Subcellular distribution of aquaporin 5 in salivary glands in primary Sjögren's syndrome.
Secretions from salivary and lacrimal glands are reduced in patients with primary Sjögren's syndrome (PSS). Since aquaporin 5 is involved in transport of water and is present in salivary and lacrimal glands, this protein was thought to have a major role in the pathogenesis of PSS. We used indirect immunofluorescence and an immunoperoxidase technique to assess expression and subcellular localisation of aquaporin 5 in patients and controls. Our results suggest that the distribution and density of aquaporin 5 in salivary glands does not differ between patients with PSS and those without. Thus, the role of aquaporin 5 in the pathogenesis of PSS needs to be reassessed and alternative pathogenetic mechanisms investigated.
Beroukas
D
D
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre, SA 5042, Bedford Park, Australia.
Hiscock
J
J
Jonsson
R
R
Waterman
S A
SA
Gordon
T P
TP
D000818:Animals; D051402:Aquaporin 5; D020346:Aquaporins / Q000378:metabolism*; D016022:Case-Control Studies; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D007765:Lacrimal Apparatus / Q000378:metabolism*; D008565:Membrane Proteins; D051381:Rats; D012469:Salivary Glands / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000209:etiology / Q000378:metabolism*
C496036:AQP5 protein, human; C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D020346:Aquaporins; D008565:Membrane Proteins