Lembehyne A, a spongean polyacetylene, induces neuronal differentiation in neuroblastoma cell.
Lembehyne A (LB-A), a spongean polyacetylene, induced neuronal cell differentiation in a neuroblastoma cell line, Neuro 2A. The LB-A treatment of Neuro 2A cells predominantly resulted in a morphological change with bipolar neurites. The acetylcholinesterase activity of Neuro 2A was also increased by the treatment of LB-A. Furthermore, the cell cycle of Neuro 2A cells was found to be specifically blocked at the G1 phase by LB-A. The structure-activity relationship study using the LB-A analogues revealed the importance of the terminal 1-yn-3-ol and unsaturated long-chain alkyl moieties for the neuronal differentiation activity of LB-A.
Aoki
S
S
Graduate School of Pharmaceutical Sciences, Osaka University, Yamada-oka 1-6, Suita, Osaka 565-0871, Japan.
Matsui
K
K
Takata
T
T
Hong
W
W
Kobayashi
M
M
D000110:Acetylcholinesterase / Q000378:metabolism; D000114:Acetylene / Q000031:analogs & derivatives* / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005233:Fatty Alcohols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D008956:Models, Chemical; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes; D011161:Porifera; D051381:Rats; D013329:Structure-Activity Relationship; D013997:Time Factors
D000480:Alkynes; D005233:Fatty Alcohols; D011108:Polymers; C444641:lembehyne A; D053279:Polyynes; D000110:Acetylcholinesterase; D000114:Acetylene