[Decreased stimulation of glycosaminoglycan synthesis by human skin fibroblasts by interleukin 6 within the scope of in vitro aging].
Addition of human recombinant interleukin 6 (IL-6) to culture medium (supplemented MEM without or with 10% fetal calf serum (FCS)) of human skin fibroblasts exerted a stimulating effect in a dose-dependent manner on glycosaminoglycan (GAG) synthesis, including hyaluronic acid (hyaluronan) synthesis, of young (phase-II) skin fibroblasts in concentrations of 1 ng/ml and 10 ng/ml. Stimulation was mainly due to an increase in extracellular GAGs (secreted into culture medium), and to a lesser extent to an increase in peri- and intracellular GAGs. Stimulation with 1 ng/ml and 10 ng/ml IL-6 led to an increase in hyaluronic acid from 48% to 61% (-FCS) and from 77% to 90% (+10% FCS), respectively. Maximum stimulation, with and without FCS, was achieved by 10 ng/ml IL-6. Compared to young (phase-II) cells, senescent (phase-III) cells, showed no significant stimulation of total GAG (including hyaluronic acid) synthesis by IL-6. The diminished response of GAG- and hyaluronic acid synthesis during aging of these in vitro cultured fibroblasts should motivate further research if similar processes occur during aging in an organism.
Biecker
E
E
Institut für Physiologische Chemie Philipps-Universität Karl-von-Frisch-Str. 1 35033 Marburg, Germany.
Schachtschabel
D O
DO
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D015850:Interleukin-6 / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology*
D006025:Glycosaminoglycans; D015850:Interleukin-6; D011994:Recombinant Proteins; D006820:Hyaluronic Acid