title: Action of brassinosteroids in the cotton leafworm Spodoptera littoralis.
abstract: The two native plant hormones 24-epibrassinolide and 24-epicastasterone showed 50% competition for binding at IC(50) of 1-3.6 microM with [(3)H]ponasterone A using cultured imaginal wing discs from last-instar larvae of the cotton leafworm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae). However, culture of imaginal wing discs in different concentrations of brassinosteroids, even up to 100 microM, demonstrated no induction of evagination. In contrast, 20E and the non-steroidal agonist RH-5992 competed respectively about 23- and 42-fold more effectively with labeled ponasterone A, and their ability (EC(50)) to induce disc evagination in vitro was 158 and 87 nM, respectively. Injection of 10 microg of brassinosteroids in newly-moulted last-instar larvae did not cause mortality above controls; higher mortalities were scored when brassinosteroids were injected late in the last instar.
authors.affiliation: Laboratory of Agrozoology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. guy.smagghe@rug.ac.be
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
authors.forename: L
authors.initials: L
authors.lastname: Decombel
authors.forename: B
authors.initials: B
authors.lastname: Carton
authors.forename: B
authors.initials: B
authors.lastname: Voigt
authors.forename: G
authors.initials: G
authors.lastname: Adam
authors.forename: L
authors.initials: L
authors.lastname: Tirry
mesh_terms: D000818:Animals; D060406:Brassinosteroids; D002777:Cholestanols / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D004441:Ecdysterone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D005247:Feeding Behavior; D006834:Hydrazines / Q000378:metabolism; D007267:Injections; D007814:Larva; D015394:Molecular Structure; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D018411:Spodoptera / Q000187:drug effects; D013260:Steroids, Heterocyclic / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D060406:Brassinosteroids; D002777:Cholestanols; D006834:Hydrazines; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; D004441:Ecdysterone; C083545:castasterone; C008999:ponasterone A; C082026:tebufenozide; C023623:brassinolide