[Study of apoptosis induced by indomethacin in chronic myeloid leukemia].
OBJECTIVE
To explore the effect of indomethacin(IN) on the apoptosis of chronic myeloid leukemia(CML) cells, and its molecular mechanisms, for the purpose of screening a new antileukemic agent.


METHODS
CML cell line K562 and fresh bone marrow cells from 6 untreated Ph+ CML patients were used for in vitro culture study. The effects of IN on cells were determined by cell morphology, flow cytometry, DNA electrophoresis, and RT-PCR.


RESULTS
1. IN induced apoptosis of K562 and fresh CML cells and inhibited the proliferation of K562 cells. 2. A synergic effect of inducing K562 cell apoptosis was observed when IN combined with Vp16. 3. IN down-regulated the level of bcl-2 mRNA without changing the level of bax mRNA in K562 cells.


CONCLUSIONS
IN can induce apoptosis and inhibit proliferation in CML cells, and increase the sensitivity of CML cells to Vp16. Down-regulation of bcl-2 mRNA may be one of the mechanisms of CML cell apoptosis induced by IN.
Tu
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C
Laboratory of Molecular Hematology, Division of Hematology, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Zhang
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G
Lu
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H
D017209:Apoptosis / Q000187:drug effects*; D015536:Down-Regulation; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D020014:K562 Cells / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D007213:Indomethacin