An improved procedure for the synthesis of branched polyethylene glycols (PEGs) with the reporter dipeptide Met-betaAla for protein conjugation.
A new and more efficient route to the synthesis of branched PEG for protein conjugation, bearing a reporter dipeptide Met-betaAla, is described, which allows better purification of the final product by ion exchange chromatography. The product has the combined advantages of an 'umbrella-like' branched structure, which allows a better coverage of the protein surface, and the presence of the dipeptide Met-betaAla which has been used to detect the position of PEGylation within the peptide sequence.
Guiotto
Andrea
A
Dipartimento di Science Farmaceutiche, Via Marzolo 5, 35100 Padova, Italy.
Pozzobon
Michela
M
Sanavio
Chiara
C
Schiavon
Oddone
O
Orsolini
Piero
P
Veronese
Francesco M
FM
D000409:Alanine / Q000737:chemistry*; D004151:Dipeptides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004151:Dipeptides; D011506:Proteins; D011092:Polyethylene Glycols; D008715:Methionine; D000409:Alanine