[Heparin enhances the stimulating effect of thrombopoietin on megakaryocytopoiesis].
OBJECTIVE
To verify whether heparin could enhance the stimulating effect of Tpo on megakaryocytopoiesis.


METHODS
Megakaryocytic leukemia cell line M07e, human cord blood CD34+ cells and BALB/c mice were used in this study. The effects of heparin and/or Tpo on megakaryocytopoiesis were studied by [3H]-TdR incorporation assay, CFU-MK plasmic semi-solid culture and in vivo experiment in BALB/c mice.


RESULTS
Heparin could enhance the stimulating effect of Tpo on stimulating the proliferation of M-07e, the growth of CFU-MK from human cord blood CD34+ cells and in vivo megakaryocytopoiesis in mice.


CONCLUSIONS
Heparin participates in positive regulation of megakaryocytopoiesis by enhancing the megakaryocytopoietic activities of Tpo.
Chen
Q
Q
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Shen
Z
Z
Han
Z
Z
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013926:Thrombopoietin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006493:Heparin; D013926:Thrombopoietin