title: Response of Tribolium castaneum (Coleoptera, Tenebrionidae) to Salpichroa origanifolia Withanolides.
abstract: Biological effects on Tribolium castaneum larvae were evaluated for three withanolides isolated from Salpichroa origanifolia (Solanaceae), (20S,22R,24S,25S,26R)-5alpha,6alpha:22,26:24,25-triepoxy-26-hydroxy-17(13-->18)-abeo-ergosta-2,13,15,17-tetraen-1-one (salpichrolide A, 1), (20S,22R,24S,25S,26R)-22,26:24,25-diepoxy-5alpha,6beta,26-trihydroxy-17(13-->18)-abeo-ergosta-2,13,15,17-tetraen-1-one (salpichrolide C, 2), and (20S,22R,24S,25S,26R)-5alpha,6alpha:22,26:24,25-triepoxy-15,26-dihydroxy-17(13-->18)-abeo-ergosta-2,13,15,17-tetraen-1-one (salpichrolide G, 3), and for several chemically modified analogues. The compounds were incorporated into the larval diet at concentrations of 500 and 2000 ppm. Salpichrolide C (2) produced a significant delay in the development of neonate larvae to adults at the highest concentration (2000 ppm); development delays and lethal effects were produced by salpichrolides A (1) and G (3) at both concentrations assayed. The size of surviving adults was used as a criterion for assessing feedant deterrent effects; the results suggest that these compounds act as feeding inhibitors. Influence of chemical modifications in development delay was analyzed.
authors.affiliation: Cátedra de Zoología Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Avenida San Martín 4453, (1417) Buenos Aires, Argentina.
authors.forename: Graciela
authors.initials: G
authors.lastname: Mareggiani
authors.forename: María I
authors.initials: MI
authors.lastname: Picollo
authors.forename: Adriana S
authors.initials: AS
authors.lastname: Veleiro
authors.forename: María C
authors.initials: MC
authors.lastname: Tettamanzi
authors.forename: Miriam O V
authors.initials: MO
authors.lastname: Benedetti-Doctorovich
authors.forename: Gerardo
authors.initials: G
authors.lastname: Burton
authors.forename: Eduardo
authors.initials: E
authors.lastname: Zerba
mesh_terms: D000818:Animals; D001681:Biological Assay; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004875:Ergosterol / Q000031:analogs & derivatives; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019657:Solanaceae / Q000737:chemistry*; D014231:Tribolium / Q000187:drug effects* / Q000254:growth & development
chemical_list: D004852:Epoxy Compounds; D007306:Insecticides; C453519:salpichrolide A; C453502:salpichrolide C; C453501:salpichrolide G; D004875:Ergosterol