Screening for differentially expressed genes in the rat inner retina and optic nerve after optic nerve crush.
Limited optic nerve crush is a model of diffuse mechanical axon injury, the most prevalent cause of secondary neurodegeneration after closed head neurotrauma. In this report, a protocol is presented which allows for the rapid screening of differential gene expression in the inner retina, as well as the optic nerve, in response to partial nerve crush. To prove the reliability of the method, prototypically, the differential expression profiles of three candidate genes (kinesin light chain, ferritin, RYB-A) were verified. The method seems to be suitable to address the question of how differential gene expression contributes to degeneration, survival and axonal repair after partial nerve crush.
Dieterich
Daniela C
DC
AG Molecular Mechanisms of Plasticity, Department of Neurochemistry/Molecular Biology, Leibniz Institute for Neurobiology, P.O. Box 1860, 39108 Magdeburg, Germany.
Böckers
Tobias M
TM
Gundelfinger
Eckart D
ED
Kreutz
Michael R
MR
D000818:Animals; D002470:Cell Survival / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D020833:Diffuse Axonal Injury / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D005293:Ferritins / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D005820:Genetic Testing / Q000379:methods*; D016547:Kinesins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009410:Nerve Degeneration / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D009416:Nerve Regeneration / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020221:Optic Nerve Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000378:metabolism* / Q000503:physiopathology; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D004268:DNA-Binding Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; C088194:Ybx3 protein, rat; D005293:Ferritins; D016547:Kinesins