Streptozocin-induced pancreatic islet destruction in beef cows.
Streptozocin (STZ) induces diabetes mellitus in sheep and pigs. To test the effect of STZ in cattle, cows were given 75-150 mg STZ per kilogram of body weight. Cows receiving 150 mg/kg required euthanasia within 24 hours after infusion because of the severe systemic effects of STZ. Seven cows receiving doses of < or = 100 mg/kg had mild to marked decrease in islet immunoreactivity for insulin and in pancreatic islet density and mild to severe tubulointerstitial nephritis. Two cows receiving 75 and 85 mg/kg STZ regained their ability to produce insulin and return blood glucose to basal levels. One cow given 100 mg/kg STZ developed insulin insufficiency consistent with type I diabetes mellitus. These findings demonstrate the susceptibility of the bovine pancreas to STZ: however, severe systemic complications were encountered. Alternative dosages and methodologies should be considered in future attempts to induce diabetes in cattle using STZ.
Higdon
H L
HL
Reproductive Endocrinology and Infertility, Greenville Hospital System, SC 29605-5601, USA. lhigdon@ghs.org
Parnell
P G
PG
Hill
J E
JE
Spitzer
J C
JC
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005951:Glucose Tolerance Test / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D007328:Insulin / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D013311:Streptozocin
D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin