Isolation and characterization of two genes encoding ubiquitin fused to a ribosomal protein of 53 amino acids in rice.
We isolated and determined the nucleotide sequences of two genes encoding ubiquitin fused to a ribosomal protein, Ub-CEP52, from rice (Oryza sativa L.). The deduced amino-acid sequences of the two genes were found to be completely identical. The N-terminal region of 76 residues corresponds to ubiquitin, and the C-terminal region of 53 residues corresponds to ribosomal protein L40. A putative TATA-like sequence, a polypyrimidine sequence, and a similar sequence to telo-box were found in the promoter regions of the two genes. Furthermore, the putative tRNA(Pro) gene was found in the 5'-upstream region of one of them.
Kato
A
A
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan. atsushi@sci.hokudai.ac.jp
Nishi
R
R
Ozaki
M
M
D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics; D012361:RNA, Transfer, Pro / Q000737:chemistry / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D025801:Ubiquitin / Q000235:genetics*; D014452:Ubiquitins / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011498:Protein Precursors; D018749:RNA, Plant; D012361:RNA, Transfer, Pro; D012269:Ribosomal Proteins; D025801:Ubiquitin; D014452:Ubiquitins; C096228:ribosomal protein L40, rat; C045677:ubiquitin precursor