title: Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen.
abstract: The purpose of this study was to evaluate the effects of phosphoric acid (PA) and a proprietary glutaraldehyde-HEMA aqueous solution (Gluma Desensitizer; GD) on dentin collagen. Specimens of demineralized bovine dentin collagen were treated with either 37% or 50% PA for 1 or 5 min. An additional set of specimens was treated with 37% PA for 1 min followed by GD for 1 min. All specimens were washed with distilled water, lyophilized. reduced with standardized NaB3H4, hydrolyzed with 6 M HCl and subjected to amino acid and cross-link analyses. The results demonstrated that the treatment of demineralized dentin with PA under the conditions tested did not significantly alter the collagen cross-links. The GD-treated samples showed reduction of free lysine (Lys) and hydroxylysine (Hyl) residues, as well as a decrease in the levels of collagen reducible cross-links. In addition, unidentified reducible compounds were detected by high-performance liquid chromatography (HPLC) analysis. These compounds may be derived from cross-links formed between GD-derived aldehyde and Lys/Hyl of collagen. The findings indicate that PA treatment does not significantly affect dentin collagen amino acid and cross-link composition, and that GD treatment affects dentin collagen amino acid and cross-link composition.
authors.affiliation: Department of Operative Dentistry, University of North Carolina at Chapel Hill, 27599-7455, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Ritter
authors.forename: E J
authors.initials: EJ
authors.lastname: Swift
authors.forename: M
authors.initials: M
authors.lastname: Yamauchi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000737:chemistry / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D005612:Freeze Drying; D005976:Glutaral / Q000494:pharmacology*; D006868:Hydrolysis; D006901:Hydroxylysine / Q000032:analysis / Q000737:chemistry; D006909:Hydroxyproline / Q000032:analysis / Q000737:chemistry; D008239:Lysine / Q000032:analysis / Q000737:chemistry; D008689:Methacrylates / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010756:Phosphoric Acids / Q000494:pharmacology*; D013223:Statistics as Topic
chemical_list: D000596:Amino Acids; D008689:Methacrylates; D010756:Phosphoric Acids; C111486:gluma desensitizer; D006901:Hydroxylysine; C005044:hydroxyethyl methacrylate; D003094:Collagen; D008239:Lysine; D006909:Hydroxyproline; D005976:Glutaral