A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans.
An isolation procedure comprising affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Toyopearl, and fast protein liquid chromatography on Mono S was used to purify a peptide from broad beans which manifested antifungal activity toward Mycosphaerella arachidicola, Fusarium oxysporum, and Botrytis cinerea. The peptide demonstrated a molecular mass of 7.5 kDa. N-terminal sequence analysis disclosed the identity of the antifungal peptide to be a trypsin-chymotrypsin inhibitor. The trypsin-chymotrypsin inhibitor also exerted an inhibitory action on chymotrypsin activity and HIV-1 reverse transcriptase activity. Proliferation of murine splenocytes was stimulated in the presence of the trypsin-chymotrypsin inhibitor. This report constitutes the first observation of antifungal activity of a leguminous peptidic protease inhibitor.
Ye
X Y
XY
Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
Ng
T B
TB
Rao
P F
PF
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology
D000935:Antifungal Agents; D008934:Mitogens; D018894:Reverse Transcriptase Inhibitors; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors; D054303:HIV Reverse Transcriptase; D002918:Chymotrypsin