title: Isolation and sequencing of infectious clones of feline foamy virus and a human/feline foamy virus Env chimera.
abstract: Full-length DNAs of the Coleman and S7801 strains (pSKY3.0, pSKY5.0) of infectious feline foamy viruses (FFVs) were cloned and sequenced. Parental viruses, designated SKY3.0 and SKY5.0, were secreted following transfection of Crandell feline kidney (CRFK) cells. Production of the rescued parental viruses was enhanced in the presence of trichostatin A. Amino acid sequence similarities between FFV and human foamy virus (HFV) are extremely low for the envelope protein and capsid antigen, as predicted from the two clones. However, a chimeric FFV clone was constructed with the HFV Env substituted for the FFV Env. The chimeric virus (HFFV, SKY4.0) was able to infect and replicate in CRFK cells as well as in peripheral blood mononuclear cells of cats in vivo. Consequently, the chimeric HFFV may be useful for the creation of FV vectors for gene transfer strategies.
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan3.|Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hatama
authors.affiliation: Department of Immunology, National Institute of Infectious Disease, Tokyo 113-8657, Japan2.
authors.forename: Kaori
authors.initials: K
authors.lastname: Otake
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Shinya
authors.initials: S
authors.lastname: Omoto
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Murase
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Atushi
authors.initials: A
authors.lastname: Ikemoto
authors.affiliation: Central Laboratory, Kyoritu Shouji Co. Ltd, Ibaraki, Japan4.
authors.forename: Masami
authors.initials: M
authors.lastname: Mochizuki
authors.affiliation: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan3.
authors.forename: Eiji
authors.initials: E
authors.lastname: Takahashi
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Harumi
authors.initials: H
authors.lastname: Okuyama
authors.affiliation: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan1.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Fujii
mesh_terms: D000818:Animals; D002213:Capsid / Q000235:genetics; D002371:Cat Diseases / Q000097:blood / Q000821:virology*; D002415:Cats; D002460:Cell Line / Q000821:virology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification*; D005822:Genetic Vectors; D015726:Giant Cells / Q000821:virology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D009000:Monocytes / Q000821:virology; D011995:Recombination, Genetic; D012192:Retroviridae Infections / Q000662:veterinary*; D016092:Spumavirus / Q000235:genetics* / Q000472:pathogenicity; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D006877:Hydroxamic Acids; D014759:Viral Envelope Proteins; C012589:trichostatin A