Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection.
Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.
Ruan
Y
Y
Department of Otolaryngology, Yamanashi Medical University, Yamanshi, Japan.
Okamoto
Y
Y
Matsuzaki
Z
Z
Endo
S
S
Matsuoka
T
T
Kohno
T
T
Chazono
H
H
Eiko
I
I
Tsubota
K
K
Saito
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D053222:Fas Ligand Protein; D018928:Immunity, Mucosal; D020287:In Situ Nick-End Labeling; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000276:immunology / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000821:virology; D012136:Respiratory Syncytial Viruses / Q000254:growth & development / Q000302:isolation & purification; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014779:Virus Replication / Q000276:immunology
D000911:Antibodies, Monoclonal; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D016753:Interleukin-10