Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation.
Six patients received an allogeneic bone marrow transplant from HLA-identical ABO-mismatched donors. ABO genotype of erythroid burst-forming units (BFU-E) from peripheral blood was analyzed using polymerase chain reaction with sequence specific primers (PCR-SSP). After bone marrow transplantation (BMT), engraftment of donor cells by ABO genotypic analysis of BFU-E was compared with ABO phenotypic analysis of red blood cells (RBCs). During the early stage after BMT, ABO genotype of BFU-E in the recipients converted to that of the donors. In contrast, mixed ABO phenotype of RBCs persisted for about 3 months. In one patient, autologous hemopoietic cell recovery was detected by the ABO genotypic analysis before clinical manifestation. ABO genotypic analysis of BFU-E is relevant for enagraftment after ABO-mismatched BMT.
Kishino
Koji
K
Division of Cell Transplantation and Transfusion, Jichi Medical School, Minamikawachi, 329-0498, Tochigi, Japan.
Muroi
Kazuo
K
Kawano
Chizuru
C
Obata
Takashi
T
Sugano
Naoko
N
Nakagi
Yoko
Y
Nagashima
Takahiro
T
Watari
Kiyoshi
K
Iwamoto
Sadahiko
S
Ozawa
Keiya
K
D000017:ABO Blood-Group System / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D017931:DNA Primers / Q000737:chemistry; D004912:Erythrocytes / Q000166:cytology; D015672:Erythroid Precursor Cells / Q000502:physiology*; D004920:Erythropoiesis; D005434:Flow Cytometry; D005838:Genotype; D006085:Graft Survival; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000628:therapy*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000097:blood / Q000628:therapy*; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000628:therapy*
D000017:ABO Blood-Group System; D017931:DNA Primers