title: PEGylated polycyanoacrylate nanoparticles as salvicine carriers: synthesis, preparation, and in vitro characterization.
abstract: OBJECTIVE
  To synthesized poly(methoxypolyethyleneglycol cyanoacrylate-co-n-hexadecyl cyanoacrylate) (PEGylated PHDCA) with polyethylene glycol (PEG, Mr = 5000), prepare PEGylated PHDCA and poly(n-hexadecyl cyanoacrylate) (PHDCA) nanoparticles loading salvicine and determine their in vitro characterizations.
  
  
  METHODS
  The structure of PEGylated PHDCA was determined with 1H-NMR, 13C-NMR and Fourier transform infrared spectrum (FTIR). Its molecular weight was determined by gel permeation chromatography (GPC). Nanoparticles were prepared by emulsion/solvent evaporation method.
  
  
  RESULTS
  1H-NMR, 13C-NMR, and FTIR were consistent with structure of PEGylated PHDCA, whose average molecular weight is 6680. Entrapment efficiency could be determined by high pressure liquid chromatography (HPLC) method without endogenous interference at the retention time of salvicine. The entrapment efficiency was 92.6 % for PEGylated PHDCA nanoparticles and 98.9 % for PHDCA nanoparticles. The nanoparticles size was about 250 nm. The values of the zeta potential were obviously influenced by the composition of the copolymer. Compared with PHDCA nanoparticles (-23.1 mV), PEGylated PHDCA nanoparticles showed a low surface potential (-9.6 mV). Salvicine release from nanoparticles showed an initial burst effect, then a plateau for an extended period, and finally sustained release phase.
  
  
  CONCLUSIONS
  These results showed that the PEGylated PHDCA nanoparticles could be an effective carrier for salvicine delivery in the respect of anti-tumor potency.
authors.affiliation: Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. ypli@mail.shcnc.ac.cn
authors.forename: Y P
authors.initials: YP
authors.lastname: Li
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhou
authors.forename: Y Y
authors.initials: YY
authors.lastname: Pei
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Gu
authors.forename: W F
authors.initials: WF
authors.lastname: Yuan
mesh_terms: D003487:Cyanoacrylates / Q000138:chemical synthesis* / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D036103:Nanotechnology; D009285:Naphthoquinones / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003487:Cyanoacrylates; D004337:Drug Carriers; D009285:Naphthoquinones; C121140:polyethyleneglycol polyhexadecylylcyanoacrylate copolymer; C122096:salvicine; D011092:Polyethylene Glycols