Glucose transporter in human glomerular mesangial cells modulated by transforming growth factor-beta and rhein.
OBJECTIVE
To identify GLUT1 in human mesangial cells and its regulation by TGF-beta1 and rhein.


METHODS
Identification of GLUT1 was performed in human mesangial cells by analyzing its mRNA expression, protein product, and functional assay. The effects of TGF-beta1 and rhein on glucose uptake and GLUT1 mRNA expression in mesangial cells were examined by [3H]-2-deoxy-D-glucose uptake and Northern blotting analysis.


RESULTS
It was found that human mesangial cells do express functional GLUT1. The 2-DOG uptake of mesangial cells was markedly increased by TGF-beta1 stimulation with simultaneous elevated expression of GLUT1 mRNA. Increased glucose uptake and GLUT1 mRNA expression in mesangial cells induced by TGF-beta1 were markedly attenuated by rhein in a dose-dependent manner.


CONCLUSIONS
We found that functional GLUT1 did present in human mesangial cells. TGF-beta1 stimulated the glucose uptake in mesangial cells through upregulation of GLUT1 expression, this effect of TGF-beta1 could be antagonized by rhein.
Liu
Z H
ZH
Research Institute of Nephrology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China. zhihong@public1.ptt.js.cn
Li
Y J
YJ
Chen
Z H
ZH
Liu
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D
Li
L S
LS
D000880:Anthraquinones / Q000494:pharmacology*; D002478:Cells, Cultured; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D053773:Transforming Growth Factor beta1
D000880:Anthraquinones; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C020491:rhein