title: Expression levels of transdominant peptides and proteins in Saccharomyces cerevisiae.
abstract: From libraries of peptides and protein fragments, several inhibitors that block pheromone response in Saccharomyces cerevisiae have been isolated previously. In many cases, the inhibitors are displayed as part of a scaffold, such as green fluorescent protein. Each of the inhibitors has a characteristic physiological strength or genetic penetrance. In this report, the roles of expression level and display scaffold on the activities of a subset of pheromone-response pathway inhibitors were examined. Special consideration was given to the relationship between expression levels of specific inhibitors, which may exceed 50 microM in some instances, and penetrance.
authors.affiliation: Deltagen Proteomics, Inc., 615 Arapeen Drive, Suite 300, Salt Lake City, UT 84108, USA. Tsandrock@arcanis.com
authors.forename: Tanya
authors.initials: T
authors.lastname: Sandrock
authors.forename: Mark
authors.initials: M
authors.lastname: Poritz
authors.forename: Marianne
authors.initials: M
authors.lastname: Kim
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Feldhaus
authors.forename: Bruce
authors.initials: B
authors.lastname: Roth
authors.forename: Giordano
authors.initials: G
authors.lastname: Caponigro
authors.forename: Alexander
authors.initials: A
authors.lastname: Kamb
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D010675:Pheromones / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D016335:Zinc Fingers
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D005656:Fungal Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; C071657:PEP3 protein, S cerevisiae; D010455:Peptides; D010675:Pheromones; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins