Co-expression of VRL-1 and calbindin D-28k in the rat sensory ganglia.
The co-expression of vanilloid receptor 1-like receptor (VRL-1), a newly cloned capsaicin-receptor homologue, with calbindin D-28k was examined in the rat sensory ganglia. The co-expression was rare in the dorsal root, trigeminal and jugular ganglia and abundant in the petrosal and nodose ganglia. In the dorsal root ganglion, none of VRL-1-immunoreactive (ir) neuron co-expressed calbindin D-28k-immunoreactivity (ir). Of the VRL-1-ir neurons, 9 and 5% showed calbindin D-28k ir in the trigeminal and jugular ganglia, respectively. On the other hand, 35 and 63% of VRL-1-ir neurons in the petrosal and nodose ganglia, respectively, co-expressed these substances. The retrograde tracing method indicated that petrosal neurons which co-expressed VRL-1-and calbindin D-28k-ir innervated taste buds in the circumvallate papilla. The present findings may suggest that VRL-1 is associated with chemosensory functions in visceral sensory neurons.
Ichikawa
H
H
Department of Oral Function and Anatomy, Biodental Research Center, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. hiroichi@md.okayama-u.ac.jp
Sugimoto
T
T
D000818:Animals; D064026:Calbindins; D002628:Chemoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D017950:Ganglia, Sensory / Q000166:cytology / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism; D005930:Glossopharyngeal Nerve / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009620:Nodose Ganglion / Q000166:cytology / Q000378:metabolism; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology; D050916:TRPV Cation Channels; D013649:Taste / Q000502:physiology; D013650:Taste Buds / Q000166:cytology / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology / Q000378:metabolism; D017833:Visceral Afferents / Q000166:cytology / Q000378:metabolism*
D064026:Calbindins; D011955:Receptors, Drug; D064030:S100 Calcium Binding Protein G; D050916:TRPV Cation Channels; C494484:TRPV1 receptor