Optical imaging of excitation propagation evoked by stimulation to the trigeminal caudalis.
The present study reports on a characteristic spatio-temporal propagation of differential fluorescent images in the rat brain stem slice by a high-speed optical imaging technique. Coronal or sagittal slices were stained with a voltage-sensitive dye RH-414, and the superficial layer of the trigeminal caudalis (Vc) was then electrically stimulated. The marginal layer and substantia gelatinosa showed larger rostrocaudal excitation than coronal, despite a lack of tract stimulation expansion in either direction. A perfusion of 0.5 micromol/l TTX, not 10 micromol/l CNQX suppressed these propagations. These findings suggest that the superficial layer of Vc has spatial differences in neuronal excitation propagation, as evidenced by morphological observations that dendrites in the superficial layers extend in the rostrocaudal direction.
Seo
K
K
Division of Dental Anesthesiology, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 5274, 2 Ban-cho, Gakkocho-dori, Niigata, 951-8514, Japan.
Fujiwara
N
N
Takeuchi
K
K
Maeda
T
T
Someya
G
G
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008856:Microscopy, Fluorescence; D009434:Neural Pathways; D009619:Nociceptors / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013376:Substantia Gelatinosa / Q000166:cytology* / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014275:Trigeminal Caudal Nucleus / Q000166:cytology* / Q000502:physiology*
D000779:Anesthetics, Local; D018691:Excitatory Amino Acid Antagonists; D013779:Tetrodotoxin; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione