Characterization of G3BPs: tissue specific expression, chromosomal localisation and rasGAP(120) binding studies.
The G3BP (ras-GTPase-Activating Protein SH3-Domain-Binding Protein) family of proteins has been implicated in both signal transduction and RNA-metabolism. We have previously identified human G3BP-1, G3BP-2, and mouse G3BP-2. Here, we report the cloning of mouse G3BP-1, the discovery of two alternatively spliced isoforms of mouse, and human G3BP-2 (G3BP-2a and G3BP-2b), and the chromosomal localisation of human G3BP-1 and G3BP-2, which map to 5q14.2-5q33.3 and 4q12-4q24 respectively. We mapped the rasGAP(120) interactive region of the G3BP-2 isoforms and show that both G3BP-2a and G3BP-2b use an N-terminal NTF2-like domain for rasGAP(120) binding rather than several available proline-rich (PxxP) motifs found in members of the G3BPs. Furthermore, we have characterized the protein expression of both G3BP-1 and G3BP-2a/b in adult mouse tissues, and show them to be both tissue and isoform specific.
Kennedy
D
D
The Institute for Molecular Bioscience and the Department of Biochemistry, University of Queensland, Brisbane, Queensland, Australia. Derek.Kennedy@mailbox.gu.edu.au
French
J
J
Guitard
E
E
Ru
K
K
Tocque
B
B
Mattick
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular / Q000379:methods; D004265:DNA Helicases; D006801:Humans; D007150:Immunohistochemistry; D007313:Insecta / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D016601:RNA-Binding Proteins / Q000378:metabolism; D017385:Sequence Homology; D015398:Signal Transduction / Q000502:physiology; D014018:Tissue Distribution / Q000502:physiology; D020729:p120 GTPase Activating Protein / Q000378:metabolism*
D002352:Carrier Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000067776:RNA Recognition Motif Proteins; D016601:RNA-Binding Proteins; D020729:p120 GTPase Activating Protein; D004265:DNA Helicases; C116070:G3BP1 protein, human; C492055:G3bp1 protein, mouse; D020365:RNA Helicases