P34(cdc2) kinase is associated with cortical microtubules from higher plant protoplasts.
The cell cycle regulatory enzyme p34(cdc2) kinase is known to be localized to the preprophase band, the spindle and the phragmoplast, but not to interphase cortical microtubules. This was investigated further by mechanically cleaving substrate-attached protoplasts to leave plasma membrane disks bearing microtubules freed of nuclear and cytosolic signal. Antibodies to PSTAIRE and to specific C-terminal peptides of cdc2a, were used in immunofluorescence, protein blotting and immunogold electron microscopy to demonstrate that antigen is located on the cortical microtubules of carrot, tobacco BY-2 and Arabidopsis cells.
Hemsley
R
R
Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich NR4 7UH, UK.
McCutcheon
S
S
Doonan
J
J
Lloyd
C
C
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D018552:Daucus carota / Q000166:cytology / Q000201:enzymology; D004136:Dinitrobenzenes / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D007150:Immunohistochemistry; D008870:Microtubules / Q000201:enzymology* / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism; D059828:Plant Cells; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000201:enzymology; D011523:Protoplasts / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D013424:Sulfanilamides; D014026:Nicotiana / Q000166:cytology / Q000201:enzymology
D000972:Antineoplastic Agents, Phytogenic; D004136:Dinitrobenzenes; D006540:Herbicides; D010446:Peptide Fragments; D010940:Plant Proteins; D013424:Sulfanilamides; C062418:PSTAIR peptide; C012465:oryzalin; D016203:CDC2 Protein Kinase; D017239:Paclitaxel