The conserved methionine residue of the metzincins: a site-directed mutagenesis study.
The metalloprotease clan of the metzincins derive their name from the presence of a conserved methionine residue that is located on the C-terminal side of the zinc-binding consensus sequence HEXXHXXGXXH. This methionine residue is located in a rather divergent part of the primary sequence but is structurally very well conserved. It is located under the pyramidal base of the three histidine residues that coordinate the catalytic zinc ion and is not involved in any direct contact with the metal nor the substrate. In order to clarify its role, this methionine residue (M226) of the protease C from Erwinia chrysanthemi has been mutated to various other amino acids. The mutants M226L, M226A, M226I were sufficiently stable to be isolated, while the mutants M226H, M226S and M226N could not be purified. The kinetic properties of these mutants were analysed. All mutants showed decreased activity, whereby increases in K(M) as well as decreases in k(cat) were observed. The M226L mutant and M226C-E189 K double mutant, which has the catalytic glutamic acid substituted as well, could be crystallised. The structure of the M226L mutant was determined to a resolution of 2.0 A and refined to R(free) of 0.20. The structure is isomorphous to the wild-type and does not show large differences, with the exception of a very small movement of the zinc-liganding histidine residues. The M226C-E189 K double mutant crystal structure has been refined to an R(free) of 0.20 at 2.1 A resolution. A small rearrangement of the zinc-liganding histidine residues can be detected, which leads to a slightly different zinc coordination and could explain the decrease in activity.
Hege
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T
Department of Chemistry and Biochemistry, University of Berne, Berne, CH-3012, Switzerland.
Baumann
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U
D019943:Amino Acid Substitution / Q000235:genetics; D001665:Binding Sites; D016384:Consensus Sequence / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics*; D003460:Crystallization; D018360:Crystallography, X-Ray; D016974:Dickeya chrysanthemi / Q000201:enzymology* / Q000235:genetics; D004795:Enzyme Stability; D018698:Glutamic Acid / Q000235:genetics / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008715:Methionine / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D008024:Ligands; D018698:Glutamic Acid; D006639:Histidine; D008715:Methionine; D012697:Serine Endopeptidases; C088701:protease C; D015032:Zinc