title: Interaction of Pseudomonas aeruginosa galactophilic lectin PA-IL with pigeon egg white glycoproteins.
abstract: Pseudomonas aeruginosa produces a galactophilic lectin, PA-IL, that resembles P-fimbrial adhesins of uropathogenic Escherichia coli strains in binding to human P blood group antigens. We examined, in the present study, its interaction with pigeon egg white glycoproteins carrying N-glycans with terminal Galalpha1-4Gal which inhibit the adhesion of P-fimbriae. For comparison, the lectin concanavalin A (Con A) and additional avian egg whites (of hen and quail) were also examined. The results obtained in both hemagglutination inhibition and Western blot analyses showed that PA-IL, unlike Con A, preferentially reacted with the pigeon egg white glycoproteins. These results, which confirmed PA-IL similarity in sugar specificity to E. coli P-fimbriae, demonstrated the advantage of this purified lectin for representing P-type and additional galactophilic microbial adhesins unavailable in purified stable form, in Western blot analyses.
authors.affiliation: Faculty of Life Sciences, Bar-Ilan University, 52900, Ramat Gan, Israel.
authors.forename: B
authors.initials: B
authors.lastname: Lerrer
authors.forename: N
authors.initials: N
authors.lastname: Gilboa-Garber
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D010856:Columbidae; D004529:Egg White; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D006023:Glycoproteins; D037102:Lectins