title: Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species.
abstract: Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.
authors.affiliation: Department of Microbiology, Lund University, Sölvegatan 12, 22362, Lund, Sweden.
authors.forename: M C
authors.initials: MC
authors.lastname: Gustafsson
authors.forename: C N
authors.initials: CN
authors.lastname: Palmer
authors.forename: C R
authors.initials: CR
authors.lastname: Wolf
authors.forename: C
authors.initials: C
authors.lastname: von Wachenfeldt
mesh_terms: D000222:Adaptation, Physiological; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism* / Q000494:pharmacology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005809:Genes, Regulator; D019787:Linoleic Acid / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000494:pharmacology; D009876:Operon; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D003577:Cytochrome P-450 Enzyme System; D019787:Linoleic Acid; D006899:Mixed Function Oxygenases; C445019:CYP102A3 protein, Bacillus subtilis