[Factor V Leiden and PTG20210A gene mutation in patients with venous thrombosis and healthy blood donors].
OBJECTIVE
To evaluate the incidence of factor V Leiden and prothrombin gene G20210A mutation in patients with venous thrombosis and healthy volunteers.


METHODS
Factor V Leiden and prothrombin gene G20210A mutation were analysed in 97 cases of venous thrombosis and 100 healthy volunteers with the methods of one-step PCR-RFLP.


RESULTS
PCR products for the factor V gene (175 bp) and for the prothrombin gene (118 bp) were identified to 157 bp and 98 bp fragments by electrophoresis after Taq I treatment. No factor V Leiden and prothrombin gene G20210A mutation were found in either group.


CONCLUSIONS
The low incidence of factor V Leiden and prothrombin gene G20210A mutation suggest that they are not the major genetic risk factors for thrombophilia in the Chinese.
Bai
C
C
PUMC Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.
Pan
J
J
Li
X
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*; D011655:Pulmonary Embolism / Q000209:etiology; D020246:Venous Thrombosis / Q000150:complications / Q000235:genetics*
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin