title: [Mechanism of selenium protecting against free radical damages during myocardial ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To investigate the mechanism of selenium (Se) protecting against free radical damages during myocardial ischemia/reperfusion (I/R) in rats.
  
  
  METHODS
  All rats were separated into three groups: control, organic Se and inorganic Se supplemented groups. Myocardial I/R was established by operation in all three groups. Biochemical assays, atomic absorption spectrophotometry, RT-PCR and cDNA sequence scanning were performed to examine the changes in myocardial MDA level, GSH-Px activity and gene expression, GSH-Px cDNA nucleotide sequence, as well as Se and Ca2+ and Mg2+ concentrations in the heart muscle during I/R.
  
  
  RESULTS
  The administration of Se for 7 days did not significantly change the content of Se in the plasma and blood cells. In the two Se-supplemented groups, after reperfusion for 30 minutes, myocardial Se concentration and GSH-Px activity were increased significantly, and GSH-Px expression was elevated with a normal cDNA sequence, and at the same time, MDA contents in the two Se-supplemented groups were lower than that in the control group.
  
  
  CONCLUSIONS
  Se supplementation can protect against free radical damages during myocardial I/R by increasing myocardial Se content and improving both the expression and activity of GSH-Px.
authors.affiliation: Beijing Institute of Heart Lung & Blood Vessel Diseases, Beijing 100029.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Bai
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D017202:Myocardial Ischemia / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D051381:Rats; D012643:Selenium / Q000494:pharmacology*
chemical_list: D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D012643:Selenium