title: Characterization of recombinant Desulfovibrio gigas ferredoxin.
abstract: Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared.
authors.affiliation: Departamento de Química, Centro Quìmica Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825 Monte de Caparica, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Rodrigues
authors.forename: F
authors.initials: F
authors.lastname: Graça
authors.forename: A L
authors.initials: AL
authors.lastname: Macedo
authors.forename: I
authors.initials: I
authors.lastname: Moura
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
mesh_terms: D003001:Cloning, Molecular; D003901:Desulfovibrio / Q000737:chemistry*; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D005288:Ferredoxins; D011994:Recombinant Proteins; D013455:Sulfur; D007501:Iron; D010100:Oxygen