title: [Retroviral-mediated transcriptional regulatory elements of alpha-fetoprotein gene directed specific expression of interleukin-2 in murine hepatoma].
abstract: OBJECTIVE
  To target expression of interleukin-2 in alpha-fetoprotein (AFP) positive hepatocellular carcinoma.
  
  
  METHODS
  A retroviral vector pDOR-mAFP-IL2 was constructed by coupling the transcriptional regulatory elements (TREs) of murine AFP gene with interleukin-2 cDNA in a retroviral vector.
  
  
  RESULTS
  Subsequent to retroviral infection, expression of IL-2 could be detected in an AFP positive murine hepatoma cell line Hepa1-6 but not in psi 2, an AFP negative murine fibroblast cell line.
  
  
  CONCLUSIONS
  Our results indicate that specific expression of IL-2 in hepatoma cell can be achieved by using of AFP TREs to control the expression of IL-2, and the retroviral vector which we have constructed may be used for further experimental studies on in vivo gene therapy.
authors.affiliation: Cancer Institute, CAMS and PUMC, Beijing 100021.
authors.forename: Q
authors.initials: Q
authors.lastname: Ma
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005809:Genes, Regulator; D015316:Genetic Therapy; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D007376:Interleukin-2; D000509:alpha-Fetoproteins