Effect of environmental and chemotactic stimuli on the activity of the Campylobacter jejuni flaA sigma(28) promoter.
The effect of environmental and chemotactic stimuli was assessed using a fusion between the Campylobacter jejuni flaA sigma(28) promoter and a promoterless luxCDABE cassette on pRYluxCDABE. Environmental stimuli relevant to the enteric environment were found to modulate flaA promoter activity. pH, bovine bile, deoxycholate, L-fucose and osmolarity up-regulated the flaA promoter while viscosity resulted in down-regulation. C. jejuni chemotactic effectors, including the amino acids aspartate and glutamate and the organic acids citrate, fumarate, alpha-ketoglutarate and succinate up-regulated the flaA promoter. Proline, which does not elicit a chemotactic response, up-regulated the flaA promoter.
Allen
K J
KJ
Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. kevin_allen@hc-sc.gc.ca
Griffiths
M W
MW
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001646:Bile; D016123:Campylobacter jejuni / Q000235:genetics / Q000502:physiology*; D002417:Cattle; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D003840:Deoxycholic Acid / Q000494:pharmacology; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D005643:Fucose / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; C064971:FliA protein, Bacteria; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D003840:Deoxycholic Acid; D005408:Flagellin; C067138:flaA protein, bacteria; D005643:Fucose