[Deletions and aberrant transcription of p16 and p15 genes in childhood acute lymphoblastic leukemia].
OBJECTIVE
To explore the role of p16(MTS1) and p15(MTS2) genes in the pathogenesis of childhood acute lymphoblastic leukemia(ALL).


METHODS
PCR and Southern blot were used to analyse p16 and p15 gene deletions, RT-PCR was used to analyse p15 gene transcription.


RESULTS
Exon 1 and 2 of p16 gene deletion was detected in 8 of 21 patients(38.1%), and exon 1 and 2 of p15 gene deletion in 11 of 21 patients(52.3%). Aberrant p15 gene transcription was observed in two cases.


CONCLUSIONS
High frequency of p16 and p15 gene deletions and aberrant transcription may be involved in the pathogenesis of childhood ALL.
Fu
G
G
Department of Immunology, China Medical University, Shenyang 110001.
Lu
C
C
Zhang
R
R
D000293:Adolescent; D018797:Cell Cycle Proteins; D002648:Child; D002675:Child, Preschool; D050762:Cyclin-Dependent Kinase Inhibitor p15; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D007223:Infant; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins