Identification, cloning, and developmental expression of hepatoma-derived growth factor in the developing rat heart.
Hepatoma derived growth factor (HDGF) was identified as a developmentally regulated cardiac gene by mRNA differential display using 12-day rat fetal conotruncus vs. newborn aorta. The full-length rat HDGF cDNA was cloned from a rat fetal heart cDNA library and found to be 94 and 88% homologous to the mouse and human sequence, respectively. The rat sequence, like the human and mouse, contains a highly conserved amino portion and putative bipartite nuclear localization sequence. By Northern analysis, HDGF is highly expressed in the fetal conotruncus, heart, kidney, brain, and gut. By immunocytochemistry, HDGF was first detected only in atrial myocytes, hind gut epithelia, and notochord of the E10 rat with a nuclear expression pattern. By E12, expression had broadened to include the ventricular myocytes, endocardial cells, and cells of the ventricular outflow tract. HDGF is unique in that it is the first described nuclear targeted growth factor in the developing heart. The early expression of HDGF in embryonic heart and fetal gut suggests that HDGF may play a role in cardiovascular growth and differentiation.
Everett
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AD
Department of Pediatrics, Division of Pediatric Cardiology and the Cardiovascular Research Center of the University of Virginia Health System, Charlottesville, Virginia 22908-1356, USA. ade5r@virginia.edu
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D002319:Cardiovascular System / Q000166:cytology / Q000196:embryology; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000196:embryology*; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D051381:Rats / Q000196:embryology*; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C051610:hepatoma-derived growth factor