Evidence for evolution of canine parvovirus type 2 in Italy.
Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay.
Buonavoglia
Canio
C
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Martella
Vito
V
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Pratelli
Annamaria
A
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Tempesta
Maria
M
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Cavalli
Alessandra
A
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Buonavoglia
Domenico
D
Department of Pathology and Infectious Diseases, University of Messina, Messina, Italy2.
Bozzo
Giancarlo
G
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Elia
Gabriella
G
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Decaro
Nicola
N
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
Carmichael
Leland
L
James Baker Institute, Cornell University, Ithaca, NY, USA3.
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002213:Capsid / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D003967:Diarrhea / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics* / Q000276:immunology; D004285:Dogs; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D019143:Evolution, Molecular; D014644:Genetic Variation; D007558:Italy; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D020539:Sequence Analysis, Protein
D000956:Antigens, Viral; D017931:DNA Primers; D000939:Epitopes