title: Blood contamination and dentin bonding--effect of anticoagulant in laboratory studies.
abstract: OBJECTIVE
  To investigate the influence of heparinized blood vs. native blood on the marginal adaptation of dentin bonded composite resin restorations.
  
  
  METHODS
  Cylindrical dentin cavities were prepared in 40 extracted human incisors. After acid-etching four groups (n=10) were contaminated with either fresh capillary blood (FCB), heparinized venous blood (HPB), saline (SAL) or heparinized saline (HPS) and bonded and filled with Scotchbond 1/Z 100. After water storage for 21 days and thermocycling (2000 x, 5-55 degrees C), replicas were produced for evaluation of marginal adaptation by means of quantitative marginal analysis in the SEM. Statistical analysis was performed with the Kruskal-Wallis-test and the Bonferroni correction for multiple comparisons at p<0.05.
  
  
  RESULTS
  There were no differences between groups SAL, HPS and HPB. Contamination with fresh capillary blood resulted in significantly higher amounts of marginal openings compared to all other groups.
  
  
  CONCLUSIONS
  Freshly drawn blood has to be used for blood contamination experiments in laboratory studies.
authors.affiliation: Department of Periodontology and Synoptic Dentistry, Humboldt-University, Charité, Berlin, Germany. tommi@tomsoft.com
authors.forename: Thomas
authors.initials: T
authors.lastname: Dietrich
authors.forename: Martin L J
authors.initials: ML
authors.lastname: Kraemer
authors.forename: Jean Francois
authors.initials: JF
authors.lastname: Roulet
mesh_terms: D000134:Acid Etching, Dental; D000925:Anticoagulants / Q000737:chemistry*; D001769:Blood; D003188:Composite Resins / Q000737:chemistry*; D001840:Dental Bonding; D018772:Dental Marginal Adaptation; D003775:Dental Polishing; D003793:Dental Restoration, Permanent; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry; D006801:Humans; D007754:Laboratories, Dental; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry; D012965:Sodium Chloride; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013499:Surface Properties; D013816:Thermodynamics; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D014867:Water; D012965:Sodium Chloride; D006493:Heparin; C041330:Scotchbond