A compared study on the purification of fetal and adult rat hepatocyte suspensions by biomagnetic isolation techniques and flow cytometry analysis.
An analysis of liver cell populations from both adult and 21 day pregnancy rat fetuses (E21) was carried out. The results show that E21 hepatocytes express OX-43, as do endothelial cells but not adult hepatocytes. OX-43 could be used in future as a cell marker for the hepatocyte maturation.
Arza
E
E
Departamento de Biología Animal II, Universidad Complutense, 28040 Madrid, Spain.
Cubero
F J
FJ
García-Barrutia
M S
MS
Ortiz
A
A
Maganto
P
P
Mula
N
N
Arahuetes
R M
RM
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D022781:Hepatocytes / Q000166:cytology*; D018189:Immunomagnetic Separation; D008099:Liver / Q000166:cytology* / Q000196:embryology; D008297:Male; D018613:Microscopy, Confocal; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal