[In situ localization of mRNA gelatinases in cerebral aneurysms].
OBJECTIVE
To explore the role of gelatinases A and B and their tissue inhibitors, TIMP-1 and TIMP-2, in the formation of cerebral aneurysm.


METHODS
In situ hybridization with digoxigenin RNA probes localized cells responsible for gelatinases A and B, TIMP-1 and TIMP-2 mRNA production in histologic sections of aneurysmal (n = 12), and normal (n = 6) cerebral arteries.


RESULTS
The levels of gelatinase B mRNA were found very high in cerebral aneurysms, especially in the site of internal elastic lamella. Gelatinase B mRNA was not found in normal cerebral arteries. Gelatinase A TIMP-1 and TIMP-2 mRNA were not found in both cerebral aneurysms and normal cerebral arteries.


CONCLUSIONS
Differential patterns of expression can be seen in situ, and tissue gelatinase B mRNA levels are elevated in aneurysms, suggesting that gelatinase B maybe responsible for the destruction of internal elastic lamina or even all laminae of cerebral arteries, leading to cerebral aneurysms.
Zhao
J
J
Department of Neurosurgery, Beijing Tiantan Hospital, Beijing 100050.
Han
L
L
Wang
S
S
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D002532:Intracranial Aneurysm / Q000201:enzymology* / Q000209:etiology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9