Dependence of erythrocyte vesiculation and hemolysis parameters on the concentration of sodium dodecyl sulfate. Vesicular-competitive hemolysis.
The kinetic and concentration dependences of erythrocyte vesiculation and hemolysis induced by sodium dodecyl sulfate were studied. The similarity of the slopes of the dose dependence of the SDS-induced vesiculation and slow hemolysis rates in the double logarithmic coordinates suggested a close relation between the processes of vesiculation and pore formation for slow hemolysis by the detergent. Further evidence of the competitive nature of the detergent-induced vesiculation and fast hemolysis by sodium dodecyl sulfate was obtained. The phenomenon of partial hemolysis proceeding at a rate comparable to that of cell vesiculation is explained in terms of the competition between hemolysis and vesiculation, without resorting to erythrocyte heterogeneity. New vesicular-competitive hemolysis is described. Based on it, the action of different hemolysis-inducing agents is analysed.
Chernitsky
E A
EA
Institute of Photobiology, National Academy of Sciences of Belarus, Minsk, Republic of Belarus. sei@biobel.bas-net.by
Senkovich
O A
OA
Rozin
V V
VV
D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
D003902:Detergents; D012967:Sodium Dodecyl Sulfate