Expression of Ngn1, Ngn2, Cash1, Gsh2 and Sfrp1 in the developing chick telencephalon.
Patterning of the chick telencephalon has been debated, especially in regard to a ventral (subpallial) or dorsal (pallial) nature of the dorsal ventricular ridge (DVR). Here we report the expression patterns of chick homologues of molecules known to be involved in telencephalic patterning in other vertebrate species. We show here that the transcription factors Ngn1, Ngn2, Cash1, Gsh2 and the secreted frizzled related protein 1 (sfrp1), a wnt receptor, are expressed in characteristic telencephalic domains during chick development. At embryonic day 7 (E7) Ngn1 and Ngn2 are localized in the dorsal pallium and the DVR, similar to the region of Pax6 expression. In contrast, Gsh2 and Cash1 are restricted to the subpallium and some DVR cells. Interestingly Sfrp, a dorsoventral boundary marker in mouse telencephalon, is expressed between the DVR and subpallium. Gsh2 and Pax6 double-positive cells further characterize this boundary region in the developing chick telencephalon.
Frowein
Julia v
Jv
MPI for Neurobiology, Am Klopferspitz 18a, 82152 Munich-Martinsried, Germany.
Campbell
Kenneth
K
Götz
Magdalena
M
D000818:Animals; D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D019521:Body Patterning / Q000235:genetics; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D006023:Glycoproteins / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics*; D013045:Species Specificity; D013687:Telencephalon / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics*; D000099274:Frizzled-Related Proteins
D030161:Avian Proteins; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D018398:Homeodomain Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; D000099274:Frizzled-Related Proteins; C099107:CASH-1 protein, Gallus gallus; C109498:Gsh2 protein, mouse; C491183:NEUROG2 protein, human; C102938:Neurog2 protein, mouse; C486336:Neurog1 protein, mouse