994:Recombinant Proteins; D001710:Biotin