Novel HLA-B alleles identified in potential marrow donors: B*3917, B*1405 and B*3528.
Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing of volunteer bone marrow donors suggested the presence of variants of known HLA-B alleles in five individuals. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Three African-American individuals had a novel HLA-B*39 allele (B*3917), and another African-American was found to have a novel HLA-B*14 allele (B*1405). In a third individual of Hispanic origin, a novel HLA-B*35 allele (B*3528) was identified. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
Gourley
I S
IS
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19035, USA.
Kearns
J
J
Hou
J
J
Wu
J
J
Bassinger
S
S
Montoya
G D
GD
McKeen
M
M
Birkos
S
S
Williams
T M
TM
Kamoun
M
M
D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008913:Minority Groups; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D014019:Tissue Donors
D015235:HLA-B Antigens