title: Blood-sucking lice may disseminate Trypanosoma cruzi infection in baboons.
abstract: Trypanosoma cruzi (Schyzotrypanum, Chagas, 1909), and Chagas disease are endemic in captive-reared baboons at the Southwest Foundation for Biomedical Research, San Antonio, Texas. We obtained PCR amplification products from DNA extracted from sucking lice collected from the hair and skin of T. cruzi-infected baboons, with specific nested sets of primers for the protozoan kinetoplast DNA, and nuclear DNA. These products were hybridized to their complementary internal sequences. Selected sequences were cloned and sequencing established the presence of T. cruzi nuclear DNA, and minicircle kDNA. Competitive PCR with a kDNA set of primers determined the quantity of approximately 23.9 +/- 18.2 T. cruzi per louse. This finding suggests that the louse may be a vector incidentally contributing to the dissemination of T. cruzi infection in the baboon colony.
authors.affiliation: Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasília, Brazil.
authors.forename: E R
authors.initials: ER
authors.lastname: Argañaraz
authors.forename: G B
authors.initials: GB
authors.lastname: Hubbard
authors.forename: L A
authors.initials: LA
authors.lastname: Ramos
authors.forename: A L
authors.initials: AL
authors.lastname: Ford
authors.forename: N
authors.initials: N
authors.lastname: Nitz
authors.forename: M M
authors.initials: MM
authors.lastname: Leland
authors.forename: J L
authors.initials: JL
authors.lastname: Vandeberg
authors.forename: A R
authors.initials: AR
authors.lastname: Teixeira
mesh_terms: D000818:Animals; D018105:DNA, Kinetoplast / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004199:Disease Vectors; D010373:Lice Infestations / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D010215:Papio / Q000469:parasitology*; D008003:Phthiraptera / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan