[Identification of cDNA fragments related to alpha particles radiation induced neoplastic transformation of rat tracheal epithelial cells].
OBJECTIVE
To isolate genes involved in neoplastic transformation induced by alpha-particles.


METHODS
The differential display technique was used to identify differentially expressed mRNA species between primary and neoplastic transformed rat tracheal epithelial (RTE) cells induced by alpha particles.


RESULTS
15 differentially displayed gene fragments were cloned and sequenced. Seven novel ESTs were submitted to GenBank. Homology searching revealed that clone ZG35 was highly homologous to rat Annexin I gene which was thought to be involved in mitogenic signal transduction as a substrate of epidermal growth factor receptor/kinase. Clone ZC52 was nearly identical to a novel rattus norvegicus gene for carboxylesterase precursor, clone ZG52 was 3' similar to human SRPK2 gene suggesting it may be a novel SRPK2 related gene involved in pre-mRNA splicing. Clone ZA73 was a novel gene fragments, and clone ZC66 was highly homologous to rat nucleoside diphosphate kinase gene or metastasis suppressor gene nm23, sequence analysis suggested that nm23 might be mutated in the transformed RTE cells. Northern hybridization confirmed the above gene or gene fragments to be up-regulated in the transformed RTE cells.


CONCLUSIONS
These results provide clues to elucidate the mechanisms of radiation oncogenesis and suggest that Annexin I, carboxylesterase precursor gene, ZG52 and nm23 may be involved in the neoplastic transformation induced by alpha-particles radiation.
Li
G
G
Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850.
Wu
D
D
Hu
Y
Y
D000512:Alpha Particles; D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000473:pathology* / Q000528:radiation effects; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012326:RNA Splicing; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014132:Trachea / Q000166:cytology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D017305:Annexin A1; D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; C110522:SRPK2 protein, human; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins