Allitridum mimics effect of ischemic preconditioning by activation of protein kinase C.
OBJECTIVE
To investigate whether allitridum has the effect of pharmacological preconditioning and whether protein kinase C (PKC) plays a role in myocardial protection.


METHODS
Thirty-four isolated rabbit hearts which subjected to 30 min of regional myocardial ischemia and 2 h reperfusion, were randomly divided into 5 groups: control group, ischemic preconditioning (PC) group, allitridum (A) group, polymyxin B (Poly B) group, allitridum + polymyxin B (A + Poly B) group. Infarct size was determined by triphenyltetrazolium staining.


RESULTS
Pharmacological preconditioning in hearts with a 5 -min allitridum infusion 10 min before the prolonged regional ischemia resulted in significantly smaller infarcts (7 % +\- 6 % of risk area) than in control hearts (25 % +\- 7 %, P < 0.05). There is no significant difference in infarct size between (A+Poly B) group and control hearts (23 % +\- 5 % vs 25 % +\- 7 %, P > 0.05).


CONCLUSIONS
These data indicate that allitridum can precondition rabbit ischemic myocardium and this protection can be effectively blocked by administration of Poly B, an inhibitor of PKC, implying that PKC has an important role in preconditioning.
Zhang
W J
WJ
Department of Heart and Kidney, China-Japan Friendship Hospital, Beijing 100029, China.
Shi
Z X
ZX
Wang
B B
BB
Cui
Y J
YJ
Guo
J Z
JZ
Li
B
B
D000498:Allyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation; D005260:Female; D005737:Garlic / Q000737:chemistry; D066298:In Vitro Techniques; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D017202:Myocardial Ischemia / Q000473:pathology; D011112:Polymyxin B / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology; D011817:Rabbits; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*
D000498:Allyl Compounds; D013440:Sulfides; C042577:diallyl trisulfide; D011493:Protein Kinase C; D011112:Polymyxin B