Synaptic connections between trigemino-parabrachial projection neurons and gamma-aminobutyric acid- and glycine-immunoreactive terminals in the rat.
The synaptic connections between gamma-aminobutyric acid (GABA)- and glycine-immunoreactive terminals and neurons projecting to the lateral parabrachial region were examined by a combination of retrograde tracing and immunohistochemical staining in the rat medullary dorsal horn. After injection of horseradish peroxidase (HRP) into the right lateral parabrachial region, HRP retrogradely labeled neurons were observed bilaterally in laminae I, II and III of the medullary dorsal horn with an ipsilateral predominance. GABA- and glycine-like immunoreactive terminals were found in laminae I, II and III. Some of these GABA- and glycine-like immunoreactive terminals were observed chiefly to make symmetric synapses with HRP-labeled neuronal cell bodies and dendritic processes. The present results indicate that neurons in the medullary dorsal horn projecting to the lateral parabrachial region might be modulated by GABAergic and glycinergic inhibitory intrinsic neurons, which might be significantly involved in the regulation of the noxious information transmission.
Wang
D
D
Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China.
Wu
J H
JH
Dong
Y X
YX
Li
Y Q
YQ
D000818:Animals; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D005998:Glycine / Q000378:metabolism*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009433:Neural Inhibition / Q000502:physiology; D009434:Neural Pathways / Q000378:metabolism / Q000648:ultrastructure*; D009619:Nociceptors / Q000378:metabolism / Q000648:ultrastructure; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D011149:Pons / Q000378:metabolism / Q000648:ultrastructure*; D020671:Posterior Horn Cells / Q000378:metabolism / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D013570:Synaptic Membranes / Q000378:metabolism / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014275:Trigeminal Caudal Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D017833:Visceral Afferents / Q000378:metabolism / Q000648:ultrastructure*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase; D005998:Glycine