title: Immunization with gp96 from Listeria monocytogenes-infected mice is due to N-formylated listerial peptides.
abstract: N-Formylated (N-f-met) peptides derived from proteins of the intracellular bacterium Listeria monocytogenes generate a protective, H2-M3-restricted CD8 T cell response in C57BL/6 mice. N-f-met peptide-specific CTL were generated in vitro when mice previously immunized with gp96 isolated from donor mice infected with L. monocytogenes were stimulated with these peptides. No significant peptide-specific CTL activity was observed in mice immunized with gp96 from uninfected animals. Masses corresponding to one N-f-met peptide were found by matrix-assisted laser desorption/ionization-mass spectrometry on gp96 isolated from C57BL/6 mice infected with L. monocytogenes, but not on gp96 from noninfected mice. Therefore, bacterial N-f-met peptides from intracellular bacteria can bind to gp96 in the infected host, and gp96 loaded with these peptides can generate N-f-met-peptide-specific CTL. We assume a unique role of gp96 in Ag processing through the H2-M3 pathway.
authors.affiliation: Department of Immunology, Max-Planck Institute for Infection Biology, Berlin, Germany.
authors.forename: A M
authors.initials: AM
authors.lastname: Sponaas
authors.forename: U
authors.initials: U
authors.lastname: Zuegel
authors.forename: S
authors.initials: S
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: Hurwitz
authors.forename: R
authors.initials: R
authors.lastname: Winter
authors.forename: S
authors.initials: S
authors.lastname: Lamer
authors.forename: P R
authors.initials: PR
authors.lastname: Jungblut
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D008089:Listeria monocytogenes / Q000276:immunology* / Q000378:metabolism*; D008088:Listeriosis / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009239:N-Formylmethionine / Q000378:metabolism*; D009842:Oligopeptides / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D009928:Organ Specificity / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; D009842:Oligopeptides; C052646:sarcoma glycoprotein gp96 rejection antigens; D009239:N-Formylmethionine