title: Experimental small bowel transplantation using a newborn intestine in rats: IV. Effect of cold preservation on graft neovascularization.
abstract: OBJECTIVE
  University of Wisconsin (UW) solution is one of the most superior organ preservation solutions for liver, kidney, and pancreas; however, it still is controversial for intestinal preservation. Here, the authors studied the efficacy of preservation with 2 kinds of solutions, UW and modified TOM (m-TOM) solutions in our experimental newborn intestinal transplantation model. UW solution was used as a standard intracellular and m-TOM solution as an extracellular preservation solution. Lactated ringer (LR) solution was used as a control.
  
  
  METHODS
  Newborn intestine, which were preserved in these solutions for 24 or 48 hours, were transplanted in the subcutaneous spaces of the syngeneic recipients without surgical vascular anastomosis and histologically examined 14 days after grafting. The preserved grafts were evaluated histologically by use of light and electron microscopy just after preservation. The biochemical parameters such as LDH and serotonin also were measured in the supernatants of preservation solutions.
  
  
  RESULTS
  Fresh newborn grafts were revascularized successfully at a rate of 80% (16 of 20). After 24 hours of preservation, 65% (13 of 20), 75% (15 of 20), and 85% (17 of 20) of the grafts were observed to be revascularized in LR, m-TOM, and UW solutions, respectively. After 48 hours of preservation, 60% (12 of 20), 80% (16 of 20), and 80% (16 of 20) of the grafts also were revascularized in the respective solutions (no statistic difference among the groups). The cold-preservation did not affect the neovascularization of newborn intestine until 48 hours. Histologic findings of the preserved intestine and biochemical analyses showed that UW and m-TOM solutions kept villous architectures of the preserved grafts, however, might be harmful to enterochromaffin cells.
  
  
  CONCLUSIONS
  Long-time preservation of newborn intestine did not interfere with neovascularization and maturation. J Pediatr Surg 36:1805-1810.
authors.affiliation: Division of Organ Replacement Research, Center for Molecular Medicine and Department of Anatomy, Jichi Medical School, Tochigi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Tahara
authors.forename: T
authors.initials: T
authors.lastname: Takizawa
authors.forename: K
authors.initials: K
authors.lastname: Inose
authors.forename: T
authors.initials: T
authors.lastname: Yashiro
authors.forename: K
authors.initials: K
authors.lastname: Hashizume
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006085:Graft Survival / Q000502:physiology; D007421:Intestine, Small / Q000033:anatomy & histology / Q000098:blood supply* / Q000637:transplantation*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D018919:Neovascularization, Physiologic; D019188:Organ Preservation Solutions / Q000737:chemistry / Q000592:standards*; D016377:Organ Transplantation / Q000379:methods* / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D012701:Serotonin / Q000032:analysis; D020858:Tissue and Organ Harvesting / Q000379:methods; D014185:Transplantation, Isogeneic
chemical_list: D019188:Organ Preservation Solutions; D012701:Serotonin; D007770:L-Lactate Dehydrogenase