Characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein.
A human cytomegalovirus mutant (TNsubIE2P) was constructed with alanine substitutions of four residues (T27, S144, T233, and S234) previously shown to be phosphorylated in the immediate-early 2 (IE2) protein. This mutant grew as well as the wild type at both low and high multiplicities of infection. The mutant activated the major immediate-early, UL4, and UL44 promoters to similar levels, and with similar kinetics, as wild-type virus. However, the TNsubIE2P mutant virus transactivated an endogenous simian virus 40 early promoter 4 h earlier and to higher levels than the wild-type virus in infected human fibroblasts. The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined.
Heider
Julie A
JA
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA.
Yu
Yongjun
Y
Shenk
Thomas
T
Alwine
James C
JC
D019943:Amino Acid Substitution / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008562:Membrane Glycoproteins; D009154:Mutation / Q000235:genetics*; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D025842:SUMO-1 Protein / Q000235:genetics / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D015534:Trans-Activators; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D004268:DNA-Binding Proteins; C072217:ICP36 protein, Cytomegalovirus; C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D012367:RNA, Viral; D025842:SUMO-1 Protein; D015534:Trans-Activators; C077406:UL115 protein, Human herpesvirus 5; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; C439068:glycoprotein O, cytomegalovirus