Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene.
HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.
Chen
Guomin
G
Department of Tumor Virus and HIV, Institute of Virology, CAPM, Beijing, China.
Wang
Shuhui
S
Xiong
Kun
K
Wang
Jinzhong
J
Ye
Tao
T
Dong
Wenping
W
Wang
Qi
Q
Chen
Qimin
Q
Geng
Yunqi
Y
Wood
Charles
C
Zeng
Yi
Y
D016915:AIDS Vaccines; D000818:Animals; D002417:Cattle; D002460:Cell Line; D015750:Genes, gag / Q000235:genetics*; D015751:Genes, pol / Q000235:genetics*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D016915:AIDS Vaccines; D011993:Recombinant Fusion Proteins