Detection of circulating antigens by ELISA using penicillinase in sera of mice infected with Toxoplasma gondii.
An enzyme linked immunosorbent assay (ELISA) has been developed using penicillinase as an enzyme marker for the detection of Toxoplasma gondii antigens during the acute stage of infection in the sera of experimentally infected mice. Circulating antigens were detected as early as 2 days after the infection and all mice had antigenemia by fourth day. The developed ELISA test is sensitive, specific and reproducible.
Bhopale
G M
GM
Laboratory of Parasitology and Immunodiagnostocs, Research & Development Division, Hindustan Antibiotics Limited, Pimpri, Pune-411 018, India.
Naik
S R
SR
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D051379:Mice; D010405:Penicillinase / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis / Q000382:microbiology*
D000953:Antigens, Protozoan; D015415:Biomarkers; D010405:Penicillinase