[Dynamic variance of intracellular metabolic energies under rhythmical control for dissolved oxygen in PHB mixed cultivation].
The mixed cultivation using cheaper carbon source-wasted food material contained glucose and lactate at the same time was conducted in 5L fermentor, within which glucose was converted to lactate by L. delbrueckii in anaerobic condition and the lactate was converted to PHB by R. eutropha in aerobic condition. Considering dissolved oxygen concentration may affect the level of intracellular ATP and NADPH of the metabolic pathways for R. eutropha in lactate under autotrophy or heterotrophy, rhythmical oscillated control for DO based on chaos control method was consequently presented. This method was employed to satisfy two strains for opposite oxygen preferences, moreover, excite the intracellular metabolic energy simultaneously. The values examined through spectrophotofluorimetry represented that both ATP and NADPH exhibited fluctuations in accordance with the DO rhythm. By means of this control design, the concentration of PHB can be doubled than the usual under stable DO control.
Qian
Z W
ZW
National Laboratory of Biochemical Engineering of China, Beijing 100080, China. zwqian@263.net
Tohyama
M
M
Hua
Q
Q
Shimizu
K
K
D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D011108:Polymers / Q000378:metabolism
D011108:Polymers; D019344:Lactic Acid; D009249:NADP; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen; D020155:3-Hydroxybutyric Acid