title: O-carboxymethyl-chitin concentration in granulocytes during bone repair.
abstract: Peroral and intravenous administrations of (14)C-labeled carboxymethyl-chitin (CM-chitin) revealed that CM-chitin accumulated in bone marrow. Thus, a composite of CM-chitin with hydroxyapatite (HA) was prepared to examine the bone repairing properties by animal and cell line experiments. The new bone formation of CM-chitin.HA composite was superior to that of CM-chitin, HA, and blank. A porous CM-chitin.HA composite is a functional material which could act as a scaffolding of osteoblast-like cells, a barrier to ingrowth of fibrous connective tissues. The cytotoxicity of CM-chitin was evaluated using the MC3T3-E1 cell line, and we found that control of degree of deacetylation is a very important factor in using CM-chitin as bone repairing material.
authors.affiliation: Faculty of Engineering and HRC, Kansai University, Suita, Osaka 564-8680, Japan. stokura@ipcku.kansai-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Tokura
authors.forename: H
authors.initials: H
authors.lastname: Tamura
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics* / Q000627:therapeutic use* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics* / Q000627:therapeutic use* / Q000633:toxicity; D005269:Femur / Q000473:pathology; D006098:Granulocytes / Q000378:metabolism*; D007275:Injections, Intravenous; D008252:Macaca fascicularis; D051379:Mice; D016348:Osseointegration; D011817:Rabbits; D014018:Tissue Distribution
chemical_list: D001672:Biocompatible Materials; D002686:Chitin; C044872:O-(carboxymethyl)chitin