title: Microfloral diversity of cultured and wild strains of Psoroptes ovis infesting sheep.
abstract: PCR amplification of 16S rDNA was used to determine the diversity of bacteria associated with 3 strains of sheep scab mite, Psoroptes ovis. Eight species of bacteria were identified by phylogenetic analysis of the PCR product sequences. Seven of these species are previously unreported in association with sheep scab mites. Five species were matched to Serratia marcesens, Proponibacteium acnes, Phyllobacterium rubiacearum, Pantoea agglomerans, Curacaobacter baltica, whereas the remaining 3 sequences matched unclassified sequences belonging to the gamma proteobacteria, pseudomonads and streptococci. Bacterial diversity of the in vivo cultured strain was very low and did not match the diversity of the 2 wild collected isolates. The diversity of the bacteria in relation to the disease of sheep scab and the possible importance of these bacteria in the diet of the mites are discussed.
authors.affiliation: School of Biological Sciences, University of Bangor, Gwynedd, Wales. bgyich@leeds.ac.uk
authors.forename: J C
authors.initials: JC
authors.lastname: Hogg
authors.forename: M J
authors.initials: MJ
authors.lastname: Lehane
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008297:Male; D008924:Mite Infestations / Q000662:veterinary; D008925:Mites / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000469:parasitology*; D014966:Xanthenes; D044004:Xanthones
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D014966:Xanthenes; D044004:Xanthones; C025543:psorospermin