Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant.
Tumor growth is the result of combined cell proliferation overwhelming cell death and neoangiogenesis. This report shows CAM angiogenesis promoted by TA3 tumor supernatant with or without low dosis of betamethasone (Minimal antiangiogenic concentration: beta-MAAC). Methylcellulose discs instilled with 10 microliters of beta-MAAC (0.08 microgram/ml), 10 microliters of tumor supernatant (TA3ts), 5 microliters beta-MAAC + 5 microliters TA3ts, and 10 microliters of PBS as control were implanted in host chick eggs. On day 12, the grafts were removed, photographed and fixed. Sections were stained in parallel, one and three with hematoxylin-eosin, and section two by the Tunel method. The number of vessels was evaluated in a microscopic field of the CAM (2250 micron 2). The results show that beta-MAAC produced a significant inhibition of neovascularization in comparison to that observed in controls (P < 0.0025; Student t-Test). Discs instilled with TA3ts produced an intense stimulation of angiogenesis in contrast, when discs were instilled with 5 microliters of beta-MAAC + 5 microliters of TA3ts the angiogenesis was significantly inhibited (P < 0.001). The results show that effective antiangiogenic doses of betamethasone are in the range of 10(-7) M, (probably a genomic mediated action) and that this effect of low concentration may have clinical applications.
Lemus
D
D
Programa de Morfología, Instituto de Ciencias Biomédicas (ICBM) Facultad de Medicina, Universidad de Chile, Independencia 1027, Correo 7, Casilla 70079 Santiago 7. dlemus@machi.med.uchile.cl
Dabancens
A
A
Illanes
J
J
Fuenzalida
M
M
Guerrero
A
A
López
C
C
D000482:Allantois / Q000098:blood supply / Q000187:drug effects*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D001623:Betamethasone / Q000494:pharmacology* / Q000627:therapeutic use; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects; D006133:Growth Substances / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000473:pathology / Q000517:prevention & control*; D014407:Tumor Cells, Cultured
D020533:Angiogenesis Inhibitors; D006133:Growth Substances; D009363:Neoplasm Proteins; D001623:Betamethasone