Fluconazole susceptibility of Italian Candida dubliniensis clinical isolates determined by reference and simplified tests.
Candida dubliniensis ia an opportunistic pathogen mainly associated with oral candidiasis in human immunodeficiency virus (HIV)-infected individuals. We recently recovered the first Italian clinical isolates of C. dubliniensis from the oral cavities of seven HIV-seropositive subjects. The in vitro susceptibility to fluconazole (FLCZ) of these isolates was determined according to the National Committee for Clinical Laboratory Standards (NCCLS) M27-A broth microdilution method for yeasts. All seven isolates of C. dubliniensis were susceptible to FLCZ (MICs < or =0.5 microg/ml). Results of this reference method were compared to those obtained with simplified tests, more adapted to routine evaluation in hospital laboratories. Fungitest and Sensititre YeastOne colorimetric microplate-based methods have been evaluated. The agar disk diffusion method has also been tested on two different media: RPMI 1640-2% glucose and High Resolution-2% glucose-0.5 microg/ml methylene blue. All of the simplified methods tested were able to correctly identify FLCZ-susceptibility of this group of Italian C. dubliniensis isolates.
Giammanco
G M
GM
Dipartimento di Igiene e Microbiologia G. D'Alessandro, Università di Palermo, Italy. gmgiamm@libero.it
Pecorella
S
S
Distefano
S
S
Pecoraro
V
V
Milici
M E
ME
Pizzo
G
G
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology; D003124:Colorimetry / Q000592:standards; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D007558:Italy; D008826:Microbial Sensitivity Tests / Q000592:standards; D008828:Microbiological Techniques / Q000592:standards; D012015:Reference Standards
D000935:Antifungal Agents; D015725:Fluconazole