An in vitro system for testing leucocyte and leukaemic cell line adhesion to synthetic fibres.
Leucocyte adhesion is an important phenomenon in antimicrobial defence, inflammation and immunological mechanisms and has been shown to be dependent upon specialized adhesion molecules. To prevent side-effects related to blood transfusion (e.g. anti-human leucocyte antigen immunization and transmission of infectious agents) leucocyte reduction of blood products is now systematically performed in various countries. The most common system used for leucoreduction is blood filtration. For further understanding of the mechanisms responsible for the interaction between leucocytes and the fibres present in filters we used a flow chamber to study the adhesion of leucocytes and leukaemic cell lines to different types of fibre. Adhesion was quantified using video-microscopy and computer image analysis. Our results demonstrate that adhesion to filter fibres was dependent on the expression of beta2-integrins CD11--CD18 and was inhibited by anti-CD18. The amount of fibres present, their spatial arrangement and the physicochemical characteristics of the fibres were important factors in leucocyte adhesion. Leucocyte adhesion was the highest to polyethylene terephthalate (PET) and polyimide fibres. Lymphocytes or lymphocytic cell lines were poorly adherent to PET fibres. The retaining capacity of leucocyte filters can be improved by taking into account the different parameters for the design of new filters
Barbe
L L
LL
Laboratoire de Biologie Vasculaire et Cellulaire, Paris, and Institut National de la Transfusion Sanguine (INTS), and Institut National de la Recherche Médicale (INSERM) U76, Paris, France.
Boval
B M
BM
Wautier
M P
MP
Wautier
J L
JL
D000475:Alkenes; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000502:physiology; D002482:Cellulose; D005434:Flow Cytometry; D006098:Granulocytes / Q000737:chemistry / Q000502:physiology; D018922:HL-60 Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D016167:Integrin alphaXbeta2 / Q000502:physiology; D019041:L-Selectin / Q000502:physiology; D007937:Leukapheresis / Q000295:instrumentation* / Q000379:methods; D007938:Leukemia / Q000276:immunology*; D007962:Leukocytes / Q000737:chemistry / Q000502:physiology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008214:Lymphocytes / Q000737:chemistry / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000502:physiology; D008851:Micropore Filters; D018715:Microscopy, Video; D009000:Monocytes / Q000737:chemistry / Q000502:physiology; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011126:Polypropylenes; D011142:Polyvinyl Alcohol; D014407:Tumor Cells, Cultured / Q000276:immunology
D000475:Alkenes; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011126:Polypropylenes; D019041:L-Selectin; D011142:Polyvinyl Alcohol; D002482:Cellulose