title: Deletion and dosage modulation of the eEF1A gene in Podospora anserina: effect on the life cycle.
abstract: eEF1A is encoded by a unique gene in the filamentous fungus Podospora anserina. We show here that (1) this gene is essential for vegetative growth, (2) readthrough at UGA stop codon level is positively correlated with eEF1A level, (3) eEF1A level is regulated in P. anserina. (4) Increasing eEF1A gene dosage does not modify P. anserina life cycle parameters, especially longevity is not changed. These data confirm and extend those previously obtained in yeast and Drosophila.
authors.affiliation: Institut de Génétique et Microbiologie, l'Université de Paris-Sud, C.N.R.S. UMR 8621, Bât. 400, 91405 Orsay, France. silar@igmors.u-psud.fr
authors.forename: P
authors.initials: P
authors.lastname: Silar
authors.forename: M
authors.initials: M
authors.lastname: Rossignol
authors.forename: V
authors.initials: V
authors.lastname: Haedens
authors.forename: Z
authors.initials: Z
authors.lastname: Derhy
authors.forename: A
authors.initials: A
authors.lastname: Mazabraud
mesh_terms: D017353:Gene Deletion; D018628:Gene Dosage; D005800:Genes, Fungal / Q000502:physiology*; D016296:Mutagenesis; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020037:Sordariales / Q000235:genetics / Q000254:growth & development*
chemical_list: D020648:Peptide Elongation Factor 1