The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system.
The effect of dissolved oxygen concentration on human secreted alkaline phosphatase (SEAP) glycosylation by the insect cell-baculovirus expression system was investigated in a well-controlled bioreactor. Oligomannose-type N-linked glycans (i.e., Man2 to Man6 and Man3F) were present in SEAP produced by Spodoptera frusiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines. The relative amounts of the most highly processed glycans (i.e., Man3F and Man2 in the SEAP from Sf-9 and Tn-5B1-4 cells, respectively) were significantly higher at 50% of air saturation than at either 10% or 190% of air saturation. That is, glycan processing was inhibited at both low and high dissolved oxygen concentrations.
Zhang
Fuming
F
Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, USA.
Saarinen
Mark A
MA
Itle
Laura J
LJ
Lang
Sybil C
SC
Murhammer
David W
DW
Linhardt
Robert J
RJ
D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D019149:Bioreactors; D002460:Cell Line; D005822:Genetic Vectors; D006031:Glycosylation; D006801:Humans; D010100:Oxygen / Q000737:chemistry*; D011134:Polysaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D018411:Spodoptera
D011134:Polysaccharides; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D010100:Oxygen