title: Rice straw as a support for immobilization of microbial lipase.
abstract: Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2(2) factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.
authors.affiliation: Department of Chemical Engineering and Department of Biotechnology, Faculty of Chemical Engineering of Lorena, P.O. Box 116, 12600-970 Lorena, SP, Brazil. heizir@dequi.faenquil.br
authors.forename: H F
authors.initials: HF
authors.lastname: de Castro
authors.forename: R
authors.initials: R
authors.lastname: de Lima
authors.forename: I C
authors.initials: IC
authors.lastname: Roberto
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D002087:Butyrates / Q000737:chemistry; D002175:Candida / Q000201:enzymology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004952:Esters / Q000737:chemistry; D006868:Hydrolysis; D007202:Indicators and Reagents; D008049:Lipase / Q000737:chemistry*; D000069463:Olive Oil; D012275:Oryza / Q000737:chemistry*; D010938:Plant Oils; D018547:Plant Stems / Q000737:chemistry; D011092:Polyethylene Glycols
chemical_list: D002087:Butyrates; D004800:Enzymes, Immobilized; D004952:Esters; D007202:Indicators and Reagents; D000069463:Olive Oil; D010938:Plant Oils; D011092:Polyethylene Glycols; D020001:1-Butanol; D008049:Lipase