The Sec34/35 Golgi transport complex is related to the exocyst, defining a family of complexes involved in multiple steps of membrane traffic.
The specificity of intracellular vesicle transport is mediated in part by tethering factors that attach the vesicle to the destination organelle prior to fusion. We have identified a protein, Dor1p, that is involved in vesicle targeting to the yeast Golgi apparatus and found it to be associated with seven further proteins. Identification of these revealed that they include Sec34p and Sec35p, the two known components of the Sec34/35 complex previously proposed to tether vesicles to the Golgi. Of the six previously uncharacterized components, four have homologs in higher eukaryotes, including a subunit of a mammalian Golgi transport complex. Furthermore, several of the proteins show distant homology to components of two other putative tethering complexes, the exocyst and the Vps52/53/54 complex, revealing that tethering factors involved in different membrane traffic steps are structurally related.
Whyte
J R
JR
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Munro
S
S
D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000502:physiology; D010641:Phenotype; D021381:Protein Transport / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins; D015003:Yeasts
D033942:Adaptor Proteins, Vesicular Transport; C112749:COG2 protein, S cerevisiae; C401396:COG3 protein, S cerevisiae; C498528:COG3 protein, human; C439910:COG8 protein, S cerevisiae; D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C093602:VPS52 protein, S cerevisiae; C492177:VPS53 protein, S cerevisiae; C117606:VPS54 protein, S cerevisiae; D033921:Vesicular Transport Proteins