title: Protection of N-methyl-N'-nitro-N-nitrosoguanidine-induced in vivo clastogenicity by aqueous garlic extract.
abstract: The modulatory effects of garlic extract on the in vivo clastogenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a carcinogenic nitrosamine, were evaluated by quantification of micronuclei and chromosomal aberrations in metaphase cells from the bone marrow of male Wistar rats. A single intraperitoneal injection of MNNG (40 mg/kg bodyweight) was found to be clastogenic as revealed by the increased frequency of micronucleated polychromatic erythrocytes and chromosomal aberrations. Pretreatment with aqueous garlic extract (250 mg/kg bodyweight) for 5 days significantly reduced the frequencies of MNNG-induced micronuclei and chromosomal aberrations. The results demonstrate that administration of garlic extract protects against the clastogenic effects of MNNG.
authors.affiliation: Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Arivazhagan
authors.forename: S
authors.initials: S
authors.lastname: Nagini
authors.forename: S T
authors.initials: ST
authors.lastname: Santhiya
authors.forename: A
authors.initials: A
authors.lastname: Ramesh
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000187:drug effects; D005737:Garlic; D008297:Male; D008677:Metaphase / Q000187:drug effects; D008769:Methylnitronitrosoguanidine / Q000008:administration & dosage / Q000633:toxicity*; D015162:Micronucleus Tests; D016296:Mutagenesis / Q000187:drug effects*; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000517:prevention & control
chemical_list: D009153:Mutagens; D010936:Plant Extracts; D008769:Methylnitronitrosoguanidine