Effects of genotype matching of feline major histocompatibility complex (FLA) class II DRB on skin-allograft transplantation in cats.
In order to confirm the effects of matching of expressed feline major histocompatibility complex (FLA) class II DRB genotype on transplantation immunity in cats, skin-allogeneic transplantation was carried out between cats, in which DRB genes expressed were genotyped by the RT-PCR-RFLP method using group-specific primers. Duration until grafts were rejected was 14.63 +/- 1.69 days (mean +/- standard deviation) in the pairs that had the same type of subgroups, 7.25 +/- 0.71 days in the pairs that had one different type of subgroup and 6.88 +/- 0.35 days in the pairs that had two different types of subgroups. The duration of graft survival in the pairs with the same type of subgroups was significantly longer (P<0.01) than those in the pairs with different types. Although FLA components involved in transplantation immunity should not only be DRB genes, it was suggested that the expressed FLA-DRB genotype might associate with feline transplantation immunity, and that typing and matching of expressed FLA-DRB genes might be one of the important factors in the control of feline transplantation immunity.
Kuwahara
Y
Y
Division of Veterinary Medicine, Faculty of Agriculture, Gifu University, Japan.
Kito
K
K
Kobayash
R
R
Iwata
J
J
Ohne
R
R
Kitagawa
H
H
Sasaki
Y
Y
D000818:Animals; D002415:Cats / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D005802:Genes, MHC Class II / Q000235:genetics / Q000276:immunology*; D005838:Genotype; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016038:Skin Transplantation / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology*
D018076:DNA, Complementary; D012313:RNA