title: Induction of hepatitis C virus-specific cytotoxic T lymphocytes in mice by immunization with dendritic cells treated with an anthrax toxin fusion protein.
abstract: As a novel and safe vaccine strategy, the anthrax toxin-mediated antigen delivery system composed of lethal factor (LF) fusion protein and protective antigen (PA) has been studied to prime hepatitis C virus (HCV) core-specific cytotoxic T lymphocytes (CTLs) in vivo. The core epitope fused to LF (LF-core) together with PA induces a negligible core-specific CTL response in mice, whereas core-specific CTL are effectively primed in mice by injecting dendritic cells (DCs) treated in vitro with LF-core and PA. These findings imply that LF fusion protein plus PA in combination with dendritic cells may be useful for a novel T cell vaccine against HCV infection.
authors.affiliation: Department of Microbiology, Saitama Medical School, Moroyama-Cho, Iruma-Gun, Saitama 350-0495, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Moriya
authors.forename: M
authors.initials: M
authors.lastname: Matsui
authors.forename: M
authors.initials: M
authors.lastname: Osorio
authors.forename: H
authors.initials: H
authors.lastname: Miyazawa
authors.forename: C M
authors.initials: CM
authors.lastname: Rice
authors.forename: S M
authors.initials: SM
authors.lastname: Feinstone
authors.forename: S H
authors.initials: SH
authors.lastname: Leppla
authors.forename: J M
authors.initials: JM
authors.lastname: Keith
authors.forename: T
authors.initials: T
authors.lastname: Akatsuka
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D014761:Viral Hepatitis Vaccines; C030325:anthrax toxin; C069130:nucleocapsid protein, Hepatitis C virus