title: Identification of genes encoding amino acid permeases by inactivation of selected ORFs from the Synechocystis genomic sequence.
abstract: Genes encoding elements of four amino acid permeases were identified by insertional inactivation of ORFs from the genomic sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 whose putative products are homologous to amino acid permease proteins from other bacteria. A transport system for neutral amino acids and histidine and a transport system for basic amino acids and glutamine were identified as ABC-type transporters, whereas Na(+)-dependent transport of glutamate was found to be mediated by at least two systems, the secondary permease GltS and a TRAP-type transporter. Except for GltS, substrate specificities of the identified permeases do not match those of previously characterized systems homologous to these permeases.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, E-41092 Seville, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Quintero
authors.forename: M L
authors.initials: ML
authors.lastname: Montesinos
authors.forename: A
authors.initials: A
authors.lastname: Herrero
authors.forename: E
authors.initials: E
authors.lastname: Flores
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics*; D026922:Amino Acid Transport Systems, Acidic / Q000235:genetics; D026923:Amino Acid Transport Systems, Basic / Q000235:genetics; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics; D020868:Gene Silencing; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D027981:Symporters / Q000235:genetics
chemical_list: D026905:Amino Acid Transport Systems; D026922:Amino Acid Transport Systems, Acidic; D026923:Amino Acid Transport Systems, Basic; D026921:Amino Acid Transport Systems, Neutral; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D027981:Symporters; C503373:glnP protein, E coli; C066603:gltS protein, E coli; C051432:glutamine permease