[Tumour suppressor p53 gene sequence mutations in pleomorphic adenoma of salivary gland].
OBJECTIVE
To detect p53 exon 7 and 8 mutation and to study genesis and biological characteristics of pleomorphic adenoma of salivary galand.


METHODS
Polymerase chain reaction- single-strand conformational polymorphism (PCR-SSCP) and DNA sequencing analysis were used.


RESULTS
PCR-SSCP analysis showed that in 11 patients with positive SSCP, 7 showed abnormal exon 7 and 3 abnormal exon 8. Comparison of SSCP results with DNA sequencing showed that patients all patients with p53 exon 7 and 8 mutations were SSCP positive. Exon 7 mutation pattern was point mutation(T-->C). Exon 8 mutation pattern included base substitution (point mutation) (C-->A) and frame-shiftmutation (base insertion and base loss).


CONCLUSIONS
Polycodon and poly-pattern mutations appeared in p53 exon 7 and 8. Pleomorphic adenoma of salivary galand was similar to malignant tumor in p53 gene mutation. Poly-codon mutation could occur in the same case.
Wang
J
J
School of Stomatology, Hebei Medical University, Shijiazhuang 050017.
Dong
F
F
Yong
P
P
D008949:Adenoma, Pleomorphic / Q000235:genetics*; D005091:Exons / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D012468:Salivary Gland Neoplasms / Q000235:genetics*; D017422:Sequence Analysis, DNA