Promoted expression of mast cell-specific proteases in IgE-dependent passive cutaneous anaphylaxis responses.
BACKGROUND
Various factors can influence the protease expression phenotype of mast cells.


METHODS
In an effort to understand the potential role of the mast cell proteases in the IgE-dependent passive cutaneous anaphylaxis (PCA) responses of murine tissues, we studied the changes of proteases expression. The expressions of proteases were examined by Northern blotting and immunohistochemistry.


RESULTS
Promoted expression phenotypes of mouse mast cell protease (mMCP)-4, and rat mast cell protease I were accompanied by initiation of anti-dinitrophenyl (DNP) IgE-induced PCA responses, suggesting that the induction of these proteases expression are associated with IgE-mediated anaphylaxis responses. Elevated level of the L-histidine decarboxylase (HDC) mRNA expression was also observed in the PCA tissues and the activated mast cells, compared with that of the corresponding control tissue and cells, due to the activation of mast cells.


CONCLUSIONS
Promoted protease expression phenotype appears to be linked with the induction of HDC expression.
Park
S J
SJ
Department of Oriental Pharmacy, College of Pharmacy, Korea Institute of Oriental Pharmacy, Wonkwang University, Iksan, Chonbuk 570-749, South Korea.
Cho
S S
SS
Oh
J M
JM
Han
M K
MK
Song
Y S
YS
Bae
H R
HR
Yoo
Y H
YH
Kim
H M
HM
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D010450:Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007150:Immunohistochemistry; D008407:Mast Cells / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000166:cytology / Q000201:enzymology; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D012333:RNA, Messenger; D007073:Immunoglobulin E; D010450:Endopeptidases