Novel Salmonella enterica serovar Typhimurium protein that is indispensable for virulence and intracellular replication.
Upon contact with host cells, the intracellular pathogen Salmonella enterica serovar Typhimurium promotes its uptake, targeting, and survival in intracellular niches. In this process, the bacterium evades the microbicidal effector mechanisms of the macrophage, including oxygen intermediates. This study reports the phenotypic and genotypic characterization of an S. enterica serovar Typhimurium mutant that is hypersusceptible to superoxide. The susceptible phenotype is due to a MudJ insertion-inactivation of a previously undescribed Salmonella gene designated sspJ that is located between 54.4 and 64 min of the Salmonella chromosome and encodes a 392-amino-acid protein. In vivo, upon intraperitoneal injection of 10(4) to 10(7) bacteria in C3H/HeN and 10(1) to 10(4) bacteria in BALB/c mice, the mutant strain was less virulent than the wild type. Consistent with this finding, during the first hour after ingestion by macrophage-like J774 and RAW264.7 cells in vitro, the intracellular killing of the strain carrying sspJ::MudJ is enhanced fivefold over that of wild-type microorganisms. Wild-type salmonellae displayed significant intracellular replication during the first 24 h after uptake, but sspJ::MudJ mutants failed to do so. This phenotype could be restored to that of the wild type by sspJ complementation. The SspJ protein is found in the cytoplasmic membrane and periplasmic space. Amino acid sequence homology analysis did reveal a leader sequence and putative pyrroloquinoline quinone-binding domains, but no putative protein function. We excluded the possibility that SspJ is a scavenger of superoxide or has superoxide dismutase activity.
van der Straaten
T
T
Department of Infectious Diseases, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
van Diepen
A
A
Kwappenberg
K
K
van Voorden
S
S
Franken
K
K
Janssen
R
R
Kusters
J G
JG
Granger
D L
DL
van Dissel
J T
JT
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D004198:Disease Susceptibility; D024881:Drug Resistance, Bacterial; D005260:Female; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008264:Macrophages / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016254:Mutagenesis, Insertional; D010641:Phenotype; D012481:Salmonella Infections, Animal / Q000401:mortality; D012486:Salmonella typhimurium / Q000187:drug effects / Q000472:pathogenicity*; D013481:Superoxides / Q000494:pharmacology
D001426:Bacterial Proteins; D008565:Membrane Proteins; C440645:sspJ protein, Salmonella; D013481:Superoxides