Biomarkers of free radical injury during spinal cord ischemia.
Plasma and urinary levels of 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha) were analysed at baseline and during the ischemia-reperfusion period in experimental spinal cord ischemia. A significant and immediate increase of 8-iso-PGF(2alpha) in plasma at the start and up to 60 min, and in the urine at 90-150 min following ischemia indicate an association of oxidative injury. The inflammatory response indicator 15-keto-dihydro-PGF(2alpha) in plasma increased significantly at the start and up to 60 min after ischemia. No such increase was seen in animals with no spinal cord ischemia. Thus, free radical mediated and cyclooxygenase catalysed products of arachidonic acid are increased during spinal cord ischemia as a consequence of oxidative injury and inflammation.
Basu
S
S
Section of Geriatrics, Faculty of Medicine, Uppsala University, P.O. Box 69, SE-751 25 Uppsala, Sweden. samar.basu@pubcare.uu.se
Hellberg
A
A
Ulus
A T
AT
Westman
J
J
Karacagil
S
S
D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery; D015415:Biomarkers; D002555:Cerebrospinal Fluid / Q000737:chemistry; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood*; D028441:F2-Isoprostanes / Q000097:blood / Q000378:metabolism* / Q000652:urine; D005609:Free Radicals / Q000378:metabolism; D007249:Inflammation / Q000503:physiopathology; D010084:Oxidation-Reduction; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*; D013116:Spinal Cord / Q000503:physiopathology*; D020760:Spinal Cord Ischemia / Q000378:metabolism / Q000503:physiopathology*; D013552:Swine; D013997:Time Factors
D015415:Biomarkers; D028441:F2-Isoprostanes; D005609:Free Radicals; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost