title: Low incidence of SV40-like sequences in ependymal tumours.
abstract: Between 1955 and 1963, millions of children and adults were exposed to SV40-contaminated poliovirus vaccines. The oncogenic potential of this polyomavirus was revealed when intracerebral inoculation of SV40 into newborn hamsters resulted in the development of ependymomas and choroid plexus papillomas. Subsequently, SV40-like sequences were repeatedly detected in human ependymomas with broadly ranging incidence rates of 7-90%. Most epidemiological studies, however, have not described an increased occurrence of ependymomas. To gain more data on this controversial issue, this study examined 62 archived ependymal tumours from 31 children and 31 adults who underwent surgery between 1990 and 1999. Only three (5%) of the tumours--including 24 classical, 20 anaplastic, and 12 myxopapillary ependymomas; one subependymoma; and five ependymoblastomas--revealed subgenomic SV40 sequences. None of the ependymomas in patients born between 1920 and 1960 demonstrated SV40-like sequences. The positive tumours represent 7% of grade II and III ependymomas (two paediatric and one adult tumour). DNA sequencing of the PCR product revealed identical sequences of SV40 in the positive ependymal tumours. Compared with the results from other countries, this incidence rate is relatively low. Therefore, it seems likely that significant differences between individual countries exist regarding the prevalence of SV40-positive ependymomas. These differences may reflect different degrees of exposure to SV40-contaminated polio vaccine.
authors.affiliation: Department of Pathology, University Hospitals of Ulm, Ulm, Germany.
authors.forename: F J
authors.initials: FJ
authors.lastname: Reuther
authors.forename: J
authors.initials: J
authors.lastname: Löhler
authors.forename: J
authors.initials: J
authors.lastname: Herms
authors.forename: H H
authors.initials: HH
authors.lastname: Hugo
authors.forename: C
authors.initials: C
authors.lastname: Schindler
authors.forename: F
authors.initials: F
authors.lastname: Leithäuser
authors.forename: I
authors.initials: I
authors.lastname: Melzner
authors.forename: P
authors.initials: P
authors.lastname: Möller
authors.forename: S
authors.initials: S
authors.lastname: Scheil
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D004340:Drug Contamination; D004806:Ependymoma / Q000453:epidemiology / Q000821:virology*; D005858:Germany / Q000453:epidemiology; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008875:Middle Aged; D023321:Poliovirus Vaccines; D016133:Polymerase Chain Reaction / Q000379:methods; D013539:Simian virus 40 / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral; D023321:Poliovirus Vaccines