Early mesodermal phenotypes in splotch suggest a role for Pax3 in the formation of epithelial somites.
The paired box containing transcription factor Pax3 is a crucial regulator of dermomyotome and muscle development. However, the allelic series of Pax3/Splotch mutants also displays characteristic vertebral column malformations, which do not result from defective dorsoventral somite pattern. Rather, vertebral column and sclerotomal phenotypes are reminiscent of the phenotypes observed in the segmentation/somitogenesis mutants rachiterata and pudgy. Moreover, rostrocaudal somite pattern and somitic boundaries are disturbed in Splotch as monitored by the expression of Uncx4.1 and Lunatic fringe. Alterations in EphA4, Dll1, and Uncx4.1 expression are evident already in the condensing paraxial mesoderm, correlating with the first phase of Pax3 expression before and during somite formation. This finding suggests an early function of Pax3 during the formation of epithelial somites.
Schubert
F R
FR
King's College London, MRC Centre for Developmental Neurobiology, New Hunt's House, London, United Kingdom.
Tremblay
P
P
Mansouri
A
A
Faisst
A M
AM
Kammandel
B
B
Lumsden
A
A
Gruss
P
P
Dietrich
S
S
D000818:Animals; D004268:DNA-Binding Proteins / Q000502:physiology*; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology*; D008648:Mesoderm / Q000502:physiology*; D051379:Mice / Q000196:embryology; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains / Q000235:genetics*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D010641:Phenotype; D013131:Spine / Q000196:embryology; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C068686:Pax3 protein, mouse