An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine.
In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis of PtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae) fps1(-) mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.
Ciavatta
V T
VT
Institute of Paper Science and Technology, Forest Biology Group, 500 10th Street, Atlanta, Georgia 30318, USA.
Morillon
R
R
Pullman
G S
GS
Chrispeels
M J
MJ
Cairney
J
J
D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D039621:Eukaryotic Initiation Factor-3; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005990:Glycerol / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics; D007473:Ion Channels / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D009865:Oocytes; D009997:Osmotic Pressure; D010802:Phylogeny; D028223:Pinus / Q000235:genetics* / Q000254:growth & development; D041603:Pinus taeda; D010940:Plant Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics; D014982:Xenopus laevis / Q000235:genetics
D020346:Aquaporins; D039621:Eukaryotic Initiation Factor-3; D005656:Fungal Proteins; D017874:Immediate-Early Proteins; D007473:Ion Channels; D008565:Membrane Proteins; C077915:NIP1 protein, S cerevisiae; D009687:Nuclear Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C110065:major intrinsic protein, plant; C077491:tonoplast intrinsic protein, plant; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D005990:Glycerol