Antiinflammatory activity of the antirheumatic herbal drug, gravel root (Eupatorium purpureum): further biological activities and constituents.
Previous reports from this laboratory revealed that cistifolin from the antirheumatic herbal drug, gravel root (rhizome of Eupatorium purpureum), showed activity both in in vitro and in vivo models of inflammation. Data are now presented to show that, in addition to the LFA-1 and other integrin-mediated leucocyte adhesions, cistifolin inhibits the Mac-1 (CD11b/CD18)-dependent monocyte adhesion to fibrinogen in a concentration-dependent manner. Further phytochemical analysis of the crude extract also led to the isolation of three known benzofurans, euparin, euparone, 6-hydroxy-3beta-methoxytrematone and a new benzofuran, 5-acetyl-6-hydroxy-2-(1-oxo-2-acetoxy-ethyl)-benzofuran. None of these compounds were active in suppressing integrin-mediated monocytic U937 cell adhesions in vitro.
Habtemariam
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School of Chemical and Life Sciences, University of Greenwich, Wellington Street, Woolwich, London SE18 6PF, UK.
D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D019659:Asteraceae; D001572:Benzofurans / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000166:cytology; D005340:Fibrinogen / Q000187:drug effects; D006801:Humans; D016177:Macrophage-1 Antigen / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology; D018517:Plant Roots; D018514:Plant Structures
D000893:Anti-Inflammatory Agents; D001572:Benzofurans; D016177:Macrophage-1 Antigen; D010936:Plant Extracts; C117515:cistifolin; D005340:Fibrinogen