High intralesional interleukin-10 messenger RNA expression in localized cutaneous leishmaniasis is associated with unresponsiveness to treatment.
The intralesional expression of cytokines (interleukin [IL]-4, IL-13, IL-10, and interferon-gamma) was analyzed in 65 patients with localized cutaneous leishmaniasis due to Leishmania guyanensis before specific treatment with pentamidine isethionate. The local expression of IL-10 was significantly higher in patients who responded poorly to treatment than in patients whose lesions were regressing. When an IL-10 level >10 (ratio of the concentration of IL-10 [pg/microL] to that of beta-actin [pg/microL]) was used as an indicator of treatment failure, the sensitivity of this test was 78.6, and the specificity was 72.5. Thus, high intralesional expression of IL-10 might predict a poor response to conventional treatment.
Bourreau
E
E
Immunologie des Leishmanioses, Institute Pasteur, Cayenne, French Guiana. plaunois@pasteur-cayenne.fr
Prévot
G
G
Gardon
J
J
Pradinaud
R
R
Launois
P
P
D000293:Adolescent; D000328:Adult; D000818:Animals; D000981:Antiprotozoal Agents / Q000627:therapeutic use*; D002648:Child; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D018334:Leishmania guyanensis / Q000187:drug effects*; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010419:Pentamidine / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D017211:Treatment Failure
D000981:Antiprotozoal Agents; D016207:Cytokines; D012333:RNA, Messenger; D016753:Interleukin-10; D010419:Pentamidine