Matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP in peritoneal fluids and sera and correlation with peritoneal adhesions.
OBJECTIVE
To assess the presence of matrix metalloproteinase (MMP-1) and tissue inhibitor of MMP (TIMP-1) in peritoneal fluid and serum of subjects with and without adhesions.


METHODS
Cross-sectional study.


METHODS
Academic research centers.


METHODS
Sixty-three patients who underwent abdominal/pelvic surgery.


METHODS
MMP-1, TIMP-1, and MMP-1-TIMP-1 complex content.


METHODS
ELISA.


RESULTS
Peritoneal fluids (PF) and sera of subjects with and without peritoneal adhesions contain MMP-1, TIMP-1, and MMP-1-TIMP-1 complex at varying levels with 10- to 100-fold higher TIMP-1 than MMP-1. Compared with serum, PF contains a lower level of MMP-1 in subjects with mild adhesions and without adhesions, higher TIMP-1 in subjects with extensive adhesions, and lower MMP-1-TIMP-1 complex in subjects with moderate adhesions. However, the serum and PF content of MMP-1, TIMP-1, and MMP-1-TIMP-1 complex was not statistically different among subjects with or without adhesions, with the exception of TIMP-1 in PF of subjects with extensive adhesions. MMP1-TIMP-1 ratio indicates that a major portion of MMP-1 is in complex with TIMP-1. There was no age- or gender-dependent difference in MMP-1 and TIMP-1 content in serum or PF.


CONCLUSIONS
Despite differences in MMP-1 and TIMP-1 levels in serum and PF of subjects with extensive and moderate adhesions, there is no correlation between MMP-1 and TIMP-1, with the exception of higher TIMP-1 in PF of subjects with extensive adhesions.
Chegini
N
N
Institute for Wound Research, Department of Obstetrics and Gynecology, University of Florida, Gainesville, Florida 32610-0294, USA. cheginin@obgyn.ufl.edu
Kotseos
K
K
Bennett
B
B
Diamond
M P
MP
Holmdahl
L
L
Burns
J
J
D000005:Abdomen / Q000601:surgery; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000378:metabolism* / Q000473:pathology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D010532:Peritoneal Diseases / Q000201:enzymology*; D011183:Postoperative Complications / Q000201:enzymology; D018709:Statistics, Nonparametric; D000267:Tissue Adhesions / Q000201:enzymology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism*; D014945:Wound Healing / Q000502:physiology
D019715:Tissue Inhibitor of Metalloproteinase-1; D020781:Matrix Metalloproteinase 1