title: Expansion in scid mice of Epstein-Barr virus-associated post-transplantation lymphoproliferative disease biopsy material.
abstract: Post-transplant lymphoproliferative disease (PTLD) biopsy material is rarely available in adequate quantity for research. Therefore, the present study was designed to expand biopsy material in scid mice. Epstein-Barr virus (EBV)+ve PTLD samples from five transplant patients were established in scid mice. PCR analysis of immunoglobulin gene rearrangements demonstrated that four of the five biopsies (80%) gave rise to scid tumours which represented the original tumour cell clones. Immunophenotyping showed that these four biopsies (and all scid tumours) expressed all EBV latent genes and a B lymphoblast phenotype; <or=26% T cells were found in the biopsy material whereas scid tumours showed a paucity of T lymphocytes. RT-PCR analysis revealed expression of IL-2, -4, -6, -10 and IFN-gamma in all tumour material, suggesting key roles for these factors in tumour growth. The results show that EBV+ve PTLD material can be expanded in scid mice giving rise to quantities of homogeneous malignant tissue sufficient for research studies.
authors.affiliation: Laboratory for Clinical and Molecular Virology, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK1.
authors.forename: Ingolfur
authors.initials: I
authors.lastname: Johannessen
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK2.
authors.forename: Sunimali M
authors.initials: SM
authors.lastname: Perera
authors.affiliation: Leukaemia Research Fund Virus Centre, University of Glasgow, Bearsden, Glasgow G61 1QH, UK3.
authors.forename: Alice
authors.initials: A
authors.lastname: Gallagher
authors.affiliation: Laboratory for Clinical and Molecular Virology, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK1.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Hopwood
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK2.
authors.forename: J Alero
authors.initials: JA
authors.lastname: Thomas
authors.affiliation: Laboratory for Clinical and Molecular Virology, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK1.
authors.forename: Dorothy H
authors.initials: DH
authors.lastname: Crawford
mesh_terms: D000293:Adolescent; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis; D005260:Female; D015870:Gene Expression; D016027:Heart Transplantation / Q000009:adverse effects*; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007223:Infant; D007244:Infectious Mononucleosis / Q000276:immunology / Q000473:pathology / Q000821:virology*; D016030:Kidney Transplantation / Q000009:adverse effects*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000821:virology; D017455:Receptors, IgE / Q000032:analysis; D015534:Trans-Activators / Q000032:analysis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000032:analysis; D014764:Viral Proteins
chemical_list: D018941:Antigens, CD19; D000956:Antigens, Viral; C063676:BZLF1 protein, Herpesvirus 4, Human; D016207:Cytokines; D004268:DNA-Binding Proteins; C402641:EBNA-2 protein, Human herpesvirus 4; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D017455:Receptors, IgE; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C417029:EBV-encoded nuclear antigen 1