Gene expression profiling of aging using DNA microarrays.
We have previously employed high density oligonucleotide arrays representing thousands of genes to determine the gene expression profile of the aging process in skeletal muscle (gastrocnemius) and brain (cerebellum and neocortex) of male C57BL/6 mice. Specific gene expression profiles are associated with the aging process of individual organs, and caloric restriction can prevent or retard the establishment of these gene expression alterations. The use of DNA microarrays may provide a new tool to measure biological age on a tissue-specific basis and to evaluate at the molecular level the efficacy of interventions designed to retard the aging process.
Weindruch
Richard
R
Department of Medicine, University of Wisconsin-Madison and Veterans Administration Hospital, Geriatric Research, Education and Clinical Center, Madison, WI 53705, USA.
Kayo
Tsuyoshi
T
Lee
Cheol Koo
CK
Prolla
Tomas A
TA
D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001921:Brain; D002149:Energy Intake; D015870:Gene Expression; D020869:Gene Expression Profiling; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011506:Proteins / Q000378:metabolism; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D011506:Proteins