Teaching old indicators new tricks.
Most synthetic sensors are designed with covalent attachment between a receptor and a reporter moiety. In this report, we describe the current progress of our use of noncovalently attached indicators to signal binding of analytes. With these systems, analyte binding leads to indicator displacement from the binding cavity, which in turn yields an optical signal modulation. We include previous examples, the strategies involved in our development, and the advantages as well as disadvantages of this method. Finally, our latest research in this field is briefly presented.
Wiskur
S L
SL
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
Ait-Haddou
H
H
Lavigne
J J
JJ
Anslyn
E V
EV
D001665:Binding Sites; D015374:Biosensing Techniques; D019343:Citric Acid / Q000032:analysis / Q000737:chemistry; D019298:Glucose-6-Phosphate / Q000032:analysis / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry*; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis / Q000737:chemistry; D009566:Nitrates / Q000032:analysis / Q000737:chemistry; D013644:Tartrates / Q000032:analysis / Q000737:chemistry
D007202:Indicators and Reagents; D009566:Nitrates; D013644:Tartrates; D019343:Citric Acid; D019298:Glucose-6-Phosphate; D015544:Inositol 1,4,5-Trisphosphate; C029768:tartaric acid