title: Triacylglycerol-rich lipoproteins interact with human vascular cells in a lipid-dependent fashion.
abstract: Plasma triacylglycerol-rich lipoproteins (TRL) are being considered as a key lipid fraction in the pathogenesis of atherosclerotic cardiovascular disease. Here we compared the influence of two monounsaturated oils [virgin olive oil (VOO) and high-oleic sunflower oil (HOSO)] on the capability of postprandial TRL to interact with two human vascular cell lines [umbilical vein endothelial (HUVEC) and aorta smooth muscle (HASMC) cells]. A fluorescent probe was used for labeling TRL and to determine receptor activity of HUVEC and HASMC. The values for total cell-associated, bound, and internalized TRL were higher in HUVEC, and TRL from VOO was the better ligand recognized but at lower affinity than TRL from HOSO. There was a competitive effect of very low density lipoproteins (VLDL) for the uptake of TRL by cells, which was found to be dependent on the origin/lipid composition of the ligands and cell-type specific. We also conclude that the VLDL receptor (VLDLr) may contribute significantly to the HASMC binding capacity for postprandial TRL mediated by lipoprotein lipase (LPL) or LPL-binding molecules. Our findings are compatible with a selective role of the clustered O-linked sugar domain of the VLDLr in the catabolism of TRL by human vascular cells.
authors.affiliation: Instituto de la Grasa, CSIC, 41012 Sevilla, Spain.
authors.forename: Y M
authors.initials: YM
authors.lastname: Pacheco
authors.forename: R
authors.initials: R
authors.lastname: Abia
authors.forename: J S
authors.initials: JS
authors.lastname: Perona
authors.forename: M
authors.initials: M
authors.lastname: Reina
authors.forename: V
authors.initials: V
authors.lastname: Ruiz-Gutiérrez
authors.forename: E
authors.initials: E
authors.lastname: Montero
authors.forename: F J
authors.initials: FJ
authors.lastname: Muriana
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008297:Male; D011973:Receptors, LDL / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D011973:Receptors, LDL; D014280:Triglycerides; C036053:VLDL receptor