title: Induction of bone loss by bile duct ligation in rats.
abstract: Metabolic bone loss is the most common complication of chronic liver disease. However, there is little information available about bone loss in rats with a ligated bile duct, a biliary-type of experimental cirrhosis model. Therefore, in this study, the effect of bile duct ligation (BDL) on bone mineral density (BMD) and plasma insulin-like growth factor-I (IGF-I) levels was examined in rats. Two weeks after BDL operation, the rats with a ligated bile duct showed a pronounced and significant decrease in both trabecular and cortical BMD of the femur. In these rats, decreases in food intake and plasma IGF-I levels plus elevated levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and bilirubin were also observed. When the effect of dietary restriction (30% or 50%) over 2 weeks was then investigated in normal rats, 50% food restriction resulted in a significant decrease in femoral trabecular bone density although BMD was unchanged by 30% dietary restriction. The plasma levels of IGF-I were also decreased in food-restricted rats. Thus, the decrease in femoral bone density in 50% food-restricted rats was less potent compared with BDL rats; nevertheless, the decrease in plasma levels of IGF-I was almost equally potent in both BDL and food-restricted rats. Overall, this study showed that BDL operation resulted in a pronounced bone loss in rats, but also that this bone loss might not be merely due to the decreases in food intake and plasma IGF-I levels. These results suggest that BDL in rats is a useful experimental cirrhosis model for evaluating the bone loss associated with chronic liver disease.
authors.affiliation: Department of Metabolic Diseases, Medicinal Biology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Osaka 532-8514, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Mabuchi
authors.forename: I
authors.initials: I
authors.lastname: Kawamura
authors.forename: M
authors.initials: M
authors.lastname: Fushimi
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: S
authors.initials: S
authors.lastname: Takeshita
authors.forename: S
authors.initials: S
authors.lastname: Takakura
authors.forename: M
authors.initials: M
authors.lastname: Matsuo
authors.forename: M
authors.initials: M
authors.lastname: Tomoi
authors.forename: T
authors.initials: T
authors.lastname: Goto
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000503:physiopathology* / Q000601:surgery; D015519:Bone Density; D001862:Bone Resorption / Q000097:blood / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D005269:Femur / Q000473:pathology; D005508:Food Deprivation; D006130:Growth Disorders / Q000097:blood / Q000209:etiology; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D008026:Ligation; D008106:Liver Cirrhosis, Experimental / Q000097:blood / Q000150:complications* / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain
chemical_list: D007334:Insulin-Like Growth Factor I