title: Rapid increase of NO release in plant cell cultures induced by cytokinin.
abstract: 4,5-Diaminofluorescein, a fluorescence indicator for NO, was applied to detect the release of NO from plant cells. NO production was increased within 3 min when plant cell cultures (Arabidopsis, parsley, and tobacco) were treated by cytokinin and was dose-dependent and signal-specific in that other plant hormones and inactive cytokinin analog were not effective in stimulating of NO release. The response was quenched by addition of 2-(aminoethyl)-2-thiopseudourea, an inhibitor of the animal NO synthase, and by addition of an NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-1-oxy-3-oxide. These results imply that NO may act in cytokinin signal transduction.
authors.affiliation: Institut f. Zierpflanzenbau, Baumschule u. Pflanzenzüchtung, Abt. Spez. Ertragsphysiologie, Universität Hannover, Herrenhäuser Str. 2, D-30419, Hannover, Germany.
authors.forename: N N
authors.initials: NN
authors.lastname: Tun
authors.forename: A
authors.initials: A
authors.lastname: Holk
authors.forename: G F
authors.initials: GF
authors.lastname: Scherer
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001593:Benzyl Compounds; D003583:Cytokinins / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D007701:Kinetin; D019684:Magnoliopsida / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D028528:Petroselinum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011687:Purines; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015026:Zeatin / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D003583:Cytokinins; D016166:Free Radical Scavengers; D011687:Purines; D009569:Nitric Oxide; D015026:Zeatin; D019001:Nitric Oxide Synthase; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin