Biodegradable poly (DL-lactide glycolide) microparticles as a vehicle for allergen-specific vaccines: a study performed with Ole e 1, the main allergen of olive pollen.
Ole e 1, the main allergen of the olive pollen, was entrapped in poly (DL-lactide glycolide) microparticles by a solvent evaporation double emulsion technique. The physical properties of the microparticles, such as particle size, surface morphology, allergen entrapment rate and antigen release pattern were investigated. Microparticles with a spherical morphology displayed a size range of less than 2 microm in diameter and antigen loading up to 60-70% (w/w). SDS-PAGE and immunoblotting of the released Ole e 1 confirmed that the molecular integrity and the antigenic properties of Ole e 1 remained unaltered by the encapsulation process or polymer degradation. This finding suggests that microparticles displaying small particle sizes, rapid antigen release and a high allergen/polymer ratio may be a suitable delivery system for antigen in hyposensitization therapy against allergy.
Batanero
Eva
E
Departamento de Bioquímica y Biología Molecular, Facultad de Químicas, Universidad Complutense, 28040 Madrid, Spain.
Barral
Patricia
P
Villalba
Mayte
M
Rodríguez
Rosalía
R
D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology; D052179:Antigens, Plant; D001672:Biocompatible Materials / Q000008:administration & dosage*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000517:prevention & control*; D019344:Lactic Acid / Q000008:administration & dosage* / Q000276:immunology; D010316:Particle Size; D010940:Plant Proteins / Q000008:administration & dosage* / Q000276:immunology; D011058:Pollen; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D022223:Vaccines, Subunit / Q000008:administration & dosage* / Q000276:immunology
D000485:Allergens; D052179:Antigens, Plant; D001672:Biocompatible Materials; D004337:Drug Carriers; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; D011108:Polymers; D022223:Vaccines, Subunit; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid