Trypanosoma evansi: demonstration of a transferrin receptor derived from expression site-associated genes 6 and 7.
In Trypanosoma brucei, uptake of host transferrin is mediated by a heterodimeric, glycosylphosphatidylinositol-anchored receptor derived from the 2 expression site-associated genes 6 and 7 (ESAG6 and ESAG7). By using specific antibodies, it is shown here that T. evansi, a trypanosome species transmitted mechanically by biting flies, also expresses a transferrin receptor composed of ESAG6 and ESAG7. The cellular uptake of transferrin in T. evansi is completely inhibited with anti-T. brucei (ESAG6/7 heterodimer) antibodies. The demonstration of a functional ESAG6/7 transferrin receptor in T. evansi supports further its close relationship to T. brucei.
Kabiri
M
M
Abteilung Parasitologie, Hygiene-Institut der Ruprecht-Karls-Universität, Heidelberg, Germany.
Steverding
D
D
D000818:Animals; D000913:Antibodies, Protozoan / Q000737:chemistry / Q000494:pharmacology; D015153:Blotting, Western; D016054:DNA, Protozoan / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000737:chemistry; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D011990:Receptors, Transferrin / Q000235:genetics* / Q000378:metabolism; D014168:Transferrin / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics; D014345:Trypanosoma / Q000235:genetics* / Q000378:metabolism
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; C090143:ESAG 6 protein, Trypanosoma brucei; D006023:Glycoproteins; D011480:Protease Inhibitors; D015800:Protozoan Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; C078011:ESAG 7 protein, Trypanosoma brucei