Population dynamics of transgenic microorganisms in the different microecosystem conditions.
The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap (r) Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km (r) Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. Grant numbers: 99-04-96017, 25, 00-07-9011.
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Institute of Biophysics, Siberian Branch, Russian Academy of Sciences Akademgorodok, Krasnoyarsk, 660036, Russia. lubg@ibp.krasnoyarsk.su
Lobova
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Krylova
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Kargatova
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Maksimova
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Boyandin
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Pechurkin
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D000222:Adaptation, Physiological; D001412:Bacillus subtilis; D003264:Containment of Biohazards; D017753:Ecosystem; D004926:Escherichia coli; D005618:Fresh Water / Q000382:microbiology; D015870:Gene Expression; D008837:Micrococcus; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D009997:Osmotic Pressure; D010957:Plasmids; D011157:Population Dynamics; D018570:Risk Assessment; D014871:Water Microbiology