title: Automated trace level determination of glyphosate and aminomethyl phosphonic acid in water by on-line anion-exchange solid-phase extraction followed by cation-exchange liquid chromatography and post-column derivatization.
abstract: An automated method based on the on-line coupling of anion-exchange solid-phase extraction (SPE) and cation-exchange liquid chromatography followed by post-column derivatization and fluorescence detection has been developed for the trace level determination of glyphosate and its primary conversion product aminomethyl phosphonic acid (AMPA) in water. PRP-X100 poly(styrene-divinylbenzene)-trimethylammonium anion-exchange cartridges (20 x 2 mm, 10 microm) were selected for the SPE of glyphosate and AMPA. The ionic compounds present in the samples strongly influenced the extraction of both analytes; however, when an on-line ion-exchange clean-up step was introduced before sample SPE, the problem was largely solved. By processing 100-ml samples detection limits better than 0.02 microg/l for glyphosate and 0.1 microg/l for AMPA were achieved in river water. Both analytes were unstable in solution and the approach of storing samples on the PRP-X100 SPE cartridges was evaluated for a period of 1 month under three different storage conditions (deep freeze, refrigeration and 20 degrees C).
authors.affiliation: Pesticides Science Laboratory, Aristotle University of Thessaloniki, Greece.
authors.forename: J
authors.initials: J
authors.lastname: Patsias
authors.forename: A
authors.initials: A
authors.lastname: Papadopoulou
authors.forename: E
authors.initials: E
authors.lastname: Papadopoulou-Mourkidou
mesh_terms: D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis*; D063065:Organophosphonates / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D000097797:Glyphosate
chemical_list: D002411:Cation Exchange Resins; D005998:Glycine; D063065:Organophosphonates; D014867:Water; D000097797:Glyphosate