Fructose-1,6-diphosphate-added total parenteral nutrition in septic animals and stressed patients.
OBJECTIVE
To investigate the roles of fructose-1,6-diphosphate (FDP)-added total parenteral nutrition (TPN) in septic animals and stressed patients.


METHODS
Thirteen adult dogs were randomly assigned to one of two study groups 6 hours after the induction of severe intra-abdominal infection. Group TPN (n = 6) received 70 kcal.kg-1.d-1 of nonprotein calorie (NPC) and 0.56 g.kg-1.d-1 of nitrogen. 1 g/kg of FDP was also infused to the animals in group TPN + FDP (n = 7) everyday. In the clinical study, the control group received routine TPN, while the study group (n = 16) was treated with TPN plus FDP (5 g, two times a day) for 7 days.


RESULTS
In dogs with TPN support, plasma ATP levels were not changed significantly, while the value in the TPN + FDP group increased significantly from 0.18 mumol/L to 0.46 mumol/L at 24 h and 0.51 mumol/L at 48 h (P < 0.01). Muscular ATP increased markedly in the TPN + FDP group. Muscular creatine phosphate values were not significantly changed in the TPN group, but the values increased in the TPN + FDP group from 4.06 mumol/g.wt at the beginning to 4.93 mumol/g.wt at 24 h and 5.60 mumol/g.wt at 48 h (P < 0.05), with a cytochrome oxidase increase in immunohistochemistry stain. In the clinical study, plasma ATP levels increased and urinary 3-methylhistidine production significantly decreased with an improved value for positive accumulative nitrogen balance in the FDP-infused group.


CONCLUSIONS
Our results suggest that total parenteral nutrition support with the supplement of fructose-1,6-diphosphate has a positive role in body energy production and protein metabolism in septic animals and stressed patients.
Shu
Z
Z
Department of General Surgery, Shanghai Traditional Chinese Medicine and Western Medicine, Integrated Hospital, Shanghai 200082, China. shuhan@online.sh.cn
Li
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D000818:Animals; D003422:Critical Care; D004285:Dogs; D004734:Energy Metabolism / Q000187:drug effects*; D005635:Fructosediphosphates / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D010289:Parenteral Nutrition, Total; D011506:Proteins / Q000378:metabolism*; D018805:Sepsis / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*
D005635:Fructosediphosphates; D011506:Proteins; D000255:Adenosine Triphosphate; C029063:fructose-1,6-diphosphate