title: [A shortened exposure of oocytes to sperm in standard in vitro fertilization].
abstract: OBJECTIVE
  To find out if the shortened exposure of oocytes to sperm can influence the embryo quality and the implantation and pregnancy rate of the classical IVF also in Centre for Assisted Reproduction.
  
  
  METHODS
  A prospective study including 104 patients undergoing the classical IVF procedure.
  
  
  METHODS
  Centre for Assisted Reproduction, Clinic of Gynaecology and Obstetrics, Palacký University Medical School and the University Hospital in Olomouc.
  
  
  METHODS
  In the study group with the shortened exposure (54 patients) the oocytes were washed one hour after the insemination and transferred into the fresh medium. In the control group (50 patients) the classical procedure (e.g. transfer into the fresh medium 17 to 20 hours after insemination) has been used. The following parameters have been evaluated in both groups: the stimulation protocol, sterility cause, number of previous IVF cycles, age, serum estradiol level on the day of hCG application, number of retrieved oocytes, fertility rate (FR), embryo quality, pregnancy rate (PR) and the implantation rate (IR) expressed as the number of gestational sacs with heart action related to the number of transferred embryos. T-Test, Mann-Whitney Test and chi 2 were used for statistical evaluation.
  
  
  RESULTS
  There were no statistically significant differences between the study and the control group in any of the parameters compared.
  
  
  CONCLUSIONS
  In our study group the shortened exposure of oocytes to sperm did not influence the results of the classical IVF.
authors.affiliation: Centrum asistované reprodukce, Gynekologicko-porodnická klinika FN a LF UP, Olomouc.
authors.forename: J
authors.initials: J
authors.lastname: Brezinová
authors.forename: M
authors.initials: M
authors.lastname: Labanová
authors.forename: J
authors.initials: J
authors.lastname: Dostál
authors.forename: I
authors.initials: I
authors.lastname: Oborná
authors.forename: S
authors.initials: S
authors.lastname: Machac
authors.forename: M
authors.initials: M
authors.lastname: Kudela
mesh_terms: D000328:Adult; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D011247:Pregnancy; D011446:Prospective Studies; D013084:Sperm-Ovum Interactions; D013997:Time Factors