Diminished corneal angiogenesis in gelatinase A-deficient mice.
The goal of the present study was to define the role of gelatinase A in angiogenesis. We performed corneal micropocket assays in gelatinase A-deficient mice and their age-matched wild-type littermates. The corneal neovascular area in gelatinase A-deficient mice (0.15+/-0.14 mm(2)) was significantly less than that of wild-type littermates (0.53+/-0.35 mm(2); P<0.01). Similarly, aortic ring assays showed significant reduction of endothelial outgrowth in gelatinase A-deficient mice (0.26+/-0.14 mm(2)) as compared to wild-type littermates (0.44+/-0.06 mm(2); P<0.05). These results suggest that gelatinase A may play an important role in the regulation of corneal angiogenesis.
Kato
T
T
The Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA.
Kure
T
T
Chang
J H
JH
Gabison
E E
EE
Itoh
T
T
Itohara
S
S
Azar
D T
DT
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003315:Cornea / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D016510:Corneal Neovascularization / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D017353:Gene Deletion; D005838:Genotype; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic / Q000187:drug effects
D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2