title: A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules.
abstract: Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.
authors.affiliation: Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5G 1L6.
authors.forename: Amy Hin Yan
authors.initials: AH
authors.lastname: Tong
authors.forename: Becky
authors.initials: B
authors.lastname: Drees
authors.forename: Giuliano
authors.initials: G
authors.lastname: Nardelli
authors.forename: Gary D
authors.initials: GD
authors.lastname: Bader
authors.forename: Barbara
authors.initials: B
authors.lastname: Brannetti
authors.forename: Luisa
authors.initials: L
authors.lastname: Castagnoli
authors.forename: Marie
authors.initials: M
authors.lastname: Evangelista
authors.forename: Silvia
authors.initials: S
authors.lastname: Ferracuti
authors.forename: Bryce
authors.initials: B
authors.lastname: Nelson
authors.forename: Serena
authors.initials: S
authors.lastname: Paoluzi
authors.forename: Michele
authors.initials: M
authors.lastname: Quondam
authors.forename: Adriana
authors.initials: A
authors.lastname: Zucconi
authors.forename: Christopher W V
authors.initials: CW
authors.lastname: Hogue
authors.forename: Stanley
authors.initials: S
authors.lastname: Fields
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
authors.forename: Gianni
authors.initials: G
authors.lastname: Cesareni
mesh_terms: D000465:Algorithms; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D019295:Computational Biology; D016384:Consensus Sequence; D003598:Cytoskeletal Proteins; D030541:Databases, Genetic; D030562:Databases, Protein; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020543:Proteome; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012984:Software; D020798:Two-Hybrid System Techniques; D051316:Wiskott-Aldrich Syndrome Protein; D018909:src Homology Domains
chemical_list: D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; C480361:LAS17 protein, S cerevisiae; D008024:Ligands; D019151:Peptide Library; D010455:Peptides; D011506:Proteins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D051316:Wiskott-Aldrich Syndrome Protein