CD30L up-regulates CD30 and IL-4 expression by T cells.
CD30L is frequently expressed on acute myeloid leukemia (AML) blasts. Its presence is associated with the co-expression of interleukin-4 (IL-4) receptor and with the expansion of specific T-helper 2 (Th2) cell subsets producing IL-4 and expressing CD30. Recombinant CD30L-bearing cells up-regulated the expression of surface CD30 and increased the production of IL-4 and soluble (s) CD30 by co-cultured T cells. These findings were confirmed with AML blasts expressing surface CD30L, where blocking anti-CD30 antibodies completely abolished the release of sCD30 and reduced the production of IL-4. Our data indicates a direct role of CD30L(+) neoplastic cells in driving the immune response toward a Th2-polarized non-protective state.
Rossi
F M
FM
Clinical and Experimental Hematology Research Unit, Centro di Riferimento Oncologico, I.R.C.C.S., Via Pedemontana Occ.le 12, 33081 (PN), Aviano, Italy.
Degan
M
M
Mazzocut-Zecchin
L
L
Di Francia
R
R
Aldinucci
D
D
Pinto
A
A
Gattei
V
V
D000208:Acute Disease; D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D053285:CD30 Ligand; D018920:Coculture Techniques; D006801:Humans; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics*; D017730:Ki-1 Antigen / Q000096:biosynthesis / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000276:immunology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000502:physiology*; D011784:Quail; D011994:Recombinant Proteins / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D017252:CD3 Complex; D053285:CD30 Ligand; D017730:Ki-1 Antigen; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C505774:TNFSF8 protein, human; D015847:Interleukin-4