Formation of protoberberine-type alkaloids by the tubers of somatic embryo-derived plants of Corydalis yanhusuo.
The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA(3)] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog's (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA(3) or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.
Lee
Y L
YL
FooYin Institute of Technology, Department of Biotechnology, Taliou, Kaohsiung County, Taiwan.
Sagare
A P
AP
Lee
C Y
CY
Feng
H T
HT
Ko
Y C
YC
Shaw
J F
JF
Tsay
H S
HS
D000040:Abscisic Acid / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D046508:Culture Techniques; D004365:Drugs, Chinese Herbal; D005875:Gibberellins / Q000494:pharmacology; D028101:Papaveraceae; D018547:Plant Stems / Q000737:chemistry; D011092:Polyethylene Glycols / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D005875:Gibberellins; C452799:corydaline; C009090:protoberberine; D011092:Polyethylene Glycols; C014215:tetrahydropalmatine; D000040:Abscisic Acid; C007842:gibberellic acid