Amino acid transport by intestinal brush border vesicles of a marine fish, Boops salpa.
The transport of glycine, alanine, methionine and alpha amino-isobutyric acid (AIB) was studied on brush border membrane vesicles of Boops salpa, a marine fish. This transport was Na(+)-, Cl(-)- and pH-dependent. In the presence of NaCl, the uptake decreased as the pH increased from 5.5 to 8.5. With Na2SO4, the transport of the four amino acids was strongly reduced and the pH optimum was 7-8. In the presence of NaCl, amino acid transport was described by high and low affinity kinetics. The K(t) of the high-affinity component was comparable for glycine, alanine and methionine (0.1 mM), and was significantly enhanced for AIB (0.6 mM). The J(max) of the low affinity component was significantly lower for methionine and AIB than for glycine and alanine. Lowering the sodium concentration from 80 to 20 mM significantly increased K(t) and J(max) of the high-affinity component of glycine transport. Moreover, the kinetics of AIB transport under 100 mM Na(+) were similar to glycine kinetics under 40 mM Na(+) and the two amino acids competed for the same carrier(s). These results suggest that chloride ions are essential in neutral amino acid transport in Boops, that multiple saturable components are involved in this process, and that sodium plays an important role in the differences between the transport kinetics of amino acids.
Bogé
Gérard
G
Université de Toulon, LEPI and Département de Génie Biologique, BP 132, F-83957 Cedex, La Garde, France. boge@univ-tln.fr
Roche
Hélène
H
Balocco
Catherine
C
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D005399:Fishes / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption; D007700:Kinetics; D008522:Mediterranean Sea; D008715:Methionine / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D012965:Sodium Chloride; D012964:Sodium; D008715:Methionine; D000409:Alanine; D005998:Glycine