[Different expressions of cyclins and cyclin dependent kinase inhibitor P27 in vascular smooth muscle cells and cardiac myocytes caused by angiotensin II and PDGF stimulation].
OBJECTIVE
To investigate cell cycle distribution of the vascular smooth muscle cells (VSMC) and cardiac myocytes(CM) and different expressions of cyclins and P27 caused by angiotensin II (Ang II) and platelet derived growth factor BB (PDGF-BB).


METHODS
Cultured VSMCs or CMs were collected at 24 h after stimulation of Ang II (10(-6) mol/L) and PDGF-BB (20 ng/ml). Cell cycle distribution, cyclins and P27 expression were determined with flow cytometer.


RESULTS
Ang II had no effect on P27 expression in both VSMCs and CMs. It did not cause proliferation of both VSMCs and CMs. Although PDGF-BB could significantly increase cyclins expression in both VSMCs and CMs, it could only significantly inhibit P27 expression in VSMCs (1.8 +/- 1.3 vs 48.1 +/- 5.4, P < 0.01) and cause VSMC proliferation, and had no effect on P27 expression in CMs(39.4 +/- 5.6 vs 44.6 +/- 3.6, P > 0.05).


CONCLUSIONS
The progression of VSMC and CM through G1 to S phase might be brought out by the inhibition of P27 during proliferation.
Xu
D
D
Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Yuan
Y
Y
Liu
Y
Y
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000378:metabolism; D000077214:Becaplermin; D002478:Cells, Cultured; D016213:Cyclins / Q000096:biosynthesis*; D008297:Male; D008840:Microfilament Proteins / Q000096:biosynthesis*; D009124:Muscle Proteins; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley
D000925:Anticoagulants; D016213:Cyclins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; C058992:Tagln protein, mouse; D000804:Angiotensin II; D000077214:Becaplermin