Molecular epidemiology of tuberculosis among eight hospitals in New York City, 1996-1997.
OBJECTIVE
To determine the molecular epidemiology of tuberculosis isolated from patients cared for at eight hospitals scattered throughout New York City.


METHODS
Cases of tuberculosis occurring in 1996 and 1997 at collaborating hospitals were identified, and demographic data were extracted from patient charts. All available isolates were analyzed by IS6110 for genetic relatedness. The molecular fingerprints were compared both to each other and to the larger repository of strains from New York City developed and maintained at the Public Health Research Institute.


RESULTS
One hundred and eighty cases were fully characterized. Compared with New York City cases, study patients were more likely to be Asian and less likely to be non-Hispanic blacks. Overall, 97 (54%) of the cases were clustered with respect to other study strains or with respect to the other New York City isolates. Clustered strains were significantly more likely to be from non-Hispanic blacks or patients born in the United States. The largest cluster (n = 17) was the "W" strain previously associated with an outbreak of multidrug-resistant tuberculosis in New York City. In the current study, the majority of W strain isolates were fully drug-susceptible.


CONCLUSIONS
High rates of genetically related tuberculosis continue to occur among patients in New York City, in spite of improved control of nosocomial outbreaks and dramatic decreases in the overall case rates. The use of molecular techniques to suggest patterns of transmission has become essential in developing and assessing routine tuberculosis control strategies.
Magnani
J
J
New York Hospital-Cornell Medical Center, New York, NY, USA.
Mathema
B
B
Berger
J
J
Brown
S
S
David
M
M
Glatt
A
A
Pujol
F
F
Segal-Maurer
S
S
Riley
L W
LW
Kreiswirth
B N
BN
Sepkowitz
K A
KA
D000328:Adult; D000368:Aged; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification; D009519:New York City / Q000453:epidemiology; D013997:Time Factors; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology