[Enterotoxin-producing Bacteroides fragilis strains isolated from horses].
Seven Bacteroides fragilis strains were cultured from samples collected from horses. From all the tested strains, as well as from the reference B. fragilis strains: enterotoxigenic NCTC 11925 and nonenterotoxigenic IPL 323 strain, DNA was isolated using Genomic DNA PREP PLUS isolation kit manufactured by A&A Biotechnology (Poland). To detect the enterotoxin (fragilysin) gene, polymerase chain reaction (PCR) was applied, using the following starters: 404 (GAG CCG AAG ACG GTG TAT GTG ATT TGT) and 407 (TGC TCA GCG CCC AGT ATA TGA CCT AGT). DNA obtained from bacterial cells was amplified in a thermocycler (Techne). The temperature profile was as follows: 1 cycle (4 min. 94 degrees C), 40 cycles (1 min. 94 degrees C, 1 min. 52 degrees C, 1 min. 74 degrees C). Amplification products were detected by electrophoresis in agarose gel (1%) with ethidium bromide added. The presence of the fragilysin gene was detected in two strains. Among the strains isolated from horses enterotoxin gene-possessing Bacteroides fragilis strains (ETBF) can be detected.
Obuch-Woszczatyński
P
P
Katedra i Zakład Mikrobiologii Lekarskiej Centrum Biostruktury, Akademia Medyczna w Warszawie.
Pituch
H
H
Martirosian
G
G
Silva
J
J
Meisel-Mikołajczyk
F
F
Łuczak
M
M
D000818:Animals; D001441:Bacteroides fragilis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001842:Bone and Bones / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D004768:Enterotoxins / Q000096:biosynthesis*; D006736:Horses; D007422:Intestines / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008666:Metalloendopeptidases / Q000032:analysis; D013710:Tendons / Q000382:microbiology
D004269:DNA, Bacterial; D004768:Enterotoxins; D008666:Metalloendopeptidases; C091198:fragilysin