[A comparison of serological and high resolution DNA methods for HLA-B40 cross-reactive groups typing].
OBJECTIVE
To compare the high-resolution DNA typing method with serological method for HLA-B40 cross-reactive groups (CREG) typing in Chinese population.


METHODS
A total of 199 consecutive samples were entered into the study, including 177 identified serologically as B40 CREG positive, 12 ambiguous and 10 negative controls. Double-blind typing for HLA-B40 CREG was carried out using PCR-SSP high-resolution DNA typing and serological one-step monoclonal antibody technique, respectively.


RESULTS
In 3 of the samples serological typing could not be performed owing to poor cell viability, while in 1 PCR-SSP typing could not be done owing to poor DNA. Among samples that were successfully typed by both methods, all alleles of HLA-B40 CREG could be accurately distinguished by PCR-SSP. It was proved that PCR-SSP was a high-sensitivity and high-specificity technique. However, serology showed 11.2% misassignments. The overall time for serological typing was 2 hours, and for DNA typing, 5 hours. The percentages of HLA-B40 CREG alleles in Chinese population were 67.4% for B60, 20.8% for B61, 9.6% for B4801 and 2.2% for B4005.


CONCLUSIONS
High resolution PCR-SSP typing for HLA-B40 CREG could be used in routine clinical practice with a greater precision than serology.
Tan
J
J
Renal Transplant Center, Shanghai First People's Hospital, Shanghai 350025.
Tang
X
X
Gu
W
W
D003429:Cross Reactions; D004247:DNA / Q000032:analysis*; D015235:HLA-B Antigens / Q000276:immunology*; D059947:HLA-B40 Antigen; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods; D012698:Serologic Tests / Q000379:methods; D016037:Single-Blind Method
D015235:HLA-B Antigens; D059947:HLA-B40 Antigen; D004247:DNA