[Studies of the determination of glycyrrhizin acid kali salt by HPLC and the pharmacokinetics].
To find a practive and quick method determining GK concentration in blood by HPLC and to study its pharmacokinetics. GK from Glycyrrhiza uralensis Fisch was extracted and purified. GK were administered to mice by i.v. and ig. The Concentration of GK in blood at expected time was determined by HPLC. The data was dealed with by 3 P 87 programe. The concentration-time curve of i.v. 0.1% GK 40 mg/kg is in accordance with tow-compartment model. t1/2 alpha = 5.128 min, t1/2 beta = 51.196 min, AUC = 7477.642 (micrograms/ml).min, Cl(s) = 0.0956 microgram/min(microgram/ml). The c-t curve of ig. 2.2% GK 500 mg/kg is in accordance with tow compartment model, t1/2 alpha = 15.97 min, t1/2 beta = 200.05 min, AUC = 2453.96(micrograms/ml).min, Cl(s) = 3.642 micrograms/min(micrograms/ml). HPLC is a quick and practive method to determin the concentration of GK in blood.
Shao
W
W
College of Pharmacy, Shandong University, Jinan 250012.
Zhang
L
L
Wang
C
C
Mi
G
G
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000097:blood / Q000493:pharmacokinetics*; D051379:Mice
D019695:Glycyrrhizic Acid