Controlled expression in Klebsiella pneumoniae and Shigella flexneri using a bacteriophage P1-derived C1-regulated promoter system.
The utility of promoters regulated by the bacteriophage P1 temperature-sensitive C1 repressor was examined in Shigella flexneri and Klebsiella pneumoniae. Promoters carrying C1 operator sites driving LacZ expression had induction/repression ratios of up to 240-fold in S. flexneri and up to 50-fold in K. pneumoniae. The promoters exhibited remarkably low basal expression, demonstrated modulation by temperature, and showed rapid induction. This system will provide a new opportunity for controlled gene expression in enteric gram-negative bacteria.
Schofield
D A
DA
Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Ave., Charleston, SC 29403, USA. schofida@musc.edu
Westwater
C
C
Dolan
J W
JW
Schmidt
M G
MG
Norris
J S
JS
D001483:Base Sequence; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000502:physiology*; D012763:Shigella flexneri / Q000235:genetics*; D013696:Temperature; D014764:Viral Proteins / Q000502:physiology*; D001616:beta-Galactosidase / Q000378:metabolism
D012097:Repressor Proteins; D014764:Viral Proteins; C076896:repressor protein C1, bacteriophage; D001616:beta-Galactosidase