Identification of pestiviruses contaminating cell lines and fetal calf sera.
Three pestiviruses in fetal calf serum (FCS), a pestivirus in porcine ST cell line, and two pestiviruses in two of five bovine cell lines were detected by RT-PCR method employing panpestivirus primers selected from the 5'-non-coding region (5'-NCR). The 288 bp products were sequenced in both directions. To identify these pestiviruses, their nucleotide sequences and those of reference pestiviruses were used for construction of a dendrogram. Three viruses present in FCS and a virus present in the bovine RP-15 cell line were identified as bovine viral diarrhea virus I (BVDV-1). Bovine viral diarrhea virus 2 (BVDV-2) was identified in a batch of the bovine MDBK cell line. A pestivirus contaminating the porcine ST cell line was identified as a Border disease virus (BDV).
Vilcek
S
S
University of Veterinary Medicine, Kosice, Slovak Republic. vilcek@uvm.sk
D000818:Animals; D000833:Animals, Suckling / Q000097:blood / Q000821:virology*; D001483:Base Sequence; D018083:Border disease virus / Q000302:isolation & purification; D002417:Cattle; D002460:Cell Line / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011786:Quality Control; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine