Determination of marker constituents from Cissus quadrangularis Linn. and their quantitation by HPTLC and HPLC.
Four marker constituents, namely, onocer-7-ene-3 alpha, 21 beta-diol, delta-amyrin, delta-amyrone and 3,3',4,4'-tetrahydroxybiphenyl of an Ayurvedic crude drug Cissus quadrangularis Linn. are defined for standardisation purposes. 3,3',4,4'-Tetrahydroxybiphenyl has been isolated for the first time from this drug. The contents of the marker constituents were quantitatively determined by HPTLC and HPLC methods in samples collected from five different geographic zones of India.
Mehta
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Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Mohali, Near Chandigarh 160062, India.
Kaur
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N
Bhutani
K K
KK
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D015203:Reproducibility of Results; D027844:Vitaceae / Q000737:chemistry*