title: Evaluation of ColorPAC Giardia/Cryptosporidium rapid assay and ProSpecT Giardia/Cryptosporidium microplate assay for detection of Giardia and Cryptosporidium in fecal specimens.
abstract: Detection of Giardia and Cryptosporidium in clinical stool specimens using the ColorPAC and ProSpecT enzyme immunoassays revealed 98.7% agreement for Giardia detection and 98.1% agreement for Cryptosporidium detection. Sensitivities were uniformly 100%. The specificities of the ColorPAC immunoassay for Giardia and Cryptosporidium detection were 100 and 99.5%, respectively, and those for the ProSpecT assay were 98.4 and 98.6%, respectively. The false-positive reactions with the ProSpecT assay occurred with specimens that were grossly bloody.
authors.affiliation: Section of Clinical Microbiology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Katanik
authors.forename: S K
authors.initials: SK
authors.lastname: Schneider
authors.forename: J E
authors.initials: JE
authors.lastname: Rosenblatt
authors.forename: G S
authors.initials: GS
authors.lastname: Hall
authors.forename: G W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis* / Q000469:parasitology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic