title: Phosphatidylglycerophosphate synthases from Arabidopsis thaliana.
abstract: Two Arabidopsis thaliana genes were shown to encode phosphatidylglycerophosphate synthases (PGPS) of 25.4 and 32.2 kDa, respectively. Apart from their N-terminal regions, the two proteins exhibit high sequence similarity. Functional expression studies in yeast provided evidence that the 25.4 kDa protein is a microsomal PGPS while the 32.2 kDa protein represents a preprotein which can be imported into yeast mitochondria and processed to a mature PGPS. The two isozymes were solubilized and purified as fusion proteins carrying a His tag at their C-terminus. Enzyme assays with both membrane fractions and purified enzyme fractions revealed that the two A. thaliana isozymes have similar properties but differ in their CDP-diacylglycerol species specificity.
authors.affiliation: RWTH Aachen, Institut für Biologie I, Spezielle Botanik, Worringer Weg 1, 52056, Aachen, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Frentzen
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D003567:Cytidine Diphosphate Diglycerides / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D003567:Cytidine Diphosphate Diglycerides; D007527:Isoenzymes; D011994:Recombinant Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase