title: Toxicity and dosimetry of (177)Lu-DOTA-Y3-octreotate in a rat model.
abstract: Radiolabeled somatostatin analogs have demonstrated effectiveness for targeted radiotherapy of somatostatin receptor-positive tumors in both tumor-bearing rodent models and humans. A radionuclide of interest for cancer therapy is reactor-produced (177)Lu (t(1/2) = 6.64 d; beta(-) [100%]). The high therapeutic efficacy of the somatostatin analog (177)Lu-DOTA-Tyr(3)-octreotate (DOTA-Y3-TATE, where DOTA is 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) was previously demonstrated in a tumor-bearing rat model (Erion et al., J. Nucl. Med. 1999;40:223P; de Jong et al., Int. J. Cancer, 2001; 92:628-633). In the current study, the toxicity and dosimetry of (177)Lu-DOTA-Y3-TATE were determined in both normal and tumor-bearing rats. Doses of (177)Lu-DOTA-Y3-TATE ranging from 0 to 123 mCi/kg were administered to rats and complete blood counts (CBCs) and blood chemistries were analyzed out to 6 weeks. No overt signs of toxicity were observed with (177)Lu-DOTA-Y3-TATE (i.e., lethargy, weight loss, scruffy coat or diarrhea) at any of the dose levels. Blood chemistries and CBCs were normal except for the white blood cell counts, which showed a dose-dependent decrease. The maximum tolerated dose was not reached at 123 mCi/kg. The biodistribution of (177)Lu-DOTA-Y3-TATE was determined in CA20948 rat pancreatic tumor-bearing rats, and the data were used to estimate human absorbed doses to normal tissues. The dose-limiting organ was determined to be the pancreas, followed by the adrenal glands. The absorbed dose to the rat CA20948 tumor was estimated to be 336 rad/mCi (91 mGy/MBq). These data demonstrate that (177)Lu-DOTA-Y3-TATE is an effective targeted radiotherapy agent at levels that show minimal toxicity in this rat model.
authors.affiliation: Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Lewis
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Laforest
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: J L
authors.initials: JL
authors.lastname: Erion
authors.forename: J E
authors.initials: JE
authors.lastname: Bugaj
authors.forename: A
authors.initials: A
authors.lastname: Srinivasan
authors.forename: C J
authors.initials: CJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D008187:Lutetium / Q000633:toxicity*; D008297:Male; D009374:Neoplasms, Experimental / Q000532:radiotherapy*; D015282:Octreotide / Q000031:analogs & derivatives* / Q000633:toxicity*; D019275:Radiopharmaceuticals / Q000633:toxicity*; D011879:Radiotherapy Dosage; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D019275:Radiopharmaceuticals; C121107:octreotide, DOTA-Tyr(3)-; D008187:Lutetium; D015282:Octreotide