CheR- and CheB-dependent chemosensory adaptation system of Rhodobacter sphaeroides.
Rhodobacter sphaeroides has multiple homologues of most of the Escherichia coli chemotaxis genes, organized in three major operons and other, unlinked, loci. These include cheA(1) and cheR(1) (che Op(1)) and cheA(2), cheR(2), and cheB(1) (che Op(2)). In-frame deletions of these cheR and cheB homologues were constructed and the chemosensory behaviour of the resultant mutants examined on swarm plates and in tethered cell assays. Under the conditions tested, CheR(2) and CheB(1) were essential for normal chemotaxis, whereas CheR(1) was not. cheR(2) and cheB(1), but not cheR(1), were also able to complement the equivalent E. coli mutants. However, none of the proteins were required for the correct polar localization of the chemoreceptor McpG in R. sphaeroides. In E. coli, CheR binds to the NWETF motif on the high-abundance receptors, allowing methylation of both high- and low-abundance receptors. This motif is not contained on any R. sphaeroides chemoreceptors thus far identified, although 2 of the 13 putative chemoreceptors, McpA and TlpT, do have similar sequences. This suggests that CheR(2) either interacts with the NWETF motif of E. coli methyl-accepting chemotaxis proteins (MCPs), even though its native motif may be slightly different, or with another conserved region of the MCPs. Methanol release measurements show that R. sphaeroides has an adaptation system that is different from that of Bacillus subtilis and E. coli, with methanol release measurable on the addition of attractant but not on its removal. Intriguingly, CheA(2), but not CheA(1), is able to phosphorylate CheB(1), suggesting that signaling through CheA(1) cannot initiate feedback receptor adaptation via CheB(1)-P.
Martin
A C
AC
Microbiology Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom.
Wadhams
G H
GH
Shah
D S
DS
Porter
S L
SL
Mantotta
J C
JC
Craig
T J
TJ
Verdult
P H
PH
Jones
H
H
Armitage
J P
JP
D000220:Adaptation, Biological / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002451:Cell Compartmentation; D002630:Chemotactic Factors / Q000378:metabolism*; D002633:Chemotaxis / Q000502:physiology*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D000071677:Histidine Kinase; D008565:Membrane Proteins / Q000302:isolation & purification; D000432:Methanol / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D008780:Methyltransferases / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D012242:Rhodobacter sphaeroides / Q000502:physiology*; D015398:Signal Transduction
D001426:Bacterial Proteins; D002630:Chemotactic Factors; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C069656:CheB protein, Bacteria; D008780:Methyltransferases; C038706:chemotaxis methyltransferase; D000071677:Histidine Kinase; C000607608:cheA protein, E coli; D000432:Methanol