[In vitro effect of rainbow trout (Oncorhynchus mykiss) serum in Tylodelphys sp. (trematoda, Diplosromatidae) metacercariae].
Here, it has been described that normal fresh sera from rainbow trout (Oncorhynchus mykiss) were able to lyse Tylodelphys sp. metacercariae isolated from Galaxias maculatus (Jenyns, 1842) (Teleostei, Galaxiidae) brain. This effect was time dependent, and progressively increased since 1 h up to 18 h incubation. There were oscillations in the ability of different sera samples to lyse these parasites. Similar values were achieved with other salmonid sera, and also with normal human sera pool. The lytic activity was abolished by heat-inactivated sera, by zymosan treatment and by incubation in the presence of EDTA. However, the mean values obtained in the presence of EGTA-Mg++ did not significantly differ from those found with sera as a source of complement. The results of this study suggested the role of complement alternative pathway on Tylodephys sp. in vitro lysis, under our experimental conditions.
Olabuenaga
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Centro Regional Universitario Bariloche, Unidad Postal Universidad Nacional del Comahue, 8400 Bariloche, Argentina.
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000097:blood* / Q000276:immunology; D013997:Time Factors; D014200:Trematoda / Q000276:immunology*; D015054:Zymosan / Q000494:pharmacology
D004533:Egtazic Acid; D003165:Complement System Proteins; D015054:Zymosan; D004492:Edetic Acid