Effects of erysimin G on renal tubular function and 70-pS K+ channel activity of thick ascending limb.
OBJECTIVE
To study the effect of erysimin G (CH35H52O13) on the thick ascending limb (TAL) 70-pS K+ channel of rat kidney and its effect on diuresis.


METHODS
The patch-clamp cell-attached recording technique was used to record the single potassium channel current, and the urine volume (UV) was measured by urethral intubation to determine the diuretic effect.


RESULTS
Erysimin G can increase the urine volume and decrease the 70-pS potassium channel activity of TAL.


CONCLUSIONS
Erysimin G has a diuretic effect and its inhibition on the activity of apical 70-pS potassium channel may be the mechanism of its diuretic effect.
Shan
H L
HL
Department of Pharmacology, Harbin Medical University, Harbin 150086, China.
Zhang
X D
XD
Gu
R M
RM
Luo
D L
DL
Yang
B F
BF
D000818:Animals; D002301:Cardiac Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D004232:Diuretics / Q000302:isolation & purification / Q000494:pharmacology*; D032601:Erysimum / Q000737:chemistry; D005260:Female; D007686:Kidney Tubules, Distal / Q000502:physiology*; D008297:Male; D018408:Patch-Clamp Techniques; D010946:Plants, Medicinal / Q000737:chemistry; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D014556:Urine
D002301:Cardiac Glycosides; D004232:Diuretics; D015221:Potassium Channels; C478419:erysimin G