[Experimental study of LAK and activated NK cells affecting hematopoiesis in mice].
OBJECTIVE
To study the relationship between LAK/activated NK cells and hematopoiesis as well as the effects of LAK/activated NK cells on hematopoietic reconstitution after syngeneic bone marrow transplantation(BMT) in mice.


METHODS
1. LAK/activated NK cells were cocultured with bone marrow cells(BMC) in semi-solid agar medium to determine their effects on CFU-GM formation. 2. Syngeneic LAK/activated NK cells were transferred with BALB/c BMCs into lethally irradiated syngeneic recipients to determine their effects on hematopoietic reconstitution.


RESULTS
When BMC were cultured under optimal growth conditions in the presence of exogenous hematopoietic growth factors, the effects of LAK/activated NK cells were mainly inhibitory. However, when BMCs were cultured under growth-limiting conditions without exogenous hematopoietic growth factors, the LAK/activated NK cells could support CFU-GM formation. Syngeneic LAK/activated NK cells significantly increased CFU-S and CFU-GM numbers after syngeneic BMT, and also significantly improved platelet and white blood cell count.


CONCLUSIONS
LAK/activated NK cells could be bidirectionally regulate CFU-GM formation in vitro and promote hematopoietic reconstitution after syngeneic BMT in mice.
Sun
K
K
Department of Hematology, Affiliated Hospital, Shandong Medical University, Jinan 250012.
Zhang
M
M
Tian
Z
Z
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002478:Cells, Cultured; D018920:Coculture Techniques; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D014185:Transplantation, Isogeneic
D016178:Granulocyte-Macrophage Colony-Stimulating Factor