AMPA-Induced suppression of oligodendroglial gene expression in neonatal rat brain.
Oligodendroglia are susceptible to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated excitotoxicity. We hypothesized that analysis of oligodendroglial gene expression would provide a sensitive indicator of acute excitotoxic oligodendroglial injury in the immature brain. AMPA (2 nmol) was injected intracerebroventricularly in 7-day-old rats. Proteolipid protein and myelin basic protein encoding mRNAs were evaluated 24-48 h later by semi-quantitative reverse transcription-polymerase chain reaction assays. AMPA markedly suppressed brain tissue content of oligodendroglial mRNAs.
Xu
H
H
Department of Pediatrics, University of Michigan, Room 8301 MSRB3, Box 0646, 1150 West Medical Center Drive, Ann Arbor, MI 48109-0646, USA.
Barks
J D
JD
Liu
Y Q
YQ
Silverstein
F S
FS
D000818:Animals; D000831:Animals, Newborn; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D007276:Injections, Intraventricular; D004676:Myelin Basic Protein / Q000235:genetics*; D018991:Myelin Proteolipid Protein / Q000235:genetics*; D009836:Oligodendroglia / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology*
D018690:Excitatory Amino Acid Agonists; D004676:Myelin Basic Protein; D018991:Myelin Proteolipid Protein; D012333:RNA, Messenger; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid