title: Binding of proteolytically processed phospholipase D from Streptomyces chromofuscus to phosphatidylcholine membranes facilitates vesicle aggregation and fusion.
abstract: Ca(2+)-dependent phospholipase D is secreted from Streptomyces chromofuscus as an intact enzyme of 57 kDa (PLD(57)). Under certain growth conditions, PLD is proteolytically cleaved and activated to form PLD(42/20) (named for the apparent size of the peptides). The PLD(42) catalytic core and 20 kDa C-terminal domain remain tightly associated through noncovalent interactions. In the presence of Ba(2+) (to enhance protein binding to zwitterionic vesicles without hydrolysis of substrate), PLD(42/20), but not PLD(57), induces POPC vesicle leakiness as measured by entrapped CF leakage. PLD(42/20) also induces vesicle fusion (as measured by light scattering, fluorescence quenching, and cryo-TEM) under these conditions (1 mM POPC, 5 mM Ba(2+)); neither PLD(42) nor PLD(20) alone can act as a fusogen. For intact PLD(57) to cause CF leakiness, the soluble activator diC(4)PA must be present. However, even with diC(4)PA, PLD(57) does not induce significant vesicle fusion. In the absence of metal ions, all PLD forms bind to PC vesicles doped with 10 mol % PA. Again, only PLD(42/20) is fusogenic and causes aggregation and fusion on a rapid time scale. Taken together, these data suggest that activated PLD(42/20) inserts more readily into the lipid bilayer than other PLD forms and creates structures that allow bilayers to fuse. Cleavage of the PLD(57) by a secreted protease to generate PLD(42/20) occurs in the late stages of S. chromofuscus cell cultures. Production of this more active and fusogenic enzyme may play a role in nutrient scavenging in stationary phase cultures.
authors.affiliation: Department of Physiology, Boston University, Boston, Massachusetts 02118, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Stieglitz
authors.forename: B A
authors.initials: BA
authors.lastname: Seaton
authors.forename: M F
authors.initials: MF
authors.lastname: Roberts
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001464:Barium / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D020285:Cryoelectron Microscopy; D010450:Endopeptidases / Q000378:metabolism*; D004735:Energy Transfer; D006868:Hydrolysis; D008024:Ligands; D008027:Light; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008561:Membrane Fusion; D010712:Phosphatidic Acids / Q000737:chemistry / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D010739:Phospholipase D / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013302:Streptomyces / Q000201:enzymology*
chemical_list: C086969:1-oleoyl-2-((N-(7-(nitro)-2,1,3-benzoxadiazol-4-yl)amino)hexanoyl)glycero-3-phosphate; D008024:Ligands; D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D001464:Barium; D010739:Phospholipase D; D010450:Endopeptidases; D009327:4-Chloro-7-nitrobenzofurazan; D002118:Calcium; C028694:1-palmitoyl-2-oleoylphosphatidylcholine