The use of P-aminobenzoic acid and chromic oxide to confirm complete excreta collection in a carnivore, Felis silvestris catus.
Complete excreta collection is a pre-requisite for several protocols in protein metabolism, and lack of confidence in achieving this may be increased when working with carnivores. Recovery of p-aminobenzoic acid (PABA) as a check for complete urine collection and chromic oxide for complete faeces collection were assessed in the cat. A single oral dose of PABA (4 mg/kg BW) was excreted more slowly than has been reported in the human (82% recovery at 6 h). A daily dose of PABA proved a useful method for confirming complete urine collection in the cat, and was 99% excreted in 72 h. Chromic oxide (500 mg/cat) was administered orally and recovery of chromium in the faeces was 90% after 96 h. A HPLC method for the analysis of PABA in cat urine was developed, and from the application of the techniques to a nitrogen balance study, it was concluded that PABA and chromic oxide are useful checks for complete excreta collection in the cat.
Russell
K
K
Waltham Centre for Pet Nutrition, Freeby Lane, Leicestershire LE14 4RT, Melton Mowbray, UK. kim.russell@eu.effem.com
Craig
I D
ID
Rawlings
J M
JM
Millward
D J
DJ
Harper
E J
EJ
D010129:4-Aminobenzoic Acid / Q000008:administration & dosage / Q000652:urine*; D000284:Administration, Oral; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000652:urine; D001835:Body Weight; D002214:Capsules / Q000008:administration & dosage; D002415:Cats / Q000378:metabolism* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D002857:Chromium / Q000032:analysis; D017608:Chromium Compounds / Q000008:administration & dosage / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005260:Female; D005612:Freeze Drying; D005772:Gastrointestinal Transit; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D015203:Reproducibility of Results; D013048:Specimen Handling; D013997:Time Factors
D015415:Biomarkers; D002214:Capsules; D017608:Chromium Compounds; D002857:Chromium; D009584:Nitrogen; D010129:4-Aminobenzoic Acid; C023600:chromic oxide