title: Flavanols and procyanidins of cocoa and chocolate inhibit growth and polyamine biosynthesis of human colonic cancer cells.
abstract: The effects of cocoa powder and extracts with different amounts of flavanols and related procyanidin oligomers were investigated on the growth of Caco-2 cells. Treatment of the cells with 50 microg/ml of procyanidin-enriched (PE) extracts caused a 70% growth inhibition with a blockade of the cell cycle at the G2/M phase. PE extracts caused a significant decrease of ornithine decarboxylase and S-adenosylmethionine decarboxylase activities, two key enzymes of polyamine biosynthesis. This led to a decrease in the intracellular pool of the polyamines. These observations indicate that polyamine metabolism might be an important target in the anti-proliferative effects of cocoa polyphenols.
authors.affiliation: Laboratory of Nutritional Chemoprevention in Digestive Oncology, IRCAD, 1 place de l'hôpital, 67091, Strasbourg, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Carnésecchi
authors.forename: Yann
authors.initials: Y
authors.lastname: Schneider
authors.forename: Sheryl A
authors.initials: SA
authors.lastname: Lazarus
authors.forename: David
authors.initials: D
authors.lastname: Coehlo
authors.forename: Francine
authors.initials: F
authors.lastname: Gossé
authors.forename: Francis
authors.initials: F
authors.lastname: Raul
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids; D002099:Cacao / Q000737:chemistry*; D002392:Catechin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D044945:Proanthocyanidins; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D044946:Biflavonoids; D005419:Flavonoids; D010936:Plant Extracts; D011073:Polyamines; D044945:Proanthocyanidins; C404987:flavan-3-ol; C017674:procyanidin; D002392:Catechin