The interactions of cartilage proteoglycans with collagens are determined by their structures.
In the present work, the interaction of aggrecan, decorin and biglycan isolated from pig laryngeal cartilage and of the three squid cartilage proteoglycans with collagen type I and II was studied. The interaction was examined under conditions allowing the formation of collagen fibrils. It was found that biglycan interacted strongly with collagen type II and not with type I and the interaction seemed to proceed exclusively through its core proteins. Decorin interacted with collagen type I but not with type II. Aggrecan interacted very poorly with both collagen types. The two squid proteoglycans of large size, D1D1A and D1D2, interacted only with collagen type I through both glycosaminoglycans and core proteins. The third squid proteoglycan of small size, D1D1B, interacted poorly only with collagen type I. The results suggested that the interactions of cartilage proteoglycans with collagen were mainly due to the primary structure of both molecules, and would contribute to the maintenance of the integrity of the tissue. The biochemical significance of these interactions might be more critical in aged vertebrate cartilage, where loss of aggrecan and increase of the small proteoglycans was observed, a large proportion of which is found in the extracellular matrix free of glycosaminoglycan chains.
Vynios
D H
DH
Laboratory of Biochemistry, Department of Chemistry, University of Patras, 261 10 Patras, Greece. vynios@chemistry.upatras.gr
Papageorgakopoulou
N
N
Sazakli
H
H
Tsiganos
C P
CP
D000125:Achilles Tendon / Q000737:chemistry; D053674:Aggrecans; D000595:Amino Acid Sequence; D000818:Animals; D058578:Biglycan; D002417:Cattle; D024042:Collagen Type I / Q000494:pharmacology; D024043:Collagen Type II / Q000494:pharmacology; D017364:Collagenases / Q000378:metabolism; D049832:Decapodiformes; D058575:Decorin; D016326:Extracellular Matrix Proteins; D066298:In Vitro Techniques; D007700:Kinetics; D007817:Laryngeal Cartilages / Q000737:chemistry*; D037181:Lectins, C-Type; D011485:Protein Binding; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013552:Swine; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors
D053674:Aggrecans; D058578:Biglycan; D024042:Collagen Type I; D024043:Collagen Type II; D058575:Decorin; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; D017364:Collagenases