title: Effect of natural phenolic acids on DNA oxidation in vitro.
abstract: We examined the antioxidant activity of the following natural phenolic compounds present in food: 3-OH-benzoic acid (3-OH-BA); 4-OH-benzoic acid (4-OH-BA); 2,3-dihydroxybenzoic acid (2,3-diOH-BA); 3,4-dihydroxybenzoic acid (3,4-diOH-BA or protocatechuic acid); ferulic acid; caffeic acid; and 2-coumaric, 3-coumaric and 4-coumaric acids. We measured the inhibitory effect of these compounds on iron-dependent oxidative DNA damage in vitro [incubating herring sperm DNA with Fe(III)/GSH] or using cumene hydroperoxide (CumOOH) as a free-radical generating system; we also studied the interaction of these phenols with Fe(II) or Fe(III) spectrophotometrically. Among the tested compounds, 2,3-diOH-BA, 3,4-diOH-BA and caffeic acid interacted with Fe(II) and showed a potent inhibitory effect on iron-induced oxidative DNA damage. CumOOH-induced DNA oxidation was not modified by these compounds. On the contrary, 2-coumaric, 3-coumaric and 4-coumaric acids did not interact with iron but protected against oxidative DNA damage induced by Fe(III)/GSH and by CumOOH, indicating a direct free-radical scavenging activity of these compounds in both systems. The IC(50)+/-S.E.M. of the three coumaric acids against CumOOH-induced DNA oxidation was 44.2+/-2.0, 54.7+/-2.0 and 33.1+/-1.0 microM, respectively. On the contrary, 3-OH-BA and 4-OH-BA did not have scavenging activity and 3-OH-BA actually enhanced oxidative DNA damage. In conclusion, some natural phenolic acids, commonly present in food, have interesting protective activity against DNA oxidation in vitro and deserve further consideration as effective antioxidants in vivo.
authors.affiliation: Department of Pharmacology, University of Florence, Viale Pieraccini, 6, 50139, Florence, Italy. maura@pharm.unifi.it
authors.forename: M
authors.initials: M
authors.lastname: Lodovici
authors.forename: F
authors.initials: F
authors.lastname: Guglielmi
authors.forename: M
authors.initials: M
authors.lastname: Meoni
authors.forename: P
authors.initials: P
authors.lastname: Dolara
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001565:Benzoates / Q000378:metabolism / Q000494:pharmacology; D002109:Caffeic Acids / Q000378:metabolism / Q000494:pharmacology; D003373:Coumaric Acids / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D005399:Fishes; D016166:Free Radical Scavengers / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D007501:Iron; D008297:Male; D010084:Oxidation-Reduction; D012661:Semen
chemical_list: D000975:Antioxidants; D001565:Benzoates; D002109:Caffeic Acids; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; C004999:ferulic acid; D007501:Iron; C017616:phenolic acid; C040048:caffeic acid