Effects of Tripterygium wilfordii saponins and interleukin-10 on dendritic cells from human peripheral blood.
OBJECTIVE
To study the effects of Tripterygium wilfordii (TII) and IL-10 on human leukocyte antigen-DR (HLA-DR) and CD80 expressions and IL-12 p40 subunit production and transcription of dendritic cells (DC) in human peripheral blood from healthy volunteers in vitro.


METHODS
DC were generated by culturing plastic-adherent peripheral blood mononuclear cells with GM-colony stimulating factor (GM-CSF), IL-4, and TNFalpha. The expressions of HLA-DR and CD80 were examined by flow cytometry after the cells were stained with immunofluorescence antibodies. Enzyme-linked immunosorbent assay and reverse transcriptase polymerase chain reaction analysis were used to detect IL-12 p40 protein level and mRNA transcription, respectively.


RESULTS
TII 5 - 20 mg/L and IL-10 50 - 200 microg/L greatly down-regulated the membrane expressions of HLA-DR and CD80 on DC in a concentration-dependent manner. IL-12 p40 production and mRNA transcription were also inhibited in DC both by TII and by IL-10.


CONCLUSIONS
TII and IL-10 exert immunosuppressive role via inhibiting membrane expressions of HLA-DR and CD80 and synthesis of IL-12 p40 subunit in DC.
Wang
S J
SJ
Department of Microbiology and Immunology, Zhenjiang Medical College, Zhenjiang 212001, China. shengjwang@263.net
Yao
K
K
Xie
F D
FD
Ji
X H
XH
D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D012503:Saponins / Q000494:pharmacology*; D029757:Tripterygium / Q000737:chemistry*
D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D007166:Immunosuppressive Agents; D012503:Saponins; D016753:Interleukin-10