title: Biological activities of a specific ecdysteroid dimer and of selected monomeric structural analogues in the B(II) bioassay.
abstract: The biological activities of selected specific ecdysteroids obtained by photochemical or chemical transformation are compared in the B(II) bioassay, in which the potency reflects the affinity of binding to the ligand-binding site of the Drosophila melanogaster ecdysteroid receptor. The compounds tested represent 14-deoxy, 14-dehydroxy, 14-hydroperoxy and 14-epi derivatives of 20-hydroxyecdysone and were selected on the basis of their close structural relationship to elucidate the contribution of the 14-hydroxy group and the stereochemical configuration at C-14 to ecdysteroid agonist activity. The structure-activity relationship shows that a 14-hydroxy group is not required for activity. However, the alpha-configuration of -H, -OH or -OOH at C-14, which determines the C/D rings trans-annelation, is very significant for activity; it is as important for activity as the well studied A/B rings cis-annelation. Compounds containing a double bond involving C-14 showed low activity with the exception of the specific, and so far unique, ecdysteroid dimer 7,7'-bis-[14-deoxy-8(14)-ene-20-hydroxyecdysone], which was obtained as the main product of the photochemical transformation of 20-hydroxyecdysone. The relatively high biological activity of this dimeric compound is discussed.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Flemingovo nám. 2, 166 10 Prague, Czech Republic. harmatha@uochb.cas.cz
authors.forename: Juraj
authors.initials: J
authors.lastname: Harmatha
authors.forename: Laurence
authors.initials: L
authors.lastname: Dinan
authors.forename: René
authors.initials: R
authors.lastname: Lafont
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D019281:Dimerization; D004331:Drosophila melanogaster; D004441:Ecdysterone / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D011987:Receptors, Steroid / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D011987:Receptors, Steroid; C034808:ecdysteroid receptor; D004441:Ecdysterone