Microdissection and chromosome painting of plant B chromosomes.
Plant chromosome microdissection techniques together with different isolation and amplification methods of microisolated DNA are described. Such isolated DNA was used to 'chromosome paint' B chromosomes of the dicot Brachycome dichromosomatica and the monocot Secale cereale. It is demonstrated that the specific painting of the described chromosomes was possible because of enrichment for chromosome-specific repetitive sequences, rather than the chromosome specific low- and single-copy sequences which are responsible for the painting of mammalian chromosomes. The feasibility of 'chromosome painting' of standard chromosomes in plant species with relatively small or large genomes is discussed.
Houen
A
A
Department of Genetics, The University of Adelaide, South Australia 5005, Australia. houben@ipk-gatersleben.de
Field
B L
BL
Saunders
V A
VA
D019659:Asteraceae / Q000235:genetics; D020223:Chromosome Painting / Q000379:methods*; D004210:Dissection; D016591:Histocytological Preparation Techniques; D019684:Magnoliopsida / Q000235:genetics*; D012434:Secale / Q000235:genetics