Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5'-W (downward arrow)GTACW-3' and 5'-GCSG (downward arrow)C-3' respectively.
One hundred and forty isolates of thermophilic bacteria from the genus Thermus were screened for the presence of restriction endonuclease activity. Thermostable isoschizomers of restriction endonucleases, such as AceIII, BbvI, BglI, BsePI, FnuDII, HgiAI, MaeII, MboI, MseI, PvuII, StuI, TaqI, Tsp4CI, TspEI, XhoI and XmaIII, were isolated. Two restriction enzymes, TatI and TauI, recognizing novel degenerate sequences 5'-W (downward arrow)GTACW-3' and 5'-GCSG (downward arrow)C-3' respectively were partially purified and the recognition and cleavage sites were determined.
Vitkute
J
J
Institute of Biotechnology, Graiciūno 8, Vilnius, Lithuania. janulait@ibt.lt
Maneliene
Z
Z
Janulaitis
A
A
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D013379:Substrate Specificity; D013824:Thermus / Q000201:enzymology*
D004247:DNA; C445676:endodeoxyribonuclease TatI; C445677:endodeoxyribonuclease TauI; D015252:Deoxyribonucleases, Type II Site-Specific