Reduced hyperalgesia induced by nerve injury, but not by inflammation in mice lacking protein kinase C gamma isoform.
Protein kinase C is one of protein kinases which might be involved in the nerve injury- or inflammation-induced hyperalgesia. The present study was designed to investigate the hyperalgesia with thermal paw-withdrawal test induced by sciatic nerve ligation or by intraplantar injection of a complete Freund's adjuvant solution in protein kinase C gamma knockout and its wild-type mice. Either sciatic nerve ligation or intraplantar injection of a complete Freund's adjuvant caused a marked decrease of the paw-withdrawal latency only on the ipsilateral, but not on the contralateral side of the paw in wild-type mice. This ipsilateral hyperalgesia induced by sciatic nerve ligation was significantly attenuated in protein kinase C gamma knockout mice. On the other hand, the ipsilateral hyperalgesia induced by complete Freund's adjuvant remained about the same in protein kinase C gamma knockout mice as in wild-type mice. The results indicate that protein kinase C gamma is involved in the development of the thermal hyperalgesia induced by nerve ligation, but not by complete Freund's adjuvant-induced inflammation.
Ohsawa
M
M
Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Narita
M
M
Mizoguchi
H
H
Cheng
E
E
Tseng
L F
LF
D000818:Animals; D004195:Disease Models, Animal; D005620:Freund's Adjuvant; D006930:Hyperalgesia / Q000201:enzymology* / Q000235:genetics / Q000517:prevention & control*; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics; D007527:Isoenzymes / Q000172:deficiency* / Q000235:genetics*; D008026:Ligation; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011493:Protein Kinase C / Q000172:deficiency* / Q000235:genetics*; D012584:Sciatic Nerve / Q000201:enzymology* / Q000473:pathology* / Q000503:physiopathology
D007527:Isoenzymes; D005620:Freund's Adjuvant; C087857:protein kinase C gamma; D011493:Protein Kinase C