Analysis of the regulatory region of the heat-shock gene rpoH of Escherichia coli strains isolated from non-human hosts.
The regulatory region of the gene for sigma32, rpoH, of Escherichia coli strains isolated from non-human hosts and different geographic regions, was sequenced and compared with that of E. coli K12. The main nucleotide changes observed are localized to the right inverted octamer motif of the CytR box. The effect of these changes was evaluated using transcriptional fusions. The results presented could guide further studies on the transcription regulation of rpoH using E. coli K12 as a model.
Solís-Guzmán
G
G
Departamento of Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF.
Ramírez-Santos
J
J
Souza
V
V
Gómez-Eichelmann
M C
MC
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D001483:Base Sequence; D001717:Birds / Q000382:microbiology; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D008322:Mammals / Q000382:microbiology; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D017421:Sequence Analysis; D012808:Sigma Factor; D014157:Transcription Factors / Q000235:genetics*
D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32