[Adsorption of TNF alpha onto the amino acid-modified NK-110 resin].
It is a effective way to remove Tumor Necrosis Factor(TNF alpha) from plasma by adsorbent. In the present study, NK-110 was modified by 8 amino acids to prepare the adsorbents to be used in the static adsorption experiments of TNF alpha. We have studied the adsorption capacity, kinetic profiles and adsorption isoterm of Cys modified NK-110, and some comparison were made between Cys modified NK-110 and unmodified one. The experimental results show that the Cys modified NK-110 exhibited superior adsorption capacity which is 7683.80 u/mL, and the adsorption percentage is 85.38% at 120 min in stable adsorption. Compared with unmodified NK-110, the Cys-modified one with high adsorption velocity. Furthermore, adsorption isotherms were also studied on Cys-modified and bare NK-110, bot showed to be of "L" shape at 37 degrees C. The adsorption amount increased as the concentration of TNF alpha increased, however, the adsorption percentage is stable adsorbed by Cys-modified NK-110, whereas it is decreased by bare one. The results demonstrating that Cys can significantly raised the adsorption capacity.
Wei
J
J
Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
Yu
Y T
YT
Kong
D L
DL
D000327:Adsorption; D000596:Amino Acids; D003545:Cysteine; D012117:Resins, Synthetic / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000302:isolation & purification*
D000596:Amino Acids; D012117:Resins, Synthetic; D014409:Tumor Necrosis Factor-alpha; D003545:Cysteine