title: Comparative genomic hybridization analysis of radiation-associated and sporadic meningiomas.
abstract: Ionizing irradiation to the skull is a known risk factor for meningioma development. To gain insight into the molecular mechanisms that underlie radiation-associated meningioma (RAM), we characterized the somatic genetic alterations in 16 RAMs by using comparative genomic hybridization and compared the pattern of alterations with 17 nonradiation-associated meningiomas (non-RAM). Most tumors (29/33;87.9%) displayed at least one DNA copy number alteration, and 11 out of 33 (33%) exhibited four or more changes. The mean number of DNA copy number changes was similar in RAMs (2.4+/-1.9) and in non-RAMs (2.5+/-1.9). The most common DNA losses were noted in chromosome 22 (56.2% in RAM, and 47% in non-RAM) and chromosome 1 (37.5% in RAM and 35.3% in non-RAM), with no significant differences between the two groups. Noteworthy, gain in DNA copy number of chromosomes 8 and 12 was detected in two RAM tumors only. In conclusion, no significant differences were noted between RAMs and non-RAMs regarding the number of genetic changes and the extent and frequency of chromosomes 1 and 22 losses. These preliminary data suggest that the tumorogenic pathways of meningioma formation are similar, regardless of previous skull irradiation.
authors.affiliation: Danek Gertner Institute of Genetics, Chaim Sheba Medical Center, 52621, Tel-Hashomer, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Rienstein
authors.forename: D
authors.initials: D
authors.lastname: Loven
authors.forename: O
authors.initials: O
authors.lastname: Israeli
authors.forename: Z
authors.initials: Z
authors.lastname: Ram
authors.forename: Z H
authors.initials: ZH
authors.lastname: Rappaport
authors.forename: G
authors.initials: G
authors.lastname: Barkai
authors.forename: B
authors.initials: B
authors.lastname: Goldman
authors.forename: A
authors.initials: A
authors.lastname: Aviram-Goldring
authors.forename: E
authors.initials: E
authors.lastname: Friedman
mesh_terms: D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D002892:Chromosomes, Human, Pair 22; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D009693:Nucleic Acid Hybridization