Reporter gene construct containing 1.4-kB alpha 1-proteinase inhibitor promoter confers expression in the cornea of transgenic mice.
A 1.4-kb human alpha1-proteinase inhibitor (alpha1-PI) 5'-flanking sequence fused to the E. coli LacZ gene was used to generate transgenic mice. The 1.4-kb alpha 1-PI fragment was found to target LacZ expression preferentially in the epithelium and stroma of the mouse cornea, and moderately or weakly in white blood cells and a few other tissues, such as the skin and brain. This finding implies that the alpha 1-PI promoter may offer an option for targeting foreign genes in both the epithelial and stromal layers of the cornea in future transgenic experiments.
Ueda
Jun
J
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago College of Medicine, Chicago, Illinois 60612, USA.
Li
Yuhong
Y
Goh
Meilan Stephanie
MS
Maruyama
Yasuhiro
Y
Sugar
Joel
J
Yue
Beatrice Y J T
BY
D000818:Animals; D000831:Animals, Newborn; D003315:Cornea / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D007150:Immunohistochemistry; D007763:Lac Operon / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis* / Q000235:genetics
D017931:DNA Primers; D000515:alpha 1-Antitrypsin; D004247:DNA