title: [Regulation of gene co-expression after bicistronic retrovirus-mediated gene transfer into human cord blood CD34+ cells].
abstract: OBJECTIVE
  To compare internal SV40 early promoter with IRES of the in regulatory effect on gene co-expression after retrovirus-mediated gene transfer into human cord blood CD34+ cells.
  
  
  METHODS
  Based on pLXSN, vectors pLESN and pLEIN carrying EGFP and neo gene, distinguished by the in sequences between two, genes, were constructed. After liposome-mediated gene transfer into packaging cells, high-titre virus supernatant was obtained. After supernatant infection in vitro, transduced cells were cultured in CFC assay.
  
  
  RESULTS
  FACS analysis demonstrated that 10% of human cord blood CD34+ cell were infected in vitro. All of G418 resistant colonies demonstrated green fluorescence in LEIN group, while only 41.1% in LESN group.
  
  
  CONCLUSIONS
  IRES is superior to SV40 promoter for the guarantee of co-expression in bicistronic retrovirus-mediated gene therapy in human cord blood CD34+ cells.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Tong
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D013539:Simian virus 40 / Q000235:genetics
chemical_list: D018952:Antigens, CD34