title: Predominant IL-10 production in indirect alloreactivity is not associated with rejection.
abstract: In a prospective study of indirect alloresponse in renal transplantation, we detected proliferation and cytokine production to donor and third-party HLA-DR peptides unrelated to rejection. Twenty of 28 patients (71%) presented proliferation, 29% before and 71% after transplantation. Half of the patients also presented proliferation to third-party peptides. Indirect alloresponse was also detected in 75% of healthy individuals (HI). Variability of response was observed in patients and HI for both proliferation and cytokine production. IL-10 predominance was observed in indirect alloresponses to donor peptides pre- and post-Tx, in contrast with more IFN-gamma and TGF-beta being detected in HI. IL-10 production was frequently detected without proliferation, in contrast with more frequent proliferation being found with IFN-gamma and TGF-beta production. The lack of association of either cytokine or proliferation with rejection, together with the predominance of IL-10 unrelated to proliferation, suggests that regulatory cells may be part of the T cell repertoire involved in indirect alloreactivity.
authors.affiliation: Heart Institute-InCor, University of São Paulo, Medical School, 05403-000, São Paulo, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Spadafora-Ferreira
authors.forename: J A
authors.initials: JA
authors.lastname: Fonseca
authors.forename: C
authors.initials: C
authors.lastname: Granja
authors.forename: D M
authors.initials: DM
authors.lastname: Malheiros
authors.forename: J
authors.initials: J
authors.lastname: Kalil
authors.forename: V
authors.initials: V
authors.lastname: Coelho
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D006084:Graft Rejection / Q000276:immunology*; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D016030:Kidney Transplantation; D008969:Molecular Sequence Data; D011446:Prospective Studies; D016212:Transforming Growth Factor beta / Q000276:immunology; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
chemical_list: D006684:HLA-DR Antigens; D007519:Isoantigens; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma