title: Role of STAT6 in IgE receptor/FcepsilonRI-mediated late phase allergic responses of mast cells.
abstract: In this study we show that IgE receptor engagement triggers activation of STAT6 in mast cells. We sought to determine the role of STAT6 activation in IgE receptor-mediated mast cell responses using STAT6 knockout mice. After IgE receptor engagement, bone marrow mast cells from STAT6(-/-) mice exhibited normal histamine and leukotriene C(4) release, but their cytokine release was markedly reduced. In accordance with these in vitro data, IgE/Ag-challenged STAT6(-/-) mice showed normal early phase, but severely impaired late phase, allergic reactions. These findings provide unprecedented evidence that STAT6 plays a pivotal role in mast cell responses to IgE/Ag stimulation.
authors.affiliation: Department of Allergy, Parker Hughes Institute, 2699 Patton Road, St. Paul, MN 55113, USA.
authors.forename: Ravi
authors.initials: R
authors.lastname: Malaviya
authors.forename: Fatih M
authors.initials: FM
authors.lastname: Uckun
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006636:Histamine Release; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000473:pathology; D007073:Immunoglobulin E / Q000276:immunology; D007700:Kinetics; D017997:Leukotriene C4 / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology; D017455:Receptors, IgE / Q000276:immunology*; D050800:STAT6 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*
chemical_list: D016207:Cytokines; D017455:Receptors, IgE; D050800:STAT6 Transcription Factor; C494100:Stat6 protein, mouse; D015534:Trans-Activators; D017997:Leukotriene C4; D007073:Immunoglobulin E