title: Assignment of Pseudomonas sp. strain E-3 to Pseudomonas psychrophila sp. nov., a new facultatively psychrophilic bacterium.
abstract: A facultatively psychrophilic bacterium, previously described as Pseudomonas sp. strain E-3, has been reassigned by phenotypic characterization, chemotaxonomic analysis, DNA-DNA hybridization, and 16S rRNA gene phylogenetic analysis. The organism was a gram-negative, aerobic. straight rod with polar flagella. It was catalase positive and oxidase positive, able to grow at -1 degree C but not at 40 degree C, and produced acid from D-glucose under aerobic conditions. The major isoprenoid quinone was ubiquinone-9, and the DNA G + C content was 57.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the bacterium is a member of the genus Pseudomonas and was closest to Pseudomonas fragi. Determination of the DNA-DNA relatedness between strain E-3 and P. fragi revealed too low a level of homology (47.9%-51.3%) to identify them as the same species. On the basis of phenotypic characteristics, phylogenetic analysis, and DNA-DNA relatedness data, it is concluded that strain E-3 represents an individual species. Accordingly, the name Pseudomonas psychrophila is proposed. The type strain is E-3T (= JCM 10889).
authors.affiliation: Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Tovohira-ku, Sapporo 062-8517, Japan. i.yumoto@aist.go.jp
authors.forename: I
authors.initials: I
authors.lastname: Yumoto
authors.forename: T
authors.initials: T
authors.lastname: Kusano
authors.forename: T
authors.initials: T
authors.lastname: Shingyo
authors.forename: Y
authors.initials: Y
authors.lastname: Nodasaka
authors.forename: H
authors.initials: H
authors.lastname: Matsuyama
authors.forename: H
authors.initials: H
authors.lastname: Okuyama
mesh_terms: D001482:Base Composition; D003080:Cold Temperature; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008854:Microscopy, Electron; D010641:Phenotype; D010802:Phylogeny; D011549:Pseudomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000648:ultrastructure; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S