Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells.
Surfactant protein (SP) D functions as a soluble pattern recognition molecule to mediate the clearance of pathogens by phagocytes in the innate immune response. We hypothesize that SP-D may also interact with dendritic cells, the most potent antigen presenting cell, to enhance uptake and presentation of bacterial antigens. Using mouse bone marrow-derived dendritic cells, we show that SP-D binds to immature dendritic cells in a dose-, carbohydrate-, and calcium-dependent manner, whereas SP-D binding to mature dendritic cells is reduced. SP-D also binds to Escherichia coli HB101 and enhances its association with dendritic cells. Additionally, SP-D enhances the antigen presentation of an ovalbumin fusion protein expressed in E. coli HB101 to ovalbumin-specific major histocompatibility complex class II T cell hybridomas. The enhancement of antigen presentation by SP-D is dose dependent and is not shared by other collectin-like proteins tested. These studies demonstrate that SP-D augments antigen presentation by dendritic cells and suggest that innate immune molecules such as SP-D may help initiate an adaptive immune response for the purpose of resolving an infection.
Brinker
K G
KG
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Martin
E
E
Borron
P
P
Mostaghel
E
E
Doyle
C
C
Harding
C V
CV
Wright
J R
JR
D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D011485:Protein Binding / Q000276:immunology; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*
D000942:Antigens, Bacterial; D000939:Epitopes; D006023:Glycoproteins; D037663:Pulmonary Surfactant-Associated Protein D; D011663:Pulmonary Surfactants; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010047:Ovalbumin