Exon 2 sequence analysis of a novel HLA-DRB1 allele, DRB1*0314.
We describe the identification of a new DRB1*03 variant in a Caucasian volunteer bone-marrow donor (DR'KW'). The indication for the existence of this new variant arose from contradictory results obtained by serological and several DNA typing methods (SSP, SSOP). Analysis of the exon 2 sequence revealed that the DRB1*0314 contained the characteristic DR3 specific sequence motifs, except for codon 77 (AAC-->ACC) where threonine is substituted for asparagine.
Koninkx
H J
HJ
Department of Blood Transfusion and Transplantation Immunology, University Medical Center Nijmegen, 6500 NB Nijmegen, The Netherlands.
Tijssen
H J
HJ
Joosten
I
I
D000483:Alleles; D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D003062:Codon; D005091:Exons; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013912:Threonine / Q000235:genetics; D014019:Tissue Donors
D003062:Codon; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D013912:Threonine