Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer.
Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.
Stockwin
Luke H
LH
School of Biochemistry and Molecular Biology, Mount Preston Street, University of Leeds, Leeds LS2 9JT, UK.
Matzow
Torkjel
T
Georgopoulos
Nikolaos T
NT
Stanbridge
Lindsay J
LJ
Jones
Samantha V
SV
Martin
Iain G
IG
Blair-Zajdel
Maria E
ME
Blair
G Eric
GE
D017951:Antigen Presentation / Q000235:genetics* / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology; D011991:Receptors, Virus / Q000235:genetics* / Q000276:immunology; D014162:Transfection / Q000379:methods
D011991:Receptors, Virus; C085301:adenovirus receptor; C105941:coxsackievirus B receptor