title: Dependence of erythrocyte vesiculation and hemolysis parameters on the concentration of sodium dodecyl sulfate. Vesicular-competitive hemolysis.
abstract: The kinetic and concentration dependences of erythrocyte vesiculation and hemolysis induced by sodium dodecyl sulfate were studied. The similarity of the slopes of the dose dependence of the SDS-induced vesiculation and slow hemolysis rates in the double logarithmic coordinates suggested a close relation between the processes of vesiculation and pore formation for slow hemolysis by the detergent. Further evidence of the competitive nature of the detergent-induced vesiculation and fast hemolysis by sodium dodecyl sulfate was obtained. The phenomenon of partial hemolysis proceeding at a rate comparable to that of cell vesiculation is explained in terms of the competition between hemolysis and vesiculation, without resorting to erythrocyte heterogeneity. New vesicular-competitive hemolysis is described. Based on it, the action of different hemolysis-inducing agents is analysed.
authors.affiliation: Institute of Photobiology, National Academy of Sciences of Belarus, Minsk, Republic of Belarus. sei@biobel.bas-net.by
authors.forename: E A
authors.initials: EA
authors.lastname: Chernitsky
authors.forename: O A
authors.initials: OA
authors.lastname: Senkovich
authors.forename: V V
authors.initials: VV
authors.lastname: Rozin
mesh_terms: D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*
chemical_list: D003902:Detergents; D012967:Sodium Dodecyl Sulfate