title: Miniaturization of capillary isoelectric focusing.
abstract: Miniaturization of whole-column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light-emitting diode (LED) was used as the light source for the whole-column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole-column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.
authors.affiliation: Department of Chemistry, University of Waterloo, ON, Canada.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Wu
authors.forename: S K
authors.initials: SK
authors.lastname: Sze
authors.forename: J
authors.initials: J
authors.lastname: Pawliszyn
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D007525:Isoelectric Focusing / Q000295:instrumentation*; D007526:Isoelectric Point; D008027:Light; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D009211:Myoglobin / Q000737:chemistry; D020033:Protein Isoforms / Q000737:chemistry
chemical_list: D009211:Myoglobin; D020033:Protein Isoforms