Selective expression of the small GTPase RhoB in the early developing mouse lens.
This report describes the expression and distribution pattern of RhoB GTPase in the developing mouse lens. RhoB expression was confirmed by sequencing an reverse transcriptase-polymerase chain reaction-generated DNA fragment of RhoB. Immunohistochemical analysis of RhoB revealed expression in the lens vesicle (both anterior and posterior vesicle) at embryonic day (E) 11.5, and in the epithelium and primary fibers of the E14.5 lens. Compared with the neonatal stage (day 1), where RhoB is detected in the entire lens (epithelium, primary, and secondary fibers), expression of this protein is restricted to the epithelial and outer cortical secondary fibers in postnatal lenses (from day 7 to day18). Interestingly, in E11.5 and E14.5 lenses, RhoB is localized predominantly in the lens, but not detectable in the retina, cornea, or other ocular tissues. RhoB expression appears to be down-regulated in the postnatal lens with concomitant up-regulation in the retina and cornea, compared with earlier stages of development (eyes of E11.5, E14.5, and neonatal mice). This study reveals the selective expression of RhoB in the lens during early eye development and suggests a potential role for this small GTPase in cytoskeletal reorganization associated with lens epithelial cell elongation and differentiation.
Maddala
R
R
Department of Ophthalmology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Peng
Y W
YW
Rao
P V
PV
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D015153:Blotting, Western; D004622:Embryo, Mammalian / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000196:embryology* / Q000378:metabolism*; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D020743:rhoB GTP-Binding Protein / Q000378:metabolism*
D020743:rhoB GTP-Binding Protein