Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis.
A cDNA expression library prepared from mRNA of Haemaphysalis longicornis (H. longicornis) was screened with a H. longicornis-infested rabbit serum. A cDNA encoding 27/30kDa proteins was cloned and designated P27/30 gene. The predicted amino acid sequence of the P27/30 gene shows a rather high homology (58% amino acid identities and 11% amino acid similarity) with Drosophila melanogaster troponin I clone E2. H. longicornis P27/30 possesses amino acid sequence of actin-binding domains of troponin I at the amino acid residues 128-148, suggesting that H. longicornis P27/30 is a troponin I-like protein. By immunoblot analysis, mouse anti-recombinant P27/30 serum reacted with major constituent protein bands in extracts of adult ticks, and also immunoreacted with muscle, cuticle, gut, and salivary gland in H. longicornis ticks. Moreover, immunohistochemistry using the anti-P27/30 serum showed a strong reactivity in muscle, suggesting that native P27/30 is expressed abundantly in that tissue.
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Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, 501-1193, Gifu, Japan.
Xuan
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X
Tsuji
N
N
Kamio
T
T
Igarashi
I
I
Nagasawa
H
H
Mikami
T
T
Fujisaki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004195:Disease Models, Animal; D004331:Drosophila melanogaster; D004926:Escherichia coli; D015870:Gene Expression; D015723:Gene Library; D005820:Genetic Testing; D026863:Ixodidae / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000469:parasitology*; D019210:Troponin I / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D008840:Microfilament Proteins; C444437:P27-30 protein, Haemaphysalis longicornis; D019210:Troponin I