Transient dissociation of polyribosomes and concurrent recruitment of calreticulin and calmodulin transcripts in gravistimulated maize pulvini.
The dynamics of polyribosome abundance were studied in gravistimulated maize (Zea mays) stem pulvini. During the initial 15 min of gravistimulation, the amount of large polyribosomes transiently decreased. The transient decrease in polyribosome levels was accompanied by a transient decrease in polyribosome-associated mRNA. After 30 min of gravistimulation, the levels of polyribosomes and the amount of polyribosome-associated mRNA gradually increased over 24 h up to 3- to 4-fold of the initial value. Within 15 min of gravistimulation, total levels of transcripts coding for calreticulin and calmodulin were elevated 5-fold in maize pulvinus total RNA. Transcripts coding for calreticulin and calmodulin were recruited into polyribosomes within 15 min of gravistimulation. Over 4 h of gravistimulation, a gradual increase in the association of calreticulin and calmodulin transcripts with polyribosomes was seen predominantly in the lower one-half of the maize pulvinus; the association of transcripts for vacuolar invertase with polyribosomes did not change over this period. Our results suggest that within 15 min of gravistimulation, the translation of the majority of transcripts associated with polyribosomes decreased, resembling a general stress response. Recruitment of calreticulin and calmodulin transcripts into polyribosomes occurred predominantly in the lower pulvinus one-half during the first 4 h when the presentation time for gravistimulation in the maize pulvinus is not yet complete.
Heilmann
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I
Department of Botany, North Carolina State University, Raleigh, NC 27695-7612, USA. heilmann@bnl.gov
Shin
J
J
Huang
J
J
Perera
I Y
IY
Davies
E
E
D001692:Biological Transport; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D037282:Calreticulin; D006026:Glycoside Hydrolases / Q000378:metabolism; D018522:Gravitropism; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D018516:Pulvinus / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D018749:RNA, Plant; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic; D014617:Vacuoles / Q000378:metabolism; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*; D043324:beta-Fructofuranosidase
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D037282:Calreticulin; D012333:RNA, Messenger; D018749:RNA, Plant; D012261:Ribonucleoproteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase