Enhancement of the steady-state magnetization in TROSY experiments.
Under the condition that the longitudinal relaxation time of spin I is shorter than the longitudinal relaxation time of spin S the steady-state magnetization in [S,I]-TROSY-type experiments can be enhanced by intermediate storage of a part of the steady-state magnetization of spin I on spin S with a pulse sequence element during the relaxation delay. It is demonstrated with samples ranging in size from the 1 kDa cyclosporin to the 110 kDa 15N,2H-labeled dihydroneopterin Aldolase that intermediate storage of steady-state magnetization in a [15N,1H]-TROSY experiment yields a signal gain of 10-25%. The method proposed here for intermediate storage of steady-state magnetization can be implemented in any [15N,1H]-TROSY-type experiments.
Riek
R
R
Institut für Molekularbiologie und Biophysik Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland. rr@mol.biol.ethz.ch
D000446:Aldehyde-Lyases / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D016572:Cyclosporine / Q000737:chemistry; D008280:Magnetics; D008962:Models, Theoretical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D013211:Staphylococcus aureus / Q000201:enzymology
D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins; D016572:Cyclosporine; D000446:Aldehyde-Lyases; C020044:dihydroneopterin aldolase