A stabilized flavonoid glycoside in heat-treated Cassia alata leaves and its structural elucidation.
Heat-treated leaves of Cassia alata were studied for any change in chemical constituents using sun dried leaves as the reference standard. A high concentration of a constituent was observed in the heat-treated leaves. Spectroscopic studies revealed the structure of the constituent as kaempferol 3-gentiobioside, which has not yet been detected in the Cassia species. In a stability study disappearance of kaempferol 3-gentiobioside was noted in the sun dried leaves while there was little or no change in the kaempferol 3-gentiobioside concentration in the heat-treated leaves when incubated in an aqueous solution, suggesting a possible presence of enzymatic activities in the sun dried leaves. Therefore, heat-treatment may be a good method to stabilize kaempferol 3-gentiobioside in Cassia alata leaves.
Moriyama
H
H
Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Iizuka
T
T
Nagai
M
M
D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D004355:Drug Stability; D005419:Flavonoids; D006358:Hot Temperature; D044949:Kaempferols; D009682:Magnetic Resonance Spectroscopy; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*
D004187:Disaccharides; D005419:Flavonoids; D044949:Kaempferols; C442435:kaempferol 3-gentiobioside; D011794:Quercetin