Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath).
The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A DeltahupSL deletion mutant of Mc. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo.
Csáki
R
R
Department of Biotechnology, University of Szeged, Hungary.
Hanczár
T
T
Bodrossy
L
L
Murrell
J C
JC
Kovács
K L
KL
D001426:Bacterial Proteins; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism; D020619:Methylococcus capsulatus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D006864:Hydrogenase