Nanoliter capillary electrochromatography columns based on collocated monolithic support structures molded in poly(dimethyl siloxane).
Following current trends in miniaturization of analytical chemistry, an inexpensive disposable analytical tool in the form of a liquid chromatography column fabricated on a poly(dimethyl siloxane) (PDMS) chip was created. Ease of fabricating the chromatography column was demonstrated by molding collocated monolithic support structures (COMOSS) directly in the column. Positive photo-resist, SPR 220, was used to create column structures on a negative relief master providing final channel dimensions of 2.7-5.2 microm wide by 10.0 microm deep, while monolithic dimensions were 9.8 x 9.8 x 10.0 microm - 12.3 x 12.3 x 10.0 microm. The ability to separate biological samples such as peptides from a tryptic digest of fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) was shown. Separations in capillary electrochromatographic (CEC) mode were performed yielding column efficiencies of 4.0 x 10(5) plates/m.
Slentz
B E
BE
Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, USA.
Penner
N A
NA
Lugowska
E
E
Regnier
F
F
D000818:Animals; D002197:Capillary Action; D002417:Cattle; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000302:isolation & purification*; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D012828:Silicones; D013050:Spectrometry, Fluorescence
D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D007202:Indicators and Reagents; D012828:Silicones; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; C013830:baysilon; D016650:Fluorescein-5-isothiocyanate