title: Co-localization of calretinin and calbindin in distinct cells in the hippocampal formation of the rat.
abstract: We studied the distribution of the calcium binding proteins calretinin and calbindin in the hippocampal formation of the rat brain by means of double-label immunofluorescence - confocal laser scanning microscopy. Colocalization of calretinin and calbindin occurred mostly in large neurons located in the alveus and stratum oriens of field CA1. Some double-labeled cells were observed in the transition area between field CA1 and the subiculum. Finally, double-labeled cells were present in the deep layer of the ventral subiculum. The cells in field CA1 co-expressing both proteins resemble neurons which in neurophysiological experiments by others have been identified as O-LM cells, and we believe that these co-expressing cells should be considered a distinct subpopulation of the calretinin and calbindin populations of GABAergic hippocampal interneurons.
authors.affiliation: Department of Anatomy, Research Institute for Neurosciences Vrije Universiteit, Vrije Universiteit Medical Center, 7 van der Boechorststraat, 1081 BT Amsterdam, The Netherlands. fg.wouterlood.anat@med.vu.nl
authors.forename: F G
authors.initials: FG
authors.lastname: Wouterlood
authors.forename: J
authors.initials: J
authors.lastname: Grosche
authors.forename: W
authors.initials: W
authors.lastname: Härtig
mesh_terms: D000818:Animals; D064032:Calbindin 2; D064026:Calbindins; D048429:Cell Size / Q000502:physiology; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure*; D005246:Feedback / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism*; D007395:Interneurons / Q000166:cytology* / Q000378:metabolism*; D018613:Microscopy, Confocal; D009433:Neural Inhibition / Q000502:physiology; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D051381:Rats; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid