Collagen II containing a Cys substitution for Arg-alpha1-519: abnormal interactions of the mutated molecules with collagen IX.
Single amino acid substitutions in collagen II cause heterogeneous cartilage disorders including some chondrodysplasias and certain forms of heritable osteoarthritis. In this study, we examined molecular interactions between normal collagen II and collagen IX, and the effect of a Cys substitution for Arg-alpha1-519 in collagen II on these interactions. Binding assays showed that the association equilibrium constant of collagen IX-collagen II interaction is 15 x 10(6) M(-1). Specificity of the interaction was analyzed by the binding of collagen IX to recombinant collagen II variants lacking fragments of 234 amino acids corresponding to particular D-periods. The results indicated that the C-terminal half of collagen II, which includes the D3 and D4 periods, has a high affinity for collagen IX, and that the nontriple helical telopeptides of collagen II are not essential for the specific binding of collagen IX. Computer analysis of the surface of the mutated collagen II and binding assays showed that a Cys substitution for Arg-alpha1-519 changes electrostatic properties around the mutation site, increases the affinity of mutant collagen II for collagen IX, and possibly alters the specificity of the interaction. Thus, the results indicate that interactions between collagen II and collagen IX are site specific and that single amino acid substitutions in collagen II may change the molecular interactions with collagen IX that could destabilize the cartilaginous matrix.
Fertala
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A
Department of Pathology and Molecular Medicine, School of Medicine, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA. Andrzej.Fertala@mail.tju.edu
Sieron
A L
AL
Adachi
E
E
Jimenez
S A
SA
D019943:Amino Acid Substitution; D001120:Arginine / Q000235:genetics*; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D024043:Collagen Type II / Q000737:chemistry* / Q000235:genetics; D024081:Collagen Type IX / Q000737:chemistry* / Q000235:genetics; D003545:Cysteine / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008666:Metalloendopeptidases / Q000378:metabolism; D009154:Mutation; D011347:Procollagen / Q000737:chemistry; D011348:Procollagen N-Endopeptidase / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship
D019485:Bone Morphogenetic Proteins; D024043:Collagen Type II; D024081:Collagen Type IX; D011347:Procollagen; D011994:Recombinant Proteins; D001120:Arginine; D008666:Metalloendopeptidases; D011348:Procollagen N-Endopeptidase; C525705:BMP1 protein, human; D055395:Bone Morphogenetic Protein 1; D003545:Cysteine