Delta opioid receptors mediate glucose uptake in skeletal muscles of lean and obese-diabetic (ob/ob) mice.
Specific binding sites for [125I]beta-endorphin and the delta1-opioid [3H][D-pen(2), D-pen(5)]enkephalin (DPDPE) were quantified using autoradiography in soleus and extensor digitorum longus (EDL) muscles of lean and obese-diabetic (ob/ob) mice. The density of binding was significantly higher in obese-diabetic than lean mice. The uptake of 2-deoxy-D-[1-3H]deoxyglucose, a nonmetabolized glucose analogue, into isolated soleus and EDL muscles was stimulated by beta-endorphin, beta-endorphin 1-27, and DPDPE, but not by the delta2-opioid deltorphin II. Both beta-endorphin and DPDPE stimulated deoxyglucose uptake in obese-diabetic mice. Thus, glucose transport in skeletal muscle may be partly mediated via delta1-opioid receptors. The increased receptor density in obese-diabetic mice may be an adaptive response.
Evans
A A
AA
Department of Physiology, Medical School, University of Birmingham, Birmingham, UK.
Tunnicliffe
G
G
Knights
P
P
Bailey
C J
CJ
Smith
M E
ME
D000818:Animals; D001345:Autoradiography; D001786:Blood Glucose / Q000032:analysis; D003847:Deoxyglucose / Q000378:metabolism; D003920:Diabetes Mellitus / Q000378:metabolism*; D020881:Enkephalin, D-Penicillamine (2,5)- / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000378:metabolism*; D009765:Obesity; D017465:Receptors, Opioid, delta / Q000502:physiology*; D014316:Tritium; D001615:beta-Endorphin / Q000378:metabolism
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D007457:Iodine Radioisotopes; D017465:Receptors, Opioid, delta; D014316:Tritium; D001615:beta-Endorphin; D020881:Enkephalin, D-Penicillamine (2,5)-; D003847:Deoxyglucose; D005947:Glucose