Over-expression of the functional interleukin-11 alpha receptor in the development of B-cell chronic lymphocytic leukemia.
Several cytokines have been found to play a role in the pathogenesis of B-CLL. In the present study using reverse-transcriptase polymerase chain reaction (RT-PCR), FACS analysis and immunofluorescence we have shown the constitutive expression of IL-11 and IL-11R alpha in B-chronic lymphocytic leukemia (B-CLL). The expression level of IL-11R alpha in B-CLL cells is much higher than in PBL of normal donors. Recombinant human IL-11 (rhIL-11) activates B-CLL cells, leading to morphologic alterations of the cells and increase in cell number and size. Short-term cultivation in the presence of rhIL-11 did not lead to quantitative changes in the ratio of the living vs apoptotic and dead cells. However, in contrast to rhIL-6, pretreatment with rhIL-11, did not cause B-CLL cells to be resistant to the action of dexamethasone. These data suggest an essential role for the IL-11/IL11 R alpha system in the pathogenesis of the malignant B-CLL cells.
Tsimanis
A
A
Department of Hematology, Kaplan Medical Center, POB 1, Rehovot, 76100, Israel. Yehudats@netvision.net.il
Shvidel
L
L
Klepfish
A
A
Shtalrid
M
M
Kalinkovich
A
A
Berrebi
A
A
D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D006801:Humans; D017370:Interleukin-11 / Q000235:genetics / Q000494:pharmacology; D053703:Interleukin-11 Receptor alpha Subunit; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood* / Q000209:etiology; D008213:Lymphocyte Activation / Q000187:drug effects; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D018123:Receptors, Interleukin / Q000235:genetics*; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
C508428:IL11RA protein, human; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins