High-capacity 'gutless' adenoviral vectors.
Adenoviral vectors are promising gene transfer vehicles for different gene therapy applications. High-capacity adenoviral (HC-Ad) vectors address some of the problems that have been observed with replication-defective, E1-deleted first-generation adenoviral vectors: toxicity and immunogenicity due to viral gene expression and 7 to 8 kb capacity limit for the transport of therapeutic DNA. This review summarizes HC-Ad vector-related publications from the past 18 months that are mainly concerned with vector design/production and in vivo applications in different murine models.
Kochanek
S
S
Center for Molecular Medicine, University of Cologne, Germany. Stefan.Kochanek@medizin.uni-koeln.de
Schiedner
G
G
Volpers
C
C
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002213:Capsid / Q000235:genetics; D002490:Central Nervous System / Q000378:metabolism; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods* / Q000639:trends; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D016662:Virus Integration / Q000235:genetics
D004274:DNA, Recombinant