title: Determination of midazolam in human plasma by solid-phase microextraction and gas chromatography/mass spectrometry.
abstract: A new method is described for the qualitative and quantitative analysis of midazolam, a short-acting 1,4-imidazole benzodiazepine, in human plasma. It involves a plasma deproteinization step, solid-phase microextraction (SPME) of midazolam using an 85-microm polyacrylate fiber, and its detection by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring (SIM) mode, using pinazepam as internal standard. The assay is linear over a midazolam plasma range of 1.5-300 ng/mL, relative intra- and inter-assay standard deviations at 5 ng/mL are below 7%, and the limit of detection is 1 ng/mL. The method is simple, fast and sufficiently sensitive to be applied in clinical and forensic toxicology as well as for purposes of therapeutic drug monitoring.
authors.affiliation: Forensic Toxicology and Antidoping, University Hospital of Padova, Via Falloppio 50, I-35121 Padova, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Frison
authors.forename: L
authors.initials: L
authors.lastname: Tedeschi
authors.forename: S
authors.initials: S
authors.lastname: Maietti
authors.forename: S D
authors.initials: SD
authors.lastname: Ferrara
mesh_terms: D014151:Anti-Anxiety Agents / Q000097:blood*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D008874:Midazolam / Q000097:blood*
chemical_list: D014151:Anti-Anxiety Agents; D006993:Hypnotics and Sedatives; D008874:Midazolam