title: Computational characterization of substrate binding and catalysis in S-adenosylhomocysteine hydrolase.
abstract: S-Adenosylhomocysteine (AdoHcy) hydrolase catalyzes the reversible hydrolysis of AdoHcy to adenosine (Ado) and homocysteine (Hcy), playing an essential role in modulating the cellular Hcy levels and regulating activities of a host of methyltransferases in eukaryotic cells. This enzyme exists in an open conformation (active site unoccupied) and a closed conformation (active site occupied with substrate or inhibitor) [Turner, M. A., Yang, X., Yin, D., Kuczera, K., Borchardt, R. T., and Howell, P. L. (2000) Cell Biochem. Biophys. 33, 101-125]. To investigate the binding of natural substrates during catalysis, the computational docking program AutoDock (with confirming calculations using CHARMM) was used to predict the binding modes of various substrates or inhibitors with the closed and open forms of AdoHcy hydrolase. The results have revealed that the interaction between a substrate and the open form of the enzyme is nonspecific, whereas the binding of the substrate in the closed form is highly specific with the adenine moiety of a substrate as the main recognition factor. Residues Thr57, Glu59, Glu156, Gln181, Lys186, Asp190, Met351, and His35 are involved in substrate binding, which is consistent with the crystal structure. His55 in the docked model appears to participate in the elimination of water from Ado through the interaction with the 5'-OH group of Ado. In the same reaction, Asp131 removes a proton from the 4' position of the substrate after the oxidation-reduction reaction in the enzyme. To identify the residues that bind the Hcy moiety, AdoHcy was docked to the closed form of AdoHcy hydrolase. The Hcy tail is predicted to interact with His55, Cys79, Asn80, Asp131, Asp134, and Leu344 in a strained conformation, which may lower the reaction barrier and enhance the catalysis rate.
authors.affiliation: Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence, Kansas 66045-2106, USA. ybhu@ku.edu
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: D H
authors.initials: DH
authors.lastname: Yin
authors.forename: J
authors.initials: J
authors.lastname: Mahadevan
authors.forename: K
authors.initials: K
authors.lastname: Kuczera
authors.forename: R L
authors.initials: RL
authors.lastname: Schowen
authors.forename: R T
authors.initials: RT
authors.lastname: Borchardt
mesh_terms: D000241:Adenosine / Q000378:metabolism; D043383:Adenosylhomocysteinase; D000818:Animals; D002384:Catalysis; D020134:Catalytic Domain; D003198:Computer Simulation; D006801:Humans; D006867:Hydrolases / Q000737:chemistry* / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008958:Models, Molecular; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism; D012984:Software; D013379:Substrate Specificity; D013816:Thermodynamics
chemical_list: D012435:S-Adenosylhomocysteine; D006867:Hydrolases; D043383:Adenosylhomocysteinase; D000241:Adenosine