title: The effects of post-extraction time on the hydraulic conductance of human dentine in vitro.
abstract: The permeability characteristics of dentine have been used in many in vitro studies to evaluate longitudinally the efficacy of various restorative and preventative procedures. However, the permeability of human dentine increases over time after tooth extraction. The aim here was to determine if there is a steady phase in dentine permeability in vitro over time. The convective permeability of dentine discs cut from extracted human third molars to 0.8% albumin solution was measured initially on day 7 after extraction using a fluid-transport model and the hydraulic conductance was calculated. The permeability of each disc was then re-measured de- and re-mounting the split-chamber 1h after the initial measurement as well as on days 8, 9, and 37 after extraction. No significant difference was found between the initial and 1h hydraulic conductances. A significant increase in hydraulic conductance was seen from day 7 to 9, but there was no significant difference between the hydraulic conductances on days 9 and 37. These results suggest that the measurements were reproducible and that a steady phase of dentine permeability is established 9 days after extraction under these study conditions in vitro.
authors.affiliation: Department of Cariology, Endodontology and Pedodontology, Academic Center for Dentistry Amsterdam (ACTA), Louwesweg 1, 1066 EA, Amsterdam, The Netherlands. r.ozok@acta.nl
authors.forename: A R
authors.initials: AR
authors.lastname: Ozok
authors.forename: M K
authors.initials: MK
authors.lastname: Wu
authors.forename: P R
authors.initials: PR
authors.lastname: Wesselink
mesh_terms: D000418:Albumins / Q000493:pharmacokinetics; D003782:Dental Pulp / Q000502:physiology; D003804:Dentin / Q000378:metabolism*; D003806:Dentin Permeability / Q000502:physiology*; D019234:Dentinal Fluid / Q000502:physiology; D015194:Diffusion Chambers, Culture; D006801:Humans; D016555:Matched-Pair Analysis; D008964:Molar, Third; D011312:Pressure; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013997:Time Factors; D014081:Tooth Extraction; D014867:Water / Q000378:metabolism
chemical_list: D000418:Albumins; D014867:Water