WBC content of platelet concentrates prepared by the buffy coat method using different processing procedures and storage solutions.
BACKGROUND
The number of WBCs in platelet concentrates (PCs) prepared by the buffy coat (BC) method with different storage solutions can result in low (5 x 10(6)/unit) WBC levels by the use of careful centrifugation techniques without filtration. At present, most blood banks use filtration steps to meet these requirements. The difference in processing methods and suspension solutions prompted the investigation of the influence of the various procedures on the WBC and platelet content of PCs.


METHODS
PCs from 5 BCs were harvested without or with inline filtration (AutoStop BC, Pall Corp.) in either plasma (PCs-plasma) or platelet additive solution (PCs-PAS-2). After preparation, samples were taken for counting WBCs and platelets and for analyzing WBC subsets by flow cytometry using specific MoAbs. The WBCs were concentrated before analysis of the WBC subsets. Results less than 2.5 cells per microL were considered below the limit of accuracy of the subset analysis.


RESULTS
All filtered PCs met the AABB standard of 5 x 10(6) per unit and the European guidelines of 1 x 10(6) per unit. None of the nonfiltered PCs met the European guidelines, but all met the AABB guidelines. All filtered units gave residual WBC counts below the detection limit for subset analysis. Filtered PCs-plasma gave significantly higher platelet counts than filtered PCs-PAS-2 or nonfiltered PCs (p<0.01, ANOVA).


CONCLUSIONS
Careful centrifugation of pooled BCs, with plasma or PAS-2, can result in PCs with low WBC contamination levels. However, filtered PCs are superior, because of better WBC removal and higher platelet counts.
de Wildt-Eggen
J
J
Sanquin Blood Bank, Ijssellanden Division, Zwolle, the Netherlands. jdewildt@bloedbankijssellanden.nl
Schrijver
J G
JG
Bins
M
M
D000085:Acetates; D001793:Blood Preservation; D002498:Centrifugation / Q000592:standards*; D002951:Citrates; D006440:Hemofiltration / Q000592:standards*; D006801:Humans; D007958:Leukocyte Count; D008722:Methods; D010949:Plasma; D010976:Platelet Count; D011310:Preservatives, Pharmaceutical; D012965:Sodium Chloride
D000085:Acetates; D002951:Citrates; C436265:PAS-2 salt solution; D011310:Preservatives, Pharmaceutical; D012965:Sodium Chloride