High-sensitivity analysis of cyanide by capillary electrophoresis with fluorescence detection.
A capillary electrophoretic method for a high-sensitivity analysis of cyanide has been developed. Cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorescence detection. The detection limit was 0.1 ng/mL, and the calibration curve was linear over the range 0.1-200 ng/mL. The precision of the migration time of within-run assays (n = 6) of 1 ng/mL cyanide standard solution was 0.14%. The precision of the peak area for the same runs was 1.0%. This method was applicable to blood analysis. Detection of the cyanide derivative by UV was also examined.
Chinaka
S
S
Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 2-1-1 Hirosaka, Kanazawa 920-8553, Japan.
Tanaka
S
S
Takayama
N
N
Tsuji
N
N
Takou
S
S
Ueda
K
K
D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D003486:Cyanides / Q000032:analysis* / Q000097:blood; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007202:Indicators and Reagents; D009281:Naphthalenes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D003486:Cyanides; D007202:Indicators and Reagents; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde