title: A capillary electrophoretic system with an improved gas-phase chemiluminescence nitrogen detector.
abstract: A new, rugged nebulizer interface was designed to couple a capillary electrophoretic system to a gas-phase nitrogen chemiluminescence detector. By hydraulically decoupling the separation capillary from the nebulizer of the gas-phase nitrogen chemiluminescence detector, the interface-related loss of separation efficiency could be limited to a mere 10%. The ozone reaction chamber of the gas-phase nitrogen chemiluminescence detector has been redesigned leading to a threefold improvement in detection sensitivity over the old design. In combination with a field-amplified sample stacking procedure, the new system was used to detect 0.25 ppm concentrations of UV chromophore-free aminoglycosides.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
authors.forename: A D
authors.initials: AD
authors.lastname: Sokolowski
authors.forename: G
authors.initials: G
authors.lastname: Vigh
mesh_terms: D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D008163:Luminescent Measurements; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000617:Aminoglycosides