Determination of astragaloside IV in Radix astragali (Astragalus membranaceus var. monghulicus) using high-performance liquid chromatography with evaporative light-scattering detection.
A reverse-phase high-performance liquid chromatographic method is developed for the determination of astragaloside IV, a characteristic constituent in Radix Astragali. Samples are analyzed by means of a reverse-phase column (Zorbax Eclipse XDB C18) using acetonitrile and water under gradient conditions as the mobile phase for 30 min. An evaporative light-scattering detector is used and set at an evaporating temperature of 43 degrees C with a nebulizing gas (compressed air) pressure of 3.4 bar. The detection limit (signal-to-noise ratio > 5) of astragaloside IV is 40 ng on-column.
Li
W
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Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 60612, USA.
Fitzloff
J F
JF
D027883:Astragalus Plant / Q000737:chemistry*; D027885:Astragalus propinquus; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000032:analysis*; D008027:Light; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012542:Scattering, Radiation; D014315:Triterpenes
D005960:Glucosides; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A