Neutral fluorescence probe with strong ratiometric response to surface charge of phospholipid membranes.
We report on dramatic differences in fluorescence spectra of 4'-dimethylamino-3-hydroxyflavone (probe F) studied in phospholipid membranes of different charge (phosphatidyl glycerol, phosphatidylcholine (PC), their mixture and the mixture of PC with a cationic lipid). The effect consists in variations of relative intensities at two well-separated band maxima at 520 and 570 nm belonging to normal (N*) and tautomer (T*) excited states of flavone chromophore. Based on these studies we propose a new approach to measure electrostatic potential at the surface layer of phospholipid membranes, which is based on potential-dependent changes of bilayer hydration and involves very sensitive and convenient ratiometric measurements in fluorescence emission.
Duportail
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Laboratoire de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculté de Pharmacie, Université Louis Pasteur, Illkirch, France.
Klymchenko
A
A
Mely
Y
Y
Demchenko
A
A
D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry / Q000378:metabolism; D002645:Chickens; D004530:Egg Yolk; D004563:Electrochemistry; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D015335:Molecular Probes / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D055672:Static Electricity
D002593:Cetrimonium Compounds; D005419:Flavonoids; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D015335:Molecular Probes; D010715:Phosphatidylglycerols; D010743:Phospholipids; D000077286:Cetrimonium