Interaction between gene p53 and oncogene mdm2 in human glandular lung cancer cell line GLC-82.
OBJECTIVE
To study the interaction between oncogene mdm2 and wp53 in human glandular lung cancer cell line GLC-82.


METHODS
By lipofectamine-mediated DNA transfection, wp53 and mdm2 were transfected separately or co-transfected into GLC-82 cells via retrovival vector pDOR-neo, a carrier of wp53 and mdm2.


RESULTS
The growth of GLC-82 cells was blocked and their DNA synthesis inhibited by wp53, its colony-forming rate in soft agar culture and the tumorigenicity in nude mice declined and mdm2 antagonized the function of wp53.


CONCLUSIONS
After the recombinant vector pDOR-mdm2 was transfected into GLC-82 cells containing wp53, mdm2 partially deprives wp53 of its function of inhibiting the growth of GLC-82 cells.
Li
P
P
Department of Molecular Biology, Xijing Hospital, 4th Military Medical University, Xi'an 710032, China.
Rao
G
G
Chen
Y
Y
Ding
Z
Z
Yu
W
W
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000235:genetics; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D051736:Proto-Oncogene Proteins c-mdm2; D014162:Transfection; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C497206:MDM2 protein, human; C497207:Mdm2 protein, mouse; D051736:Proto-Oncogene Proteins c-mdm2