Coxsackie B viruses that use human DAF as a receptor infect pig cells via pig CAR and do not use pig DAF.
Coxsackie B viruses (CVB) are enteroviruses belonging to the family Picornaviridae. Serotypes 1, 3 and 5 of CVB bind to the human membrane complement regulator decay-accelerating factor (DAF) and the coxsackievirus/adenovirus receptor (CAR), using either or both as receptors. These viruses are known to infect pig cell lines, but the receptor(s) involved has not been identified. We have recently characterized the pig homologue of DAF and here explore the interactions of human DAF-binding CVB with pig homologues of DAF and CAR. CVB infection of three pig cell lines resulted in cytolysis, which could be not be blocked by anti-pig DAF antibodies. CVB bound to CHO cells transfected with human DAF, but not pig DAF. Modification of pig DAF by incorporation of the fourth short consensus repeat of human DAF did not confer CVB-binding capacity. CVB did bind CHO cells expressing pig or human CAR, and pre-incubation of pig cells with anti-CAR antibody blocked CVB infection.
Spiller
O Brad
OB
Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, 3rd floor Tenovus Building, Heath Park, Cardiff CF14 4XX, UK1.
Goodfellow
Ian G
IG
Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK2.
Evans
David J
DJ
Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow G11 5JR, UK2.
Hinchliffe
Stewart J
SJ
Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, 3rd floor Tenovus Building, Heath Park, Cardiff CF14 4XX, UK1.
Morgan
B Paul
BP
Complement Biology Group, Department of Medical Biochemistry, University of Wales College of Medicine, 3rd floor Tenovus Building, Heath Park, Cardiff CF14 4XX, UK1.
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D029822:Enterovirus B, Human / Q000378:metabolism* / Q000502:physiology; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014162:Transfection
D000906:Antibodies; D018958:CD55 Antigens; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins