[Expressions of the metastasis-associated factors of a new human hepatocellular carcinoma cell line with highly metastatic potential].
OBJECTIVE
To explore the expressions of some metastasis-related factors in a new human hepatocellular carcinoma cell line with a highly metastatic potential by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.


METHODS
A human hepatocellular carcinoma (HCC) cell line (MHCC97) was established derived from subcutaneous xenograft of a metastatic model of human HCC in nude mice (LCI-D20) by means of alternating cell culture in vitro and grown in nude mice. RT-PCR and immunohistochemistry were performed on this line and its intrahepatic xenografts and metastatic lesions to explore the expressions of metastasis-related factors.


RESULTS
The tumorigenicity and metastatic rate to lungs of MHCC97 cell line was 100% by orthotopic inoculation. RT-PCR products for integrin alpha 5 and beta 1, uPAR, VEGF and nm23-H1 mRNA from MHCC97 cell line were positive. Immunostaining showed strongly positive for c-Met, uPAR in both of xenografts and lung metastatic lesions, and also positive in xenografts, but negative in lung metastatic lesions for integrin alpha 5 and beta 1. E-cadherin was not expressed either in xenografts or in the metastatic lesions. The PCR productions for HBsAg and HBxAg were positive, and negative for HBcAg.


CONCLUSIONS
MHCC97 cell line has a ability to express some metastasis-associated factors. DNA for virus hepatitis B may be integrated into the genome of MHCC97 cells.
Tian
J
J
Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China.
Tang
Z
Z
Xue
Q
Q
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D055293:Receptors, Urokinase Plasminogen Activator; D014157:Transcription Factors / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D015820:Cadherins; D054778:NM23 Nucleoside Diphosphate Kinases; C524898:PLAUR protein, human; C524899:Plaur protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D014157:Transcription Factors; D019859:Proto-Oncogene Proteins c-met; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins