Protective effect of T-type calcium channel blocker in histamine-induced paw inflammation in rat.
The aim of this paper was to investigate the protective effect of the T-type calcium channel blocker in a model of acute local inflammation (histamine-induced). The intraplantar injection of histamine elicited an inflammatory response that was characterized by a time-dependent increase in paw oedema and neutrophil infiltration in paw tissue. The maximal increase in paw volume was observed at 90 min after histamine administration (maximal paw volume: 0.97 +/- 0.07). In addition, polymorphonuclear leucocyte (PNL) number was markedly increased in the histamine-treated paw tissue (144 +/- 25.56). However, histamine-induced paw oedema was significantly reduced in a dose-dependent manner by treatment with mibefradil (given at 10, 25, 50, and 100 mg x kg(-1)) at 30, 60, 90, 120, 150, 180 min after injection of histamine. Mibefradil treatment also caused a significant reduction of the polymorphonuclear leucocyte number in the paw tissue. Our findings support the view that mibefradil exerts antiinflammatory effects.
Bilici
D
D
Department of Pharmacology, Medical School, Atatürk University, Turkey. dilek-bilici@yahoo.com
Akpinar
E
E
Gürsan
N
N
Dengiz
G O
GO
Bilici
S
S
Altaş
S
S
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020747:Calcium Channels, T-Type / Q000187:drug effects*; D002634:Chemotaxis, Leukocyte; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D006632:Histamine; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D020748:Mibefradil / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D020748:Mibefradil; D006632:Histamine; D007213:Indomethacin