Dnmt3b, de novo DNA methyltransferase, interacts with SUMO-1 and Ubc9 through its N-terminal region and is subject to modification by SUMO-1.
Dnmt3b, a DNA methyltransferase, is essential for mammalian development potentially through its transcription repression activity. To comprehend the underlying regulatory mechanism of Dnmt3b, we isolated small ubiquitin-like modifier 1 (SUMO-1) and Ubc9 as Dnmt3b-interacting proteins using yeast two-hybrid screens. Deletion analysis and colocalization experiment demonstrated that Dnmt3b interacts with SUMO-1 and Ubc9 at its N-terminal region. We also confirmed the modification of Dnmt3b by SUMO-1 in vivo. These results suggest that sumoylation may constitute a regulation mechanism of Dnmt3b in vivo.
Kang
E S
ES
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejun, 305-701, South Korea.
Park
C W
CW
Chung
J H
JH
D017398:Alternative Splicing; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D014162:Transfection; D020798:Two-Hybrid System Techniques; D044763:Ubiquitin-Conjugating Enzymes; D000097584:DNA Methyltransferase 3B; D000098843:Ubiquitin-Conjugating Enzyme UBC9
D004248:DNA (Cytosine-5-)-Methyltransferases; D008025:Ligases; D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein; D044763:Ubiquitin-Conjugating Enzymes; D000097584:DNA Methyltransferase 3B; D000098843:Ubiquitin-Conjugating Enzyme UBC9