title: [Human interleukin-13 cDNA cloning and expression].
abstract: OBJECTIVE
  To construct an efficient expression system for HIL-13 in prokaryotic cells.
  
  
  METHODS
  After Amplified by RT-PCR, The cDNA fragment encoding HIL-13 was inserted into pGEX-2T plasmid and expressed under induced condition.
  
  
  RESULTS
  HIL-13 cDNA was obtained. Recombinant plasmid pGEX-2T HIL-13 was constructed and sequenced, the inserted fragment of the recombinant plasmid was confirmed to be HIL-13 cDNA. HIL-13 was expressed as a fusion protein with glutathione-s-transferase (GST-HIL-13, MW = 39,000).
  
  
  CONCLUSIONS
  (1) HIL-13 cDNA has been obtained from T lymphocytes. (2) The rate of expression of HIL-13 in prokaryotic cells is about 37%.
authors.affiliation: Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
authors.forename: J
authors.initials: J
authors.lastname: Si
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Song
authors.forename: S
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018793:Interleukin-13 / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D017421:Sequence Analysis
chemical_list: D018076:DNA, Complementary; D018793:Interleukin-13; D011993:Recombinant Fusion Proteins