title: The effects of different levels of dietary restriction on aging and survival in the Sprague-Dawley rat: implications for chronic studies.
abstract: A study was undertaken to determine the effects of incremental levels of dietary restriction (DR) in rats. Survival, growth, reproductive, and dietary intake (DI) variables were monitored in a chronic study in which male Sprague Dawley (SD) rats (NCTR colony) were fed their ration ad libitum (AL), or DR. The main objectives were to determine if low levels of DR could be used to increase the survival rate of SD rats in the chronic bioassay, and to identify the survival characteristics of a long-lived SD rat strain (NCTR colony). The average life span of AL rats was 115 months. At 104 weeks on study (110 weeks of age), the survival rate for the AL and 10%, 25%, and 40% DR groups was 63.4, 87.5, 87.5, and 97.5%, respectively. The largest increase in survival (24.1%) occurred between AL and 10% DR, indicating that very low levels of DR have a significant effect on survival. Whole-body, liver, prostate, and epididymis weights and body length were decreased by DR, whereas brain weight, testicular weight, and skull length were not altered by DR. Rats from the NCTR colony were found to be ideal for chronic studies because they are much longer-lived than other SD stocks. Although the 104-week survival rate for these SD, non-obese AL rats exceeds the FDA's "Redbook" survival guideline (> 50%) for chronic bioassays, the use of DR is advocated because it reduces individual variability in body weight.
authors.affiliation: Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA. pduffy@nctr.fda.gov
authors.forename: P H
authors.initials: PH
authors.lastname: Duffy
authors.forename: J E
authors.initials: JE
authors.lastname: Seng
authors.forename: S M
authors.initials: SM
authors.lastname: Lewis
authors.forename: M A
authors.initials: MA
authors.lastname: Mayhugh
authors.forename: A
authors.initials: A
authors.lastname: Aidoo
authors.forename: D G
authors.initials: DG
authors.lastname: Hattan
authors.forename: D A
authors.initials: DA
authors.lastname: Casciano
authors.forename: R J
authors.initials: RJ
authors.lastname: Feuers
mesh_terms: D000375:Aging / Q000502:physiology*; D000821:Animal Feed; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D002149:Energy Intake / Q000502:physiology*; D006257:Head / Q000033:anatomy & histology; D008099:Liver / Q000033:anatomy & histology; D008297:Male; D009929:Organ Size; D011467:Prostate / Q000033:anatomy & histology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction; D016019:Survival Analysis