title: Comparative phylogeny of the ammonia monooxygenase subunit A and 16S rRNA genes of ammonia-oxidizing bacteria.
abstract: A fragment of the ammonia monooxygenase gene (amoA) from 31 strains of ammonia-oxidizing bacteria (AOB) was sequenced and analysed phylogenetically. The results were compared with the phylogeny of 16S rDNA from AOB. For most groups of AOB we found a high consistency between the phylogenetic trees based on the 16S rDNA and amoA sequences. Although it is not a phylogenetic marker, using the amoA as a probe when studying microbial diversity will probably reduce the amount of non-AOB detected, compared to using rDNA based probes. The data presented in this paper extend and improve the basis for application of amoA in studies of AOB in the environment.
authors.affiliation: Laboratory of Microbial Gene Technology, Department of Chemistry and Biotechnology, Agricultural University of Norway, P.O. Box 5051, N-1432, As, Norway. agot.aakara@ikb.nlh.no
authors.forename: A
authors.initials: A
authors.lastname: Aakra
authors.forename: J B
authors.initials: JB
authors.lastname: Utåker
authors.forename: I F
authors.initials: IF
authors.lastname: Nes
mesh_terms: D005798:Genes, Bacterial; D010088:Oxidoreductases / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D012329:RNA, Bacterial / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000145:classification* / Q000235:genetics; D013045:Species Specificity
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C045582:ammonia monooxygenase