Changes in the glutamate release and uptake of cerebellar cells in perinatally nicotine-exposed rat pups.
Cerebellar granule and glial cells were cultured from 7 day-old rat pups after pre- and post-natal nicotine treatment. Ten days later, the basal release of glutamate in the granule cells prepared from the pre- and post-natally nicotine-exposed pups was higher and lower than the controls, respectively. The N-methyl-D-aspartate-induced release of glutamate was higher in the granule cells of post-natal nicotine exposed rats. However, the nicotine-induced glutamate release was either unchanged or was lower in the granule cells of all nicotine-treated pups. The basal glutamate uptake was higher in the glial cells from those exposed pre-natally and lower in the continuously nicotine-exposed pups. The sensitivities of L-trans-pyrrolidine-2,4-dicarboxylic acid on glutamate uptake were higher in all nicotine treated groups. There was a higher number of specific [3H]dizocilpine binding sites in the pre- or continuously nicotine-exposed group. These results suggest that the cerebellar cell properties are altered after perinatal nicotine exposure and that the development of an excitatory amino acid system might be affected differently depending on the nicotine exposure time.
Lim
D K
DK
College of Pharmacy and Institute for Drug Development, Chonnam National University, Bukgu, Kwangju, Korea. dklim@chonnam.chonnam.ac.kr
Kim
H S
HS
D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000493:pharmacokinetics / Q000494:pharmacology; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D016202:N-Methylaspartate / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009538:Nicotine / Q000493:pharmacokinetics / Q000494:pharmacology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
D018698:Glutamic Acid; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D009538:Nicotine