The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network.
In order to identify regulators of the Schizosaccharomyces pombe septation initiation network (SIN), which signals the onset of cell division, we have isolated extragenic suppressors of mutations in the GTPase spg1p, which is a central element in this pathway. One of these encodes the protein phosphatase 2A (PP2A) B'-regulatory subunit par1p. Loss of par1p function rescues mutants in cdc11, cdc7, and spg1, but no other SIN mutants. Our data suggest that PP2A-par1p acts as a negative regulator of SIN signalling.
Le Goff
X
X
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges, Switzerland.
Buvelot
S
S
Salimova
E
E
Guerry
F
F
Schmidt
S
S
Cueille
N
N
Cano
E
E
Simanis
V
V
D002455:Cell Division / Q000502:physiology*; D003001:Cloning, Molecular; D005456:Fluorescent Dyes / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins; D054648:Protein Phosphatase 2; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction / Q000502:physiology; D013696:Temperature
D005456:Fluorescent Dyes; D005656:Fungal Proteins; D008164:Luminescent Proteins; C410165:PAR1 protein, S pombe; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2