title: Measurement of Ca(2+) currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique.
abstract: The recording of currents passing through calcium channels in intact skeletal muscle fibers presents several difficulties. However, use of the three-microelectrode voltage-clamp technique at the end of the fiber provided us with a good approximation of current values in such fibers. Using this technique, we were able to measure the calcium-channel current in slow skeletal muscle fibers of the frog (Rana pipiens) and to quantify the effects of denervation on this current.
authors.affiliation: Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Apartado Postal No. 11, 28000-Colima, Colima, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Vásquez
authors.forename: M
authors.initials: M
authors.lastname: Huerta
authors.forename: X
authors.initials: X
authors.lastname: Trujillo
authors.forename: J
authors.initials: J
authors.lastname: Luis Marín
authors.forename: F
authors.initials: F
authors.lastname: Andrade
authors.forename: B
authors.initials: B
authors.lastname: Trujillo-Hernández
mesh_terms: D000818:Animals; D001464:Barium; D015220:Calcium Channels / Q000378:metabolism*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008839:Microelectrodes; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D011894:Rana pipiens; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D015220:Calcium Channels; D001464:Barium