Expression and control of C-type natriuretic peptide in rat vascular smooth muscle cells.
C-type natriuretic peptide (CNP) is a member of the natriuretic peptide family mainly distributed in the central nervous system. CNP is also produced and secreted by the endothelium and inhibits vascular smooth muscle cell proliferation. We have reported that endothelial damage stimulates only transiently vascular smooth muscle cell proliferation in arteries due to the development of an autocrine neointimal system for CNP that modulates neointimal growth. The present study demonstrates the production and secretion of CNP in rat vascular smooth muscle cells in the absence of endothelium. In addition, these cells express atrial natriuretic peptide (ANP) and the natriuretic peptide receptors A, B, and C. The production and secretion of CNP in vascular smooth muscle cells is stimulated by transforming growth factor-beta, whereas basic fibroblast growth factor plays an inhibitory role. These data show that ANP and mainly CNP are coexpressed with the natriuretic peptide receptors in rat vascular smooth muscle cells. This provides evidence for a vascular natriuretic peptide autocrine system of physiological relevance in these cells.
Woodard
Geoffrey E
GE
Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, United Kingdom. GeoffreyW@intra.niddk.nih.gov
Rosado
Juan A
JA
Brown
John
J
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001011:Aorta / Q000166:cytology; D009320:Atrial Natriuretic Factor / Q000235:genetics; D002478:Cells, Cultured; D002536:Cerebral Arteries / Q000166:cytology; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D020097:Natriuretic Peptide, Brain / Q000235:genetics; D020098:Natriuretic Peptide, C-Type / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000970:Antineoplastic Agents; D017931:DNA Primers; D007375:Interleukin-1; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D007371:Interferon-gamma; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor