title: Isolation of X and Y chromosome-specific DNA markers from a liverwort, Marchantia polymorpha, by representational difference analysis.
abstract: The liverwort Marchantia polymorpha has X and Y chromosomes in the respective female and male haploids. Here we report the successful exploitation of representational difference analyses to isolate DNA markers for the sex chromosomes. Two female-specific and six male-specific DNA fragments were genetically confirmed to originate from the X and Y chromosomes, respectively.
authors.affiliation: Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujisawa
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Nishio
authors.forename: T
authors.initials: T
authors.lastname: Bando
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: K T
authors.initials: KT
authors.lastname: Yamato
authors.forename: H
authors.initials: H
authors.lastname: Fukuzawa
authors.forename: K
authors.initials: K
authors.lastname: Ohyama
mesh_terms: D002875:Chromosomes / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D005819:Genetic Markers