title: A rapid and sensitive method for the detection of Yersinia enterocolitica strains from clinical samples.
abstract: OBJECTIVE
  To compare three culture methods to detect Yersinia enterocolitica from oral or rectal swabs from experimentally infected pigs.
  
  
  RESULTS
  The three methods used were: direct plating on Cefsulodin-Irgasan-Novobiocin (CIN) agar, cold enrichment in phosphate buffered saline (PBS) followed by plating on CIN agar and selective enrichment with Luria-Bertani-Bile Salts Irgasan (LB-BSI) followed by plating on CIN agar. Selective enrichment with LB-BSI produced the highest recovery rate (63%), when compared with cold enrichment (52%) and plating on CIN agar alone (43%). Selective enrichment with LB-BSI was significantly (P < 0.02) more sensitive than direct plating on CIN agar and more sensitive than cold enrichment (P < 0.1).
  
  
  CONCLUSIONS
  Selective enrichment with LB-BSI was more sensitive than the widely accepted method of cold enrichment and it reduced the time required for detection of Y. enterocolitica by three weeks. Selective enrichment with LB-BSI was also compatible with a multiplex PCR technique.
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, College of Science, Massey University, Palmerston North, New Zealand.
authors.forename: H M
authors.initials: HM
authors.lastname: Hussein
authors.forename: S G
authors.initials: SG
authors.lastname: Fenwick
authors.forename: J S
authors.initials: JS
authors.lastname: Lumsden
mesh_terms: D000362:Agar; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D009055:Mouth / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012007:Rectum / Q000382:microbiology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013997:Time Factors; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D000362:Agar