[The transcription of interleukin-8 in gastric epithelial cells by Helicobacter pylori strains in Chinese].
OBJECTIVE
To observe the effect of the vac A genotype and the existence of cag pathogenicity island (cag PAI) of Helicobacter pylori (Hp) isolated from Chinese patients on the transcription of IL-8 in gastric epithelial cells.


METHODS
The genotype of vac A and the existence of cag PAI of Hp were determined by gene sequencing and Southern blot, respectively; the constructed L5F11 cells were cocultured with the bacteria and the transcription of interleukin-8(IL-8) was indicated by luciferase activity and the cytotoxin activity showed by using HeLa cell vacuolating assay.


RESULTS
The ability to induce luciferase by the strains with complete cag PAI (n = 15) was much higher than that by the wild cag negative strain G50[(0.47 +/- 0.09) x 10(6) cpm vs (0.13 +/- 0.02) x 10(6) cpm, P < 0.01] and there was no difference in the luciferase activity induced by the strains with partial cag (n = 4) and by G50[(0.23 +/- 0.08) x 10(6) cpm vs (0.13 +/- 0.02) x 10(6) cpm, P > 0.05]; Also no difference was found in the luciferase activity induced by the strains with vac A s1/m1 (n = 5) and the strains with s1/m2 (n = 14) [(0.29 +/- 0.12) x 10(6) cpm vs (0.53 +/- 0.41) x 10(6) cpm, P > 0.05] although the formers had much higher vacuolating cytotoxin activity than the latters (P < 0.001).


CONCLUSIONS
The transcription of IL-8 in gastric epithelial cells induced by Hp isolated from Chinese patients was associated with the existence of cag PAI and not with the genotype of vac A.
Li
S
S
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Xu
G
G
Ji
X
X
D001426:Bacterial Proteins / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism; D005838:Genotype; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006790:Host-Parasite Interactions; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D013270:Stomach / Q000166:cytology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D016209:Interleukin-8; C074689:VacA protein, Helicobacter pylori