Induced transcriptional expression of calcium-binding protein S100A1 and S100A10 genes in human renal cell carcinoma.
Expression of 16 S100 family calcium-binding protein genes was evaluated by PCR in ten human tissue cDNA libraries. Six to 12 S100 genes were expressed in a tissue-specific manner. Then, the expression in the surgically resected renal cell carcinoma (RCC) and a cultured RCC cell line was studied by RT-PCR. Although eight to nine S100 genes were transcribed in the normal kidney library and non-cancerous part of resected kidney tumors, S100A1 and S100A10 genes were not expressed. However, these genes were newly expressed in the RCC lesions (n=7) and the RCC cell line, indicating that expression of S100A1 and S100A10 genes is accompanied by RCC.
Teratani
Takumi
T
Laboratory of Host Defenses, Graduate School of Health Science, University of Shizuoka, Yada, 422-8526, Shizuoka, Japan.
Watanabe
Takumi
T
Kuwahara
Fuminari
F
Kumagai
Hiromichi
H
Kobayashi
Shuzou
S
Aoki
Utaka
U
Ishikawa
Akira
A
Arai
Kazumori
K
Nozawa
Ryushi
R
D017306:Annexin A2; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D017931:DNA Primers; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009418:S100 Proteins; D014158:Transcription, Genetic; D000099248:S100 Calcium Binding Protein A10
D017306:Annexin A2; D002135:Calcium-Binding Proteins; D017931:DNA Primers; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C110294:S100A1 protein