Cloning of a cDNA encoding an E2 ubiquitin-conjugating enzyme from Catharanthus roseus: expression analysis in plant organs and in response to hormones in cell suspensions.
A novel cDNA (Crubie2) encoding ubiquitin-conjugating enzyme E2 was isolated from a Catharanthus roseus cDNA library. Sequence comparison with Arabidopsis thaliana E2 sequences revealed that CrUBIE2 is a member of a new plant E2 sub-family. Expression of Crubie2 is repressed in developing organs and down-regulated by cytokinin suggesting that a decrease in the ubiquitin-dependent proteolytic pathway may take part in the regulation of alkaloid biosynthesis in C. roseus cell suspensions.
Sibéril
Yann
Y
EA 2106, Biomolécules et Biotechnologies Végétales, Université de Tours, UFR des Sciences et Techniques, Laboratoire de Physiologie Végétale, Parc de Grandmont, 37200 Tours, France.
Thiersault
Martine
M
Nepumoceno
Gonçalo
G
Doireau
Pierre
P
Gantet
Pascal
P
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D030008:Catharanthus / Q000201:enzymology / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003583:Cytokinins / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008025:Ligases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D044763:Ubiquitin-Conjugating Enzymes
D003583:Cytokinins; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases