5,7-Dihydroxytryptamine: regional brain concentrations following intraventricular administration to rats.
The concentrations of 5,7-dihydroxytryptamine (5,7-DHT) and serotonin (5-HT) were measured in brainstem, hypothalamus and cerebral cortex 0, 2, 6, 12, and 24 hours following the bilateral, lateral ventricular injection of 5,7-DHT (100 microg/each ventricle) into adult male rats. At 6 hours, 5,7-DHT levels had decreased 99% from 0 hr values in all brain regions. Thereafter, 5,7-DHT levels continued to decline in cortex, but not in hypothalamus or brainstem; at 24 hr, but not 48 hr, 5,7-DHT peaks were still measurable in each brain region examined. Serotonin levels in all three regions also fell markedly by 2-6 hours after 5,7-DHT administration. At 24 hours, hypothalamus and brainstem 5HT levels had declined >70% and cerebral cortex approximately 50% below control values. The relevance of these findings to the protective action of monoamine reuptake blockers is discussed.
Choi
S J
SJ
Department of Psychiatry, Western Psychiatric Institute & Clinic, Pittsburgh, PA 15213, USA. choisj@pitt.edu
Patil
V
V
Fernstrom
J D
JD
D015116:5,7-Dihydroxytryptamine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002552:Cerebral Ventricles / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D007276:Injections, Intraventricular; D007700:Kinetics; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
D015116:5,7-Dihydroxytryptamine; D012701:Serotonin