Biochemical engineering of the production of plant-specific secondary metabolites by cell suspension cultures.
Plant cell culture has recently received much attention as a useful technology for the production of valuable plant-derived secondary metabolites such as paclitaxel and ginseng saponin. The numerous problems that yet bewilder the optimization and scale-up of this process have not been over emphasized. In spite of the great progress recorded in recent years towards the selection, design and optimization of bioreactor hardware, manipulation of environmental factors such as medium components, light irradiation, shear stress and O2 supply needs detailed investigations for each case. Recent advances in plant cell processes, including high-density suspension cultivation, continuous culture, process monitoring, modeling and control and scale-up, are also reviewed in this chapter. Further developments in bioreactor cultivation processes and in metabolic engineering of plant cells for metabolite production are expected in the near future.
Zhong
J J
JJ
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. jjzhong@ecust.edu.cn
D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D008027:Light; D059828:Plant Cells; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D013314:Stress, Mechanical
D010936:Plant Extracts