Gene frequencies of human neutrophil antigens in the Tunisian blood donors and Berbers.
Human neutrophil antigens play an important role in provoking immune neutropenia and transfusion-reactions. The aim of this study was to determine granulocyte-specific antigens on the neutrophil Fc gamma receptor IIIb (Fc gamma RIIIb, CD16b), namely, the HNA-1a(NA1) and HNA-1b(NA2) antigens and their gene frequencies in Tunisian blood donors and Berbers. One hundred and ninety-nine unrelated healthy Tunisian blood donors and Berbers were typed for HNA-1a and HNA-1b(NA1 and NA2), using polymerase chain reaction with sequence-specific primers (PCR-SSP). In 24 granulocyte samples, the HNA-1a and HNA-1b phenotypes was additionally determined by the granulocyte immunofluorescence test (GIFT) and correlated with the genotyping results. A subsequent analysis of the genotyping study showed that, the HNA-1a and HNA-1b gene frequencies observed, were 0.342 and 0.658 for Berbers, and 0.311 and 0.668 for blood donors, respectively. In the genotyping study conducted, it was determined that the HNA-1a and HNA-1b gene frequencies observed in Tunisian blood donors and Berbers are similar to those previously reported in other white populations.
Abid
S
S
National Blood Transfusion Center, Tunis, Tunisia.
Zili
M
M
Bouzid
L
L
Kibech
R
R
Foudhaili
N
N
Joudi
K
K
Ren Regaya
Z
Z
Abdennaji
B
B
Mrad
R
R
Boukef
K
K
D001782:Blood Donors; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D009504:Neutrophils / Q000276:immunology*; D014416:Tunisia
D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human; C072753:neutrophil-specific antigen NA2