Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells.
A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non-haem iron. Using human intestinal Caco-2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration-dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron.
Sharp
Paul
P
Centre for Nutrition and Food Safety, School of Biomedical and Life Sciences, University of Surrey, Guildford GU2 7XH, UK. p.sharp@surrey.ac.uk
Tandy
Sarah
S
Yamaji
Sachie
S
Tennant
Jason
J
Williams
Mark
M
Singh Srai
Surjit Kaila
SK
D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000378:metabolism; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D002413:Cations, Divalent; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects*; D006418:Heme; D006801:Humans; D007422:Intestines / Q000166:cytology; D007501:Iron / Q000493:pharmacokinetics*; D033862:Iron-Binding Proteins; D008670:Metals / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011990:Receptors, Transferrin / Q000378:metabolism; D013997:Time Factors; D000099236:Solute Carrier Family 11, Member 2
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D002413:Cations, Divalent; D006418:Heme; D007501:Iron; D033862:Iron-Binding Proteins; D008670:Metals; D008840:Microfilament Proteins; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D000099236:Solute Carrier Family 11, Member 2; C026615:villin