Expression of IL-17B in neurons and evaluation of its possible role in the chromosome 5q-linked form of Charcot-Marie-Tooth disease.
IL-17B is a recently identified homolog of IL-17. Northern analysis revealed that IL-17B mRNA is expressed at very high levels in spinal cord and at much lower and more variable levels in trachea, prostate, lung, small intestine, testes, adrenal, and pancreas. In developing mouse embryos IL-17B expression was first detected at day 11 and appeared to peak at day 15. In situ analysis of mouse spinal cord, dorsal root ganglia, and brain demonstrated that IL-17B mRNA is primarily expressed by the neurons. Immunohistochemical analysis of human spinal cord, dorsal root ganglia, cerebral cortex, cerebellum, and hippocampus demonstrated that IL-17B protein is primarily localized to the neuronal cell bodies and axons. Radiation hybrid mapping localized the IL-17B gene to a region on human chromosome 5q that is associated with a rare autosomal recessive form of Charcot-Marie-Tooth demyelinating disease. However, no changes were found in the coding regions, splice junctions, intron 1, or the 5' and 3' untranslated regions of IL-17B genes of patients affected with this disease.
Moore
Emma E
EE
ZymoGenetics Inc, 1201 Eastlake Avenue East, Seattle, WA 98102, USA. mooreb@zgi.com
Presnell
Scott
S
Garrigues
Ursula
U
Guilbot
Angele
A
LeGuern
Eric
E
Smith
Deborah
D
Yao
Lena
L
Whitmore
Theodore E
TE
Gilbert
Teresa
T
Palmer
Theo D
TD
Horner
Philip J
PJ
Kuestner
Rolf E
RE
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000276:immunology; D002460:Cell Line; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D005314:Embryonic and Fetal Development; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015723:Gene Library; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009474:Neurons / Q000276:immunology* / Q000502:physiology*; D009928:Organ Specificity; D011467:Prostate / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000276:immunology; D014132:Trachea / Q000378:metabolism; D014158:Transcription, Genetic
D020381:Interleukin-17; D012333:RNA, Messenger