title: The immunohistochemical detection of Mycoplasma bovis and bovine viral diarrhea virus in tissues of feedlot cattle with chronic, unresponsive respiratory disease and/or arthritis.
abstract: The purpose of this study was to determine the frequency of selected pathogens in the tissues of a group of feedlot cattle with chronic disease (most often respiratory disease and/or arthritis). Samples of lung and joint tissues from 49 feedlot animals that had failed to respond to antibiotic therapy were tested by immunohistochemical staining for the antigens of Mycoplasma bovis, Haemophilus somnus, Pasteurella (Mannheimia) hemolytica, and bovine viral diarrhea virus (BVDV). Mycoplasma bovis was demonstrated in over 80% of cases, including in 45% of joints and 71% of lungs tested. Mycoplasma bovis was the only bacterial pathogen identified in the joints. Haemophilus somnus and Pasteurella (Mannheimia) haemolytica were found in 14% and 23% of cases, respectively, and were confined to the lungs in all instances. Infection with BVDV was demonstrated in over 40% of cases. Mycoplasma bovis and BVDV were the most common pathogens persisting in the tissues of these animals that had failed to respond to antibiotic therapy.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4.
authors.forename: D M
authors.initials: DM
authors.lastname: Haines
authors.forename: K M
authors.initials: KM
authors.lastname: Martin
authors.forename: E G
authors.initials: EG
authors.lastname: Clark
authors.forename: G K
authors.initials: GK
authors.lastname: Jim
authors.forename: E D
authors.initials: ED
authors.lastname: Janzen
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001168:Arthritis / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000209:etiology; D002908:Chronic Disease; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry / Q000379:methods / Q000662:veterinary; D008168:Lung / Q000382:microbiology / Q000473:pathology / Q000821:virology; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000209:etiology / Q000662:veterinary*; D013583:Synovial Membrane / Q000382:microbiology / Q000473:pathology / Q000821:virology
chemical_list: D000942:Antigens, Bacterial; D000956:Antigens, Viral