A factor isolated from Taenia solium metacestodes stimulates T lymphocytes to proliferate and produce gamma interferon.
A metacestode factor (MF) isolated from live metacestodes of Taenia solium suppresses humoral and cellular responses, and inhibits the inflammatory reaction around metacestodes implanted subcutaneously in mice. When this MF is digested with RNase (dMF), it loses the suppressive capacity, but acquires T-cell stimulant ability. By filtering MF through a Bio-gel P6 column, two components were separated. The first (F1) was suppressive. while the second (F2) stimulated T cells to proliferate. In these experiments, F2 or dMF was used with mouse spleen cells in stimulation assays in vitro. Spleen cells from mice treated with F2 or dMF were also stimulated with concanavalin A (Con-A) ex vivo. Flow cytometry analyses were performed to estimate cell proliferation, intracellular cytokine production. and restoration of CD4 cells. Spleen lymphocytes from mice previously treated with F2 or dMF and then stimulated with Con-A ex vivo exhibited a significant increase in cell proliferation and gamma interferon production by CD4+ (P<0.05) and CD8+ cells. These effects were concentration-dependent and inversely correlated with the amount of dMF or F2. Similar results were observed in normal mouse spleen T cells incubated with F2 or dMF and Con-A in vitro. Finally, dMF induced a significant restoration of CD4-cells in mice depleted of these cells.
Garrido
E
E
Department of Molecular Genetics, Institute of Cellular Physiology, UNAM, Mexico City, Mexico DF.
Tato
P
P
Molinari
J L
JL
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D015801:Helminth Proteins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D006790:Host-Parasite Interactions; D007371:Interferon-gamma / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology*; D012260:Ribonucleases / Q000378:metabolism; D012480:Salmonella Infections / Q000276:immunology / Q000401:mortality; D012486:Salmonella typhimurium; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D015996:Survival Rate; D013552:Swine; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013621:Taenia / Q000737:chemistry / Q000378:metabolism*
D015801:Helminth Proteins; D008934:Mitogens; D007371:Interferon-gamma; D012260:Ribonucleases