Fetal DNA diagnosis from maternal blood: PEP-TaqMan PCR analysis of a single nucleated erythrocyte (NRBC).
Development of a real-time quantitative TaqMan PCR assay permits the high-quality analysis of DNA or RNA. This study demonstrates the successful detection of single locus genes from single NRBCs, retrieved by micromanipulation of cells on a blood smear slide. Quantitative data in relation to the concentration of a single locus DNA amplified by PEP from a single NRBC are also provided. In summary, a PEP-TaqMan system can potentially be a powerful tool for noninvasive fetal DNA diagnosis through the use of maternal blood.
Ikawa
K
K
Ishikawa Health Service Association, Kanazawa, Japan. kazumi-i@po.sphere.ne.jp
Yamafuji
K
K
Ukita
T
T
Kuwabara
S
S
Igarashi
T
T
Takabayashi
H
H
D002467:Cell Nucleus / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D005315:Fetal Diseases / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D004247:DNA