title: Electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum and chromosomal location of several xylanolytic genes.
abstract: The electrophoretic karyotype of the filamentous fungus Penicillium purpurogenum has been resolved. Using contour-clamped homogeneous electric field gel electrophoresis, five chromosomal bands were separated, with estimated sizes of 7.1, 5.2, 3.7, 2.9 and 2.3 Mbp, giving a total genome size of 21.2 Mbp. To our knowledge, this is the smallest Penicillium genome determined so far. By Southern blots and using homologous probes, the chromosomal location of five xylanolytic genes from P. purpurogenum was determined: axeI (acetyl xylan esterase I), xynB (endoxylanase B) and abf1 (arabinofuranosidase 1) in chromosome I, xynA (endoxylanase A) in chromosome II, and axeII (acetyl xylan esterase II) in chromosome III. This is the first study where the location of xylanase genes in a Penicillium genome has been established.
authors.affiliation: Departamento de Genética Molecular y Microbiología, Pontifica Universidad Católica de Chile, Santiago.
authors.forename: R
authors.initials: R
authors.lastname: Chávez
authors.forename: F
authors.initials: F
authors.lastname: Fierro
authors.forename: F
authors.initials: F
authors.lastname: Gordillo
authors.forename: J
authors.initials: J
authors.lastname: Francisco Martín
authors.forename: J
authors.initials: J
authors.lastname: Eyzaguirre
mesh_terms: D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D016521:Electrophoresis, Gel, Pulsed-Field; D005800:Genes, Fungal; D007621:Karyotyping / Q000379:methods; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000235:genetics*
chemical_list: D014990:Xylans; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase