title: Cloning and characterization of a metal responsive element-containing fragment from the Wilson disease gene locus by junction trapping.
abstract: All mammalian metallothionein genes studied to date have several metal responsive elements (MRE) with consensus sequences of TGCRCNC (R, purine) in their regulatory region. MRE-like sequences were also found in many other metal-related genes. To see whether there is also such a sequence at the genetic locus (13q14.3) of Wilson disease, which is a genetic disorder of copper metabolism, junction-trapping method based on the MRE sequence was used. A fragment containing MRE and MRE-like sequences from YAC 27D8 at the WND locus was successfully cloned and mapped back to the YAC by PCR. Presence of such a sequence in the copper transporter gene at the WD locus might imply that it has a possible interesting role in the regulation of WD gene expression.
authors.affiliation: Department of Medical Genetics, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005.
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: H Y
authors.initials: HY
authors.lastname: LoWilson
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003300:Copper; D000073840:Copper-Transporting ATPases; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins; D006527:Hepatolenticular Degeneration / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D000097809:Transcription Factor MTF-1
chemical_list: D000251:Adenosine Triphosphatases; D027682:Cation Transport Proteins; D003300:Copper; D000073840:Copper-Transporting ATPases; D004247:DNA; D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1