title: Secretion of the Escherichia coli K-12 SheA hemolysin is independent of its cytolytic activity.
abstract: The Escherichia coli K-12 sheA gene encodes a pore-forming hemolysin that is secreted to the medium by a hitherto unidentified mechanism. To study SheA secretion, we constructed fusions between SheA and the mature form of the periplasmic enzyme beta-lactamase, and performed site-directed mutagenesis on these constructs. The SheA-Bla and Bla-SheA hybrid proteins displayed hemolytic activity and were efficiently exported to the extracellular medium. Our results with mutant hybrid proteins show that secretion of SheA is independent of its cytolytic activity, that secretion is paralleled by a transient leakage of periplasmic contents to the extracellular medium, and that deletion of the 11 C-terminal residues of SheA has no effect on its secretion and cytolytic activity.
authors.affiliation: Unidad de Genética Molecular, Hospital Ramón y Cajal, Carretera de Colmenar Km 9, 28034, Madrid, Spain. idelcastillo@hrc.insalud.es
authors.forename: F J
authors.initials: FJ
authors.lastname: del Castillo
authors.forename: F
authors.initials: F
authors.lastname: Moreno
authors.forename: I
authors.initials: I
authors.lastname: del Castillo
mesh_terms: D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism* / Q000633:toxicity; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C119944:hlyE protein, E coli; D001618:beta-Lactamases