Use of FT-NIR transmission spectroscopy for the quantitative analysis of an active ingredient in a translucent pharmaceutical topical gel formulation.
The objective of this study was to demonstrate the use of transmission Fourier transform near-infrared (FT-NIR) spectroscopy for quantitative analysis of an active ingredient in a translucent gel formulation. Gels were prepared using Carbopol 980 with 0%, 1%, 2%, 4%, 6%, and 8% ketoprofen and analyzed with an FT-NIR spectrophotometer operated in the transmission mode. The correlation coefficient of the calibration was 0.9996, and the root mean squared error of calibration was 0.0775%. The percent relative standard deviation for multiple measurements was 0.10%. The results prove that FT-NIR can be a good alternative to other, more time-consuming means of analysis for these types of formulations.
Kemper
M S
MS
Thermo Nicolet Corporation, Madison, WI 53711, USA. kemper@thermonicolet.com
Magnuson
E J
EJ
Lowry
S R
SR
McCarthy
W J
WJ
Aksornkoae
N
N
Watts
D C
DC
Johnson
J R
JR
Shukla
A J
AJ
D000287:Administration, Topical; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis*; D005583:Fourier Analysis; D005782:Gels; D007660:Ketoprofen / Q000008:administration & dosage / Q000032:analysis*; D019265:Spectroscopy, Near-Infrared
D000894:Anti-Inflammatory Agents, Non-Steroidal; D005782:Gels; D007660:Ketoprofen