Monoalkylether phospholipids in the sulfate-reducing bacteria Desulfosarcina variabilis and Desulforhabdus amnigenus.
In this study, cellular lipid compositions of two mesophilic sulfate-reducing bacteria were analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS). In Desulfosarcina variabilis and Desulforhabdus amnigenus, alkylether-containing phospholipids were detected which had previously only been found in significant amounts in deeply branching hyperthermophilic bacteria and archaea. Combining information from HPLC-MS analysis and chemical degradation experiments, ether lipids were identified as 1-alkyl-2-acyl-phosphatidyl ethanolamines, glycerols and cholines. In Desulforhabdus amnigenus, n-penta-, n-hexa- and n-heptadecyl ethers were present (in order of decreasing abundance), whereas Desulfosarcina variabilis solely contained n-hexadecyl ether side chains.
Rütters
H
H
Organic Geochemistry Group, Institute of Chemistry and Biology of the Marine Environment, Carl von Ossietzky University of Oldenburg, P.O. Box 2503, 26111 Oldenburg, Germany. heike.ruetters@icbm.de
Sass
H
H
Cypionka
H
H
Rullkötter
J
J
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010743:Phospholipids / Q000032:analysis*; D010955:Plasmalogens / Q000032:analysis*; D016999:Sulfur-Reducing Bacteria / Q000737:chemistry*
D010743:Phospholipids; D010955:Plasmalogens