title: [Heparin enhances the stimulating effect of thrombopoietin on megakaryocytopoiesis].
abstract: OBJECTIVE
  To verify whether heparin could enhance the stimulating effect of Tpo on megakaryocytopoiesis.
  
  
  METHODS
  Megakaryocytic leukemia cell line M07e, human cord blood CD34+ cells and BALB/c mice were used in this study. The effects of heparin and/or Tpo on megakaryocytopoiesis were studied by [3H]-TdR incorporation assay, CFU-MK plasmic semi-solid culture and in vivo experiment in BALB/c mice.
  
  
  RESULTS
  Heparin could enhance the stimulating effect of Tpo on stimulating the proliferation of M-07e, the growth of CFU-MK from human cord blood CD34+ cells and in vivo megakaryocytopoiesis in mice.
  
  
  CONCLUSIONS
  Heparin participates in positive regulation of megakaryocytopoiesis by enhancing the megakaryocytopoietic activities of Tpo.
authors.affiliation: Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Shen
authors.forename: Z
authors.initials: Z
authors.lastname: Han
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013926:Thrombopoietin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D006493:Heparin; D013926:Thrombopoietin