Construction of mini-Tn5cyaA' and its utilization for the identification of genes encoding surface-exposed and secreted proteins in Bordetella bronchiseptica.
A mini-Tn5 transposon derivative, mini-Tn5cyaA', has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA'). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA' translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.
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Department of Molecular Genetics and Biotechnology, Faculty of Medicine, The Hebrew University, P.O. Box 12272, Jerusalem 91120, Israel.
Nisan
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Miller
J F
JF
Hanski
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E
Rosenshine
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I
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016950:Bordetella bronchiseptica / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins