Determination of bisphenol A and 10 alkylphenols in serum using SDS micelle capillary electrophoresis with gamma-cyclodextrin.
A new micelle capillary electrophoresis based on cyclodextrin micellar electrokinetic chromatography (MEKC) for the determination of bisphenol A and 10 alkylphenols in rat serum is reported. Several surfactants and dextrins were studied. Bisphenol A and alkylphenols were separated using a 50 microm x 50 cm capillary with 20 mM borate phosphate buffer (pH 8.0) containing 20 mM sodium dodecylsulfate and 5 mM gamma-cyclodextrin as carrier. The method could determine 0.6-2000 microg/mL of phenols in 100 microL serum by photometric detection at 214 nm. Using 2.0 mL serum, 1.0 ng/mL of phenols could be determined. The relative standards deviations were 6.3-7.7% at 10 microg/mL in serum. The recoveries were 91.8-93.0% with 10 microg/mL serum samples.
Katayama
M
M
Department of Functional Bioanalysis, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan.
Matsuda
Y
Y
Sasaki
T
T
Shimokawa Ki
K
K
Kaneko
S
S
Iwamoto
T
T
D001559:Benzhydryl Compounds; D001881:Borates; D002021:Buffers; D002793:Cholic Acids; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010636:Phenols / Q000097:blood*; D010710:Phosphates; D011786:Quality Control; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents; D047408:gamma-Cyclodextrins; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D001881:Borates; D002021:Buffers; D002793:Cholic Acids; D003505:Cyclodextrins; D007202:Indicators and Reagents; D010636:Phenols; D010710:Phosphates; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents; D047408:gamma-Cyclodextrins; C023792:gamma-cyclodextrin; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate