The nucleus together with the cytosol generates patterns of specific cellular calcium signatures in tobacco suspension culture cells.
Plant cell suspension cultures respond to osmotic changes by alterations in levels of free cellular calcium. Using the aequorin recombinant method, we have measured the spatial and temporal characteristics of calcium signatures in the nucleus and the cytosol of BY-2 tobacco suspension cells challenged with hypo- or hyper-osmotic shock. We show here that the nuclear compartment contributes together with the cytosol to produce calcium signal patterns that discriminate hypo- from hyper-osmotic treatments, i.e. turgor from tension. We also demonstrate that calcium responses in the nucleus and the cytosol are differentially modulated by the strength and the nature of hyper-osmotic treatments. We conclude that qualitative and quantitative changes in the parameters of an external stimulus such as osmotic changes are converted into calcium signatures, distinctive in their temporal and subcellular characteristics, involving both the nucleus and the cytosol. Our results illustrate the versatility of calcium signaling in plant cells. In addition to the physiological 'address' of the cell, the compartmentation of the calcium signal is probably an important parameter in encoding response specificity.
Pauly
N
N
Signaux et Messages Cellulaires chez les Végétaux, Centre National de la Recherche Scientifique, Université Paul Sabatier, Castanet-Tolosan, France.
Knight
M R
MR
Thuleau
P
P
Graziana
A
A
Muto
S
S
Ranjeva
R
R
Mazars
C
C
D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D005682:Gadolinium / Q000494:pharmacology*; D009997:Osmotic Pressure; D014026:Nicotiana / Q000166:cytology
D002121:Calcium Channel Blockers; D005682:Gadolinium