title: Bone and bone-marrow interactions: haematological activity of osteoblastic growth peptide (OGP)-derived carboxy-terminal pentapeptide. Mobilizing properties on white blood cells and peripheral blood stem cells in mice.
abstract: Osteogenic growth peptide (OGP) increases blood and bone marrow cellularity in mice, and enhances engraftment of bone marrow transplant. Carboxy-terminal pentapeptide (OGP10-14) holds several properties of full-length polypeptide. We evaluated whether synthetic OGP-derived pentapeptide (sOGP10-14) has some activity on peripheral blood cell recovery after cyclophosphamide-induced aplasia, and on stem cell mobilization. Peripheral blood stem cell (PBSC) mobilization was evaluated by administering granulocyte-colony stimulating factor (G-CSF) or sOGP10-14 after cyclophosphamide (CTX) injection. Haematological parameters and CD34/Sca-1 positive cells were sequentially evaluated. Colony-forming tests were performed in bone marrow cells from CTX-, G-CSF- and sOGP10-14-treated mice. sOGP10-14 was able to enhance band cells and monocyte recovery after cyclophosphamide administration. White blood cell (WBC) counts reached the maximum peak by day +10 but, on day +7, a significant recovery was already detected in sOGP10-14 treated mice. On day +10 the WBC increase in sOGP10-14-treated mice was comparable to that found in G-CSF treated ones. Moreover, CD34/Sca-1 positive early precursors were significantly mobilized by sOGP10-14 compared to the control group. In sOGP10-14-treated mice, the colony-forming unit-granulocyte-macrophage-megakaryocyte (GEMM-CFU) and burst-forming unit-erythroid (BFU-E) were significantly increased in bone marrow cells in comparison to mice treated with CTX only. These results suggest a central role of sOGP10-14 in bone and bone marrow interaction, and a possible role of sOGP10-14 as a mobilizing agent.
authors.affiliation: Department of Oncology, Transplants and Advanced Technologies in Medicine, Hematology Division, University of Pisa, Via Roma 67, 56100, Pisa, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Fazzi
authors.forename: Rossana
authors.initials: R
authors.lastname: Testi
authors.forename: Silvia
authors.initials: S
authors.lastname: Trasciatti
authors.forename: Sara
authors.initials: S
authors.lastname: Galimberti
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosini
authors.forename: Francesca
authors.initials: F
authors.lastname: Piras
authors.forename: Gianluca
authors.initials: G
authors.lastname: L'Abbate
authors.forename: Angela
authors.initials: A
authors.lastname: Conte
authors.forename: Mario
authors.initials: M
authors.lastname: Petrini
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D001842:Bone and Bones / Q000378:metabolism*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000633:toxicity; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D012010:Red-Cell Aplasia, Pure / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D018952:Antigens, CD34; D018906:Antineoplastic Agents, Alkylating; D006133:Growth Substances; D006657:Histones; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C074533:osteogenic growth peptide; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003520:Cyclophosphamide