Leukocyte receptor complex-encoded immunomodulatory receptors show differing specificity for alternative HLA-B27 structures.
We studied recognition of the disease-associated HLA-B27 allele by immunomodulatory receptors encoded within the leukocyte receptor complex. HLA class I are ligands for members of the killer Ig receptor (KIR) and Ig-like transcript (ILT)/LIR/LILR families (the new LILR nomenclature is described at www. gene.ucl.ac.uk/nomenclature/genefamily/lilr.html). Members of these families bound HLA-B27 in both classical and beta(2) microglobulin-independent forms. Classical complexes bound ILT2, ILT4, and LIR6 transfectants but not ILT1, ILT3, or ILT5. A free H chain form of HLA-B27 bound ILT4 and LIR6. Both forms of HLA-B27 bound KIR3DL1 transfectants. HLA-B27 free H chain bound CD14(+) cells in PBL from healthy controls, consistent with ILT4 expression on monocytes. Alternative recognition of different forms of HLA-B27 by KIR or ILT could influence their immunomodulatory function and may imply a role in inflammatory disease.
Allen
R L
RL
Department of Pathology, University of Cambridge, Cambridge, United Kingdom. rla25@mole.bio.cam.ac.uk
Raine
T
T
Haude
A
A
Trowsdale
J
J
Wilson
M J
MJ
D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D015796:HLA-B27 Antigen / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008214:Lymphocytes / Q000276:immunology; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; D014162:Transfection
D015703:Antigens, CD; D015796:HLA-B27 Antigen; C515083:KIR3DL1 protein, human; C105298:LILRA2 protein, human; C105299:LILRB1 protein, human; C491341:LILRB2 protein, human; D000075362:Leukocyte Immunoglobulin-like Receptor B1; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054341:Receptors, KIR3DL1; C413086:leukocyte-immunoglobulin--like receptor 6