The role of dopamine transporter imaging agent [99mTc]TRODAT-1 in hemi-parkinsonism rat brain.
This study aims to investigate the relationship between the determination of dopamine level by high performance liquid chromatography (HPLC) with electrochemical detection (ECD) and the detection of dopamine transporter (DAT) counts using autoradiography with DAT image agent [99mTc]TRODAT-1. For striatal lesions, pretreatment of 6-hydroxydopamine (6-OHDA) in the medial forebrain bundle shows that autoradiogaphic labeling of striatum region is reduced to near-background level. Using HPLC with ECD, unilateral 6-OHDA treatment is associated with significant (p < 0. 0002) reductions of dopamine levels. For the striatum of the 6-OHDA-lesioned side, dopamine content and DAT counts are reduced to 97% and 90%, respectively. Thus, our observation indicates a potential of using [99mTc]TRODAT-1 for the evaluation of animal DAT.
Chen
Y K
YK
Graduate Institute of Medical Science, National Defense Medical Center, Taiwan, ROC.
Liu
R S
RS
Huang
W S
WS
Wey
S P
SP
Ting
G
G
Liu
J C
JC
Shen
Y Y
YY
Wan
F J
FJ
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D008297:Male; D015609:Organotechnetium Compounds; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000378:metabolism; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D013565:Sympatholytics / Q000494:pharmacology*; D015899:Tomography, Emission-Computed, Single-Photon; D014326:Tropanes
D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D013565:Sympatholytics; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1; D016627:Oxidopamine; D004298:Dopamine