Transfection-mediated cell synchronization: acceleration of G1-S phase transition by gamma irradiation.
We have previously provided evidence that the uptake of DNA into cells is cell cycle specific following transfection. We show here that, immediately after transfection, successfully transfected cells are greatly enriched for cells in early G1 or G0 phase and that, upon removal of the DNA precipitates, cells progress through G1 and enter S phase in a synchronous fashion. We also demonstrate that this approach can be utilized in meaningful cell-cycle experiments, and we show that gamma irradiation accelerates the G1-S phase transition in a cell line with a functionally inactive p53 protein.
Jung
E J
EJ
Tulane Health Sciences Center, New Orleans, LA 70112, USA.
Flemington
E K
EK
D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D016193:G1 Phase / Q000528:radiation effects*; D005720:Gamma Rays; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D016196:S Phase / Q000528:radiation effects*; D014162:Transfection; D025521:Tumor Suppressor Proteins
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases