Applications of single-strand conformation polymorphism (SSCP) to taxonomy, diagnosis, population genetics and molecular evolution of parasitic nematodes.
The analysis of genetic variation in parasitic nematodes has important implications for studying aspects of taxonomy, diagnosis, population genetics, drug resistance and molecular evolution. This article highlights some applications of PCR-based single-strand conformation polymorphism (SSCP) for the analysis of sequence variation in individual parasites (and their populations) to address some of these areas. It also describes the principles and advantages of SSCP, and provides some examples for future applications in parasitology.
Gasser
R B
RB
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au
Chilton
N B
NB
D000818:Animals; D004275:DNA, Ribosomal / Q000032:analysis*; D004351:Drug Resistance / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D005828:Genetics, Population; D009154:Mutation; D009348:Nematoda / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D009349:Nematode Infections / Q000175:diagnosis / Q000469:parasitology* / Q000628:therapy; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D018807:Polymorphism, Single-Stranded Conformational
D004275:DNA, Ribosomal