Alkanesulfonate degradation by novel strains of Achromobacter xylosoxidans, Tsukamurella wratislaviensis and Rhodococcus sp., and evidence for an ethanesulfonate monooxygenase in A. xylosoxidans strain AE4.
Novel isolates of Achromobacter xylosoxidans, Tsukamurella wratislaviensis and a Rhodococcus sp. are described. These grew with short-chain alkanesulfonates as their sole source of carbon and energy. T. wratislaviensis strain SB2 grew well with C(3)-C(6) linear alkanesulfonates, isethionate and taurine, Rhodococcus sp. strain CB1 used C(3)-C(10) linear alkanesulfonates, taurine and cysteate, but neither strain grew with ethanesulfonate. In contrast, A. xylosoxidans strain AE4 grew well with ethanesulfonate, making it the first bacterium to be described which can grow with this compound. It also grew with unsubstituted C(3)-C(5) alkanesulfonates and isethionate. Hydrolysis was excluded as a mechanism for alkanesulfonate metabolism in these strains; and evidence is given for a diversity of uptake and desulfonatase systems. We provide evidence for an initial monooxygenase-dependent desulfonation in the metabolism of ethanesulfonate and propanesulfonate by A. xylosoxidans strain AE4.
Erdlenbruch
B N
BN
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Kelly
D P
DP
Murrell
J C
JC
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism*; D000421:Alcaligenes / Q000201:enzymology* / Q000254:growth & development; D000476:Alkanesulfonates / Q000378:metabolism*; D017738:Alkanesulfonic Acids / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D012240:Rhodococcus / Q000254:growth & development / Q000378:metabolism*; D013045:Species Specificity; D013447:Sulfites / Q000378:metabolism
D000476:Alkanesulfonates; D017738:Alkanesulfonic Acids; D013447:Sulfites; C068559:propylsulfonic acid; C002634:ethane sulfonate; D010105:Oxygenases