title: Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.
abstract: Kinetoplastid protozoa regulate their gene expression primarily through control of mRNA degradation and translation. We describe here the degradation of three reporter mRNAs in Trypanosoma brucei. One mRNA had the 3'-untranslated region (3'-UTR) from the developmentally regulated EP1 mRNA, which is abundant in the procyclic (tsetse fly) form of the parasite but is almost undetectable in the bloodstream form. This untranslated region includes a 26 nt U-rich sequence that causes extreme RNA instability in the bloodstream form. The two other RNAs, which are not developmentally regulated, had either the actin 3'-UTR, or a version of the EP1 sequence lacking the 26 nt bloodstream-form instability element. All RNAs had poly(A) tails approximately 200 nt long, in both bloodstream and procyclic forms. Degradation of the two constitutively expressed mRNAs involved deadenylation and degradation by both 5'-->3' and 3'-->5' exonucleases. In contrast, in bloodstream forms, the 3'-end of the RNA bearing the bloodstream-form instability element disappeared very rapidly after transcription inhibition and partially deadenylated intermediates were not seen. The instability element may cause extremely rapid deadenylation, or it may be targeted by an endonuclease.
authors.affiliation: Zentrum für Molekulare Biologie Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Irmer
authors.forename: C
authors.initials: C
authors.lastname: Clayton
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins
chemical_list: D020413:3' Untranslated Regions; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C053277:procyclic acidic repetitive protein, Trypanosoma; D011061:Poly A; D015500:Chloramphenicol O-Acetyltransferase; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases