title: Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae.
abstract: To elucidate the roles of genes involved in the cell wall biogenesis and function in Saccharomyces cerevisiae, we isolated and characterized mutants that were lethal in a strain in which the SED1 gene encoding a cell wall mannoprotein was disrupted. Thus, double mutants of SED1 and either MNN9 or MNN10 were unable to grow and YOL155c on a multicopy plasmid could suppress their synthetic lethality. A Yol155cp-GFP fusion protein was found to localize to the cell wall, suggesting that it might also be a cell wall mannoprotein. Subsequently, we analysed the effects of the shut-off of SED1 in a sed1 and mnn9 double mutant: cells after the shut-off showed anomalous cellular morphology and died in the mitotic M phase. From these and other results, we postulate that these genes function cooperatively with each other and in a cell cycle-dependent manner in the biogenesis and maintenance of cell wall in S. cerevisiae.
authors.affiliation: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokkodai, Kobe 657-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Horie
authors.forename: K
authors.initials: K
authors.lastname: Isono
mesh_terms: D000595:Amino Acid Sequence; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002473:Cell Wall / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D008364:Mannosyltransferases / Q000235:genetics / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; C086605:MNN9 protein, S cerevisiae; D008562:Membrane Glycoproteins; C077394:SED1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins; D008364:Mannosyltransferases; C117964:Mnn10 protein, S cerevisiae