Agonists of proteinase-activated receptor 2 excite guinea pig ileal myenteric neurons.
The effects of proteinase-activated receptor 2 (PAR2) agonists on the electrical properties of intact guinea pig ileal myenteric neurons were measured with intracellular microelectrodes. Approximately 52% of AH neurons and 41% of S neurons responded to pressure ejection of SLIGRL-NH(2) or trypsin with a prolonged depolarization that was often accompanied by increased excitability. When added to the bathing solution, trypsin caused a concentration-dependent depolarization of responding neurons with an estimated EC(50) value of 87 nM. Collectively, these novel observations indicate that PAR2 excites a proportion of myenteric neurons, which may contribute to dysmotility during intestinal inflammation.
Linden
D R
DR
Given C 423, Department of Anatomy and Neurobiology, The University of Vermont College of Medicine, Burlington, VT 05405, USA.
Manning
B P
BP
Bunnett
N W
NW
Mawe
G M
GM
D000818:Animals; D005769:Gastrointestinal Motility; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects* / Q000294:innervation; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008839:Microelectrodes; D009197:Myenteric Plexus / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology; D044464:Receptor, PAR-2; D018179:Receptors, Thrombin / Q000819:agonists*; D014357:Trypsin / Q000494:pharmacology
D009842:Oligopeptides; D044464:Receptor, PAR-2; D018179:Receptors, Thrombin; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D014357:Trypsin