title: Identification of Hake species (Merluccius Genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences.
abstract: The use of DNA-based methodologies in identification of hake species belonging to the Merluccius genus was shown to be successful. A short fragment of the left hypervariable domain of the mitochondrial control region was amplified, sequenced, and digested from 11 hake species. The hake-specific PCR product, due to its limited size, was obtained in a variety of tissue samples with different levels of DNA concentration and degradation, including sterilized food products. On the basis of this phylogenetically informative 156-bp sequence were selected four restriction enzymes (ApoI, DdeI, DraIII, and MboII) that allow the hake species discrimination. Species identification by phylogenetic analysis of sequences or by PCR-RFLP methodologies is useful in a variety of scenarios including authentication of thermally processed food, detection of food components, and species determination of individuals whose morphological characters are removed.
authors.affiliation: Departamento de Bioquímica e Bioloxía Molecular, Facultade de Bioloxía, Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Spain. bnjquint@usc.es
authors.forename: J
authors.initials: J
authors.lastname: Quinteiro
authors.forename: R
authors.initials: R
authors.lastname: Vidal
authors.forename: M
authors.initials: M
authors.lastname: Izquierdo
authors.forename: C G
authors.initials: CG
authors.lastname: Sotelo
authors.forename: M J
authors.initials: MJ
authors.lastname: Chapela
authors.forename: R I
authors.initials: RI
authors.lastname: Pérez-Martín
authors.forename: H
authors.initials: H
authors.lastname: Rehbein
authors.forename: G L
authors.initials: GL
authors.lastname: Hold
authors.forename: V J
authors.initials: VJ
authors.lastname: Russell
authors.forename: S E
authors.initials: SE
authors.lastname: Pryde
authors.forename: C
authors.initials: C
authors.lastname: Rosa
authors.forename: A T
authors.initials: AT
authors.lastname: Santos
authors.forename: M
authors.initials: M
authors.lastname: Rey-Méndez
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D005399:Fishes / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial