Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N') of Riemerella anatipestifer.
The generation of protective immunity against Riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion's proteins of OmpA, a 42kDa major outer membrane protein, and P45N', a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N' were isolated from R. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90), respectively. Immunoblotting and ELISA results showed that the purified recombinant proteins induced the production of antibodies in immunized ducks. However, neither was protective against subsequent challenge with the virulent serotype 15 strain, 34/90. All the five ducks immunized with formalinized R. anatipestifer strain 34/90 survived the challenge with the homologous strain whereas six out of seven ducks in the non-immunized control group died within a week following the challenge.
Huang
Bin
B
Institute of Molecular Agrobiology, National University of Singapore, Singapore.
Subramaniam
Sumathi
S
Frey
Joachim
J
Loh
Hilda
H
Tan
Hai-Meng
HM
Fernandez
Charlene J
CJ
Kwang
Jimmy
J
Chua
Kim-Lee
KL
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics; D016946:Gram-Negative Aerobic Rods and Cocci / Q000235:genetics / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D015151:Immunoblotting / Q000662:veterinary; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; C078167:OMPA outer membrane proteins; D005982:Glutathione Transferase