title: Behavior of plastid nucleoids during male gametogenesis in Plumbago auriculata.
abstract: We characterized the behavior of plastid (pt) and mitochondrial (mt) nucleoids during male gametogenesis in Plumbago auriculata in three dimensions. The behavior of pt-nucleoids and mt-nucleoids differed throughout male gametogenesis. Pt-nucleoids were distributed in a characteristic manner in three stages: in the early microspore, pt-nucleoids assemble around cell nucleus; in the mid-generative cell, pt-nucleoids gather at the internal side of the pollen; in the late-generative cell, pt-nucleoids aggregation turns its pole to the external side of the pollen. We also studied organelle nucleoids in the egg and the central cell by a method in which semi-thick sections of resin-embedded anthers and ovaries were observed by confocal laser scanning microscopy. The number of pt-nucleoids in the sperm cell did not differ significantly from that in the egg. These results suggest that the behavior of DNA-containing organelles is regulated strictly during male gametogenesis in P. auriculata, and that a biparental inheritance of plastids in the Plumbago embryo is more favored than was previously thought.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Nagata
authors.forename: A
authors.initials: A
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Kuroiwa
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D017403:In Situ Hybridization; D019684:Magnoliopsida / Q000235:genetics / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D008954:Models, Biological; D018514:Plant Structures / Q000166:cytology; D018087:Plastids / Q000378:metabolism* / Q000648:ultrastructure; D011058:Pollen / Q000648:ultrastructure; D012098:Reproduction
chemical_list: D018744:DNA, Plant