title: Enhancement of X-ray-induced breaks in DNA bound to molecules containing platinum: a possible application to hadrontherapy.
abstract: Complexes made of DNA and chloroterpyridine platinum (PtTC) bound to plasmid DNA were placed in aqueous solution and irradiated with monochromatic X rays tuned to the resonant photoabsorption energy of the L(III) shell of the platinum atom. The number of single- and double-strand breaks (SSBs and DSBs) induced by irradiation on a supercoiled DNA plasmid was measured by the production of the circular-nicked and linear forms. To distinguish the contribution of the direct effects of ionization from the indirect effects due to a free radical attack, experiments were also performed in the presence of a hydroxyl free radical scavenger, dimethyl sulfoxide (DMSO). An enhancement of the number of SSBs and DSBs was observed when the plasmids contained the platinum intercalating molecules. A quantitative analysis was made to evaluate the respective contributions of the direct effects (Auger effect) and the indirect effects (free radical attack) to the number of DNA strand breaks. Even when off-resonant X rays were used, the strand break efficiency remained higher than expected based upon the absorption cross section, suggesting that the platinum bound to DNA might be increasing the yield of strand breaks. A mechanism is suggested that involves photoelectrons generated from the ionization of water which efficiently ionize platinum atoms. If this mechanism is correct, then heavy atoms, with a large cross section for ionization by electrons that are bound to the DNA, should behave as a radiosensitizer. This observation may provide insight into understanding the effects of new radiotherapy protocols, related chemotherapeutic agents such as cisplatin, and conventional radiotherapy for the treatment of tumors. A possible way to deliver the dose selectively in a well-defined volume, which uses the properties of the linear energy transfer of atomic ions interacting with matter, is suggested.
authors.affiliation: Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Oho, Tsukuba, Ibaraki 305-0801, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Frohlich
authors.forename: N
authors.initials: N
authors.lastname: Usami
authors.forename: K
authors.initials: K
authors.lastname: Takakura
authors.forename: C
authors.initials: C
authors.lastname: Le Sech
mesh_terms: D019457:Chromosome Breakage; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D018736:DNA Adducts / Q000528:radiation effects*; D004269:DNA, Bacterial / Q000737:chemistry / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000528:radiation effects; D004278:DNA, Superhelical / Q000737:chemistry / Q000528:radiation effects*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D016166:Free Radical Scavengers / Q000494:pharmacology; D018499:Linear Energy Transfer; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000528:radiation effects*; D010777:Photochemistry; D011837:Radiation-Protective Agents / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000737:chemistry*; D011878:Radiotherapy / Q000379:methods
chemical_list: D018736:DNA Adducts; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D016166:Free Radical Scavengers; D009944:Organoplatinum Compounds; D011837:Radiation-Protective Agents; D011838:Radiation-Sensitizing Agents; C021975:chloroterpyridineplatinum(II); D004247:DNA; D004121:Dimethyl Sulfoxide