title: Cutaneous and subcutaneous mast cell and eosinophil responses after challenge in mice vaccinated with living infective third-stage hookworm larvae.
abstract: OBJECTIVE
  To examine the quantitative and qualitative alterations in mast cells and eosinophils distributed in the cutaneous and subcutaneous tissues of hookworm-infected, uninfected and vaccinated mice.
  
  
  METHODS
  Outbred male Kunming strain mice, weighing 18-22 g, were vaccinated thrice by subcutaneous inoculation with 500 living third-stage infective larvae (L3) of Ancylostoma caninum (A. caninum) every 2 weeks, and then challenged subcutaneously with 500 L3 one week after the final immunization. Uninfected mice and non-immunized mice but infected with L3 served as controls. The abdominal skin at the site of percutaneous entry was excised from challenged mice at intervals between 6 hours and 7 days after challenge, fixed, and then examined by light microscopy after staining with either toluidine blue or hematoxylin and eosin.
  
  
  RESULTS
  The total number of mast cells appearing in cutaneous, and subcutaneous tissues, and underlying abdominal musculature of immunized mice increased significantly compared with non-immunized mice. Mast cells from hookworm-infected mice showed evidence of membrane rupture and degranulation in contrast to the intact appearance of mast cells from uninfected mice. The degree of mast cell degranulation was greater in vaccinated and challenged mice when compared with non-immunized and infected mice. Similarly, eosinophilic infiltration was greatly enhanced after L3 infection. Tissues from vaccinated mice had a greater number of eosinophils than non-immunized mice after infection.
  
  
  CONCLUSIONS
  Mast cell alterations appearing earlier than tissue eosinophilic infiltration is major inflammatory response to Ancylostoma caninum (A. caninum) L3 infection in mice. These processes are more obvious in L3-vaccinated mice.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Xiao
authors.forename: H
authors.initials: H
authors.lastname: Ren
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Feng
authors.forename: P J
authors.initials: PJ
authors.lastname: Hotez
mesh_terms: D000722:Ancylostoma / Q000276:immunology*; D000724:Ancylostomiasis / Q000276:immunology*; D000818:Animals; D015550:Cell Degranulation; D004804:Eosinophils / Q000473:pathology; D007814:Larva; D008297:Male; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D012867:Skin / Q000473:pathology*; D014611:Vaccination; D014612:Vaccines / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology
chemical_list: D014612:Vaccines; D015164:Vaccines, Inactivated