title: A dominant antigenic region of the hantaan virus nucleocapsid protein is located within a amino-terminal short stretch of hydrophilic residues.
abstract: The nucleocapsid (N) protein of the Hantaan virus (HTNV) is a major viral antigen that induces a strong antibody response during the acute phase of infection. By immunoblot analyses of the recombinant N proteins using human sera of the hemorrhagic fever with renal syndrome (HFRS), we have confirmed previous finding by other investigators of the presence of a highly antigenic region near the amino terminus of the HTNV N protein. We have further located the antigenic region within a short stretch of hydrophilic sequences between the 26 and the 46th amino acid residues. The recombinant glutathione S-transferase fusion proteins containing this region was expressed as a soluble form in a large quantity in Escherichia coli, and purified by a single-step affinity chromatography. The recombinant antigen also showed a similar, but a weaker reactivity with human antisera to Seoul virus (SEOV), the virus most closely related to HTNV.
authors.affiliation: Graduate School of Life Science and Biotechnology, Korea University, Seoul.
authors.forename: J I
authors.initials: JI
authors.lastname: Kang
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.forename: K
authors.initials: K
authors.lastname: Ahn
authors.forename: J W
authors.initials: JW
authors.lastname: Song
authors.forename: K J
authors.initials: KJ
authors.lastname: Song
authors.forename: L J
authors.initials: LJ
authors.lastname: Baek
authors.forename: P W
authors.initials: PW
authors.lastname: Lee
authors.forename: B Y
authors.initials: BY
authors.lastname: Ahn
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry*; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry* / Q000276:immunology; D036022:Capsid Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus