title: An alternative solid phase peptide fragment condensation protocol with improved efficiency.
abstract: The success of solid phase peptide synthesis is often limited by the aggregation of the growing peptide chains on the resin. Working from the results of a study of model coupling reactions in solution between Z-Gly-Phe-OH and H-Phe-OBzl, we have achieved higher efficiency in the repetitive solid phase fragment condensation of VGVAPG, in a 3:1 chloroform-phenol solvent system, using diisopropylcarbodiimide (DIC) as coupling agent, and a combination of 3-hydroxy-3,4-dihydro-4-oxo-1,2,3-benzotriazine (HODhbt) and its tetrabutyl ammonium salt as additive, than in DMF with DIC and HODhbt alone.
authors.affiliation: Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Lorond University, Budapest.
authors.forename: N
authors.initials: N
authors.lastname: Mihala
authors.forename: J
authors.initials: J
authors.lastname: Bódi
authors.forename: A
authors.initials: A
authors.lastname: Gömöry
authors.forename: H
authors.initials: H
authors.lastname: Süli-Vargha
mesh_terms: D000595:Amino Acid Sequence; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid; D008722:Methods; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D019800:Phenol; D012996:Solutions
chemical_list: D010446:Peptide Fragments; D012996:Solutions; D019800:Phenol; D002725:Chloroform