title: Cooling inhibits capsaicin-induced currents in cultured rat dorsal root ganglion neurones.
abstract: Whole-cell and single-channel recordings from rat dorsal root ganglion neurones were used to investigate the temperature dependence of currents through the capsaicin receptor (vanilloid receptor 1, VR1). Reducing the temperature from 31 to 14 degrees C inhibited the current induced by 0.5 microM capsaicin by 80%. The Q(10) (temperature coefficient over a 10 degrees C range) of the whole-cell capsaicin-induced current was 2.3 between 10 and 30 degrees C. Single-channel recordings showed that this inhibition by cooling was due to a marked reduction in the open probability (Q(10)=8.2 between 10 and 30 degrees C). This effect can explain the pain relief and reduction in inflammation caused by strong cooling of the skin.
authors.affiliation: Department of Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independenţei 91-95, 76201 Bucharest, Romania.
authors.forename: Alexandru
authors.initials: A
authors.lastname: Babes
authors.forename: Bogdan
authors.initials: B
authors.lastname: Amuzescu
authors.forename: Ulrich
authors.initials: U
authors.lastname: Krause
authors.forename: Andreas
authors.initials: A
authors.lastname: Scholz
authors.forename: Maria Luiza
authors.initials: ML
authors.lastname: Flonta
authors.forename: Gordon
authors.initials: G
authors.lastname: Reid
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D002478:Cells, Cultured; D003080:Cold Temperature; D017679:Cryotherapy; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007036:Hypothermia, Induced; D007249:Inflammation / Q000378:metabolism* / Q000503:physiopathology; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009619:Nociceptors / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D059408:Pain Management; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*; D050916:TRPV Cation Channels
chemical_list: D011955:Receptors, Drug; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002211:Capsaicin