title: Spermatogenesis in the golden hamster: the role of c-kit.
abstract: c-kit is related to the family of transmembrane tyrosine kinase receptors. Mutations in genes for either c-kit or its ligand, Steel factor, result in infertility, but the role of c-kit/SCF system in spermatogenesis is not well understood. In this study Western blot analysis together with confocal microscopy were used to follow c-kit expression in hamsters during the first spermatogenic wave in mature animals and in old age. Three antibodies raised against different domains of c-kit were tested on Western Blot. Confocal microscopy was performed after incubation of fixed seminiferous tubules with tested antibodies followed by binding of FITC-labeled secondary antibody. Longitudinal sections of seminiferous tubule were observed by confocal microscopy to determine in which stages of spermatogenesis and in which cell types c-kit was found. C-kit bands of 80,140, and 150 kDa were observed on Western blot, indicating that c-kit is a name related to several proteins sharing some common domains. Only the band of 150 kDa correlated with positive staining of c-kit in tubules using confocal microscopy. We term this protein c-kit150T (150 kDa, testis). We demonstrated that c-kit150T appeared in differentiating hamster spermatogonia at stages VII-VIII of adult spermatogenesis and at day 13-14 during the first spermatogenic wave. It remained attached to the cell until late pachytene. This suggests that c-kit may play a role in preparing the germinal cells to enter meiosis. In order to evaluate the effect of aging on the number of germ cells, B2 spermatogonia/Sertoli cell ratio was calculated in the group of young animals (5-7 months) compared to this ratio in older ones (20-26 months). A significant decrease (P < 0.01) in the number of B2 spermatogonia in the group of old hamsters as compared to young ones was seen. The calculated value for the B2 spermatogonia/Sertoli cell ratio was 5.6 +/- 0.7 in young animals and 3.8 +/- 1.2 in the 20-26 months ones. In addition, decrease in the intensity of staining for c-kit was detected in the old hamsters. These may be the reasons for subfertility in old age and in other cases of testicular disorders.
authors.affiliation: Department of Clinical Biochemistry, Sackler Medical School, Tel Aviv University, Ramat Aviv, Israel. margarit@post.tau.ac.il
authors.forename: M
authors.initials: M
authors.lastname: Vigodner
authors.forename: L M
authors.initials: LM
authors.lastname: Lewin
authors.forename: L
authors.initials: L
authors.lastname: Shochat
authors.forename: R
authors.initials: R
authors.lastname: Golan
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D018613:Microscopy, Confocal; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D012671:Seminiferous Tubules / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D013094:Spermatozoa / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D019009:Proto-Oncogene Proteins c-kit