Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae.
To elucidate the roles of genes involved in the cell wall biogenesis and function in Saccharomyces cerevisiae, we isolated and characterized mutants that were lethal in a strain in which the SED1 gene encoding a cell wall mannoprotein was disrupted. Thus, double mutants of SED1 and either MNN9 or MNN10 were unable to grow and YOL155c on a multicopy plasmid could suppress their synthetic lethality. A Yol155cp-GFP fusion protein was found to localize to the cell wall, suggesting that it might also be a cell wall mannoprotein. Subsequently, we analysed the effects of the shut-off of SED1 in a sed1 and mnn9 double mutant: cells after the shut-off showed anomalous cellular morphology and died in the mitotic M phase. From these and other results, we postulate that these genes function cooperatively with each other and in a cell cycle-dependent manner in the biogenesis and maintenance of cell wall in S. cerevisiae.
Horie
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Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokkodai, Kobe 657-8501, Japan.
Isono
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D000595:Amino Acid Sequence; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002473:Cell Wall / Q000235:genetics / Q000502:physiology / Q000648:ultrastructure; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000235:genetics* / Q000502:physiology; D008364:Mannosyltransferases / Q000235:genetics / Q000502:physiology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; C086605:MNN9 protein, S cerevisiae; D008562:Membrane Glycoproteins; C077394:SED1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins; D008364:Mannosyltransferases; C117964:Mnn10 protein, S cerevisiae