Two different messenger RNAs for salmon gonadotropin-releasing hormone are expressed in rainbow trout (Oncorhynchus mykiss) brain.
Two different precursor genes encoding the decapeptide salmon GnRH (sGnRH) are present in most salmonid species. In rainbow trout, a precedent Southern blot study revealed the existence of two different sGnRH genes and, recently, two different genes and their complementary DNAs that encode the identical peptide sGnRH were isolated from ovary and testis. Our study confirms the existence of two different mRNAs encoding sGnRH (sGnRH mRNA-I and sGnRH mRNA-II) in the brain of rainbow trout and, for the first time, full-length complementary DNA sequences are given. Central and peripheral distributions of the two messengers are described and seem to indicate different regulation of their expression. sGnRH mRNA-I is found essentially in the olfactory bulbs and telencephalon, whereas sGnRH mRNA-II is more widely expressed in the brain. Our observations allow speculation on the respective roles of two genes encoding the same decapeptide.
Ferriere
F
F
UMR-CNRS 6026, Campus de Beaulieu, 35042 Rennes Cedex, France.
Uzbekova
S
S
Breton
B
B
Jego
P
P
Bailhache
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000235:genetics*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis*; D013687:Telencephalon / Q000737:chemistry
D018076:DNA, Complementary; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone