In vivo cultured skin composed of two-layer collagen sponges with preconfluent cells.
Although various kinds of cultured skin substitutes have been developed, it takes several weeks to produce them before grafting. In their previous study, the authors succeeded in producing cultured skin easily in a short period of time by layering two collagen sponges. In the current study, to shorten this period even further, they grafted the cell-preconfluent artificial skin immediately after seeding the cells. They used two collagen sponges with different pore sizes and crosslink densities. They seeded 1,000,000 cells per square centimeter of fibroblasts and 1,000,000 cells per square centimeter of keratinocytes on the respective collagen sponges and grafted them on a full-thickness, excised wound on the back of severe combined immunodeficient mice. Two weeks after grafting, epithelium and dermislike tissue were formed. They then decreased the number of keratinocytes and grafted them. Four weeks after grafting, at seeding densities of 50,000 to 1,000,000 cells per square centimeter of keratinocytes, the preconfluent artificial skin took histologically, and human type IV and type VII collagen were stained immunohistochemically. This cell-preconfluent artificial skin composed of two-layer collagen sponges seems promising for widespread clinical use.
Morimoto
N
N
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Japan.
Suzuki
S
S
Kim
B M
BM
Morota
K
K
Takahashi
Y
Y
Nishimura
Y
Y
D000818:Animals; D002478:Cells, Cultured; D024141:Collagen Type IV / Q000378:metabolism; D024143:Collagen Type VII / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology*; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology*; D051379:Mice; D016513:Mice, SCID; D017768:Skin, Artificial; D014945:Wound Healing / Q000502:physiology
D024141:Collagen Type IV; D024143:Collagen Type VII