title: A novel second myostatin gene is present in teleost fish.
abstract: We report on the isolation and characterisation of the complete cDNA sequence encoding a novel bone morphogenetic protein-like protein (sbMSTN-b) in the teleost fish Sparus aurata. The encoded protein is 68% identical to S. aurata MSTN at the amino acid level, and homologues were also found in Umbrina cirrosa and Tetraodon nigroviridis. Phylogenetic analysis suggests that the MSTN-b gene may be present in most, perhaps all, teleost fish species. RT-PCR on different tissues/stages indicates that MSTN-b is expressed almost exclusively in the central nervous system, starting from late larval stages. Quantitative analyses indicate an increase of sbMSTN-b expression in the brain associated with metamorphosis, at the same time as completion of nervous system differentiation.
authors.affiliation: Dipartamento di Biologia, Università di Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Maccatrozzo
authors.forename: L
authors.initials: L
authors.lastname: Bargelloni
authors.forename: B
authors.initials: B
authors.lastname: Cardazzo
authors.forename: G
authors.initials: G
authors.lastname: Rizzo
authors.forename: T
authors.initials: T
authors.lastname: Patarnello
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008969:Molecular Sequence Data; D055435:Myostatin; D009420:Nervous System / Q000196:embryology; D010473:Perciformes; D010802:Phylogeny; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D055435:Myostatin; D020033:Protein Isoforms; D016212:Transforming Growth Factor beta