title: Mass spectrometric methods for the characterisation and differentiation of isomeric O-diglycosyl flavonoids.
abstract: Tandem mass spectrometric methods have been evaluated for the characterisation of the type and the differentiation of the interglycosidic linkage of isomeric flavonoid O-diglycosides. Based on the occurrence of internal monosaccharide residue loss and the relative abundances of Y-type ions formed by fragmentation at glycosidic bonds, four pairs of isomeric flavonoid O-diglycosides can be unambiguously differentiated. The different techniques used, i.e. linked scanning at constant B/E without collisional activation and low-energy collision-induced dissociation using methane or helium as collision gas, have been shown to be useful for distinguishing the two most common (1, 2- and 1, 6-) interglycosidic linkages, e.g. flavonoid O-neohesperidosides and O-rutinosides.
authors.affiliation: University of Antwerp (UIA), Department of Pharmaceutical Sciences, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Y L
authors.initials: YL
authors.lastname: Ma
authors.forename: F
authors.initials: F
authors.lastname: Cuyckens
authors.forename: H
authors.initials: H
authors.lastname: Van den Heuvel
authors.forename: M
authors.initials: M
authors.lastname: Claeys
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005419:Flavonoids / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids; D006027:Glycosides