Microcin E492 is an unmodified peptide related in structure to colicin V.
The pore-forming microcin E492 was purified by solid-phase extraction and reversed-phase high-pressure liquid chromatography. Its molecular mass was 7,886 Da. The entire 84-amino-acid sequence was determined. There is no postranslational modification in the secreted microcin, and the sequence has homologies with the sequence of the microcin colicin V.
Pons
Anne-Marie
AM
Laboratoire de Spectrométrie de Masse Bioorganique, Université Louis Pasteur, 67000 Strasbourg Cedex, France.
Zorn
Nathalie
N
Vignon
David
D
Delalande
François
F
Van Dorsselaer
Alain
A
Cottenceau
Gilles
G
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003087:Colicins / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011487:Protein Conformation; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003087:Colicins; C020411:microcin