Concentrated autologous plasma protein: a biochemically neutral solder for tissue welding.
OBJECTIVE
Xenographic or allographic serum protein solders used for laser welding may have immunologic and/or pathogenic complications. The objective of these studies was to develop a safe, autologous solder.


METHODS
Five methods of preparing concentrated autologous plasma protein solder (CAPPS) were evaluated. Next, the CAPPS was evaluated via (1) thermal denaturation studies using differential scanning calorimetry, (2) tissue welding studies to characterize both acute and healing properties.


RESULTS
The optimal concentration method to produce CAPPS rapidly was a dialysis method using chemical (osmotic) forces. The CAPPS showed similar denaturation profiles to serum albumin (SA) solders. Acutely, CAPPS provided comparable breaking strengths to SA solders. At 7 days, there was no significant difference in breaking strength or histology between 50% human SA solder and CAPPS (using a porcine skin model).


CONCLUSIONS
These studies demonstrate that the CAPPS system provides acceptable acute and chronic properties for laser welding.
Stewart
R B
RB
ABIOMED, Inc., 22 Cherry Hill Drive, Danvers, MA 01923, USA. rstewart@abiomed.com
Bleustein
C B
CB
Petratos
P B
PB
Chin
K C
KC
Poppas
D P
DP
Kung
R T
RT
D000818:Animals; D001798:Blood Proteins / Q000138:chemical synthesis / Q000627:therapeutic use*; D002152:Calorimetry, Differential Scanning; D004195:Disease Models, Animal; D004285:Dogs; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D053685:Laser Therapy / Q000379:methods*; D013536:Suture Techniques; D013552:Swine; D013718:Tensile Strength / Q000502:physiology; D014014:Tissue Adhesives / Q000138:chemical synthesis / Q000627:therapeutic use*; D014182:Transplantation, Autologous; D014896:Welding / Q000379:methods*; D014945:Wound Healing / Q000502:physiology
D001798:Blood Proteins; D014014:Tissue Adhesives