title: Behaviour of Listeria monocytogenes in packaged fresh mushrooms (Agaricus bisporus).
abstract: OBJECTIVE
  The aim of this study was to evaluate the potential of Listeria monocytogenes to grow in mushrooms packaged in two different types of PVC films when stored at 4 degrees C and 10 degrees C.
  
  
  RESULTS
  Mushrooms were packed in two polymeric films (perforated and nonperforated PVC) and stored at 4 degrees C and 10 degrees C. The carbon dioxide and oxygen content inside the packages, aerobic mesophiles, psychrotrophs, Pseudomonas spp., Listeria monocytogenes, faecal coliforms, Escherichia coli, anaerobic spores and major sensory factors were determined. The mushrooms packaged in nonperforated film and stored at 4 degrees C had the most desirable quality parameters (texture, development stage and absence of moulds). Listeria monocytogenes was able to grow at 4 degrees C and 10 degrees C in inoculated mushrooms packaged in perforated and nonperforated films between 1 and 2 log units during the first 48 h. After 10 d of storage, the populations of L. monocytogenes were higher in mushrooms packaged in nonperforated film and stored at 10 degrees C.
  
  
  CONCLUSIONS
  MAP followed by storage at 4 degrees C or 10 degrees C extends the shelf life by maintaining an acceptable appearance, but allows the growth and survival of L. monocytogenes.
  
  
  CONCLUSIONS
  According to this study additional hurdles must be studied in order to prevent the growth of L. monocytogenes.
authors.affiliation: Departamento de Agricultura y Alimentación, Area de Tecnología de los Alimentos, Universidad de La Rioja, Logroño, Spain. elena.gonzalez@daa.unirioja.es
authors.forename: E
authors.initials: E
authors.lastname: González-Fandos
authors.forename: C
authors.initials: C
authors.lastname: Olarte
authors.forename: M
authors.initials: M
authors.lastname: Giménez
authors.forename: S
authors.initials: S
authors.lastname: Sanz
authors.forename: A
authors.initials: A
authors.lastname: Simón
mesh_terms: D000364:Agaricus / Q000502:physiology*; D001419:Bacteria / Q000302:isolation & purification; D002245:Carbon Dioxide / Q000032:analysis; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000254:growth & development*; D010100:Oxygen / Q000032:analysis; D011143:Polyvinyl Chloride; D013171:Spores, Bacterial / Q000302:isolation & purification; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D011143:Polyvinyl Chloride; D010100:Oxygen