Kinetics of antigen-induced phenotypic and functional maturation of human monocyte-derived dendritic cells.
Dendritic cells (DCs), a critical component of innate immunity, are the most potent APCs. When DCs mature, they can elicit strong T cell responses. We studied the kinetics of Ag-induced phenotypic and functional maturation of human monocyte-derived DCs using an in vitro T cell-independent culture system. With this model, we herein show that an Ag that has recently or repetitively been exposed ("exposed Ag") rapidly induces a high level of maturation; however, an Ag that has never or only remotely been exposed ("unexposed Ag") slowly induces a low level of maturation. The kinetics of Ag-induced maturation of DCs possibly implies a novel mechanism for immunological memory that would provide maximal host protection from repetitively invading pathogens in the environment.
Hsieh
S M
SM
Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. hsmaids@hotmail.com
Pan
S C
SC
Hung
C C
CC
Tsai
H C
HC
Chen
M Y
MY
Lee
C N
CN
Chang
S C
SC
D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis; D000955:Antigens, T-Independent / Q000502:physiology*; D000956:Antigens, Viral / Q000502:physiology; D018122:B7-1 Antigen / Q000032:analysis / Q000096:biosynthesis; D051940:B7-2 Antigen; D015415:Biomarkers / Q000032:analysis; D019013:CD40 Antigens / Q000032:analysis / Q000096:biosynthesis; D002453:Cell Cycle / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D006684:HLA-DR Antigens / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis; D018448:Models, Immunological; D009000:Monocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors
D015703:Antigens, CD; D000955:Antigens, T-Independent; D000956:Antigens, Viral; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D019013:CD40 Antigens; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins