Pergolide protects SH-SY5Y cells against neurodegeneration induced by H(2)O(2).
We found that pergolide, a dopamine D1/D2 receptor agonist used in the clinical therapy of Parkinson's disease, protects SH-SY5Y neuroblastoma cells from cell death induced by a brief pulse (15 min) of 1 mM H(2)O(2). Neuroprotection was found when pergolide was added to the culture medium either simultaneously with (EC(50)=60 nM) or 2 h before (EC(50)=40 nM) H(2)O(2) treatment. These effects were not blocked by different dopamine receptor antagonists. Our data suggest that pergolide, independently of dopamine receptor stimulation, may interfere with the early phases of the oxidative stress-induced neurotoxic process.
Uberti
Daniela
D
Deparment of Biomedical Sciences and Biotechnologies, School of Medicine, University of Brescia, Via Valsabbina 19, 25123, Brescia, Italy.
Piccioni
Laura
L
Colzi
Anna
A
Bravi
Daniele
D
Canonico
Pier Luigi
PL
Memo
Maurizio
M
D017209:Apoptosis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D009447:Neuroblastoma / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D010479:Pergolide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D018696:Neuroprotective Agents; D010479:Pergolide; D006861:Hydrogen Peroxide