title: Induction of immunity using oral DNA vaccines expressing the measles virus nucleocapsid protein.
abstract: In this study, we have examined the feasibility of immunisation against measles with plasmid DNA administered by the oral route. After the oral administration, in two 50 microg doses, of poly(DL-lactide-co-glycolide) (PLGA)-encapsulated DNA expressing measles virus nucleoprotein, increasing titres of N-specific serum IgG antibodies were observed in three of ten C3H/He mice over a period of three months. In comparison, oral vaccination of mice with a replication-defective recombinant adenovirus expressing the same transgene induced serum IgG in all animals tested. We also obtained preliminary indication of adjuvant-like activity of PLGA particles when coadministered intraperitoneally (i.p.) with naked plasmid DNA. These experiments demonstrate that oral delivery of either PLGA-encapsulated plasmid DNA or viral vectored DNA is capable of eliciting strong immune responses in mice. We propose that oral administration of biodegradable microparticles offers a novel strategy for future vaccine design for the safe delivery of DNA to mucosal surfaces.
authors.affiliation: Centre for Applied Microbiology and Research (CAMR), Salisbury, UK.
authors.forename: A R
authors.initials: AR
authors.lastname: Fooks
authors.forename: S A
authors.initials: SA
authors.lastname: Sharpe
authors.forename: J A
authors.initials: JA
authors.lastname: Shallcross
authors.forename: J C
authors.initials: JC
authors.lastname: Clegg
authors.forename: M P
authors.initials: MP
authors.lastname: Cranage
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D002214:Capsules; D002460:Cell Line; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage; D008458:Measles Vaccine / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D011100:Polyglycolic Acid / Q000008:administration & dosage; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D002214:Capsules; D008458:Measles Vaccine; D019590:Nucleocapsid Proteins; D011108:Polymers; D019444:Vaccines, DNA; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid