title: Spontaneous renal blood flow autoregulation curves in conscious sinoaortic baroreceptor-denervated rats.
abstract: These experiments examined whether the conscious sinoaortic baroreceptor-denervated (SAD) rat, owing to its high spontaneous arterial pressure (AP) variability, might represent a model for renal blood flow (RBF) autoregulation studies. In eight SAD and six baroreceptor-intact rats, AP and RBF were recorded (1-h periods) before and after furosemide (10 mg/kg followed by 10 mg. kg(-1). h(-1) iv) administration. In control conditions, AP variability was markedly enhanced in SAD rats (coefficient of variation: 16.0 +/- 1.2 vs. 5.4 +/- 0.5% in intact rats), whereas RBF variability was only slightly increased (8.7 +/- 0.6 vs. 6.1 +/- 0.5% in intact rats), suggesting buffering by autoregulatory mechanisms. In SAD rats, but not in intact rats, the AP-RBF relationships could be modeled with a four-parameter sigmoid Weibull equation (r(2) = 0.24 +/- 0.07, 3,600 data pairs/rat), allowing for estimation of an autoregulatory plateau (10.1 +/- 0.7 ml/min) and a lower limit of RBF autoregulation (P(LL) = 93 +/- 6 mmHg, defined as AP at RBF 5% below the plateau). After furosemide treatment, autoregulation curves (r(2) = 0.49 +/- 0.07) in SAD rats were shifted downward (plateau = 8.6 +/- 0.8 ml/min) and rightward (P(LL) = 102 +/- 5 mmHg). In five of six intact rats, P(LL) became measurable (104 +/- 1 mmHg), albeit with limited accuracy (r(2) = 0.09 +/- 0.03). In conclusion, the conscious SAD rat offers the possibility of describing RBF autoregulation curves under dynamic, unforced conditions. The tubuloglomerular feedback and myogenic mechanisms cooperate in setting P(LL) and thus in stabilizing RBF during spontaneous depressor episodes.
authors.affiliation: Centre National de la Recherche Scientifique Unité Mixte de Recherche 5014, Institut Fédératif de Recherche Cardio-vasculaire 39, Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon 69373, France.
authors.forename: Silene L S
authors.initials: SL
authors.lastname: Pires
authors.forename: Claude
authors.initials: C
authors.lastname: Julien
authors.forename: Bruno
authors.initials: B
authors.lastname: Chapuis
authors.forename: Jean
authors.initials: J
authors.lastname: Sassard
authors.forename: Christian
authors.initials: C
authors.lastname: Barrès
mesh_terms: D000818:Animals; D001011:Aorta; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D001835:Body Weight; D003243:Consciousness; D003714:Denervation; D004232:Diuretics / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D011311:Pressoreceptors / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012079:Renal Circulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D004232:Diuretics; D005665:Furosemide