title: Amino acid-induced translation of TOP mRNAs is fully dependent on phosphatidylinositol 3-kinase-mediated signaling, is partially inhibited by rapamycin, and is independent of S6K1 and rpS6 phosphorylation.
abstract: Vertebrate TOP mRNAs contain an oligopyrimidine tract at their 5' termini (5'TOP) and encode components of the translational machinery. Previously it has been shown that they are subject to selective translational repression upon growth arrest and that their translational behavior correlates with the activity of S6K1. We now show that the translation of TOP mRNAs is rapidly repressed by amino acid withdrawal and that this nutritional control depends strictly on the integrity of the 5'TOP motif. However, neither phosphorylation of ribosomal protein (rp) S6 nor activation of S6K1 per se is sufficient to relieve the translational repression of TOP mRNAs in amino acid-starved cells. Likewise, inhibition of S6K1 activity and rpS6 phosphorylation by overexpression of dominant-negative S6K1 mutants failed to suppress the translational activation of TOP mRNAs in amino acid-refed cells. Furthermore, TOP mRNAs were translationally regulated by amino acid sufficiency in embryonic stem cells lacking both alleles of the S6K1 gene. Inhibition of mTOR by rapamycin led to fast and complete repression of S6K1, as judged by rpS6 phosphorylation, but to only partial and delayed repression of translational activation of TOP mRNAs. In contrast, interference in the phosphatidylinositol 3-kinase (PI3-kinase)-mediated pathway by chemical or genetic manipulations blocked rapidly and completely the translational activation of TOP mRNAs. It appears, therefore, that translational regulation of TOP mRNAs, at least by amino acids, (i) is fully dependent on PI3-kinase, (ii) is partially sensitive to rapamycin, and (iii) requires neither S6K1 activity nor rpS6 phosphorylation.
authors.affiliation: Department of Biochemistry, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Tang
authors.forename: E
authors.initials: E
authors.lastname: Hornstein
authors.forename: M
authors.initials: M
authors.lastname: Stolovich
authors.forename: G
authors.initials: G
authors.lastname: Levy
authors.forename: M
authors.initials: M
authors.lastname: Livingstone
authors.forename: D
authors.initials: D
authors.lastname: Templeton
authors.forename: J
authors.initials: J
authors.lastname: Avruch
authors.forename: O
authors.initials: O
authors.lastname: Meyuhas
mesh_terms: D000483:Alleles; D020816:Amino Acid Motifs; D000596:Amino Acids / Q000737:chemistry*; D000935:Antifungal Agents / Q000494:pharmacology; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005799:Genes, Dominant; D006801:Humans; D008387:Marine Toxins; D008954:Models, Biological; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011743:Pyrimidines / Q000737:chemistry*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins / Q000378:metabolism*; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology*; D013997:Time Factors; D014162:Transfection
chemical_list: D000596:Amino Acids; D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D010080:Oxazoles; D019869:Phosphatidylinositol 3-Kinases; D011743:Pyrimidines; D012313:RNA; D012333:RNA, Messenger; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D020123:Sirolimus; C059041:calyculin A