title: Crystallization and preliminary X-ray diffraction analysis of monkey dimeric dihydrodiol dehydrogenase.
abstract: Dihydrodiol dehydrogenase catalyzes the NADP(+)-linked oxidation of trans-dihydrodiols of aromatic hydrocarbons to corresponding catechols and exists in multiple forms in mammalian tissues. The dimeric form of mammalian dihydrodiol dehydrogenase has a primary structure distinct from the previously known mammalian enzymes and may constitute a novel protein family with the prokaryotic proteins. Monkey kidney dimeric dihydrodiol dehydrogenase was crystallized from buffered ammonium phosphate solution using the hanging-drop vapour-diffusion method. The crystals diffract to 2.65 A resolution in the laboratory and belong to the hexagonal P6(1)22 or P6(5)22 space group, with unit-cell parameters a = b = 122.8, c = 121.3 A, alpha = beta = 90, gamma = 120 degrees.
authors.affiliation: Department of Medicinal Chemistry, Victorian College of Pharmacy, Monash University (Parkville Campus), Parkville, Victoria 3052, Australia. ossama.el-kabbani@vcp.monash.edu.au
authors.forename: Ossama
authors.initials: O
authors.lastname: El-Kabbani
authors.forename: Roland P T
authors.initials: RP
authors.lastname: Chung
authors.forename: Paul A
authors.initials: PA
authors.lastname: Ramsland
authors.forename: Yukuhiko
authors.initials: Y
authors.lastname: Asada
authors.forename: Syuhei
authors.initials: S
authors.lastname: Ishikura
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Usami
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D008251:Macaca; D010088:Oxidoreductases / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D011994:Recombinant Proteins; D010088:Oxidoreductases; C048338:trans-1,2-dihydrobenzene-1,2-diol dehydrogenase