Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus.
The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
Monk
S A
SA
Department of Pharmacology, The Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
Desai
K
K
Brady
C A
CA
Williams
J M
JM
Lin
L
L
Princivalle
A
A
Hope
A G
AG
Barnes
N M
NM
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D006624:Hippocampus / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000276:immunology* / Q000378:metabolism*; D044406:Receptors, Serotonin, 5-HT3
D000906:Antibodies; D007106:Immune Sera; D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3