DNA probes using fluorescence resonance energy transfer (FRET): designs and applications.
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed.
Didenko
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Baylor College of Medicine, Houston, TX, USA. vdidenko@bcm.tmc.edu
D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D004735:Energy Transfer; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D013050:Spectrometry, Fluorescence / Q000379:methods*
D015342:DNA Probes; D004247:DNA