title: Cytokine pattern in aneurysmal and occlusive disease of the aorta.
abstract: BACKGROUND
  Prominent inflammatory infiltrates of macrophages and T-lymphocytes are found in both aortic occlusive disease (AOD) and abdominal aortic aneurysms (AAA). These cells secrete different cytokines that might affect matrix turnover through modulation of matrix metalloproteinase expression. A different cytokine pattern might account for the evolution of AOD vs AAA.
  
  
  METHODS
  Six different cytokines were examined to determine whether AOD and AAA could be characterized by unique cytokine patterns. AOD (n = 8) and AAA (n = 8) tissues were collected and serially treated with salt, dimethyl sulfoxide, and urea buffers to extract the soluble matrix or cell-bound cytokines. Levels of IL-1 beta, TNF-alpha, IL-10, IL-12, and IFN-gamma were measured by immunoenzymatic methods. Additionally, RNA levels of IL-12 and IFN-gamma were measured.
  
  
  RESULTS
  AAA tissue contained higher levels of IL-10 compared to AOD tissue (P < 0.05). Higher levels of the proinflammatory cytokines IL-1 beta, TNF-alpha, and IL-6 were found in AOD (P < 0.05). mRNA levels of IL-12 and IFN-gamma did not differ between the diseases. Aortic tissues contained large amounts of matrix or cell-bound cytokines.
  
  
  CONCLUSIONS
  AAA is characterized by greater levels of IL-10 while IL-1 beta, TNF-alpha, and IL-6 are higher in AOD. Targeted deletion of these cytokines in animal models might help in identifying their role in the progression of AAA.
authors.affiliation: Department of Surgery, University of Nebraska College of Medicine, 983280 Nebraska Health System, Omaha, Nebraska 68198, USA.
authors.forename: V A
authors.initials: VA
authors.lastname: Davis
authors.forename: R N
authors.initials: RN
authors.lastname: Persidskaia
authors.forename: L M
authors.initials: LM
authors.lastname: Baca-Regen
authors.forename: N
authors.initials: N
authors.lastname: Fiotti
authors.forename: B G
authors.initials: BG
authors.lastname: Halloran
authors.forename: B T
authors.initials: BT
authors.lastname: Baxter
mesh_terms: D017544:Aortic Aneurysm, Abdominal / Q000276:immunology*; D001018:Aortic Diseases / Q000276:immunology*; D001157:Arterial Occlusive Diseases / Q000276:immunology*; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10