title: Effects of erysimin G on renal tubular function and 70-pS K+ channel activity of thick ascending limb.
abstract: OBJECTIVE
  To study the effect of erysimin G (CH35H52O13) on the thick ascending limb (TAL) 70-pS K+ channel of rat kidney and its effect on diuresis.
  
  
  METHODS
  The patch-clamp cell-attached recording technique was used to record the single potassium channel current, and the urine volume (UV) was measured by urethral intubation to determine the diuretic effect.
  
  
  RESULTS
  Erysimin G can increase the urine volume and decrease the 70-pS potassium channel activity of TAL.
  
  
  CONCLUSIONS
  Erysimin G has a diuretic effect and its inhibition on the activity of apical 70-pS potassium channel may be the mechanism of its diuretic effect.
authors.affiliation: Department of Pharmacology, Harbin Medical University, Harbin 150086, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Shan
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
authors.forename: R M
authors.initials: RM
authors.lastname: Gu
authors.forename: D L
authors.initials: DL
authors.lastname: Luo
authors.forename: B F
authors.initials: BF
authors.lastname: Yang
mesh_terms: D000818:Animals; D002301:Cardiac Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D004232:Diuretics / Q000302:isolation & purification / Q000494:pharmacology*; D032601:Erysimum / Q000737:chemistry; D005260:Female; D007686:Kidney Tubules, Distal / Q000502:physiology*; D008297:Male; D018408:Patch-Clamp Techniques; D010946:Plants, Medicinal / Q000737:chemistry; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D014556:Urine
chemical_list: D002301:Cardiac Glycosides; D004232:Diuretics; D015221:Potassium Channels; C478419:erysimin G