Investigation of protein-styrene oxide adducts as a molecular biomarker of human exposed to styrene.
Hemoglobin-styrene oxide adducts in blood have been studied as a molecular biomarker of worker exposed to styrene. Determination of protein-styrene oxide adducts in different biological samples with modified Raney-Ni procedure is described in this paper. The following biological samples have been investigated: fresh rat blood reacted with styrene oxide in vitro; rat blood reacted with styrene or styrene oxide in vivo; vein blood from workers exposed to styrene in two factories. The data showed that there was a good linear dose-response relationship between reacting dose of styrene oxide or styrene and amount of protein-styrene oxide adducts in both in vitro and in vivo experiments. For human samples, a dose-response relationship between protein adducts and styrene exposure can be found in glass fiber factory, but not in piano manufacture plant.
Liu
S F
SF
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. SFliu@mail.rcees.ac.cn
Fang
Q M
QM
Jin
Z L
ZL
Rappaport
M S
MS
D000818:Animals; D015415:Biomarkers; D004305:Dose-Response Relationship, Drug; D005898:Glass; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D007221:Industry; D008420:Manufactured Materials; D016273:Occupational Exposure; D051381:Rats; D020058:Styrene / Q000737:chemistry*
D015415:Biomarkers; D006454:Hemoglobins; D020058:Styrene