title: Characterization of Toxoplasma gondii-specific T cells recovered from vitreous fluid of patients with ocular toxoplasmosis.
abstract: OBJECTIVE
  The mechanisms involved in reactivations of latent ocular Toxoplasma gondii (Tg) infections in immunocompetent patients are poorly understood. In view of the possible role of T cells in the immunopathogenesis of the disease, ocular infiltrating T cells obtained from patients with recurrent ocular toxoplasmosis were characterized phenotypically and functionally.
  
  
  METHODS
  Ocular infiltrating T cells were recovered from vitreous fluid (VF) samples of 10 patients with active recurrent ocular toxoplasmosis. Two patients with uveitis of other origins were included as control subjects. T-cell lines (TCLs) were generated by mitogenic stimulation and tested for reactivity to Tg and human retinal protein extracts. The TCLs of three patients were cloned by limiting dilution. Tg-reactive T-cell clones (TCCs) were characterized with respect to their phenotype, T-cell receptor variable (TCR V)-beta gene usage, HLA restriction, and cytokine secretion profile.
  
  
  RESULTS
  Reactivity to Tg could be detected only in the TCLs of patients with ocular toxoplasmosis. None of the TCLs showed reactivity to human retinal antigens. All tested intraocular Tg-specific TCCs (n = 23) were CD3+CD4+ and displayed differential TCR Vbeta usage. Twenty-one TCCs were HLA-DR restricted and two TCCs were restricted by HLA-DP. The majority of the intraocular Tg-specific TCCs showed a bias toward a T-helper (Th)0-Th2 cytokine profile.
  
  
  CONCLUSIONS
  The data indicate that T cells specific for the triggering microorganism infiltrate the eye of patients with recurrent ocular toxoplasmosis. The functional characteristics of the VF-derived Tg-specific T cells and their presence at the site of inflammation suggest their involvement in the local inflammatory response of ocular toxoplasmosis.
authors.affiliation: The Rotterdam Eye Hospital, Rotterdam, The Netherlands.
authors.forename: E J
authors.initials: EJ
authors.lastname: Feron
authors.forename: V N
authors.initials: VN
authors.lastname: Klaren
authors.forename: E A
authors.initials: EA
authors.lastname: Wierenga
authors.forename: G M
authors.initials: GM
authors.lastname: Verjans
authors.forename: A
authors.initials: A
authors.lastname: Kijlstra
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D014122:Toxoplasma / Q000276:immunology*; D014126:Toxoplasmosis, Ocular / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D014605:Uveitis / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D014822:Vitreous Body / Q000276:immunology* / Q000473:pathology
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016207:Cytokines; D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta