Synthesis of sulfated colominic acids and their interaction with fibroblast growth factors.
Colominic acid, an alpha (2-->8)-linked poly(sialic acid), was sulfated and characterized by NMR spectroscopy. During sulfation, the secondary hydroxyl group at C-4 had almost the same reactivity as the primary hydroxyl group at C-9, while the secondary hydroxyl group at C-7 was hardly substituted. Analysis by molecular modeling suggested that the lack of substitution at C-7 was due to a steric hindrance. A mobility shift assay indicated that FGF-2 bound to the sulfated colominic acid. Synthetic sulfated colominic acid potentiated the mitogenic activity of FGFs for fibroblasts in the same manner as heparin. Sulfated colominic acid with a low degree of sulfation was able to potentiate FGF activity. Regardless of the degree of sulfation, sulfated colominic acid-induced cytotoxicity was not observed. It was suggested that the carboxyl groups in sulfated colominic acid cooperate with the sulfate groups to reinforce the interaction with FGFs and to reduce the cytotoxicity of sulfated colominic acids.
Kunou
M
M
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4295 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Koizumi
M
M
Shimizu
K
K
Kawase
M
M
Hatanaka
K
K
D016475:3T3 Cells; D000818:Animals; D002942:Circular Dichroism; D005346:Fibroblast Growth Factors / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008934:Mitogens / Q000138:chemical synthesis / Q000494:pharmacology; D008958:Models, Molecular; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000737:chemistry
D008934:Mitogens; D011134:Polysaccharides; D013431:Sulfates; D005346:Fibroblast Growth Factors; C014420:colominic acid