Assessing the role of calcium-induced calcium release in short-term presynaptic plasticity at excitatory central synapses.
Recent evidence suggests that internal calcium stores and calcium-induced calcium release (CICR) provide an important source of calcium that drives short-term presynaptic plasticity at central synapses. Here we tested for the involvement of CICR in short-term presynaptic plasticity at six excitatory synapses in acute rat hippocampal and cerebellar brain slices. Depletion of internal calcium stores with thapsigargin and prevention of CICR with ryanodine have no effect on paired-pulse facilitation, delayed release of neurotransmitter, or calcium-dependent recovery from depression. Fluorometric calcium measurements also show that these drugs have no effect on the residual calcium signal that underlies these forms of short-term presynaptic plasticity. Finally, although caffeine causes CICR in Purkinje cell bodies and dendrites, it does not elicit CICR in parallel fiber inputs to these cells. Taken together, these results indicate that for the excitatory synapses studied here, internal calcium stores and CICR do not contribute to short-term presynaptic plasticity on the milliseconds-to-seconds time scale. Instead, this plasticity is driven by the residual calcium signal arising from calcium entry through voltage-gated calcium channels.
Carter
Adam G
AG
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Vogt
Kaspar E
KE
Foster
Kelly A
KA
Regehr
Wade G
WG
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000378:metabolism; D011689:Purkinje Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012433:Ryanodine / Q000494:pharmacology; D013569:Synapses / Q000378:metabolism*; D019284:Thapsigargin / Q000494:pharmacology
D015220:Calcium Channels; D002614:Chelating Agents; D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D012433:Ryanodine; D002110:Caffeine; D019284:Thapsigargin; D002118:Calcium