title: Biological clock in total darkness: the Clock/MOP3 circadian system of the blind subterranean mole rat.
abstract: Blind subterranean mole rats retain a degenerated, subcutaneous, visually blind but functionally circadian eye involved in photoperiodic perception. Here we describe the cloning, sequence, and expression of the circadian Clock and MOP3 cDNAs of the Spalax ehrenbergi superspecies in Israel. Both genes are relatively conserved, although characterized by a significant number of amino acid substitutions. The glutamine-rich area of Clock, which is assumed to function in circadian rhythmicity, is expanded in Spalax compared with that of humans and mice, and is different in amino acid composition from that of rats. We also show that MOP3 is a bona fide partner of Spalax Clock and that the Spalax Clock/MOP3 dimer is less potent than its human counterpart in driving transcription. We suggest that this reduction in transcriptional activity may be attributed to the Spalax Clock glutamine-rich domain, which is unique in its amino acid composition compared with other studied mammalian species. Understanding Clock/MOP3 function could highlight circadian mechanisms in blind mammals and their unique pattern as a result of adapting to life underground.
authors.affiliation: Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Avivi
authors.forename: U
authors.initials: U
authors.lastname: Albrecht
authors.forename: H
authors.initials: H
authors.lastname: Oster
authors.forename: A
authors.initials: A
authors.lastname: Joel
authors.forename: A
authors.initials: A
authors.lastname: Beiles
authors.forename: E
authors.initials: E
authors.lastname: Nevo
mesh_terms: D056930:ARNTL Transcription Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D001683:Biological Clocks / Q000502:physiology*; D001766:Blindness / Q000235:genetics*; D056926:CLOCK Proteins; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary; D003624:Darkness; D019281:Dimerization; D015870:Gene Expression; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D019577:Mole Rats; D008969:Molecular Sequence Data; D015534:Trans-Activators / Q000145:classification / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D056930:ARNTL Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D056926:CLOCK Proteins; D018076:DNA, Complementary; D015534:Trans-Activators; D014157:Transcription Factors; C538960:BMAL1 protein, human; C538961:Bmal1 protein, mouse; C538943:CLOCK protein, human; C538944:Clock protein, mouse; C538945:Clock protein, rat