title: The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein.
abstract: Heterogeneous nuclear ribonucleoprotein K (hnRNP K), a component of hnRNP particles, is involved in several steps of gene expression regulation. Dengue (DEN) virus, a member of the Flaviviridae, is the primary cause of illnesses such as dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. In mature DEN virus particles, the core protein is a structural protein that forms a nucleocapsid complex with genomic RNA. Very little of its biologic functions is known. Here, using an in vitro binding assay and coimmunoprecipitation analysis, we report a protein-protein interaction between the DEN virus core protein and hnRNP K. The C-terminal hydrophilic region of the DEN virus core protein, spanning amino acid residues 73 to 100, is required for such interaction. Results of glutathione-S transferase binding assays indicated that the core protein-hnRNP K interaction might be abolished in the presence of hnRNP K cognate nucleic acids. Furthermore, in a cotransfection experiment, the repressive effect of hnRNP K on C/EBPbeta-mediated transcription activation could be reversed by full-length DEN virus core protein but not by a truncated form containing amino acids 1-72. Our results suggest that, on DEN virus infection, the multiple functions of cellular hnRNP K may be affected by the virus core protein.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. chingjin@gate.sinica.edu.tw
authors.forename: C J
authors.initials: CJ
authors.lastname: Chang
authors.forename: H W
authors.initials: HW
authors.lastname: Luh
authors.forename: S H
authors.initials: SH
authors.lastname: Wang
authors.forename: H J
authors.initials: HJ
authors.lastname: Lin
authors.forename: S C
authors.initials: SC
authors.lastname: Lee
authors.forename: S T
authors.initials: ST
authors.lastname: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D014758:Viral Core Proteins; D012313:RNA