Enhancement of tumorigenic, metastatic and in vitro invasive capacity of rat mammary tumor cells by transforming growth factor-beta.
We investigated the effects of transforming growth factor-beta (TGF-beta) on biological behavior of a weakly malignant rat mammary carcinoma ER-1 cell line. TGF-beta enhanced the tumorigenic and metastatic capacity of ER-1 cells and their in vitro invasiveness to rat mesothelial and endothelial cell. Further cell biological analysis indicated that the increased invasive and metastatic capacity of ER-1 cells by TGF-beta was due to the increase in cell motility and adhesion to the mesothelial and endothelial cell monolayers. Thus, it is suggested that TGF-beta acts on ER-1 cells as a progression-enhancing factor which stimulates their adhesive and motile activities.
Nakata
Daichi
D
Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, 060-0815, Sapporo, Japan.
Hamada
Jun ichi
Ji
Ba
Yi
Y
Matsushita
Kazuhiro
K
Shibata
Toshiyuki
T
Hosokawa
Masuo
M
Moriuchi
Tetsuya
T
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D004268:DNA-Binding Proteins; D004305:Dose-Response Relationship, Drug; D005260:Female; D018960:Hyaluronan Receptors / Q000276:immunology; D006820:Hyaluronic Acid / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D017874:Immediate-Early Proteins / Q000235:genetics; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000473:pathology*; D009687:Nuclear Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D029867:Xenopus Proteins
D000906:Antibodies; D015815:Cell Adhesion Molecules; D004268:DNA-Binding Proteins; D018960:Hyaluronan Receptors; D017874:Immediate-Early Proteins; C108668:MIER1 protein, Xenopus; D009687:Nuclear Proteins; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase