title: Immunity induced shortly after DNA vaccination of rainbow trout against rhabdoviruses protects against heterologous virus but not against bacterial pathogens.
abstract: It was recently reported that DNA vaccination of rainbow trout fingerlings against viral hemorrhagic septicaemia virus (VHSV) induced protection within 8 days after intramuscular injection of plasmid DNA. In order to analyse the specificity of this early immunity, fish were vaccinated with plasmid DNA encoding the VHSV or the infectious haematopoietic necrosis virus (IHNV) glycoprotein genes and later challenged with homologous or heterologous pathogens. Challenge experiments revealed that immunity established shortly after vaccination was cross-protective between the two viral pathogens whereas no increased survival was found upon challenge with bacterial pathogens. Within two months after vaccination, the cross-protection disappeared while the specific immunity to homologous virus remained high. The early immunity induced by the DNA vaccines thus appeared to involve short-lived non-specific anti-viral defence mechanisms.
authors.affiliation: Danish Veterinary Laboratory, Hangøvej 2, DK-8200 Aarhus N, Denmark. nl@svs.dk
authors.forename: Niels
authors.initials: N
authors.lastname: Lorenzen
authors.forename: Ellen
authors.initials: E
authors.lastname: Lorenzen
authors.forename: Katja
authors.initials: K
authors.lastname: Einer-Jensen
authors.forename: Scott E
authors.initials: SE
authors.lastname: LaPatra
mesh_terms: D000333:Aeromonas / Q000254:growth & development / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology; D029142:Infectious hematopoietic necrosis virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D016015:Logistic Models; D008969:Molecular Sequence Data; D029141:Novirhabdovirus / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D017686:Oncorhynchus mykiss / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology* / Q000592:standards; D014765:Viral Vaccines / Q000276:immunology; D015007:Yersinia / Q000254:growth & development / Q000276:immunology; D015009:Yersinia Infections / Q000276:immunology / Q000382:microbiology
chemical_list: D019444:Vaccines, DNA; D014765:Viral Vaccines