title: Expression of TAR RNA-Binding Protein in Baculovirus and Co-Immunoprecipitation with Insect Cell Protein Kinase.
abstract: TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immunoprecipitates with a protein in these cells antigenically related to human PKR. Copyright 1995 S. Karger AG, Basel
authors.affiliation: Wellcome Research Laboratories, Beckenham, UK.
authors.forename: E.D.
authors.initials: ED
authors.lastname: Blair
authors.forename: C.M.
authors.initials: CM
authors.lastname: Roberts
authors.forename: B.W.
authors.initials: BW
authors.lastname: Snowden
authors.forename: A.
authors.initials: A
authors.lastname: Gatignol
authors.forename: M.
authors.initials: M
authors.lastname: Benkirane
authors.forename: K.-T.
authors.initials: KT
authors.lastname: Jeang