Induction of endogenous beta-galactosidase by ionizing radiation complicates the analysis of p53-LacZ transgenic mice.
Studies of the response of p53-lacZ transgenic mice have uncovered an unexpected induction of endogenous acid-beta-galactosidase activity following whole body irradiation. Strong induction of endogenous enzyme activity is seen in a variety of mouse strains commonly used in the production of transgenes. The induction of endogenous enzyme activity therefore complicates the analysis of p53-lacZ transgenes and may also influence the analysis of radiation responses in other lacZ-reporter mice.
Coates
P J
PJ
Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, UK.
Lorimore
S A
SA
Rigat
B A
BA
Lane
D P
DP
Wright
E G
EG
D000818:Animals; D016477:Artifacts; D002021:Buffers; D004790:Enzyme Induction / Q000528:radiation effects; D029968:Escherichia coli Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D005189:False Positive Reactions; D017930:Genes, Reporter; D005813:Genes, Synthetic; D016158:Genes, p53; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D008247:Lysosomes / Q000201:enzymology*; D008262:Macrophage Activation / Q000528:radiation effects; D008264:Macrophages / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D010587:Phagocytosis; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000096:biosynthesis*; D012469:Salivary Glands / Q000201:enzymology; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology; D019076:Transgenes; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D014916:Whole-Body Irradiation; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D002021:Buffers; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D016159:Tumor Suppressor Protein p53; D001616:beta-Galactosidase