title: Effects of low dose radiation on signal transduction of neurons in mouse hypothalamus.
abstract: OBJECTIVE
  Effects of low dose radiation on signal transduction of neurons in mouse hypothalamus were investigated.
  
  
  METHODS
  In the present study competitive protein binding assay, radioimmunoassay, in situ hybridization and immunohistochemistry were used to observe the effects of whole-body irradiation with 75 mGy X-rays on the contents of cAMP and cGMP and the expressions of c-fos mRNA, Fos protein and proopiomelanocortin (POMC) mRNA in the neurons of mouse hypothalamus.
  
  
  RESULTS
  The results showed that cAMP content in mouse hypothalamus immediately increased significantly and reached the peak value in 15 min after irradiation, and then returned to near sham-irradiation level 1 h after irradiation, followed by a small fluctuation of increase and decrease; the changes of cGMP content were basically opposite to those of cAMP content, while the changes of cAMP/cGMP ratio were basically consistent with those of cAMP content. The expression of c-fos mRNA in the neurons of hypothalamus appeared 15 min after irradiation, reached its peak value within 1 h, began to abate 2 h with its total disappearance 8 h after irradiation; the expression of Fos protein reached its peak value 8 h after irradiation, and then gradually returned to sham-irradiation level 48 h after irradiation; the expression of POMC mRNA decreased significantly 1 h after irradiation and remained at a lower level in the observation period of 12 h.
  
  
  CONCLUSIONS
  These findings implicate that low dose radiation may potentiate the activity of the neurons in mouse hypothalamus, expedite their signal transduction, and down-regulate the functions of hypothalamus-pituitary-adrenocortical axis.
authors.affiliation: MH Radiobiology Research Unit, Jilin University, Changchun 130021, China.
authors.forename: H
authors.initials: H
authors.lastname: Wan
authors.forename: S L
authors.initials: SL
authors.lastname: Gong
authors.forename: S Z
authors.initials: SZ
authors.lastname: Liu
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D015536:Down-Regulation; D005786:Gene Expression Regulation / Q000528:radiation effects; D016762:Genes, fos; D007031:Hypothalamus / Q000502:physiology / Q000528:radiation effects*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009474:Neurons / Q000502:physiology / Q000528:radiation effects*; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D011832:Radiation Injuries / Q000503:physiopathology*; D015398:Signal Transduction / Q000528:radiation effects*
chemical_list: D012333:RNA, Messenger; D011333:Pro-Opiomelanocortin; D000242:Cyclic AMP; D006152:Cyclic GMP