title: Interaction of bone proteoglycans and proteoglycan components with hydroxyapatite.
abstract: The small leucine-rich proteoglycans (SLRPs) of bone interact with hydroxyapatite (HAP) and are proposed to play an important role in the regulation of the mineralisation process. The present study has examined the interaction of bone SLRPs, purified, liberated bone glycosaminoglycan (GAG) chains and core proteins, as well as commercial chondroitin 4-sulphate (C4S) with HAP. Isotherm data (0.02 M sodium acetate) revealed that the intact proteoglycans (PGs) and bone GAGs showed greater binding onto HAP with higher adsorption maxima than the constituent core proteins and commercial C4S. Adsorption was dependent on pH and ionic strength, increasing with decreasing pH and in the presence of calcium whilst decreasing in the presence of phosphate, suggesting that electrostatic effects are important. The data indicates that PG/GAG chemistry and conformation in solution are significant determinants in the adsorption process and provides important information concerning interfacial adsorption phenomena between the organic-inorganic phases of mineralised systems.
authors.affiliation: Department of Basic Dental Science, Dental School, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XY, Wales, UK. reessg1@cardiff.ac.uk
authors.forename: S G
authors.initials: SG
authors.lastname: Rees
authors.forename: D T
authors.initials: DT
authors.lastname: Hughes Wassell
authors.forename: R J
authors.initials: RJ
authors.lastname: Waddington
authors.forename: G
authors.initials: G
authors.lastname: Embery
mesh_terms: D000327:Adsorption; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002113:Calcification, Physiologic; D002118:Calcium / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007930:Leucine / Q000737:chemistry; D008968:Molecular Conformation; D009994:Osmolar Concentration; D010710:Phosphates / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification; D012756:Sheep
chemical_list: D006025:Glycosaminoglycans; D010710:Phosphates; D011509:Proteoglycans; D002809:Chondroitin Sulfates; D017886:Durapatite; D007930:Leucine; D002118:Calcium