title: Histochemical differentiation of Dirofilaria immitis, Dirofilaria repens and Acanthocheilonema dracunculoides microfilariae by staining with a commercial kit, Leucognost-SP.
abstract: The diagnosis of canine heartworm infection is based upon the presence of circulating Dirofilaria immitis microfilariae or on techniques for the detection of serum antibodies or antigens. In the first of these, discrimination between D. immitis, D. repens and Acanthocheilonema dracunculoides microfilariae is based upon the acid phosphatase histochemical stain. In this paper, we propose an alternative technique for histochemical staining using a commercial kit test of naphthol-AS-OL (Leucognost-SP). This offers the advantages of speed and simplicity as compared to the standard Barka procedure.
authors.affiliation: Departamento de Patología Animal, Facultad de Veterinaria de Zaragoza, Universidad de Zaragoza, C/Miguel Servet, 177, 50013-Zaragoza, Spain. mperilop@posta.unizar.es
authors.forename: M A
authors.initials: MA
authors.lastname: Peribáñez
authors.forename: J
authors.initials: J
authors.lastname: Lucientes
authors.forename: S
authors.initials: S
authors.lastname: Arce
authors.forename: M
authors.initials: M
authors.lastname: Morales
authors.forename: J A
authors.initials: JA
authors.lastname: Castillo
authors.forename: M J
authors.initials: MJ
authors.lastname: Gracia
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D003937:Diagnosis, Differential; D004153:Dipetalonema / Q000145:classification* / Q000201:enzymology; D004154:Dipetalonema Infections / Q000175:diagnosis / Q000662:veterinary*; D004182:Dirofilaria / Q000145:classification* / Q000201:enzymology; D004183:Dirofilaria immitis / Q000145:classification* / Q000201:enzymology; D004184:Dirofilariasis / Q000175:diagnosis*; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D006651:Histocytochemistry / Q000379:methods / Q000662:veterinary; D008842:Microfilariae / Q000145:classification / Q000201:enzymology; D011933:Reagent Kits, Diagnostic / Q000662:veterinary
chemical_list: D011933:Reagent Kits, Diagnostic; D000135:Acid Phosphatase