Examination of lactococcal bacteriophage c2 DNA replication using two-dimensional agarose gel electrophoresis.
The ori locus of the prolate-headed lactococcal bacteriophage c2 supports plasmid replication in Lactococcus lactis in the absence of phage infection. To determine whether phage c2 DNA replication is initiated at the ori locus in vivo and to investigate the mechanism of phage DNA replication, replicating intermediates of phage c2 were analyzed using neutral/neutral two-dimensional agarose gel electrophoresis (2D). The 2D data revealed that c2 replicates via a theta mechanism and localized the initiation of theta replication to the ori region of the c2 genome.
Callanan
M J
MJ
Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand. mjcallan@unity.ncsu.edu
O'Toole
P W
PW
Lubbers
M W
MW
Polzin
K M
KM
D001435:Bacteriophages / Q000235:genetics*; D001482:Base Composition; D015139:Blotting, Southern; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D016679:Genome, Viral; D013294:Lactococcus lactis / Q000821:virology*; D018741:Replication Origin / Q000235:genetics
D004279:DNA, Viral