title: Two cDNAs coding for histamine-gated ion channels in D. melanogaster.
abstract: Histamine, a neurotransmitter and neuroregulatory compound in diverse species, serves as the neurotransmitter of photoreceptors in insects and other arthropods by directly activating a chloride channel. By systematic expression screening of novel putative ligand-gated anion channels, we identified two cDNAs (DM-HisCl-alpha 1 and-alpha 2) coding for putative histamine-gated chloride channels by functional expression in Xenopus laevis oocytes. DM-HisCl-alpha 1 mRNA localizes in the lamina region of the Drosophila eye, supporting the idea that DM-HisCl-alpha 1 may be a neurotransmitter receptor for histamine in the visual system.
authors.affiliation: Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany. guenter.gisselmann@ruhr-uni-bochum.de
authors.forename: Günter
authors.initials: G
authors.lastname: Gisselmann
authors.forename: Hermann
authors.initials: H
authors.lastname: Pusch
authors.forename: Bernd T
authors.initials: BT
authors.lastname: Hovemann
authors.forename: Hanns
authors.initials: H
authors.lastname: Hatt
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D006632:Histamine / Q000378:metabolism* / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D017403:In Situ Hybridization; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014982:Xenopus laevis
chemical_list: D018118:Chloride Channels; D018076:DNA, Complementary; D029721:Drosophila Proteins; C446765:HisCl1 protein, Drosophila; D006635:Histamine H2 Antagonists; C446763:ort protein, Drosophila; D002927:Cimetidine; D006632:Histamine