title: Rules of chemokine receptor association with T cell polarization in vivo.
abstract: Current concepts of chemokine receptor (CKR) association with Th1 and Th2 cell polarization and effector function have largely ignored the diverse nature of effector and memory T cells in vivo. Here, we systematically investigated the association of 11 CKRs, singly or in combination, with CD4 T cell polarization. We show that Th1, Th2, Th0, and nonpolarized T cells in blood and tissue can express any of the CKRs studied but that each CKR defines a characteristic pool of polarized and nonpolarized CD4 T cells. Certain combinations of CKRs define populations that are markedly enriched in major subsets of Th1 versus Th2 cells. For example, although Th0, Th1, and Th2 cells are each found among blood CD4 T cells coordinately expressing CXCR3 and CCR4, Th1 but not Th2 cells can be CXCR3(+)CCR4(-), and Th2 but only rare Th1 cells are CCR4(+)CXCR3(-). Contrary to recent reports, although CCR7(-) cells contain a higher frequency of polarized CD4 T cells, most Th1 and Th2 effector cells are CCR7(+) and thus may be capable of lymphoid organ homing. Interestingly, Th1-associated CKRs show little or no preference for Th1 cells except when they are coexpressed with CXCR3. We conclude that the combinatorial expression of CKRs, which allow tissue- and subset-dependent targeting of effector cells during chemotactic navigation, defines physiologically significant subsets of polarized and nonpolarized T cells.
authors.affiliation: Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University School of Medicine, Stanford, California, USA. chkim@stanford.edu
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: L
authors.initials: L
authors.lastname: Rott
authors.forename: E J
authors.initials: EJ
authors.lastname: Kunkel
authors.forename: M C
authors.initials: MC
authors.lastname: Genovese
authors.forename: D P
authors.initials: DP
authors.lastname: Andrew
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: E C
authors.initials: EC
authors.lastname: Butcher
mesh_terms: D015496:CD4-Positive T-Lymphocytes; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007156:Immunologic Memory; D010641:Phenotype; D011485:Protein Binding; D054400:Receptors, CCR7; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4 / Q000378:metabolism; D019707:Receptors, Chemokine / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*; D018417:Th1 Cells; D018418:Th2 Cells / Q000378:metabolism
chemical_list: C515370:CCR7 protein, human; C515187:CXCR3 protein, human; D016207:Cytokines; D054400:Receptors, CCR7; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine