[Serum beta-glucuronidase activity in polytrauma patients and in centrifuged autopsy blood samples].
The serum activity of beta-glucuronidase was investigated in 58 patients after severe trauma as well as in 43 autopsy cases. In 10 cases the enzyme activities in postmortem blood samples from the femoral vein were compared to those present in the correspondent heart blood samples. An elevated activity of beta-glucuronidase was observed in 14% of the patients within the first 36 h after severe trauma increasing to 62% in blood samples collected later on. The activity of beta-glucuronidase in the heart blood samples was always higher than in the corresponding sample from the femoral vein. In cases of prolonged post-mortem interval an elevated activity might have been due to bacterial contamination. In postmortal blood samples from the femoral vein an elevated enzyme activity was found in 70% of the study material. The results of the preliminary study on the activity of beta-glucuronidase in blood samples frequent in forensic routine work indicated that an elevated enzyme activity might be present for the following scenery: after severe trauma, in alcohol/drug abuse, presence of putridity/autolysis, presence of inflammatory processes, in diabetes as well as in carcinoma diseases. The significance of elevated beta-glucuronidase activity concerning alterations of unconjugated drug concentration due to in vitro cleavage of O-glucuronides should be investigated.
Pötsch
L
L
Institut für Rechtsmedizin, Universität Mainz.
Skopp
G
G
D001344:Autopsy / Q000331:legislation & jurisprudence*; D005966:Glucuronidase / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009104:Multiple Trauma / Q000175:diagnosis* / Q000201:enzymology / Q000473:pathology; D011180:Postmortem Changes; D012016:Reference Values
D005966:Glucuronidase