title: Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene.
abstract: A recombinant porcine adenovirus expressing the classical swine fever virus (CSFV) gp55/E2 gene was administered to commercially available pigs via oral or subcutaneous routes and their susceptibility to oral and subcutaneous challenge with CSFV was determined. 100% of animals vaccinated and challenged subcutaneously were protected. In the groups of pigs vaccinated either orally or subcutaneously and then challenged orally, 60% of animals were protected. Before challenge, neutralising antibodies to CSFV were detected in 60% of pigs vaccinated subcutaneously, but in none of those given the vaccine orally. CSFV antigen was found in the spleens of surviving pigs that had been vaccinated orally. In contrast, subcutaneous vaccination was shown to preclude the presence of CSFV in the spleen of animals that survived challenge.
authors.affiliation: CSIRO, Australian Animal Health Laboratory, Geelong, Victoria.
authors.forename: J M
authors.initials: JM
authors.lastname: Hammond
authors.forename: E S
authors.initials: ES
authors.lastname: Jansen
authors.forename: C J
authors.initials: CJ
authors.lastname: Morrissy
authors.forename: M M
authors.initials: MM
authors.lastname: Williamson
authors.forename: A L
authors.initials: AL
authors.lastname: Hodgson
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
mesh_terms: D025662:Adenoviruses, Porcine / Q000235:genetics*; D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D009500:Neutralization Tests; D011995:Recombination, Genetic; D013552:Swine; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus