title: Fc receptor-mediated immunity against Bordetella pertussis.
abstract: The relevance of specific Abs for the induction of cellular effector functions against Bordetella pertussis was studied. IgG-opsonized B. pertussis was efficiently phagocytosed by human polymorphonuclear leukocytes (PMN). This process was mediated by the PMN IgG receptors, FcgammaRIIa (CD32) and FcgammaRIIIb (CD16), working synergistically. Furthermore, these FcgammaR triggered efficient PMN respiratory burst activity and mediated transfer of B. pertussis to lysosomal compartments, ultimately resulting in reduced bacterial viability. Bacteria opsonized with IgA triggered similar PMN activation via FcalphaR (CD89). Simultaneous engagement of FcalphaRI and FcgammaR by B. pertussis resulted in increased phagocytosis rates, compared with responses induced by either isotype alone. These data provide new insights into host immune mechanisms against B. pertussis and document a crucial role for Ig-FcR interactions in immunity to this human pathogen.
authors.affiliation: Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands. mer@quimica.unlp.edu.ar
authors.forename: M E
authors.initials: ME
authors.lastname: Rodriguez
authors.forename: S M
authors.initials: SM
authors.lastname: Hellwig
authors.forename: D F
authors.initials: DF
authors.lastname: Hozbor
authors.forename: J
authors.initials: J
authors.lastname: Leusen
authors.forename: W L
authors.initials: WL
authors.lastname: van der Pol
authors.forename: J G
authors.initials: JG
authors.lastname: van de Winkel
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000502:physiology; D001770:Blood Bactericidal Activity; D001886:Bordetella pertussis / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D007070:Immunoglobulin A / Q000097:blood / Q000502:physiology; D007074:Immunoglobulin G / Q000097:blood / Q000502:physiology; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D009895:Opsonin Proteins / Q000097:blood / Q000502:physiology; D010587:Phagocytosis / Q000276:immunology; D011961:Receptors, Fc / Q000097:blood / Q000502:physiology*; D017452:Receptors, IgG / Q000097:blood / Q000502:physiology; D016897:Respiratory Burst / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D011961:Receptors, Fc; D017452:Receptors, IgG