[Development of donor cells in chicken-duck chimeric embryos].
Chicken W chromosome-specific DNA fragments were obtained after transformation, extraction, purification and digestion with EcoR I of pUGD1201. The W chromosome-specific DNA probe was a 1.2 kb insert, which was labelled by the random-technique using a Digoxigenin Labeling Kit. Chicken PGCs from a line of White Leghorns were microinjected into Maya duck blastoderms at stage X. The embryos at 6,8,9,12,16,18 days of incubation were used to study the development of chicken PGCs in the chimeric embryos. Used chicken W chromosome-specific DNA probes by in situ hybridization, development of donor cells in chicken-duck chimeric embryos were studied. In 54 chimeric embryos, there were different degrees of chimerism in the organs. The chimeric rate of the livers was the highest, which of the gonads was the lowest. It is demonstrated that somatic and germline chimeras can be yielded successfully by transferring stage X chicken blastodermal cells.
Ma
Y Z
YZ
College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China.
Li
Z D
ZD
Sha
J
J
Liu
C H
CH
Wang
N
N
D000818:Animals; D002455:Cell Division; D002645:Chickens; D002678:Chimera; D015342:DNA Probes; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000166:cytology*; D017403:In Situ Hybridization; D013234:Stem Cells / Q000502:physiology*
D015342:DNA Probes