Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association.
The RNase MRP and RNase P ribonucleoprotein particles both function as endoribonucleases, have a similar RNA component, and share several protein subunits. RNase MRP has been implicated in pre-rRNA processing and mitochondrial DNA replication, whereas RNase P functions in pre-tRNA processing. Both RNase MRP and RNase P accumulate in the nucleolus of eukaryotic cells. In this report we show that for three protein subunits of the RNase MRP complex (hPop1, hPop4, and Rpp38) basic domains are responsible for their nucleolar accumulation and that they are able to accumulate in the nucleolus independently of their association with the RNase MRP and RNase P complexes. We also show that certain mutants of hPop4 accumulate in the Cajal bodies, suggesting that hPop4 traverses through these bodies to the nucleolus. Furthermore, we characterized a deletion mutant of Rpp38 that preferentially associates with the RNase MRP complex, giving a first clue about the difference in protein composition of the human RNase MRP and RNase P complexes. On the basis of all available data on nucleolar localization sequences, we hypothesize that nucleolar accumulation of proteins containing basic domains proceeds by diffusion and retention rather than by an active transport process. The existence of nucleolar localization sequences is discussed.
van Eenennaam
H
H
Department of Biochemistry, University of Nijmegen, NL-6500 HB Nijmegen, The Netherlands.
van der Heijden
A
A
Janssen
R J
RJ
van Venrooij
W J
WJ
Pruijn
G J
GJ
D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002352:Carrier Proteins; D002466:Cell Nucleolus / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism*; D006801:Humans; D016337:RNA, Catalytic / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P; D012260:Ribonucleases; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D051017:Apoptosis Regulatory Proteins; D001324:Autoantigens; D002352:Carrier Proteins; C490350:POP1 protein, human; D016337:RNA, Catalytic; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D004720:Endonucleases; D004722:Endoribonucleases; D012260:Ribonucleases; C058330:mitochondrial RNA-processing endoribonuclease; C105253:POP4 protein, human; C468368:RPP14 protein, human; C104645:RPP38 protein, human; D043262:Ribonuclease P