[The intracellular signaling pathway initiated by ginsenosides in hematopoietic cells].
OBJECTIVE
To investigate that ginsenosides (GS) promotes proliferation of hematopoietic cells by virtue of the intracellular signaling pathway.


METHODS
Three human megakaryocytic cell lines and Northern blot analysis, electrophoretic mobility shift assay (EMSA), antibody gel supershift assay and UV-radiation cross-linking were used.


RESULTS
After treatment with GS, GATA-2 mRNA levels in CHRH-288, Meg-01 and MO7e cells were increased by 1.84-, 2.43- and 1.52-fold, respectively, while GATA-1 mRNA levels remained as low as pretreatment. GATA binding activities were elevated in all the three GS treated cell lines. The GATA binding complex appeared as a band with an approximate molecular weight of 50,000 corresponding to the size of GATA-2 protein.


CONCLUSIONS
The intracellular signaling pathway initiated by GS was involved in transcription factor GATA-2. GATA-2 might play the key role in the GS induced upregulation of megakaryopoiesis.
Gao
R
R
Affiliated Hospital, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310006.
Wu
C
C
Chong
B H
BH
D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D050989:GATA2 Transcription Factor; D036145:Ginsenosides; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; C494711:GATA2 protein, human; D036145:Ginsenosides; D012333:RNA, Messenger; D012503:Saponins; D014157:Transcription Factors