Cloning of precursors for two MIH/VIH-related peptides in the prawn, Macrobrachium rosenbergii.
Two cDNA clones (634 and 1366 bp) encoding MIH/VIH (molt-inhibiting hormone/vitellogenesis-inhibiting hormone)-related peptides were isolated and sequenced from a Macrobrachium rosenbergii eyestalk ganglia cDNA library. The clones contain a 360 and 339 bp open-reading frame, and their conceptually translated peptides consist of a 41 and 34 amino acid signal peptide, respectively, and a 78 amino acid residue mature peptide hormone. The amino acid sequences of the peptides exhibit higher identities with other known MIHs and VIH (44-69%) than with CHHs (28-33%). This is the first report describing the cloning and sequencing of two MIH/VIH-related peptides in a single crustacean species. Transcription of these mRNAs was detected in the eyestalk ganglia, but not in the thoracic ganglia, hepatopancreas, gut, gill, heart, or muscle.
Yang
W J
WJ
Center for Environmental Diagnostics and Bioremediation, University of West Florida, 11000 University Parkway, Pensacola, Florida 32514-5751, USA.
Rao
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D015723:Gene Library; D007447:Invertebrate Hormones; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D033541:Palaemonidae; D010455:Peptides / Q000737:chemistry; D011061:Poly A; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014158:Transcription, Genetic
D002352:Carrier Proteins; D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; C101529:molt-inhibiting hormone, crayfish; C073189:vitellogenesis inhibiting hormone; D011061:Poly A