title: Antigen-specific T-cell responses in cattle immunized with antigens of Tritrichomonas foetus.
abstract: The cellular immune responses of cattle immunized with antigens of Tritrichomonas foetus were investigated. Subcutaneous injections of antigen preparations primed bovine peripheral blood mononuclear cells (PBMC) by 30 days of immunization as demonstrated by antigen-specific proliferation and by cytokine production upon antigen challenge of PBMC. Antigen-specific T-cells derived from PBMC responded by production of interferon (IFN)-gamma message detected by reverse transcriptase polymerase chain reaction, secreted IFN-gamma detected by enzyme-linked immunosorbent assay, and intracellular IFN-gamma detected by flow cytometry. Phenotypic analysis of PBMC responding in vitro to parasite antigen demonstrated a shift from a mixed CD4+, CD8+, gammadelta+, to predominantly CD4+, CD8-, gammadelta- phenotype in the Tf190-primed PBMC. In conclusion, systemic immunization of cattle with parasite antigen results in priming of bovine T-cells that are antigen specific and can produce an anamnestic IFN-gamma response to subsequent stimulation with antigens of T. foetus.
authors.affiliation: Department of Veterinary Molecular Biology, Montana State University, Bozeman 59717, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Voyich
authors.forename: A
authors.initials: A
authors.lastname: Palecanda
authors.forename: D E
authors.initials: DE
authors.lastname: Burgess
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D002448:Cell Adhesion; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007114:Immunization / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D009000:Monocytes; D011247:Pregnancy; D011528:Protozoan Infections / Q000276:immunology / Q000469:parasitology; D011529:Protozoan Infections, Animal; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D016835:Tritrichomonas foetus / Q000254:growth & development / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D000953:Antigens, Protozoan; D007371:Interferon-gamma