title: A monoclonal antibody that recognizes a potential coeliac-toxic repetitive pentapeptide epitope in gliadins.
abstract: OBJECTIVE
  Antibodies that detect coeliac-toxic prolamins from wheat, barley and rye are important tools for controlling the diet of coeliac disease patients. Recently, a monoclonal antibody R5 that recognizes wheat gliadin, barley hordein and rye secalin equally was described. In this study, the epitope recognized by R5 was investigated.
  
  
  METHODS
  Both a phage-displayed heptapeptide library and overlapping peptides spanning the sequence of alpha- and gamma-type gliadins (pepscan) were screened for binding of R5.
  
  
  RESULTS
  Both techniques yielded comparable pentapeptide consensus sequences (phage display QXPW/FP; pepscan QQPFP). According to recent observations, this peptide stretch may be of key importance in the pathogenicity of coeliac disease. This sequence occurs repetitively in prolamins (in gamma- and omega-type prolamins more frequently than in alpha-type prolamins) together with several homologous peptide stretches, which are recognized less strongly.
  
  
  CONCLUSIONS
  R5 seems to be a good candidate for the specific detection of putative coeliac disease-active sequences in prolamins and thus represents a valuable tool for the quality control of gluten-free food.
authors.affiliation: Department of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Leipzig, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Osman
authors.forename: H H
authors.initials: HH
authors.lastname: Uhlig
authors.forename: I
authors.initials: I
authors.lastname: Valdes
authors.forename: M
authors.initials: M
authors.lastname: Amin
authors.forename: E
authors.initials: E
authors.lastname: Méndez
authors.forename: T
authors.initials: T
authors.lastname: Mothes
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005504:Food Analysis; D005903:Gliadin / Q000235:genetics* / Q000276:immunology; D005983:Glutens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005983:Glutens; D005903:Gliadin