Functional and compositional studies of arteries stored in University of Wisconsin solution compared with Krebs-Bülbring buffer.
Endothelium-dependent and -independent vasorelaxation in ring segments of rabbit thoracic aorta is reduced and noradrenaline-induced vasoconstriction unaltered after prolonged storage in University of Wisconsin solution (UW) compared to arteries stored in extracellular-type solutions such as Krebs-Bülbring buffer (KBB). The aims of the present study were to determine whether angiotensin-II-induced vasoconstriction, alterations in myosin light chains, protein synthetic capacity, and subcellular structures are altered after 8 days of UW storage at 4 degrees C. The present study showed reduced contractility to angiotensin II, following 8 days of cold storage in UW, that was not reversed in the presence of a nitric oxide synthase inhibitor, N(G)-nitro-l-arginine methyl ester (100 microM). Measurements of contractile protein ratios in the same tissues after cold storage in UW or KBB did not show any significant alterations in smooth muscle myosin light chains or protein synthetic capacity (reflected by total RNA). It is concluded that reductions in vasoconstriction in UW-stored tissue are unlikely to be due to increased release of nitric oxide nor reduced availability of myosin light chains for phosphorylation and vasoconstriction.
Alexander
B
B
Department of Surgery, GKT School of Medicine and Dentistry, London, UK.
Gryf-Lowczowski
J V
JV
Marway
J S
JS
Salisbury
J
J
Sherlock
D
D
Preedy
V
V
Benjamin
I S
IS
D000804:Angiotensin II; D000818:Animals; D001013:Aorta, Thoracic; D001158:Arteries / Q000033:anatomy & histology / Q000737:chemistry* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007537:Isometric Contraction; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology / Q000737:chemistry / Q000502:physiology; D018994:Myosin Light Chains / Q000032:analysis; D019188:Organ Preservation Solutions; D011817:Rabbits; D013997:Time Factors; D014021:Tissue Preservation; D014661:Vasoconstriction
D019394:Ki-67 Antigen; D018994:Myosin Light Chains; D019188:Organ Preservation Solutions; D000804:Angiotensin II