Molecular characterization of ionizing radiation-hypersensitive mutant M10 cells.
An ionizing radiation-sensitive mutant derivative of mouse lymphoma L5178Y cell, M10, is defective in rejoining DNA double-strand breaks (DSBs). The complementation test and the results of chromosome transfer suggested that M10 may belong to X-ray cross-complementation (XRCC) group 4. In the present study, sequence analysis of Xrcc4 cDNA in M10 cells disclosed a transversion of A (370) to T, which results in a change of arginine (124) to a termination codon. Interestingly, the mutation occurred in one allele and the transcripts of the Xrcc4 gene were expressed exclusively from the mutant allele. Transfection of M10 cells with the murine Xrcc4 cDNA completely rescued X-ray sensitivity of the mutant cells. M10 is a novel Xrcc4-deficient cell line.
Mori
M
M
Radiation Hazard Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-Ku, Chiba 263-8555, Japan. mmori@nirs.go.jp
Itsukaichi
H
H
Nakamura
A
A
Sato
K
K
D000483:Alleles; D000818:Animals; D003062:Codon / Q000235:genetics; D018389:Codon, Nonsense; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics / Q000528:radiation effects*; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D005816:Genetic Complementation Test; D007940:Leukemia L5178 / Q000235:genetics / Q000473:pathology*; D051379:Mice; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D003062:Codon; D018389:Codon, Nonsense; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human