Effect of Astragalin on matrix secretion and beta 1 integrin mRNA expression in human mesangial cells.
OBJECTIVE
To investigate the effect of Astragalin on human renal mesangial cells.


METHODS
Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concentrations in the presence or absence of PDGF-BB, the proliferation and type IV collagen secretion of mesangial cells were measured by MTT assay and ELISA, and expression of beta 1 integrin gene was estimated by reverse transcription-polymerase chain reaction (RT-PCR) method, suspectively.


RESULTS
After 72 hours Astragalin or Astragalin serum treatment, the proliferation of mesangial cells induced by PDGF-BB was inhibited significantly in a dose-dependent manner compared with untreated controls (P < 0.05 and P < 0.01). After 24 hours of Astragalin or Astragalin serum treatment, the secretion of type IV collagen protein in presence of PDGF-BB was significantly decreased and beta 1 integrin mRNA level decreased significantly compared with untreated control (P < 0.05, P < 0.01).


CONCLUSIONS
Astragalin inhibits cell proliferation and matrix over-synthesis which might be mediated, at least, partly by decrease of beta 1 integrin gene over-expression. The study suggested that Astragalin might play a role in preventing the progression of chronic renal diseases.
Ni
Z
Z
Renal Division, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Zhang
Q
Q
Qian
J
J
Wang
L
L
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D015536:Down-Regulation; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology*; D006801:Humans; D019012:Integrin beta1 / Q000096:biosynthesis* / Q000235:genetics; D044949:Kaempferols; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D019012:Integrin beta1; D044949:Kaempferols; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D003094:Collagen; C001579:astragalin