Mapping and identification of essential gene functions on the X chromosome of Drosophila.
The Drosophila melanogaster genome consists of four chromosomes that contain 165 Mb of DNA, 120 Mb of which are euchromatic. The two Drosophila Genome Projects, in collaboration with Celera Genomics Systems, have sequenced the genome, complementing the previously established physical and genetic maps. In addition, the Berkeley Drosophila Genome Project has undertaken large-scale functional analysis based on mutagenesis by transposable P element insertions into autosomes. Here, we present a large-scale P element insertion screen for vital gene functions and a BAC tiling map for the X chromosome. A collection of 501 X-chromosomal P element insertion lines was used to map essential genes cytogenetically and to establish short sequence tags (STSs) linking the insertion sites to the genome. The distribution of the P element integration sites, the identified genes and transcription units as well as the expression patterns of the P-element-tagged enhancers is described and discussed.
Peter
Annette
A
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Entwicklungsbiologie, Am Fassberg, 37077 Göttingen, Germany.
Schöttler
Petra
P
Werner
Meike
M
Beinert
Nicole
N
Dowe
Gordon
G
Burkert
Peter
P
Mourkioti
Foteini
F
Dentzer
Lore
L
He
Yuchun
Y
Deak
Peter
P
Benos
Panayiotis V
PV
Gatt
Melanie K
MK
Murphy
Lee
L
Harris
David
D
Barrell
Bart
B
Ferraz
Concepcion
C
Vidal
Sophie
S
Brun
Christine
C
Demaille
Jacques
J
Cadieu
Edouard
E
Dreano
Stephane
S
Gloux
Stephanie
S
Lelaure
Valerie
V
Mottier
Stéphanie
S
Galibert
Francis
F
Borkova
Dana
D
Miñana
Belen
B
Kafatos
Fotis C
FC
Bolshakov
Slava
S
Sidén-Kiamos
Inga
I
Papagiannakis
George
G
Spanos
Lefteris
L
Louis
Christos
C
Madueño
Encarnación
E
de Pablos
Beatriz
B
Modolell
Juan
J
Bucheton
Alain
A
Callister
Debbie
D
Campbell
Lorna
L
Henderson
Nadine S
NS
McMillan
Paul J
PJ
Salles
Cathy
C
Tait
Evelyn
E
Valenti
Phillipe
P
Saunders
Robert D C
RD
Billaud
Alain
A
Pachter
Lior
L
Klapper
Robert
R
Janning
Wilfried
W
Glover
David M
DM
Ashburner
Michael
M
Bellen
Hugo J
HJ
Jäckle
Herbert
H
Schäfer
Ulrich
U
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D015342:DNA Probes; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D020043:Genes, Essential; D017344:Genes, Insect; D008297:Male; D016296:Mutagenesis; D014960:X Chromosome
D015342:DNA Probes; D004251:DNA Transposable Elements