Biogenesis of respiratory complex I.
Proteins specifically involved in the biogenesis of respiratory complex I in eukaryotes have been characterized. The complex I intermediate associated proteins CIA30 and CIA84 are tightly bound to an assembly intermediate of the membrane arm. Like chaperones, they are involved in multiple rounds of membrane arm assembly without being part of the mature structure. Two biosynthetic subunits of eukaryotic complex I have been characterized. The acyl carrier subunit is needed for proper assembly of the peripheral arm as well as the membrane arm of complex I. It may interact with enzymes of a mitochondrial fatty acid synthetase. The 39/40-kDa subunit appears to be an isomerase with a tightly bound NADPH. It is related to a protein family of reductases/isomerases. Both subunits have been discussed to be involved in the synthesis of a postulated, novel, high-potential redox group.
Schulte
U
U
Institute of Biochemistry, Heinrich-Heine-University, Düsseldorf, Germany. ulrich.schulte@uni-duesseldorf.de
D000213:Acyl Carrier Protein / Q000737:chemistry / Q000235:genetics; D042967:Electron Transport Complex I; D005800:Genes, Fungal; D008928:Mitochondria / Q000201:enzymology; D008958:Models, Molecular; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics; D010084:Oxidation-Reduction; D010802:Phylogeny; D021122:Protein Subunits
D000213:Acyl Carrier Protein; D021122:Protein Subunits; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I