title: Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response.
abstract: Reactive oxygen intermediates (ROI) are strongly associated with plant defense responses. The origin of these ROI has been controversial. Arabidopsis respiratory burst oxidase homologues (rboh genes) have been proposed to play a role in ROI generation. We analyzed lines carrying dSpm insertions in the highly expressed AtrbohD and AtrbohF genes. Both are required for full ROI production observed during incompatible interactions with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000(avrRpm1) and the oomycete parasite Peronospora parasitica. We also observed reduced cell death, visualized by trypan blue stain and reduced electrolyte leakage, in the Atrboh mutants after DC3000(avrRpm1) inoculation. However, enhanced cell death is observed after infection of mutant lines with P. parasitica. Paradoxically, although atrbohD mutation eliminated the majority of total ROI production, atrbohF mutation exhibited the strongest effect on cell death.
authors.affiliation: Department of Biology and Curriculum in Genetics and Molecular Biology, CB 3280, 108 Coker Hall, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Torres
authors.forename: Jeffery L
authors.initials: JL
authors.lastname: Dangl
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology*; D016923:Cell Death; D006861:Hydrogen Peroxide / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology*; D008957:Models, Genetic; D016296:Mutagenesis; D009154:Mutation; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D009569:Nitric Oxide / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D018521:Plant Physiological Phenomena; D017382:Reactive Oxygen Species; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D006861:Hydrogen Peroxide; D008562:Membrane Glycoproteins; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D009569:Nitric Oxide; D017382:Reactive Oxygen Species; C056558:CYBB protein, human; C000589475:RBOH F protein, Arabidopsis