title: Free cytosolic Ca2+ recordings from myenteric neurones in multilayer intestinal preparations.
abstract: The ability to simultaneously monitor different myenteric neurones in a multilayer preparation may enhance our understanding of the enteric nervous system. Longitudinal muscle myenteric plexus preparations were mounted in recording chambers with a coverslip base and loaded with Indo-1-AM. cytosolic Ca2+ concentration ([Ca2+]i); changes were recorded at room temperature with a confocal microscope. In addition to mechanical (pressure-ring) and pharmacological (nifedipine) reduction of muscle contractions, purpose-designed software was developed to reposition regions of interest and avoid artefacts. Confocal scanning permitted optical selection of single cell layers. High K+ depolarization, used to distinguish between excitable and nonexcitable cells, caused a synchronous [Ca2+]i rise in 84.3% of the ganglion cells. Acetylcholine, substance P and serotonin (all at 10(-5) mol L(-1)) induced transient [Ca2+]i changes in subpopulations of myenteric neurones (45.1%, 42.9 and 21.9%, respectively). In addition to immediate responses to agonists, delayed [Ca2+]i changes were also recorded, suggesting the presence of both directly activated and synaptically driven neurones. Functionally identified neurones and other cells in close apposition to the ganglia (interstitial cells of Cajal) could also be studied. This study demonstrates the potential of optical Ca2+ recordings to monitor spread of activity in myenteric neurones and to study their interaction with non-neuronal targets.
authors.affiliation: Center for Gastroenterological Research, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Vanden Berghe
authors.forename: L
authors.initials: L
authors.lastname: Missiaen
authors.forename: E
authors.initials: E
authors.lastname: Bellon
authors.forename: J M
authors.initials: JM
authors.lastname: Vanderwinden
authors.forename: J
authors.initials: J
authors.lastname: Janssens
authors.forename: J
authors.initials: J
authors.lastname: Tack
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002232:Carbocyanines; D003600:Cytosol / Q000378:metabolism; D004594:Electrophysiology; D005260:Female; D005456:Fluorescent Dyes; D016166:Free Radical Scavengers / Q000494:pharmacology; D006168:Guinea Pigs; D007211:Indoles; D007422:Intestines / Q000294:innervation; D008297:Male; D018613:Microscopy, Confocal; D009046:Motor Neurons / Q000378:metabolism*; D009130:Muscle, Smooth / Q000294:innervation; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011312:Pressure; D012701:Serotonin / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: C035005:3,3'-dihexadecylindocarbocyanine; D002121:Calcium Channel Blockers; D002232:Carbocyanines; D005456:Fluorescent Dyes; D016166:Free Radical Scavengers; D007211:Indoles; D014665:Vasodilator Agents; D012701:Serotonin; D013373:Substance P; D009543:Nifedipine; C048960:indo-1; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium