Effects of low-intensity pulsed ultrasound on proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen gel.
The effects of low-intensity pulsed ultrasound (US) on the proliferation and chondroitin sulfate synthesis of cultured chondrocytes embedded in Atelocollagen gel in vitro were examined. Articular cartilage was harvested from the hip, knee, and shoulder joints of 10-week-old Japanese white rabbits. Chondrocytes isolated by collagenase digestion were embedded in type I collagen gel, Atelocollagen gel, and were cultured in Dulbecco's modified eagle's medium for 3 weeks. The US apparatus, SAFHS, was used to deliver an ultrasound signal with spatial and temporal average intensities of 30 mW/cm(2) (US group). The frequency was 1.5 MHz with a 200-microsecond tone burst repeated at 1.0 kHz. US treatments were administered for 20 min per day under culture dishes, with the medium replaced twice a week. Another group of cells was exposed to sham ultrasound as a control. Cell number, histological findings, synthesis of isomers of chondroitin sulfate, and stiffness of the chondrocyte-collagen gel composites were analyzed. US exposure promoted synthesis of chondroitin sulfate, especially chondroitin 6-sulfate, although it did not significantly enhance cell number and stiffness. In this three-dimensional culture model, these results suggest that US exposure may be clinically useful in improving the quality of chondrocyte-Atelocollagen implants for transplantation into articular cartilage defects.
Nishikori
T
T
Department of Orthopaedics, Shimane Medical University, 89-1 Enya-cho, Izumo-shi, Shimane-ken 693-8501, Japan. t-nskr@shimane-med.ac.jp
Ochi
M
M
Uchio
Y
Y
Maniwa
S
S
Kataoka
H
H
Kawasaki
K
K
Katsube
K
K
Kuriwaka
M
M
D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D002455:Cell Division; D002478:Cells, Cultured; D018914:Cells, Immobilized; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000096:biosynthesis*; D003094:Collagen; D003470:Culture Media; D017102:Fracture Healing; D005782:Gels; D008422:Materials Testing; D011817:Rabbits; D014464:Ultrasonic Therapy; D014465:Ultrasonics
D001672:Biocompatible Materials; D003470:Culture Media; D005782:Gels; C059821:atelocollagen; D002809:Chondroitin Sulfates; D003094:Collagen