Regulation of expression of two novel flower-specific genes from tomato (Solanum lycopersicum) by gibberellin.
Two flower-specific cDNAs have been isolated after differential screening of an anther cDNA library. This library was constructed 48 h after GA(3) treatment of buds of the GA-deficient gib-1 mutant of tomato. Northern blot analysis during flower development in tomato demonstrated that the expression of both genes is regulated by gibberellins (GAs). Application of GA(3) to developmentally arrested gib-1 flower buds induced new expression of tgas100 mRNA 48 h post-treatment, while an increased accumulation of tgas105 mRNA was found after 8 h. In situ analyses showed the spatial distribution of the expression of both genes within the tomato flower. One of the deduced polypeptides (TGAS105) displays similarities to cysteine-rich extensin-like proteins, while the other (TGAS100) shows significant homology with a stamen-specific gene of Antirrhinum majus. Based on the deduced protein sequences, the possible function of the encoded proteins is discussed.
Van den Heuvel
Koen J
KJ
Department of Experimental Botany, Research Group Molecular Plant Physiology, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
Van Lipzig
Rosalinde H
RH
Barendse
Gerard W
GW
Wullems
George J
GJ
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology*; D017403:In Situ Hybridization; D018551:Solanum lycopersicum / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; C007842:gibberellic acid