Multiple splice isoforms of proteolipid M6B in neurons and oligodendrocytes.
Proteolipids are abundant integral membrane proteins, initially described as structural proteins of CNS myelin. More recently, two neuronal proteins related to proteolipid protein (PLP), termed M6A and M6B, were identified, suggesting a common function of proteolipids in oligodendrocytes and neurons. We have analyzed the X-linked M6B gene and discovered an unexpected complexity of protein isoforms. Two promoters and alternative exons yield at least eight M6B proteins and polypeptides, differentially expressed in neurons and oligodendrocytes. Six isoforms are tetraspan membrane proteins that differ by highly conserved amino- and carboxy-terminal domains, termed alpha, beta, psi, and omega. In MDCK cells, the beta-domain of M6B stabilizes tetraspan proteolipids at the cell surface, whereas non-beta isoforms are more abundant in intracellular compartments. Cotransfection experiments suggest a physical interaction of M6B and mutant PLP, when retained in the endoplasmic reticulum, that may also contribute to oligodendrocyte dysfunction in Pelizaeus-Merzbacher disease.
Werner
H
H
Department of Neurogenetics, Max-Planck-Institute of Experimental Medicine, D-37075 Goettingen, Germany.
Dimou
L
L
Klugmann
M
M
Pfeiffer
S
S
Nave
K A
KA
D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D018388:Codon, Terminator / Q000235:genetics; D003600:Cytosol / Q000378:metabolism; D005333:Fetus; D006624:Hippocampus; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D018991:Myelin Proteolipid Protein / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
D018388:Codon, Terminator; C083481:Gpm6b protein, mouse; C484161:Gpm6b protein, rat; D008562:Membrane Glycoproteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger