Multi-copy repression of Serratia marcescens nuclease expression by dinI.
The dinI homolog of S. marcescens was cloned from a plasmid library by virtue of its ability to inhibit nuclease expression from the S. marcescens nucA gene integrated in the genome of E. coli. The S. marcescens DinI protein is 68% identical to DinI of E. coli. It has a similar effect on other SOS regulated genes and likely exerts it effect on nuclease expression, which is most pronounced as the cells entered stationary phase, through inhibition of basal SOS expression.
Berkmen
Mehmet
M
Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA.
Benedik
Michael J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D004720:Endonucleases / Q000235:genetics*; D015870:Gene Expression; D008836:Micrococcal Nuclease; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D010957:Plasmids / Q000096:biosynthesis; D013014:SOS Response, Genetics; D016415:Sequence Alignment; D012706:Serratia marcescens / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D010940:Plant Proteins; C111803:din1 protein, Raphanus sativus; C074002:nuc protein, staphylococcus; D004720:Endonucleases; D008836:Micrococcal Nuclease