Selective localization of G protein gamma5 subunit in the subventricular zone of the lateral ventricle and rostral migratory stream of the adult rat brain.
G proteins play important roles in transmembrane signal transduction, and various isoforms of each subunit, alpha, beta and gamma, are highly expressed in the brain. The Ggamma5 subunit is a minor isoform in the adult brain, but we have previously shown it to be highly expressed in the proliferative region of the ventricular zone in the rat embryonic brain. We show here that Ggamma5 is also selectively localized in a proliferative region in the adult rat brain, including the subventricular zone of the lateral ventricle and rostral migratory stream. The Galphai2 subunit colocalized with Ggamma5 in these regions, the two subunits being present in neuronal precursors and ependymal cells but not in proliferating astrocytes. In addition, intense staining of Ggamma5 was seen in axons of the olfactory neurons, which are known to regenerate. These results suggest specific roles for Ggamma5 in precursor cells during neurogenesis so that this isoform might be a useful biological marker.
Asano
T
T
Institute for Developmental Research, Aichi Human Service Center, Kasugai, Aichi, Japan. tosano@inst-hsc.pref.aichi.jp
Shinohara
H
H
Morishita
R
R
Ueda
H
H
Kawamura
N
N
Katoh-Semba
R
R
Kishikawa
M
M
Kato
K
K
D000818:Animals; D001369:Axons / Q000737:chemistry; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D004805:Ependyma / Q000737:chemistry* / Q000166:cytology; D005865:Gestational Age; D020962:Heterotrimeric GTP-Binding Proteins / Q000032:analysis*; D007124:Immunoenzyme Techniques; D007395:Interneurons / Q000737:chemistry* / Q000166:cytology; D020547:Lateral Ventricles / Q000737:chemistry* / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009830:Olfactory Bulb / Q000737:chemistry* / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D020033:Protein Isoforms / Q000032:analysis*; D021122:Protein Subunits; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000737:chemistry* / Q000166:cytology
D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D021122:Protein Subunits; D020962:Heterotrimeric GTP-Binding Proteins; D001973:Bromodeoxyuridine