title: The high-molecular-weight cytochrome c Cyc2 of Acidithiobacillus ferrooxidans is an outer membrane protein.
abstract: A high-molecular-weight c-type cytochrome, Cyc2, and a putative 22-kDa c-type cytochrome were detected in the membrane fraction released during spheroplast formation from Acidithiobacillus ferrooxidans. This fraction was enriched in outer membrane components and devoid of cytoplasmic membrane markers. The genetics, as well as the subcellular localization of Cyc2 at the outer membrane level, therefore make it a prime candidate for the initial electron acceptor in the respiratory pathway between ferrous iron and oxygen.
authors.affiliation: Laboratoire de Chimie Bactérienne, CNRS, IBSM, Marseille, France.
authors.forename: Andrés
authors.initials: A
authors.lastname: Yarzábal
authors.forename: Gaël
authors.initials: G
authors.lastname: Brasseur
authors.forename: Jeanine
authors.initials: J
authors.lastname: Ratouchniak
authors.forename: Karen
authors.initials: K
authors.lastname: Lund
authors.forename: Danielle
authors.initials: D
authors.lastname: Lemesle-Meunier
authors.forename: John A
authors.initials: JA
authors.lastname: DeMoss
authors.forename: Violaine
authors.initials: V
authors.lastname: Bonnefoy
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D003574:Cytochrome c Group / Q000302:isolation & purification*; D004579:Electron Transport; D020563:Gammaproteobacteria / Q000737:chemistry*; D010101:Oxygen Consumption; D017433:Protein Structure, Secondary; D013855:Thiobacillus / Q000737:chemistry*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003574:Cytochrome c Group