Role of diacylglycerol in PKD recruitment to the TGN and protein transport to the plasma membrane.
Protein kinase D (PKD) is a cytosolic serine-threonine kinase that binds to the trans-Golgi network (TGN) and regulates the fission of transport carriers specifically destined to the cell surface. PKD was found to bind diacylglycerol (DAG), and this binding was necessary for its recruitment to the TGN. Reducing cellular levels of DAG inhibited PKD recruitment and blocked protein transport from the TGN to the cell surface. Thus, a DAG-dependent, PKD-mediated signaling regulates the formation of transport carriers from the TGN in mammalian cells.
Baron
Carole L
CL
Section of Cell and Developmental Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Malhotra
Vivek
V
D002264:Carboxylic Acids / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D037341:Fumonisins; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D050356:Lipid Metabolism; D008081:Liposomes / Q000378:metabolism; D008562:Membrane Glycoproteins; D010712:Phosphatidic Acids / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D011493:Protein Kinase C / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism; D015398:Signal Transduction; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014759:Viral Envelope Proteins / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
D002264:Carboxylic Acids; D004075:Diglycerides; D004791:Enzyme Inhibitors; D037341:Fumonisins; C028391:G protein, vesicular stomatitis virus; D008081:Liposomes; D008562:Membrane Glycoproteins; D010712:Phosphatidic Acids; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; C044295:1,2-dioctanoylglycerol; C056933:fumonisin B1; D011433:Propranolol; C089212:protein kinase D; D011493:Protein Kinase C