Fibroblast growth factor-2 interacts with free ribosomal protein S19.
Exogenous FGF-2 added to cells is internalized and part of it translocates to the nucleus of the cells. To get a better understanding of the FGF-2-induced signaling pathway, we looked for proteins associated with FGF-2 in the cytoplasm of the target cells. We first used the GST-FGF-2 to isolate cytoplasmic proteins complexes containing FGF-2 from S100 extract (supernatant 100,000g). Among the retrieved proteins, we focused our studies on RPS19, a protein of the 40S small ribosomal subunit. We showed that FGF-2 interacts directly with RPS19 in vitro. Second, we coimmunoprecipitated RPS19 and FGF-2 from a S240 extract (240,000g supernatant) prepared from FGF-2-stimulated cells and devoid of 40S ribosomal subunit. The result of these experiments suggest that a pool of free RPS19 exists in cells and that FGF-2 interacts in vivo with free RPS19.
Soulet
F
F
Laboratoire de Biologie Vasculaire, IPBS/CNRS, 205 Rte de Narbonne, 31077 Toulouse Cedex, France.
Al Saati
T
T
Roga
S
S
Amalric
F
F
Bouche
G
G
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D019921:Biotinylation; D015152:Blotting, Northern; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D015398:Signal Transduction
D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C040343:ribosomal protein S19; D016222:Fibroblast Growth Factor 2; D005982:Glutathione Transferase