Reduced oviposition of Boophilus microplus feeding on sheep vaccinated with vitellin.
The most abundant protein present in Boophilus microplus eggs, vitellin, was isolated and purified as a non-covalent complex of six glyco-polypeptides of Mr 44-107kDa. The protein complex bound haem. Immuno-blots demonstrated that antibodies raised to vitellin recognised a 200kDa polypeptide in the haemolymph of adult female ticks. This is consistent with the general proposal that in arthropods vitellin is derived by proteolytic processing from a large precursor protein, vitellogenin. In parallel with this study, an 80kDa glycoprotein (GP80) was independently purified from larvae of B. microplus using efficacy in vaccination trials as an assay. Antibodies to GP80 also recognised a 200kDa protein in the haemolymph of ticks and a major 87kDa polypeptide present in the vitellin complex. Conversely, antibodies to purified vitellin recognised GP80. The amino-terminal amino acid sequences of the 87kDa vitellin polypeptide and GP80 were identical for at least the first 11 residues and internal peptide sequences from both polypeptides were co-located in a single but incomplete deduced amino sequence of B. microplus vitellogenin. Thus, GP80 is a processed product from vitellogenin and highly related to but not completely identical with the 87kDa vitellin polypeptide. Vaccination trials in the model host sheep were performed with purified vitellin and GP80. Sheep vaccinated with either purified vitellin or GP80 returned significantly reduced numbers of engorged female ticks with decreased weights and reduced oviposition. In contrast, sheep vaccinated with recombinant hexahis-GP80, which was incorrectly folded and not glycosylated showed no significant effects on ticks. It was concluded that vitellin and GP80 could induce immune responses that partially protect sheep from the tick, B. microplus. However, critical protective epitopes are associated with the folding of the protein and/or the oligosaccharides attached to it.
Tellam
R L
RL
CSIRO Livestock Industries, Molecular Animal Genetics Centre, Gehrmann Research laboratories, Research Road, The University of Queensland, St Lucia 4067, Qld, Australia. ross.tellam@li.csiro.au
Kemp
D
D
Riding
G
G
Briscoe
S
S
Smith
D
D
Sharp
P
P
Irving
D
D
Willadsen
P
P
D000818:Animals; D000906:Antibodies / Q000276:immunology; D004527:Egg Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D026863:Ixodidae / Q000276:immunology / Q000502:physiology*; D010058:Oviposition; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology* / Q000517:prevention & control; D013983:Tick Control / Q000379:methods; D013984:Tick Infestations / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000276:immunology / Q000592:standards
D000906:Antibodies; D004527:Egg Proteins; D006023:Glycoproteins; D014612:Vaccines