Side chain effect on ion channel characters of Aib rich peptides.
As models of ion channel proteins and naturally occurring pore-forming peptides, we designed a series of Aib rich peptides [Ac-(Aib-Xxx-Aib-Ala)(5)-NH(2) (Xxx = Lys, Glu, Ser, and Gly: BXBA-20)] to investigate the effects of the side chains of the amino acid residues Lys, Glu, Ser, and Gly on the conformation and electrophysiological properties of ion channels. The conformation of peptides and their affinity for phospholipid membranes were evaluated by CD spectroscopy. Patch-clamp experiments revealed that all BXBA-20 peptides form ion channels in DPhPC bilayers exhibiting clearly resolved transitions between the open and closed states. The channel forming frequency was in the order BKBA-20>BEBA-20>BSBA-20>BGBA-20. In the case of BKBA-20 and BEBA-20, the self-assembled conductive oligomers expressed homogeneous and voltage-independent single channel conductances. In contrast, heterogeneous conductance was observed in BSBA-20 and BGBA-20 ion channels under similar experimental conditions. From these results, we conclude that peptides with a high degree of helical conformation, high amphipathicity, high affinity for lipid membranes, and self-associating characters in vesicles are most suitable for inducing ion channels with a high frequency of occurrence. Moreover, BEBA-20, BSBA-20, and BGBA-20 channels were cation-selective, whereas the BKBA-20 channel was non-selective.
Hara
T
T
Department of Chemistry, Faculty of Science and Engineering, Saga University, Saga 840-8502, Japan.
Kodama
H
H
Higashimoto
Y
Y
Yamaguchi
H
H
Jelokhani-Niaraki
M
M
Ehara
T
T
Kondo
M
M
D000621:Aminoisobutyric Acids / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D015195:Drug Design; D004594:Electrophysiology; D007473:Ion Channels / Q000737:chemistry / Q000502:physiology*; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D015202:Protein Engineering; D017433:Protein Structure, Secondary
D000621:Aminoisobutyric Acids; D007473:Ion Channels; D008051:Lipid Bilayers; D010455:Peptides; C100049:2-aminoisobutyric acid