Detection, distribution and selection of microsatellites (SSRs) in the genome of the yeast Saccharomyces cerevisiae as molecular markers.
OBJECTIVE
The aim of this work was the selection of six polymorphic microsatellite loci for their use as molecular markers in the identification, typification and genetic differentiation of S. cerevisiae strains.


RESULTS
The selection was undertaken following a search of the genomic DNA database of Saccharomyces cerevisiae for simple tandem repeat sequences (microsatellites) of di- and trinucleotides. The genetic variability generated by these markers was evaluated in 51 isolates. The discriminatory power produced by combining the information obtained by the six microsatellites was very high. A total of 57 alleles, which generated 44 genotypes, were found.


CONCLUSIONS
The multiple analysis of microsatellites proved to be a powerful and agile tool for analysing the genome of S. cerevisiae populations.
Pérez
M A
MA
Instituto Madrileño de Investigación Agraria y Alimentaria (IMIA.), Comunidad de Madrid, Finca El Encín, Apdo. 127, 28800 Alcalá de Henares, Madrid. Spain.
Gallego
F J
FJ
Martínez
I
I
Hidalgo
P
P
D000483:Alleles; D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000032:analysis*; D005787:Gene Frequency; D014644:Genetic Variation; D016681:Genome, Fungal; D005838:Genotype; D018895:Microsatellite Repeats; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics*; D014920:Wine / Q000382:microbiology
D004271:DNA, Fungal