title: Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro.
abstract: Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.
authors.affiliation: Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland.
authors.forename: S W
authors.initials: SW
authors.lastname: Kamau
authors.forename: F
authors.initials: F
authors.lastname: Grimm
authors.forename: A B
authors.initials: AB
authors.lastname: Hehl
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D015415:Biomarkers; D003513:Cycloheximide / Q000494:pharmacology; D049452:Green Fluorescent Proteins; D018314:Leishmania infantum / Q000187:drug effects* / Q000254:growth & development; D008164:Luminescent Proteins / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
chemical_list: D000981:Antiprotozoal Agents; D015415:Biomarkers; D008164:Luminescent Proteins; D011500:Protein Synthesis Inhibitors; D049452:Green Fluorescent Proteins; D011691:Puromycin; D000493:Allopurinol; D003513:Cycloheximide