title: Use of magnetic beads for Gram staining of bacteria in aqueous suspension.
abstract: A Gram staining technique was developed using monodisperse magnetic beads in concentrating bacteria in suspension for downstream application. The technique does not require heat fixation of organisms, electrical power, or a microscope. Gram-negative and Gram-positive bacteria were identified macroscopically based on the colour of the suspension. The bacteria concentrated on magnetic beads may also be identified microscopically.
authors.affiliation: Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, P.O. Box 8146 Dep, N-0033, Oslo, Norway. siamak.p.yazdankhah@veths.no
authors.forename: S P
authors.initials: SP
authors.lastname: Yazdankhah
authors.forename: H
authors.initials: H
authors.lastname: Sørum
authors.forename: H J
authors.initials: HJ
authors.lastname: Larsen
authors.forename: G
authors.initials: G
authors.lastname: Gogstad
mesh_terms: D001431:Bacteriological Techniques; D003116:Color; D004396:Coloring Agents; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D008280:Magnetics; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents