title: Up-regulation of phosphatidylinositol 3-kinase in psoriatic lesions.
abstract: OBJECTIVE
  To explore the relationship between phosphatidylinositol 3-kinase (PI 3-kinase) and psoriasis.
  
  
  METHODS
  We constructed eukaryotic expression vector PcDNA 3-PI 3-kinase, by which digoxin-labeled RNA probe of PI 3-kinase was generated. After that, we detected the expressions of PI 3-kinase in the skins of twelve patients with plaque psoriasis and five normal subjects by dot blot, in situ hybridization and immunohistochemistry.
  
  
  RESULTS
  The expression of PI 3-kinase mRNA and protein in psoriatic lesions increased obviously compared with that in normal skin. The expression of the PI 3-kinase gene conformed to the expression of its protein in psoriatic and normal human skins.
  
  
  CONCLUSIONS
  Over-expression of PI 3-kinase may be related to the hyperproliferation of psoriatic keratinocytes. Transfection of the anti-sense gene of PI 3-kinase into psoriatic keratinocytes might be a best way to elucidate the issue.
authors.affiliation: Department of Biology, Beijing Normal University, Beijing 100875, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis* / Q000235:genetics; D011565:Psoriasis / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000201:enzymology* / Q000473:pathology; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases