title: Knowledge-based system for method development of chiral separations with capillary electrophoresis using highly-sulphated cyclodextrins.
abstract: Method development for chiral separations is not easy because it requires experience and many experimental possibilities can be chosen. In order to help the analyst, a knowledge-based system (KBS) for the rapid determination of experimental parameters, which allow a baseline separation of enantiomers, has been developed. On the basis of own laboratory knowledge, completed with literature data, rules were defined and a KBS was built. Five different techniques are considered in this KBS. This paper describes the capillary electrophoresis (CE) section, in which a strategy has been defined based on the use of highly-sulfated cyclodextrins as chiral selectors. A structured representation of the knowledge and its implementation in Toolbook software is presented.
authors.affiliation: Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit, Laarbeeklaan 103, B-1090 Brussels, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: Matthijs
authors.forename: C
authors.initials: C
authors.lastname: Perrin
authors.forename: M
authors.initials: M
authors.lastname: Maftouh
authors.forename: D L
authors.initials: DL
authors.lastname: Massart
authors.forename: Y
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D001185:Artificial Intelligence; D003505:Cyclodextrins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods; D013237:Stereoisomerism; D013431:Sulfates / Q000032:analysis
chemical_list: D003505:Cyclodextrins; D013431:Sulfates