title: Application of terbium-sensitized luminescence for the determination of grepafloxacin in human urine and serum.
abstract: Analytical performance and applications of a selective and sensitive lanthanide-sensitized luminescence method for the determination of grepafloxacin is described. The method is based in the time-resolved luminescence signal from a terbium (III)-grepafloxacin complex in a micellar solution of sodium dodecyl sulfate. The optimized method allows the determination of 10-500 ng mL(-1) of grepafloxacin in 7.5 mM sodium dodecyl sulfate solution containing 0.16 M acetic acid-sodium acetate buffer (pH 6.0) and 7.5 mM Na(2)SO(3) (chemical deoxygenation agent), with lambda(exc) = 275 nm and lambda(em) = 546 nm. Grepafloxacin was determined in human serum (by external calibration method) and urine (by standard additions method), spiked at levels found after drug administration at normal clinical doses. Average recoveries found were 89.6 (relative standard deviation 0.9%) and 102 (relative standard deviation 2%), respectively.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, 41012-Seville, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Ocaña
authors.forename: M
authors.initials: M
authors.lastname: Callejón
authors.forename: F J
authors.initials: FJ
authors.lastname: Barragán
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood* / Q000652:urine*; D024841:Fluoroquinolones; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D010879:Piperazines / Q000097:blood* / Q000652:urine*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry; D013725:Terbium / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D010879:Piperazines; D013501:Surface-Active Agents; D013725:Terbium; D012967:Sodium Dodecyl Sulfate; C075375:grepafloxacin