In vitro characterization of Mato's FGP cells isolated from rat cerebrum.
Mato's FGP cells surrounding cerebral arterioles play a significant role in the maintenance of a homeostatic microenvironment in the brain. In this study, the perivascular cells were isolated from rat cerebral microvessels and cultured in vitro to characterize their phenotype. Autofluorescence of the intracellular granules in cultured cells and the uptake of HRP and DiI-Ac-LDL by these cells were observed. The cells reacted positively to an anti-scavenger receptor A antibody. Positive immunoreactions of cultured cells to ED1 and ED2 antibodies were observed, whereas they were weak or negative to ED3 and OX42 antibodies. Acid phosphatase activity was detected in the granules of cultured cells. In conclusion, the cells cultivated under the present conditions revealed very similar characteristics to Mato's FGP cells in situ and therefore are useful for studies on FGP cells.
Nakazawa
Tomoko
T
Department of Anatomy and Developmental Biology, Medical School, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. tnaka@research.twmu.ac.jp
Nishikawa
Megumi
M
Aikawa
Eizo
E
Mato
Masao
M
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D001160:Arterioles / Q000378:metabolism* / Q000648:ultrastructure; D030161:Avian Proteins; D051926:Basigin; D001798:Blood Proteins; D018955:CD36 Antigens / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008854:Microscopy, Electron; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D051117:Scavenger Receptors, Class A
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D018955:CD36 Antigens; D008562:Membrane Glycoproteins; D051117:Scavenger Receptors, Class A; D051926:Basigin; D000135:Acid Phosphatase