Anti-3AB antibodies in the Chinese yellow cattle infected by the O/Taiwan/99 foot-and-mouth disease virus.
The O/Taiwan/99 foot-and-mouth disease virus (FMDV), a South Asian topotype of serotype O, was introduced into Taiwan in 1999. The Chinese yellow cattle infected by the virus did not develop clinical lesions under experimental and field conditions. A blocking enzyme-linked immunosorbent assay (ELISA) kit with the 3AB antigen, a polypeptide of FMDV non-structural (NS) proteins, was used to evaluate the development and duration of anti-3AB antibodies, proving active viral replication, in the Chinese yellow cattle. The specificity of the assay was 99%, as was established with negative sera from regularly vaccinated and from naïve cattle. The sensitivity tested with sera from naturally infected animals was approximately 64% and it was lower than that obtained by serum neutralization (SN) test. Under experimental infection, the Chinese yellow cattle developed lower anti-3AB antibodies than that developed in other species. Duration of anti-3AB antibodies was traced in two herds of naturally infected animals, indicating that anti-3AB antibodies persisted for approximately 6 months after outbreaks. On the basis of this study, we propose that the Chinese yellow cattle may have natural resistance, which limits viral replication and reduces the development of anti-3AB antibodies.
Huang
Chin-Cheng
CC
Department of Hog Cholera, National Institute for Animal Health, Council of Agriculture, 376 Chung-Cheng Road, Tamsui, Taipei 251, Taiwan, ROC. hc@mail.tahri.gov.tw
Lee
Fan
F
Tu
Wen-Jeng
WJ
Lee
Shu-Hwae
SH
Huang
Ten-Shiang
TS
Lin
Yeou-Liang
YL
Jong
Ming-Hwa
MH
Lin
Shih-Yuh
SY
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis / Q000276:immunology* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D015511:Goat Diseases / Q000276:immunology / Q000821:virology; D006041:Goats; D007113:Immunity, Innate; D009500:Neutralization Tests / Q000662:veterinary; D012680:Sensitivity and Specificity; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014779:Virus Replication
C109754:3ABC protein, virus; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins