title: Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis.
abstract: Axon outgrowth during development and neurotransmitter release depends on exocytotic mechanisms, although what protein machinery is common to or differentiates these processes remains unclear. Here we show that the neural t-SNARE (target-membrane-associated-soluble N-ethylmaleimide fusion protein attachment protein (SNAP) receptor) SNAP-25 is not required for nerve growth or stimulus-independent neurotransmitter release, but is essential for evoked synaptic transmission at neuromuscular junctions and central synapses. These results demonstrate that the development of neurotransmission requires the recruitment of a specialized SNARE core complex to meet the demands of regulated exocytosis.
authors.affiliation: Department of Neurosciences, University of New Mexico Health Science Center, Albuquerque, New Mexico 87131, USA.
authors.forename: Philip
authors.initials: P
authors.lastname: Washbourne
authors.forename: Peter M
authors.initials: PM
authors.lastname: Thompson
authors.forename: Mario
authors.initials: M
authors.lastname: Carta
authors.forename: Edmar T
authors.initials: ET
authors.lastname: Costa
authors.forename: James R
authors.initials: JR
authors.lastname: Mathews
authors.forename: Guillermina
authors.initials: G
authors.lastname: Lopez-Benditó
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Molnár
authors.forename: Mark W
authors.initials: MW
authors.lastname: Becher
authors.forename: C Fernando
authors.initials: CF
authors.lastname: Valenzuela
authors.forename: L Donald
authors.initials: LD
authors.lastname: Partridge
authors.forename: Michael C
authors.initials: MC
authors.lastname: Wilson
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D005089:Exocytosis / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000502:physiology; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D050600:SNARE Proteins; D009435:Synaptic Transmission / Q000502:physiology*; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; C494209:Snap25 protein, mouse; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins