Real-time single-molecule imaging of the infection pathway of an adeno-associated virus.
We describe a method, based on single-molecule imaging, that allows the real-time visualization of the infection pathway of single viruses in living cells, each labeled with only one fluorescent dye molecule. The tracking of single viruses removes ensemble averaging. Diffusion trajectories with high spatial and time resolution show various modes of motion of adeno-associated viruses (AAV) during their infection pathway into living HeLa cells: (i) consecutive virus touching at the cell surface and fast endocytosis; (ii) free and anomalous diffusion of the endosome and the virus in the cytoplasm and the nucleus; and (iii) directed motion by motor proteins in the cytoplasm and in nuclear tubular structures. The real-time visualization of the infection pathway of single AAVs shows a much faster infection than was generally observed so far.
Seisenberger
G
G
Department Chemie, Ludwig-Maximilians-Universität München, Butenandtstrasse 11, D-81377 München, Germany.
Ried
M U
MU
Endress
T
T
Büning
H
H
Hallek
M
M
Bräuchle
C
C
D000327:Adsorption; D001692:Biological Transport; D002467:Cell Nucleus / Q000821:virology*; D002470:Cell Survival; D003593:Cytoplasm / Q000821:virology*; D000229:Dependovirus / Q000502:physiology*; D004058:Diffusion; D004705:Endocytosis; D011992:Endosomes / Q000821:virology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism; D005822:Genetic Vectors / Q000502:physiology; D006367:HeLa Cells; D006790:Host-Parasite Interactions / Q000502:physiology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D020409:Molecular Motor Proteins / Q000378:metabolism; D009068:Movement; D015739:Nocodazole / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D005456:Fluorescent Dyes; D020409:Molecular Motor Proteins; D015739:Nocodazole