Measurement of Ca(2+) currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique.
The recording of currents passing through calcium channels in intact skeletal muscle fibers presents several difficulties. However, use of the three-microelectrode voltage-clamp technique at the end of the fiber provided us with a good approximation of current values in such fibers. Using this technique, we were able to measure the calcium-channel current in slow skeletal muscle fibers of the frog (Rana pipiens) and to quantify the effects of denervation on this current.
Vásquez
C
C
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Apartado Postal No. 11, 28000-Colima, Colima, Mexico.
Huerta
M
M
Trujillo
X
X
Luis Marín
J
J
Andrade
F
F
Trujillo-Hernández
B
B
D000818:Animals; D001464:Barium; D015220:Calcium Channels / Q000378:metabolism*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008839:Microelectrodes; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism* / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D018408:Patch-Clamp Techniques; D011894:Rana pipiens; D009435:Synaptic Transmission / Q000502:physiology
D015220:Calcium Channels; D001464:Barium