Towards the use of baculovirus as a gene therapy vector.
The use of baculovirus vectors for gene expression in mammalian cells is in continuous expansion. These vectors do not replicate in mammalian cells, do not cause a cytopathic effect upon infection and are able to carry large DNA inserts. Baculovirus vectors have been shown to transduce various cell types in vitro and in vivo with significant efficiency leading to stable gene expression. This review focuses on recent developments with baculovirus that highlight its potential usefor new gene therapy strategies.
Pieroni
L
L
Cell Biology and Biophysics Department, Heidelberg, Germany. pieroni@embl-heidelberg.de
La Monica
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N
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002490:Central Nervous System / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods* / Q000639:trends; D005822:Genetic Vectors; D006801:Humans; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009369:Neoplasms / Q000628:therapy