title: Classical MHC class I genes composed of highly divergent sequence lineages share a single locus in rainbow trout (Oncorhynchus mykiss).
abstract: The classical MHC class I genes have been known to be highly polymorphic in various vertebrates. To date, putative allelic sequences of the classical MHC class I genes in teleost fish have been reported in several studies. However, the establishment of their allelic status has been hampered in most cases by the lack of appropriate genomic information. In the present study, using heterozygous and homozygous fish, we obtained classical-type MHC class I sequences of rainbow trout (Oncorhynchus mykiss) and investigated their allelic relationship by gene amplification and Southern and Northern hybridization analyses. The results indicated that all MHC class I sequences we obtained were derived from a single locus. Based on this, a unique polymorphic nature of the MHC class I locus of rainbow trout has been revealed. The mosaic combination of highly divergent ancient sequences in the peptide-binding domains is notable, and the variable nature around the boundary between the alpha3 and transmembrane domains is unprecedented.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie 519-0423, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Dijkstra
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
authors.forename: Ikuo
authors.initials: I
authors.lastname: Denda
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ototake
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D019143:Evolution, Molecular; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D012474:Salmon / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger