title: PCO(2) in the large intestine of mice, rats, guinea pigs, and dogs and effects of the dietary substrate.
abstract: PCO(2) in the lumen and serosa of cecum and colon was measured in rats, guinea pigs, and dogs to examine the relationship between serosal PCO(2) and the incidence of intestinal necrotic lesions after administration of gas-carrier contrast agents in rodents. The effects of the dietary substrate were tested in a group of mice maintained on a diet based on glucose as the only carbohydrate source. The anesthetic used was a fentanyl-fluanison-midazolam mixture (rodents) and pentobarbital (dogs). PCO(2) was measured in vivo and postmortem, and the kinetics of the postmortem serosal PCO(2) [transmural CO(2) flux (J(CO(2)))] was calculated. PCO(2) in the cecal serosa and lumen, respectively, was 64 +/- 4 and 392 +/- 18 Torr in rats, 67 +/- 3 and 276 +/- 17 Torr in guinea pigs, and 73 +/- 6 and 137 +/- 7 Torr in mice on glucose-based diet. In the colon serosa and lumen of dogs, PCO(2) was 30 +/- 6 and 523 +/- 67 Torr, respectively. Serosal PCO(2) increased rapidly after death in rats and slower in guinea pigs and mice, and the slowest change was observed in dogs. Compared with dogs, serosal PCO(2) and J(CO(2)) of rats and guinea pigs were significantly higher. Serosal PCO(2) of guinea pigs was similar to that of rats, whereas the J(CO(2)) of guinea pigs was significantly lower. These data suggest a causal relationship between the ability of the cecal and colonic wall to act as a barrier to CO(2) diffusion and the presence of characteristic gas-carrier contrast agent-induced intestinal lesions in mice and rats and their absence in guinea pigs, dogs, and other species.
authors.affiliation: Research and Development, Amersham Health AS, N-0401 Oslo, Norway. henrik.rasmussen@amersham.com
authors.forename: Henrik
authors.initials: H
authors.lastname: Rasmussen
authors.forename: Peyman
authors.initials: P
authors.lastname: Mirtaheri
authors.forename: Hubert
authors.initials: H
authors.lastname: Dirven
authors.forename: Helge
authors.initials: H
authors.lastname: Johnsen
authors.forename: Gunnvald
authors.initials: G
authors.lastname: Kvarstein
authors.forename: Tor Inge
authors.initials: TI
authors.lastname: Tønnessen
authors.forename: Tore
authors.initials: T
authors.lastname: Midtvedt
mesh_terms: D000818:Animals; D002138:Calibration; D002245:Carbon Dioxide / Q000097:blood*; D002432:Cecum / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D004032:Diet; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism* / Q000382:microbiology; D007700:Kinetics; D008297:Male; D051379:Mice; D011180:Postmortem Changes; D051381:Rats; D013045:Species Specificity
chemical_list: D002245:Carbon Dioxide