[Human hR24L gene is involved in DNA excision repair and recombination repair].
OBJECTIVE
To study the correction phenotype of temperature sensitive mutant rad24LR of S. cerevisiae and to clone wild human functional homologue.


METHODS
The temperature sensitive mutant rad24LR was studied by complementation test. Transformation of rad24LR was performed with human cDNA expression library to screen for reversion phenotype.


RESULTS
RAD24L can complement the radiation sensitive phenotype of the mutation. Its homologue of human cDNA was cloned by screening transformed rad24LR with human expression library for reversion phenotype.


CONCLUSIONS
Human DNA repair gene hR24L was successfully cloned in this study. The product hR24L is involved in both DNA excision repair and recombination repair.
Han
Y
Y
Laboratory of Molecular Biology, Laboratory of Cancer and Radiation Diseases, Third Hospital of Beijing Medical University, Beijing 100083.
Zhu
Y
Y
D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D016364:Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal