A population study of the normal cornea using an in vivo, slit-scanning confocal microscope.
OBJECTIVE
To document qualitative and quantitative changes in the normal, healthy human cornea with age using the confocal microscope.


METHODS
The central corneas of 120 subjects (mean age, 41 years; range, 11 to 80 years) were examined using an in vivo slit-scanning real-time confocal microscope. Images of the corneal stroma and endothelium from both eyes of each subject were semiautomatically analyzed in an observer-masked, randomized manner.


RESULTS
Anterior keratocyte density, posterior keratocyte density, and endothelial cell density were shown to be unaffected by the sex of the subject with p values of 0.46, 0.55, 0.50, respectively (multivariate analysis of variance). No statistically significant difference was detected between right and left eyes for all corneal layers examined. The anterior keratocyte density, posterior keratocyte density, and endothelial cell density decreased at a rate of 0.48, 0.22, and 0.33% per year, respectively. A positive correlation was found between the coefficient of cell variation and age.


CONCLUSIONS
This data constitutes essential normative data that can be used as a control in further research into abnormal corneal conditions.
Hollingsworth
J
J
Eurolens Research, Dept of Optometry and Neuroscience, UMIST, Manchester, United Kingdom. jo.hollingsworth@stud.umist.ac.uk
Perez-Gomez
I
I
Mutalib
H A
HA
Efron
N
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D002452:Cell Count; D002648:Child; D003319:Corneal Stroma / Q000166:cytology*; D004311:Double-Blind Method; D004728:Endothelium, Corneal / Q000166:cytology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged