[Polymorphisms of apoB gene 3' VNTR alleles and analysis of lipid level in Tibetan population].
OBJECTIVE
To study the distribution and frequency of apoB gene 3' end alleles and try to find if there is any association with the level of plasma lipids.


METHODS
PCR and gradient polyacrylamide gel electrophoresis were used to analysize the chromsome DNA and PCR products.


RESULTS
Totally 20 alleles were found in the Tibetan group and the distribution of alleles was bimode. The HVE32 and HVE34 were most common, and the frequencies of HVE32 and HVE34 were 0.27 and 0.21 respectively.


CONCLUSIONS
Polymorphism apoB gene resides not only in the variable numbers of the repeat unit, but also in the variation in the oligonucleotide sequence within the repeat units as well as the variation in the arrangement of the repeat units.
Chen
B
B
Laboratory of National Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005.
Zhao
Y
Y
Cheng
Y
Y
D000293:Adolescent; D000328:Adult; D000483:Alleles; D001055:Apolipoproteins B / Q000235:genetics*; D002681:China; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic
D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D002784:Cholesterol