[Human interleukin-13 cDNA cloning and expression].
OBJECTIVE
To construct an efficient expression system for HIL-13 in prokaryotic cells.


METHODS
After Amplified by RT-PCR, The cDNA fragment encoding HIL-13 was inserted into pGEX-2T plasmid and expressed under induced condition.


RESULTS
HIL-13 cDNA was obtained. Recombinant plasmid pGEX-2T HIL-13 was constructed and sequenced, the inserted fragment of the recombinant plasmid was confirmed to be HIL-13 cDNA. HIL-13 was expressed as a fusion protein with glutathione-s-transferase (GST-HIL-13, MW = 39,000).


CONCLUSIONS
(1) HIL-13 cDNA has been obtained from T lymphocytes. (2) The rate of expression of HIL-13 in prokaryotic cells is about 37%.
Sun
Z
Z
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Si
J
J
Liu
S
S
Song
G
G
Zheng
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017421:Sequence Analysis
D018076:DNA, Complementary; D018793:Interleukin-13; D011993:Recombinant Fusion Proteins