Isolation and quantification of the heat shock protein 90 alpha and beta isoforms from rat liver.
Heat shock protein 90 (Hsp90) is an abundant cytosolic protein. In higher eukaryotes two isoforms of Hsp90 exist, Hsp90 alpha and Hsp90 beta. Hsp90 was purified from rat liver and after sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a double band at about 90 kDa. The two bands were separated and identified as the Hsp90 alpha and Hsp90 beta isoforms. There was no entry in the protein databases for the Hsp90 alpha isoform from rat. Furthermore, the ratio of the two Hsp90 isoforms was determined.
Langer
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Institut für Biochemie, Johann-Wolfgang-Goethe-Universität Frankfurt am Main, Marie-Curie-Strasse 9, 60439 Frankfurt am Main, Federal Republic of Germany.
Fasold
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H
D000595:Amino Acid Sequence; D000818:Animals; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020033:Protein Isoforms; D051381:Rats; D017207:Rats, Sprague-Dawley; D016415:Sequence Alignment
D018841:HSP90 Heat-Shock Proteins; D020033:Protein Isoforms