title: Molecular characterisation of mouse and human TSSC6: evidence that TSSC6 is a genuine member of the tetraspanin superfamily and is expressed specifically in haematopoietic organs.
abstract: Previous analyses of the murine and human TSSC6 (also known as Phemx) proteins were not carried out using the full length sequence. Using 5'-RACE and cDNA library screening, we identified an additional 5' sequence for both the murine Tssc6 cDNA and its human homologue TSSC6. This novel sequence encodes a 5' exon encoding an in frame, upstream start codon, an N-terminal cytoplasmic domain and a transmembrane domain. The deduced, and now full length, murine and human TSSC6 proteins contained four hydrophobic regions together with other features characteristic of the tetraspanin superfamily. Computational analyses of the full length sequences show that TSSC6 is a genuine, albeit relatively divergent member of this superfamily. Using RNA from a number of mouse tissues, we identified seven splice variants of Tssc6. Splice variants of the human gene were also detected. Tssc6 expression was detected early in embryogenesis in primitive blood cells and was confined to haematopoietic organs in the adult mouse. Tssc6 expression was detected in many haematopoietic cell lines and was highest in cell lines of the erythroid lineage.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Vic, Autralia. robb@wehi.edu.au
authors.forename: L
authors.initials: L
authors.lastname: Robb
authors.forename: J
authors.initials: J
authors.lastname: Tarrant
authors.forename: J
authors.initials: J
authors.lastname: Groom
authors.forename: M
authors.initials: M
authors.lastname: Ibrahim
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Borobakas
authors.forename: M D
authors.initials: MD
authors.lastname: Wright
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D005091:Exons; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D059470:Tetraspanins
chemical_list: D003062:Codon; D008565:Membrane Proteins; C417909:Tspan32 protein, mouse; D011506:Proteins; C485950:TSPAN32 protein, human; D059470:Tetraspanins