Analysis of the cell-dependent replication potentials of human immunodeficiency virus type 1 vif mutants.
Eleven in-frame vif gene mutants of HIV type 1 produced in non-permissive cells were examined for their replication potentials in various CD4-positive and -negative cell lines. Virus replication for each mutant was monitored by using several single- and multiple-cycle infectivity assays. Except for a mutant with wild-type phenotype, most mutants were severely defective for replication in all the cell lines as expected from the producer cell-dependent functioning of Vif so far reported. In contrast, two mutants, which have mutations in the hydrophilic or effector regions of Vif were found to have target cell-dependent replication potentials. These results demonstrate the presence of a novel category of the vif mutants important for elucidation of the Vif function.
Fujita
M
M
Department of Virology, The University of Tokushima School of Medicine, Tokushima-shi, 770-8503, Tokushima, Japan. mfujita@basic.med.tokushima-u.ac.jp
Sakurai
A
A
Doi
N
N
Miyaura
M
M
Yoshida
A
A
Sakai
K
K
Adachi
A
A
D000595:Amino Acid Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D016346:Gene Products, vif / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development*; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D014779:Virus Replication; D054320:vif Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D016346:Gene Products, vif; D054320:vif Gene Products, Human Immunodeficiency Virus