Molecular characterization of a new adult male putative calycin specific to tergal aphrodisiac secretion in the cockroach Leucophaea maderae.
Lma-p18 is an epicuticular surface protein specific to the tergal gland aphrodisiac secretion of Leucophaea maderae adult males. Native Lma-p18 was purified and the complete cDNA sequence was determined by RT-PCR using primers based on Edman degradation fragments. Northern blot and in situ hybridization analyses showed that Lma-p18 is expressed exclusively in the anterior part of male tergal gland, which is exposed only during sexual behavior. Sequence analysis indicated that Lma-p18 belongs to the calycin superfamily and is very similar to Lma-p22, the first known male-specific tergal protein in L. maderae. Lma-p18 and Lma-p22 were proposed to bind different sexually attractive compounds as other calycins.
Cornette
R
R
Développement-Communication chimique, Unité Mixte de Recherche 5548 du Centre National de la Recherche Scientifique, Université de Bourgogne, 6 Bd Gabriel, 21 000, Dijon, France.
Farine
J P
JP
Quennedey
B
B
Brossut
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003058:Cockroaches / Q000502:physiology*; D005088:Exocrine Glands / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism*; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D019476:Insect Proteins; C442323:p18 protein, Leucophaea maderae