title: Changes in concentrations of type IV collagen and tissue inhibitor of metalloproteinase-1 in patients with paraquat poisoning.
abstract: Respiratory failure is one of the major causes of death in patients with paraquat poisoning. In paraquat-poisoned lungs, abnormal extracellular matrix regulation occurs. The aim of the present study is to determine whether serum concentrations of type IV collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) are altered during the course of paraquat poisoning and whether haemoperfusion therapy affects these concentrations. Twenty-one patients were admitted within 3 h after ingestion of paraquat and all patients received direct haemoperfusion therapy. Five out of 21 patients survived and 16 patients died within 28 days. Plasma paraquat concentrations in non-survivors (5740 +/- 380 microg l(-1)) were not significantly different from those in survivors ( 5920 +/- 280 microg l(-1)) before treatment. Haemoperfusion reduced these concentrations in both non-survivors (120 +/- 7 microg l(-1)) as well as survivors (136 +/- 9 microg l(-1)) on day 5. Serum concentrations of type IV collagen and TIMP-1 in survivors showed little change between day 1 (type IV collagen, 90.4 +/- 3.6 ng ml(-1); TIMP-1, 172.2 +/- 7.0 ng ml(-1)) and day 5 (type IV collagen, 92.6 +/- 4.2 ng ml(-1); TIMP-1, 174.2 +/- 7.2 ng ml(-1)). In contrast, these concentrations in non-survivors on day 5 (type IV collagen, 143.6 +/- 7.8 mg ml(-1); TIMP-1, 246.8 +/- 13.6 ng ml(-1)) were significantly higher than those on day 1 (type IV collagen, 88.4 +/- 4.2 ng ml(-1), P < 0.01; TIMP-1, 170.6 +/- 9.2 ng ml(-1), P < 0.05). These data suggest that serum concentrations of type IV collagen and TIMP-1 may be useful indicators for the development of respiratory failure in patients with paraquat poisoning.
authors.affiliation: Department of Medicine, Misato Junshin Hospital, Saitama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: C
authors.initials: C
authors.lastname: Ushiyama
authors.forename: N
authors.initials: N
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: I
authors.initials: I
authors.lastname: Ebihara
authors.forename: M
authors.initials: M
authors.lastname: Suzaki
authors.forename: H
authors.initials: H
authors.lastname: Koide
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D024141:Collagen Type IV / Q000097:blood*; D017809:Fatal Outcome; D005260:Female; D006464:Hemoperfusion; D006540:Herbicides / Q000506:poisoning*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010269:Paraquat / Q000506:poisoning*; D011041:Poisoning / Q000628:therapy; D011379:Prognosis; D012131:Respiratory Insufficiency / Q000175:diagnosis / Q000209:etiology*; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D024141:Collagen Type IV; D006540:Herbicides; D019715:Tissue Inhibitor of Metalloproteinase-1; D010269:Paraquat