Nonculture prediction of Neisseria meningitidis susceptibility to penicillin.
We developed a nonculture method to predict the susceptibility of Neisseria meningitidis to penicillin G. The penA gene was amplified and submitted to restriction fragment length polymorphism analysis. This approach was first validated with a collection of 75 meningococcal strains of known phenotypes. It was next successfully applied to 29 clinical samples.
Antignac
A
A
Unité des Neisseria, Centre National de Référence des Méningocoques, Institut Pasteur, 75724 Paris Cedex 15, France.
Alonso
J M
JM
Taha
M K
MK
D000483:Alleles; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009345:Neisseria meningitidis / Q000187:drug effects* / Q000235:genetics; D010400:Penicillin G / Q000494:pharmacology*; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology*; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004269:DNA, Bacterial; D010406:Penicillins; D010400:Penicillin G