[Effects of fungal elicitor on cell status and taxol production in cells suspension cultures of Taxus chinesis var. mairei].
Effects of fungal elicitor on cell redox status and taxol production were studied in suspension cultures of Taxus chinesis var. mairei in the late exponential stage. The results show that fungal elicitor induced oxygen burst, changes of the cell redox status, alkalinization of medium and the fluctuation of the activity of redox enzymes with a sequence. The content of protein representing the quantity of enzymes increased. The activity of SOD increased quickly after treatment by fungal elicitor and that of POD could be kept at a higher level in contrast to the control. The activity of CAT was inhibited at first and followed by an obvious increase, while the activity of PAL was promoted. The taxol yield was 5 folds of the control, reaching to 67 mg/L.
Zhang
C P
CP
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Li
C
C
Yuan
Y J
YJ
Sun
A C
AC
Hu
C X
CX
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D002374:Catalase / Q000378:metabolism; D005670:Fusarium / Q000502:physiology*; D010084:Oxidation-Reduction; D017239:Paclitaxel / Q000096:biosynthesis*; D010544:Peroxidases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013535:Suspensions; D020946:Taxus / Q000166:cytology / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D013535:Suspensions; D010544:Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D017239:Paclitaxel