An inhibitory factor for cell-free protein synthesis from Salmonella enteritidis exhibits cytopathic activity against Chinese hamster ovary cells.
A factor inhibiting cell-free protein synthesis was purified from Salmonella enteritidis cell lysate by sequential ammonium sulfate precipitation, chromatography on anion exchange and hydrophobic interaction columns, and polyacrylamide disc gel electrophoresis. The purified factor, which was named SIPS (Salmonella inhibitor of protein synthesis), inhibited in vitro protein synthesis in rabbit reticulocyte lysate and had a molecular mass of 38 kDa, estimated by PAGE under denaturing conditions. SIPS was also cytopathic for Chinese hamster ovary cells. The N-terminal amino acid sequence (20 residues) of SIPS was found to be identical to that of mature L-asparaginase II of Escherichia coli. Indeed, the purified SIPS exhibited asparaginase activity, E. coli L-asparaginase II had cytopathic activity and inhibited in vitro protein synthesis. The results suggest that at least a part of cytotoxicity and inhibition of cell-free protein synthesis caused by S. enteritidis is a property of the bacterial L-asparaginase.
Iwamaru
Y
Y
Department of Molecular Infectiology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Miyake
M
M
Arii
J
J
Tanabe
Y
Y
Noda
M
M
D000818:Animals; D001215:Asparaginase / Q000302:isolation & purification* / Q000378:metabolism; D016466:CHO Cells / Q000187:drug effects* / Q000473:pathology; D006224:Cricetinae; D003603:Cytotoxins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D008970:Molecular Weight; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits; D012477:Salmonella enteritidis / Q000378:metabolism* / Q000472:pathogenicity
D003603:Cytotoxins; D011500:Protein Synthesis Inhibitors; D001215:Asparaginase