title: The [URE3] phenotype: evidence for a soluble prion in yeast.
abstract: The aggregation of the two yeast proteins Sup35p and Ure2p is widely accepted as a model for explaining the prion propagation of the phenotypes [PSI+] and [URE3], respectively. Here, we demonstrate that the propagation of [URE3] cannot simply be the consequence of generating large aggregates of Ure2p, because such aggregation can be found in some conditions that are not related to the prion state of Ure2p. A comparison of [PSI+] and [URE3] aggregation demonstrates differences between these two prion mechanisms. Our findings lead us to propose a new unifying model for yeast prion propagation.
authors.affiliation: Centre de Génétique Moléculaire, 91190 Gif-sur-Yvette, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Fernandez-Bellot
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Guillemet
authors.forename: Frederique
authors.initials: F
authors.lastname: Ness
authors.forename: Agnes
authors.initials: A
authors.lastname: Baudin-Baillieu
authors.forename: Leslie
authors.initials: L
authors.lastname: Ripaud
authors.forename: Mick
authors.initials: M
authors.lastname: Tuite
authors.forename: Christophe
authors.initials: C
authors.lastname: Cullin
mesh_terms: D000595:Amino Acid Sequence; D005979:Glutathione Peroxidase; D008969:Molecular Sequence Data; D010641:Phenotype; D011328:Prions / Q000737:chemistry* / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D012995:Solubility
chemical_list: D011328:Prions; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae