title: Allelic variation of BAT-26 and BAT-40 poly-adenine repeat loci in North Indians.
abstract: Analysis of mononucleotide repeats BAT-26 and BAT-40 in North Indians revealed that there were germline polymorphisms at both the loci. We evaluated BAT-26 and BAT-40 in 100 normal healthy individuals from North India. The DNA from normal blood was PCR amplified using primers flanking the BAT-26 and BAT-40 loci. The allelic variation of BAT-26 and BAT-40 ranged between 117-130 and 94-112 bp respectively. The most frequent BAT-26 allele was 122 bp, which corresponded to 26 repeats and had a frequency of 32% while that of BAT-40 was 109 bp corresponding to 39 repeats with a frequency of 26%. These results suggest that polymorphisms in these poly-adenine repeat loci limit their applicability in studying the microsatellite instability in cancers.
authors.affiliation: Department of Medical Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, India.
authors.forename: Monisha
authors.initials: M
authors.lastname: Mukherjee
authors.forename: Vaish
authors.initials: V
authors.lastname: Minal
authors.forename: R D
authors.initials: RD
authors.lastname: Mittal
authors.forename: Balraj
authors.initials: B
authors.lastname: Mittal
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000483:Alleles; D004268:DNA-Binding Proteins; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D006579:Heterozygote; D006801:Humans; D007194:India; D018895:Microsatellite Repeats; D051718:MutS Homolog 2 Protein; D011061:Poly A / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D011061:Poly A; D015096:3-Hydroxysteroid Dehydrogenases; C497172:MSH2 protein, human; D051718:MutS Homolog 2 Protein