title: Trichuris suis: detection of antibacterial activity in excretory-secretory products from adults.
abstract: Antibacterial activity was detected in excretory-secretory products (ESP) of adult Trichuris suis cultured in vitro in serum-free media. Gram-negative bacteria (Campylobacter jejuni, Campylobacter coli, and Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus) were sensitive to ESP. Susceptibility was dependent on the concentration of ESP but not on the inoculum size. Preliminary assessment of the mode of action suggests a bacteriocidal mechanism. This antibacterial activity was heat stable and resistant to digestion with pronase E and trypsin. Based on ultrafiltration experiments, the activity is less than 10,000 MW. This excreted/secreted antibacterial activity from T. suis is likely a component of a humoral defense system for this helminth.
authors.affiliation: National Food Safety and Toxicology Center, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Abner
authors.forename: G
authors.initials: G
authors.lastname: Parthasarathy
authors.forename: D E
authors.initials: DE
authors.lastname: Hill
authors.forename: L S
authors.initials: LS
authors.lastname: Mansfield
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017000:Campylobacter coli / Q000187:drug effects* / Q000254:growth & development; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000254:growth & development; D002701:Chloramphenicol / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008826:Microbial Sensitivity Tests; D013552:Swine; D014258:Trichuris / Q000737:chemistry* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol