Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells.
Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 microM, but not dose-dependent from 0.1 to 20 microM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.
Shibuya
Koichi
K
Department of Medical Radioscience, Faculty of Health Sciences, Okayama University Medical School, Okayama 700-8558, Japan. kshibuya@md.okayama-u.ac.jp
Kawasaki
Shoji
S
Kuroda
Masahiro
M
Takemoto
Mitsuhiro
M
Asaumi
Jun-Ichi
J
Hiraki
Yoshio
Y
D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006358:Hot Temperature / Q000009:adverse effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008647:Mesocricetus; D009183:Mycotoxins / Q000494:pharmacology; D000081082:Phosphoinositide-3 Kinase Inhibitors; D013312:Stress, Physiological; D000077191:Wortmannin
D000730:Androstadienes; D004791:Enzyme Inhibitors; D009183:Mycotoxins; D000081082:Phosphoinositide-3 Kinase Inhibitors; D000077191:Wortmannin