title: Drugs of abuse in a non-conventional sample; detection of methamphetamine and its main metabolite, amphetamine in abusers' clothes by HPLC with UV and fluorescence detection.
abstract: In this paper, we report the detection of methamphetamine and its major metabolite, amphetamine, in garments belong to known-abusers. These compounds were extracted from the textile using a mixture of chloroform:propan-2-ol (3:1, v/v), derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl) benzoyl chloride and separated using a reversed-phase high-performance liquid chromatography. The derivatives were detected by measuring either fluorescence at 440 nm or absorbance at 330 nm. By using 1-methyl-3-phenyl propylamine as an internal standard, calibration curves of spiked textile samples were linear over a wide range with correlation coefficients of 0.997 or better. Detection limits at a signal-to-noise ratio of 3 were less than or equal to 37.3 and 0.4 pg on column for the high-performance liquid chromatography-ultraviolet and -fluorescence detection methods, respectively. Intra- and inter-day variations at high and low concentrations (n > or = 3) were < or =12.7%. The developed methods were successfully applied to the determination of methamphetamine and amphetamine in clothes samples belong to abusers.
authors.affiliation: Department of Analytical Research for Pharmacoinformatics, Graduate School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.
authors.forename: O Y
authors.initials: OY
authors.lastname: Al-Dirbashi
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Kuroda
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000433:1-Propanol; D000661:Amphetamine / Q000032:analysis / Q000652:urine; D000662:Amphetamines / Q000032:analysis* / Q000652:urine; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003020:Clothing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013287:Illicit Drugs / Q000032:analysis*; D008694:Methamphetamine / Q000032:analysis / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*; D013542:Sweat / Q000737:chemistry*
chemical_list: D000662:Amphetamines; D013287:Illicit Drugs; D008694:Methamphetamine; D002725:Chloroform; D000433:1-Propanol; D000661:Amphetamine