title: [Technique of implanting 17 alpha-methyltestosterone to induce the sex reversal of Epinephelus malabaricus].
abstract: This paper discussed the method of implanting 17 alpha-methyltestosterone to induce the sex reversal of Epinephelus malabaricus, which included chemical treatment, dosage and operating on the fish. After twice implanting of exogenous hormone, the mature females of 3 years old were transformed into functional males. Sperm could be squeezed from 58% of the fish on 139 days after operating, and from 100% on 301 days. When the functional males were used for artificial breeding, the parent fish spawned in a natural way, and 71.03 million larvae were hatched in the two years of 1999-2000. The larvae were used for rearing of fish fry, from which normally growing young fish was get. It is believed that the method of implanting exogenous hormone to induce the sex reversal of E. malabaricus is easy and stable, which can satisfy the requirement of artificial breeding in factories.
authors.affiliation: Aquaculture Department of Hainan University, Haikou 570228. chguh@hainu.edu.cn
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: B
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004343:Drug Implants; D005260:Female; D005399:Fishes / Q000502:physiology*; D057850:Hermaphroditic Organisms; D008297:Male; D019849:Sex Determination Processes; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D004343:Drug Implants; D013739:Testosterone