alpha-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome.
The Clostridium josui aga27A gene encodes the cellulosomal alpha-galactosidase Aga27A, which comprises a catalytic domain of family 27 of glycoside hydrolases and a dockerin domain responsible for cellulosome assembly. The catalytic domain is highly homologous to those of various alpha-galactosidases of family 27 of glycoside hydrolases from eukaryotic organisms, especially plants. The recombinant Aga27A alpha-galactosidase devoid of the dockerin domain preferred highly polymeric galactomannan as a substrate to small saccharides such as melibiose and raffinose.
Jindou
Sadanari
S
Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.
Karita
Shuichi
S
Fujino
Emi
E
Fujino
Tsuchiyoshi
T
Hayashi
Hidenori
H
Kimura
Tetsuya
T
Sakka
Kazuo
K
Ohmiya
Kunio
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002480:Cellulase; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008351:Mannans / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016296:Mutagenesis; D015388:Organelles / Q000201:enzymology; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D000519:alpha-Galactosidase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D004269:DNA, Bacterial; D008351:Mannans; C012990:galactomannan; D000519:alpha-Galactosidase; D002480:Cellulase; D005690:Galactose