Two cDNAs coding for histamine-gated ion channels in D. melanogaster.
Histamine, a neurotransmitter and neuroregulatory compound in diverse species, serves as the neurotransmitter of photoreceptors in insects and other arthropods by directly activating a chloride channel. By systematic expression screening of novel putative ligand-gated anion channels, we identified two cDNAs (DM-HisCl-alpha 1 and-alpha 2) coding for putative histamine-gated chloride channels by functional expression in Xenopus laevis oocytes. DM-HisCl-alpha 1 mRNA localizes in the lamina region of the Drosophila eye, supporting the idea that DM-HisCl-alpha 1 may be a neurotransmitter receptor for histamine in the visual system.
Gisselmann
Günter
G
Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany. guenter.gisselmann@ruhr-uni-bochum.de
Pusch
Hermann
H
Hovemann
Bernd T
BT
Hatt
Hanns
H
D000818:Animals; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D006632:Histamine / Q000378:metabolism* / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D017403:In Situ Hybridization; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014982:Xenopus laevis
D018118:Chloride Channels; D018076:DNA, Complementary; D029721:Drosophila Proteins; C446765:HisCl1 protein, Drosophila; D006635:Histamine H2 Antagonists; C446763:ort protein, Drosophila; D002927:Cimetidine; D006632:Histamine