title: FHIT RNA and protein expression in oral squamous cell carcinomas.
abstract: BACKGROUND
  To investigate the possible role of FHIT, a possible tumour suppressor gene, in oral carcinogenesis, we examined 17 oral squamous cell carcinomas (OSCCs) for genetic alterations.
  
  
  METHODS
  Fresh tissue was obtained during surgery, snap-frozen in liquid nitrogen and stored at -70 degrees C. Nested PCR amplification to examine the integrity of FHIT mRNA was performed on the reverse transcribed complementary DNA obtained from the frozen normal and tumour tissue. Immunohistochemistry was done on formal in-fixed paraffin-embedded tissue protein from the same cases using a polyclonal antiserum against the full length Fhit.
  
  
  RESULTS
  Twelve out 17 (71%) OSCCs showed reduced or absent Fhit protein and half of the cases with reduced Fhit protein exhibited aberrant RT-PCR products.
  
  
  CONCLUSIONS
  Immunohistochemical detection of Fhit protein expression in OSCCs is the more sensitive method to determine the status of Fhit in these tumours, in agreement with previous studies of other tumour types.
authors.affiliation: Department of Oral Pathology and Oral Biology, University of Pretoria, South Africa.
authors.forename: W F
authors.initials: WF
authors.lastname: van Heerden
authors.forename: T J
authors.initials: TJ
authors.lastname: Swart
authors.forename: B
authors.initials: B
authors.lastname: Robson
authors.forename: T L
authors.initials: TL
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Engelbrecht
authors.forename: M B
authors.initials: MB
authors.lastname: van Heerden
authors.forename: E J
authors.initials: EJ
authors.lastname: van Rensburg
authors.forename: K
authors.initials: K
authors.lastname: Huebner
mesh_terms: D017766:Acid Anhydride Hydrolases; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000099170:Bis(5'-adenosyl)triphosphatase