Masking of two in vitro immunological assays for Mycobacterium bovis (BCG) in calves acutely infected with non-cytopathic bovine viral diarrhoea virus.
Acute infection of calves, previously vaccinated with bacille Calmette-Guerin (BCG), with non-cytopathic viral diarrhoea virus (BVDV) resulted in the temporary suppression of two in vitro assays used to monitor Mycobacterium bovis infection. Lymphocyte proliferation and interferon-gamma production by whole blood cultures containing purified protein derivatives prepared from Mycobacterium avium (PPD-A) and M bovis (PPD-B) were markedly suppressed. The implication is that acute infections of cattle with non-cytopathic BVDV may temporarily compromise diagnostic tests for M. bovis infections and result in a failure to identify cattle with tuberculosis.
Charleston
B
B
Institute for Animal Health, Compton, Newbury, Berkshire.
Hope
J C
JC
Carr
B V
BV
Howard
C J
CJ
D000208:Acute Disease; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood*; D008213:Lymphocyte Activation / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D009500:Neutralization Tests / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
D007371:Interferon-gamma