title: Effect of RH-5992 on adult development in the spruce budworm, Choristoneura fumiferana.
abstract: The effect of RH-5992 (tebufenozide), a non-steroidal ecdysone agonist, on adult development of the spruce budworm, Choristoneura fumiferana, was investigated by administering the compound intrahemocoelically to pupae on days 1-6 after pupal ecdysis. At concentrations of 200ng/pupa there was significant mortality but at doses of 50-100ng/pupa, the emerging adults displayed wing deformities which reduced their ability to mate and oviposit. Light microscopy of the pupal wings revealed that there was degeneration of the epithelial cells, reduction in the number of veins, precocious cuticle formation and inhibition of growth of normal wing scales. Injection of RH-5992 into pupae resulted in a dose dependent induction of mRNA for ecdysone-induced transcription factor, Choristoneura hormone receptor 3 (CHR3). These results suggest that the pupae respond to RH-5992 in a manner similar to larvae. However, the effects are not expressed overtly and are camouflaged by the pharmacological effects.
authors.affiliation: Great Lakes Forestry Center, Canadian Forest Service, Natural Resources Canada, 1219 Queen St. E., Sault Ste. Marie, Ontario, Canada P6A E5.
authors.forename: M
authors.initials: M
authors.lastname: Sundaram
authors.forename: S R
authors.initials: SR
authors.lastname: Palli
authors.forename: G
authors.initials: G
authors.lastname: Smagghe
authors.forename: I
authors.initials: I
authors.lastname: Ishaaya
authors.forename: Q-L
authors.initials: QL
authors.lastname: Feng
authors.forename: M
authors.initials: M
authors.lastname: Primavera
authors.forename: W L
authors.initials: WL
authors.lastname: Tomkins
authors.forename: P J
authors.initials: PJ
authors.lastname: Krell
authors.forename: A
authors.initials: A
authors.lastname: Retnakaran
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D004440:Ecdysone / Q000819:agonists*; D006834:Hydrazines / Q000494:pharmacology*; D019476:Insect Proteins; D007605:Juvenile Hormones / Q000494:pharmacology*; D009036:Moths / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D018010:Receptors, Invertebrate Peptide / Q000235:genetics; D012098:Reproduction; D015534:Trans-Activators; D014921:Wings, Animal
chemical_list: C104701:CHR3 protein, Choristoneura fumiferana; D004268:DNA-Binding Proteins; D006834:Hydrazines; D019476:Insect Proteins; D007605:Juvenile Hormones; D012333:RNA, Messenger; D018010:Receptors, Invertebrate Peptide; D015534:Trans-Activators; D004440:Ecdysone; C082026:tebufenozide