title: CB1-cannabinoid and mu-opioid receptor co-localization on postsynaptic target in the rat dorsal horn.
abstract: Cannabinoids and opioids interact in the control of nociception at the spinal level. Likely, several mechanisms are involved, with one of them being co-localization of cannabinoid and opioid receptors. In order to validate this hypothesis, a double labeling study of CB1 cannabinoid receptors and mu-opioid receptors in the dorsal horn of the rat spinal cord was performed. A strong co-localization of CB1 and mu-opioid receptors was observed in lamina II interneurons at the ultrastructural level. The physiological consequences of the co-localization are discussed.
authors.affiliation: CNRS UMR 7624, Neurobiologie des Signaux Intercellulaires, Université Pierre et Marie Curie, 7 Quai Saint Bernard, 75252 Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Salio
authors.forename: J
authors.initials: J
authors.lastname: Fischer
authors.forename: M F
authors.initials: MF
authors.lastname: Franzoni
authors.forename: K
authors.initials: K
authors.lastname: Mackie
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Conrath
mesh_terms: D000818:Animals; D002186:Cannabinoids / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009619:Nociceptors / Q000378:metabolism* / Q000648:ultrastructure; D018847:Opioid Peptides / Q000378:metabolism; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D020671:Posterior Horn Cells / Q000378:metabolism* / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D002186:Cannabinoids; D018847:Opioid Peptides; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D017450:Receptors, Opioid, mu