Histogenesis of hippocampus and neocortex isolated from postnatal rats in organotypic roller tube cultures of floating brain sections.
Floating sections of the hippocampus and neocortex isolated from 6-10-day-old rats retained their spatial cell organization after 2-week roller-tube culturing. Cell structure in CA1, Ca2, and CA3 fields, polymorphic layer of the fascia dentata and its medial and lateral limbs were revealed in sections of the dorsal hippocampus. In neocortical sections, cortical neurons and subcortical structure were preserved, however, some cortical fragments changed their configuration and formed spherical structures, where cortical neurons were located in the external layer without forming typical of neocortex 6-layer structure.
Aleksandrova
O P
OP
Laboratory of Experimental Neurocytology, Brain Research Institute, Russian Academy of Medical Sciences.
Lyzhin
A A
AA
Viktorov
I V
IV
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000473:pathology; D018891:Dentate Gyrus / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D019579:Neocortex / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques / Q000379:methods*; D051381:Rats; D013997:Time Factors