title: [Factor V Leiden and PTG20210A gene mutation in patients with venous thrombosis and healthy blood donors].
abstract: OBJECTIVE
  To evaluate the incidence of factor V Leiden and prothrombin gene G20210A mutation in patients with venous thrombosis and healthy volunteers.
  
  
  METHODS
  Factor V Leiden and prothrombin gene G20210A mutation were analysed in 97 cases of venous thrombosis and 100 healthy volunteers with the methods of one-step PCR-RFLP.
  
  
  RESULTS
  PCR products for the factor V gene (175 bp) and for the prothrombin gene (118 bp) were identified to 157 bp and 98 bp fragments by electrophoresis after Taq I treatment. No factor V Leiden and prothrombin gene G20210A mutation were found in either group.
  
  
  CONCLUSIONS
  The low incidence of factor V Leiden and prothrombin gene G20210A mutation suggest that they are not the major genetic risk factors for thrombophilia in the Chinese.
authors.affiliation: PUMC Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.
authors.forename: C
authors.initials: C
authors.lastname: Bai
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: X
authors.initials: X
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D011516:Prothrombin / Q000235:genetics*; D011655:Pulmonary Embolism / Q000209:etiology; D020246:Venous Thrombosis / Q000150:complications / Q000235:genetics*
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin