title: Oral immunisation of chickens using cholera toxin B subunit and Softigen as adjuvants results in high antibody titre in the egg yolk.
abstract: Oral immunisation by gavage of laying hens with human immunoglobulin G (IgG) combined with a number of potential adjuvants was performed. The resulting immunospecific egg yolk (IgY) antibodies were quantified by ELISA. The following adjuvants were tested: A Poly(lactide-co-glycolide) (PLG) microspheres, Cholera toxin B-subunit (CTB), CTB conjugated with glutaraldehyde, Dimethyl dioctadecyl ammonium bromide (DDA), and Softigen (pegylated C8/C10 mono/di glyceride). Hens in a positive control group were immunised with human IgG in saline emulsified with an equal volume of Freund's Incomplete Adjuvant. High titres of immunospecific IgY antibodies against human IgG were recorded in the eggs from the chickens immunised orally with the antigen combined with glutaraldehyde conjugated CTB and in the chickens immunised with the antigen combined with Softigen. The present results show that invasive technique related stress could be eliminated/reduced in polyclonal antibody producing animals.
authors.affiliation: Department of Physiology, Division of Comparative Medicine, Uppsala University BMC, Box 572, 751 23 Uppsala, Sweden.
authors.forename: G P
authors.initials: GP
authors.lastname: Hedlund
authors.forename: J
authors.initials: J
authors.lastname: Hau
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000284:Administration, Oral; D000827:Animal Welfare; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D002645:Chickens / Q000276:immunology*; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000276:immunology; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical; D004527:Egg Proteins / Q000276:immunology*; D004530:Egg Yolk / Q000276:immunology*; D005260:Female; D005976:Glutaral / Q000494:pharmacology; D005989:Glycerides / Q000008:administration & dosage* / Q000276:immunology; D006801:Humans; D007114:Immunization / Q000662:veterinary*; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D019344:Lactic Acid / Q000008:administration & dosage; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D003432:Cross-Linking Reagents; D004527:Egg Proteins; D005989:Glycerides; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C451979:softigen; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C015831:dimethyldioctadecylammonium; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002772:Cholera Toxin; D005976:Glutaral