Enterotoxins of Staphylococcus aureus induce a cytopathic effect in cell lines.
We describe the use of cell cultures for the detection of Staphylococcus aureus enterotoxins. Madin Darby Bovine Kidney (MDBK), Bovine Embryo lung cells (PEB) and Dog Carcinoma Cell line (A-72) cell monolayers were tested. MDBK, A-72 and PEB cell lines proved to be susceptible to the cytotoxic effect induced by staphylococcal enterotoxins. A cytopathic effect was observed in some cases after 2 hours of incubation. The PEB cell line was the most susceptible and may be used as a useful and cheap method for screening enterotoxigenic S. aureus strains.
Normanno
G
G
Department of Health and Animal Welfare--Faculty of Veterinary Medicine--Valenzano (Bari) Italy.
Celano
G
G
Dambrosio
A
A
Lassandro
L
L
Buonavoglia
C
C
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002417:Cattle; D004285:Dogs; D004768:Enterotoxins / Q000276:immunology / Q000494:pharmacology*; D007369:Interferon Inducers / Q000276:immunology / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology; D008168:Lung / Q000166:cytology; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D017186:Titrimetry; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014774:Virulence
D000906:Antibodies; D004768:Enterotoxins; D007369:Interferon Inducers; C049463:enterotoxin E, Staphylococcal; C019368:enterotoxin D, Staphylococcal; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal