Using 2', 7'-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents.
Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.
Bland
E J
EJ
Fungal Biotechnology Group, School of Biosciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK.
Keshavarz
T
T
Bucke
C
C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D004396:Coloring Agents / Q000494:pharmacology*; D005452:Fluoresceins / Q000494:pharmacology*; D006801:Humans; D008956:Models, Chemical; D009504:Neutrophils / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry*; D017382:Reactive Oxygen Species; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013997:Time Factors
C110400:2',7'-dichlorodihydrofluorescein diacetate; D000276:Adjuvants, Immunologic; D004396:Coloring Agents; D005452:Fluoresceins; D011134:Polysaccharides; D017382:Reactive Oxygen Species