Up-regulation of the Ire1-mediated signaling molecule, Bip, in ischemic rat brain.
The endoplasmic reticulum (ER) is thought to play important roles in various neurological diseases via multifactorial and complex mechanisms. The Ire1-mediated signal is part of one ER signaling pathways; the signal induces the expression of an ER-resident protein, Bip/GRP78, and is thought to be involved in cell death under ER stress. In this study, we examined time-dependent Bip expression after transient middle cerebral artery occlusion and characterized the Bip-positive cells. Ire1- mediated molecules, Bip, were rapidly up-regulated in the ischemic area after 3.5 h recirculation. Their immunoreactivity continued to increase until 24-48 h. Immunofluorescence staining revealed Bip up-regulation in ischemic neurons, which were TUNEL positive. Our studies suggest that the Ire1-mediated signal might be associated with ischemic neuronal damage.
Ito
D
D
Department of Neurology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Tanaka
K
K
Suzuki
S
S
Dembo
T
T
Kosakai
A
A
Fukuuchi
Y
Y
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D053938:DNA Fragmentation; D004721:Endoplasmic Reticulum / Q000737:chemistry; D000091342:Endoplasmic Reticulum Chaperone BiP; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000502:physiology; D006360:Heat-Shock Proteins; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008565:Membrane Proteins; D018832:Molecular Chaperones / Q000032:analysis / Q000235:genetics*; D009474:Neurons / Q000737:chemistry / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D020521:Stroke / Q000473:pathology / Q000503:physiopathology
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; C486557:Ern2 protein, rat; D017346:Protein Serine-Threonine Kinases