title: Mechanisms of cholinergic dysfunction in rabbits following recurrent aspiration of cow's milk.
abstract: Recurrent aspiration of cow's milk has been shown to alter neural control of airways in young rabbits (Gelfand et al., 1997). The purpose of this study was to define the mechanisms responsible for in vitro cholinergic hyperresponsiveness in this model. Beginning at 1 week of age, rabbits received either 0.5 mL/kg whole cow's milk or sterile saline intranasally while under light anesthesia. This was repeated each weekday for 2 weeks. At 8 weeks of age, rabbits were sacrificed. Portions of lungs underwent lavage with sterile saline. Tracheal smooth muscle (TSM) segments were also removed. Segments were assessed for acetylcholine (ACh) release by high-performance liquid chromatography ( HPLC) with electrochemical detection or acetylcholinesterase (AChE) kinetic activity by spectrophotometry. Substance P (SP), a neuropeptide that can increase ACh release from nerves, was also assessed using an enzyme immunoassay to define the content in lavage and TSM segments. Immunohistochemistry for SP within airways was also assessed. We found that recurrent aspiration of milk led to statistically significant alterations in many parameters. Acetylcholine release was significantly greater in segments of airways from rabbits that had aspirated cow's milk (27.5 +/- 1.7 vs. 20.1 +/- 1.6 pmol/min/g tissue) than saline. At the same time, AChE activity was less in the group that aspirated milk (8.7 +/- 0.4 vs. 10.2 +/- 0.5 nmol/min/mg protein) compared to saline. The amount of SP within both lavage as well as tissue homogenates was greater in the group that had aspirated the foreign protein (159.1 +/- 28.9 vs. 41.9 +/- 5.2 pmol/mg protein in lavage; 158.7 +/- 31.9 vs. 80.5 +/- 7.8 pmol/mg protein in tissues) than saline controls. While total cholinergic nerve density as assessed by choline acetyltransferase was not significantly different between groups, SP-positive immunoreactive nerves were easily identified in the group that aspirated cow's milk. This study suggests that cholinergic hyperresponsiveness caused by repeated aspiration of milk is due to several abnormalities, including prejunctional (increase in ACh release) as well as junctional (decrease in AChE) mechanisms within the airways. In addition, an upregulation of SP within airways is part of this process.
authors.affiliation: Division of Pediatric Pulmonary Medicine, Department of Pediatrics, National Jewish Medical and Research Center, University of Colorado School of Medicine, Denver, Colorado 80206, USA. larseng@njc.org
authors.forename: G L
authors.initials: GL
authors.lastname: Larsen
authors.forename: J
authors.initials: J
authors.lastname: Loader
authors.forename: D D
authors.initials: DD
authors.lastname: Nguyen
authors.forename: G N
authors.initials: GN
authors.lastname: Colasurdo
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004558:Electric Stimulation; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008892:Milk; D009119:Muscle Contraction / Q000502:physiology; D009130:Muscle, Smooth / Q000503:physiopathology*; D011015:Pneumonia, Aspiration / Q000503:physiopathology*; D011817:Rabbits; D012008:Recurrence; D015656:Respiratory Mechanics; D013373:Substance P / Q000032:analysis; D014132:Trachea / Q000294:innervation*
chemical_list: D013373:Substance P; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine