title: Subjects with mild and moderate asthma respond to segmental allergen challenge with similar, reproducible, allergen-specific inflammation.
abstract: BACKGROUND
  The relationship between severity of asthma and bronchial inflammation is poorly understood.
  
  
  OBJECTIVE
  We examined acute and subacute inflammatory responses to allergen in subjects with mild and moderate persistent asthma to evaluate whether different cellular and mediator responses to endobronchial allergen challenge are associated with differences in disease severity.
  
  
  METHODS
  Segmental allergen challenge was performed in 8 subjects with mild and 10 subjects with moderate allergic asthma to compare baseline airways inflammation and allergen-induced inflammatory responses 24 hours later. This evaluation was repeated after 6 weeks in 9 subjects to investigate the reproducibility of these inflammatory responses.
  
  
  RESULTS
  Subjects with mild and moderate asthma had similar decreases in FEV(1) in response to segmental allergen challenge (9.1% +/- 4.2% vs 15.1% +/- 4.6%, P = .35). There was no difference in inflammatory cell counts or cytokine concentrations in the groups with mild and moderate asthma at baseline or after saline or allergen challenge. Repeat segmental allergen challenge 6 weeks later showed that these cellular and cytokine responses were reproducible.
  
  
  CONCLUSIONS
  Segmental allergen challenge in subjects with mild and moderate asthma produces similar allergen-specific physiologic and inflammatory responses that are reproducible 6 weeks later. In this model of allergic asthma, acute responses to allergen do not appear to be related to disease severity.
authors.affiliation: Center for Human Genomics and the Section on Pulmonary and Critical Care Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Moore
authors.forename: J D
authors.initials: JD
authors.lastname: Hasday
authors.forename: S S
authors.initials: SS
authors.lastname: Meltzer
authors.forename: P L
authors.initials: PL
authors.lastname: Wisnewski
authors.forename: B
authors.initials: B
authors.lastname: White
authors.forename: E R
authors.initials: ER
authors.lastname: Bleecker
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D007249:Inflammation / Q000209:etiology*; D008297:Male; D015203:Reproducibility of Results; D014797:Vital Capacity
chemical_list: D000485:Allergens