Assessment of in vivo complement activation on the Echinococcus granulosus hydatid cyst wall.
The larval stage of the parasite Echinococcus granulosus causes hydatid disease. The hydatid cyst is potentially capable of activating host complement, since it is a large, persistent, carbohydrate-rich structure, coated with host immunoglobulins, and localized in the host's internal organs. Nonetheless, in vitro studies have suggested that the cyst surface, the hydatid cyst wall (HCW), is a poor complement activator. In this study, we assessed the occurrence of in vivo complement activation on the hydatid cyst by measuring the levels of two complement activation products, C3d and complexes bearing a C9 activation neoepitope (TCC/MAC), in extracts from HCW of human origin. Low amounts of C3d and TCC/MAC were found in HCW in comparison with their levels in normal human plasma and activated human sera, suggesting that in vivo complement activation on HCW is efficiently down-regulated. This regulation may contribute to limit host inflammation which has been observed to correlate with parasite degeneration and death.
Ferreira
A M
AM
Cátedra de Inmunología, Facultad de Ciencias/Facultad de Química, Universidad de la República, Montevideo, Uruguay. aferrei@bilbo.edu.uy
Diaz
A
A
Fernandez
C
C
Sim
R B
RB
D000818:Animals; D003167:Complement Activation; D015933:Complement C3d / Q000378:metabolism; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D004443:Echinococcosis / Q000276:immunology* / Q000469:parasitology; D004446:Echinococcus / Q000276:immunology*; D006801:Humans
D015938:Complement Membrane Attack Complex; D015933:Complement C3d