title: Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog.
abstract: We have isolated a full-length cDNA clone that encodes for a Schizosaccharomyces pombe homolog of the dual-specificity protein kinase of the LAMMER family, lkh1 (lammer kinase homolog). The proposed Lkh1 protein contains 575 amino acids. The lkh1(+) null mutant is viable, but exhibits flocculation upon reaching stationary phase in liquid media and filamentous adhesion growth on solid media. Analysis of the flocculation activity of the lkh1(+) null mutant indicates that asexual aggregation of S. pombe cells into floccules is divalent cation-dependent and galactose-specific. We also demonstrate that the Saccharomyces cerevisiae LAMMER kinase homolog, Kns1, can substitute for the Lkh1 function in S. pombe.
authors.affiliation: Department of Microbiology, Chungnam National University, Taejon, 305-764, Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Cho
authors.forename: W H
authors.initials: WH
authors.lastname: Kang
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: K H
authors.initials: KH
authors.lastname: Byun
authors.forename: Y D
authors.initials: YD
authors.lastname: Park
authors.forename: K S
authors.initials: KS
authors.lastname: Bae
authors.forename: H M
authors.initials: HM
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D002448:Cell Adhesion; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005427:Flocculation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology; D017353:Gene Deletion; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D002413:Cations, Divalent; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; C070986:KNS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C446922:lkh1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D005690:Galactose