title: Catalysis of NO production by a molybdoenzyme model.
abstract: [reaction--see text] Nitric oxide (NO) is an important biological messenger molecule. Nitrates, including nitroglycerin (GTN), are clinically important vasodilators believed to be biotransformed in vivo to NO, a 3e(-) reduction. Molybdenum hydrotris-(3,5-dimethyl-1-pyrazolyl) borate complex (MoTPB) was shown to be an efficient catalyst of GTN degradation, with triphenylphosphine (Ph(3)P) acting as reducing cofactor, producing significant amounts of NO. MoTPB/Ph(3)P is an excellent enzyme model system, showing the feasibility of nitrate biotransformation mediated by a molybdoenzyme.
authors.affiliation: Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Murray
authors.forename: D
authors.initials: D
authors.lastname: Macartney
authors.forename: G R
authors.initials: GR
authors.lastname: Thatcher
mesh_terms: D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D008163:Luminescent Measurements; D008954:Models, Biological; D008982:Molybdenum; D009569:Nitric Oxide / Q000138:chemical synthesis* / Q000378:metabolism; D005996:Nitroglycerin / Q000737:chemistry; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D010084:Oxidation-Reduction; D010088:Oxidoreductases; D010100:Oxygen / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; C442339:molybdenum hydrotris-(3,5-dimethyl-1-pyrazolyl)borate; C061896:triphenylphosphine; D009569:Nitric Oxide; D008982:Molybdenum; D010088:Oxidoreductases; D005996:Nitroglycerin; D010100:Oxygen