Site-specific incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (Ni(2+):nitrilotriacetic Acid (n)-fluorochrome conjugates.
Kapanidis
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Howard Hughes Medical Institute, Waksman Institute and Department of Chemistry, Rutgers University Piscataway New Jersey 08854.
Ebright
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YW
Ebright
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D005456:Fluorescent Dyes / Q000737:chemistry*; D006639:Histidine / Q000031:analogs & derivatives; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D005456:Fluorescent Dyes; D011506:Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid