A catalytic subunit of the sugar beet protein kinase CK2 is induced by salt stress and increases NaCl tolerance in Saccharomyces cerevisiae.
Salinity is an important limiting factor in plant growth and development. We have cloned a catalytic subunit of the sugar beet protein kinase CK2 (BvCKA2) by functional expression in yeast of a NaCl-induced cDNA library. BvCKA2 was able to increase the yeast tolerance to NaCl and to functionally complement the cka1 cka2 yeast double mutant upon over-expression. Southern blot analysis indicated that, in sugar beet, the BCKA2 gene is a member of a multigene family. The mRNA levels of BvCKA2 were up-regulated in response to NaCl stress which suggests that protein kinase CK2 may be involved in the plant response to salt stress.
Kanhonou
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Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
Serrano
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Palau
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RR
D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D027461:Beta vulgaris / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D047390:Casein Kinase II; D020134:Catalytic Domain; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D011188:Potassium / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
D018076:DNA, Complementary; D018744:DNA, Plant; D012965:Sodium Chloride; D012964:Sodium; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D011188:Potassium