title: Identification of nucleolin as a new L-selectin ligand.
abstract: Apart from leucocyte-endothelial interactions, the adhesion molecule L-selectin mediates the homotypic adhesion of leucocytes during recruitment at sites of acute inflammation, as well as intercellular adhesion of haematopoietic progenitor cells during haematopoiesis. There is evidence that, in addition to P-selectin glycoprotein ligand-1, other as-yet-unidentified proteins function as L-selectin ligands on human leucocytes and haematopoietic progenitor cells. In the present study, we show: (i) by affinity chromatography on L-selectin-agarose; (ii) by protein identification using MS; and (iii) by covalent cell-surface labelling with sulphosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate that the multifunctional nuclear protein nucleolin is partly exposed on the cell surface, and is a ligand of L-selectin in human leucocytes and haematopoietic progenitor cells.
authors.affiliation: Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Hindenburgdamm 30, 12200 Berlin, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Harms
authors.forename: R
authors.initials: R
authors.lastname: Kraft
authors.forename: G
authors.initials: G
authors.lastname: Grelle
authors.forename: B
authors.initials: B
authors.lastname: Volz
authors.forename: J
authors.initials: J
authors.lastname: Dernedde
authors.forename: R
authors.initials: R
authors.lastname: Tauber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019921:Biotinylation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D019041:L-Selectin / Q000378:metabolism*; D008024:Ligands; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D000097796:Nucleolin
chemical_list: D019041:L-Selectin; D008024:Ligands; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin