First images of a glutamate receptor ion channel: oligomeric state and molecular dimensions of GluRB homomers.
We have expressed, purified, and characterized glutamate receptor ion channels (GluR) assembled as homomers of the subunit GluRB. For the first time, single-milligram quantities of biochemically homogeneous GluR have been obtained. The protein exhibits the expected pharmacological profile and a high specific activity for ligand binding. Density-gradient centrifugation reveals a uniform oligomeric assembly and a molecular mass suggesting that the channel is a tetramer. On the basis of electron microscopic images, the receptor appears to form an elongated structure that is visualized in several orientations. The molecular dimensions of the molecule are approximately 11 x 14 x 17 nm, and solvent-accessible features can be seen; these may contribute to formation of the ion-conducting pathway of the channel. The channel dimensions are consistent with an overall 2-fold symmetric assembly, suggesting that the tetrameric receptor may be a dimer of dimers.
Safferling
M
M
Ion Channel Structure Research Group, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Tichelaar
W
W
Kümmerle
G
G
Jouppila
A
A
Kuusinen
A
A
Keinänen
K
K
Madden
D R
DR
D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D007473:Ion Channels / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D007700:Kinetics; D008024:Ligands; D008854:Microscopy, Electron; D051381:Rats; D018091:Receptors, AMPA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000648:ultrastructure; D018411:Spodoptera / Q000235:genetics
D007473:Ion Channels; D008024:Ligands; D018091:Receptors, AMPA; D011994:Recombinant Proteins; C115953:glutamate receptor type B