title: Physiological migration of hematopoietic stem and progenitor cells.
abstract: Hematopoietic stem cells (HSCs) reside predominantly in bone marrow, but low numbers of HSCs are also found in peripheral blood. We examined the fate of blood-borne HSCs using genetically marked parabiotic mice, which are surgically conjoined and share a common circulation. Parabionts rapidly established stable, functional cross engraftment of partner-derived HSCs and maintained partner-derived hematopoiesis after surgical separation. Determination of the residence time of injected blood-borne progenitor cells suggests that circulating HSCs/progenitors are cleared quickly from the blood. These data demonstrate that HSCs rapidly and constitutively migrate through the blood and play a physiological role in, at least, the functional reengraftment of unconditioned bone marrow.
authors.affiliation: Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Wright
authors.forename: A J
authors.initials: AJ
authors.lastname: Wagers
authors.forename: A P
authors.initials: AP
authors.lastname: Gulati
authors.forename: F L
authors.initials: FL
authors.lastname: Johnson
authors.forename: I L
authors.initials: IL
authors.lastname: Weissman
mesh_terms: D000818:Animals; D001775:Blood Circulation / Q000502:physiology; D002465:Cell Movement; D002678:Chimera / Q000097:blood*; D006410:Hematopoiesis / Q000502:physiology*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006706:Homeostasis; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D013997:Time Factors
chemical_list: D017493:Leukocyte Common Antigens