Total and structured water in cancer: an NMR experimental study of serum and tissues in DMBA-induced OF1 mice.
Total water and structured water (fraction of total water which remains unfrozen below the transition point from the semisolid to solid state) were characterized by 1H NMR relaxometry in the sera and tissues of 3 groups of 30 female mice (C, H and L) receiving a single administration of DMBA and different diets. Mice given the diet H, containing the highest proportion of saturated fatty acids and processed starch, and the lowest phytochemicals content, presented the highest tumor incidence (lymphoma). This allowed 3 subgroups to be defined: subnormal (SN), small (T+) and large tumor (T++). Spin-lattice relaxation times of total water (Tlobs) in the sera and tissues did not significantly differ between C, H and L groups, and SN, T+ and T++ subgroups. In T+ mice, a decrease in the relative amount of structured water was noticed in the serum, liver and heart, while changes in the temperature dependence of the Tl of structured water (Tlsw) were observed between -21 degrees C and -42 degrees C. These results suggest a moderate increase in the rotational mobility of structured water molecules in the serum and the heart, and a pronounced decrease in the liver. Likewise, the modification of the Tlsvv temperature dependence curve's shape tends to confirm the existence of important conformational changes in the macromolecular assemblies, which markedly affect the properties of structured water, especially in the earliest stage of cancer development.
Pouliquen
D
D
EMI 00/18 INSERM, Laboratoire de Biochimie et Biologie Moléculaire, Angers, France. daniel.pouliquen@univ-angers.fr
Foussard
F
F
Tanguy
G
G
Roux
J
J
Malthièry
Y
Y
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002273:Carcinogens; D004032:Diet; D005260:Female; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000097:blood / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D013696:Temperature; D014867:Water / Q000737:chemistry / Q000378:metabolism*
D001798:Blood Proteins; D002273:Carcinogens; D014867:Water; D015127:9,10-Dimethyl-1,2-benzanthracene