Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.).
Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with 1% HCl-CH(3)OH, and the crude anthocyanin extract was purified by Shepadex LH-20 and Lichroprep RP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-visible, FABMS, (1)H and (13)C NMR, and by TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-glucoside, cyanidin-3-glucoside, and petunidin-3-glucoside. Among them, petunidin-3-glucoside was identified as a new anthocyanin in black soybeans. On the basis of RP-HPLC with a UV-vis detector, the contents of delphinidin-3-glucoside, cyanidin-3-glucoside, petunidin-3-glucoside, and total anthocyanins in seed coats of 10 black soybeans were found in the ranges of 0-3.71, 0.94-15.98, 0-1.41, and 1.58-20.18 mg/g, respectively. The results obtained in this study imply that the seed coats of black soybean can be used as a good source for cyanidin-3-glucoside and delphinidin-3-glucoside.
Choung
M G
MG
National Yeongnam Agricultural Experiment Station, Rural Development Administration, 1085, Naidong, Milyang, 627-803, Gyeongnam, Republic of Korea. cmg700@rda.go.kr
Baek
I Y
IY
Kang
S T
ST
Han
W Y
WY
Shin
D C
DC
Moon
H P
HP
Kang
K H
KH
D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D012639:Seeds / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
D000872:Anthocyanins