title: [Cloning, expression and purification of the chicken growth and differentiation factor-8].
abstract: Growth and Differentiation Factor-8(GDF-8) is a new member of TGF-beta super-family. It has been shown that GDF-8 is specifically expressed in skeleton muscle in mouse and its function is to inhibit the growth of muscle cell, so it is named as Myostatin. Here, we amplified 3'half-length GDF-8 cDNA from chicken skeleton muscle by RT-PCR, and cloned it into the prokaryotic expression vector pTrcHisB, which was then transformed into E. coli Top10 cells. The recombinant 6 x His-GDF-8 fusion protein expressed in the Top10 cells was purified by Ni(+)-Affinity Chromatography for future study.
authors.affiliation: Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: W
authors.initials: W
authors.lastname: Yang
authors.forename: H X
authors.initials: HX
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: D H
authors.initials: DH
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002645:Chickens; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018482:Muscle, Skeletal / Q000254:growth & development; D055435:Myostatin; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology
chemical_list: C525912:Mstn protein, mouse; D055435:Myostatin; D011993:Recombinant Fusion Proteins; D016212:Transforming Growth Factor beta