title: Oxidized plant sterols in human serum and lipid infusions as measured by combined gas-liquid chromatography-mass spectrometry.
abstract: Some oxidized forms of cholesterol (oxysterols) are thought to be atherogenic and cytotoxic. Because plant sterols are structurally related to cholesterol, we examined whether oxidized plant sterols (oxyphytosterols) could be identified in human serum and soy-based lipid emulsions. We first prepared both deuterated and nondeuterated reference compounds. We then analyzed by gas-liquid chromatography-mass spectrometry the oxyphytosterol concentrations in serum from patients with phytosterolemia or cerebrotendinous xanthomatosis, in a pool serum and in two lipid emulsions. 7-Ketositosterol, 7 beta-hydroxysitosterol, 5 alpha, 6 alpha-epoxysitosterol, 3 beta,5 alpha,6 beta-sitostanetriol, and probably also 7 alpha-hydroxysitosterol were present in markedly elevated concentrations in serum from phytosterolemic patients only. Also, campesterol oxidation products such as 7 alpha-hydroxycampesterol and 7 beta-hydroxycampesterol were found. Interestingly, sitosterol was oxidized for approximately 1.4% in phytosterolemic serum, which is rather high compared with the approximate 0.01% oxidatively modified cholesterol normally seen in human serum. The same oxyphytosterols were also found in two lipid emulsions in which the ratio of oxidized sitosterol to sitosterol varied between 0.038 and 0.041. In conclusion, we have shown that oxidized forms of plant sterols are present in serum from phytosterolemic patients and two frequently used soy-based lipid emulsions. Currently, it is unknown whether oxyphytosterols affect health, as has been suggested for oxysterols. However, 7 beta-hydroxycholesterol may be one of the more harmful oxysterols, and both sitosterol and campesterol were oxidized into 7 beta-hydroxysitosterol and 7 beta-hydroxycampesterol. The relevance of these findings therefore deserves further exploration.
authors.affiliation: Maastricht University, Department of Human Biology, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. J.Plat@HB.UNIMAAS.NL
authors.forename: J
authors.initials: J
authors.lastname: Plat
authors.forename: H
authors.initials: H
authors.lastname: Brzezinka
authors.forename: D
authors.initials: D
authors.lastname: Lütjohann
authors.forename: R P
authors.initials: RP
authors.lastname: Mensink
authors.forename: K
authors.initials: K
authors.lastname: von Bergmann
mesh_terms: D002648:Child; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000097:blood*; D010084:Oxidation-Reduction; D010840:Phytosterols / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008055:Lipids; D010840:Phytosterols; D002784:Cholesterol