Quantitative determination of naphthoquinones of Impatiens species.
A convenient and reliable reversed phase-HPLC method has been developed and validated for the quantitative determination of naphthoquinones present in the aerial parts of Impatiens glandulifera (Balsaminaceae) during two growing seasons (May to August 1998 and June to October 1999). Maximal content (0.8-1.1%) of 2-hydroxy-1,4-naphthoquinone and its 2-methylated derivative was observed in flowers collected between July and August. The procedure was applied to compare the pigment content in three other species of Impatiens but showed 2.5-37 times lower levels than those found in I. glandulifera.
Lobstein
A
A
Department of Pharmacognosy, Faculty of Pharmacy of Strasbourg, University Louis Pasteur, BP 24, 67401 Illkirch, France. lobstein@pharma.u-strasbg.fr
Brenne
X
X
Feist
E
E
Metz
N
N
Weniger
B
B
Anton
R
R
D029068:Balsaminaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009285:Naphthoquinones / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D009285:Naphthoquinones