title: Particle size analysis of concentrated phospholipid microemulsions I. Total intensity light scattering.
abstract: Water-in-oil phospholipid microemulsions prepared from a constant total surfactant/cosurfactant concentration of 25 wt% at four different lecithin/alcohol weight ratios (Km of 1:1, 1.5:1, 1.77: 1, and 1.94:1) and containing water concentrations (or volume fractions) ranging from 2.0 to 26 wt% (or 0.04 to 0.26) have been examined at 298 K using total intensity light scattering. The data obtained were analysed using the hard-sphere model of Percus-Yevick, modified to account for the partitioning of the alcohol between the various phases. The light-scattering results showed that, regardless of the Km or the alcohol used, a minimum water concentration of at least 9 wt% was required for the formation of a microemulsion; although this value was reasonably constant for each of the alcohols investigated, there was a tendency for a slightly higher concentration of water to be required for microemulsion formation at higher Km values. Simple calculations suggested that a microemulsion was formed only when sufficient water was present to satisfy the hydration of both the phospholipid head groups and the hydroxyl groups of the cosurfactant associated with the droplet. At water concentrations lower than this minimum value, a cosolvent system was observed. In all systems above this minimum concentration, as the concentration of water increased, the size of the microemulsion droplets also increased. Surprisingly, however, there was little difference in the size of the microemulsion droplets obtained with the different alcohols, regardless of the Km, although for a particular alcohol there was some indication that the higher Km systems produced the slightly smaller droplets for an equivalent water concentration. There was also a suggestion that the more hydrophobic alcohols produced slightly smaller droplets than the more polar alcohols at the same Km.
authors.affiliation: Department of Pharmacy, King's College London, Franklin Wilkins Building, 150 Stamford Street, London SE1 8WA, UK.
authors.forename: R
authors.initials: R
authors.lastname: Aboofazeli
authors.forename: D J
authors.initials: DJ
authors.lastname: Barlow
authors.forename: M J
authors.initials: MJ
authors.lastname: Lawrence
mesh_terms: D000440:Butanols; D004655:Emulsions; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D009226:Myristates; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D020005:Propanols; D012542:Scattering, Radiation; D013501:Surface-Active Agents; D014867:Water
chemical_list: D000440:Butanols; D004655:Emulsions; D009226:Myristates; D010713:Phosphatidylcholines; D020005:Propanols; D013501:Surface-Active Agents; D014867:Water; C008205:isopropyl myristate