Transformation and regeneration capacities for five Nordic barley elite cultivars--evaluation of tissue culture response and transient expression.
Using both biolistic and Agrobacterium-mediated DNA delivery, we have investigated the transformation and regeneration capacity for five Nordic elite cultivars of barley. Transformation was followed as transient expression of the uidA or gfp gene in barley callus. Callus formation and regeneration of transformed callus were evaluated based on callus induction frequency, growth rate, callus appearance, and shoot formation frequency. From the accumulated results, one of the elite cultivars has been selected for our ongoing work in molecular breeding of barley.
Roussy
I
I
Department of Plant Biology, The Genetic Center, The Swedish University of Agricultural Sciences, Uppsala.
Ahlandsberg
S
S
Jansson
C
C
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D019470:Biolistics / Q000379:methods; D046508:Culture Techniques / Q000379:methods; D015870:Gene Expression; D001467:Hordeum / Q000235:genetics* / Q000502:physiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008027:Light; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development*; D012038:Regeneration; D014170:Transformation, Genetic