Isolation of X and Y chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis.
The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.
Fujisawa
M
M
Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
Hayashi
K
K
Nishio
T
T
Bando
T
T
Okada
S
S
Yamato
K T
KT
Fukuzawa
H
H
Ohyama
K
K
D002875:Chromosomes / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005819:Genetic Markers