Signal transduction in maize and Arabidopsis mesophyll protoplasts.
Plant protoplasts show physiological perceptions and responses to hormones, metabolites, environmental cues, and pathogen-derived elicitors, similar to cell-autonomous responses in intact tissues and plants. The development of defined protoplast transient expression systems for high-throughput screening and systematic characterization of gene functions has greatly contributed to elucidating plant signal transduction pathways, in combination with genetic, genomic, and transgenic approaches.
Sheen
J
J
Department of Molecular Biology, Massachusetts General Hospital, Wellman 11, 50 Blossom Street, Boston, Massachusetts 02114, USA. sheen@molbio.mgh.harvard.edu
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000378:metabolism*; D015398:Signal Transduction; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism