title: Age-dependent changes in 24-hour rhythms of catecholamine content and turnover in hypothalamus, corpus striatum and pituitary gland of rats injected with Freund's adjuvant.
abstract: BACKGROUND
  Little information is available on the circadian sequela of an immune challenge in the brain of aged rats. To assess them, we studied 24-hour rhythms in hypothalamic and striatal norepinephrine (NE) content, hypothalamic and striatal dopamine (DA) turnover and hypophysial NE and DA content, in young (2 months) and aged (18-20 months) rats killed at 6 different time intervals, on day 18th after Freund's adjuvant or adjuvant's vehicle administration.
  
  
  RESULTS
  Aging decreased anterior and medial hypothalamic NE content, medial and posterior hypothalamic DA turnover, and striatal NE concentration and DA turnover. Aging also decreased NE and DA content in pituitary neurointermediate lobe and augmented DA content in the anterior pituitary lobe. Immunization by Freund's adjuvant injection caused: (i) reduction of DA turnover in anterior hypothalamus and corpus striatum; (ii) acrophase delay of medial hypothalamic DA turnover in old rats, and of striatal NE content in young rats; (iii) abolition of 24-h rhythm in NE and DA content of neurointermediate pituitary lobe, and in DA content of anterior lobe, of old rats.
  
  
  CONCLUSIONS
  The decline in catecholamine neurotransmission with aging could contribute to the decrease of gonadotropin and increase of prolactin release reported in similar groups of rats. Some circadian responses to immunization, e.g. suppression of 24-h rhythms of neurointermediate lobe NE and DA and of anterior lobe DA were seen only in aged rats.
authors.affiliation: Departamento de Bioquímica y Biología Molecular III, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Cano
authors.forename: D P
authors.initials: DP
authors.lastname: Cardinali
authors.forename: F
authors.initials: F
authors.lastname: Chacon
authors.forename: P O
authors.initials: PO
authors.lastname: Castrillón
authors.forename: C A
authors.initials: CA
authors.lastname: Reyes Toso
authors.forename: A I
authors.initials: AI
authors.lastname: Esquifino
mesh_terms: D000375:Aging; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002940:Circadian Rhythm; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007267:Injections; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002395:Catecholamines; D005620:Freund's Adjuvant; D004298:Dopamine; D009638:Norepinephrine