Antibody engineering.
Antibodies are unique in their high affinity and specificity for a binding partner, a quality that has made them one of the most useful molecules for biotechnology and biomedical applications. The field of antibody engineering has changed rapidly in the past 10 years, fueled by novel technologies for the in vitro isolation of antibodies from combinatorial libraries and their functional expression in bacteria. This review presents an overview of the methods available for the de novo generation of human antibodies, for engineering antibodies with increased antigen affinity, and for the production of antibody fragments. Select applications of recombinant antibodies are also presented.
Maynard
J
J
Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712, USA.
Georgiou
G
G
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000235:genetics*; D000915:Antibody Affinity; D001698:Biomedical Engineering; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008958:Models, Molecular; D009369:Neoplasms / Q000628:therapy; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D019151:Peptide Library; D011994:Recombinant Proteins