Effect of six flavonoid compounds from Ixeris sonchifolia on stimulus-induced superoxide generation and tyrosyl phosphorylation in human neutrophils.
BACKGROUND
Ixeris sonchifolia (Bge.) Hance is an herbal medication used in China as an analgesic.


METHODS
The effect of six flavonoid compounds isolated from Ixeris sonchifolia (Bge.) Hance on stimulus-induced superoxide generation and phosphorylation of tyrosine residues of protein in human neutrophils was investigated. The six compounds examined were luteolin 7-glucuronide methylester (LGME), luteolin 7-glucuronide ethylester (LGEE), luteolin 7-glucoside (LG), luteolin 7-glucopyranosyl-(1-->6)-glucoside (LGG6), luteolin 7-glucopyranosyl-(1-->2)-glucoside (LGG2) and apigenin 7-glucoside (AG).


RESULTS
When the cells were preincubated with these six flavonoids, the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was significantly suppressed in a concentration-dependent manner. These flavonoids also suppressed the superoxide generation induced by arachidonic acid (AA). The rate of suppression by these flavonoids was AG>LG, LGG6, LGEE, LGG2>LGME. In case of the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA), LG, LGG6 and AG suppressed the superoxide generation but LGME, LGEE and LGG2 gave no effect. When the cells were incubated with fMLP in the presence of LGME, LGEE and AG, fMLP-induced tyrosyl phosphorylation of 45-kDa proteins of the cells was dose-dependently suppressed in parallel to the suppression of fMLP-induced superoxide generation.


CONCLUSIONS
Flavonoids suppress tyrosine phosphorylase in a dose-dependent manner, and may have pharmacoceutical applications.
Lu
Jincai
J
Department of Pharmacognosy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Shenyang 110015, China.
Feng
Xizhi
X
Sun
Qishi
Q
Lu
Huangwei
H
Manabe
Masanobu
M
Sugahara
Kazunori
K
Ma
Deshun
D
Sagara
Yasuhiro
Y
Kodama
Hiroyuki
H
D047310:Apigenin; D004187:Disaccharides / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology; D020719:Glucuronides / Q000494:pharmacology; D006801:Humans; D047311:Luteolin; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D005419:Flavonoids; D005960:Glucosides; D020719:Glucuronides; C467208:luteolin-7-glucopyranosyl-1-2-glucoside; C467207:luteolin-7-glucopyranosyl-1-6-glucoside; C467206:luteolin-7-glucuronide ethyl ester; C467205:luteolin-7-glucuronide methyl ester; D013481:Superoxides; C075406:luteolin-7-glucuronide; D014443:Tyrosine; D009240:N-Formylmethionine Leucyl-Phenylalanine; C057792:apigetrin; D047310:Apigenin; C066408:luteolin-7-glucoside; D011505:Protein-Tyrosine Kinases; D047311:Luteolin; D013755:Tetradecanoylphorbol Acetate