Application of FTIR microscopy for the characterization of malignancy: H-ras transfected murine fibroblasts as an example.
Recently, microscopic FTIR is widely used in the field of biology and medicine. FTIR can detect biomolecular changes in the cells and tissues responsible for various disorders. In this report, we characterize the H-ras transfected fibroblasts and its normal control using microscopic FTIR. The intensity of the normal fibroblasts was higher than that of H-ras transfected fibroblasts. Our studies showed significant differences occur in the concentration of vital metabolites upon transformation. The DNA and carbohydrates level decreased in the transformed cells compared to the controls. A linear correlation could be found between the levels of carbohydrates and phosphate, while the RNA/DNA ratio varied inversely with glucose/phosphate levels.
Ramesh
J
J
Department of Physics, Ben-Gurion University, 84105, Beer-Sheva, Israel.
Salman
A
A
Hammody
Z
Z
Cohen
B
B
Gopas
J
J
Grossman
N
N
Mordechai
S
S
D016475:3T3 Cells; D000818:Animals; D002461:Cell Line, Transformed; D005347:Fibroblasts / Q000378:metabolism*; D011905:Genes, ras / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000175:diagnosis*; D010710:Phosphates / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D014162:Transfection
D010710:Phosphates; D005947:Glucose