title: [Studies on hematopoietic reconstitution by ex vivo expanded human cord blood hematopoietic stem/progenitor cells in SCID mice].
abstract: OBJECTIVE
  To elucidate the effect of the combination of cytokines on ex vivo expansion and self-renewal potential of human umbilical cord blood hematopoietic stem/progenitor cells.
  
  
  METHODS
  CD34+ cells from cord blood were expanded for 14 days in the culture containing FL + Tpo + SCF + IL-6 + IL-3 + G-CSF and the expanded cells were transplanted into sublethally irradiated SCID mice.
  
  
  RESULTS
  The expanded cells were engrafted smoothly in the SCID recipients and reconstituted their hematopoiesis. Furthermore, human hematopoietic cells could be detected in the marrow of the recipients 6 weeks after transplantation.
  
  
  CONCLUSIONS
  It is possible to expand hematopoietic cells ex vivo efficiently and maintain concomitantly their self-renewal and hematopoietic reconstitution capacities by the combination of FL + Tpo + SCF + IL-6 + IL-3 + G-CSF.
authors.affiliation: General Hospital of PLA, Beijing 100853.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Pei
authors.forename: S
authors.initials: S
authors.lastname: Lu
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000494:pharmacology; D051379:Mice; D016513:Mice, SCID; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D008565:Membrane Proteins; C084881:flt3 ligand protein; D013926:Thrombopoietin