title: Glucocorticoid induces apoptosis in rat leydig cells.
abstract: The aim of the present study was to investigate whether glucocorticoid induces apoptosis in rat Leydig cells. To determine whether there are developmental differences in glucocorticoid sensitivity, Leydig cells were isolated at distinct stages of their differentiation [mesenchymal-like progenitors (PLC), immature Leydig cells (ILC), and adult Leydig cells (ALC)] from 21-, 35-, and 90-d-old Sprague Dawley rats, respectively. Glucocorticoid induction of apoptosis was evaluated after both in vitro and in vivo exposures. In the first set of experiments, PLC, ILC, and ALC were treated with 100 nM corticosterone (CORT) for either 4 or 24 h in vitro and then assessed for labeling with the apoptotic marker annexin V. PLC exposed to CORT had levels of annexin V-fluorescein isothiocyanate labeling that were unchanged relative to control values at both time points (P > 0.05). In contrast, CORT-treated ILC and ALC had increased frequencies of apoptosis: in ALC, a 22.1 +/- 1.7% incidence after 4 h and 30.5 +/- 2.3% after 24 h compared with 7.4 +/- 0.8% in untreated controls (P < 0.05). Similar trends were observed for ILC. Ultrastructural analysis confirmed that the increase in annexin V labeling was associated with characteristic signs of apoptosis, including nuclear fragmentation and formation of apoptotic bodies. A second line of experiments examined whether apoptosis was evident in purified Leydig cells after administration of CORT in vivo. Male rats were subjected to bilateral adrenalectomy and were treated with CORT by ip injection twice daily at doses ranging from 2.5-7.5 mg/100 g BW starting 3 d after surgery. The frequency of Leydig cell apoptosis was measured at 12, 24, 48, and 72 h after the first injection. Administration of the 2.5-mg dose raised circulating CORT 5-10 times above normal basal concentrations, and LH levels sampled at these times were not altered in the treated animals. Increased Leydig cell apoptosis was measurable after 24 h of treatment, with an incidence of 21.1 +/- 1.8% in ALC compared with 5.7 +/- 0.8% in untreated controls (P < 0.05). Sharp reductions in immunocytochemical staining intensity were observed in the treated animals for a Leydig cell marker, 11beta-hydroxysteroid dehydrogenase, which occurred concurrently with decreased serum T levels. This was consistent with the hypothesis that CORT-mediated induction of apoptosis leads to declines in Leydig cell numbers, thereby affecting T production. These results suggest that excessive exposure to CORT initiates apoptosis in rat Leydig cells, potentially contributing to suppression of circulating T levels during stress and other conditions in which glucocorticoid concentrations are elevated.
authors.affiliation: Laboratory of Reproductive Biology, Shanghai Second Medical University, Shanghai, People's Republic of China.
authors.forename: Hui-Bao
authors.initials: HB
authors.lastname: Gao
authors.forename: Ming-Han
authors.initials: MH
authors.lastname: Tong
authors.forename: Yan-Qiang
authors.initials: YQ
authors.lastname: Hu
authors.forename: Qing-Su
authors.initials: QS
authors.lastname: Guo
authors.forename: Renshan
authors.initials: R
authors.lastname: Ge
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Hardy
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D017209:Apoptosis; D002469:Cell Separation; D002478:Cells, Cultured; D016922:Cellular Senescence; D004396:Coloring Agents; D003345:Corticosterone / Q000494:pharmacology*; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D007985:Leydig Cells / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood; D014343:Trypan Blue
chemical_list: D004396:Coloring Agents; D013739:Testosterone; D007986:Luteinizing Hormone; D004247:DNA; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D014343:Trypan Blue; D003345:Corticosterone