Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.
To explore the physiological roles of sulfotransferases (SULTs) in extra-hepatic tissues, we examined the expression of eight SULT genes by reverse transcription (RT)-PCR in human cell lines that were established from various tissues. Expression levels of SULTs were low in neural cell lines such as NB-1 and GI-1, and high in epithelial cell lines, such as Caco-2 and BeWo. SULT1C2 expression was abundant in all cell types, whereas that of SULT1E1, SULTIBI or SULT2B1 was restricted to a specific cell type. SULT1C1, which can catalyze the sulfation of N-hydroxy-2-acetylaminofluorene, was expressed in Caco-2, BeWo and KB562. Induction of differentiation did not generally affect SULT expression, although that of SULT1C2 was reduced after differentiation of the neuroblastoma cell line, NB-1, was induced. The profile of SULT expression in the culture cells obtained here gives clues to understanding the physiological roles of SULT enzymes in extra-hepatic tissues or organs.
Tamura
H O
HO
Kyoritsu College of Pharmacy, Tokyo, Japan. tamura-hr@kyoritsu-ph.ac.jp
Taniguchi
K
K
Hayashi
E
E
Hiyoshi
Y
Y
Nagai
F
F
D018938:Caco-2 Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002822:Choriocarcinoma / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology; D004298:Dopamine / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018922:HL-60 Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D020014:K562 Cells / Q000166:cytology / Q000378:metabolism*; D009284:Naphthols / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000201:enzymology; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D014594:Uterine Neoplasms / Q000201:enzymology
D007527:Isoenzymes; D009284:Naphthols; D015238:Sulfotransferases; C433248:SULT2B1 protein, human; C028405:2-naphthol; D004298:Dopamine