title: A transcriptional roadmap to wood formation.
abstract: The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of wood-forming tissues allowed us to determine a unique tissue-specific transcript profile for a well defined developmental gradient. RNA was prepared from different developmental stages of xylogenesis for DNA microarray analysis by using a hybrid aspen unigene set consisting of 2,995 expressed sequence tags. The analysis revealed that the genes encoding lignin and cellulose biosynthetic enzymes, as well as a number of transcription factors and other potential regulators of xylogenesis, are under strict developmental stage-specific transcriptional regulation.
authors.affiliation: Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Hertzberg
authors.forename: H
authors.initials: H
authors.lastname: Aspeborg
authors.forename: J
authors.initials: J
authors.lastname: Schrader
authors.forename: A
authors.initials: A
authors.lastname: Andersson
authors.forename: R
authors.initials: R
authors.lastname: Erlandsson
authors.forename: K
authors.initials: K
authors.lastname: Blomqvist
authors.forename: R
authors.initials: R
authors.lastname: Bhalerao
authors.forename: M
authors.initials: M
authors.lastname: Uhlén
authors.forename: T T
authors.initials: TT
authors.lastname: Teeri
authors.forename: J
authors.initials: J
authors.lastname: Lundeberg
authors.forename: B
authors.initials: B
authors.lastname: Sundberg
authors.forename: P
authors.initials: P
authors.lastname: Nilsson
authors.forename: G
authors.initials: G
authors.lastname: Sandberg
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D002482:Cellulose / Q000096:biosynthesis; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008031:Lignin / Q000096:biosynthesis; D018519:Meristem / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D014158:Transcription, Genetic; D014197:Trees / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D014934:Wood
chemical_list: D002482:Cellulose; D008031:Lignin