Expression of Tcf/Lef and sFrp and localization of beta-catenin in the developing mouse lung.
Recent evidence that Wnts and other genes in the Wnt signaling pathway are expressed in embryonic and adult mouse lung suggests that this pathway is important for cell fate decisions and differentiation of lung cell types. We therefore examined the expression and protein distribution of several Wnt pathway components during prenatal mouse lung development using whole-mount in situ hybridization and immunohistochemistry. Between embryonic days 10.5 and 17.5 (E10.5-E17.5), beta-catenin was localized in the cytoplasm, and often also the nucleus, of the undifferentiated primordial epithelium (PE), differentiating alveolar epithelium (AE; present from E14.5 onward), and adjacent mesenchyme. Tcf1, Lef1, Tcf3, Tcf4, sFrp1, sFrp2 and sFrp4 were also expressed in the PE, AE, and adjacent mesenchyme in specific spatio-temporal patterns.
Tebar
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M
Department of Pulmonology, Leiden University Medical Center, NL-2300 RC, Leiden, The Netherlands.
Destrée
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de Vree
W J
WJ
Ten Have-Opbroek
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D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D006023:Glycoproteins / Q000096:biosynthesis*; D051538:Hepatocyte Nuclear Factor 1-alpha; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008168:Lung / Q000196:embryology*; D051821:Lymphoid Enhancer-Binding Factor 1; D051379:Mice; D051822:T Cell Transcription Factor 1; D013997:Time Factors; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis*; D051176:beta Catenin; D000099274:Frizzled-Related Proteins
D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D051538:Hepatocyte Nuclear Factor 1-alpha; D047908:Intracellular Signaling Peptides and Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D051822:T Cell Transcription Factor 1; D015534:Trans-Activators; D014157:Transcription Factors; D051176:beta Catenin; D000099274:Frizzled-Related Proteins; C495267:CTNNB1 protein, mouse; C496488:Hnf1a protein, mouse; C497574:Lef1 protein, mouse