Copper(II)/H(2)O(2)-mediated DNA cleavage: involvement of a copper(III) species in H-atom abstraction of deoxyribose units.
A new bis-amido-copper(II) complex 2 has been prepared. In the presence of reducing agents (ascorbate or DTT) under air atmosphere or hydrogen peroxide, complex 2 exhibited interesting nuclease activities in the 1-10 microM concentration range. For explaining the activity observed with hydrogen peroxide, we propose the occurrence of a bis-amido-copper(III) intermediate and an oxidation mechanism involving a H-atom abstraction of deoxyribose moieties of DNA.
Amine
Amina
A
Université Moulay Ismail, Faculté des Sciences de Meknès, Meknès, Morocco.
Atmani
Zidane
Z
El Hallaoui
Abdelila
A
Giorgi
Michel
M
Pierrot
Marcel
M
Réglier
Marius
M
D001435:Bacteriophages / Q000235:genetics; D001761:Bleomycin / Q000737:chemistry; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D003855:Deoxyribose / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015195:Drug Design; D006859:Hydrogen; D006861:Hydrogen Peroxide / Q000737:chemistry; D006868:Hydrolysis / Q000187:drug effects; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology; D010084:Oxidation-Reduction; D013329:Structure-Activity Relationship
D009942:Organometallic Compounds; D001761:Bleomycin; D003855:Deoxyribose; D003300:Copper; D006859:Hydrogen; D004247:DNA; D006861:Hydrogen Peroxide