Isolation and characterisation of the ylmE homologue of Thermus thermophilus.
Screening of a Thermus thermophilus genomic library led to the identification of a homologue of the ylmE gene. ylmE is highly conserved in widely divergent organisms from prokaryotes to mammals, suggesting an important, albeit currently unknown, cellular function. The 633 bp gene has a GC content of 69.2% overall and 90% in the third nucleotide position, while the gene product is predicted to be a soluble cytoplasmic protein of 23,441 Da. It belongs to a family of conserved proteins of unknown function and exhibits amino acid identities ranging from 45% to 28% to the Aquifex aeolicus and Saccharomyces cerevisiae family members, respectively. We speculate that the gene product may be involved in a cellular stress response in T. thermophilus.
Spada
S
S
Industrial Biochemistry Group, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.
Gibert
Y
Y
Pembroke
J T
JT
Wall
J G
JG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016963:Thermus thermophilus / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C445039:ylmE protein, Thermus thermophilus