title: Implication of co-measured platelet factor 4 in the reliability of the results of the plasma transforming growth factor-beta 1 measurement.
abstract: We examined the possible alteration of circulating transforming growth factor-beta1 (TGF-beta1) concentrations in a time-dependent fashion in human plasma. Plasma TGF-beta1 was measured three times at 2 week-intervals from each of 12 healthy participants. Platelet factor 4 (PF4) was measured in parallel with TGF-beta1 to estimate the degree of platelet degranulation. TGF-beta1 levels of the second and third plasma samples, in which PF4s were measured as < approximately 1000 IU/ml, were relatively low and fell in a narrow range. However, TGF-beta1 levels of the first samples, in most of which PF4s were > approximately 1000 IU/ml, appeared much higher and more variable than those of the second or third samples. These results indicate that the platelet degranulation accounted for the higher TGF-beta1 levels in the first samples, and thus did not support our initial assumption. We, nevertheless, could propose a useful guidance in the assessment of TGF-beta1 levels in plasma. When the PF4 level is measured as < approximately 1000 IU/ml under our assay conditions, the TGF-beta1 level in a given plasma sample might be accepted as a reliable value considering the effect of platelet degranulation on TGF-beta1 level.
authors.affiliation: Department of Neurobiology, Gyeongsang National University Graduate School, Chinju 660-702, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Jeon
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: E J
authors.initials: EJ
authors.lastname: Choi
authors.forename: S
authors.initials: S
authors.lastname: Cheon
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Jang
authors.forename: W S
authors.initials: WS
authors.lastname: Ha
authors.forename: S T
authors.initials: ST
authors.lastname: Park
authors.forename: C S
authors.initials: CS
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Park
authors.forename: B K
authors.initials: BK
authors.lastname: Park
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000473:pathology*; D002624:Chemistry, Clinical / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010978:Platelet Factor 4 / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000097:blood*; D053773:Transforming Growth Factor beta1
chemical_list: C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D010978:Platelet Factor 4