Monoclonal anti-GFAP antibodies: extraction, characteristics, and immunoenzyme assay.
Methods of GFAP purification and obtaining of hybridoma cells producing monoclonal anti-GFAP antibodies and properties of GFAP preparation were described. The immunobloting data on specificity of obtained monoclonal antibodies are presented. A new method of GFAP immunoenzyme assay based on GFAP preparation and anti-GFAP antibodies was elaborated. Standardization of the immunoenzyme system was shown in tests for specificity, accuracy, and reproducibility.
Chekhonin
V P
VP
Laboratory of Immunochemistry, V. P. Serbskii State Research Center of Social and Forensis Psychiatry, Moscow.
Gurina
O I
OI
Dmitrieva
T B
TB
Shepeleva
I I
II
Ryabukhin
I A
IA
Babich
G N
GN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein