title: Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria.
abstract: Three types of nucleoside diphosphate kinases (NDPKs) are found in plants but the intra-cellular compartmentation of these proteins is not certain, especially the location of the recently identified type III proteins. Through the fractionation of plant mitochondria from potato and Arabidopsis, display of protein profiles by 2D gel electrophoresis, and identification by mass spectrometry, we present the first direct evidence that type III proteins are localized in the inter-membrane space of plant mitochondria. The possible metabolic functions of NDPK III are discussed in light of its sub-cellular localization.
authors.affiliation: Department of Plant Sciences, University of Oxford, UK.
authors.forename: L J
authors.initials: LJ
authors.lastname: Sweetlove
authors.forename: B
authors.initials: B
authors.lastname: Mowday
authors.forename: H F
authors.initials: HF
authors.lastname: Hebestreit
authors.forename: C J
authors.initials: CJ
authors.lastname: Leaver
authors.forename: A H
authors.initials: AH
authors.lastname: Millar
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000166:cytology / Q000201:enzymology*
chemical_list: D009701:Nucleoside-Diphosphate Kinase