Effect of dl-praeruptorin A on ATP sensitive potassium channels in human cortical neurons.
OBJECTIVE
To investigate the effect of dl-praeruptorin A (Pd-Ia) on ATP sensitive potassium channels (KATP channel) in human cortical neurons.


METHODS
Using standard whole cell recording method. Cell membranes were held at -40 mV, commanding potential was -30 to +100 mV and duration was 600 ms.


RESULTS
Pd-Ia activated KATP channels in human cortical neurons in a concentration-dependent manner. After consecutive perfusion with external solution containing Pd-Ia 0.001, 0.01, 0.1, and 1 micromol/L, currents increased from control (0.9 +/- 0.4) nA to (1.0 +/- 0.4) nA, (1.1 +/- 0.4) nA, (1.2 +/- 0.4) nA, and (1.3 +/- 0.4) nA (P < 0.05 or P < 0.01, n = 5) respectively. Then the current decreased to (0.90 +/- 0.37) nA (P < 0.01, n = 5) after washout with glibenclamide (10 micromol/L). The increscent part of the currents could nearly be inhibited by specific KATP channel inhibitor.


CONCLUSIONS
Pd-Ia could open KATP channel and it is a kind of potassium channel opener
Zhang
S L
SL
Department of Pharmacology, China Medical University, Shenyang 110001, China.
Li
J M
JM
Xiao
Q H
QH
Wu
A H
AH
Zhao
Q
Q
Yang
G R
GR
Zhang
K Y
KY
D000255:Adenosine Triphosphate / Q000378:metabolism; D002469:Cell Separation; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D003374:Coumarins / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D009474:Neurons / Q000378:metabolism; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D015221:Potassium Channels; C441070:praeruptorin A; D000255:Adenosine Triphosphate