Identification of F. o. cucumerinum and F. o. luffae by RAPD-generated DNA probes.
OBJECTIVE
To create a fast, sensitive and specific method for identifying Fusarium oxysporum f. sp. cucumerinum and F. o. luffae.


RESULTS
Specific DNA bands were selected as probes from RAPD profiles of 13 formae speciales of F. oxysporum. The forma specialis-specific probe OPC18300c and OPC18520f could be used to identify F. o. cucumerinum and F. o. luffae by RAPD-PCR followed dot blot hybridization, respectively.


CONCLUSIONS
A specific method for identifying F. o. cucumerinum and F. o. luffae was achieved.


CONCLUSIONS
F. oxysporum formae speciales identification with a DNA probe can be relatively rapid and provides a method to identify the pathogen without host inoculation tests.
Wang
P H
PH
Department of Microbiology, Soochow University, Taipei, Taiwan, R.O.C. phwang@mail.scu.edu.tw
Lo
H S
HS
Yeh
Y
Y
D015373:Bacterial Typing Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005670:Fusarium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D011110:Polymorphism, Genetic / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial