Immunohistochemical localization of activated and mature CD83+ dendritic cells in granulomas of sporotrichosis.
Dendritic cells (DC) are considered to be the most potent antigen-presenting cells (APC) and CD83 is expressed at a high level on immune-competent, activated and mature DC. In order to obtain insight into the immune response in sporotrichosis, we searched immunohistochemically for CD83+ DC in the granulomatous skin lesions of patients. A small but significant subpopulation of CD83+ DC (approximately 0.2 to 0.8% of inflammatory cells) was found. The most common distribution pattern of CD83+ DC was as clusters with mononuclear lymphoid cells within the granulomas. In addition, CD83+ DC were occasionally scattered around the granulomas. High-intensity staining of CD83 antigens was detected not only on the surface, but also in the cytoplasm of DC. No CD83+ DC were observed in the epidermis. A considerable amount of CD1a+ cells were detected in the epidermis, papillary dermis and around the granulomas, but CD1a+ cells were rarely found within the granulomas. There were large numbers of CD68+ monocytes and macrophages in the granulomas. These results indicate that activated and mature DC may play a role in the immune response to sporotrichosis.
Koga
T
T
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, J-812-8582 Fukuoka, Japan. tekoga@dermatol.med.kyushu-u.ac.jp
Duan
H
H
Urabe
K
K
Furue
M
M
D015703:Antigens, CD; D001706:Biopsy / Q000379:methods; D003713:Dendritic Cells / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D006099:Granuloma / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis*; D012867:Skin / Q000276:immunology; D013174:Sporotrichosis / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D000097802:CD83 Antigen
D015703:Antigens, CD; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen