Immobilized halogenophenylcarbamate derivatives of cellulose as novel stationary phases for enantioselective drug analysis.
Three different halogeno-phenylcarbamate derivatives of cellulose have been prepared and coated on silica gel. The coated materials have been immobilized and their chiral recognition ability as chiral stationary phase (CSP) has been evaluated with a set of reference racemates, including several drugs such as lormetazepam, glutethimide, and warfarin, using various mobile phase mixtures. The novel phases were found to exhibit unique enantioselective properties compared with more established polysaccharide-based CSPs. A good resolution of all racemates could be successfully achieved on at least one of the immobilized CSPs. Moreover, it has been pointed out that selectivity may considerably vary with the composition of the mobile phase.
Francotte
E
E
NOVARTIS Pharma AG, Research Department, Central Technologies, WKL-122.P.25, Postfach, CH-4002 Basel, Switzerland. eric.francotte@pharma.novartis.com
Huynh
D
D
D002219:Carbamates / Q000032:analysis / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006219:Halogens / Q000032:analysis / Q000737:chemistry; D013237:Stereoisomerism
D002219:Carbamates; D006219:Halogens; D002482:Cellulose; C059692:phenylcarbamic acid