title: Restricted expression of LUZP in neural lineage cells: a study in embryonic stem cells.
abstract: A novel protein LUZP with 3 leucine zipper motifs at its amino terminus is predominantly expressed in the adult brain. A modified gene targeting approach was employed in an attempt to establish in vitro and in vivo models in which Luzp is knock-out (KO) for phenotype assessment and a reporter gene lacZ is knock-in (KI) for tracing its expression. We report in this study the molecular cloning of the Luzp gene, its targeting vector construction and Luzp-KO/lacZ-KI embryonic stem (ES) clone selection. Since LUZP is also expressed in ES cells, the possibility of embryonic lethality cannot be excluded when attempting to establish Luzp-null mutant mice. We have therefore examined the development of homozygous Luzp-KO/lacZ-KI clones in nude mice. Tissue types derived from all three embryonic germ layers were observed in teratomas developed in nude mice. In situ X-gal staining further revealed restricted expression of LUZP in neural lineage cells.
authors.affiliation: Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: M W
authors.initials: MW
authors.lastname: Lee
authors.forename: A C
authors.initials: AC
authors.lastname: Chang
authors.forename: D S
authors.initials: DS
authors.lastname: Sun
authors.forename: C Y
authors.initials: CY
authors.lastname: Hsu
authors.forename: N C
authors.initials: NC
authors.lastname: Chang
mesh_terms: D000818:Animals; D019070:Cell Lineage / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D005697:Galactosides; D018390:Gene Targeting; D005822:Genetic Vectors; D018380:Hematopoietic Stem Cell Transplantation; D007150:Immunohistochemistry; D007211:Indoles; D007763:Lac Operon / Q000235:genetics; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D011506:Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013234:Stem Cells / Q000378:metabolism; D013724:Teratoma / Q000737:chemistry / Q000473:pathology; D014018:Tissue Distribution
chemical_list: D004268:DNA-Binding Proteins; D005697:Galactosides; D007211:Indoles; C103593:Luzp1 protein, mouse; D011506:Proteins; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside