[Expression patterns of platelet membrane glycoprotein in healthy adults].
OBJECTIVE
To study the expression patterns of 6 kinds of platelet membrane glycoprotein(GP) in resting and activated platelets of healthy adults.


METHODS
The molecule numbers of platelet GPIIIa, IIb/IIIa, Ib, IX, V and P-selectin on resting platelets or platelets activated by thrombin receptor activating peptide (TRAP) in 60 healthy adults (male/female = 1/1) were detected by flow cytometry.


RESULTS
The expression of GPIIb/IIIa and GPIIIa seemed to decrease with the increase of age in the resting and activated platelets of males. The coefficients of correlation(r) in resting platelets were -0.409 and -0.534 (P < 0.05), respectively; whereas this phenomenon were not found in females. The expression of GPIb was reduced while the expressions of GPIIb/IIIa, IIIa, P-selectin increased after the platelets were activated by TRAP. There was a negative relationship between resting and activated platelets in the expression of P-selectin. The molecule number of GPIb on the surface of resting platelet was about 30,000, the ratio of GPIb to GPV was 3:1.


CONCLUSIONS
It seems that there exists some difference with age and sex in the expression of platelet membrane glycoproteins in healthy persons.
Huang
F
F
Tianjin First Central Hospital, Tianjin Institute of Thrombosis and Hemostasis, Tianjin 300192.
Liu
R
R
Liu
L
L
D000328:Adult; D000367:Age Factors; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000378:metabolism*; D015539:Platelet Activation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D012737:Sex Factors
D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins