title: Crosstalk of prolyl isomerases, Pin1/Ess1, and cyclophilin A.
abstract: Previous studies have indicated that Ess1/Pin1, a gene in the parvulin family of peptidyl-prolyl isomerases (PPIases), plays an important role in regulating the G(2)/M transition of the cell cycle by binding cell-cycle-regulating proteins in eukaryotic cells. Although the ess1 gene has been considered to be essential in yeast, we have isolated viable ess1 deletion mutants and demonstrated, via analysis of yeast gene expression profiles using microarray techniques, a novel regulatory role for ESS1 in the G(1) phase. Although the overall expression profiles in the tested strains (C110-1, W303, S288c, and RAY-3AD) were similar, marked changes were detected for a number of genes involved in the molecular action of ESS1. Among these, the expression levels of a cyclophilin A gene, also a member of the PPIase family, increased in the ess1 null mutant derived from C110-1. Subsequent treatment with cyclosporin A significantly retarded growth, which suggests that ESS1 and cyclophilin A are functionally linked in yeast cells and play important roles at the G(1) phase of the cell cycle.
authors.affiliation: Department of Biological Science & Technology, Faculty of Industrial Science & Technology, Science University of Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Fujimori
authors.forename: W
authors.initials: W
authors.lastname: Gunji
authors.forename: J
authors.initials: J
authors.lastname: Kikuchi
authors.forename: T
authors.initials: T
authors.lastname: Mogi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohashi
authors.forename: T
authors.initials: T
authors.lastname: Makino
authors.forename: A
authors.initials: A
authors.lastname: Oyama
authors.forename: K
authors.initials: K
authors.lastname: Okuhara
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
mesh_terms: D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology; D021984:Cyclophilin A / Q000235:genetics* / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D016193:G1 Phase / Q000502:physiology; D017353:Gene Deletion; D015870:Gene Expression; D005800:Genes, Fungal; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D020411:Oligonucleotide Array Sequence Analysis; D019696:Peptidylprolyl Isomerase / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004271:DNA, Fungal; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D021984:Cyclophilin A; C000606845:ESS1 protein, S cerevisiae; D019696:Peptidylprolyl Isomerase