title: Postnatal development of hepatocellular apolipoprotein B assembly and secretion in the rat.
abstract: In this study, we explored the paradox that in suckling rats the serum concentration of LDL is high although the liver secretes only minimal quantities of VLDL, the presumed precursor of LDL. Freshly isolated hepatocytes and hepatocytes in primary culture obtained from adult (90 days old) and suckling (17 days old) rats were used to investigate the synthesis and secretion of apolipoprotein B (apoB) and lipids as well as the density profile of secreted apoB-containing lipoproteins. Furthermore, the effects of dexamethasone and oleate on apoB biogenesis were investigated in primary cultures of hepatocytes from adult and suckling rats. Hepatocytes from suckling rats were unable to assemble mature VLDL but secreted apoB as primordial lipoprotein particles in the LDL-HDL density range. Intracellular degradation of apoB was also reduced in hepatocytes from suckling rats compared with that in hepatocytes from adults. The immaturity in VLDL assembly and apoB degradation of hepatocytes from suckling rats could be overcome by treating the cultures with dexamethasone plus oleate or dexamethasone alone. The lower microsomal triacylglycerol transfer protein (MTP) mRNA concentrations in hepatocytes from suckling rats in comparison with hepatocytes from adult rats were not reflected in lower MTP activity levels. Furthermore, dexamethasone plus oleate treatment had no effect on MTP activity although VLDL assembly and secretion were clearly stimulated. We conclude that, during the suckling period of the rat, serum LDL is directly produced by the liver. This is a result of impaired hepatic VLDL assembly, which is a consequence of low triglyceride synthesis and an inefficient mobilization of bulk lipids in the second step of VLDL assembly.
authors.affiliation: Institute of Pathobiochemistry, Department of Medicine, Friedrich-Schiller University, 07740 Jena, Germany. dplo@mti-n.uni-jena.de
authors.forename: D
authors.initials: D
authors.lastname: Plonné
authors.forename: H P
authors.initials: HP
authors.lastname: Schulze
authors.forename: U
authors.initials: U
authors.lastname: Kahlert
authors.forename: K
authors.initials: K
authors.lastname: Meltke
authors.forename: H
authors.initials: H
authors.lastname: Seidolt
authors.forename: A J
authors.initials: AJ
authors.lastname: Bennett
authors.forename: I J
authors.initials: IJ
authors.lastname: Cartwright
authors.forename: J A
authors.initials: JA
authors.lastname: Higgins
authors.forename: U
authors.initials: U
authors.lastname: Till
authors.forename: R
authors.initials: R
authors.lastname: Dargel
mesh_terms: D000375:Aging; D000818:Animals; D000833:Animals, Suckling; D001055:Apolipoproteins B / Q000096:biosynthesis* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000096:biosynthesis / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D008862:Microsomes, Liver / Q000737:chemistry; D019301:Oleic Acid / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D001055:Apolipoproteins B; D002352:Carrier Proteins; D005938:Glucocorticoids; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D012333:RNA, Messenger; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein; D019301:Oleic Acid; D003907:Dexamethasone