title: Preoperative oral B vitamins prevent nitrous oxide-induced postoperative plasma homocysteine increases.
abstract: Nitrous oxide increases total homocysteine (tHcy) plasma levels, which are associated with an increase in perioperative myocardial ischemia. We designed this study to determine whether oral B vitamins, which are cofactors in homocysteine metabolism, can prevent nitrous oxide anesthesia-induced tHcy increases in patients undergoing elective surgery scheduled to last longer than 3 h. Fifty-three patients presenting for elective revision knee or hip arthroplasty received in random, double-blinded fashion oral vitamin B complex (folate 2.5 mg, B(6) 25 mg, and B(12) 500 microg) or placebo daily for 1 wk before surgery. Anesthesia was induced with propofol and maintained with an opioid, isoflurane, and nitrous oxide/oxygen (inspired nitrous oxide >50%). Blood samples for measurement of tHcy concentration were obtained at study enrollment, before induction, on arrival in the postanesthesia care unit, and on Day 5. Fourteen patients had their surgery rescheduled after taking their vitamins and were removed from the study. The Placebo group had a mean increase in tHcy concentration from baseline of 15% +/- 31% compared with the Vitamin group, which had an initial decrease of 9.1% +/- 11% (P = 0.035). This was maintained throughout the 5-day study period. The use of an oral B vitamin complex prevented the increase in postoperative tHcy by nitrous oxide.
  
  
  CONCLUSIONS
  The use of nitrous oxide anesthesia increases postoperative homocysteine concentrations and associated myocardial ischemia. This study indicates that a 1-wk course of oral B vitamins can prevent the increase in homocysteine from nitrous oxide, and, by implication, myocardial ischemia as well.
authors.affiliation: Department of Anesthesiology, Clinical Pharmacology, University of Western Ontario, London, Ontario, Canada. nbadner@uwo.ca
authors.forename: N H
authors.initials: NH
authors.lastname: Badner
authors.forename: D
authors.initials: D
authors.lastname: Freeman
authors.forename: J D
authors.initials: JD
authors.lastname: Spence
mesh_terms: D000284:Administration, Oral; D000368:Aged; D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D019643:Arthroplasty, Replacement; D004311:Double-Blind Method; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D008297:Male; D009609:Nitrous Oxide / Q000009:adverse effects*; D011300:Preoperative Care; D011446:Prospective Studies; D012086:Reoperation; D016037:Single-Blind Method; D014805:Vitamin B 12 / Q000008:administration & dosage*; D025101:Vitamin B 6 / Q000008:administration & dosage*
chemical_list: D018685:Anesthetics, Inhalation; D006710:Homocysteine; D025101:Vitamin B 6; D009609:Nitrous Oxide; D014805:Vitamin B 12