A novel multifunctional labeling reagent for enhanced protein characterization with mass spectrometry.
Individual peptides with lysine at the C-terminus as well as protein tryptic digests were reacted with 2-methoxy-4,5-dihydro-1H-imidazole, converting lysine residues to their 4,5-dihydro-1H-imidazol-2-yl derivatives. The mass spectra of derivatized digests exhibit a greater number of more intense features than their underivatized counterparts, thus increasing the information obtained in peptide mapping experiments. Additionally, MS/MS spectra of the derivatized peptides are greatly simplified in comparison to their native species, yielding primarily an easily interpretable series of y-ions. Finally, this novel label also enables differential quantitation studies, as a stable isotopic form containing four deuterium atoms can readily be produced.
Peters
E C
EC
The Genomics Institute of the Novartis Research Foundation, 3115 Merryfield Row, San Diego, CA 92121, USA. peters@gnf.org
Horn
D M
DM
Tully
D C
DC
Brock
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002138:Calibration; D006736:Horses; D006868:Hydrolysis; D007093:Imidazoles / Q000737:chemistry; D007202:Indicators and Reagents; D008239:Lysine / Q000032:analysis; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000737:chemistry*; D020543:Proteome / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D014357:Trypsin
C447410:2-methoxy-4,5-dihydro-1H-imidazole; D007093:Imidazoles; D007202:Indicators and Reagents; D009211:Myoglobin; D010446:Peptide Fragments; D011506:Proteins; D020543:Proteome; D014357:Trypsin; D008239:Lysine