Postneurosurgical meningitis due to Proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class A beta-lactamase HugA and its LysR-type regulatory protein HugR.
We report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible Proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. The isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single beta-lactamase named HugA with an isoelectric point of 6.7. The ceftriaxone-resistant isolate hyperproduced the beta-lactamase (increase in the level of production, about 90-fold). The sequences of the hugA beta-lactamase gene and its regulator, hugR, were identical in both P. penneri strains and had 85.96% homology with those of Proteus vulgaris. The HugA beta-lactamase belongs to molecular class A, and the transcriptional regulator HugR belongs to the LysR family.
Liassine
Nadia
N
Central Laboratory of Bacteriology, University Hospital, Geneva, Switzerland. nliassine@unilabs.ch
Madec
Stéphanie
S
Ninet
Béatrice
B
Metral
Catherine
C
Fouchereau-Peron
Martine
M
Labia
Roger
R
Auckenthaler
Raymond
R
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002443:Ceftriaxone / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D004351:Drug Resistance; D006801:Humans; D016920:Meningitis, Bacterial / Q000382:microbiology*; D008969:Molecular Sequence Data; D011183:Postoperative Complications / Q000382:microbiology*; D011511:Proteus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
D004269:DNA, Bacterial; D002443:Ceftriaxone; D001618:beta-Lactamases