Upregulation of acetylcholine synthesis by bone morphogenetic protein 9 in a murine septal cell line.
Previous studies showed that bone morphogenetic protein 9 (BMP-9) induces the expression of choline acetyltransferase and the vesicular acetylcholine (ACh) transporter, and upregulates ACh synthesis in cultured primary neurons from embryonic mouse septum [I. López-Coviella, B. Berse, R. Krauss, R.S. Thies, J.K. Blusztajn, Induction and maintenance of the neuronal cholinergic phenotype in the central nervous system by BMP-9. Science 289 (2000) 313-316]. In the present studies we investigated the effects of BMP-9 on ACh synthesis in the cholinergic mouse SN56T17 septal cell line. BMP-9 increased ACh synthesis in these cells up to 2.5-fold in a time- and dose-dependent, saturable manner. The maximal effect of BMP-9 was observed after a 3-day treatment and the median effective concentration of BMP-9 was 0.5 ng/ml. These data show that SN56T17 cells are a useful model for studies of the effects of BMPs on the cholinergic phenotype.
López-Coviella
Ignacio
I
Department of Psychiatry, Boston University School of Medicine, 85 East Newton Street, Room M1009, Boston, MA02118, USA.
Berse
Brygida
B
Thies
R Scott
RS
Blusztajn
Jan Krzysztof
JK
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D055427:Growth Differentiation Factor 2; D051379:Mice; D009994:Osmolar Concentration; D012688:Septum Pellucidum / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
D019485:Bone Morphogenetic Proteins; C526108:Gdf2 protein, mouse; D055427:Growth Differentiation Factor 2; D000109:Acetylcholine