Effect of epristeride on expression of TGF-beta receptor in rat prostatic epithelial cells in vitro.
OBJECTIVE
To assess the effect of epristeride on the expression of transforming growth factor beta type II receptor (TbetaR II) in rat prostatic epithelial cells in vitro.


METHODS
RT-PCR and Western blot were used to quantitatively detect the mRNA and protein expressions of TbetaR II in rat prostatic epithelial cells treated or untreated with epristeride. Immunocytochemical staining method was used to qualitatively analyze the expression of TbetaR II protein.


RESULTS
After treatment with epristeride 180 or 360 nmol/L, TbetaR II mRNA expression levels were 0.56 +/- 0.08 and 0.59 +/- 0.07, respectively, which were significantly up-regulated compared with control cells (0.38 +/- 0.04, P < 0.05); expression level of TbetaR II protein were 3163 +/- 920 and 6769 +/- 1941, respectively, which were also markedly up-regulated compared with control cells (536 +/- 240, P < 0.05). Immunostaining showed weak positive reaction in control cells, while strong staining of TbetaR II was found in epristeride-treated cells.


CONCLUSIONS
Epristeride may up-regulate the expression of TbetaR II to induce apoptosis of prostatic epithelial cells.
Wu
S F
SF
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Sun
H Z
HZ
Tu
Z H
ZH
Wu
H Y
HY
D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D000730:Androstadienes; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; C072547:epristeride