title: Systemic antibodies to Clostridium botulinum type C: do they protect horses from grass sickness (dysautonomia)?
abstract: The aetiology of equine grass sickness (EGS) is still unknown. There is increasing evidence that toxicoinfection with Clostridium botulinum type C is involved. Epidemiological evidence shows that resistance to EGS can occur in older horses and those that have been on a particular pasture for longer or have been in prior contact with the disease. This resistance may be in the form of an immune response to the aetiological agent. Levels of systemic antibodies to the surface antigens of C. botulinum type C (using the closely related and safe C. novyi type A as a phenotypic marker) and to the botulinum type C neurotoxin (BoNT/C) were investigated in horses with and without EGS. Horses with grass sickness were found to have significantly lower levels of systemic IgG to both surface antigens and BoNT/C. Horses with low levels of systemic immunity to these antigens may be more susceptible to developing EGS. There were no significant differences in antibody levels between the different categories of EGS, suggesting systemic immunity to C. botulinum type C does not play a significant role in influencing the severity of the disease. However, horses that had been in contact with EGS or that were grazing land where it had occurred frequently in the past had significantly higher antibody levels to these antigens. These horses may have been exposed to subclinical doses of C. botulinum type C and BoNT/C, resulting in the production of a protective immune response against the putative aetiological agent. This finding is of potential significance for the prospect of prevention of EGS by vaccination against C. botulinum type C.
authors.affiliation: Medical Microbiology, University of Edinburgh Medical School, UK.
authors.forename: L C
authors.initials: LC
authors.lastname: Hunter
authors.forename: I R
authors.initials: IR
authors.lastname: Poxton
mesh_terms: D000367:Age Factors; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000954:Antigens, Surface / Q000097:blood; D001342:Autonomic Nervous System Diseases / Q000382:microbiology / Q000662:veterinary*; D001905:Botulinum Toxins / Q000276:immunology; D003014:Clostridium botulinum / Q000276:immunology* / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006734:Horse Diseases / Q000209:etiology* / Q000382:microbiology / Q000517:prevention & control; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood; D010939:Plant Poisoning / Q000517:prevention & control / Q000662:veterinary*; D006109:Poaceae / Q000506:poisoning*
chemical_list: D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D007074:Immunoglobulin G; D001905:Botulinum Toxins; C096324:botulinum toxin type C