Inhibitory effect of quercetin on cultured neonatal rat cardiomyocytes hypertrophy induced by angiotensin.
OBJECTIVE
To study the inhibitory effect of quercetin on cultured neonatal rat cardiomyocytes hypertrophy induced by angiotensin II (AngII) and its mechanism.


METHODS
DNA, RNA, and protein synthesis were measured by incorporation of [3H]thymidine, [14C]uridine, and [3H]tyrosine, respectively. Protein content were measured by Lowry's method. Protein kinase C (PKC) activity was assayed by incubating PKC with histone IIIS and [gamma-(32)P]ATP. Tyrosine protein kinase (TPK) activity was assayed by incubating TPK with poly glutamate/tyrosine (4:1) and [gamma-(32P]ATP.


RESULTS
After treated with AngIIat 10 nmol/L for 24 h, total protein content was greatly increased as compared with untreated group (P < 0.01). Incorporation of [14C]uridine and [3H]tyrosine was also greatly increased (P < 0.01) respectively, while incorporation of [3H]thymidine remained unchanged (P > 0.05). Ang II strongly increased cardiomyocytes PKC and TPK activities at 30 min. Que (1-100 micromol/L) could inhibit the increase of total protein content, incorporation of [14C]uridine and [3H]tyrosine, and PKC and TPK activities induced by Ang II in concentration-dependent manner.


CONCLUSIONS
The inhibitory effect of Que on the cardiomyocytes hypertrophy was related to its inhibitory effect on PKC and TPK.
Qin
T C
TC
Department of Pharmacology, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Chen
L
L
Yu
L X
LX
Gu
Z L
ZL
D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D000831:Animals, Newborn; D006332:Cardiomegaly / Q000473:pathology*; D002478:Cells, Cultured; D006984:Hypertrophy; D009206:Myocardium / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000804:Angiotensin II; D011794:Quercetin; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C