Calcineurin-dependent mitochondrial disturbances in calcium-induced apoptosis of human immunodeficiency virus gp160-expressing CD4+ cells.
In CD4+ UE160 cells with inducible expression of gp160, mechanisms of apoptosis induced by human immunodeficiency virus (HIV) Env protein were analyzed. Induction of gp160 caused intracellular calcium increase followed by the release of cytochrome c from mitochondria, which was inhibited by calcineurin inhibitors. Association of BAD with Bcl-xL was observed, and a portion of BAD was dephosphorylated after induction of gp160. These data suggested that calcineurin plays a role in the HIV Env-induced apoptosis in a mitochondrion-dependent way.
Sasaki
Masafumi
M
Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
Miyazaki
Kozo
K
Koga
Yasuhiro
Y
Kimura
Genki
G
Nomoto
Kikuo
K
Yoshida
Hiroki
H
D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019256:Cadmium Chloride / Q000494:pharmacology; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D020298:U937 Cells
D019302:HIV Envelope Protein gp160; D019703:Calcineurin; D019256:Cadmium Chloride; D002118:Calcium