Neural circuitry of the kidney: NO-containing neurons.
The neurons synthesizing nitric oxide (NO) that are part of the renal sympathetic pathways were located by double-staining for the neuronal isoform of nitric oxide synthase (nNOS) using immunocytochemistry to identify NO-synthesizing neurons and transneuronal tracing following infection of the left kidney with pseudorabies virus (PRV). Following kidney injection with PRV, the animals survived 4-day post-inoculation prior to sacrifice and tissue processing. PRV-infected neurons that double-stained for nNOS were found in the paraventricular hypothalamic nucleus (PVN), the raphe obscurus nucleus (ROb), the ventromedial medulla (VMM), the rostral ventrolateral medulla (rVLM) and the A5 cell group. In the thoracolumbar spinal cord, nNOS neurons co-localized with PRV-infected cells in the dorsal horn in laminae I, III-V ipsilateral to the injected kidney and in lamina X, the intermediolateral cell column, the lateral funiculus, the intercalated nucleus and the central autonomic area. We conclude that NO synthesizing cells may significantly affect renal autonomic pathways in the rat by interacting with the renal sensory and sympathomotor circuitry at multiple sites.
Weiss
M L
ML
Departments of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5602, USA. weiss@vet.ksu.edu
Chowdhury
S I
SI
Patel
K P
KP
Kenney
M J
MJ
Huang
J
J
D000344:Afferent Pathways / Q000166:cytology / Q000378:metabolism / Q000821:virology; D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D004525:Efferent Pathways / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D011558:Herpesvirus 1, Suid / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000294:innervation* / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism / Q000821:virology; D026602:Nitrergic Neurons / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D009569:Nitric Oxide / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism / Q000821:virology; D011149:Pons / Q000166:cytology / Q000378:metabolism / Q000821:virology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D013564:Sympathetic Nervous System / Q000166:cytology / Q000378:metabolism* / Q000821:virology
D009569:Nitric Oxide