The mouse enhancer element D6 directs Cre recombinase activity in the neocortex and the hippocampus.
We have created a transgenic mouse line that expresses Cre recombinase under the control of the novel mouse promoter/enhancer D6. We describe the expression pattern of D6-Cre in a Gtrosa26 reporter background as assayed by LacZ activity. The enhancer activity starts at 10.5 days post-coitum in the telencephalon and is at the later embryonic stages highly restricted to the hippocampus and the neocortex. In adult mice D6-derived cells are found in cortical layers II-VI, in the granular cells of the dentate gyrus and in hippocampal fields CA1-CA3. D6-Cre activity is also detected in the ependymal and subependymal zone of the lateral ventricles which is known to harbor neural stem cells.
van den Bout
Christiaan J
CJ
Section for Genetic Therapy, Institute for Microbiology, The National Hospital, 0027 Oslo, Norway.
Machon
Ondrej
O
Røsok
Øystein
Ø
Backman
Mattias
M
Krauss
Stefan
S
D000818:Animals; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D006624:Hippocampus / Q000196:embryology* / Q000201:enzymology* / Q000254:growth & development; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics*; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D019579:Neocortex / Q000196:embryology* / Q000201:enzymology* / Q000254:growth & development; D009474:Neurons / Q000201:enzymology; D013234:Stem Cells / Q000201:enzymology; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases