Androgen metabolism in oyster Crassostrea gigas: evidence for 17beta-HSD activities and characterization of an aromatase-like activity inhibited by pharmacological compounds and a marine pollutant.
The annual reproductive cycle of oyster Crassostrea gigas depends on environmental factors, but its endocrine regulations are still unknown. Sexual steroids play important roles at this level in vertebrates, and some estradiol effects have been described in invertebrates such as bivalve mollusks. To question these roles in invertebrates, we studied androgen metabolism in C. gigas. Incubations of tissue homogenates with 14C-steroids such as androstenedione (A), testosterone (T), estrone (E1) and estradiol (E2), followed by TLC and HPLC, provide evidence for 17beta-hydroxysteroid dehydrogenases (17beta-HSDs, conversions of A into T, T into A, E1 into E2 and E2 into E1) and aromatase-like (A into E1) activities. The latter activity was further characterized by tritiated water release assay; it was time- and temperature-dependent. Furthermore, this oyster aromatase-like activity was inhibited by 4-hydroxyandrostenedione (IC(50) 0.456 microM) and by other pharmacological compounds including specific cytochrome P450 inhibitors (MR20494, miconazole) and a marine pollutant (tributyltin).
Le Curieux-Belfond
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Laboratoire de Biologie et Biotechnologies Marines (EA 962, URM 14 IFREMER), IBBA, Université de Caen, Esplanade de la Paix, 14032 Cedex, Caen, France. lcboliv@aol.com
Moslemi
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Mathieu
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Séralini
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D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000728:Androgens / Q000378:metabolism*; D000818:Animals; D047072:Aromatase Inhibitors; D002855:Chromatography, Thin Layer; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008825:Miconazole / Q000494:pharmacology; D010125:Ostreidae / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
D000728:Androgens; D047072:Aromatase Inhibitors; D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D014874:Water Pollutants, Chemical; D008825:Miconazole; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase