Highly polymerized procyanidins in brown soybean seed coat with a high radical-scavenging activity.
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extract from the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of the seed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, but lower than that of two black varieties, and was closely dependent on the content of phenolic compounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavenging activities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis showed that the degree of polymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was as high as DP30.
Takahata
Y
Y
National Agricultural Research Center for Kyushu Okinawa Region, National Agricultural Research Organization (KONARC/NARO), Nishigoshi, Kumamoto 861-1192, Japan.
Ohnishi-Kameyama
M
M
Furuta
S
S
Takahashi
M
M
Suda
I
I
D000872:Anthocyanins / Q000032:analysis; D044946:Biflavonoids; D002392:Catechin / Q000032:analysis; D019281:Dimerization; D016166:Free Radical Scavengers / Q000032:analysis*; D044945:Proanthocyanidins; D013025:Glycine max / Q000737:chemistry*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000872:Anthocyanins; D044946:Biflavonoids; D016166:Free Radical Scavengers; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin