title: Immunity to placental malaria. III. Impairment of interleukin(IL)-12, not IL-18, and interferon-inducible protein-10 responses in the placental intervillous blood of human immunodeficiency virus/malaria-coinfected women.
abstract: Pregnant women are highly susceptible to malaria, and human immunodeficiency virus (HIV) infection increases this susceptibility. In our previous studies, placental malaria (PM), HIV infection, and HIV/PM coinfection were all associated with decreased interferon (IFN)-gamma production by maternal placental (intervillous) blood mononuclear cells (IVBMC). This study investigated whether in vitro production of the IFN-gamma regulatory cytokines interleukin (IL)-12 and IL-18 and the chemokine IFN-inducible protein (IP)-10 by IVBMC is altered in women who have been exposed to malaria and are infected with HIV. IL-12 production from IVBMC was significantly lower in HIV-positive women, regardless of PM status, in contrast to HIV-negative, PM-negative women. IL-18 and IP-10 production by IVBMC was reduced in HIV-positive, PM-negative women but elevated in HIV-positive, PM-positive women. These results reveal a substantial impairment of IL-12 production by IVBMC in HIV-positive women, implicating this cytokine as a potentially critical regulator of malaria antigen-specific IFN-gamma responses in HIV-infected and HIV/PM-coinfected women.
authors.affiliation: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Sujittra
authors.initials: S
authors.lastname: Chaisavaneeyakorn
authors.forename: Julie M
authors.initials: JM
authors.lastname: Moore
authors.forename: Juliana
authors.initials: J
authors.lastname: Otieno
authors.forename: Sansanee C
authors.initials: SC
authors.lastname: Chaiyaroj
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Perkins
authors.forename: Ya Ping
authors.initials: YP
authors.lastname: Shi
authors.forename: Bernard L
authors.initials: BL
authors.lastname: Nahlen
authors.forename: Altaf A
authors.initials: AA
authors.lastname: Lal
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Udhayakumar
mesh_terms: D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000097:blood*; D002824:Chorionic Villi / Q000098:blood supply / Q000276:immunology; D004198:Disease Susceptibility; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000453:epidemiology / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D020382:Interleukin-18 / Q000097:blood; D007378:Interleukins / Q000097:blood*; D007630:Kenya / Q000453:epidemiology; D016778:Malaria, Falciparum / Q000150:complications* / Q000453:epidemiology / Q000276:immunology; D010920:Placenta / Q000098:blood supply / Q000276:immunology*; D010922:Placenta Diseases / Q000150:complications* / Q000453:epidemiology / Q000276:immunology; D011247:Pregnancy
chemical_list: D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D020382:Interleukin-18; D007378:Interleukins; D018664:Interleukin-12; D007371:Interferon-gamma