[Adenovirus-transferred antisense c-myc selectively induces tumor cell cycle arrest and apoptosis].
OBJECTIVE
To construct the recombinant adenovirus encoding antisense c-myc fragment and to investigate its effect on tumor cell proliferation and apoptosis.


METHODS
The shuttle plasmid encoding antisense c-myc was constructed by cloning c-myc cDNA fragment in the reverse direction into the pAdCMV. Then the plasmid pJM17 and the shuttle plasmid were co-transferred into 293 cells with liposome for homologous recombination to acquire recombinant adenovirus. The cell cycle, apoptosis, and the expression of related genes of the in vitro transferred human adenocarcinoma cell lines GLC-82 and SPC-A-1, as well as the human embryonic diploid lung cell line 2BS were studied. The effects on colony formation and treating effect on transplanted tumor in nude mice were also studied.


RESULTS
The recombinant adenovirus encoding antisense c-myc fragment was obtained with the titer of 3.3 x 10(10) pfu/ml. RT-PCR and Western blot showed that the expression of c-myc was reduced 3-5 days after infection. Simultaneously, obvious G1 arrest and apoptosis as well as the alteration of cyclin D1, bcl-2 and bax gene expression were observed in the infected tumor cells. The infected 2BS cells showed no such changes. The colony formation ability in vitro and the growth of the hetero-transplanted tumor in nude mice were also reduced.


CONCLUSIONS
The reduced expression of c-myc gene could induce human adenocarcinoma cells tumor-cell-specific G1 arrest and apoptosis, while it has no effect on normal cell.
Wei
Y
Y
National Lab of Molecular Oncology, Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Lin
C
C
Zhang
X
X
D000230:Adenocarcinoma / Q000473:pathology*; D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D017209:Apoptosis; D016373:DNA, Antisense; D016193:G1 Phase; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D016271:Proto-Oncogene Proteins c-myc