title: Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus.
abstract: The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
authors.affiliation: Department of Pharmacology, The Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Monk
authors.forename: K
authors.initials: K
authors.lastname: Desai
authors.forename: C A
authors.initials: CA
authors.lastname: Brady
authors.forename: J M
authors.initials: JM
authors.lastname: Williams
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: A
authors.initials: A
authors.lastname: Princivalle
authors.forename: A G
authors.initials: AG
authors.lastname: Hope
authors.forename: N M
authors.initials: NM
authors.lastname: Barnes
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D006624:Hippocampus / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D011985:Receptors, Serotonin / Q000276:immunology* / Q000378:metabolism*; D044406:Receptors, Serotonin, 5-HT3
chemical_list: D000906:Antibodies; D007106:Immune Sera; D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3