Cellular solid-phase binding assay and mass spectrometry for screening of alpha 4 beta 7 integrin antagonists.
A qualitative cellular solid-phase binding assay for screening alpha 4 beta 7 integrin antagonists attached via photolinker to TentaGel Macrobeads has been developed. An activation of the integrins with Mn(2+) was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.
Gottschling
D
D
Institut für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
Boer
J
J
Schuster
A
A
Holzmann
B
B
Kessler
H
H
D002448:Cell Adhesion; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006801:Humans; D016023:Integrins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D014407:Tumor Cells, Cultured
D016023:Integrins; C071777:integrin alpha4beta7