title: Adrenodoxin-cytochrome P450scc interaction as revealed by EPR spectroscopy: comparison with the putidaredoxin-cytochrome P450cam system.
abstract: The cholesterol side-chain cleavage reaction catalyzed by cytochrome P450scc comprises three consecutive monooxygenase reactions (22R-hydroxylation, 20S-hydroxylation, and C(20)-C(22) bond scission) that produces pregnenolone. The electron equivalents necessary for the oxygen activation are supplied from a 2Fe-2S type ferredoxin, adrenodoxin. We found that 1:1 stoichiometric binding of oxidized adrenodoxin to oxidized cytochrome P450scc complexed with cholesterol or 25-hydroxycholesterol caused shifts of the high-spin EPR signals of the heme moiety at 5 K. Such shifts were not observed for the low-spin EPR signals. Ligation of CO or NO to the reduced heme of cytochrome P450scc complexed with reduced adrenodoxin and various steroid substrates did not cause any change in the axial EPR spectrum of the reduced iron-sulfur center at 77 K. These results are in remarkable contrast to those obtained for the cytochrome P450cam-d-camphor-putidaredoxin ternary complex, suggesting that the mode of cross talk between adrenodoxin and cytochrome P450scc is very different from that in the Pseudomonas system. The difference may be primarily due to the location of the charged amino acid residues of the ferredoxins important for the interaction with the partner cytochrome P450.
authors.affiliation: Department of Life Science, Faculty of Science, Himeji Institute of Technology, Kamigoori-cho, Akou-gun, Hyogo 678-1297, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Tsubaki
authors.forename: J
authors.initials: J
authors.lastname: Futagawa
authors.forename: F
authors.initials: F
authors.lastname: Masuya
authors.forename: H
authors.initials: H
authors.lastname: Hori
mesh_terms: D000302:Adrenal Cortex; D000325:Adrenodoxin / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D019475:Camphor 5-Monooxygenase / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005288:Ferredoxins / Q000378:metabolism*; D006418:Heme / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D016958:Pseudomonas putida / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005288:Ferredoxins; D000325:Adrenodoxin; D009569:Nitric Oxide; D006418:Heme; C100137:putidaredoxin; D002248:Carbon Monoxide; D007501:Iron; D019475:Camphor 5-Monooxygenase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D010100:Oxygen