title: Importance of the renal nerves for basal and stimulated renin mRNA levels in fetal and adult ovine kidneys.
abstract: OBJECTIVE
  To investigate the effect of renal denervation on renin mRNA levels in fetal and nonpregnant adult ovine renocortical tissue and in isolated juxtaglomerular cells under basal conditions and after stimulation.
  
  
  METHODS
  The left kidney was denervated and the right kidney subjected to a sham procedure in nine ovine fetuses (136-141 days' gestation) and 20 nonpregnant ewes. After 5-7 days the denervated and intact kidneys were obtained, and renin-containing renal cortical cells were isolated and cultured overnight. Then cells were treated with isoproterenol, forskolin, or isomethylbutyl xanthine (IBMX) for 4 hours. Total RNA was isolated and renin mRNA measured by RNase protection assay. Cyclic adenosine monophosphate (cAMP) levels were measured in the incubation medium with a competitive enzyme immunoassay.
  
  
  RESULTS
  In adults, basal renin mRNA levels were significantly lower in denervated than in sham-operated kidneys. No difference was noted between denervated and intact fetal kidneys. Renin mRNA levels were significantly higher in fetal than in adult kidney tissue, and cells from fetuses had greater increases in renin mRNA after stimulation than did cells from adults. Fetal cells also released more cAMP into the incubation medium, and there was a correlation between cAMP and renin mRNA levels.
  
  
  CONCLUSIONS
  The data indicate the effects of renal denervation on renin mRNA expression in the kidney are age dependent and that the fetus in late gestation has a mechanism for maintaining renin mRNA levels after denervation, which is absent or nonfunctional in the adult.
authors.affiliation: Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J W
authors.initials: JW
authors.lastname: Strandhoy
authors.forename: J C
authors.initials: JC
authors.lastname: Rose
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D003714:Denervation; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007545:Isoproterenol / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects / Q000196:embryology / Q000294:innervation* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics; D012756:Sheep
chemical_list: D000318:Adrenergic beta-Agonists; D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger; D005576:Colforsin; D000242:Cyclic AMP; D012083:Renin; D007545:Isoproterenol; D015056:1-Methyl-3-isobutylxanthine