title: Gelsolin overexpression enhances neurite outgrowth in PC12 cells.
abstract: The rational design of therapies for treating nerve injuries requires an understanding of the mechanisms underlying neurite extension. Neurite motility is driven by actin polymerization; however, the mechanisms are not clearly understood. One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is unclear. We report a two-fold upregulation of gelsolin upon differentiation with nerve growth factor. Cells that were genetically modified to overexpress gelsolin have longer neurites and a greater neurite motility rate compared to controls. These data suggest that gelsolin plays an important role in neurite outgrowth.
authors.affiliation: Department of Chemical Engineering, University of Texas at Austin, 78712, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Furnish
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: C C
authors.initials: CC
authors.lastname: Cunningham
authors.forename: J A
authors.initials: JA
authors.lastname: Kas
authors.forename: C E
authors.initials: CE
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D003001:Cloning, Molecular; D018260:Gelsolin / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video; D009068:Movement / Q000187:drug effects; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018260:Gelsolin; D012333:RNA, Messenger; D020932:Nerve Growth Factor