title: Molecular analysis of single colonies reveals a diverse origin of initial clonal proliferation in B-precursor acute lymphoblastic leukemia that can precede the t(12;21) translocation.
abstract: The pathogenesis of pediatric B-precursor acute lymphoblastic leukemia is largely unknown, and even with nonrandom chromosomal translocations present, the precise order of clonal molecular events is undefined. We developed an in vitro system using cytokines interleukin (IL)-3, IL-7, IL-10, and FMS-like tyrosine kinase 3 ligand with CD40 ligand-expressing fibroblasts to obtain single blast colonies from which clonal immunoglobulin heavy chain (IgH), T-cell receptor delta gene rearrangements, and, in t(12;21)-positive cases, TEL-AML1 fusion transcripts could be simultaneously PCR amplified. The proliferation of early tumor progenitors increased subclone detection enabling us, in seven diagnostic samples, to determine the stage of differentiation at which each leukemia occurred. Four were derived from the stage before initiation of IgH rearrangement, one during recombination of variable, joining, and diversity segments of the heavy chain gene VDJ(H), and two after completion of IgH rearrangement. Furthermore, analysis of a t(12;21)-positive leukemia with unusually late onset, identified both TEL-AML1-positive and -negative colonies carrying a clonal T-cell receptor delta rearrangement, inferring the presence of clonal expansion before the occurrence of the t(12;21). In contrast, in a typical, early onset t(12;21)-positive leukemia, the t(12;21) appeared to be the first clonal event. In both leukemias, the t(12;21) occurred before recombination of variable, joining and diversity segments of the heavy chain gene VDJ.
authors.affiliation: CRC Institute for Cancer Studies, University of Birmingham, United Kingdom.
authors.forename: V J
authors.initials: VJ
authors.lastname: Weston
authors.forename: C M
authors.initials: CM
authors.lastname: McConville
authors.forename: J R
authors.initials: JR
authors.lastname: Mann
authors.forename: P J
authors.initials: PJ
authors.lastname: Darbyshire
authors.forename: S
authors.initials: S
authors.lastname: Lawson
authors.forename: J
authors.initials: J
authors.lastname: Gordon
authors.forename: P A
authors.initials: PA
authors.lastname: Moss
authors.forename: A M
authors.initials: AM
authors.lastname: Taylor
authors.forename: T
authors.initials: T
authors.lastname: Stankovic
mesh_terms: D001402:B-Lymphocytes / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002881:Chromosomes, Human, Pair 12; D002891:Chromosomes, Human, Pair 21; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006801:Humans; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic