Expression levels of transdominant peptides and proteins in Saccharomyces cerevisiae.
From libraries of peptides and protein fragments, several inhibitors that block pheromone response in Saccharomyces cerevisiae have been isolated previously. In many cases, the inhibitors are displayed as part of a scaffold, such as green fluorescent protein. Each of the inhibitors has a characteristic physiological strength or genetic penetrance. In this report, the roles of expression level and display scaffold on the activities of a subset of pheromone-response pathway inhibitors were examined. Special consideration was given to the relationship between expression levels of specific inhibitors, which may exceed 50 microM in some instances, and penetrance.
Sandrock
Tanya
T
Deltagen Proteomics, Inc., 615 Arapeen Drive, Suite 300, Salt Lake City, UT 84108, USA. Tsandrock@arcanis.com
Poritz
Mark
M
Kim
Marianne
M
Feldhaus
Michael J
MJ
Roth
Bruce
B
Caponigro
Giordano
G
Kamb
Alexander
A
D033942:Adaptor Proteins, Vesicular Transport; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D010675:Pheromones / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D016335:Zinc Fingers
D033942:Adaptor Proteins, Vesicular Transport; D005656:Fungal Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; C071657:PEP3 protein, S cerevisiae; D010455:Peptides; D010675:Pheromones; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins