Prenecrotic contracture damage in cardiomyocytes: photochemical fluorochrome staining and fluorescent microscopy of the myocardium.
Prenecrotic contracture changes in cardiomyocytes during ischemic and metabolic alteration of the myocardium are detected at the photooptic level by photochemical fluorochrome staining and examination in fluorescent light. Comparison of the fluorescent and polarization microscopic pictures of damaged cardiomyocytes showed relationships between optically active and isotropic constituents of the cell myofibrillar system. Contracture changes are determined by local mass redistribution in contractile compartments and transformations of the myofibril components, caused by protein structure coagulation in cardiomyocyte sarcomers.
Nepomnyashchikh
L M
LM
Department of Pathology and Pathomorphology, Institute of Regional Pathology and Pathomorphology, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk.
Zimmermann
V G
VG
D000208:Acute Disease; D000818:Animals; D016880:Anisotropy; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006331:Heart Diseases / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D008859:Microscopy, Polarization; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar
D005456:Fluorescent Dyes