The association between sporadic somatic parental aneuploidy and chromosomally abnormal placentae in habitual abortions.
To determine whether there is a correlation between the proportion of aneuploid cells in peripheral lymphocytes and the karyotype of the abortus in recurrent miscarriage. 40 couples with recurrent miscarriage and their abortuses were cytogenetically analyzed according to the analysis of 60 cells per proband. Women were divided into two groups according to the proportion of chromosomally abnormal cells in the abortus. Chromosomal analysis was performed using G-banding with trypsin-Giemsa in parental peripheral blood and in the abortus. 20 of the 40 abortuses had a chromosomally abberant karyotype. 65% of parents aborting an embryo with with an increased proportion of chromosomally aberrant cells, had more than 10% aneuploid cells in their peripheral lymphocytes. However, only 12.5% of couples aborting an embryo with chromosomally normal cells had increased rates of aneuploidy. An increased proportion of aneuploid cells was found in the lymphocytes of recurrently aborting couples who repeatedly abort chromosomally abnormal fetuses. Mitotic instability in the lymphocytes may indicate a predisposition to instability at meiosis leading to a chromosomal abberations in the embryo and its subsequent abortion.
Daniely
M
M
The Institute of Human Genetics, and Dept. of Obstetrics and Gynecology, Sheba Medical Center, Tel-Hashomer, Israel.
Aviram
A
A
Carp
H J
HJ
Shaki
R
R
Barkai
G
G
D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D000782:Aneuploidy; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D046508:Culture Techniques; D005260:Female; D005820:Genetic Testing; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D010290:Parents; D010920:Placenta / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D011247:Pregnancy