In-cell NMR spectroscopy.
The recent development of "in-cell NMR" techniques by two independent groups has demonstrated that NMR spectroscopy can be used to characterize the conformation and dynamics of biological macromolecules inside living cells. In this article, we describe different methods and discuss current and future applications as well as critical parameters of this new technique. We show experimental results, compare them with traditional in vitro experiments, and demonstrate that differences between the in vitro and the in vivo state of a macromolecule exist and can be detected and characterized.
Serber
Z
Z
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California 94143, USA.
Dötsch
V
V
D000596:Amino Acids / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000378:metabolism*
D000596:Amino Acids; D011506:Proteins