title: Comparative detection of measles and rubella IgM and IgG derived from filter paper blood and serum samples.
abstract: We compared the use of serum and filter paper blood spots as specimen sources for the detection of measles- and rubella-specific IgM and IgG. We collected capillary blood into microtainer tubes and onto filter paper spots from 60 children and 60 healthy adults. The blood was collected from 12-15-month-old children approximately 3 weeks after primary vaccination with measles, mumps, rubella vaccine, and the sample-pairs were tested for measles-specific IgM and IgG antibodies by using a capture antibody EIA and an indirect EIA, respectively. We tested sample-pairs from a subset of participants for rubella- specific IgM and IgG antibodies by using commercially available capture IgM (Captia) and indirect IgG (Wampole) assays. The concordance of results from serum and filter paper blood spots was high for all assays: 98% for measles IgM, 93% for measles IgG, 94% for rubella IgM, and 93% for rubella IgG, and increased to between 96-100% for all four assays when indeterminate samples were excluded. The correlation coefficients for EIA signals were 0.99 and 0.77 for measles IgM and IgG, respectively, and 0.92 and 0.94 for rubella IgM and IgG, respectively. The cut-off values used for filter paper samples were the same as those used for serum samples for all tests except for the rubella IgM assay. The use of filter paper blood spots is a promising future option for the detection of measles- and rubella-specific antibodies.
authors.affiliation: Respiratory and Enteric Viruses Branch, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30333, USA. rhelfand@cdc.gov
authors.forename: R F
authors.initials: RF
authors.lastname: Helfand
authors.forename: H L
authors.initials: HL
authors.lastname: Keyserling
authors.forename: I
authors.initials: I
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Murray
authors.forename: J
authors.initials: J
authors.lastname: Mei
authors.forename: C
authors.initials: C
authors.lastname: Moscatiello
authors.forename: J
authors.initials: J
authors.lastname: Icenogle
authors.forename: W J
authors.initials: WJ
authors.lastname: Bellini
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D001800:Blood Specimen Collection; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007223:Infant; D008297:Male; D008459:Measles virus / Q000276:immunology*; D008875:Middle Aged; D010209:Paper; D012412:Rubella virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C040901:rubella antibodies