Hydrolysis of linear DNA duplex catalyzed by Co(III) complex of cyclen attached to polystyrene.
To design artificial restriction enzymes, synthetic catalytic centers that effectively hydrolyze linear double-stranded polydeoxyribonucleotides are needed. The Co(III) complex of cyclen (CoCyc) attached to polystyrene derivatives hydrolyzes linearized pUC18 DNA with half-lives as short as 30 min at 25 degrees C. The catalytic activity of CoCyc is enhanced by >150 times on attachment to the resin.
Jeung
C S
CS
Artzyme Biotech Corporation, 403-1, Silim-2-Dong, Seoul 151-012, Republic of Korea.
Song
J B
JB
Kim
Y H
YH
Suh
J
J
D002384:Catalysis; D003035:Cobalt / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*
D009942:Organometallic Compounds; D011137:Polystyrenes; C442399:cyclencobalt(III); D003035:Cobalt; D004247:DNA