title: Long-term follow-up failed to detect in vitro transmission of full-length porcine endogenous retroviruses from specific pathogen-free pig islets to human cells.
abstract: OBJECTIVE
  Islets from specific pathogen-free (SPF) pigs could prevent the transmission of conventional zoonosis, but not endogenous retroviruses (PERV), from pigs to diabetic patients. We previously reported that the pancreas showed the lowest expression of PERV mRNA among pig tissues intended for grafting. This study aimed to determine whether PERV from pig islets infect human cells during co-incubation.
  
  
  METHODS
  Human cells (including highly PERV-sensitive 293 cells) were incubated with SPF pig islet cells under conditions designed to increase contact (a high islet to human cell ratio, extended period of co-culture, and repeated contacts). PK15 and G2 retrovirus-producing pig cells were used in place of islet cells as "positive infection controls". Infection of human cells was monitored on cellular extracts and supernatants by PCR or long PCR, and RT-PCR or long RT-PCR, to detect PERV DNA and mRNA, respectively. Reverse-transcriptase activity was monitored by PERT.
  
  
  RESULTS
  Despite the presence of all PERV sequences in pig islet cells, including full-length inserts, no DNA or RNA for gag, pol, and the 3 env sub-types were detected in any human cell line or blood mononuclear cells incubated with pig islet cells, during an 18-week follow-up period. No PERV sequences or RT activity were detected in supernatants. PERV signals were negative even when the pig islet to human cell ratio was increased to 100:1, the time of co-culture was extended to 5 days and two sequential co-incubations were done. By contrast, all PERV DNA and mRNA were detected in all human cells co-incubated with PK15 or G2 cells. Depending on human cell types, productive or non-productive infections were obtained: full-length PERV RNA and RT activity in supernatants were detected or not; and PERV sequences to previously unexposed human cells by PERV-infected human cells were transmitted or not. Some human cells were not productively infected by PK15 cells but became productively infected after co-incubation with PERV-infected 293 cells.
  
  
  CONCLUSIONS
  SPF pig islet cells, even with PERV inserts and transcripts, have very little probability of transmitting PERV to human cells during co-incubation. The sensitivity of human cells to stable and productive infection by PERV depends on the cell type. Human adaptation of PERV was observed.
authors.affiliation: Cellular and Molecular Immuno-Endocrinology, INRA/ENVN/University, Nantes cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Clémenceau
authors.forename: D
authors.initials: D
authors.lastname: Jégou
authors.forename: L
authors.initials: L
authors.lastname: Martignat
authors.forename: P
authors.initials: P
authors.lastname: Saï
mesh_terms: D000818:Animals; D002460:Cell Line; D005500:Follow-Up Studies; D006801:Humans; D007515:Islets of Langerhans / Q000821:virology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D019169:Jurkat Cells; D020014:K562 Cells; D012192:Retroviridae Infections / Q000635:transmission* / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015047:Zoonoses