Chromosome painting as a tool for rice genetics and breeding.
Chromosome painting and genomic in situ hybridization (GISH) are both effective methods for basic genetic research and practical breeding. These methods were applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.
Shishido
R
R
Department of Plant Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan.
Ohmido
N
N
Fukui
K
K
D001947:Breeding / Q000379:methods*; D020223:Chromosome Painting / Q000379:methods*; D018744:DNA, Plant; D012275:Oryza / Q000235:genetics*; D018626:Retroelements
D018744:DNA, Plant; D018626:Retroelements