Variations in the csgD promoter of Escherichia coli O157:H7 associated with increased virulence in mice and increased invasion of HEp-2 cells.
Promoter alterations in the csgD gene of Escherichia coli O157:H7 strains ATCC 43894 and ATCC 43895 are associated with variations in curli expression and the ability to bind Congo red dye. Red variants of each strain were more invasive for cultured HEp-2 cells than were white variants. An ATCC 43895 red variant was more virulent than a white variant in a mouse model. However, there were no differences in Shiga toxin production between red and white variants.
Uhlich
Gaylen A
GA
Roman L. Hruska U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933, USA. guhlich@arserrc.gov
Keen
James E
JE
Elder
Robert O
RO
D000818:Animals; D002522:Chlorocebus aethiops; D004396:Coloring Agents; D003224:Congo Red; D004195:Disease Models, Animal; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D014644:Genetic Variation; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic; D022621:Shiga Toxin / Q000096:biosynthesis; D015534:Trans-Activators / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014774:Virulence
D004396:Coloring Agents; C499694:CsgD protein, E coli; D029968:Escherichia coli Proteins; D005839:Gentamicins; D015534:Trans-Activators; D003224:Congo Red; D022621:Shiga Toxin