Impaired transendothelial migration of B-CLL lymphocytes: a defect linked to low L-selectin expression.
The emigration of lymphocytes from blood into lymph nodes is regulated by the expression of the adhesion molecule, L-selectin on the lymphocyte surface which arrests the rolling of the cell on the vessel wall and allows firmer adhesive interactions to develop. The expression of L-selectin on B-CLL lymphocytes is less than half that on normal lymphocytes and this difference correlates with an impaired capacity of B-CLL lymphocytes to migrate beneath a monolayer of human umbilical vein endothelial cells. Both the B-cell and T-cell lymphocytes from normal subjects and B-CLL patients show down-regulation of L-selectin and CD23 after transendothelial migration. The reduced expression of L-selectin on B-CLL lymphocytes leads to a relative "trapping" of these cells in the vascular space and is one factor contributing to the elevation of peripheral lymphocyte count.
Gu
B
B
Sydney University, Department of Medicine, Nepean Hospital, Penrith, NSW 2750, Australia.
Dao
L P
LP
Wiley
J
J
D001402:B-Lymphocytes / Q000473:pathology; D002634:Chemotaxis, Leukocyte; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000503:physiopathology*; D006801:Humans; D019041:L-Selectin / Q000096:biosynthesis / Q000502:physiology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology* / Q000503:physiopathology
D019041:L-Selectin