Automated ribotyping provides rapid phylogenetic subgroup affiliation of clinical extraintestinal pathogenic Escherichia coli strains.
Using the automated Riboprinter system, we have initiated the construction of an electronic Riboprint database composed of 72 ECOR reference strains and 15 archetypal virulent strains in order to provide a new simple molecular characterization method. More than 90% of the ECOR strains clustered in their original phylogenetic group. All but one of the archetypal virulent strains had a profile identical to that of one of the ECOR strains and could be easily affiliated with a phylogenetic group. This method appears to be an accurate and practical tool especially for investigating the genetic relationship between clinical extraintestinal pathogenic strains and B2 subgroup ECOR strains or archetypal pathotype strains.
Clermont
O
O
Laboratoire d'Etudes de Génétique Bactérienne dans les Infections de l'Enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France.
Cordevant
C
C
Bonacorsi
S
S
Marecat
A
A
Lange
M
M
Bingen
E
E
D016470:Bacteremia / Q000382:microbiology; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D020814:Meningitis, Escherichia coli / Q000382:microbiology; D010802:Phylogeny; D012015:Reference Standards; D021521:Ribotyping / Q000379:methods*; D013997:Time Factors; D014552:Urinary Tract Infections / Q000382:microbiology; D014774:Virulence