title: Ammocidin, a new apoptosis inducer in Ras-dependent cells from Saccharothrix sp. I. Production, isolation and biological activity.
abstract: A new apoptosis inducer, ammocidin, was isolated from the culture broth of Saccharothrix sp. AJ9571. Ammocidin induced apoptotic cell death in Ras-dependent Ba/F3-V12 cells with an IC50 of 66 ng/ml. No cell death was observed in IL-3-dependent Ba/F3-V12 cells at less than 100 microg/ml of ammocidin. Ammocidin significantly reduced the phosphorylation level of MAPK and S6K that mediate the anti-apoptotic function of Ras.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Tomikawa
authors.forename: K
authors.initials: K
authors.lastname: Shin-ya
authors.forename: J
authors.initials: J
authors.lastname: Shinozaki
authors.forename: T
authors.initials: T
authors.lastname: Kajiura
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
authors.forename: H
authors.initials: H
authors.lastname: Seto
authors.forename: Y
authors.initials: Y
authors.lastname: Hayakawa
mesh_terms: D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D018631:ras Proteins / Q000378:metabolism
chemical_list: D018942:Macrolides; C440627:ammocidin; D018631:ras Proteins