Sequence variation in the cytochrome oxidase I, internal transcribed spacer 1, and Ts14 diagnostic antigen sequences of Taenia solium isolates from South and Central America, India, and Asia.
We examined the genetic variability in the pig-human tapeworm, Taenia solium, by sequencing the genes for cytochrome oxidase I, internal transcribed spacer 1, and a diagnostic antigen, Ts14, from individual cysts isolated from Peru, Colombia, Mexico, India, China, and the Philippines. For these genes, the rate of nucleotide variation was minimal. Isolates from these countries can be distinguished based on one to eight nucleotide differences in the 396 nucleotide cytochrome oxidase I (COI) sequence. However, all of the 15 isolates from within Peru had identical COI sequences. The Ts14 sequences from India and China were identical and differed from the Peru sequence by three nucleotides in 333. These data indicate that there is minimal genetic variability within the species T. solium. Minimal variability was also seen in the ITS1 sequence, but this variation was observed within the individual. Twenty-two cloned sequences from six isolates sorted into 13 unique sequences. The variability observed within the sequences from individual cysts was as great as the variability between the isolates.
Hancock
K
K
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Bldg 23, Room 1001, Mail Stop F-13, 4770 Buford Highway, Atlanta, GA 30341, USA. khancock@cdc.gov
Broughel
D E
DE
Moura
I N
IN
Khan
A
A
Pieniazek
N J
NJ
Gonzalez
A E
AE
Garcia
H H
HH
Gilman
R H
RH
Tsang
V C
VC
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D002681:China; D003105:Colombia; D018509:DNA, Helminth / Q000235:genetics* / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D007194:India; D008800:Mexico; D008969:Molecular Sequence Data; D010568:Peru; D010679:Philippines; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D013621:Taenia / Q000145:classification / Q000235:genetics*; D013622:Taeniasis / Q000469:parasitology / Q000662:veterinary*
D000947:Antigens, Helminth; D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV