title: Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor.
abstract: A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism.
authors.affiliation: The Children's Hospital of Philadelphia, Division of Immunologic and Infectious Diseases, Abramson 1202, 3516 Civic Center Boulevard, Philadelphia, PA 19104, USA1.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Cohen
authors.affiliation: The Wistar Institute, Philadelphia, PA 19104, USA2.
authors.forename: Zhi Quan
authors.initials: ZQ
authors.lastname: Xiang
authors.affiliation: The Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA3.
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Gao
authors.affiliation: The Wistar Institute, Philadelphia, PA 19104, USA2.
authors.forename: Hildegund C J
authors.initials: HCJ
authors.lastname: Ertl
authors.affiliation: The Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA3.|The Wistar Institute, Philadelphia, PA 19104, USA2.
authors.forename: James M
authors.initials: JM
authors.lastname: Wilson
authors.affiliation: The Children's Hospital of Philadelphia, Division of Immunologic and Infectious Diseases, Abramson 1202, 3516 Civic Center Boulevard, Philadelphia, PA 19104, USA1.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Bergelson
mesh_terms: D000222:Adaptation, Physiological; D000260:Adenoviruses, Human / Q000378:metabolism*; D000261:Adenoviruses, Simian / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D004770:Enterovirus / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014161:Transduction, Genetic
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D008164:Luminescent Proteins; D011991:Receptors, Virus; D049452:Green Fluorescent Proteins