title: Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat.
abstract: The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10(-7), 10(-8) or 10(-9) CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10(-9) CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.
authors.affiliation: Centre for Diagnostics and Research in Avian Pathology, Federal University of Rio Grande do Sul, RS, Brazil. luruschel@bol.com.br
authors.forename: L R
authors.initials: LR
authors.lastname: dos Santos
authors.forename: V P
authors.initials: VP
authors.lastname: do Nascimento
authors.forename: S D
authors.initials: SD
authors.lastname: de Oliveira
authors.forename: M L
authors.initials: ML
authors.lastname: Flores
authors.forename: A P
authors.initials: AP
authors.lastname: Pontes
authors.forename: A R
authors.initials: AR
authors.lastname: Ribeiro
authors.forename: C T
authors.initials: CT
authors.lastname: Salle
authors.forename: R F
authors.initials: RF
authors.lastname: Lopes
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011202:Poultry Products / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial