title: The Drosophila RGS protein Loco is required for dorsal/ventral axis formation of the egg and embryo, and nurse cell dumping.
abstract: The loco gene encodes members of a family of RGS proteins responsible for the negative regulation of G-protein signalling. At least two transcripts of loco are expressed in oogenesis, loco-c2 is observed in the anterior-dorsal follicle cells and is downstream of the epidermal growth factor receptor signalling pathway, initiated in the oocyte. loco-c3 is a new transcript of loco, which is expressed in the nurse cells from stage 6 onwards. Analysis of newly generated mutants and antisense technology enabled us to establish that disrupting loco in follicle cells results in ventralized eggs, while disrupting loco in nurse cells results in short eggs, due to defective dumping of the nurse cell cytoplasm into the oocyte.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, EH9 3JR, Edinburgh, UK.
authors.forename: S
authors.initials: S
authors.lastname: Pathirana
authors.forename: D
authors.initials: D
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Bownes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002874:Chromosome Mapping; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000502:physiology*; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D015723:Gene Library; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009866:Oogenesis; D010063:Ovum / Q000502:physiology*; D010641:Phenotype; D011494:Protein Kinases; D020710:RGS Proteins / Q000737:chemistry* / Q000502:physiology*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018010:Receptors, Invertebrate Peptide / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013997:Time Factors; D014018:Tissue Distribution; D014170:Transformation, Genetic; D016211:Transforming Growth Factor alpha; D015291:Transforming Growth Factors / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D019476:Insect Proteins; C119015:LOCO protein, Drosophila; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D020710:RGS Proteins; D012333:RNA, Messenger; D018010:Receptors, Invertebrate Peptide; D016211:Transforming Growth Factor alpha; C083730:grk protein, Drosophila; D012313:RNA; D015291:Transforming Growth Factors; D011494:Protein Kinases; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors