title: Effect of glutamine and GABA on [U-(13)C]glutamate metabolism in cerebellar astrocytes and granule neurons.
abstract: To probe the effect of glutamine and GABA on metabolism of [U-(13)C]glutamate, cerebellar astrocytes were incubated with [U-(13)C]glutamate (0.5 mM) in the presence and absence of glutamine (2.5 mM) or GABA (0.2 mM). It could be shown that consumption of [U-(13)C]glutamate was decreased in the presence of glutamine and release of labeled aspartate and [1,2,3-(13)C]glutamate decreased as well, whereas the concentrations of these metabolites increased inside the cells. Glutamine decreased energy production from [U-(13)C]glutamate presumably by substituting for glutamate as an energy substrate. No additional effect was seen in the presence of both glutamine and GABA. When cerebellar granule neurons were incubated with [U-(13)C]glutamate (0.25 mM) and GABA (0.05 mM), less [U-(13)C]glutamate was used for energy production than in controls. Because the barbiturate thiopental did not elicit such response (Qu et al., 2000, Neurochem Int 37:207-215) it appears that GABA also has a metabolic function in the glutamatergic cerebellar granule neurons in contrast to the astrocytes.
authors.affiliation: Department of Clinical Neuroscience, Norwegian University of Science and Technology, Olav Kyrresgt. 3, N-7489 Trondheim, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Qu
authors.forename: J R
authors.initials: JR
authors.lastname: Konradsen
authors.forename: M
authors.initials: M
authors.lastname: van Hengel
authors.forename: S
authors.initials: S
authors.lastname: Wolt
authors.forename: U
authors.initials: U
authors.lastname: Sonnewald
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002952:Citric Acid Cycle / Q000502:physiology; D004347:Drug Interactions / Q000502:physiology; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D002457:Cell Extracts; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005978:Glutathione