An mRNA degrading complex in Rhodobacter capsulatus.
An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
Jäger
S
S
Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Fuhrmann
O
O
Heck
C
C
Hebermehl
M
M
Schiltz
E
E
Rauhut
R
R
Klug
G
G
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D002458:Cell Fractionation; D002498:Centrifugation / Q000379:methods; D004722:Endoribonucleases / Q000276:immunology / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D020365:RNA Helicases / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020539:Sequence Analysis, Protein
D000906:Antibodies; D046911:Macromolecular Substances; D012333:RNA, Messenger; D004722:Endoribonucleases; C022537:ribonuclease E; D020365:RNA Helicases