title: Calcineurin-dependent mitochondrial disturbances in calcium-induced apoptosis of human immunodeficiency virus gp160-expressing CD4+ cells.
abstract: In CD4+ UE160 cells with inducible expression of gp160, mechanisms of apoptosis induced by human immunodeficiency virus (HIV) Env protein were analyzed. Induction of gp160 caused intracellular calcium increase followed by the release of cytochrome c from mitochondria, which was inhibited by calcineurin inhibitors. Association of BAD with Bcl-xL was observed, and a portion of BAD was dephosphorylated after induction of gp160. These data suggested that calcineurin plays a role in the HIV Env-induced apoptosis in a mitochondrion-dependent way.
authors.affiliation: Department of Virology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Sasaki
authors.forename: Kozo
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Koga
authors.forename: Genki
authors.initials: G
authors.lastname: Kimura
authors.forename: Kikuo
authors.initials: K
authors.lastname: Nomoto
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yoshida
mesh_terms: D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019256:Cadmium Chloride / Q000494:pharmacology; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D019302:HIV Envelope Protein gp160 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014162:Transfection; D020298:U937 Cells
chemical_list: D019302:HIV Envelope Protein gp160; D019703:Calcineurin; D019256:Cadmium Chloride; D002118:Calcium