title: Mus81-Eme1 are essential components of a Holliday junction resolvase.
abstract: Mus81, a fission yeast protein related to the XPF subunit of ERCC1-XPF nucleotide excision repair endonuclease, is essential for meiosis and important for coping with stalled replication forks. These processes require resolution of X-shaped DNA structures known as Holliday junctions. We report that Mus81 and an associated protein Eme1 are components of an endonuclease that resolves Holliday junctions into linear duplex products. Mus81 and Eme1 are required during meiosis at a late step of meiotic recombination. The mus81 meiotic defect is rescued by expression of a bacterial Holliday junction resolvase. These findings constitute strong evidence that Mus81 and Eme1 are subunits of a nuclear Holliday junction resolvase.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: M N
authors.initials: MN
authors.lastname: Boddy
authors.forename: Pierre-Henri L
authors.initials: PHL
authors.lastname: Gaillard
authors.forename: W H
authors.initials: WH
authors.lastname: McDonald
authors.forename: P
authors.initials: P
authors.lastname: Shanahan
authors.forename: J R
authors.initials: JR
authors.lastname: Yates
authors.forename: P
authors.initials: P
authors.lastname: Russell
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology; D004271:DNA, Fungal / Q000235:genetics / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004720:Endonucleases / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology; D045565:Holliday Junction Resolvases; D046911:Macromolecular Substances; D008540:Meiosis / Q000502:physiology; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000502:physiology; D011995:Recombination, Genetic / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014162:Transfection
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C442837:Eme1 protein, S pombe; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C411624:MUS81 protein, S cerevisiae; D045565:Holliday Junction Resolvases; C112057:RusA protein, E coli