WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa serogroup O11.
Lipopolysaccharide from a wbjA mutant, deficient in a putative glycosyltransferase from Pseudomonas aeruginosa serogroup O11, was compared to that from an O-antigen polymerase mutant. Results suggest that WbjA adds the terminal glucose to complete the serogroup O11 O-antigen unit and identifies the biological repeating unit as [-2)-beta-D-glucose-(1-3)-alpha-L-N-acetylfucosamine-(1-3)-beta-D-N-acetylfucosamine-(1].
Dean
Charles R
CR
Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.
Datta
Anup
A
Carlson
Russell W
RW
Goldberg
Joanna B
JB
D001426:Bacterial Proteins; D002240:Carbohydrate Sequence; D005947:Glucose / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000276:immunology / Q000378:metabolism*; D017421:Sequence Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D019081:O Antigens; C443988:Pseudomonas aeruginosa O11 antigen; D005964:Glucosyltransferases; C443989:WbjA protein, Pseudomonas aeruginosa; D005947:Glucose