Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis.
Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-gamma by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.
Crocquet-Valdes
P A
PA
Department of Pathology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA.
Díaz-Montero
C M
CM
Feng
H M
HM
Li
H
H
Barrett
A D
AD
Walker
D H
DH
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009161:Mycobacterium / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012284:Rickettsia rickettsii / Q000235:genetics / Q000276:immunology*; D012290:Rickettsial Vaccines / Q000235:genetics / Q000494:pharmacology*; D012373:Rocky Mountain Spotted Fever / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology; D014170:Transformation, Genetic; D019444:Vaccines, DNA / Q000235:genetics / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D012290:Rickettsial Vaccines; D019444:Vaccines, DNA; D007371:Interferon-gamma