title: Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain.
abstract: Mutations in the human Crumbs homologue 1 (CRB1) gene cause severe retinal dystrophies. CRB1 is homologous to Drosophila Crumbs, a protein essential for establishing and maintaining epithelial polarity. We have isolated the mouse orthologue, Crb1, and analyzed its expression pattern in embryonic and post-natal stages. Crb1 is expressed exclusively in the eye, and the central nervous system. In the developing eye, expression of Crb1 is detected in the retinal progenitors, and later on becomes restricted to the differentiated photoreceptor cells where it remains active up to the adult stage. In the developing neural tube, expression of Crb1 is restricted to its most ventral structures, coinciding with the expression domain of Nkx2.2. In the adult brain, Crb1 expression is defined to areas where the production and migration of neurons occurs in adulthood.
authors.affiliation: Department of Human Genetics, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. a.denhollander@antrg.azn.nl
authors.forename: Anneke I
authors.initials: AI
authors.lastname: den Hollander
authors.forename: Michela
authors.initials: M
authors.lastname: Ghiani
authors.forename: Yvette J M
authors.initials: YJ
authors.lastname: de Kok
authors.forename: Jan
authors.initials: J
authors.lastname: Wijnholds
authors.forename: Andrea
authors.initials: A
authors.lastname: Ballabio
authors.forename: Frans P M
authors.initials: FP
authors.lastname: Cremers
authors.forename: Vania
authors.initials: V
authors.lastname: Broccoli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology* / Q000254:growth & development; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D005123:Eye / Q000196:embryology* / Q000254:growth & development; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D006801:Humans; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
chemical_list: C448939:CRB1 protein, human; C453730:Crb1 protein, mouse; D029721:Drosophila Proteins; D005136:Eye Proteins; D000090322:Homeobox Protein Nkx-2.2; D018398:Homeodomain Proteins; D008565:Membrane Proteins; C000715631:NKX2-2 protein, human; D009419:Nerve Tissue Proteins; C000715627:Nkx2-2 protein, mouse; D009687:Nuclear Proteins; D014157:Transcription Factors; C064360:crb protein, Drosophila