title: Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI.
abstract: Acyl-homoserine-L-lactones (AHLs) are diffusible chemical signals that are required for virulence of many Gram-negative bacteria. AHLs are produced by AHL synthases from two substrates, S-adenosyl-L-methionine and acyl-acyl carrier protein. The AHL synthase EsaI, which is homologous to the AHL synthases from other pathogenic bacterial species, has been crystallized in the primitive tetragonal space group P4(3), with unit-cell parameters a = b = 66.40, c = 47.33 A. The structure was solved by multiple-wavelength anomalous diffraction with a novel use of the rhenium anomalous signal. The rhenium-containing structure has been refined to a resolution of 2.5 A and the perrhenate ion binding sites and liganding residues have been identified.
authors.affiliation: Department of Pharmacology, The University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262, USA.
authors.forename: W T
authors.initials: WT
authors.lastname: Watson
authors.forename: F V
authors.initials: FV
authors.lastname: Murphy
authors.forename: T A
authors.initials: TA
authors.lastname: Gould
authors.forename: P
authors.initials: P
authors.lastname: Jambeck
authors.forename: D L
authors.initials: DL
authors.lastname: Val
authors.forename: J E
authors.initials: JE
authors.lastname: Cronan
authors.forename: S
authors.initials: S
authors.lastname: Beck von Bodman
authors.forename: M E
authors.initials: ME
authors.lastname: Churchill
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D020636:Pantoea / Q000201:enzymology*; D011487:Protein Conformation; D012211:Rhenium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C095458:EsaI protein, Erwinia stewartii; D012211:Rhenium