SRC1: an intron-containing yeast gene involved in sister chromatid segregation.
Analysis of a three-member gene family in the yeast Saccharomyces cerevisiae has allowed the discovery of a new gene that comprises two contiguous open reading frames previously annotated as YML034w and YML033w. The gene contains a small intron with two alternative 5' splicing sites. It is specifically transcribed during G(2)/M in the cell cycle and after several hours of meiosis induction. Splicing of the mRNA is partially dependent on NAM8 but does not vary during meiosis or the cell cycle. Deletion of the gene induces a shortening of the anaphase and aggravates the phenotype of scc1 and esp1 conditional mutants, which suggests a direct role of the protein in sister chromatid separation.
Rodríguez-Navarro
Susana
S
Departamento de Bioquímica y Biología Molecular, Universitat de València, C/ Dr. Moliner 50, E-46100 Burjassot, Spain.
Igual
J Carlos
JC
Pérez-Ortín
José E
JE
D020121:5' Untranslated Regions / Q000235:genetics; D017398:Alternative Splicing; D001483:Base Sequence; D017353:Gene Deletion; D005800:Genes, Fungal; D007438:Introns; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012854:Sister Chromatid Exchange
D020121:5' Untranslated Regions