title: Paternally inherited transgenes are down-regulated but retain low activity during early embryogenesis in Arabidopsis.
abstract: We investigated the timing of transgene activation after fertilisation in Arabidopsis following crosses and using two transgenic promoters (from the AtCYCB1 and AtLTP1 genes). Using both a transactivation system and direct transcriptional fusion to drive beta-glucuronidase reporter expression, reciprocal crosses showed a lack of expression of the paternal components. This is consistent with a lack of paternal genome activity previously reported during early seed development in Arabidopsis [Viella-Calzada et al. (2000) Nature 404, 91-94]. However, transactivation experiments of the BARNASE gene gave evidence that at least some paternal loci retain transcriptional activity, though at a low level, during early embryogenesis.
authors.affiliation: Génome et Développement des Plantes, Université de Perpignan, 52 avenue de Villeneuve, 66860 Cedex, Perpignan, France.
authors.forename: C
authors.initials: C
authors.lastname: Baroux
authors.forename: R
authors.initials: R
authors.lastname: Blanvillain
authors.forename: P
authors.initials: P
authors.lastname: Gallois
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics*; D001426:Bacterial Proteins; D003433:Crosses, Genetic; D015536:Down-Regulation; D017930:Genes, Reporter; D005966:Glucuronidase / Q000096:biosynthesis; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012260:Ribonucleases / Q000235:genetics; D015533:Transcriptional Activation; D019076:Transgenes
chemical_list: D001426:Bacterial Proteins; D012260:Ribonucleases; C019760:Bacillus amyloliquefaciens ribonuclease; D005966:Glucuronidase