title: Gene frequencies of human neutrophil antigens in the Tunisian blood donors and Berbers.
abstract: Human neutrophil antigens play an important role in provoking immune neutropenia and transfusion-reactions. The aim of this study was to determine granulocyte-specific antigens on the neutrophil Fc gamma receptor IIIb (Fc gamma RIIIb, CD16b), namely, the HNA-1a(NA1) and HNA-1b(NA2) antigens and their gene frequencies in Tunisian blood donors and Berbers. One hundred and ninety-nine unrelated healthy Tunisian blood donors and Berbers were typed for HNA-1a and HNA-1b(NA1 and NA2), using polymerase chain reaction with sequence-specific primers (PCR-SSP). In 24 granulocyte samples, the HNA-1a and HNA-1b phenotypes was additionally determined by the granulocyte immunofluorescence test (GIFT) and correlated with the genotyping results. A subsequent analysis of the genotyping study showed that, the HNA-1a and HNA-1b gene frequencies observed, were 0.342 and 0.658 for Berbers, and 0.311 and 0.668 for blood donors, respectively. In the genotyping study conducted, it was determined that the HNA-1a and HNA-1b gene frequencies observed in Tunisian blood donors and Berbers are similar to those previously reported in other white populations.
authors.affiliation: National Blood Transfusion Center, Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Abid
authors.forename: M
authors.initials: M
authors.lastname: Zili
authors.forename: L
authors.initials: L
authors.lastname: Bouzid
authors.forename: R
authors.initials: R
authors.lastname: Kibech
authors.forename: N
authors.initials: N
authors.lastname: Foudhaili
authors.forename: K
authors.initials: K
authors.lastname: Joudi
authors.forename: Z
authors.initials: Z
authors.lastname: Ren Regaya
authors.forename: B
authors.initials: B
authors.lastname: Abdennaji
authors.forename: R
authors.initials: R
authors.lastname: Mrad
authors.forename: K
authors.initials: K
authors.lastname: Boukef
mesh_terms: D001782:Blood Donors; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007519:Isoantigens / Q000235:genetics*; D009504:Neutrophils / Q000276:immunology*; D014416:Tunisia
chemical_list: D007519:Isoantigens; C067704:neutrophil-specific antigen NA1, human; C072753:neutrophil-specific antigen NA2