In vitro evaluation of secoiridoid glucosides from the fruits of Ligustrum lucidum as antiviral agents.
Six secoiridoid glucosides, lucidumoside C (1), oleoside dimethylester (2), neonuezhenide (3), oleuropein (4), ligustroside (5) and lucidumoside A (6), isolated from the fruits of Ligustrum lucidum (Oleaceae), were examined in vitro for their activities against four strains of pathogenic viruses, namely herpes simplex type I virus (HSV-1), influenza type A virus (Flu A), respiratory syncytial virus (RSV) and parainfluenza type 3 virus (Para 3). Antiviral activities were evaluated by the cytopathic effect (CPE) inhibitory assay. The purpose was to check if the antioxidative potency of these glucosides correlated with their antiviral potency. Results showed that none of the glucosides had any significant activity against HSV-1 and Flu A. Oleuropein, however, showed significant antiviral activities against RSV and Para 3 with IC50 value of 23.4 and 11.7 microg/ml, respectively. Lucidumoside C, oleoside dimethylester and ligustroside showed potent or moderate antiviral activities against Para 3 with IC50 values of 15.6-20.8 microg/ml. These results also documented that the anti-oxidative potency of these secoiriodoid glucosides was not directly related to their antiviral effects.
Ma
S C
SC
Department of Biology and Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin.
He
Z D
ZD
Deng
X L
XL
But
P P
PP
Ooi
V E
VE
Xu
H X
HX
Lee
S H
SH
Lee
S F
SF
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D005638:Fruit / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects; D039823:Iridoids; D029592:Oleaceae / Q000737:chemistry*; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018113:Respiratory Syncytial Virus, Human / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014709:Vero Cells / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D000998:Antiviral Agents; D005960:Glucosides; D039823:Iridoids; D011714:Pyrans