IMPAN cells: a pancreatic model for differentiation into endocrine cells.
It is currently believed that pancreatic progenitor or stem cells exist in the ductal cell population and that these cells have the ability to be grown and differentiated into endocrine cells for the treatment of diabetes. In this study, we have examined this potential in IMPAN (Immortalized Pancreatic) cells. These cells are derived from the adult H-2K(b)-tsA58 transgenic mouse. We observed an increased mRNA expression of insulin, proendocrine gene neurogenin 3, and beta-cell transcription factor Pdx1 when the cells were grown on bovine collagen I gels. The induction profile of these three genes was similar under the tested conditions. No glucagon or other endocrine-specific transcription factors were detectable. Application of GIP, GLP-1 derivative NN2211, and activin-A/betacellulin to IMPAN cells in normal culture did not lead to endocrine differentiation. In conclusion, it appears that the ability of IMPAN cells to mature to endocrine cells is limited.
Klein
T
T
Department of Developmental Biology, Hagedorn Research Institute, Niels Steensensvej 6, DK-2820 Gentofte, Denmark.
Frandsen
U
U
Heller
R S
RS
Serup
P
P
D028341:Activins / Q000494:pharmacology; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D004702:Endocrine Glands / Q000166:cytology*; D005934:Glucagon / Q000031:analogs & derivatives / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1 / Q000031:analogs & derivatives; D018398:Homeodomain Proteins; D028322:Inhibin-beta Subunits / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis; D000069450:Liraglutide; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology; D013696:Temperature; D015534:Trans-Activators / Q000096:biosynthesis
D028341:Activins; D051792:Basic Helix-Loop-Helix Proteins; D003094:Collagen; D024042:Collagen Type I; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D018398:Homeodomain Proteins; D028322:Inhibin-beta Subunits; D007328:Insulin; D000069450:Liraglutide; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; C407737:Neurog3 protein, mouse; C422405:activin A; C084010:pancreatic and duodenal homeobox 1 protein