An antibody against anemia-inducing substance inhibits cation influx in red blood cells from cancer patients.
Resealed ghost of red blood cells (RBCs) from advanced cancer patients includes proteins with antigenicities common to an anemia-inducing substance that we separated from plasma of patients with advanced malignant neoplasms. Although cation influx in vitro in RBCs from a cancer patient is larger than that from a normal healthy volunteer, an antibody against anemia-inducing substance inhibited the in vitro cation influx in RBCs from the cancer patient. Activation of the cation influx with N-ethylmaleimide after reaction with the antibody reversed the effect to create a greater cation influx in RBCs from the patient, as compared with that from the healthy volunteer.
Honda
K
K
Department of Obstetrics and Gynecology, Osaka City University Graduate School of Medicine, Abeno-ku, Osaka 5454-8585, Japan.
Ishiko
O
O
Ogita
S
S
D000740:Anemia / Q000139:chemically induced; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D001798:Blood Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D002412:Cations / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D006801:Humans; D008970:Molecular Weight; D009369:Neoplasms / Q000097:blood*; D010051:Ovarian Neoplasms / Q000097:blood; D012413:Rubidium / Q000378:metabolism
D000906:Antibodies; D001798:Blood Proteins; D002412:Cations; D012413:Rubidium; D005033:Ethylmaleimide