[Effects of endogenous TGF-beta during wound healing].
OBJECTIVE
To study the application of exogenous TGF-beta to excision, incision and burn wounds accelerating wound healing.


METHODS
The expression of endogenous TGF-beta during burn wound healing was observed by mRNA dot blot hybridization and immunohistochemistry, respectively.


RESULTS
Thermal injury could induce TGF-beta gene expression in cutaneous wounds. Expression level of TGF-beta mRNA increased over time to reach a peak around day 5 postburn, and declined at day 10 after thermal injury. Immunohistochemistry evidences indicated spatially and temporally that TGF-beta peptide mainly distributed at hair follicles and migrating epidermis at early and middle stages of repair process, and stronger immunoreactivity from days 5 to 10 after wound, which was directly proportional to epidermal cell migration and dermal fibroblast proliferation.


CONCLUSIONS
The expression models of TGF-beta protein and gene suggests that TGF-beta play a critical role in regulation of repair process. Whether the inhibition of expression and overexpression of TGF-beta is correlated to imparied repair and scar formation remains to be studied.
Wei
D
D
Burn Center, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Ge
S
S
Chen
Y
Y
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics; D014945:Wound Healing
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta