Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA microarray analysis.
The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.
Fukusaki
E
E
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan. fukusaki@bio.eng.osaka-u.ac.jp
Oishi
T
T
Tanaka
H
H
Kajiyama
S
S
Kobayashi
A
A
D003001:Cloning, Molecular; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D017239:Paclitaxel / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D017239:Paclitaxel