title: Grafting neural stem cells improved the impaired spatial recognition in ischemic rats.
abstract: To determine the possible therapeutic potential of neural stem cells (NSCs) introduced into the damaged central nervous system, we grafted adult hippocampus-derived NSCs into the hippocampus of rats with transient global ischemia. Transient four-vessel occlusion yielded 90-95% losses of pyramidal neurons in the hippocampal CA1 region. In this region, 1-3% of the grafted cells survived; and 3-9% of them expressed NeuN, a neuronal marker. Rats with more than 120 NeuN-positive cells showed partial improvement of impaired spatial learning in a water maze test. These results suggest that NSCs grafted in the ischemic brain are able to differentiate into neurons and to improve spatial recognition.
authors.affiliation: Department of Neurosurgery, Clinical Neuroscience, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Toda
authors.forename: J
authors.initials: J
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Iwakami
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Hoki
authors.forename: K
authors.initials: K
authors.lastname: Mozumi-Kitamura
authors.forename: S
authors.initials: S
authors.lastname: Ono
authors.forename: N
authors.initials: N
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods; D006624:Hippocampus / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D011916:Rats, Inbred F344; D021641:Recognition, Psychology / Q000502:physiology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology