High-performance genetic analysis using microfabricated capillary array electrophoresis microplates.
This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (microCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of sequencing and genotyping data from radially formatted microCAE devices. The concomitant development of a convenient energy-transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High-performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10-100 times that of current technology.
Medintz
I L
IL
Department of Chemistry, University of California, Berkeley 94720, USA.
Paegel
B M
BM
Blazej
R G
RG
Emrich
C A
CA
Berti
L
L
Scherer
J R
JR
Mathies
R A
RA
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000032:analysis; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods; D005821:Genetic Techniques / Q000295:instrumentation; D015894:Genome, Human; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics; D006432:Hemochromatosis / Q000235:genetics; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007834:Lasers; D008565:Membrane Proteins; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes; C103673:HFE protein, human; D006680:HLA Antigens; D000071020:Hemochromatosis Protein; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins