[Detection of aberrant chromosomes in acute lymphoblastic leukemia by fluorescence in situ hybridization].
OBJECTIVE
To investigate the value of fluorescence in situ hybridization (FISH) in detecting aberrant chromosomes in acute lymphoblastic leukemias.


METHODS
Conventional banding techniques and FISH were used to detect trisomy 17, 18 and 21 in 38 patients with acute lymphoblastic leukemia. Lymphoid DNA probes specific for chromosomes no. 17, 18 and 21 were used for FISH.


RESULTS
Out of the 38 patients, 18 had + 17, 22 + 18 and 23 + 21. The number of trisomy cells ranged from 7% to 80%. Ten normal bone marrow samples were hybridized with the chromosomes 17, 18 and 21 probes.


CONCLUSIONS
FISH is more sensitive than conventional cytogenetic analysis and allows systematic study on large cohort of patients without the need of metaphase preparations.
Yang
K
K
Beijing Army General Hospital of PLA, Beijing 100700.
Huang
L
L
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014314:Trisomy; D014407:Tumor Cells, Cultured