Nomofungin: a new microfilament disrupting agent.
A new alkaloid, nomofungin, has been isolated from the fermentation broth of an unidentified endophytic fungus obtained from the bark of Ficus microcarpa L. The structure of nomofungin was determined by application of spectroscopic methods. The absolute stereochemistry of nomofungin was assigned by using the exciton chirality method. Nomofungin disrupts microfilaments in cultured mammalian cells and is moderately cytotoxic with minimum inhibitory concentrations (MICs) of 2 and 4.5 microg/mL against LoVo and KB cells, respectively. The ring system of nomofungin is unprecedented.
Ratnayake
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Department of Chemistry, University of Hawaii at Manoa, 2545 McCarthy Mall, Honolulu, HI 96822-2275, USA.
Yoshida
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Mooberry
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Hemscheidt
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TK
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004354:Drug Screening Assays, Antitumor; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology*; D005285:Fermentation; D030681:Ficus / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D005658:Fungi / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008840:Microfilament Proteins / Q000378:metabolism*; D008958:Models, Molecular; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D024301:Plant Bark / Q000382:microbiology; D051381:Rats; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D000903:Antibiotics, Antineoplastic; D004852:Epoxy Compounds; D008840:Microfilament Proteins; C445785:nomofungin