title: Protection of the lungs from acid during aspiration.
abstract: Unlike the thick mucosa that normally covers the upper gastrointestinal tract, the membranes that cover the distal surfaces of the lungs are remarkably attenuated. This permits rapid exchange of gases between the airspaces and pulmonary vasculature, and may make the lungs more susceptible to acid challenges associated with acid reflux and aspiration. Any injury to the alveolar epithelium could result in the movement of solute and water into the airspaces (chemical pneumonia) and impair gas exchange. In this study, we used a fluorescent approach to compare the relative permeability of the apical basolateral surfaces of the lungs to the exchange of the ionic forms of acids and bases. The apical membranes proved to be much less permeable to NH(4)(+) and HCO(3)(+) than the basolateral membranes. This asymmetry in permeability should enhance resistance of the epithelium to inspired acidic challenges by slowing entry of acid into the cells and by linking the intracellular pH of the alveolar cells to that of the plasma, which is a relatively large, well-buffered compartment. Evidence also was obtained that the acid is secreted by the membranes covering the lungs.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Medical College of Wisconsin, 9200 W. Wisconsin Avenue, Milwaukee, WI 53226, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Effros
authors.forename: G
authors.initials: G
authors.lastname: Hogan
authors.forename: K
authors.initials: K
authors.lastname: Wahlen Hoagland
authors.forename: L
authors.initials: L
authors.lastname: Olson
authors.forename: W
authors.initials: W
authors.lastname: Lin
mesh_terms: D000643:Ammonium Chloride / Q000494:pharmacology*; D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology; D046508:Culture Techniques; D005453:Fluorescence; D005744:Gastric Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D001239:Inhalation / Q000502:physiology*; D008168:Lung / Q000187:drug effects* / Q000502:physiology*; D051381:Rats; D012016:Reference Values
chemical_list: D000643:Ammonium Chloride