Mice lacking Ca(v)2.3 (alpha1E) calcium channel exhibit hyperglycemia.
To investigate the functional role of Ca(v)2.3 channel in glucose homeostasis, we performed in vivo glucose tolerance and insulin tolerance tests together with stress-induced glucose release tests using mice deficient in Ca(v)2.3 channel (Ca(v)2.3-/-). The Ca(v)2.3-/- mice were significantly heavier than wild-type mice. In glucose tolerance and insulin tolerance tests, Ca(v)2.3-/- mice showed a significantly higher blood glucose level compared to wild-type mice. However, stress-induced blood glucose changes in Ca(v)2.3-/- mice were similar to those in wild-type mice. These results suggest that Ca(v)2.3 channel plays a role in glucose homeostasis by reducing insulin sensitivity and that Ca(v)2.3-/- mice exhibit symptoms resembling non-insulin-dependent diabetes mellitus.
Matsuda
Y
Y
Department of Pharmacology and Neurobiology, Tokyo Medical and Dental University, Tokyo, Japan.
Saegusa
H
H
Zong
S
S
Noda
T
T
Tanabe
T
T
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015220:Calcium Channels / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D003080:Cold Temperature / Q000009:adverse effects; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005951:Glucose Tolerance Test; D006706:Homeostasis; D006943:Hyperglycemia / Q000209:etiology* / Q000378:metabolism*; D007103:Immobilization / Q000009:adverse effects; D007328:Insulin / Q000008:administration & dosage / Q000097:blood; D051379:Mice; D018345:Mice, Knockout; D013312:Stress, Physiological / Q000097:blood
D001786:Blood Glucose; C498916:Cacna1e protein, mouse; D015220:Calcium Channels; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D007328:Insulin