Structure of cell clusters formed in cultures of dissociated human embryonic brain.
Cell clusters in a culture of dissociated brain from human fetuses at 8-12 weeks gestation in a serum-free growth medium were studied by immunohistochemical methods and electron microscopy. Heterogeneity of cell population in culture was demonstrated. Despite the influence of proliferation-stimulating factors, cell clusters contained not only nestin-immunopositive stem cells, but also beta-tubulin-, vimentin-, and GFAP-positive cells differentiating by the neural pathway. Stem cells were localized on the surface of clusters. The percentage of stem cells in large clusters was lower than in small clusters.
Revishchin
A V
AV
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Poltavtseva
R A
RA
Marei
M V
MV
Aleksandrova
M A
MA
Viktorov
I V
IV
Korochkin
L I
LI
Sukhikh
G T
GT
D001921:Brain / Q000166:cytology* / Q000196:embryology* / Q000648:ultrastructure; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D008854:Microscopy, Electron; D009419:Nerve Tissue Proteins; D064231:Nestin; D009474:Neurons / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D013997:Time Factors; D014404:Tubulin / Q000096:biosynthesis; D014746:Vimentin / Q000096:biosynthesis
D016895:Culture Media, Serum-Free; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014404:Tubulin; D014746:Vimentin