Cold stress induced high molecular weight membrane polypeptides are responsible for cold tolerance in Rhizobium DDSS69.
Cold stress induces a lag phase in the growth cycle of Rhizobium DDSS69. Two cold sensitive mutants of DDSS69 were generated through Tn5 tagged mutagenesis. These mutants do not grow below 15 degrees C but show a growth curve comparable with the wild type grown at 5 degrees C. There is a rapid induction of two high molecular weight membrane polypeptides of 135 and 119 kDa within 15 min of exposure to 5 degrees C in DDSS69. PAGE membrane protein profiles of stressed and non-stressed cells reveal differential regulation of genes. At 15 degrees C both mutants lack the high molecular weight polypeptides, suggesting a role in alleviation of cold stress.
Sardesai
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N
Department of Botany, University of Delhi, India. nageshsardesai@hotmail.com
Babu
C R
CR
D000064:Acclimatization; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D008970:Molecular Weight; D016296:Mutagenesis; D009154:Mutation; D010452:Peptide Biosynthesis / Q000235:genetics / Q000502:physiology*; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
D001426:Bacterial Proteins