title: Differential tissue expression of epitopes of the tetraspanin CD151 recognised by monoclonal antibodies.
abstract: CD151, a member of the tetraspanin family of cell membrane proteins, is widely expressed in epithelial, endothelial and muscle cells as well as platelets and megakaryocytes. Several monoclonal antibodies recognising CD151 in transfected cells and immunoprecipitating typical bands of 28 and 32 kDa from cell lysates have been produced. Surprisingly, these antibodies show different patterns of staining on tissue sections and on haemopoietic cells. Here we show that these differences are at least in part due to masking of certain epitopes in integrin/CD151 complexes. These data have important implications for the use of monoclonal antibodies in studies of the distribution and function of CD151. Of six monoclonal antibodies from four laboratories, 11B1 was found to be the most reliable for detection of CD151 in different cell and tissue contexts.
authors.affiliation: Division of Haematology, Hanson Centre for Cancer Research, Rundle Mall, Adelaide, SA 5000, Australia.
authors.forename: S M
authors.initials: SM
authors.lastname: Geary
authors.forename: A C
authors.initials: AC
authors.lastname: Cambareri
authors.forename: P M
authors.initials: PM
authors.lastname: Sincock
authors.forename: S
authors.initials: S
authors.lastname: Fitter
authors.forename: L K
authors.initials: LK
authors.lastname: Ashman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D039422:Integrin alpha3; D039503:Integrin alpha6; D016023:Integrins / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D009132:Muscles / Q000378:metabolism; D011233:Precipitin Tests; D060185:Tetraspanin 24; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C554715:CD151 protein, human; C554716:Cd151 protein, mouse; D000939:Epitopes; D039422:Integrin alpha3; D039503:Integrin alpha6; D016023:Integrins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D060185:Tetraspanin 24