Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport.
A cisternal progression mode of intra-Golgi transport requires that Golgi resident proteins recycle by peri-Golgi vesicles, whereas the alternative model of vesicular transport predicts anterograde cargo proteins to be present in such vesicles. We have used quantitative immuno-EM on NRK cells to distinguish peri-Golgi vesicles from other vesicles in the Golgi region. We found significant levels of the Golgi resident enzyme mannosidase II and the transport machinery proteins giantin, KDEL-receptor, and rBet1 in coatomer protein I-coated cisternal rims and peri-Golgi vesicles. By contrast, when cells expressed vesicular stomatitis virus protein G this anterograde marker was largely absent from the peri-Golgi vesicles. These data suggest a role of peri-Golgi vesicles in recycling of Golgi residents, rather than an important role in anterograde transport.
Martinez-Menárguez
J A
JA
Department of Cell Biology, School of Medicine, University of Murcia, 30071 Murcia, Spain.
Prekeris
R
R
Oorschot
V M
VM
Scheller
R
R
Slot
J W
JW
Geuze
H J
HJ
Klumperman
J
J
D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D020755:Coat Protein Complex I; D006056:Golgi Apparatus / Q000502:physiology* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D049452:Green Fluorescent Proteins; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D007668:Kidney; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D021381:Protein Transport; D051381:Rats; D018000:Receptors, Peptide / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000378:metabolism
D001324:Autoantigens; D020755:Coat Protein Complex I; C028391:G protein, vesicular stomatitis virus; D000077614:Golgi Matrix Proteins; C064459:KDEL receptor; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018000:Receptors, Peptide; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C087574:macrogolgin; D049452:Green Fluorescent Proteins