Protein-DNA interaction and CpG methylation at rep*/vIL-10p of latent Epstein-Barr virus genomes in lymphoid cell lines.
The viral interleukin-10 promoter (vIL-10p), overlapping the rep* element in the Epstein-Barr virus (EBV) genome, is a promoter element active mostly in the late phase of the lytic cycle and immediately upon infection of B cells. rep* was, through transfection experiments with small plasmids, characterised as a cis element supporting oriP replicative function. In this study, in vivo protein binding and CpG methylation at rep*/vIL-10p were analysed in five cell lines that harbour strictly latent EBV genomes. Contrary to the invariably unmethylated dyad symmetry element (DS) of oriP, rep*/vIL-10p was highly methylated and showed only traces of protein binding in all examined cell lines. This result is in agreement with vIL-10p being an inactive promoter of EBV genomes, and makes it less likely that rep* functions as a replicative element of latent EBV genomes.
Niller
H H
HH
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany.
Salamon
D
D
Takacs
M
M
Uhlig
J
J
Wolf
H
H
Minarovits
J
J
D001483:Base Sequence; D002460:Cell Line; D018899:CpG Islands; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008214:Lymphocytes / Q000166:cytology / Q000821:virology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics
D011506:Proteins; D016753:Interleukin-10; D004247:DNA