Micronuclei and p53 accumulation in preneoplastic and malignant lesions of the head and neck.
No single biomarker can predict the risk for malignant transformation of precancerous lesions of the head and neck. Micronucleus frequency, nuclear p53 accumulation and mitotic index were determined in proliferating basal cells using paraffin-embedded specimens from normal, dysplastic and malignant tissues. p53 accumulation was detected by immunohistochemistry using pAb 1081 and pAb 240 antibodies. Micronuclei were scored in the same cell population and classified for the presence/ absence of p53 accumulation in the main nucleus. Fifty-three carcinomas and 15 precancerous lesions were studied. Both micronuclei and p53 accumulation were found in precancerous lesions, suggesting that they are early events in head and neck squamous cell carcinoma progression. The two biomarkers were not related to each other: indeed, micronucleus frequency was higher in p53-negative than in p53-positive cells. Three patients with precancerous lesions later developed carcinomas; all three cases showed high frequencies of both micronuclei and cells accumulating p53 protein.
Delfino
Valeria
V
National Cancer Research Institute, Genova, Italy.
Casartelli
Gianluigi
G
Garzoglio
Barbara
B
Scala
Marco
M
Mereu
Paola
P
Bonatti
Stefania
S
Margarino
Giovanni
G
Abbondandolo
Angelo
A
D015415:Biomarkers; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D018450:Disease Progression; D005347:Fibroblasts / Q000737:chemistry / Q000648:ultrastructure; D016193:G1 Phase; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology; D007124:Immunoenzyme Techniques; D015162:Micronucleus Tests; D008940:Mitotic Index; D009363:Neoplasm Proteins / Q000032:analysis*; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D012871:Skin Diseases / Q000235:genetics* / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D015415:Biomarkers; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53