Molecular characterization of Hop mosaic virus: its serological and molecular relationships to Hop latent virus.
The 3'-terminal sequence of hop mosaic virus (HpMV) genomic RNA was determined. A cDNA of approximately 1.8 kbp was amplified from the HpMV genome by 3' RACE using a degenerate primer, which was designed to anneal to the overlapping region of open reading frames (ORFs) 2 and 3 of eight carlavirus genomes. The sequence contained three ORFs, encoding proteins of 7-, 34-, and 11-kDa, which corresponded to ORFs 4, 5, and 6 of the carlavirus genome, respectively. The amino acid sequence of ORF 5, encoding the coat protein (CP) of HpMV, shows the highest identity (67%) to that of Hop latent virus (HpLV). The HpMV CP N-terminal sequence differs from that of HpLV, but the central and C-terminal sequences of the CP of both viruses are similar. The sequence similarity possibly causes the cross-reaction of heterologous antibodies of HpMV and HpLV. Phylogenetic analyses based on the CP amino acid and 3' non-coding region sequences indicate close relationships among HpMV, HpLV, and Potato virus M. We report here the first molecular characterization of HpMV genomic RNA.
Hataya
T
T
Laboratory of Pathogen-Plant Interactions, Plant Breeding Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. hataya@res.agr.hokudai.ac.jp
Arimoto
R
R
Suda
N
N
Uyeda
I
I
D020413:3' Untranslated Regions / Q000737:chemistry; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D017788:Carlavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D018076:DNA, Complementary / Q000737:chemistry; D027582:Humulus / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny
D020413:3' Untranslated Regions; D000914:Antibodies, Viral; D018076:DNA, Complementary