Stability of bisphenol A, triethylene-glycol dimethacrylate, and bisphenol A dimethacrylate in whole saliva.
OBJECTIVE
This study investigated the stability of compounds of dental sealant materials in a salivary matrix.


METHODS
Various amounts of bisphenol A (BPA), bisphenol A dimethacrylate (BIS-DMA) or triethylene-glycol dimethacrylate (TEGDMA) were added to whole salivary samples, and stored at -70 degrees C or -20 degrees C for up to 4 months. In other experiments, four separate whole salivary or water samples with BIS-DMA (200 ng/ml) were incubated for 0, 1, 2, 4 or 24h at 37 degrees C. Levels of analytes were determined by capillary gas chromatography/mass spectrophotometry (GC/MS) and high-performance liquid chromatography (HPLC).


RESULTS
BPA was stable under all tested conditions. Samples originally containing BIS-DMA had high levels of BPA and almost no BIS-DMA after 4 months at -20 degrees C. Salivary samples incubated at 37 degrees C originally containing only BIS-DMA (200 ng/ml) demonstrated rapid decreases of BIS-DMA and increases of BPA. By 24h, the mean BIS-DMA concentration fell to 21.8 (25) ng/ml, while BPA increased to 100 (48) ng/ml. Only slight decreases in BIS-DMA and no BPA were present in the water samples incubated at 37 degrees C. BPA, BIS-DMA, and TEGDMA were stable if salivary samples were stored at -70 degrees C. Acidification of salivary samples prevented the breakdown of BIS-DMA.


CONCLUSIONS
BIS-DMA is converted rapidly to BPA in the presence of whole saliva. This could account for the findings of BPA in clinical samples collected after the placement of certain sealant products. Decreasing salivary pH and temperature can slow this process and this method should be used for clinical studies of salivary BPA leached from restorative materials.
Atkinson
Jane C
JC
Clinical Research Core, National Institute of Dental and Craniofacial Research/NIH, Bethesda, MD, USA. jatkinson@dental.umaryland.edu
Diamond
Francis
F
Eichmiller
Frederick
F
Selwitz
Robert
R
Jones
Gordon
G
D000143:Acids / Q000737:chemistry; D000681:Amylases / Q000032:analysis; D000704:Analysis of Variance; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D003188:Composite Resins / Q000032:analysis / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008689:Methacrylates / Q000032:analysis / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry*; D010895:Pit and Fissure Sealants / Q000032:analysis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000032:analysis / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology; D013223:Statistics as Topic; D013696:Temperature; D013997:Time Factors; D000098826:Bisphenol A Compounds
D000143:Acids; D000681:Amylases; D001559:Benzhydryl Compounds; D003188:Composite Resins; D008689:Methacrylates; D010636:Phenols; D010895:Pit and Fissure Sealants; D011092:Polyethylene Glycols; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; C002462:4,4'-isopropylidenediphenol dimethacrylate; D000098826:Bisphenol A Compounds; C006780:bisphenol A