DNA/polyethylenimine transfection particles: influence of ligands, polymer size, and PEGylation on internalization and gene expression.
Receptor-binding ligands have been incorporated into DNA/polyethylenimine (PEI) complexes to enhance cell binding and cellular internalization. This study characterizes receptor-mediated uptake of DNA/PEI complexes on a cellular basis. A novel assay based on flow cytometry was applied, discriminating between total cell-associated and extracellularly bound DNA complexes. Receptor-mediated uptake of ligand-containing DNA/PEI (molecular weight, 800 kd) complexes was found to occur quickly (within 1 hour), whereas unspecific uptake through adsorptive endocytosis is less efficient or requires extended periods to reach the same degree of internalization. Rapid, receptor-mediated internalization requires a small complex size; however, large, aggregated complexes show higher gene expression. Using PEI 25 kd conjugated to large proteins such as transferrin or antibodies, improper condensation with DNA leads to suboptimal uptake and gene expression, whereas partial replacement of ligand-PEI with unconjugated PEI increases both uptake and transfection. In contrast, the 8 kd protein epidermal growth factor conjugated to PEI 25 kd properly condenses DNA and mediates specific uptake into human adenocarcinoma (KB) cells. Modification of the complex surface with appropriate amounts of poly(ethylene glycol) (PEG) does not block ligand-mediated internalization. A higher degree of PEGylation reduces the internalization of transferrin or antibody-containing complexes to a level similar to that of ligand-free complexes. In contrast, epidermal growth factor "mediated uptake is less effected by excessive PEGylation.
Ogris
M
M
Institute of Biochemistry, University of Vienna, Vienna, Austria. manfred.ogris@cup.uni-muenchen.de
Steinlein
P
P
Carotta
S
S
Brunner
S
S
Wagner
E
E
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers; D004705:Endocytosis; D004815:Epidermal Growth Factor / Q000737:chemistry; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D019169:Jurkat Cells; D007624:KB Cells; D008024:Ligands; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D016853:Muromonab-CD3 / Q000737:chemistry; D010957:Plasmids; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry; D013499:Surface Properties; D014162:Transfection; D014168:Transferrin / Q000737:chemistry
D004337:Drug Carriers; D008024:Ligands; D016853:Muromonab-CD3; D014168:Transferrin; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D011094:Polyethyleneimine; D004247:DNA; D008156:Luciferases