[Preliminary study on the immune response in C57BL/6 mice vaccinated by nucleic acid encoding paramyosin of Chinese S. japonicum].
OBJECTIVE
To study the characteristics of immune response in C57 BL/6 mice by nucleic acid encoding full-length paramyosin of Chinese S. japonicum.


METHODS
Total RNA was isolated from adult Chinese S. japonicum using TRIzol reagent. The full-length cDNA encoding paramyosin of Chinese S. japonicum was amplified by RT-PCR and cloned into expression vector pcDNA/Amp (pCMV-SjC97), which was intramuscularly injected into C57 BL/6 mice. The mice were vaccinated with DNA the three-week interval for a total of three inoculations. The specific anti-SjC97 IgM, IgG and IgE antibodies and IgG subclasses were determined by ELISA test.


RESULTS
The pCMV-SjC97 vaccine induced specific anti-SjC97 IgM and IgG antibodies. The antibody response correlated well with the administered amount of pCMV-SjC97. The IgG subclasses, predominantly IgG2a and IgG2b, were observed in the mice vaccinated with pCMV-SjC97, but no IgG1 and IgG3 responses were seen.


CONCLUSIONS
The strong immune response, especially Th1 type, can be induced in the mice with pCMV-SjC97 vaccine.
Zhou
S
S
Department of Immunology, Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Liu
S
S
Song
G
G
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000737:chemistry / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control; D014335:Tropomyosin / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D014335:Tropomyosin; D019444:Vaccines, DNA