Inhibition of Mycobacterium tuberculosis growth by saringosterol from Lessonia nigrescens.
Assay-guided fractionation of an antitubercular extract obtained from Lessonia nigrescens yielded the phytosterol saringosterol as its active component. No appreciable toxicity against Vero cells was observed for this compound. Saringosterol was also synthesized by oxidation of fucosterol. The MIC values for antitubercular activity of saringosterol and its 24S and 24R epimers were determined as 0.25, 1, and 0.125 microg/mL.
Wächter
G A
GA
Department of Pharmacology and Toxicology, College of Pharmacy, The University of Arizona, 1703 E. Mabel Street, Tucson, Arizona 85721-0207, USA.
Franzblau
S G
SG
Montenegro
G
G
Hoffmann
J J
JJ
Maiese
W M
WM
Timmermann
B N
BN
D000818:Animals; D000995:Antitubercular Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D002677:Chile; D002522:Chlorocebus aethiops; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D015394:Molecular Structure; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D000459:Phaeophyceae / Q000737:chemistry*; D013237:Stereoisomerism; D013265:Stigmasterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014709:Vero Cells / Q000187:drug effects
D000995:Antitubercular Agents; C442582:saringosterol; C015896:fucosterol; D013265:Stigmasterol