title: DNA damage and apoptosis in the immature mouse cerebellum after acute exposure to a 1 mT, 60 Hz magnetic field.
abstract: Several recent studies have reported that whole-body exposure of rodents to power frequency magnetic fields (MFs) can result in DNA single- and double-strand breaks in the brains of these animals. The current study was undertaken to investigate whether an acute 2h exposure of a 1 mT, 60 Hz MF could elicit DNA damage, and subsequently apoptosis, in the brains of immature (10-day-old) mice. DNA damage was quantitated at 0, 2, 4, and 24h after exposure using the alkaline comet assay. Apoptosis was quantitated in the external granule cell layer (EGCL) of the immature mouse cerebellum at 0 and 24h after exposure to MF by the TdT-mediated dUTP nick-end labeling (TUNEL) assay. Four parameters (tail ratio, tail moment, comet length and tail length) were used to assess DNA damage for each comet. While increased DNA damage was detected by tail ratio at 2h after MF exposure, no supporting evidence of increased DNA damage was detected by the other parameters. In addition, no similar differences were observed using these parameters at any of the other post-exposure times. No increase in apoptosis was observed in the EGCL of MF-exposed mice, when compared to sham mice. Taken together, these results do not support the hypothesis that acute MF exposure causes DNA damage in the cerebellums of immature mice.
authors.affiliation: Consumer and Clinical Radiation Protection Bureau, Product Safety Programme, Health Canada, 775 Brookfield Rd., Ottawa, Ont., Canada K1A 1C1. james_mcnamee@hc-sc.gc.ca
authors.forename: J P
authors.initials: JP
authors.lastname: McNamee
authors.forename: P V
authors.initials: PV
authors.lastname: Bellier
authors.forename: J R N
authors.initials: JR
authors.lastname: McLean
authors.forename: L
authors.initials: L
authors.lastname: Marro
authors.forename: G B
authors.initials: GB
authors.lastname: Gajda
authors.forename: A
authors.initials: A
authors.lastname: Thansandote
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D002531:Cerebellum / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D005260:Female; D008280:Magnetics; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy
chemical_list: D004247:DNA