title: Instability of chromosome 17 and the p53 locus in non-familial colorectal cancer with multiple primary malignancies.
abstract: Recently, two different mechanisms of genetic instability have been demonstrated in the carcinogenesis of colorectal cancer. Microsatellite instability is an important genetic event for carcinogenesis in hereditary non-polyposis colorectal cancer, proximal colon cancer, and multiple colorectal carcinoma. To examine the association among chromosomal instability and multiple primary malignancies (MPM) in colorectal cancer, fluorescence in situ hybridization using a chromosome 17-specific probe, p53 cosmid probe, and/or an alpha satellite DNA probe was performed in 184 patients with colorectal cancer. The proportion of aneusomy 17 in MPM was significantly higher than that of single cancers (SC) (46.1+/-8.0% and 39.0+/-10.3%, respectively; p<0.01). Multiple numerical aberrations of chromosome 17 in MPM occurred more often than those of SC (64.3% and 22.9%, respectively; p<0.01). The mean frequency of p53 deletion was also higher in MPM (70.4+/-16.7%) compared with SC (53.4+/-18.1%, p<0.05). The frequency of chromosome 17 translocation was significantly greater in tumors with MPM (4/6; 67%) than in SC (3/23; 13%, p<0.05). The frequency of p53 locus translocation was also significantly greater in tumors with MPM (4/6; 67%) than in SC (0/23; 0%, p<0.01). These results suggested that numerical and structural aberrations of chromosome 17 and the p53 locus are important genetic events associated with carcinogenesis in non-familial colorectal cancer with MPM.
authors.affiliation: First Department of Surgery, Nagasaki University School of Medicine, Nagasaki-shi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sawai
authors.forename: A
authors.initials: A
authors.lastname: Nanashima
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Yasutake
authors.forename: T
authors.initials: T
authors.lastname: Nakagoe
authors.forename: H
authors.initials: H
authors.lastname: Ayabe
authors.forename: Y
authors.initials: Y
authors.lastname: Tagawa
mesh_terms: D000782:Aneuploidy; D002873:Chromosome Fragility; D002886:Chromosomes, Human, Pair 17; D003110:Colonic Neoplasms / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000235:genetics; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics; D017353:Gene Deletion; D016158:Genes, p53; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009006:Monosomy; D009378:Neoplasms, Multiple Primary / Q000235:genetics*; D012004:Rectal Neoplasms / Q000235:genetics*; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes; D004276:DNA, Satellite