title: Apoptosis in neural crest cells by functional loss of APC tumor suppressor gene.
abstract: Apc is a gene associated with familial adenomatous polyposis coli (FAP) and its inactivation is a critical step in colorectal tumor formation. The protein product, adenomatous polyposis coli (APC), acts to down-regulate intracellular levels of beta-catenin, a key signal transducer in the Wnt signaling. Conditional targeting of Apc in the neural crest of mice caused massive apoptosis of cephalic and cardiac neural crest cells at about 11.5 days post coitum, resulting in craniofacial and cardiac anomalies at birth. Notably, the apoptotic cells localized in the regions where beta-catenin had accumulated. In contrast to its role in colorectal epithelial cells, inactivation of APC leads to dysregulation of beta-catenin/Wnt signaling with resultant apoptosis in certain tissues including neural crest cells.
authors.affiliation: Department of Cell Biology, The Cancer Institute, Japanese Foundation for Cancer Research, 1-37-1 Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan.
authors.forename: Sumitaka
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akazawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okada
authors.forename: Akiteru
authors.initials: A
authors.lastname: Maeno
authors.forename: Masaki
authors.initials: M
authors.lastname: Ito
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Sugitani
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shibata
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Miyazaki Ji
authors.forename: Motoya
authors.initials: M
authors.lastname: Katsuki
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Yamauchi
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yamamura Ki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Katamine
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Noda
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics* / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D024043:Collagen Type II / Q000378:metabolism; D003106:Colon / Q000196:embryology; D003433:Crosses, Genetic; D003598:Cytoskeletal Proteins / Q000378:metabolism; D015536:Down-Regulation; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005697:Galactosides / Q000378:metabolism; D016158:Genes, p53 / Q000235:genetics; D005838:Genotype; D006321:Heart / Q000196:embryology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D007211:Indoles / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009432:Neural Crest / Q000166:cytology* / Q000473:pathology; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012007:Rectum / Q000196:embryology; D015398:Signal Transduction; D013997:Time Factors; D015534:Trans-Activators; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C495267:CTNNB1 protein, mouse; D024043:Collagen Type II; D003598:Cytoskeletal Proteins; D005697:Galactosides; D007211:Indoles; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside