Effect of human recombinant leptin on lipid handling by fully differentiated Caco-2 cells.
It has been established that leptin displays a number of effects on peripheral tissues. We have investigated the effect of the hormone on lipid synthesis, apolipoprotein biogenesis and lipoprotein secretion in Caco-2 cells. Immunocytochemistry revealed the presence of leptin receptors (Ob-Rb) on the basolateral membrane. Incubation of cells with 200 nM leptin resulted in a decreased export of triglycerides in the basolateral medium without affecting monoglyceride, diglyceride and cholesterol ester lipid classes. It also significantly reduced the output of de novo-synthesized apolipoprotein (Apo)B-100 and ApoB-48 as well as that of newly formed chylomicrons and of low-density lipoproteins. It also enhanced that of ApoA-I, ApoA-IV and ApoE. Our results support the hypothesis that leptin can affect energy balance at the gut level by reducing lipid release into the circulation.
Stan
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S
Centre de recherche, Hôpital Ste-Justine, 3175 Côte Ste-Catherine, Montreal, Quebec, Canada HST 1C5.
Levy
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E
Bendayan
M
M
Zoltowska
M
M
Lambert
M
M
Michaud
J
J
Asselin
C
C
Delvin
E E
EE
D001053:Apolipoproteins / Q000378:metabolism; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000378:metabolism; D016764:Cell Polarity; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D020738:Leptin / Q000235:genetics / Q000494:pharmacology*; D050356:Lipid Metabolism; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology
D001053:Apolipoproteins; D002352:Carrier Proteins; C515448:LEPR protein, human; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D011994:Recombinant Proteins