Construction of a novel display vector deriving from CS3 fimbriae of human enterotoxigenic Escherichia coli.
Based on the prediction of the hydrophilicity, epitopes, secondary structure and flexibility of the CS3 subunit, a novel vector pCSX72 which permits the insertion of foreign epitopes into CS3 at the position of 72nd aa was constructed. Two epitopes, the VP1 of FMDV and a ten-peptides epitope of C-myc, were displayed with it respectively. Compared with the two previously-constructed vectors, the vector pCSX72 expressed the hybrid fimbriae in higher level. Mice produced dual immune response against the CS3 and the inserted epitopes when they were immunized by injecting the live recombinant bacteria intraperitoneally.
Gao
R K
RK
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhang
Z S
ZS
Li
S Q
SQ
Huang
C F
CF
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D000939:Epitopes; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C
D001426:Bacterial Proteins; D000939:Epitopes; D029968:Escherichia coli Proteins; C081391:nfaE protein, E coli; D036781:Fimbriae Proteins