title: Mildly oxidized glyceraldehyde-3-phosphate dehydrogenase as a possible regulator of glycolysis.
abstract: Influence of H2O2 on glycolysis was investigated. A hypothesis previously formulated was tested according to which a mild oxidation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) results in uncoupling of oxidation and phosphorylation at this step of glycolysis due to acylphosphatase activity of the oxidized enzyme. Incubation of a mixture of purified glycolytic enzymes, as well as a muscle extract, in the presence of 10-100 microM H2O2 was shown to result in an increase in the rate of glycolysis. The level of lactate accumulation in the oxidized samples increased by 80-150% compared to the samples containing mercaptoethanol. No ATP was formed by the H2O2-stimulated glycolysis. Thus, H2O2 really caused uncoupling of oxidation and phosphorylation in glycolysis. A role of GAPDH oxidation in regulation of glycolysis is discussed.
authors.affiliation: A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Danshina
authors.forename: E V
authors.initials: EV
authors.lastname: Schmalhausen
authors.forename: A V
authors.initials: AV
authors.lastname: Avetisyan
authors.forename: V I
authors.initials: VI
authors.lastname: Muronetz
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010766:Phosphorylation; D011817:Rabbits; D051381:Rats
chemical_list: D019344:Lactic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases