Malignant transformation of primary chicken spleen cells by human transcription factor c-Rel.
Rel/NF-kappaB transcription factors control a variety of cellular processes, such as cell growth and apoptosis, that are relevant to oncogenesis, and mutations in genes encoding Rel/NF-kappaB transcription factors have been found in several human lymphoid cell cancers. In this study, we have used a sensitive cell outgrowth assay to demonstrate that wild-type human c-Rel can malignantly transform primary chicken spleen cells, and that transformation by c-Rel is accelerated by co-expression of Bc1-2. Full-length mouse c-Rel can also transform chicken spleen cells. These results are the first demonstration of a lymphoid cell malignant transforming ability for mammalian Rel/NF-kappaB transcription factors, and implicate c-Rel as a molecular target for cancer therapeutics.
Gilmore
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Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts, MA 02215, USA. gilmore@bu.edu
Cormier
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Jean-Jacques
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Gapuzan
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D000362:Agar; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002645:Chickens; D003114:Colony-Forming Units Assay / Q000379:methods; D003470:Culture Media; D015972:Gene Expression Regulation, Neoplastic; D019254:Genes, bcl-2; D020573:Genes, rel; D006801:Humans; D051379:Mice; D016328:NF-kappa B / Q000502:physiology; D020572:Oncogene Proteins v-rel / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000502:physiology; D020569:Proto-Oncogene Proteins c-rel / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015398:Signal Transduction; D013045:Species Specificity; D013154:Spleen / Q000166:cytology*; D014158:Transcription, Genetic; D014162:Transfection; D051020:bcl-X Protein
C494811:BCL2L1 protein, human; C494812:Bcl2l1 protein, mouse; D003470:Culture Media; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D019253:Proto-Oncogene Proteins c-bcl-2; D020569:Proto-Oncogene Proteins c-rel; D011993:Recombinant Fusion Proteins; D051020:bcl-X Protein; D000362:Agar