The effect of beta-carotene on the expression of interleukin-6 and heme oxygenase-1 in UV-irradiated human skin fibroblasts in vitro.
beta-Carotene is discussed as an anti-oxidant micronutrient and singlet oxygen quencher in human skin, protecting against UV light-induced damage. However, we recently demonstrated that beta-carotene has a pro-oxidant potential in cultured human skin fibroblasts because it enhances the UVA induction of heme oxygenase-1 (HO-1). Herein, we further show that beta-carotene also strongly promotes the UVA induction of pro-inflammatory interleukin-6 (IL-6) in skin fibroblasts in vitro. Singlet oxygen quencher sodium azide abrogated up-regulation of IL-6, and likewise also of HO-1. In UVB-irradiated cells, beta-carotene did not modulate levels of IL-6 and HO-1. The observed effects might be relevant for UV-induced inflammatory processes.
Obermüller-Jevic
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UC
Department of Biological Chemistry and Nutrition, University of Hohenheim, Fruwirthstrasse 12, 70593, Stuttgart, Germany. biesal@uni-hohen.heim.de
Schlegel
B
B
Flaccus
A
A
Biesalski
H K
HK
D002675:Child, Preschool; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D008565:Membrane Proteins; D012867:Skin / Q000166:cytology* / Q000528:radiation effects; D019810:Sodium Azide / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*; D019207:beta Carotene / Q000494:pharmacology*
D015850:Interleukin-6; D008565:Membrane Proteins; D019207:beta Carotene; D019810:Sodium Azide; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1