A human RNA polymerase II subunit is encoded by a recently generated multigene family.
BACKGROUND
The sequences encoding the yeast RNA polymerase II (RPB) subunits are single copy genes.


RESULTS
While those characterized so far for the human (h) RPB are also unique, we show that hRPB subunit 11 (hRPB11) is encoded by a multigene family, mapping on chromosome 7 at loci p12, q11.23 and q22. We focused on two members of this family, hRPB11a and hRPB11b: the first encodes subunit hRPB11a, which represents the major RPB11 component of the mammalian RPB complex; the second generates polypeptides hRPB11balpha and hRPB11bbeta through differential splicing of its transcript and shares homologies with components of the hPMS2L multigene family related to genes involved in mismatch-repair functions (MMR). Both hRPB11a and b genes are transcribed in all human tissues tested. Using an inter-species complementation assay, we show that only hRPB11balpha is functional in yeast. In marked contrast, we found that the unique murine homolog of RPB11 gene maps on chromosome 5 (band G), and encodes a single polypeptide which is identical to subunit hRPB11a.


CONCLUSIONS
The type hRPB11b gene appears to result from recent genomic recombination events in the evolution of primates, involving sequence elements related to the MMR apparatus.
Grandemange
S
S
Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS / INSERM / ULP) BP 163, F-67404 ILLKIRCH Cedex, France. sylviegrandemange@hotmail.com
Schaller
S
S
Yamano
S
S
Du Manoir
S
S
Shpakovski
G V
GV
Mattei
M G
MG
Kedinger
C
C
Vigneron
M
M