Chaperone-like properties of lysophospholipids.
Lysophospholipids are metabolic intermediates in phospholipid turnover, detergent molecules with membrane-modulating effects, and multifunctional cellular growth factors in eukaryotic cells. In bacterial cells, lysophospholipids are mostly found in the form of lysophosphatidylethanolamine. We show that a heat shock from 30 to 42 degrees C increases four-fold the Escherichia coli pool of lysophosphoethanolamine and that lysophospholipids display chaperone-like properties. Lysophosphatidylethanolamine, like molecular chaperones such as DnaK, promotes the functional folding of citrate synthase and alpha-glucosidase after urea denaturation. Like chaperones, lysophophatidylethanolamine, lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidic acid prevent the aggregation of citrate synthase at 42 degrees C. The renaturation and solubilisation of proteins by lysophospholipids occur at micromolar concentrations of these compounds, close to their critical micellar concentration. Furthermore, lysophosphatidylethanolamine is much more efficient than other detergents tested for the renaturation and solubilisation of citrate synthase. In contrast with lysophospholipids, phosphatidylethanolamine and phosphatidylcholine are not able to promote citrate synthase folding nor to prevent its aggregation at 42 degrees C. The chaperone-like properties of lysophospholipids suggest that, in addition to their known functions, they might affect the structure and function of hydrophilic proteins.
Kern
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R
Stress Molecules, Institut Jacques Monod, Université Paris 7, Paris, 2 place Jussieu, 75005, France.
Joseleau-Petit
D
D
Chattopadhyay
M K
MK
Richarme
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G
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D006358:Hot Temperature; D008246:Lysophospholipids / Q000378:metabolism* / Q000494:pharmacology; D018832:Molecular Chaperones / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology; D017510:Protein Folding; D020673:Protein Renaturation / Q000187:drug effects; D012995:Solubility; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
D001426:Bacterial Proteins; D003902:Detergents; D008246:Lysophospholipids; D018832:Molecular Chaperones; D010743:Phospholipids; C008301:lysophosphatidylethanolamine; D002950:Citrate (si)-Synthase; D000520:alpha-Glucosidases