Consequences of gene targeting procedures for behavioural responses and morphological development of newborn mice.
In this study the effects of gene targeting procedures on the early behaviour and morphological development of the resulting offspring have been investigated. Six groups of mice, each having undergone a specific aspect of the biotechnological procedure, (including electroporation, microinjection and/or embryo culture) and one control group, were compared. Development of behaviour, morphological characteristics and body weight of the progeny were tested daily from birth to weaning (0-3 weeks) for all groups. No significant differences in behaviour or morphological development were observed. However, the occurrence of increased (perinatal) pup mortality and increased body weight in the procedural groups, indicates that during the production of gene targeted mice, some of the normal physiological and/or developmental processes can be affected. Therefore, gene targeting procedures should always be accompanied by careful monitoring of health and welfare of the resulting offspring.
van der Meer
M
M
Department of Laboratory Animal Science, Utrecht University, The Netherlands. m.vandermeer@las.vet.uu.nl
Baumans
V
V
Hofhuis
F M
FM
Olivier
B
B
van Zutphen
B F
BF
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001522:Behavior, Animal / Q000502:physiology*; D001724:Birth Weight; D001835:Body Weight; D016022:Case-Control Studies; D005260:Female; D005818:Genetic Engineering / Q000379:methods; D008098:Litter Size; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic / Q000502:physiology*; D011247:Pregnancy