title: Cloning and sequencing of the fragment of angiostatin K (1-3) gene.
abstract: OBJECTIVE
  To secure anti-endotheliocyte hyperplasia functional fragment of human angiostatin K (1-3) gene.
  
  
  METHODS
  As the template of leukocytotic cDNA, functional fragment of human angiostatin K (1-3) cDNA was amplified by PCR, cloned into the vector pGEM-3Zf, and sequenced according to Dye primer sequencing kit.
  
  
  RESULTS
  The functional fragment of angiostatin K (1-3) cDNA (859 bp) was obtained by PCR and determined by sequencing.
  
  
  CONCLUSIONS
  The angiostatin K (1-3) gene has been cloned. The gene is of great significance in tumor-antiangiogenesis therapy.
authors.affiliation: Institute of Neurosurgery of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Chai
authors.forename: Z
authors.initials: Z
authors.lastname: Fei
authors.forename: L
authors.initials: L
authors.lastname: Yi
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Liang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000235:genetics*; D043168:Angiostatins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D010958:Plasminogen / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020533:Angiogenesis Inhibitors; D010446:Peptide Fragments; D043168:Angiostatins; D010958:Plasminogen; D004247:DNA