An up-regulation of renal alpha(2)A-adrenoceptors is associated with resistance to salt-induced hypertension in Sabra rats.
This study investigates the incidence of high-salt diet in blood pressures, renal alpha(2)-adrenoceptor subtypes distribution, and gene expression in salt-sensitive (SBH) and salt-resistant (SBN) Sabra rats. Comparisons have been made between SBH and SBN rats submitted to a normal or a high-salt diet for 6 weeks. Only alpha(2)B-adrenoceptors are detected in kidneys of SBH rats, whatever the diet. In contrast, mRNA corresponding to alpha(2)A- and alpha(2)B-subtypes are found in this substrain. In these rats, high-salt diet increases blood pressures and up-regulates gene expression and density of only alpha(2)B-adrenoceptors. Inversely, alpha(2)A- and alpha(2)B-adrenoceptors and corresponding mRNA are found in kidneys of SBN rats. In these rats, a high-salt diet does not affect blood pressures but increases gene expression and densities of both alpha(2)A- and alpha(2)B-adrenoceptors. If the up-regulation of renal alpha(2)B-adrenoceptor subtypes is indicative of the hypertensive phenotype, the present study shows that this mechanism is also present in normotensive salt-resistant Sabra rats. In fact, the absence of alpha(2)A-adrenoceptors in SBH could be responsible for the lack of adequate receptor-mediated renal functions predisposing to salt-sensitivity and consequently the development of hypertension. Conversely, the presence of this receptor in SBN rats and its up-regulation could be protective change against the increase of alpha(2)B-adrenoceptors induced by the salt overload and could consequently be responsible for the resistance to salt-induced hypertension.
Khalid
M
M
Department of Biochemistry and Molecular Biology, Faculté de Médecine de Paris-Ouest, Université René Descartes, Paris, France.
Giudicelli
Y
Y
Dausse
J P
JP
D000818:Animals; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced / Q000209:etiology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics / Q000378:metabolism*; D012965:Sodium Chloride; D014018:Tissue Distribution; D015854:Up-Regulation
D018341:Receptors, Adrenergic, alpha-2; D012965:Sodium Chloride