title: Novel HLA-B alleles identified in potential marrow donors: B*3917, B*1405 and B*3528.
abstract: Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing of volunteer bone marrow donors suggested the presence of variants of known HLA-B alleles in five individuals. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Three African-American individuals had a novel HLA-B*39 allele (B*3917), and another African-American was found to have a novel HLA-B*14 allele (B*1405). In a third individual of Hispanic origin, a novel HLA-B*35 allele (B*3528) was identified. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19035, USA.
authors.forename: I S
authors.initials: IS
authors.lastname: Gourley
authors.forename: J
authors.initials: J
authors.lastname: Kearns
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Bassinger
authors.forename: G D
authors.initials: GD
authors.lastname: Montoya
authors.forename: M
authors.initials: M
authors.lastname: McKeen
authors.forename: S
authors.initials: S
authors.lastname: Birkos
authors.forename: T M
authors.initials: TM
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D014644:Genetic Variation; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008913:Minority Groups; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D014019:Tissue Donors
chemical_list: D015235:HLA-B Antigens