Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices.
A multichannel electrospray ionization (ESI) emitter was fabricated as part of a poly(dimethylsiloxane) (PDMS) microfluidic device using a three-layer photoresist process which also produces a self-alignment system to make a bonding between the top and bottom PDMS parts. The prototype device (2 cm high x 5 cm wide x 5 cm long) had 16-channels (30 microm wide x 50 microm deep) with emitters of 1 mm length and 60 degrees point angle. The PDMS emitter tips enabled interfacing the device to ESI-mass spectrometry; a stable electrospray from the tips was performed with limits of detection under 1 microM for reference peptides (adrenocorticotropic hormone fragment 1-17, angiotensin I and III).
Kim
J S
JS
Department of Pharmacology, Medical University of South Carolina, Charleston 29425, USA.
Knapp
D R
DR
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000803:Angiotensin I / Q000032:analysis; D000805:Angiotensin III / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D010446:Peptide Fragments / Q000032:analysis; D012212:Rheology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
D010446:Peptide Fragments; D000805:Angiotensin III; D000324:Adrenocorticotropic Hormone; D000803:Angiotensin I; C028238:ACTH (1-17)