[Rapid immunohistochemistry applied to intraoperative diagnosis: a critical analysis].
A series of 1378 intra-operative frozen diagnoses performed in our department over two years (1998-1999) was studied in order to assess the agreement with the diagnosis on formalin-fixed, paraffin-embedded sections. In selected cases, a rapid immunostaining (IS) method was applied on frozen sections to solve diagnostic problems. Rapid IS allowed us to determine the histotype of poorly differentiated neoplasms, the origin of lung nodules and the presence of nests of neoplastic cells on resection margins. The method delayed the answer by about 20 minutes, and was always previously arranged with the surgeon. Rapid IS method was performed using routine reagents and was not repeated on formalin-fixed, paraffin-embedded sections of the same cases, thus avoiding additional costs.
Viberti
L
L
Sezione di Anatomia Patologica, Dipartimento di Scienze Biomediche e Oncologia Umana, Università degli Studi di Torino, Via Santena 7, I-10126 Torino, Italia.
Croce
S
S
Pecchioni
C
C
Bongiovanni
M
M
Sapino
A
A
D014408:Biomarkers, Tumor / Q000032:analysis*; D019921:Biotinylation; D018288:Carcinoma, Small Cell / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology; D002454:Cell Differentiation; D003937:Diagnosis, Differential; D005260:Female; D005629:Frozen Sections; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D007432:Intraoperative Period; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D016612:Paraffin Embedding; D012189:Retrospective Studies; D021701:Sentinel Lymph Node Biopsy; D013997:Time Factors
D014408:Biomarkers, Tumor