title: Novel non-glycerol-based cytofectins with lactic acid-derived head groups.
abstract: We report herein the design, synthesis, and transfection biology of a novel series of non-glycerol-based cationic lipids with lactic acid-derived head groups The synthetic procedure adopted herein for preparing 1-hydroxy-prop-2-yl head-group-based monocationic transfection lipids 1-7 is fairly straightforward and potentially applicable in designing other cationic lipids with lactic acid-derived head groups. A striking anchor-length dependency was observed in NIH3T3 cells in the sense that except lipid 4, all the other lipids were essentially transfection-inefficient. Ethidium bromide assay for the lipid:DNA interactions is consistent with the general observation that significant lipid:DNA interactions do not guarantee on improved transfection efficiency cationic lipid mediated gene delivery. Given its remarkable transfection properties and low cellular toxicity, lipid 4 is likely to find future use in the area of liposomal gene delivery.
authors.affiliation: Division of Lipid Science and Technology, Indian Institute of Chemical Technology, Hyderabad, 500 007, India.
authors.forename: A A
authors.initials: AA
authors.lastname: Laxmi
authors.forename: P
authors.initials: P
authors.lastname: Vijayalakshmi
authors.forename: T N
authors.initials: TN
authors.lastname: Kaimal
authors.forename: A
authors.initials: A
authors.lastname: Chaudhuri
authors.forename: Y
authors.initials: Y
authors.lastname: Ramadas
authors.forename: N M
authors.initials: NM
authors.lastname: Rao
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019556:COS Cells; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems; D004996:Ethidium; D005456:Fluorescent Dyes; D015316:Genetic Therapy; D005990:Glycerol / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D008055:Lipids / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D014162:Transfection
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids; D008081:Liposomes; D019344:Lactic Acid; D004247:DNA; D004996:Ethidium; D005990:Glycerol