Conjugation between bacterial and mammalian cells.
Bacterial conjugation, in which DNA is transferred from one bacterium to another, was first reported in 1946 and found to be mediated by the F factor. Although the F and RK2/RP4 prototypic plasmids can mediate the transfer of DNA from bacteria to yeast, there has been no evidence of classical bacterial conjugation to higher eukaryotes. Here, I present evidence of such transfer, using Escherichia coli, the RK2 plasmid system and Chinese hamster ovary CHO K1 cells.
Waters
V L
VL
Department of Medicine, School of Medicine University of California, San Diego, La Jolla, California 92093-0640, USA. vwaters@ucsd.edu
D000818:Animals; D016466:CHO Cells; D003227:Conjugation, Genetic; D006224:Cricetinae; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids
D004269:DNA, Bacterial