Anti-allergen antibodies can be neutralized by antibodies obtained against a peptide complementary to the allergen: towards a new peptide therapy for allergy.
The concept of specific immune treatment against allergic diseases requires the development of antibodies capable of specifically neutralizing anti-allergen antibodies. The aim of the present study was to investigate whether a novel approach, consisting in raising anti-idiotypic blocking antibodies through peptide immunization, could be envisaged in the field of allergy. Using allergy to cow's milk as a model, we prepared polyclonal antibodies against a peptide that is complementary (i.e. hydropathically opposed) to a major epitope of bovine beta-lactoglobulin (BLG), one of the main allergens of bovine milk. Anti-complementary peptide antibodies were found to neutralize in vitro both well-characterized anti-BLG monoclonal antibodies from mice sensitized to BLG and anti-BLG IgE from two patients suffering from milk allergy. These results suggest a new strategy for the functional inhibition of specific disease-associated IgE that may be applicable to the specific treatment of various allergic disorders.
Sélo
Isabelle
I
CEA, Service de Pharmacologie et d'Immunologie, DSV/DRM Ctr d'Etudes de Saclay, Bât. 136, CE Saclay, 91191 Gif-sur-Yvette, Cedex, France.
Créminon
Christophe
C
Grassi
Jacques
J
Couraud
Jean-Yves
JY
D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D019138:Antibodies, Blocking / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007782:Lactoglobulins / Q000276:immunology; D051379:Mice; D016269:Milk Hypersensitivity / Q000188:drug therapy / Q000276:immunology; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use*
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007782:Lactoglobulins; D010455:Peptides; D007073:Immunoglobulin E