title: Automation of fluorescence-based PCR for confirmation of meningococcal disease.
abstract: A fluorescence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.
authors.affiliation: Scottish Meningococcus and Pneumococcus Reference Laboratory, Glasgow, United Kingdom.
authors.forename: M A
authors.initials: MA
authors.lastname: Diggle
authors.forename: G F
authors.initials: GF
authors.lastname: Edwards
authors.forename: S C
authors.initials: SC
authors.lastname: Clarke
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004268:DNA-Binding Proteins; D005453:Fluorescence; D006801:Humans; D008589:Meningococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012371:Robotics; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; C097706:CtrA protein, Caulobacter; D004268:DNA-Binding Proteins; D014157:Transcription Factors