title: Technical optimization of RhD zygosity determination by real-time quantitative polymerase chain reaction: implication for fetal RhD status determination by maternal plasma.
abstract: We have recently reported the development of a multiplex, real-time quantitative polymerase chain reaction (PCR) assay for RhD zygosity determination based on the coamplification and quantification of RHD-specific sequences in relation to a reference gene, albumin. This paper discusses the optimization procedure and technical parameters of this multiplex assay.
authors.affiliation: Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories.
authors.forename: R W
authors.initials: RW
authors.lastname: Chiu
authors.forename: M F
authors.initials: MF
authors.lastname: Murphy
authors.forename: C
authors.initials: C
authors.lastname: Fidler
authors.forename: J S
authors.initials: JS
authors.lastname: Wainscoat
authors.forename: Y M
authors.initials: YM
authors.lastname: Lo
mesh_terms: D000418:Albumins / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
chemical_list: D000418:Albumins; D012204:Rh-Hr Blood-Group System