Lecithin: cholesterol acyltransferase is insufficient to prevent oxidative modification of low-density lipoprotein.
We tried to confirm the antioxidative capability of lecithin:cholesterol acyltransferase (LCAT) reported by Vohl et al. [Biochemistry (1999) 38, 5976-5981]. The enzyme solution protected LDL against oxidation. However, this protection was not due to LCAT enzyme, but to some unknown low-molecular-weight substance(s) in the solution; LCAT itself exerted little protective effect against LDL oxidation.
Murakami
Youhei
Y
Laboratory of Nutrition, Graduate School of Agricultural Science, and Gene Research Center, Tohoku University, Tsutsumidori, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan.
Kamiyama
Shin
S
Howlader
Zakir H
ZH
Yamato
Tokuhisa
T
Komai
Michio
M
Furukawa
Yuji
Y
D000975:Antioxidants / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007531:Isoflurophate / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011489:Protein Denaturation
D000975:Antioxidants; D004791:Enzyme Inhibitors; D008077:Lipoproteins, LDL; D007531:Isoflurophate; D004228:Dithionitrobenzoic Acid; D007862:Phosphatidylcholine-Sterol O-Acyltransferase