title: Measurement of serum amyloid A in the neonatal foal using a latex agglutination immunoturbidimetric assay: determination of the normal range, variation with age and response to disease.
abstract: This paper describes the use of a latex agglutination assay to measure serum amyloid A (SAA) in the neonatal foal. The normal range and response to clinical disease was determined. This retrospective study evaluated SAA concentrations over the first 3 days postpartum of 226 Thoroughbred foals judged to be clinically healthy. The normal range for each day was determined; levels were found to be significantly highest on Day 2 (Day 1 vs. Day 2 P<0.0001). The 95th percentile for Days 1-3 was 27.1 mg/l. Clinical records of 133 foals, presented as first or second opinion cases, were evaluated. Foals were divided into 4 groups; septicaemia (S), focal infection (FI), failure of passive transfer (FPT) and noninfectious disease (NI). There was a statistically significant difference (P<0.0001) between SAA concentrations of control foals compared to Groups S and FI. There was no statistically significant difference between controls and Groups FPT and NI. When Group NI was compared to Groups S and FI, there was a statistically significant difference (P<0.0001). The authors suggest that SAA determined by this latex agglutination assay might be a helpful aid in the diagnosis of septicaemia and focal infection in neonatal foals; levels >100 mg/l are highly suggestive of infection in young foals.
authors.affiliation: Beaufort Cottage Stables, Newmarket, Suffolk, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Stoneham
authors.forename: L
authors.initials: L
authors.lastname: Palmer
authors.forename: R
authors.initials: R
authors.lastname: Cash
authors.forename: P D
authors.initials: PD
authors.lastname: Rossdale
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D002138:Calibration; D016022:Case-Control Studies; D005490:Focal Infection / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D006734:Horse Diseases / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D006736:Horses / Q000097:blood*; D007112:Immunity, Maternally-Acquired / Q000502:physiology; D007841:Latex Fixation Tests / Q000662:veterinary; D012015:Reference Standards; D012016:Reference Values; D012189:Retrospective Studies; D018805:Sepsis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D000685:Serum Amyloid A Protein / Q000032:analysis*
chemical_list: D000685:Serum Amyloid A Protein