Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization.
Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80-90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets.
Stassar
M J
MJ
Department of Tumor Progression and Immune Defense, German Cancer Research Center, Heidelberg, 69120.
Devitt
G
G
Brosius
M
M
Rinnab
L
L
Prang
J
J
Schradin
T
T
Simon
J
J
Petersen
S
S
Kopp-Schneider
A
A
Zöller
M
M
D014408:Biomarkers, Tumor / Q000032:analysis*; D015152:Blotting, Northern; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002448:Cell Adhesion; D002470:Cell Survival; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009389:Neovascularization, Pathologic; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D017931:DNA Primers; D004273:DNA, Neoplasm