A disposable poly(methylmethacrylate)-based microfluidic module for protein identification by nanoelectrospray ionization-tandem mass spectrometry.
The design, fabrication, and analytical use of a poly(methylmethacrylate) (PMMA)-based microfluidic module for nanoelectrospray ionization-tandem mass spectrometry (nano-ESI-MS/MS) were described. The microfluidic module can be mass-produced at low costs and used as a disposable device to generate nano-ESI-MS/MS signals for protein identification from low amounts of protein samples. Compared with commercially available nanospray capillary tips, the module gave comparable signal quality and also offered advantages in convenience and easiness of operation, permitting repeated usage, and disposability.
Chen
S H
SH
Department of Chemistry, College of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
Sung
W C
WC
Lee
G B
GB
Lin
Z Y
ZY
Chen
P W
PW
Liao
P C
PC
D000818:Animals; D001920:Bradykinin / Q000032:analysis; D002417:Cattle; D004209:Disposable Equipment; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D004867:Equipment Design; D008832:Microchemistry / Q000295:instrumentation*; D008904:Miniaturization; D036103:Nanotechnology / Q000295:instrumentation*; D009842:Oligopeptides / Q000032:analysis; D015675:Osteocalcin / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012212:Rheology; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine / Q000032:analysis; D016622:Silver Staining; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*
D009842:Oligopeptides; D010446:Peptide Fragments; D011506:Proteins; D015675:Osteocalcin; D012710:Serum Albumin, Bovine; D019904:Polymethyl Methacrylate; D001920:Bradykinin