title: 4-Hydroxybenzoate uptake in Klebsiella planticola strain DSZ1 is driven by DeltapH.
abstract: Klebsiella planticola strain DSZ1 has the ability to degrade different aromatic compounds such as benzoate and organochlorinated as propachlor and alachlor. DSZ1 strain cells mineralised 4-hydroxybenzoate (4HBA) through a meta-cleavage pathway, yielding protocatechuate as dihydroxylated intermediate, with a specific rate of CO2 formation 0.12 x 10-6 (cpm/OD) h-1, and a rate of 4-HBA utilisation of 0.75 mmol h-1. Aerobically the 4HBA transport system is driven by gradient of protons (DeltapH), but is not ATP-driven. Under anaerobic conditions, the system can use the nitrate reduction as a final electron acceptor in respiration. A kinetic analysis of the 4HBA transport system revealed a Kt value of 16 microM with a Vmax value of 25 nmol/min.mg at pH 7.
authors.affiliation: Dpt. Fisica Aplicada, Universidad Complutense, Facultad de Veterinaria, Avda. Puerta de Hierro s/n. 28040 Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Allende
authors.forename: A
authors.initials: A
authors.lastname: Gibello
authors.forename: A
authors.initials: A
authors.lastname: Fortún
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Martín
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D001565:Benzoates / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D010226:Parabens / Q000378:metabolism*; D019810:Sodium Azide / Q000378:metabolism
chemical_list: D001565:Benzoates; D010226:Parabens; D000255:Adenosine Triphosphate; D019810:Sodium Azide