title: Bcl-2 and p53 expression in insular and in well-differentiated thyroid carcinomas with an insular pattern.
abstract: Expression of p53 and bcl-2 oncogenes was investigated in poorly differentiated, so-called insular carcinomas of the thyroid gland and also in the follicular and papillary carcinomas with an insular component. Hematoxylin-eosin sections of 217 thyroid carcinomas were reevaluated for insular carcinoma and also for thyroid carcinomas with an insular component. Immunohistochemical staining method was used for detecting p53 and bcl-2 expression on paraffin blocks of three pure insular, five follicular or papillary thyroid carcinomas with a major insular component (more than 50%) and six with a minor insular component (20-50%). Flow cytometry was also performed in these cases. None of the cases showed p53 immunoreactivity. Bcl-2 expression was observed in all cases and the most intense staining was seen in insular areas. All the cases were diploid. We suggest that bcl-2 plays a role in loss of differentiation of thyroid carcinomas.
authors.affiliation: Department of Pathology, Akdeniz University, Antalya, Turkey.
authors.forename: H E
authors.initials: HE
authors.lastname: Peştereli
authors.forename: M
authors.initials: M
authors.lastname: Oğüş
authors.forename: N
authors.initials: N
authors.lastname: Oren
authors.forename: G
authors.initials: G
authors.lastname: Karpuzoğlu
authors.forename: T
authors.initials: T
authors.lastname: Kerpuzoğlu
mesh_terms: D000328:Adult; D000368:Aged; D018265:Carcinoma, Papillary, Follicular / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53