title: Tumour-related enzyme alterations in the clear cell type of human renal cell carcinoma identified by two-dimensional gel electrophoresis.
abstract: To identify tumour-related enzyme alterations we have used 2D-gels to analyse the proteome from dissected malignant and benign kidney areas from patients with clear-cell-type renal carcinoma. The expression of 12 proteins was diminished in tumour. Four proteins were characterized by mass spectrometry and were identified as enoyl-CoA hydratase, alpha-glycerol-3-phosphate dehydrogenase, aldehyde dehydrogenase 1 and aminoacylase-I.
authors.affiliation: Department of Medical Biochemistry and Molecularbiology, University of Greifswald, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Balabanov
authors.forename: U
authors.initials: U
authors.lastname: Zimmermann
authors.forename: C
authors.initials: C
authors.lastname: Protzel
authors.forename: C
authors.initials: C
authors.lastname: Scharf
authors.forename: K J
authors.initials: KJ
authors.lastname: Klebingat
authors.forename: R
authors.initials: R
authors.lastname: Walther
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism*; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000473:pathology; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D004746:Enoyl-CoA Hydratase / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000473:pathology; D010449:Peptide Mapping
chemical_list: D017931:DNA Primers; D005993:Glycerolphosphate Dehydrogenase; D000444:Aldehyde Dehydrogenase; D000581:Amidohydrolases; C019672:aminoacylase I; D004746:Enoyl-CoA Hydratase