Cloning of a cDNA encoding an isoform of human protein phosphatase inhibitor 2 from vascularized breast tumor.
Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3'-end RACE and 5'-end RACE. Analysis of the full-length cDNA of this gene reveals that this cDNA encodes an open reading frame of 615 bp, which is highly homologous to human protein phosphatase inhibitor-2, with 92% identity at the nucleotide level, and 89% identity at amino acid level. The results of this study suggest that this novel isoform of human protein phosphatase inhibitor-2 (nPPI-2) may be involved in the angiogenic switch during breast tumor development.
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Laboratory for Surgical Research, Children's Hospital, Department of Surgery, Harvard Medical School, 320 Longwood Ave., Boston, MA 02115, USA.
Moses
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D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009389:Neovascularization, Pathologic; D016366:Open Reading Frames; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D020033:Protein Isoforms / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D000097847:Protein Phosphatase Inhibitory Proteins
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D010749:Phosphoprotein Phosphatases; D020033:Protein Isoforms; D011506:Proteins; C024833:protein phosphatase inhibitor-2; D000097847:Protein Phosphatase Inhibitory Proteins