title: Novel spectral method for the study of viral carcinogenesis in vitro.
abstract: Fourier transform infrared (FTIR) spectroscopy is a unique technique for the laboratory diagnosis of cellular variations based on the characteristic molecular vibrational spectra of the cells. Microscopic FTIR was used to investigate spectral differences between normal and malignant fibroblasts transformed by retrovirus infection. A detailed analysis showed significant differences between cancerous and normal cells. The contents of vital cellular metabolites were significantly lower in the transformed cells than in the normal cells. In an attempt to identify the cellular components responsible for the observed spectral differences between normal and cancerous cells, we found significant differences between DNA of normal and cancerous cells.
authors.affiliation: The Institute for Applied Biosciences, Ben-Gurion University of the Negev, P.O. Box 653, Beersheba 84105, Israel. mahmoudh@bgumail.bgu.ac.il
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Huleihel
authors.forename: Ahmad
authors.initials: A
authors.lastname: Salman
authors.forename: Vitaly
authors.initials: V
authors.lastname: Erukhimovitch
authors.forename: Jagannathan
authors.initials: J
authors.lastname: Ramesh
authors.forename: Ziad
authors.initials: Z
authors.lastname: Hammody
authors.forename: Shaul
authors.initials: S
authors.lastname: Mordechai
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002472:Cell Transformation, Viral / Q000502:physiology*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000472:pathogenicity; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000737:chemistry / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D002241:Carbohydrates; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA