Thrombolysis effect with FIIa from Agkistrodon acutus venom in different thrombosis model.
OBJECTIVE
A fibrinolytic enzyme from Agkistrodon acutus venom, called FIIa, was tested for thrombolytic activity in animals.


METHODS
Carotid thrombosis model in rats and rabbits and middle cerebral artery (MCA) thrombosis model in rats were used.


RESULTS
Intravenous administration of FIIa, at a dosage of 0.625 mg/kg, resulted in thrombolysis of carotid thrombi. However, in middle cerebral artery thrombosis, the effective thrombolysis dose was 1.25 mg/kg. When the dosage of FIIa increased, the thrombolytic effect was stronger. Histological examination of kidney, liver, heart, and lung tissue showed no hemorrhage.


CONCLUSIONS
It shows that FIIa from Agkistrodon acutus venom is able to solubilize thrombus in vivo without hemorrhage at an effective dose for thrombolysis.
Chen
J H
JH
Department of Pharmacology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China.
Liang
X X
XX
Qiu
P X
PX
Yan
G M
GM
D017836:Agkistrodon; D000818:Animals; D002339:Carotid Arteries; D003435:Crotalid Venoms / Q000009:adverse effects / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D005343:Fibrinolytic Agents / Q000009:adverse effects / Q000627:therapeutic use*; D006470:Hemorrhage / Q000139:chemically induced; D008297:Male; D008666:Metalloendopeptidases; D020768:Middle Cerebral Artery; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000188:drug therapy*
D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; C478420:FIIa; D008666:Metalloendopeptidases