title: Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene.
abstract: Primers were designed for the detection of Listeria monocytogenes by the polymerase chain reaction oriented to specific sequences of the inlB gene encoding an internalin. At optimized reaction conditions, 100% sensitivity (on a panel of 33 strains of L. monocytogenes) and 100% specificity (on panels of 15 strains of other Listeria spp. and 41 other bacteria), were determined for the inlB-L/R primers. The detection limit of PCR with these primers was 10(4) cfu/ml and was not affected by up to 10(8) cfu/ml of L. innocua.
authors.affiliation: Department of Microbiology and Chemistry--Food Research Institute, Bratislava, Slovakia.
authors.forename: D
authors.initials: D
authors.lastname: Pangallo
authors.forename: E
authors.initials: E
authors.lastname: Kaclíková
authors.forename: T
authors.initials: T
authors.lastname: Kuchta
authors.forename: H
authors.initials: H
authors.lastname: Drahovská
mesh_terms: D001426:Bacterial Proteins; D017931:DNA Primers; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes