Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10.
The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immunoprecipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.
Maruyama
S
S
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan.
Hatakeyama
S
S
Nakayama
K
K
Ishida
N
N
Kawakami
K
K
Nakayama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D044783:F-Box Proteins; D015870:Gene Expression; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D000073979:F-Box-WD Repeat-Containing Protein 7
D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D044783:F-Box Proteins; D015801:Helminth Proteins; C109554:SEL-10 protein, C elegans; C513272:Fbxw7 protein, mouse; D000073979:F-Box-WD Repeat-Containing Protein 7