Validation of a single nucleotide polymorphism genotyping assay for the human serum paraoxonase gene using electronically active customized microarrays.
OBJECTIVE
Develop a microarray based genotyping assay to detect SNPs in human paraoxonase (PON I) and compare its accuracy with DNA sequencing and RFLP based assays.


METHODS
Amplicons spanning the polymorphic regions of interest were genotyped by sequencing, RFLP, and microarray technology. Validation parameters included precision, linearity of signal response, and carryover between adjacent sites on the microarray.


RESULTS
A 100% correlation in results obtained using DNA sequencing, RFLP and microarray technology was observed.


CONCLUSIONS
The microarray technology provides an accurate and reliable assay platform with applicability in high throughput genotyping studies.
Nagan
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Division of Clinical Biochemistry and Immunology, Department of Laboratory Medicine and Pathology, Mayo Foundation and Clinic, 200 First Street SW, Rochester, MN 55905, USA.
O'Kane
D J
DJ
D043303:Aryldialkylphosphatase; D004950:Esterases / Q000097:blood / Q000235:genetics*; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results
D004950:Esterases; D043303:Aryldialkylphosphatase