An immunohistochemical study of APG-2 protein in the rat hippocampus after transient forebrain ischemia.
The cellular localization and spatiotemporal expression pattern of APG-2 protein, a member of the heat shock protein 110 family, were investigated in the rat hippocampus after transient forebrain ischemia. The spatiotemporal patterns of immunoreactivity of both APG-2 and glial fibrillary acidic protein were very similar, indicating that reactive astrocytes express APG-2, which was confirmed by double immunofluorescence histochemistry. Colocalization of APG-2 and a neuronal marker NeuN in the neurons of the CA2 and CA3 subfields was also confirmed.
Lee
Mun-Yong
MY
Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea.
Choi
Yun-Sik
YS
Choi
Jeong-Sun
JS
Min
Do Sik
DS
Chun
Myung-Hoon
MH
Kim
Ok Nyu
ON
Lee
Sang Bok
SB
Kim
Seong Yun
SY
D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D050957:HSP110 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis*; D006624:Hippocampus / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D017966:Pyramidal Cells / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D050957:HSP110 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C494655:Hspa4 protein, rat