title: Effect of quercetin on adhesion of platelets to microvascular endothelial cells in vitro.
abstract: OBJECTIVE
  To study the effect of quercetin(Que) on the adhesion of platelets to microvascular endothelial cells (MVEC) isolated from human skin.
  
  
  METHODS
  [3H]-adenine-labeled platelets were incubated with MVEC. Effect of Que on platelet endothelial cell adhesion molecular (PECAM) expression on MVEC was also evaluated using enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Adhesion of platelet to MVEC reached to maximum at about 30 min. Que inhibited the adhesion of platelets to MVEC in a concentration-dependent manner. Que 5 micromol/L did not show any significant inhibition. When the concentration of Que increased to 10, 20, and 40 micromol/L, the inhibition rate increased to 10.5 %, 20.0 %, and 42.2 %, respectively. Pre-incubation of Que (10 - 40 micromol/L) with labeled platelets for 30 min also inhibited the adhesion but Que 5 micromol/L did not. The inhibition rate of Que 10, 20, and 40 micromol/L was 18.2 %, 29.8 %, and 65.3 % respectively. Expression of PECAM on the endothelial cells was decreased in a concentration-dependent manner when MVEC were treated with Que (10 - 40 micromol/L) for 12 h but Que 5 micromol/L did not significantly affect the expression.
  
  
  CONCLUSIONS
  Que could inhibit the adhesion of platelets to MVEC. This effect may be related to decreased expression of PECAM on MVEC.
authors.affiliation: Department of Pharmacology, Suzhou University, Suzhou Institute of Chinese Materia Medica, Suzhou 215007, China.
authors.forename: P S
authors.initials: PS
authors.lastname: Fan
authors.forename: Z L
authors.initials: ZL
authors.lastname: Gu
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Liang
mesh_terms: D001798:Blood Proteins / Q000494:pharmacology*; D002196:Capillaries / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D001798:Blood Proteins; C024875:platelet adhesion inhibitor; D011794:Quercetin