title: [Immunohistochemical and ultrastructural study of basement membrane in oral verrucous carcinoma].
abstract: OBJECTIVE
  To study the immunohistochemical alteration of basement membrane (BM) type IV collagen and laminin in oral verrucous carcinoma and its BM ultrastructural variations.
  
  
  METHODS
  16 cases of oral verrucous carcinoma (OVC), 10 cases of oral squamous cell carcinoma (OSCC) and 9 cases of oral mild to severe epithelial dysplasia (OMSD) were studied by using a immunohistochemical S-P method, and the results were analyzed by quantitative method. 3 cases of OVC were observed by TEM.
  
  
  RESULTS
  The BM in OVC was thicker than in OSCC and OMSD. TEM found the basal lamina in some areas showed a marked reduplication. The BM in OVC was generally intact (13/16), whereas in OSCC it was mostly discontinuous (9/10), especially around the neoplasm front or the small cord consisted of a few cells, and mostly continuous in OMSD (6/9). There was a stromal inflammatory infiltration around tumor nests for all the oral lesions, but it was much heavier in OVC than that in OSCC and OMSD (P < 0.05). There was a positive correlation between intraepithelial lymphocytic infiltration and the BM continuity for OVC(P < 0.01).
  
  
  CONCLUSIONS
  The more continuous BM and the heavier inflammatory infiltration in the connective tissue of OVC may be related to its biological behavior.
authors.affiliation: Department of Pathology, School of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Chen
mesh_terms: D001485:Basement Membrane / Q000737:chemistry / Q000648:ultrastructure; D018289:Carcinoma, Verrucous / Q000737:chemistry* / Q000648:ultrastructure; D024141:Collagen Type IV / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D024141:Collagen Type IV; D007797:Laminin