title: Relationship of the microvascular type to the tumor size, arterialization and dedifferentiation of human hepatocellular carcinoma.
abstract: Unlike normal liver with the sinusoids, hepatocellular carcinomas (HCCs) possess capillaries. Whether these capillaries derive from the sinusoids remains unclear in human HCCs. This study aimed to examine sinusoidal capillarization in human HCCs and its relationship to the tumor size, arterialization and dedifferentiation. Thirty-eight HCCs with a diameter of 10 - 140 mm were pathologically and angiographically examined. By electron microscopy, the microvasculature of tumors was classified into sinusoidal, intermediate and capillary types, which were all negative, partially positive and all positive, respectively, for four parameters, i.e., endothelial defenestration, continuous basement membrane, lack of Kupffer cells, and lack of lipid-containing hepatic stellate cells. Well-, moderately and poorly differentiated HCCs displayed sinusoidal / intermediate / capillary types, intermediate / capillary types and only capillary type, respectively, suggesting the transition from the sinusoids to capillaries in well-differentiated (and probably moderately differentiated) HCCs. Furthermore, well-differentiated HCCs with a diameter of less than 30 mm often received preferential portal venous blood, while moderately and poorly differentiated ones were all supplied with arterial blood, indicating a relationship between dedifferentiation and arterialization. In contrast, the microvascular type displayed no significant relationship with tumor size or arterialization in well-differentiated HCCs. The present study has demonstrated that sinusoidal capillarization occurs in human well-differentiated HCCs and seems to be related to dedifferentiation of parenchymal tumor cells, but not to tumor size or arterialization.
authors.affiliation: Hepato-Biliary-Pancreatic and Gastrointestinal Surgery, Osaka City University Graduated School of Medicine, Abeno-ku, Osaka 545-8585. takatsugu@msic.med.osaka-cu.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Hirohashi
authors.forename: K
authors.initials: K
authors.lastname: Kaneda
authors.forename: T
authors.initials: T
authors.lastname: Ikebe
authors.forename: S
authors.initials: S
authors.lastname: Mikami
authors.forename: T
authors.initials: T
authors.lastname: Uenishi
authors.forename: A
authors.initials: A
authors.lastname: Kanazawa
authors.forename: S
authors.initials: S
authors.lastname: Takemura
authors.forename: T
authors.initials: T
authors.lastname: Shuto
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Kubo
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.forename: H
authors.initials: H
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D000368:Aged; D000792:Angiography; D006528:Carcinoma, Hepatocellular / Q000098:blood supply* / Q000473:pathology* / Q000648:ultrastructure; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged