title: An improved procedure for the synthesis of branched polyethylene glycols (PEGs) with the reporter dipeptide Met-betaAla for protein conjugation.
abstract: A new and more efficient route to the synthesis of branched PEG for protein conjugation, bearing a reporter dipeptide Met-betaAla, is described, which allows better purification of the final product by ion exchange chromatography. The product has the combined advantages of an 'umbrella-like' branched structure, which allows a better coverage of the protein surface, and the presence of the dipeptide Met-betaAla which has been used to detect the position of PEGylation within the peptide sequence.
authors.affiliation: Dipartimento di Science Farmaceutiche, Via Marzolo 5, 35100 Padova, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Guiotto
authors.forename: Michela
authors.initials: M
authors.lastname: Pozzobon
authors.forename: Chiara
authors.initials: C
authors.lastname: Sanavio
authors.forename: Oddone
authors.initials: O
authors.lastname: Schiavon
authors.forename: Piero
authors.initials: P
authors.lastname: Orsolini
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Veronese
mesh_terms: D000409:Alanine / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004151:Dipeptides; D011506:Proteins; D011092:Polyethylene Glycols; D008715:Methionine; D000409:Alanine