The effect of a reconstituted basement membrane (matrigel) on a human salivary gland myoepithelioma cell line.
We have already demonstrated that a reconstituted basement membrane (Matrigel) is a key modulator of morphogenetic changes and cytodifferentiation of pleomorphic adenoma cells in culture. Myoepithelioma is considered to be a neoplasm closely related to pleomorphic adenoma and should experience similar induction processes. Thus, the aim of this study was to investigate whether Matrigel would influence myoepithelioma cells. We used a cell line derived from a human salivary gland plasmacytoid myoepithelioma (M1 cells) grown in a three-dimensional preparation of Matrigel. Phenotype differences were assessed using conventional light microscopy technique (haematoxylin and eosin) and phase and differential interference contrast (Nomarski). Immunofluorescence was carried out to detect smooth-muscle actin, laminin and type-IV collagen. M1 cells exhibited all proteins studied, showing a myoepithelial differentiation. M1 cells grown inside Matrigel presented morphological changes and changes in smooth-muscle actin status. By growing M1 cells inside Matrigel, it was possible to reproduce the tumour architecture with no duct-like structures. Based on our findings, we suggest that myoepithelioma would be derived from a cell with a commitment to myoepithelial differentiation. We also suggest that the mechanical properties of the matrix environment will likely regulate smooth-muscle actin expression in myoepithelioma.
de Oliveira
P T
PT
Department of Oral Pathology, School of Dentistry, University of São Paulo, SP, Brazil.
Jaeger
M M
MM
Miyagi
S P
SP
Jaeger
R G
RG
D000199:Actins / Q000032:analysis / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism*; D003094:Collagen; D024141:Collagen Type IV / Q000032:analysis / Q000378:metabolism; D003470:Culture Media; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000378:metabolism*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007797:Laminin / Q000032:analysis / Q000378:metabolism; D009208:Myoepithelioma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D011509:Proteoglycans; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000199:Actins; D001672:Biocompatible Materials; D024141:Collagen Type IV; D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen