title: Antioxidant supplementation and exercise-induced oxidative stress in the 60-year-old as measured by antipyrine hydroxylates.
abstract: The effects of 12 weeks of antioxidant supplementation on exercise-induced oxidative stress were investigated in older adults (60 (SE 1) years; BMI 26 (SE 1) kg/m(2)). Subjects were randomly divided in two groups: supplementation (n 11) with 100 mg dl-alpha-tocopheryl acetate, 200 mg ascorbic acid, and 2 mg beta-carotene, and placebo (n 9). Before and after the 12 week supplementation period, subjects cycled for 45 min at submaximal intensity (50 % maximal workload capacity). Antipyrine was used as marker for oxidative stress. Antipyrine reacts quickly with hydroxyl radicals to form para- and ortho-hydroxyantipyrine. The latter metabolite is not formed in man through the mono-oxygenase pathway of cytochrome P450. Daily supplementation significantly increased plasma concentrations of alpha-tocopherol and beta-carotene in the supplemented group (Delta 14.4 (SE 3.2) and 0.4 (se 0.1) micromol/l; P<0.001 and P<0.01). No significant differences, within and between groups, were observed in the exercise-induced increase in the ratios para- and ortho-hydroxyantipyrine to antipyrine. In addition, supplementation did not affect the exercise-induced increase in thiobarbituric acid reactive substances in plasma. In conclusion, in 60-year-old subjects antioxidant supplementation had no effect on the exercise-induced increase in oxidative stress as measured by free radical products of antipyrine.
authors.affiliation: Department of Human Biology, Maastricht University, Maastricht, The Netherlands. e.meijer@hb.unimaas.nl
authors.forename: E P
authors.initials: EP
authors.lastname: Meijer
authors.forename: A H
authors.initials: AH
authors.lastname: Goris
authors.forename: J
authors.initials: J
authors.lastname: Senden
authors.forename: J L
authors.initials: JL
authors.lastname: van Dongen
authors.forename: A
authors.initials: A
authors.lastname: Bast
authors.forename: K R
authors.initials: KR
authors.lastname: Westerterp
mesh_terms: D000975:Antioxidants / Q000008:administration & dosage*; D000983:Antipyrine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004311:Double-Blind Method; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D006900:Hydroxylation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D000983:Antipyrine