Changes in predominance and diversity of genomic subtypes of Bordetella pertussis isolated in the United States, 1935 to 1999.
Pulsed-field gel electrophoresis (PFGE) of Bordetella pertussis chromosomal DNA fragments generated by XbaI restriction has been used to subtype isolates for epidemiologic studies. To better understand the natural history of pertussis, we determined the PFGE profiles of 1,333 strains isolated in the United States from 1935 to 1999. Results showed a shift in prevalent profiles from the earliest to the latest study periods. In addition, genetic diversity decreased over time, and prevalent profiles were more highly related to each other than to less common profiles. These results provide the foundation for investigating the impact of prevention strategies, including the use of the acellular vaccines, on the currently circulating B. pertussis population.
Hardwick
Terri Hawes
TH
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
Cassiday
Pamela
P
Weyant
Robbin S
RS
Bisgard
Kristine M
KM
Sanden
Gary N
GN
D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D010567:Pertussis Vaccine; D010802:Phylogeny; D015995:Prevalence; D015183:Restriction Mapping; D014481:United States / Q000453:epidemiology; D014917:Whooping Cough / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control
D004269:DNA, Bacterial; D010567:Pertussis Vaccine