[The effect of metronidazole on stimulation of adhesion molecule expression on the surface of vascular endothelial cells by Bacteroides fragilis endotoxins and enterotoxin].
The influence of metronidazole on the level of expression of adhesion molecules ICAM-1, VCAM-1 and E-selectin on the surface of vascular endothelial cells activated with B. fragilis endotoxins and enterotoxin was examined. Three enterotoxigenic (ETBF) strains and one nonenterotoxigenic (NTBF) strain were used for lipopolysaccharide extraction. Enterotoxin was prepared from the culture supernatant of the reference B. fragilis ATCC 43858 strain. Expression of adhesion molecules on vascular endothelial cells (HMEC-1 cell line) was determined after their stimulation with bacterial compounds at the concentration of 10 micrograms/ml in the presence of metronidazole at the concentration of 4 micrograms/ml. Endothelial cells were activated for 4 hours (E-selectin expression) and for 24 hours (ICAM-1 and VCAM-1 expression). Adhesion molecules were detected in immunoenzymatic test (ELISA) with mouse, monoclonal antibodies against human ICAM-1, VCAM-1 and E-selectin. The results of experiments suggest, that metronidazole enhances the expression of examined adhesion molecules on endothelial cells. This antimicrobial agent causes some changes in the expression of endothelial ICAM-1, VCAM-1 and E-selectin stimulated by B. fragilis endotoxins and enterotoxin.
Meisel-Mikołajczyk
F
F
Katedra i Zakład Mikrobiologii Lekarskiej AM w Warszawie.
Rokosz
A
A
Kot
K
K
Zawidzka
E
E
Malchar
C
C
Nowaczyk
M
M
Górski
A
A
D001427:Bacterial Toxins / Q000494:pharmacology*; D019040:E-Selectin / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000382:microbiology; D004731:Endotoxins / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000494:pharmacology*; D008795:Metronidazole / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D001427:Bacterial Toxins; D019040:E-Selectin; D004731:Endotoxins; D004768:Enterotoxins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D008795:Metronidazole; C405031:Bacteroides fragilis toxin; D008666:Metalloendopeptidases