title: Evidence for evolution of canine parvovirus type 2 in Italy.
abstract: Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pratelli
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Maria
authors.initials: M
authors.lastname: Tempesta
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cavalli
authors.affiliation: Department of Pathology and Infectious Diseases, University of Messina, Messina, Italy2.
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Bozzo
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1.
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.affiliation: James Baker Institute, Cornell University, Ithaca, NY, USA3.
authors.forename: Leland
authors.initials: L
authors.lastname: Carmichael
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002213:Capsid / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D003967:Diarrhea / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics* / Q000276:immunology; D004285:Dogs; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D019143:Evolution, Molecular; D014644:Genetic Variation; D007558:Italy; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000235:genetics* / Q000276:immunology; D012150:Polymorphism, Restriction Fragment Length; D020539:Sequence Analysis, Protein
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers; D000939:Epitopes