Increased density of galanin binding sites in the dorsal raphe in a genetic rat model of depression.
The Flinders Sensitive Line rats showed an increased immobility response by 104% (P<0.01) in the forced swim test with respect to the Flinders Resistant Line rats (control). The Flinders Sensitive Line rats also had an increase of [(125)I]galanin (porcine) binding in the dorsal raphe (55+/-3.3 fmol/mg protein) with respect to the Flinders Resistant Line rats (38+/-3.6 fmol/mg protein) (42%, P<0.05) without changes in galanin receptor affinity and with a significant reduction (32.3%, P<0.05) of galanin-like immunoreactivity in the dorsal raphe. The results indicate that enhancement of galanin receptor function in the dorsal raphe rich in 5-HT neurons could be a mechanism involved in the production of depressive-like activity in this animal model of depression.
Bellido
Inmaculada
I
Department of Pharmacology and Clinical Therapeutics, School of Medicine, University of Malaga, Malaga, Spain. ibellido@uma.es
Díaz-Cabiale
Zaida
Z
Jiménez-Vasquez
Patricia A
PA
Andbjer
Beth
B
Mathé
Aleksander A
AA
Fuxe
Kjell
K
D000818:Animals; D001667:Binding, Competitive; D003863:Depression / Q000235:genetics / Q000503:physiopathology*; D004195:Disease Models, Animal; D019004:Galanin / Q000502:physiology*; D006380:Helplessness, Learned; D008297:Male; D009474:Neurons / Q000378:metabolism; D011903:Raphe Nuclei / Q000378:metabolism*; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000378:metabolism*; D012701:Serotonin / Q000502:physiology; D013550:Swimming; D013552:Swine; D015854:Up-Regulation
D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide; D012701:Serotonin; D019004:Galanin