title: Impaired type-1 activity and increased NK cells in Gleich's syndrome.
abstract: BACKGROUND
  An altered production of cytokines has been described in Gleich's syndrome. Our aim was to study the cytokine production at the single-cell level in a patient with Gleich's syndrome and to determine whether it changed during a flare episode.
  
  
  METHODS
  Blood samples were obtained from a 30-year-old woman diagnosed with Gleich's syndrome before and during the subsequent flare of edema of trunk and arms and weight gain. The major lymphoid subsets (CD4+, CD8+, and CD19+) and natural killer (NK) cells were measured by flow cytometry. Cytokine-producing T cells (IL-2, IFN-gamma, and IL-4) were quantified in whole blood by intracellular staining with specific monoclonal antibodies and flow cytometry analysis after polyclonal stimulation with phorbol 12-myristate 13-acetate and ionomycin.
  
  
  RESULTS
  Increased numbers of immature CD4+CD8+ T cells and NK cells were observed in peripheral blood during the asymptomatic period. The latter population significantly decreased during the flare. Type-1 cells were decreased in both asymptomatic and, more markedly, during the attack with respect to healthy subjects.
  
  
  CONCLUSIONS
  The decreased type-1 response demonstrated in this patient might be the basis of the hypereosinophilia of Gleich's syndrome. Besides, the NK cells might play a role in the pathogenesis of these inflammatory episodes.
authors.affiliation: Allergy and Immunology Units, Hospital Universitario Marqués de Valdecilla, INSALUD, 39008 Santander, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: García-Abujeta
authors.forename: D
authors.initials: D
authors.lastname: Martín-Gil
authors.forename: M
authors.initials: M
authors.lastname: Martín
authors.forename: R
authors.initials: R
authors.lastname: López
authors.forename: A
authors.initials: A
authors.lastname: Suárez
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Jerez
authors.forename: M
authors.initials: M
authors.lastname: López-Hoyos
mesh_terms: D000328:Adult; D000799:Angioedema / Q000097:blood / Q000276:immunology*; D018941:Antigens, CD19 / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D018655:Lymphocyte Count; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D018941:Antigens, CD19; D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines