title: Normal human lung fibroblasts differently modulate interleukin-10 and interleukin-12 production by monocytes: implications for an altered immune response in pulmonary chronic inflammation.
abstract: The ability of lung fibroblasts to modulate the immune response has been evaluated by analyzing the synthesis and release of interleukin (IL)-10 and IL-12 by lipopolysaccharide (LPS)-stimulated peripheral blood monocytes exposed to pulmonary fibroblast conditioned medium (FCM). IL-10 and IL-12 contents and gene expression were markedly modified by treatment with FCM as measured by ELISA (+97.5 +/- 12.8% and -68 +/- 7.3% for IL-10 and IL-12, respectively), immunocytochemistry, and reverse transcriptase-polymerase chain reaction (RT-PCR). These effects appeared to be mediated by prostaglandin E(2) (PGE(2)) as the modified release of both cytokines was reduced by treatment with indomethacin and mimicked by addition of exogenous PGE(2.) As a result of the enhanced production of IL-10, exposure of LPS/interferon (IFN)-gamma-activated monocytes to FCM was also able to reduce the expression of the class II major histocompatibility complex (MHC) molecule, human leukocyte-associated antigen-DR (HLA-DR) (-51.8 +/- 8.7%) and of the costimulatory molecule, CD40 (-53.9 +/- 11.7%). The expression of both molecules was completely restored when monocytes were pretreated with a neutralizing anti-IL-10 monoclonal antibody. The FCM obtained from fibrotic lung fibroblasts was instead less efficacious in potentiating LPS-stimulated IL-10 release and, consequently, in reducing HLA-DR and CD40 expression, suggesting that an impairment of the immune regulation operated by fibroblasts may be involved in the maintenance of chronic pulmonary inflammation.
authors.affiliation: Institutes of Respiratory and Infectious Diseases and Department of Experimental and Clinical Pharmacology, University of Catania, Catania, Italy. vancheri@mbox.unict.it
authors.forename: C
authors.initials: C
authors.lastname: Vancheri
authors.forename: C
authors.initials: C
authors.lastname: Mastruzzo
authors.forename: V
authors.initials: V
authors.lastname: Tomaselli
authors.forename: M A
authors.initials: MA
authors.lastname: Sortino
authors.forename: L
authors.initials: L
authors.lastname: D'Amico
authors.forename: G
authors.initials: G
authors.lastname: Bellistrí
authors.forename: M P
authors.initials: MP
authors.lastname: Pistorio
authors.forename: E T
authors.initials: ET
authors.lastname: Salinaro
authors.forename: F
authors.initials: F
authors.lastname: Palermo
authors.forename: A
authors.initials: A
authors.lastname: Mistretta
authors.forename: N
authors.initials: N
authors.lastname: Crimi
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D019013:CD40 Antigens / Q000032:analysis / Q000096:biosynthesis; D002450:Cell Communication / Q000276:immunology; D002478:Cells, Cultured; D002908:Chronic Disease; D015232:Dinoprostone / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D015870:Gene Expression / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics*; D018664:Interleukin-12 / Q000032:analysis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000166:cytology* / Q000276:immunology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D019013:CD40 Antigens; D006684:HLA-DR Antigens; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016753:Interleukin-10; D018664:Interleukin-12; D015232:Dinoprostone; D007213:Indomethacin