Grafting neural stem cells improved the impaired spatial recognition in ischemic rats.
To determine the possible therapeutic potential of neural stem cells (NSCs) introduced into the damaged central nervous system, we grafted adult hippocampus-derived NSCs into the hippocampus of rats with transient global ischemia. Transient four-vessel occlusion yielded 90-95% losses of pyramidal neurons in the hippocampal CA1 region. In this region, 1-3% of the grafted cells survived; and 3-9% of them expressed NeuN, a neuronal marker. Rats with more than 120 NeuN-positive cells showed partial improvement of impaired spatial learning in a water maze test. These results suggest that NSCs grafted in the ischemic brain are able to differentiate into neurons and to improve spatial recognition.
Toda
H
H
Department of Neurosurgery, Clinical Neuroscience, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto, 606-8507, Japan.
Takahashi
J
J
Iwakami
N
N
Kimura
T
T
Hoki
S
S
Mozumi-Kitamura
K
K
Ono
S
S
Hashimoto
N
N
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods; D006624:Hippocampus / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D008297:Male; D018782:Maze Learning / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D011916:Rats, Inbred F344; D021641:Recognition, Psychology / Q000502:physiology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology