Isolation of the major lethal toxin in the venom of Bungarus flaviceps.
The major lethal toxin in the venom of Bungarus flaviceps has been isolated by ion-exchange chromatography, absorption chromatography and RP-HPLC with a 14-fold purification and an overall yield of 16.5% of the lethal toxicity contained in crude venom. Its sublethal dose (LD(50)) determined in mice weighing 18-20 g was 0.25 (0.19-0.32) microg per mouse. The lethal toxin was pure according to disc- and SDS-PAGE as well as gel HPLC. Its apparent molecular weight determined by SDS-PAGE was 29 kDa. It is a basic protein consisting of two polypeptide chains having apparent molecular weights of 17 and 8 kDa, respectively. The toxin has PLA activity but is free of ACE activity.
Khow
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Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama IV Road, 10330, Bangkok, Thailand.
Chanhome
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L
Omori-Satoh
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T
Sitprija
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V
D000818:Animals; D002038:Bungarotoxins / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D017838:Bungarus; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007275:Injections, Intravenous; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry
D002038:Bungarotoxins; D010455:Peptides