title: Effect of acid resistance of Escherichia coli O157:H7 on efficacy of buffered lactic acid to decontaminate chilled beef tissue and effect of modified atmosphere packaging on survival of Escherichia coli O157:H7 on red meat.
abstract: The present study examined the effect of pH-independent acid resistance of Escherichia coli O157:H7 on efficacy of buffered lactic acid to decontaminate chilled beef tissue. A varied level of acid resistance was observed among the 14 strains tested. Eight strains were categorized as acid resistant, four strains as acid sensitive, and two strains demonstrated acid-inducible acid resistance. The survival of an acid-resistant (II/45/4) and acid-sensitive (IX/8/16) E. coli O157:H7 strain on chilled beef tissue treated with 1 and 2% buffered lactic acid, sterile water, or no treatment (control) was followed. A gradual reduction of E. coli O157:H7 was noticed during the 10 days of storage at 4 degrees C for each of the treatments. Decontamination with 1 and 2% buffered lactic acid did not appreciably affect the pathogen. Differences in the pH-independent acid resistance of the strains had no effect on the efficacy of decontamination. The effect of modified atmosphere packaging (MAP) on survival of E. coli O157:H7 in red meat was also studied. MAP (40% CO2/60% N2) or vacuum did not significantly influence survival of E. coli O157:H7 on inoculated sliced beef (retail cuts) meat compared to packing in air. The relative small outgrowth of lactic acid bacteria during storage under vacuum for 28 days did not affect survival of E. coli O157:H7. Neither lactic acid decontamination nor vacuum or MAP packaging could enhance reduction of E. coli O157:H7 on beef, thus underlining the need for preventive measures to control the public health risk of E. coli O157:H7.
authors.affiliation: Department of Food Technology and Nutrition, University of Gent, Belgium. mieke.uyttendaele@rug.ac.be
authors.forename: M
authors.initials: M
authors.lastname: Uyttendaele
authors.forename: E
authors.initials: E
authors.lastname: Jozwik
authors.forename: A
authors.initials: A
authors.lastname: Tutenel
authors.forename: L
authors.initials: L
authors.lastname: De Zutter
authors.forename: J
authors.initials: J
authors.lastname: Uradzinski
authors.forename: D
authors.initials: D
authors.lastname: Pierard
authors.forename: J
authors.initials: J
authors.lastname: Debevere
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004203:Disinfection / Q000379:methods*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000494:pharmacology*; D008460:Meat / Q000382:microbiology*; D013696:Temperature; D013997:Time Factors; D016896:Treatment Outcome; D014618:Vacuum
chemical_list: D019344:Lactic Acid