[The ultrastructure of bone morphogenetic protein 2 gene transfected human periodontal ligament fibroblasts].
OBJECTIVE
To observe the ultrastructure of bone morphogenetic protein 2 (BMP-2) gene transfected human periodontal ligament fibroblasts (HPDLFs).


METHODS
An expression vector for BMP-2 was transfected into HPDLFs by using Lipofect AMINE. The stable expression of BMP-2 was determined by in situ hybridization and immunohistochemistry. The ultrastructure of the transfected cells was examined by transmission electron microscope.


RESULTS
The results showed that the BMP-2 gene transfected cells showed expansive endoplasmic reticulum, incremental myelin sheath-like figures and matrix vesicles. The collagenous fibrillae was distributed widely intercellular.


CONCLUSIONS
The results suggests that BMP-2 gene transfection tends to accelerate HPDLFs into osteoblast-like cells.
Si
X
X
Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Liu
Z
Z
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D009186:Myelin Sheath / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology*; D014162:Transfection; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D016212:Transforming Growth Factor beta