NMR-based identification of cell wall anionic polymers of Spirilliplanes yamanashiensis VKM Ac-1993(T).
The cell wall of Spirilliplanes yamanashiensis VKM Ac-1993(T) contains four anionic polymers, viz., three teichoic acids and a sugar-1-phosphate polymer. The following are the structures of the teichoic acids: poly[-6-beta-D-glucopyranosyl-(1-->2)-glycerol phosphate] (PI), 1,3-poly(glycerol phosphate) bearing N-acetyl-alpha-D-glucosamine residues at O-2 (70%) (PII), and poly[-6-N-acetyl-alpha-D-glucosaminyl-(1-->2)-glycerol phosphate] (PIII). The repeating unit of the fourth polymer (PIV) has the structure of -6-alpha-D-GlcpNAc-(1-->6)-alpha-D-GlcpNAc-1-P- with a 3-O-methyl-alpha-D-mannopyranosyl residues at position 3 of some 6-phosphorylated N-acetylglucosamine residues (50%). Polymers PI, PIII and PIV have not hitherto been found in prokaryotic cell walls.
Shashkov
A S
AS
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117334, Russia.
Streshinskaya
G M
GM
Evtushenko
L I
LI
Naumova
I B
IB
D000192:Actinomycetales / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008848:Micromonosporaceae / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000737:chemistry; D013403:Sugar Phosphates / Q000737:chemistry; D013682:Teichoic Acids / Q000737:chemistry
D011135:Polysaccharides, Bacterial; D013403:Sugar Phosphates; D013682:Teichoic Acids