title: Oxidative stress and the mechanical properties of naturally occurring chimeric collagen-containing fibers.
abstract: The byssal threads of marine mussels are a fiber-reinforced composite material. Fibers are continuous, separated by matrix, and consist of chimeric collagens that encompass within the same primary protein structure domains corresponding to collagen, polyhistidine, and either elastin or dragline spider silk. The elastic modulus (stiffness) of the proximal portion of byssal threads was measured by cyclic stress-strain analysis at 50% extension. Before measurement, the threads were conditioned by various treatments, particularly agitation in aerated or nitrogen-sparged seawater. Stiffness can be permanently increased by more than two times, e.g., from 25 MPa to a maximum of 65 MPa, by simple agitation in aerated seawater. Much but not all of this stiffening can be prevented by agitation under nitrogen. Reversible strain stiffening would seem to be a useful adaptation to lower residual stresses arising from the deformation of two joined materials, i.e., distal and proximal portions with rather different elastic moduli. The permanent strain stiffening that characterizes proximal byssal threads subjected to oxidative stress is probably due to protein cross-linking. In the short term, this results in a stronger thread but at the expense of dynamic interactions between the molecules in the structure.
authors.affiliation: Marine Science Institute and MCDB Department, University of California at Santa Barbara, Santa Barbara, California 93106, USA.
authors.forename: C
authors.initials: C
authors.lastname: Sun
authors.forename: E
authors.initials: E
authors.lastname: Vaccaro
authors.forename: J H
authors.initials: JH
authors.lastname: Waite
mesh_terms: D000818:Animals; D049872:Bivalvia; D003094:Collagen / Q000737:chemistry*; D004548:Elasticity; D014442:Monophenol Monooxygenase / Q000737:chemistry; D018384:Oxidative Stress; D017434:Protein Structure, Tertiary; D012623:Seawater / Q000737:chemistry; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D003094:Collagen; D014442:Monophenol Monooxygenase