A Ca(2+)-dependent protein kinase that endows rice plants with cold- and salt-stress tolerance functions in vascular bundles.
A rice Ca(2+)-dependent protein kinase, OsCDPK7, is a positive regulator commonly involved in the tolerance to cold and salt/drought. We carried out in situ detection of the transcript and immunolocalization of the protein. In the wild-type rice plants under both stress conditions, OsCDPK7 was expressed predominantly in vascular tissues of crowns and roots, vascular bundles and central cylinder, respectively, where water stress occurs most severely. This enzyme was also expressed in the peripheral cylinder of crown vascular bundles and root sclerenchyma. Similar localization patterns with stronger signals were observed in stress-tolerant OsCDPK7 over-expressing transformants with the cauliflower mosaic virus 35S promoter. The transcript of a putative target gene of the OsCDPK7 signaling pathway, rab16A, was also detected essentially in the same tissues upon salt stress, suggesting that the OsCDPK7 pathway operates predominantly in these regions. We propose that the use of the 35S promoter fortuitously strengthened the localized expression of OsCDPK7, resulting in enhancement of the stress signaling in the inherently operating regions leading to improved stress tolerance.
Saijo
Y
Y
Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan.
Kinoshita
N
N
Ishiyama
K
K
Hata
S
S
Kyozuka
J
J
Hayakawa
T
T
Nakamura
T
T
Shimamoto
K
K
Yamaya
T
T
Izui
K
K
D000222:Adaptation, Physiological; D002118:Calcium / Q000378:metabolism*; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D007150:Immunohistochemistry; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012492:Salts; D015398:Signal Transduction; D014158:Transcription, Genetic; D014867:Water / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D002118:Calcium; D011494:Protein Kinases; D012492:Salts; D014867:Water; C454865:calcium-dependent protein kinase, rice; D017871:Calcium-Calmodulin-Dependent Protein Kinases