title: Characterisation of Eubacterium-like strains isolated from oral infections.
abstract: The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. In this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the genus Eubacterium were subjected to phenotypic and genotypic analysis. Ninety-one of the isolates were identified as belonging to one of 14 previously described species: Atopobium parvulum (5 isolates), A. rimae (29), Bulleidia extructa (2), Cryptobacterium curtum (1), Dialister pneumosintes (1), Eubacterium saburreum (2), E. sulci (8), E. yurii subsp. yurii (1), Filifactor alocis (3), Lactobacillus uli (1), Mogibacterium timidum (13), M. vescum (6), Pseudoramibacter alactolyticus (6) and Slackia exigua (13). The remaining 14 isolates did not correspond to existing species. This study confirms the diversity of organisms provisionally assigned to the genus Eubacterium by conventional identification methods. This group of organisms is frequently isolated from oral infections but their role in the aetiology of these conditions has yet to be determined.
authors.forename: Julia
authors.initials: J
authors.lastname: Downes
authors.forename: Mark A
authors.initials: MA
authors.lastname: Munson
authors.forename: David A
authors.initials: DA
authors.lastname: Spratt
authors.affiliation: Molecular Microbial Ecology Programme, Guy's, King's and St Thomas' Dental Institute, King's College London, London SE1 9RT, UK and *Anaerobe Reference Unit, National Public Health Institute, 166 Manneheiminte, Helsinki, Finland.
authors.forename: Eija
authors.initials: E
authors.lastname: Kononen
authors.affiliation: Molecular Microbial Ecology Programme, Guy's, King's and St Thomas' Dental Institute, King's College London, London SE1 9RT, UK and *Anaerobe Reference Unit, National Public Health Institute, 166 Manneheiminte, Helsinki, Finland.
authors.forename: Eveliina
authors.initials: E
authors.lastname: Tarkka
authors.affiliation: Molecular Microbial Ecology Programme, Guy's, King's and St Thomas' Dental Institute, King's College London, London SE1 9RT, UK and *Anaerobe Reference Unit, National Public Health Institute, 166 Manneheiminte, Helsinki, Finland.
authors.forename: Hannele
authors.initials: H
authors.lastname: Jousimies-Somer
authors.forename: William G
authors.initials: WG
authors.lastname: Wade
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D015921:Dental Implants / Q000382:microbiology; D005051:Eubacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020459:Genes, rRNA; D005838:Genotype; D006801:Humans; D009059:Mouth Diseases / Q000382:microbiology*; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012463:Saliva / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D015921:Dental Implants; D012336:RNA, Ribosomal, 16S