title: Detection of quantitative trait loci for resistance/susceptibility to pseudorabies virus in swine.
abstract: This study describes genetic differences in resistance/susceptibility to pseudorabies virus (PrV) between European Large White and Chinese Meishan pigs, with a mapping of quantitative trait loci (QTL) obtained from a genome-wide scan in F(2) animals. Eighty-nine F(2) pigs were challenged intranasally at 12 weeks with 10(5) p.f.u. of the wild-type PrV strain NIA-3. For QTL analysis, 85 microsatellite markers, evenly spaced on the 18 porcine autosomes and on the pseudoautosomal region of the X chromosome, were genotyped. All pigs developed clinical signs, i.e. fever, from 3 to 7 days p.i. The pure-bred Large White pigs, the F(1) and three-quarters of the F(2) animals, but none of the Meishan pigs, developed neurological symptoms and died or were euthanized. QTLs for appearance/non-appearance of neurological symptoms were found on chromosomes 9, 5, 6 and 13. They explained 10.6-17.9% of F(2) phenotypic variance. QTL effects for rectal temperature after PrV challenge were found on chromosomes 2, 4, 8, 10, 11 and 16. Effects on chromosomes 9, 10 and 11 were significant on a genome-wide level. The results present chromosomal regions that are associated with presence/absence of neurological symptoms as well as temperature course after intranasal challenge with NIA-3. The QTLs are in proximity to important candidate genes that are assumed to play crucial roles in host defence against PrV.
authors.affiliation: Department of Animal Breeding and Biotechnology, University of Hohenheim, Garbenstraße 17, D-70593 Stuttgart, Germany1.
authors.forename: Gerald
authors.initials: G
authors.lastname: Reiner
authors.affiliation: Department of Animal Breeding and Biotechnology, University of Hohenheim, Garbenstraße 17, D-70593 Stuttgart, Germany1.
authors.forename: Elke
authors.initials: E
authors.lastname: Melchinger
authors.affiliation: Department of Animal Breeding and Biotechnology, University of Hohenheim, Garbenstraße 17, D-70593 Stuttgart, Germany1.
authors.forename: Marcela
authors.initials: M
authors.lastname: Kramarova
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, D-72076 Tübingen, Germany2.
authors.forename: Eberhardt
authors.initials: E
authors.lastname: Pfaff
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, D-72076 Tübingen, Germany2.
authors.forename: Matthias
authors.initials: M
authors.lastname: Büttner
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, D-72076 Tübingen, Germany2.
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.affiliation: Department of Animal Breeding and Biotechnology, University of Hohenheim, Garbenstraße 17, D-70593 Stuttgart, Germany1.
authors.forename: Hermann
authors.initials: H
authors.lastname: Geldermann
mesh_terms: D000818:Animals; D020022:Genetic Predisposition to Disease / Q000235:genetics; D011558:Herpesvirus 1, Suid; D007113:Immunity, Innate / Q000235:genetics; D010641:Phenotype; D011557:Pseudorabies / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D013552:Swine; D014960:X Chromosome