title: Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development.
abstract: Arabidopsis possesses several genes related to the multidrug resistance (MDR) genes of animals, one of which, AtMDR1, was shown to be induced by the hormone auxin. Plants having mutations in AtMDR1 or its closest relative, AtPGP1, were isolated by a reverse genetic strategy. Auxin transport activity was greatly impaired in atmdr1 and atmdr1 atpgp1 double mutant plants. Epinastic cotyledons and reduced apical dominance were mutant phenotypes consistent with the disrupted basipetal flow of auxin. The auxin transport inhibitor 1-naphthylphthalamic acid was shown to bind tightly and specifically to AtMDR1 and AtPGP1 proteins. The results indicate that these two MDR-like genes of Arabidopsis encode 1-naphthylphthalamic acid binding proteins that are required for normal auxin distribution and auxin-mediated development.
authors.affiliation: Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison, Wisconsin 53706, USA.
authors.forename: B
authors.initials: B
authors.lastname: Noh
authors.forename: A S
authors.initials: AS
authors.lastname: Murphy
authors.forename: E P
authors.initials: EP
authors.lastname: Spalding
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D029688:Brassica napus / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D017931:DNA Primers; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D009579:Nitrobenzoates / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism; D021381:Protein Transport; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D017931:DNA Primers; D007210:Indoleacetic Acids; D009579:Nitrobenzoates; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid