Cloning, characterization, and tissue expression pattern of mouse Nma/BAMBI during odontogenesis.
Degenerate oligonucleotides to consensus serine kinase functional domains previously identified a novel, partial rabbit tooth cDNA (Zeichner-David et al., 1992) that was used in this study to identify a full-length mouse clone. A 1390-base-pair cDNA clone was isolated encoding a putative 260-amino-acid open reading frame containing a hydrophobic 25-amino-acid potential transmembrane domain. This clone shares some homology with the TGF-beta type I receptor family, but lacks the intracellular kinase domain. DNA database analysis revealed that this clone has 86% identity to a newly isolated human gene termed non-metastatic gene A and 80% identity to a Xenopus cDNA clone termed BMP and activin membrane bound inhibitor. Here we report the mouse Nma/BAMBI cDNA sequence, the tissue expression pattern, and confirmed expression in dental cell lines. This study demonstrates that Nma/BAMBI is a highly conserved protein across species and is expressed at high levels during odontogenesis.
Knight
C
C
University of Texas Health Science Center at San Antonio, Dental School, Department of Pediatric Dentistry, 78229-3900, USA.
Simmons
D
D
Gu
T T
TT
Gluhak-Heinrich
J
J
Pavlin
D
D
Zeichner-David
M
M
MacDougall
M
M
D028341:Activins / Q000037:antagonists & inhibitors; D000596:Amino Acids / Q000032:analysis / Q000235:genetics; D000818:Animals; D020029:Base Pairing / Q000235:genetics; D015152:Blotting, Northern; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D016341:Genes, vif / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009805:Odontogenesis / Q000235:genetics*; D010957:Plasmids; D011817:Rabbits; D016364:Reading Frames / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016212:Transforming Growth Factor beta / Q000235:genetics; D014981:Xenopus; D029867:Xenopus Proteins
D000596:Amino Acids; C485671:BAMBI protein, human; C525819:BMP7 protein, human; C485672:Bambi protein, mouse; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; C121859:bambi-A protein, Xenopus; C525822:bmp7.1 protein, Xenopus; D028341:Activins