title: Diagnosis of Oropouche virus infection by RT-nested-PCR.
abstract: Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.
authors.affiliation: Virus Research Unit, School of Medicine of Ribeirão Preto, University of São Paulo, Campus da USP, Ribeirão Preto, SP, Brazil.
authors.forename: Marcos Lázaro
authors.initials: ML
authors.lastname: Moreli
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Aquino
authors.forename: Ana Cecília R
authors.initials: AC
authors.lastname: Cruz
authors.forename: Luiz Tadeu M
authors.initials: LT
authors.lastname: Figueiredo
mesh_terms: D001483:Base Sequence; D002044:Bunyaviridae Infections / Q000175:diagnosis*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012840:Simbu virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral