Interaction of alpha-2-macroglobulin and HSV-1 during infection of neuronal cells.
We describe the effect of pretreatment with alpha-2-macroglobulin (A2M) on the susceptibility of the human neuroblastoma SKNMC cell line to infection by herpes virus type 1 (HSV-1). ELISA and co-immunoprecipitation experiments confirmed the A2M-HSV-1 interaction in vitro. Indirect immunofluorescence shows that A2M exacerbated the cytopathic effect induced after HSV-1 infection. However, A2M-pretreated SKNMC cells notably produced fewer HSV-1 particles than did the untreated cells, suggesting that A2M could induce a restrictive infection. Furthermore, high levels of HSV-1 and A2M induced the production of nitric oxide (NO) in SKNMC. Preliminary results suggest that A2M might induce apoptosis in HSV-1-infected cells. These findings affirm the conclusion that A2M may interact directly with HSV-1 and modulate the course of the infection in SKNMC human neuroblastoma cells.
Alonso
M
M
Center of Molecular Biology Severo Ochoa, Universidad Autónoma de Madrid, Cantoblanco, 28040 Madrid, Spain.
Dimitrijevic
A
A
Recuero
M
M
Serrano
E
E
Valdivieso
F
F
López-Guerrero
J A
JA
D017209:Apoptosis; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006561:Herpes Simplex / Q000276:immunology* / Q000473:pathology; D018259:Herpesvirus 1, Human; D006801:Humans; D066298:In Vitro Techniques; D009447:Neuroblastoma; D009474:Neurons / Q000473:pathology / Q000821:virology*; D009569:Nitric Oxide / Q000378:metabolism; D011485:Protein Binding; D014407:Tumor Cells, Cultured / Q000821:virology; D014771:Virion / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism / Q000494:pharmacology*
D000511:alpha-Macroglobulins; D009569:Nitric Oxide