title: Differential expression of the L-plastin gene in human colorectal cancer progression and metastasis.
abstract: To identify molecular alterations in the progression of colorectal carcinoma, we analyzed gene expression profiles of colon cancer cell lines derived from primary and metastatic tumors from a single patient. Of 2280 cDNAs investigated using our in-house microarray, the expression of 6 genes (tumor-associated antigen L6, L-plastin, the human homologue of yeast ribosomal protein S28, the B-cell translocation gene, mitochondrial aspartate-aminotransferase, and HLA-A) increased, while that of 2 genes (keratin 5 and phosphoglucomutase) decreased in metastatic-tumor-derived cells compared with primary-tumor-derived cells. Of these genes, we assessed the L-plastin gene, an actin-bundling protein, at the protein level using a tissue microarray consisting of 58 clinically stratified colorectal cancer specimens. Consistent with our microarray results, the expression of L-plastin was significantly correlated with the progression of cancer staging. Therefore, our results suggest that the L-plastin gene is a potential metastatic marker. In addition, combining cDNA microarrays and tissue arrays, as shown here, is thought to facilitate the rapid characterization of candidate biomarkers.
authors.affiliation: Biological Technology Laboratory, Helix Research Institute, Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: E J
authors.initials: EJ
authors.lastname: Brown
authors.forename: Y
authors.initials: Y
authors.lastname: Masuho
authors.forename: M
authors.initials: M
authors.lastname: Omata
authors.forename: N
authors.initials: N
authors.lastname: Seki
mesh_terms: D000954:Antigens, Surface / Q000235:genetics; D024442:Aspartate Aminotransferase, Mitochondrial / Q000235:genetics; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000556:secondary*; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D020869:Gene Expression Profiling; D015234:HLA-A Antigens / Q000235:genetics; D006801:Humans; D053555:Keratin-5; D007633:Keratins / Q000235:genetics; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000235:genetics; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D010733:Phosphoglucomutase / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000235:genetics; D018000:Receptors, Peptide / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D015234:HLA-A Antigens; C503940:KRT5 protein, human; D053555:Keratin-5; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C026742:plastin; C074075:TM4SF1 protein, human; D007633:Keratins; D024442:Aspartate Aminotransferase, Mitochondrial; D010733:Phosphoglucomutase