title: Identification and expression of cephamycinase bla(CMY) genes in Escherichia coli and Salmonella isolates from food animals and ground meat.
abstract: Twenty-one Salmonella and 54 Escherichia coli isolates, recovered from food animals and retail ground meats, that exhibited decreased susceptibilities to ceftiofur and ceftriaxone were shown to possess a bla(CMY) gene. The bla(CMY-4) gene was identified in an E. coli isolate recovered from retail chicken and was further shown to be responsible for resistance to cephalothin, ampicillin, and amoxicillin-clavulanic acid and elevated MICs of ceftriaxone, cefoxitin, and ceftiofur.
authors.affiliation: Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708, USA. szhao@cvm.fda.gov
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: D G
authors.initials: DG
authors.lastname: White
authors.forename: P F
authors.initials: PF
authors.lastname: McDermott
authors.forename: S
authors.initials: S
authors.lastname: Friedman
authors.forename: L
authors.initials: L
authors.lastname: English
authors.forename: S
authors.initials: S
authors.lastname: Ayers
authors.forename: J
authors.initials: J
authors.lastname: Meng
authors.forename: J J
authors.initials: JJ
authors.lastname: Maurer
authors.forename: R
authors.initials: R
authors.lastname: Holland
authors.forename: R D
authors.initials: RD
authors.lastname: Walker
mesh_terms: D000818:Animals; D002417:Cattle; D002511:Cephalosporins / Q000494:pharmacology*; D002645:Chickens; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012475:Salmonella / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D013552:Swine; D014422:Turkeys; D019155:Veterinary Drugs / Q000494:pharmacology*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D002511:Cephalosporins; D019155:Veterinary Drugs; C053503:ceftiofur; C103022:beta-lactamase CMY-2; D001618:beta-Lactamases