The phytochrome A specific signaling component PAT3 is a positive regulator of Arabidopsis photomorphogenesis.
Phytochrome A plays a major role in early seedling development by triggering the transition from etiolated growth to greening. Seedlings germinated under constant far-red (FR) light show a partially de-etiolated phenotype that is not seen in phyA mutants. This phytochrome A specific response was used to screen a population of T-DNA mutagenized Arabidopsis seedlings. One mutant line, pat3 (phytochrome A signal transduction3), which showed no inhibition of hypocotyl elongation under FR light conditions and no FR-induced killing response, contained a T-DNA insertion in a 609-bp ORF. The recessive mutation co-segregated with the T-DNA resistance marker and could be allelic to fhy1. A 2,248-bp genomic fragment of the PAT3 locus can complement the pat3 mutant phenotype. PAT3 transcript peaked 3 d after germination and was downregulated by light. PAT3 has no significant homology to any known protein and shows no preferential cellular localization. The protein can activate transcription in yeast when fused to the GAL4 DNA-binding domain. Our results show that PAT3 is a positive regulator of phytochrome A signal transduction.
Zeidler
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Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
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D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D010834:Phytochrome / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D051942:Phytochrome A; D015398:Signal Transduction
D029681:Arabidopsis Proteins; C441269:FHY1 protein, Arabidopsis; C497973:PHYA protein, Arabidopsis; D051942:Phytochrome A; D010834:Phytochrome