title: Binding of LFA-1 (CD11a) to intercellular adhesion molecule 3 (ICAM-3; CD50) and ICAM-2 (CD102) triggers transmigration of human immunodeficiency virus type 1-infected monocytes through mucosal epithelial cells.
abstract: Transmigration of human immunodeficiency virus (HIV)-infected mononuclear cells through the genital mucosa is one of the possible mechanisms of sexual transmission of HIV. Here, we investigated the transmigration of cell-associated R5-tropic HIV type 1 (HIV-1) through a tight monolayer of human epithelial cells in vitro. We show that this process is dependent on an initial interaction between alphaLbeta2 integrin CD11a/CD18 on infected monocytic cells and intercellular adhesion molecule 2 (ICAM-2; CD102) and ICAM-3 (CD50) on the apical membrane of epithelial cells. The CD50 and CD102 ligands were overexpressed on epithelial cells when the cells were activated by proinflammatory cytokines in the cellular microenvironment. An accumulation of proviral DNA was found in the transmigrated cells, clearly reflecting the preferential transepithelial migration of HIV-1-infected cells under proinflammatory conditions. Our observations provide new insights supporting the hypothesis that HIV-infected mononuclear cells contained in genital secretions from infected individuals may cross the epithelial genital mucosa of an exposed receptive sexual partner, particularly under inflammatory conditions of damaged genital tissue. Understanding the fundamental aspects of the initial HIV entry process during sexual transmission remains a critical step for preventing human infection and developing further vaccinal strategies and virucidal agents.
authors.affiliation: Unité 430, Hôpital Broussais, Institut National de la Santé et de la Recherche Médicale. Université Pierre et Marie Curie (Paris VI), Paris, France. marie-paule.carreno@brs.ap-hop-paris.fr
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Carreno
authors.forename: Nicolas
authors.initials: N
authors.lastname: Chomont
authors.forename: Michel D
authors.initials: MD
authors.lastname: Kazatchkine
authors.forename: Theano
authors.initials: T
authors.lastname: Irinopoulou
authors.forename: Corrine
authors.initials: C
authors.lastname: Krief
authors.forename: Ali-Si
authors.initials: AS
authors.lastname: Mohamed
authors.forename: Laurent
authors.initials: L
authors.lastname: Andreoletti
authors.forename: Mathieu
authors.initials: M
authors.lastname: Matta
authors.forename: Laurent
authors.initials: L
authors.lastname: Belec
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D000943:Antigens, Differentiation; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification; D004847:Epithelial Cells / Q000276:immunology; D015497:HIV-1 / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology* / Q000821:virology; D009092:Mucous Membrane / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011533:Proviruses / Q000302:isolation & purification
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D004279:DNA, Viral; C065289:ICAM2 protein, human; C483616:ICAM3 protein, human; D016169:Lymphocyte Function-Associated Antigen-1