Design and synthesis of water-soluble glycopolymers bearing breast tumor marker and enhanced lipophilicity for solid-phase assays.
Water-soluble T-antigen containing glycopolymers [Gal beta(1,3)-GalNAc alpha) having a high degree of lipophilicity were synthesized from poly[N-(acryloxy)succinimide] (6-10) by amidation with an amine-ending T-antigen derivative (3) and various amines of increasing alkyl chain length (ammonia and methyl-, ethyl-, and propylamine). The enhanced lipophilicity was demonstrated by a solid-phase microtiter plate assay (ELISA) with mouse monoclonal antibody FAA-J11 (IgG3) and by a core 2-beta(1,6)-N-acetylglucoaminyltransferase using tritium-(3H-) labeled UDP-GlcNAc substrate. The new materials were thus useful in solid-phase high-throughput screening for drug discovery.
Baek
M G
MG
Department of Chemistry, University of Ottawa, Ottawa, ON, K1N 6N5, Canada.
Roy
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000957:Antigens, Viral, Tumor / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000737:chemistry*; D001943:Breast Neoplasms / Q000378:metabolism*; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D050091:Dendrimers; D004187:Disaccharides / Q000138:chemical synthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005685:Galactans / Q000138:chemical synthesis*; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids / Q000737:chemistry; D051379:Mice; D011073:Polyamines; D012995:Solubility; D014867:Water
D000911:Antibodies, Monoclonal; D000957:Antigens, Viral, Tumor; D014408:Biomarkers, Tumor; D050091:Dendrimers; D004187:Disaccharides; D005685:Galactans; D007202:Indicators and Reagents; D008055:Lipids; C104700:PAMAM Starburst; D011073:Polyamines; C443716:galactosyll(1,3)-N-acetylgalactosamine; D014867:Water