Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis.
A capillary electrophoresis method was developed for the separation and determination of tropane alkaloids in Flos daturae plants. Separation was performed on a fused silica capillary(42.1 cm x 50 microm i.d.) at an applied voltage of 20 kV. Scopolamine, atropine and anisodamine were well separated in the buffer of 50 mmol/L phosphate buffer (pH 5.0) containing 20% (v/v) tetrahydrofuran (THF). Beer's law was obeyed in the range of concentration of 2.4-21.8 microg/mL for scopolamine, 4.0-36.0 microg/mL for atropine and 2.6-23.7 microg/mL for anisodamine, respectively, and the correlation coefficients were over 0.999 (n = 6). The developed method was applied for the analysis of herb samples.
Ye
N
N
Department of Chemistry, Capital Normal University, Beijing 100037, People's Republic of China.
Zhu
R
R
Gu
X
X
Zou
H
H
D001285:Atropine / Q000032:analysis*; D002021:Buffers; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005663:Furans; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis*; D012991:Solanaceous Alkaloids / Q000032:analysis*
D002021:Buffers; D004365:Drugs, Chinese Herbal; D005663:Furans; D012991:Solanaceous Alkaloids; C003922:anisodamine; C018674:tetrahydrofuran; D001285:Atropine; D012601:Scopolamine