title: Solution structure of the mEGF/TGFalpha44-50 chimeric growth factor.
abstract: The solution structure of the growth factor chimera mEGF/TGFalpha44-50 has been determined using an extended version of the dyana procedure for calculating structures from NMR data. The backbone fold and preferred orientation of the domains of the chimera are similar to those found in previous studies of EGF structures, and several H-bonds used as input constraints in those studies were found independently in the chimera. This shows that the modified activity of the chimera does not result from a major structural change. However, the improved precision of the structure presented here allows the origin of some unusual chemical shifts found in all of these compounds to be explained, as well as the results obtained from some site-specific mutants. Further studies of the properties of this chimeric growth factor should help to elucidate the mechanism(s) of hetero- and homodimerization of the c-erbB receptors.
authors.affiliation: Cancer Research Campaign Medical Oncology Unit, Southampton General Hospital, Highfield, Southampton, UK.
authors.forename: S G
authors.initials: SG
authors.lastname: Chamberlin
authors.forename: L
authors.initials: L
authors.lastname: Brennan
authors.forename: S M
authors.initials: SM
authors.lastname: Puddicombe
authors.forename: D E
authors.initials: DE
authors.lastname: Davies
authors.forename: D L
authors.initials: DL
authors.lastname: Turner
mesh_terms: D000818:Animals; D019281:Dimerization; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000235:genetics; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012996:Solutions; D016211:Transforming Growth Factor alpha / Q000737:chemistry* / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D012996:Solutions; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor