Steep variation in mitochondrial DNA and B chromosomes among natural populations of Eyprepocnemis plorans (Acrididae).
Restriction enzyme analysis of the mitochondrial DNA of Eyprepocnemis plorans with 9 restriction enzymes revealed low variability--only EcoRI revealed any variability with 3 distinguishable digestion patterns, here named types I, II and III. The samples studied were collected from regions where different types of B chromosome exist. The demes from the central area show the B1 type (as a relict of a probably once continuous distribution) and also mtDNA type II. These show a parallel substitution towards the SW neighbouring demes by other Bs and mtDNA type I. However, the latter mtDNA type had a wider distribution and is predominant, or even the only one found in most other samples where other Bs exist. Considering the mtDNA as a marker unlinked with the Bs, some genetic differences should exist among areas defined by the B chromosomes. Our results support the hypothesis that in the central region the B chromosomes and the mtDNA are involved in two different events of substitution, but these do not necessarily occur in the same way.
Clemente
M
M
Unidad de Genética, Universidad San Pablo CEU, Montepríncipe, Boadilla del Monte, Madrid, Spain. clemente@dna.uba.ar
Garma
C
C
De Sola
B G
BG
Henriques-Gil
N
N
D000818:Animals; D002875:Chromosomes / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005843:Geography; D006110:Grasshoppers / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D013030:Spain
D004272:DNA, Mitochondrial