Characterization of recombinant Desulfovibrio gigas ferredoxin.
Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared.
Rodrigues
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P
Departamento de Química, Centro Quìmica Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825 Monte de Caparica, Portugal.
Graça
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Macedo
A L
AL
Moura
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Moura
J J
JJ
D003001:Cloning, Molecular; D003901:Desulfovibrio / Q000737:chemistry*; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D014466:Ultraviolet Rays
D005288:Ferredoxins; D011994:Recombinant Proteins; D013455:Sulfur; D007501:Iron; D010100:Oxygen