Toxicity analysis of N- and C-terminus-deleted vegetative insecticidal protein from Bacillus thuringiensis.
A vegetative insecticidal protein (VIP)-encoding gene from a local isolate of Bacillus thuringiensis has been cloned, sequenced, and expressed in Escherichia coli. The expressed protein shows insecticidal activity against several lepidopteran pests but is ineffective against Agrotis ipsilon. Comparison of the amino acid sequence with those of reported VIPs revealed a few differences. Analysis of insecticidal activity with N- and C-terminus deletion mutants suggests a differential mode of action of VIP against different pests.
Selvapandiyan
A
A
International Centre for Genetic Engineering and Biotechnology, New Delhi 110 067, India.
Arora
N
N
Rajagopal
R
R
Jalali
S K
SK
Venkatesan
T
T
Singh
S P
SP
Bhatnagar
R K
RK
D000818:Animals; D001413:Bacillus thuringiensis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D007915:Lepidoptera; D008969:Molecular Sequence Data; D010572:Pest Control, Biological; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; C447139:Vip3A protein, Bacillus thuringiensis