Automation of fluorescence-based PCR for confirmation of meningococcal disease.
A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.
Diggle
M A
MA
Scottish Meningococcus and Pneumococcus Reference Laboratory, Glasgow, United Kingdom.
Edwards
G F
GF
Clarke
S C
SC
D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins; D005453:Fluorescence; D006801:Humans; D008589:Meningococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012371:Robotics; D014157:Transcription Factors
D001426:Bacterial Proteins; C097706:CtrA protein, Caulobacter; D004268:DNA-Binding Proteins; D014157:Transcription Factors