title: New DR5 sequences: a novel DRB1*11122 allele identified in Paiwan tribe members of Taiwan and a corrected sequence for the DRB1*1201 allele.
abstract: We report herein the identification of a new DRB1 allele using sequence-based typing (SBT). This novel allele, HLA-DRB1*11122, was found in an aboriginal individual (SWP71) from the Paiwan tribe in the southern part of Taiwan. This individual was typed by SBT method as having an HLA genotype of HLA-A*24021/24021, HLA-B*4001/4002, HLA-DRB1*11122/15011, HLA-DRB3*0202, and HLA-DRB5*01011. This new allele differs from DRB1*1112 in the polymorphic exon 2 only at codon 34 (CAA-->CAG; both specify glutamine) and from DRB1*1110 in the exon 2 sequence only at codon 32 (CAT-->TAT; H32T). The most likely candidate allele which is found in the aboriginal populations of Taiwan and which may mutate into this new allele is DRB1*11011. DRB1*11122 allele differs from DRB1*11011 allele in the polymorphic exon 2 at both codon 34 (CAA-->CAG) and codon 37 (TAC-->TTC; T37F). This novel HLA-DRB1*11122 allele was also found in another aboriginal individual (SWP90) from the same Paiwan tribe. This SWP90 individual was typed by SBT method as having an HLA genotype of HLA-A*24021/24021, HLA-B*4002/5502, HLA-DRB1*11122/1201, and HLA-DRB3*01011/0202. However, the original DRB1*1201 sequence from HERLUFF was found to be erroneously reported and the corrected sequence from SWP90 is now presented herein.
authors.affiliation: Graduate Institute of Human Genetics/Department of Medical Technology, Tzu Chi University, #701, Chung Yang Rd. Section III, Hualien, Taiwan, Republic of China. shaokee@mail.tcu.edu.tw
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: Y M
authors.initials: YM
authors.lastname: Shiao
authors.forename: S M
authors.initials: SM
authors.lastname: Lai
authors.forename: C Y
authors.initials: CY
authors.lastname: Lai
authors.forename: S P
authors.initials: SP
authors.lastname: Chen
authors.forename: Y F
authors.initials: YF
authors.lastname: Chen
authors.forename: D A
authors.initials: DA
authors.lastname: Sidebottom
authors.forename: W H
authors.initials: WH
authors.lastname: Hildebrand
authors.forename: F C
authors.initials: FC
authors.lastname: Chou
authors.forename: M F
authors.initials: MF
authors.lastname: Tsai
mesh_terms: D000483:Alleles; D001483:Base Sequence; D005091:Exons; D005260:Female; D005802:Genes, MHC Class II; D005838:Genotype; D015234:HLA-A Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D015805:HLA-DR5 Antigen / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D044469:Racial Groups; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000208:ethnology
chemical_list: D015234:HLA-A Antigens; D006684:HLA-DR Antigens; D015805:HLA-DR5 Antigen; D059811:HLA-DRB1 Chains; C553518:HLA-DRB1*11:01:01 antigen; C553515:HLA-DRB1*12:12 antigen