Organization and expression of the Paramecium caudatum gene encoding nucleosome assembly protein 1.
The complete genomic and partial complementary DNAs encoding the ciliate Paramecium caudatum nucleosome assembly protein 1 (NAP1) have been sequenced. The nap1 gene is situated 1.2 kbp from the hemoglobin (hb) gene, with the 3' end of both genes facing each other. The nap1 gene contains no introns, and encodes a protein of 369 amino acid residues with a calculated molecular weight of 42,627. The P. caudatum NAP1 amino acid sequence shares only 23-27% identity with NAP1 amino acid sequences from other eukaryotes. Although the nap1 transcript was detected in the P. caudatum cells at both the logarithmic and stationary phases, its level increased during the stationary phase. Southern blot analysis and polymerase chain reaction amplification revealed that the P. caudatum macronucleus has a heterogeneous composition at genomic regions around the nap1 gene. The present studies indicate the nap1 and hb genes are closely arranged in the macronucleus with the intergenic region between their sequences heterogeneously composed.
Nishiyama
N
N
Department of Biology and Geoscience, Faculty of Science, Shizuoka University, 836 Oya, Shizuoka 422-8529, Japan.
Sawatsubashi
S
S
Ishida
M
M
Yamauchi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D018797:Cell Cycle Proteins; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D017125:Genes, Protozoan / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D010247:Paramecium / Q000235:genetics*; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D016054:DNA, Protozoan; D006454:Hemoglobins; D009687:Nuclear Proteins; D056490:Nucleosome Assembly Protein 1; D011506:Proteins; D012333:RNA, Messenger