Identification of ST2A1 as a rat brain neurosteroid sulfotransferase mRNA.
A hydroxysteroid sulfotransferase (ST2A1) was identified as a form mediating neurosteroid sulfation in rat brain. The sole expression among known rat ST2A forms was indicated by brain RT-PCR. All nucleotide sequences of seven ST2A cDNA clones isolated from brain matched completely with that of hepatic ST2A1. The recombinant ST2A1 protein mediated neurosteroid sulfation. These data strongly suggest a functional role of ST2A1 as a neurosteroid sulfotransferase in rat brain.
Shimada
M
M
Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki Aoba, Aoba-ku, 980-8578, Sendai, Japan. shimada@mail.pharm.tohoku.ac.jp
Yoshinari
K
K
Tanabe
E
E
Shimakawa
E
E
Kobashi
M
M
Nagata
K
K
Yamazoe
Y
Y
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D003345:Corticosterone / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D019314:Dehydroepiandrosterone Sulfate / Q000378:metabolism; D005260:Female; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D010724:Phosphoadenosine Phosphosulfate / Q000494:pharmacology; D011284:Pregnenolone / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015238:Sulfotransferases / Q000032:analysis / Q000096:biosynthesis*
D007527:Isoenzymes; D012333:RNA, Messenger; D010724:Phosphoadenosine Phosphosulfate; D019314:Dehydroepiandrosterone Sulfate; D011284:Pregnenolone; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; D003345:Corticosterone