title: Enzyme-mediated free radical polymerization of styrene.
abstract: Horseradish peroxidase-mediated polymerization of styrene at ambient temperature is reported. Molecular weight and yield of polystyrene were influenced by solvent, concentration of hydrogen peroxide, and initiator (beta-diketones, coumarin). THF:H2O (v/v) and hydrogen peroxide (0.082 mol/L) provided maximum yield of polymer (21.2% weight conversion of styrene to polystyrene) with 2,4-pentanedione as initiator. 1,3-Cyclopentanedione and dibenzoylmethane as initiators resulted in higher yield of polymer (approximately 60%) and a higher molecular weight (Mn = 96,504, polydispersity = 2.16), respectively. This enzymatic strategy was also used for the synthesis of polymers from styrene derivatives, 4-methylstyrene and 2-vinylnaphthalene, the latter resulting in a > 90% yield of polymer. The presence of the initiators in the polymer chains is reported.
authors.affiliation: Department of Chemical & Biological Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA.
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: D L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D005609:Free Radicals; D006735:Horseradish Peroxidase / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011137:Polystyrenes / Q000138:chemical synthesis*; D012997:Solvents; D013343:Styrenes / Q000737:chemistry
chemical_list: D005609:Free Radicals; D011137:Polystyrenes; D012997:Solvents; D013343:Styrenes; D006735:Horseradish Peroxidase