Quantification of TECTA and DFNA5 expression in the developing mouse cochlea.
TECTA and DFNA5 are the mouse orthologues of the human deafness-associated genes TECTA and DFNA5. To determine how expression of these genes is regulated during development, relative mRNA abundance was examined in mice by non-radioactive RT-PCR. TECTA mRNA was detected on embryonic day 15 (E15), increased to its highest level on postnatal day 3 (P3) and then dramatically decreased by P15. Low levels persisted (adulthood, P45 to 67) with mean mRNA abundance after P15 less than 25% of P3 levels. DFNA5 mRNA expression was constant throughout these time points. These results imply that TECTA is transcribed at a particularly high level during tectorial membrane morphogenesis. In contrast, DFNA5 is present in both the developing and mature cochlea.
Maeda
Y
Y
Department of Otolaryngology- Head and Neck Surgery, Okayama University Medical School, 2-5-1 Shikata-Cho, Okayama, 700-8558 Japan.
Fukushima
K
K
Kasai
N
N
Maeta
M
M
Nishizaki
K
K
D000375:Aging / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D002352:Carrier Proteins / Q000235:genetics*; D003051:Cochlea / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D003638:Deafness / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005260:Female; D005333:Fetus; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism*; D011960:Receptors, Estrogen; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000094524:Gasdermins
D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D058851:GPI-Linked Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C491747:Gsdme protein, mouse; D000094524:Gasdermins; C491350:TECTA protein, human; C105350:Tecta protein, mouse