The sow endometrium at different stages of the oestrous cycle: immunohistochemical study on the distribution of SWC3-expressing cells (granulocytes, monocytes and macrophages).
Uterine samples from sows taken immediately after slaughter at late di-oestrus, pro-oestrus, oestrus, early di-oestrus and di-ocstrus, were analysed by immunohistochemistry with an avidinbiotin-peroxidase method using a monoclonal antibody (anti-SWC3) to granulocyte, monocyte and macrophage populations. The endometrium was then examined by light microscopy. In the surface and glandular epithelium, the largest numbers of SWC3-expressing cells (P < or = 0.01 and P < or = 0.05) were found at oestrus, and at pro-oestrus and oestrus, respectively. The numbers of SWC3-expressing cells in the epithelium were positively correlated with the plasma levels of oestradiol-17beta. In the connective tissue of the subepithelial and glandular layers, no significant effect of the oestrous Cycle stage was found on the number of SWC3-expressing cells. The present study showed a variation in the distribution of SWC3-expressing cells in the sow endometrium, especially in the surface and glandular epithelium, during different stages of the oestrous cycle.
Kaeoket
K
K
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. kampon.kaeoket@og.slu.se
Dalin
A M
AM
Magnusson
U
U
Persson
E
E
D000818:Animals; D004717:Endometrium / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000276:immunology / Q000502:physiology*; D005260:Female; D006098:Granulocytes / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D011374:Progesterone / Q000097:blood; D013552:Swine / Q000276:immunology / Q000502:physiology*
D011374:Progesterone; D004958:Estradiol