title: [Efficacy of direct paraffin embedding in cytological analysis of vitreous from proliferative vitreo-retinopathy (PVR)].
abstract: OBJECTIVE
  To assess the efficacy of direct paraffin embedding in cytological analysis of vitreous samples in patients suffering from PVR, against the commonly used method for this purpose (cytospin).
  
  
  METHODS
  Undiluted vitreous or vitreous fluids from 40 subsequent patients with PVR were collected and processed (samples collected from cassette at the end of aspiration line in final stage of vitrectomy process). 40 samples underwent cytospinning, 24 samples were paraffin embedded. Specimens were assessed by an expert ocular pathologist and tested samples were assigned to scoring and non scoring groups (where scoring means at least three cells per field in x60 magnification).
  
  
  RESULTS
  All 24 paraffin embedding samples scored according to criteria while 36 of 40 cytospinned samples complied with scoring criteria. Differences were not statistically significant. Macrophages (87.5%) and pigmented cells (85%) were the most frequent findings.
  
  
  CONCLUSIONS
  Direct paraffin embedding is useful for cytological studies in vitreous samples of PVR patients.
authors.affiliation: Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, España.
authors.forename: E
authors.initials: E
authors.lastname: Rodríguez De La Rúa
authors.forename: F
authors.initials: F
authors.lastname: Martín
authors.forename: M A
authors.initials: MA
authors.lastname: Saornil
authors.forename: N
authors.initials: N
authors.lastname: Fernández
authors.forename: J C
authors.initials: JC
authors.lastname: Pastor
mesh_terms: D006801:Humans; D016612:Paraffin Embedding; D018630:Vitreoretinopathy, Proliferative / Q000473:pathology*; D014822:Vitreous Body / Q000473:pathology*