Strain-dependent in vitro and in vivo effects of oleic acid anilides on splenocytes and T cells in a murine model of the toxic oil syndrome.
The toxic oil syndrome is an exogenously-induced autoimmune disease in humans, which is believed to be due to the accidental ingestion of oleic acid anilides. In a previously established murine model anilides-treated A/J mice developed a wasting disease after 1 week. Anilides-treated B10.S mice showed after 6 weeks a hyperimmunglobulinemia with autoantibody production, but no clinical symptoms. We now compared in vitro the effects of anilides on splenocytes and T cells in A/J and B10.S mice. Splenocyte proliferation was similar in both strains. After in vivo treatment of mice with anilides and in vitro restimulation, splenocytes of sick A/J mice showed a significant increase in splenocyte proliferation. Splenocytes from B10.S mice, however, had a suppressed baseline response and did not proliferate on restimulation. Adherent cells were necessary to induce proliferation in A/J mice-derived T cells. Apoptosis in splenocytes was significantly elevated in anilides-treated A/J and in B10.S mice as compared to saline-treated controls. These data show that anilides are able to affect the immune system in a strain-dependent way and may therefore take part in inducing the disease seen in humans and mice.
Bell
S A
SA
Department of Dermatology, Ludwig-Maximilians-University Munich, Frauenlobstr. 9-11, D-80337, München, Germany. bellsusanne@hotmail.com
Kuntze
I
I
Caputo
A
A
Chatelain
R
R
D000813:Anilides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001327:Autoimmune Diseases / Q000139:chemically induced*; D002448:Cell Adhesion; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D005229:Fatty Acids, Monounsaturated; D005260:Female; D005506:Food Contamination; D008297:Male; D051379:Mice; D009829:Oleic Acids / Q000494:pharmacology*; D010938:Plant Oils; D000074262:Rapeseed Oil; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D013577:Syndrome; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D000813:Anilides; D005229:Fatty Acids, Monounsaturated; D009829:Oleic Acids; D010938:Plant Oils; D000074262:Rapeseed Oil; C038731:oleoylanilide