Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts.
The application of gel electrophoresis and numerical analysis of yeast soluble proteins analysis to the investigation of 12 oral yeast strains belonging to five species is described. It involves one-dimensional electrophoresis of SDS-solubilized whole-cell proteins using different culture media for the cultivation of the cells, integration densitometries in the areas of the gels and percentages of the proteins extraction. These extracts were prepared from four isolates of Candida albicans, two of C. tropicalis, C. guilliermondii, C. parapsilosis and C. krusei. The extracts from whole-cells proteins using different culture media for the cultivation of the cells were fractionated by slab electrophoresis using a discontinuous buffer system. The corresponding patterns showed at least 36 polypeptides in the range of 14.4-200 kDa. Different isolates of each species were clearly different in each of the five species. The data obtained suggest that different nutritional compositions led to the expression of different proteins derived from alternatives metabolic pathways expressed by the electrophoretic profiles. The construction of a database of protein fingerprints and numerical analysis based on such data, may have some implications in the classification and identification of such species with epidemiological, ecological and taxonomic purposes. A well defined or synthetic culture media seems to be much properly.
Höfling
J F
JF
Laboratório de Microbiologia e Imunologia, Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Unicamp, SP, Brazil. hofling@fop.unicamp.br
Rosa
E A
EA
Pereira
C V
CV
Boriollo
M F
MF
Rodrigues
J A
JA
D002175:Candida / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D009716:Numerical Analysis, Computer-Assisted
D003470:Culture Media; D005656:Fungal Proteins