Regulation of NaK-ATPase by Platelet-Derived Growth Factors in Cultured Rat Thoracic Aortic Smooth Muscle Cells.
Regulation of (Na(+) + K(+))-adenosine triphosphatase (NaK-ATPase) by platelet-derived growth factor (PDGF) in cultured rat thoracic aortic smooth muscle cells (SMC) was examined. PDGF-BB enhances SMC proliferation and NaK-ATPase activity. Ouabain, an inhibitor of NaK-ATPase activity, prevents PDGF-BB-induced SMC proliferation. As shown by Western blot and immunochemiluminescence analysis, PDGF-BB also enhances alpha(1), truncated alpha(1), and beta(1) NaK-ATPase subunit levels. PDGF-AA and PDGF-AB show no effect on alpha(1) and truncated alpha(1) levels in slot blot analysis. Induction of NaK-ATPase subunit levels by PDGF-BB could be one of the initial events in vascular SMC proliferation. Copyright 1996 S. Karger AG, Basel
Lo
C.-S.
CS
Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, Md., USA.