title: Responses of anthocyanin-producing and non-producing cells of Glehnia littoralis to radical generators.
abstract: The responses of anthocyanin-producing (violet) and non-producing (white) cells of Glehnia littoralis to radical generators were compared. Cell growth, anthocyanin content, phenylalanine ammonia-lyase (PAL) activity and furanocoumarin production were determined after treatment with H(2)O(2), 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), X-ray and yeast extract, independently. AAPH and H(2)O(2) repressed the growth of both violet and white cells, but violet cells grew better than white cells. On the other hand, the anthocyanin content in violet cells decreased. Neither X-ray nor yeast extract affected cell growth or pigment production. Treatment with H(2)O(2), yeast extract, and X-ray, but not AAPH, induced PAL activity and furanocoumarin production in white cell cultures, whereas violet cell cultures did not produce furanocoumarin following any of the treatment employed.
authors.affiliation: School of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi 1-14, 852-8521, Nagasaki, Japan. k-yoshie@net.nagasaki-u.ac.jp
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Mina
authors.initials: M
authors.lastname: Ohta
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ikenaga
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000578:Amidines / Q000494:pharmacology*; D000872:Anthocyanins / Q000378:metabolism* / Q000528:radiation effects; D019661:Apiaceae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D003374:Coumarins / Q000819:agonists / Q000378:metabolism / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010650:Phenylalanine Ammonia-Lyase / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014965:X-Rays
chemical_list: D000578:Amidines; D000872:Anthocyanins; D003374:Coumarins; C046728:2,2'-azobis(2-amidinopropane); C030123:coumarin; D006861:Hydrogen Peroxide; D010650:Phenylalanine Ammonia-Lyase