Stimulation of the regrowth of MPP+-damaged dopaminergic fibers by the treatment of mesencephalic cultures with basigin.
Basigin (Bsg) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and widely expressed in the central nervous system. To elucidate functional role of Bsg in the central nervous system, the effects of its glutathione-S-transferase (GST) fusion protein on the number and neurite outgrowth of cultured rat mesencephalic dopaminergic neurons were measured. The fusion protein was not able to promote the survival and neurite outgrowth of tyrosine hydroxylase (TH)-positive neurons under serum-free condition. However, the treatment of 1-methyl-4-phenylpyridinium (MPP+)-exposed cultures with the fusion protein resulted in stimulation of the regrowth of damaged TH-positive fibers. Basic fibroblast growth factor (bFGF) also stimulated the regrowth of neurites in damaged neurons. These results indicate that Bsg may play an important role in the regrowth of damaged dopaminergic fibers.
Mitsumoto
Y
Y
Neurodegenerative Diseases Research Group, Second Institute of New Drug Research, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan. y_mitsumoto@research.otsuka.co.jp
Watanabe
A
A
Miyauchi
T
T
Jimma
F
F
Moriizumi
T
T
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D051926:Basigin; D001798:Blood Proteins; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004298:Dopamine / Q000502:physiology; D004622:Embryo, Mammalian; D006540:Herbicides / Q000494:pharmacology; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D008636:Mesencephalon / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D009412:Nerve Fibers / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; C497886:BSG protein, human; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D006540:Herbicides; D008562:Membrane Glycoproteins; D051926:Basigin; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine