Isolation and sequence analysis of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol & Gerd.) Gerdemann & Trappe 3-phosphoglycerate kinase (PGK) gene promoter region.
The Glomus mosseae 3-phosphoglycerate kinase (GmPGK) gene promoter has been isolated from a phage genomic library and represents one of the few promoter elements to be isolated and analysed from these symbiotic fungi. The analysis revealed the presence of several motifs which are found in the promoter region of other fungal PGK genes. In particular, DNA sequences homologous to segments of the S. cerevisiae and Rhizopus niveus upstream activating elements (UAS). The importance of these UAS sequences in regulating carbon source in PGK genes is known and the presence of two carbon source regulated UAS sequences in the GmPGK gene promoter and its role in the biology of AM fungi is discussed briefly.
Harrier
L A
LA
Plant Science Division, Kings Buildings, Scottish Agricultural College, West Mains Road, Edinburgh, Scotland, UK EH9 3JG. L.Harrier@ed.sac.ac.uk
D051696:Activating Transcription Factors; D001483:Base Sequence; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D016384:Consensus Sequence; D018076:DNA, Complementary; D005057:Eukaryotic Cells; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism
D051696:Activating Transcription Factors; D001798:Blood Proteins; D018076:DNA, Complementary; D014157:Transcription Factors; D010735:Phosphoglycerate Kinase