title: Differentially expressed genes in rat dorsal root ganglia following peripheral nerve injury.
abstract: Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein 1B, protein tyrosine phosphatase alpha, Kv1.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain.
authors.affiliation: Department of Anatomy, School of Medicine, Kyungpook National University, 2-101, Dongin Dong, Taegu, 700-422, Korea.
authors.forename: D S
authors.initials: DS
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: S Y
authors.initials: SY
authors.lastname: Park
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoo
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: K J
authors.initials: KJ
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Cho
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000235:genetics; D053399:Apolipoproteins D; D001483:Base Sequence / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005727:Ganglia, Spinal / Q000293:injuries / Q000378:metabolism* / Q000503:physiopathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D051663:Kv1.2 Potassium Channel; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009437:Neuralgia / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D016900:Neurofilament Proteins / Q000235:genetics; D010523:Peripheral Nervous System Diseases / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000235:genetics; D024642:Potassium Channels, Voltage-Gated; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001053:Apolipoproteins; D053399:Apolipoproteins D; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; C496972:Kcna2 protein, rat; D051663:Kv1.2 Potassium Channel; D008869:Microtubule-Associated Proteins; D009185:Myelin Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C489545:Pmp22 protein, mouse; C489546:Pmp22 protein, rat; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; C085284:microtubule-associated protein 1B; C061195:neurofilament protein M; D017027:Protein Tyrosine Phosphatases