The utilization of sodium taurocholate in excystation of Cryptosporidium parvum and infection of tissue culture.
The present work deals with optimization of excystation of Cryptosporidium parvum oocysts and the infection process of tissue culture cells by the parasite. It was shown that presence of the bile salt sodium taurocholate in the incubation medium expedited excystation of the tested GCH1 isolate and enhanced it, as compared with bleaching of the oocysts. This bile salt had no effect on the viability of tissue culture cell lines MDBK and HCT-8 at the tested concentration of 0.375% for up to 2 hr of coincubation. Infection studies conducted on tissue culture cells showed higher infection rates in the presence of sodium taurocholate than with bleached oocysts in the absence of this bile salt. It may be concluded that, at least as regards the GCH1 strain of C. parvum, the whole infection process can be performed in the presence of sodium taurocholate, and does not require separation and cleaning of the excysted sporozoites before their application to tissue culture cells.
Gold
D
D
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
Stein
B
B
Tzipori
S
S
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004396:Coloring Agents / Q000737:chemistry; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D003902:Detergents / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000469:parasitology; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013656:Taurocholic Acid / Q000494:pharmacology*; D014343:Trypan Blue / Q000737:chemistry; D014407:Tumor Cells, Cultured
D004396:Coloring Agents; D003902:Detergents; D013656:Taurocholic Acid; D012973:Sodium Hypochlorite; D014343:Trypan Blue