The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture.
We report here the isolation of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (AtSERK1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. The AtSERK1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. The AtSERK1 gene is first expressed in planta during megasporogenesis in the nucellus [corrected] of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fertilization. After fertilization, AtSERK1 expression is seen in all cells of the developing embryo until the heart stage. After this stage, AtSERK1 expression is no longer detectable in the embryo or in any part of the developing seed. Low expression is detected in adult vascular tissue. Ectopic expression of the full-length AtSERK1 cDNA under the control of the cauliflower mosaic virus 35S promoter did not result in any altered plant phenotype. However, seedlings that overexpressed the AtSERK1 mRNA exhibited a 3- to 4-fold increase in efficiency for initiation of somatic embryogenesis. Thus, an increased AtSERK1 level is sufficient to confer embryogenic competence in culture.
Hecht
V
V
Laboratory of Molecular Biology, Wageningen University, 6703HA Wageningen, The Netherlands.
Vielle-Calzada
J P
JP
Hartog
M V
MV
Schmidt
E D
ED
Boutilier
K
K
Grossniklaus
U
U
de Vries
S C
SC
D017360:Arabidopsis / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D017796:Caulimovirus; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005306:Fertilization; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005810:Multigene Family; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015053:Zygote / Q000254:growth & development
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011494:Protein Kinases; C440035:SERK1 protein, Arabidopsis