A novel progestogen receptor subtype in the Japanese eel, Anguilla japonica.
A cDNA encoding a second type of a progestogen receptor (ePR2) was isolated from the same library as we had previously cloned a functional PR (ePR1) in eel testis. The amino acid sequence of the ePR2 shows low homology with ePR1 (34%), but both PRs showed progestogen-dependent transactivation in transfection experiment. Tissue distribution of ePR2 mRNA was clearly different from that of ePR1. Protein interaction between two PRs was demonstrated in vitro by a glutathione S-transferase pull-down assay. These results indicate that ePR2 is also a functional PR. This is the first isolation of two different functional PR molecules from a vertebrate.
Ikeuchi
Toshitaka
T
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Todo
Takashi
T
Kobayashi
Tohru
T
Nagahama
Yoshitaka
Y
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D004524:Eels; D005260:Female; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006908:Hydroxyprogesterones / Q000378:metabolism / Q000494:pharmacology; D007564:Japan; D008024:Ligands; D008297:Male; D008969:Molecular Sequence Data; D011372:Progestins / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D015533:Transcriptional Activation
D018076:DNA, Complementary; D006908:Hydroxyprogesterones; D008024:Ligands; D011372:Progestins; D020033:Protein Isoforms; D011987:Receptors, Steroid; C444959:progestogen receptor 2, Anguilla japonica; C007433:17,20-dihydroxy-4-pregnen-3-one; D005982:Glutathione Transferase