Inhibitory effect of magnolol and honokiol from Magnolia obovata on human fibrosarcoma HT-1080. Invasiveness in vitro.
We investigated the inhibitory effect of Magnolia obovata Thunb. bark ethanol extracts on human fibrosarcoma HT-1080 cells invasion in a reconstituted basement membrane [Matrigel (MG)]. We found that the effective components of the bark ethanol extracts were magnolol and honokiol, two biphenyl compounds. The extracts, magnolol and honokiol, did not affect HT-1080 cells adhesion to MG, but did inhibit HT-1080 cells migration at a high concentration (100 microM). HT-1080 cells secrete matrix metalloproteinase (MMP)-9, which degrades the extracellular matrix as a part of the invasive process. Magnolol and honokiol inhibited the activity of MMP-9, which may have been responsible, in part, for the inhibition of tumor cell invasiveness.
Nagase
H
H
Department of Hygienics, Gifu Pharmaceutical University, Gifu, Japan. nagase@gifu-pu.ac.jp
Ikeda
K
K
Sakai
Y
Y
D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D001713:Biphenyl Compounds / Q000737:chemistry / Q000627:therapeutic use*; D005354:Fibrosarcoma / Q000188:drug therapy*; D006801:Humans; D017705:Lignans; D029562:Magnoliaceae / Q000737:chemistry; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D014407:Tumor Cells, Cultured; D000498:Allyl Compounds; D010636:Phenols
D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D017705:Lignans; D061965:Matrix Metalloproteinase Inhibitors; C005498:magnolol; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols