title: Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels.
abstract: We previously applied intravital lectin perfusion in mouse models to elucidate mechanisms underlying vascular permeability. The present work transfers this technique to human models, analysing vascular permeability in macro- and microvessels. Human vascular endothelial surface carbohydrate biochemistry differs significantly from its murine counterpart, lacking alpha-galactosyl epitopes and expressing the L-fucose moiety in the glycocalyx; the poly-N-lactosamine glycan backbone is common to all mammals. We examined extensively lectin binding specificities in sections and in vivo, and then applied the poly-N-lactosamine-specific lectin LEA and the L-fucose-specific lectin UEA-I in human intravital perfusions. Transendothelial transport differed in macrovessels and microvessels. In microvessels of adult human fat tissue, rectal wall and rectal carcinomas, slow transendothelial transport by vesicles was followed by significant retention at the subendothelial basement membrane; paracellular passage was not observed. Passage time exceeded 1 h. Thus we found barrier mechanisms resembling those we described previously in murine tissues. In both adult and fetal macrovessels, the vena saphena magna and the umbilical vein, respectively, rapid passage across the endothelial lining was observed, the tracer localising completely in the subendothelial tissues within 15 min; vesicular transport was more rapid than in microvessels, and retention at the subendothelial basement membrane briefer.
authors.affiliation: Institute of Anatomy and Histology, University of Innsbruck, Müllerstrasse 59, 6020 Innsbruck, Austria. Paul.Debbage@uibk.ac.at
authors.forename: P L
authors.initials: PL
authors.lastname: Debbage
authors.forename: E
authors.initials: E
authors.lastname: Sölder
authors.forename: S
authors.initials: S
authors.lastname: Seidl
authors.forename: P
authors.initials: P
authors.lastname: Hutzler
authors.forename: B
authors.initials: B
authors.lastname: Hugl
authors.forename: D
authors.initials: D
authors.lastname: Ofner
authors.forename: A
authors.initials: A
authors.lastname: Kreczy
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply; D001692:Biological Transport; D001808:Blood Vessels / Q000378:metabolism* / Q000648:ultrastructure; D002199:Capillary Permeability / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D005169:Factor VIII / Q000032:analysis; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D008854:Microscopy, Electron; D010477:Perfusion; D037121:Plant Lectins; D012007:Rectum / Q000098:blood supply / Q000737:chemistry; D012501:Saphenous Vein / Q000378:metabolism / Q000648:ultrastructure; D014470:Umbilical Cord / Q000098:blood supply; D014471:Umbilical Veins / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C034981:tomato lectin; D005169:Factor VIII; D016650:Fluorescein-5-isothiocyanate