title: Cytogenetic and molecular characterization of a patient with simultaneous B-cell chronic lymphocytic leukemia and peripheral T-cell lymphoma.
abstract: A patient is described who developed a peripheral T-cell lymphoma (PTCL) after a 6-year history of B-cell chronic lymphocytic leukemia (B-CLL). The progression of the T-cell disease spreading to pleura and skin terminated the course of the disease. A cytogenetic analysis performed six years after the first onset of the B-CLL showed the presence of two clones, one with trisomy 12 and another with inv(14)(q11q32.1) and trisomy 8. Combined immunophenotyping and fluorescence in situ hybridization demonstrated that only CD19+ cells contained a trisomy 12, whereas CD3+ cells contained a trisomy 8. Analyses of IgH and TCR rearrangements in single micromanipulated B- and T-cells lacked evidence for a clonal relation between B-CLL and PTCL cells. Based on our findings, we discuss the different hypotheses which might explain the development of simultaneous PTCL and B-CLL.
authors.affiliation: Institute of Human Genetics, University Hospital Kiel, Germany.
authors.forename: J I
authors.initials: JI
authors.lastname: Martin-Subero
authors.forename: R
authors.initials: R
authors.lastname: Siebert
authors.forename: L
authors.initials: L
authors.lastname: Harder
authors.forename: S
authors.initials: S
authors.lastname: Gesk
authors.forename: P
authors.initials: P
authors.lastname: Schwindt
authors.forename: A
authors.initials: A
authors.lastname: Bräuninger
authors.forename: K
authors.initials: K
authors.lastname: Willenbrock
authors.forename: D
authors.initials: D
authors.lastname: Steinemann
authors.forename: M
authors.initials: M
authors.lastname: Tiemann
authors.forename: M J
authors.initials: MJ
authors.lastname: Calasanz
authors.forename: W
authors.initials: W
authors.lastname: Grote
authors.forename: W D
authors.initials: WD
authors.lastname: Ludwig
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: B
authors.initials: B
authors.lastname: Schlegelberger
mesh_terms: D000368:Aged; D007446:Chromosome Inversion; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020732:Cytogenetic Analysis; D017809:Fatal Outcome; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016411:Lymphoma, T-Cell, Peripheral / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D016609:Neoplasms, Second Primary / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D014314:Trisomy