Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) copolymer from sugars by recombinant Ralstonia eutropha harboring the phaC1Ps and the phaGPs genes of Pseudomonas sp. 61-3.
Heterologous expression of the phaGPs and the phaClPs genes encoding 3-hydroxyacyl acyl carrier protein-coenzyme A transacylase and polyhydroxyalkanoate (PHA) synthase from Pseudomonas sp. 61-3, respectively, was performed in the phbCRe negative mutant, Ralstonia eutropha PHB-4. The recombinant strain of the R. eutropha PHB-4 produced PHA copolymers consisting of 3-hydroxybutyrate (3HB) and medium-chain-length 3-hydroxyalkanoate (mcl-3HA) units of 6-12 carbon atoms from sugars. The 3HB fraction in copolymers was very high (95-97 mol%). The PHA content in the recombinant strain could further be increased by the additional introduction of the phbABRe genes from R. eutropha encoding beta-ketothiolase and NADPH-depedent acetoacetyl-coenzyme A reductase. Differential scanning calorimetry analysis of the PHA copolymers produced by the recombinant R. eutropha PHB-4 have indicated that the PHA is a random copolymer of 3HB and mcl-3HA units.
Matsumoto
K
K
Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Nakae
S
S
Taguchi
K
K
Matsusaki
H
H
Seki
M
M
Doi
Y
Y
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D020118:Cupriavidus necator / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances; D010957:Plasmids / Q000235:genetics; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics; D011995:Recombination, Genetic; D000094122:Polyhydroxybutyrates
D000217:Acyltransferases; D001426:Bacterial Proteins; D006885:Hydroxybutyrates; D046911:Macromolecular Substances; D011091:Polyesters; D011108:Polymers; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C114682:3-hydroxyacyl-CoA-acyl carrier protein transferase; C067032:poly(3-hydroxyalkenoate)polymerase