title: Progesterone stimulates the expression of aminopeptidase A/angiotensinase in human choriocarcinoma cells.
abstract: In human placenta aminopeptidase A (APA), a principal enzyme that converts angiotensin II to angiotensin III, seems to be involved in angiotensin II metabolism during pregnancy. In this study, we investigated the possible effects of progesterone and estrogen on APA mRNA and protein levels in choriocarcinoma cells as a model for placenta. By RNase protection assay, progesterone induced higher APA mRNA levels than estrogen at the same concentration. Progesterone exhibited dose-dependent stimulation of APA mRNA, 1.8-fold increase at 10(-6) m for 24 h treatment. Progesterone at 10(-6) m increased APA mRNA levels within 12 h and in time-dependent fashion up to 24 h. Fluorescence-activated cell sorting analysis and measurements of APA activities revealed the induction of APA protein by progesterone. Expression of progesterone receptors (PR) and glucocorticoid receptors (GR) were determined in these cells by RT-PCR, which suggested that the progesterone's actions might be displayed through PR and/or GR. These findings may serve as a useful model to study the effects of progesterone on angiotensin II metabolism in placenta, although the physiological validity of these studies remains to be clarified.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya 466-8550, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Katsumata
authors.forename: S
authors.initials: S
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Ino
authors.forename: K
authors.initials: K
authors.lastname: Iwanaga
authors.forename: N
authors.initials: N
authors.lastname: Kurosawa
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: F
authors.initials: F
authors.lastname: Kikkawa
authors.forename: S
authors.initials: S
authors.lastname: Mizutani
mesh_terms: D000626:Aminopeptidases / Q000235:genetics*; D002822:Choriocarcinoma / Q000201:enzymology*; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D043384:Glutamyl Aminopeptidase; D006801:Humans; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000201:enzymology*
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D011980:Receptors, Progesterone; C076819:angiotensinase; D011374:Progesterone; D010450:Endopeptidases; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase