title: Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human dendritic cells enhances recombinant adenovirus-mediated gene transfer.
abstract: Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central modulators of cellular immune responses. Genetic modification of DCs has considerable therapeutic potential in the treatment of a wide spectrum of diseases, including cancer and persistent viral infection. In this report, we show that pre-treatment of DCs with a recombinant adenovirus encoding the major adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly increased the uptake of recombinant adenoviruses (Ads) by primary immature monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein expression. Transduction of DCs by recombinant adenoviruses did not significantly alter cellular viability. Therefore, we propose that pre-treatment of DCs with Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by recombinant Ads.
authors.affiliation: School of Biochemistry and Molecular Biology, Mount Preston Street, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Luke H
authors.initials: LH
authors.lastname: Stockwin
authors.forename: Torkjel
authors.initials: T
authors.lastname: Matzow
authors.forename: Nikolaos T
authors.initials: NT
authors.lastname: Georgopoulos
authors.forename: Lindsay J
authors.initials: LJ
authors.lastname: Stanbridge
authors.forename: Samantha V
authors.initials: SV
authors.lastname: Jones
authors.forename: Iain G
authors.initials: IG
authors.lastname: Martin
authors.forename: Maria E
authors.initials: ME
authors.lastname: Blair-Zajdel
authors.forename: G Eric
authors.initials: GE
authors.lastname: Blair
mesh_terms: D017951:Antigen Presentation / Q000235:genetics* / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D016865:Reassortant Viruses / Q000235:genetics* / Q000276:immunology; D011991:Receptors, Virus / Q000235:genetics* / Q000276:immunology; D014162:Transfection / Q000379:methods
chemical_list: D011991:Receptors, Virus; C085301:adenovirus receptor; C105941:coxsackievirus B receptor