title: The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice.
abstract: Identification of mutations in the ABCA1 transporter (ABCA1) as the genetic defect in Tangier disease has generated interest in modulating atherogenic risk by enhancing ABCA1 gene expression. To investigate the role of ABCA1 in atherogenesis, we analyzed diet-induced atherosclerosis in transgenic mice overexpressing human ABCA1 (hABCA1-Tg) and spontaneous lesion formation in hABCA1-Tg x apoE-knockout (KO) mice. Overexpression of hABCA1 in C57BL/6 mice resulted in a unique anti-atherogenic profile characterized by decreased plasma cholesterol (63%), cholesteryl ester (63%), free cholesterol (67%), non-high density lipoprotein (HDL)-cholesterol (53%), and apolipoprotein (apo) B (64%) but markedly increased HDL-cholesterol (2.8-fold), apoA-I (2.2-fold), and apoE (2.8-fold) levels. These beneficial changes in the lipid profile led to significantly lower (65%) aortic atherosclerosis in hABCA1-Tg mice. In marked contrast, ABCA1 overexpression had a minimal effect on the plasma lipid profile of apoE-KO mice and resulted in a 2- to 2.6-fold increase in aortic lesion area. These combined results indicate that overexpression of ABCA1 in C57BL/6 mice on a high cholesterol diet results in an atheroprotective lipoprotein profile and decreased atherosclerosis, and thus provide previously undocumented in vivo evidence of an anti-atherogenic role for the ABCA1 transporter. In contrast, overexpression of ABCA1 in an apoE-KO background led to increased atherosclerosis, further substantiating the important role of apoE in macrophage cholesterol metabolism and atherogenesis. In summary, these results establish that, in the presence of apoE, overexpression of ABCA1 modulates HDL as well as apoB-containing lipoprotein metabolism and reduces atherosclerosis in vivo, and indicate that pharmacological agents that will increase ABCA1 expression may reduce atherogenic risk in humans.
authors.affiliation: Molecular Disease Branch, National Heart, Lung, and Blood Institute, Bethesda, MD 20892, USA. cjoyce@mail.nih.gov
authors.forename: Charles W
authors.initials: CW
authors.lastname: Joyce
authors.forename: Marcelo J A
authors.initials: MJ
authors.lastname: Amar
authors.forename: Gilles
authors.initials: G
authors.lastname: Lambert
authors.forename: Boris L
authors.initials: BL
authors.lastname: Vaisman
authors.forename: Beverly
authors.initials: B
authors.lastname: Paigen
authors.forename: Jamila
authors.initials: J
authors.lastname: Najib-Fruchart
authors.forename: Robert F
authors.initials: RF
authors.lastname: Hoyt
authors.forename: Edward D
authors.initials: ED
authors.lastname: Neufeld
authors.forename: Alan T
authors.initials: AT
authors.lastname: Remaley
authors.forename: Donald S
authors.initials: DS
authors.lastname: Fredrickson
authors.forename: H Bryan
authors.initials: HB
authors.lastname: Brewer
authors.forename: Silvia
authors.initials: S
authors.lastname: Santamarina-Fojo
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D001011:Aorta / Q000473:pathology*; D016632:Apolipoprotein A-I / Q000096:biosynthesis; D001057:Apolipoproteins E / Q000096:biosynthesis / Q000235:genetics*; D001161:Arteriosclerosis / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood; D003433:Crosses, Genetic; D004032:Diet; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011485:Protein Binding; D012737:Sex Factors
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D016632:Apolipoprotein A-I; D001057:Apolipoproteins E; D008076:Cholesterol, HDL; D002784:Cholesterol