[Localization of a FLT3 ligand isoform in human tissues].
OBJECTIVE
To explore the localization of a human FLT3 ligand isoform deleted 139 bp of extracellular region.


METHODS
Localization of the FLT3 ligand isoform in human tissues was studied by using PCR and RT-PCR. The PCR products were identified by DNA sequencing.


RESULTS
FLT3 ligand was expressed in liver, fetal liver, bone marrow, muscle, kidney and peripheral blood cells and in four leukemia cell lines, but not in brain, while the FLT3 ligand isoform only in fetal liver and bone marrow.


CONCLUSIONS
The FLT3 ligand isoform was expressed in hematopoietic cells and might be involved in hematopoiesis regulation.
Li
H
H
State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Cai
Y
Y
Xu
J
J
D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D008565:Membrane Proteins; D020033:Protein Isoforms; C084881:flt3 ligand protein