[Effects of defibrinogenase on photochemically induced middle cerebral artery occlusion in stroke-prone renovascular hypertensive rats].
OBJECTIVE
To investigate the effects of intravenous injection of defibrinogenase on dissoliving dissolve thrombus and salvaging cerebral infarction in stroke-prone renovascular hypertension rats (RHRSP) with middle cerebral artery occlusion(MCAO).


METHODS
The MCAO in RHRSP was induced by administration in photosensitive dye, rose bengal, and irradiation of the main MCA with a focus laser beam. The thrombus in MCA and cerebral infarction were examined microscopically in HE stained sections. Infarction size was determined in triphenyltetrazolium chloride stained sections. Defibrinogenase was injected intravenously at 3, 6, 9, 12 hours and 3, 7 days after MCAO, meanwhile the control group received normal saline. All rats were killed at 72 hours and evaluated by pathological methods after treatment.


RESULTS
In the treatment group, the thrombi were dissolved totally at 3 hours after MCAO, 75% at 6 hours, 13% at 9 hours. Defibrinogenase failed to dissolve thrombus beyond 12 hours after MCAO, just as control group. In comparison with the control group, defibrinogenase significantly reduced infarction size. There was no evidence of hemorrhage.


CONCLUSIONS
Defibrinogenase can reduce infarction size and dissolve thrombus in 6 hours after MCAO without the side effect of hemorrhage.
Huang
R
R
Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China.
Zeng
J
J
Pei
Z
Z
D000721:Ancrod / Q000627:therapeutic use*; D000818:Animals; D005343:Fibrinolytic Agents / Q000627:therapeutic use*; D006978:Hypertension, Renovascular / Q000150:complications; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D010777:Photochemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015912:Thrombolytic Therapy
D005343:Fibrinolytic Agents; D000721:Ancrod