Isoforms of protein kinase C and their distribution in human adrenal cortex and tumors.
The cytosol and microsomal fractions of human adrenal cortex contain 3 isoforms of protein kinase C: alpha, zeta, and epsilon. The latter fraction is present in trace amounts. No isoforms beta1, beta2, gamma and delta were found in these cell fractions. The distribution of alpha-isoform between the cytosol and microsomal fraction is determined by tissue origin: in normal tissue its content differs by no more than 10%, while in most tumors this isoform is translocated into the microsomal fraction. The distribution of zeta-isoform did not depend on tissue origin.
Mikosha
A S
AS
V. P. Komissarenko Institute of Endocrinology and Metabolism, Academy of Medical Sciences of Ukraine, Kiev. endo@i.kiev.ua
Tron'ko
N D
ND
Staren'kii
D V
DV
Rybakov
S I
SI
D000302:Adrenal Cortex / Q000378:metabolism*; D000306:Adrenal Cortex Neoplasms / Q000737:chemistry* / Q000201:enzymology; D015153:Blotting, Western; D003600:Cytosol / Q000201:enzymology; D003720:Densitometry; D006801:Humans; D006965:Hyperplasia / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis; D008861:Microsomes / Q000201:enzymology; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000737:chemistry*; D051571:Protein Kinase C-alpha; D021381:Protein Transport; D014018:Tissue Distribution; D000099221:Protein Kinase C zeta
D007527:Isoenzymes; D020033:Protein Isoforms; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D000099221:Protein Kinase C zeta; C496628:PRKCA protein, human