Enzyme-modulated cleavage of dsDNA for studying interfacial biomolecular interactions.
This work describes the chemistry and methodology for constructing multilayers of bis-biotinylated dsDNA on metal substrates after enzyme cleavage and demonstrates its use for amplified microgravimetric and impedimetric analyses of anticancer drug, cisplatin. Specific chemical modification of dsDNA prior to immobilization was achieved via a bisulfite-catalyzed transamination of cytosine after endonuclease cleavage of plasmid DNA. The specificity of the reaction of cytosine residues at ss- versus dsDNA loci after endonuclease cleavage was characterized using circular dichroism, mass spectrometry, and absorption spectrophotometry. The biotinylated dsDNA consisting of 2961 base pairs was then used as a ligand at avidin-modified gold electrodes. Ac impedance spectroscopy and quartz crystal microbalance measurements clearly showed that the response to cisplatin increased linearly with target concentrations. The impedance spectroscopy resulted in a detection limit of 1 nM and a surface density of 4.8 x 10(13) molecules/0.1 cm(2). The immobilization of dsDNA on surfaces is a significant improvement over existing approaches in that it enables the attachment of long pieces of unmodified double-stranded DNA via a simple biotinylation step. The immobilization technique provides a generic approach for dsDNA-based sensor development and for monitoring DNA-analyte interactions.
Yan
F
F
Department of Chemistry, State University of New York at Binghamton, P.O. Box 6016, Binghamton, NY 13902, USA.
Sadik
O A
OA
D001710:Biotin / Q000737:chemistry; D002942:Circular Dichroism; D003596:Cytosine / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D017097:Electric Impedance; D004566:Electrodes; D004720:Endonucleases / Q000737:chemistry* / Q000378:metabolism; D006046:Gold; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013696:Temperature
D004277:DNA, Single-Stranded; D001710:Biotin; D006046:Gold; D003596:Cytosine; D004247:DNA; D004720:Endonucleases