title: CD8 binding to MHC class I molecules is influenced by T cell maturation and glycosylation.
abstract: CD8 serves both as an adhesion molecule for class I MHC molecules and as a coreceptor with the TCR for T cell activation. Here we study the developmental regulation of CD8-mediated binding to noncognate peptide/MHC ligands (i.e., those not bound by the TCR). We show that CD8's ability to bind soluble class I MHC tetramers and to mediate T cell adhesion under shear flow conditions diminishes as double-positive thymocytes mature into CD8(+) T cells. Furthermore, we provide evidence that this decreased CD8 binding results from increased T cell sialylation upon T cell maturation. These data suggest that CD8's ability to interact with class I MHC is not fixed and is developmentally regulated through the T cell's glycosylation state.
authors.affiliation: Center for Immunology, Department of Lab Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Daniels
authors.forename: L
authors.initials: L
authors.lastname: Devine
authors.forename: J D
authors.initials: JD
authors.lastname: Miller
authors.forename: J M
authors.initials: JM
authors.lastname: Moser
authors.forename: A E
authors.initials: AE
authors.lastname: Lukacher
authors.forename: J D
authors.initials: JD
authors.lastname: Altman
authors.forename: P
authors.initials: P
authors.lastname: Kavathas
authors.forename: K A
authors.initials: KA
authors.lastname: Hogquist
authors.forename: S C
authors.initials: SC
authors.lastname: Jameson
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000818:Animals; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000276:immunology / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D016922:Cellular Senescence; D006031:Glycosylation; D006183:H-2 Antigens / Q000276:immunology / Q000378:metabolism*; D064532:Histocompatibility Antigen H-2D; D008024:Ligands; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000172:deficiency / Q000235:genetics; D012212:Rheology; D012995:Solubility; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D017252:CD3 Complex; D016827:CD8 Antigens; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D; D008024:Ligands; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta; C073119:TAP1 protein, human; C486171:Tap1 protein, mouse; D010047:Ovalbumin; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid