A dominant antigenic region of the hantaan virus nucleocapsid protein is located within a amino-terminal short stretch of hydrophilic residues.
The nucleocapsid (N) protein of the Hantaan virus (HTNV) is a major viral antigen that induces a strong antibody response during the acute phase of infection. By immunoblot analyses of the recombinant N proteins using human sera of the hemorrhagic fever with renal syndrome (HFRS), we have confirmed previous finding by other investigators of the presence of a highly antigenic region near the amino terminus of the HTNV N protein. We have further located the antigenic region within a short stretch of hydrophilic sequences between the 26 and the 46th amino acid residues. The recombinant glutathione S-transferase fusion proteins containing this region was expressed as a soluble form in a large quantity in Escherichia coli, and purified by a single-step affinity chromatography. The recombinant antigen also showed a similar, but a weaker reactivity with human antisera to Seoul virus (SEOV), the virus most closely related to HTNV.
Kang
J I
JI
Graduate School of Life Science and Biotechnology, Korea University, Seoul.
Lee
Y S
YS
Ahn
K
K
Song
J W
JW
Song
K J
KJ
Baek
L J
LJ
Lee
P W
PW
Ahn
B Y
BY
D000956:Antigens, Viral / Q000737:chemistry*; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry* / Q000276:immunology; D036022:Capsid Proteins; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D014758:Viral Core Proteins / Q000737:chemistry* / Q000276:immunology
D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus