Cellular accumulation and retention of the technetium-99m-labelled hypoxia markers BRU59-21 and butylene amine oxime.
BRU59-21 and 99mTc-butylene amine oxime (BnAO, HL91) are being evaluated for imaging hypoxia in tumors. Both tracers: 1) rapidly reached a plateau in aerobic Chinese hamster ovary cells in vitro but continuously accumulated in hypoxic cells; 2) ceased to accumulate when hypoxic cells were exposed to air; 3) showed approximately 40% retention upon washing the cells; 4) showed selective hypoxic accumulation only at 37 degrees C; 5) accumulation could be modulated by addition of electron-affinic compounds; and 6) exhibited higher accumulation in cells which overexpress cytochrome P450 reductase. Both BRU59-21 and 99mTc-BnAO share properties making them suitable for hypoxia imaging.
Zhang
X
X
Division of Experimental Therapeutics, Ontario Cancer Institute, Toronto, Ontario, Canada.
Melo
T
T
Rauth
A M
AM
Ballinger
J R
JR
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D000860:Hypoxia / Q000000981:diagnostic imaging / Q000378:metabolism*; D007093:Imidazoles / Q000378:metabolism*; D015609:Organotechnetium Compounds / Q000378:metabolism*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000378:metabolism*
C403973:BRU 59-21; D007093:Imidazoles; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals