Effect of phenolic alkaloids from Menispermum dauricum on myocardial-cerebral ischemia-reperfusion injury in rabbits.
OBJECTIVE
To explore the mechanism underlying the effect of phenolic alkaloids from Menispermum dauricum (PAMd) on simultaneous myocardial-cerebral ischemia-reperfusion injury in rabbits.


METHODS
Both left anterior descending coronary artery and bilateral carotid arteries were occluded to induce myocardial-cerebral ischemia-reperfusion injury in rabbits. At 30 min after ischemia, the occlusion was removed and shed blood was rapidly reinfused. Two mL of blood was taken fr om femoral artery at 10 min before ischemia, 1, 10, and 30 min after ischemia, and 1, 10, 30, 60, 120, 180, and 240 min after reperfusion. Each rabbit was sacrificed at the end of reperfusion, and left ventricle, hippocampus, cortex, and cerebellum were taken out. Malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined.


RESULTS
At 10 min after reperfusion, MDA content in serum was significantly higher and SOD activity was lower in ischemia-reperfusion (I-R) group than those of control group (P < 0.05). After administration of PAMd, MDA content was lower and SOD activity was higher in serum than those of I-R group (P < 0.05). Both MDA content and SOD activity in tissues had the similar results with those in serum.


CONCLUSIONS
PAMd could attenuate the injury induced by lipid peroxidation and enhance the activity of SOD, thus PAMd might play a protective role in simultaneous myocardial-cerebral ischemia-reperfusion injury.
Wang
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Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. wangfang0322@yahoo.com.cn
Qu
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Lv
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Guo
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LJ
D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D002545:Brain Ischemia / Q000097:blood / Q000517:prevention & control*; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D031603:Menispermum / Q000737:chemistry*; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D018517:Plant Roots / Q000737:chemistry; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000097:blood / Q000517:prevention & control; D013482:Superoxide Dismutase / Q000097:blood
D000470:Alkaloids; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C017616:phenolic acid