title: The Ded1 DEAD box helicase interacts with Chk1 and Cdc2.
abstract: Ded1 is a fission yeast DEAD box protein involved in translation. We isolated Ded1 in a screen for multi-copy suppressors of a cold-sensitive, loss-of-function mutant of the cyclin-dependent kinase Cdc2. The checkpoint protein kinase Chk1, required for cell cycle arrest in response to DNA damage, was also isolated in this screen. Ded1 interacts with Chk1 in a two-hybrid screen, and this physical interaction can be recapitulated in Schizosaccharomyces pombe. The Ded1 polypeptide is modified in response to heat shock and depletion of carbon source. These two stressors appear to cause different modifications. Thus, the Ded1 protein appears to respond to particular types of cellular stress and may influence the activity of Cdc2 as a result.
authors.affiliation: Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.
authors.forename: Hsing-Yin
authors.initials: HY
authors.lastname: Liu
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Nefsky
authors.forename: Nancy C
authors.initials: NC
authors.lastname: Walworth
mesh_terms: D016203:CDC2 Protein Kinase / Q000737:chemistry / Q000378:metabolism*; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D000071877:Checkpoint Kinase 1; D053487:DEAD-box RNA Helicases; D004249:DNA Damage; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006358:Hot Temperature; D015151:Immunoblotting; D020935:MAP Kinase Signaling System; D008954:Models, Biological; D009154:Mutation; D010641:Phenotype; D011485:Protein Binding; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020365:RNA Helicases; D012568:Schizosaccharomyces / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction; D013696:Temperature; D013997:Time Factors; D020798:Two-Hybrid System Techniques; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; D000071877:Checkpoint Kinase 1; C000605873:Chk1 protein, S pombe; D016203:CDC2 Protein Kinase; C492151:sum3 protein, S pombe; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases