[Structure and function of mouse p16INK4a locus].
Inactivation of p16INK4a is highly related with tumors. We obtained a 14.5 kb mouse genomic DNA fragment through screening the 129/SvJ mouse genomic library. Bioinformatics study suggests that the fragment contain three exons, which encode a 17,941 dolton polypeptide consisted of 168aa. The polypeptide has 7 potential phosphorylation sites, which indicates that the function of p16INK4a itself may be under the control of phosphorylation. Many interspersed elements and simple repeats were found in the noncoding region. These elements and repeats may cause retroposition and recombination and thus be responsible for some of the p16INK4a locus deletion. We also compared our sequence of exon 1 alpha with those published elsewhere and found that polymorphism may exist.
Gong
Z M
ZM
Medical Genetics Department, Second Military Medical University, Shanghai 200433, China. gongzhenming@yahoo.com
Yang
H
H
Fu
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D019942:Genes, p16; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid