Semiquantitative expression analysis of ephrine-receptor tyrosine kinase mRNA's in a rat model of traumatic brain injury.
The molecular mechanisms involved in recovery of function of the central nervous system (CNS) after injury to the brain are incompletely understood. Here the expression of ephrine (Eph) kinases following traumatic brain injury (subdural haematoma) was analysed in order to find out whether these developmentally regulated genes may be involved in tissue remodelling after brain damage. mRNA was isolated from ipsilateral cortices 7, 18, and 28 days after surgery and semiquantitative reverse transcription-polymerase chain reaction was performed. Most Eph kinases did not show significant regulation at gene expression level during the time course of recovery from acute brain injury but there is some evidence that mRNA of EphB1 might be slightly upregulated.
Biervert
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C
CNS Research, Bayer AG, Wuppertal, Germany.
Horvath
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E
Fahrig
T
T
D000818:Animals; D005326:Fetal Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006408:Hematoma, Subdural / Q000378:metabolism*; D008297:Male; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D036104:Receptor, EphA2; D036122:Receptor, EphA4; D036123:Receptor, EphA5; D036141:Receptor, EphA7; D036183:Receptor, EphB2; D036224:Receptor, EphB4; D036261:Receptor, EphB6; D036081:Receptors, Eph Family
D005326:Fetal Proteins; D009841:Oligonucleotides; D012333:RNA, Messenger; D036261:Receptor, EphB6; D020794:Receptor Protein-Tyrosine Kinases; D036104:Receptor, EphA2; D036122:Receptor, EphA4; D036123:Receptor, EphA5; D036141:Receptor, EphA7; D036183:Receptor, EphB2; D036224:Receptor, EphB4; D036081:Receptors, Eph Family