Human T and B cell epitope mapping of Taenia solium paramyosin.
Taenia solium paramyosin is an immunodominant antigen in human and porcine cysticercosis that has shown promise as a vaccine candidate against schistosomiasis and some filariasis. There are few studies to identify the immunologically relevant regions of paramyosin. In this work, we characterize the humoral and cellular response of neurocysticercotic patients against T. solium paramyosin. Western blots using different recombinant fragments of T. solium paramyosin, showed that the sera from neurocysticercotic patients were strongly reactive against the carboxyl end region, with poor recognition of the central and amino regions. In contrast, the cellular immune response of patients did not show preferential recognition of any region of paramyosin.
Vázquez-Talavera
J
J
Department of Immunology, Instituto de Investigaciones Biomédicas, UNAM, México.
Solís
C F
CF
Medina-Escutia
E
E
López
Z M
ZM
Proaño
J
J
Correa
D
D
Laclette
J P
JP
D000818:Animals; D015153:Blotting, Western; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D020019:Neurocysticercosis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013621:Taenia / Q000276:immunology*
D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D015801:Helminth Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins