title: Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts.
abstract: The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcgamma-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcgamma-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.
authors.affiliation: Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Pfeiffer
authors.forename: A
authors.initials: A
authors.lastname: Böttcher
authors.forename: E
authors.initials: E
authors.lastname: Orsó
authors.forename: M
authors.initials: M
authors.lastname: Kapinsky
authors.forename: P
authors.initials: P
authors.lastname: Nagy
authors.forename: A
authors.initials: A
authors.lastname: Bodnár
authors.forename: I
authors.initials: I
authors.lastname: Spreitzer
authors.forename: G
authors.initials: G
authors.lastname: Liebisch
authors.forename: W
authors.initials: W
authors.lastname: Drobnik
authors.forename: K
authors.initials: K
authors.lastname: Gempel
authors.forename: M
authors.initials: M
authors.lastname: Horn
authors.forename: S
authors.initials: S
authors.lastname: Holmer
authors.forename: T
authors.initials: T
authors.lastname: Hartung
authors.forename: G
authors.initials: G
authors.lastname: Multhoff
authors.forename: G
authors.initials: G
authors.lastname: Schütz
authors.forename: H
authors.initials: H
authors.lastname: Schindler
authors.forename: A J
authors.initials: AJ
authors.lastname: Ulmer
authors.forename: H
authors.initials: H
authors.lastname: Heine
authors.forename: F
authors.initials: F
authors.lastname: Stelter
authors.forename: C
authors.initials: C
authors.lastname: Schütt
authors.forename: G
authors.initials: G
authors.lastname: Rothe
authors.forename: J
authors.initials: J
authors.lastname: Szöllôsi
authors.forename: S
authors.initials: S
authors.lastname: Damjanovich
authors.forename: G
authors.initials: G
authors.lastname: Schmitz
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D051928:CD47 Antigen; D002352:Carrier Proteins / Q000378:metabolism; D002518:Ceramides / Q000378:metabolism*; D029721:Drosophila Proteins; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008024:Ligands; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D021962:Membrane Microdomains / Q000378:metabolism*; D008565:Membrane Proteins; D009000:Monocytes / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D060225:Tetraspanin 28; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors
chemical_list: D015703:Antigens, CD; D051928:CD47 Antigen; C497894:CD47 protein, human; C554798:CD81 protein, human; D002352:Carrier Proteins; D002518:Ceramides; D029721:Drosophila Proteins; D008024:Ligands; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; C495345:TLR4 protein, human; D060225:Tetraspanin 28; D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors