Genetic ablation of the t-SNARE SNAP-25 distinguishes mechanisms of neuroexocytosis.
Axon outgrowth during development and neurotransmitter release depends on exocytotic mechanisms, although what protein machinery is common to or differentiates these processes remains unclear. Here we show that the neural t-SNARE (target-membrane-associated-soluble N-ethylmaleimide fusion protein attachment protein (SNAP) receptor) SNAP-25 is not required for nerve growth or stimulus-independent neurotransmitter release, but is essential for evoked synaptic transmission at neuromuscular junctions and central synapses. These results demonstrate that the development of neurotransmission requires the recruitment of a specialized SNARE core complex to meet the demands of regulated exocytosis.
Washbourne
Philip
P
Department of Neurosciences, University of New Mexico Health Science Center, Albuquerque, New Mexico 87131, USA.
Thompson
Peter M
PM
Carta
Mario
M
Costa
Edmar T
ET
Mathews
James R
JR
Lopez-Benditó
Guillermina
G
Molnár
Zoltán
Z
Becher
Mark W
MW
Valenzuela
C Fernando
CF
Partridge
L Donald
LD
Wilson
Michael C
MC
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D003964:Diaphragm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D005089:Exocytosis / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000502:physiology; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D050600:SNARE Proteins; D009435:Synaptic Transmission / Q000502:physiology*; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050600:SNARE Proteins; C494209:Snap25 protein, mouse; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins