Effects of liposomes of different phospholipid composition on the induction of respiratory burst in human blood monocytes and alveolar macrophages.
Liposomes made from phosphatidylcholine (PC) or PC-fatty acid ester mixtures have been shown to induce an activation of the respiratory burst in human blood monocytes and alveolar macrophages (AM). Incorporation of 1,2-diacylglycerol or arachidonic acid into PC liposomes significantly enhanced the effect. In the case of AM, the effect of PC liposomes was similar to those of phorbol 12-myristate 13-acetate (PMA) and the ionophore A 23187, while in monocytes, PMA and A 23187 induced a stronger respiratory burst than PC liposomes. In the presence of PMA, higher liposomal concentrations were required to produce the maximum activation of the respiratory burst in both types of cells.
Selishcheva
A A
AA
Faculty of Biology, Lomonosov Moscow State University, Vorobyevy Gory, Russia.
Atruz
O M
OM
Sorokoumova
G M
GM
Skrjabin
G A
GA
Vasilenko
I A
IA
Chuchalin
A G
AG
D016718:Arachidonic Acid / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D006801:Humans; D007476:Ionophores / Q000494:pharmacology; D008081:Liposomes / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D019255:NADPH Oxidases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
C011439:1,2-diacylglycerol; D004075:Diglycerides; D005227:Fatty Acids; D007476:Ionophores; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D016718:Arachidonic Acid; D000001:Calcimycin; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate