Partial identification of the mab (CD)Amp1 antigen at the epithelial-mesenchymal interface in the developing kidney.
The nature of the primary functional events of nephron induction is still unknown, making it impossible to completely understand the mechanism of tissue interaction between collecting duct ampulla and the surrounding nephrogenic mesenchyme. Soluble morphogenic substances are known to be exchanged in the process and it is assumed that nephron induction requires close contact between both tissues involved. Contrasting with that assumption our previous investigation revealed a thick fibrous meshwork separating nephron inducer and mesenchyme. Our present investigation focused on the molecular characterization of the mab (CD)Amp1 antigen, which is found only in this meshwork. The protein was shown immunohistochemically to be located exclusively at the embryonic collecting duct ampulla and could be clearly distinguished from other extracellular matrix proteins such as collagen type IV, laminin, reticulin, and fibronectin. Two-dimensional electrophoresis of the soluble form of P(CD)Amp1 showed a molecular weight of 87,000 and an isoelectric point of 4.3-4.4. Results from N-terminal sequencing indicated a partial sequence homology of P(CD)Amp1 to collagen type IV alpha 2-chain precursor but additionally yielded unknown sequences. Thus P(CD)Amp1 is a novel, collagen-related protein, restricted to the fibrous meshwork at the mesenchymal-epithelial interphase, which is the site of primary epithelial-mesenchymal interaction.
Strehl
R
R
Department of Anatomy, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany.
Minuth
W W
WW
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000737:chemistry / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000737:chemistry* / Q000166:cytology; D005109:Extracellular Matrix / Q000737:chemistry; D016326:Extracellular Matrix Proteins / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D007685:Kidney Tubules, Collecting / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D024141:Collagen Type IV; D016326:Extracellular Matrix Proteins