Pharmacokinetics of lactosaminated recombinant human growth hormone in mice.
OBJECTIVE
To study the pharmacokinetic characteristics of lactosaminated recombinant human growth hormone (hGH-L) in mice.


METHODS
The biodistribution was studied with in vivo radioactive tracer technique. The pharmacokinetics was investigated by radioimmunoassay (RIA) method of hGH-L. The results were compared with that of recombinant human growth hormone (hGH).


RESULTS
125I-hGH-L has remarkable livertaxis. The area under drug concentration-time curve (32686.9 microg . min . L-1) in blood and serum mean residence time (21.4 min) of hGH-L are less than that of hGH (36913.1 microg . min . L-1 and 24.9 min) (P < 0.05). In target organ liver, hGH-L distribution half life (1.8 min) and elimination half life (11.1 min) are shorter than that of hGH (2.1 min and 27.7 min) (P < 0.05). The area under drug concentration-time curve (17621.9 microg . min . L-1) of hGH-L is bigger than that of hGH(12148.2 microg . min . L-1) (P < 0.05) in liver.


CONCLUSIONS
The pharmacokinetic parameters of hGH-L has obvious advantage over that of hGH.
Li
Y C
YC
Department of Nuclear Medicine, the First Affiliated Hospital, West China Medicine Center, Sichuan University, Chengdu 610041, China. dnmwcums@sichuan-China.gov.cn
Guan
C T
CT
Zhao
K Q
KQ
Chen
Z L
ZL
Li
T L
TL
D000606:Amino Sugars / Q000378:metabolism*; D000818:Animals; D013006:Growth Hormone / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
D000606:Amino Sugars; D011994:Recombinant Proteins; C032233:lactosamine; D013006:Growth Hormone