[The effect of the macrophages conditioned media(MOCM) on NGF secretion in newborn rat schwann cells in vitro].
OBJECTIVE
To study the influence of macrophages on nerve growth factor (NGF) secretion in newborn rat schwann cells.


METHODS
LPS-activated and not-activated adult rat peritoneal macrophages conditioned media (MOCM) were treated on purified cultured Schwann cells for 24, 48 and 72 hours in three different concentrations the NGF contents in the cultured Schwann cells suspension media were measured by a two-site enzyme-linked immunoserbent assay.


RESULTS
Both L-MOCM (LPS-activated MOCM) and N-MOCM(not-activated MOCM) obviously enhanced NGF secretion in chwann cells in each different concentration or testing time.


CONCLUSIONS
MOCM contains some factors which can promote NGF secretion in rat schwann cells in vitro, suggesting that macrophages regulating NGF secretion by secreted certain cytokines.
Ma
X
X
Department of Anatomy, First Military Medical University, Guangzhou 510515.
Yu
Y
Y
Zhong
S
S
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000378:metabolism*
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D020932:Nerve Growth Factor