title: The ankyrin repeat protein Diego mediates Frizzled-dependent planar polarization.
abstract: During planar polarization of the Drosophila wing epithelium, the homophilic adhesion molecule Flamingo localizes to proximal/distal cell boundaries in response to Frizzled signaling; perturbing Frizzled signaling alters Flamingo distribution, many cell diameters distant, by a mechanism that is not well understood. This work identifies a tissue polarity gene, diego, that comprises six ankyrin repeats and colocalizes with Flamingo at proximal/distal boundaries. Diego is specifically required for polarized accumulation of Flamingo and drives ectopic clustering of Flamingo when overexpressed. Our data suggest that Frizzled acts through Diego to promote local clustering of Flamingo, and that clustering of Diego and Flamingo in one cell nonautonomously propagates to others.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Feiguin
authors.forename: M
authors.initials: M
authors.lastname: Hannus
authors.forename: M
authors.initials: M
authors.lastname: Mlodzik
authors.forename: S
authors.initials: S
authors.lastname: Eaton
mesh_terms: D000818:Animals; D017089:Ankyrin Repeat / Q000502:physiology*; D017487:Ankyrins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D019521:Body Patterning / Q000502:physiology; D015820:Cadherins / Q000032:analysis / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000032:analysis / Q000378:metabolism*; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005123:Eye / Q000166:cytology / Q000196:embryology; D051157:Frizzled Receptors; D018507:Gene Expression Regulation, Developmental; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000502:physiology; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction / Q000502:physiology; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology
chemical_list: D017487:Ankyrins; D015820:Cadherins; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D008565:Membrane Proteins; D043562:Receptors, G-Protein-Coupled; C058784:fz protein, Drosophila; C121420:stan protein, Drosophila