Anxiolytic effect of BPC-157, a gastric pentadecapeptide: shock probe/burying test and light/dark test.
OBJECTIVE
To study anxiolytic effect of a gastric pentadecapeptide, BPC-157.


METHODS
In shock probe/burying test, pentadecapeptide BPC-157 (10 microg/kg, 10 ng/kg, ip), diazepam (0.075, 0.0375 mg/kg, ip), and an equivolume of saline (5 mL/kg, ip) were given at 30 min prior test. In light/dark test, the same dosage of diazepam, BPC-157, and saline were given at 45 min prior procedure.


RESULTS
Shock probe/burying test: rats treated with either diazepam or pentadecapeptide BPC-157 were much less afraid after the shock: almost not burying and the total time spent in burying was clearly less than in controls. However, while in the diazepam treated rats the number of shocks received increased over control values, in pentadecapeptide BPC-157 treated groups the number of shocks remained not modified compared with the control values. Light/dark test: after exposure to the intense light, diazepam treated mice had longer latencies of crossing to the dark compartment, a greater number of crossing and a greater number of exploratory rearing, and spent longer time in the light compartment, as compared to the control mice, while BPC-157 mice had a similar behavior to that of the control mice. In contrast with the effect in light area, in dark zone diazepam produced no change with respect to controls, while BPC-157 (10 microg/kg) mice had a greater number of crossing and a greater number of exploratory rearing.


CONCLUSIONS
Both diazepam and BPC-157 displayed a bidirectional effect, but the activity of pentadecapeptide BPC-157 was particular, and different from diazepam.
Sikiric
P
P
Department of Pharmacology, Medical Faculty University of Zagreb, Salata 11, 10000 Zagreb, Croatia. sikiric@mef.hr.
Jelovac
N
N
Jelovac-Gjeldum
A
A
Dodig
G
G
Staresinic
M
M
Anic
T
T
Zoricic
I
I
Ferovic
D
D
Aralica
G
G
Buljat
G
G
Prkacin
I
I
Lovric-Bencic
M
M
Separovic
J
J
Seiwerth
S
S
Rucman
R
R
Petek
M
M
Turkovic
B
B
Ziger
T
T
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety; D001522:Behavior, Animal / Q000187:drug effects*; D003624:Darkness; D003975:Diazepam / Q000494:pharmacology; D008027:Light; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D014151:Anti-Anxiety Agents; D010446:Peptide Fragments; D011506:Proteins; C083833:BPC 157; D003975:Diazepam