title: Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene.
abstract: HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.
authors.affiliation: Department of Tumor Virus and HIV, Institute of Virology, CAPM, Beijing, China.
authors.forename: Guomin
authors.initials: G
authors.lastname: Chen
authors.forename: Shuhui
authors.initials: S
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Xiong
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Ye
authors.forename: Wenping
authors.initials: W
authors.lastname: Dong
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Qimin
authors.initials: Q
authors.lastname: Chen
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Geng
authors.forename: Charles
authors.initials: C
authors.lastname: Wood
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D016915:AIDS Vaccines; D000818:Animals; D002417:Cattle; D002460:Cell Line; D015750:Genes, gag / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D016915:AIDS Vaccines; D011993:Recombinant Fusion Proteins