[Establishment and characterization of a cell line derived from human ovarian mucinous cystadenocarcinoma].
OBJECTIVE
To establish a cell line of human ovarian cancer, and study its characterization.


METHODS
The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno-histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc.


RESULTS
A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations, alcian blue periobic acid-schiff (AbPAS), mucicarmine, alcian blue stainings, estradiol (E2) and progesterone were all positive. Without being polluted, it was sensitive to polivirus-I, adenovirus 7 and measles virus.


CONCLUSIONS
OMC685 is a distinct human ovarian tumous cell line.
Wan
Q
Q
Department of Parasitology, Zunyi Medical College, Zunyi 563003, China.
Xu
D
D
Li
Z
Z
D002452:Cell Count; D018929:Cell Culture Techniques; D048429:Cell Size; D018282:Cystadenocarcinoma, Mucinous / Q000473:pathology*; D005260:Female; D006728:Hormones / Q000378:metabolism; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology*
D006728:Hormones