Cloning and characterization of a novel gene encoding keratin-like protein from nematode Nippostrongylus brasiliensis.
Nippostrongylus brasiliensis (Nb) is one of the most important parasites in studying Th2 immune response of the host, but little is known about its antigenic structures of the excretory-secretory or structural proteins of the parasite. Here we report cloning and characterization of a novel antigenic gene from cDNA library of Nb adult worm by immunoscreening. The positive clone, KLP-Nb, had an open reading frame of 612 bp that encodes a 203-amino-acid protein and was homologous to 'similar to keratins in a glycine-rich region' of Caenorhabditis elegans. Its expression was confirmed by Northern blotting and IgG enzyme-linked immunosorbent assay. This protein seems to be one of the components of cuticle that covers the nematode body.
Shibui
A
A
Department ofPediatrics, Shinshu University School of Medicine, Asahi, Matsumoto, Japan. shibui@sch.md.shinshu-u.ac.jp
Takamoto
M
M
Shi
Y
Y
Komiyama
A
A
Sugane
K
K
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D007633:Keratins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009559:Nippostrongylus / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D016415:Sequence Alignment
D007633:Keratins