Ammocidin, a new apoptosis inducer in Ras-dependent cells from Saccharothrix sp. I. Production, isolation and biological activity.
A new apoptosis inducer, ammocidin, was isolated from the culture broth of Saccharothrix sp. AJ9571. Ammocidin induced apoptotic cell death in Ras-dependent Ba/F3-V12 cells with an IC50 of 66 ng/ml. No cell death was observed in IL-3-dependent Ba/F3-V12 cells at less than 100 microg/ml of ammocidin. Ammocidin significantly reduced the phosphorylation level of MAPK and S6K that mediate the anti-apoptotic function of Ras.
Murakami
R
R
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.
Tomikawa
T
T
Shin-ya
K
K
Shinozaki
J
J
Kajiura
T
T
Kinoshita
T
T
Miyajima
A
A
Seto
H
H
Hayakawa
Y
Y
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D018631:ras Proteins / Q000378:metabolism
D018942:Macrolides; C440627:ammocidin; D018631:ras Proteins