The assignment by linkage mapping of four genes from human chromosome 22 to bovine chromosome 5 and 17.
Polymerase chain reaction oligonucleotides were designed to amplify bovine specific sequences for four genes that are located on human chromosome 22 (HSA22): crystallin beta A4 (CRY B A4), parvalbumin (PVALB), tissue inhibitor of metalloproteinase 3 (TIMP3) and matrix metalloproteinase 11 (MMP11). Single strand conformation analysis of these bovine gene fragments defined polymorphisms within a population of three large half-sib families of three F1 Charolais x Brahman sires and a composite herd comprising an equal proportion of Africander, Brahman, Hereford and Shorthorn breeds (CSIRO pedigree). The DNA marker genotypes were used to define linkage associations to other DNA markers already placed on the CSIRO linkage map. The genes TIMP3 and PVALB were assigned to BTA5 and CRYbetaA4 and MMP11 to BTA17.
Ariza
F
F
Molecular Animal Genetics Centre, CSIRO, University of Queensland, Brisbane, Australia. marizabo@visto.com
Harrison
B
B
Drinkwater
R D
RD
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary; D002892:Chromosomes, Human, Pair 22; D003459:Crystallins / Q000235:genetics; D006801:Humans; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases / Q000235:genetics; D010320:Parvalbumins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D026801:Synteny; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics; D038205:beta-Crystallin A Chain
C103907:CRYBA4 protein, human; D003459:Crystallins; D010320:Parvalbumins; D019717:Tissue Inhibitor of Metalloproteinase-3; D038205:beta-Crystallin A Chain; D053507:Matrix Metalloproteinase 11; D008666:Metalloendopeptidases