Dynamic insertion-deletion of introns in deuterostome EF-1alpha genes.
To test the validity of intron-exon structure as a phylogenetic marker, the intron-exon structure of EF-1alpha genes was investigated for starfish, acornworms, ascidians, larvaceans, and amphioxus and compared with that of vertebrates. Of the 11 distinct intron insertion sites found within the coding regions of the deuterostome EF-1alpha genes, 7 are shared by several taxa, while the remainder are unique to certain taxa. Examination of the shared introns of the deuterostome EF-1alpha gene revealed that independent intron loss or intron insertion must have occurred in separate lineages of the deuterostome taxa. Maximum parsimony analysis of the intron-exon data matrix recovered five parsimonious trees (consistency index = 0.867). From this result, we concluded that the intron-exon structure of deuterostome EF-1alpha has evolved more dynamically than previously thought, rendering it unsuitable as a phylogenetic marker. We also reconstructed an evolutionary history of intron insertion-deletion events on the deuterostome phylogeny, based on several molecular phylogenetic studies. These analyses revealed that the deuterostome EF-1alpha gene has lost individual introns more frequently than all introns simultaneously.
Wada
Hiroshi
H
Seto Marine Biological Laboratory, Graduate School of Sciences, Kyoto University, 459 Shirahama, Wakayama, 649-2211, Japan. hwada@seto.kyoto-u.ac.jp
Kobayashi
Mari
M
Sato
Riki
R
Satoh
Nori
N
Miyasaka
Hitoshi
H
Shirayama
Yoshihisa
Y
D000595:Amino Acid Sequence; D000818:Animals; D002816:Chordata, Nonvertebrate / Q000235:genetics; D002938:Ciona intestinalis / Q000235:genetics; D004447:Echinodermata / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons; D017353:Gene Deletion; D007438:Introns; D007448:Invertebrates / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014561:Urochordata / Q000235:genetics; D014714:Vertebrates / Q000145:classification / Q000235:genetics
D020648:Peptide Elongation Factor 1