Effect of tenascin-X together with vascular endothelial growth factor A on cell proliferation in cultured embryonic hearts.
Tenascin-X (TNX) is a large glycoprotein that appears in extracellular matrices. Previously, we demonstrated that TNX binds to vascular endothelial growth factors A and B (VEGF-A and -B) and that VEGF-B in combination with TNX induces DNA synthesis in endothelial cells via increased signals mediated by the VEGFR-1 receptor. In this study, we investigated the effect of TNX with VEGF-A on the cell proliferation in embryonic mouse heart explants from either wild-type (TNX+/+) or TNX-deficient (TNX-/-) mice. The addition of VEGF-A to the explants from TNX+/+ mice increased cell proliferation by 1.5 fold compared with that in TNX-/- mice, indicating that TNX with VEGF family member plays an important role in the control of endothelial cell proliferation in vivo.
Ikuta
T
T
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Ariga
H
H
Matsumoto
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KI
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D004622:Embryo, Mammalian; D004699:Endocardium / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D011247:Pregnancy; D019063:Tenascin / Q000172:deficiency / Q000235:genetics / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A; D042561:Vascular Endothelial Growth Factor B
D016228:Endothelial Growth Factors; D019063:Tenascin; D042461:Vascular Endothelial Growth Factor A; D042561:Vascular Endothelial Growth Factor B; C081865:tenascin X