Induction of islet transplantation tolerance with anti-CD4, anti-CD8 immunotoxins and donor soluble antigen.
OBJECTIVE
To induce islet grafting tolerance by intravenous injection of anti-CD4, anti-CD8 immunotoxins and donor soluble antigen.


METHODS
Fourteen days or 7 days prior to transplantation, the immunotoxin of anti-CD4, anti-CD8 200 micrograms respectively, and donor soluble antigen 500 micrograms were intravenously injected and then 500 donor islets were transplanted under the left renal subcapsular space of diabetes recipients (Sprague-Dawley rats).


RESULTS
The islet grafting survival time for those recipients pretreated with immunotoxin and donor soluble antigen was > 60 days (P < 0.01). The immunotoxins, donor soluble antigen treatment alone might only slightly prolong the grafting survival time.


CONCLUSIONS
The anti-CD4, anti-CD8 immunotoxins jointly used with donor soluble antigen can induce donor specific immunotolerance.
Lan
P
P
Department of Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Yan
L
L
Xiao
L
L
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D006085:Graft Survival / Q000187:drug effects*; D000922:Immunotoxins / Q000494:pharmacology*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015978:Trichosanthin / Q000494:pharmacology
D000906:Antibodies; D015704:CD4 Antigens; D016827:CD8 Antigens; D000922:Immunotoxins; D015978:Trichosanthin