title: Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells.
abstract: OBJECTIVE
  To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304).
  
  
  METHODS
  VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization.
  
  
  RESULTS
  Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time- and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells.
  
  
  CONCLUSIONS
  LPC could induce a strong expression of VEGF in ECV304 cells.
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Second Military Medical University, Shanghai 200433, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: Y C
authors.initials: YC
authors.lastname: Rui
authors.forename: Y
authors.initials: Y
authors.lastname: Qiu
authors.forename: T J
authors.initials: TJ
authors.lastname: Li
mesh_terms: D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D008244:Lysophosphatidylcholines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A