Production and characterisation of monoclonal antibodies specific for chicken interleukin-2.
Using genetic immunisation of mice, we produced antibodies against chicken interleukin-2 (ChIL-2), the first produced against a non-mammalian interleukin. After a final injection with a recombinant ChIL-2 protein, two stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of the two MAbs to recombinant ChIL-2 produced by Escherichia coli and COS-7 cells was demonstrated in an indirect ELISA, Western blotting and dot blots. Both of them were able to neutralise the biological activity of the ChIL-2, but neither allowed the detection of ChIL-2 by flow cytometry.
Rothwell
L
L
Institute for Animal Health, Compton, Berkshire RG20 7NN, UK. lisa.rothwell@bbsrc.ac.uk
Hamblin
A
A
Kaiser
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D000918:Antibody Specificity; D015153:Blotting, Western / Q000662:veterinary; D019556:COS Cells; D002645:Chickens / Q000276:immunology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D005434:Flow Cytometry / Q000662:veterinary; D007376:Interleukin-2 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000662:veterinary; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D011994:Recombinant Proteins