title: Expression pattern of 5'-nucleotidase in Dictyostelium.
abstract: In order to analyze the expression pattern of the 5'-nucleotidase (5nt) gene in Dictyostelium, we made a fusion construct in which the 5nt promoter directed the expression of beta-galactosidase gene. The reporter gene was not active in vegetative amoebae but was expressed during the aggregation stage. At the slug stage, 5nt was highly expressed in pstAB cells. As the slug moved along the substratum, high activity of beta-galactosidase was detected in cells that were left behind in the slime trail. In the completed fruiting body, 5nt was expressed in the lower cup, the anterior like cells (ALC) and the basal disc.
authors.affiliation: Biology Department, Molecular and Cellular Biology Section, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0406, USA.
authors.forename: Muatasem
authors.initials: M
authors.lastname: Ubeidat
authors.forename: Can M
authors.initials: CM
authors.lastname: Eristi
authors.forename: Charles L
authors.initials: CL
authors.lastname: Rutherford
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified; D019677:Artificial Gene Fusion; D004023:Dictyostelium / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017125:Genes, Protozoan; D017930:Genes, Reporter; D007763:Lac Operon; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D015720:5'-Nucleotidase; D001616:beta-Galactosidase