title: Herpes simplex virus ophthalmic disease induced using two different methods of mice inoculation.
abstract: Two different procedures for inoculation of HSV on corneas of BALB/c mice were evaluated. The first was by the use of HSV suspensions directly on the corneas and the other was after corneal scarification. Animals by this later method presented greater morbidity and mortality than those of first group, suggesting that inoculation of HSV without scarification of the cornea should be the method of choice for the study of HSV ophthalmic infection. This model showed also be an efficient experimental system to testing antiviral drugs.
authors.affiliation: Laboratory of Viral Pathogenesis and Diagnosis, Department of Virology, Professor Paulo de Góes Institute of Microbiology, Center for Health Sciences, Federal University of Rio de Janeiro, Brazil. silviareginafgp@ig.com.br
authors.forename: S R
authors.initials: SR
authors.lastname: Pereira
authors.forename: M A
authors.initials: MA
authors.lastname: Guimarães
authors.forename: L V
authors.initials: LV
authors.lastname: Neto
authors.forename: D
authors.initials: D
authors.lastname: Segenreich
authors.forename: R B
authors.initials: RB
authors.lastname: Varella
authors.forename: V L
authors.initials: VL
authors.lastname: Antunes Chagas
authors.forename: F P
authors.initials: FP
authors.lastname: Câmara
mesh_terms: D000818:Animals; D003315:Cornea / Q000821:virology; D004195:Disease Models, Animal; D016849:Keratitis, Herpetic / Q000473:pathology / Q000821:virology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000472:pathogenicity*