The human temporal cortex: characterization of neurons expressing nitric oxide synthase, neuropeptides and calcium-binding proteins, and their glutamate receptor subunit profiles.
Immunocytochemical techniques were used to examine the distribution of neurons immunoreactive (-ir) for nitric oxide synthase (nNOS), somatostatin (SOM), neuropeptide Y (NPY), parvalbumin (PV), calbindin (CB) and calretinin (CR), in the inferotemporal gyrus (Brodmann's area 21) of the human neocortex. Neurons that colocalized either nNOS or SOM with PV, CB or CR were also identified by double-labeling techniques. Furthermore, glutamate receptor subunit profiles (GluR1, GluR2/3, GluR2/4, GluR5/6/7 and NMDAR1) were also determined for these cells. The number and distribution of cells containing nNOS, SOM, NPY, PV, CB or CR differed for each antigen. In addition, distinct subpopulations of neurons displayed different degrees of colocalization of these antigens depending on which antigens were compared. Moreover, cells that contained nNOS, SOM, NPY, PV, CB or CR expressed different receptor subunit profiles. These results show that specific subpopulations of neurochemically identified nonpyramidal cells may be activated via different receptor subtypes. As these different subpopulations of cells project to specific regions of pyramidal cells, facilitation of subsets of these cells via different receptor subunits may activate different inhibitory circuits. Thus, various distinct, but overlapping, inhibitory circuits may act in concert in the modulation of normal cortical function, plasticity and disease.
González-Albo
M C
MC
Instituto Cajal (CSIC), Avenida Dr Arce, 37, 28002 Madrid, Spain. defelipe@cajal.csic.es
Elston
G N
GN
DeFelipe
J
J
D000328:Adult; D000918:Antibody Specificity; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology; D009478:Neuropeptide Y / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052248:Nitric Oxide Synthase Type I; D010320:Parvalbumins / Q000032:analysis / Q000276:immunology; D018091:Receptors, AMPA / Q000032:analysis; D017470:Receptors, Glutamate / Q000032:analysis*; D018092:Kainic Acid Receptors / Q000032:analysis; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000276:immunology; D013004:Somatostatin / Q000032:analysis*; D013702:Temporal Lobe / Q000166:cytology
D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins; D009478:Neuropeptide Y; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D010320:Parvalbumins; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D016194:Receptors, N-Methyl-D-Aspartate; D064030:S100 Calcium Binding Protein G; D013004:Somatostatin; C576764:CALB2 protein, human; C496320:NOS1 protein, human