title: The role of PDR13 in tolerance to high copper stress in budding yeast.
abstract: PDR13 in Saccharomyces cerevisiae contributes to drug resistance via sequential activation of PDR1 and PDR5. In this study, we found that a PDR13 deletion mutant was hypersensitive to Cu(2+) compared to the wild-type counterpart. The Cu(2+) tolerance mechanism mediated by Pdr13 does not seem to involve Pdr1 or Pdr5, since mutants harboring a deletion of either the PDR1 or PDR5 gene did not show elevated Cu(2+) sensitivity. Instead, we found that the PDR13 null mutant could not express CUP1 or CRS5 metallothionein at wild-type levels when subjected to high Cu(2+) stress. These results suggest that Pdr13 contributes to high Cu(2+) tolerance of S. cerevisiae, at least in part, via a mechanism involving metallothionein expression.
authors.affiliation: Division of Molecular Life Sciences, Pohang University of Science and Technology, Pohang 790-784, South Korea.
authors.forename: D Y
authors.initials: DY
authors.lastname: Kim
authors.forename: W Y
authors.initials: WY
authors.lastname: Song
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism; D002352:Carrier Proteins; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D005800:Genes, Fungal; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D018528:ATP-Binding Cassette Transporters; C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C085480:PDR1 protein, S cerevisiae; C092438:PDR5 protein, S cerevisiae; C424445:SSZ1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C007121:copper thionein; D002104:Cadmium; D003300:Copper; D008668:Metallothionein