Various indigestible saccharides enhance net calcium transport from the epithelium of the small and large intestine of rats in vitro.
An Ussing chamber technique was used to determine the effects of six indigestible saccharides on net Ca absorption from the luminal side to the basolateral side of isolated preparations of rat jejunal, ileal, cecal and colonic epithelium in vitro. The concentrations of Ca in the Tris buffer solution on the serosal side and on the mucosal side were 1.25 and 10 mmol/L, respectively. After a 30-min incubation, the Ca concentration in the serosal medium was determined and the net transepithelial Ca transport was calculated. The addition of 0.1-200 mmol/L maltitol, difructose anhydride (DFA)III, DFAIV, raffinose, fructooligosaccharide (FOS) or polydextrose (PD) to the mucosal medium increased the net Ca absorption dose-dependently in the jejunum, ileum, cecum and colon preparations. The threshold concentration required to enhance Ca transport and the extent of enhancement of Ca transport differed among the saccharides tested and among the portions of the intestine examined. Among the saccharides tested, DFA IV had the strongest effect on Ca absorption in the jejunum and cecum. We conclude that indigestible carbohydrates directly affect the epithelial tissue and promote Ca absorption in both the small and large intestine in vitro.
Mineo
H
H
Hokkaido Foundation for the Promotion of Scientific and Industrial Technology, Colabo-Hokkaido, Sapporo 001-0021, Japan.
Hara
H
H
Kikuchi
H
H
Sakurai
H
H
Tomita
F
F
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002432:Cecum / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D004063:Digestion; D004848:Epithelium / Q000378:metabolism; D005632:Fructose / Q000494:pharmacology; D005936:Glucans / Q000494:pharmacology; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008320:Maltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D009844:Oligosaccharides / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013402:Sugar Alcohols / Q000494:pharmacology
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D013402:Sugar Alcohols; C116580:fructooligosaccharide; D005632:Fructose; D008320:Maltose; C010745:maltitol; D011887:Raffinose; D002118:Calcium; C033375:polydextrose