Competition between citrate and heptapeptide DDSDEEN binding to DNA in presence of divalent cations.
The binding of citrate and acidic peptide DDSDEEN with DNA in the presence of divalent cations is compared. Citric acid shows a higher number of binding sites on the DNA compared to the peptide; this is probably due to the bigger sitric hindrance of the peptide compared to the citric acid for the binding in the DNA grooves. Moreover. DNA preincubated with saturating amounts of citric acid is not available for the binding with successively added peptide. Therefore the peptide and citrate binding sites to some extent overlap.
Calzuola
I
I
Dipartimento di Biologia Cellulare e Molecolare, Università, di Perugia, Italy.
Castigli
E
E
Gianfranceschi
G L
GL
Marsili
V
V
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002412:Cations; D019343:Citric Acid / Q000737:chemistry / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D008297:Male; D018716:Molecular Mimicry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D015032:Zinc / Q000378:metabolism
D002412:Cations; D010455:Peptides; D019343:Citric Acid; D003300:Copper; D004247:DNA; D007501:Iron; D015032:Zinc