Carbenoxolone, a new inducer of heat shock protein 70.
Heat shock protein 70 (Hsp70) is capable of protecting cells, tissues, organs, and animals from a subsequent, normally lethal heating, as well as from numerous disease states. Therefore, it would be of great therapeutic benefit to discover compounds that are clinically safe yet able to induce Hsp70 in patients. Carbenoxolone, an antiulcer drug, protects gastric mucosal cells against irritants in vivo and in vitro. We assessed here whether carbenoxolone induces Hsp70 expression in human cell lines. We found that carbenoxolone increased the expression of Hsp70 protein and mRNA, and Hsp70 promoter activity.
Nagayama
S
S
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Jono
H
H
Suzaki
H
H
Sakai
K
K
Tsuruya
E
E
Yamatsu
I
I
Isohama
Y
Y
Miyata
T
T
Kai
H
H
D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002229:Carbenoxolone / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells; D000076249:Heat Shock Transcription Factors; D006801:Humans; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors
D000897:Anti-Ulcer Agents; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D002229:Carbenoxolone