Cellular signaling, AGE accumulation and gene expression in hepatocytes of lean aging rats fed ad libitum or food-restricted.
The effects of food restriction on liver glucagon and vasopressin V1a receptors, on AGE accumulation and on gene expression were investigated in 10- and 30-month-old WAG/Rij female rats fed ad libitum or chronically food-restricted by 30%. The age-related increase in glucagon and vasopressin V1a receptor density, as well as the rise in glucagon-induced cAMP generation was prevented by the restriction. AGE accumulation, characteristic of the aging process, was normalized in food-restricted animals. Gene expression determined with rat Atlas cDNA Expression Arrays containing 1176 cDNA indicates that a few genes exhibited a greater than twofold change in mRNA ratios with age. Most down-regulated genes were related to oxidative metabolism of lipids, and most of the up-regulated genes were concerned with the cell cycle and transcription factors. Chronic food restriction partially prevents these changes in gene expression and induces up- and down-regulation of several mRNAs which are not modified with age in ad libitum fed rats.
Teillet
L
L
Hôpital Sainte-Périne, Assistance Publique-Hôpitaux de Paris (AP-HP), 75016, Paris, France.
Ribière
P
P
Gouraud
S
S
Bakala
H
H
Corman
B
B
D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000804:Angiotensin II / Q000235:genetics; D000818:Animals; D001835:Body Weight; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D015870:Gene Expression; D005934:Glucagon / Q000235:genetics; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D014667:Vasopressins / Q000235:genetics
D017127:Glycation End Products, Advanced; D014667:Vasopressins; D000804:Angiotensin II; D005934:Glucagon; D000242:Cyclic AMP