Quercetin prevents glutathione depletion induced by dehydroascorbic acid in rabbit red blood cells.
Exposure of rabbit red blood cells to dehydroascorbic acid (DHA) caused a significant decline in glutathione content which was largely prevented by quercetin, whereas it was insensitive to various antioxidants, iron chelators or scavengers of reactive oxygen species. This response was not mediated by chemical reduction of either extracellular DHA or intracellular glutathione disulfide. In addition, the flavonoid did not affect the uptake of DHA or its reduction to ascorbic acid. Rather, quercetin appeared to specifically stimulate downstream events promoting GSH formation.
Fiorani
M
M
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Via Saffi, 2, 61029 Urbino, Italy. m.fiorani@uniurb.it
De Sanctis
R
R
Menghinello
P
P
Cucchiarini
L
L
Cellini
B
B
Dachà
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000378:metabolism* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism / Q000494:pharmacology; D010084:Oxidation-Reduction; D011794:Quercetin / Q000378:metabolism* / Q000494:pharmacology; D011817:Rabbits
D011794:Quercetin; D005978:Glutathione; D001205:Ascorbic Acid; D019803:Glutathione Disulfide; D003683:Dehydroascorbic Acid