Enhancement of beta-sitosterol transformation in Mycobacterium vaccae with increased cell wall permeability.
Mycobacterium vaccae exposed to compounds which are known to disorganise the cell wall composition and architecture (protamine, glycine) showed increased specific activity in beta-sitosterol biotransformation to androstene derivatives, intennediates in the production of most medical steroids. GC/MS analysis of free lipid fatty acids revealed higher content of unsaturated compounds, mainly C16:1 and C18:1 in protamine- and glycine-treated cells than that in control cells, which seems to change the permeability features of the cell wall barrier, facilitating hydrophobic beta-sitosterol diffusion.
Korycka-Machała
M
M
Centre for Microbiology and Virology, Polish Academy of Sciences, 93-232 Lódź, Lodowa 106, Poland.
Rumijowska-Galewicz
A
A
Lisowska
K
K
Ziolkowskit
A
A
Sedlacze
L
L
D000730:Androstadienes / Q000378:metabolism; D000735:Androstenedione / Q000096:biosynthesis; D001711:Biotransformation; D002463:Cell Membrane Permeability / Q000187:drug effects; D002473:Cell Wall / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005998:Glycine / Q000494:pharmacology; D009161:Mycobacterium / Q000187:drug effects / Q000378:metabolism*; D011479:Protamines / Q000494:pharmacology; D012855:Sitosterols / Q000378:metabolism*
D000730:Androstadienes; D005227:Fatty Acids; D011479:Protamines; D012855:Sitosterols; C006573:1,4-androstadiene-3,17-dione; D000735:Androstenedione; C025473:gamma-sitosterol; D005998:Glycine