Keratan sulphate in cerebrum, cerebellum and brainstem of sheep brain.
Keratan sulphate was identified in sheep brain. We describe here the isolation and partial characterization of keratan sulphate from cerebrum, cerebellum and brainstem of young sheep brains. The galactosaminoglycan was isolated by using ion-exchange chromatography and gel filtration after exhaustive digestion with papain of the delipidated tissues, followed by alkaline borohydride degradation and chondroitinase ABC and heparinases I, II and III treatment. The material isolated by ion-exchange chromatography from each tissue was eluted as single but polydispersed peak from Sephadex G-75, with average molecular masses 8.4, 7.9 and 8.8 kDa for cerebrum, cerebellum and brainstem, respectively. Keratanase I and II totally degraded keratan sulphate from cerebrum and brainstem, but only partially that from cerebellum. The content of keratan sulphate was found to be about 215, 173 and 144 microg/g dry delipidated tissue for cerebrum, brainstem and cerebellum, respectively.
Papageorgakopoulou
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Department of Chemistry, Organic Chemistry, Biochemistry and Natural Products Division, University of Patras, Patras 26500, Greece. papageorg.letta@chemistry.upatras.gr
Theocharis
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Skandalis
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Vynios
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Theocharis
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Tsiganos
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D000818:Animals; D001933:Brain Stem / Q000737:chemistry*; D002531:Cerebellum / Q000737:chemistry*; D006026:Glycoside Hydrolases; D007632:Keratan Sulfate / Q000032:analysis / Q000302:isolation & purification*; D011509:Proteoglycans / Q000737:chemistry; D012756:Sheep; D013687:Telencephalon / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism
D011509:Proteoglycans; D007632:Keratan Sulfate; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase