Phosphatidylglycerophosphate synthases from Arabidopsis thaliana.
Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N-terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C-terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP-diacylglycerol species specificity.
Müller
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F
RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringer Weg 1, 52056, Aachen, Germany.
Frentzen
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M
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D003567:Cytidine Diphosphate Diglycerides; D007527:Isoenzymes; D011994:Recombinant Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase