title: Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli.
abstract: LCI is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues with a molecular weight of 5468 Da. Using bioengineering, LCI was expressed in Escherichia coli DH5alpha with recombinant plasmid pBVAB16. It was crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 29.30, c = 187.09 A, and diffracts to 2.44 A. A set of diffraction data to 2.8 A was collected.
authors.affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
authors.forename: J P
authors.initials: JP
authors.lastname: Zhu
authors.forename: B Z
authors.initials: BZ
authors.lastname: Chen
authors.forename: W B
authors.initials: WB
authors.lastname: Gong
authors.forename: Y H
authors.initials: YH
authors.lastname: Liang
authors.forename: H C
authors.initials: HC
authors.lastname: Wang
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.forename: Z L
authors.initials: ZL
authors.lastname: Chen
authors.forename: G Y
authors.initials: GY
authors.lastname: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides; D001412:Bacillus subtilis / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D010455:Peptides; D011487:Protein Conformation
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C444362:antibacterial polypeptide LCI