title: Refraction imaging and histologic correlation in excised tissue from a normal human lung: preliminary report.
abstract: OBJECTIVE
  The authors performed this study to evaluate the spatial resolution of refraction imaging performed with synchrotron radiation (SR) on 2-mm-thick sections of excised normal human lung.
  
  
  METHODS
  Refraction images of specimens from a normal human lung were obtained with 19 keV, a 5-second exposure time, and a 2.4 x 2.4-cm field of view. The specimen-detector distance was 83 cm.
  
  
  RESULTS
  Structures of secondary pulmonary lobule consisting of pulmonary arteriole, terminal bronchiole, air sac, and venules in the interlobular septum in normal lung were clearly illustrated on refraction images obtained with SR. The imaging findings showed good correlation with those from histologic examination. The spatial resolution of the refraction images was approximately 10-30 microm, as measured with light microscopic analysis of specimens stained with hematoxylin-eosin.
  
  
  CONCLUSIONS
  With refinement, this technique may have some practical purpose for diagnosing lung disease in vivo.
authors.affiliation: Department of Radiology, Hyogo Medical Center for Adults, Akashi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kono
authors.forename: C
authors.initials: C
authors.lastname: Ohbayashi
authors.forename: K
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohno
authors.forename: S
authors.initials: S
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Sugimura
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000368:Aged; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D008168:Lung / Q000033:anatomy & histology / Q000000981:diagnostic imaging*; D008171:Lung Diseases / Q000000981:diagnostic imaging; D008297:Male; D008954:Models, Biological; D011859:Radiography; D017356:Synchrotrons