title: Crystallization of a large single crystal of a B-DNA decamer for a neutron diffraction experiment by the phase-diagram technique.
abstract: Crystallization of a large single crystal of a B-DNA decamer, d(CCATTAATGG), for a neutron-diffraction experiment has been accomplished by an analysis of its solubility phase diagram and a large single crystal was successfully crystallized at around the minimum solubility point of the oligonucleotide: 30%(v/v) MPD, 100 mM MgCl(2) pD 6.6 using 0.4 ml D(2)O solutions of the DNA (sample concentration 1.5 mM). It is confirmed that the resulting crystal (dimensions: 1.7 x 1.3 x 0.6 mm) diffracts sufficiently well for neutron data collection.
authors.affiliation: Advanced Science Research Center, Japan Atomic Energy Research Institute, Tokai-mura, Ibaraki-ken, 319-1195, Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Arai
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Chatake
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Minezaki
authors.forename: Nobuo
authors.initials: N
authors.lastname: Niimura
mesh_terms: D001483:Base Sequence; D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D009502:Neutrons; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA