title: In vivo visualization of the cochlear nerve and nuclei with fluorescent axonal tracers.
abstract: In recent years multichannel neuroprostheses have been developed which directly stimulate the central auditory pathway. Substantially these have been used in cases of total hearing loss caused by neurofibromatosis type 2 where bilateral damage to the auditory nerve prevents more peripheral stimulation. The electrode carrier of the auditory brainstem implant (ABI) is designed to be placed on the cochlear nucleus complex residing at the lateral brainstem surface. Despite altered anatomy due to tumor growth or preceding surgery, correct electrode placement is essential to maximize the variety of pitch percept elicited during electrical stimulation with the ABI without producing side-effects. In order to assist intraoperative identification of the proximal auditory nerve and cochlear nuclei, the non-toxic fluorescent axonal tracers Fast Blue or Fluorogold were injected into the cochlea of rats and Java monkeys. Four to seven days after tracer application, labeling of the eighth cranial nerve, its entrance into the brainstem and the primary radiation of auditory fibers into the cochlear nucleus could be demonstrated as colored fluorescence on the living brain under appropriate ultraviolet illumination. Additional histological processing revealed groups of retrogradely labeled neuronal cell bodies in both species. Our results suggest that this method could also be used in humans in order to aid surgeons with the proper positioning of the electrode array.
authors.affiliation: Neurobiological Research Laboratory, Department of Otorhinolaryngology, Albert-Ludwigs-University Freiburg, Germany. marangos@hno.klinik.uni-mainz.de
authors.forename: N
authors.initials: N
authors.lastname: Marangos
authors.forename: R B
authors.initials: RB
authors.lastname: Illing
authors.forename: J
authors.initials: J
authors.lastname: Krüger
authors.forename: R
authors.initials: R
authors.lastname: Laszig
mesh_terms: D000578:Amidines / Q000493:pharmacokinetics; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D001933:Brain Stem / Q000601:surgery; D003051:Cochlea / Q000601:surgery; D003056:Cochlear Nerve / Q000033:anatomy & histology* / Q000378:metabolism; D017626:Cochlear Nucleus / Q000033:anatomy & histology* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D000882:Haplorhini; D051381:Rats; D017208:Rats, Wistar; D013266:Stilbamidines; D013997:Time Factors
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000578:Amidines; D005456:Fluorescent Dyes; D013266:Stilbamidines; C031455:diamidino compound 253-50