title: Gating kinetics of the alpha1I T-type calcium channel.
abstract: The alpha1I T-type calcium channel inactivates almost 10-fold more slowly than the other family members (alpha1G and alpha1H) or most native T-channels. We have examined the underlying mechanisms using whole-cell recordings from rat alpha1I stably expressed in HEK293 cells. We found several kinetic differences between alpha1G and alpha1I, including some properties that at first appear qualitatively different. Notably, alpha1I tail currents require two or even three exponentials, whereas alpha1G tails were well described by a single exponential over a wide voltage range. Also, closed-state inactivation is more significant for alpha1I, even for relatively strong depolarizations. Despite these differences, gating of alpha1I can be described by the same kinetic scheme used for alpha1G, where voltage sensor movement is allosterically coupled to inactivation. Nearly all of the rate constants in the model are 5-12-fold slower for alpha1I, but the microscopic rate for channel closing is fourfold faster. This suggests that T-channels share a common gating mechanism, but with considerable quantitative variability.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Frazier
authors.forename: J R
authors.initials: JR
authors.lastname: Serrano
authors.forename: E G
authors.initials: EG
authors.lastname: George
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: A
authors.initials: A
authors.lastname: Viswanathan
authors.forename: E
authors.initials: E
authors.lastname: Perez-Reyes
authors.forename: S W
authors.initials: SW
authors.lastname: Jones
mesh_terms: D000200:Action Potentials; D000818:Animals; D020747:Calcium Channels, T-Type / Q000502:physiology*; D018929:Cell Culture Techniques; D004594:Electrophysiology; D015640:Ion Channel Gating / Q000502:physiology*; D007700:Kinetics; D008962:Models, Theoretical; D051381:Rats
chemical_list: D020747:Calcium Channels, T-Type