[Molecular studies in a population of children with bronchial asthma. I. Polymorphism in the promotor region of gene CD14].
Allergic asthma is associated with the recruitment of the inflammatory cells into the bronchial mucosa. Surface expression of CD14, a marker of activation and differentiation of macrophages/monocytes, was suggested to protect against Th-2 response.


OBJECTIVE
To investigate clinical effects of genetic polymorphism of CD14 promoter in asthmatic children.


METHODS
In ISAAC survey, 50 children with asthma were identified (wheezing in the last year, serum IgE level > 150 kIU/l, positive bronchial challenge test with aerolized hypertonic saline) and 73 children without the above signs. Age range of surveyed children was 13-14 years. Genotypic pattern of CD14 promoter -159 C to T transition was assessed by RFLP method.


RESULTS
There was no difference in the allelic (0.36 vs. 0.38 for -159T) or genotype frequencies (0.12 vs. 0.15 for -159TT) of CD14 polymorphism between allergic asthmatics and controls. Moreover, there was no relationship between CD14 genotype and serum IgE level or bronchial hyper-responsiveness.


CONCLUSIONS
Our results do not confirm the association of CD14 polymorphism (promoter -159 C to T transition) with asthma in Polish children.
Lis
G
G
Kliniki Chorób Dzieci, Collegium Medicum UJ, Kraków.
Kostyk
E
E
Sanak
M
M
Pietrzyk
J J
JJ
D000293:Adolescent; D001249:Asthma / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D001980:Bronchi / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics* / Q000276:immunology; D008297:Male; D020556:Neutrophil Infiltration; D011044:Poland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length
D018950:Lipopolysaccharide Receptors