title: Advanced integrated mouse YAC map including BAC framework.
abstract: Functional characterization of the mouse genome requires the availability of a comprehensive physical map to obtain molecular access to chromosomal regions of interest. Positional cloning remains a crucial way of linking phenotype with particular genes. A key step and frequent stumbling block in positional cloning is making a contig of a genetically defined candidate region. The most efficient first step is isolating YAC (Yeast Artificial Chromosome) clones. A robust, detailed YAC contig map is thus an important tool. Employing Interspersed Repetitive Sequence (IRS)-PCR genomics, we have generated an advanced second-generation YAC contig map of the mouse genome that doubles both the depth of clones and the density of markers available. In addition to the primarily YAC-based map, we located 1942 BAC (Bacterial Artificial Chromosome) clones. This allows us to present for the first time a dense framework of BACs spanning the genome of the mouse, which, for instance, can serve as a nucleus for genomic sequencing. Four large-insert mouse YAC libraries from three different strains are included in our data, and our analysis incorporates the data of Hunter et al. and Nusbaum et al. There is a total of 20,205 markers on the final map, 12,033 from our own data, and a total of 56,093 YACs, of which 44,401 are positive for more than one marker.
authors.affiliation: Max-Planck-Institute of Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. L.Schalkwyk@iop.kcl.ac.uk
authors.forename: L C
authors.initials: LC
authors.lastname: Schalkwyk
authors.forename: B
authors.initials: B
authors.lastname: Cusack
authors.forename: I
authors.initials: I
authors.lastname: Dunkel
authors.forename: M
authors.initials: M
authors.lastname: Hopp
authors.forename: M
authors.initials: M
authors.lastname: Kramer
authors.forename: S
authors.initials: S
authors.lastname: Palczewski
authors.forename: J
authors.initials: J
authors.lastname: Piefke
authors.forename: S
authors.initials: S
authors.lastname: Scheel
authors.forename: M
authors.initials: M
authors.lastname: Weiher
authors.forename: G
authors.initials: G
authors.lastname: Wenske
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: H
authors.initials: H
authors.lastname: Himmelbauer
mesh_terms: D000465:Algorithms; D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D003198:Computer Simulation; D020451:Contig Mapping / Q000379:methods; D030541:Databases, Genetic; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics / Q000379:methods; D020161:Physical Chromosome Mapping / Q000379:methods*