Genetic and cytological characterization of a developmental mutant of Aspergillus nidulans induced by 5-azacytidine.
An analysis of a new medusa mutant of Aspergillus nidulans obtained by 5-azacytidine-treatment and named B116 is provided. The B116 mutant was phenotypically characterized by the production of conidiophores with reduced pigmentation and vesicles bearing multiple tiers of sterigmata. A single nuclear gene located on chromosome I is responsible for phenotypical changes in the mutant. The 5-azacytidine-altered locus, designated medA102, is recessive in heterozygous diploid and the medusa mutant is a Dp(II,I) duplication bearer that renders the strain mitotically unstable.
Costa
M A
MA
Universidade Paranaense, Instituto de Ciências Farmacêuticas e Bioquímica, Praça Mascarenhas de Morais S/N, Umuarama, Paraná, Brasil, 87500-000.
Silva
N C
NC
Castro-Prado
M A
MA
D000483:Alleles; D000818:Animals; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D001374:Azacitidine / Q000494:pharmacology; D020090:Chromosome Segregation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005800:Genes, Fungal / Q000187:drug effects*; D008540:Meiosis; D008938:Mitosis; D009154:Mutation; D010641:Phenotype; D037401:Scyphozoa
D004791:Enzyme Inhibitors; D001374:Azacitidine