Rapid detection of several mycobacterial species using a polymerase chain reaction reverse hybridisation assay.
The ability of a polymerase-chain-reaction-based technique combined with a reverse hybridisation line-probe assay to detect and identify eight of the most clinically significant mycobacterial species directly from cultures in liquid medium was evaluated. The line-probe assay allows simultaneous identification of eight different mycobacterial species. Ninety-seven mycobacterial strains belonging to 22 different species were evaluated. All strains were previously inoculated into MB/BacT bottles (Organon Teknika, USA). The sensitivity and specificity of the test when applied on positive MB/BacT liquid cultures containing isolates from mycobacterial species included as specific probes on the line-probe assay strip was 100% and 100%, respectively. These results further support the potential clinical usefulness of this technique in the diagnosis of mycobacterial infections.
Padilla
E
E
Hospital Universitario Germans Trias i Pujol, Departamento de Microbiología y Genética, Universidad Autónoma de Barcelona, Badalona, Spain. epadilla@ns.hugtip.scs.es
Manterola
J M
JM
González
V
V
Lonca
J
J
Domínguez
J
J
Matas
L
L
Galí
N
N
Ausina
V
V
D015373:Bacterial Typing Techniques / Q000379:methods; D003470:Culture Media; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D003470:Culture Media