title: Influence of dietary fatty acid, vegetable, and vitamin intake on etheno-DNA adducts in white blood cells of healthy female volunteers: a pilot study.
abstract: Etheno-DNA adducts such as 1,N(6)-ethenodeoxyadenosine (epsilondA) and N(2),3-ethenodeoxycytidine (epsilondC) are formed as result of oxidative stress and lipid peroxidation via reactive alkenals (J. Nair et al., Mutat. Res., 424:59-69, 1999). High pi-6 polyunsaturated fatty acid intake markedly increased levels of WBCs in female volunteers on a controlled diet (J. Nair et al., Cancer Epidemiol. Biomark. Prev., 6:591-601, 1997). In healthy female volunteers we investigated possible correlations between dietary fatty acid intake (by questionnaire), concentration of linoleic acid (LA) and oleic acid (OA) in serum (n = 34), and etheno-DNA adduct levels in WBC (n = 42). Two groups of samples were selected according to dietary intake >15 g (group A) or <5 g (group B) LA/day. Serum samples were analyzed for free OA and LA by gas chromatography-mass spectroscopy and WBC-DNA for epsilondA and epsilondC adducts by immunoaffinity (32)P postlabeling. On a group level, serum LA and OA concentrations were higher in group A than group B, whereas the LA/OA ratios were similar. The mean epsilondA and epsilondC levels did not significantly differ in groups A and B, but a third of the individuals had more than twice the mean adduct levels than the rest. Correlation analyses revealed a significant inverse correlation for epsilondA in WBC-DNA and vegetable or vitamin E consumption. We conclude that etheno-DNA adduct levels are not determined by LA intake alone but might depend on the ratio of pi-6 polyunsaturated fatty acid:other fatty acids and of antioxidants consumed in the diet. This pilot study also indicated a protective effect of dietary vitamin E and vegetables against miscoding, lipid peroxidation-induced DNA lesions.
authors.affiliation: Division of Toxicology, German Cancer Research Center (Deutsches Krebsforschungszentrum), 69120 Heidelberg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Hagenlocher
authors.forename: J
authors.initials: J
authors.lastname: Nair
authors.forename: N
authors.initials: N
authors.lastname: Becker
authors.forename: A
authors.initials: A
authors.lastname: Korfmann
authors.forename: H
authors.initials: H
authors.lastname: Bartsch
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D018736:DNA Adducts / Q000097:blood*; D003839:Deoxyadenosines / Q000378:metabolism; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000378:metabolism; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation; D008875:Middle Aged; D019301:Oleic Acid / Q000378:metabolism; D018384:Oxidative Stress; D010865:Pilot Projects; D014675:Vegetables / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018736:DNA Adducts; D003839:Deoxyadenosines; D005227:Fatty Acids; D014815:Vitamins; D019207:beta Carotene; D003841:Deoxycytidine; D014810:Vitamin E; D019301:Oleic Acid; C032221:1,N(6)-ethenodeoxyadenosine; D019787:Linoleic Acid