Determination of phenoxyacid herbicides and their phenolic metabolites in surface and drinking water.
An evaluation was made of the feasibility of using reversed-phase liquid chromatography/tandem mass spectrometry with an electrospray interface (LC/ESI-MS/MS) to measure traces of phenoxyacid herbicides and their metabolites in surface and drinking water samples. The procedure involved passing 0.5 L of river and drinking water samples through a 0.5 g graphitized carbon black (GCB) extraction cartridge. Recovery was higher than 85% irrespective of the aqueous matrix in which the analytes were dissolved. A conventional 4.6-mm i.d. reversed-phase LC C-18 column operating with a mobile phase flow rate of 1 mL/min was used to chromatograph the analytes. A flow of 200 microL/min of the column effluent was diverted to the ESI source. The limits of detection (signal-to-noise ratio = 3) of the method for the pesticides considered in drinking and surface water samples are less than 0.1 ng/L for phenoxyacid herbicides, and about 5-10 ng/L for their metabolites (2,4-dichlorophenol and 4-chloro-2-methylphenol).
Marchese
Stefano
S
Laboratorio Chimico per la Sicurezza, Dipartimento di Chimica, Università La Sapienza di Roma, Piazzale Aldo Moro n degrees 5, P.O. Box 34, Posta 62, 00185 Roma, Italy. stefano.marchese@uniroma.1.it
Perret
Daniela
D
Gentili
Alessandra
A
D'Ascenzo
Guiseppe
G
Faberi
Angelo
A
D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002087:Butyrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D011392:Proline / Q000031:analogs & derivatives* / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D011786:Quality Control; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014867:Water / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis*
D002087:Butyrates; D006540:Herbicides; D010636:Phenols; D011725:Pyridines; D014873:Water Pollutants; D014867:Water; C062806:4,4-dicarboxy-5-pyridoxylproline; D015084:2,4-Dichlorophenoxyacetic Acid; D011392:Proline; D008456:2-Methyl-4-chlorophenoxyacetic Acid; C008418:2-methyl-4-chlorophenoxy gamma-butyric acid; C004764:4-(2,4-dichlorophenoxy)butyric acid