[Studies on synthesis of glutathione by E. coli BL21 (pTrc-gsh) coupled with Saccharomyces cerevisiae].
During synthesis of GSH by the engineered strain E. coli BL21(pTrc-gsh) coupled with Saccharomyces cerevisiae producing ATP from adenosin, the inconsistency of two systems in the concentration of phosphate buffer was solved by decreasing concentration to 250 mmol/L. The conditions under 250 mmol/L phosphate buffer were optimized and the yield of GSH was 1.6 g/L, which was higher than that of summation by two systems under the same conditions respectively. Addition of glycine later after glutamate and cysteine weakened the inhibition of GSH to GSHI. It made the yield of GSH reach to 2.13 g/L which was 30.7% higher than the control.
Shen
L X
LX
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, ECUST, Shanghai 200237, China.
Wei
D Z
DZ
Zhang
S L
SL
Wang
E L
EL
D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002463:Cell Membrane Permeability; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000096:biosynthesis*; D008274:Magnesium / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000255:Adenosine Triphosphate; D005978:Glutathione; D008274:Magnesium; D005947:Glucose; D000241:Adenosine