title: Oligothiophene isothiocyanates as a new class of fluorescent markers for biopolymers.
abstract: The regioselective synthesis of fluorescent oligothiophene isothiocyanates is described. The isothiocyanates were reacted with bovine serum albumin (BSA) following standard procedures and the optical properties of the oligothiophene-BSA conjugates were analyzed as a function of oligomer concentration, time, and irradiation power. The oligothiophene-BSA conjugates were chemically very stable and their photoluminescence characteristics persisted unaltered for several months. Photoluminescence data relative to the conjugate of an oligothiophene-S,S-dioxide isothiocyanate with monoclonal anti-CD8 antibody are reported. No fluorescence quenching was observed following the binding of the isothiocyanate to the antibody and the conjugate displayed high chemical stability and photostability.
authors.affiliation: Consiglio Nazionale Ricerche, I.Co.C.E.A, Via Gobetti 101, 40129 Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Barbarella
authors.forename: M
authors.initials: M
authors.lastname: Zambianchi
authors.forename: O
authors.initials: O
authors.lastname: Pudova
authors.forename: V
authors.initials: V
authors.lastname: Paladini
authors.forename: A
authors.initials: A
authors.lastname: Ventola
authors.forename: F
authors.initials: F
authors.lastname: Cipriani
authors.forename: G
authors.initials: G
authors.lastname: Gigli
authors.forename: R
authors.initials: R
authors.lastname: Cingolani
authors.forename: G
authors.initials: G
authors.lastname: Citro
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D001704:Biopolymers / Q000032:analysis; D016827:CD8 Antigens / Q000276:immunology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013876:Thiophenes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D001704:Biopolymers; D016827:CD8 Antigens; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D017879:Isothiocyanates; D013876:Thiophenes; D012710:Serum Albumin, Bovine