Remodeling of synaptic actin induced by photoconductive stimulation.
Use-dependent synapse remodeling is thought to provide a cellular mechanism for encoding durable memories, yet whether activity triggers an actual structural change has remained controversial. We use photoconductive stimulation to demonstrate activity-dependent morphological synaptic plasticity by video imaging of GFP-actin at individual synapses. A single tetanus transiently moves presynaptic actin toward and postsynaptic actin away from the synaptic junction. Repetitive spaced tetani induce glutamate receptor-dependent stable restructuring of synapses. Presynaptic actin redistributes and forms new puncta that label for an active synapse marker FM5-95 within 2 hr. Postsynaptic actin sprouts projections toward the new presynaptic actin puncta, resembling the axon-dendrite interaction during synaptogenesis. Our results indicate that activity-dependent presynaptic structural plasticity facilitates the formation of new active presynaptic terminals.
Colicos
M A
MA
Neurobiology Section/Division of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Collins
B E
BE
Sailor
M J
MJ
Goda
Y
Y
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000199:Actins / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000166:cytology; D008027:Light; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018715:Microscopy, Video; D009473:Neuronal Plasticity / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D012825:Silicon / Q000737:chemistry; D013569:Synapses / Q000378:metabolism*; D013873:Thionucleotides / Q000494:pharmacology; D014633:Valine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism
D000199:Actins; D000814:Aniline Compounds; D004791:Enzyme Inhibitors; D018691:Excitatory Amino Acid Antagonists; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; D013873:Thionucleotides; D014966:Xanthenes; D049452:Green Fluorescent Proteins; C016957:adenosine-3',5'-cyclic phosphorothioate; C059715:Fluo-3; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; C056639:2-amino-5-phosphopentanoic acid; D000242:Cyclic AMP; D014633:Valine; D002118:Calcium; D012825:Silicon