title: Dominant lesional T cell receptor rearrangements persist in relapsing psoriasis but are absent from nonlesional skin: evidence for a stable antigen-specific pathogenic T cell response in psoriasis vulgaris.
abstract: In a previous study we reported that clonally expanded T cell receptor beta-chain rearrangements characterized the T cell receptor usage in skin lesions of psoriasis vulgaris and indicated antigen-specific T cell selection. To assess the relevance of clonal T cell expansion for disease progression, we now determined if select clonal T cell receptor rearrangements persisted over time and were present in nonlesional skin. Sequential biopsies were taken from psoriatic skin lesions of two patients. V-D-J junctional regions of T cell receptor beta-chain variable region gene families 2, 3, 6, 13S1, and BV17 were cloned and sequenced, as these particular BV gene families are preferentially selected in psoriatic skin lesions. The lesional T cell receptor rearrangements were compared with the T cell receptor usage in nonlesional skin and in blood. Several T cell receptor beta-chain rearrangements with high transcript frequency in the first lesional biopsy were again found in sequential lesional biopsies taken as much as 3 y later from psoriasis relapses. Only T cell receptor beta-chain rearrangements with low transcript abundance showed variability in that several clones appeared for the first time or disappeared. Although nonlesional skin also exhibited a restricted T cell receptor usage with clonal T cell receptor rearrangements, the T cell receptor usage in lesional and nonlesional skin differed nearly completely. The select lesional recurrence of identical T cell receptor rearrangements reveals that inflammation in psoriasis involves the same clonally expanded T cell populations and the same antigens over prolonged periods of time. It hereby suggests that specifically recruited and locally expanded T cell clones are permanently involved in psoriatic inflammation and may play a crucial part in disease perpetuation.
authors.affiliation: Department of Dermatology, Ludwig Maximilians-University, Munich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Vollmer
authors.forename: A
authors.initials: A
authors.lastname: Menssen
authors.forename: J C
authors.initials: JC
authors.lastname: Prinz
mesh_terms: D000939:Epitopes; D015321:Gene Rearrangement; D005799:Genes, Dominant; D006801:Humans; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D012008:Recurrence; D012867:Skin / Q000473:pathology* / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D000939:Epitopes; D016693:Receptors, Antigen, T-Cell, alpha-beta