title: Suppression of apoptotic and necrotic cell death by poliovirus.
abstract: To determine an antiapoptotic activity of poliovirus type 1 (PV-1), we examined the effect of PV-1 infection on apoptosis that was induced in HEp-2 cells by the treatment with 1 M sorbitol. The virus did not induce apoptosis in the infected cells and could suppress both the fragmentation of chromosomal DNA and morphological cell and cell nuclei changes in the sorbitol-treated cells, indicating that PV-1 induces an antiapoptotic state. Comparison of the kinetics showed that this ability of the virus appeared in the infected cells at the time of progeny virus formation (maturation step of virus multiplication). Simultaneously with this antiapoptotic activity, PV-1 infection also suppressed non-apoptotic cell death induced by sodium chloride. Electron microscopic observation revealed that the cells killed by the sodium chloride treatment had undergone liquefactive necrosis, indicating that PV-1 can inhibit both apoptosis and necrosis. In addition, PV-1 can grow in the apoptotic cells, although the virus yield was reduced to a quarter of the yield in normal cells.
authors.affiliation: Department of Virology1 and Department of Ophthalmology3, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.
authors.forename: A Hajime
authors.initials: AH
authors.lastname: Koyama
authors.affiliation: Department of Pathology, Teikyo University School of Medicine, Kaga, Itabashi-ku, Tokyo 173-8650, Japan2.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Irie
authors.affiliation: Department of Virology1 and Department of Ophthalmology3, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ueno
authors.affiliation: Department of Virology1 and Department of Ophthalmology3, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.
authors.forename: Motomi
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyou-ku, Tokyo 113-0033, Japan4.
authors.forename: Akio
authors.initials: A
authors.lastname: Nomoto
authors.affiliation: Department of Virology1 and Department of Ophthalmology3, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Adachi
mesh_terms: D017209:Apoptosis; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D005057:Eukaryotic Cells / Q000473:pathology / Q000821:virology; D006801:Humans; D009336:Necrosis; D017955:Poliovirus / Q000502:physiology*; D011533:Proviruses / Q000302:isolation & purification / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D014779:Virus Replication
chemical_list: D012965:Sodium Chloride; D013012:Sorbitol