[Optional growth medium and conditions for mass production of Pandora delphacis mycelia in submerged culture].
The entomophthoraceous fungi are important microbial control agents for insect control but their mass production is usually difficult and expensive. To produce in large quantity the mycelia of the entomophthoraceous fungus, Pandora delphacis (isolate F95129), this study was aimed at replacing expensive components of liquid medium that is usually used in laboratory only with cheap materials easily available. Based on comparative experiments with primary components of several media designed and optional conditions for submerged culture, an appropriate medium was recognized that included(per liter) 10 g of homemade yeast extract, 10 g industrial fish meal, 10 g wheat bran, 15 g corn meal, 1.0 g KH2PO4, 3.0 g NH4NO3, and 0.25 g MgSO(4).7H2O. The optional conditions for submerged culture was 25-30 degrees C, initial pH 6.5, and 40% (V/V) of flask filled with the medium and 10% of initial inoculum (liquid culture containing mycelia) for inoculation. Using the selected medium and the conditions considered, 48 h culture resulted in a considerably high yield of dry mycelia(> 25 mg/mL) with desirable capacity of spore production.
Liu
Z Q
ZQ
Research Institute of Microbiology, Zhejiang University, Hangzhou 310029, China.
Feng
M G
MG
D003470:Culture Media; D005285:Fermentation; D005658:Fungi / Q000254:growth & development*; D025282:Mycelium / Q000254:growth & development*; D013172:Spores, Fungal / Q000254:growth & development
D003470:Culture Media