title: Microsurgical repair after crush-avulsion injury of the femoral vein in rats: prevention of microvascular thrombosis with recombinant human tissue-type plasminogen activator (rt-PA).
abstract: Vein thrombosis is often encountered in microsurgery, especially in the case of crush-avulsion injuries. The aim of this study was to investigate the effect of systemic administration of recombinant tissue-type plasminogen activator (rt-PA) on the patency of the femoral vein of the rat, which had previously sustained a crush-avulsion injury. The study consisted of 3 groups of male Wistar rats, 20 animals each. A standardized crush-avulsion injury model was used. After microvascular repair of the femoral vein, the animals received either normal saline (group A), heparin 100 U/kg body weight (group B), or rt-PA 3.5 mg/kg body weight (group C) systemically. Patency tests were performed at 20 minutes, 48 hours, and 1 week after blood flow reestablishment. According to our results, the patency rate of the rt-PA group was significantly higher than in both the control and heparin groups.
authors.affiliation: Microsurgery and Burns Center, Department of Plastic Surgery, General State Hospital of Athens G. Gennimatas, 6 Iktinou Street, 15126 Maroussi, Athens, Greece. elykou@otenet.gr
authors.forename: E G
authors.initials: EG
authors.lastname: Lykoudis
authors.forename: G B
authors.initials: GB
authors.lastname: Contodimos
authors.forename: D A
authors.initials: DA
authors.lastname: Tsoutsos
authors.forename: K B
authors.initials: KB
authors.lastname: Frangia
authors.forename: A E
authors.initials: AE
authors.lastname: Papalois
authors.forename: C N
authors.initials: CN
authors.lastname: Stamatopoulos
authors.forename: J D
authors.initials: JD
authors.lastname: Ioannovich
mesh_terms: D000818:Animals; D005268:Femoral Vein / Q000293:injuries / Q000601:surgery*; D008297:Male; D008866:Microsurgery; D010960:Plasminogen Activators / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D010959:Tissue Plasminogen Activator / Q000627:therapeutic use*; D014654:Vascular Patency; D020246:Venous Thrombosis / Q000517:prevention & control*
chemical_list: D010960:Plasminogen Activators; D010959:Tissue Plasminogen Activator