Quantification of doxorubicin in plasma--a comparative study of capillary and venous blood sampling.
Doxorubicin, an anthraquinone glycoside, is currently one of the clinically most important antineoplastic drugs. The aim of the present study was to identify potential concentration differences of doxorubicin in plasma from capillary and venous blood samples. Sixteen patients (seven females and nine males; median age: 37 years, range: 1-77 years) were included. The quantitative analysis of doxorubicin was carried out by reversed-phase liquid chromatography with fluorometric detection. The concentration of doxorubicin in capillary and venous samples were closely correlated (r=0.98; p<0.0001). The median plasma concentration ratio capillary/venous was 1.13 (95% confidence interval: 1.06-1.20) and not affected either by plasma drug concentration, age or the body mass index of the patient. The concentration ratio was significantly higher in males (median: 1.18) than in females (median: 1.01). The observed concentration differences of doxorubicin in plasma from capillary and venous samples are of minor importance only. Capillary blood sampling is recommended for use in pharmacokinetic studies of doxorubicin, especially in pediatric patients, in order to avoid sometimes traumatic venous blood sampling.
Palm
C
C
Karolinska Pharmacy, Karolinska Hospital, 171 76 Stockholm, Sweden.
Björk
O
O
Björkholm
M
M
Eksborg
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D001800:Blood Specimen Collection; D002196:Capillaries / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D014680:Veins / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; C010013:adriamycinol