Cellular immune response against tropomyosin isoform 5 in ulcerative colitis.
We have reported an autoantibody response in ulcerative colitis (UC) against human tropomyosin isoform 5 (hTM5), the predominant colonic epithelial cell hTM isoform. In this report, we determined the number of IFN-gamma-secreting cells (spot-forming cells, SFC) against hTM5 by an enzyme-linked immunospot (ELISPOT) assay. Another cytoskeletal protein, caldesmon, CaD40, was used as a control antigen. Peripheral blood mononuclear cells were separated by a Ficoll density gradient from 28 patients with UC, 13 patients with Crohn's disease (CD), and 9 healthy subjects (HS). The mean (+/-SEM) SFC values against hTM5 in UC, CD, and HS were 48.8 +/- 8.1, 18.6 +/- 4.6, and 20.8 +/- 8.6, respectively. The value in UC was significantly higher than those in CD (P < 0.005) and HS (P < 0.025). SFC values in CD did not differ from those in HS. None of the 50 samples (except 1 UC) reacted to the CaD40 antigen. This study demonstrates, for the first time, a defined colon epithelial cell antigen, hTM5, that is capable of inducing a significant T cell response in UC but not in CD.
Taniguchi
M
M
Crohn's and Colitis Center of New Jersey, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA.
Geng
X
X
Glazier
K D
KD
Dasgupta
A
A
Lin
J J
JJ
Das
K M
KM
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D002148:Calmodulin-Binding Proteins / Q000276:immunology; D003093:Colitis, Ulcerative / Q000276:immunology*; D003424:Crohn Disease / Q000276:immunology; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000276:immunology; D008297:Male; D008875:Middle Aged; D014335:Tropomyosin / Q000276:immunology*
D001323:Autoantibodies; D002148:Calmodulin-Binding Proteins; C498783:TPM3 protein, human; D014335:Tropomyosin; D007371:Interferon-gamma