Molecular cloning, expression and characterization of the Canis familiaris interleukin-4.
Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis.
Wondimu
A
A
Institute of Immunology and Molecular Biology, Free University of Berlin, Philippstrasse 13, D-10115 Berlin, Germany.
Veit
M
M
Kohn
B
B
Kaul
S
S
Hoffmann
A
A
Brunnberg
L
L
Schmidt
M F
MF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001681:Biological Assay; D019556:COS Cells; D003001:Cloning, Molecular; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D014162:Transfection
D000911:Antibodies, Monoclonal; D015847:Interleukin-4