In vitro adaptation of a ligase ribozyme for activity under a low-pH condition.
A ligase ribozyme accelerating a ligation reaction with oligonucleotide under a low-pH condition was selected by in vitro adaptation. A ribozyme active at pH 7 was randomly mutated, and the resultant RNA library was subjected to in vitro adaptation under a low-pH reaction condition. At pH 4, the adapted RNAs reacted with the oligonucleotide substrates about 200 times faster than the original ribozyme. When the ribozyme was cloned and sequenced, 10 of the 30 clones sequenced had identical sequences. The differences in sequence from the original ribozyme were found at four positions in the middle region and at the 3' end. A few sequential differences dominated the activity of the ribozyme under the extreme condition. The adapted ribozyme had one repeating sequence that was critical for the activity.
Miyamoto
Y
Y
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Japan.
Teramoto
N
N
Imanishi
Y
Y
Ito
Y
Y
D001483:Base Sequence; D003001:Cloning, Molecular; D006863:Hydrogen-Ion Concentration; D008025:Ligases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000502:physiology; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D009841:Oligonucleotides; D016337:RNA, Catalytic; D012313:RNA; D008025:Ligases