Human herpes virus 8-negative primary effusion lymphoma in a patient with a ventriculoperitoneal shunt tube.
A 60-year-old woman was referred to our hospital in 1996 due to an abdominal distension in the right lower quadrant. She had undergone a partial resection of a cholesteatoma at the right temporal lobe of the cerebrum 30 years previously, and a ventriculoperitoneal shunt (VPS) tube had been placed with drainage into the right lower peritoneal cavity. The patient developed paralytic ileus in December 1966, and ultrasound and computed tomography of the abdomen revealed a cystic mass in the right lower quadrant without lymphadenopathies or masses. Cytologic examinations of the fluid in the cystic mass revealed signs of malignant lymphoma. After the resection of the cystic mass, lymphoma cells were detected in the fluid, but the wall of the cyst consisted of only fibrous tissues. Results of immunophenotypic analysis of the lymphoma cells by immunocytochemistry or flow cytometry were positive for CD19, CD20, CD22, CD45, and HLA-DR but negative for CD45RO, CD3, CD4, and CD8. The genome of human herpes virus (HHV)-8 was not detected in the lymphoma cells, but Epstein-Barr (EB) nuclear antigen 1 and EB virus (EBV)-encoded small nuclear RNAs were detected. Chromosome analysis by the G-banding method showed complicated abnormalities including der(8)t(2;8)(q31;q24), but Southern blotting analysis suggested that the c-myc oncogene did not participate in the lymphomagenesis. The patient's disease was diagnosed as HHV-8-negative primary effusion lymphoma (PEL). The long-standing inflammatory stimulation by a VPS tube might have contributed to the clonal evolution of EBV-infected lymphocytes. resulting in the development of PEL.
Ashihara
E
E
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan. ash0325@fides.dti.ne.jp
Shimazaki
C
C
Hirai
H
H
Inaba
T
T
Hasegawa
G
G
Mori
S
S
Nakagawa
M
M
D002889:Chromosomes, Human, Pair 2; D002898:Chromosomes, Human, Pair 8; D002999:Clone Cells / Q000473:pathology / Q000821:virology; D020732:Cytogenetic Analysis; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000175:diagnosis; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis; D005260:Female; D019288:Herpesvirus 8, Human; D006801:Humans; D008223:Lymphoma / Q000209:etiology* / Q000473:pathology / Q000821:virology; D008875:Middle Aged; D010534:Peritoneal Neoplasms / Q000209:etiology* / Q000473:pathology / Q000821:virology; D016066:Pleural Effusion, Malignant / Q000209:etiology* / Q000473:pathology / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D014178:Translocation, Genetic; D017287:Ventriculoperitoneal Shunt / Q000009:adverse effects*
D019309:Epstein-Barr Virus Nuclear Antigens; D012367:RNA, Viral; C417029:EBV-encoded nuclear antigen 1