Effects of acid inhibition on somatostatin-producing cells in the rat gastric fundus.
Somatostatin plays a role in the regulation of gastric acid secretion. Omeprazole, a potent inhibitor of gastric acid secretion, has been reported to cause either a significant decrease or increase in the formation of gastric somatostatin-producing cells. Therefore, we determined in the present study distribution patterns of somatostatin mRNA and protein in fundus mucosa of rats after long-term inhibition of gastric acid secretion. Female Sprague-Dawley rats were given 0, 20 and 100 mg/kg/day omeprazole, respectively, as gastric instillations during 2 months. Serum gastrin levels were significantly higher in the third group than in the other groups. The omeprazole-treated groups also showed an increase in the number of somatostatin-containing cells in fundus mucosa. Moreover, the intensity of somatostatin-positivity was higher in the treated groups than in the control group. We also observed an increase in the number of cells containing somatostatin mRNA in fundus mucosa of omeprazole-treated rats. These results suggest that long-term inhibition of acid secretion does not inhibit but stimulate somatostatin production in mucosa of rat gastric fundus.
Bolkent
S
S
Department of Medical Biology and Genetics, Faculty of Dentistry, Marmara University, Istanbul, Turkey. sbolkent@superonline.com
Yilmazer
S
S
Kaya
F
F
Oztürk
M
M
D000818:Animals; D005260:Female; D005744:Gastric Acid / Q000378:metabolism; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000097:blood; D005786:Gene Expression Regulation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D009853:Omeprazole / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013004:Somatostatin / Q000096:biosynthesis* / Q000235:genetics
D005755:Gastrins; D012333:RNA, Messenger; D013004:Somatostatin; D009853:Omeprazole