Measurement of small scalar and dipolar couplings in purine and pyrimidine bases.
A suite of spin-state-selective excitation (S3E) NMR experiments for the measurements of small one-bond (13C-13C, 15N-13C) and two-bond (1H-13C, 1H-15N) coupling constants in 13C,15N labeled purine and pyrimidine bases is presented. The incorporation of band-selective shaped pulses, elimination of the cross talk between alpha and beta sub-spectra, and accuracy and precision of the proposed approach are discussed. Merits of using S3E rather than alpha/beta-half-filter are demonstrated using results obtained on isotopically labeled DNA oligonucleotides.
Zídek
L
L
Masaryk University, Faculty of Science, National Centre for Biomolecular Research, Brno, Czech Republic.
Wu
H
H
Feigon
J
J
Sklenár
V
V
D001483:Base Sequence; D002247:Carbon Isotopes; D006859:Hydrogen; D015394:Molecular Structure; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D011687:Purines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides; D011687:Purines; D011743:Pyrimidines; D006859:Hydrogen