Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit.
A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.
Jaakola
L
L
Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland. Laura.Jaakola@oulu.fi
Pirttilä
A M
AM
Halonen
M
M
Hohtola
A
A
D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D008623:Mercaptoethanol / Q000494:pharmacology; D008967:Molecular Biology / Q000379:methods; D010952:Plasma Substitutes / Q000494:pharmacology; D011205:Povidone / Q000494:pharmacology; D012313:RNA / Q000032:analysis / Q000302:isolation & purification*; D029796:Vaccinium / Q000378:metabolism*
D002593:Cetrimonium Compounds; D018076:DNA, Complementary; D010952:Plasma Substitutes; D008623:Mercaptoethanol; D012313:RNA; D011205:Povidone; D000077286:Cetrimonium