The measurement of parasite antigen-specific IgE levels using anti-IgE monoclonal antibodies and biotinylated antigens.
We studied and evaluated an ELISA system, using a sandwich method with a monoclonal antibody against the Fc domain of IgE molecules and biotinylated antigens, to detect parasite antigen-specific IgE quantitatively. The specific IgE ELISA titre increases linearly in a dose-dependent manner when the concentration of total IgE in samples is less than 2,000 ng/ml. Sera from IgE-deficient SJA/9 mice infected with Trichinella spiralis failed to give any measurable IgE, suggesting that other classes of immunoglobulins have no effect on this assay. The titre showed a good correlation with PCA titre. A high concentration of the serum from Toxocara canis-infected mice reduced the T. spiralis-specific IgE ELISA titre, suggesting that the ELISA system is influenced by a huge amount of IgE against epitopes different from those of target antigens. This ELISA system can also be applied for detecting other classes or subclasses of antigen-specific immunoglobulins.
Takamoto
M
M
Department of Immunology and Infectious Disease, Shinshu University Graduate School of Medicine, Matsumoto, Japan. masayat@sch.md.shinshu-u.ac.jp
Wang
Z X
ZX
Watanabe
N
N
Sugane
K
K
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000276:immunology*; D019921:Biotinylation / Q000379:methods; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007073:Immunoglobulin E / Q000032:analysis* / Q000172:deficiency / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D051381:Rats; D017166:Toxocara canis / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007073:Immunoglobulin E