title: Regulation of cell motility via high and low affinity autocrine motility factor (AMF) receptor in human oral squamous carcinoma cells.
abstract: A tumour-secreted cytokine autocrine motility factor (AMF) induces in vivo invasion and metastasis, and in vitro tumour cell motility by a signal transduction through interaction with its cell surface receptor gp78. In this report, we investigated the characterization of a high-metastatic human oral squamous cell carcinoma (SCC) cell line LMF4 and low-metastatic HSC-3 in comparison with non-metastatic HSC-2 and HSC-4. Morphological and motility analyses revealed LMF4 cells to have the highest motile activity among those cells. However, LMF4 cells shared the similar features with HSC-3: high level secretion of AMF, enhancement of gp78 expression, co-expression of vimentin and cytokeratin, although LMF4 cells showed twice as high motile reactivity as HSC-3. The only difference was that LMF4 had twice as high amount of low-affinity receptor(s) as HSC-3, shown by Scatchard analysis.
authors.affiliation: Tumour Progression and Metastasis Research Program, Karmanos Cancer Institute, 110 East Warren Avenue, Detroit, MI 48201, USA. y-niinaka.mfs@tmd.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Niinaka
authors.forename: A
authors.initials: A
authors.lastname: Haga
authors.forename: A
authors.initials: A
authors.lastname: Negishi
authors.forename: H
authors.initials: H
authors.lastname: Yoshimasu
authors.forename: A
authors.initials: A
authors.lastname: Raz
authors.forename: T
authors.initials: T
authors.lastname: Amagasa
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement / Q000502:physiology*; D016001:Confidence Intervals; D004591:Electrophoresis, Polyacrylamide Gel; D005956:Glucose-6-Phosphate Isomerase / Q000502:physiology*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D060285:Receptors, Autocrine Motility Factor; D018121:Receptors, Cytokine / Q000502:physiology*; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases; D014746:Vimentin / Q000378:metabolism
chemical_list: D018121:Receptors, Cytokine; D014746:Vimentin; D007633:Keratins; C555000:AMFR protein, human; D060285:Receptors, Autocrine Motility Factor; D044767:Ubiquitin-Protein Ligases; D005956:Glucose-6-Phosphate Isomerase