The genome sequence of the facultative intracellular pathogen Brucella melitensis.
Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. The origins of replication of the two chromosomes are similar to those of other alpha-proteobacteria. Housekeeping genes, including those involved in DNA replication, transcription, translation, core metabolism, and cell wall biosynthesis, are distributed on both chromosomes. Type I, II, and III secretion systems are absent, but genes encoding sec-dependent, sec-independent, and flagella-specific type III, type IV, and type V secretion systems as well as adhesins, invasins, and hemolysins were identified. Several features of the B. melitensis genome are similar to those of the symbiotic Sinorhizobium meliloti.
DelVecchio
Vito G
VG
Institute of Molecular Biology and Medicine, University of Scranton, Scranton, PA 18510, USA. vimbm@aol.com
Kapatral
Vinayak
V
Redkar
Rajendra J
RJ
Patra
Guy
G
Mujer
Cesar
C
Los
Tamara
T
Ivanova
Natalia
N
Anderson
Iain
I
Bhattacharyya
Anamitra
A
Lykidis
Athanasios
A
Reznik
Gary
G
Jablonski
Lynn
L
Larsen
Niels
N
D'Souza
Mark
M
Bernal
Axel
A
Mazur
Mikhail
M
Goltsman
Eugene
E
Selkov
Eugene
E
Elzer
Philip H
PH
Hagius
Sue
S
O'Callaghan
David
D
Letesson
Jean-Jacques
JJ
Haselkorn
Robert
R
Kyrpides
Nikos
N
Overbeek
Ross
R
D017347:Brucella melitensis / Q000235:genetics*; D002875:Chromosomes; D005227:Fatty Acids / Q000378:metabolism; D016680:Genome, Bacterial; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D018741:Replication Origin; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
D005227:Fatty Acids