title: 5'-bis-pyrenylated oligonucleotides display enhanced excimer fluorescence upon hybridization with DNA and RNA.
abstract: A simple one-step procedure was applied for synthesis of oligonucleotide conjugates bearing two pyrene residues at the 5'-phosphate of oligonucleotide. Excimer fluorescence intensity of the conjugates is highly sensitive to duplex formation: binding of the bis-pyrenylated oligonucleotides to their DNA and RNA targets leads 10-fold increase of fluorescence. The data show that excimer fluorescence intensity of the conjugates depends linearly on the concentration of target DNA and permits quantification of DNA in solution.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Russia.
authors.forename: E
authors.initials: E
authors.lastname: Kostenko
authors.forename: M
authors.initials: M
authors.lastname: Dobrikov
authors.forename: N
authors.initials: N
authors.lastname: Komarova
authors.forename: D
authors.initials: D
authors.lastname: Pyshniy
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
authors.forename: M
authors.initials: M
authors.lastname: Zenkova
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA