title: MCF-10A-NeoST: a new cell system for studying cell-ECM and cell-cell interactions in breast cancer.
abstract: OBJECTIVE
  There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers.
  
  
  METHODS
  We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer.
  
  
  RESULTS
  NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells.
  
  
  CONCLUSIONS
  MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
authors.affiliation: Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47905, USA.
authors.forename: N D
authors.initials: ND
authors.lastname: Zantek
authors.forename: J
authors.initials: J
authors.lastname: Walker-Daniels
authors.forename: J
authors.initials: J
authors.lastname: Stewart
authors.forename: R K
authors.initials: RK
authors.lastname: Hansen
authors.forename: D
authors.initials: D
authors.lastname: Robinson
authors.forename: H
authors.initials: H
authors.lastname: Miao
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: H J
authors.initials: HJ
authors.lastname: Kung
authors.forename: M J
authors.initials: MJ
authors.lastname: Bissell
authors.forename: M S
authors.initials: MS
authors.lastname: Kinch
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D002448:Cell Adhesion / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D048429:Cell Size / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013997:Time Factors
chemical_list: D011505:Protein-Tyrosine Kinases