title: Selected contribution: Effects of sex and ovariectomy on responses to platelets in porcine femoral veins.
abstract: Estrogen replacement increases risk of venous thrombosis. In this study, we determined responses in vitro to platelets and platelet products in veins from adult male and intact and ovariectomized female pigs. When contracted with prostaglandin F(2alpha), platelets (25,000 platelets/microl) caused relaxation in veins with endothelium. Higher numbers of platelets caused contraction in veins with and without endothelium. In veins without endothelium, contractions were greater in veins from male than in veins from female pigs, and contractions in intact female pig veins were greater than in ovariectomized females pig veins. Platelet products 5-hydroxytryptamine and thromboxane (analog U-46619) caused comparable contractions in all veins; contractions to prostacyclin were less in veins from intact female pigs. ADP caused comparable endothelium-dependent relaxations in all groups. These relaxations were increased by indomethacin in veins from intact males and females; with inhibition of nitric oxide, relaxations were comparable in all groups. These results suggest that venous responses to platelets vary with sex and presence of ovaries in female pigs. These variations reflect differences in type and quantity of substances released from platelets as well as the sensitivity of the smooth muscle to some vasoactive substances. In addition, products of cyclooxygenase may reduce endothelium-dependent relaxations in veins.
authors.affiliation: Department of Surgery, Mayo Clinic and Foundation, 200 First St. SW, Rochester, MN 55905, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Lewis
authors.forename: M P
authors.initials: MP
authors.lastname: Bracamonte
authors.forename: K S
authors.initials: KS
authors.lastname: Rud
authors.forename: V M
authors.initials: VM
authors.lastname: Miller
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005268:Femoral Vein / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000494:pharmacology; D010052:Ovariectomy; D012016:Reference Values; D012701:Serotonin / Q000494:pharmacology; D012727:Sex Characteristics; D013552:Swine; D014661:Vasoconstriction / Q000502:physiology; D014664:Vasodilation / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D004338:Drug Combinations; D004791:Enzyme Inhibitors; D007476:Ionophores; D014665:Vasodilator Agents; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D012701:Serotonin; D000001:Calcimycin