Degradation of cellulose by nitrogen-fixing strain of Bacillus polymyxa.
During an intensive screening programme, several strains of cellulolytic bacteria were isolated. One nitrogenase-positive strain able to degrade filter paper, Avicel cellulose, carboxymethyl cellulose and cellobiose was selected for further study. On the basis of biochemical characteristics and Mol % of G+C content, the selected strain was identified as Bacillus polymyxa. The highest production of the enzymes degrading filter paper (FP-ase) and carboxymethyl cellulose (CMCase) by B. polymyxa was observed in Park's medium suplemented with Avicel cellulose. The investigated strain of bacteria produced cellulosome-like structures as was shown by transmission electron microscopy.
Górska
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E
Department of Agricultural Microbiology, Warsaw Agricultural University, 02-528 Warsaw, 26/30 Rakowiecka.
Tudek
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B
Russel
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S
D001407:Bacillus / Q000201:enzymology* / Q000648:ultrastructure; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism*; D008854:Microscopy, Electron; D009591:Nitrogenase / Q000378:metabolism; D010209:Paper
D009591:Nitrogenase; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium