[Expression of heat shock proteins in laryngeal carcinoma].
OBJECTIVE
To determine the expression of HSPs(HSP90 alpha, HSP90 beta, HSP70, HSP27) in laryngeal carcinoma and normal laryngeal mucosa.


METHODS
Total RNA were extracted from 21 laryngeal cancer tissue samples and the normal mucosa of the corresponding larynx. The expression of HSPs mRNA was examined by Slot blot analysis.


RESULTS
1. HSP90 alpha and HSP70 were selectively overexpressed 5 times more in laryngeal carcinoma than in normal mucosa of larynx. 2. HSP90 beta and HSP27 were constitutively expressed at low level in these two groups. There was no significant difference between the levels of expression of HSP90 beta and HSP27 in laryngeal carcinoma and normal controls.


CONCLUSIONS
The results that HSP90 alpha and HSP 70 mRNA were selectively overexpressed in laryngeal carcinoma suggested that HSP90 alpha and HSP70 might play a specific role in the pathogenesis of laryngeal cancer. To clarify the mechanism of HSPs action could help to provide theorical basis for the biological therapy of laryngeal cancer.
Xu
X
X
Cancer Hospital/Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Zhao
M
M
Shi
Y
Y
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007820:Laryngeal Mucosa / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D018841:HSP90 Heat-Shock Proteins; D012333:RNA, Messenger