title: Monoclonal antibodies and human sera directed to the secreted glycoprotein G of herpes simplex virus type 2 recognize type-specific antigenic determinants.
abstract: Glycoprotein G-2 (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted amino-terminal portion (sgG-2) and to a cell-associated carboxy-terminal portion which is further O-glycosylated to constitute the mature gG-2 (mgG-2). In contrast to mgG-2, which is known to elicit a type-specific antibody response in the human host, information on the immunogenic properties of sgG-2 is lacking. Here the sgG-2 protein was purified on a heparin column and used for production of monoclonal antibodies (mAbs). Four anti-sgG-2 mAbs were mapped using a Pepscan technique and identified linear epitopes which localized to the carboxy-terminal part of the protein. One additional anti-sgG-2 mAb, recognizing a non-linear epitope, was reactive to three discrete peptide stretches where the most carboxy-terminally located stretch was constituted by the amino acids (320)RRAL(323). Although sgG-2 is rapidly secreted into the cell-culture medium after infection, the anti-sgG-2 mAbs identified substantial amounts of sgG-2 in the cytoplasm of HSV-2-infected cells. All of the anti-sgG-2 mAbs were HSV-2 specific showing no cross-reactivity to HSV-1 antigen or to HSV-1-infected cells. Similarly, sera from 50 HSV-2 isolation positive patients were all reactive to sgG-2 in an enzyme immunoassay whilst no reactivity was seen in 25 sera from HSV-1 isolation positive patients or in 25 serum samples from HSV-negative patients suggesting that sgG-2 is a novel antigen potentially suitable for type-discriminating serodiagnosis.
authors.affiliation: Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden1.
authors.forename: Jan-Åke
authors.initials: JÅ
authors.lastname: Liljeqvist
authors.affiliation: Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden1.
authors.forename: Edward
authors.initials: E
authors.lastname: Trybala
authors.affiliation: UPR9021 du CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France2.
authors.forename: Johan
authors.initials: J
authors.lastname: Hoebeke
authors.affiliation: Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden1.
authors.forename: Bo
authors.initials: B
authors.lastname: Svennerholm
authors.affiliation: Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden1.
authors.forename: Tomas
authors.initials: T
authors.lastname: Bergström
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005260:Female; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D008432:Mathematical Computing; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D014759:Viral Envelope Proteins; C044525:glycoprotein B, herpes simplex virus type 2