Molecular characterization of first human Bartonella strain isolated in Italy.
The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain.
Ciervo
A
A
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy.
Petrucca
A
A
Ciarrocchi
S
S
Pinto
A
A
Bonazzi
L
L
Fabio
A
A
Farnetti
E
E
Chomel
B B
BB
Ciceroni
L
L
D000328:Adult; D016917:Angiomatosis, Bacillary / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000379:methods; D018413:Bartonella quintana / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000032:analysis; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007558:Italy; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S