title: Crystallization and preliminary X-ray analysis of neural haemoglobin from the nemertean worm Cerebratulus lacteus.
abstract: The nemertean worm Cerebratulus lacteus neural tissue haemoglobin (109 amino acids, the shortest known haemoglobin) has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant native data set has been collected at the Cu K(alpha) wavelength to 2.05 A resolution. The crystals belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 42.5, b = 43.1, c = 60.2 A and one molecule per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe atom, thus paving the way for MAD/SAD structure determination.
authors.affiliation: Department of Physics - INFM, Advanced Biotechnology Centre, University of Genova, Largo Rosanna Benzi 10, I-16146 Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Pesce
authors.forename: M
authors.initials: M
authors.lastname: Nardini
authors.forename: S
authors.initials: S
authors.lastname: Dewilde
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: A F
authors.initials: AF
authors.lastname: Riggs
authors.forename: K
authors.initials: K
authors.lastname: Yamauchi
authors.forename: E
authors.initials: E
authors.lastname: Geuens
authors.forename: L
authors.initials: L
authors.lastname: Moens
authors.forename: M
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005057:Eukaryotic Cells; D006454:Hemoglobins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation
chemical_list: D006454:Hemoglobins