title: Inhibitory Effects of Polyphenolic Catechins from Chinese Green Tea on HIV Reverse Transcriptase Activity.
abstract: Three polyphenolic catechins, epigallocatechin (1), epicatechin-3-O-gallate (2) and epigallocatechin-3-O-gallate (3), were isolated from Chinese green tea, Ti-Kaun-Yin (Camellia sinensis) and demonstrated as a new class of human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor. The concentrations required for 50% inhibition for the compounds (1), (2) and (3) were 7.80, 0.32 and 0.68 &mgr;M, respectively. The polyphenolic catechins with a galloyl group at the 3 position were potent inhibitors of HIV-RT. Kinetic analysis indicated that the polyphenolic catechins were competitive inhibitors with respect to the template-primer (rA)(n)(dT)(12-18) and noncompetitive inhibitors to dTTP. Copyright 1994 S. Karger AG, Basel
authors.affiliation: Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: C.W.
authors.initials: CW
authors.lastname: Chang
authors.forename: F.L.
authors.initials: FL
authors.lastname: Hsu
authors.forename: J.Y.
authors.initials: JY
authors.lastname: Lin