title: Enterotoxins of Staphylococcus aureus induce a cytopathic effect in cell lines.
abstract: We describe the use of cell cultures for the detection of Staphylococcus aureus enterotoxins. Madin Darby Bovine Kidney (MDBK), Bovine Embryo lung cells (PEB) and Dog Carcinoma Cell line (A-72) cell monolayers were tested. MDBK, A-72 and PEB cell lines proved to be susceptible to the cytotoxic effect induced by staphylococcal enterotoxins. A cytopathic effect was observed in some cases after 2 hours of incubation. The PEB cell line was the most susceptible and may be used as a useful and cheap method for screening enterotoxigenic S. aureus strains.
authors.affiliation: Department of Health and Animal Welfare--Faculty of Veterinary Medicine--Valenzano (Bari) Italy.
authors.forename: G
authors.initials: G
authors.lastname: Normanno
authors.forename: G
authors.initials: G
authors.lastname: Celano
authors.forename: A
authors.initials: A
authors.lastname: Dambrosio
authors.forename: L
authors.initials: L
authors.lastname: Lassandro
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002417:Cattle; D004285:Dogs; D004768:Enterotoxins / Q000276:immunology / Q000494:pharmacology*; D007369:Interferon Inducers / Q000276:immunology / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology; D008168:Lung / Q000166:cytology; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D017186:Titrimetry; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014774:Virulence
chemical_list: D000906:Antibodies; D004768:Enterotoxins; D007369:Interferon Inducers; C049463:enterotoxin E, Staphylococcal; C019368:enterotoxin D, Staphylococcal; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal