title: Characterization of vibrissa germinative cells: transition of cell types.
abstract: Germinative cells, small cell masses attached to the stalks of dermal papillae that are able to differentiate into the hair shaft and inner root sheath, form follicular bulb-like structures when co-cultured with dermal papilla cells. We studied the growth characteristics of germinative cells to determine the cell types in the vibrissa germinative tissue. Germinative tissues, attaching to dermal papillae, were cultured on 3T3 feeder layers. The cultured keratinocytes were harvested and transferred, equally and for two passages, onto lined dermal papilla cells (LDPC) and/or 3T3 feeder layers. The resulting germinative cells were classified into three types in the present experimental condition. Type 1 cells grow very well on either feeder layer, whereas Type 3 cells scarcely grow on either feeder layer. Type 2 cells are very conspicuous and are reversible. They grow well on 3T3 but growth is suppressed on LDPC feeder layers. The Type 2 cells that grow well on 3T3 feeder layers, however, are suppressed when transferred onto LDPC and the Type 2 cells that are suppressed on LDPC begin to grow again on 3T3. The transition of one cell type to another in vitro and the cell types that these germinative cell types correspond to in vivo is discussed. It was concluded that stem cells or their close progenitors reside in the germinative tissues of the vibrissa bulb except at late anagen-early catagen.
authors.affiliation: Graduate School of Science and Technology, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Osada
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002477:Cells / Q000145:classification; D002478:Cells, Cultured; D002999:Clone Cells; D018920:Coculture Techniques; D018859:Hair Follicle / Q000166:cytology*; D015603:Keratinocytes / Q000166:cytology*; D051379:Mice; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D014738:Vibrissae / Q000166:cytology* / Q000502:physiology