Fluorescent monitoring of kinase activity in real time: development of a robust fluorescence-based assay for Abl tyrosine kinase activity.
Fluorescent biosensors hold great promise for drug discovery. Using a solid-phase version of protein semi-synthesis, we incorporated two fluorophores at specific sites within a truncated version of the c-Crk-II protein. The resulting fluorescent protein biosensor permits the real-time monitoring of Abl kinase activity and provides a robust and rapid method for assaying Abl kinase inhibitors.
Hofmann
R M
RM
Laboratory of Synthetic Protein Chemistry, The Rockefeller University, New York, NY 10021, USA.
Cotton
G J
GJ
Chang
E J
EJ
Vidal
E
E
Veach
D
D
Bornmann
W
W
Muir
T W
TW
D000595:Amino Acid Sequence; D015374:Biosensing Techniques; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016315:Proto-Oncogene Proteins c-abl / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D004791:Enzyme Inhibitors; D016315:Proto-Oncogene Proteins c-abl