Haemoglobin (Erythrogen) enhanced microcallus formation from protoplasts of Indica rice (Oryza sativa L.).
The beneficial effects have been studied of supplementing culture medium with 1:100-1:500 (v:v) of a commercial haemoglobin solution (Erythrogen) on the mitotic division of cell suspension-derived protoplasts of Indica rices (Oryza sativa L.). Protoplasts were cultured in liquid medium, at densities of 1.5 x 10(6) or 2.5 x 10(6) ml(-1), on nitrocellulose membranes overlaying a semi-solidified medium layer that was supplemented with both Erythrogen and nurse cells of Lolium multiflorum. The mean final plating efficiencies (FPEs) of rice cv. BR26 protoplasts cultured with 1:200 (v:v) Erythrogen, at 1.5 x 10(6) ml(-1) (0.018+/-0.003%; n = 8) and 2.5 x 10(6) ml(-1) (0.016+/-0.002%; n = 8), were both significantly (P < 0.05) greater than controls lacking Erythrogen (0.0058+/-0.002%; n = 8 and 0.0041+/-0.001%; n = 8, respectively). Similarly, the mean FPEs of cv. Bini protoplasts cultured with 1:200 (v:v) Erythrogen at 1.5 x 10(6) ml(-1) (0.012+/-0.003%; n = 6) and 2.5 x 10(6) ml(-1) (0.017+/-0.001%; n = 6) were also significantly (P < 0.05) greater than their respective controls (0.003+/-0.001%, n = 6 and 0.002+/-0.001%, n = 6). In contrast, supplementation with 1:100 or 1:500 (v:v) Erythrogen did not lead to sustained mitotic division and microcallus formation in both rice cultivars.
Al-Forkan
M
M
Schools of Life & Environmental Sciences and Biosciences, University of Nottingham, University Park, UK.
Anthony
P
P
Power
J B
JB
Davey
M R
MR
Lowe
K C
KC
D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards; D006454:Hemoglobins / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D012275:Oryza / Q000166:cytology / Q000254:growth & development*; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects*
D006454:Hemoglobins; C446559:erythrogen