A single extraction step in the quantitative analysis of arbutin in bearberry (Arctostaphylos uva-ursi) leaves by high-performance liquid chromatography.
A fast and simple extraction procedure coupled with a simple HPLC method has been developed in order to determine the arbutin content of leaves of Arctostaphylos uva-ursi plants grown at four different geographical sites and collected during two different seasons. Using the optimised analytical system, the arbutin content of bearberry leaves was found to vary from 6.30 to 9.16% expressed on a dry weight basis. Autumn is shown to be a better period than spring for the collection of plant material in order to obtain the highest yield of arbutin.
Parejo
I
I
Department of Natural Products, Plant Biology and Edaphology, Faculty of Pharmacy, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Catalonia, Spain.
Viladomat
F
F
Bastida
J
J
Codina
C
C
D001104:Arbutin / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029791:Ericaceae / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D001104:Arbutin