title: Possible origin of extrachromosomal DNA from human lymphocytes following in vitro treatment by phytohemagglutinin.
abstract: Lymphocytes respond to chemical or pathological challenges by synthesizing episomes or extrachromosomal DNA (eDNA). Phytohemagglutinin (PHA) is a mitogen for T lymphocytes and can stimulate the increased production of episomes as well as the release of eDNA, interleukin-2, and other factors. However, the origin of this eDNA is largely unknown. To confirm the observation on the localization of an antibody directed against a 23-kb segment of bull acrosomal DNA in cultured human lymphocytes, we performed analyses by fluorescence in situ hybridization (FISH) using the same DNA as a molecular probe.
authors.affiliation: Centro per lo Studio delle Cellule Germinale, Consiglio Nazionale delle Ricerche, Siena, Italy. chen@unisi.it
authors.forename: J S
authors.initials: JS
authors.lastname: Chen
authors.forename: M G
authors.initials: MG
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology*
chemical_list: D010835:Phytohemagglutinins; D004247:DNA