title: Intercellular transfer and supramolecular organization of human leukocyte antigen C at inhibitory natural killer cell immune synapses.
abstract: After accumulation of target cell human leukocyte antigen (HLA)-C at inhibitory natural killer (NK) cell immune synapses, some HLA-C transfers from target cells to NK cell plasma membranes and cytoplasm. This unexpected intercellular transfer of HLA-C is dependent on NK receptor recognition, since HLA-Cw6 or -Cw4 but not -Cw3 transfer to an NK transfectant expressing killer Ig-like receptor (KIR)2DL1. Strikingly, live-cell time-lapse laser scanning confocal microscopy shows vesicles containing target cell green fluorescent protein-tagged HLA-C migrating away from immune synapses into NK cells. Unlike clustering of HLA-C at the immune synapse, intercellular transfer of HLA-C is dependent on NK cell ATP, but not target cell ATP. However, the intercellular transfer of HLA-C is not dependent on active polymerization of the actin cytoskeleton. In addition, different arrangements of HLA-C are seen at inhibitory NK immune synapses, and these alter as NK synapses mature, but in a fashion distinct from that seen upon T cell activation.
authors.affiliation: Department of Biological Sciences, Sir Alexander Fleming Building, Imperial College of Science, Technology, and Medicine, South Kensington, London SW7 2AZ, United Kingdom.
authors.forename: L M
authors.initials: LM
authors.lastname: Carlin
authors.forename: K
authors.initials: K
authors.lastname: Eleme
authors.forename: F E
authors.initials: FE
authors.lastname: McCann
authors.forename: D M
authors.initials: DM
authors.lastname: Davis
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001692:Biological Transport; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D015236:HLA-C Antigens / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D054342:Receptors, KIR2DL1; D013569:Synapses / Q000378:metabolism*
chemical_list: D015236:HLA-C Antigens; D011971:Receptors, Immunologic; D054342:Receptors, KIR2DL1; D000255:Adenosine Triphosphate