title: Strain differences in the toxicity of cadmium to trigeminal ganglia in mice.
abstract: Cadmium (Cd) is toxic to sensory ganglia in many animal species. Cadmium uptake is low in the central nervous system, but it distributes preferentially to peripheral sensory and autonomic ganglia. Strain differences have been demonstrated in the sensitivity of mice to Cd-induced hepatotoxicity, testicular toxicity, and teratogenicity. To study the sensitivity of different mouse strains to Cd toxicity in sensory ganglia, eight strains of mice (four sensitive to testicular toxicity: 129/SVIM, AKR/J, DBA/1J, and C57BR/J; and four resistant: Balb/C, C3H/HeJ, A/J, and C57BL/6J) were given 15 micromol CdCl(2)/kg iv. Trigeminal ganglia (TG) were harvested 24 h later and examined by light microscopy for pathologic lesions. Cadmium induced degeneration of ganglion cells in five strains, namely 129/SVIM, AKR/J, DBA/1J, C57BR/J, and C3H/HeJ mice. These are the same strains that show sensitivity to testicular toxicity, except for C3H/HeJ, which is resistant to testicular toxicity. Cd also induced focal hemorrhages around the ganglion cells and nerve fibers in two of these strains (129/SVIM and AKR/J) and scattered foci of necrosis in C3H/HeJ and 129/SVIM strains. There was no morphologic abnormality in three strains, namely Balb/C, A/J, and C57BL/6J. To examine the mechanism of these strain differences in toxicity, all eight strains of mice were given a nontoxic dose of Cd (0.4 micromol CdCl(2)/kg, 20 microCi (109)Cd/kg iv). Cadmium distribution to the brain and trigeminal ganglia was determined 30 min later by gamma scintillation spectrometry. Cadmium content in the brain was very low and did not differ among the eight strains. In contrast, Cd content was higher in trigeminal ganglia of four of the five strains showing trigeminal ganglia sensitivity than in the three strains showing resistance. In conclusion, the toxicity of Cd to trigeminal ganglia is different among various strains of mice. This strain difference in toxicity appears to be due, at least in part, to differences in the distribution of Cd to the ganglia, but it is clearly not the only factor.
authors.affiliation: Center for Environmental and Occupational Health, University of Kansas Medical Center, Kansas City, Kansas 66160-7417, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Habeebu
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: J D
authors.initials: JD
authors.lastname: Park
authors.forename: C D
authors.initials: CD
authors.lastname: Klaassen
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects; D001923:Brain Chemistry; D002104:Cadmium / Q000032:analysis / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D006470:Hemorrhage / Q000139:chemically induced / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009336:Necrosis; D019636:Neurodegenerative Diseases / Q000139:chemically induced* / Q000473:pathology; D013045:Species Specificity; D014018:Tissue Distribution; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology
chemical_list: D002104:Cadmium