title: Effects and mechanisms of triacetylshikimic acid on platelet adhesion to neutrophils induced by thrombin and reperfusion after focal cerebral ischemia in rats.
abstract: OBJECTIVE
  To investigate the effect of triacetylshikimic acid (TSA) on the platelet adhesion to neutrophils and P-selectin expression on activated platelet membrane induced by thrombin and reperfusion after focal cerebral ischemia.
  
  
  METHODS
  The platelet adhesion to neutrophils was evaluated by rosette assay, and P-selectin expression on platelet membrane was determined by flow cytometry.
  
  
  RESULTS
  TSA 10 - 1000 micromol/L markedly inhibited thrombin(0.4 kU/L)-induced platelet adhesion to neutrophils. The platelet adhesion to neutrophils induced by a 21-h reperfusion after middle cerebral artery occlusion for 3 h was also inhibited in a dose-dependent manner by TSA 50 - 200 mg/kg given by ig immediately and at 60 min again after the onset of cerebral ischemia. TSA was also shown to decrease the P-selectin expression on platelet surface induced by thrombin in washed platelet and by adenosine diphosphate (ADP) 5 micromol/L in whole blood.
  
  
  CONCLUSIONS
  Reperfusion after cerebral ischemia and thrombin induced platelet adhesion to neutrophils, which could be reduced by TSA probably due to its inhibition of P-selectin expression on activated platelets.
authors.affiliation: Department of Pharmacology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Chong
authors.forename: Q P
authors.initials: QP
authors.lastname: Xu
authors.forename: J N
authors.initials: JN
authors.lastname: Sun
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000097:blood*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology*; D019007:P-Selectin / Q000096:biosynthesis; D010973:Platelet Adhesiveness / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D018696:Neuroprotective Agents; D019007:P-Selectin; C452560:triacetylshikimic acid; D012765:Shikimic Acid; D013917:Thrombin