title: A new and simple procedure for the evaluation of the association of surfactants to proteins.
abstract: A new and simple method useful for the evaluation of the association of surfactants to proteins is proposed. The method is based on an analysis of the effect promoted by surfactant addition upon the fluorescence intensity of the intrinsic tryptophan chromophore and its dependence with protein concentration. The proposed methodology is applied to quantify the binding of an anionic (sodium dodecylsulfate), a zwitterionic (N-hexadecyl-N,N'-dimethyl-3-ammonio-1-propane-sulfonate) and a neutral (Triton X-100, reduced) surfactant to bovine serum albumin (BSA).
authors.affiliation: Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40-Correo 33, Santiago, Chile. elissi@lauca.usach.cl
authors.forename: Eduardo
authors.initials: E
authors.lastname: Lissi
authors.forename: Elsa
authors.initials: E
authors.lastname: Abuin
authors.forename: María E
authors.initials: ME
authors.lastname: Lanio
authors.forename: Carlos
authors.initials: C
authors.lastname: Alvarez
mesh_terms: D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D017830:Octoxynol / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry
chemical_list: C094641:N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D014364:Tryptophan; D017830:Octoxynol