The role of PDR13 in tolerance to high copper stress in budding yeast.
PDR13 in Saccharomyces cerevisiae contributes to drug resistance via sequential activation of PDR1 and PDR5. In this study, we found that a PDR13 deletion mutant was hypersensitive to Cu(2+) compared to the wild-type counterpart. The Cu(2+) tolerance mechanism mediated by Pdr13 does not seem to involve Pdr1 or Pdr5, since mutants harboring a deletion of either the PDR1 or PDR5 gene did not show elevated Cu(2+) sensitivity. Instead, we found that the PDR13 null mutant could not express CUP1 or CRS5 metallothionein at wild-type levels when subjected to high Cu(2+) stress. These results suggest that Pdr13 contributes to high Cu(2+) tolerance of S. cerevisiae, at least in part, via a mechanism involving metallothionein expression.
Kim
D Y
DY
Division of Molecular Life Sciences, Pohang University of Science and Technology, Pohang 790-784, South Korea.
Song
W Y
WY
Yang
Y Y
YY
Lee
Y
Y
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism; D002352:Carrier Proteins; D003300:Copper / Q000378:metabolism / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D005800:Genes, Fungal; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors
D018528:ATP-Binding Cassette Transporters; C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C085480:PDR1 protein, S cerevisiae; C092438:PDR5 protein, S cerevisiae; C424445:SSZ1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C007121:copper thionein; D002104:Cadmium; D003300:Copper; D008668:Metallothionein