The extracellular domain of CD83 inhibits dendritic cell-mediated T cell stimulation and binds to a ligand on dendritic cells.
CD83 is an immunoglobulin (Ig) superfamily member that is upregulated during the maturation of dendritic cells (DCs). It has been widely used as a marker for mature DCs, but its function is still unknown. To approach its potential functional role, we have expressed the extracellular Ig domain of human CD83 (hCD83ext) as a soluble protein. Using this tool we could show that immature as well as mature DCs bind to CD83. Since CD83 binds a ligand also expressed on immature DCs, which do not express CD83, indicates that binding is not a homophilic interaction. In addition we demonstrate that hCD83ext interferes with DC maturation downmodulating the expression of CD80 and CD83, while no phenotypical effects were observed on T cells. Finally, we show that hCD83ext inhibits DC-dependent allogeneic and peptide-specific T cell proliferation in a concentration dependent manner in vitro. This is the first report regarding functional aspects of CD83 and the binding of CD83 to DCs.
Lechmann
M
M
Department of Dermatology, University of Erlangen-Nuremberg, D-91052 Erlangen, Germany.
Krooshoop
D J
DJ
Dudziak
D
D
Kremmer
E
E
Kuhnt
C
C
Figdor
C G
CG
Schuler
G
G
Steinkasserer
A
A
D017951:Antigen Presentation; D015703:Antigens, CD; D002450:Cell Communication / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D000097802:CD83 Antigen
D015703:Antigens, CD; D007128:Immunoglobulin Fragments; D007136:Immunoglobulins; D008024:Ligands; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen