title: Comparison of three chiral stationary phases with respect to their enantio- and diastereoselectivity for cyclic beta-substituted alpha-amino acids.
abstract: Three chiral stationary phases were examined for the enantio- and diastereoseparation of cycloaliphatic beta-substituted alpha-quaternary alpha-amino acids. Resolution of diastereomeric analytes is feasible with a chiral crown ether based column, whereas the separation of enantiomers, except for one pair of amino acids, could not be achieved. The two chiral stationary phases with the glycopeptide antibiotic teicoplanin and with the copper(II)-D-penicillamine complex, respectively, are, however, both very potent in the separation of the enantiomeric, as well as of the diastereomeric amino acids. A baseline separation of all four stereoisomeric forms in one chromatographic run was possible with the exception of one type of amino acid. The results of the method development are presented in this paper.
authors.affiliation: Department of Pharmaceutical Chemistry, Albert-Ludwigs-University, Albertstr. 25, D-79104 Freiburg im Breisgau, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Schlauch
authors.forename: Olha
authors.initials: O
authors.lastname: Kos
authors.forename: August W
authors.initials: AW
authors.lastname: Frahm
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids