[Functional expression of human GM-CSF receptor in NIH3T3 cells].
OBJECTIVE
To characterize human GM-CSF receptor(GM-R) reconstituted in NIH3T3 cells.


METHODS
Mouse NIH3T3 cells which do not express human GM-R were transfected with cDNAs encoding the alpha and beta subunit of the human GM-R, and then ligand-stimulated proliferative signal transduction and tyrosine phosphorylation of the positive transfectants were examined.


RESULTS
The reconstituted functional receptor, GM-R alpha/beta, could mediate cell proliferation and colony formation in soft agar, and induce tyrosine phosphorylation of beta c, Jak2, Shc and Shc-associated P145(SHIP). However, human GM-CSF did not activate mitogenic signal expression of NIH3T3 cells transfected with GM-R alpha alone.


CONCLUSIONS
Introduction of both alpha and beta subunit of human GM-R into NIH3T3 cells can result in ligand-dependent cell growth and colony formation via phosphorylation of beta c and activation of Jak2, Shc and SHIP signal transduction pathway.
Zhang
R
R
Jiangsu Institute of Hematology, First Affiliated Hospital, Suzhou Medical College, Suzhou 215006.
Feng
Y
Y
Zhu
Z
Z
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D051379:Mice; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection
D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor