title: Idiopathic membranous glomerulonephritis is associated with altered patterns of self-reactive IgM and IgG antibody repertoires.
abstract: Idiopathic membranous glomerulonephritis (MGN) is an immune complex nephropathy characterized by the subepithelial deposition of immunoglobulin (Ig)G. The pathogenesis of the disease remains largely unknown, but recent evidence suggests that human MGN may involve an autoimmune component. In the present study, we have analyzed the IgM and IgG antibody repertoires of patients with MGN towards self- and nonself-antigens using a technique of quantitative immunoblotting on a panel of whole human tissue or solubilized bacterial cell extracts as sources of antigens. Data were compared by means of multiparametric statistical analysis. We demonstrate that the antibody repertoires of self-reactive IgM and IgG in plasma of patients with MGN exhibit significantly altered patterns of reactivity, as compared with those of healthy controls. In contrast, multiparametric statistical analysis does not discriminate the reactivity patterns of IgM and IgG in plasma of patients and healthy controls towards nonself antigens. These observations indicate that a failure in the regulation of physiological self-reactivity is associated with immune complex nephropathy in MGN.
authors.affiliation: INSERM U430 and Université Pierre et Marie Curie, Hôpital Broussais, 96 rue Didot, 75014 Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Stahl
authors.forename: J P
authors.initials: JP
authors.lastname: Venetz
authors.forename: S
authors.initials: S
authors.lastname: Lacroix-Desmazes
authors.forename: E
authors.initials: E
authors.lastname: Rondeau
authors.forename: E
authors.initials: E
authors.lastname: Bonnin
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
authors.forename: P
authors.initials: P
authors.lastname: Ronco
authors.forename: S V
authors.initials: SV
authors.lastname: Kaveri
mesh_terms: D000328:Adult; D000936:Antigen-Antibody Complex / Q000378:metabolism; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens; D016022:Case-Control Studies; D005260:Female; D015433:Glomerulonephritis, Membranous / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D017634:Self Tolerance
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D001324:Autoantigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M