Gelsolin overexpression enhances neurite outgrowth in PC12 cells.
The rational design of therapies for treating nerve injuries requires an understanding of the mechanisms underlying neurite extension. Neurite motility is driven by actin polymerization; however, the mechanisms are not clearly understood. One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is unclear. We report a two-fold upregulation of gelsolin upon differentiation with nerve growth factor. Cells that were genetically modified to overexpress gelsolin have longer neurites and a greater neurite motility rate compared to controls. These data suggest that gelsolin plays an important role in neurite outgrowth.
Furnish
E J
EJ
Department of Chemical Engineering, University of Texas at Austin, 78712, USA.
Zhou
W
W
Cunningham
C C
CC
Kas
J A
JA
Schmidt
C E
CE
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D003001:Cloning, Molecular; D018260:Gelsolin / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video; D009068:Movement / Q000187:drug effects; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation / Q000187:drug effects
D018260:Gelsolin; D012333:RNA, Messenger; D020932:Nerve Growth Factor