Differential requirement for SLP-76 domains in T cell development and function.
The hematopoietic cell-specific adaptor protein, SLP-76, is critical for T cell development and mature T cell receptor (TCR) signaling; however, the structural requirements of SLP-76 for mediating thymopoiesis and mature T cell function remain largely unknown. In this study, transgenic mice were generated to examine the requirements for specific domains of SLP-76 in thymocytes and peripheral T cells in vivo. Examination of mice expressing various mutants of SLP-76 on the null background demonstrates a differential requirement for specific domains of SLP-76 in thymocytes and T cells and provides new insight into the molecular mechanisms underlying SLP-76 function.
Myung
P S
PS
Graduate Program in Immunology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Derimanov
G S
GS
Jordan
M S
MS
Punt
J A
JA
Liu
Q H
QH
Judd
B A
BA
Meyers
E E
EE
Sigmund
C D
CD
Freedman
B D
BD
Koretzky
G A
GA
D048868:Adaptor Proteins, Signal Transducing; D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D017252:CD3 Complex / Q000276:immunology; D020013:Calcium Signaling; D002352:Carrier Proteins / Q000502:physiology; D002454:Cell Differentiation; D017637:Clonal Deletion / Q000502:physiology; D016130:Immunophenotyping; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D020125:Mutation, Missense; D010750:Phosphoproteins / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000502:physiology; D017384:Sequence Deletion; D015398:Signal Transduction / Q000502:physiology; D013154:Spleen / Q000276:immunology; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D017252:CD3 Complex; D002352:Carrier Proteins; C491734:Lat protein, mouse; D008565:Membrane Proteins; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; C092947:SLP-76 signal Transducing adaptor proteins