Intracellular trafficking/membrane targeting of human reduced folate carrier expressed in Xenopus oocytes.
The major cellular pathway for uptake of the vitamin folic acid, including its absorption in the intestine, is via a plasma membrane carrier system, the reduced folate carrier (RFC). Very little is known about the mechanisms that control intracellular trafficking and plasma membrane targeting of RFC. To begin addressing these issues, we used Xenopus oocyte as a model system and examined whether the signal that targets the protein to the plasma membrane is located in the COOH-terminal cytoplasmic tail or in the backbone of the polypeptide. We also examined the role of microtubules and microfilaments in intracellular trafficking of the protein. Confocal imaging of human RFC (hRFC) fused to the enhanced green fluorescent protein (hRFC-EGFP) showed that the protein was expressed at the plasma membrane, with expression confined almost entirely to the animal pole of the oocyte. Localization of hRFC at the plasma membrane was not affected by partial or total truncation of the COOH-terminal tail of the polypeptide, whereas a construct of the cytoplasmic tail fused to EGFP was not found at the plasma membrane. Disruption of microtubules, but not microfilaments, prevented hRFC expression at the plasma membrane. These results demonstrate that the molecular determinant(s) that directs plasma membrane targeting of hRFC is located within the backbone of the polypeptide and that intact microtubules, but not microfilaments, are essential for intracellular trafficking of the protein.
Subramanian
V S
VS
Department of Veterans Affairs Medical Center, Long Beach, CA 90822, USA.
Marchant
J S
JS
Parker
I
I
Said
H M
HM
D008841:Actin Cytoskeleton / Q000502:physiology; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D006801:Humans; D002955:Leucovorin / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D008845:Microinjections; D008870:Microtubules / Q000502:physiology; D009865:Oocytes / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D058978:Reduced Folate Carrier Protein; D015398:Signal Transduction; D014162:Transfection; D014316:Tritium; D014982:Xenopus laevis
D002352:Carrier Proteins; D008164:Luminescent Proteins; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; D058978:Reduced Folate Carrier Protein; C548699:SLC19A1 protein, human; D014316:Tritium; D049452:Green Fluorescent Proteins; D015638:Cytochalasin D; D002955:Leucovorin