title: Adaptation of an [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish.
abstract: OBJECTIVE
  Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish.
  
  
  RESULTS
  The optimal conditions for the colorimetric assay were determined and this method was compared with the trypan blue exclusion assay. The protein concentration of extracellular products causing the death of 50% of the cell population (CI50) was determined.
  
  
  CONCLUSIONS
  This assay enables quantitative and objective comparison of the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. It was shown to be more accurate than conventional counting with the trypan blue exclusion assay.
  
  
  CONCLUSIONS
  This method may also be useful for characterizing the cytotoxicity of specific components of extracellular products.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Malaga, Campus de Teatinos, 29071-Malaga, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Zorrilla
authors.forename: M C
authors.initials: MC
authors.lastname: Balebona
authors.forename: M A
authors.initials: MA
authors.lastname: Moriñigo
mesh_terms: D000818:Animals; D001419:Bacteria / Q000472:pathogenicity*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry; D021541:Sea Bream / Q000382:microbiology*; D013778:Tetrazolium Salts / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*; D014343:Trypan Blue
chemical_list: D001426:Bacterial Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D014343:Trypan Blue