title: Tissue-engineered bone repair of sheep cranial defects with autologous bone marrow stromal cells.
abstract: Cranial bone defect remains a major challenge to craniofacial surgeons because of limited availability of autologous bone graft to repair the defects and the donor site defects secondary to tissue harvesting. In contrast, tissue-engineering technique can generate a large bone tissue using small amount of autologous cells and therefore avoid these problems. Bone Marrow Stromal Cells (MSCs) have the potential of multi-lineage (including osteogenic) differentiation. The objective of this study was to investigate the potential of using autologous MSCs to repair cranial bone defects by a tissue-engineering approach. Autologous MSCs were isolated from eight adult sheep respectively and were in vitro expanded and induced to become osteogenic cells. Bilateral full-thickness defects (20 mm in diameter) of parietal bones were created in animals and the bone defects were either repaired with the bone implants constituted with MSCs and calcium alginate at the experimental side (n = 8) or treated with calcium alginate only without MSCs (n = 4) or left unrepaired (n = 4) at the control side. New bone tissues were observed either grossly or histologically at the defects of experimental group as early as 6 weeks post-repairing, but not in control groups. The engineered bone tissue became more mature at 18 weeks post-repairing. Three-dimensional computerized tomography (CT) scan revealed an almost complete repair of the defect of experimental group at 18 weeks. This study may provide insight for future clinical repair of cranial defect.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Tissue Engineering Center, Shanghai Second Medical University, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Shang
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: L
authors.initials: L
authors.lastname: Cui
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000464:Alginates; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001847:Bone Diseases / Q000601:surgery*; D016026:Bone Marrow Transplantation; D002118:Calcium / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D024042:Collagen Type I / Q000032:analysis; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D021621:Imaging, Three-Dimensional; D010006:Osteoblasts / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000502:physiology; D010294:Parietal Bone / Q000601:surgery*; D014677:Pharmaceutical Vehicles; D012756:Sheep; D016622:Silver Staining; D013223:Statistics as Topic; D017154:Stromal Cells / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods*; D014057:Tomography, X-Ray Computed; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: D000464:Alginates; D024042:Collagen Type I; D006603:Hexuronic Acids; D014677:Pharmaceutical Vehicles; D015675:Osteocalcin; D020723:Glucuronic Acid; D000469:Alkaline Phosphatase; D002118:Calcium