Effect of D609 on phosphatidylcholine metabolism in the nuclei of LA-N-1 neuroblastoma cells: a key role for diacylglycerol.
In our previous studies, TPA treatment of LA-N-1 cells stimulated the production of diacylglycerol in nuclei, probably through the activation of a phospholipase C. Stimulation of the synthesis of nuclear phosphatidylcholine by the activation of CTP:phosphocholine cytidylyltransferase was also observed. The present data show that both effects were inhibited by the pretreatment of the cells with D609, a selective phosphatidylcholine-phospholipase C inhibitor, indicating that the diacylglycerol produced through the hydrolysis of phosphatidylcholine in the nuclei is reutilized for the synthesis of nuclear phosphatidylcholine and is required for the activation of CTP:phosphocholine cytidylyltransferase.
Antony
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P
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Farooqui
A A
AA
Horrocks
L A
LA
Freysz
L
L
D000975:Antioxidants / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism*; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism / Q000502:physiology*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D050356:Lipid Metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D009636:Norbornanes; D010713:Phosphatidylcholines / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism
D000975:Antioxidants; D001952:Bridged-Ring Compounds; D004075:Diglycerides; D004791:Enzyme Inhibitors; D009636:Norbornanes; D010713:Phosphatidylcholines; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D019916:Choline-Phosphate Cytidylyltransferase; D010738:Type C Phospholipases