Identification of BV/ODV-C42, an Autographa californica nucleopolyhedrovirus orf101-encoded structural protein detected in infected-cell complexes with ODV-EC27 and p78/83.
orf101 is a late gene of Autographa californica nucleopolyhedrovirus (AcMNPV). It encodes a protein of 42 kDa which is a component of the nucleocapsid of budded virus (BV) and occlusion-derived virus (ODV). To reflect this viral localization, the product of orf101 was named BV/ODV-C42 (C42). C42 is predominantly detected within the infected-cell nucleus: at 24 h postinfection (p.i.), it is coincident with the virogenic stroma, but by 72 h p.i., the stroma is minimally labeled while C42 is more uniformly located throughout the nucleus. Yeast two-hybrid screens indicate that C42 is capable of directly interacting with the viral proteins p78/83 (1629K) and ODV-EC27 (orf144). These interactions were confirmed using blue native gels and Western blot analyses. At 28 h p.i., C42 and p78/83 are detected in two complexes: one at approximately 180 kDa and a high-molecular-mass complex (500 to 600 kDa) which also contains EC27.
Braunagel
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Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843-2475, USA.
Guidry
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Rosas-Acosta
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Engelking
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Summers
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry* / Q000235:genetics; D018411:Spodoptera; D015678:Viral Structural Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
C101553:Ac-odv-ec27 protein, Autographa californica nucleopolyhedrovirus; D036022:Capsid Proteins; D015678:Viral Structural Proteins