Maintenance of glycoprotein-determined phenotype in an HIV type 1 (pNL43) env gene-cassetting system.
Here we report the construction and use of a full-length env gene-cassetting system, C2, based on the HIV-1 infectious molecular clone NL43. C2 produces virus with the same phenotype as NL43 but with 2-fold lower growth kinetics. The latter probably relates to alteration in the vpu and/or nef genes. C2-env chimeras of macrophage-tropic and T cell-tropic laboratory strains and primary HIV-1 isolates retain the glycoprotein-determined phenotypes of their parent viruses. The cassette will assist studies of HIV-1 pathogenesis.
Zheng
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Virology Division, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Daniels
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RS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D015686:Gene Products, env / Q000235:genetics* / Q000502:physiology; D015752:Genes, env / Q000235:genetics* / Q000502:physiology; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA
D015686:Gene Products, env; D011993:Recombinant Fusion Proteins