title: Contribution of flagella and invasion proteins to pathogenesis of Salmonella enterica serovar enteritidis in chicks.
abstract: To explore the relative contribution that flagella and Salmonella invasion proteins make to the virulence of Salmonella enteritidis in poultry, 20-day-old chicks were challenged orally and by subcutaneous injection with wild-type strain SE-HCD, two non-flagellated mutants (fliC::Tn10 mutant and flhD::Tn10 mutant) and two Salmonella invasion protein insertion mutants (sipD and iacP). When injected subcutaneously, wild-type SE-HCD was the only strain to cause substantial mortality and morbidity and to grow well in organs. The flhD mutant of SE-HCD was invasive when given orally, whereas wild-type SE-HCD and the fliC mutant were significantly attenuated. Salmonella invasion protein mutants were not invasive by either route. These results suggest that temporary suppression of Class I regulators of flagellin biosynthesis may aid oral infection in poultry.
authors.affiliation: United States Department of Agriculture, ARS-SEPRL, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: C T
authors.initials: CT
authors.lastname: Parker
authors.forename: J
authors.initials: J
authors.lastname: Guard-Petter
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005407:Flagella / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins; D009154:Mutation; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C107188:SspD protein, Salmonella typhimurium; D015534:Trans-Activators; C090553:flhD protein, E coli; D005408:Flagellin