title: The nef Gene of SIVmac239 Is Necessary for Efficient Growth in H9 Cells.
abstract: AIDS viruses require an intact functional nef gene in order to induce disease. The nonpathogenic molecular cloned virus SIVmac239nef-deletion encodes a truncated nef gene. This attenuated reading frame is expressed both in vitro and in a virus-infected animal in vivo. Encoding the first 58 amino acids of Nef, the reading frame retained its ability to down-modulate CD4 from the surface of T cells. CD4-down-modulated stable cell lines expressing full-length and truncated nef genes were significantly less infected by SIV. SIVmac239nef-open and SIVmacnef-deletion encoding a truncated nef clearly differed in replication kinetics in H9 cells and H9-derived cell lines. SIVmac239nef-deletion replication was delayed in H9. Copyright 1996 S. Karger AG, Basel
authors.affiliation: Department of Molecular Biology, Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA.
authors.forename: J.R.
authors.initials: JR
authors.lastname: Salkowitz
authors.forename: B.K.
authors.initials: BK
authors.lastname: Chakrabarti
authors.forename: B.
authors.initials: B
authors.lastname: Yen-Lieberman
authors.forename: C.
authors.initials: C
authors.lastname: Starkey
authors.forename: T.
authors.initials: T
authors.lastname: Bendele
authors.forename: H.W.
authors.initials: HW
authors.lastname: Kestler