[The feature of T cell clonality with polycythemia vera].
OBJECTIVE
To investigate the expression and clonality of TCR V beta subfamily genes in the T cells in polycythemia vera.


METHODS
The 24 genes of the TCR V beta subfamily were amplified with peripheral blood mononuclear cells from 3 cases of polycythemia vera(PV) by RT-PCR, then the PCR products were further analyzed by genescan technique to evaluating the clonality of the detectable TCR V beta T cells.


RESULTS
Only 4-14 of the 24 V beta subfamily T cells were found in the 3 PV cases, the commonly expressed V beta subfamily genes were V beta 2, V beta 3, V beta 16, V beta 21 and V beta 23. Two clonal T cells of V beta 3 and V beta 23 were identified in 2 cases.


CONCLUSIONS
The skew distribution and clonal expansion of TCR V beta subfamily T cells in patients with polycythemia vera might be a feature of the immune response for malignant cells.
Li
Y
Y
Department of Hematology, Jinan University, Guangzhou 510632.
Yang
L
L
Chen
S
S
D003001:Cloning, Molecular; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics; D019674:Genes, T-Cell Receptor beta / Q000235:genetics*; D006801:Humans; D011087:Polycythemia Vera / Q000235:genetics* / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D016693:Receptors, Antigen, T-Cell, alpha-beta