Compaction, stainability and methylation of the late replicating X chromosome in mouse female fibroblasts.
In metaphases from female mouse fibroblasts, successive stainings by Giemsa and DAPI and immunolabeling of 5-methylcytosine were performed with or without bromodeoxyuridine pretreatment. It was shown that, compared to all other chromosomes, the late replicating X is the least methylated, the most compacted, and the most intensely stained by DAPI and Giemsa.
Bernardino-Sgherri
J
J
Laboratoire de Radiosensibilité des cellules germinales, DRR/DSV/CEA, Fontenay-aux-roses, France. bernardi@dsvidf.cea.fr
Dutrillaux
B
B
D044503:5-Methylcytosine; D000818:Animals; D001399:Azure Stains / Q000378:metabolism; D001973:Bromodeoxyuridine; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D019175:DNA Methylation; D004261:DNA Replication; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D008677:Metaphase; D008968:Molecular Conformation; D013194:Staining and Labeling; D014960:X Chromosome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D001399:Azure Stains; D002843:Chromatin; D007211:Indoles; C007293:DAPI; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D001973:Bromodeoxyuridine