An updated linkage and comparative map of porcine chromosome 18.
Swine chromosome 18 (SSC18) has the poorest marker density in the USDA-MARC porcine linkage map. In order to increase the marker density, seven genes from human chromosome 7 (HSA7) expected to map to SSC18 were selected for marker development. The genes selected were: growth hormone releasing hormone receptor (GHRHR), GLI-Kruppel family member (GLI3), leptin (LEP), capping protein muscle Z-line alpha 2 subunit (CAPZA2), beta A inhibin (INHBA), T-cell receptor beta (TCRB) and T-cell receptor gamma (TCRG). Large-insert clones (YACs, BACs and cosmids) that contained these genes, as well as two previously mapped microsatellite markers (SW1808 and SW1984), were identified and screened for microsatellites. New microsatellite markers were developed from these clones and mapped. Selected clones were also physically assigned by fluorescence in situ hybridization (FISH). Fifteen new microsatellite markers were added to the SSC18 linkage map resulting in a map of 28 markers. Six genes have been included into the genetic map improving the resolution of the SSC18 and HSA7 comparative map. Assignment of TCRG to SSC9 has identified a break in conserved synteny between SSC18 and HSA7.
Campbell
E M
EM
USDA, ARS, US Meat Animal Research Center, PO Box 166, Spur 18D, Clay Center, NE 68933-0166, USA.
Fahrenkrug
S C
SC
Vallet
J L
JL
Smith
T P
TP
Rohrer
G A
GA
D000818:Animals; D051347:CapZ Actin Capping Protein; D002874:Chromosome Mapping / Q000662:veterinary*; D002897:Chromosomes, Human, Pair 7; D004268:DNA-Binding Proteins / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D028322:Inhibin-beta Subunits / Q000235:genetics; D051741:Kruppel-Like Transcription Factors; D020738:Leptin / Q000235:genetics; D008840:Microfilament Proteins / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D012097:Repressor Proteins; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D029867:Xenopus Proteins; D000074290:Zinc Finger Protein Gli3
D051347:CapZ Actin Capping Protein; D004268:DNA-Binding Proteins; C108003:GLI3 protein, Xenopus; C065683:GLI3 protein, human; C465571:Gli3 protein, mouse; D051741:Kruppel-Like Transcription Factors; D020738:Leptin; D008840:Microfilament Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012097:Repressor Proteins; D014157:Transcription Factors; D029867:Xenopus Proteins; D000074290:Zinc Finger Protein Gli3; C103008:inhibin beta A subunit; D028322:Inhibin-beta Subunits