Antiproliferative and antimitogenic activities in a peptide from puffball mushroom Calvatia caelata.
A peptide with a molecular weight of 8 kDa and an N-terminal sequence closely resembling that of ubiquitin was isolated from fruiting bodies of the mosaic puffball mushroom Calvatia caelata. The peptide was purified using a protocol that involved ion-exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and ion-exchange chromatography on Mono S. The peptide inhibited translation in the cell-free rabbit reticulocyte lysate system and exhibited N-glycosidase activity. It potently inhibited proliferation of spleen cells with an IC(50) of about 100 nM as indicated by the suppression of [methyl-(3)H]thymidine uptake. The viability of breast cancer cells was reduced to half at a ubiquitin concentration of about 100 nM.
Lam
Y W
YW
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Ng
T B
TB
Wang
H X
HX
D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001943:Breast Neoplasms / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006026:Glycoside Hydrolases / Q000378:metabolism; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011817:Rabbits; D012260:Ribonucleases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D025801:Ubiquitin / Q000737:chemistry
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D010455:Peptides; D025801:Ubiquitin; D012260:Ribonucleases; D006026:Glycoside Hydrolases