title: Enzyme activities of Borrelia burgdorferi sensu lato.
abstract: Activities of 19 enzymes were tested by the API ZYM system in 13 strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. lusitaniae, B. valaisiana) grown in liquid BSK-H medium supplemented with rabbit serum. All strains produced acid phosphatase, esterase (C4), esterase-lipase (C8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase. Nine strains also produced alkaline phosphatase, and three strains produced alpha-glucosidase. The API ZYM system probably cannot be used for differentiation between B. burgdorferi sensu lato genomospecies.
authors.affiliation: Department of Microbiology, Faculty of Science, Masaryk University, 602 00 Brno, Czechia.
authors.forename: M
authors.initials: M
authors.lastname: Heroldová
authors.forename: M
authors.initials: M
authors.lastname: Nĕmec
authors.forename: Z
authors.initials: Z
authors.lastname: Hubálek
authors.forename: J
authors.initials: J
authors.lastname: Halouzka
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D015748:Borrelia burgdorferi Group / Q000201:enzymology*; D004950:Esterases / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D008193:Lyme Disease / Q000382:microbiology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004950:Esterases; D008049:Lipase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D000520:alpha-Glucosidases; D007931:Leucyl Aminopeptidase