Identification of Mycobacterium avium subsp. paratuberculosis in biopsy specimens from patients with Crohn's disease identified by in situ hybridization.
Crohn's disease is a chronic inflammatory disease of the gastrointestinal tract of unknown etiology. We report on the presence of cell wall-deficient Mycobacterium avium subsp. paratuberculosis in 35 of 48 paraffin-embedded tissue specimens from 33 patients with Crohn's disease by in situ hybridization with IS900 as a probe.
Sechi
L A
LA
Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, 07100 Sassari, Italy. sechila@ssmain.uniss.it
Mura
M
M
Tanda
F
F
Lissia
A
A
Solinas
A
A
Fadda
G
G
Zanetti
S
S
Manuela
M
M
Francesco
T
T
Amelia
L
L
Antonello
S
S
Giovanni
F
F
Stefania
Z
Z
D001706:Biopsy; D003424:Crohn Disease / Q000382:microbiology*; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics*; D004064:Digestive System / Q000382:microbiology*; D006801:Humans; D017403:In Situ Hybridization; D016927:Mycobacterium avium subsp. paratuberculosis / Q000145:classification* / Q000235:genetics
D015342:DNA Probes; D004251:DNA Transposable Elements