title: Historical control data on urinary and renal tissue biomarkers in naive male Wistar rats.
abstract: Total proteins, angiotensin-converting enzyme, N-acetyl-beta-D-glucosaminidase, glutamine transaminase K and glutamine synthetase were determined in urine collected overnight (14 h: 6:00 p.m.-8:00 a.m.) from naive male Wistar rats; glutamine transaminase K and glutamine synthetase in the kidney 10,000 g supernatant and p-aminohippurate uptake in renal cortical slices also were measured. Urinary parameters were related both to urinary creatinine concentration and urinary flow rate; kidney parameters were related to protein concentration (enzymes) or slice/medium (S/M) ratio (p-aminohippurate uptake). The following reference ranges (1.0 and 99.0 percentiles) were obtained: urine: total urinary proteins (195 samples) 0.03-0.29 g mmol(-1) creatinine and 0.13-1.77 mg h(-1); angiotensin-converting enzyme (115 samples) 8.9-63.7 micromol mmol(-1) creatinine and 59.4-282.7 nmol h(-1); glutamine transaminase K (115 samples) 0-1.7 micromol mmol(-1) creatinine and 0-8.5 nmol h(-1); N-acetyl-beta-D-glucosaminidase (72 samples) 0.7-5.0 micromol mmol(-1) creatinine and 4.9-28.4 nmol h(-1) (naive male rats did not excrete glutamine synthetase); kidney: glutamine transaminase K (36 samples) 14.5-32.8 nmol mg(-1) protein; glutamine synthetase (22 samples) 13.9-48.6 nmol mg(-1) protein and p-aminohippurate (54 samples) 4.77-17.89 S/M. Urinary creatinine (r = -0.780), total urinary proteins (r = -0.521), angiotensin-converting enzyme (r = -0.650) and N-acetyl-beta-D-glucosaminidase (r = -0.796) but not glutamine transaminase K were well correlated with diuresis. In addition, the same parameters, but not glutamine transaminase K, were well correlated with creatinine (r = 0.604,0.701 and 0.747, respectively). Significant correlation also was observed between urinary indices adjusted to creatinine or urinary flow rate (total urinary proteins: r = 0.813; angiotensin-converting enzyme: r = 0.677; glutamine transaminase K: r = 0.939; N-acetyl-beta-D-glucosaminidase: r = 0.657). Finally, a low but significant correlation was found between total urinary proteins and angiotensin-converting enzyme (r = 0.293) and N-acetyl-beta-D-glucosaminidase (r = 0.471).
authors.affiliation: Department of Environmental Medicine and Public Health, University of Padova, Padova, Italy. andres.trevisan@unipd.it
authors.forename: A
authors.initials: A
authors.lastname: Trevisan
authors.forename: M
authors.initials: M
authors.lastname: Giraldo
authors.forename: M
authors.initials: M
authors.lastname: Borella
authors.forename: S
authors.initials: S
authors.lastname: Maso
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D003404:Creatinine / Q000652:urine; D005974:Glutamate-Ammonia Ligase / Q000652:urine; D007672:Kidney Cortex / Q000378:metabolism; D008190:Lyases / Q000652:urine; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000652:urine; D011507:Proteinuria; D051381:Rats; D017208:Rats, Wistar / Q000502:physiology*; D012016:Reference Values; D000637:Transaminases / Q000652:urine; D016482:Urinalysis; D014554:Urination; D014563:Urodynamics; D010130:p-Aminohippuric Acid / Q000378:metabolism
chemical_list: D015415:Biomarkers; D003404:Creatinine; D000637:Transaminases; C427605:glutamine - phenylpyruvate transaminase; D000118:Acetylglucosaminidase; D007703:Peptidyl-Dipeptidase A; D008190:Lyases; D005974:Glutamate-Ammonia Ligase; D010130:p-Aminohippuric Acid