Induction of morphological deformities in Chironomus tentans exposed to zinc- and lead-spiked sediments.
Laboratory experiments were used to assess morphological responses of Chironomus tentans larvae exposed to three levels of zinc and lead. Chironomus tentans egg masses were placed into triplicate control and metal-spiked aquaria containing the measured concentrations 1,442, 3,383, and 5,562 microg/g Pb dry weight and 1,723, 3,743, and 5,252 microg/g Zn dry weight. Larvae were collected at 10-d intervals after egg masses were placed in aquaria until final emergence. Larvae were screened for mouthpart deformities and metal body burdens. Deformities increased with time of exposure in both Zn and Pb tanks. Deformity rates between the three Zn concentrations differed statistically, with low and medium Zn levels containing the highest overall deformity rates of 12%. Deformity rates for larvae held in the Pb aquaria were found to differ significantly. Larvae in the low-Pb tanks had a deformity rate of 9%. Larvae and water from both the Zn and Pb aquaria had increasing metal concentrations with increasing sediment metal concentration. Results demonstrate that Zn and Pb each induce chironomid mouthpart deformities at various concentrations. However, a clear dose-related response was not demonstrated. Our research provides more support for the potential use of chironomid deformities as a tool for the assessment of heavy metal pollution in aquatic systems.
Martinez
A
A
Washington State University, Department of Natural Resource Sciences, Pullman 99164-6410, USA.
Moore
B C
BC
Schaumloffel
J
J
Dasgupta
N
N
D000818:Animals; D002683:Chironomidae / Q000187:drug effects / Q000254:growth & development*; D000013:Congenital Abnormalities / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry; D007814:Larva / Q000254:growth & development; D007854:Lead / Q000009:adverse effects*; D018675:Toxicity Tests; D014873:Water Pollutants / Q000009:adverse effects*; D015032:Zinc / Q000009:adverse effects*
D014873:Water Pollutants; D007854:Lead; D015032:Zinc