In vitro selection of RNA aptamers carrying multiple biotin groups in the side chains.
In vitro selection or the systematic evolution of ligands by an exponential enrichment (SELEX) technique using a biotin-carrying nucleotide monomer was used for the development of a new type of molecular recognition sensor. Cytidine triphosphate (CTP) carrying the biotinyl group at the N4-position was applied for the technique. A pool of random sequence RNAs containing biotinyl groups in the side chains was prepared, and the RNAs binding to adenosine 5'-triphosphate (ATP) were selected. The selected nonnatural RNAs were used for an assay of ATP. Since they carried multiple biotin groups in the side chains, the sensitivity was high.
Ito
Y
Y
Departmentof Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Tokushima 770-8506, Japan. ito@bio.tokushima-u.ac.jp
Suzuki
A
A
Kawazoe
N
N
Imanishi
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation / Q000379:methods; D004587:Electrophoresis, Agar Gel; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012680:Sensitivity and Specificity
D005456:Fluorescent Dyes; D012313:RNA; D001710:Biotin; D000255:Adenosine Triphosphate