title: A comparison of the use of an ATP-based bioluminescent assay and image analysis for the assessment of bacterial adhesion to standard HEMA and biomimetic soft contact lenses.
abstract: The aim of this study was to investigate in vitro adhesion of clinically relevant bacteria to standard HEMA and novel biomimetic soft contact lenses (SCL) using bioluminescent ATP assay and image analysis. Unworn SCL were incubated with Pseudomonas aeruginosa, Staphylococcus epidermidis or Serratia marcescens suspended in sterile phosphate buffered saline (PBS). The level of bacterial adhesion after 1, 2, 4, 6 and 18h, was assessed using both image analysis and a bioluminescent ATP assay. Species differences in the overall level of adhesion to the different types of lens were observed using both measurement techniques. Generally bacterial adhesion was shown to peak at 4-6 h, then decline to a much lower level by 18 h. After 4 h, adhesion of all species of bacteria to the biomimetic SCL (omafilcon A) was found to be significantly lower than to the standard HEMA SCL (polymacon) (p<0.05. Student's t-test, n = 4). Both these techniques demonstrated that novel biomimetic SCL materials exhibit significantly lower bacterial adhesion in vitro compared to standard HEMA SCL materials. SCL manufactured with these novel biomimetic materials may reduce the risk of infection.
authors.affiliation: Biomedical Materials Research Group, School of Pharmacy and Biomolecular Sciences, University of Brighton Moulsecoomb, UK.
authors.forename: C S
authors.initials: CS
authors.lastname: Andrews
authors.forename: S P
authors.initials: SP
authors.lastname: Denyer
authors.forename: B
authors.initials: B
authors.lastname: Hall
authors.forename: G W
authors.initials: GW
authors.lastname: Hanlon
authors.forename: A W
authors.initials: AW
authors.lastname: Lloyd
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000009:adverse effects*; D018441:Biofilms / Q000254:growth & development; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D007634:Keratitis / Q000209:etiology / Q000517:prevention & control; D008163:Luminescent Measurements; D008422:Materials Testing; D008689:Methacrylates; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity / Q000502:physiology; D012706:Serratia marcescens / Q000472:pathogenicity / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000472:pathogenicity / Q000502:physiology; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; D000255:Adenosine Triphosphate