Expression of 5-HT2A receptor mRNA in rat nucleus raphe magnus neurons after peripheral inflammation.
OBJECTIVE
Examine the expression of 5-HT2A receptor mRNA and co-localization of 5-HT2A receptor mRNA with 5-HT immunoreactivity in nucleus raphe magnus (NRM) neurons following carrageenan inflammation.


METHODS
In situ hybridization (ISH) and combining fluorescent ISH with immunofluorescent histochemical double staining technique were used in the present study.


RESULTS
5-HT2A receptor mRNA was expressed in moderate level in NRM neurons. Following carrageenan inflammation, the expression of 5-HT2A receptor mRNA in NRM was greatly increased. The peak occurred at 3 - 8 h and then there was a clear decrease at 24 h after injection of carrageenan. Only a few 5-HT2A receptor mRNA and 5-HT immunoreactive double labeled cells were observed in NRM, and the number of the double labeled cells had no obvious change following carrageenan inflammation.


CONCLUSIONS
The synthesis of 5-HT2A receptor was increased in non-serotonin rather than serotonin neurons of NRM during peripheral inflammation.
Zhang
Y Q
YQ
Institute of Neurobiology, Fudan University, Shanghai 200433, China.
Gao
X
X
Ji
G C
GC
Huang
Y L
YL
Wu
G C
GC
D000818:Animals; D002351:Carrageenan; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism; D009619:Nociceptors / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011903:Raphe Nuclei / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin; D002351:Carrageenan