Fourier-transform infrared spectroscopic study of globulin from Phaseolus angularis (red bean).
The conformation of red bean globulin dispersions (approximately 10% in D2O or deuterated phosphate buffer pD 7.4) under the influence of pH, chaotropic salts, protein structure perturbants, and heating conditions was studied by Fourier-transform infrared (FTIR) spectroscopy. The FTIR spectrum of red bean globulin showed major bands from 1682 to 1637 cm(-1) in the amide I' region, corresponding to the four types of secondary structures, i.e. beta-turns, beta-sheets, alpha-helix and random coils. At extreme pH conditions, there were changes in intensity in bands attributed to beta-sheet (1637 and 1618 cm(-1)) and random coil (1644 cm(-1)) structures, and shifts of these bands to lower or higher wave numbers, indicating changes in protein conformation. Chaotropic salts caused progressive increases in random coil structures and concomitant decreases in beta-sheet bands, following the lyotrophic series of anions. In the presence of sodium dodecyl sulfate and ethylene glycol, pronounced increases in the random coil band were observed, accompanied by slight shifts of the beta-sheet band. Addition of dithiothreitol and N-ethylmaleimide did not cause marked changes in the FTIR spectra. Heating at increasing temperature led to progressive decreases in the intensity of the alpha-helix and beta-sheet bands and increases in random coil band intensity, leveling off at around 60 degrees C. The data suggest that re-organization of protein structure occurred at temperatures well below the denaturation temperature of red bean globulin (86 degrees C) as determined by differential scanning calorimetry. This was accompanied by pronounced increases in the intensity of the two intermolecular beta-sheet bands (1682 and 1619-1620 cm(-1)) associated with the formation of aggregated strands at higher temperatures (80-90 degrees C). Increases in intensity of the aggregation bands were also observed in the heat-induced buffer-soluble and insoluble aggregates.
Meng
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Food Science Laboratory, Department of Botany, The University of Hong Kong, Polfulam Road, Pokfulam, Hong Kong.
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D002152:Calorimetry, Differential Scanning; D004229:Dithiothreitol / Q000494:pharmacology; D019855:Ethylene Glycol / Q000737:chemistry; D005033:Ethylmaleimide / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry*; D005916:Globulins / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012492:Salts / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014867:Water / Q000737:chemistry
D005916:Globulins; D012492:Salts; D014867:Water; D012967:Sodium Dodecyl Sulfate; D019855:Ethylene Glycol; D005033:Ethylmaleimide; D004229:Dithiothreitol