title: The effect of mosquito passage on the La Crosse virus genotype.
abstract: The genetic consequences of passing three different strains of La Crosse (LAC) virus orally and transovarially in Aedes triseriatus mosquitoes were examined. Two of the LAC strains (WT LAC and LAC ORI) had been passaged numerous times in cell culture; the third strain (SM1-78) had been passaged only once in suckling mice. Genetic changes were monitored in three regions of the LAC genome after oral infection and dissemination in the mosquito, and transovarial transmission (TOT) of the virus to progeny. Sequence analyses were used to characterize the genetic changes occurring in regions of G1, G2 and N open reading frames (ORFs) during passage. Only one mutation was detected in the G1 ORF of SM1-78 virus after mosquito passage; however, numerous nucleotide and amino acid substitutions were detected in the G1 ORF of WT LAC and LAC ORI (cell culture-adapted viruses). In contrast to G1, the N and G2 ORF sequences examined were stable. Mutations introduced into viral genomes during replication in parental mosquitoes were expressed in progeny mosquitoes following TOT. Genetic diversity of virus populations from a single mosquito was examined by single-strand conformation polymorphisms analysis of the variable region of glycoprotein G1. LAC virus RNA genotype diversity was greatest in virus that infected and replicated in the midgut, and declined as virus disseminated from the midgut and infected ovaries and salivary glands.
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Monica K
authors.initials: MK
authors.lastname: Borucki
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Kempf
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Carol D
authors.initials: CD
authors.lastname: Blair
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D003001:Cloning, Molecular; D009033:Culicidae / Q000821:virology*; D005260:Female; D005838:Genotype; D007422:Intestines / Q000821:virology; D018062:La Crosse virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010053:Ovary / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication / Q000235:genetics
chemical_list: C400973:ORF3 protein, Hepatitis E virus; D014764:Viral Proteins