Comparison of two blastocyst culture systems: coculture on Vero cells and sequential media.
OBJECTIVE
To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period.


METHODS
Retrospective study.


METHODS
Institutional practice assisted reproductive technology center.


METHODS
Ninety-nine selected patients undergoing IVF with blastocyst culture.


METHODS
In vitro fertilization and transfer of at least one expanded blastocyst after culture using either Vero cells or sequential media (S(2) medium).


METHODS
Blastocyst, pregnancy, and implantation rates.


RESULTS
The blastocyst formation rate (41.6% vs. 31.6%) is significantly increased with sequential media compared with the coculture system, but there is no statistically significant difference in the pregnancy and implantation rates (39.5% vs. 38.6% and 23.7% vs. 15.1%, respectively).


CONCLUSIONS
Blastocyst culture on sequential media offers a better dynamic of embryo development (22% vs. 13.6% blastocyst at day 5). There was no statistically significant difference between the two groups in the take-home baby rate (18 vs. 12).
d'Estaing
S G
SG
Département de Médecine de la Reproduction, Centre hospitalier de l'Université de Biologie de la reproduction, Hôpital Edouard Herriot, Lyon, France. sge@rockefeller.univ-lyon1.fr
Lornage
J
J
Hadj
S
S
Boulieu
D
D
Salle
B
B
Guérin
J F
JF
D000328:Adult; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000187:drug effects / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D014709:Vero Cells
D003470:Culture Media