title: PrP genotype in Sarda breed sheep and its relevance to scrapie. Brief report.
abstract: Several PrP gene polymorphisms modulate sheep scrapie susceptibility. Recently, an increase of scrapie outbreaks has been reported in Italy. A vaccine containing sheep brain homogenate was used in most of the outbreaks. We investigated PrP gene polymorphisms in scrapie-affected and clinically healthy Sarda breed sheep from a flock exposed to the aforementioned vaccine, and in affected Sarda sheep from unexposed flocks. All affected animals were (Gln/Gln)171 homozygous. Moreover, we observed no variation for Ala136 and a new polymorphism (Lys to Asn) at codon 176. Our findings confirm the correlation between scrapie and (Gln/Gln)171 in breeds with no variation for Ala136.
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Vaccari
authors.forename: R
authors.initials: R
authors.lastname: Petraroli
authors.forename: U
authors.initials: U
authors.lastname: Agrimi
authors.forename: C
authors.initials: C
authors.lastname: Eleni
authors.forename: M G
authors.initials: MG
authors.lastname: Perfetti
authors.forename: M A
authors.initials: MA
authors.lastname: Di Bari
authors.forename: L
authors.initials: L
authors.lastname: Morelli
authors.forename: C
authors.initials: C
authors.lastname: Ligios
authors.forename: L
authors.initials: L
authors.lastname: Busani
authors.forename: R
authors.initials: R
authors.lastname: Nonno
authors.forename: G
authors.initials: G
authors.lastname: Di Guardo
mesh_terms: D000818:Animals; D001483:Base Sequence; D003062:Codon; D020022:Genetic Predisposition to Disease; D005838:Genotype; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D011817:Rabbits; D012608:Scrapie / Q000235:genetics*; D012756:Sheep
chemical_list: D003062:Codon; D011328:Prions