title: Enhancement of beta-sitosterol transformation in Mycobacterium vaccae with increased cell wall permeability.
abstract: Mycobacterium vaccae exposed to compounds which are known to disorganise the cell wall composition and architecture (protamine, glycine) showed increased specific activity in beta-sitosterol biotransformation to androstene derivatives, intennediates in the production of most medical steroids. GC/MS analysis of free lipid fatty acids revealed higher content of unsaturated compounds, mainly C16:1 and C18:1 in protamine- and glycine-treated cells than that in control cells, which seems to change the permeability features of the cell wall barrier, facilitating hydrophobic beta-sitosterol diffusion.
authors.affiliation: Centre for Microbiology and Virology, Polish Academy of Sciences, 93-232 Lódź, Lodowa 106, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Korycka-Machała
authors.forename: A
authors.initials: A
authors.lastname: Rumijowska-Galewicz
authors.forename: K
authors.initials: K
authors.lastname: Lisowska
authors.forename: A
authors.initials: A
authors.lastname: Ziolkowskit
authors.forename: L
authors.initials: L
authors.lastname: Sedlacze
mesh_terms: D000730:Androstadienes / Q000378:metabolism; D000735:Androstenedione / Q000096:biosynthesis; D001711:Biotransformation; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D009161:Mycobacterium / Q000187:drug effects / Q000378:metabolism*; D011479:Protamines / Q000494:pharmacology; D012855:Sitosterols / Q000378:metabolism*
chemical_list: D000730:Androstadienes; D005227:Fatty Acids; D011479:Protamines; D012855:Sitosterols; C006573:1,4-androstadiene-3,17-dione; D000735:Androstenedione; C025473:gamma-sitosterol; D005998:Glycine