title: Characterization of altered absorptive and secretory functions in the rat colon after abdominal irradiation: comparison with the effects of total-body irradiation.
abstract: The aim of this work was to determine the alterations in the absorptive and secretory functions of the rat colon after abdominal irradiation and to compare the effects of abdominal and whole-body irradiation. Rats received an abdominal irradiation with 8 to 12 Gy and were studied at 1, 4 and 7 days after exposure. Water and electrolyte absorption was measured in vivo by insertion of an agarose cylinder into the colons of anesthetized rats. In vitro measurements of potential difference, short-circuit current and tissue conductance were performed in Ussing chambers under basal and agonist-stimulated conditions. Most of the changes appeared at 4 days after abdominal irradiation. At this time, a decrease in water and electrolyte absorption in the colon was observed for radiation doses > or = 9 Gy. The response to secretagogues (VIP, 5-HT and forskolin) was attenuated after 10 and 12 Gy. Epithelial integrity, estimated by potential difference and tissue conductance, was altered from 1 to 7 days after 12 Gy abdominal irradiation. These results show that the function of the colon was affected by abdominal irradiation. Comparison with earlier results for total-body irradiation demonstrated a difference of 2 Gy in the radiation dose needed to induce changes in the function of the colon.
authors.affiliation: Institut de Protection et de Sûreté Nucléaire, Département de Protection de la santé de l'Homme et de Dosimétrie, IPSN, BP no. 6, F-92265 Fontenay-aux-Roses Cédex, France. isabelle.dublineau@ipsn.fr
authors.forename: I
authors.initials: I
authors.lastname: Dublineau
authors.forename: E
authors.initials: E
authors.lastname: Morel
authors.forename: N M
authors.initials: NM
authors.lastname: Griffiths
mesh_terms: D000005:Abdomen / Q000528:radiation effects*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003092:Colitis / Q000209:etiology* / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D004573:Electrolytes / Q000493:pharmacokinetics; D004847:Epithelial Cells / Q000528:radiation effects; D007408:Intestinal Absorption / Q000528:radiation effects*; D008297:Male; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D011836:Radiation Tolerance; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology; D014867:Water / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D004573:Electrolytes; D014867:Water; D005576:Colforsin; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D002217:Carbachol