Assessment of newly developed perfluorocarbon emulsion: oxygen carrying capacity as the blood substitute in vivo.
This study provides the evaluation of oxygen carrying capacity of the novel perfluorocarbon emulsion (Neo-PFC) produced by the new emulsifying technology named High Pressure Process. For the performance comparison of oxygen carrying abilities of Neo-PFC and a representative PFC emulsion, the oxidation states of cerebral tissues in substituted animals were measured by near-infrared spectrometry. After the 70% exchange transfusion of whole blood of rats by Neo-PFC and Fluosol-DA, fractional inspired oxygen (FiO2) was gradually decreased from 100% to 0%. As the control experiments, the blood was substituted by Krebs Ringer bicarbonate buffer containing 3% BSA. When the blood of rats was substituted by Neo-PFC, Cyt. ox., a terminal enzyme in mitochondrial respiratory chain maintained fully oxidized state with FiO2 values between 100 to 40%. By contrast, in the models substituted by Fluosol-DA and BSA-buffer. Cyt. ox. was gradually reduced with FiO2 values below 60% and 80%, respectively. This specific advantage of Neo-PFC was explained by its higher oxygen solubility in arterial blood. The novel PFC emulsion prepared by the new emulsifying technology is a potential basis for blood substitutes.
Sakanoue
J
J
Division of Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan.
Tamura
M
M
Fukushima
S
S
Takeuchi
Y
Y
Sakuma
I
I
Kitabatake
A
A
D000818:Animals; D001785:Blood Gas Monitoring, Transcutaneous; D001802:Blood Substitutes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000592:standards; D019069:Cell Respiration; D003576:Electron Transport Complex IV / Q000378:metabolism; D004655:Emulsions; D005078:Exchange Transfusion, Whole Blood; D005466:Fluorocarbons / Q000737:chemistry / Q000493:pharmacokinetics* / Q000592:standards; D008928:Mitochondria / Q000201:enzymology; D023421:Models, Animal; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D051381:Rats
D001802:Blood Substitutes; D004655:Emulsions; D005466:Fluorocarbons; D003576:Electron Transport Complex IV; D010100:Oxygen