Bioanalytical screening methods for dioxins and dioxin-like compounds a review of bioassay/biomarker technology.
Determination of environmental pollutants utilizing biodetectors such as bioassays, biomarkers, enzyme immunoassays (EIAs), or other bioanalytical tools is a continuously growing area. The present literature review describes the principles and advantages/limitations of several bioanalytical detection methods (BDMs) for the screening and diagnosis of dioxin and dioxin-like compounds. This study characterizes briefly the family of dioxin and dioxin-like compounds, discusses potential Ah receptor (AhR) ligands and cytochrome P-450 (CYP) 1A1-enzyme-inducing compounds. 'Milestones' in the development of BDMs are summarized and explained in detail for a number of bioanalytical tools that can be used to detect these classes of dioxin-like persistent bioaccumulative toxicants (PBTs). The design of a screening profile with a battery of bioassays/biomarkers coupled with the chemical analysis is evaluated. The relative potencies (REPs) to 2,3,7,8-TCDD for dioxin-like compounds are reviewed for various BDMs and the differences are noted.
Behnisch
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PA
Life Science Research Laboratories, Kaneka Corporation, Takasago, Japan. behnisch@takaken.kaneka.co.jp
Hosoe
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Sakai
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D000818:Animals; D001681:Biological Assay / Q000379:methods; D015415:Biomarkers / Q000032:analysis; D018929:Cell Culture Techniques; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism*; D004147:Dioxins / Q000009:adverse effects* / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000009:adverse effects / Q000032:analysis; D006801:Humans; D008024:Ligands; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects
D015415:Biomarkers; D004147:Dioxins; D004785:Environmental Pollutants; D008024:Ligands; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D019363:Cytochrome P-450 CYP1A1