New molecular marker for Trypanosoma (Duttonella) vivax identification.
Trypanosoma vivax is a widespread hemoparasite in tropical areas and is pathogenic to ruminant domestic livestock as well as wild ruminants. The accurate identification of parasites in both hosts and vectors is crucial for epidemiological studies and disease control programs. We describe here the development of molecular markers specific for T. vivax identification. These markers were used to identify mouthpart infections in field-collected tsetse flies from Cameroon. The markers target the genomic sequence of a species-specific antigen from the bloodstream stages. No cross amplification with other trypanosome species was observed, which makes the markers a reliable tool to detect T. vivax infections, both in hosts and vectors. The PCR-amplified sequence contains a (CA)(n) microsatellite repeat for which 11 different alleles were identified. This microsatellite, which showed high polymorphism, provides a suitable marker for population genetic studies.
Morlais
I
I
Laboratoire d'Epidémiologie des Maladies à Vecteurs, IRD, BP 5045, 34032 Montpellier, France. isabelle.morlais.1@nd.edu
Ravel
S
S
Grébaut
P
P
Dumas
V
V
Cuny
G
G
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D013045:Species Specificity; D016689:Trypanosoma vivax / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014370:Tsetse Flies / Q000469:parasitology*
D016054:DNA, Protozoan; D005819:Genetic Markers