Enteral exposure to crude red kidney bean lectin induces maturation of the gut in suckling pigs.
The present investigation characterized the effect of red kidney bean lectin exposure on gut maturation and function in young piglets. Eleven suckling pigs were given by stomach tube a crude red kidney bean lectin preparation (containing about 25% lectin, 400 mg/kg BW) (lectin-treated pigs) at 10, 11, and 12 d of life, and an additional 16 pigs (control pigs) were given saline instead. On the next day, the intestinal absorptive capacity was determined in vivo, and on the 14th d of life the piglets were killed and organs and small intestine samples were collected for analyses and in vitro permeability experiments. The lectin-treated pigs showed an increase in stomach weights and mucosa thickness, whereas no weight effect was found for the small intestine, spleen, liver, or adrenals. Morphometric analyses of the small intestine in lectin-treated pigs showed a decrease in villus heights, an increase in crypt depths and crypt cell mitotic indices, and fewer vacuolated enterocytes per villus and reduced vacuole size. Lectin treatment also resulted in a decrease in the absorption of different-sized marker molecules after gavage feeding, a decrease in intestinal marker permeability, and a change in small intestinal disaccharidase activities, with increased maltase and sucrase activities. The size of the pancreatic acini was also greater in the lectin-treated pigs, but no increases in enzyme content or pancreatic weight could be determined. In addition, the blood plasma levels of cholecystokinin were higher in the lectin-treated than in the control pigs. The results indicate that exposure to crude red kidney bean lectin induces structural and functional maturation of the gut and pancreatic growth in young suckling piglets. This possibility of inducing gut maturation may lead to an improvement in the piglets' ability to adapt to weaning and to an increase in the growth and health of these animals.
Rådberg
K
K
Department of Animal Physiology, University of Lund, Helgonavägen, Sweden. Bjorn.Westrom@zoofys.lu.se
Biernat
M
M
Linderoth
A
A
Zabielski
R
R
Pierzynowski
S G
SG
Weström
B R
BR
D000818:Animals; D000833:Animals, Suckling / Q000254:growth & development*; D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D002766:Cholecystokinin / Q000097:blood; D004064:Digestive System / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D004186:Disaccharidases / Q000378:metabolism; D004750:Enteral Nutrition; D007408:Intestinal Absorption / Q000187:drug effects; D008871:Microvilli; D008940:Mitotic Index; D009929:Organ Size; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D010539:Permeability; D010835:Phytohemagglutinins / Q000008:administration & dosage* / Q000494:pharmacology; D011897:Random Allocation; D013552:Swine / Q000254:growth & development*; D014886:Weaning
D015415:Biomarkers; D010835:Phytohemagglutinins; D002766:Cholecystokinin; D004186:Disaccharidases