Protective effects of curcumin against oxidative damage on skin cells in vitro: its implication for wound healing.
BACKGROUND
Curcumin, isolated from turmeric, has been known to possess many pharmacologic properties. It has been proven to exhibit remarkable anticarcinogenic, anti-inflammatory, and antioxidant properties. Turmeric curcumin may be a good potential agent for wound healing.


METHODS
To further understand its therapeutic mechanisms on wound healing, the antioxidant effects of curcumin on hydrogen peroxide (H2O2) and hypoxanthine-xanthine oxidase induced damage to cultured human keratinocytes and fibroblasts were investigated. Cell viability was assessed by colorimetric assay and quantification of lactate dehydrogenase release.


RESULTS
Exposure of human keratinocytes to curcumin at 10 microg/mL showed significant protective effect against hydrogen peroxide. Interestingly, exposure of human dermal fibroblasts to curcumin at 2.5 microg/mL showed significant protective effects against hydrogen peroxide. No protective effects of curcumin on either fibroblasts or keratinocytes against hypoxanthine-xanthine oxidase induced damage were found in our present studies.


CONCLUSIONS
The findings indicate that curcumin indeed possessed powerful inhibition against hydrogen peroxide damage in human keratinocytes and fibroblasts.
Phan
T T
TT
Department of Plastic Surgery/National Burns Centre, Singapore General Hospital. gplptt@sgh.com.sg
See
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P
Lee
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Chan
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D000704:Analysis of Variance; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003474:Curcumin / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D016877:Oxidants / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D014969:Xanthine Oxidase / Q000633:toxicity
D000975:Antioxidants; D004791:Enzyme Inhibitors; D016877:Oxidants; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D014969:Xanthine Oxidase; D003474:Curcumin