Estimation of the antioxidant activities of flavonoids from their oxidation potentials.
A simple electrochemical method for estimating the antioxidant activity (AA) of flavonoids has been developed. The proposed method is based on a measurement of the half-wave potential (E1/2) of the first oxidation wave of flavonoids by using flow-through column electrolysis. At the same time, the lipid peroxidation (LPO) inhibiting effects of these flavonoids were determined. A quantitative structure-activity relationship was obtained to describe the AA of flavonoids: IC50(microM) = 30.36 + 151.50 E1/2 (V) -12.63 log P (r = 0.852), where IC50 represents the concentration for 50% inhibition of LPO, and P represents the octanol/water partition coefficient. This method is expected to be useful for the quick screening of flavonoid antioxidants, and evaluating the AA of flavonoid-containing foods and medicinal plants.
Yang
B
B
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, 100700, China.
Kotani
A
A
Arai
K
K
Kusu
F
F
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D004572:Electrolysis; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D005504:Food Analysis; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010946:Plants, Medicinal; D021281:Quantitative Structure-Activity Relationship; D051381:Rats
D000975:Antioxidants; D005419:Flavonoids