[Neuroendocrine mechanisms of impact by experimental stress on plasma glucose level in STZ KM mice].
OBJECTIVE
To explore the neuroendocrine mechanisms of effects by experimental stress on plasma glucose level in streptozotocin induced mice(STZ mice).


METHODS
Forty STZ mice were divided randomly into Group A (STZ mice, stress) and Group B (STZ mice, no stress), and 20 normal KM mice in Group C (normal mice, stress) and Group D (normal mice, no stress). Group A and Group C were exposed to multiple stressors (restrain, rotation, crowding) for 6 weeks. Before and after the experiment, blood glucose(BG) levels were measured. By the end of experiment, blood and hypothalamus samples were collected to measure plasma insulin(I) concentrations, insulin sensitivity index(ISI), plasma norepinphine(NE), epinphin(EPI) and beta endorphin(beta-EP) concentrations.


RESULTS
After stress, the BG levels significantly increased and I concentrations decreased in Group A. Group A showed a greater decrease of ISI than that in Group B. There was significant difference in plasma NE and EPI concentrations among groups, and Group A showed marked increase. There was no significant difference in beta-EP concentrations among groups. There were low to moderate correlations among these variables after stress.


CONCLUSIONS
The STZ mice likely have altered adrenergic sensitivity to stress, which might play a leading role in the neuroendocrine mechanism of hyperglycemia of STZ mice after stress. The beta-EP might participate in this process of regulating glucose metabolism.
Yao
S
S
Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Cai
W
W
Dai
X
X
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D007328:Insulin / Q000097:blood; D008297:Male; D051379:Mice; D009490:Neurosecretory Systems / Q000378:metabolism; D011897:Random Allocation; D013311:Streptozocin; D013312:Stress, Physiological / Q000097:blood*; D001615:beta-Endorphin / Q000378:metabolism
D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin; D001615:beta-Endorphin