title: RANTES release by human airway smooth muscle: effects of prostaglandin E(2) and fenoterol.
abstract: In human airway smooth muscle cells, the levels of RANTES were increased upon stimulation with interleukin-1beta together with tumour necrosis factor-alpha (TNF-alpha) (10 ng ml(-1) for each). In this study, we have assessed the effects of prostaglandin E(2) and the beta(2)-adrenoceptor agonist, fenoterol on RANTES (regulated upon activation, normal T cell expressed and secreted) release by these cells. The levels of RANTES released by human airway smooth muscle cells were measured after 24 h of treatment. Prostaglandin E(2) and fenoterol, only in presence of a cyclo-oxygenase inhibitor indomethacin (10(-6) M), provoked a concentration-dependent reduction in RANTES release. These data suggest that, in settings where cyclo-oxygenase activity is low, both drugs may relieve the symptoms of airway diseases by reducing RANTES production.
authors.affiliation: Thoracic Medicine, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK.
authors.forename: N
authors.initials: N
authors.lastname: Lazzeri
authors.forename: M G
authors.initials: MG
authors.lastname: Belvisi
authors.forename: H J
authors.initials: HJ
authors.lastname: Patel
authors.forename: K F
authors.initials: KF
authors.lastname: Chung
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
authors.forename: J A
authors.initials: JA
authors.lastname: Mitchell
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000328:Adult; D018946:Chemokine CCL5 / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005280:Fenoterol / Q000494:pharmacology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D014132:Trachea / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000318:Adrenergic beta-Agonists; D018946:Chemokine CCL5; D005280:Fenoterol; D000242:Cyclic AMP; D015232:Dinoprostone; D007213:Indomethacin