title: Phenotypic adaptations in human muscle fibers 6 and 24 wk after spinal cord injury.
abstract: The effects of spinal cord injury (SCI) on the profile of sarco(endo) plasmic reticulum calcium-ATPase (SERCA) and myosin heavy chain (MHC) isoforms in individual vastus lateralis (VL) muscle fibers were determined. Biopsies from the VL were obtained from SCI subjects 6 and 24 wk postinjury (n = 6). Biopsies from nondisabled (ND) subjects were obtained at two time points 18 wk apart (n = 4). In ND subjects, the proportions of VL fibers containing MHC I, MHC IIa, and MHC IIx were 46 +/- 3, 53 +/- 3, and 1 +/- 1%, respectively. Most MHC I fibers contained SERCA2. Most MHC IIa fibers contained SERCA1. All MHC IIx fibers contained SERCA1 exclusively. SCI resulted in significant increases in fibers with MHC IIx (14 +/- 4% at 6 wk and 16 +/- 2% at 24 wk). In addition, SCI resulted in high proportions of MHC I and MHC IIa fibers with both SERCA isoforms (29% at 6 wk and 54% at 24 wk for MHC I fibers and 16% at 6 wk and 38% at 24 wk for MHC IIa fibers). Thus high proportions of VL fibers were mismatched for SERCA and MHC isoforms after SCI (19 +/- 3% at 6 wk and 36 +/- 9% at 24 wk) compared with only ~5% in ND subjects. These data suggest that, in the early time period following SCI, fast fiber isoforms of both SERCA and MHC are elevated disproportionately, resulting in fibers that are mismatched for SERCA and MHC isoforms. Thus the adaptations in SERCA and MHC isoforms appear to occur independently.
authors.affiliation: Muscle Function Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA. rjtalmadge@csupomona.edu
authors.forename: R J
authors.initials: RJ
authors.lastname: Talmadge
authors.forename: M J
authors.initials: MJ
authors.lastname: Castro
authors.forename: D F
authors.initials: DF
authors.lastname: Apple
authors.forename: G A
authors.initials: GA
authors.lastname: Dudley
mesh_terms: D000286:Administration, Sublingual; D000328:Adult; D015114:Androstenediol / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D001823:Body Composition / Q000187:drug effects; D003505:Cyclodextrins; D004032:Diet; D004958:Estradiol / Q000097:blood; D005079:Excipients; D006728:Hormones / Q000097:blood*; D006801:Humans; D008297:Male; D013739:Testosterone / Q000097:blood; D014891:Weight Lifting
chemical_list: D003505:Cyclodextrins; D005079:Excipients; D006728:Hormones; D013739:Testosterone; D004958:Estradiol; D015114:Androstenediol