[Establishment of an in vitro model for rheumatoid arthritis as test system for therapeutical substances].
In our Tissue Engineering group a 3D in vitro model for rheumatoid Arthritis (in vitro pannus) was established with the aim to develop a standardized drug-screening test to analyze the effects of drugs and different biological substances. The advanced model consists of chondrocyte pellet cultures interacting with rheumatoid arthritis (RA) synovial cell cultures. To establish interactive 3D co-cultures defined rheumatoid arthritis synovial cell populations were centrifuged directly on chondrocyte pellet cultures. Histochemical stainings during time of co-culture revealed obvious invasion by RA synovial cell populations into the chondrocyte matrix. Gene expression analysis showed a downregulation of collagen type II expression in chondrocytes within 2 weeks after co-culture with RA synovial cells. Those interactive co-cultures allow the study of single cell populations as well as the cellular interactions in this system under in vitro conditions. Thus, the established co-culture model may be suitable for routine screening tests, which can be useful in supplementing animal experiments in basic research and drug testing.
Smolian
H
H
TransTissue Technologies GmbH, Berlin, Germany. heike.smolian@transtissue.com
Thiele
S
S
Kolkenbrock
H
H
Zacher
J
J
Aicher
W
W
Schultz
O
O
Burmester
G R
GR
Sittinger
M
M
D000826:Animal Testing Alternatives; D000818:Animals; D001172:Arthritis, Rheumatoid; D006801:Humans; D012106:Research / Q000592:standards*; D012107:Research Design