title: Biosensors from conjugated polyelectrolyte complexes.
abstract: A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS(-)), and positively charged methyl viologen (MV(2+)). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.
authors.affiliation: Institute for Polymers and Organic Solids and Materials Department, University of California, Santa Barbara, CA 93106-5090, USA.
authors.forename: Deli
authors.initials: D
authors.lastname: Wang
authors.forename: Xiong
authors.initials: X
authors.lastname: Gong
authors.forename: Peter S
authors.initials: PS
authors.lastname: Heeger
authors.forename: Frauke
authors.initials: F
authors.lastname: Rininsland
authors.forename: Guillermo C
authors.initials: GC
authors.lastname: Bazan
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Heeger
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D015374:Biosensing Techniques; D004573:Electrolytes / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D008027:Light; D008163:Luminescent Measurements; D051379:Mice; D051381:Rats; D012756:Sheep
chemical_list: D001704:Biopolymers; D004573:Electrolytes; D007074:Immunoglobulin G