title: Studies on Bacillus stearothermophilus. Part 1. Transformation of progesterone to a new metabolite 9,10-seco-4-pregnene-3,9,20-trione.
abstract: When Bacillus stearothermophilus, a thermophilic bacterium isolated from the Kuwaiti desert, was incubated with exogenous progesterone for 24 h, three monohydroxylated metabolites were produced. 20alpha-Hydroxyprogesterone was the major metabolite produced in 60.8 relative percentage yield. The other two monohydroxylated metabolites were identified as 6beta-hydroxyprogesterone and the rare 6alpha-hydroxyprogesterone in 21.0 and 13.6 relative percentage yields, respectively. A new metabolite 9,10-seco-4-pregnene-3,9,20-trione was isolated in 3.7 relative percentage yield. All metabolites were purified by preparative TLC and HPLC followed by their identification using 1H, 13C NMR and other spectroscopic data.
authors.affiliation: Department of Biological Sciences, Biochemistry Program, Faculty of Science, Kuwait University, P.O. Box 5969, 13060, Safat, Kuwait. biosc@kuc01.kuniv.edu.kw
authors.forename: S
authors.initials: S
authors.lastname: Al-Awadi
authors.forename: M
authors.initials: M
authors.lastname: Afzal
authors.forename: S
authors.initials: S
authors.lastname: Oommen
mesh_terms: D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003889:Desert Climate; D001411:Geobacillus stearothermophilus / Q000302:isolation & purification / Q000378:metabolism*; D007730:Kuwait; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D011283:Pregnenes / Q000737:chemistry / Q000378:metabolism*; D011374:Progesterone / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: C447029:9,10-seco-4-pregnene-3,9,20-trione; D011283:Pregnenes; D011374:Progesterone