Detection of a single copy gene on a mitotic metaphase chromosome by fluorescence in situ hybridization (FISH) in the sawfly, Athalia rosae (Hymenoptera).
Mitotic metaphase chromosomes of Athalia rosae (Hymenoptera) haploid males were subjected to fluorescence in situ hybridization (FISH) analysis using an rDNA probe and two vitellogenin (Vg) cDNA probes (one representing the 5' half and the other the 3' half of the gene, each about 3 kb long, and together covering the entire coding region). The rDNA probe produced signals in four chromosomes, all in pericentromeric regions (haploid chromosome number = 8), and the Vg probes, either the combined probes or the 3' region alone, produced a twin signal in the middle of a chromosome arm of a single chromosome. Arch.
Matsumoto
Kouhei
K
Division of Biology, Graduate School of Science and Technology, Kobe University, Nada, Kobe, Japan.
Yamamoto
Daisuke S
DS
Sumitani
Megumi
M
Lee
Jae Min
JM
Hatakeyama
Masatsugu
M
Oishi
Kugao
K
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods; D002875:Chromosomes / Q000235:genetics*; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D018628:Gene Dosage; D006927:Hymenoptera / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008677:Metaphase / Q000235:genetics; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics
D015342:DNA Probes; D018076:DNA, Complementary; D004275:DNA, Ribosomal; D014819:Vitellogenins