Mitochondrial AAA-type protease Yme1p is involved in Bax effects on cytochrome c oxidase.
Expression of the pro-apoptotic protein Bax in yeast Saccharomyces cerevisiae induces a release of cytochrome c accompanied by a decrease of the amount of cytochrome c oxidase. Here we show that the decrease of cytochrome c oxidase is due to the activation of mitochondrial protease Yme1p, of which cytochrome c oxidase subunit 2 (Cox2p) is a substrate. The absence of Yme1p slightly delays Bax-induced cell death, suggesting a role of this protease in yeast cell death and thus of its mammalian homologue in apoptosis.
Manon
S
S
UMR5095 C.N.R.S./Université de Bordeaux 2, 1 Rue Camille Saint-Saëns, Bordeaux cedex, F-33077, France. stephen.manon@ibgc.u-bordeaux2.fr
Priault
M
M
Camougrand
N
N
D049069:ATP-Dependent Proteases; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D019069:Cell Respiration; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005800:Genes, Fungal; D016259:Genes, myc; D006801:Humans; D066298:In Vitro Techniques; D008928:Mitochondria / Q000201:enzymology; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D029701:Saccharomyces cerevisiae Proteins; D051028:bcl-2-Associated X Protein; D003576:Electron Transport Complex IV; D049069:ATP-Dependent Proteases; C082702:YME1 protein, S cerevisiae; D000251:Adenosine Triphosphatases