Crystallization and preliminary X-ray analysis of the periplasmic nitrate reductase (NapA-NapB complex) from Rhodobacter sphaeroides f. sp. denitrificans.
The periplasmic nitrate reductase of Rhodobacter sphaeroides f. sp. denitrificans is a heterodimer responsible for the first step of reduction in the denitrification process by the conversion of nitrate to nitrite. It consists of a 91 kDa molybdenum-containing catalytic subunit (NapA) and a 17 kDa dihaem cytochrome c (NapB). Crystals of the NapA-NapB complex were obtained by the vapour-diffusion method using ammonium sulfate as precipitant. They belong to the P6(1)22 space group, with unit-cell parameters a = b = 151.9, c = 255.8 A, and contain a single complex in the asymmetric unit. A complete native data set was collected at a synchrotron source to 3.1 A resolution.
Pignol
D
D
CEA/Cadarache, DSV, DEVM, Laboratoire de Bioénergétique Cellulaire, 13108 St Paul lez Durance CEDEX, France. david.pignol@cea.fr
Adriano
J M
JM
Fontecilla-Camps
J C
JC
Sabaty
M
M
D055516:2S Albumins, Plant; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012242:Rhodobacter sphaeroides / Q000201:enzymology*
D055516:2S Albumins, Plant; D010940:Plant Proteins; D011994:Recombinant Proteins; C050363:napin protein, Brassica napus; D009565:Nitrate Reductases; D050901:Nitrate Reductase