An antifungal exo-alpha-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum.
Trichoderma harzianum secretes alpha-1,3-glucanases when it is grown on polysaccharides, fungal cell walls, or autoclaved mycelium as a carbon source (simulated antagonistic conditions). We have purified and characterized one of these enzymes, named AGN13.1. The enzyme was monomeric and slightly basic. AGN13.1 was an exo-type alpha-1,3-glucanase and showed lytic and antifungal activity against fungal plant pathogens. Northern and Western analyses indicated that AGN13.1 is induced by conditions that simulated antagonism. We propose that AGN13.1 contributes to the antagonistic response of T. harzianum.
Ait-Lahsen
H
H
Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla-CSIC, Seville, Spain.
Soler
A
A
Rey
M
M
de La Cruz
J
J
Monte
E
E
Llobell
A
A
D000898:Antibiosis; D004789:Enzyme Activation; D005658:Fungi / Q000254:growth & development* / Q000472:pathogenicity; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology; D012333:RNA, Messenger / Q000378:metabolism; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development*
D012333:RNA, Messenger; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase