title: Identification of a new biotype of Actinomyces hyovaginalis in tissues of pigs during diagnostic bacteriological examination.
abstract: Bacterial strains isolated from a large variety of necropsy samples of pigs and previously described as a phenotypical homogeneous group were shown to belong to the species Actinomyces hyovaginalis. This was unexpected because their colonial characteristics, as well as their origins, were very different from those originally reported for the vaginal strains on which the species description of A. hyovaginalis was based. Colonial morphology, as well as fermentation of cellobiose, reactions in hippurate and nitrate and production of beta-glucuronidase, allowed separation of the strains studied here from the vaginal strains. Analysis of tRNA intergenic length polymorphisms (tDNA-PCR), 16S rRNA-gene sequencing and DNA-DNA hybridizations were carried out and led to the proposal of a separate biotype within the species A. hyovaginalis. Since, the strains were isolated from different body sites, this biotype has been designated as the 'general' biotype of A. hyovaginalis, while the strains on which the original species description was based are designated as the 'vaginal' biotype.
authors.affiliation: Laboratory of Microbiology, Faculty of Sciences, University of Ghent, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium. virginie.storm@rug.ac.be
authors.forename: V
authors.initials: V
authors.lastname: Storms
authors.forename: J
authors.initials: J
authors.lastname: Hommez
authors.forename: L A
authors.initials: LA
authors.lastname: Devriese
authors.forename: M
authors.initials: M
authors.lastname: Vaneechoutte
authors.forename: T
authors.initials: T
authors.lastname: De Baere
authors.forename: M
authors.initials: M
authors.lastname: Baele
authors.forename: R
authors.initials: R
authors.lastname: Coopman
authors.forename: G
authors.initials: G
authors.lastname: Verschraegen
authors.forename: M
authors.initials: M
authors.lastname: Gillis
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000190:Actinomyces / Q000145:classification* / Q000235:genetics; D000196:Actinomycosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods / Q000662:veterinary*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D012343:RNA, Transfer / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology; D014623:Vaginal Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer