Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay.
A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.
Wannet
W J
WJ
National Institute of Public Health and the Environment, Bilthoven, The Netherlands. wim.wannet@rivm.nl
Reessink
M
M
Brunings
H A
HA
Maas
H M
HM
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D020459:Genes, rRNA; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D013997:Time Factors; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
C069712:Ail protein, Yersinia enterocolitica; D001425:Bacterial Outer Membrane Proteins; D012336:RNA, Ribosomal, 16S