title: Frequency of clonal intraepithelial T lymphocyte proliferations in enteropathy-type intestinal T cell lymphoma, coeliac disease, and refractory sprue.
abstract: BACKGROUND
  Clonal T cell receptor (TCR) gene rearrangements and loss of T cell antigens such as CD8 and TCR-beta in intraepithelial lymphocytes (IELs) may indicate the development of an enteropathy-type intestinal T cell lymphoma (EITCL) in patients with refractory sprue.
  
  
  OBJECTIVE
  To define the diagnostic value of these markers in duodenal biopsies from patients with villous atrophy as a result of various underlying disorders.
  
  
  METHODS
  Duodenal biopsies from eight patients with coeliac disease and five patients with villous atrophy caused by defined disorders were compared with three patients with refractory sprue evolving into overt EITCL, two patients with ulcerative jejunitis, and with eight patients with overt EITCL, for expression of CD3, CD4, CD8, and TCR-beta in IELs using immunohistochemistry and for clonal TCR-gamma gene rearrangements using polymerase chain reaction. In addition, biopsies from six consecutive patients with refractory sprue of uncertain cause were examined.
  
  
  RESULTS
  Clonal TCR-gamma gene rearrangements were found in all resected tumours of patients with EITCL, in 3/8 duodenal biopsies of patients with EITCL, in 2/2 patients with ulcerative jejunitis, in 2/3 patients with refractory sprue evolving into overt EITCL, and in 1/6 patients with refractory sprue. No rearrangements were found in biopsies from patients with refractory sprue caused by defined disorders or those with coeliac disease. Clonality in duodenal biopsies was associated with an abnormal phenotype of IELs in all cases and in all but one case in patients with evidence of underlying coeliac disease. Specificity for detection of an EITCL using immunohistology was 77% for CD8 and for TCR-beta staining, and 100% for detection of a clonal TCR-gamma gene rearrangement. Sensitivity was 62% for staining with CD8 and clonality investigation, while sensitivity reached 100% for TCR-beta staining in all investigated patients with EITCL.
  
  
  CONCLUSIONS
  Clonal proliferations of phenotypically abnormal IELs in refractory sprue represent an early manifestation of EITCL, for which the term "sprue-like intestinal T cell lymphoma" is proposed. This constellation is also found in duodenal biopsies from patients with an overt EITCL and is not related to other sprue syndromes, resulting in a high specificity for detection of an EITCL or refractory sprue evolving into EITCL. Overt EITCL may develop directly from coeliac disease without a precursor lesion (refractory sprue with clonal IELs) being demonstrable in duodenal biopsies or via a "sprue-like intestinal T cell lymphoma". This latter entity is a complication of coeliac disease.
authors.affiliation: Medical Clinic I, Gastroenterology and Infectious Diseases, Universitätsklinikum Benjamin Franklin, Free University of Berlin, Hindenburgdamm 30, 12200 Berlin, Germany. s.daum@ukbf-fu.berlin.de
authors.forename: S
authors.initials: S
authors.lastname: Daum
authors.forename: D
authors.initials: D
authors.lastname: Weiss
authors.forename: M
authors.initials: M
authors.lastname: Hummel
authors.forename: R
authors.initials: R
authors.lastname: Ullrich
authors.forename: W
authors.initials: W
authors.lastname: Heise
authors.forename: H
authors.initials: H
authors.lastname: Stein
authors.forename: E O
authors.initials: EO
authors.lastname: Riecken
authors.forename: H D
authors.initials: HD
authors.lastname: Foss
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016827:CD8 Antigens / Q000032:analysis; D002446:Celiac Disease / Q000276:immunology*; D002455:Cell Division; D002648:Child; D002999:Clone Cells; D004386:Duodenum / Q000276:immunology*; D004751:Enteritis / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D005819:Genetic Markers; D006801:Humans; D007414:Intestinal Neoplasms / Q000276:immunology*; D016399:Lymphoma, T-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000276:immunology; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016827:CD8 Antigens; D005819:Genetic Markers; D016693:Receptors, Antigen, T-Cell, alpha-beta