title: Description and validation of a staggered parallel high performance liquid chromatography system for good laboratory practice level quantitative analysis by liquid chromatography/tandem mass spectrometry.
abstract: The Aria LX4 staggered parallel high performance liquid chromatography (HPLC) system is evaluated for application to good laboratory practice (GLP) level quantitative analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS). This system consists of four fully independent binary HPLC pumps, a modified autosampler, and a series of switching and selector valves all controlled by a single computer program. The system improves sample throughput without sacrificing chromatographic separation or data quality. Validation results for four different compounds, each analyzed on a separate channel of the Aria system, show precision and accuracy equivalent to that required of a single-channel system. The results show that sample throughput can be increased nearly four-fold without requiring significant changes in current analytical procedures. The flexibility and ease of use of the Aria system suggest that it should be possible to quickly implement it in any analytical LC/MS/MS environment.
authors.affiliation: Department of Drug Metabolism, Merck and Co., Inc., West Point, PA 19486, USA. richard_king@merck.com
authors.forename: Richard C
authors.initials: RC
authors.lastname: King
authors.forename: Cynthia
authors.initials: C
authors.lastname: Miller-Stein
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Magiera
authors.forename: John
authors.initials: J
authors.lastname: Brann
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions
chemical_list: D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012996:Solutions