title: HLA-DR expression in B-lymphocytes in vitro is not suppressed by the absence of exogenous antigens.
abstract: The proper loading of exogenous peptide antigens affects the transport and cell surface expression of MHC class II molecules. In the present study, the goal was to determine to what extent this step determines the cell surface expression level of MHC class II molecules, such as the HLA-DR. EBV-transformed B-cells, were cultured either in a serum- and protein-free medium, or in a medium that contained different concentrations of exogenous antigens. Using HLA-DR-specific antibodies, the induction of the MHC class II expression was observed in cells that were cultured under serum-and protein-free conditions, when compared to those cultured with exogenous protein antigens. This upregulation was completely suppressed to the normal level by the addition of a high concentration of hen egg lysozyme to the serum- and protein-free medium. This indicates that exogenous proteins regulate the HLA-DR expression. To further examine whether this modulation is controlled at the transcription level, the expression of the HLA-DR beta-chain mRNA was analyzed by reverse transcription-PCR and Northern blots. The same levels of HLA-DRB mRNA were detectable in both culture conditions, indicating that the present observation is dependent on some regulatory mechanisms at the post-transcriptional level. This might include a different pathway for trafficking of HLA-DR molecules to the cell surface, since peptide-binding assays revealed that a high proportion of cell surface HLA-DR molecules under the serum- and protein-free condition were transported to the cell surface without associated peptide antigens.
authors.affiliation: Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon.
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Na
authors.forename: E W
authors.initials: EW
authors.lastname: Cho
authors.forename: K L
authors.initials: KL
authors.lastname: Kim
mesh_terms: D000941:Antigens / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000276:immunology; D003470:Culture Media; D015870:Gene Expression; D006684:HLA-DR Antigens / Q000235:genetics* / Q000378:metabolism; D004854:Herpesvirus 4, Human; D006801:Humans; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D000941:Antigens; D003470:Culture Media; D006684:HLA-DR Antigens; D010455:Peptides; D012333:RNA, Messenger