Increased generation of superoxide in erythrocytes infected with Babesia gibsoni.
The present study was conducted to clarify the mechanism underlying the oxidative process in erythrocytes infected with Babesia gibsoni. The parasite B. gibsoni was cultured together with erythrocytes from normal dogs for 7 days. When parasitemia reached 12.0-13.4% at Day 7. the production of superoxide in erythrocytes was significantly higher in the parasitized culture than in the control culture (p<0.005). The concentration of thiobarbituric acid reactive substances (TBARS) in erythrocytes in parasitized culture was also significantly increased compared with the control culture (p<0.005), indicating that lipid peroxidation was greater in infected erythrocytes than in non-infected cells. In addition, the rates of superoxide generation in the blood of B. gibsoni-infected dogs were also significantly higher than in non-infected dogs (p<0.001). These results indicate that superoxide anions are increased in erythrocytes parasitized with B. gibsoni. and suggest that oxidative damage, due to lipid peroxidation, might be caused in host erythrocytes by the parasite.
Otsuka
Y
Y
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Yamasaki
M
M
Yamato
O
O
Maede
Y
Y
D000818:Animals; D001403:Babesia / Q000254:growth & development*; D001404:Babesiosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D002374:Catalase / Q000097:blood; D042966:Cytochrome-B(5) Reductase / Q000097:blood; D004283:Dog Diseases / Q000097:blood / Q000201:enzymology / Q000469:parasitology*; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism* / Q000469:parasitology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D016014:Linear Models; D010734:Phosphogluconate Dehydrogenase / Q000097:blood; D017701:Reticulocyte Count / Q000662:veterinary; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides / Q000097:blood*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D042966:Cytochrome-B(5) Reductase; D005980:Glutathione Reductase; D005982:Glutathione Transferase