title: VmrA, a member of a novel class of Na(+)-coupled multidrug efflux pumps from Vibrio parahaemolyticus.
abstract: Gene vmrA, cloned from Vibrio parahaemolyticus, made Escherichia coli resistant to 4',6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. VmrA belongs to the DinF branch of MATE family efflux transporters. VmrA catalyzed acriflavine efflux and showed Na(+)/drug transporter activity because the addition of tetraphenylphosphonium to Na(+)-loaded cells caused Na(+) efflux.
authors.affiliation: Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morita
authors.forename: M Nazmul
authors.initials: MN
authors.lastname: Huda
authors.forename: Teruo
authors.initials: T
authors.lastname: Kuroda
authors.forename: Tohru
authors.initials: T
authors.lastname: Mizushima
authors.forename: Tomofusa
authors.initials: T
authors.lastname: Tsuchiya
mesh_terms: D000167:Acriflavine / Q000378:metabolism; D000891:Anti-Infective Agents, Local / Q000378:metabolism; D017920:Antiporters / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D000254:Sodium-Potassium-Exchanging ATPase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D000891:Anti-Infective Agents, Local; D017920:Antiporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; C445308:VmrA protein, Vibrio parahaemolyticus; D000167:Acriflavine; D000254:Sodium-Potassium-Exchanging ATPase