title: Chiral separation of beta-methyl-amino acids by ligand exchange using capillary electrophoresis and HPLC.
abstract: This paper deals with the chiral separation of optical isomers of beta-methyl-amino acids by CE and HPLC using the principle of ligand-exchange. Capillary zone electrophoresis was carried out using Cu(II) complexes of L-4-hydroxyproline (L-4-Hypro), N-(2-hydroxypropyl)-L-4-hydroxyproline (HP-L-4-Hypro) and N-(2-hydroxyoctyl)-L-4-hydroxyproline (HO-L-4-Hypro) as chiral selectors, added to the electrolyte. The HPLC separations were performed on a chiral stationary ligand-exchange chromatography phase containing L-4-Hypro chemically bonded to silica gel. With both techniques nearly all compounds investigated are baseline resolved using different background electrolytes and mobile phases, respectively.
authors.affiliation: Institute of Pharmaceutical Chemistry, Karl-Franzens University, Graz, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Grobuschek
authors.forename: M G
authors.initials: MG
authors.lastname: Schmid
authors.forename: C
authors.initials: C
authors.lastname: Tuscher
authors.forename: M
authors.initials: M
authors.lastname: Ivanova
authors.forename: G
authors.initials: G
authors.lastname: Gübitz
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D008024:Ligands; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D008024:Ligands