Automated ribotyping and random amplified polymorphic DNA analysis for molecular typing of Salmonella enteritidis and Salmonella typhimurium strains isolated in Italy.
OBJECTIVE
The ability of automated ribotyping and random amplified polymorphic DNA (RAPD) analysis to differentiate Salmonella enteritidis and Salmonella typhimurium isolates in relation to their origin was evaluated.


RESULTS
The restriction enzymes EcoRI, PvuII and PstI, and the random primers OPB17 and P1254, were tested for ribotyping and RAPD analysis, respectively. Seventeen subtypes were identified among the isolates of the two pathogenic Salmonella serovars using the RiboPrinter, and 25 subtypes using RAPD.


CONCLUSIONS
The greatest degree of genetic diversity was observed among Salm. typhimurium isolates using both automated ribotyping (Simpson's index of discrimination 0878) and RAPD (Simpson's index of discrimination 0886).


CONCLUSIONS
According to the results of this research, automated ribotyping and RAPD are two useful genotyping techniques for identifying unique and common subtypes associated with a specific source and location, and provide powerful tools for epidemiological investigations.
De Cesare
A
A
Dipartimento di Scienze degli Alimenti, University of Bologna, Italy. decesare@alma.unibo.it
Manfreda
G
G
Dambaugh
T R
TR
Guerzoni
M E
ME
Franchini
A
A
D000818:Animals; D015373:Bacterial Typing Techniques; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis; D007558:Italy / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D012481:Salmonella Infections, Animal / Q000453:epidemiology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014422:Turkeys
D004269:DNA, Bacterial