[The myc oncogene and transdifferentiation of the retinal pigment epithelium].
The retinal pigment epithelium (RPE) develops from the same sheet of neuroepithelium as the neuroretina. When infected with MC29, a v-myc expressing virus, the RPE cells can be induced to transdifferentiate and to take a neuroretinal epithelium fate. After a PCR-based differential screening from these cells we have identified three genes of interest. Qath5, a quail basic helix-loop-helix (bHLH) gene that is closely related to the Drosophila atonal, and whose expression is found in the developing neuroretina. A Chx10-related homeobox gene also expressed in the developing neuroretina and HuD, a RNA-binding protein not expressed in the RPE but expressed during neurogenesis. Beside these genes whose function is involved in regulating neuronal differentiation myc also induced a transient Mitf expression. Mitf is expressed in the entire optic cup, later restricted to the pigmented retina. Mitf is involved in the regulation of the pigmented differentiation. We conclude that v-myc can reverse the RPE to the bipotential retinal primordia.
Beche-Belsot
J S
JS
CNRS/IC UMR 146, Institut Curie Recherche Bâtiment 110, Centre Universitaire 91405 Orsay.
Planque
N
N
Martin
P
P
Saule
S
S
D001355:Alpharetrovirus / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002472:Cell Transformation, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D051959:ELAV Proteins; D005123:Eye / Q000196:embryology; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D016259:Genes, myc; D006133:Growth Substances; D018257:Helix-Loop-Helix Motifs; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D051739:Microphthalmia-Associated Transcription Factor; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D016274:Oncogene Protein p55(v-myc) / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000196:embryology*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D029961:Zebrafish Proteins
D004268:DNA-Binding Proteins; D051959:ELAV Proteins; D005136:Eye Proteins; D006133:Growth Substances; D018398:Homeodomain Proteins; D051739:Microphthalmia-Associated Transcription Factor; D009419:Nerve Tissue Proteins; D016274:Oncogene Protein p55(v-myc); D016601:RNA-Binding Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C413414:atoh7 protein, zebrafish