title: p120(cat) Delocalization in cell lines of oral cancer.
abstract: p120(cat) is a novel component of the catenin family, a cytoplasmic molecule closely associated with the cell-cell adhesion molecule E (epithelial)-cadherin, by forming complexes between the cytoplasmic domain of E-cadherin and the cytoskeleton. Recent studies suppose a role for this molecule in human cancers and to date none report its expression in oral squamous cell carcinomas (SCCs). The goal of this study was to evaluate the role of this protein in the oral carcinogenetic process. A linked streptavidin-biotin-alkaline phosphatase technique was used to examine the immunoreactivity and cellular localisation of p120(cat) in five oral epithelial cell lines (NCTC 2544, normal and immortalized keratinocytes; KB, a poorly differentiated SCC cell line; OSC 20, a well differentiated oral SCC cell line; CAL 33 and CAL 27, moderately differentiated oral SCC cell lines) and 10 normal oral epithelium biopsies.
  
  
  RESULTS
  As already reported for E-cadherin, beta- and gamma-catenin, p120 expression showed a homogeneous membranous localization in normal oral specimens. The intensity of staining for p120 progressively increased from basal and parabasal layers toward the intermediate spinous layer. No staining for p120 was observed in the upper layer. NCTC showed a membranous positivity. OSC 20, CAL 33 and CAL 27 showed a membranous positivity, even if polarized to cell-cell adhesion sites, in 40-50% of cells. OSC 20, CAL 33 and CAL 27 cells showed also a cytoplasmic delocalization. All positive KB cells showed a prevalent cytoplasmic staining and 10% of these cells showed a nuclear delocalization. In cancer cells, p120 showed an inverse relationship with the degree of differentiation for a progressive displacement of the signal toward the cytoplasm or nucleus in dedifferentiated cells. In conclusions, this nuclear delocalization for p120 could suppose its potential involvement in signalling and cancer transformation.
authors.affiliation: Institute of Dental Sciences, University of Ancona, Ancona, Italy. llomuzio@tin.it
authors.forename: L
authors.initials: L
authors.lastname: Lo Muzio
authors.forename: G
authors.initials: G
authors.lastname: Pannone
authors.forename: S
authors.initials: S
authors.lastname: Staibano
authors.forename: M D
authors.initials: MD
authors.lastname: Mignogna
authors.forename: R
authors.initials: R
authors.lastname: Serpico
authors.forename: S
authors.initials: S
authors.lastname: Fanali
authors.forename: G
authors.initials: G
authors.lastname: De Rosa
authors.forename: A
authors.initials: A
authors.lastname: Piattelli
authors.forename: M A
authors.initials: MA
authors.lastname: Mariggiò
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D009062:Mouth Neoplasms / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D000095002:Delta Catenin
chemical_list: D051177:Catenins; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D000095002:Delta Catenin