[Effect of EGTA and La2+ on induction of sesquiterpene cyclase gene expression in leaves of Capsicum annuum by several abiotic elicitors].
To destroy Ca2+ signal system, the detached leaves of Capsicum annuum were pretreated with EGTA, a commonly used Ca2+ chelator, or La3+, an ion which competitively inhibits Ca2+ movement through plasma membrane channels. The induction of sesquiterpene cyclase gene expression in the pretreated pepper leaves under the treatments of CuCl2, HgCl2 and UV was conducted in this paper. The result showed that no hindrance of sesquiterpene cyclase enzyme activity was detected in the EGTA or LaCl3 pretreated leaves, compared to the controls treated with CuCl2, HgCl2, and UV without the pretreatment, while the pretreatment with EGTA enhanced the inductivity of the above exogenous abiotic elicitors on the enzyme activity. The sesquiterpene cyclase activity was also detected in leaves treated with EGTA only, and the result of Northern Blot analysis indicated that the enhancement of EGTA on the enzyme activity was due to the induction of EGTA on the gene transcription. It is suggested that there might exist other signal pipelines, in addition to the Ca2+ signal system in sesquiterpene cyclase gene expression in pepper leaves, and the mechanism of induction of abiotic elicitors on sesquiterpene cyclase in C. annuum differed from that of biotic elicitors.
He
S
S
College of Crop Science, Fujian Agricultural University, Fuzhou 350002. shlhe@pub5.fz.fj.cn
Lin
W
W
Chen
R
R
D020013:Calcium Signaling; D002212:Capsicum / Q000378:metabolism*; D019755:Carbon-Carbon Lyases / Q000235:genetics*; D004533:Egtazic Acid / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007811:Lanthanum / Q000494:pharmacology*
C028521:lanthanum chloride; D004533:Egtazic Acid; D007811:Lanthanum; D019755:Carbon-Carbon Lyases; C051130:trichodiene synthetase