Serum protein profiles of patients with pancreatic cancer and chronic pancreatitis: searching for a diagnostic protein pattern.
In this study, 13 sera from patients with pancreatic cancer, 9 from chronic pancreatitis and 10 from healthy subjects were analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The MALDI mass spectra revealed the presence of several low molecular weight peptides, among which some were detected only in the sera from both pathological conditions. On the other hand many peptides were observed only in control sera, and were absent in the sera from the two diseases. Therefore, MALDI analysis of the low molecular weight fraction (<10 000 Da) of sera from patients with pancreatic diseases enabled us to identify the presence of some disease-related signals and also some signals characteristic of normal subjects.
Valerio
A
A
Department of Clinical and Experimental Medicine, University of Padova, Italy. ana.valerio@unipd.it
Basso
D
D
Mazza
S
S
Baldo
G
G
Tiengo
A
A
Pedrazzoli
S
S
Seraglia
R
R
Plebani
M
M
D000368:Aged; D014408:Biomarkers, Tumor; D001798:Blood Proteins / Q000032:analysis*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis; D010195:Pancreatitis / Q000097:blood* / Q000175:diagnosis; D010446:Peptide Fragments / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D010446:Peptide Fragments