title: [Evaluation of chemosensitivity testing by CD-DST, and TS and DPD activity in cases of colorectal liver].
abstract: We evaluated the usefulness of the following three in vitro assays in cases of resected colorectal liver metastases. Chemosensitivity by collagen gel droplet drug sensitivity test (CD-DST) was very low in all cases, suggesting this method is not predictive for this disease. In contrast, thymidylate synthetase (TS) activity and dihydropyrimidine dehydrogenase (DPD) activity in tumor tissue were high in many patients with recurrent disease. Thus, these enzyme activities are promising for assessment of clinical outcome following hepatic resection of colorectal liver metastases. Further analyses with large numbers of cases are needed to determine the significance of these in vitro studies.
authors.affiliation: Dept. of Surgery and Radiology, NTT West Osaka Hospital.
authors.forename: T
authors.initials: T
authors.lastname: Tono
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Kanoh
authors.forename: Y
authors.initials: Y
authors.lastname: Nakano
authors.forename: S
authors.initials: S
authors.lastname: Tamagaki
authors.forename: A
authors.initials: A
authors.lastname: Yasue
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Matsui
authors.forename: T
authors.initials: T
authors.lastname: Iwazawa
authors.forename: H
authors.initials: H
authors.lastname: Yano
authors.forename: M
authors.initials: M
authors.lastname: Kinuta
authors.forename: J
authors.initials: J
authors.lastname: Okamura
authors.forename: T
authors.initials: T
authors.lastname: Monden
mesh_terms: D015179:Colorectal Neoplasms / Q000473:pathology*; D042943:Dihydrouracil Dehydrogenase (NADP); D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000556:secondary*; D010088:Oxidoreductases / Q000378:metabolism*; D015996:Survival Rate; D013940:Thymidylate Synthase / Q000378:metabolism*
chemical_list: D010088:Oxidoreductases; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase