Striatin, a calmodulin-dependent scaffolding protein, directly binds caveolin-1.
Caveolins are scaffolding proteins able to collect on caveolae a large number of signalling proteins bearing a caveolin-binding motif. The proteins of the striatin family, striatin, SG2NA, and zinedin, are composed of several conserved, collinearly aligned, protein-protein association domains, among which a putative caveolin-binding domain [Castets et al. (2000) J. Biol. Chem. 275, 19970-19977]. They are associated in part with membranes. These proteins are mainly expressed within neurons and thought to act both as scaffolds and as Ca(2+)-dependent signalling proteins [Bartoli et al. (1999) J. Neurobiol. 40, 234-243]. Here, we show that (1) rat brain striatin, SG2NA and zinedin co-immunoprecipitate with caveolin-1; (2) all are pulled down by glutathione-S-transferase (GST)-caveolin-1; (3) a fragment of recombinant striatin containing the putative caveolin-binding domain binds GST-caveolin-1. Hence, it is likely that the proteins of the striatin family are addressed to membrane microdomains by their binding to caveolin, in accordance with their putative role in membrane trafficking [Baillat et al. (2001) Mol. Biol. Cell 12, 663-673].
Gaillard
S
S
FRE 21 02, CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 092, France.
Bartoli
M
M
Castets
F
F
Monneron
A
A
D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D051242:Caveolin 1; D022461:Caveolins / Q000276:immunology / Q000378:metabolism*; D002793:Cholic Acids / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D001324:Autoantigens; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; C495537:Cav1 protein, rat; D051242:Caveolin 1; D022461:Caveolins; D002793:Cholic Acids; D003902:Detergents; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C491925:STRN protein, human; C114095:Strn protein, rat; C485774:Strn3 protein, rat; C411649:zinedin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate