Involvement of endometrial membrane sulphydryl groups in blastocyst implantation: sulphydryl groups as a potential target for contraceptive research.
The role of membrane sulphydryl groups in blastocyst implantation was studied by masking the membrane sulphydryl groups in the endometrium of Swiss albino mice, Mus musculus, using 10(-5) M cobalt chloride and 0.05 mM as well as 0.005 mM n-ethylmaleimide. Here we show that the blocking of sulphydryl groups with cobalt resulted in a decrease in superoxide radical surge and an increase in superoxide dismutase levels at the time of implantation. We hypothesize that it may be due to either a decrease in membrane fluidity or the unavailability of sulphydryl groups of endometrial membrane, thus preventing blastocyst implantation. These sulphydryl groups can be targeted for future contraceptive research.
Nivsarkar
M
M
Department of Pharmacology and Toxicology, B.V. Patel Pharmaceutical Education and Research Development Centre, Thaltej Gandhinagar Highway, Thaltej, Ahmedabad-380054, Gujarat, India. manishnivsarkar@yahoo.com
Sethi
A
A
Bapu
C
C
Patel
M
M
Padh
H
H
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003035:Cobalt / Q000378:metabolism; D003267:Contraception; D010064:Embryo Implantation / Q000502:physiology*; D004717:Endometrium / Q000502:physiology*; D005260:Female; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D011247:Pregnancy; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013858:Thiobarbiturates / Q000032:analysis
D013438:Sulfhydryl Compounds; D013858:Thiobarbiturates; D013481:Superoxides; D003035:Cobalt; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C029684:thiobarbituric acid