title: Production of gamma-linolenic acid by Mortierella isabellina grown on hexadecanol.
abstract: OBJECTIVE
  To optimize the production of linolenic acid by Mortierella isabellina grown on hexadecanol.
  
  
  RESULTS
  Effects of culture conditions such as culture time, pH of medium, hexadecanol concentration, incubation temperature and ageing of mycelia on production of linolenic acid were studied. The production of gamma-linolenic acid reached 2.44 mg ml-1 (271 mg g-1 dry cells) when Mortierella isabellina was cultivated in a medium consisting of 2% hexadecanol and 1% yeast extract at 23 degrees C for 120 h and then the mycelia, after removal of medium by suction filtration, were allowed to stand for a further 15 d at 5 degrees C.
  
  
  CONCLUSIONS
  Ageing of mycelia and incubation temperature showed predominant effects on the increased linolenic acid production.
  
  
  CONCLUSIONS
  This study highlights effective conditions for increasing linolenic acid production by Mortierella isabellina grown on hexadecanol.
authors.affiliation: Department of Chemistry, Jilin University, Jiefang Road 119#, Changchun 130023, China. ctx@mail.jlu.edu.cn
authors.forename: M
authors.initials: M
authors.lastname: Xian
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: Y
authors.initials: Y
authors.lastname: Kang
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Bi
authors.forename: K
authors.initials: K
authors.lastname: Zhen
mesh_terms: D003470:Culture Media / Q000737:chemistry; D005233:Fatty Alcohols / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D020094:Mortierella / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature; D017962:alpha-Linolenic Acid / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D005233:Fatty Alcohols; D017962:alpha-Linolenic Acid