Detection of anti-p53 antibodies by ELISA using p53 synthetic or phage-displayed peptides.
Anti-p53 antibodies have been detected in the sera of patients with various types of cancers. In this report, we describe the development of a new ELISA aimed at detecting anti-p53 antibodies using two peptides belonging to immunodominant epitopes of the p53 N-terminal region. We first tested the reactivity of the sera by an indirect ELISA using the peptides as a capture system. Then, the specificity of the reaction was confirmed by an inhibition assay. Two systems of peptide presentation, phage display and the streptavidin/biotin system, were evaluated. Using a panel of sera from cancer patients, both systems were found to be equally reliable, demonstrating that both peptide-based ELISAs can be used for the specific detection of anti-p53 antibodies. The presence of anti-p53 antibodies was associated with p53 alteration whether it be mutation or accumulation.
Portefaix
Jean Michel
JM
CNRS-UMR 5094, Centre de Recherche en Cancérologie, CRLC Val d'Aurelle, Montpellier, France.
Fanutti
Cristina
C
Granier
Claude
C
Crapez
Evelyne
E
Perham
Richard
R
Grenier
Jean
J
Pau
Bernard
B
Del Rio
Maguy
M
D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000097:blood / Q000276:immunology; D014408:Biomarkers, Tumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics; D009154:Mutation; D009369:Neoplasms / Q000097:blood / Q000235:genetics / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D019151:Peptide Library; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D019151:Peptide Library; D016159:Tumor Suppressor Protein p53