[Cloning, expression and purification of the chicken growth and differentiation factor-8].
Growth and Differentiation Factor-8(GDF-8) is a new member of TGF-beta super-family. It has been shown that GDF-8 is specifically expressed in skeleton muscle in mouse and its function is to inhibit the growth of muscle cell, so it is named as Myostatin. Here, we amplified 3'half-length GDF-8 cDNA from chicken skeleton muscle by RT-PCR, and cloned it into the prokaryotic expression vector pTrcHisB, which was then transformed into E. coli Top10 cells. The recombinant 6 x His-GDF-8 fusion protein expressed in the Top10 cells was purified by Ni(+)-Affinity Chromatography for future study.
Yang
W
W
Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Wang
H X
HX
Chen
Y
Y
Zhang
Y
Y
Zhu
D H
DH
D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018482:Muscle, Skeletal / Q000254:growth & development; D055435:Myostatin; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
C525912:Mstn protein, mouse; D055435:Myostatin; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta