An oxyanion-hole selective serine protease inhibitor in complex with trypsin.
p-amidinophenylmethylphosphinic acid (AMPA) was designed, synthesized and crystallized in complex with trypsin to study interactions with the oxyanion hole at the S1 site. In comparison to benzamidine, AMPA shows improved activity, which the crystal structure demonstrates to result from hydrogen bonds between the negatively charged phosphinic acid group and the catalytic residues at the oxyanion hole.
Cui
Jian
J
Center for Pharmaceutical Biotechnology, College of Pharmacy, University of Illinois at Chicago, 900 S. Ashland Avenue, M/C 870, Chicago, IL 60607-7173, USA.
Marankan
Fatima
F
Fu
Wentao
W
Crich
David
D
Mesecar
Andrew
A
Johnson
Michael E
ME
D000838:Anions; D018360:Crystallography, X-Ray; D006801:Humans; D010721:Phosphinic Acids / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*
D000838:Anions; D010721:Phosphinic Acids; D015842:Serine Proteinase Inhibitors; C448823:p-amidinophenylmethylphosphinic acid; D014357:Trypsin