Gene sll0033 from Synechocystis 6803 encodes a carotene isomerase involved in the biosynthesis of all-E lycopene.
The function of gene sll0033 from Synechocystis 6803 which is homologous to the bacterial crtI-type phytoene desaturase genes was elucidated as a novel carotene isomerase. Escherichia coli transformed with all genes necessary for the formation of zeta-carotene and expressing a zeta-carotene desaturase synthesized the positional isomer prolycopene (7,9,7',9'Z lycopene) which cannot be cyclized in the subsequent reactions to a- and beta-carotene. Upon cotransformation with sll0033, the formation of all-E lycopene is mediated instead.
Breitenbach
J
J
Biosynthesis Group, Botanical Institute, J.W. Goethe Universität, Frankfurt, Germany.
Vioque
A
A
Sandmann
G
G
D000975:Antioxidants / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial; D007535:Isomerases / Q000235:genetics*; D000077276:Lycopene; D019745:cis-trans-Isomerases / Q000235:genetics*
D000975:Antioxidants; D002338:Carotenoids; D007535:Isomerases; D019745:cis-trans-Isomerases; D000077276:Lycopene