Selective projections of cholecystokinin-8 immunoreactive fibers to galanin immunoreactive sympathetic preganglionic neurons in a teleost, Stephanolepis cirrhifer.
In the cellular column of sympathetic preganglionic neurons (SPNs) of the filefish Stephanolepis cirrhifer, neurons containing galanin (GAL) form a distinct population projecting specifically to non-adrenergic postganglionic neurons in the celiac and cranial sympathetic ganglia. The present study showed that virtually all of the GAL-immunopositive SPNs made contact with many nerve terminals immunopositive for cholecystokinin octapeptide (CCK-8). GAL-negative preganglionic neurons made contact with only 26% of this type of nerve terminal; CCK-8-immunopositive nerve fibers appeared to project selectively to GAL-immunopositive SPNs with projections to specific targets. The CCK-8-positive nerve fibers might be of primary sensory origin, and participate in the visceral reflexes.
Funakoshi
K
K
Department of Anatomy, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. funako@med.yokohama-cu.ac.jp
Nakano
M
M
Atobe
Y
Y
Kadota
T
T
Goris
R C
RC
Kishida
R
R
D000109:Acetylcholine / Q000378:metabolism; D000344:Afferent Pathways / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D004525:Efferent Pathways / Q000166:cytology / Q000378:metabolism*; D005399:Fishes / Q000033:anatomy & histology / Q000378:metabolism*; D019004:Galanin / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013126:Spinal Nerve Roots / Q000378:metabolism / Q000648:ultrastructure
D012701:Serotonin; D019004:Galanin; D002766:Cholecystokinin; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine