title: Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation.
abstract: Six patients received an allogeneic bone marrow transplant from HLA-identical ABO-mismatched donors. ABO genotype of erythroid burst-forming units (BFU-E) from peripheral blood was analyzed using polymerase chain reaction with sequence specific primers (PCR-SSP). After bone marrow transplantation (BMT), engraftment of donor cells by ABO genotypic analysis of BFU-E was compared with ABO phenotypic analysis of red blood cells (RBCs). During the early stage after BMT, ABO genotype of BFU-E in the recipients converted to that of the donors. In contrast, mixed ABO phenotype of RBCs persisted for about 3 months. In one patient, autologous hemopoietic cell recovery was detected by the ABO genotypic analysis before clinical manifestation. ABO genotypic analysis of BFU-E is relevant for enagraftment after ABO-mismatched BMT.
authors.affiliation: Division of Cell Transplantation and Transfusion, Jichi Medical School, Minamikawachi, 329-0498, Tochigi, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Kishino
authors.forename: Kazuo
authors.initials: K
authors.lastname: Muroi
authors.forename: Chizuru
authors.initials: C
authors.lastname: Kawano
authors.forename: Takashi
authors.initials: T
authors.lastname: Obata
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugano
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakagi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagashima
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Watari
authors.forename: Sadahiko
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D017931:DNA Primers / Q000737:chemistry; D004912:Erythrocytes / Q000166:cytology; D015672:Erythroid Precursor Cells / Q000502:physiology*; D004920:Erythropoiesis; D005434:Flow Cytometry; D005838:Genotype; D006085:Graft Survival; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000628:therapy*; D015466:Leukemia, Myeloid, Chronic-Phase / Q000097:blood / Q000628:therapy*; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000628:therapy*
chemical_list: D000017:ABO Blood-Group System; D017931:DNA Primers