Kinetic studies during peripheral blood stem cell collection show CD34+ cell recruitment intra-apheresis.
A sufficient number of CD34+ cells in the peripheral blood stem cell product is important to achieve a rapid and sustained engraftment. The purpose of the present work was to study CD34+ cell kinetics during leukapheresis. Blood samples before and after leukapheresis were analysed for CD34+ cells in 205 procedures. The number of CD34+ cells after plus the number of CD34+ cells harvested was 1.5-fold greater than the number available at the beginning of the procedure, indicating recruitment of CD34+ cells during leukapheresis. In a subgroup of 66 procedures, granulocytes and platelets were measured. In contrast to CD34+ cells, these cell fractions were not recruited to the blood stream during leukapheresis. An additional nine patients were studied with serial blood measurements during leukapheresis, showing an initial decline that was followed by an increase in CD34+ cells during leukapheresis. In conclusion, CD34+ cells are recruited to the blood during the leukapheresis procedure in contrast to granulocytes and platelets.
Knudsen
L M
LM
Department of Haematology L, Herlev Hospital, University of Copenhagen, Herlev, Denmark. l.meldgaard@dadlnet.dk
Nikolaisen
K
K
Gaarsdal
E
E
Johnsen
H E
HE
D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis*; D001772:Blood Cell Count; D002465:Cell Movement; D015331:Cohort Studies; D025461:Feedback, Physiological; D005260:Female; D019337:Hematologic Neoplasms / Q000628:therapy; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D007937:Leukapheresis / Q000592:standards*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology
D018952:Antigens, CD34