title: A comparison of three site-directed mutagenesis kits.
abstract: In this comparative study, three different mutagenesis kits, namely the MutaGene phagemid in vitro mutagenesis kit (Bio-Rad), the Transformerä Site-Directed mutagenesis kit (Clontech) and the Quik-change site-directed mutagenesis kit (Stratagene) were used for the mutagenesis of IPNS genes. However, a large difference in mutation efficiencies among these kits was encountered. Furthermore, these kits employ different strategies with its own individual strengths and weaknesses. Thus, a comparison among these three kits to evaluate their usefulness and improvements on the strategy adopted by the Quik-change site-directed mutagenesis kit, which was the kit of choice for our work, are presented for the benefit of research work.
authors.affiliation: Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
authors.forename: P
authors.initials: P
authors.lastname: Loke
authors.forename: T S
authors.initials: TS
authors.lastname: Sim
mesh_terms: D020137:Base Pair Mismatch; D004247:DNA / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA