Effect of p53 on centrosome amplification in prostate cancer cells.
Chromosomal instability (CIN) is one of the common features in prostate cancer, especially in advanced stages. Recently, the involvement of p53 in CIN through the regulation of centrosome amplification has been proposed in certain tumor types. In this study, we investigated the relationship between p53 and centrosome amplification in prostate cancer cells. Increased centrosome number and size were observed in DU145 and PC3 containing nonfunctional p53 compared to LNCap which expressed wild-type p53. Transfection of p53 into PC3 cells resulted in a decreased cell growth rate, G2/M arrest and decreased centrosome abnormalities. We provide the first evidence on a correlation between loss of p53 function and centrosome amplification in prostate cancer cells. Our results indicate that p53 may play a role in the regulation of centrosome amplification and loss of p53 may be one of the mechanisms involving CIN in prostate cancer cells.
Ouyang
X
X
Department od Anatomy, Faculty of Medicine, University of Hong Kong, SAR, PR China.
Wang
X
X
Xu
K
K
Jin
D Y
DY
Cheung
A L
AL
Tsao
S W
SW
Wong
Y C
YC
D018385:Centrosome / Q000378:metabolism* / Q000473:pathology; D002873:Chromosome Fragility / Q000235:genetics; D016195:G2 Phase; D006801:Humans; D008297:Male; D008938:Mitosis; D011471:Prostatic Neoplasms; D013194:Staining and Labeling; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D016159:Tumor Suppressor Protein p53