A partial BRCA1 sequence homology mapping to 4q28.
Using a BRCA1 cDNA probe in Southern analysis, we detected a sequence of 348 bp on 4q28 that is homologous to the 3' end of BRCA1. A 28-kb sequence contig has been assembled spanning the homologous region, which we designated BRCA1-h. An open reading frame was identified encoding a sequence of 82 amino acids; 22 of the last 23 amino acids are identical to the last 23 residues of BRCA1. BLAST-searches, RT-PCR and RACE-experiments have been unable to provide evidence that BRCA1-h is part of an expressed gene.
Stec
I
I
Department of Human and Clinical Genetics, Leiden University Medical Center, Leiden, The Netherlands. I.Stec@LUMC.nl
van Vliet
M
M
van Eijk
R
R
Meijers
H
H
Kroeze
K H
KH
Dauwerse
J G
JG
van Ommen
G J
GJ
Cornelisse
C J
CJ
den Dunnen
J T
JT
Devilee
P
P
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D020451:Contig Mapping; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D019398:Genes, BRCA1; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid