A study of moss (Marchantia polymorpha) thylakoid membrane lipids in monolayers.
Monolayers of seven fractions of natural lipids (phosphatidyl inositol, sulfoquinovosyl diacylglycerol, phosphatidylcholine, digalactosyl diacylglycerol, phosphatidyl glycerol, phosphatidylethanolamine, monogalactosyl diacylglycerol), isolated from the photoautotrophic cell culture of the moss Marchantia polymorpha grown under normal and light-stress conditions, have been prepared for the first time. We have shown that the high-intensity light affects the area occupied by the lipid molecule. In the case of digalactosyl diacylglycerol and phosphatidyl glycerol fractions, after the light stress the area significantly increased from 0.50 to 0.80 nm2 and from 0.47 to 0.63 nm2, respectively, and in the case of the sulfoquinovosyl diacylglycerol fraction, the area decreased from 0.40 to 0.32 nm2. These results are in agreement with our previous data on the redistribution of the double bonds in the aliphatic chains of these lipids and can be used to characterize the state of the lipid bilayer of the thylakoid membranes.
Volchenkova
T A
TA
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Kalabina
N A
NA
Schaefer
C
C
Zubov
V P
VP
Zaitsev SYu
D019068:Bryopsida / Q000737:chemistry / Q000528:radiation effects; D002478:Cells, Cultured / Q000737:chemistry / Q000528:radiation effects; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000737:chemistry / Q000302:isolation & purification; D038983:Galactolipids; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification; D008027:Light; D008563:Membrane Lipids / Q000737:chemistry* / Q000302:isolation & purification; D010713:Phosphatidylcholines / Q000737:chemistry / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000302:isolation & purification; D010715:Phosphatidylglycerols / Q000737:chemistry / Q000302:isolation & purification; D010716:Phosphatidylinositols / Q000737:chemistry / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry* / Q000528:radiation effects; D020524:Thylakoids / Q000737:chemistry* / Q000528:radiation effects
D004075:Diglycerides; D038983:Galactolipids; D006017:Glycolipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; C013694:sulfoquinovosyl diglyceride