[Studies on hematopoietic reconstitution by ex vivo expanded human cord blood hematopoietic stem/progenitor cells in SCID mice].
OBJECTIVE
To elucidate the effect of the combination of cytokines on ex vivo expansion and self-renewal potential of human umbilical cord blood hematopoietic stem/progenitor cells.


METHODS
CD34+ cells from cord blood were expanded for 14 days in the culture containing FL + Tpo + SCF + IL-6 + IL-3 + G-CSF and the expanded cells were transplanted into sublethally irradiated SCID mice.


RESULTS
The expanded cells were engrafted smoothly in the SCID recipients and reconstituted their hematopoiesis. Furthermore, human hematopoietic cells could be detected in the marrow of the recipients 6 weeks after transplantation.


CONCLUSIONS
It is possible to expand hematopoietic cells ex vivo efficiently and maintain concomitantly their self-renewal and hematopoietic reconstitution capacities by the combination of FL + Tpo + SCF + IL-6 + IL-3 + G-CSF.
Liu
Y
Y
General Hospital of PLA, Beijing 100853.
Pei
X
X
Lu
S
S
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology; D051379:Mice; D016513:Mice, SCID; D013926:Thrombopoietin / Q000494:pharmacology
D018952:Antigens, CD34; D008565:Membrane Proteins; C084881:flt3 ligand protein; D013926:Thrombopoietin