title: Involvement of gibberellins in expression of a cysteine proteinase (SH-EP) in cotyledons of Vigna mungo seedlings.
abstract: The expression of a papain-type proteinase, designated SH-EP, in cotyledons of Vigna mungo seedlings has been shown to require some factors in the embryonic axes. Gibberellin A1 (GA(1)) and GA(20) were identified by GC-MS in embryonic axes of V. mungo seedlings. The level of accumulation of SH-EP in cotyledons of V. mungo seedlings was greatly reduced by treatment of the seeds with uniconazole-P, an inhibitor for GA biosynthesis. The reduced level of accumulation of SH-EP in cotyledons by uniconazole-P was recovered by exogenous application of GA(1) and GA(20) to the seedlings.
authors.affiliation: Department of Biological Sciences, Tokyo Metropolitan University, Minami-ohsawa, Hachioji, Tokyo, 192-0397 Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taneyama
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: H
authors.initials: H
authors.lastname: Yamane
authors.forename: T
authors.initials: T
authors.lastname: Minamikawa
mesh_terms: D018548:Cotyledon / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D018525:Germination / Q000502:physiology; D005875:Gibberellins / Q000378:metabolism* / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D005875:Gibberellins; D014230:Triazoles; C422660:gibberellin A1; C007842:gibberellic acid; D003546:Cysteine Endopeptidases; C095010:processing enzyme 1, Vigna mungo; C092705:uniconazole