title: Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil.
abstract: Dogs sera samples collected from Cotia County, São Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7%) serum samples were positive in the ELISA; 20 of them (86.9%) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7%) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X(2) = 0.725; p = 0.394) what suggests absence of serological cross reactivity.
authors.affiliation: Divisão Técnica de Medicina Veterinária e Biologia da Fauna, Secretaria Municipal do Meio Ambiente da Prefeitura Municipal de São Paulo, São Paulo, SP, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: Joppert
authors.forename: M K
authors.initials: MK
authors.lastname: Hagiwara
authors.forename: N H
authors.initials: NH
authors.lastname: Yoshinari
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D015153:Blotting, Western; D025065:Borrelia burgdorferi / Q000276:immunology*; D001938:Brazil / Q000453:epidemiology; D016009:Chi-Square Distribution; D004283:Dog Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008193:Lyme Disease / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D016036:Seroepidemiologic Studies
chemical_list: D000907:Antibodies, Bacterial