title: Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera.
abstract: The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.
authors.affiliation: Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Szent-Györgyi A. 4., H-2100, Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Semsey
authors.forename: Béla
authors.initials: B
authors.lastname: Blaha
authors.forename: Krisztián
authors.initials: K
authors.lastname: Köles
authors.forename: László
authors.initials: L
authors.lastname: Orosz
authors.forename: Péter P
authors.initials: PP
authors.lastname: Papp
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012361:RNA, Transfer, Pro / Q000235:genetics*; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000821:virology*; D013045:Species Specificity; D016662:Virus Integration / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D012361:RNA, Transfer, Pro