Sorting of internalized neurotrophins into an endocytic transcytosis pathway via the Golgi system: Ultrastructural analysis in retinal ganglion cells.
Subcellular pathways and accumulation of internalized radiolabeled neurotrophins NGF, BDNF, and NT-3 were examined in retinal ganglion cells (RGCs) of chick embryos by using quantitative electron microscopic autoradiography. All three neurotrophins accumulated in endosomes and multivesicular bodies. BDNF and NGF also concentrated at the plasma membrane, whereas NT-3 accumulated transiently in the Golgi system. The enhanced targeting of NT-3 to the Golgi system correlated with the anterograde axonal transport of this neurotrophin. Anterograde transport of NT-3, but not its internalization, was significantly attenuated by the tyrosine kinase (trk) inhibitor K252a. Abolishment of trk activity with K252a shifted NT-3 (and BDNF) away from the Golgi system and into a lysosomal pathway, indicating that trk activity regulated sorting of the ligand-receptor complex. Cross-linking of neurotrophins and immunoprecipitation with antibodies to the neurotrophin receptors p75, trkA, trkB, and trkC showed that the large majority of exogenous, receptor-bound NT-3 was bound to trkC in RGC somata, but during anterograde transport in the optic nerve most receptor-bound NT-3 was associated with p75, and after arrival and release in the optic tectum transferred to presumably postsynaptic trkC. These results reveal remarkable and unexpected differences in the intracellular pathways and fates of different neurotrophins within the same cell type. They provide first evidence for an endocytic pathway of internalized neurotrophic factors via the Golgi system before anterograde transport and transcytosis. The results challenge the belief that after internalization all neurotrophins are rapidly degraded in lysosomes.
Butowt
R
R
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada 89557, USA.
von Bartheld
C S
CS
D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002642:Chick Embryo; D003574:Cytochrome c Group / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D019208:Brain-Derived Neurotrophic Factor; D003574:Cytochrome c Group; D004791:Enzyme Inhibitors; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor; D011505:Protein-Tyrosine Kinases