Cloning and sequencing of the fragment of angiostatin K (1-3) gene.
OBJECTIVE
To secure anti-endotheliocyte hyperplasia functional fragment of human angiostatin K (1-3) gene.


METHODS
As the template of leukocytotic cDNA, functional fragment of human angiostatin K (1-3) cDNA was amplified by PCR, cloned into the vector pGEM-3Zf, and sequenced according to Dye primer sequencing kit.


RESULTS
The functional fragment of angiostatin K (1-3) cDNA (859 bp) was obtained by PCR and determined by sequencing.


CONCLUSIONS
The angiostatin K (1-3) gene has been cloned. The gene is of great significance in tumor-antiangiogenesis therapy.
Zhang
X
X
Institute of Neurosurgery of PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Wu
J
J
Gao
D
D
Chai
Y
Y
Fei
Z
Z
Yi
L
L
Liu
X
X
Liang
J
J
D020533:Angiogenesis Inhibitors / Q000235:genetics*; D043168:Angiostatins; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics*; D010958:Plasminogen / Q000235:genetics*; D017422:Sequence Analysis, DNA
D020533:Angiogenesis Inhibitors; D010446:Peptide Fragments; D043168:Angiostatins; D010958:Plasminogen; D004247:DNA