Automated strong cation exchange extraction of fatty acid esters of 3-(N-phenylamino)-1,2-propanediol from oil samples for routine quantification by HPLC-APCI/MS/MS.
Fatty acid esters of 3-(N-phenylamino)-1,2-propanediol are currently considered the best chemical markers of toxic oils related to the Spanish toxic oil syndrome. Recent research in this area has undertaken the exhaustive and quantitative characterization of these compounds in oils collected during the epidemic outbreak. Current methods developed in this laboratory are based on solid phase extraction (SPE) using SCX cartridges followed by HPLC-APCI/MS/MS quantification. To circumvent the long and tedious extraction procedure, the SPE protocol was adapted for automatic extraction and the problems derived from the use of the immiscible solvents required for the SCX extraction were solved. Linearity of the analytical method was found in the same range as for the manual method. Extraction recoveries were 87 and 75% for 2-hydroxy-3-(N-phenylamino)propyl linoleate and 2-(linoleyloxy)-3-(N-phenylamino)propyl linoleate, respectively, and the corresponding coefficients of variation were approximately 1%, greatly improving reproducibility over manual procedures.
Calaf
R E
RE
Structural and Biological Mass Spectrometry Unit, Department of Medical Bioanalysis, IIBB-CSIC, IDIBAPS, Rosellón 161, 08036 Barcelona, Spain.
Peña
J
J
Paytubi
S
S
Carrascal
M
M
Posada
M
M
Gelpi
E
E
Abian
J
J
D002411:Cation Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D019787:Linoleic Acid / Q000302:isolation & purification*; D008041:Linoleic Acids; D013058:Mass Spectrometry / Q000379:methods*
C443340:2-(linoleyloxy)-3-(N-phenylamino)propyl linoleate; C443339:2-hydroxy-3-(N-phenylamino)propyl linoleate; D002411:Cation Exchange Resins; D008041:Linoleic Acids; D019787:Linoleic Acid