C-MYC and IGF-II mRNA-binding protein (CRD-BP/IMP-1) in benign and malignant mesenchymal tumors.
Mouse coding region determinant-binding (mCRD-BP) and human IGF-II mRNA-binding 1 (hIMP-1) proteins are orthologous mRNA-binding proteins that recognize c-myc and IGF-II mRNA, respectively, and regulate their expression posttranscriptionally. Here, we confirm that human CRD-BP/IMP-1 binds to c-myc mRNA and that it is predominantly expressed in fetal tissues. Moreover, hCRD-BP/IMP-1 expression was detected in cell lines of neoplastic origin and in selected primary tumors. In a series of 33 malignant and 10 benign mesenchymal tumors, 73% and 40%, respectively, were found to express hCRD-BP/IMP-1. In particular, expression was significant in 14 Ewing's sarcomas, all of which were positive. The data suggest that hCRD-BP/IMP-1 plays a role in abnormal cell proliferation in mesenchymal tumors.
Ioannidis
P
P
Department of Genetics, St. Savas Hospital, Athens, Greece.
Trangas
T
T
Dimitriadis
E
E
Samiotaki
M
M
Kyriazoglou
I
I
Tsiapalis
C M
CM
Kittas
C
C
Agnantis
N
N
Nielsen
F C
FC
Nielsen
J
J
Christiansen
J
J
Pandis
N
N
D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008637:Mesenchymoma / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000378:metabolism; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; C406144:IGF2BP1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012313:RNA