Preservation of canine and feline corneoscleral tissue in Optisol GS.
OBJECTIVE
The objective of the research was to determine whether preservation of corneal tissue of dogs and cats in Optisol GS (OGS, Bausch & Lomb Surgical, Irvine, CA, USA) is feasible for subsequent use in penetrating keratoplasty.


METHODS
The study subjects were 33 dogs and 31 cats with no gross corneal pathology, which had been euthanised by pentobarbital overdose for reasons unrelated to this project.


METHODS
One cornea of each pair was evaluated immediately and the other was evaluated after storage in Optisol GS for either 5, 10, 15 or 20 days. The most important criterion was the preservation of the endothelial cell layer.


RESULTS
Corneoscleral tissue of cats survived longer, when preserved in Optisol GS at 4 degrees C, than that of dogs. Light and scanning electron microscopy revealed good preservation of the endothelial cell layer for up to 10 days in dogs and up to 15 days in cats.
Arndt
C
C
Department of Small Animal Surgery, Veterinary Faculty of the Ludwig-Maximilians-University, Veterinärstr. 13, 80539 Munich, Germany. Carmen_Arndt@gmx.de
Reese
S
S
Köstlin
R
R
D000818:Animals; D002415:Cats; D002809:Chondroitin Sulfates; D045424:Complex Mixtures; D003315:Cornea / Q000502:physiology*; D016895:Culture Media, Serum-Free; D003911:Dextrans; D004285:Dogs; D004728:Endothelium, Corneal / Q000502:physiology / Q000648:ultrastructure*; D005839:Gentamicins; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D009926:Organ Preservation / Q000379:methods / Q000662:veterinary*; D013696:Temperature; D013997:Time Factors
D045424:Complex Mixtures; D016895:Culture Media, Serum-Free; D003911:Dextrans; D005839:Gentamicins; D002809:Chondroitin Sulfates; C075736:Optisol