A new and simple procedure for the evaluation of the association of surfactants to proteins.
A new and simple method useful for the evaluation of the association of surfactants to proteins is proposed. The method is based on an analysis of the effect promoted by surfactant addition upon the fluorescence intensity of the intrinsic tryptophan chromophore and its dependence with protein concentration. The proposed methodology is applied to quantify the binding of an anionic (sodium dodecylsulfate), a zwitterionic (N-hexadecyl-N,N'-dimethyl-3-ammonio-1-propane-sulfonate) and a neutral (Triton X-100, reduced) surfactant to bovine serum albumin (BSA).
Lissi
Eduardo
E
Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40-Correo 33, Santiago, Chile. elissi@lauca.usach.cl
Abuin
Elsa
E
Lanio
María E
ME
Alvarez
Carlos
C
D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D017830:Octoxynol / Q000737:chemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry
C094641:N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; D011506:Proteins; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D014364:Tryptophan; D017830:Octoxynol