MCF-10A-NeoST: a new cell system for studying cell-ECM and cell-cell interactions in breast cancer.
OBJECTIVE
There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers.


METHODS
We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer.


RESULTS
NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells.


CONCLUSIONS
MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
Zantek
N D
ND
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47905, USA.
Walker-Daniels
J
J
Stewart
J
J
Hansen
R K
RK
Robinson
D
D
Miao
H
H
Wang
B
B
Kung
H J
HJ
Bissell
M J
MJ
Kinch
M S
MS
D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D048429:Cell Size / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013997:Time Factors
D011505:Protein-Tyrosine Kinases