Messenger RNAs in synaptosomal fractions from rat brain.
Synaptosomal fractions from rat brain have been analyzed with semi-quantitative RT-PCR methods to determine their content of mRNAs coding for presynaptic, postsynaptic, glial, and neuronal proteins. Each mRNA was determined with reference to the standard HPRT mRNA. In our analyses, mRNAs were considered to be associated with synaptosomes only if their relative amounts were higher than in microsomes prepared in a polysome stabilizing medium, rich in Mg(++) and K(+) ions, or in the homogenate. According to this stringent criterion, the following synaptosomal mRNAs could not be attributed to microsomal contamination and were assumed to derive from the subcellular structures known to harbor their translation products, i.e. GAT-1 mRNAs from presynaptic terminals and glial processes, MAP2 mRNA from dendrites, GFAP mRNA from glial processes, and TAU mRNA from neuronal fragments. This interpretation is in agreement with the involvement of extrasomatic mRNAs in local translation processes.
Crispino
M
M
Dipartimento di Fisiologia Generale e Ambientale, Università di Napoli Federico II, Naples, Italy.
Capano
C P
CP
Aiello
A
A
Iannetti
E
E
Cupello
A
A
Giuditta
A
A
D000818:Animals; D001921:Brain / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D008297:Male; D008861:Microsomes / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics; D009457:Neuroglia / Q000502:physiology; D009474:Neurons / Q000502:physiology; D017729:Presynaptic Terminals / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions; D013574:Synaptosomes / Q000502:physiology*
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger