title: Antibodies to hepatitis C virus in patients who have had multiple transfusions in Sri Lanka.
abstract: OBJECTIVE
  To determine the prevalence of antibodies to hepatitis C virus (HCV) in patients who have had multiple transfusions in Sri Lanka.
  
  
  METHODS
  University Medical Unit at the National Hospital, Colombo, the Cancer Institute, Maharagama, and the Lady Ridgeway Children's Hospital, Colombo.
  
  
  METHODS
  One to 5 ml of blood for serology was collected from 200 multiply transfused patients (those who have received five or more blood transfusions).
  
  
  METHODS
  The sera were tested for HCV specific antibodies using a third generation anti-HCV enzyme immuno-assay (EIA) kit. All sera giving positive or intermediate EIA results were re-tested by a commercial HCV Western blot confirmatory test.
  
  
  RESULTS
  Of the 200 patients, 10 (5%) were repeatedly positive and confirmed by the Western blot. 33% (7/21) of haemophiliacs and 10% (3/31) of thalassaemics were positive for antibodies to HCV. Antibodies were not detected in other groups of multiply transfused patients (haemolytic disease, aplastic anemias, chronic renal failure, haematological and other malignancies). Of the 200 patients, those who have had more than 80 blood transfusions had a significantly higher prevalence of antibodies to HCV. The frequency of HCV infection was also higher among those who had received factor concentrates.
  
  
  CONCLUSIONS
  33% of haemophiliacs and 10% of thalassaemics who have received multiple transfusions were infected with HCV. These findings warrant a larger study among blood donors, and justify screening and decontamination of blood and blood products given to haemophiliacs and thalassaemics in Sri Lanka.
authors.affiliation: Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayawardenepura. sirimal@sri.lanka.net
authors.forename: S
authors.initials: S
authors.lastname: Fernando
authors.forename: S S
authors.initials: SS
authors.lastname: Fernando
authors.forename: M H
authors.initials: MH
authors.lastname: Sheriff
authors.forename: U T
authors.initials: UT
authors.lastname: Vitarana
authors.forename: S D
authors.initials: SD
authors.lastname: Fernando
mesh_terms: D001803:Blood Transfusion / Q000706:statistics & numerical data*; D006526:Hepatitis C / Q000097:blood / Q000453:epidemiology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D015995:Prevalence; D013188:Sri Lanka
chemical_list: D018937:Hepatitis C Antibodies