title: Phytoecdysteroids from the juice of Serratula coronata L. (Asteraceae).
abstract: Seven phytoecdysteroids have been isolated from Serratula coronata L. One of them is a new phytoecdysteroid, 3-epi-20-hydroxyecdysone. Two further ecdysteroids, 20-hydroxyecdysone 22-acetate and taxisterone, are isolated from this species for the first time in addition to the typical S. coronata ecdysteroids, 20-hydroxyecdysone, ecdysone, ajugasterone C and polypodine B. The juice squeezed from aerial parts of fresh plants of S. coronata was extracted with ethyl acetate. The ecdysteroids were isolated by a combination of chromatographic techniques (mainly HPLC) and identified by 1D and 2D (1)H and (13)C NMR experiments and mass-spectrometry. The biological activities of 3-epi-20-hydroxyecdysone (EC(50)=1.6 x 10(-7) M), taxisterone (EC(50)=9.5 x 10(-8) M) and ajugasterone C (EC(50)=6.2 x 10(-8) M) have been determined in the Drosophila melanogaster B(II) bioassay for ecdysteroid agonist activity.
authors.affiliation: Institute of Petrochemistry and Catalysis, Bashkortostan Republic Academy of Sciences and the Ufa Scientific Centre of the Russian Academy of Sciences, 450075, Ufa, prosp. Oktyabrya 141, Russia.
authors.forename: V N
authors.initials: VN
authors.lastname: Odinokov
authors.forename: I V
authors.initials: IV
authors.lastname: Galyautdinov
authors.forename: D V
authors.initials: DV
authors.lastname: Nedopekin
authors.forename: L M
authors.initials: LM
authors.lastname: Khalilov
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: V V
authors.initials: VV
authors.lastname: Kachala
authors.forename: L
authors.initials: L
authors.lastname: Dinan
authors.forename: R
authors.initials: R
authors.lastname: Lafont
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D004441:Ecdysterone / Q000031:analogs & derivatives / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D013055:Spectrophotometry, Infrared / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D010936:Plant Extracts; C449037:ajugasterone C; C449036:taxisterone; D004441:Ecdysterone