Binding of SLE autoantibodies to native poly(I), ROS-poly(I) and native DNA: a comparative study.
Reactive oxygen species (ROS) are implicated in a variety of human diseases. The formation of pathogenic anti-DNA antibodies in systemic lupus erythematosus (SLE) has been extensively investigated. ROS-modified DNA has been found to be a better antigen for anti-DNA antibodies found in SLE sera. A comparative binding of SLE autoantibodies with native poly(I), ROS-poly(I) and nDNA has been studied. Affinity-purified SLE IgG exhibited a high degree of specificity towards the ROS-modified poly(I) in comparison to native DNA and native poly(I), reiterated visually by gel retardation assay. The data suggested that hydroxyl radical-modified nucleic acids like RNA and DNA might be agent for the induction of circulating SLE anti-DNA autoantibodies.
Tasneem
S
S
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh 202002, India.
Ali
R
R
D000974:Antibodies, Antinuclear / Q000097:blood / Q000378:metabolism*; D001323:Autoantibodies / Q000378:metabolism*; D001666:Binding Sites, Antibody; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011069:Poly I / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000276:immunology* / Q000378:metabolism
D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D007074:Immunoglobulin G; D017382:Reactive Oxygen Species; D011069:Poly I