[Saikosaponin-d up-regulates GR mRNA expression and induces apoptosis in HL-60 cells].
OBJECTIVE
To research the effect of saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and induction of apoptosis in HL-60 cells.


METHODS
Antiproliferation effects of SSd on HL-60 cells were determined by 3H-thymidine incorporation. Apoptosis of the SSd-treated cells was determined by DNA fragmentation analysis and flow cytometry. GR mRNA was analyzed by Northern blot analysis.


RESULTS
After 48 h treatment with 10 micrograms/ml SSd, 3H-thymidine incorporation was decreased in HL-60 cells and the effects were time and dose dependent. DNA ladder appeared after 60 h treatment with SSd. The flow cytometry analysis showed HL-60 cells was arrested at G0/G1 phase and apoptotic peak appeared in sub-G1 phase. The expression of GR mRNA was increased.


CONCLUSIONS
SSd up-regulates the expression of GR mRNA, inhibits the cell growth and induces apoptosis in HL-60 cells.
Bu
S
S
Department of Physiology, Second Military Medical University, Shanghai 200433.
Xu
J
J
Sun
J
J
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle; D018922:HL-60 Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins; D015854:Up-Regulation
D000972:Antineoplastic Agents, Phytogenic; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D