Chromosome and oxidative damage biomarkers in lymphocytes of Parkinson's disease patients.
As cancer development usually results from exposure to several environmental risk factors in interaction with the genetic susceptibility of the host, it could be of interest to investigate if neurodegeneration, as occurs in Parkinson's disease (PD) patients can be attributed at least partially, to environmental risk factors. There is growing evidence that oxidative stress could play a significant role as a risk factor in the aetiology and pathogenesis of neurodegenerative diseases, emphasising the need for new individual and human-based approaches. The aim of our research is to explore the relation between chromosome instability and oxidative stress biomarkers in Parkinson's disease using a variety of strategies. We determined peripheral markers for oxidative damage in PD by testing for spontaneous and induced chromosomal damage, DNA strand breaks, oxidised pyrimidines and altered purines both in peripheral blood and cultured lymphocytes. We also measured glutathione S-transferase activity in the plasma of patients and controls. Compared to healthy controls, PD patients show higher frequencies of micronuclei (17.2 +/- 4.8 vs. 9.0 +/- 3.4, p < 0.001) and a significant increase in the levels of single strand breaks (SSB). Significant differences were also obtained in the distribution of oxidised purine bases between the two groups. Preliminary data obtained by fluorescence in situ hybridization analysis showed that the percentage of centromere negative micronuclei is higher than that of centromere positive micronuclei. Glutathione S-transferase activity in plasma from PD patients and controls was also measured and the enzymatic activity in PD patients was lower than in healthy controls.
Migliore
L
L
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Via S. Giuseppe 22, 56026 Pisa, Italy. l.migliore@geog.unipi.it
Scarpato
R
R
Coppede
F
F
Petrozzi
L
L
Bonuccelli
U
U
Rodilla
V
V
D000368:Aged; D015415:Biomarkers / Q000032:analysis*; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D002869:Chromosome Aberrations; D004249:DNA Damage; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D018384:Oxidative Stress; D010300:Parkinson Disease / Q000503:physiopathology*; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012307:Risk Factors
D015415:Biomarkers; D011687:Purines; D011743:Pyrimidines; D005982:Glutathione Transferase