Acetylphenylhydrazine induced haemoglobin oxidation in erythrocytes studied by Mössbauer spectroscopy.
The oxidative action of acetylphenylhydrazine (APH) on red blood cells obtained from healthy donors and from patients with breast cancer has been investigated by Mössbauer spectroscopy. Whole blood was incubated with APH for different time periods and the Mössbauer spectra of the packed red cells were recorded and compared. The evolution with time of the oxidation products has been followed. The largest difference in red cells analysis between healthy persons and patients was found after about 50 min of treatment where Mössbauer spectra of patient samples show a much broader spectral pattern due to an advanced haemoglobin oxidation.
Croci
S
S
Instituto di Scienze Fisiche, INFM-Udr Parma, University of Parma, Italy.
Pedrazzi
G
G
Passeri
G
G
Piccolo
P
P
Ortalli
I
I
D001943:Breast Neoplasms / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006427:Hemin / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008706:Methemoglobin / Q000032:analysis; D016877:Oxidants; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000032:analysis; D010659:Phenylhydrazines; D015204:Spectroscopy, Mossbauer; D013997:Time Factors
D006454:Hemoglobins; D016877:Oxidants; D010108:Oxyhemoglobins; D010659:Phenylhydrazines; D006427:Hemin; C100003:deoxyhemoglobin; D008706:Methemoglobin; C006384:N(1)-acetylphenylhydrazine