A phospholipase C inhibitor, phenylmethylsulfonyl fluoride, ameliorates ischemic injury to brain mitochondria in rats.
OBJECTIVE
To study the effect of phenylmethylsulfonyl fluoride (PMSF), a phospholipase C inhibitor, on ischemic injury to brain mitochondria in rats.


METHODS
The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria were measured. The effect of phenylmethylsulfonyl fluoride was tested.


RESULTS
The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria in ischemia group decreased but increased in the PMSF treatment group (P < 0.05).


CONCLUSIONS
PMSF ameliorated ischemic injury to brain mitochondria in rats.
Wang
Q S
QS
Department of Neurobiology, Life Science School, University of Science and Technology of China, Hefei 230027, China. wqss@china.com
Zhou
J N
JN
Xu
T L
TL
Li
L S
LS
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D010743:Phospholipids; D010664:Phenylmethylsulfonyl Fluoride; D010738:Type C Phospholipases