title: Modulation of airway responsiveness by anionic and cationic polyelectrolyte substances.
abstract: To elucidate the effects of anionic and cationic polyelectrolyte substance on bronchoconstriction, we examined the serial changes in respiratory resistance (Rrs) in ovalbumin-sensitized guinea pigs after antigen exposure with or without pre-inhalation of low-molecular-weight heparin, poly-L-glutamic acid, poly-L-lysine and dextran, and with or without oral intake of dalteparin. Both immediate and late responses after antigen exposure were significantly decreased after pretreatment with inhaled low-molecular-weight heparin and poly-L-glutamic acid compared with saline alone. The late response was significantly decreased after pretreatment with oral dalteparin. Both low-molecular-weight heparin and poly-L-glutamic acid significantly decreased the airway response to methacholine in sensitized guinea pigs. In sensitized guinea pigs, the airway response to methacholine was significantly increased after pretreatment with inhaled poly-L-lysine. Pretreatment with inhaled low-molecular-weight heparin before poly-L-lysine exposure significantly suppressed the airway hyperresponsiveness after inhaled poly-L-lysine. These findings indicated that the "cationic-anionic interaction" plays an important role in airway responsiveness.
authors.affiliation: Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yahata
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yokoyama
mesh_terms: D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D001249:Asthma / Q000209:etiology; D016084:Bronchoconstriction / Q000187:drug effects*; D005260:Female; D006168:Guinea Pigs; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D011099:Polyglutamic Acid / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology*
chemical_list: D006495:Heparin, Low-Molecular-Weight; D016210:Methacholine Chloride; D011107:Polylysine; D011099:Polyglutamic Acid; D010047:Ovalbumin