Retroviral activity in Behçet's syndrome and systemic lupus erythematosus detected by a PCR-based reverse transcriptase assay.
The objective of this study was to assess retroviral activity in Behçet's syndrome (BS) and systemic lupus erythematosus (SLE) patients. Serum and peripheral blood mononuclear cells (PBMC) were obtained from patients and normal volunteers and PBMC cultured with and without phytohaemagglutinin stimulation. Reverse transcriptase (RT) activity in serum and culture supernatants was measured using a sensitive polymerase chain reaction-based assay. An RT activity above the levels in normal controls was detected in a minority of patients with BS (2/15) and SLE (1/13) and was typically present in all three types of sample. Elevated levels of RT activity were not detected in follow-up samples from the two BS patients. Our findings indicate that elevated RT activity is present in only a minority of patients with BS and SLE. Simultaneously elevated activity in all three sample types implicates PBMC as the source of this retroviral activity.
McIntosh
R S
RS
Division of Immunology, University of Nottingham, Queen's Medical Centre, UK. richard.mcintosh@nottingham.ac.uk
Huggins
M L
ML
Kontogiannis
V
V
Tighe
P J
PJ
Powell
R J
RJ
D000328:Adult; D001528:Behcet Syndrome / Q000097:blood / Q000821:virology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000821:virology; D012016:Reference Values; D012190:Retroviridae / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012494:Sampling Studies; D012680:Sensitivity and Specificity