title: Diminished corneal angiogenesis in gelatinase A-deficient mice.
abstract: The goal of the present study was to define the role of gelatinase A in angiogenesis. We performed corneal micropocket assays in gelatinase A-deficient mice and their age-matched wild-type littermates. The corneal neovascular area in gelatinase A-deficient mice (0.15+/-0.14 mm(2)) was significantly less than that of wild-type littermates (0.53+/-0.35 mm(2); P<0.01). Similarly, aortic ring assays showed significant reduction of endothelial outgrowth in gelatinase A-deficient mice (0.26+/-0.14 mm(2)) as compared to wild-type littermates (0.44+/-0.06 mm(2); P<0.05). These results suggest that gelatinase A may play an important role in the regulation of corneal angiogenesis.
authors.affiliation: The Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Kure
authors.forename: J H
authors.initials: JH
authors.lastname: Chang
authors.forename: E E
authors.initials: EE
authors.lastname: Gabison
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Itohara
authors.forename: D T
authors.initials: DT
authors.lastname: Azar
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003315:Cornea / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D016510:Corneal Neovascularization / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D017353:Gene Deletion; D005838:Genotype; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D018919:Neovascularization, Physiologic / Q000187:drug effects
chemical_list: D016222:Fibroblast Growth Factor 2; D020778:Matrix Metalloproteinase 2