Direct assay of Aplysia tissues and cells with laser desorption/ionization mass spectrometry on porous silicon.
Desorption/ionization on porous silicon (DIOS) is a form of laser desorption mass spectrometry that allows for the direct mass analysis of a variety of analytes without the addition of organic matrix. Protocols are described for the direct analysis of exocrine tissue and single neurons using DIOS-MS. The atrial gland of Aplysia californica was blotted on to porous silicon and analyzed with DIOS-MS in the range m/z 1000-4000. The ability to culture invertebrate neurons directly on porous silicon is also presented. Isolated bag cells regenerated neuronal processes in culture on porous silicon. DIOS-MS allowed the direct detection of the peptides contained in individual cultured neurons indicating that with appropriate protocols, DIOS can be used with biological samples with considerable thickness.
Kruse
R A
RA
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Rubakhin
S S
SS
Romanova
E V
EV
Bohn
P W
PW
Sweedler
J V
JV
D000818:Animals; D001048:Aplysia / Q000737:chemistry*; D002478:Cells, Cultured; D005088:Exocrine Glands / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000737:chemistry; D009479:Neuropeptides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012825:Silicon; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D009479:Neuropeptides; D010455:Peptides; D012825:Silicon