Lack of binding to isolated estrogen or androgen receptors, and inactivity in the immature rat uterotrophic assay, of the ultraviolet sunscreen filters Tinosorb M-active and Tinosorb S.
The presence of structurally diverse chemicals as contaminants in the environment has led to concerns regarding their possible endocrine disturbing effects. Recently, some ultraviolet absorbing components of sunscreen preparations have given positive responses in assays monitoring estrogen-like activity both in vitro and in vivo. Consequently, two recently developed sunscreen components, Tinosorb M-active and Tinosorb S, were evaluated using the in vitro estrogen and androgen receptor competitive binding assays. Neither compound gave a positive response in either of the assays, consistent with the large molecular dimensions of each chemical disfavoring binding to the hormone receptors. Both of the chemicals were inactive in immature rat uterotrophic assays conducted using the subcutaneous route of administration. It is concluded that neither of these agents possess intrinsic estrogenic/antiestrogenic or androgenic/antiandrogenic activity. The several positive control chemicals evaluated gave the expected positive responses in the assays used.
Ashby
J
J
Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, United Kingdom. John.Ashby@Syngenta.com
Tinwell
H
H
Plautz
J
J
Twomey
K
K
Lefevre
P A
PA
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D004702:Endocrine Glands / Q000187:drug effects; D004781:Environmental Exposure; D004958:Estradiol / Q000378:metabolism; D005260:Female; D007279:Injections, Subcutaneous; D008297:Male; D010636:Phenols / Q000737:chemistry / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000187:drug effects* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000378:metabolism; D013473:Sunscreening Agents / Q000737:chemistry / Q000633:toxicity*; D013739:Testosterone / Q000378:metabolism; D014227:Triazines / Q000737:chemistry / Q000633:toxicity*; D014466:Ultraviolet Rays; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D010636:Phenols; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D013473:Sunscreening Agents; D014227:Triazines; D013739:Testosterone; D004958:Estradiol; C439083:bis-ethylhexyloxyphenol methoxyphenyl triazine