title: The immunosuppressive activity of chemically modified lipopolysaccharide of Shigella sonnei.
abstract: In the present investigations we aimed to study the effect of Shigella sonnei lipopolysaccharide (LPS) on delayed type hypersensitivity (DTH) to non-bacterial antigen in CBA mice. These experiments showed that intraperitoneal injection of phenol-water extracted LPS and avirulent S. sonnei did not affect the level of DTH. However, an injection of avirulent bacteria and LPS treated with 2-mercaptoethanol reduced significantly the levels of DTH. Gel filtration of redox-reactivated LPS through Sephadex G-200 shows that LPS contains three immunosuppressive components: approximately 800 kDa and higher, 150-200 and 50-70 kDa. These components differed by their specificity and heat-sensitivity.
authors.affiliation: Laboratory of Enteric Infections, Kiev Institute of Epidemiology and Infectious Diseases, spusk Protasov Yar 4, 02038, Kiev, Ukraine. anders.b.wikstrom@se.abb.com
authors.forename: Elena V
authors.initials: EV
authors.lastname: Vikström
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D002850:Chromatography, Gel; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D008623:Mercaptoethanol / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D010084:Oxidation-Reduction; D019800:Phenol; D012764:Shigella sonnei / Q000737:chemistry / Q000276:immunology; D013696:Temperature; D014238:Trichloroacetic Acid
chemical_list: D000941:Antigens; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D019800:Phenol; D014238:Trichloroacetic Acid; D008623:Mercaptoethanol