Immunolocalization of osteoclast differentiation factor in rat periodontium.
The aim here was to observe the immunohistochemical localization of osteoclast differentiation factor (ODF)/receptor activator of NF kappa B ligand (RANKL) in the rat periodontium. Rat mandibles were demineralized and embedded in paraffin, and horizontal and frontal sections were prepared for immunohistochemical analysis. In horizontal sections, immunolocalization of RANKL was marked in the distal area of the periodontium of molars in which osteoclasts appeared, due to physiological tooth drift. In frontal sections, RANKL immunoreactivity was localized on spindle-shaped mesenchymal cells around blood vessels near the bone surface in the periodontium. In addition, immunoreaction for RANKL was detected on structures that appeared to be elongated cell processes near blood vessels in frontal sections. Immunohistochemical examination for the general antigen of nerve-specific protein suggested a similarity between these structures and nerve fibres.
Kawamoto
S
S
Second Department of Prosthetic Dentistry, Kagoshima University Dental School, 8-35-1 Sakuragaoka, 890-8544, Kagoshima, Japan. kwmt-s@dentb.hal.kagoshima-u.ac.jp
Ejiri
S
S
Hoshi
K
K
Nagaoka
E
E
Ozawa
H
H
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002352:Carrier Proteins / Q000032:analysis*; D004396:Coloring Agents; D003593:Cytoplasm / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D008024:Ligands; D008334:Mandible / Q000098:blood supply / Q000166:cytology; D008562:Membrane Glycoproteins / Q000032:analysis*; D008648:Mesoderm / Q000166:cytology; D008963:Molar; D016328:NF-kappa B / Q000032:analysis*; D009412:Nerve Fibers / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis; D010010:Osteoclasts / Q000166:cytology*; D010519:Periodontium / Q000098:blood supply / Q000166:cytology*; D053245:RANK Ligand; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D013869:Thiolester Hydrolases / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*; D043222:Ubiquitin Thiolesterase
D015415:Biomarkers; D002352:Carrier Proteins; D004396:Coloring Agents; D007527:Isoenzymes; D008024:Ligands; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha; D013869:Thiolester Hydrolases; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D043222:Ubiquitin Thiolesterase