Changes in surface charge of low-density lipoproteins during oxidative modification.
The negative surface charge of low-density lipoproteins increased during their oxidative modification induced by autooxidation at 37 degrees C. The degree of changes depended on the time of autooxidation: the surface charge remained practically unchanged after short-term oxidation (6-h incubation), but then progressively increased and after 24-h oxidation it 2-fold surpassed the initial level. Long-term incubation of low-density lipoproteins in the presence of EDTA inhibiting lipid peroxidation did not change their surface charge. These changes probably contribute to atherogenic activity of oxidized low-density lipoproteins. The degree of oxidative modification of low-density lipoproteins was precisely estimated using fluorescence probes.
Aidyraliev
R K
RK
National Center of Cardiology and Therapy, Kirghiz Ministry of Health, Bishkek. cardio@elcat.kg
Azizova
O A
OA
Mirrakhimov
M M
MM
Lopukhin
Y M
YM
D000838:Anions; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000737:chemistry* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D011487:Protein Conformation; D013499:Surface Properties
D000838:Anions; D002614:Chelating Agents; D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; D008315:Malondialdehyde; D004492:Edetic Acid