Induction of the measles virus receptor SLAM (CD150) on monocytes.
Wild-type strains of measles virus (MV) isolated in B95a cells use the signalling lymphocyte activation molecule (SLAM; also known as CD150) as a cellular receptor, whereas the Edmonston strain and its derivative vaccine strains can use both SLAM and the ubiquitously expressed CD46 as receptors. Among the major target cells for MV, lymphocytes and dendritic cells are known to express SLAM after activation, but monocytes have been reported to be SLAM-negative. In this study, SLAM expression on monocytes was examined under different conditions. When freshly isolated from the peripheral blood, monocytes did not express SLAM on the cell surface. However, monocytes became SLAM-positive after incubation with phytohaemagglutinin, bacterial lipopolysaccharide or MV. Anti-SLAM monoclonal antibodies efficiently blocked infection of activated monocytes with a wild-type strain of MV. These results indicate that SLAM is readily induced and acts as a monocyte receptor for MV.
Minagawa
Hiroko
H
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan1.
Tanaka
Kotaro
K
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan1.
Ono
Nobuyuki
N
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan1.
Tatsuo
Hironobu
H
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan1.
Yanagi
Yusuke
Y
Department of Virology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan1.
D015703:Antigens, CD; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000378:metabolism*; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000187:drug effects; D008459:Measles virus / Q000528:radiation effects; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism* / Q000821:virology; D010835:Phytohemagglutinins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014466:Ultraviolet Rays
D015703:Antigens, CD; D006023:Glycoproteins; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1