LPS induces eosinophil migration via CCR3 signaling through a mechanism independent of RANTES and Eotaxin.
Mounting evidence suggests that lipopolysaccharide (LPS) modulates bronchoconstriction and eosinophil function in asthma. We have investigated the role of different chemokines in the eosinophil influx to the pleural cavity after LPS stimulation. Expression of mRNA for eotaxin, regulated on activation, normal T cells expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, MIP-2, and monocyte chemotactic protein (MCP)-1 was increased in cells recovered from the mouse pleural cavity 6 h after LPS administration. Eotaxin and RANTES, but not MIP-1alpha, protein levels were also increased in cell-free pleural washes recovered 6 h after LPS stimulation (LPW). Antimurine eotaxin and antimurine RANTES antibodies (Abs) failed to inhibit LPS-induced eosinophil influx into mouse pleural cavity in vivo. Pertussis toxin inhibited LPW-induced eosinophil shape change in vitro, suggesting the involvement of G protein-coupled receptors in LPW signaling. Blockade of CCR3 receptors diminished eosinophil shape change induced by LPW fractions in vitro and LPS-induced eosinophil accumulation in vivo. To investigate further contribution of CC chemokines, we administered a 35-kD CC chemokine neutralizing protein (vCKBP) in vivo. vCKBP inhibited the eosinophil accumulation induced by eotaxin and ovalbumin, but did not block that induced by LPS or LPW. Our data suggest that LPS-induced eosinophil accumulation depends on G protein-coupled CCR3 receptor activation, through a mechanism independent of eotaxin, RANTES, or other vCKBP-inhibitable CC chemokines.
Penido
C
C
Laboratório de Imunofarmacologia e Laboratório de Inflamação, Departamento de Fisiologia e Farmacodinâmica, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Castro-Faria-Neto
H C
HC
Vieira-de-Abreu
A
A
Figueiredo
R T
RT
Pelled
A
A
Martins
M A
MA
Jose
P J
PJ
Williams
T J
TJ
Bozza
P T
PT
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002352:Carrier Proteins / Q000494:pharmacology; D048429:Cell Size / Q000187:drug effects; D002474:Cell-Free System; D054413:Chemokine CCL11; D018932:Chemokine CCL2 / Q000378:metabolism; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D054426:Chemokine CXCL2; D018925:Chemokines / Q000378:metabolism; D019742:Chemokines, CC / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D004804:Eosinophils / Q000187:drug effects* / Q000502:physiology; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019402:Macrophage Inflammatory Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D037342:Pertussis Toxin; D010994:Pleura / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000502:physiology*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D014764:Viral Proteins / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000906:Antibodies; D002352:Carrier Proteins; C515457:Ccl11 protein, mouse; C515458:Ccl11 protein, rat; C515351:Ccr3 protein, mouse; C515352:Ccr3 protein, rat; D054413:Chemokine CCL11; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054426:Chemokine CXCL2; D018925:Chemokines; D019742:Chemokines, CC; D002630:Chemotactic Factors; C404915:Cxcl2 protein, mouse; D008070:Lipopolysaccharides; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D014764:Viral Proteins; D010566:Virulence Factors, Bordetella; D010047:Ovalbumin; D037342:Pertussis Toxin