The effect of anabolic steroid on the distribution of muscle fiber in rat hind limb.
This study evaluates the effect of anabolic steroid hormone (nandrolone decanoate, 19-Nortestosterone; ND) on histomorphometry and distribution of muscle fiber in the rat tibialis anterior (TA) muscle compared with the control rats. Three male Wistar rats aged of 5 weeks were daily received subcutaneous injection of 0.3 cc vehicle oil included 1mg ND for 6 weeks. Two control (C) rats were done only vehicle oil by the same manner as the experimental (E) group. The TA muscle was stained for myofibrillar adenosine triphosphatease with preincubation at pH 4.35, after cryosectioning at the thickness of 10 microm. Muscle fibers were classified into type I, IIA, IIX, and IIB. The E group showed significantly smaller than the C group in body weight. In histomorphometry, the cross-sectional area of type IIB fibers in the E group showed narrow range, and shifted smaller than those in the C groups. The distribution of type I fibers was restricted to the deep region in the E group. The anabolic steroid facilitated toward the older rat type on distribution and proportion of muscular tissue.
Konishi
M
M
Osaka College of Rehabilitation, Department of Occupational Therapy, School of Health Science, Kyushu University of Health and Welfare, Japan. konishi@fukuda.ac.jp
Tokuhara
N
N
Iwamoto
S
S
D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D045930:Anabolic Agents / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D006614:Hindlimb; D006984:Hypertrophy / Q000139:chemically induced*; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D009277:Nandrolone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000077603:Nandrolone Decanoate; D051381:Rats; D017208:Rats, Wistar
D045930:Anabolic Agents; D009277:Nandrolone; D000077603:Nandrolone Decanoate