title: Mediator function of the human Rad51B-Rad51C complex in Rad51/RPA-catalyzed DNA strand exchange.
abstract: Five Rad51-like proteins, referred to as Rad51 paralogs, have been described in vertebrates. We show that two of them, Rad51B and Rad51C, are associated in a stable complex. Rad51B-Rad51C complex has ssDNA binding and ssDNA-stimulated ATPase activities. We also examined the functional interaction of Rad51B-Rad51C with Rad51 and RPA. Even though RPA enhances Rad51-catalyzed DNA joint formation via removal of secondary structure in the ssDNA substrate, it can also compete with Rad51 for binding to the substrate, leading to suppressed reaction efficiency. The competition by RPA for substrate binding can be partially alleviated by Rad51B-Rad51C. This recombination mediator function of Rad51B-Rad51C is likely required for the assembly of the Rad51-ssDNA nucleoprotein filament in vivo.
authors.affiliation: Department of Molecular Medicine/Institute of Biotechnology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245-3207, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sigurdsson
authors.forename: S
authors.initials: S
authors.lastname: Van Komen
authors.forename: W
authors.initials: W
authors.lastname: Bussen
authors.forename: D
authors.initials: D
authors.lastname: Schild
authors.forename: J S
authors.initials: JS
authors.lastname: Albala
authors.forename: P
authors.initials: P
authors.lastname: Sung
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D011995:Recombination, Genetic / Q000235:genetics*; D051716:Replication Protein A; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C413002:RAD51B protein, human; C497167:RPA1 protein, human; D051716:Replication Protein A; D000255:Adenosine Triphosphate; D004247:DNA; D000251:Adenosine Triphosphatases