Expression and preliminary X-ray diffraction studies of cytosolic Cu,Zn superoxide dismutase from Schistosoma mansoni.
Active cytosolic (CT) Cu,Zn superoxide dismutase from Schistosoma mansoni (SmCTSOD) was recovered after thrombin cleavage of a glutathione-S-transferase linked fusion protein (GST-SmCTSOD) expressed in the presence of the active-site metals. Crystals have been obtained in two space groups, P2(1)2(1)2(1) and P2(1). The former have unit-cell parameters a = 74.64, b = 78.24, c = 95.18 A and typically diffract to 2.2 A. The monoclinic crystals have unit-cell parameters a = 39.27, b = 95.08, c = 78.41 A, beta = 103.55 degrees and diffract to at least 1.55 A. The calculated solvent content of the crystals is compatible with two dimers of SmCTSOD in the asymmetric unit in both cases. Molecular-replacement solutions have been obtained for both crystal forms and show that slight distortions in the crystal packing relate one form to the other.
Cardoso
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RM
Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador Sancarlense 400, São Carlos, SP, 13566-590, Brazil.
da Silva
C H
CH
de Araújo
A P
AP
Tanaka
T
T
Tanaka
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M
Garratt
R C
RC
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology; D008958:Models, Molecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012550:Schistosoma mansoni / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000737:chemistry*
D011994:Recombinant Proteins; D013482:Superoxide Dismutase