VmrA, a member of a novel class of Na(+)-coupled multidrug efflux pumps from Vibrio parahaemolyticus.
Gene vmrA, cloned from Vibrio parahaemolyticus, made Escherichia coli resistant to 4',6-diamino-2-phenylindol, tetraphenylphosphonium chloride, acriflavine, and ethidium bromide. VmrA belongs to the DinF branch of MATE family efflux transporters. VmrA catalyzed acriflavine efflux and showed Na(+)/drug transporter activity because the addition of tetraphenylphosphonium to Na(+)-loaded cells caused Na(+) efflux.
Chen
Jing
J
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Morita
Yuji
Y
Huda
M Nazmul
MN
Kuroda
Teruo
T
Mizushima
Tohru
T
Tsuchiya
Tomofusa
T
D000167:Acriflavine / Q000378:metabolism; D000891:Anti-Infective Agents, Local / Q000378:metabolism; D017920:Antiporters / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D000254:Sodium-Potassium-Exchanging ATPase / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D000891:Anti-Infective Agents, Local; D017920:Antiporters; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; C445308:VmrA protein, Vibrio parahaemolyticus; D000167:Acriflavine; D000254:Sodium-Potassium-Exchanging ATPase