title: Characterization of PauB, a novel broad-spectrum plasminogen activator from Streptococcus uberis.
abstract: A bovine plasminogen activator of atypical molecular mass ( approximately 45 kDa) from Streptococcus uberis strain SK880 had been identified previously (L. B. Johnsen, K. Poulsen, M. Kilian, and T. E. Petersen. Infect. Immun. 67:1072-1078, 1999). The strain was isolated from a clinical case of bovine mastitis. The isolate was found not to secrete PauA, a bovine plasminogen activator expressed by the majority of S. uberis strains. Analysis of the locus normally occupied by pauA revealed an absence of the pauA open reading frame. However, an alternative open reading frame was identified within the same locus. Sequence analysis of the putative gene suggested limited but significant homology to other plasminogen activators. A candidate signal peptide sequence and cleavage site were also identified. Expression cloning of DNA encoding the predicted mature protein (lacking signal peptide) confirmed that the open reading frame encoded a plasminogen activator of the expected size, which we have named PauB. Both native and recombinant forms of PauB displayed an unexpectedly broad specificity profile for bovine, ovine, equine, caprine, porcine, rabbit, and human plasminogen. Clinical and nonclinical field isolates from nine United Kingdom sites were screened for the pauB gene and none were identified as carrying it. Similarly, clinical isolates from 20 Danish herds were all found to encode PauA and not PauB. Therefore, PauB represents a novel but rare bacterial plasminogen activator which displays very broad specificity.
authors.affiliation: Institute for Animal Health, Compton Laboratory, Compton, Berkshire, RG20 7NN, United Kingdom.
authors.forename: Philip N
authors.initials: PN
authors.lastname: Ward
authors.forename: James A
authors.initials: JA
authors.lastname: Leigh
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D003001:Cloning, Molecular; D003718:Denmark; D005260:Female; D005798:Genes, Bacterial; D008414:Mastitis, Bovine / Q000382:microbiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010960:Plasminogen Activators / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013291:Streptococcus; D013379:Substrate Specificity; D006113:United Kingdom
chemical_list: D001426:Bacterial Proteins; C121687:PauA protein, Streptococcus uberis; C443983:PauB protein, Streptococcus uberis; D010960:Plasminogen Activators