5'-bis-pyrenylated oligonucleotides display enhanced excimer fluorescence upon hybridization with DNA and RNA.
A simple one-step procedure was applied for synthesis of oligonucleotide conjugates bearing two pyrene residues at the 5'-phosphate of oligonucleotide. Excimer fluorescence intensity of the conjugates is highly sensitive to duplex formation: binding of the bis-pyrenylated oligonucleotides to their DNA and RNA targets leads 10-fold increase of fluorescence. The data show that excimer fluorescence intensity of the conjugates depends linearly on the concentration of target DNA and permits quantification of DNA in solution.
Kostenko
E
E
Novosibirsk Institute of Bioorganic Chemistry, Russia.
Dobrikov
M
M
Komarova
N
N
Pyshniy
D
D
Vlassov
V
V
Zenkova
M
M
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012313:RNA; D004247:DNA