Through-bond correlation of sugar and base protons in unlabeled nucleic acids.
This work presents two methods for through-bond correlation between sugar and base protons in view of model-independent assignment in unlabeled or slightly enriched nucleic acids. Each method uses a combination of multiple-bond and one-bond heteronuclear J-couplings to the aromatic carbon C6 for pyrimidines ((3)J(H1',C6) and (1)J(H6,C6)) or C8 for purines ((3)J(H1',C8) and (1)J(H8,C8)). The techniques are demonstrated in the duplex [d(CGCGAATTCGCG)](2) and the dimeric G-quadruplex [d(GGGTTCAGG)](2) at natural abundance.
Phan
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Groupe de Biophysique du Laboratoire de Physique de la Matière Condensée, UMR 7643 du CNRS, Ecole Polytechnique, Palaiseau, 91128, France. pat@pmc.polytechnique.fr
D001483:Base Sequence; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D011522:Protons; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry
D009696:Nucleic Acids; D011522:Protons; D011687:Purines; D011743:Pyrimidines