title: The coxsackievirus and adenovirus receptor is a transmembrane component of the tight junction.
abstract: The coxsackievirus and adenovirus receptor (CAR) mediates viral attachment and infection, but its physiologic functions have not been described. In nonpolarized cells, CAR localized to homotypic intercellular contacts, mediated homotypic cell aggregation, and recruited the tight junction protein ZO-1 to sites of cell-cell contact. In polarized epithelial cells, CAR and ZO-1 colocalized to tight junctions and could be coprecipitated from cell lysates. CAR expression led to reduced passage of macromolecules and ions across cell monolayers, and soluble CAR inhibited the formation of functional tight junctions. Virus entry into polarized epithelium required disruption of tight junctions. These results indicate that CAR is a component of the tight junction and of the functional barrier to paracellular solute movement. Sequestration of CAR in tight junctions may limit virus infection across epithelial surfaces.
authors.affiliation: Division of Immunologic and Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA. cohenc@email.chop.edu
authors.forename: C J
authors.initials: CJ
authors.lastname: Cohen
authors.forename: J T
authors.initials: JT
authors.lastname: Shieh
authors.forename: R J
authors.initials: RJ
authors.lastname: Pickles
authors.forename: T
authors.initials: T
authors.lastname: Okegawa
authors.forename: J T
authors.initials: JT
authors.lastname: Hsieh
authors.forename: J M
authors.initials: JM
authors.lastname: Bergelson
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D018920:Coculture Techniques; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D004285:Dogs; D006801:Humans; D008564:Membrane Potentials; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011233:Precipitin Tests; D011991:Receptors, Virus / Q000502:physiology*; D019108:Tight Junctions / Q000502:physiology*
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus