Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro.
Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.
Kamau
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SW
Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland.
Grimm
F
F
Hehl
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AB
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D015415:Biomarkers; D003513:Cycloheximide / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D018314:Leishmania infantum / Q000187:drug effects* / Q000254:growth & development; D008164:Luminescent Proteins / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
D000981:Antiprotozoal Agents; D015415:Biomarkers; D008164:Luminescent Proteins; D011500:Protein Synthesis Inhibitors; D049452:Green Fluorescent Proteins; D011691:Puromycin; D000493:Allopurinol; D003513:Cycloheximide