Serial pentamidine levels in bronchial epithelial lining fluid after aerosol administration.
BACKGROUND
There is no information on serial pharmacokinetic assessment in the lungs after administration of aerosolized pentamidine.


OBJECTIVE
The present study was performed to evaluate the elimination of aerosolized pentamidine from bronchial airways following inhalation.


METHODS
We used 4 sheep with tracheotomies in the present study. Pentamidine (300 mg) was administered by inhalation to each animal. Serial bronchial washing to obtain epithelial lining fluid (ELF) was performed 1, 7, 10, 14, 21 and 28 days after administration of aerosolized pentamidine in each animal. The pentamidine concentration in the supernatant of ELF was measured by high-performance liquid chromatography.


RESULTS
The maximal pentamidine level on the first day (12 h after inhalation) was 616.5 +/- 238.2 ng/ml (mean +/- SE) in ELF. The pentamidine levels rapidly decreased within 2 weeks (8.9 +/- 1.2 ng/ml at 14 days), followed by slow elimination (8.9 +/- 0.8 ng/ml at 28 days). Thus, inhaled pentamidine showed a rapid clearance from the bronchial wall within the first 2 weeks.


CONCLUSIONS
These findings may be useful in designing and interpreting future studies of aerosolized pentamidine in patients who are receiving inhaled pentamidine, especially for those with failure of prophylaxis for Pneumocystis carinii pneumonia.
Yamamoto
H
H
First Department of Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Koizumi
T
T
Miyahara
T
T
Kaneki
T
T
Kubo
K
K
D000280:Administration, Inhalation; D000818:Animals; D000935:Antifungal Agents / Q000097:blood / Q000493:pharmacokinetics*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D023421:Models, Animal; D010419:Pentamidine / Q000097:blood / Q000493:pharmacokinetics*; D012756:Sheep; D013997:Time Factors
D000935:Antifungal Agents; D010419:Pentamidine