Ovarian follicular wave pattern and the effect of exogenous progesterone on follicular activity in non-mated llamas.
The aim of the present study was two-fold. First, to characterize the secretory profiles of oestradiol-17beta and progesterone in relation to the structural changes observed by ultrasonography during follicular dynamics in non-ovulating llamas. Second, to evaluate the effect of exogenous progesterone on follicular activity, in terms of follicle development and hormone production. In experiment one, six adult non-pregnant, non-lactating llamas were examined daily by rectal palpation and transrectal ultrasonography during 70 days. On day 54, intravaginal devices containing 0.33 g of progesterone (CIDR) were inserted and left in the vagina during 16 days. The mean duration of a follicular wave was 22.6+/-2.5 days. The follicular growth phase (follicles growing from 3mm to maximum size) averaged 9.2+/-2.8 days, the mature phase (follicles around maximum size) 5.2+/-1.4 days and regression phase (follicles with decreasing size) 8.2+/-2.2 days. Oestradiol-17beta plasma concentrations exhibited a similar wave pattern (P<0.05). In addition, oestradiol-17beta peak plasma concentrations (46.9+/-3.3 pmoll(-1)) were attained approximately 12 days after the beginning of the growing phase in connection with maximum follicle size (11.8+/-1.6mm). After CIDR insertion, a rapid increase in plasma progesterone concentrations was observed, with peak concentrations attained on day 1 after insertion. Thereafter, concentrations decreased gradually. Mean follicle size steadily decreased from the day of CIDR insertion to day 11 post-insertion (10.3+/-1.6 and 3.3+/-0.8mm, respectively). In order to investigate the effect of follicle size at CIDR insertion on the outcome of progesterone treatment, experiment two was designed. Sixteen adult non-pregnant and non-lactating llamas were divided into four groups according to follicle development at the time of CIDR insertion (group I: follicles < or =6 mm; group II: follicles between 6 and 9 mm; group III: follicles between 10 and 14 mm and group IV, regressing follicles). In groups II, III and IV, a significant decrease in follicle size was observed after the insertion of the CIDR device. In group I, no further development of dominant follicles was observed until the device was withdrawn. In all cases, the smallest diameter was registered between days 5 and 7 after the beginning of treatment. In conclusion, a detailed characterization of follicular waves using ultrasound and hormone determinations simultaneously in non-ovulating llamas and after the insertion of progesterone releasing devices, is presented.
Chaves
M G
MG
Area de Teriogenología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Chorroarin 280, C.P.1427, Capital Federal, Argentina. chaves@fvet.uba.ar
Aba
M
M
Agüero
A
A
Egey
J
J
Berestin
V
V
Rutter
B
B
D000282:Administration, Intravaginal; D000818:Animals; D002161:Camelids, New World / Q000502:physiology*; D003692:Delayed-Action Preparations; D004958:Estradiol / Q000097:blood*; D005260:Female; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000254:growth & development*; D010060:Ovulation; D011247:Pregnancy; D011374:Progesterone / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D014463:Ultrasonography
D003692:Delayed-Action Preparations; D011374:Progesterone; D004958:Estradiol