title: [Usefulness of local administration of methotrexate bound to activated carbon particles (MTX-CH)].
abstract: We developed a new dosage formulation, methotrexate bound to activated carbon particles (MTX-CH), and used it to reduce tumors via its long-acting effect at the administration sites. MTX-CH was injected locally into tumors on the back of BALB/c mice, 30 mg/mouse, as MTX and compared with mice treated with MTX aqueous solution, saline solution, activated carbon particles (CH-40) and non-treated mice. The MTX concentration at the administration sites was higher in the MTX-CH group than in the MTX aqueous solution group. A marked effect on the control of tumor growth by MTX-CH was noted after repeated administration (every 3 days, total 4 times) throughout the observation period. Although tumor size was not reduced, necrosis was microscopically observed around the site of MTX-CH administration. For the reasons mentioned above, MTX-CH is superior to MTX aqueous solution in terms of long-acting effect at the administration sites and the control of tumor growth.
authors.affiliation: Dept. of Digestive Surgery, Kyoto Prefectural University of Medicine.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Shimomura
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: A
authors.initials: A
authors.lastname: Toma
authors.forename: J
authors.initials: J
authors.lastname: Fujiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Sonoyama
authors.forename: C
authors.initials: C
authors.lastname: Sakakura
authors.forename: E
authors.initials: E
authors.lastname: Ootsuji
authors.forename: A
authors.initials: A
authors.lastname: Hagiwara
authors.forename: H
authors.initials: H
authors.lastname: Yamagishi
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002244:Carbon; D003692:Delayed-Action Preparations; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000964:Antimetabolites, Antineoplastic; D003692:Delayed-Action Preparations; D002244:Carbon; D008727:Methotrexate