title: Expression of brain subtype creatine kinase in the zebrafish embryo.
abstract: Creatine kinases (CK) play crucial roles in intracellular energy transfer. We have isolated a cDNA from zebrafish embryos, which encodes a CK highly related to the mammalian brain subtype creatine kinase (BCK). The bck mRNA is expressed maternally in the zebrafish embryo and transcripts are distributed uniformly in blastula and gastrula stages. Expression becomes restricted to the prechordal plate and the nervous system during subsequent somitogenesis stages. bck transcripts are abundant in primary neurons in the developing central nervous system of the 1-day-old embryo. While some bck expression persists in the hindbrain, expression vanishes in the spinal cord of the 2-day-old embryo. In summary, the expression pattern of bck is highly dynamic and suggests a role for bck during gastrulation and neuronal differentiation.
authors.affiliation: Institut de Génétique et de Biologie, Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, F-67404 Illkirch Cedex, C.U. de Strasbourg, France.
authors.forename: T
authors.initials: T
authors.lastname: Dickmeis
authors.forename: S
authors.initials: S
authors.lastname: Rastegar
authors.forename: P
authors.initials: P
authors.lastname: Aanstad
authors.forename: M
authors.initials: M
authors.lastname: Clark
authors.forename: N
authors.initials: N
authors.lastname: Fischer
authors.forename: C
authors.initials: C
authors.lastname: Plessy
authors.forename: F
authors.initials: F
authors.lastname: Rosa
authors.forename: V
authors.initials: V
authors.lastname: Korzh
authors.forename: U
authors.initials: U
authors.lastname: Strähle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation; D002490:Central Nervous System / Q000196:embryology; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000096:biosynthesis*; D052276:Creatine Kinase, BB Form; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology; D013997:Time Factors; D015027:Zebrafish
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D003402:Creatine Kinase; D052276:Creatine Kinase, BB Form