Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR.
A cDNA encoding the porcine type 1 insulin-like growth factor receptor (IGF1R) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The sequence of a 4.2-kb product was determined and had an open reading frame, encoding 1367 amino acids with 98.1 and 95.2% sequence similarity to the human and rat IGF1R, respectively. In the comparison of RT-PCR derived IGF1R sequences from 12 unrelated pigs, 12 silent sequence variants were found.
Harumi
T
T
Animal Genetic Engineering Laboratory, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. haru90@affrc.go.jp
Maruyama
K
K
Kagami
H
H
Sano
A
A
Matsubara
Y
Y
Tagami
T
T
Naito
M
M
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D011110:Polymorphism, Genetic; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000235:genetics*
D018076:DNA, Complementary; D017526:Receptor, IGF Type 1