Diagnosis of Oropouche virus infection by RT-nested-PCR.
Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.
Moreli
Marcos Lázaro
ML
Virus Research Unit, School of Medicine of Ribeirão Preto, University of São Paulo, Campus da USP, Ribeirão Preto, SP, Brazil.
Aquino
Victor Hugo
VH
Cruz
Ana Cecília R
AC
Figueiredo
Luiz Tadeu M
LT
D001483:Base Sequence; D002044:Bunyaviridae Infections / Q000175:diagnosis*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012840:Simbu virus / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral