Using spectrophotometry and spectral mapping technique for the study of the production of manganese-dependent and manganese-independent peroxidases by Pleurotus ostreatus.
The activity of manganese-dependent and manganese-independent peroxidases produced by Pleurotus ostreatus in culture media composed of agro-residues was measured by visible spectrophotometry. The overall enzyme activity and its selectivity were separated by using spectral mapping technique followed by nonlinear mapping. The relationships between the parameters of enzyme production and the composition of culture media and fermentation time was assessed by stepwise regression analysis. Calculations proved that the addition of extract of straw to the culture media significantly decreased the overall production of both enzymes, whereas the selectivity of enzyme production was influenced by amount of potato extract and the concentration of total sugar in the culture media. Enzyme activity depended quadratically on the fermentation time.
Morais
Helena
H
National Agronomical Station, Quinta do Marques, 2784-505 Oeiras, Portugal.
Ramos
Cristina
C
Forgács
Esther
E
Cserháti
Tibor
T
Oliviera
José
J
D003470:Culture Media; D005285:Fermentation; D015999:Multivariate Analysis; D010544:Peroxidases / Q000032:analysis* / Q000096:biosynthesis*; D020076:Pleurotus / Q000201:enzymology*; D013053:Spectrophotometry / Q000379:methods*
D003470:Culture Media; D010544:Peroxidases; C051129:manganese peroxidase