title: 14-3-3 Proteins are essential for regulation of RTG3-dependent transcription in Saccharomyces cerevisiae.
abstract: 14-3-3 proteins comprise a family of highly conserved proteins that bind more than 60 different, mostly phosphorylated, proteins. The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2, encoding 14-3-3 proteins. Disruption of both genes together is lethal. In this study we constructed a mutant with a single, temperature-sensitive bmh allele. Recessive mutations in SIN4 and RTG3 can suppress the temperature-sensitive phenotype of this mutant. These genes encode a global transcriptional regulator and a basic helix-loop-helix transcription factor, respectively. The yeast 14-3-3 proteins were shown to bind to the Rtg3 protein. Overexpression of RTG3 is lethal even in wild-type cells. These genetic and biochemical data are consistent with a model in which the 14-3-3 proteins are required to keep the Rtg3 protein in an inactive state, which is (one of) the essential function(s) of the 14-3-3 proteins.
authors.affiliation: Section of Yeast Genetics, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. Heusden@RULBIM.Leidenuniv.nl
authors.forename: G P
authors.initials: GP
authors.lastname: van Heusden
authors.forename: H Y
authors.initials: HY
authors.lastname: Steensma
mesh_terms: D048948:14-3-3 Proteins; D000935:Antifungal Agents / Q000494:pharmacology; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D020123:Sirolimus / Q000494:pharmacology; D014157:Transcription Factors; D014446:Tyrosine 3-Monooxygenase / Q000502:physiology*
chemical_list: D048948:14-3-3 Proteins; D000935:Antifungal Agents; C075852:BMH1 protein, S cerevisiae; C097164:BMH2 protein, S cerevisiae; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C104438:RTG3 protein, S cerevisiae; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D014446:Tyrosine 3-Monooxygenase; D020123:Sirolimus