Fluidized bed design parameters affecting novel lactic Acid downstream processing.
Lactic acid purification was directly done from fermentation utilizing a fluidized bed column refilled with a strong anionic exchange resin. The purpose of this work was to study the influence of two important design parameters, bed-diameter (D) and bed-height (H), in the lactic acid binding and elution capacity of the matrix. By changing the settled bed height from 2.5 to 5 cm for each diameter of column analyzed it was possible to obtain an 50% increase in the binding capacity of the resin in all experiments. This fact was attributed to a higher contact time between the culture broth and the anionic resin produced by the increase of back mixing and lactic acid residence time.
Sosa
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AV
PROIMI-Biotecnología, Av. Belgrano y Pje. Caseros, (4000) SM Tucumán, Argentina. anasosa@uol.com.br
Córdoba
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PR
Perotti
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D000327:Adsorption; D000465:Algorithms; D000837:Anion Exchange Resins; D001431:Bacteriological Techniques; D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D019344:Lactic Acid / Q000302:isolation & purification / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism; D012117:Resins, Synthetic
D000837:Anion Exchange Resins; D003470:Culture Media; D012117:Resins, Synthetic; D019344:Lactic Acid; C046797:amberlite