4-Hydroxybenzoate uptake in Klebsiella planticola strain DSZ1 is driven by DeltapH.
Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 x 10-6 (cpm/OD) h-1, and a rate of 4-HBA utilisation of 0.75 mmol h-1. Aerobically the 4HBA transport system is driven by gradient of protons (DeltapH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 microM with a Vmax value of 25 nmol/min.mg at pH 7.
Allende
J L
JL
Dpt. Fisica Aplicada, Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, Spain.
Gibello
A
A
Fortún
A
A
Sánchez
M
M
Martín
M
M
D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D001565:Benzoates / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D010226:Parabens / Q000378:metabolism*; D019810:Sodium Azide / Q000378:metabolism
D001565:Benzoates; D010226:Parabens; D000255:Adenosine Triphosphate; D019810:Sodium Azide