title: Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR.
abstract: OBJECTIVE
  To study the incidence of Shiga-toxigenic Escherichia coli (STEC) in seafoods from India.
  
  
  RESULTS
  Escherichia coli isolated from various seafoods such as fresh fish, clams and water were screened for the presence of stx, hlyA and rfbO157 genes by PCR; 5% of clams and 3% of fresh fish samples were positive for non-O157 STEC.
  
  
  CONCLUSIONS
  STEC is prevalent in seafoods in India, and non-O157 serotype is more common.
  
  
  CONCLUSIONS
  Seafood could be a vehicle for transmission of STEC even in tropical countries.
authors.affiliation: Department of Fishery Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore-575 002, India.
authors.forename: H
authors.initials: H
authors.lastname: Sanath Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Otta
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
authors.forename: I
authors.initials: I
authors.lastname: Karunasagar
mesh_terms: D004768:Enterotoxins / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D019453:Escherichia coli O157 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics; D007194:India; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017747:Seafood / Q000382:microbiology*; D022603:Shiga Toxins / Q000032:analysis*; D014774:Virulence
chemical_list: D004768:Enterotoxins; D022603:Shiga Toxins