title: A method for UV-bonding in the fabrication of glass electrophoretic microchips.
abstract: This paper presents an approach for the development of methodologies amenable to simple and inexpensive microchip fabrication, potentially applicable to dissimilar materials bonding and chip integration. The method involves a UV-curable glue that can be used for glass microchip fabrication bonding at room temperature. This involves nothing more than fabrication of glue "guide channels" into the microchip architecture that upon exposure to the appropriate UV light source, bonds the etched plate and cover plate together. The microchip performance was verified by capillary zone electrophoresis (CZE) of small fluorescent molecules with no microchannel surface modification carried out, as well as with a DNA fragment separation following surface modification. The performance of these UV-bonded electrophoretic microchips indicates that this method may provide an alternative to high temperature bonding.
authors.affiliation: Department of Chemistry University of Virginia Health Sciences Center, Charlottesville 22904, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.forename: J C
authors.initials: JC
authors.lastname: Sanders
authors.forename: C
authors.initials: C
authors.lastname: Dunsmor
authors.forename: H
authors.initials: H
authors.lastname: Ahmadzadeh
authors.forename: J P
authors.initials: JP
authors.lastname: Landers
mesh_terms: D000269:Adhesives / Q000528:radiation effects*; D053938:DNA Fragmentation; D004274:DNA, Recombinant / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D019793:Fluorescein / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D005470:Fluorometry / Q000295:instrumentation; D005898:Glass; D006358:Hot Temperature; D008832:Microchemistry / Q000295:instrumentation*; D014466:Ultraviolet Rays
chemical_list: D000269:Adhesives; D004274:DNA, Recombinant; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate; D019793:Fluorescein