[Different expressions of bFGF gene in three kinds of skeletal muscles].
OBJECTIVE
To explore the distribution characteristics of bFGF gene, bFGF protein and receptor in rat skeletal muscles.


METHODS
bFGF gene, and bFGF protein and receptor in rat gastrocnemius muscles were localized by ABC immunohistochemical method, in situ hybridization and a special staining methods.


RESULTS
Based on the results from the special staining, the fibers in rat gastrocnemius muscle could be divided into three types: type I, II a and II b. The positive expression of bFGF gene was only found in the cytoplasm of type I and type II a fibers. The immunohistochemical localization showed that bFGF protein and its receptor were found in the connective parts of muscle endomysium in fibers of type I and type II a.


CONCLUSIONS
This is the first study to identify the different expressions of bFGF gene, bFGF protein and its receptor in rat skeletal muscles. The results will help to understand the action and the relationship between bFGF autocrine and the embryo development of skeletal muscle fibers.
Fu
X
X
304th Hospital of PLA, Beijing 100037.
Yang
Y
Y
Jiang
L
L
D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis*
D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2