Removal of poliovirus type 1 from a protein mixture using an immunoaffinity chromatography column.
An immunoaffinity matrix was prepared using human polyclonal antibody (Intragam) attached to Sepharose 4B activated with CNBr. The immunoaffinity matrix was then assessed with regard to its capacity to remove viruses. The challenge virus, poliovirus type 1 was loaded in high titre in either PBS or a preparation derived from human plasma known as supernatant II + III. This fraction is depleted of IgG and is used to prepare human albumin. It was shown that an average greater than 5 logs of spiked virus were removed in one passage through the column. This type of approach may prove useful as a viral removal method in biopharmaceutical manufacturing.
Cameron-Smith
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Department of Infectious Diseases, University of Sydney, NSW 2006, Australia.
Harbour
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D000914:Antibodies, Viral; D002846:Chromatography, Affinity / Q000379:methods*; D002847:Chromatography, Agarose; D003488:Cyanogen Bromide; D006367:HeLa Cells; D006801:Humans; D007158:Immunologic Techniques; D017955:Poliovirus / Q000276:immunology* / Q000302:isolation & purification*; D011506:Proteins; D012996:Solutions
D000914:Antibodies, Viral; D011506:Proteins; D012996:Solutions; D003488:Cyanogen Bromide