title: Dose-dependent behavior of bioactive glass dissolution.
abstract: The effect of glass dosage (0.001 g ml(-1) to 0.015 g ml(-1)) on the in vitro dynamic dissolution behavior of melt-derived 45S5 and sol-gel-derived 58S bioactive glasses, in simulated body fluid (SBF) at 37 degrees C, was evaluated. These glasses differ significantly in texture, especially the specific surface area and porosity, as a result of differences in manufacturing route. The concentrations of elements (Si, Ca, P, and Na) leached from the glasses into the dissolution medium, from 1 to 22 h, were evaluated with the use of induced coupled plasma analysis (ICP). The reacted powders were analyzed with the use of FTIR to observe the formation of a hydroxycarbonate apatite layer on the surface. The results show that the rate of HCA formation on both gel- and melt-derived bioactive glass powders in vitro depends on the concentration of the powders in solution. This result must be taken into account when carrying out in vitro cell-culture studies to simulate conditions in vivo and in experiments using extracts of the bioactive glass powders.
authors.affiliation: Centre for Tissue Regeneration, Department of Materials, Imperial College of Science, Technology and Medicine, Prince Consort Road, SW7 2BP, London, United Kingdom.
authors.forename: J R
authors.initials: JR
authors.lastname: Jones
authors.forename: P
authors.initials: P
authors.lastname: Sepulveda
authors.forename: L L
authors.initials: LL
authors.lastname: Hench
mesh_terms: D001031:Apatites / Q000032:analysis; D001672:Biocompatible Materials / Q000737:chemistry*; D002254:Carbonates / Q000032:analysis; D002516:Ceramics; D004305:Dose-Response Relationship, Drug; D005782:Gels; D005898:Glass / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D016062:Porosity; D011208:Powders; D012995:Solubility; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D002254:Carbonates; D005782:Gels; D011208:Powders; D012996:Solutions; C425330:bioactive glass 45S5; C448994:bioactive glass 58S; C413465:hydroxycarbonate apatite