title: Distribution of protein motors along the lateral wall of the outer hair cell.
abstract: The outer hair cell (OHC) plays an important role in the normal functioning of the cochlea, and cochlear amplification is believed to be based on OHC electromotility. This electromotility putatively arises from a conformational change of molecules, i.e., 'protein motors', which would be distributed along the plasma membrane. Although it has been assumed that protein motors are distributed in a restricted area of the plasma membrane, details of such distribution remain unclarified. In this study, first, in order to understand the difference in the stiffness along the cell axis, the local deformation of the OHC in response to hypotonic stimulation is analyzed by measuring the displacement of microspheres attached randomly to the lateral wall of the cell. As a result, the stiffness is expected to be constant throughout the region except in the apical part of the cell, and the stiffness of the apical part is expected to be higher than that of the other regions. Then, the local elongation and contraction of the OHC in response to sinusoidal voltage stimulation are analyzed by measuring the displacement of the microspheres in the same way as in the case of the hypotonic stimulation. From the two measurements mentioned above, it is concluded that there are no motors in the apical and basal parts of the cell, and that the motors are equally distributed along the cell lateral wall in the middle part of the cell.
authors.affiliation: Department of Mechanical Engineering, Tohoku University, Sendai, Japan. wada@cc.mech.tohoku.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Usukura
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: S
authors.initials: S
authors.lastname: Kakehata
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D048429:Cell Size / Q000187:drug effects; D004558:Electric Stimulation; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007038:Hypotonic Solutions / Q000494:pharmacology; D020409:Molecular Motor Proteins / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D007038:Hypotonic Solutions; D020409:Molecular Motor Proteins