title: Post-natal ontogeny of stanniocalcin gene expression in rodent kidney and regulation by dietary calcium and phosphate.
abstract: BACKGROUND
  Stanniocalcin (STC) is a polypeptide hormone first discovered in fish and more recently in mammals. In mammals, STC is produced in many tissues and does not normally circulate in the blood. In kidney and gut, STC regulates phosphate fluxes across the transporting epithelia, whereas in brain it protects neurons against cerebral ischemia and promotes neuronal cell differentiation. The gene is highly expressed in ovary and dramatically up-regulated during pregnancy and nursing. Gene expression also is high during mammalian embryogenesis, particularly in kidney where the hormone signals between epithelial and mesenchymal cells during nephrogenesis.
  
  
  METHODS
  This study examined the patterns of STC gene expression and protein distribution in the mouse kidney over the course of post-natal development. Further, because STC is a regulator of renal phosphate transport, we also examined the effects of changing levels of dietary calcium and phosphate on renal levels of STC gene expression in adult rats.
  
  
  RESULTS
  STC mRNA levels in the neonate kidney were found to be tenfold higher than adults. Isotopic in situ hybridization of neonate kidneys revealed that most, if not all, STC mRNA was confined to collecting duct (CD) cells, as is the case in adults. STC protein on the other hand was found in proximal tubule, thick ascending limb and distal tubules in addition to CD cells. This suggests that, as in adults, the more proximal nephron segments in neonates are targeted by CD-derived STC and sequester large amounts of hormone. The addition of 1% calcium gluconate to the drinking water significantly reduced STC mRNA levels in inner medullary CD cells of both males and females, but not those in the cortex and outer medulla. Placing animals on low phosphate diets also reduced STC mRNA levels, but uniquely in outer medullary and cortical CD cells, whereas a high phosphate diet increased transcript levels in the same regions.
  
  
  CONCLUSIONS
  These findings suggest that STC may be of unique importance to neonates. They also suggest that changes in dietary calcium and phosphate can alter renal levels of STC gene expression, but that these effects vary between the early and late segments of the collecting duct.
authors.affiliation: Department of Physiology, Faculty of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Deol
authors.forename: S E
authors.initials: SE
authors.lastname: Stasko
authors.forename: P
authors.initials: P
authors.lastname: De Niu
authors.forename: K A
authors.initials: KA
authors.lastname: James
authors.forename: G F
authors.initials: GF
authors.lastname: Wagner
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000502:physiology*; D002136:Calcium, Dietary / Q000494:pharmacology*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D006023:Glycoproteins / Q000235:genetics*; D006728:Hormones / Q000235:genetics*; D007668:Kidney / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D010710:Phosphates / Q000008:administration & dosage* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002136:Calcium, Dietary; D006023:Glycoproteins; D006728:Hormones; D010710:Phosphates; D012333:RNA, Messenger; C051795:teleocalcin