Hypothalamo-neurohypophysial magnocellular neuroendocrine system of field voles after acute heat or cold stress.
Hypothalamo-neurohypophysial magnocellular neuroendocrine system (H-NH MC NES) of field voles was described in normal conditions and after acute heat or cold stress. 60 mature males of field voles were used for examinations after 2 weeks of adaptation to stable conditions of laboratory keeping. 10 animals were the controls. Another group of 25 field voles were subjected to sudden cold of -15 degrees C for 3 hours. The third group of 25 field voles were subjected to sudden heat of +32 degrees C for 3 hours. Neurosecretory material in paraffin 6 microns sections was stained with paraaldehyde fuchsin (fixed in Bouine fluid in the modyfication of Bock et al. 1968) and tigroid was stained with toluidine blue (fixed in Carnoy fluid). Routine staining with hematoxylin and eosin was used for comparison. It was found that H-NH MC NES in field voles has similar body structure as that described in many reports for laboratory H-NH MC NES of rodents. Supraoptic nucleus (SON) consisted of large multipolar neurocytes and paraventricular nucleus (PVN) consisted of slightly smaller neurocytes. PVN also included areas with small neurocytes. Acute heat or cold stress evoked enhanced concentration of neurosecret in SON and PVN and in the remaining H-NH MC NES structures of field voles simultaneously a decrease of tigroid contents was observed in neurocytes of SON and PVN after heat stress.
Sawicki
B
B
Department of Histology, Medical Academy of Białystok, Poland.
Borowska
H
H
Arciszewska
E
E
D000818:Animals; D003411:Arvicolinae; D003080:Cold Temperature / Q000009:adverse effects*; D018882:Heat Stress Disorders / Q000473:pathology*; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000473:pathology*; D010904:Pituitary Gland, Posterior / Q000473:pathology*; D022142:Secretory Vesicles / Q000648:ultrastructure*; D013312:Stress, Physiological / Q000209:etiology / Q000473:pathology; D013495:Supraoptic Nucleus / Q000473:pathology*