Oligothiophene isothiocyanates as a new class of fluorescent markers for biopolymers.
The regioselective synthesis of fluorescent oligothiophene isothiocyanates is described. The isothiocyanates were reacted with bovine serum albumin (BSA) following standard procedures and the optical properties of the oligothiophene-BSA conjugates were analyzed as a function of oligomer concentration, time, and irradiation power. The oligothiophene-BSA conjugates were chemically very stable and their photoluminescence characteristics persisted unaltered for several months. Photoluminescence data relative to the conjugate of an oligothiophene-S,S-dioxide isothiocyanate with monoclonal anti-CD8 antibody are reported. No fluorescence quenching was observed following the binding of the isothiocyanate to the antibody and the conjugate displayed high chemical stability and photostability.
Barbarella
G
G
Consiglio Nazionale Ricerche, I.Co.C.E.A, Via Gobetti 101, 40129 Bologna, Italy.
Zambianchi
M
M
Pudova
O
O
Paladini
V
V
Ventola
A
A
Cipriani
F
F
Gigli
G
G
Cingolani
R
R
Citro
G
G
D000911:Antibodies, Monoclonal / Q000737:chemistry; D001704:Biopolymers / Q000032:analysis; D016827:CD8 Antigens / Q000276:immunology; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D017879:Isothiocyanates / Q000138:chemical synthesis* / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013876:Thiophenes / Q000138:chemical synthesis* / Q000737:chemistry
D000911:Antibodies, Monoclonal; D001704:Biopolymers; D016827:CD8 Antigens; D005456:Fluorescent Dyes; D018796:Immunoconjugates; D017879:Isothiocyanates; D013876:Thiophenes; D012710:Serum Albumin, Bovine