Application of the chromatin immunoprecipitation method to identify in vivo protein-DNA associations in fission yeast.
The chromatin immunoprecipitation (ChIP) method provides an ideal tool for detecting direct or indirect interactions between proteins of interest and DNAs with known sequences. Here, we introduce the ChIP protocol used in our laboratory to identify in vivo protein-DNA association in the fission yeast Schizosaccharomyces pombe. The cytological and genetic merits of the fission yeast for studying control of the eukaryotic cell cycle and chromosome dynamics are reinforced by application of this ChIP method.
Takahashi
K
K
Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kyoto, Japan. takahash@kozo.biophys.kyoto-u.ac.jp
Saitoh
S
S
Yanagida
M
M
D000906:Antibodies / Q000378:metabolism; D002457:Cell Extracts / Q000737:chemistry; D002843:Chromatin / Q000302:isolation & purification* / Q000378:metabolism; D004271:DNA, Fungal / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000276:immunology / Q000302:isolation & purification*; D011233:Precipitin Tests / Q000379:methods*; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000276:immunology / Q000302:isolation & purification
D000906:Antibodies; D002457:Cell Extracts; D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins