title: Bovine neosporosis: immunoblot improves foetal serology.
abstract: The purpose of the study is the comparative evaluation of the immunofluorescent antibody test (IFAT) and an immunoblot (IB) test for the examination of foetal fluids for specific antibodies against Neospora caninum. Peritoneal and pleural fluids as well as abomasal contents were analysed. The results of the serological examinations were compared to those obtained by histological, immunohistochemical, and PCR analysis of foetal tissues as well as to the results of maternal serological examinations. Fluids were used undiluted in the IB and reactions against six immunodominant antigens were recorded. When the recognition of at least two immunodominant antigens was regarded as positive, the agreement of the IB with other diagnostic methods was good to moderate as characterised by kappa-values of 0.76 (histology/immunohistochemistry), 0.69 (maternal serology) and 0.54 (PCR on foetal tissues). The IB results agreed better with the results of the other diagnostic methods than those of the IFAT. The higher relative sensitivity of the IB was regarded as the main reason for the better agreement. However, also the specificity of the IB was superior to that of the IFAT in relation to histology/immunohistochemistry, maternal serology and PCR.
authors.affiliation: Staatliches Veterinäruntersuchungsamt, Zur Taubeneiche 10-12, D-59821, Arnsberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Söndgen
authors.forename: M
authors.initials: M
authors.lastname: Peters
authors.forename: A
authors.initials: A
authors.lastname: Bärwald
authors.forename: R
authors.initials: R
authors.lastname: Wurm
authors.forename: F
authors.initials: F
authors.lastname: Holling
authors.forename: F J
authors.initials: FJ
authors.lastname: Conraths
authors.forename: G
authors.initials: G
authors.lastname: Schares
mesh_terms: D000018:Abomasum / Q000196:embryology / Q000469:parasitology; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D001202:Ascitic Fluid / Q000196:embryology / Q000469:parasitology; D001921:Brain / Q000196:embryology / Q000469:parasitology / Q000473:pathology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000196:embryology / Q000469:parasitology; D003048:Coccidiosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005333:Fetus / Q000469:parasitology*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary; D015151:Immunoblotting / Q000379:methods / Q000662:veterinary*; D007150:Immunohistochemistry; D018688:Neospora / Q000276:immunology*; D010994:Pleura / Q000196:embryology / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan