title: Staurosporine treatment and serum starvation promote the cleavage of emerin in cultured mouse myoblasts: involvement of a caspase-dependent mechanism.
abstract: Emerin is a nuclear membrane-anchored protein which is absent or mutated in patients affected by Emery-Dreifuss muscular dystrophy. In this study, we induced apoptosis in cultured mouse myoblasts to evaluate emerin fate during the nuclear destabilization involved in programmed cell death. Emerin proteolysis was observed in myocytes during the apoptotic process. Myoblast apoptosis and emerin degradation were associated with chromatin compaction and detachment from the nuclear lamina, as detected by electron microscopy. In vivo specific inhibition of caspase 3 or caspase 6 activity completely abolished emerin proteolysis. These results show that the process of programmed cell death in muscle cells leads to emerin proteolysis, which appears to be related to caspase 6 activation and to cleavage of other nuclear envelope proteins, that share sequence homologies or functional features with emerin.
authors.affiliation: Laboratory of Neuromuscular Pathology, IOR, Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Columbaro
authors.forename: E
authors.initials: E
authors.lastname: Mattioli
authors.forename: G
authors.initials: G
authors.lastname: Lattanzi
authors.forename: C
authors.initials: C
authors.lastname: Rutigliano
authors.forename: A
authors.initials: A
authors.lastname: Ognibene
authors.forename: N M
authors.initials: NM
authors.lastname: Maraldi
authors.forename: S
authors.initials: S
authors.lastname: Squarzoni
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D002460:Cell Line; D016895:Culture Media, Serum-Free; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D019311:Staurosporine / Q000494:pharmacology*; D013946:Thymopoietins / Q000378:metabolism*; D013997:Time Factors
chemical_list: D061945:Caspase Inhibitors; D016895:Culture Media, Serum-Free; D008565:Membrane Proteins; D009687:Nuclear Proteins; D013946:Thymopoietins; C092710:emerin; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D019311:Staurosporine