An extensive monoclonal antibody panel for the phenotyping of leukocyte subsets in the common marmoset and the cotton-top tamarin.
New World monkeys are valuable animal models to study human diseases. To determine the phenotype of cells involved in immune responses, we used flow cytometry to screen a large panel of anti-human monoclonal antibodies (mAb) for cross-reactivity with cells of the common marmoset and the cotton-top tamarin. Certain antigens (e.g., CD2, CD8, CD20) are well conserved. However, CD10, CD23, and CD33 showed a clear discrepancy in their reaction patterns in both species, indicating that significant differences on the epitope level occurred during evolution. Epstein-Barr virus-transformed B-cell lines were shown to be a valuable tool for screening B-cell-specific reagents. In some cases, fluorescein isothiocyanate (FITC) and phycoerythrin (PE) modification of mAbs had a negative effect on the binding capacity, which stressed the importance of choosing the right label. Despite the fact that some CD antigens were not detected, adequate numbers of cross-reactive mAbs were identified to perform extensive studies on immunological functions in both the common marmoset and the cotton-top tamarin.
Brok
H P
HP
Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. brok@bprc.nl
Hornby
R J
RJ
Griffiths
G D
GD
Scott
L A
LA
Hart
B A
BA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000276:immunology; D002144:Callithrix / Q000097:blood*; D003429:Cross Reactions; D005260:Female; D005434:Flow Cytometry; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000145:classification / Q000166:cytology / Q000276:immunology*; D008297:Male; D012452:Saguinus / Q000097:blood*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD