Scanning force microscopy at the air-water interface of an air bubble coated with pulmonary surfactant.
To study the structure-function relationship of pulmonary surfactant under conditions close to nature, molecular films of a model system consisting of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylglycerol, and surfactant-associated protein C were prepared at the air-water interface of air bubbles about the size of human alveoli (diameter of 100 microm). The high mechanical stability as well as the absence of substantial film flow, inherent to small air bubbles, allowed for scanning force microscopy (SFM) directly at the air-water interface. The SFM topographical structure was correlated to the local distribution of fluorescent-labeled dipalmitoylphosphatidylcholine, as revealed from fluorescence light microscopy of the same bubbles. Although SFM has proven before to be exceptionally well suited to probe the structure of molecular films of pulmonary surfactant, the films so far had to be transferred onto a solid support from the air-water interface of a film balance, where they had been formed. This made them prone to artifacts imposed by the transfer. Moreover, the supported monolayers disallowed the direct observation of the structural dynamics associated with expansion and compression of the films as upon breathing. The current findings are compared in this respect to our earlier findings from films, transferred onto a solid support.
Knebel
D
D
Institut für Biochemie, Westfälische Wilhelms-Universität, D-48149 Münster, Germany.
Sieber
M
M
Reichelt
R
R
Galla
H-J
HJ
Amrein
M
M
D015060:1,2-Dipalmitoylphosphatidylcholine; D000388:Air; D001896:Boron Compounds; D005456:Fluorescent Dyes; D018625:Microscopy, Atomic Force / Q000379:methods; D010715:Phosphatidylglycerols; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000648:ultrastructure*; D014867:Water
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010715:Phosphatidylglycerols; D011663:Pulmonary Surfactants; D014867:Water; D015060:1,2-Dipalmitoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol