Novel spermine-amino acid conjugates and basic tripeptides enhance cleavage of the hairpin ribozyme at low magnesium ion concentration.
Combinations of the polyamine spermine and magnesium ions synergize to dramatically enhance cleavage of the hairpin ribozyme. Certain synthetic basic tripeptides stimulate hairpin cleavage significantly at limiting magnesium ion concentration, notably the tripeptide of L-diaminobutyric acid (Dab). Of a range of novel synthetic spermine-amino acid conjugates, L-Dab-spermine (but not D-Dab nor other amino acid conjugates) was more effective than spermine itself.
Stolze
K
K
Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Holmes
S C
SC
Earnshaw
D J
DJ
Singh
M
M
Stetsenko
D
D
Williams
D
D
Gait
M J
MJ
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D000613:Aminobutyrates / Q000737:chemistry; D001671:Biochemistry / Q000379:methods; D008274:Magnesium / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D013096:Spermine / Q000737:chemistry* / Q000378:metabolism
D000596:Amino Acids; D000613:Aminobutyrates; D010455:Peptides; D016337:RNA, Catalytic; D013096:Spermine; C005959:2,4-diaminobutyric acid; D008274:Magnesium