Antistress effect of oligosaccharides extracted from Morinda officinalis in mice and rats.
OBJECTIVE
To explore the antistress effect of Morinda officinalis (Chinese medicine "Bajitian") oligosaccharides (MW-97) in mice.


METHODS
Male mice and rats were subjected to a variety of unpredictable stressors on a daily basis over 15 d period and then the Vidiomex-V image pattern analytic system was used to observe the spontaneous motor activity. Meanwhile, regular method was used to prepare pathologic section of adrenal gland and blood cells analytic system was used to detect white blood cell (WBC) count (including relative WBC percentages) in peripheral blood. On the end, the serum level of stress hormone was detected using radioimmunoassay.


RESULTS
Chronic stress resulted in diffuse hyperplasia of the adrenal cortex and atrophy of the adrenal medulla in mice, which suggested that stress-adaption failure of the adrenal gland occurred, while adrenal gland of the mice pretreated with MW-97 (100 mg/kg, ip) prior to each stressor for 15 d did not occur any pathologic changes. In addition, chronic stress also significantly reduced the WBC count and relative WBC percentages in the peripheral blood, including the percentage of lymphocytes, monocytes, neutrophils, however, MW-97 (25 and 100 mg/kg) reversed these changes and raised WBC count, along with relative WBC percentages significantly. Furthermore, the serum concentration of testosterone was decreased and corticosterone was increased significantly in chronically stressed animals. MW-97 also declined the serum level of corticosterone and raised level of testosterone. MW-97 had no effects on the spontaneous motor activity in the stressed mice.


CONCLUSIONS
MW-97 had antistress effect against chronic stress, moreover, MW-97 had no excitatory or inhibitory effects on the CNS, which suggested that MW-97 might become a new kind of antistress agent.
Li
Y F
YF
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. LYF619@yahoo.com.cn
Yuan
L
L
Xu
Y K
YK
Yang
M
M
Zhao
Y M
YM
Luo
Z P
ZP
D000311:Adrenal Glands / Q000187:drug effects* / Q000473:pathology; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D032066:Morinda / Q000737:chemistry*; D009043:Motor Activity / Q000187:drug effects*; D009844:Oligosaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism; D013739:Testosterone / Q000097:blood
D014151:Anti-Anxiety Agents; D009844:Oligosaccharides; D013739:Testosterone; D003345:Corticosterone