Flavanols and procyanidins of cocoa and chocolate inhibit growth and polyamine biosynthesis of human colonic cancer cells.
The effects of cocoa powder and extracts with different amounts of flavanols and related procyanidin oligomers were investigated on the growth of Caco-2 cells. Treatment of the cells with 50 microg/ml of procyanidin-enriched (PE) extracts caused a 70% growth inhibition with a blockade of the cell cycle at the G2/M phase. PE extracts caused a significant decrease of ornithine decarboxylase and S-adenosylmethionine decarboxylase activities, two key enzymes of polyamine biosynthesis. This led to a decrease in the intracellular pool of the polyamines. These observations indicate that polyamine metabolism might be an important target in the anti-proliferative effects of cocoa polyphenols.
Carnésecchi
Stéphanie
S
Laboratory of Nutritional Chemoprevention in Digestive Oncology, IRCAD, 1 place de l'hôpital, 67091, Strasbourg, France.
Schneider
Yann
Y
Lazarus
Sheryl A
SA
Coehlo
David
D
Gossé
Francine
F
Raul
Francis
F
D000970:Antineoplastic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D044946:Biflavonoids; D002099:Cacao / Q000737:chemistry*; D002392:Catechin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011073:Polyamines / Q000378:metabolism*; D044945:Proanthocyanidins; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D000975:Antioxidants; D044946:Biflavonoids; D005419:Flavonoids; D010936:Plant Extracts; D011073:Polyamines; D044945:Proanthocyanidins; C404987:flavan-3-ol; C017674:procyanidin; D002392:Catechin