Inhibitory effect on immunoglobulin E production in vivo and in vitro by Siegesbeckia glabrescens.
Elevated levels of immunoglobulin (Ig)E are associated with immediate-type allergic reactions. The effect of an aqueous extract of Siegesbeckia glabrescens (Compositae) whole plants (SGWP) on in vivo and in vitro IgE production was studied in mice. SGWP dose-dependently inhibited the active systemic anaphylaxis and serum IgE production induced by immunization with ovalbumin and Bordetella pertussis toxin absorbed to aluminium hydroxide gel. SGWP dose-dependently inhibited IL-4-dependent IgE production by lipopolysaccharide-stimulated murine whole spleen cells. In the case of U266 human IgE-bearing B cells, SGWP also showed an inhibitory effect on IgE production. These results suggest that SGWP has an anti-allergic activity by inhibiting IgE production from B cells.
Kim
H M
HM
Department of Oriental Pharmacy, College of Pharmacy, Center of Oriental Medicinal Science, Wonkwang University, Sukjang-Dong, 707, Kyung-Ju City, Kyungpook 780-714, South Korea. hmkim@wonkwang.ac.kr
Lee
J H
JH
Won
J H
JH
Park
E J
EJ
Chae
H J
HJ
Kim
H R
HR
Kim
C H
CH
Baek
S H
SH
D000707:Anaphylaxis / Q000188:drug therapy* / Q000209:etiology; D000818:Animals; D019659:Asteraceae; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis*; D051379:Mice; D008813:Mice, Inbred ICR; D010047:Ovalbumin; D037342:Pertussis Toxin; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use*; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D010566:Virulence Factors, Bordetella
D010936:Plant Extracts; D010566:Virulence Factors, Bordetella; D007073:Immunoglobulin E; D010047:Ovalbumin; D037342:Pertussis Toxin