title: Further evaluation of an in vivo micronucleus test on rat and mouse skin: results with five skin carcinogens.
abstract: In a previous paper, we presented a practical in vivo micronucleus (MN) test that used rat skin as the target organ. To evaluate the test, as well as to determine the reproducibility and applicability of the method to mice, we used it to test the effect of five skin carcinogens (N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline 1-oxide (4NQO), 7,12-dimethylbenz[a]anthracene (DMBA), and benzo[a]pyrene (B[a]P)) on rat and mouse skin. All five compounds significantly and dose-dependently increased the MN frequencies in the basal cells of the chemical-treated skin. These results indicated the reproducibility of the test results and also the applicability of the test to mice as well as rats.
authors.affiliation: Human Safety Evaluation Center, Lion, 100 Tajima, Odawara-shi, Kanagawa 256-0811, Japan. ntaka@lion.co.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Haresaku
authors.forename: Akira
authors.initials: A
authors.lastname: Fukushima
authors.forename: Tsuneaki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Adachi
authors.forename: Mitsuteru
authors.initials: M
authors.lastname: Masuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects*
chemical_list: D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; C013525:ENNG; D015112:4-Nitroquinoline-1-oxide; D015127:9,10-Dimethyl-1,2-benzanthracene