Determination of HCV genotype using two antibody assays and genome typing.
Two serotyping assays for hepatitis C virus (Serotyping 1-6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were compared to a standardized genotyping assay (Inno-LiPA HCV II; Innogenetics, Belgium) using serum samples collected from 126 patients chronically infected with hepatitis C virus. Serotyping was positive in 87% and 80% of the samples tested with Murex and Chiron, respectively, and concordant with genotyping in 93% and 88%, respectively. Sequence analysis of the NS5b region of 15 samples with discrepant typing results confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay serotyping method was less sensitive for identifying genotype 2 (P<0.05). The concordance with genotyping of the RIBA serotyping method was lower for genotype 2 than for the other genotypes (P<0.05).
Sandres
K
K
Service de Virologie, Hĵpital PURPAN, Toulouse, France. sandres.k@chu-toulouse.fr
Dubois
M
M
Pasquier
C
C
Puel
J
J
Izopet
J
J
D000914:Antibodies, Viral / Q000032:analysis*; D016009:Chi-Square Distribution; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral / Q000235:genetics*; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics* / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000097:blood; D006801:Humans; D007124:Immunoenzyme Techniques; D011336:Probability; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods
D000914:Antibodies, Viral; D004279:DNA, Viral