Vitamin E ameliorates aflatoxin-induced biochemical changes in the testis of mice.
OBJECTIVE
To assess the effect of aflatoxin on biochemical changes in the testis of mice and the possibility of amelioration by vitamin E treatment.


METHODS
Adult male albino mice were orally administered with 25 or 50 microg of aflatoxin/animal/day (750 or 1500 microg/kg body weight) for 45 days. The testis was isolated and processed for biochemical analysis.


RESULTS
There was a significant, dose-dependent reduction in DNA, RNA, protein, sialic acid contents and the activities of succinic dehydrogenase, adenosine triphosphatase and alkaline phosphatase in the testis of aflatoxin-treated mice as compared to the vehicle control. However, the acid phosphatase activity was significantly increased in the aflatoxin-treated mice. Vitamin E (2 mg/animal/day) treatment significantly ameliorated the aflatoxin-induced changes, except the acid and alkaline phosphatase activities in the high dose group.


CONCLUSIONS
Vitamin E treatment ameliorates the aflatoxin-induced changes in the testis of mice.
Verma
R J
RJ
Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad, India. zooldeptgu@satyam.net.in
Nair
A
A
D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000348:Aflatoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D008297:Male; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
D000348:Aflatoxins; D000975:Antioxidants; D011506:Proteins; D014810:Vitamin E; D012313:RNA; D004247:DNA; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases; D019158:N-Acetylneuraminic Acid