Characterisation of a cDNA encoding chick eukaryotic translation initiation factor-2 beta.
A full length cDNA for the beta subunit of chick (Gallus gallus) eukaryotic translation initiation factor-2 is described. This cDNA was isolated by screening a chick cDNA library with a probe derived via differential display of developing chick heart tissue. Up-regulated expression of eIF-2 beta mRNA was confirmed by reverse Northern dot blot analysis. eIF-2 beta, together with eIF-2 alpha and eIF-2 gamma, comprise subunits of a complex that promotes the binding of methionyl-tRNA to ribosomes during the initiation of protein translation. The nucleotide sequence of the chick eIF-2 beta cDNA predicts a protein of 334 amino acids that has 95%, 93%, 56% and 37% sequence identity with rabbit, human, drosophila and yeast eIF-2 beta, respectively. The deduced eIF-2 beta protein contains a number of functional motifs and domains consistent with the putative function of this protein; these include a potential C2-C2 zinc-finger binding domain, three polylysine regions, and three acidic regions.
Sneesby
K J
KJ
School of Biomolecular and Biomedical Science, Griffith University, Nathan, Brisbane, Queensland 4111, Australia.
Crane
D I
DI
Murrell
W G
WG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D018076:DNA, Complementary / Q000235:genetics*; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
D018076:DNA, Complementary; D015852:Eukaryotic Initiation Factor-2