Facile synthesis of a fluorescent deoxycytidine analogue suitable for probing the RecA nucleoprotein filament.
We report the synthesis of the fluorescent 2'-deoxycytidine analogue 5-methylpyrimidin-2-one nucleoside, its incorporation at three specified sites in a single 60-nucleotide DNA molecule, and the use of its total and polarized intrinsic fluorescence to characterize RecA-DNA complexes. [reaction: see text]
Singleton
S F
SF
Department of Chemistry, Rice University, P.O. Box 1892 MS #60, Houston, TX 77251-1892, USA.
Shan
F
F
Kanan
M W
MW
McIntosh
C M
CM
Stearman
C J
CJ
Helm
J S
JS
Webb
K J
KJ
D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D015335:Molecular Probes; D011938:Rec A Recombinases / Q000737:chemistry*
D005456:Fluorescent Dyes; D015335:Molecular Probes; D003841:Deoxycytidine; D011938:Rec A Recombinases