Expression of GABA(B1) and GABA(B2) mRNA in the human brain.
GABA(B) receptors are widely expressed in the CNS. The distribution of the recently cloned GABA(B1) receptor is highly concordant with GABA(B) ligand binding sites, but experiments with transfected cell lines indicate that GABA(B1) has to heterodimerize with GABA(B2) to gain the functionality of a native GABA(B) receptor. Using in situ hybridization we investigated the expression of GABA(B1) and GABA(B2) mRNAs in the human brain. Both transcripts were detectable in prefrontal cortex, hippocampus and cerebellum with no apparent mismatch. A distinct expression of GABA(B1) was detected in putative Bergmann glia. In the human striatum GABA(B1) mRNA was expressed in moderate amounts, whereas the GABA(B2) mRNA signal was not clearly above background. According to our data, the current concept on GABA(B) receptor composition needs re-evaluation, at least for certain brain structures.
Berthele
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Department of Neurology, Technical University, Moehlstrasse 28, 81675 Munich, Germany.
Platzer
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Weis
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Conrad
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Tölle
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D000368:Aged; D001479:Basal Ganglia / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009433:Neural Inhibition / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D017397:Prefrontal Cortex / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D018079:Receptors, GABA / Q000235:genetics*; D018080:Receptors, GABA-B / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
C485503:GABBR2 protein, human; D012333:RNA, Messenger; D018079:Receptors, GABA; D018080:Receptors, GABA-B; D005680:gamma-Aminobutyric Acid