Effect of quercetin on adhesion of platelets to microvascular endothelial cells in vitro.
OBJECTIVE
To study the effect of quercetin(Que) on the adhesion of platelets to microvascular endothelial cells (MVEC) isolated from human skin.


METHODS
[3H]-adenine-labeled platelets were incubated with MVEC. Effect of Que on platelet endothelial cell adhesion molecular (PECAM) expression on MVEC was also evaluated using enzyme-linked immunosorbent assay (ELISA).


RESULTS
Adhesion of platelet to MVEC reached to maximum at about 30 min. Que inhibited the adhesion of platelets to MVEC in a concentration-dependent manner. Que 5 micromol/L did not show any significant inhibition. When the concentration of Que increased to 10, 20, and 40 micromol/L, the inhibition rate increased to 10.5 %, 20.0 %, and 42.2 %, respectively. Pre-incubation of Que (10 - 40 micromol/L) with labeled platelets for 30 min also inhibited the adhesion but Que 5 micromol/L did not. The inhibition rate of Que 10, 20, and 40 micromol/L was 18.2 %, 29.8 %, and 65.3 % respectively. Expression of PECAM on the endothelial cells was decreased in a concentration-dependent manner when MVEC were treated with Que (10 - 40 micromol/L) for 12 h but Que 5 micromol/L did not significantly affect the expression.


CONCLUSIONS
Que could inhibit the adhesion of platelets to MVEC. This effect may be related to decreased expression of PECAM on MVEC.
Fan
P S
PS
Department of Pharmacology, Suzhou University, Suzhou Institute of Chinese Materia Medica, Suzhou 215007, China.
Gu
Z L
ZL
Liang
Z Q
ZQ
D001798:Blood Proteins / Q000494:pharmacology*; D002196:Capillaries / Q000166:cytology; D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011794:Quercetin / Q000494:pharmacology*
D001798:Blood Proteins; C024875:platelet adhesion inhibitor; D011794:Quercetin