Miniaturization of capillary isoelectric focusing.
Miniaturization of whole-column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two pI markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light-emitting diode (LED) was used as the light source for the whole-column absorbance imaging detection. The focusing of both the pI markers and myoglobin were observed with the LED light source. The whole-column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.
Wu
X Z
XZ
Department of Chemistry, University of Waterloo, ON, Canada.
Sze
S K
SK
Pawliszyn
J
J
D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D007525:Isoelectric Focusing / Q000295:instrumentation*; D007526:Isoelectric Point; D008027:Light; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D009211:Myoglobin / Q000737:chemistry; D020033:Protein Isoforms / Q000737:chemistry
D009211:Myoglobin; D020033:Protein Isoforms