In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome.
OBJECTIVE
To investigate differences in immature oocyte maturation, fertilization, and pregnancy rates among women with unstimulated normal ovaries, polycystic ovaries (PCOs), or PCOS.


METHODS
Prospective observational study.


METHODS
University fertility clinic.


METHODS
One hundred forty-four women undergoing 180 in vitro oocyte maturation treatment cycles.


METHODS
Transvaginal immature oocyte recovery from unstimulated ovaries 36 hours after hCG priming. In vitro oocyte maturation and fertilization. Fresh embryo transfer.


METHODS
Immature oocytes collected, metaphase II oocytes, and embryos produced. Implantation and pregnancy rates.


RESULTS
The overall oocyte maturation and fertilization rates attained were 80.3% (1,222 of 1,522) and 76.5% (935 of 1,222), respectively. Significantly fewer immature oocytes were retrieved from normal ovaries (5.1 +/- 3.7) compared with the PCO (10.0 +/- 5.1) or PCOS (11.3 +/- 9.0) groups. Fertilization and cleavage rates were comparable among the three groups. The implantation, pregnancy, and live birth rates per transfer for normal ovaries were 1.5%, 4.0%, and 2.0%, respectively; for PCOs 8.9%, 23.1%, 17.3%, respectively; and for women with PCOS 9.6%, 29.9%, and 14.9%, respectively.


CONCLUSIONS
Immature oocytes retrieved from normal ovaries, PCOs, or women with PCOS, when using hCG priming before oocyte retrieval, have a similarly high maturation, fertilization, and cleavage potential. In vitro maturation is a useful treatment option, particularly for women with PCOs.
Child
T J
TJ
McGill Reproductive Center, Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada. timothychild@yahoo.com
Abdul-Jalil
A K
AK
Gulekli
B
B
Tan
S L
SL
D002478:Cells, Cultured; D016922:Cellular Senescence; D002970:Cleavage Stage, Ovum; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000502:physiology*; D010048:Ovarian Cysts / Q000503:physiopathology*; D010053:Ovary / Q000503:physiopathology*; D011085:Polycystic Ovary Syndrome / Q000503:physiopathology*; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D012016:Reference Values