CD5 expression by B lymphocytes and its regulation upon Epstein-Barr virus transformation.
Dim expression of CD5 on human B lymphocytes has been used to delineate B1 and B2 subsets. Nevertheless, others have suggested that the molecule is an activation marker and does not predicate a subset distinction. We have used enzymatic amplification staining, a technology that enhances the resolution of flow cytometric analysis of cell surface molecules by as much as 100-fold, to determine that essentially all human B cells express CD5. Furthermore, we show that this expression is regulated during Epstein-Barr virus transformation.
Kaplan
D
D
Department of Pathology, Case Western Reserve University, Biomedical Research Building, Room 926, 2109 Adelbert Road, Cleveland, OH 44106-4943, USA. drk5@po.cwru.edu
Smith
D
D
Meyerson
H
H
Pecora
N
N
Lewandowska
K
K
D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D018956:CD5 Antigens / Q000096:biosynthesis / Q000235:genetics*; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D014170:Transformation, Genetic
D018956:CD5 Antigens