title: Isolation, characterization, and polyaromatic hydrocarbon degradation potential of aerobic bacteria from marine macrofaunal burrow sediments and description of Lutibacterium anuloederans gen. nov., sp. nov., and Cycloclasticus spirillensus sp. nov.
abstract: Two new polyaromatic hydrocarbon-degrading marine bacteria have been isolated from burrow wall sediments of benthic macrofauna by using enrichments on phenanthrene. Strain LC8 (from a polychaete) and strain M4-6 (from a mollusc) are aerobic and gram negative and require sodium chloride (>1%) for growth. Both strains can use 2- and 3-ring polycyclic aromatic hydrocarbons as their sole carbon and energy sources, but they are nutritionally versatile. Physiological and phylogenetic analyses based on 16S ribosomal DNA sequences suggest that strain M4-6 belongs to the genus Cycloclasticus and represents a new species, Cycloclasticus spirillensus sp. nov. Strain LC8 appears to represent a new genus and species, Lutibacterium anuloederans gen. nov., sp. nov., within the Sphingomonadaceae. However, when inoculated into sediment slurries with or without exogenous phenanthrene, only L. anuloederans appeared to sustain a significant phenanthrene uptake potential throughout a 35-day incubation. In addition, only L. anuloederans appeared to enhance phenanthrene degradation in heavily contaminated sediment from Little Mystic Cove, Boston Harbor, Boston, Mass.
authors.affiliation: Darling Marine Center, University of Maine, Walpole 04573, USA.
authors.forename: W K
authors.initials: WK
authors.lastname: Chung
authors.forename: G M
authors.initials: GM
authors.lastname: King
mesh_terms: D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D004275:DNA, Ribosomal / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D008974:Mollusca / Q000502:physiology; D009281:Naphthalenes / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D011077:Polychaeta / Q000502:physiology; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D009281:Naphthalenes; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; C031721:naphthalene; C031181:phenanthrene