[Study on the effect of human herpesvirus 6 on replication of Epstein-Barr virus].
OBJECTIVE
To examine the effect of human herpesvirus 6(HHV-6) on the replication of Epstein-Barr virus (EBV) in cell lines.


METHODS
Both EBV-infected Raji cells and EBV-transformed lymphoblastoid cell lines (LCL) were infected with HHV-6. Immunofluoresence assay (IFA) with monoclonal antibodies (MAb) against HHV-6 was applied to confirm the infection of HHV-6 in the two cell lines. Expression of EBV antigen was examined by IFA using human anti-EBV serum.


RESULTS
Following HHV-6 infection, cytopathic effects (CPE) were observed in Raji cells but not in LCL. HHV-6 were detected in both cell lines by IFA with anti-HHV-6 MAb, but not in controls. EBV antigens were detected by IFA with human serum against EBV in both HHV-6 infected cell lines.


CONCLUSIONS
Data in this study suggest that HHV-6 promotes the expression of EBV antigen and may contribute to the pathogenesis of nasopharyngeal carcinoma in cooperation with EBV.
Xia
Q
Q
Qingdao Municipal Hospital, Qingdao 266011.
Meng
G
G
Ai
Y
Y
D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000473:pathology / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000502:physiology*; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D009303:Nasopharyngeal Neoplasms / Q000821:virology; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D000956:Antigens, Viral