Light and electron microscopic study of corticorubral synapses in adult cat: evidence for extensive synaptic remodeling during postnatal development.
Spine-like dendritic protrusions (SLDPs) emanating from developing dendrites have been proposed to play an important role in early synaptogenesis. We previously analyzed synaptic termination sites on soma-dendritic membrane of newborn cats and found that corticorubral (CR) axons form synapses preferentially on SLDPs (Saito et al., 1997). In the present study, we examined CR synapses in adult cats to elucidate the maturation process of CR synapses in relation to SLDPs. Electron microscopic observation of serial thin sections of Phaseolus vulgaris-leucoagglutinin-labeled axons revealed that approximately 60% of CR terminals in adult cats formed synapses on dendritic spines. We also found that CR axons terminate on dendritic spines originating from the intermediate or distal dendrites of rubrospinal cells (more than 200 microm apart from the soma), in contrast to kittens in which CR fibers terminate on SLDPs originating from the proximal dendrites (less than 100 microm apart from the soma) of rubrospinal cells (Saito et al. [1997] J. Neurosci. 17:8792-8803). These results suggest that CR synapses undergo remarkable remodeling after initial termination on SLDP during postnatal development.
Saito
Y
Y
Laboratory of Neuroscience, Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
Katsumaru
H
H
Wilson
C J
CJ
Murakami
F
F
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology / Q000254:growth & development; D002415:Cats / Q000033:anatomy & histology* / Q000502:physiology; D002540:Cerebral Cortex / Q000502:physiology / Q000648:ultrastructure*; D003712:Dendrites / Q000648:ultrastructure; D008854:Microscopy, Electron; D009473:Neuronal Plasticity; D012012:Red Nucleus / Q000502:physiology / Q000648:ultrastructure*; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*