title: Comparative properties of two peptide-antibody interactions as deduced from epitope delineation.
abstract: The linear epitope recognized by three closely related antibodies specific for the E6 oncoprotein of papillomavirus type 16 was delineated by phage display, spot peptide synthesis on cellulose membranes, and kinetic measurements with antigenic variants using a BIACORE. The same approaches, recently applied to an antibody specific for tobacco mosaic virus protein, led to the clear-cut delineation of a functional epitope comprising four key positions with well defined physico-chemical properties. In contrast, the E6 system is characterized by a non-essential contribution to binding of various factors, so that combinations of alternative properties are compatible with measurable binding activity.
authors.affiliation: FRE2370-CNRS, Biotechnologie des Interactions Mol., Ecole Superieure de Biotech. de Strasbourg (ESBS), Boulevard Sébastien Brandt, 67400 Illkirch Cedex, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Choulier
authors.forename: Georges
authors.initials: G
authors.lastname: Orfanoudakis
authors.forename: Philippe
authors.initials: P
authors.lastname: Robinson
authors.forename: Daniel
authors.initials: D
authors.lastname: Laune
authors.forename: Myriam
authors.initials: M
authors.lastname: Ben Khalifa
authors.forename: Claude
authors.initials: C
authors.lastname: Granier
authors.forename: Etienne
authors.initials: E
authors.lastname: Weiss
authors.forename: Danièle
authors.initials: D
authors.lastname: Altschuh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000937:Antigen-Antibody Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006801:Humans; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D019151:Peptide Library; D010455:Peptides