title: Evaluation of accuracy and reliability of indirect calorimetry for the measurement of resting energy expenditure in healthy dogs.
abstract: OBJECTIVE
  To assess accuracy and reliability of open-flow indirect calorimetry in dogs.
  
  
  METHODS
  13 clinically normal dogs.
  
  
  METHODS
  In phase 1, oxygen consumption per kilogram of body weight (VO2/kg) was determined in 6 anesthetized dogs by use of open-flow indirect calorimetry before and after determination of VO2/kg by use of closed-circuit spirometry. In phase 2, four serial measurements of VO2 and carbon dioxide production (VCO2) were obtained in 7 awake dogs by use of indirect calorimetry on 2 consecutive days. Resting energy expenditure (REE) was calculated.
  
  
  RESULTS
  Level of clinical agreement was acceptable between results of indirect calorimetry and spirometry. Mean VO2/kg determined by use of calorimetry before spirometry was significantly greater than that obtained after spirometry. In phase 2, intraclass correlation coefficients (ICC) for REE and VO2 were 0.779 and 0.786, respectively, when data from all 4 series were combined. When the first series was discounted, ICC increased to 0.904 and 0.894 for REE and VO2, respectively. The most reliable and least variable measures of REE and VO2 were obtained when the first 2 series were discounted.
  
  
  CONCLUSIONS
  Open-flow indirect calorimetry may be used clinically to obtain a measure of VO2 and an estimate of REE in dogs. Serial measurements of REE and VO2 in clinically normal dogs are reliable, but a 10-minute adaption period should be allowed, the first series of observations should be discounted, multiple serial measurements should be obtained, and REE.
authors.affiliation: Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: E
authors.initials: E
authors.lastname: O'Toole
authors.forename: W N
authors.initials: WN
authors.lastname: McDonell
authors.forename: B A
authors.initials: BA
authors.lastname: Wilson
authors.forename: K A
authors.initials: KA
authors.lastname: Mathews
authors.forename: C W
authors.initials: CW
authors.lastname: Miller
authors.forename: W C
authors.initials: WC
authors.lastname: Sears
mesh_terms: D000818:Animals; D002153:Calorimetry, Indirect / Q000379:methods / Q000662:veterinary*; D002245:Carbon Dioxide / Q000032:analysis; D004285:Dogs / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D015203:Reproducibility of Results; D013147:Spirometry / Q000662:veterinary; D018709:Statistics, Nonparametric
chemical_list: D002245:Carbon Dioxide