[Establishment of trans-inactivation system for haloalkane dehalogenase gene in Arabidopsis thaliana].
With haloalkane dehalogenase gene (dhlA) of Xanthobacter autothophicus as a negative selection marker, a novel trans-inactivation system for transgenes in plants was developed. After the Arabidopsis plants were transformed with dhlA, one plant was shown dhlA post-transcriptional silencing by nuclear run-off transcriptional analysis. The Arabidopsis plants containing silencing locus were crossed to those efficiently expressed dhlA, referred as a homologous transgenic locus, and 96% of F1 plants became the homologous dhlA inactivated in trans. When the F1 plants were selfed, and the silencing locus and the homologous locus were separated in some progenies, 42 of the 200 progenies exhibited DhlA activity, and 158 no DhlA activity, and the proportion of the DhlA-expressing plants to the dhlA-silencing ones was 3.76 to 1, which suggested that the silencing locus inactivated in trans the homologous transgenic locus as a Mendelian dominant factor.
Hao
L
L
Biological Department, Shenyang Teacher's College, Shenyang 110034.
Cao
J
J
Feng
S
S
D017360:Arabidopsis / Q000235:genetics*; D020868:Gene Silencing; D006867:Hydrolases / Q000235:genetics*; D030821:Plants, Genetically Modified
D006867:Hydrolases; C046137:haloalkane dehalogenase