title: Contrast media increase vascular endothelial permeability by inhibiting nitric-oxide production.
abstract: OBJECTIVE
  Contrast media induce adverse effects including edema of the face, glottis, or lung. The endothelial function is maintained by nitric oxide (NO). The present study was designed to elucidate the role of NO in mediating endothelium-related adverse effects of contrast media.
  
  
  METHODS
  Human microvascular endothelial cells grown on a Transwell membrane were incubated with iohexol or ioxaglate in the absence or presence of N(G)-monomethyl-L-arginine or sodium nitroprusside. After washing cells, the permeability of sodium fluorescein or Evans blue albumin and the accumulation of NO(2)(-) was examined.
  
  
  RESULTS
  Contrast media (50-150 mgI/mL) dose-dependently increased the permeability coefficient by 30% to 230% and inhibited the formation of NO(2)(-) by 40% to 80%. Sodium nitroprusside and N(G)-monomethyl-L-arginine produced protective and aggravating effects on contrast media-increased permeability, respectively.
  
  
  CONCLUSIONS
  The present study suggested that contrast media increase vascular endothelial permeability by inhibiting NO production, leading to vascular endothelium-related adverse effects of contrast media.
authors.affiliation: Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Kyushu, Japan.
authors.forename: Wakako
authors.initials: W
authors.lastname: Furuta
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamauchi
authors.forename: Shinya
authors.initials: S
authors.lastname: Dohgu
authors.forename: Sinsuke
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Sendo
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Makino
authors.forename: Ryozo
authors.initials: R
authors.lastname: Oishi
authors.forename: Yasufumi
authors.initials: Y
authors.lastname: Kataoka
mesh_terms: D000704:Analysis of Variance; D002199:Capillary Permeability; D002478:Cells, Cultured; D003287:Contrast Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D019323:omega-N-Methylarginine / Q000494:pharmacology
chemical_list: D003287:Contrast Media; D004791:Enzyme Inhibitors; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D019323:omega-N-Methylarginine; D009569:Nitric Oxide