Association of the mitochondrial DNA 5178 A/C polymorphism with serum lipid levels in the Japanese population.
As one approach to exploring whether the mitochondrial DNA 5178 adenine/cytosine (mt5178 A/C) polymorphism is associated with atherosclerosis, we genotyped 461 healthy Japanese individuals and studied the relationship of mt5178 A/C genotypes to serum lipid levels. Blood specimens were obtained after at least a 12-h fasting period from the subjects. The mt5178 A/C was genotyped by the polymerase chain reaction/restriction fragment length polymorphism method. The relative frequency of mt5178 A was 41.6% (192/461) and of mt5178 C was 58.4% (269/461). After adjustments for age and body mass index, the high-density lipoprotein cholesterol concentration in males carrying mt5178 A was significantly higher than that in males carrying mt5178 C ( P=0.026). The tryglyceride (TG) concentration in females carrying mt5178 A was significantly lower than that in females carrying mt5178 C ( P=0.012). This difference in the TG level between the two genotypes was more evident in postmenopausal females than in premenopausal females. Mt5178 A seems to have an antiatherogenic effect. This is the first genetic epidemiological report on the association of mt5178 A/C polymorphism with serum lipid levels in the Japanese population.
Kokaze
A
A
Department of Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo, 181-8611, Japan. dawn@kyorin-u.ac.jp
Ishikawa
M
M
Matsunaga
N
N
Yoshida
M
M
Sekine
Y
Y
Teruya
K
K
Takeda
N
N
Sumiya
Y
Y
Uchida
Y
Y
Takashima
Y
Y
D000225:Adenine; D001483:Base Sequence; D002784:Cholesterol / Q000097:blood* / Q000145:classification; D003596:Cytosine; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D014280:Triglycerides / Q000097:blood*
D017931:DNA Primers; D004272:DNA, Mitochondrial; D014280:Triglycerides; D003596:Cytosine; D002784:Cholesterol; D000225:Adenine