Different effects of genistein and resveratrol on oxidative DNA damage in vitro.
Previous studies have demonstrated that phenolic compounds, including genistein (4',5,7-trihydroxyisoflavone) and resveratrol (3,4',5-trihydroxystilbene), are able to protect against carcinogenesis in animal models. This study was undertaken to examine the ability of genistein and resveratrol to inhibit reactive oxygen species (ROS)-mediated strand breaks in phi X-174 plasmid DNA. H(2)O(2)/Cu(II) and hydroquinone/Cu(II) were used to cause oxidative DNA strand breaks in the plasmid DNA. We demonstrated that the presence of genistein at micromolar concentrations resulted in a marked inhibition of DNA strand breaks induced by either H(2)O(2)/Cu(II) or hydroquinone/Cu(II). Genistein neither affected the Cu(II)/Cu(I) redox cycle nor reacted with H(2)O(2) suggest that genistein may directly scavenge the ROS that participate in the induction of DNA strand breaks. In contrast to the inhibitory effects of genistein, the presence of resveratrol at similar concentrations led to increased DNA strand breaks induced by H(2)O(2)/Cu(II). Further studies showed that in the presence of Cu(II), resveratrol, but not genistein was able to cause DNA strand breaks. Moreover, both Cu(II)/Cu(I) redox cycle and H(2)O(2) were shown to be critically involved in resveratrol/copper-mediated DNA strand breaks. The above results indicate that despite their similar in vivo anticarcinogenic effects, genistein and resveratrol appear to exert different effects on oxidative DNA damage in vitro.
Win
William
W
Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, St. Albert Hall, St. John's University, 8000 Utopia Parkway, Jamaica, NY 11439, USA.
Cao
Zhuoxiao
Z
Peng
Xingxiang
X
Trush
Michael A
MA
Li
Yunbo
Y
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D003300:Copper / Q000378:metabolism; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D019833:Genistein / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity; D006873:Hydroquinones / Q000633:toxicity; D010084:Oxidation-Reduction; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D006873:Hydroquinones; D013267:Stilbenes; D003300:Copper; D004247:DNA; D006861:Hydrogen Peroxide; D019833:Genistein; D000077185:Resveratrol; C031927:hydroquinone