[Studies on the properties of dendritic cells from cord blood CD34+ cells and peripheral blood monocytes].
OBJECTIVE
Ex vivo expansion and purification of dendritic cells(DC) with typical phenotype, morphology and function are critical to the further studies on DC and their clinical application.


METHODS
CD34+ cells were isolated from umbilical cord blood by using a high-gradient magnetic cell sorting system (MACS), and peripheral blood mononuclear cells (PBMC) were depleted of T and B cells by mixing them with T/CD2 and B/CD19 magnetic beads. DC were induced and expanded with different combinations of cytokines. They were identified for their properties by FACS, electronic microscopy, microscopy, and mixed lymphocyte reaction (MLR).


RESULTS
Cultures of cord blood CD34+ cells with the combination of GM-CSF + TNF-alpha + SCF + FL yielded (27.18 +/- 1.56)% CD1a+ cells, much higher than that (0.65 +/- 0.38)% with GM-CSF alone. The combination of GM-CSF and high dose IL-4(1,000 U/ml) was the most potential for expanding CD1a+ cells [(21.8 +/- 0.32)%] for PBMC. Both of the DC from different sources were similar in phenotype and morphology, and had the capacity to stimulate proliferation of allogeneic T lymphocytes.


CONCLUSIONS
Both of cord blood and peripheral blood are the source for generation of a large number of typical DC, and can be adopted for the application according to different purposes. These results lay foundation for the immunotherapy with DC.
Wang
X
X
Beijing Institute of Radiation Medicine, Beijing 100850.
Pei
X
X
Li
L
L
D018952:Antigens, CD34 / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype
D018952:Antigens, CD34