Action sites of adrenomedullin in the rat brain: functional mapping by Fos expression.
The effects of intracerebroventricular (icv) administration of adrenomedullin (AM) and proadrenomedullin NH2-terminal 20 peptide (PAMP) on the expression of Fos in the central nervous system (CNS) were examined in conscious rats, using immunohistochemistry. Fos-like immunoreactivity (LI) was detected in various brain areas of the rats, including the supraoptic nucleus, the paraventricular nucleus, the locus coeruleus, the area postrema and the nucleus of the tractus solitarius 90 min after icv administration of AM. Few cells with Fos-LI were found in the CNS 90 min after icv administration of saline. Fos-LI was also detected in the various hypothalamic areas after icv administration of PAMP. These results suggest that centrally administered AM and PAMP may cause physiological responses through the activation of a neural network in the hypothalamus and the brainstem.
Ueta
Y
Y
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, 807-8555, Yahatanishi-ku, Kitakyushu, Japan. yoichi@med.uoeh-u.ac.jp
Hara
Y
Y
Kitamura
K
K
Kangawa
K
K
Eto
T
T
Hattori
Y
Y
Yamashita
H
H
D053607:Adrenomedullin; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats
D010446:Peptide Fragments; D010455:Peptides; D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos; D053607:Adrenomedullin