title: Effect of cholesteryl side chain and complexing with cholic acid on gene transfection by cationic poly(ethylene glycol) derivatives.
abstract: A new class of poly(ethylene glycol) derivative, Chol-PEG-A, having both cholesteryl- and amino-pendant groups (2.89 and 5.39 groups per polymer molecule, respectively) was synthesized. This amphiphilic PEG derivative forms a cationic polymer assembly in water. Chol-PEG-A expressed high transfection efficiency in the serum-free medium at relatively low amine/phosphate (N/P) ratios (1.3-1.5), whereas PEG-A, having only amino pendants without the cholesteryl side chain could transfect the cells only at a very high N/P ratio (83). The efficiency remarkably decreased by addition of fetal bovine serum (FBS) to the medium. Mixing of cholic acid to Chol-PEG-A gave a neutralized polyion complex, and the interaction with serum proteins was evidently suppressed. This Chol-PEG-A-cholic acid system showed higher gene expression even in the FBS-containing medium and as high a transfection efficiency as a Superfect.
authors.affiliation: Department of Home Economics, Otsuma Women's University, 12 Sanban-cho, Chiyoda-ku, 102-8357, Tokyo, Japan. koyama@otsuma.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D019556:COS Cells; D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005079:Excipients / Q000138:chemical synthesis / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D002412:Cations; D005079:Excipients; D011092:Polyethylene Glycols; D002784:Cholesterol