Tramtrack69 interacts with the dMi-2 subunit of the Drosophila NuRD chromatin remodelling complex.
dMi-2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two-hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co-purifies with the dNuRD complex isolated from embryo extracts. dMi-2 and Ttk69 co-immunoprecipitate from embryonic extracts, indicating that they can associate in vivo. Both dMi-2 and Ttk69 co-localize at a number of discrete sites on polytene chromosomes, showing that they bind common target loci. We also demonstrate that dMi-2 and Ttk interact genetically, indicating a functional interaction in vivo. We propose that Ttk69 represses some target genes by remodelling chromatin structure through the recruitment of the dNuRD complex.
Murawsky
C M
CM
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Brehm
A
A
Badenhorst
P
P
Lowe
N
N
Becker
P B
PB
Travers
A A
AA
D000251:Adenosine Triphosphatases; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D006655:Histone Deacetylases / Q000737:chemistry* / Q000378:metabolism*; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D011485:Protein Binding; D021122:Protein Subunits; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D001324:Autoantigens; D002352:Carrier Proteins; D002843:Chromatin; D029721:Drosophila Proteins; C116095:Mi-2 protein, Drosophila; D021122:Protein Subunits; D012097:Repressor Proteins; C075878:ttk protein, Drosophila; D006655:Histone Deacetylases; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D000251:Adenosine Triphosphatases