[Expression and role of cyclin D3 in childhood acute leukemia].
OBJECTIVE
To investigate the expression and role of cyclin D in childhood acute leukemia(AL).


METHODS
Immunohistochemistry was used to detect the expression of cyclin D1, D2, D3 in 43 samples of childhood AL patients and three leukemic cell lines. Cyclin D3 antisense oligodeoxynucleotides were used in in vitro culture study.


RESULTS
Cyclin D3 expression was positive in 47% (14/30) of the childhood acute lymphoblastic leukemia and 38% (5/13) of the acute myelogenous leukemia patients and in CEM cells. Cyclin D1 was overexpressed in 5% (2/43) of the AL patients. No overexpression of cyclin D2 was detected. Incubation of CEM cells with cyclin D3 antisense oligodeoxynucleotides resulted in inhibition of cell proliferation.


CONCLUSIONS
Cyclin D3 was overexpressed in childhood AL and it played an important role in the proliferation of leukemic cells.
Zhang
L
L
Department of Pediatrics, People's Hospital, Beijing Medical University, Beijing 100044.
Li
J
J
D000293:Adolescent; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D019938:Cyclin D1 / Q000097:blood; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins / Q000097:blood*; D006801:Humans; D007223:Infant; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000473:pathology; D014407:Tumor Cells, Cultured
C080336:CCND2 protein, human; C534696:CCND3 protein, human; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins; D019938:Cyclin D1