Synthesis and characterization of tetramethylrhodaminethiocarbamoyl-(Glu(1))-epidermal mitosis-inhibiting pentapeptide.
A fluorescent analog of epidermal mitosis-inhibiting pentapeptide (pGlu-Glu-Asp-Ser-Gly) was synthesized by reacting tetramethylrhodamine isothiocyanate with ring-opened epidermal mitosis-inhibiting pentapeptide. The ring-opening reaction of the pyrrolidone moiety was performed with mild acidic hydrolysis and the product purified by reversed-phase high-performance liquid chromatography. Tetramethylrhodaminethiocarbamoyl-(Glu(1))-epidermal mitosis-inhibiting pentapeptide was purified by chromatography on Sephadex G-25 and reversed-phase high-performance liquid chromatography. After characterization by amino acid analysis, the analog was incubated in presence of A431 cell line to visualize the cellular localization of the epidermal mitosis-inhibiting pentapeptide. The data gave negative results.
Bramucci
M
M
Department of Molecular, Cellular and Animal Biology, University of Camerino, Via F. Camerini n. 2, I-62032 Camerino (MC), Italy.
Miano
A
A
Quassinti
L
L
Murri
O
O
Amici
D
D
D002448:Cell Adhesion / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes / Q000378:metabolism; D018698:Glutamic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D018613:Microscopy, Confocal; D008938:Mitosis / Q000187:drug effects*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012235:Rhodamines / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D010455:Peptides; D012235:Rhodamines; C448683:tetramethylrhodaminethiocarbamoyl-(Glu(1)); D018698:Glutamic Acid; C009434:tetramethylrhodamine isothiocyanate