title: Bartonella spp antibodies and DNA in aqueous humour of cats.
abstract: Bartonella spp antibodies were measured in the serum and aqueous humour of cats with and without uveitis and polymerase chain reaction (PCR) for Bartonella spp DNA was performed on aqueous humour from most of the cats. Serum and aqueous humour were assayed from 49 client-owned cats with uveitis, 49 healthy shelter cats, and nine cats experimentally inoculated with either B henselae or B clarridgeiae, 454 days after inoculation. An aqueous antibody coefficient (C value) was calculated for cats positive for Bartonella spp antibodies in the aqueous humour. Ocular production of Bartonella spp IgG (C value >1) was detected in seven of 49 cats with uveitis, none of 49 healthy shelter cats, and four of nine experimentally inoculated cats. The organism was detected by PCR in the aqueous humour of three of 24 cats with uveitis, one of 49 healthy shelter cats, and four of nine experimentally inoculated cats. Bartonella spp infect the eyes of some cats following natural exposure or experimental inoculation and may cause uveitis in some cats.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Lappin
authors.forename: D L
authors.initials: DL
authors.lastname: Kordick
authors.forename: E B
authors.initials: EB
authors.lastname: Breitschwerdt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001082:Aqueous Humor / Q000382:microbiology*; D001473:Bartonella / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D002371:Cat Diseases / Q000276:immunology / Q000382:microbiology*; D002415:Cats; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007074:Immunoglobulin G / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014605:Uveitis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial; D007074:Immunoglobulin G