Synthesis and properties of bacteriorhodopsin analogs containing electron-density labels in the chromophore moiety.
A method for synthesis of retinal analogs labeled with electron-density groups is suggested. The interaction of these polyene compounds with bacterioopsin in apomembrane of Halobacterium salinarum was tested. A retinal analog containing a crown-ether receptor group is able to interact readily with bacterioopsin giving rise to rapid formation of a pigment with absorption maximum at 460 nm. This pigment is capable of undergoing cyclic photoconversion. The crown-bacteriorhodopsin photocycle is extremely slow and its quantum efficiency is very low (approximately 3% of that in native bacteriorhodopsin). This photocycle includes an M-like intermediate with a differential absorption maximum at 380 nm. A retinal analog in which the beta-ionone ring is replaced by ferrocene moiety forms a stable chromoprotein with the main absorption band at 483 nm and a shoulder near 590-610 nm.
Mironova
E V
EV
Lomonosov State Academy of Fine Chemical Technology, Moscow, 117571, Russia. biotechnology@mtu-net.ru
Lukin
A Y
AY
Shevyakov
S V
SV
Alexeeva
S G
SG
Shvets
V I
VI
Demina
O V
OV
Khodonov
A A
AA
Khitrina
L V
LV
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D004583:Electrons; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D001436:Bacteriorhodopsins