Detection of Listeria monocytogenes by polymerase chain reaction oriented to inlB gene.
Primers were designed for the detection of Listeria monocytogenes by the polymerase chain reaction oriented to specific sequences of the inlB gene encoding an internalin. At optimized reaction conditions, 100% sensitivity (on a panel of 33 strains of L. monocytogenes) and 100% specificity (on panels of 15 strains of other Listeria spp. and 41 other bacteria), were determined for the inlB-L/R primers. The detection limit of PCR with these primers was 10(4) cfu/ml and was not affected by up to 10(8) cfu/ml of L. innocua.
Pangallo
D
D
Department of Microbiology and Chemistry--Food Research Institute, Bratislava, Slovakia.
Kaclíková
E
E
Kuchta
T
T
Drahovská
H
H
D001426:Bacterial Proteins; D017931:DNA Primers; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D017931:DNA Primers; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes