title: Transcription and RNA replication of tacaribe virus genome and antigenome analogs require N and L proteins: Z protein is an inhibitor of these processes.
abstract: Tacaribe virus (TV), the prototype of the New World group of arenaviruses, comprises a single phylogenetic lineage together with four South American pathogenic producers of hemorrhagic disease. The TV genome consists of two single-stranded RNA segments called S and L. A reconstituted transcription-replication system based on plasmid-supplied TV-like RNAs and TV proteins was established. Plasmid expression was driven by T7 RNA polymerase supplied by a recombinant vaccinia virus. Plasmids were constructed to produce TV S segment analogs containing the negative-sense copy of chloramphenicol acetyltransferase (CAT) flanked at the 5' and 3' termini by sequences corresponding to those of the 5' and 3' noncoding regions of the S genome (minigenome) or the S antigenome (miniantigenome). In cells expressing N and L proteins, input minigenome or miniantigenome produced, respectively, encapsidated miniantigenome or minigenome which in turn produced progeny minigenome or progeny miniantigenome. Both minigenome and miniantigenome in the presence of N and L mediated transcription, which was analyzed as CAT expression. Coexpression of the small RING finger Z (p11) protein was highly inhibitory to both transcription and replication mediated by the minigenome or the miniantigenome. The effect depended on synthesis of Z protein rather than on plasmid or the RNA and was not ascribed to decreased amounts of plasmid-supplied template or proteins (N or L). N and L proteins were sufficient to support full-cycle RNA replication of a plasmid-supplied S genome analog in which CAT replaced the N gene. Replication of this RNA was also inhibited by Z expression.
authors.affiliation: Centro de Virología Animal (CONICET), Serrano 669, C1414DEM Buenos Aires, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: López
authors.forename: R
authors.initials: R
authors.lastname: Jácamo
authors.forename: M T
authors.initials: MT
authors.lastname: Franze-Fernández
mesh_terms: D006477:Arenaviruses, New World / Q000235:genetics*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology*; D019590:Nucleocapsid Proteins / Q000502:physiology*; D010957:Plasmids; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D019590:Nucleocapsid Proteins; D012367:RNA, Viral; D014764:Viral Proteins; C079186:Z protein, Tacaribe virus