Endogenous production of protoporphyrin IX induced by 5-aminolevulinic acid in leukemia cells.
OBJECTIVE
To explore the photosensitization of 5-aminolevulinic acid (ALA) in myeloid leukemia cell line.


METHODS
Using the technique of fluorescence spectra, the A LA induced protoporphyrin IX (Pp IX) was measured in myeloid leukemia JCS cells. Cofocal laser scanning microscopy (CLSM) combined with fluorescence organelle probe was used to detect the localization of Pp IX in JCS cells at the subcellular levels. MTT assay was used to measure the cell survival after light irradiation.


RESULTS
ALA successfully produced endogenous PpI X in leukemia JCS cells. PpIX was observed to be distributed in the cytoplasm and mitochondria was exhibited as the one of binding sites of PpIX. As a photosensitizer, PpIX initiated photodynamic reaction after light irradiation and effectively photodamaged leukemia cells.


CONCLUSIONS
ALA-based photosensitization could be used for inactivation of leukemia cells.
Chen
J Y
JY
Department of Physics, Fudan University, Shanghai 200433, China. jychen@fudan.edu.cn.
Mak
N Q
NQ
Cheung
N H
NH
Leung
R N
RN
Peng
Q
Q
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX