title: Application of PCR in situ method for detection of human cytomegalovirus (HCMV) DNA.
abstract: Present HCMV diagnosis is relatively slow and inefficient. We applied PCR in situ to 15 samples of human salivary glands, 20 cytospins from blood, and 10 human fibroblast samples in order to detect the presence of HCMV DNA. The results indicate that PCR in situ is an effective and very sensitive method for detection of HCMV infections in variety of specimens.
authors.affiliation: Insitute of Molecular Biology, Jagiellonian University, Kraków, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kosz-Vnenchak
authors.forename: A
authors.initials: A
authors.lastname: Grabiec
authors.forename: B
authors.initials: B
authors.lastname: Zawilińska
mesh_terms: D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D016867:Immunocompromised Host; D016133:Polymerase Chain Reaction / Q000379:methods*; D012469:Salivary Glands / Q000821:virology
chemical_list: D004279:DNA, Viral