title: Activin disrupts somitogenesis in cultured chick embryos.
abstract: The anatomical and cell biological aspects of somite formation in the chick embryo have been rather well studied. Molecular regulation of somitogenesis in vertebrates is just beginning to be understood. We have studied the effects of human recombinant activin on somitogenesis in gastrulating chick embryos cultured in vitro with a view to assessing the possible role of activin-related molecules in this phenomenon. Activin disrupted somitogenesis in treated embryos, resulting in the formation of abnormal, split or ectopic somites. Light microscopic examination indicated that the ability of activin to interfere with somitogenesis might be partly due to initiation of somite formation at ectopic sites. We show that these cells are indeed somitogenic by their expression of one of the earliest somite-specific marker genes, Pax3. Scanning electron microscopic examination of control and treated embryos revealed direct effects of activin on cell-cell interactions. Cells from treated embryos exhibited disrupted intercellular adhesion leading to large intercellular spaces, altered cell shapes and modification of cell surface protrusions. The effects of activin on somitogenesis appear to be specific, since the neural structures, which are generally more susceptible to chemical insults during gastrulation, were relatively less affected. The results clearly point to a role of activin-related molecules in somitogenesis in the chick embryo.
authors.affiliation: Division of Animal Sciences, Agharkar Research Institute, G.G. Agarkar Road, Pune-411 004, India.
authors.forename: V
authors.initials: V
authors.lastname: Patwardhan
authors.forename: S
authors.initials: S
authors.lastname: Ghaskadbi
mesh_terms: D028341:Activins / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo / Q000166:cytology / Q000187:drug effects* / Q000196:embryology* / Q000648:ultrastructure; D046508:Culture Techniques; D016373:DNA, Antisense / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D017403:In Situ Hybridization; D008855:Microscopy, Electron, Scanning; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D019170:Somites / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D014157:Transcription Factors
chemical_list: D016373:DNA, Antisense; D004268:DNA-Binding Proteins; D000072041:PAX3 Transcription Factor; C489409:PAX3 protein, human; D051761:Paired Box Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D028341:Activins; C068686:Pax3 protein, mouse