A capillary electrophoretic system with an improved gas-phase chemiluminescence nitrogen detector.
A new, rugged nebulizer interface was designed to couple a capillary electrophoretic system to a gas-phase nitrogen chemiluminescence detector. By hydraulically decoupling the separation capillary from the nebulizer of the gas-phase nitrogen chemiluminescence detector, the interface-related loss of separation efficiency could be limited to a mere 10%. The ozone reaction chamber of the gas-phase nitrogen chemiluminescence detector has been redesigned leading to a threefold improvement in detection sensitivity over the old design. In combination with a field-amplified sample stacking procedure, the new system was used to detect 0.25 ppm concentrations of UV chromophore-free aminoglycosides.
Sokolowski
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AD
Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
Vigh
G
G
D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D008163:Luminescent Measurements; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000617:Aminoglycosides