Application of manual and automated systems for purification of ochratoxin a and zearalenone in cereals with immunoaffinity columns.
A manual vacuum manifold and an automated solid phase extraction (ASPEC) system were applied for purification of ochratoxin A and zearalenone in wheat, rye, barley, and oat samples with immunoaffinity columns followed by separation with a high-performance liquid chromatograph and fluorescence detection. The immunoaffinity columns for manual sample purification were purchased from a different manufacturer than were those for the automated system. The limit of detection (LOD) for the method for ochratoxin A with a vacuum manifold and ASPEC was 0.1 microg/kg. For the method for zearalenone, the LODs were 1.5 microg/kg with a vacuum manifold and 3 microg/kg with ASPEC. For the methods for ochratoxin A at spiking levels of 0.6 and 2.5 microg/kg, mean recoveries for different cereals varied from 68 to 106%. For the methods for zearalenone, mean recoveries varied from 78 to 117% at spiking levels of 9 and 25 microg/kg. The relative standard deviations of repeatability with various cereals employing both methods were 2-15 and 2-19% for ochratoxin A and zearalenone, respectively.
Eskola
Mari
M
National Veterinary and Food Research Institute (EELA), Department of Chemistry, P.O. Box 45 (Hämeentie 57), FIN-00581 Helsinki, Finland. mari.eskola@eela.fi
Kokkonen
Meri
M
Rizzo
Aldo
A
D002273:Carcinogens / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D004968:Estrogens, Non-Steroidal / Q000302:isolation & purification; D005453:Fluorescence; D009793:Ochratoxins / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015025:Zearalenone / Q000302:isolation & purification*
D002273:Carcinogens; D004968:Estrogens, Non-Steroidal; D009793:Ochratoxins; C025589:ochratoxin A; D015025:Zearalenone