title: Characterization of the adhesin of Escherichia coli F18 fimbriae.
abstract: Previous research has suggested that the adhesin encoded by the F18 fimbrial operon in Escherichia coli is either the FedE or FedF protein. In this work, we show that anti-FedF antibodies, unlike anti-FedE serum, were able to inhibit E. coli adhesion to porcine enterocytes. Moreover, specific adhesion to enterocytes was shown with purified FedF-maltose binding protein.
authors.affiliation: Faculty of Veterinary Medicine, Department of Basic Veterinary Sciences, Section of Microbiology, 00014 University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Smeds
authors.forename: K
authors.initials: K
authors.lastname: Hemmann
authors.forename: M
authors.initials: M
authors.lastname: Jakava-Viljanen
authors.forename: S
authors.initials: S
authors.lastname: Pelkonen
authors.forename: H
authors.initials: H
authors.lastname: Imberechts
authors.forename: A
authors.initials: A
authors.lastname: Palva
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D000907:Antibodies, Bacterial; D001422:Bacterial Adhesion; D003001:Cloning, Molecular; D020895:Enterocytes / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000276:immunology / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000235:genetics; D019084:Fluorescent Antibody Technique, Indirect; D007082:Ileum / Q000166:cytology / Q000382:microbiology; D007583:Jejunum / Q000166:cytology / Q000382:microbiology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins; D013552:Swine
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; C440653:FedF protein, E coli; D011993:Recombinant Fusion Proteins