title: Determination of scopolamine, atropine and anisodamine in Flos daturae by capillary electrophoresis.
abstract: A capillary electrophoresis method was developed for the separation and determination of tropane alkaloids in Flos daturae plants. Separation was performed on a fused silica capillary(42.1 cm x 50 microm i.d.) at an applied voltage of 20 kV. Scopolamine, atropine and anisodamine were well separated in the buffer of 50 mmol/L phosphate buffer (pH 5.0) containing 20% (v/v) tetrahydrofuran (THF). Beer's law was obeyed in the range of concentration of 2.4-21.8 microg/mL for scopolamine, 4.0-36.0 microg/mL for atropine and 2.6-23.7 microg/mL for anisodamine, respectively, and the correlation coefficients were over 0.999 (n = 6). The developed method was applied for the analysis of herb samples.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100037, People's Republic of China.
authors.forename: N
authors.initials: N
authors.lastname: Ye
authors.forename: R
authors.initials: R
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Gu
authors.forename: H
authors.initials: H
authors.lastname: Zou
mesh_terms: D001285:Atropine / Q000032:analysis*; D002021:Buffers; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005663:Furans; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D015203:Reproducibility of Results; D012601:Scopolamine / Q000032:analysis*; D012991:Solanaceous Alkaloids / Q000032:analysis*
chemical_list: D002021:Buffers; D004365:Drugs, Chinese Herbal; D005663:Furans; D012991:Solanaceous Alkaloids; C003922:anisodamine; C018674:tetrahydrofuran; D001285:Atropine; D012601:Scopolamine