[The tissue culture of medicinal plant Trichosanthes kirilowii and its protein analysis].
We reported preliminary results of rapid propagation, callus induction and regeneration of Trichosanthes Kirilowii, and its protein analysis. Pre-existing meristerms regenerate shoots very rapidly when grown on MS medium containing 0.5 or 1.0 mg/L 6-BA; calli could be induced from leaf sections when put on MS medium containing 4.0 mg/L 6-BA + 0.2 mg/L IAA; shoots regenerated successfully 30 days after calli induction and the differentiation ratio was one shoot out of every four leaf sections; and all shoots gave rise to roots after removing onto MS medium containing 0.1 mg/L NAA and 100% survived when transplanted into soil. Very excitingly, these plants produced small tubers in one month, where satisfactory expression of TCS protein was detected by Western blot analysis.
Zheng
S S
SS
Oil Crops Research Institute of Yunnan Academy of Agriculture Science, Kunming, 650205, China.
Yuan
H Y
HY
Wang
L J
LJ
An
C C
CC
Chen
Z L
ZL
D003470:Culture Media; D046508:Culture Techniques; D010940:Plant Proteins / Q000032:analysis*; D012038:Regeneration; D029778:Trichosanthes / Q000737:chemistry / Q000254:growth & development*
D003470:Culture Media; D010940:Plant Proteins