[Impact of intracellular Na+ concentration alteration on intracellular aerobic glycolysis in skeletal muscles in sepsis].
OBJECTIVE
To evaluate the impact of the changes of the intracellular Na+ concentration on the cellular lactate production of different skeletal muscles in septic rats as compared to that of the normal ones.


METHODS
Using septic model of rats, we established the in vitro muscle incubation system with sufficient oxygen supply as well as the NADH fluoremetric method for the detection of trace amount of lactate in the samples. By using the specific Na+ ionorphor--monensin, the distribution of transmembrane Na+ was under control and the lactate production of the muscle cells were measured.


RESULTS
The intracellular glycolysis was activated by increasing of intracellular Na+ probably because of the mechanism of Na+ pump activation. The sensitivity of muscles to monensin varied according to different muscle fiber composition. The mechanism of the aerobic glycolysis activation in sepsis was identical to the roles of monensin in the cells.


CONCLUSIONS
The increase of the permeability of Na+ in skeletal muscle cells as well as the increment of the intracellular concentration of Na+ can directly lead to the enhancement of muscular aerobic glycolysis and its mechanism plays a significant role in the enhancement of muscular glycolysis in septic states.
Chai
J
J
Burn Institute PLA 304th Hospital, Beijing 100037, China.
Diao
L
L
Sheng
Z
Z
D000818:Animals; D006019:Glycolysis / Q000502:physiology*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008985:Monensin / Q000494:pharmacology; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D019344:Lactic Acid; D008985:Monensin; D012964:Sodium