title: Determinants of the phagosomal pH in neutrophils.
abstract: Phagosomes formed by neutrophils are much less acidic than those of other phagocytic cells. The defective acidification seen in neutrophils has been attributed to consumption of protons during the dismutation of superoxide, because a large, sustained acidification is unmasked when the cells are treated with inhibitors of the NADPH oxidase. Consumption of protons transported into the phagosome by dismutation would tightly couple the activities of the NADPH oxidase and the vacuolar type H(+)-pump (or V-ATPase). We tested the existence of the predicted coupling using microfluorimetry and digital imaging and found that the rate of superoxide generation was independent of the activity of the H(+)-pump. Moreover, we failed to detect the alkalinization predicted to develop through dismutation when the pump was inhibited. Instead, two other mechanisms were found to contribute to the inability of neutrophil phagosomes to acidify. First, the insertion of V-ATPases into the phagosomal membrane was found to be reduced when the oxidase is active. Second, the passive proton (equivalent) permeability of the phagosomal membrane increased when the oxidase was activated. The increased permeability cannot be entirely attributed to the conductive H(+) channels associated with the oxidase, since it is not eliminated by Zn(2+). We conclude that the NADPH oxidase controls the phagosomal pH by multiple mechanisms that include reduced proton delivery to the lumen, increased luminal proton consumption, and enhanced backflux (leak) into the cytosol.
authors.affiliation: Cell Biology Programme, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Jankowski
authors.forename: Cameron C
authors.initials: CC
authors.lastname: Scott
authors.forename: Sergio
authors.initials: S
authors.lastname: Grinstein
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D018942:Macrolides; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000097:blood; D009504:Neutrophils / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D010588:Phagosomes / Q000502:physiology*; D014634:Valinomycin / Q000494:pharmacology; D015054:Zymosan / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D018942:Macrolides; D014634:Valinomycin; C036978:concanamycin A; D015054:Zymosan; D019255:NADPH Oxidases