Mutations in dihydropteroate synthase gene of Pneumocystis carinii in HIV patients with Pneumocystis carinii pneumonia.
The purpose of this study was to determine whether dihydropteroate synthase gene (DHPS) mutations were associated with the failure of sulpha/sulphone drugs used as prophylaxis agents in HIV infected patients. Results suggested that DHPS mutations were significantly associated with failure of anti-Pneumocystis carinii sulphone prophylaxis (P=0.031). An increasing number of mutant P. carinii strains have been isolated from patients no longer having prophylaxis. There was no statistically significant difference in severity or outcome of the pneumonia caused by wild-type or mutant DHPS. Moreover, two of the three patients with mutant P. carinii pneumonia (PCP) were successfully treated with sulpha drugs. We think that P. carinii drug-resistance could be an emerging problem for immunocompromised patients including those with HIV infection.
Visconti
E
E
Department of Infectious Diseases, Università Cattolica del S. Cuore, Largo F. Vito, 1 00168, Rome, Italy.
Ortona
E
E
Mencarini
P
P
Margutti
P
P
Marinaci
S
S
Zolfo
M
M
Siracusano
A
A
Tamburrini
E
E
D000328:Adult; D004094:Dihydropteroate Synthase / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000188:drug therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011010:Pneumocystis / Q000201:enzymology / Q000235:genetics*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology* / Q000517:prevention & control; D013450:Sulfones / Q000627:therapeutic use; D017211:Treatment Failure
D013450:Sulfones; D004094:Dihydropteroate Synthase