Enhanced anti-Shigella activity of erythromycin supplemented with sulfadiazine.
Sulfadiazine enhanced the anti-Shigella activity of erythromycin. Erythromycin passes through the type III secretion apparatus and suppresses the growth of invasive Shigella organisms. Sulfadiazine enhanced this effect at the concentration under minimum inhibitory concentration and it came from not only the folate-inhibiting activity but also from a new function. It has proved that sulfadiazine stimulated type III secretion in Shigella as determined from the secretion of the pathogenic protein IpaB. As Congo red induced secretion of Ipa proteins and uptake of erythromycin through the type III secretion gate, sulfadiazine which is similar to Congo red in chemical structure may induce the uptake in the same way.
Honma
Y
Y
Department of Microbiology, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan. yhonma@fujita-hu.ac.jp
Sasakawa
C
C
Tsuji
T
T
Iwanaga
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D004357:Drug Synergism; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D012760:Shigella / Q000187:drug effects* / Q000254:growth & development; D013411:Sulfadiazine / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D013411:Sulfadiazine; C087447:ipaB protein, Shigella; D003224:Congo Red; D004917:Erythromycin