Effects of proteasome inhibitors on the synaptic localization of Vesl-1S/Homer-1a proteins.
The Vesl-1S/Homer-1a proteins are upregulated during seizure and long-term potentiation, but are rapidly degraded by ubiquitin-proteasome systems under normal conditions. We examined the distribution of Vesl-1S proteins in cultured hippocampal neurons. Application of proteasome inhibitors caused accumulation of Vesl-1S immunoreactivity in the neurons which showed a punctate distribution in the cortical regions of the cells, and these puncta were found to be juxtaposed with synaptophysin, a presynaptic, synapse-specific protein. These results suggest that Vesl-1S protein is synaptically targeted.
Ageta
H
H
Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
Kato
A
A
Fukazawa
Y
Y
Inokuchi
K
K
Sugiyama
H
H
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology; D000072233:Homer Scaffolding Proteins; D007150:Immunohistochemistry; D007976:Leupeptins / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D009479:Neuropeptides / Q000032:analysis / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D013569:Synapses / Q000737:chemistry / Q000378:metabolism*; D016708:Synaptophysin / Q000032:analysis
D002352:Carrier Proteins; D015853:Cysteine Proteinase Inhibitors; D000072233:Homer Scaffolding Proteins; D007976:Leupeptins; D009097:Multienzyme Complexes; D009479:Neuropeptides; D016708:Synaptophysin; C067713:lactacystin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde; D000111:Acetylcysteine