title: RP-HPLC method with electrochemical detection for the determination of metoclopramide in serum and its use in pharmacokinetic studies.
abstract: A rapid and sensitive reversed-phase high performance liquid chromatographic method has been developed for the determination of metoclopramide in serum. The assay was performed after single extraction with ethyl ether using methyl parahydroxybenzoate as internal standard. Chromatographic separations were performed on C(18) stationary phase with a mobile phase composed of methanol-phosphate buffer pH 3 (30:70 v/v). Analytes were detected electrochemically. The quantification limit for metoclopramide in serum was 2 ng mL(-1). Linearity of the method was confirmed in the range of 5-120 ng mL(-1) (correlation coefficient 0.9998). Within-day relative standard deviations (RSDs) ranged from 0.3 to 5.5% and between-day RSDs from 0.8 to 6.0%. The analytical method was successfully applied for the determination of pharmacokinetic parameters after ingestion of 10 mg dose of metoclopramide. Studies were performed on 18 healthy volunteers of both sexes.
authors.affiliation: Medical University of Gdansk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdansk, Poland. lamparcz@eniac.farmacja.amg.gda.pl
authors.forename: H
authors.initials: H
authors.lastname: Lamparczyk
authors.forename: A
authors.initials: A
authors.lastname: Chmielewska
authors.forename: L
authors.initials: L
authors.lastname: Konieczna
authors.forename: A
authors.initials: A
authors.lastname: Plenis
authors.forename: P K
authors.initials: PK
authors.lastname: Zarzycki
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000932:Antiemetics / Q000097:blood; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018492:Dopamine Antagonists / Q000097:blood; D004563:Electrochemistry; D005260:Female; D006801:Humans; D008297:Male; D008787:Metoclopramide / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results
chemical_list: D000932:Antiemetics; D018492:Dopamine Antagonists; D008787:Metoclopramide