Genes required for ionizing radiation resistance in yeast.
The ability of Saccharomyces cerevisiae to tolerate ionizing radiation damage requires many DNA-repair and checkpoint genes, most having human orthologs. A genome-wide screen of diploid mutants homozygous with respect to deletions of 3,670 nonessential genes revealed 107 new loci that influence gamma-ray sensitivity. Many affect replication, recombination and checkpoint functions. Nearly 90% were sensitive to other agents, and most new genes could be assigned to the following functional groups: chromatin remodeling, chromosome segregation, nuclear pore formation, transcription, Golgi/vacuolar activities, ubiquitin-mediated protein degradation, cytokinesis, mitochondrial activity and cell wall maintenance. Over 50% share homology with human genes, including 17 implicated in cancer, indicating that a large set of newly identified human genes may have related roles in the toleration of radiation damage.
Bennett
C B
CB
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.
Lewis
L K
LK
Karthikeyan
G
G
Lobachev
K S
KS
Jin
Y H
YH
Sterling
J F
JF
Snipe
J R
JR
Resnick
M A
MA
D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D005720:Gamma Rays; D005800:Genes, Fungal; D009154:Mutation; D011003:Ploidies; D011836:Radiation Tolerance / Q000235:genetics*; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects*
D017931:DNA Primers