Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells.
B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression.
Fischer
G M
GM
Department of Microbiology, Boston University Medical Center, Boston, Massachusetts 02118, USA.
Solt
L A
LA
Hastings
W D
WD
Yang
K
K
Gerstein
R M
RM
Nikolajczyk
B S
BS
Clarke
S H
SH
Rothstein
T L
TL
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000145:classification* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D010537:Peritoneum / Q000166:cytology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D050796:STAT3 Transcription Factor; D013154:Spleen / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015534:Trans-Activators / Q000096:biosynthesis*; D014158:Transcription, Genetic; D000099232:Proto-Oncogene Protein Spi-1
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D016753:Interleukin-10; D008934:Mitogens; D011518:Proto-Oncogene Proteins; D050796:STAT3 Transcription Factor; D013755:Tetradecanoylphorbol Acetate; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C494087:Stat3 protein, mouse