title: Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein.
abstract: Recombinant Escherichia coli cells engineered for the expression of the xylB gene in conjunction with genes of the nonmevalonate pathway were supplied with (13)C-labeled 1-deoxy-D-xylulose. Cell extracts were analyzed directly by NMR spectroscopy. (13)C-labeled 2C-methyl-D-erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xylB, ispC, ispD, ispE, and ispF. The additional expression of the gcpE gene afforded 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate as an intermediate of the nonmevalonate pathway. Hypothetical mechanisms involving conserved cysteine residues are proposed for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate catalyzed by the GcpE protein.
authors.affiliation: Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hecht
authors.forename: W
authors.initials: W
authors.lastname: Eisenreich
authors.forename: P
authors.initials: P
authors.lastname: Adam
authors.forename: S
authors.initials: S
authors.lastname: Amslinger
authors.forename: K
authors.initials: K
authors.lastname: Kis
authors.forename: A
authors.initials: A
authors.lastname: Bacher
authors.forename: D
authors.initials: D
authors.lastname: Arigoni
authors.forename: F
authors.initials: F
authors.lastname: Rohdich
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D004798:Enzymes; D008798:Mevalonic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D017386:Sequence Homology, Amino Acid; D013729:Terpenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004798:Enzymes; D013729:Terpenes; C421242:hydroxymethylbutenyl 4-diphosphate synthase; D008798:Mevalonic Acid