title: In vivo and in vitro (27)Al NMR studies of aluminum(III) chelates of triazacyclononane polycarboxylate ligands.
abstract: The metallic radioisotope of a known radiopharmaceutical chelate, (67)Ga(NOTA) (NOTA=1,4,7-triazacyclonane-1,4,7-triacetic acid), used for tumor detection, was substituted by the chemically similar but non radioactive aluminum ion. Our aim was to detect and evaluate the in vivo behavior of the chelate. For this purpose, Al(NOTA) and the related chelate Al(NODASA) (NODASA=1,4,7-triazacyclononane-1-succinic acid-4,7-diacetic acid) were studied using in vitro and in vivo (27)Al NMR spectroscopy in rats. Both chelates showed high stability towards acid catalyzed dissociation and their (27)Al NMR resonances are characteristic of highly symmetrical species, with chemical shifts within the range for octahedral or pseudo-octahedral geometries. The thermodynamic stability constant of the novel chelate Al(NODASA) was estimated using (27)Al NMR. The value obtained suggested that the chelate does not undergo in vivo demetalation by transferrin. The in vivo spectroscopic studies and the analysis of blood and urine samples for Al(III) concentrations indicated that the chelates remain intact under physiological conditions and that they are mainly eliminated from the body through the kidneys.
authors.affiliation: Centro de Química-IBQF, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal. jandre@quimica.uminho.pt
authors.forename: João P
authors.initials: JP
authors.lastname: André
authors.forename: Helmut
authors.initials: H
authors.lastname: Mäcke
authors.forename: Armin
authors.initials: A
authors.lastname: Kaspar
authors.forename: Basil
authors.initials: B
authors.lastname: Künnecke
authors.forename: Margareta
authors.initials: M
authors.lastname: Zehnder
authors.forename: Ludwig
authors.initials: L
authors.lastname: Macko
mesh_terms: D000535:Aluminum / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D004355:Drug Stability; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D006573:Heterocyclic Compounds, 1-Ring; D007554:Isotopes; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D007554:Isotopes; D008024:Ligands; D009942:Organometallic Compounds; C048993:1,4,7-triazacyclononane-N,N',N''-triacetic acid; D000535:Aluminum