Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea.
A protein designated unguilin was isolated from seeds of the black-eyed pea (Vigna unguiculata). It possesses a molecular weight of 18 kDa and an N-terminal sequence resembling that of cyclophilins and the cyclophilin-like antifungal protein from mung beans, and was adsorbed on Affi-gel blue gel and CM-Sepharose. Unguilin exerted an antifungal effect toward fungi including Coprinus comatus, Mycosphaerella arachidicola, and Botrytis cinerea. In addition, unguilin was capable of inhibiting human immunodeficiency virus-1 reverse transcriptase and the glycohydrolases a- and beta-glucosidases which are involved in HIV infection. Unguilin was devoid of lectin and ribonuclease activities. It inhibited methyl-3H-thymidine uptake by mouse splenocytes and it weakly inhibited translation in a rabbit reticulocyte lysate system. Unguilin resembles mungin in some aspects, but differs from it in others.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin.
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D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002474:Cell-Free System; D021983:Cyclophilins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000737:chemistry*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D011817:Rabbits; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment
D000935:Antifungal Agents; D000998:Antiviral Agents; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; C457580:unguilin protein, Vigna unguiculata; D054303:HIV Reverse Transcriptase; D021983:Cyclophilins