title: Crystallization and preliminary crystallographic analysis of a metastasis-inducing protein, human S100A4.
abstract: A metastasis-inducing protein, human S100A4, cDNA was overexpressed in Escherichia coli. The recombinant protein was purified by three chromatographic procedures. Crystals were obtained using polyethylene glycol as a precipitant. The native S100A4 crystals did not diffract sufficiently well for data collection, but the SeMet S100A4 crystal, grown using similar conditions as for the native, was found to be stable during exposure to X-rays and diffracted to 4 A resolution in-house. The crystal belongs to space group P6 or P3. The unit-cell parameters are a = b = 47.1, c = 175.6 A, alpha = beta = 90, gamma = 120 degrees. Complete data will be collected using synchrotron radiation.
authors.affiliation: Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Guozhen
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Mark
authors.initials: M
authors.lastname: Bartlam
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.forename: Roger
authors.initials: R
authors.lastname: Barraclough
authors.forename: Philip S
authors.initials: PS
authors.lastname: Rudland
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000737:chemistry*
chemical_list: D011994:Recombinant Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human