title: Spatial temperature gradient capillary electrophoresis for DNA mutation detection.
abstract: A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.
authors.affiliation: Department of Chemistry National University of Singapore, Republic of Singapore.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
authors.forename: B
authors.initials: B
authors.lastname: Lin
authors.forename: E S
authors.initials: ES
authors.lastname: Yeung
mesh_terms: D004247:DNA / Q000032:analysis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D017354:Point Mutation; D013696:Temperature; D013816:Thermodynamics
chemical_list: D007202:Indicators and Reagents; D009692:Nucleic Acid Heteroduplexes; D004247:DNA