Investigation of glutathione S-transferase zeta and the development of sporadic breast cancer.
BACKGROUND
Certain genes from the glutathione S-transferase superfamily have been associated with several cancer types. It was the objective of this study to determine whether alleles of the glutathione S-transferase zeta 1 (GSTZ1) gene are associated with the development of sporadic breast cancer.


METHODS
DNA samples obtained from a Caucasian population affected by breast cancer and a control population, matched for age and ethnicity, were genotyped for a polymorphism of the GSTZ1 gene. After PCR, alleles were identified by restriction enzyme digestion and results analysed by chi-square and CLUMP analysis.


RESULTS
Chi-squared analysis gave a chi2 value of 4.77 (three degrees of freedom) with P = 0.19, and CLUMP analysis gave a T1 value of 9.02 with P = 0.45 for genotype frequencies and a T1 value of 4.77 with P = 0.19 for allele frequencies.


CONCLUSIONS
Statistical analysis indicates that there is no association of the GSTZ1 variant and hence the gene does not appear to play a significant role in the development of sporadic breast cancer.
Smith
R A
RA
Genomics Research Centre, Griffith University Gold Coast, Southport, Queensland, Australia. L.Griffiths@mailbox.gu.edu.au
Curran
J E
JE
Weinstein
S R
SR
Griffiths
L R
LR
D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D017931:DNA Primers; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics
D017931:DNA Primers; C407705:GSTZ1 protein, human; D005982:Glutathione Transferase