Characterization of the T lymphocyte subsets and lymphoid populations involved in the induction of low-dose oral tolerance to human thyroglobulin.
Using mice deficient in CD8alpha, TCRdelta, CD4, or CD120a, as well as adoptive transfer experiments in wild-type and RAG-1-deficient mice, we characterized the T lymphocyte subsets and lymphoid populations involved in the induction of low-dose oral tolerance to human thyroglobulin (hTg). The oral administration of hTg, but not the intraperitoneal (ip) administration of hTg, generates lymphocytes that can transfer tolerance. Purified CD8alpha+ lymphocytes successfully transfer tolerance, while the depletion of CD8alpha or TCRdelta lymphocytes prevents the transfer of tolerance. Oral tolerance can be induced in CD4-deficient mice and RAG-1-deficient mice reconstituted with cells from CD120a-deficient mice, but not in CD8alpha-, TCRdelta, or CD120a-deficient mice. These findings indicate that CD8alpha and TCRdelta T lymphocytes are necessary for the oral induction and transfer of tolerance to hTg. Additionally, functional Peyer's patches are necessary for the induction of low-dose oral tolerance to hTg.
Gardine
C A
CA
Thyroid Study Unit, University of Chicago, 5841 South Maryland Avenue, Chicago, IL 60637, USA.
Kouki
T
T
DeGroot
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L
D000284:Administration, Oral; D019264:Adoptive Transfer; D000818:Animals; D015551:Autoimmunity; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance / Q000276:immunology*; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal; D051379:Mice; D008808:Mice, Inbred CBA; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013954:Thyroglobulin / Q000008:administration & dosage / Q000276:immunology*
D013954:Thyroglobulin