Uptake of cyclosporine A loaded colloidal drug carriers by mouse peritoneal macrophages in vitro.
OBJECTIVE
To investigate the uptake of cyclosporine A loaded colloidal drug carriers by mouse peritoneal macrophage (MPM) in vitro.


METHODS
The [3H]cyclosporine A loaded colloidal particles: polylactic acid nanospheres, polylactic acid nanocapsules, and microemulsions were prepared. The [3H]cyclosporine A loaded colloidal particles were incubated with MPM for 30 min at 37 degrees C, then the cells were separated from the colloidal particles and the radioactivity was measured by a liquid scintillation counter.


RESULTS
In comparison to the cyclosporine A solution, the binding to polylactic acid nanospheres produced a 20-fold increase in the uptake of cyclosporine A by MPM in 30 min incubation, whereas some obvious decrease in the uptake of cyclosporine A by MPM was observed in the binding of cyclosporine A with polylactic acid nanocapsules or microemulsions. The surfactant coating and plasma protein adsorption were found to have marked effects on the uptake of cyclosporine A loaded nanospheres by MPM.


CONCLUSIONS
Our present study indicated that colloidal drug carriers might affect the targeting of cyclosporine A to mononuclear phagocyte system.
Wang
J
J
Department of Pharmaceutics, School of Pharmaceutical Sciences, Beijing University Health Science Center, Beijing 100083, China.
Zhang
Q
Q
D000818:Animals; D002214:Capsules; D002469:Cell Separation; D016572:Cyclosporine / Q000008:administration & dosage / Q000378:metabolism*; D004337:Drug Carriers; D004655:Emulsions; D005260:Female; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000378:metabolism; D019344:Lactic Acid / Q000008:administration & dosage; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008863:Microspheres; D036103:Nanotechnology; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage
D002214:Capsules; D004337:Drug Carriers; D004655:Emulsions; D007166:Immunosuppressive Agents; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D016572:Cyclosporine