title: Comparison of the effects of radiographic contrast media on dehydration and filterability of red blood cells from donors homozygous for hemoglobin A or hemoglobin S.
abstract: Iodinated radiographic contrast media have traditionally been contraindicated in patients with sickle cell disease because their high osmolality may induce osmotic shrinkage of red blood cells, impair blood flow through the microcirculation, and precipitate or exacerbate a sickle cell crisis. This study investigated that concept by comparing the hematological and rheological effects in vitro of four X-ray contrast media of differing osmolalities: Visipaque (290 mOsm/kg), Hexabrix (600 mOsm/kg), Omnipaque (844 mOsm/kg), and RenoCal-76 (1940 mOsm/kg). Blood was tested from 10 normal and 10 sickle cell donors at drug concentrations of 0, 1, 10, and 30% w/v in an attempt to approximate the relative concentrations of contrast medium to blood that might occur during the bolus-injection and circulation-diluted phases of drug administration. Parameters evaluated included hematology, red cell morphology, and red cell flow resistance through a micropore filter to approximate the microcirculatory effects. Significant hematological effects for both normal and sickle cell donors included a concentration dependent decrease in hematocrit and MCV, and increase in MCHC, all of which varied directly with the osmolality of the contrast media in the order of RenoCal-76 > Omnipaque > Hexabrix > Visipaque. The contrast media had minor effects on red blood cell morphology except for RenoCal-76, 10-30% in which marked echinocytosis was observed. There was no significant increase in the number of irreversibly sickled cells in donors with hemoglobin S. Filterability of red cell suspensions through capillary size pores was impaired in both normal and sickle cell samples in direct proportion to the osmolality of the contrast media, as listed above. Filterability effects were greater for sickle cells than for normal red cells. Visipaque, which was closest to isotonicity, had little effect on red cell volume and had no significant effect on filterability of normal or sickle cells. These results suggest that microcirculatory impairment following infusion of contrast media may occur in sickle patients because of the unusual rheological sensitivity of HbSS red cells, and may be avoided by choice of an isotonic medium.
authors.affiliation: Schering-Plough Research Institute, Lafayette, New Jersey 07848, USA. pat.losco@spcorp.com
authors.forename: P
authors.initials: P
authors.lastname: Losco
authors.forename: G
authors.initials: G
authors.lastname: Nash
authors.forename: P
authors.initials: P
authors.lastname: Stone
authors.forename: J
authors.initials: J
authors.lastname: Ventre
mesh_terms: D000755:Anemia, Sickle Cell / Q000473:pathology*; D001782:Blood Donors; D003287:Contrast Media / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D005374:Filtration; D006400:Hematocrit; D006441:Hemoglobin A; D006451:Hemoglobin, Sickle; D018056:Hemorheology / Q000187:drug effects; D006720:Homozygote; D006801:Humans; D007472:Iohexol / Q000494:pharmacology; D007485:Ioxaglic Acid / Q000494:pharmacology; D009994:Osmolar Concentration; D014283:Triiodobenzoic Acids / Q000494:pharmacology; D014867:Water / Q000378:metabolism
chemical_list: D003287:Contrast Media; D006451:Hemoglobin, Sickle; D014283:Triiodobenzoic Acids; D014867:Water; D007472:Iohexol; D006441:Hemoglobin A; C044834:iodixanol; D007485:Ioxaglic Acid