title: Neurotrophin receptor expression and responsiveness by postnatal cerebral oligodendroglia.
abstract: The low affinity neurotrophin receptor (p75(NTR)) is implicated in promoting oligodendrocytic death after nerve growth factor (NGF) stimulation but NGF and neurotrophin-3 (NT-3) can also potentiate oligodendrocytic survival. We show regional variability in p75(NTR) expression within the central nervous system of the postnatal rat; expression is readily detectable by immunohistochemistry upon a subset of CNPase-positive oligodendroglia in optic nerve but not within the cerebrum. Nevertheless, oligodendroglia isolated from the cerebrum and cultured for 16 hours express p75(NTR) as well as the trkC but not the TrkA gene. Viability was not, however, influenced by exposure to either NGF or NT-3. Cells overexpressing p75(NTR) remained unresponsive to NGF but exhibited potentiated survival with NT-3, correlating with the differential expression profile of their high affinity receptors.
authors.affiliation: Development and Neurobiology Group, The Walter and Eliza Hall Institute of Medical Research, Post Office, The Royal Melbourne Hospital Parkville 3050 Victoria, Australia.
authors.forename: G D
authors.initials: GD
authors.lastname: Starkey
authors.forename: S
authors.initials: S
authors.lastname: Petratos
authors.forename: K A
authors.initials: KA
authors.lastname: Shipham
authors.forename: H
authors.initials: H
authors.lastname: Butzkueven
authors.forename: T
authors.initials: T
authors.lastname: Bucci
authors.forename: K
authors.initials: K
authors.lastname: Lowry
authors.forename: S S
authors.initials: SS
authors.lastname: Cheema
authors.forename: T J
authors.initials: TJ
authors.lastname: Kilpatrick
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D005822:Genetic Vectors; D007150:Immunohistochemistry; D007763:Lac Operon; D009836:Oligodendroglia / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020800:Receptor, Nerve Growth Factor / Q000032:analysis / Q000235:genetics; D020917:Receptor, trkA / Q000235:genetics; D020812:Receptor, trkC / Q000032:analysis / Q000235:genetics; D017475:Receptors, Nerve Growth Factor / Q000032:analysis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D020917:Receptor, trkA; D020812:Receptor, trkC