title: Immunohistochemical staining of lentigo maligna during Mohs micrographic surgery using MART-1.
abstract: BACKGROUND
  Lentigo maligna (LM) often displays extensive subclinical spread. Mohs micrographic surgery (MMS) has been proposed to help delineate the true histologic margin; however, visualizing atypical melanocytes on frozen section is challenging and often requires confirmatory permanent paraffin sections.
  
  
  OBJECTIVE
  Our aim was to use a monoclonal antibody to rapidly stain frozen sections during MMS to facilitate better visualization of atypical melanocytes.
  
  
  METHODS
  Frozen sections of LM during MMS were stained with MART-1 (melanoma antigen recognized by T cells) and compared with paraffin-embedded sections.
  
  
  RESULTS
  We found 100% correlation between frozen sections stained with MART-1 and paraffin-embedded sections.
  
  
  CONCLUSIONS
  Atypical melanocytes can be better visualized on frozen sections of LM by using MART-1 rather than hematoxylin and eosin. This allows for easier identification during MMS and better chance of complete removal of LM lesions.
authors.affiliation: Department of Dermatologic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Larisa C
authors.initials: LC
authors.lastname: Kelley
authors.forename: Laurie
authors.initials: L
authors.lastname: Starkus
mesh_terms: D000951:Antigens, Neoplasm; D016022:Case-Control Studies; D006801:Humans; D018327:Hutchinson's Melanotic Freckle / Q000473:pathology* / Q000601:surgery*; D007150:Immunohistochemistry; D058965:MART-1 Antigen; D015580:Mohs Surgery / Q000379:methods*; D009363:Neoplasm Proteins; D016612:Paraffin Embedding; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery*; D013048:Specimen Handling
chemical_list: D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins