title: Studies towards the detection and identification of sesquiterpene pyridine alkaloids in Peritassa campestris by mass spectrometry.
abstract: Sesquiterpene pyridine alkaloids were analysed in Peritassa campestris by mass spectrometric techniques such as ESI-MSMS and GCMS. Ten alkaloids previously isolated from this plant and fully identified by other physical methods, including NMR spectroscopy and X-ray diffraction, were carefully studied by MS. It was observed that the low mass ions detected at m/z 178 and 206 in both MS technique tested were characteristic of evoninic and wilfordic acids, which are part of the macrolactone in these sesquiterpene alkaloids. The intensity of a fragment ion detected at m/z 93 in CAD, and especially in EI, spectra was found to be diagnostic in distinguishing between evoninoates and wilfordates. Running parent/daughter or GCMS experiments enabled these substances to be detected in crude fractions of P. campestris. Parent ion scans of m/z 206 were very as a first analysis of an alkaloid mixture.
authors.affiliation: Department of Chemistry, Universidade Federal de São Carlos, Caixa Postal 676, São Carlos, SP, Brazil. edson@dq.ufscar.br
authors.forename: E
authors.initials: E
authors.lastname: Rodrigues-Fo
authors.forename: F A
authors.initials: FA
authors.lastname: Barros
authors.forename: J B
authors.initials: JB
authors.lastname: Fernandes
authors.forename: M F
authors.initials: MF
authors.lastname: da Silva
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D011725:Pyridines / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D000470:Alkaloids; D011725:Pyridines; D012717:Sesquiterpenes