title: Production of intracellular milk-clotting enzyme in submerged cultures of Fusarium subglutinans.
abstract: Fusarium subglutinans (Wollenweber and Reinking) Nelson et al. was found to produce intracellular milk-clotting enzyme (MCE) with good milk-clotting activity (MCA). The crude activity of the produced enzyme was recorded as optimum at 55 degrees C and pH 4.5. The highest yield i.e. 78.43 SU/mg dry biomass was obtained after 4 days of rotary shaking at 30 degrees C when the fermentation medium containes wheat flour 2%, glucose 1% and (NH4)2SO4 0.1% with an initial pH value 6.0. Under these conditions, the maximum ratio of MCA to proteolytic activity (PA) amounting to 603.31 SU/PU mg(-1) was also achieved. Production of intracellular MCE by F. subglutinans was assumed to be active growth-associated type. This enzyme preparation was less active than the calf rennet, but was superior to those of Meito's and Pfizer's rennets.
authors.affiliation: Biology Department, Faculty of Education, Ain Shams Univ., Roxy 11757, Cairo, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: Ghareib
authors.forename: H S
authors.initials: HS
authors.lastname: Hamd
authors.forename: A A
authors.initials: AA
authors.lastname: Khalil
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D002611:Cheese / Q000382:microbiology; D005285:Fermentation; D005670:Fusarium / Q000201:enzymology*; D008892:Milk / Q000737:chemistry; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D010447:Peptide Hydrolases; D016282:Aspartic Acid Endopeptidases; C022517:rennin-like enzyme (Aspergillus ochraceus)