title: Analysis of tumor necrosis factor-alpha gene promoter polymorphisms in anorexia nervosa.
abstract: Elevated plasma tumor necrosis factor-alpha (TNFalpha) levels and enhanced spontaneous TNFalpha release from peripheral blood mononuclear cells in patients with anorexia nervosa (AN) have been reported. TNFalpha activates the hypothalamic-pituitary-adrenal axis and reduces food intake, which is characteristic of eating disorders. Recently, three novel polymorphisms in the 5'-flanking region of the TNFalpha gene were reported at positions -1031 (T --> C substitution), -863 (C --> A) and -857 (C --> T). Differences in these alleles are reportedly related to altered TNFalpha-transcriptional promoter activity. Therefore, we performed a case-control association analysis to determine whether any of those three polymorphisms in the TNFalpha promoter region were involved in a predisposition to AN. The results of our analysis of a cohort of 79 female Japanese AN sufferers and 127 normal female control subjects provide no support for the hypothesis that -1031T/C, -863 C/A and -857C/T polymorphisms in the TNFalpha gene promoter region influence the susceptibility to AN.
authors.affiliation: Division of Psychosomatic Research, National Institute of Mental Health, National Center of Neurology and Psychiatry, Ichikawa, Chiba, Japan. ando@ncnp-k.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: N
authors.initials: N
authors.lastname: Kawamura
authors.forename: M
authors.initials: M
authors.lastname: Karibe
authors.forename: M
authors.initials: M
authors.lastname: Oba
authors.forename: N
authors.initials: N
authors.lastname: Tatsuta
authors.forename: S
authors.initials: S
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Takii
authors.forename: T
authors.initials: T
authors.lastname: Naruo
authors.forename: M
authors.initials: M
authors.lastname: Takei
authors.forename: N
authors.initials: N
authors.lastname: Kurokawa
authors.forename: S
authors.initials: S
authors.lastname: Nozoe
authors.forename: C
authors.initials: C
authors.lastname: Kubo
authors.forename: G
authors.initials: G
authors.lastname: Komaki
mesh_terms: D000328:Adult; D017668:Age of Onset; D000856:Anorexia Nervosa / Q000097:blood / Q000235:genetics*; D015992:Body Mass Index; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006801:Humans; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012016:Reference Values; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha