Further evaluation of an in vivo micronucleus test on rat and mouse skin: results with five skin carcinogens.
In a previous paper, we presented a practical in vivo micronucleus (MN) test that used rat skin as the target organ. To evaluate the test, as well as to determine the reproducibility and applicability of the method to mice, we used it to test the effect of five skin carcinogens (N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline 1-oxide (4NQO), 7,12-dimethylbenz[a]anthracene (DMBA), and benzo[a]pyrene (B[a]P)) on rat and mouse skin. All five compounds significantly and dose-dependently increased the MN frequencies in the basal cells of the chemical-treated skin. These results indicated the reproducibility of the test results and also the applicability of the test to mice as well as rats.
Nishikawa
Takashi
T
Human Safety Evaluation Center, Lion, 100 Tajima, Odawara-shi, Kanagawa 256-0811, Japan. ntaka@lion.co.jp
Haresaku
Mitsuru
M
Fukushima
Akira
A
Nakamura
Tsuneaki
T
Adachi
Kuniaki
K
Masuda
Mitsuteru
M
Hayashi
Makoto
M
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects*
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D001564:Benzo(a)pyrene; C013525:ENNG; D015112:4-Nitroquinoline-1-oxide; D015127:9,10-Dimethyl-1,2-benzanthracene