Anti-lymphoma immunity: relative efficacy of peptide and recombinant DNA vaccine.
The efficacy of vaccines consisting of (1) Ig idiopeptides of a murine B-lymphoblastoma, 2C3, and (2) a corresponding scFV-expressing DNA construct was determined on the basis of their ability to induce anti-tumor immune response and protection against tumor growth. Peptide-KLH complexes and a plasmid expressing single-chain variable fragment (scFv) corresponding to 2C3 Ig VH and VL chains were used separately as vaccines. Immunized BALB/c mice were bled and then challenged with live 2C3 tumor. Survival of various challenged groups was also determined. The results indicate that CDR3-H (VH3) peptides, and the plasmid DNA when given with GM-CSF, only moderately retarded tumor growth, whereas killed 2C3 tumor vaccine induced lasting protection, as shown earlier. Both peptides and scFv-plasmid induced circulating anti-Id antibodies, but no specific cytotoxic T-cell (CTL) activity was detected. However, in spite of their inability to induce CTL activity, both idiopeptides and the scFv-expressing plasmid DNA displayed epitopes recognized by an Id-specific long-term splenic CTL line (A102) obtained from mice vaccinated with killed 2C3 tumor.
Lim
S Y
SY
Department of Life Sciences, Indiana State University, Terre Haute 47809, USA.
Laxmanan
S
S
Stuart
G
G
Ghosh
S K
SK
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D049452:Green Fluorescent Proteins; D007109:Immunity; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology; D008164:Luminescent Proteins / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology*
D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D008164:Luminescent Proteins; D012333:RNA, Messenger; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D049452:Green Fluorescent Proteins