Complementation of bipartite begomovirus movement functions by topocuviruses and curtoviruses.
The DNA A components of bipartite species of the genus Begomovirus (family Geminiviridae) encode all viral functions required for replication and encapsidation but require gene products encoded on DNA B for cell-to-cell movement in plants. We demonstrate that geminiviruses of the genera Curtovirus and Topocuvirus are able to trans-complement efficient movement of DNA A for two bipartite begomoviruses in the absence of their corresponding DNA B. It previously has been shown that DNA A can, at low efficiency and without inducing symptoms, spread in the absence of DNA B. The spread of DNA A independent of DNA B, following Agrobacterium-mediated inoculation, is shown to require coat protein whereas trans-complemented spread of DNA A can occur independent of the coat protein encoded on DNA A. The significance of these findings to our understanding of the geminiviral infection cycle is discussed.
Briddon
R W
RW
Department of Virus Research, John Innes Centre, Norwich, UK. rob.briddon@bbsrc.ac.uk
Markham
P G
PG
D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D005816:Genetic Complementation Test; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins; D012231:Rhizobium / Q000235:genetics; D014026:Nicotiana; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D004279:DNA, Viral; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins