Identification of peptide sequences specific for serum antibodies from human papillomavirus-infected patients using phage display libraries.
Three random phage display peptide libraries were screened with sera from human papillomavirus (HPV)-infected patients to characterize the specificities of antibodies present in patients' sera and to identify molecules that correspond to or mimic natural epitopes; 141 phage clones were randomly selected in three rounds of bioselection and their binding properties were analyzed in ELISA using sera from 36 patients with confirmed HPV 16 infection and 24 healthy control female blood donors. Sixteen of 36 (44%) patients' sera reacted with at least 1 phage clone, and only 2 of 24 female donors' sera showed positive reaction with 1 of the selected clones. We conclude that the combination of various disease-specific epitopes generated by screening of phage display peptide libraries may potentially lead to a multicomponent diagnostic assay for the early detection of HPV infection and precancerous cervical lesions, making possible the prevention of one of the most common cancers in women.
Santamaria
H
H
Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de México, Ciudad Universitaria, C.P. 04510, Mexico, DF, Mexico.
Manoutcharian
K
K
Rocha
L
L
Gonzalez
E
E
Acero
G
G
Govezensky
T
T
Uribe
L I
LI
Olguin
A
A
Paniagua
J
J
Gevorkian
G
G
D000914:Antibodies, Viral / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology*; D030361:Papillomavirus Infections / Q000097:blood / Q000276:immunology*; D019151:Peptide Library; D014412:Tumor Virus Infections / Q000097:blood / Q000276:immunology*
D000914:Antibodies, Viral; D016056:Immunodominant Epitopes; D019151:Peptide Library