In vitro cytotoxicity of six dentin bonding agents.
The cytotoxicity of six dentin bonding agents (Syntac, Solobond, Bond 1, Scotchbond 1, Heliobond and F-2000) was tested against an established cell line, L929. Under aseptic conditions 3, 5 and 10 microL dentin bonding agents were placed in the centre of Petri dishes. Each dish was covered with a 5-mL suspension of fibroblasts at a concentration of 40 000 cells mL(-1). The cultures were incubated at 37 degrees C and cytotoxicity was assessed by a quantitative technique at 24 and 72 h. All the dentin bonding agents were found to be cytotoxic. Scotchbond 1 and F-2000 showed the highest cytotoxicity followed by Solobond and Bond 1. Heliobond and Syntac were the least toxic materials.
Koliniotou-Koubia
E
E
Dental School, Department of Operative Dentistry and Theagenion Cancer Institute, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece.
Dionysopoulos
P
P
Koulaouzidou
E A
EA
Kortsaris
A H
AH
Papadogiannis
Y
Y
D000179:Acrylates / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D020770:Compomers / Q000633:toxicity; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity; D007739:L Cells; D008689:Methacrylates / Q000633:toxicity; D051379:Mice; D019279:Resin Cements / Q000633:toxicity
D000179:Acrylates; C115611:BOND-1 adhesive; D020770:Compomers; D017220:Dentin-Bonding Agents; C118368:F2000 cement; D005899:Glass Ionomer Cements; D008689:Methacrylates; D019279:Resin Cements; C425825:Solobond M; C078484:Syntac dentine adhesive; C040415:heliobond; C041330:Scotchbond