[The package and identifying of hNT-3 recombinated retroviral vector].
hNT-3 was inserted into retroviral vector pLXSN to get pLXSN-NT3, and it was transfected into packaging cell line PA317 to form G418 resistant cell clones. The G418 resistant clones were titered and checked for the existence of replication viral. Genome DNA isolated from the cell clone of highest titeration showed the function gene, hNT-3 cDNA, had integrated into the genome of host cells verified by PCR. And bioassay of the clone's cell culture supernant exhibited that it can induce neurite over-growth in the primary culatures of rat spinal cord dorsal root ganglions as compared with controls.
Ouyang
L M
LM
College of Bioengineering, East China University of Science & Technology, Shanghai 200237, China.
Zhou
T
T
Xue
C
C
Liu
Z M
ZM
Zhang
S L
SL
D000818:Animals; D005727:Ganglia, Spinal / Q000378:metabolism; D015316:Genetic Therapy; D005822:Genetic Vectors; D020933:Neurotrophin 3 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D019065:Virus Assembly
D020933:Neurotrophin 3