title: Spatial and temporal expression pattern of Runx3 (Aml2) and Runx1 (Aml1) indicates non-redundant functions during mouse embryogenesis.
abstract: The human RUNX3/AML2 gene belongs to the 'runt domain' family of transcription factors that act as gene expression regulators in major developmental pathways. Here, we describe the expression pattern of Runx3 during mouse embryogenesis compared to the expression pattern of Runx1. E10.5 and E14.5-E16.5 embryos were analyzed using both immunohistochemistry and beta-galactosidase activity of targeted Runx3 and Runx1 loci. We found that Runx3 expression overlapped with that of Runx1 in the hematopoietic system, whereas in sensory ganglia, epidermal appendages, and developing skeletal elements, their expression was confined to different compartments. These data provide new insights into the function of Runx3 and Runx1 in organogenesis and support the possibility that cross-regulation between them plays a role in embryogenesis.
authors.affiliation: Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Levanon
authors.forename: O
authors.initials: O
authors.lastname: Brenner
authors.forename: V
authors.initials: V
authors.lastname: Negreanu
authors.forename: D
authors.initials: D
authors.lastname: Bettoun
authors.forename: E
authors.initials: E
authors.lastname: Woolf
authors.forename: R
authors.initials: R
authors.lastname: Eilam
authors.forename: J
authors.initials: J
authors.lastname: Lotem
authors.forename: U
authors.initials: U
authors.lastname: Gat
authors.forename: F
authors.initials: F
authors.lastname: Otto
authors.forename: N
authors.initials: N
authors.lastname: Speck
authors.forename: Y
authors.initials: Y
authors.lastname: Groner
mesh_terms: D000818:Animals; D001846:Bone Development; D001842:Bone and Bones / Q000378:metabolism; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D006413:Hematopoietic System / Q000196:embryology; D007150:Immunohistochemistry; D051379:Mice; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; C493726:Runx1 protein, mouse; D014157:Transcription Factors