[Effects of all-trans retinoic acid, arsenic trioxide and daunorubicin on tissue factor expression in NB4 cells].
OBJECTIVE
To investigate the effects of all-trans retinoic acid(ATRA), arsenic trioxide(As2O3) and daunorubicin(DNR) on tissue factor(TF) expression in acute promyelocytic leukemia (APL) cell line NB4 cells.


METHODS
Procoagulant activity(PCA) of NB4 cells treated with 1 mumol/L ATRA, 1 mumol/L As2O3 or 0.2 microgram/ml DNR was detected using one-stage clotting assay, TF antigen by ELISA, and TF mRNA by RT-PCR.


RESULTS
Both ATRA and As2O3 could down-regulate the TF antigen, its mRNA transcription and membrane PCA of NB4 cells with a time-dependent manner, while DNR was shown to increase these parameters. Moreover, by dideoxy sequencing of DNA fragment derived from PCR, it was found that there was a exon 5 deletion transcript of TF in APL cells. Its biological significance remained unknown.


CONCLUSIONS
TF expression and PCA of APL cells may be down-regulated by ATRA and As2O3, therefore, As2O3 might also improve DIC-related hemorrhage of APL while inducing APL cells to apoptosis. The distinct regulation of TF and PCA on APL cells by As2O3 and DNR may at least partially contribute to their effects on APL coagulopathy through the influence on coagulant factors activation.
Guo
W
W
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Zhu
J
J
Wang
H
H
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D003630:Daunorubicin / Q000494:pharmacology*; D005169:Factor VIII / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D010087:Oxides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D012333:RNA, Messenger; D014212:Tretinoin; D005169:Factor VIII; D013925:Thromboplastin; D000077237:Arsenic Trioxide; D003630:Daunorubicin