title: Lipoprotein (a) binds and inactivates tissue factor pathway inhibitor: a novel link between lipoproteins and thrombosis.
abstract: Lipoprotein (a) [Lp(a)] has been associated with both anti-fibrinolytic and atherogenic effects. However, no direct link currently exists between this atherogenic lipoprotein and intravascular coagulation. The current study examined the binding and functional effects of Lp(a), its lipoprotein constituents, apoliprotein (a) [apo(a)] and low-density lipoprotein (LDL), and lysine-plasminogen (L-PLG), which shares significant homology with apo(a), on tissue factor pathway inhibitor (TFPI), a major regulator of tissue factor-mediated coagulation. Results indicate that Lp(a), apo(a), and PLG but not LDL bound recombinant TFPI (rTFPI) in vitro and that apo(a) bound to a region spanning the last 37 amino acid residues of the c-terminus of TFPI. The apparent binding affinity for TFPI was much higher for Lp(a) (KD approximately 150 nM) compared to PLG (KD approximately 800 nM) and nanomolar concentrations of apo(a) (500 nM) inhibited PLG binding to TFPI. Lp(a) also inhibited in a concentration-dependent manner rTFPI activity and endothelial cell surface TFPI activity in vitro, whereas PLG had no such effect. Moreover physiologic concentrations of PLG (2 microM) had no effect on the concentration-dependent inhibition of TFPI activity induced by Lp(a). In human atherosclerotic plaque, apo(a) and TFPI immunostaining were shown to coexist in smooth muscle cell-rich areas of the intima. These data suggest a novel mechanism whereby Lp(a) through its apo(a) moiety may promote thrombosis by binding and inactivating TFPI.
authors.affiliation: Department of Internal Medicine and Cardiovascular Diseases, Mayo Clinic and Foundation, Rochester, MN 55905, USA. caplice.noel@mayo.edu
authors.forename: N M
authors.initials: NM
authors.lastname: Caplice
authors.forename: C
authors.initials: C
authors.lastname: Panetta
authors.forename: T E
authors.initials: TE
authors.lastname: Peterson
authors.forename: L S
authors.initials: LS
authors.lastname: Kleppe
authors.forename: C S
authors.initials: CS
authors.lastname: Mueske
authors.forename: G M
authors.initials: GM
authors.lastname: Kostner
authors.forename: G J
authors.initials: GJ
authors.lastname: Broze
authors.forename: R D
authors.initials: RD
authors.lastname: Simari
mesh_terms: D000818:Animals; D001054:Apolipoproteins A / Q000378:metabolism; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology; D001665:Binding Sites; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004730:Endothelium, Vascular / Q000166:cytology; D005342:Fibrinolysis; D006801:Humans; D017270:Lipoprotein(a) / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008074:Lipoproteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000648:ultrastructure; D010446:Peptide Fragments / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D013927:Thrombosis / Q000209:etiology / Q000378:metabolism*
chemical_list: D001054:Apolipoproteins A; D017270:Lipoprotein(a); D008074:Lipoproteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C051928:lipoprotein-associated coagulation inhibitor; C018998:lysyl-plasminogen; D010958:Plasminogen