[Analysis of immunophenotype of hematopoietic stem/progenitor cells in human umbilical cord blood with flow cytometry and its significance].
OBJECTIVE
To study the difference of immunophenotype of hematopoietic stem/progenitor cells (HSPCs) between human umbilical cord blood(UCB) and bone marrow(BM).


METHODS
The expression of CD antigens and cell adhesion molecules on HSPCs in 38 UCB and 10 cases BM were investigated with monoclonal antibodies and double immunofluorescence using flow cytometry.


RESULTS
1. The CD34+ cell population in nucleated cells of UCB was similar to that of BM, being about 0.5%; 2. The frequencies of CD34+CD38-, CD34+HLA-DR- and CD34+H-CAM+ (CD44+) cells in UCB CD34+ cells were higher, while the frequencies of CD34+CD13+ and CD34+CD19+ cells in UCB were lower than that in BM.


CONCLUSIONS
The total number of CD34+ cells in UCB and BM was very similar. However, there was a higher proportion of more primary HSPCs in UCB, which was able to reconstitute hematopoiesis as an potential source of HSPCs, the proportion of relatively mature HSPCs (CD34+ CD19+ and CD34+CD13+) were less in UCB than in BM, which probably accounted for the relatively slow haematopoietic reconstitution following UCB transplantation.
Guo
R
R
Department of Hematology, First Affiliated Hospital, Henan Medical University, Zhengzhou 450052, China.
Ma
B
B
Zou
D
D
D018952:Antigens, CD34 / Q000032:analysis; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D013234:Stem Cells / Q000276:immunology*
D018952:Antigens, CD34