Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes.
HP1 proteins are conserved non histone chromosomal proteins involved in the epigenetic repression of transcription. Three HP1 proteins, HP1alpha, HP1beta and HP1gamma are expressed in mammalian cells. Polyclonal antibodies directed against peptides specific for HP1alpha and HP1gamma were elicited in rabbits, affinity purified, then used to localize both proteins on spreads of unfixed metaphasic chromosomes. We show here, by conventional and confocal microscopy, that both proteins are localized at centromeres and pericentromeres, and that HP1gamma is also present on euchromatic sites of chromosome arms.
Minc
E
E
Département de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universitès Paris 6 et 7, Paris, France.
Allory
Y
Y
Courvalin
J C
JC
Buendia
B
B
D002503:Centromere / Q000648:ultrastructure*; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008677:Metaphase
C000715593:CBX1 protein, human; C000715594:CBX5 protein, human; D002868:Chromosomal Proteins, Non-Histone; D000090266:Chromobox Protein Homolog 5