Identification of a tyrosine kinase-phosphorylated protein in arachidonic acid- and prostaglandin A(2)-treated cells in vitro.
The effects of 20 microg/ml exogenous arachidonic acid (AA) and prostaglandin A(2) (PGA(2)) were evaluated on total tyrosine kinase (TK) activity and tyrosine phosphorylation status in HeLa and MCF-7 cells. AA and PGA(2) increased TK activity in both HeLa and MCF-7 cells. Western blotting employing an anti-phosphotyrosine antibody showed only one protein of approximately 55 kDa (approximately 55 kDa) to be phosphorylated in the MCF-7 cells, while a variety of proteins were phosphorylated in the HeLa cells, including the approximately 55 kDa protein. Amino acid analyses as well as Matrix Assisted Laser Desorption Ionization were conducted on this protein from different cell lines and it was shown to be similar. Comparison to p53 did not show similarities. The identity of this protein needs to be further characterized to help elucidate the signal transduction pathways of AA and PGA(2).
Joubert
A M
AM
Department of Physiology, University of Pretoria, Pretoria, 0001, South Africa. ajoubert@postillion.up.ac.za
Panzer
A
A
Joubert
F
F
Lottering
M L
ML
Bianchi
P C
PC
du Toit
P J
PJ
Seegers
J C
JC
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D010750:Phosphoproteins / Q000032:analysis*; D010766:Phosphorylation; D011454:Prostaglandins A / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D010750:Phosphoproteins; D011454:Prostaglandins A; D016718:Arachidonic Acid; D011505:Protein-Tyrosine Kinases; C100008:prostaglandin A2