Antithrombotic effect of geniposide and genipin in the mouse thrombosis model.
Geniposide is one of the constituents of Gardenia fruit (Gardenia jasminoides Ellis, Rubiaceae), which has been used in traditional medicine. Although its anti-inflammatory and antithrombotic effects have been reported, the way it acts is still unclear. We have investigated the effects of geniposide and its metabolite genipin on thrombogenesis and platelet aggregation. In an in vivo model, geniposide and genipin significantly (P < 0.05) prolonged the time required for thrombotic occlusion induced by photochemical reaction in the mouse femoral artery. In an in vitro study, both geniposide and genipin inhibited collagen-induced, but did not inhibit arachidonate-induced, mouse platelet aggregation. However aspirin, a cyclooxygenase inhibitor, inhibited arachidonate-induced platelet aggregation but only partially inhibited the collagen-induced one. We also showed, by measuring PLA(2)-catalyzed arachidonic acid release, that geniposide inhibited phospholipase A(2) (PLA(2)) activity. We conclude that geniposide showed an antithrombotic effect in vivo due to the suppression of platelet aggregation. PLA(2) inhibition by geniposide is one possible anti-platelet mechanism.
Suzuki
Y
Y
Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Japan. yapplel@hama-med.ac.jp
Kondo
K
K
Ikeda
Y
Y
Umemura
K
K
D000818:Animals; D004195:Disease Models, Animal; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D005638:Fruit / Q000737:chemistry; D057889:Iridoid Glycosides; D039823:Iridoids; D008297:Male; D051379:Mice; D010741:Phospholipases A / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D010974:Platelet Aggregation / Q000187:drug effects; D011714:Pyrans / Q000737:chemistry / Q000627:therapeutic use*; D019666:Rubiaceae; D013927:Thrombosis / Q000139:chemically induced / Q000188:drug therapy*
D005343:Fibrinolytic Agents; D057889:Iridoid Glycosides; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; C007835:geniposide; C007834:genipin; D010741:Phospholipases A