Sulfogalactosylglycerolipid is involved in human gamete interaction.
Recent results from our laboratory have revealed the role of sulfogalactosylglycerolipid (SGG) in mouse sperm-zona pellucida (ZP) binding. In this report, we demonstrated the presence of SGG in Percoll-gradient centrifuged (PGC) human sperm by high performance thin layer chromatography with orcinol and Azure A staining, specific for glycolipids and sulfolipids, respectively. SGG in human PGC sperm was quantified by its affinity to Azure A to be 12-15 mol% of sperm lipids. Indirect immunofluorescence revealed that SGG existed on both live and aldehyde fixed human sperm in the head region. Pretreatment of human PGC sperm with affinity purified antiSGG Fab markedly inhibited sperm binding to the ZP in a concentration dependent manner, without any changes in the spontaneous acrosome rate or sperm motility parameters. Fluorescently labeled SGG liposomes also bound uniformly to isolated human ZP, while fluorescently labeled galactosylglycerolipid (GG, SGG's parental lipid) or phosphatidylserine (PS, negatively charged like SGG) liposomes did not. All of these results suggested the role of human sperm SGG in ZP binding.
Weerachatyanukul
W
W
Hormones/Growth/Development, Ottawa Health Research Institute, University of Ottawa Hospital, Ottawa, Ontario, Canada, K1Y 4E9.
Rattanachaiyanont
M
M
Carmona
E
E
Furimsky
A
A
Mai
A
A
Shoushtarian
A
A
Sirichotiyakul
S
S
Ballakier
H
H
Leader
A
A
Tanphaichitr
N
N
D020101:Acrosome Reaction / Q000187:drug effects; D001665:Binding Sites; D000001:Calcimycin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000032:analysis; D002855:Chromatography, Thin Layer; D005260:Female; D038983:Galactolipids; D006017:Glycolipids / Q000378:metabolism*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000494:pharmacology; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D010743:Phospholipids / Q000032:analysis; D013077:Sperm Head / Q000737:chemistry / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D015044:Zona Pellucida / Q000737:chemistry / Q000378:metabolism*
D038983:Galactolipids; D006017:Glycolipids; D007140:Immunoglobulin Fab Fragments; D008081:Liposomes; D010743:Phospholipids; C414346:sulfogalactosylglycerolipid; D000001:Calcimycin; D002784:Cholesterol