Matrix-bound fibroblasts regulate angiogenesis by modulation of VE-cadherin.
BACKGROUND
Vascular endothelial cadherin (VE-cadherin/cadherin-5) has previously been described as playing a specific role in angiogenesis due to its localisation at areas of intercellular contact, where it functions in maintenance of tubular architecture. Matrix-bound fibroblasts have been show to produce a number of factors that are important in inducing angiogenesis and to promote tubule-formation by human endothelial cells, an indicator of angiogenic potential.


RESULTS
Tubule formation stimulated by fibroblast-derived growth factors can be prevented by the addition of monoclonal antibody to VE-cadherin. In addition, fibroblasts-derived growth factors are able to modulate the expression and hence the regulation of this endothelial cell specific cadherin.


CONCLUSIONS
The change in VE-cadherin expression of human endothelial cells by fibroblast-derived growth factors may contribute to the regulation of angiogenesis.
Martin
T A
TA
Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XN, Wales, UK. Martinta1@cf.ac.uk
Harding
K
K
Jiang
W G
WG
D015703:Antigens, CD; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018919:Neovascularization, Physiologic; D017830:Octoxynol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators; D051176:beta Catenin; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D003598:Cytoskeletal Proteins; D017830:Octoxynol; D015534:Trans-Activators; D051176:beta Catenin; D000099152:Cadherin 5; C495270:CTNNB1 protein, human