title: Molecular characterization of first human Bartonella strain isolated in Italy.
abstract: The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain.
authors.affiliation: Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ciervo
authors.forename: A
authors.initials: A
authors.lastname: Petrucca
authors.forename: S
authors.initials: S
authors.lastname: Ciarrocchi
authors.forename: A
authors.initials: A
authors.lastname: Pinto
authors.forename: L
authors.initials: L
authors.lastname: Bonazzi
authors.forename: A
authors.initials: A
authors.lastname: Fabio
authors.forename: E
authors.initials: E
authors.lastname: Farnetti
authors.forename: B B
authors.initials: BB
authors.lastname: Chomel
authors.forename: L
authors.initials: L
authors.lastname: Ciceroni
mesh_terms: D000328:Adult; D016917:Angiomatosis, Bacillary / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000379:methods; D018413:Bartonella quintana / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005227:Fatty Acids / Q000032:analysis; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007558:Italy; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S