title: Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound.
abstract: Cofactor F420 is a 5'-deazaflavin derivative first discovered in methanogenic archaea but later found also to be present in some bacteria. As a coenzyme, it is involved in hydride transfer reactions and as a prosthetic group in the DNA photolyase reaction. We report here for the first time on the crystal structure of an F420-dependent oxidoreductase bound with F420. The structure of F420H2:NADP+ oxidoreductase resolved to 1.65 A contains two domains: an N-terminal domain characteristic of a dinucleotide-binding Rossmann fold and a smaller C-terminal domain. The nicotinamide and the deazaflavin part of the two coenzymes are bound in the cleft between the domains such that the Si-faces of both face each other at a distance of 3.1 A, which is optimal for hydride transfer. Comparison of the structures bound with and without substrates reveals that of the two substrates NADP has to bind first, the binding being associated with an induced fit.
authors.affiliation: Max-Planck-Institut für Biophysik, Heinrich-Hoffmann-Strasse 7, D-60596 Frankfurt/Main, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Warkentin
authors.forename: B
authors.initials: B
authors.lastname: Mamat
authors.forename: M
authors.initials: M
authors.lastname: Sordel-Klippert
authors.forename: M
authors.initials: M
authors.lastname: Wicke
authors.forename: R K
authors.initials: RK
authors.lastname: Thauer
authors.forename: M
authors.initials: M
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Iwata
authors.forename: U
authors.initials: U
authors.lastname: Ermler
authors.forename: S
authors.initials: S
authors.lastname: Shima
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D020134:Catalytic Domain; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D019281:Dimerization; D008956:Models, Chemical; D008958:Models, Molecular; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry*; D009249:NADP / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D009249:NADP; C015084:coenzyme F420; D009247:NADH, NADPH Oxidoreductases; C109941:F420-dependent NADP reductase; D004255:Deoxyribodipyrimidine Photo-Lyase; D012256:Riboflavin