Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains.
The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use.
Pérez
M A
MA
Instituto Madrileño de Investigación Agraria y Alimentaria, Comunidad de Madrid, Finca El Encín, Madrid, Spain.
Gallego
F J
FJ
Hidalgo
P
P
D005821:Genetic Techniques; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics; D013045:Species Specificity