Effect of Mangifera indica L. extract (QF808) on protein and hepatic microsome peroxidation.
The antioxidant activities of QF808, a steam bark extract of Mangifera indica L., were studied on hydroxyl-mediated oxidation of bovine serum albumin (BSA) and in a hepatic microsome system. The extract was effective in reducing the oxidation of BSA, since its half- maximal inhibition concentration (IC(50)) was 0.0049% w/v in the inhibition of carbonyl group formation and lower than 0.0025% w/v in the inhibition of sulfhydryl group loss. QF808 inhibited lipid peroxidation which was initiated enzymatically by reduced nicotinamide adenine dinucleotide phosphate (NADPH), IC(50)= 0.00075% w/v, or non-enzymatically by ascorbic acid, IC(50) = 0.0126% w/v. The extract tested did not inhibit NADPH-dependent cytochrome P-450 reductase activity, since it had no effect on the oxidation rate of NADPH. These results suggest that QF808 has an antioxidant activity, probably due to its ability to scavenge free radicals involved in microsome lipid peroxidation. In addition, QF808 antioxidant profile in vitro is probably similar to its principal polyphenolic component, mangiferin, a glycosylated xanthone.
Martínez
G
G
Center for Evaluation and Biological Research. Institute of Pharmacy, Havana University, San Lázaro y L, Havana 4, Cuba. gregorio@cieb.sld.cu
Giuliani
A
A
León
O S
OS
Pérez
G
G
Núñez Selles
A J
AJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects; D005260:Female; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects*; D008862:Microsomes, Liver / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley; D029631:Sapindaceae; D012710:Serum Albumin, Bovine / Q000187:drug effects*
D000975:Antioxidants; D010936:Plant Extracts; D012710:Serum Albumin, Bovine; D003577:Cytochrome P-450 Enzyme System