Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR.
OBJECTIVE
To study the incidence of Shiga-toxigenic Escherichia coli (STEC) in seafoods from India.


RESULTS
Escherichia coli isolated from various seafoods such as fresh fish, clams and water were screened for the presence of stx, hlyA and rfbO157 genes by PCR; 5% of clams and 3% of fresh fish samples were positive for non-O157 STEC.


CONCLUSIONS
STEC is prevalent in seafoods in India, and non-O157 serotype is more common.


CONCLUSIONS
Seafood could be a vehicle for transmission of STEC even in tropical countries.
Sanath Kumar
H
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Department of Fishery Microbiology, College of Fisheries, University of Agricultural Sciences, Mangalore-575 002, India.
Otta
S K
SK
Karunasagar
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I
Karunasagar
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I
D004768:Enterotoxins / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D019453:Escherichia coli O157 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D005516:Food Microbiology; D005798:Genes, Bacterial / Q000235:genetics; D007194:India; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017747:Seafood / Q000382:microbiology*; D022603:Shiga Toxins / Q000032:analysis*; D014774:Virulence
D004768:Enterotoxins; D022603:Shiga Toxins