title: The effect of three different doses of sodium pentosan polysulphate on haematological and haemostatic variables in adult horses.
abstract: OBJECTIVE
  To evaluate the effects of three different doses of sodium pentosan polysulphate (PPS) on haematological and haemostatic variables in adult horses.
  
  
  METHODS
  Eight adult standardbred horses were used. All horses received a single injection of 0, 3, 6, and 10 mg/kg of PPS at the beginning of each treatment week for 4 weeks so that by the end of the study all horses had received all four doses of PPS. Blood samples were collected at 0, 1, 2, 3, 4, 6, 8, 12, 24, 48, and 168 h after each weekly injection of PPS. Variables measured were packed cell volume, haemoglobin, red blood cell count, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelet count, white cell count, neutrophil count, lymphocyte count, eosinophil count, monocyte count, serum protein, fibrinogen, prothrombin time, and activated partial thromboplastin time (PTT). Data were analysed using an ANOVA. Significance was set at P < 0.05.
  
  
  RESULTS
  There was a dose-dependent increase in PTT. A significant increase in PTT occurrred in all treatment groups when compared to horses receiving 0 mg/kg in which there was no change over time. The PTT values all returned to baseline by 48 h after treatment. The mean neutrophil count was higher 3 h after treatment when compared to time 0. Horses receiving 3 mg/kg of PPS had a higher lymphocyte count 4 h after injection, and those receiving 6 and 10 mg/kg had higher counts at 3,4,6 and 8 h after injection when compared to time 0. At 8 h after injection horses receiving 6 and 10 mg PPS had higher lymphocyte counts than horses not receiving PPS.
  
  
  CONCLUSIONS
  PPS causes a dose-dependent prolongation of PTT in horses. At the dose rates currently recommended for treatment of joint problems in horses this increase was small and remained elevated from baseline for up to 24 h. Based on these findings doses of PPS up to 3 mg/kg should not be administered to horses within 24 h of high stress activities or where physical injury may occur.
authors.affiliation: The University Veterinary Centre Camden, Department of Veterinary Clinical Sciences, University of Sydney, New SouthWales.
authors.forename: A
authors.initials: A
authors.lastname: Dart
authors.forename: N
authors.initials: N
authors.lastname: Perkins
authors.lastname: Dowling
authors.forename: T
authors.initials: T
authors.lastname: Batterham
authors.forename: C
authors.initials: C
authors.lastname: Livingston
authors.forename: D
authors.initials: D
authors.lastname: Hodgson
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count / Q000662:veterinary; D004909:Erythrocyte Indices / Q000662:veterinary; D005260:Female; D006403:Hematologic Tests / Q000662:veterinary*; D006454:Hemoglobins / Q000032:analysis; D006736:Horses / Q000097:blood*; D007958:Leukocyte Count / Q000662:veterinary*; D008297:Male; D010426:Pentosan Sulfuric Polyester / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D006454:Hemoglobins; D010426:Pentosan Sulfuric Polyester