title: Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extracts.
abstract: A very simple method is proposed to produce, using non-homogeneous hyperthermophilic beta-glycosidase immobilised on chitosan, 3,4-dihydroxy-phenylethanol (hydroxytyrosol), a commercially unavailable compound with well known biological properties which justify a potential commercial application. Leaf extracts from Olea europaea with high oleuropein content are selected as substrate for biotransformation. Under the biotransformation conditions, high amounts of hydroxytyrosol are collected within a short space of time after being preliminarily purified by a non-treated chitosan column. This is possible due to the capacity of amino groups on the chitosan to bind aldehydic groups of molecules present at the end of the reaction. We have produced a natural and non-toxic product from vegetal source, as opposed to the molecule obtainable through chemical synthesis, as a candidate to test in vivo its biological properties. The proposed process may prove useful for a further application for recycling Olea europaea leaves. The radical-scavenging properties of the bioreactor eluates and their capacity to inhibit fatty acid peroxidation rates are characterized in order to make them candidates as substitutes for synthetic antioxidants commonly used to increase the shelf-life of food products as well as for their possible protective effect in human cells.
authors.affiliation: Istituto di Biochimica delle Proteine ed Enzimologia del Consiglio Nazionale delle Ricerche, Via Marconi 10, 80125 Napoli, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Briante
authors.forename: Francesco
authors.initials: F
authors.lastname: La Cara
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Febbraio
authors.forename: Maurizio
authors.initials: M
authors.lastname: Patumi
authors.forename: Roberto
authors.initials: R
authors.lastname: Nucci
mesh_terms: D000975:Antioxidants / Q000032:analysis; D019149:Bioreactors; D001711:Biotransformation; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D057888:Iridoid Glucosides; D039823:Iridoids; D015227:Lipid Peroxidation / Q000187:drug effects; D029592:Oleaceae / Q000378:metabolism*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D011714:Pyrans / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D057888:Iridoid Glucosides; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; C005975:3,4-dihydroxyphenylethanol; C002769:oleuropein; D010626:Phenylethyl Alcohol