Multiple reactive immunization towards the hydrolysis of organophosphorus nerve agents: hapten design and synthesis.
We designed and synthesized a series of haptens to elicit catalytic antibodies with phosphatase activity against nerve agents. The design is based on the novel concept of multiple reactive immunization which aims to afford two or more catalytic residues within the antibody's binding cleft. The haptens showed the desired reactivity in vitro and were submitted for immunization.
Huang
H
H
The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Han
W G
WG
Noodleman
L
L
Grynszpan
F
F
D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000378:metabolism; D001671:Biochemistry / Q000379:methods; D002619:Chemical Warfare Agents / Q000737:chemistry / Q000378:metabolism*; D015195:Drug Design; D006241:Haptens / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D006868:Hydrolysis; D007114:Immunization / Q000379:methods; D008958:Models, Molecular; D018696:Neuroprotective Agents / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism*
D017151:Antibodies, Catalytic; D002619:Chemical Warfare Agents; D006241:Haptens; D018696:Neuroprotective Agents; D009943:Organophosphorus Compounds; C448864:hapten 44; C448865:hapten 7