title: Crystal structure of an Xrcc4-DNA ligase IV complex.
abstract: A complex of two proteins, Xrcc4 and DNA ligase IV, plays a fundamental role in DNA non-homologous end joining (NHEJ), a cellular function required for double-strand break repair and V(D)J recombination. Here we report the crystal structure of human Xrcc4 bound to a polypeptide that corresponds to the DNA ligase IV sequence linking its two BRCA1 C-terminal (BRCT) domains. In the complex, a single ligase chain binds asymmetrically to an Xrcc4 dimer. The helical tails of Xrcc4 undergo a substantial conformational change relative to the uncomplexed protein, forming a coiled coil that unwinds upon ligase binding, leading to a flat interaction surface. A buried network of charged hydrogen bonds surrounded by extensive hydrophobic contacts explains the observed tightness of the interaction. The strong conservation of residues at the interface between the two proteins provides evidence that the observed mode of interaction has been maintained in NHEJ throughout evolution.
authors.affiliation: Department of Biochemistry, Cambridge University, Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: B L
authors.initials: BL
authors.lastname: Sibanda
authors.forename: S E
authors.initials: SE
authors.lastname: Critchlow
authors.forename: J
authors.initials: J
authors.lastname: Begun
authors.forename: X Y
authors.initials: XY
authors.lastname: Pei
authors.forename: S P
authors.initials: SP
authors.lastname: Jackson
authors.forename: T L
authors.initials: TL
authors.lastname: Blundell
authors.forename: L
authors.initials: L
authors.lastname: Pellegrini
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D019281:Dimerization; D006801:Humans; D006860:Hydrogen Bonding; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D055672:Static Electricity
chemical_list: D004268:DNA-Binding Proteins; C000607893:LIG4 protein, human; D046911:Macromolecular Substances; C101467:XRCC4 protein, human; D011088:DNA Ligases; D000072481:DNA Ligase ATP