title: [cDNA cloning and sequence analysis of human lactoferrin].
abstract: Human lactoferrin (hLF) cDNA was amplified by RT-PCR from normal human mammary tissue obtained from Daxing County of Beijing City of China, and then subcloned into pGEM-T vector. hLF cDNA sequence was determined, which consists of 2136 bp. Comparison with five other hLF cDNA sequences registered in GenBank shows 99% homology in DNA sequence. However, there are two base substitutions (nucleotide 1740 G-->C, nucleotide 1756 T-->C), one of which subsequently leads to an amino acid change (residue 580 Glu-->Asp).
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: H
authors.initials: H
authors.lastname: Cheng
authors.forename: X Z
authors.initials: XZ
authors.lastname: Chen
authors.forename: L D
authors.initials: LD
authors.lastname: Huan
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary; D007781:Lactoferrin