Zic2 controls cerebellar development in cooperation with Zic1.
Mouse Zic genes encode zinc finger proteins and are expressed in the developing and mature CNS. Reduced expression of Zic2 in mice results in spina bifida and holoprosencephaly. However, the disruption of Zic1, a strong homolog of Zic2 that has an overlapping expression pattern, results in cerebellar malformation with no apparent abnormalities in the forebrain or in posterior neuropore closure. Here we revealed that Zic2 and Zic1 cooperatively control cerebellar development by regulating neuronal differentiation. Both Zic1 and Zic2 are expressed in the precursor cells of the granule neuron and the neurons in cerebellar nuclei. Mice carrying one mutated Zic1 allele together with one mutated Zic2 allele (Zic1(+/-)Zic2(+/kd)) showed a marked cerebellar folial abnormality similar to, but distinct from that found in mice homozygous for the Zic1 mutation (Zic1(-/-)). The Zic1(+/-)Zic2(+/kd) cerebellum is missing a lobule in the anterior vermis and has a truncation of the most posterior lobule. Expression of transverse zonal markers is shifted anteriorly in the developing cerebellum, indicating that the anterior part of the cerebellum is poorly developed. Abnormalities in the developing Zic1(+/-)Zic2(+/kd) cerebellum share the following features with those of the Zic1(-/-) cerebellum: a preceding reduction of cell proliferation in the anterior external germinal layer, a reduction in cyclin D1 expression, and enhanced expression of the mitosis inhibitors p27 and p16, and enhancement of Wnt7a expression. These results indicate that Zic1 and Zic2 may have very similar functions in the regulation of cerebellar development.
Aruga
Jun
J
Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, Wako-shi, Saitama 351-0198, Japan. jaruga@brain.riken.go.jp
Inoue
Takashi
T
Hoshino
Jun
J
Mikoshiba
Katsuhiko
K
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002531:Cerebellum / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology*; D019938:Cyclin D1 / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006579:Heterozygote; D006720:Homozygote; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008818:Mice, Neurologic Mutants; D009024:Morphogenesis / Q000235:genetics; D009154:Mutation; D009421:Nervous System Malformations / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009928:Organ Specificity; D010641:Phenotype; D013234:Stem Cells / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism
D014157:Transcription Factors; C492122:Zic1 protein, mouse; C491811:Zic2 protein, mouse; D019938:Cyclin D1