title: Cellular solid-phase binding assay and mass spectrometry for screening of alpha 4 beta 7 integrin antagonists.
abstract: A qualitative cellular solid-phase binding assay for screening alpha 4 beta 7 integrin antagonists attached via photolinker to TentaGel Macrobeads has been developed. An activation of the integrins with Mn(2+) was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.
authors.affiliation: Institut für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Gottschling
authors.forename: J
authors.initials: J
authors.lastname: Boer
authors.forename: A
authors.initials: A
authors.lastname: Schuster
authors.forename: B
authors.initials: B
authors.lastname: Holzmann
authors.forename: H
authors.initials: H
authors.lastname: Kessler
mesh_terms: D002448:Cell Adhesion; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D016023:Integrins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D016023:Integrins; C071777:integrin alpha4beta7