title: Stem cell transplantation eliminates alloantibody in a highly sensitized patient.
abstract: Highly sensitized patients are forced to stay on transplant waiting lists for many years and ultimately may never find a donor. Peripheral blood stem cell (PBSC) transplantation may provide a strategy to decrease host alloreactivity through the production of a chimeric state. We investigated alloreactivity and chimerism in a highly sensitized 40-year-old patient with sickle cell disease who underwent a nonradiation based conditioning regimen consisting of fludarabine, ATG, and high dose melphalan, for allogeneic stem cell transplant. Host monocytes and lymphocytes became donor in origin by day 14. PRA, initially 100% pretransplant, fell to 0 by day 263. Anti-red blood cells antibody became undetectable by day 152. The use of a new nonradiation-based conditioning regimen enabled successful engraftment of allogeneic donor PBSCs and the elimination of alloantibody. As new less toxic conditioning regimens are developed, PBSC transplantation might provide a new solution to allosensitization.
authors.affiliation: University of Illinois at Chicago Department of Surgery, Division of Transplantation (M/C 958), 840 South Wood Street, Chicago, IL 60612, USA.
authors.forename: A
authors.initials: A
authors.lastname: Bartholomew
authors.forename: D
authors.initials: D
authors.lastname: Sher
authors.forename: S
authors.initials: S
authors.lastname: Sosler
authors.forename: W
authors.initials: W
authors.lastname: Stock
authors.forename: V
authors.initials: V
authors.lastname: Lazda
authors.forename: M
authors.initials: M
authors.lastname: Koshy
authors.forename: S
authors.initials: S
authors.lastname: Devine
authors.forename: K
authors.initials: K
authors.lastname: vanBesien
mesh_terms: D000328:Adult; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008168:Lung / Q000503:physiopathology; D019172:Transplantation Conditioning
chemical_list: D007518:Isoantibodies