Triacylglycerol-rich lipoproteins interact with human vascular cells in a lipid-dependent fashion.
Plasma triacylglycerol-rich lipoproteins (TRL) are being considered as a key lipid fraction in the pathogenesis of atherosclerotic cardiovascular disease. Here we compared the influence of two monounsaturated oils [virgin olive oil (VOO) and high-oleic sunflower oil (HOSO)] on the capability of postprandial TRL to interact with two human vascular cell lines [umbilical vein endothelial (HUVEC) and aorta smooth muscle (HASMC) cells]. A fluorescent probe was used for labeling TRL and to determine receptor activity of HUVEC and HASMC. The values for total cell-associated, bound, and internalized TRL were higher in HUVEC, and TRL from VOO was the better ligand recognized but at lower affinity than TRL from HOSO. There was a competitive effect of very low density lipoproteins (VLDL) for the uptake of TRL by cells, which was found to be dependent on the origin/lipid composition of the ligands and cell-type specific. We also conclude that the VLDL receptor (VLDLr) may contribute significantly to the HASMC binding capacity for postprandial TRL mediated by lipoprotein lipase (LPL) or LPL-binding molecules. Our findings are compatible with a selective role of the clustered O-linked sugar domain of the VLDLr in the catabolism of TRL by human vascular cells.
Pacheco
Y M
YM
Instituto de la Grasa, CSIC, 41012 Sevilla, Spain.
Abia
R
R
Perona
J S
JS
Reina
M
M
Ruiz-Gutiérrez
V
V
Montero
E
E
Muriana
F J
FJ
D000328:Adult; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008297:Male; D011973:Receptors, LDL / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; D014280:Triglycerides; C036053:VLDL receptor