title: In vitro studies on the effect of physical cross-linking on the biological performance of aliphatic poly(urethane urea) for blood contact applications.
abstract: The effect of physical cross-linking in candidate cycloaliphatic and hydrophobic poly(urethane urea) (4,4'-methylenebis(cyclohexylisocyanate), H(12)MDI/hydroxy-terminated polybutadiene, HTPBD/hexamethylenediamine, HDA) and poly(ether urethane urea)s (H(12)MDI/HTPBD-PTMG/HDA) on the in vitro calcification and blood-material interaction was studied. All the candidate poly(urethane urea)s and poly(ether urethane urea)s elicit acceptable hemolytic activity, cytocompatibility, calcification, and blood compatibility in vitro. The studies on blood-material interaction reveal that the present poly(urethane urea)s are superior to polystyrene microtiter plates which were used for the studies on blood-material interaction. The present investigation reveals the influence of physical cross-link density on biological interaction differently with poly(urethane urea) and poly(ether urethane urea)s. The higher the physical cross-link density in the poly(urethane urea)s, the higher the calcification and consumption of WBC in whole blood. On the other hand, the higher the physical cross-link density in the poly(ether urethane urea)s, the lesser the calcification and consumption of WBC in whole blood. However a reverse of the above trend has been observed with the platelet consumption in the poly(urethane urea)s and poly(ether urethane urea)s.
authors.affiliation: Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Division, Division of Implant Biology, Biomedical Technology Wing, Thiruvananthapuram 695 012, Kerala, India.
authors.forename: V
authors.initials: V
authors.lastname: Thomas
authors.forename: T V
authors.initials: TV
authors.lastname: Kumari
authors.forename: M
authors.initials: M
authors.lastname: Jayabalan
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000166:cytology; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000166:cytology; D008055:Lipids / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D011140:Polyurethanes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D008055:Lipids; D011091:Polyesters; D011140:Polyurethanes; C044690:polyetherurethane urea