Evaluation of the petrifilm plate method for the enumeration of aerobic microorganisms and coliforms in retailed meat samples.
This study was designed to compare the effectiveness and applicability of the Petrifilm plate method with the Association of Official Analytical Chemists' (AOAC) standard aerobic count method and violet red bile agar method for meat products. The comparison was carried out using 303 meat samples collected from various retailers: 110 pork samples, 87 chicken samples, and 107 beef samples. In the comparison of the correlation coefficient (R) between the conventional method and the Petrifilm plate method by a linear regression analysis, the correlation coefficient in total microorganisms was 0.99, 0.95, and 0.94 in pork, beef, and chicken samples, respectively. The correlation coefficient in coliform count was 0.83, 0.96, and 0.81 in pork, beef, and chicken samples, respectively. Based on the high correlation in the total microorganism count, it might be possible to replace the conventional methods with the Petrifilm plate method. For coliform counts, the Petrifilm plate method also showed a generally high correlation coefficient, except for pork samples, which are more subject to contamination. The Petrifilm plate method was simpler and less time-consuming in sample preparation and, in procedures, faster than the conventional method. These results suggested that the 3M Petrifilm plate method could replace the conventional methods in the analysis of microorganism contamination measurement in meat products.
Park
Y H
YH
Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Korea. yhp@plaza.snu.ac.kr
Seo
K S
KS
Ahn
J S
JS
Yoo
H S
HS
Kim
S P
SP
D000362:Agar; D000818:Animals; D001420:Bacteria, Aerobic / Q000302:isolation & purification*; D002417:Cattle / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods*; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D008461:Meat Products / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology; D013997:Time Factors
D000362:Agar