Functional genomics: the coming of age for Tetrahymena thermophila.
Over the past decade, researchers have manipulated the unique biology of Tetrahymena thermophila to generate a premier experimental organism for functional genomic analysis. A diverse array of DNA transformation methods have spearheaded in vivo strategies for discovering and dissecting universal eukaryotic processes, such as telomere addition and chromatin remodeling. Compartmentalization of this protist's genome into two functionally distinct nuclei - the silent 'germline' micronucleus and the transcriptionally active macronucleus - provides a powerful means for controlling the expression of transgenes. Heterokaryons that silently harbor homozygous recessive mutations (including lethal ones) in the germline have been exploited. The coupling of forward and reverse genetic approaches with genomics-based methods for gene discovery presents a bright future for research in this rising model eukaryote.
Turkewitz
Aaron P
AP
Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 E. 58th Street, Chicago, IL 60637, USA. apturkew@midway.uchicago.edu
Orias
Eduardo
E
Kapler
Geoffrey
G
D000818:Animals; D016375:Antisense Elements (Genetics); D005821:Genetic Techniques; D005822:Genetic Vectors; D018503:Genome, Protozoan; D023281:Genomics; D016808:Tetrahymena thermophila / Q000235:genetics*; D014170:Transformation, Genetic
D016375:Antisense Elements (Genetics)