title: Effect of dopamine depletion on DARPP-32 protein in ischemic rat striatum.
abstract: OBJECTIVE
  To study the effects of dopamine depletion on the phosphorylation level, intracellular distribution, and mRNA expression of DARPP-32 in the ischemic striatum and to elucidate the mechanisms underlying the ischemic injury.
  
  
  METHODS
  A complex model of SN lesioning with 6-OHDA to deplete dopamine and four vessels occlusion for inducing forebrain ischemia was constructed in rats. DARPP-32 was investigated with autoradiogram, immunohistochemistry and in situ hybridization.
  
  
  RESULTS
  The [32P]phosphate incorporation of DARPP-32 was reduced in vitro following ischemia. However, the [32P]phosphate incorporation, the numbers of positive neurons, and mRNA expression of DARPP-32 were increased in SN lesioning plus ischemic rats with denervated striatum.
  
  
  CONCLUSIONS
  Dopamine depletion reduced the DARPP-32 phosphorylation in vivo following ischemia, and protected DARPP-32 immunoreactivity and mRNA expression level against the reduction induced by ischemia.
authors.affiliation: Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Sun
authors.forename: F M
authors.initials: FM
authors.lastname: Tang
authors.forename: Y M
authors.initials: YM
authors.lastname: Ding
authors.forename: Y T
authors.initials: YT
authors.lastname: Chen
authors.forename: G Y
authors.initials: GY
authors.lastname: Zhang
authors.forename: G Z
authors.initials: GZ
authors.lastname: Jin
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000139:chemically induced / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D008297:Male; D009419:Nerve Tissue Proteins; D016627:Oxidopamine; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D016627:Oxidopamine; D004298:Dopamine