title: Essential contribution of germline-encoded lysine residues in Jgamma1.2 segment to the recognition of nonpeptide antigens by human gammadelta T cells.
abstract: Human gammadelta T cells display unique repertoires of Ag specificities largely imposed by selective usages of distinct Vgamma and Vdelta genes. Among them, Vgamma2/Vdelta2(+) T cells predominate in the circulation of healthy adults and respond to various microbial small molecular mass nonpeptide Ags. The present results indicate that the primary Vgamma2/Vdelta2(+) T cells stimulated with the distinct groups of nonpeptide Ags, including monoethyl pyrophosphate, isobutyl amine, and aminobisphosphonate, invariably exhibit Jgamma1.2 in the Vgamma2(+) TCR-gamma chains. Gene transfer studies revealed that most of the randomly cloned Vgamma2/Jgamma1.2(+) TCR-gamma genes bearing diverse Vgamma/Jgamma junctional sequences could confer the responsiveness to all these nonpeptide Ags, while none of the Vgamma2/Jgamma1.1(+) or Vgamma2/Jgamma1.3(+) TCR-gamma genes could do so. Furthermore, mutation of the lysine residues encoded by the Jgamma1.2 gene, which are unique in human Jgamma1.2 and absent in other human or mouse Jgamma segments, completely abrogated the responsiveness to all the nonpeptide Ags without affecting the response to anti-CD3 mAb. These results strongly suggested that the positively charged lysine residues in the TCR-gamma chain CDR3 region encoded by the germline Jgamma1.2 gene play a key role in the recognition of diverse small molecular mass nonpeptide Ags.
authors.affiliation: Department of Immunology and Cell Biology, Graduate School of Medicine, and Graduate School of Biostudies, Kyoto University, Kyoto, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Miyagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: B
authors.initials: B
authors.lastname: Mikami
authors.forename: K
authors.initials: K
authors.lastname: Danno
authors.forename: M
authors.initials: M
authors.lastname: Uehara
authors.forename: N
authors.initials: N
authors.lastname: Minato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D002082:Butylamines / Q000276:immunology; D002999:Clone Cells; D022801:Complementarity Determining Regions; D004164:Diphosphonates / Q000276:immunology; D019675:Genes, T-Cell Receptor gamma; D006801:Humans; D007133:Immunoglobulin Joining Region; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D008239:Lysine / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D000077268:Pamidronate; D010455:Peptides / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000941:Antigens; D002082:Butylamines; D022801:Complementarity Determining Regions; D004164:Diphosphonates; D007133:Immunoglobulin Joining Region; D010455:Peptides; D016692:Receptors, Antigen, T-Cell, gamma-delta; C053521:isobutylamine; D008239:Lysine; D000077268:Pamidronate