Palmitate oxidation in rat hepatocytes is inhibited by foetal calf serum.
The rate of oxidation of fatty acids in mammals is minimal prior to birth. In this study, we have shown that foetal calf serum (FCS) inhibits oxidation of palmitate while serum from newborn calves is almost without effect. Foetal calf serum was also found to increase fatty acid synthesis from acetate. Uptake of laurate in mitochondria is partially dependent upon the carnitine palmitoyltransferase (CPT) I/CPT II system, while octanoate transport occurs without its participation. Comparison of the effects of FCS on the oxidation of palmitate, laurate and octanoate supports the view that the observed actions of FCS result from regulation of CPT I activity. The material in FCS that affects fatty acid metabolism has a molecular weight <3 kDa, as determined by dialysis and ultra-filtration studies.
Sleboda
J
J
Institute of Medical Biochemistry, University of Oslo, Oslo, Norway.
Bremer
J
J
Horn
R S
RS
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D004435:Eating; D005215:Fasting; D005326:Fetal Proteins / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010168:Palmitates / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins; D005326:Fetal Proteins; D010168:Palmitates; D002334:Carnitine O-Palmitoyltransferase