Male partner screening before in vitro fertilization: preselecting patients who require intracytoplasmic sperm injection with the sperm penetration assay.
OBJECTIVE
To determine the diagnostic accuracy of the sperm penetration assay (SPA) and standard semen parameters for subsequent fertilization in in vitro fertilization-embryo transfer (IVF-ET).


METHODS
Prospective study.


METHODS
Andrology Laboratory, and university research laboratory.


METHODS
Two hundred sixteen couples undergoing male-partner screening before IVF-ET (265 cycles).


METHODS
Male-partner screening (semen analyses [SA] and SPA), standard IVF-ET procedures, follow-up of fertilization in IVF-ET.


METHODS
Diagnostic accuracy of SA and SPA for prediction of fertilization in IVF-ET.


RESULTS
The SPA predicted IVF fertilization with high negative (84%) and positive (98%) predictive rates, and correct prediction in 88% of cycles. In contrast, sperm concentration, motility, morphology, and complete SA showed poor diagnostic accuracy, with correct prediction of IVF fertilization in 64%, 65%, 45%, and 68% of cycles, respectively.


CONCLUSIONS
Very low sperm concentration and/or motility were good predictors of poor IVF fertilization, however, low to normal semen parameters were not predictive of successful IVF fertilization. The SPA is a useful screening tool that predicts IVF fertilization with high diagnostic accuracy. The SPA may be useful to discriminate between those couples with a high probability of normal fertilization in IVF and those with a low probability of normal fertilization that may benefit from assisted fertilization by intracytoplasmic sperm injection (ICSI).
Freeman
M R
MR
Nashville Fertility Center, Nashville, Tennessee 37203, USA. mfreeman@nashvillefertility.com
Archibong
A E
AE
Mrotek
J J
JJ
Whitworth
C M
CM
Weitzman
G A
GA
Hill
G A
GA
D000818:Animals; D006224:Cricetinae; D004624:Embryo Transfer; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005306:Fertilization / Q000502:physiology; D006801:Humans; D008297:Male; D008647:Mesocricetus; D011237:Predictive Value of Tests; D011247:Pregnancy; D011446:Prospective Studies; D012044:Regression Analysis; D012680:Sensitivity and Specificity; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions / Q000502:physiology*