Short-term exposure to dietary Pb and/or DMSA affects dopamine and dopamine metabolite levels in the medulla, optic tectum, and cerebellum of rainbow trout (Oncorhynchus mykiss).
Rainbow trout (Oncorhynchus mykiss) were randomly assigned to one of the following dietary exposure conditions: lead (Pb) solvent (2% nitric acid), meso-2,3-dimercaptosuccinic acid (DMSA) solvent (0.1 N NaOH), Pb, DMSA, Pb followed by Pb solvent, or Pb followed by DMSA. Medulla, cerebellum, and optic tectum homogenates were analyzed for dopamine (DA), homovanillic acid (HVA), and 3,4-dihydroxyphenylacetic acid (DOPAC). DA levels in all brain regions tended to be highest for trout exposed to dietary Pb followed by dietary DMSA. DA levels were elevated for trout exposed to dietary DMSA and Pb followed by Pb solvent. DA levels were below control levels for trout exposed to Pb only. HVA levels varied across brain regions. However, HVA levels in all brain regions tended to be elevated for trout exposed to dietary DMSA and Pb followed by Pb solvent. DOPAC levels across all brain regions were below control levels for trout dietary exposed to DMSA, Pb only, Pb followed by Pb solvent, and Pb followed by DMSA. These data indicate that Pb and/or DMSA have the potential of altering DA, HVA, and DOPAC levels in the medulla, cerebellum, and optic tectum. The animal model of short-term dietary exposure to Pb and DMSA, both alone and sequentially, to mimic dietary exposure to Pb and the oral delivery of DMSA, that our laboratory has developed, may be useful in future studies aimed at characterizing the neurobiological mechanisms by which Pb and/or DMSA alter neurotransmitter levels and behavior.
Rademacher
D J
DJ
Department of Psychology, University of Wisconsin-Milwaukee, 224 Garland Hall, 2441 East Hartford Avenue, Milwaukee, WI 53211, USA.
Steinpreis
R E
RE
Weber
D N
DN
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D003530:Cyprinidae; D004032:Diet; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D008526:Medulla Oblongata / Q000187:drug effects* / Q000378:metabolism; D017686:Oncorhynchus mykiss; D004113:Succimer / Q000008:administration & dosage*; D013477:Superior Colliculi / Q000187:drug effects* / Q000378:metabolism
D002614:Chelating Agents; D015102:3,4-Dihydroxyphenylacetic Acid; D007854:Lead; D004113:Succimer; D004298:Dopamine; D006719:Homovanillic Acid