Effects and mechanisms of triacetylshikimic acid on platelet adhesion to neutrophils induced by thrombin and reperfusion after focal cerebral ischemia in rats.
OBJECTIVE
To investigate the effect of triacetylshikimic acid (TSA) on the platelet adhesion to neutrophils and P-selectin expression on activated platelet membrane induced by thrombin and reperfusion after focal cerebral ischemia.


METHODS
The platelet adhesion to neutrophils was evaluated by rosette assay, and P-selectin expression on platelet membrane was determined by flow cytometry.


RESULTS
TSA 10 - 1000 micromol/L markedly inhibited thrombin(0.4 kU/L)-induced platelet adhesion to neutrophils. The platelet adhesion to neutrophils induced by a 21-h reperfusion after middle cerebral artery occlusion for 3 h was also inhibited in a dose-dependent manner by TSA 50 - 200 mg/kg given by ig immediately and at 60 min again after the onset of cerebral ischemia. TSA was also shown to decrease the P-selectin expression on platelet surface induced by thrombin in washed platelet and by adenosine diphosphate (ADP) 5 micromol/L in whole blood.


CONCLUSIONS
Reperfusion after cerebral ischemia and thrombin induced platelet adhesion to neutrophils, which could be reduced by TSA probably due to its inhibition of P-selectin expression on activated platelets.
Chong
Z Z
ZZ
Department of Pharmacology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Xu
Q P
QP
Sun
J N
JN
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D020244:Infarction, Middle Cerebral Artery / Q000097:blood*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology*; D019007:P-Selectin / Q000096:biosynthesis; D010973:Platelet Adhesiveness / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*
D018696:Neuroprotective Agents; D019007:P-Selectin; C452560:triacetylshikimic acid; D012765:Shikimic Acid; D013917:Thrombin