Metabolism of NAD+ in nuclei of Saccharomyces cerevisiae during stimulation of its biosynthesis by nicotinamide.
The activities of nuclear enzymes involved in NAD+ metabolism in Saccharomyces cerevisiae strain 913a-1 and its mutant 110 previously selected as an NAD+ producer were investigated. The presence of extracellular nicotinamide increased the total NAD+ pool in the cells and increased [3H]nicotinic acid incorporation; however, NAD+ concentration in isolated nuclei decreased slightly. The stimulating effect of nicotinamide on intracellular synthesis of NAD+ correlated with increases in ADP-ribosyl transferase, NAD+-pyrophosphorylase, and NAD+ase activities.
Gulyamova
T G
TG
Institute of Microbiology, Uzbekistan Academy of Sciences, ul. Abdully Kodiry 7B, Tashkent, 700128 Uzbekistan. imbasru@uzsci.net
Ruzieva
D M
DM
Nasmetova
S M
SM
Shakirzyanova
M R
MR
Kerbalaeva
A M
AM
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009536:Niacinamide / Q000378:metabolism* / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
D009243:NAD; D000246:Adenosine Diphosphate Ribose; D009536:Niacinamide; D011065:Poly(ADP-ribose) Polymerases; D011755:Pyrophosphatases; C024289:NAD pyrophosphatase