Effect of tetrandrine on neutrophilic recruitment response to brain ischemia/reperfusion.
OBJECTIVE
To investigate the effect of tetrandrine (Tet) on neutrophilic recruitment response to bain ischemia/reperfusion (I/R).


METHODS
Middle cerebral artery (MCA) ischemia (2 h)/reperfusion model was built on rats (male). Brain water content, neutrophilic recruitment, the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and activation of NF-kappaB in cellular nucleus after brain I/R were measured with dry-wet weight, 51Cr-labeled neutrophil, reverse transcriptase polymerase chain reaction, and electrophoretic mobility shift assay, respectively.


RESULTS
Brain water and neutrophilic recruitment were parallelly increased from 3 h to 24 h after reperfusion (P < 0.01). The expression of ICAM-1 mRNA was detected at 1 h after reperfusion (P < 0.01), increased to the highest peak at 12 h (P < 0.01), and at 24 h decreased to level at 3 h. The activation of NF-kappaB was detected at 0.5 h after reperfusion (P < 0.01), increased to the highest peak at 6 h (P < 0.01), and at 24 h decreased to level at 1 h. Tet 10 and 20 mg/kg decreased brain water content, neutrophilic recruitment, the expression of ICAM-1 mRNA, and the activation of NF-kappaB at 6 h, 12 h, and 24 h after reperfusion.


CONCLUSIONS
Tet inhibited neutrophilic recruitment, expression of ICAM-1 mRNA, and activation of NF-kappaB after brain I/R.
Liu
S J
SJ
Department of Clinical Pharmacology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. liu.shi-jie@163.net
Zhou
S W
SW
Xue
C S
CS
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D001921:Brain / Q000473:pathology; D001929:Brain Edema / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology*
D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; C009438:tetrandrine