title: Detection of Mycoplasma fermentans in broncho-alveolar lavage fluid specimens from AIDS patients with lower respiratory tract infection.
abstract: OBJECTIVE
  To investigate the role of Mycoplasma fermentans in lower respiratory tract disease of HIV-positive patients.
  
  
  METHODS
  A polymerase chain reaction (PCR) assay was used to detect M. fermentans in broncho-alveolar lavage (BAL) fluid and peripheral blood mononuclear cells (PBMCs) collected from 78 hospitalized HIV-positive patients with lower respiratory tract disease (the index group). BAL specimens were also assessed from two control groups; 25 HIV-positive patients without respiratory symptoms and 46 patients with a respiratory illness who did not have risk factors for HIV.
  
  
  RESULTS
  Seventy-one (91%) of the 78 patients who comprised the index group had AIDS. Sixty-one patients provided BAL and PBMC specimens; 16 provided a BAL specimen and one only a PBMC specimen; 15 (25%) of the 61 patients had M. fermentans positive BAL fluid, of whom 10 (67%) also had a positive PBMC specimen. In contrast, of the 46 patients who had a negative BAL fluid, only three (6.4%) had a positive PBMC sample (P < 0.00001 Fisher's exact test). Overall, 21 (27%) of 77 patients had M. fermentans positive BAL fluid. Of these positive patients, 16 also had another microorganism in the BAL fluid and for five patients M. fermentans was detected alone. Compared with patients in the index group, none of the 46 BAL fluid specimens from patients at low risk of HIV infection was M. fermentans positive (P < 0.001, chi2 15.13). Two (8%) of the 25 HIV-positive patients without respiratory symptoms had a M. fermentans positive BAL fluid, positivity that was also statistically different from the index group (P = 0.045, chi2 4.01).
  
  
  CONCLUSIONS
  M. fermentans frequently invades the lower respiratory tract of AIDS patients as an opportunist and may cause disease in some.
authors.affiliation: Sections of Genitourinary Medicine and Respiratory Medicine, Division of Medicine, Imperial College School of Medicine, St Mary's Hospital, London, UK.
authors.forename: J G
authors.initials: JG
authors.lastname: Ainsworth
authors.forename: J
authors.initials: J
authors.lastname: Clarke
authors.forename: M
authors.initials: M
authors.lastname: Lipman
authors.forename: D
authors.initials: D
authors.lastname: Mitchell
authors.forename: D
authors.initials: D
authors.lastname: Taylor-Robinson
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology*; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000382:microbiology*; D016858:Mycoplasma fermentans / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000382:microbiology*; D012189:Retrospective Studies