title: Synthesis and properties of bacteriorhodopsin analogs containing electron-density labels in the chromophore moiety.
abstract: A method for synthesis of retinal analogs labeled with electron-density groups is suggested. The interaction of these polyene compounds with bacterioopsin in apomembrane of Halobacterium salinarum was tested. A retinal analog containing a crown-ether receptor group is able to interact readily with bacterioopsin giving rise to rapid formation of a pigment with absorption maximum at 460 nm. This pigment is capable of undergoing cyclic photoconversion. The crown-bacteriorhodopsin photocycle is extremely slow and its quantum efficiency is very low (approximately 3% of that in native bacteriorhodopsin). This photocycle includes an M-like intermediate with a differential absorption maximum at 380 nm. A retinal analog in which the beta-ionone ring is replaced by ferrocene moiety forms a stable chromoprotein with the main absorption band at 483 nm and a shoulder near 590-610 nm.
authors.affiliation: Lomonosov State Academy of Fine Chemical Technology, Moscow, 117571, Russia. biotechnology@mtu-net.ru
authors.forename: E V
authors.initials: EV
authors.lastname: Mironova
authors.forename: A Y
authors.initials: AY
authors.lastname: Lukin
authors.forename: S V
authors.initials: SV
authors.lastname: Shevyakov
authors.forename: S G
authors.initials: SG
authors.lastname: Alexeeva
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
authors.forename: O V
authors.initials: OV
authors.lastname: Demina
authors.forename: A A
authors.initials: AA
authors.lastname: Khodonov
authors.forename: L V
authors.initials: LV
authors.lastname: Khitrina
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D004583:Electrons; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
chemical_list: D001436:Bacteriorhodopsins