Role of beta-oxidation enzymes in gamma-decalactone production by the yeast Yarrowia lipolytica.
Some microorganisms can transform methyl ricinoleate into gamma-decalactone, a valuable aroma compound, but yields of the bioconversion are low due to (i) incomplete conversion of ricinoleate (C(18)) to the C(10) precursor of gamma-decalactone, (ii) accumulation of other lactones (3-hydroxy-gamma-decalactone and 2- and 3-decen-4-olide), and (iii) gamma-decalactone reconsumption. We evaluated acyl coenzyme A (acyl-CoA) oxidase activity (encoded by the POX1 through POX5 genes) in Yarrowia lipolytica in lactone accumulation and gamma-decalactone reconsumption in POX mutants. Mutants with no acyl-CoA oxidase activity could not reconsume gamma-decalactone, and mutants with a disruption of pox3, which encodes the short-chain acyl-CoA oxidase, reconsumed it more slowly. 3-Hydroxy-gamma-decalactone accumulation during transformation of methyl ricinoleate suggests that, in wild-type strains, beta-oxidation is controlled by 3-hydroxyacyl-CoA dehydrogenase. In mutants with low acyl-CoA oxidase activity, however, the acyl-CoA oxidase controls the beta-oxidation flux. We also identified mutant strains that produced 26 times more gamma-decalactone than the wild-type parents.
Waché
Y
Y
Laboratoire de Biotechnologie (équipe IMSA), Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, 21000 Dijon, France. ywache@u-bourgogne.fr
Aguedo
M
M
Choquet
A
A
Gatfield
I L
IL
Nicaud
J M
JM
Belin
J M
JM
D000214:Acyl Coenzyme A / Q000378:metabolism; D003470:Culture Media; D007783:Lactones / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D000214:Acyl Coenzyme A; D003470:Culture Media; D007783:Lactones; D012277:Ricinoleic Acids; C005235:methyl ricinoleate; D010088:Oxidoreductases