title: Postneurosurgical meningitis due to Proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class A beta-lactamase HugA and its LysR-type regulatory protein HugR.
abstract: We report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible Proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. The isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single beta-lactamase named HugA with an isoelectric point of 6.7. The ceftriaxone-resistant isolate hyperproduced the beta-lactamase (increase in the level of production, about 90-fold). The sequences of the hugA beta-lactamase gene and its regulator, hugR, were identical in both P. penneri strains and had 85.96% homology with those of Proteus vulgaris. The HugA beta-lactamase belongs to molecular class A, and the transcriptional regulator HugR belongs to the LysR family.
authors.affiliation: Central Laboratory of Bacteriology, University Hospital, Geneva, Switzerland. nliassine@unilabs.ch
authors.forename: Nadia
authors.initials: N
authors.lastname: Liassine
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Madec
authors.forename: Béatrice
authors.initials: B
authors.lastname: Ninet
authors.forename: Catherine
authors.initials: C
authors.lastname: Metral
authors.forename: Martine
authors.initials: M
authors.lastname: Fouchereau-Peron
authors.forename: Roger
authors.initials: R
authors.lastname: Labia
authors.forename: Raymond
authors.initials: R
authors.lastname: Auckenthaler
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002443:Ceftriaxone / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D004351:Drug Resistance; D006801:Humans; D016920:Meningitis, Bacterial / Q000382:microbiology*; D008969:Molecular Sequence Data; D011183:Postoperative Complications / Q000382:microbiology*; D011511:Proteus / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D002443:Ceftriaxone; D001618:beta-Lactamases