title: C-MYC and IGF-II mRNA-binding protein (CRD-BP/IMP-1) in benign and malignant mesenchymal tumors.
abstract: Mouse coding region determinant-binding (mCRD-BP) and human IGF-II mRNA-binding 1 (hIMP-1) proteins are orthologous mRNA-binding proteins that recognize c-myc and IGF-II mRNA, respectively, and regulate their expression posttranscriptionally. Here, we confirm that human CRD-BP/IMP-1 binds to c-myc mRNA and that it is predominantly expressed in fetal tissues. Moreover, hCRD-BP/IMP-1 expression was detected in cell lines of neoplastic origin and in selected primary tumors. In a series of 33 malignant and 10 benign mesenchymal tumors, 73% and 40%, respectively, were found to express hCRD-BP/IMP-1. In particular, expression was significant in 14 Ewing's sarcomas, all of which were positive. The data suggest that hCRD-BP/IMP-1 plays a role in abnormal cell proliferation in mesenchymal tumors.
authors.affiliation: Department of Genetics, St. Savas Hospital, Athens, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Ioannidis
authors.forename: T
authors.initials: T
authors.lastname: Trangas
authors.forename: E
authors.initials: E
authors.lastname: Dimitriadis
authors.forename: M
authors.initials: M
authors.lastname: Samiotaki
authors.forename: I
authors.initials: I
authors.lastname: Kyriazoglou
authors.forename: C M
authors.initials: CM
authors.lastname: Tsiapalis
authors.forename: C
authors.initials: C
authors.lastname: Kittas
authors.forename: N
authors.initials: N
authors.lastname: Agnantis
authors.forename: F C
authors.initials: FC
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: J
authors.initials: J
authors.lastname: Christiansen
authors.forename: N
authors.initials: N
authors.lastname: Pandis
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008637:Mesenchymoma / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000378:metabolism; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; C406144:IGF2BP1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012313:RNA