title: Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds.
abstract: Immunolocalization of alkaloids in lupin seeds (Lupinus spp.) has been performed by cryofixation and conventional methods. Alkaloids were localized in the protein bodies of the cotyledon cells. Some immunogold particles in the walls of these cells were also observed. There were no differences in the sites of localization between the two mentioned methods. X-ray microanalysis of elements showed the presence of P, Mg, S, and K in the protein bodies of cotyledon cells in lupin seeds. The role of K+ in alkaloids transport is discussed.
authors.affiliation: Departamento de Fisiología y Bioquímica Vegetal, Centro de Ciencias Medioambientales, CSIC, Serrano 115 Dpdo, 28006 Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Pozuelo
authors.forename: M M
authors.initials: MM
authors.lastname: Lucas
authors.forename: C
authors.initials: C
authors.lastname: de Lorenzo
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Pascual
authors.forename: S
authors.initials: S
authors.lastname: Maldonado
authors.forename: M R
authors.initials: MR
authors.lastname: de Felipe
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D018548:Cotyledon / Q000737:chemistry / Q000648:ultrastructure; D004577:Electron Probe Microanalysis; D007887:Fabaceae / Q000737:chemistry* / Q000648:ultrastructure; D007150:Immunohistochemistry; D010940:Plant Proteins / Q000032:analysis; D012639:Seeds / Q000737:chemistry* / Q000648:ultrastructure; D013034:Sparteine / Q000031:analogs & derivatives
chemical_list: D000470:Alkaloids; D010940:Plant Proteins; C008298:lupanine; D013034:Sparteine