Expression of leukocyte adhesion molecules CD11b, L-selectin and CD45 during hemodialysis.
OBJECTIVE
To examine the gene and protein expression of CD11b, L-selectin and CD45, and the relationship between their expression and leukocytopenia during a hemodialysis session.


METHODS
Ten maintenance hemodialysis patients, 20 uremic non-dialysis patients and 10 healthy volunteers were included in this study. The mRNA expression of CD11b, L-selectin and CD45 in peripheral blood mononuclear cells was detected by RT-PCR, while cell surface expression of these molecules on monocytes was detected by flow cytometry and leukocyte number was counted manually.


RESULTS
After the start of dialysis, both mRNA expression and cell surface expression of CD11b increased rapidly, while those of L-selectin and leukocyte number fell. The mRNA expression of CD45 first decreased and the cell surface expression increased, followed by a return to pre-dialysis levels, by the end of dialysis.


CONCLUSIONS
Hemodialysis using a cuprophane membrane can induce rapid upregulation of CD11b and down-regulation of L-selectin, which might influence the normal adhesion and anti-inflammatory response of leukocytes. In this process CD45 may play a role in regulating and/or transducing activation signal.
Xu
X
X
Renal Division, Renji Hospital, Faculty of Biophysics and Faculty of Biochemistry and Molecular Biology, Shanghai Second Medical University, Shanghai 200001, China.
Yu
Z
Z
Qian
J
J
Shi
X
X
Chen
S
S
D000328:Adult; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D019041:L-Selectin / Q000096:biosynthesis* / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000096:biosynthesis* / Q000235:genetics; D007970:Leukopenia / Q000209:etiology / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D006435:Renal Dialysis
D016177:Macrophage-1 Antigen; D012333:RNA, Messenger; D019041:L-Selectin; D017493:Leukocyte Common Antigens