[Cloning and sequencing of the VL and VH genes from the anti-vascular endothelial growth factor McAb hybridoma cell line].
OBJECTIVE
The light chain variable region (VL) and heavy chain variable region (VH) genes from the anti-VEGF165 McAb hybridoma cell line were cloned for construction of it's chimeric antibody.


METHODS
The VL and VH genes were amplified from the total RNA of the VmD11 hybridoma with RT-PCR and cloned into vectors, and then analyzed by sequencing.


RESULTS
The 320 bp VL gene belong to mouse kappa light chain subgroup V, the 354 bp VH gene belong to mouse heavy chains subgroup I (B).


CONCLUSIONS
The nucleotide sequence analysis indicated that the cloned genes coded the light and heavy chain variable region of mouse respectively.
Ran
Y
Y
Cancer Institute, CAMS and PUMC, Beijing 100021.
Yang
Z
Z
Wang
G
G
Dong
Z
Z
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D016228:Endothelial Growth Factors / Q000276:immunology*; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008222:Lymphokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors