Deglycosylated mucin as a substrate in enzymatic O-glycosylation in vitro.
To obtain the apomucin as a substrate for glycosyltransferases activity testing, the pig gastric mucin deglycosylation by chemical method was carried out. Resulted apomucin, rich in Ser, Thr and Pro residues, was as good carbohydrate acceptor in enzymatic O-glycosylation in vitro, as synthetic peptide, analogue of MUC2 mucin tandem repeats sequence.
Paszkiewicz-Gadek
A
A
Department of General and Organic Chemistry, Medical Academy of Białystok.
Porowska
H
H
Kisiel
D
D
Gindzieński
A
A
D000595:Amino Acid Sequence; D000818:Animals; D004187:Disaccharides / Q000138:chemical synthesis; D005752:Gastric Mucins / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D055262:Mucin-2; D009077:Mucins / Q000235:genetics; D013379:Substrate Specificity; D013552:Swine
D004187:Disaccharides; D005752:Gastric Mucins; D055262:Mucin-2; D009077:Mucins; C053366:apomucin; D016695:Glycosyltransferases