The glycan structure of albumin Redhill, a glycosylated variant of human serum albumin.
Although human serum albumin is synthesized without carbohydrate, glycosylated variants of the protein can be found. We have determined the structure of the glycan bound to the double-mutant albumin Redhill (-1 Arg, 320 Ala-->Thr). The oligosaccharide was released from the protein using anhydrous hydrazine, and its structure was investigated using neuraminidase and a reagent array analysis method, which is based on the use of specific exoglycosidases. The glycan was shown to be a disialylated biantennary complex type oligosaccharide N-linked to 318 Asn. However, a minor part (11 mol%) of the glycan was without sialic acid. The structure is principally the same as that of glycans bound to two other types of glycosylated albumin variants. Glycosylation can affect, for example, the fatty acid binding properties of albumin. Taking the present information into account, it is apparent that different effects on binding are caused not by different glycan structures but by different locations of attachment, with the possible addition of local conformational changes in the protein molecule.
Kragh-Hansen
U
U
Department of Medical Biochemistry, The University of Aarhus, Denmark. ukh@biokemi.au.dk
Donaldson
D
D
Jensen
P H
PH
D002240:Carbohydrate Sequence; D003488:Cyanogen Bromide; D006026:Glycoside Hydrolases; D006031:Glycosylation; D006579:Heterozygote; D006801:Humans; D006834:Hydrazines; D008024:Ligands; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009439:Neuraminidase; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry* / Q000235:genetics; D000075462:Serum Albumin, Human
D006834:Hydrazines; D008024:Ligands; D009844:Oligosaccharides; D010446:Peptide Fragments; D011134:Polysaccharides; D012709:Serum Albumin; C047510:albumin Redhill; C029424:hydrazine; D006026:Glycoside Hydrolases; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D003488:Cyanogen Bromide; D000075462:Serum Albumin, Human