The mechanism of glycerol conduction in aquaglyceroporins.
BACKGROUND
The E. coli glycerol facilitator, GlpF, selectively conducts glycerol and water, excluding ions and charged solutes. The detailed mechanism of the glycerol conduction and its relationship to the characteristic secondary structure of aquaporins and to the NPA motifs in the center of the channel are unknown.


RESULTS
Molecular dynamics simulations of GlpF reveal spontaneous glycerol and water conduction driven, on a nanosecond timescale, by thermal fluctuations. The bidirectional conduction, guided and facilitated by the secondary structure, is characterized by breakage and formation of hydrogen bonds for which water and glycerol compete. The conduction involves only very minor changes in the protein structure, and cooperativity between the GlpF monomers is not evident. The two conserved NPA motifs are strictly linked together by several stable hydrogen bonds and their asparagine side chains form hydrogen bonds with the substrates passing the channel in single file.


CONCLUSIONS
A complete conduction of glycerol through the GlpF was deduced from molecular dynamics simulations, and key residues facilitating the conduction were identified. The nonhelical parts of the two half-membrane-spanning segments expose carbonyl groups towards the channel interior, establishing a curve-linear pathway. The conformational stability of the NPA motifs is important in the conduction and critical for selectivity. Water and glycerol compete in a random manner for hydrogen bonding sites in the protein, and their translocations in single file are correlated. The suggested conduction mechanism should apply to the whole family.
Jensen
M O
MO
Beckman Institute, University of Illinois at Urbana-Champaign, 405 N. Mathews, Urbana, IL 61801, USA.
Tajkhorshid
E
E
Schulten
K
K
D020816:Amino Acid Motifs; D020346:Aquaporins / Q000737:chemistry / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D001667:Binding, Competitive; D001692:Biological Transport; D003198:Computer Simulation; D004058:Diffusion; D029968:Escherichia coli Proteins; D005990:Glycerol / Q000378:metabolism*; D006860:Hydrogen Bonding; D008051:Lipid Bilayers / Q000378:metabolism; D008958:Models, Molecular; D009038:Motion; D020836:Protein Structure, Quaternary; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008051:Lipid Bilayers; D014867:Water; C078043:GlpF protein, E coli; D005990:Glycerol