title: Enhancement of inward Ca(2+) currents in bovine chromaffin cells by green tea polyphenol extracts.
abstract: Green tea contains four major polyphenol compounds: they are (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (ECG), and (-)-epicatechin (EC). Although all four polyphenol compounds are known to affect tumor suppression, little is known about whether they alter membrane properties. In this study, we examined the effects of ECG and EGCG on ionic currents and secretion. Membrane capacitance changes were used to monitor secretion in bovine chromaffin cells. ECG had the ability to reversibly enhance the inward Ca(2+) current by 21%, and inhibited the peak sodium current by 34%. EGCG had no effect on Ca(2+) current even though it differs from ECG by just a hydroxyl group. The EC(50) of ECG in enhancing Ca(2+) current was 7.6 microM. The maximum enhancement of Ca(2+) current was observed at 0 mV and the maximum current was shifted approximately 10 mV in the hyperpolarizing direction. When cells were stimulated by trains of depolarizations, the exocytosis elicited was enhanced by ECG treatment and the largest enhancement of secretion was observed in later stimulations. EGCG, although it had no significant effect on Ca(2+) current, enhanced exocytosis and slowed endocytosis. These results suggest that green tea polyphenol compounds modulate stimulus-secretion coupling in bovine chromaffin cells.
authors.affiliation: Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, 947 East 58th Street, Chicago, IL 60637, USA. cypan@drugs.bsd.uchicago.edu
authors.forename: Chien Yuan
authors.initials: CY
authors.lastname: Pan
authors.forename: Yung Hsi
authors.initials: YH
authors.lastname: Kao
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Fox
mesh_terms: D000313:Adrenal Medulla / Q000502:physiology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002417:Cattle; D019439:Chromaffin Cells / Q000187:drug effects / Q000502:physiology*; D005419:Flavonoids; D066298:In Vitro Techniques; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D011108:Polymers; D002392:Catechin; C062669:epicatechin gallate