Response of MCF-7 human breast cancer cells to some binary mixtures of oestrogenic compounds in-vitro.
The effects of simultaneous administration of some binary mixtures of seven natural and synthetic oestrogenic substances (17beta-estradiol, estrone, bisphenol A, butylbenzyl phthalate, endosulfan, methoxychlor and pentachlorophenol) on the cellular proliferation of human breast cancer MCF-7 cells in-vitro (a modified E-screen assay) have been measured. To assess the presence or absence of interactions of the two agents, the data were analysed on the basis of a graphical method in which the types and extents of interactions were described by response-surface diagrams. Of the nine combinations of the agents examined, synergistic interaction was evident for the combination of 17beta-estradiol and bisphenol A, whereas the remaining eight combinations were weakly synergistic, additive and/or weakly antagonistic in the dose-range tested.
Suzuki
T
T
Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan. tsuzuki@res.titech.ac.jp
Ide
K
K
Ishida
M
M
D001559:Benzhydryl Compounds; D001943:Breast Neoplasms / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004726:Endosulfan / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism*; D004970:Estrone / Q000494:pharmacology; D006801:Humans; D008731:Methoxychlor / Q000494:pharmacology; D008954:Models, Biological; D010416:Pentachlorophenol / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D010795:Phthalic Acids / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004726:Endosulfan; D004958:Estradiol; D004967:Estrogens; D004970:Estrone; D008731:Methoxychlor; D010416:Pentachlorophenol; D010636:Phenols; D010795:Phthalic Acids; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C027561:butylbenzyl phthalate