Content of apoptotic enzyme caspase-3 mRNA in brain stem and cortex in rats during postnatal ontogeny.
The content of caspase-3 mRNA in rat brain stem decreased from birth to postnatal week 3 and dropped below reverse transcription-PCR sensitivity limit in 1.5-month-old animals. The number of brain stem cells in 2-40-day-old rats was constant. The content of caspase-3 mRNA in the cortex was higher than in the brain stem and decreased by one-third by postnatal day 40. The number of cells in the cortex decreased 2-fold during postnatal week 1 and then remained unchanged. Changes in the content of caspase-3 mRNA did not correlate directly with variations in the number of brain cells during postnatal ontogeny.
Kalinina
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TS
Laboratory of genetic basis of neuroendocrine regulate, Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Medical Science, Novosibirsk. kalin@ bionet.nsc.ru
Bannova
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AV
Dygalo
N N
NN
D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000254:growth & development*; D001933:Brain Stem / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D053148:Caspase 3; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D002540:Cerebral Cortex / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases