title: Three-dimensional structure of the photosystem II core dimer of higher plants determined by electron microscopy.
abstract: Here we report the first three-dimensional structure of a higher plant photosystem II core dimer determined by electron crystallography at a resolution sufficient to assign the organization of its transmembrane helices. The locations of 34 transmembrane helices in each half of the dimer have been deduced, 22 of which are assigned to the major subunits D1 (5), D2 (5), CP47 (6), and CP43 (6). CP47 and CP43, located on opposite sides of the D1/D2 heterodimer, are structurally similar to each other, consisting of 3 pairs of transmembrane helices arranged in a ring. Both CP47 and CP43 have densities protruding from the lumenal surface, which are assigned to the loops joining helices 5 and 6 of each protein. The remaining 12 helices within each half of the dimer are attributed to low-molecular-weight proteins having single transmembrane helices. Comparison of the subunit organization of the higher plant photosystem II core dimer reported here with that of its thermophilic cyanobacterial counterpart recently determined by X-ray crystallography shows significant similarities, indicative of a common evolutionary origin. Some differences are, however, observed, and these may relate to variations between the two classes of organisms in antenna linkage or thermostability.
authors.affiliation: Wolfson Laboratories, Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London, SW7 2AY, United Kingdom.
authors.forename: B
authors.initials: B
authors.lastname: Hankamer
authors.forename: E
authors.initials: E
authors.lastname: Morris
authors.forename: J
authors.initials: J
authors.lastname: Nield
authors.forename: C
authors.initials: C
authors.lastname: Gerle
authors.forename: J
authors.initials: J
authors.lastname: Barber
mesh_terms: D019281:Dimerization; D008854:Microscopy, Electron; D008958:Models, Molecular; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D045332:Photosystem II Protein Complex; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D018724:Spinacia oleracea / Q000737:chemistry
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex