title: Multiple diffraction data analysis for macromolecular crystals in stereoscopic multibeam imaging.
abstract: A detailed analysis of multiple diffraction data collected by the stereoscopic multibeam imaging technique from a tetragonal lysozyme crystal is reported. Calculations based on the dynamical theory are employed to account for diffraction profiles obtained with Bragg-angle scan in stereoscopic imaging and the conventional azimuthal scan in Renninger arrangement. The formation of a multibeam intensity profile and the relationship and mutual influence between the two scans are investigated. A simple practical method of quantitative estimation of the reflection phases of structure-factor multiplets from the experimental data obtained with two inversion-symmetry-related diffractions is proposed. The procedures for data handling and for distinguishing "partial" diffraction images from "full" diffraction images are also developed considering multibeam diffraction geometry and experimental conditions. These procedures thus provide a practical way of reconstructing diffraction profiles for experimental phase determination for macromolecular crystals.
authors.affiliation: Department of Physics, National Tsing Hua University, Hsinchu, Taiwan 300, ROC.
authors.forename: Chun Hsiung
authors.initials: CH
authors.lastname: Chao
authors.forename: Chi Yuan
authors.initials: CY
authors.lastname: Hung
authors.forename: Yi Shan
authors.initials: YS
authors.lastname: Huang
authors.forename: Chia Hung
authors.initials: CH
authors.lastname: Ching
authors.forename: Yen Ru
authors.initials: YR
authors.lastname: Lee
authors.forename: Yuch Chen
authors.initials: YC
authors.lastname: Jean
authors.forename: Shu Ching
authors.initials: SC
authors.lastname: Lai
authors.forename: Yuri P
authors.initials: YP
authors.lastname: Stetsko
authors.forename: Hanna
authors.initials: H
authors.lastname: Yuan
authors.forename: Shih Lin
authors.initials: SL
authors.lastname: Chang