title: Structural analyses of the lipopolysaccharides from Chlamydophila psittaci strain 6BC and Chlamydophila pneumoniae strain Kajaani 6.
abstract: Lipopolysaccharides (LPSs) of Chlamydophila psittaci 6BC and Chlamydophila pneumoniae Kajaani 6 contain 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), GlcN, organic bound phosphate, and fatty acids in the molar ratios of approximately 3:2:2.2:4.8 and approximately 2.9:2:2.1:4.9, respectively. The LPSs were immunoreactive with a monoclonal antibody against a family-specific epitope of chlamydial LPS. This finding, together with methylation analyses of both LPSs and MALDI-TOF MS experiments on de-O-, and de-O,N-acylated LPSs, indicate the presence of a Kdo trisaccharide proximal to lipid A having a structure alpha-Kdo-(2-->8)-alpha-Kdo-(2-->4)-alpha-Kdo, which appears to be the main component of the core region in the native chlamydial LPSs. In the de-O-acylated LPSs from Chl. psittaci 6BC and Chl. pneumoniae Kajaani 6, two major molecular species are present that differ in distribution of amide-bound hydroxy fatty acids over both GlcN. It appears that either two (R)-3-hydroxy-18-methylicosanoic acids or one (R)-3-hydroxy-18-methylicosanoic acid and one (R)-3-hydroxyicosanoic acid are attached to the GlcN residues. In contrast, the de-O-acylated LPS of Chl. psittaci PK 5082 contains one major molecular species that has two (R)-3-hydroxyicosanoic acid residues attached to two GlcN residues.
authors.affiliation: Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 45 Bratislava, Slovak Republic.
authors.forename: A
authors.initials: A
authors.lastname: Hussein
authors.forename: L
authors.initials: L
authors.lastname: Skultéty
authors.forename: R
authors.initials: R
authors.lastname: Toman
mesh_terms: D015153:Blotting, Western; D002240:Carbohydrate Sequence; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D002691:Chlamydophila psittaci / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010755:Organophosphates / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013400:Sugar Acids / Q000032:analysis
chemical_list: D005227:Fatty Acids; D008070:Lipopolysaccharides; D010755:Organophosphates; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate