title: Genes required for ionizing radiation resistance in yeast.
abstract: The ability of Saccharomyces cerevisiae to tolerate ionizing radiation damage requires many DNA-repair and checkpoint genes, most having human orthologs. A genome-wide screen of diploid mutants homozygous with respect to deletions of 3,670 nonessential genes revealed 107 new loci that influence gamma-ray sensitivity. Many affect replication, recombination and checkpoint functions. Nearly 90% were sensitive to other agents, and most new genes could be assigned to the following functional groups: chromatin remodeling, chromosome segregation, nuclear pore formation, transcription, Golgi/vacuolar activities, ubiquitin-mediated protein degradation, cytokinesis, mitochondrial activity and cell wall maintenance. Over 50% share homology with human genes, including 17 implicated in cancer, indicating that a large set of newly identified human genes may have related roles in the toleration of radiation damage.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Bennett
authors.forename: L K
authors.initials: LK
authors.lastname: Lewis
authors.forename: G
authors.initials: G
authors.lastname: Karthikeyan
authors.forename: K S
authors.initials: KS
authors.lastname: Lobachev
authors.forename: Y H
authors.initials: YH
authors.lastname: Jin
authors.forename: J F
authors.initials: JF
authors.lastname: Sterling
authors.forename: J R
authors.initials: JR
authors.lastname: Snipe
authors.forename: M A
authors.initials: MA
authors.lastname: Resnick
mesh_terms: D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D005720:Gamma Rays; D005800:Genes, Fungal; D009154:Mutation; D011003:Ploidies; D011836:Radiation Tolerance / Q000235:genetics*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects*
chemical_list: D017931:DNA Primers