Strategies for signal amplification in nucleic acid detection.
Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction, Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification) are summarized in the present review, together with their advantages and limitations.
Andras
S C
SC
Babes-Boylai University, Faculty of Biology and Geology, Ecology and Genetics Department, Str. Clinicilor 5-7, Cluj-Napoca 3400, Romania.
Power
J B
JB
Cocking
E C
EC
Davey
M R
MR
D015342:DNA Probes; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009696:Nucleic Acids / Q000032:analysis*
D015342:DNA Probes; D009696:Nucleic Acids