In silico screening for tumour-specific expressed sequences in human genome.
A computer-based differential display tool named HsAnalyst has been developed and successfully used for the comparison of expression patterns in a set of tumours versus a set of normal tissues. A list of EST clusters highly represented in tumours and rarely observed in normal tissues has been developed as a resulting output file of the program. These differentially expressed EST clusters (genes) can be useful for developing new tumour markers and prognostic indicators for a wide set of human malignancies. Tumour-specific protein-coding genes may be considered a manifestation of tumour-specific gene expression.
Baranova
A V
AV
Vavilov Institute of General Genetics, 3 Gubkina Str., Moscow 119991, Russia. baranova@vigg.ru
Lobashev
A V
AV
Ivanov
D V
DV
Krukovskaya
L L
LL
Yankovsky
N K
NK
Kozlov
A P
AP
D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D052138:Genes, Neoplasm; D015894:Genome, Human; D006801:Humans; D009369:Neoplasms / Q000235:genetics*