title: Expression of bone morphogenetic protein-5 gene during chick heart development: possible roles in valvuloseptal endocardial cushion formation.
abstract: The bone morphogenetic protein (BMP) family, comprising multifunctional peptide growth factors, regulates many developmental processes in a variety of tissues. We examined the spatiotemporal expression of BMP5 by in situ hybridization in chick embryonic hearts from stages 5 to 33. The BMP5 gene was first expressed in the endoderm underlying the precardiac mesoderm at stages 5 to 8. Thereafter, BMP5 expression was restricted to the myocardium of the atrioventricular (AV) canal and outflow tract (OT) regions, where the valvuloseptal endocardial cushion tissue is induced. These results suggest that BMP5 may play important roles not only in myocardial differentiation, but also in the formation and maintenance of endocardial cushion tissue.
authors.affiliation: Department of Anatomy, Saitama Medical School, Moroyama-machi, Iruma-gun, Saitama, Japan. toshiy@saitama-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yamagishi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakajima
authors.forename: S
authors.initials: S
authors.lastname: Nishimatsu
authors.forename: T
authors.initials: T
authors.lastname: Nohno
authors.forename: K
authors.initials: K
authors.lastname: Ando
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D055417:Bone Morphogenetic Protein 5; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D002642:Chick Embryo; D017931:DNA Primers / Q000737:chemistry; D005314:Embryonic and Fetal Development; D004699:Endocardium / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D006346:Heart Septum / Q000196:embryology*; D006351:Heart Valves / Q000196:embryology*; D017403:In Situ Hybridization; D009206:Myocardium / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D055417:Bone Morphogenetic Protein 5; D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D012333:RNA, Messenger