Direct chiral resolution of tartaric acid in food products by ligand exchange capillary electrophoresis using copper(II)-D-quinic acid as a chiral selector.
Chiral resolution of native DL-tartaric acid was performed by ligand-exchange capillary electrophoresis using copper(II)-D-quinic acid as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of tartaric acid were studied. The running conditions for optimum separation of tartaric acid were found to be 1 mM copper(II) sulfate-10 mM D-quinic acid (pH 5.0) with an effective voltage of -15 kV at 30 degrees C, using direct detection at 250 nm, and resolution of racemic tartaric acid was approximately 1.3. With this system, chiral resolution of DL-tartaric acid in food products was conducted successfully.
Kodama
S
S
Toyama Institute of Health, Kosugi-machi, Japan. lee07664@nifty.ne.jp
Yamamoto
A
A
Matsunaga
A
A
Hayakawa
K
K
D003300:Copper / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005504:Food Analysis; D008024:Ligands; D011801:Quinic Acid / Q000737:chemistry*; D013237:Stereoisomerism; D013644:Tartrates / Q000032:analysis*
D008024:Ligands; D013644:Tartrates; D011801:Quinic Acid; D003300:Copper; C029768:tartaric acid