title: A subset of newly synthesized polypeptides in mitochondria from human endothelial cells exposed to hydroperoxide stress.
abstract: The synthesis of 40 polypeptides in mitochondria was found to be stimulated after transient exposure of human endothelial cells to sublethal levels of hydroperoxides, such as H(2)O(2), using comparative two-dimensional polyacrylamide gel electrophoresis. Eleven proteins were identified; these include 60 kDa heat shock protein (HSP60), a mitochondrial type of 70 kDa HSP (mtHSP70), manganese-dependent superoxide dismutase (MnSOD), three metabolic enzymes in citric acid cycle, two components for respiratory chain complexes, a ribosomal protein for translation in mitochondria (RM12), and an unnamed protein. These proteins are involved in reduction-oxidation and protein biogenesis, suggesting that their synthesis, which is triggered under oxidative stress conditions, is aimed at playing a defensive role in mitochondria. Moreover, mtHSP70, HSP60, MnSOD, and RM12 were revealed as their respective precursor proteins with mitochondrial targeting sequences. The preproteins of HSP60 and mtHSP70 were transiently accumulated in mitochondria after the removal of H(2)O(2) in a processing competent state, while the accumulated preprotein of MnSOD localized inside mitochondria and remained unchanged. Membrane potential of mitochondria and cellular ATP levels were unchanged under these conditions. Taken together, these results suggest that hydroperoxide stress leads to preprotein accumulation, possibly due to the impairment of the protein-processing system in mitochondria, independent of membrane potential dissipation and ATP depletion.
authors.affiliation: School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan. mitsumot@helix.mgh.harvard.edu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mitsumoto
authors.forename: Akira
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Katsuya
authors.initials: K
authors.lastname: Okawa
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Nakagawa
mesh_terms: D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D018834:Chaperonin 60 / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010455:Peptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D018834:Chaperonin 60; D010455:Peptides; D011498:Protein Precursors; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase