Isolation and properties of stachyrase A, a chymotrypsin-like serine proteinase from Stachybotrys chartarum.
A strain of the common mold Stachybotrys chartarum has been isolated from the lung of a child with pulmonary hemorrhage. We report the purification of stachyrase A, a new serine chymotrypsin-like proteinase from S. chartarum. This enzyme cleaves major protease inhibitors, several biologically active peptides, and collagen, all of which are found in the lung.
Kordula
Tomasz
T
Department of Biological, Geological and Environmental Sciences, Cleveland State University, Cleveland, Ohio 44115, USA.
Banbula
Agnieszka
A
Macomson
Jeremy
J
Travis
James
J
D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D001665:Binding Sites; D001920:Bradykinin / Q000378:metabolism; D002918:Chymotrypsin / Q000302:isolation & purification / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D009496:Neurotensin / Q000378:metabolism; D012697:Serine Endopeptidases; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D013191:Stachybotrys / Q000201:enzymology*; D013373:Substance P / Q000378:metabolism; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology; D000514:alpha 1-Antichymotrypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D015842:Serine Proteinase Inhibitors; D000514:alpha 1-Antichymotrypsin; D000511:alpha-Macroglobulins; D000804:Angiotensin II; D013373:Substance P; D009496:Neurotensin; D014108:Tosylphenylalanyl Chloromethyl Ketone; D003094:Collagen; D004549:Elastin; D000803:Angiotensin I; D012697:Serine Endopeptidases; C443559:stachyrase A; D002918:Chymotrypsin; D001920:Bradykinin