Solid state NMR for determination of degree of acetylation of chitin and chitosan.
The degree of acetylation (DA) of various samples has been evaluated by 1H liquid-state NMR and 13C and 15N CP-MAS solid-state NMR over the whole range of DA. A good agreement has been found for all the experiments. The 13C and 15N CP-MAS experiments have permitted the evaluation of the chitin DA and content in the structural polysaccharides in a fungus named Aspergillus niger. The fungus structural carbohydrates mainly consist in pure chitin associated with glucans. Comparison of the 13C CP-MAS spectra with standard (1-->3)-beta-D-glucans strongly suggests that chitin and glucans are linked via covalent bonds.
Heux
L
L
Centre de Recherches sur les Macromolécules Végétales (CNRS), Joseph Fourier University, BP 53, 38041 Grenoble, France.
Brugnerotto
J
J
Desbrières
J
J
Versali
M F
MF
Rinaudo
M
M
D000107:Acetylation; D001234:Aspergillus niger / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry*; D048271:Chitosan; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry
D011134:Polysaccharides; D002686:Chitin; D048271:Chitosan