title: In vitro antimicrobial activity of a chitooligosaccharide mixture against Actinobacillus actinomycetemcomitans and Streptococcus mutans.
abstract: The purpose of this study was to evaluate the in vitro antibacterial activity of a chitooligosaccharide mixture (MW 2000-30000 Da) with a deacetylation degree of 91.5% against two representative oral pathogens, Actinobacillus actinomycetemcomitans and Streptococcus mutans. A 0.1% concentration of the chitooligosaccharides (derived from the exoskeletons of marine crustaceans) was used to estimate antibacterial activity. Approximately 2 logcolony forming units (CFU)/ml of A. actinomycetemcomitans were inactivated by 0.1% chitosan after 30 min, while 120 min exposure inactivated about 4.5 logCFU/ml of this organism. In contrast, the level of inactivation against S. mutans was less than 0.5 logCFU/ml after an exposure of up to 120 min. Electron microscopy showed that the exposure of A. actinomycetemcomitans to the chitooligosaccharides resulted in the disruption of cell membranes and that it could be considered for the treatment of periodontal diseases associated with A. actinomycetemcomitans.
authors.affiliation: Department of Oral Biology, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodaemun-Gu, Seoul, South Korea.
authors.forename: B K
authors.initials: BK
authors.lastname: Choi
authors.forename: K Y
authors.initials: KY
authors.lastname: Kim
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yoo
authors.forename: S J
authors.initials: SJ
authors.lastname: Oh
authors.forename: J H
authors.initials: JH
authors.lastname: Choi
authors.forename: C Y
authors.initials: CY
authors.lastname: Kim
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001704:Biopolymers / Q000494:pharmacology; D002686:Chitin / Q000031:analogs & derivatives / Q000494:pharmacology*; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D013295:Streptococcus mutans / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001704:Biopolymers; D002686:Chitin; D048271:Chitosan