Cytogenetic and molecular characterization of a patient with simultaneous B-cell chronic lymphocytic leukemia and peripheral T-cell lymphoma.
A patient is described who developed a peripheral T-cell lymphoma (PTCL) after a 6-year history of B-cell chronic lymphocytic leukemia (B-CLL). The progression of the T-cell disease spreading to pleura and skin terminated the course of the disease. A cytogenetic analysis performed six years after the first onset of the B-CLL showed the presence of two clones, one with trisomy 12 and another with inv(14)(q11q32.1) and trisomy 8. Combined immunophenotyping and fluorescence in situ hybridization demonstrated that only CD19+ cells contained a trisomy 12, whereas CD3+ cells contained a trisomy 8. Analyses of IgH and TCR rearrangements in single micromanipulated B- and T-cells lacked evidence for a clonal relation between B-CLL and PTCL cells. Based on our findings, we discuss the different hypotheses which might explain the development of simultaneous PTCL and B-CLL.
Martin-Subero
J I
JI
Institute of Human Genetics, University Hospital Kiel, Germany.
Siebert
R
R
Harder
L
L
Gesk
S
S
Schwindt
P
P
Bräuninger
A
A
Willenbrock
K
K
Steinemann
D
D
Tiemann
M
M
Calasanz
M J
MJ
Grote
W
W
Ludwig
W D
WD
Hansmann
M L
ML
Schlegelberger
B
B
D000368:Aged; D007446:Chromosome Inversion; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020732:Cytogenetic Analysis; D017809:Fatal Outcome; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D016609:Neoplasms, Second Primary / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D014314:Trisomy