[The effect of EBNA-1 ribozyme on the ability of tumor genesis of lymphoblastoid cell lines in SCID mice].
OBJECTIVE
To explore the effect of EBNA-1 specific ribozyme on the tumor genesis ability of lymphoblastoid cell lines (LCLs) in severe combined immunodeficiency (SCID) mice.


METHODS
Using molecular cloning technique, recombinant adenovirus vectors expressing EBNA-1 ribozyme (RZ1) or its inactive mutant (RZ1mut) were constructed as Ad.RZ1 or Ad.RZ1mut. Recombinant adenoviruses were generated by homologous recombination and isolated by plaque formation. LCLs were established and infected with recombinant adenovirus and then injected in SCID mice (1 x 10(7) cells/animal). The SCID mice were sacrificed six weeks after injection, then the tumors were excised from these mice and their size and weight were measured. The expression of EBNA-1 ribozyme and EBNA-1 mRNA in the tumor was analyzed by RT-PCR, and the expression of EBNA-1 protein in the tumor was detected by Western blot.


RESULTS
The tumors (0.27 +/- 0.18) g formed from the Ad.RZ1-treated LCLs were much smaller than those of the untreated LCLs (1.04 +/- 0.27) g or LCLs treated with control vector of adenovirus (1.12 +/- 0.32) g (P < 0.01), and also smaller than those of Ad.RZ1mut-treated LCLs (0.76 +/- 0.28) g (P < 0.05). The expression of EBNA-1 mRNA and protein in the tumors of Ad.RZ1-treated LCLs decreased significantly than that of other groups.


CONCLUSIONS
Adenovirus delivery of EBNA-1 ribozyme was able to suppress LCL tumors significantly and depress the tumor genesis ability of B lymphocyte induced by EBV.
Liu
A
A
Department of Respiratory Medicine, Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Jiang
Y
Y
Zhao
K
K
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D015197:Carcinogenicity Tests; D002478:Cells, Cultured; D003001:Cloning, Molecular; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D016513:Mice, SCID; D016337:RNA, Catalytic / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology
D019309:Epstein-Barr Virus Nuclear Antigens; D016337:RNA, Catalytic; D012333:RNA, Messenger; D011994:Recombinant Proteins; C417029:EBV-encoded nuclear antigen 1