Micellar electrokinetic chromatography separation and laser-induced fluorescence detection of the lipid peroxidation product 4-hydroxynonenal.
4-Hydroxnonenal (HNE) is a product of lipid peroxidation in biological systems that causes a variety of harmful biological effects. A method for identifying HNE based on derivatization with the fluorescent reagent dansylhydrazine (5-(dimethylamino)naphthalene-1-sulphonehydrazine (DNSH) followed by micellar electrokinetic chromatography separation laser-induced fluorescence detection has been developed. The derivatization reaction has also been investigated for significant experimental parameters and rat brain homogenates with induced lipid peroxidation have been analysed for HNE contents. The limit of detection (3 S/N) was 30 nM or 0.3 fmol in the injected sample.
Claeson
K
K
Department of Analytical Chemistry, Stockholm University, Sweden.
Thorsén
G
G
Karlberg
B
B
D000447:Aldehydes / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000378:metabolism; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D007834:Lasers; D015227:Lipid Peroxidation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000447:Aldehydes; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D006834:Hydrazines; C451039:dansylhydrazine-5-(dimethylamino)naphthalene-1-sulphonehydrazine; C027576:4-hydroxy-2-nonenal