Vitamin C inhibited DNA adduct formation and arylamine N-acetyltransferase activity and gene expression in rat glial tumor cells.
Studies have been demonstrated that vitamin C (ascorbic acid) exhibit the protective role of vin in certain types of cancer. Rat glial tumor cells also have been shown have N-acetyltransferase activity. In this study, we reported the effects of vitamin C on arylamine N-acetyltransferase (NAT) activity and DNA adduct formation in rat glial tumor cell line (C6 glioma). The activity of NAT was measured by high performance liquid chromatography assaying for the amounts of acetylated 2-aminofluorene and p-aminobenzoic acid and nonacetylated 2-aminofluorene and p-amonibenzoic acid. Rat C6 glioma cells were used for examining NAT activity and gene expression and 2-aminofluorene-DNA adduct formation. The results demonstrated that NAT activity and 2-aminofluorene-DNA adduct formation in C6 glioma cells were inhibited and decreased by vitamin C in a dose-dependent manner. But vitamin C did not affect NAT gene expression in examined cells. The apparent kinetic parameters (apparent values of Km and Vmax) from C6 glioma cells were also determined with or without vitamin C cotreatment. The data also indicated that vitamin C decreased the apparent values of Km and Vmax from C6 glioma cells.
Hung
C F
CF
Department of Surgery, Jen-Ai Hospital, Taichung, Taiwan, Republic of China.
Lu
K H
KH
D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D000107:Acetylation; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D001932:Brain Neoplasms; D018736:DNA Adducts / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D005449:Fluorenes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma; D007700:Kinetics; D051381:Rats; D014407:Tumor Cells, Cultured
D018736:DNA Adducts; D005449:Fluorenes; C012177:2-aminofluorene; D004492:Edetic Acid; D001191:Arylamine N-Acetyltransferase; D001205:Ascorbic Acid; D010129:4-Aminobenzoic Acid