Effects of calcitriol and its analogues on interaction of MCP-1 and monocyte derived dendritic cells in vitro.
OBJECTIVE
To study the effect of calcitriol [1,25(OH)2D3] and its analogues on the interaction of monocyte chemoattractant protein-1 (MCP-1) and in vitro generated monocyte-derived dendritic cells (MoDC).


METHODS
MoDC were obtained by differentiating monocytes in exposure to GM-CSF and IL-4 for 5 d. mRNA expression of MCP-1 and its receptors were analyzed by RT-PCR, and protein production of MCP-1 by ELISA and migratory ability of MoDC in response to MCP-1 by a micromultiwell chemotaxis chamber assay.


RESULTS
MoDC can express MCP-1 mRNA, and secret a low level of MCP-1 protein and has the ability to migrate to MCP-1 in corresponding to its expression of MCP-1 receptors. Calcitriol and its analogues with the same affinity to vitamin D receptor up-regulated the gene expression of both MCP-1 and its receptors, enhanced MCP-1 protein production and promoted the migratory ability of MoDC to MCP-1.


CONCLUSIONS
The interaction of DC and MCP-1 found in this study may suggest a possible auto-regulatory role between DC and MCP-1 and the modulatory effect of calcitriol and its analogues on DC and MCP-1 might provide an understanding of their positive role in tumors.
Zhu
K J
KJ
Department of Dermatology, 2nd Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou 310009, China. zhukejian@mail.hz.zj.cn
Shen
Q Y
QY
Zheng
M
M
Mrowietz
U
U
D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000494:pharmacology; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D054390:Receptors, CCR2; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics
C515334:CCR2 protein, human; D018932:Chemokine CCL2; D004100:Dihydroxycholecalciferols; D012333:RNA, Messenger; D054390:Receptors, CCR2; D019707:Receptors, Chemokine; C055085:calcipotriene; C023850:1 alpha,24-dihydroxyvitamin D3; D002117:Calcitriol