Immunohistochemical study of apoptosis of lens epithelial cells in human and diabetic rat cataracts.
OBJECTIVE
To evaluate apoptosis of lens epithelial cells by immunohistochemical methods.


METHODS
We performed terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assays on capsulotomy specimens (53 patients, 68 eyes) from patients who had undergone cataract surgery, and on the epithelium of diabetic cataracts in rats (72 rats, 144 eyes). An animal model of diabetic cataracts was prepared by injection of streptozotocin in 3-week-old rats. The specimens of rats were also examined using the proliferating cell nuclear antigen (PCNA) immunohistochemical staining method.


RESULTS
Although some TUNEL-positive cells were detected in capsulotomy specimens, we recognized little correlation between their distribution and the morphological classification of the cataracts. In the animal model of diabetic cataracts, TUNEL-positive cells were seen around the regions of accumulated epithelial cells. In the accumulated regions, PCNA-labeled cells undergoing DNA synthesis were also detected.


CONCLUSIONS
These results suggest the possibility that apoptosis occurs in human lens epithelial cells. Apoptosis and proliferation of lens epithelial cells may be induced by factors such as hyperglycemia.
Takamura
Y
Y
Department of Ophthalmology, Fukui Medical University, Yoshida-gun, Fukui-ken, Japan.
Sugimoto
Y
Y
Kubo
E
E
Takahashi
Y
Y
Akagi
Y
Y
D000368:Aged; D000818:Animals; D017209:Apoptosis; D002386:Cataract / Q000378:metabolism / Q000473:pathology*; D002387:Cataract Extraction; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D007903:Lens Capsule, Crystalline / Q000378:metabolism / Q000473:pathology; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D018809:Proliferating Cell Nuclear Antigen; D004247:DNA