title: Genomic structure of proton-coupled oligopeptide transporter hPEPT1 and pH-sensing regulatory splice variant.
abstract: Proton-coupled oligopeptide transporter PEPT1 facilitates the transport of dipeptides and peptoid drugs (including antibiotics) across the cell membranes of endothelial and epithelial cells. Substrate transport by the proton symport is driven by pH gradients, while the profile of pH sensitivity is regulated by a closely related protein, hPEPT1-RF. We investigated the genomic structure of hPEPT1 and hPEPT1-RF. Analysis of the high-throughput genomic sequence (HTGS) database revealed that hPEPT1 and hPEPT1-RF are splice variants encoded by the same gene located in chromosome 13, consisting of 24 exons. hPEPT1 is encoded by 23 exons and hPEPT1-RF by 6 exons. Coding sequences of hPEPT1-RF share 3 exons completely and 2 exons partially with hPEPT1. The genomic organization of hPEPT1 shows high similarity with its mouse orthologue. Exon-intron boundaries occur mostly in the loops connecting transmembrane segments (TMSs), suggesting a modular gene structure reflecting the TMS-loop repeat units in hPEPT1. The putative promoter region of hPEPT1 contains TATA boxes and GC-rich regions and a potential insulin responsive element.
authors.affiliation: Department of Biopharmaceutical Sciences, University of California San Francisco, CA 94143-0446, USA. Arto.Urtti@messi.uku.fi
authors.forename: A
authors.initials: A
authors.lastname: Urtti
authors.forename: S J
authors.initials: SJ
authors.lastname: Johns
authors.forename: W
authors.initials: W
authors.lastname: Sadée
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008969:Molecular Sequence Data; D000074011:Peptide Transporter 1; D034444:Peptoids; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D000074011:Peptide Transporter 1; D034444:Peptoids; D020033:Protein Isoforms; C082186:SLC15A1 protein, human; C489941:Slc15a1 protein, mouse; D027981:Symporters