title: Facile synthesis of a fluorescent deoxycytidine analogue suitable for probing the RecA nucleoprotein filament.
abstract: We report the synthesis of the fluorescent 2'-deoxycytidine analogue 5-methylpyrimidin-2-one nucleoside, its incorporation at three specified sites in a single 60-nucleotide DNA molecule, and the use of its total and polarized intrinsic fluorescence to characterize RecA-DNA complexes. [reaction: see text]
authors.affiliation: Department of Chemistry, Rice University, P.O. Box 1892 MS #60, Houston, TX 77251-1892, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Singleton
authors.forename: F
authors.initials: F
authors.lastname: Shan
authors.forename: M W
authors.initials: MW
authors.lastname: Kanan
authors.forename: C M
authors.initials: CM
authors.lastname: McIntosh
authors.forename: C J
authors.initials: CJ
authors.lastname: Stearman
authors.forename: J S
authors.initials: JS
authors.lastname: Helm
authors.forename: K J
authors.initials: KJ
authors.lastname: Webb
mesh_terms: D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D015335:Molecular Probes; D011938:Rec A Recombinases / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes; D003841:Deoxycytidine; D011938:Rec A Recombinases