Detection of maternal DNA in umbilical cord plasma by fluorescent PCR amplification of short tandem repeat sequences.
Recently, maternal DNA was detected in umbilical cord blood using PCR amplification of minisatellite sequences. The presence of maternal DNA was demonstrated in 1% to 100% of umbilical cord blood samples. The objective of this study was to determine the frequency of cord blood contamination by maternal genetic material. We used fluorescent PCR amplification of highly polymorphic short tandem repeat (STR) markers to detect maternal DNA in umbilical cord plasma.
Bauer
M
M
Department of Obstetrics and Gynecology, Karl Franzens University of Graz, Austria.
Orescovic
I
I
Schoell
W M
WM
Bianchi
D W
DW
Pertl
B
B
D004247:DNA / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D005453:Fluorescence; D005819:Genetic Markers; D006801:Humans; D009035:Mothers; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D020080:Tandem Repeat Sequences
D005819:Genetic Markers; D004247:DNA