[The inhibiting effect of antisense thrombin receptor gene on the proliferation of pig vascular smooth muscle cell].
OBJECTIVE
To search for an effective approach to prevent the formation of restenosis after angioplasty.


METHODS
A recombinant eukaryotic expression plasmid vector containing partial antisense thrombin receptor (ATR) gene named pcDNA3/ATR was constructed using recombinant DNA technique. Mini-pig aorta injury model was established by over-sized balloon catheter combined with high cholesterol diet feeding and its aortic smooth muscle cells(ASMC) were cultured. The effect of ATR gene expression in mini pig ASMC proliferation and growth factor gene expression were studied by 3H-TdR incorporation and Northern blot.


RESULTS
The DNA synthesis in pig ASMC could be inhibited by ATR gene expression (The DNA synthesis in normal ASMC was lowered by 41.8%, and that in ASMC from injured artery was lowered by (50.3%). The mRNA and protein synthesis of TR could be down regulated by ATR gene expression. The mRNA expression of PDGF-A chain and bFGF stimulated by fetal calf serum (FCS) with thrombin were both downregulated in pig ASMC with expressed ATR gene.


CONCLUSIONS
ATR gene expression can inhibit the proliferation of pig ASMC, and this is induced by its inhibiting effect on TR and finaly the signal transduction in ASMC.
Zhang
Q
Q
Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037.
Jiang
Y
Y
Liu
D
D
D000818:Animals; D001013:Aorta, Thoracic / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D016373:DNA, Antisense / Q000096:biosynthesis*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D018179:Receptors, Thrombin / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013556:Swine, Miniature; D014162:Transfection
D016373:DNA, Antisense; D018179:Receptors, Thrombin; D011994:Recombinant Proteins