title: Diversity of marine biotoxins in the near-shore ocean area: presence of a palytoxin-like entity at Barber's Point Harbor, Oahu.
abstract: The presence of palytoxin or palytoxin-like compounds in fish extracts has been presented in this study. The hemolytic assay with sheep erythrocytes demonstrated the occurrence of hemolytic factors in fish extracts of Hawaiian reef fish from Barber's Point, Oahu. The rabbit anti-palytoxin inhibition assay with fish extracts and sheep erythrocytes demonstrated that palytoxin or its congener contributed to the lysis of sheep erythrocytes. From these results, it was concluded that sheep erythrocyte hemolysis was caused by palytoxin or palytoxin-like factors present in the fish extracts. Moderate correlation (R2) between mouse toxicity and sheep erythrocyte hemolysis was shown with 50 microg (R2 = 0.48) and 100 microg (R2 = 0.45) extracts. An inverse correlation of R2 = 0.64 was shown between hemolysis and MIA endpoint.
authors.affiliation: Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Wachi
authors.forename: Y
authors.initials: Y
authors.lastname: Hokama
mesh_terms: D000178:Acrylamides / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D003064:Cnidarian Venoms / Q000032:analysis / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D005399:Fishes; D006254:Hawaii; D006461:Hemolysis / Q000187:drug effects; D006462:Hemolytic Plaque Technique; D007118:Immunoassay; D066298:In Vitro Techniques; D051379:Mice; D009132:Muscles / Q000737:chemistry; D011817:Rabbits; D012756:Sheep; D023382:Toxicity Tests, Acute; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins