Puerarin blocks transient outward K+ current and delayed rectifier K+ current in mice hippocampal CA1 neurons.
OBJECTIVE
To study the effects of puerarin (Pue) on IA and IK in mouse hippocampal neurons.


METHODS
The whole cell patch clamp techniques were used.


RESULTS
Pue reduced the amplitude of IA and IK, in a concentration-dependent, but not rate- or use-dependent manner (IC50 were 461 micromol/L and 215 micromol/L, respectively). Pue (0.5 mmol/L) shifted the steady state activation curves of IA and IK to positive and negative potentials (Vh about 20.6 mV and 28.6 mV) respectively, but inactivation curves of IA were not affected by Pue.


CONCLUSIONS
Pue inhibited IA and IK in mouse hippocampal CA1 neurons and its blocking effect on I(K) was much stronger than on IA.
Zhang
G Q
GQ
National Laboratory of Biomembrane and Membrane Biotechnology, Peking University, Beijing 100871, China.
Hao
X M
XM
Zhou
P A
PA
Wu
C H
CH
Dai
D Z
DZ
D000818:Animals; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D015221:Potassium Channels / Q000145:classification / Q000187:drug effects*; D017966:Pyramidal Cells / Q000187:drug effects
D007529:Isoflavones; D018696:Neuroprotective Agents; D026902:Potassium Channel Blockers; D015221:Potassium Channels; C033607:puerarin