Strategies to identify transport systems in plants.
Since the first molecular structures of plant transporters were discovered over a decade ago, considerable advances have been made in the study of plant membrane transport, but we still do not understand transport regulation. The genes encoding the transport systems in the various cell membranes are still to be identified, as are the physiological roles of most transport systems. A wide variety of complementary strategies are now available to study transport systems in plants, including forward and reverse genetics, proteomics, and in silico exploitation of the huge amount of information contained in the completely known genomic sequence of Arabidopsis.
Barbier-Brygoo
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Institut des Sciences du Végétal, UPR 2355, CNRS, 91198 Gif-sur-Yvette Cedex, France. brygoo@isv.cnrs-gif.fr
Gaymard
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F
Rolland
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N
Joyard
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J
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D002736:Chloroplasts / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D008565:Membrane Proteins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification*; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012641:Selection, Genetic
D002352:Carrier Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D020543:Proteome