[In vitro proliferation and differentiation of bone marrow stem cells of aplastic anemia patients].
OBJECTIVE
To investigate the quality of bone marrow stem cells from aplastic anemia patients and their in vitro responses to hemopoietic growth factors(HGF).


METHODS
CD34 positive bone marrow cells from 10 chronic aplastic anemia(CAA), 5 severe aplastic anemia(SAA) patients and 5 healthy subjects were detected with immunoflurescence assay, enriched by Panning way, and then cultured in vitro for CFU-GM, BFU-E and CFU-E in the presence of recombinant HGFs.


RESULTS
CD34 positive rates of the bone marrow mononuclear cells(MNC) of CAA, SAA and control groups were (1.05 +/- 0.51)%, (0.70 +/- 0.37)% and (1.27 +/- 0.45)%, respectively, and there was no difference among them (P > 0.05). After being enriched, CD34 positive cells in MNC of CAA, SAA and control groups increased to a similar level(P > 0.05). In the presence of G-CSF or GM-CSF, the enriched CD34 positive MNC of CAA and SAA patients formed similar CFU-GM number to those of normal controls. The numbers of BFU-E and CFU-E formed from the enriched CD34 positive MNC of CAA and SAA patients were also the same as that of normal control groups under the stimulation of erythropoietin and interleukin-3. Stem cell factor could cooperate with G-CSF, GM-CSF, and erythropoietin significantly increasing the numbers of CFU-GM, BFU-E and CFU-E of CAA, SAA and normal control's enriched CD34 positive MNC.


CONCLUSIONS
The bone marrow CD34 positive cells from aplastic anemia patients appears to be normal in percentages and in in vitro proliferation/differentiation capacities.
Chen
G
G
Institute of Hematology and Blood Diseases Hospital, CAMS, PUMC, Tianjin 300020.
Shao
Z
Z
Jia
H
H
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000473:pathology*; D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D002648:Child; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged
D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor