title: Flow cytometry of Escherichia coli on microfluidic devices.
abstract: Flow cytometry of the bacterium Escherichia coli was demonstrated on a microfabricated fluidic device (microchip). The channels were coated with poly(dimethylacrylamide) to prevent cell adhesion, and the cells were transported electrophoretically by applying potentials to the fluid reservoirs. The cells were electrophoretically focused at the channel cross and detected by coincident light scattering and fluorescence. The E. coli were labeled with a membrane-permeable nucleic acid stain (Syto15), a membrane-impermeable nucleic acid stain (propidium iodide), or a fluorescein-labeled antibody and counted at rates from 30 to 85 Hz. The observed labeling efficiencies for the dyes and antibody were greater than 94%.
authors.affiliation: Oak Ridge National Laboratory, Tennessee 37831-6142, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: McClain
authors.forename: C T
authors.initials: CT
authors.lastname: Culbertson
authors.forename: S C
authors.initials: SC
authors.lastname: Jacobson
authors.forename: J M
authors.initials: JM
authors.lastname: Ramsey
mesh_terms: D002463:Cell Membrane Permeability; D004586:Electrophoresis / Q000379:methods; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D009995:Osmosis
chemical_list: D005456:Fluorescent Dyes