Retina-specific cis-elements and binding nuclear proteins of carp rhodopsin gene.
The upstream cis-elements controlling the retina-specific expression of carp rhodopsin gene were fully characterized in vivo. Transgenic studies demonstrated that both carp neural retina leucine zipper response-like element (cNRE, within nucleotides (nt) -63 to -75) and carp-specific element (CSE, nt -46 to -52) were crucial to reporter gene expression in medaka retinae. The retina-specific expression rates of embryos injected with nt -1 to -641 and longer fragments were much higher than those of embryos injected with nt -1 to -138 and shorter fragments, indicating that an enhancer is located in the nt -138 to -641 region. Retinal extracts and the probe BAT-1 (nt -90 to -120) formed two DNA-protein complexes, B1 and B2. Retinal extracts and the probes cNRE and CSE formed the complexes N1 and C1, respectively. The protein factors in B1 and C1 were mammal-like cone-rod homeobox proteins.
Ma
G C
GC
Institute of Fisheries Science, National Taiwan University, Taipei.
Wang
T M
TM
Su
C Y
CY
Wang
Y L
YL
Chen
S
S
Tsai
H J
HJ
D000818:Animals; D030801:Animals, Genetically Modified; D002347:Carps / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005136:Eye Proteins / Q000032:analysis; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000032:analysis; D007150:Immunohistochemistry; D008164:Luminescent Proteins / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D020218:Response Elements / Q000235:genetics*; D012160:Retina / Q000378:metabolism*; D012243:Rhodopsin / Q000235:genetics*; D015534:Trans-Activators / Q000032:analysis; D019076:Transgenes / Q000235:genetics
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; C109288:cone rod homeobox protein; D049452:Green Fluorescent Proteins; D004247:DNA; D012243:Rhodopsin