Microsurgical repair after crush-avulsion injury of the femoral vein in rats: prevention of microvascular thrombosis with recombinant human tissue-type plasminogen activator (rt-PA).
Vein thrombosis is often encountered in microsurgery, especially in the case of crush-avulsion injuries. The aim of this study was to investigate the effect of systemic administration of recombinant tissue-type plasminogen activator (rt-PA) on the patency of the femoral vein of the rat, which had previously sustained a crush-avulsion injury. The study consisted of 3 groups of male Wistar rats, 20 animals each. A standardized crush-avulsion injury model was used. After microvascular repair of the femoral vein, the animals received either normal saline (group A), heparin 100 U/kg body weight (group B), or rt-PA 3.5 mg/kg body weight (group C) systemically. Patency tests were performed at 20 minutes, 48 hours, and 1 week after blood flow reestablishment. According to our results, the patency rate of the rt-PA group was significantly higher than in both the control and heparin groups.
Lykoudis
E G
EG
Microsurgery and Burns Center, Department of Plastic Surgery, General State Hospital of Athens G. Gennimatas, 6 Iktinou Street, 15126 Maroussi, Athens, Greece. elykou@otenet.gr
Contodimos
G B
GB
Tsoutsos
D A
DA
Frangia
K B
KB
Papalois
A E
AE
Stamatopoulos
C N
CN
Ioannovich
J D
JD
D000818:Animals; D005268:Femoral Vein / Q000293:injuries / Q000601:surgery*; D008297:Male; D008866:Microsurgery; D010960:Plasminogen Activators / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*; D014654:Vascular Patency; D020246:Venous Thrombosis / Q000517:prevention & control*
D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator