title: Constraints, histones, and the 30-nm spiral.
abstract: We investigate the mechanical stability of a segment of DNA wrapped around a histone in the nucleosome configuration, under the assumption that the proper model for this packaging arrangement is that of an elastic rod that is free to twist and that writhes subject to mechanical constraints. We find that the number of constraints required to stabilize the nuclesome configuration is determined by the length of the segment, the number of times the DNA wraps around the histone spool, and the specific constraints utilized. While it can be shown that four constraints suffice, in principle, to insure stability of the nucleosome, a proper choice must be made to guarantee the effectiveness of this minimal number. The optimal choice of constraints appears to bear a relation to the existence of a spiral ridge on the surface of the histone octamer. The particular configuration that we investigate is related to the 30-nm spiral, a higher-order organization of DNA in chromatin.
authors.affiliation: Department of Physics, UCLA, Box 951547, Los Angeles, California 90095-1547, USA.
authors.forename: R
authors.initials: R
authors.lastname: Zandi
authors.forename: J
authors.initials: J
authors.lastname: Rudnick
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D004247:DNA / Q000737:chemistry; D004355:Drug Stability; D006657:Histones / Q000737:chemistry*; D008958:Models, Molecular; D009707:Nucleosomes / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D006657:Histones; D009707:Nucleosomes; D004247:DNA