Choline containing metabolites during cell transfection: an insight into magnetic resonance spectroscopy detectable changes.
Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphocholine was detected. This increase appears to be correlated with cell membrane disruption associated with the insertion of plasmid DNA into cells, since the level of phosphocholine in mock transfected cells was comparable to that of control cells. These data suggest choline containing metabolite changes detected in vivo using magnetic resonance spectroscopy relate to cell membrane disruption.
Griffin
J L
JL
Biological Chemistry, Biomedical Sciences, Imperial College of Science, Technology and Medicine, Exhibition Road, South Kensington, London SW7 2AZ, UK. j.griffin@ic.ac.uk
Mann
C J
CJ
Scott
J
J
Shoulders
C C
CC
Nicholson
J K
JK
D002794:Choline / Q000031:analogs & derivatives / Q000378:metabolism*; D005822:Genetic Vectors; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010767:Phosphorylcholine / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D010767:Phosphorylcholine; D002794:Choline