SF20/IL-25, a novel bone marrow stroma-derived growth factor that binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation.
Using a forward genetic approach and phenotype-based complementation screening to search for factors that stimulate cell proliferation, we have isolated a novel secreted bone marrow stroma-derived growth factor, which we termed SF20/IL-25. This protein signals cells to proliferate via its receptor, which we have identified as mouse thymic shared Ag-1 (TSA-1). Enforced expression of TSA-1 in IL-3-dependent Ba/F3 cells that do not express endogenous TSA-1 rendered cells to proliferate in a dose-dependent manner when stimulated with SF20/IL-25. FDCP2, a factor-dependent hemopoietic cell line that expresses endogenous TSA-1, could also be stimulated to proliferate with SF20/IL-25. Binding of SF20 to TSA-1 was blocked by anti-TSA-1 Ab and SF20-induced proliferation of TSA-1-expressing cells was inhibited by anti-TSA-1. In vitro assay revealed that SF20/IL-25 has no detectable myelopoietic activity but supports proliferation of cells in the lymphoid lineage.
Tulin
E E
EE
Chugai Research Institute for Molecular Medicine, Niihari, Ibaraki, Japan. tulin@cimmed.com
Onoda
N
N
Nakata
Y
Y
Maeda
M
M
Hasegawa
M
M
Nomura
H
H
Kitamura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression / Q000276:immunology; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D020381:Interleukin-17; D007378:Interleukins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D017154:Stromal Cells / Q000276:immunology / Q000378:metabolism
D006133:Growth Substances; C486701:IL25 protein, human; D020381:Interleukin-17; D007378:Interleukins; D008565:Membrane Proteins; C442281:Mydgf protein, mouse; C084839:thymic shared antigen-1