Osmotic stress up-regulates aquaporin-3 gene expression in cultured human keratinocytes.
Of ten members of the aquaporin family (AQP), the mRNA expression and regulation of AQP1, AQP3, AQP4 and AQP9 in cultured human keratinocytes were examined by an RNase protection assay. AQP3 mRNA was expressed in growing and differentiating cells, while AQP9 mRNA was only detected in differentiating cells. The epidermis in skin-equivalent cultures expressed both AQP3 and AQP9 mRNA. However, neither AQP1 nor AQP4 mRNA was detectable in either monolayer or skin-equivalent cultures. Incubation of keratinocytes in sorbitol-added hypertonic medium increased AQP3 mRNA expression. This was confirmed using other solutes such as NaCl, mannitol, glucose and sucrose. The effect of sorbitol was reversible, dose-dependent and maximal at 24 h after addition. However, AQP1, AQP4 and AQP9 mRNA expression were unchanged under any of the hypertonic conditions examined. These findings indicated that osmotic stress up-regulates AQP3 gene expression in cultured keratinocytes.
Sugiyama
Y
Y
Basic Research Laboratory, Kanebo Ltd., 5-3-28 Kotobuki-cho, 250-0002, Odawara, Japan.
Ota
Y
Y
Hara
M
M
Inoue
S
S
D051398:Aquaporin 1; D051400:Aquaporin 3; D051401:Aquaporin 4; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001789:Blood Group Antigens; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D015603:Keratinocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000032:analysis; D012965:Sodium Chloride; D013012:Sorbitol; D015854:Up-Regulation
C496020:AQP1 protein, human; C496027:AQP3 protein, human; C496030:AQP4 protein, human; C109669:AQP9 protein, human; D051401:Aquaporin 4; D020346:Aquaporins; D001789:Blood Group Antigens; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D012333:RNA, Messenger; D051398:Aquaporin 1; D051400:Aquaporin 3; D012965:Sodium Chloride; D013012:Sorbitol