Regeneration of canine peroneal nerve with the use of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge: a comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits.
A novel artificial nerve conduit was developed and its efficiency was evaluated on the basis of promotion of peripheral nerve regeneration across an 80-mm gap in dogs. The nerve conduit was made of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge. Conduits filled with either sponge- or fiber-form collagen were implanted into an 80-mm gap of the peroneal nerve (five dogs for each form). Twelve months postoperatively nerve regeneration was superior in the sponge group both morphometrically (percentage of neural tissue: fiber: 39.7 +/- 5.2, sponge: 43.0 +/- 4.5, n=3) and electrophysiologically (fiber: CMAP 1.06 +/- 0.077, SEP 1.32 +/- 0.127 sponge: CMAP 1.04 +/- 0.106, SEP 1.24 +/- 0.197, n=5), although these differences were not statistically significant. The observed regeneration was complementary to successful results reported previously in the same model, in which collagen fibers exclusively were used. The results indicate a possible superiority of collagen sponge over collagen fibers as filling materials. In addition, the mass-producibility, superior scaffolding potential, and capacity for gradual release of soluble factors of the sponge provide make it an attractive alternative to fine fibers, which are both technologically difficult and costly to produce. This newly developed nerve conduit has the potential to enhance peripheral nerve regeneration across longer gaps commonly encountered in clinical settings.
Toba
T
T
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan. toba@frontier.kyoto-u.ac.jp
Nakamura
T
T
Shimizu
Y
Y
Matsumoto
K
K
Ohnishi
K
K
Fukuda
S
S
Yoshitani
M
M
Ueda
H
H
Hori
Y
Y
Endo
K
K
D000818:Animals; D003094:Collagen / Q000737:chemistry*; D004285:Dogs; D005260:Female; D020007:Implants, Experimental; D007797:Laminin / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009416:Nerve Regeneration; D019635:Neurosurgical Procedures / Q000295:instrumentation*; D010543:Peroneal Nerve / Q000502:physiology*; D011100:Polyglycolic Acid / Q000737:chemistry*; D015503:Surgical Sponges; D023822:Tissue Engineering / Q000295:instrumentation*
D007797:Laminin; D011100:Polyglycolic Acid; D003094:Collagen