title: Severe total hepatic ischemia and reperfusion: relationship between very high alpha-tocopherol uptake and lipid peroxidation.
abstract: Reperfusion injury of the liver occurs in liver transplantation and in major hepatectomies. It triggers a severe oxidative stress that leads to increased lipid peroxidation. In our study we examined the effect of parenteral supranutritional administration of alpha-tocopherol, a vitamin that plays a key role in the endogenous antioxidant system, to rats subjected to severe ischemia/reperfusion (I/R) injury of the liver. alpha-Tocopherol was administered to the animals at doses of 30 and 300 mg/kg bw, whereas total hepatic ischemia was induced for 60 min followed by 120 min reperfusion. Tissue and blood samples were collected for malonyldialdehyde (MDA) and serum alpha-tocopherol assay, respectively. In the sham operation group, mean MDA level in liver was 1.14 nmole/g wet tissue in the control subgroup, and 1.01 or 0.74 nmole/g wet tissue in the subgroups given 30 or 300 mg/kg alpha-tocopherol. In the I/R group, mean MDA level was 1.57 nmole/g wet tissue in the control subgroup, and 0.97 and 0.77 nmole/g wet tissue in the subgroups given 30 or 300 mg/kg alpha-tocopherol. Mean levels of alpha-tocopherol in serum (mumole/l) were 10.20 and 1.80 in the control subgroups, 25.28 and 11.25 in the subgroups treated with 30 and 300 mg/kg bw of alpha-tocopherol, and 31.00 and 13.02 in the subgroups treated with 30 and 300 mg/kg bw of alpha-tocopherol, within the sham-operation and I/R groups, respectively. A significant decrease of MDA accompanied by a significant increase of serum alpha-tocopherol was documented in the alpha-tocopherol-treated rats within both groups. Ischemia/reperfusion triggered a significant increase of the MDA level in the liver of the rats not treated with alpha-tocopherol as compares with the treated animals.
authors.affiliation: Department of Surgery, School of Medical Sciences, Aristotle University, Hospital Hippokration, Thessaloniki, Greece. giakoy@magnet.gr
authors.forename: D
authors.initials: D
authors.lastname: Giakoustidis
authors.forename: N
authors.initials: N
authors.lastname: Kontos
authors.forename: S
authors.initials: S
authors.lastname: Iliadis
authors.forename: G
authors.initials: G
authors.lastname: Papageorgiou
authors.forename: D
authors.initials: D
authors.lastname: Tsantilas
authors.forename: C
authors.initials: C
authors.lastname: Spyridis
authors.forename: K
authors.initials: K
authors.lastname: Papazoglou
authors.forename: N
authors.initials: N
authors.lastname: Botsoglou
authors.forename: A
authors.initials: A
authors.lastname: Dimitriadou
authors.forename: E
authors.initials: E
authors.lastname: Giakoustidis
mesh_terms: D000818:Animals; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D016031:Liver Transplantation / Q000009:adverse effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism*
chemical_list: D008315:Malondialdehyde; D024502:alpha-Tocopherol