Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications.
An efficient system for the analysis of indole alkaloids by HPLC on a reversed-phase column using an ion pair technique is described. The optimised chromatographic conditions allowed the successful separation of 22 standard monoterpenoid indole alkaloids (including some isomers) and tryptamine. The described HPLC system was applied to the analysis of alkaloids in intergeneric somatic hybrid cell cultures of Rauvolfia serpentina x Rhazya stricta.
Gerasimenko
I
I
Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University, Staudinger Weg 5, D-55099 Mainz, Germany.
Sheludko
Y
Y
Unger
M
M
Stöckigt
J
J
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006822:Hybrid Cells; D026121:Indole Alkaloids / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D011926:Rauwolfia / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D026121:Indole Alkaloids; D010936:Plant Extracts