A monomeric mannose-binding lectin from inner shoots of the edible chive (Allium tuberosum).
A mannose-binding lectin was isolated from the inner shoots of the chive Allium tuberosum. The procedure involved aqueous extraction, (NH4)2SO4 precipitation, dialysis to remove (NH4)2SO4, affinity chromatography on mannose-agarose, ion exchange chromatography on SP-Sepharose, gel filtration on Superdex 75, and ion exchange chromatography on Mono S. Lectin activity was adsorbed on mannose-agarose, SP-Sepharose, and Mono S. The lectin demonstrated a molecular weight of 13 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration, indicating that it is a single-chain protein. N-terminal sequence analysis revealed its remarkable homology to Allium cepa lectin and similarity to a lesser extent to lectins from members of the Amaryllidaceae, Orchidaceae, and Liliaceae. The lectin manifested mitogenic activity in murine splenocytes and inhibitory activity against human immunodeficiency virus type 1 reverse transcriptase.
Lam
Y W
YW
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong.
Ng
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D027782:Chive / Q000378:metabolism*; D037222:Collectins; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006384:Hemagglutination / Q000187:drug effects; D037102:Lectins / Q000302:isolation & purification* / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D037121:Plant Lectins; D018520:Plant Shoots / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D002352:Carrier Proteins; D037222:Collectins; D037102:Lectins; D008934:Mitogens; D037121:Plant Lectins; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D008358:Mannose