Distribution of stanniocalcin 1 in rat kidney and its regulation by vitamin D3.
Stanniocalcin is a glycoprotein hormone first described in fish as a hypocalcemic factor, and recently its mammalian counterpart has been identified. Localization of stanniocalcin 1 and its regulation of expression were determined in control and 1alpha,25-dihydroxyvitamin D3-treated rats. Immunoreactivity for stanniocalcin 1 was detected in the loop of Henle, macula densa cells, distal convoluted tubule (DCT), and cortical collecting duct (CCD), and also faintly in the medullary collecting ducts. Pre-embedding electron-microscopic immunocytochemistry revealed stanniocalcin 1 in the apical membrane of cells of loop of Henle, DCT, and principal cells of CCD. The expression of stanniocalcin 1 was increased by elevated plasma calcium via 1alpha,25-dihydroxyvitamin D3 treatment. In conclusion, stanniocalcin 1 was expressed in the apical membrane of distal nephron segments and enhanced by vitamin D3.
Ookata
K
K
Division of Nephrology and Endocrinology, University of Tokyo, Japan.
Tojo
A
A
Onozato
M L
ML
Kashiwagi
M
M
Honda
S
S
Hirose
S
S
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D006023:Glycoproteins / Q000378:metabolism*; D006728:Hormones / Q000378:metabolism*; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D002120:Calcium Channel Agonists; D006023:Glycoproteins; D006728:Hormones; C051795:teleocalcin; D002117:Calcitriol