Multicolor fluorescence in situ hybridization in clinical cytogenetic diagnostics.
Multicolor fluorescence in situ hybridization is a technology that has vastly expanded the diagnostic repertoire of the clinical cytogenetics laboratory. The limitations of conventional chromosome banding analysis can often be overcome by the high sensitivity and specificity of multicolor fluorescence in situ hybridization tests. This article reviews the latest multicolor fluorescence in situ hybridization tests (including multiplex fluorescence in situ hybridization, spectral karyotyping, cross-species color banding, and comparative genomic hybridization) that are currently limited to a few select clinical cytogenetic laboratories, but may soon have more dominant roles in clinical cytogenetic practice.
Lee
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C
Department of Pathology, Brigham and Women's Hospital, Boston Massachusetts, USA. clee@rics.bwh.harvard.edu
Lemyre
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E
Miron
P M
PM
Morton
C C
CC
D000818:Animals; D002871:Chromosome Banding; D002872:Chromosome Deletion; D020223:Chromosome Painting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping