Detection of oxidative species and low-molecular-weight DNA in skin following dermal exposure with JP-8 jet fuel.
Dermal absorption of JP-8 jet fuel can lead to skin irritation within hours after exposure. This study detected the formation of oxidative species and low-molecular-weight DNA in rat skin as potential indicators of JP-8-induced skin injury. At 0, 1, 2, 4 and 6 h after the beginning of a 1-h exposure, skin samples were removed and analyzed for oxidative species formation and low-molecular-weight DNA analysis. At 1, 2 and 4 h, mean oxidative species levels increased significantly (P < 0.05) above unexposed samples. Significantly higher (P < 0.05) low-molecular-weight DNA values were observed at 4 and 6 h compared with unexposed controls. These results demonstrate significant increases in oxidative species and low-molecular-weight DNA levels in the skin following dermal exposure to JP-8. These responses may serve as indicators of skin injury following exposure to JP-8 jet fuel and other volatile chemicals or mixtures.
Rogers
J V
JV
Geo-Centers, Inc., 2856 G. Street (AFRL/HEST), Wright-Patterson Air Force Base, OH 45433, USA.
Gunasekar
P G
PG
Garrett
C M
CM
Kabbur
M B
MB
McDougal
J N
JN
D000042:Absorption; D000279:Administration, Cutaneous; D000401:Aircraft; D000818:Animals; D004249:DNA Damage; D006838:Hydrocarbons / Q000009:adverse effects* / Q000493:pharmacokinetics* / Q000633:toxicity; D007648:Kerosene / Q000009:adverse effects*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000032:analysis*; D012867:Skin / Q000473:pathology; D014835:Volatilization
D006838:Hydrocarbons; D007648:Kerosene; D017382:Reactive Oxygen Species; C029662:JP5 jet fuel