Novel spectral method for the study of viral carcinogenesis in vitro.
Fourier transform infrared (FTIR) spectroscopy is a unique technique for the laboratory diagnosis of cellular variations based on the characteristic molecular vibrational spectra of the cells. Microscopic FTIR was used to investigate spectral differences between normal and malignant fibroblasts transformed by retrovirus infection. A detailed analysis showed significant differences between cancerous and normal cells. The contents of vital cellular metabolites were significantly lower in the transformed cells than in the normal cells. In an attempt to identify the cellular components responsible for the observed spectral differences between normal and cancerous cells, we found significant differences between DNA of normal and cancerous cells.
Huleihel
Mahmoud
M
The Institute for Applied Biosciences, Ben-Gurion University of the Negev, P.O. Box 653, Beersheba 84105, Israel. mahmoudh@bgumail.bgu.ac.il
Salman
Ahmad
A
Erukhimovitch
Vitaly
V
Ramesh
Jagannathan
J
Hammody
Ziad
Z
Mordechai
Shaul
S
D016475:3T3 Cells; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002472:Cell Transformation, Viral / Q000502:physiology*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000472:pathogenicity; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D002241:Carbohydrates; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA