Histochemical localization of glutathione dependent NBT-reductase in mouse skin.
OBJECTIVE
Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated.


METHODS
The fresh frozen tissue sections (8 m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange.


RESULTS
The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin.


CONCLUSIONS
The activity of the NBT-reductase seems to be dependent on the glutathione contents.
Shukla
Y
Y
Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Post Box 80, M. G. Marg, Lucknow-226 001, India. Yogeshwer_shukla@hotmail.com
D000818:Animals; D002455:Cell Division; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D051379:Mice; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D012867:Skin / Q000201:enzymology
D009580:Nitroblue Tetrazolium; D010088:Oxidoreductases; D005978:Glutathione