title: In vivo acetylation of HMG1 protein enhances its binding affinity to distorted DNA structures.
abstract: The postsynthetic acetylation of HMG1 protein has been known for more than 20 years, but the effect of this modification on the properties of the protein has not been studied so far. Acetylated HMG1 was isolated from cells grown in the presence of sodium n-butyrate and identified as a monoacetylated protein, modified at lysine 2. Acetylated and parental forms of HMG1 were compared relative to their binding affinity to distorted DNA structures. By using mobility shift assay to determine the dissociation constants, we show that acetylation enhanced the ability of HMG1 to recognize UV light- or cisplatin-damaged DNA and four-way junctions. Since the modified lysine lies adjacent to the HMG1 DNA-binding domain, the results obtained were attributed to acetylation-induced conformational change in HMG1. The potential role of acetylation in modulating the interactions of HMG1 with both damaged DNA and other proteins is discussed.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria, and Institut de Biologie Structurale, Grenoble, France.
authors.forename: I
authors.initials: I
authors.lastname: Ugrinova
authors.forename: E A
authors.initials: EA
authors.lastname: Pasheva
authors.forename: J
authors.initials: J
authors.lastname: Armengaud
authors.forename: I G
authors.initials: IG
authors.lastname: Pashev
mesh_terms: D000107:Acetylation; D000818:Animals; D001202:Ascitic Fluid / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D017931:DNA Primers / Q000737:chemistry; D024243:HMGB1 Protein / Q000378:metabolism*; D013058:Mass Spectrometry; D009368:Neoplasm Transplantation; D009841:Oligonucleotides / Q000378:metabolism / Q000528:radiation effects; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D017931:DNA Primers; D024243:HMGB1 Protein; D009841:Oligonucleotides; D004247:DNA; D002945:Cisplatin