title: Lecithin: cholesterol acyltransferase is insufficient to prevent oxidative modification of low-density lipoprotein.
abstract: We tried to confirm the antioxidative capability of lecithin:cholesterol acyltransferase (LCAT) reported by Vohl et al. [Biochemistry (1999) 38, 5976-5981]. The enzyme solution protected LDL against oxidation. However, this protection was not due to LCAT enzyme, but to some unknown low-molecular-weight substance(s) in the solution; LCAT itself exerted little protective effect against LDL oxidation.
authors.affiliation: Laboratory of Nutrition, Graduate School of Agricultural Science, and Gene Research Center, Tohoku University, Tsutsumidori, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Murakami
authors.forename: Shin
authors.initials: S
authors.lastname: Kamiyama
authors.forename: Zakir H
authors.initials: ZH
authors.lastname: Howlader
authors.forename: Tokuhisa
authors.initials: T
authors.lastname: Yamato
authors.forename: Michio
authors.initials: M
authors.lastname: Komai
authors.forename: Yuji
authors.initials: Y
authors.lastname: Furukawa
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007531:Isoflurophate / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011489:Protein Denaturation
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D008077:Lipoproteins, LDL; D007531:Isoflurophate; D004228:Dithionitrobenzoic Acid; D007862:Phosphatidylcholine-Sterol O-Acyltransferase