DNA repair of hydrogen peroxide-induced damage in human lymphocytes in the presence of four antimutagens. A study with alkaline single cell gel electrophoresis (comet assay).
We assessed four antimutagenic compounds' influences on DNA repair in human lymphocytes exposed in vitro to hydrogen peroxide (20 microM, 5 min, at 4 degrees C). DNA damage and repair were estimated by means of alkaline single cell gel electrophoresis (comet assay). It was noticed that the enhancement of DNA repair was relatively strongest when fluphenazine was present in the cell culture medium. In the cases of anthocyanins and alkylresorcinols, the effects were almost 6-9 times weaker than that of FPh. The effect of todralazine on DNA repair was relatively weakest. Further study should be done on fluphenazine as a potential DNA repair-enhancing compound.
Gasiorowski
K
K
Wrocław Medical University, Department of Basic Medical Sciences, Kochanowskiego 14, 51-601 Wrocław, Poland. kaz@basmed.am.wroc.pl
Brokos
B
B
D000328:Adult; D000872:Anthocyanins / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000494:pharmacology*; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects*; D005476:Fluphenazine / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012118:Resorcinols / Q000494:pharmacology; D014032:Todralazine / Q000494:pharmacology
D000872:Anthocyanins; D016587:Antimutagenic Agents; D012118:Resorcinols; D006861:Hydrogen Peroxide; D005476:Fluphenazine; D014032:Todralazine