Induced synthesis of albumin-like protein in damaged rat reticulocytes.
In this study, we present evidence that red blood cell (RBC) membrane p68 in the Belgrade (b/b) rat is similar if not identical to rat serum albumin (RSA). Structural homology between RSA and the RBC p68 has been determined by a variety of biochemical and immunological criteria. This albumin-like protein is a normal constituent of rat RBC and it is partially exported by exosomes during erythroid differentiation. The endogenous origin of rat RBC albumin-like protein was demonstrated by monitoring protein synthesis in reticulocytes and by identification of reticulocyte mRNA for albumin. Haemolytic anaemia, either hereditary (b/b rat) or drug-induced (phenylhydrazine-treated normal rat), results in increased accumulation of the albumin-like protein in rat RBCs as a result of its induced synthesis.
Trifunović
A
A
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Zarić
J
J
Lusić
M
M
Mijatović
M
M
Glisin
V
V
Popović
Z
Z
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D000743:Anemia, Hemolytic / Q000097:blood*; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010659:Phenylhydrazines; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D012156:Reticulocytes / Q000378:metabolism*; D012709:Serum Albumin / Q000737:chemistry
D000418:Albumins; D010659:Phenylhydrazines; D012333:RNA, Messenger; D012709:Serum Albumin; C030299:phenylhydrazine