title: Effect of genistein and quercetin on proliferation, collagen synthesis, and type I procollagen mRNA levels of rat hepatic stellate cells.
abstract: OBJECTIVE
  To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells.
  
  
  METHODS
  Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by [3H]proline incorporation assay. Type I mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  GE (25 - 70 micromol/L) and QU (6.25 - 50 micromol/L) concentration-dependently reduced serum-driven HSC-T6 cell proliferation and collagen synthesis associated with a suppression of type I procollagen mRNA level.
  
  
  CONCLUSIONS
  GE and QU inhibited hepatic stellate cell proliferation and collagen synthesis that might have a protective role against liver fibrosis.
authors.affiliation: Department of Pharmacy, Fuzhou General Hospital, Fuzhou 350001, China.
authors.forename: L P
authors.initials: LP
authors.lastname: Kang
authors.forename: L H
authors.initials: LH
authors.lastname: Qi
authors.forename: J P
authors.initials: JP
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Shi
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.forename: T M
authors.initials: TM
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Chen
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics; D019833:Genistein / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D024042:Collagen Type I; D006131:Growth Inhibitors; D012333:RNA, Messenger; D003094:Collagen; D011794:Quercetin; D019833:Genistein