Analysis of tumor necrosis factor-alpha gene promoter polymorphisms in anorexia nervosa.
Elevated plasma tumor necrosis factor-alpha (TNFalpha) levels and enhanced spontaneous TNFalpha release from peripheral blood mononuclear cells in patients with anorexia nervosa (AN) have been reported. TNFalpha activates the hypothalamic-pituitary-adrenal axis and reduces food intake, which is characteristic of eating disorders. Recently, three novel polymorphisms in the 5'-flanking region of the TNFalpha gene were reported at positions -1031 (T --> C substitution), -863 (C --> A) and -857 (C --> T). Differences in these alleles are reportedly related to altered TNFalpha-transcriptional promoter activity. Therefore, we performed a case-control association analysis to determine whether any of those three polymorphisms in the TNFalpha promoter region were involved in a predisposition to AN. The results of our analysis of a cohort of 79 female Japanese AN sufferers and 127 normal female control subjects provide no support for the hypothesis that -1031T/C, -863 C/A and -857C/T polymorphisms in the TNFalpha gene promoter region influence the susceptibility to AN.
Ando
T
T
Division of Psychosomatic Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, Ichikawa, Chiba, Japan. ando@ncnp-k.go.jp
Ishikawa
T
T
Kawamura
N
N
Karibe
M
M
Oba
M
M
Tatsuta
N
N
Hara
S
S
Takii
M
M
Naruo
T
T
Takei
M
M
Kurokawa
N
N
Nozoe
S
S
Kubo
C
C
Komaki
G
G
D000328:Adult; D017668:Age of Onset; D000856:Anorexia Nervosa / Q000097:blood / Q000235:genetics*; D015992:Body Mass Index; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006801:Humans; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
D014409:Tumor Necrosis Factor-alpha