[Abnormal augmentation of the evoked potential and morphological changes of guinea pig cochlea induced by cisplatin].
OBJECTIVE
To investigate the characteristic of cochlear microphone(CM), summating potential(SP), compound action potential(CAP) and the morphological changes of hair cells after selective damage to the inner ear by acute cochlear perfusion of cisplatin.


METHODS
Dynamic changes of CM, -SP and CAP were recorded by glass electrode from the third turn of the scala media guinea pig's cochlea before and during cisplatin perfusion of the whole cochlea for 2 hours.


RESULTS
It indicated that after one hour of the perfusion, the amplitude of CM, -SP and CAP decreased at stimulation intensity < or = 60 dB SPL, while the amplitude increased at stimulation intensity > or = 70 dB SPL, as compared with those before perfusion (the average of CM increased by 3.6 mV at 90 dB SPL, average of -SP increased by 1.6 mV at 120 dB SPL, average of CAP increased by 0.23 mV at 90 dB SPL). After two hours of perfusion, the amplitude of CM, -SP and CAP decreased in all the stimulation intensity. The succinic dehydrgenase (SDH) staining was decreased in OHCs, while that of IHC's remained normal. Transmission electron microscopic examination of organ of Corti showed morphological changes in OHCs, such as disappearance of nuclear chromosome, denature and reduction number, mitochondria while the structure of IHCs remained normal.


CONCLUSIONS
The abnormal augmentation phenomenon of CM and -SP may be due to the abnormal modulation of Ca2+ in IHCs and OHCs. The abnormal augmentation of CAP suggests that the suppression effects of OHCs and efferent neurotransmitter on IHCs and afferent neurotransmitter may be reduced after OHCs damage.
Li
X
X
PLA General Hospital, Beijing 100853.
Sun
W
W
Yu
N
N
D000200:Action Potentials; D000818:Animals; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D003055:Cochlear Microphonic Potentials; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000648:ultrastructure; D018072:Hair Cells, Auditory, Outer / Q000201:enzymology; D008297:Male; D009925:Organ of Corti / Q000648:ultrastructure; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D002945:Cisplatin