Capillary zone electrophoresis of coniferyl alcohol oxidation products.
Capillary zone electrophoresis (CZE) was developed for the quantitative determination of dimers obtained by horseradish peroxidase-catalyzed oxidation of coniferyl alcohol. The influence of pH, electrolyte concentration, applied voltage, and temperature on CZE performance was investigated, resulting in an efficient and rapid separation. Coniferyl alcohol-derived dimers were directly analyzed from their reaction mixtures, without any extraction or derivatization step. In addition, these dimers were analyzed within 14 min, a substantially shorter time than is required for the HPLC method or the conventional capillary gas chromatography of their silylated derivatives. Standard deviations between injection replicates were in the 0.4-0.7% range for migration times and in the 1.8-5.1% range for relative normalized peak areas. The method could therefore be successfully applied to follow the peroxidase-catalyzed oxidation of coniferyl alcohol.
Fournand
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D
Laboratoire de Chimie Biologique, INRA-INAPG, F-78850 Thiverval-Grignon, France. fournand@grignon.inra.fr
Lapierre
C
C
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002934:Cinnamates / Q000737:chemistry*; D019281:Dimerization; D019075:Electrophoresis, Capillary / Q000379:methods; D006735:Horseradish Peroxidase; D007700:Kinetics; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
D002934:Cinnamates; D006735:Horseradish Peroxidase; C016316:coniferin