Metabotropic purinoreceptors in rat dorsal horn neurones: predominant dendritic location.
Elevations of cytosolic free Ca2+ concentration ([Ca2+]i) induced by addition of ATP have been compared in rat dorsal horn neurones in slices and after their isolation. ATP application induced in neurones in situ a rise of [Ca2+]i by 201 +/- 12 nM. In Ca2+-free external solution the rise was 156 +/- 14 nM (n = 45 of 76), indicating the presence of active purinergic metabotropic receptors in about 59% of neurones. [Ca2+]i transients induced by 2MeSATP in Ca2+-free external solution were completely abolished by 10 microM PPADS, indicating that some of the corresponding receptors are of the P2Y1 type. In acutely isolated neurones which lost their dendrites, there were no metabotropic response. The results confirm the presence of metabotropic postsynaptic purinoreceptors located in the dendritic tree of dorsal horn neurones.
Kruglikov
I
I
Department of General Physiology of Nervous System, Bogomoletz Institute of Physiology, 4, Bogomoletz St., Kiev 01024, Ukraine.
Shutov
L
L
Potapenko
E
E
Voitenko
N
N
Kostyuk
P
P
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D020671:Posterior Horn Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D018048:Receptors, Purinergic P2 / Q000378:metabolism*
D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D002118:Calcium