title: Molecular mechanisms involved in mammalian gamete fusion.
abstract: Fusion between gametes is a key event in the fertilization process involving the interaction of specific domains of the sperm and egg plasma membranes. During recent years, efforts have been made toward the identification of the specific molecular components involved in this event. The present work will focus on the best characterized candidates for mediating gamete membrane fusion in mammals. These molecules include members of the ADAM (a disintegrin and a metalloprotease domain) family, i.e., testicular proteins fertilin alpha, fertilin beta, and cyritestin, which are thought to interact with integrins in the egg plasma membrane through their disintegrin domains, and a member of the cysteine-rich secretory proteins (CRISP) family, i.e., epididymal protein DE, which participates in an event subsequent to sperm-egg binding and leading to fusion through specific complementary sites localized on the fusogenic area of the egg surface. The identification and characterization of these molecules will contribute not only to a better understanding of the molecular mechanisms underlying mammalian sperm-egg fusion but also to the development of new methods for both fertility regulation and diagnosis and treatment of human infertility.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME), Buenos Aires, Argentina. cuasnicu@dna.uba.ar
authors.forename: P S
authors.initials: PS
authors.lastname: Cuasnicú
authors.forename: D A
authors.initials: DA
authors.lastname: Ellerman
authors.forename: D J
authors.initials: DJ
authors.lastname: Cohen
authors.forename: D
authors.initials: D
authors.lastname: Busso
authors.forename: M M
authors.initials: MM
authors.lastname: Morgenfeld
authors.forename: V G
authors.initials: VG
authors.lastname: Da Ros
mesh_terms: D051722:ADAM Proteins; D000818:Animals; D006224:Cricetinae; D004527:Egg Proteins / Q000502:physiology; D005260:Female; D000072196:Fertilins; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D008297:Male; D008322:Mammals / Q000235:genetics / Q000502:physiology; D008561:Membrane Fusion; D008562:Membrane Glycoproteins / Q000502:physiology; D008647:Mesocricetus; D008666:Metalloendopeptidases / Q000502:physiology; D051379:Mice; D051381:Rats; D012471:Salivary Proteins and Peptides / Q000502:physiology; D029589:Seminal Plasma Proteins / Q000502:physiology; D013045:Species Specificity; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D022642:Vaccines, Contraceptive; D015044:Zona Pellucida / Q000502:physiology
chemical_list: C103538:CRISP1 protein, human; C486321:Crisp1 protein, mouse; C486320:Crisp3 protein, rat; D004527:Egg Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins; D022642:Vaccines, Contraceptive; D051722:ADAM Proteins; C000606454:ADAM2 protein, human; C499566:ADAM3A protein, human; C000606455:Adam1a protein, mouse; C000606456:Adam1a protein, rat; C000606458:Adam2 protein, mouse; C000606459:Adam2 protein, rat; C485429:Adam3 protein, mouse; D000072196:Fertilins; D008666:Metalloendopeptidases