title: Femcoat, a novel eggshell protein in Drosophila: functional analysis by double stranded RNA interference.
abstract: In Drosophila oogenesis, follicle cells derived from somatic tissue surround the oocyte and play key roles in generating properly polarized oocytes. During the later steps of oogenesis, follicle cells are involved in secretion of proteins that make the eggshell, an essential protective layer for the oocyte. Although studies on the signaling processes to make polarized oocytes have been progressed very far, studies on the mechanisms for eggshell formation is not clear yet. To elucidate the underlying mechanism in eggshell formation, we used a differential display screen to isolate genes that are specifically expressed during the later stages of oogenesis, and isolated a novel gene, Femcoat. Femcoat encodes a putative chorion membrane protein that contains many highly charged residues and has a putative signal peptide. Femcoat is expressed specifically in the follicle cells with a punctate staining pattern typical of secreted proteins, and becomes cross-linked heavily at the final steps of oogenesis. To identify the developmental role of Femcoat in eggshell formation, we performed an inducible double stranded RNA mediated interference (dsRNAi) method to specifically reduce Femcoat expression during oogenesis in adult flies. Electron microscopy analysis of egg chambers from these flies showed defects in chorion formation. These pieces of evidence demonstrated that Femcoat is necessary for eggshell formation, especially during chorion synthesis. Our results demonstrate that inducible dsRNAi analysis can be effective in determining the developmental function of novel genes.
authors.affiliation: Department of Molecular Biology, School of Natural Sciences, Sejong University, Seoul, 143-747, South Korea.
authors.forename: Chun
authors.initials: C
authors.lastname: Kim
authors.forename: Kyulee
authors.initials: K
authors.lastname: Han
authors.forename: Juri
authors.initials: J
authors.lastname: Kim
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Yi
authors.forename: Changsoo
authors.initials: C
authors.lastname: Kim
authors.forename: Jeongbin
authors.initials: J
authors.lastname: Yim
authors.forename: Young-Joon
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jeongsil
authors.initials: J
authors.lastname: Kim-Ha
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002823:Chorion / Q000196:embryology; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics* / Q000502:physiology; D004527:Egg Proteins / Q000235:genetics* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000235:genetics / Q000502:physiology; D016372:RNA, Antisense / Q000235:genetics; D012330:RNA, Double-Stranded / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D004527:Egg Proteins; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; C453728:femcoat protein, Drosophila