[Gly(14)]-Humanin improved the learning and memory impairment induced by scopolamine in vivo.
Humanin is a very recently discovered 24 amino acid linear polypeptide, which protects against cell death induced by either familial Alzheimer's disease mutant of amyloid precursor protein, presenilin-1 or presenilin-2 in vitro. However, it has remained uncertain whether humanin is a useful drug for the animal model of learning and memory deficit. In this study, we evaluated the effects of [Gly(14)]-humanin, a more potent humanin analogue, on the scopolamine HBr (1 mg kg(-1) s.c.)-induced impairment of spontaneous alternation behaviour in the Y-maze, an index of short-term memory in mice. [Gly(14)]-Humanin (1000 pmol 5 microl(-1) i.c.v.) reversed the impairment without affecting the number of arm entries. These results suggest that (I) [Gly(14)]-humanin is a beneficial drug for the impairment of learning and memory and (II) it modulates the learning and memory function mediated via cholinergic systems in mice.
Mamiya
T
T
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Meijo University, 150, Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan. mamiya@ccmfs.meijo-u.ac.jp
Ukai
M
M
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001522:Behavior, Animal / Q000187:drug effects; D003672:Defecation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D047908:Intracellular Signaling Peptides and Proteins; D007859:Learning Disabilities / Q000139:chemically induced / Q000188:drug therapy*; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D012601:Scopolamine / Q000037:antagonists & inhibitors*; D013997:Time Factors
C450880:Gly(14)-Humanin; D047908:Intracellular Signaling Peptides and Proteins; D018727:Muscarinic Antagonists; D018696:Neuroprotective Agents; D010455:Peptides; D011506:Proteins; C427211:humanin; D012601:Scopolamine