Bioengineering and characterization of DNA-protein assemblies floating on supported membranes.
A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors.
Boireau
Wilfrid
W
Laboratoire d'lngénierie des Protéines Membranaires, Centre de Génétique Moléculaire, CNRS, F-91198 Gif-sur-Yvette Cedex, France.
Bombard
Sophie
S
Sari
Marie-Agnès
MA
Pompon
Denis
D
D001483:Base Sequence; D002846:Chromatography, Affinity; D017931:DNA Primers; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D015202:Protein Engineering; D020349:Surface Plasmon Resonance
D017931:DNA Primers; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D008567:Membranes, Artificial