title: Expression of IL-17B in neurons and evaluation of its possible role in the chromosome 5q-linked form of Charcot-Marie-Tooth disease.
abstract: IL-17B is a recently identified homolog of IL-17. Northern analysis revealed that IL-17B mRNA is expressed at very high levels in spinal cord and at much lower and more variable levels in trachea, prostate, lung, small intestine, testes, adrenal, and pancreas. In developing mouse embryos IL-17B expression was first detected at day 11 and appeared to peak at day 15. In situ analysis of mouse spinal cord, dorsal root ganglia, and brain demonstrated that IL-17B mRNA is primarily expressed by the neurons. Immunohistochemical analysis of human spinal cord, dorsal root ganglia, cerebral cortex, cerebellum, and hippocampus demonstrated that IL-17B protein is primarily localized to the neuronal cell bodies and axons. Radiation hybrid mapping localized the IL-17B gene to a region on human chromosome 5q that is associated with a rare autosomal recessive form of Charcot-Marie-Tooth demyelinating disease. However, no changes were found in the coding regions, splice junctions, intron 1, or the 5' and 3' untranslated regions of IL-17B genes of patients affected with this disease.
authors.affiliation: ZymoGenetics Inc, 1201 Eastlake Avenue East, Seattle, WA 98102, USA. mooreb@zgi.com
authors.forename: Emma E
authors.initials: EE
authors.lastname: Moore
authors.forename: Scott
authors.initials: S
authors.lastname: Presnell
authors.forename: Ursula
authors.initials: U
authors.lastname: Garrigues
authors.forename: Angele
authors.initials: A
authors.lastname: Guilbot
authors.forename: Eric
authors.initials: E
authors.lastname: LeGuern
authors.forename: Deborah
authors.initials: D
authors.lastname: Smith
authors.forename: Lena
authors.initials: L
authors.lastname: Yao
authors.forename: Theodore E
authors.initials: TE
authors.lastname: Whitmore
authors.forename: Teresa
authors.initials: T
authors.lastname: Gilbert
authors.forename: Theo D
authors.initials: TD
authors.lastname: Palmer
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Horner
authors.forename: Rolf E
authors.initials: RE
authors.lastname: Kuestner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000276:immunology; D002460:Cell Line; D002607:Charcot-Marie-Tooth Disease / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D006224:Cricetinae; D005314:Embryonic and Fetal Development; D020224:Expressed Sequence Tags; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015723:Gene Library; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009474:Neurons / Q000276:immunology* / Q000502:physiology*; D009928:Organ Specificity; D011467:Prostate / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000276:immunology; D014132:Trachea / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D020381:Interleukin-17; D012333:RNA, Messenger