title: Analysis of the rpoN locus in the plant pathogenic bacterium, Pseudomonas syringae pv. glycinea.
abstract: sigma 54, which is encoded by rpoN, is required for a variety of metabolic functions in bacteria including the utilization of alternative carbon and nitrogen sources, nitrogen fixation, and the expression of virulence determinants. Sequence analysis of a 3,020-bp DNA fragment from the plant pathogen Pseudomonas syringae pv. glycinea PG4180 revealed four ORFs designated rpoN, orfA, orfB, and orfC delta, which were related to rpoN and rpoN-associated genes from other microorganisms. The rpoN upstream region in P. syringae contained two overlapping promoters, which may suggest a complex regulatory pattern. This is the first study describing the organization of the rpoN locus in P. syringae.
authors.affiliation: 127 Noble Research Center, Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Alarcón-Chaidez
authors.forename: C L
authors.initials: CL
authors.lastname: Bender
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010944:Plants / Q000382:microbiology; D011549:Pseudomonas / Q000235:genetics* / Q000472:pathogenicity; D051877:RNA Polymerase Sigma 54; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54