Increased sputum IL-8 and IL-5 in asymptomatic nonspecific airway hyperresponsiveness.
Since asymptomatic, nonspecific airway hyperresponsiveness (BHR) may be due to an enhanced local inflammatory response, we studied molecular markers of inflammation in induced sputum from subjects with asymptomatic BHR (n = 14) compared with control subjects (n = 13) and patients with chronic obstructive pulmonary disease (COPD) (n = 10). Pulmonary lung function parameters were measured by spirometry and body plethysmography. Hyperresponsiveness was defined based on histamine challenge. Induced sputum samples were collected and the solid phase was isolated and analyzed for leukocyte numbers and differential and for cytokines (ELISA). IL-8 was 2.4-fold increased (p = 0.036) in the sputum of subjects with asymptomatic BHR (24.8 +/- 22.0 ng/mL; +/- SD) and 11.2-fold enhanced in patients with COPD (117.8 +/- 106.3 ng/mL) as compared with control subjects (10.5 +/- 7.7 ng/mL). In control subjects, no IL-5 was measured, however, sputum of those with asymptomatic BHR contained IL-5 at 0.044 +/- 0.090 ng/mL fluid and COPD patients at 1.00 +/- 2.01 ng/mL. GM-CSF could not be detected in sputum samples of any subjects investigated. Number of total leukocytes was higher in those with asymptomatic BHR and COPD (with BHR: 9.4 +/- 10.8 x 10(5); COPD: 83.5 +/- 182.5 x 10(5)) compared with persons without BHR (2.9 +/- 3.4 x 10(5)). PMN were increased in patients with asymptomatic BHR (4.1 +/- 5.3 x 10(5)) (38.8 +/- 24.7%) and COPD (32.9 +/- 71.0 x 10(5)) (75.4 +/- 18.6%) compared with controls (0.7 +/- 0.9 x 10(5)) (25.8 +/- 25.7%). In contrast to PMN counts in those with asymptomatic BHR (0.06 +/- 0.11 x 10(5)) (1.5 +/- 3.7%), eosinophil counts were only slightly increased compared with control subjects (0.01 +/- 0.02 x 10(5)) (0.6 +/- 0.9%). This study supports the hypothesis that BHR in asymptomatic people is associated with airway inflammation that may predispose to development of chronic diseases such as COPD.
Betz
R
R
Clinical Research Group Aerosols in Medicine of the GSF Institute for Inhalation Biology and the Asklepios Fachkliniken München-Gauting, Munich-Gauting, Germany.
Kohlhäufl
M
M
Kassner
G
G
Müllinger
B
B
Maier
K
K
Brand
P
P
Weber
N
N
Häussinger
K
K
Heyder
J
J
Frankenberger
M
M
D000328:Adult; D016535:Bronchial Hyperreactivity / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D001985:Bronchial Provocation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006632:Histamine; D006801:Humans; D015848:Interleukin-5 / Q000032:analysis*; D016209:Interleukin-8 / Q000032:analysis*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism / Q000503:physiopathology; D015656:Respiratory Mechanics; D012907:Smoking / Q000503:physiopathology; D013183:Sputum / Q000737:chemistry* / Q000166:cytology
D015848:Interleukin-5; D016209:Interleukin-8; D006632:Histamine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor