Induction of photoreceptor-specific phenotypes in adult mammalian iris tissue.
We show that iris tissue in the adult rat eye, which is embryonically related to the neural retina, can generate cells expressing differentiated neuronal antigens. In addition, the Crx gene transfer induced the specific antigens for rod photoreceptors in the iris-derived cells, which was not seen in the adult hippocampus-derived neural stem cells. Our findings demonstrate a remarkable plasticity of adult iris tissue with potential clinical applications, as autologous iris tissue can be feasibly obtained with peripheral iridectomy.
Haruta
M
M
Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan.
Kosaka
M
M
Kanegae
Y
Y
Saito
I
I
Inoue
T
T
Kageyama
R
R
Nishida
A
A
Honda
Y
Y
Takahashi
M
M
D000375:Aging / Q000235:genetics; D000818:Animals; D000941:Antigens / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D002924:Ciliary Body / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D004627:Embryonic Induction / Q000235:genetics*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005822:Genetic Vectors / Q000502:physiology; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D007498:Iris / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D016900:Neurofilament Proteins / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010786:Photoreceptor Cells / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D051381:Rats; D012243:Rhodopsin / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D000941:Antigens; D018398:Homeodomain Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D016900:Neurofilament Proteins; D015534:Trans-Activators; C109288:cone rod homeobox protein; D016222:Fibroblast Growth Factor 2; C051774:neurofilament protein H; D049452:Green Fluorescent Proteins; D012243:Rhodopsin