title: Immortalized Epstein-Barr virus-positive B-cell lines obtained by prolonged culture of peripheral blood mononuclear cells from human immunodeficiency virus type 1-positive patients.
abstract: OBJECTIVE
  To study the factors that determine malignant B cell growth in human immunodeficiency virus type 1 (HIV-1)-infected patients.
  
  
  METHODS
  B-cell lines (lymphocyte cell lines [LCL]) were developed after nonstimulated culture of peripheral blood mononuclear cells (PBMC) from HIV-1-positive (HIV-1(+)) patients. Human immunodeficiency virus type 1 replication in culture, Epstein-Barr virus (EBV) latent oncogene expression, and cell-to-cell interaction were studied after nonstimulated culture of HIV-1(+) PBMC, analyzing their contribution to LCL appearance.
  
  
  METHODS
  Nonstimulated PBMC cultures of HIV-1(+) PBMC and controls (N-PBMC) were established. Lymphocyte cell lines were characterized. Epstein-Barr virus latent membrane protein 1 (LMP-1) and Epstein-Barr nuclear antigen 2 were detected by polymerase chain reaction (PCR). Clonality of LCL was determined by light chain restriction (flow cytometry) and immunoglobulin H chain rearrangement (semi-nested PCR). Peripheral blood mononuclear cell phenotypes were studied at different intervals of culture.
  
  
  RESULTS
  Lymphocyte cell lines were obtained in 73% of HIV-1(+) PBMC cultures, compared with 6% in N-PBMC. All LCL were EBV-positive (EBV(+)). B-cell lineage was established, and up to 12 different B-cell clones were expanded from the same individual. Occurrence of LCL was more frequent in cultures with HIV-1 replication, high LMP-1 expression in viable B cells, and high CD4:CD8 ratio. Human immunodeficiency virus type 1 replication persisted in 53% of the LCL.
  
  
  CONCLUSIONS
  In vitro HIV-1 replication and persistence of viable EBV(+) lymphoblasts favor spontaneous in vitro outgrowth of LCL in HIV-1(+) patients.
authors.affiliation: Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina. bhruibal@mail.retina.ar
authors.forename: B
authors.initials: B
authors.lastname: Ruibal-Ares
authors.forename: L
authors.initials: L
authors.lastname: Belmonte
authors.forename: P
authors.initials: P
authors.lastname: Baré
authors.forename: M
authors.initials: M
authors.lastname: Scolnik
authors.forename: M F
authors.initials: MF
authors.lastname: Palacios
authors.forename: C
authors.initials: C
authors.lastname: Bayo-Hanza
authors.forename: C M
authors.initials: CM
authors.lastname: Galmarini
authors.forename: G
authors.initials: G
authors.lastname: Mendez
authors.forename: M M
authors.initials: MM
authors.lastname: de Bracco
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology* / Q000821:virology*; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D005434:Flow Cytometry; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006467:Hemophilia A / Q000150:complications; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology / Q000821:virology*