title: ENA-78 is an important angiogenic factor in idiopathic pulmonary fibrosis.
abstract: Idiopathic pulmonary fibrosis (IPF) is a chronic and often fatal disorder. Fibroplasia and deposition of extracellular matrix are dependent, in part, on angiogenesis and vascular remodeling. We obtained open lung biopsies from patients undergoing thoracic surgery for reasons other than interstitial lung disease (control) (n = 78) and from patients with IPF (n = 91). We found that levels of epithelial neutrophil-activating peptide 78 (ENA-78) were greater from tissue specimens of IPF patients, as compared with control subjects. When ENA-78 was depleted from IPF tissue specimens, tissue-derived angiogenic activity was markedly reduced. Immunolocalization of ENA-78 demonstrated that hyperplastic Type II pneumocytes and macrophages were the predominant cellular sources of ENA-78. These findings support the notion that ENA-78 may be an important additional factor that regulates angiogenic activity in IPF.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095-1922, USA. mpkeane@mednet.ucla.edu
authors.forename: M P
authors.initials: MP
authors.lastname: Keane
authors.forename: J A
authors.initials: JA
authors.lastname: Belperio
authors.forename: M D
authors.initials: MD
authors.lastname: Burdick
authors.forename: J P
authors.initials: JP
authors.lastname: Lynch
authors.forename: M C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: R M
authors.initials: RM
authors.lastname: Strieter
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D003315:Cornea / Q000098:blood supply; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000378:metabolism / Q000502:physiology*; D008168:Lung / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D011658:Pulmonary Fibrosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: D043925:Angiogenesis Inducing Agents; C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D016209:Interleukin-8