RAGE expression and AGE-induced MAP kinase activation in Caco-2 cells.
RAGE (receptor for advanced glycation end products) is a multiligand cell surface molecule of the immunoglobulin superfamily. It was originally described as a receptor for protein adducts formed by glycoxidation (AGEs) that accumulate in diseases such as diabetes and renal failure. Performing RT-PCR and Western blot analysis we intended to determine RAGE expression in the human colon adenocarcinoma cell line Caco-2. Moreover, Caco-2 cells were incubated in the presence of AGEs. Since RAGE ligation triggers the p21(ras) signal transduction pathway the activation state of p44/42 (ERK1/2) MAP kinases was determined. Here we demonstrate for the first time that Caco-2 cells express RAGE and that administration of the food-derived casein-linked AGE N(epsilon)-(carboxymethyl)lysine (Cas-CML) results in Caco-2 p44/42 (ERK1/2) MAP kinase activation.
Zill
H
H
Institute of Human Nutrition and Food Science, University of Kiel, Duesternbrooker Weg 17, D-24105 Kiel, Germany. hzill@nutrfoodsc.uni-kiel.de
Günther
R
R
Erbersdobler
H F
HF
Fölsch
U R
UR
Faist
V
V
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D018938:Caco-2 Cells; D002364:Caseins / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011489:Protein Denaturation; D012333:RNA, Messenger / Q000096:biosynthesis; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D002364:Caseins; D017127:Glycation End Products, Advanced; D011480:Protease Inhibitors; D012333:RNA, Messenger; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; C048496:N(6)-carboxymethyllysine; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D006026:Glycoside Hydrolases; D008239:Lysine