Parathion induces mouse germ cells apoptosis.
Germ cell loss occurs in normal spermatogenesis at defined stages of the seminiferous epithelial cycle. The process has been known for over a century but only recently it was analyzed under the concept of apoptosis. This is a programmed cell death that occurs during development and also in the adult. It is believed to play a key role as quality control in sperm formation, avoiding the passage of genetic defects to future generations. Chemical toxicants may increase apoptosis, disturbing tissue homeostasis. The effect of the agropesticide parathion upon apoptosis in mouse seminiferous tubules was analyzed in young mice (onset of spermatogenesis) and in adult animals (full spermatogenesis). In both young and adult mice, the pesticide increases the rate of apoptosis, which takes place at stages where spermatogonial proliferation occurs, affects spermatocytes at the beginning of the meiotic process and spermatids at the elongating period. Basal apoptotic rates are greater in young mice. In adults, commercial parathion is more toxic than the pure organophosphoric compound. From these observations plus in vitro effects of parathion reported previously, it can be concluded that the pesticide affects DNA (and RNA and protein) synthesis. The effect is reversible with moderate doses of the chemical after acute intoxication.
Bustos-Obregón
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Laboratory of Biology of Reproduction, Program of Morphology, University of Chile Medical School Santiago. ebustos@machi.med.uchile.cl
Díaz
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Sobarzo
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D000375:Aging; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D010278:Parathion / Q000494:pharmacology*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*
D007306:Insecticides; D010278:Parathion