title: Blocking effect of puerarin on calcium channel in isolated guinea pig ventricular myocytes.
abstract: OBJECTIVE
  To identify whether Puerarin (Puer) has blocking effects on L-type calcium channel.
  
  
  METHODS
  We used whole-cell recording of patch-clamp techniques to analyse calcium channel current in ventricular myocytes isolated from Langendorff guinea pig hearts by collagenase.
  
  
  RESULTS
  In control group, peak calcium channel current was decreased by 10.6% and 29.8% at 5, 10 min of recording time, respectively. For the same period, 0.1 mmol/L Puer reduced calcium current by 23.9% and 72.4%. 0.1 and 1 mmol/L Puer inhibited the amplitude of peak Calcium channel current by 40.6% and 63.2% after perfusing for 10 min. Compared with control group in which "rundown" phenomenon was observed, Puer also demonstrated its blocking effect on L-type channel. While washout of Puer with perfusing solution, calcium channel current went down further instead of recovery. Current-voltage (I-V) curves showed that the levels of calcium channel current were obviously decreased by Puer (both 0.1 mmo/L and 1 mmo/L) form -40 mV to +10 mV.
  
  
  CONCLUSIONS
  Puer has blocking effect on L-type calcium channel in a concentration dependent manner.
authors.affiliation: Cardiology Department, First People's Hospital, 85 Wujin Road, Shanghai 200080, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qian
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects*; D002469:Cell Separation; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology; D007529:Isoflavones / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007529:Isoflavones; C033607:puerarin