DNA replication. Genomic views of genome duplication.
DNA replication is initiated at numerous origins of replication (oris) within the chromosomes. In a pair of ambitious studies, two groups have used different techniques to pinpoint the locations of all of the oris throughout the yeast genome at different times during S phase (Raghuraman et al., Wyrick et al.). Stillman, in his Perspective, compares and contrasts the different methods and their findings, and speculates on the value of combining these techniques to look at oris in the human genome.
Stillman
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Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA. stillman@cshl.org
D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000378:metabolism; D015825:Chromosomes, Fungal; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000378:metabolism; D016681:Genome, Fungal; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D051738:Origin Recognition Complex; D018741:Replication Origin; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D051738:Origin Recognition Complex; D029701:Saccharomyces cerevisiae Proteins