title: Conformational isomers of insect odorant-binding proteins.
abstract: We have identified and cloned the cDNAs encoding odorant-binding proteins (OBPs) from the large black chafer, Holotrichia parallela, and the yellowish elongate chafer, Heptophylla picea. Each species possess two OBPs, the proteins migrating faster in native gels (OBP1) showed high amino acid identity (>88%) to previously identified pheromone-binding proteins (PBPs) from scarab beetles. HparOBP1 and HpicOBP1 have 116 amino acids and six highly conserved cysteine residues. In contrast to OBP1 that gave a single band, both HparOBP2 and HpicOBP2 separated each into two bands in native gels (15%). The N-terminal amino acid sequences for the two bands from each species were indistinguishable, and they had the same molecular masses. Although we sequenced several clones from each species, they all encode only one protein for each species, indicating they are different conformational isomers of the same protein. HparOBP2 and HpicOBP2 have 133 amino acids and cysteine residues are conserved in proteins of the same family.
authors.affiliation: Laboratory of Chemical Prospecting, National Institute of Agriobiological Sciences, 1-2 Ohwashi, Tsukuba, 305-8634, Japan.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Deyu
authors.forename: Walter Soares
authors.initials: WS
authors.lastname: Leal
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D003001:Cloning, Molecular; D001517:Coleoptera; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007313:Insecta; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D018035:Receptors, Odorant / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D018035:Receptors, Odorant; C045648:odorant-binding protein