title: Effect of granulocyte colony stimulating factor treatment on ex vivo cytokine production by blood cells of patients after chemotherapy or radiotherapy.
abstract: We explored ex vivo alterations in the cytokine release of stimulated blood cells taken from 8 patients with hematological malignancies who, after chemotherapy or radiotherapy developed leukopenia, and were treated for 3-7 days subcutaneously with granulocyte colony stimulating factor (G-CSF), daily, dose of 5 microg/kg of body weight. Blood was also taken from 8 healthy controls not treated with G-CSF and from patients before and 24 h after last dose of G-CSF and ex vivo treated with interferon (IFN) inducers: Newcastle disease virus (NDV), phytohemagglutinin (PHA), concanavalin A (Con A) and with tumor necrosis factor (TNF) inducer--lipopolysaccharide (LPS). Blood cells of patients before G-CSF treatment exhibited ex vivo a low ability to produce IFN-gamma in comparison to controls. After G-CSF therapy a significant increase in IFN-alpha production ability was detected. We conclude that G-CSF treatment for 3-7 days does not only increase the number of white blood cells (WBC) and neutrophilic granulocytes but also modify the host response of patients with hematological malignancies to microbial infections.
authors.affiliation: Department of Virology and Immunology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Kamińska
authors.forename: A
authors.initials: A
authors.lastname: Dmoszyńska
authors.forename: I
authors.initials: I
authors.lastname: Hus
authors.forename: A W
authors.initials: AW
authors.lastname: Croneck
authors.forename: M
authors.initials: M
authors.lastname: Kandefer-Szerszeń
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects* / Q000378:metabolism; D019337:Hematologic Neoplasms / Q000150:complications* / Q000188:drug therapy / Q000628:therapy; D006801:Humans; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000188:drug therapy* / Q000276:immunology; D011994:Recombinant Proteins
chemical_list: D016207:Cytokines; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor