Influence of CETP gene variation on plasma lipid levels and coronary heart disease: a survey in Taiwan.
Cholesteryl ester transfer protein (CETP) transfers cholesteryl ester from high-density lipoprotein (HDL) to very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and chylomicron in exchange for triglycerides. Two CETP genetic variation and four polymorphisms are investigated by polymerase chain reaction (PCR) and restriction enzyme digestion in a population of Taiwan. The results show that a very rare variation frequency is found for CETP intron 14 splice site G-->A change. The population shows a predominant 405Ile allele (61%), 442Asp (97.7%), intron 1Taq1B(+) G allele (52%), intron 8 Msp1(-) A allele (89%) and intron 9 EcoN1(-) T allele (59.2%) in the control group. Patients with coronary heart disease (CHD) have more CETP EcoN1(+/+) GG genotype (25.3%) than the controls (13.6%) (P=0.049). The intron 1 Taq1B(-) A allele is associated with a high HDL cholesterol and apoA1 level, the EcoN1(+) G allele with a low apoA1 level and the 442Gly with both high total cholesterol and LDL cholesterol levels. Paradoxically, the 442Gly is also present with a higher frequency (5.2%) in HDL cholesterol > or =65 mg/dl group than that in the general population (2.3%) (P=0.04).
Wu
J H
JH
Department of Microbiology and Immunology, College of Medicine, Chang-Gung University, 259 Wen Hua I Road, Tao Yuan 333, Kwei San, Taiwan. jhwu@mail.cgu.edu.tw
Lee
Y T
YT
Hsu
H C
HC
Hsieh
L L
LL
D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D016022:Case-Control Studies; D053480:Cholesterol Ester Transfer Proteins; D003327:Coronary Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D006023:Glycoproteins; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000097:blood*; D008074:Lipoproteins / Q000032:analysis / Q000097:blood; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011159:Population Surveillance; D015995:Prevalence; D012016:Reference Values; D012307:Risk Factors; D012680:Sensitivity and Specificity; D017678:Sex Distribution; D013624:Taiwan / Q000453:epidemiology
C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D006023:Glycoproteins; D008055:Lipids; D008074:Lipoproteins