Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish.
The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilize eggs. It has been used to: monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage; and to optimise conditions for fertilisation. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.
Kime
D E
DE
Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK. d.kime@sheffield.ac.uk
Van Look
K J
KJ
McAllister
B G
BG
Huyskens
G
G
Rurangwa
E
E
Ollevier
F
F
D000818:Animals; D015925:Cryopreservation; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D005399:Fishes / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D019216:Metals, Heavy / Q000633:toxicity; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity
D019216:Metals, Heavy; D014874:Water Pollutants, Chemical