title: High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical validation by UV, NMR and chemical ionisation-MS methods.
abstract: Methods are described for the optimised extraction, desulphation and HPLC separation of desulphoglucosinolates. These methods provide rapid separation, identification and quantitative measurements of glucosinolates extracted from Brassica napus L and related crops, of unusual glucosinolates found in crucifer weed species, and also of synthetic alkylglucosinolates. The desulphoglucosinolates used in these studies were either chemically synthesised (at least one example from each major structural class), or purified from various plant sources. Validation of the identities of the desulphoglucosinolates was by comparison of retention times with standards, and by UV, 1H- and 13C-NMR and chemical ionisation MS analysis. A list of useful species, and the specific tissues, from which high concentrations of standards can be extracted is included.
authors.affiliation: Crop Performance and Improvement Division, IACR-Rothamsted, Harpenden, Hertfordshire AL5 2JQ, UK. guy.kiddle@bbsrc.ac.uk
authors.forename: G
authors.initials: G
authors.lastname: Kiddle
authors.forename: R N
authors.initials: RN
authors.lastname: Bennett
authors.forename: N P
authors.initials: NP
authors.lastname: Botting
authors.forename: N E
authors.initials: NE
authors.lastname: Davidson
authors.forename: A A
authors.initials: AA
authors.lastname: Robertson
authors.forename: R M
authors.initials: RM
authors.lastname: Wallsgrove
mesh_terms: D029688:Brassica napus / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005961:Glucosinolates / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D005961:Glucosinolates