Use of magnetic beads for Gram staining of bacteria in aqueous suspension.
A Gram staining technique was developed using monodisperse magnetic beads in concentrating bacteria in suspension for downstream application. The technique does not require heat fixation of organisms, electrical power, or a microscope. Gram-negative and Gram-positive bacteria were identified macroscopically based on the colour of the suspension. The bacteria concentrated on magnetic beads may also be identified microscopically.
Yazdankhah
S P
SP
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, P.O. Box 8146 Dep, N-0033, Oslo, Norway. siamak.p.yazdankhah@veths.no
Sørum
H
H
Larsen
H J
HJ
Gogstad
G
G
D001431:Bacteriological Techniques; D003116:Color; D004396:Coloring Agents; D006090:Gram-Negative Bacteria; D006094:Gram-Positive Bacteria; D008280:Magnetics; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents