title: Antiadenovirus activity of milk proteins: lactoferrin prevents viral infection.
abstract: Different milk proteins were analysed for their inhibitory effect on adenovirus infection in vitro. Proteins investigated were mucin, alpha-lactalbumin, beta-lactoglobulin, bovine lactoferrin, and human lactoferrin. Results obtained demonstrated that mucin, alpha-lactalbumin, and beta-lactoglobulin did not prevent the viral cytopathic effect, whereas lactoferrin was able to inhibit adenovirus replication in a dose-dependent manner. Further experiments were carried out in which lactoferrin was added to the cells during different phases of viral infection. Results obtained showed that lactoferrin was able to prevent viral replication when added both before, or during the viral adsorption step, or when present during the entire replicative cycle of adenovirus, demonstrating that its action takes place on an early phase of viral replication.
authors.affiliation: Institute of Microbiology, University La Sapienza, Rome, Italy.
authors.forename: Daria
authors.initials: D
authors.lastname: Arnold
authors.forename: Assunta Maria
authors.initials: AM
authors.lastname: Di Biase
authors.forename: Magda
authors.initials: M
authors.lastname: Marchetti
authors.forename: Agostina
authors.initials: A
authors.lastname: Pietrantoni
authors.forename: Piera
authors.initials: P
authors.lastname: Valenti
authors.forename: Lucilla
authors.initials: L
authors.lastname: Seganti
authors.forename: Fabiana
authors.initials: F
authors.lastname: Superti
mesh_terms: D000258:Adenovirus Infections, Human / Q000517:prevention & control* / Q000821:virology; D000260:Adenoviruses, Human / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology*; D008894:Milk Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D008894:Milk Proteins; D007781:Lactoferrin