Expression of FasL gene in T cells of renal allograft recipients.
FasL molecule expressed on activated T cells induces apoptosis in Fas-expressing cells. It is possible that apoptosis induced by FasL is involved in the process of allograft destruction brought about by infiltrating T cells. The aim of our study was to evaluate expression of FasL gene in peripheral blood T cells of renal allograft recipients (RAR). We have studied 25 patients: 16 with uneventful stable course (RAR-S) and nine during biopsy proven chronic rejection (RAR-CH). The relative expression of FasL mRNA compared with that of beta-actin was established by semi-quantitative RT-PCR. We have found that FasL gene expression was significantly increased in T cells of RAR-CH compared to RAR-S (P<0.01). Our results suggest that T cell expression of FasL gene is increased during chronic rejection. Therefore, this phenomenon may pay a role in allograft injury associated with that process. Further studies are needed to unravel possible clinical consequences of observed differences in T cell expression of FasL.
Kasprzycka
Monika
M
Department of Clinical Immunology, Transplantation Institute, The Medical University of Warsaw, Nowogrodzka 59 str., 02-006, Warsaw, Poland. monika-kasprzycka@wp.pl
Kłodos
Konrad
K
Nowaczyk
Maria
M
Wyzgał
Janusz
J
Podobińska
Iwona
I
Durlik
Magdalena
M
Górski
Andrzej
A
D000328:Adult; D017209:Apoptosis; D001379:Azathioprine / Q000627:therapeutic use; D016572:Cyclosporine / Q000627:therapeutic use; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000188:drug therapy / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008875:Middle Aged; D011241:Prednisone / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D016572:Cyclosporine; D001379:Azathioprine; D011241:Prednisone