title: Deglycosylated mucin as a substrate in enzymatic O-glycosylation in vitro.
abstract: To obtain the apomucin as a substrate for glycosyltransferases activity testing, the pig gastric mucin deglycosylation by chemical method was carried out. Resulted apomucin, rich in Ser, Thr and Pro residues, was as good carbohydrate acceptor in enzymatic O-glycosylation in vitro, as synthetic peptide, analogue of MUC2 mucin tandem repeats sequence.
authors.affiliation: Department of General and Organic Chemistry, Medical Academy of Białystok.
authors.forename: A
authors.initials: A
authors.lastname: Paszkiewicz-Gadek
authors.forename: H
authors.initials: H
authors.lastname: Porowska
authors.forename: D
authors.initials: D
authors.lastname: Kisiel
authors.forename: A
authors.initials: A
authors.lastname: Gindzieński
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004187:Disaccharides / Q000138:chemical synthesis; D005752:Gastric Mucins / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D055262:Mucin-2; D009077:Mucins / Q000235:genetics; D013379:Substrate Specificity; D013552:Swine
chemical_list: D004187:Disaccharides; D005752:Gastric Mucins; D055262:Mucin-2; D009077:Mucins; C053366:apomucin; D016695:Glycosyltransferases