Isolation and sequencing of infectious clones of feline foamy virus and a human/feline foamy virus Env chimera.
Full-length DNAs of the Coleman and S7801 strains (pSKY3.0, pSKY5.0) of infectious feline foamy viruses (FFVs) were cloned and sequenced. Parental viruses, designated SKY3.0 and SKY5.0, were secreted following transfection of Crandell feline kidney (CRFK) cells. Production of the rescued parental viruses was enhanced in the presence of trichostatin A. Amino acid sequence similarities between FFV and human foamy virus (HFV) are extremely low for the envelope protein and capsid antigen, as predicted from the two clones. However, a chimeric FFV clone was constructed with the HFV Env substituted for the FFV Env. The chimeric virus (HFFV, SKY4.0) was able to infect and replicate in CRFK cells as well as in peripheral blood mononuclear cells of cats in vivo. Consequently, the chimeric HFFV may be useful for the creation of FV vectors for gene transfer strategies.
Hatama
Shinichi
S
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan3.|Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
Otake
Kaori
K
Department of Immunology, National Institute of Infectious Disease, Tokyo 113-8657, Japan2.
Omoto
Shinya
S
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
Murase
Yasunori
Y
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
Ikemoto
Atushi
A
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
Mochizuki
Masami
M
Central Laboratory, Kyoritu Shouji Co. Ltd, Ibaraki, Japan4.
Takahashi
Eiji
E
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan3.
Okuyama
Harumi
H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
Fujii
Yoichi
Y
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
D000818:Animals; D002213:Capsid / Q000235:genetics; D002371:Cat Diseases / Q000097:blood / Q000821:virology*; D002415:Cats; D002460:Cell Line / Q000821:virology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification*; D005822:Genetic Vectors; D015726:Giant Cells / Q000821:virology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009000:Monocytes / Q000821:virology; D011995:Recombination, Genetic; D012192:Retroviridae Infections / Q000662:veterinary*; D016092:Spumavirus / Q000235:genetics* / Q000472:pathogenicity; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D006877:Hydroxamic Acids; D014759:Viral Envelope Proteins; C012589:trichostatin A