Factor B and the mitochondrial ATP synthase complex.
Factor B is a subunit of the mammalian ATP synthase complex, whose existence has been controversial. This paper describes the molecular and functional properties of a recombinant human factor B, which when added to bovine submitochondrial particles depleted of their factor B restores the energy coupling activity of the ATP synthase complexes. The mature human factor B has 175 amino acids and a molecular mass of 20,341 Da. The preparation is water-soluble, monomeric, and is inactivated by monothiol- and especially dithiol-modifying reagents, probably reacting at its cysteine residues Cys-92 and Cys-94. A likely factor B gene composed of 5 exons has been identified on chromosome 14q21.3, and the functional role of factor B in the mammalian ATP synthase complex has been discussed.
Belogrudov
Grigory I
GI
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.
Hatefi
Youssef
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003545:Cysteine; D004579:Electron Transport / Q000187:drug effects; D005091:Exons; D006801:Humans; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009840:Oligomycins / Q000494:pharmacology; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D009840:Oligomycins; D021122:Protein Subunits; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; C000608097:DMAC2L protein, human; D025261:Mitochondrial Proton-Translocating ATPases; D003545:Cysteine