title: Cloning and characterization of a novel gene encoding keratin-like protein from nematode Nippostrongylus brasiliensis.
abstract: Nippostrongylus brasiliensis (Nb) is one of the most important parasites in studying Th2 immune response of the host, but little is known about its antigenic structures of the excretory-secretory or structural proteins of the parasite. Here we report cloning and characterization of a novel antigenic gene from cDNA library of Nb adult worm by immunoscreening. The positive clone, KLP-Nb, had an open reading frame of 612 bp that encodes a 203-amino-acid protein and was homologous to 'similar to keratins in a glycine-rich region' of Caenorhabditis elegans. Its expression was confirmed by Northern blotting and IgG enzyme-linked immunosorbent assay. This protein seems to be one of the components of cuticle that covers the nematode body.
authors.affiliation: Department ofPediatrics, Shinshu University School of Medicine, Asahi, Matsumoto, Japan. shibui@sch.md.shinshu-u.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Shibui
authors.forename: M
authors.initials: M
authors.lastname: Takamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: A
authors.initials: A
authors.lastname: Komiyama
authors.forename: K
authors.initials: K
authors.lastname: Sugane
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D007633:Keratins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009559:Nippostrongylus / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D016415:Sequence Alignment
chemical_list: D007633:Keratins