Normotopic and heterotopic cortical representations of mystacial vibrissae in rats with subcortical band heterotopia.
The tish rat is a neurological mutant exhibiting bilateral cortical heterotopia similar to those found in certain epileptic patients. Previous work has shown that thalamocortical fibers originating in the ventroposteromedial nucleus, which in normal animals segregate as 'barrel' representations for individual whiskers, terminate in both normotopic and heterotopic areas of the tish cortex (Schottler et al., 1998). Thalamocortical innervation terminates as barrels in layer IV and diffusely in layer VI of the normotopic area. Discrete patches of terminals are also observed in the underlying heterotopic area suggesting that representations of individual vibrissa may be present in the heterotopic somatosensory areas. The present study examines this issue by investigating the organization of the vibrissal somatosensory system in the tish cortex. Staining for cytochrome oxidase or Nissl substance reveals a normal complement of vibrissal barrels in the normotopic area of the tish cortex. Dense patches of cytochrome oxidase staining are also found in the underlying lateral portions of the heterotopic area (i.e. the same area that is innervated by the ventroposteromedial nucleus). Injections of retrograde tracers into vibrissal areas of either the normotopic or heterotopic area produce topographically organized labeling of neurons restricted to one or a small number of barreloids within the ventroposteromedial nucleus of the thalamus. Physical stimulation of a single whisker (D3 or E3) elicits enhanced uptake of [(14)C]2-deoxyglucose in restricted zones of both the normotopic and heterotopic areas, demonstrating that single whisker stimulation can increase functional activity in both normotopic and heterotopic neurons. These findings indicate that the barrels are intact in the normotopic area and are most consistent with the hypothesis that at least some of the individual vibrissae are 'dually' represented in normotopic and heterotopic positions in the primary somatosensory areas of the tish cortex.
Schottler
F
F
Department of Neuroscience, University of Virginia, Box 801392, MR4 Annex, Charlottesville, VA 22098, USA.
Fabiato
H
H
Leland
J M
JM
Chang
L Y
LY
Lotfi
P
P
Getachew
F
F
Lee
K S
KS
D000818:Animals; D019521:Body Patterning / Q000235:genetics; D002828:Choristoma / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D003847:Deoxyglucose / Q000235:genetics; D003576:Electron Transport Complex IV / Q000378:metabolism; D004827:Epilepsy / Q000151:congenital / Q000235:genetics / Q000473:pathology; D005073:Evoked Potentials, Somatosensory / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D009421:Nervous System Malformations / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D009434:Neural Pathways / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009924:Organ Culture Techniques; D051381:Rats; D011922:Rats, Mutant Strains / Q000002:abnormalities* / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology; D020651:Ventral Thalamic Nuclei / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology; D014738:Vibrissae / Q000294:innervation* / Q000502:physiology
D003847:Deoxyglucose; D003576:Electron Transport Complex IV