Preserving the original heparin structure of a novel low molecular weight heparin by gamma-irradiation.
Several preparations of low molecular weight heparins (LMWHs) obtained by physical depolymerisation (irradiation with gamma-rays) of pig mucosal heparin have been characterised by mono- and two-dimensional NMR spectroscopy. Integration of typical 1H- and 13C-NMR signals provided a useful quantification of their sulfation pattern. The availability of the corresponding parent heparins showed that the original structure (including that of the active site for antithrombin, as also confirmed by affinity chromatography) had not been significantly modified by the depolymerisation procedure. This process involves only a slight decrease of undersulfated sequences. A peculiarity of gamma-LMWH is the absence of the 'linkage region', commonly present in unmodified heparins and most LMWHs.
Bisio
A
A
G. Ronzoni Institute for Chemical and Biochemical Research, Milan, Italy. bisio@ronzoni.it
De Ambrosi
L
L
Gonella
S
S
Guerrini
M
M
Guglieri
S
S
Maggia
G
G
Torri
G
G
D000818:Animals; D002846:Chromatography, Affinity; D015951:Factor Xa / Q000737:chemistry; D005720:Gamma Rays; D005753:Gastric Mucosa / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry* / Q000528:radiation effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D015203:Reproducibility of Results; D013552:Swine
D006495:Heparin, Low-Molecular-Weight; D015951:Factor Xa