title: Kinetic locking-on strategy and auxiliary tactics for bioaffinity purification of NAD(P)(+)-dependent dehydrogenases.
authors.affiliation: Department of Applied Biology and Chemistry, Institute of Technology Carlow, Kilkenny Road, Carlow, Ireland.
authors.forename: P
authors.initials: P
authors.lastname: Mulcahy
authors.forename: M
authors.initials: M
authors.lastname: O'Flaherty
mesh_terms: D000426:Alcohol Dehydrogenase / Q000302:isolation & purification; D000818:Animals; D002175:Candida / Q000201:enzymology; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification; D006736:Horses; D019811:Hydroxylamine; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009243:NAD / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D009243:NAD; D019811:Hydroxylamine; D000426:Alcohol Dehydrogenase; D005969:Glutamate Dehydrogenase; C061421:glutamate dehydrogenase (NAD(P)+); D009252:NADPH Dehydrogenase