Cutting edge: IL-4 receptor expression by non-bone marrow-derived cells is required to expel gastrointestinal nematode parasites.
Expulsion of two gastrointestinal nematode parasites, Nippostrongylus brasiliensis and Trichinella spiralis, is similar in that both require IL-4Ralpha expression, but different in that T cells and mast cells are required for IL-4-induced expulsion of T. spiralis but not N. brasiliensis. To examine the role of IL-4Ralpha signaling in immunity to these parasites, we studied worm expulsion in chimeric mice that selectively expressed IL-4Ralpha on bone marrow-derived or non-bone marrow-derived cells. N. brasiliensis was expelled by mice that expressed IL-4Ralpha only on non-bone marrow-derived cells, but not by mice that expressed IL-4Ralpha only on bone marrow-derived cells. Although T. spiralis expulsion required IL-4Ralpha expression by both bone marrow- and non-bone marrow-derived cells, IL-4 stimulation eliminated the requirement for IL-4Ralpha expression by bone marrow-derived cells. Thus, direct IL-4Ralpha signaling of nonimmune gastrointestinal cells may be generally required to induce worm expulsion, even when mast cell and T cell responses are also required.
Urban
J F
JF
U.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Immunology and Disease Resistance Laboratory, Beltsville, MD 20705, USA.
Noben-Trauth
N
N
Schopf
L
L
Madden
K B
KB
Finkelman
F D
FD
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001854:Bone Marrow Cells / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D005260:Female; D005767:Gastrointestinal Diseases / Q000276:immunology* / Q000469:parasitology* / Q000517:prevention & control; D015847:Interleukin-4 / Q000502:physiology; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology / Q000469:parasitology; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008819:Mice, Nude; D016513:Mice, SCID; D009559:Nippostrongylus / Q000276:immunology*; D019948:Receptors, Interleukin-4 / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D017206:Strongylida Infections / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control
D019948:Receptors, Interleukin-4; D015847:Interleukin-4