Up-regulation of phosphatidylinositol 3-kinase in psoriatic lesions.
OBJECTIVE
To explore the relationship between phosphatidylinositol 3-kinase (PI 3-kinase) and psoriasis.


METHODS
We constructed eukaryotic expression vector PcDNA 3-PI 3-kinase, by which digoxin-labeled RNA probe of PI 3-kinase was generated. After that, we detected the expressions of PI 3-kinase in the skins of twelve patients with plaque psoriasis and five normal subjects by dot blot, in situ hybridization and immunohistochemistry.


RESULTS
The expression of PI 3-kinase mRNA and protein in psoriatic lesions increased obviously compared with that in normal skin. The expression of the PI 3-kinase gene conformed to the expression of its protein in psoriatic and normal human skins.


CONCLUSIONS
Over-expression of PI 3-kinase may be related to the hyperproliferation of psoriatic keratinocytes. Transfection of the anti-sense gene of PI 3-kinase into psoriatic keratinocytes might be a best way to elucidate the issue.
Zhang
X
X
Department of Biology, Beijing Normal University, Beijing 100875, China.
Liu
H
H
Ma
S
S
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007223:Infant; D015603:Keratinocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis* / Q000235:genetics; D011565:Psoriasis / Q000201:enzymology* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000201:enzymology* / Q000473:pathology; D015854:Up-Regulation
D012333:RNA, Messenger; D019869:Phosphatidylinositol 3-Kinases