title: Endogenous production of protoporphyrin IX induced by 5-aminolevulinic acid in leukemia cells.
abstract: OBJECTIVE
  To explore the photosensitization of 5-aminolevulinic acid (ALA) in myeloid leukemia cell line.
  
  
  METHODS
  Using the technique of fluorescence spectra, the A LA induced protoporphyrin IX (Pp IX) was measured in myeloid leukemia JCS cells. Cofocal laser scanning microscopy (CLSM) combined with fluorescence organelle probe was used to detect the localization of Pp IX in JCS cells at the subcellular levels. MTT assay was used to measure the cell survival after light irradiation.
  
  
  RESULTS
  ALA successfully produced endogenous PpI X in leukemia JCS cells. PpIX was observed to be distributed in the cytoplasm and mitochondria was exhibited as the one of binding sites of PpIX. As a photosensitizer, PpIX initiated photodynamic reaction after light irradiation and effectively photodamaged leukemia cells.
  
  
  CONCLUSIONS
  ALA-based photosensitization could be used for inactivation of leukemia cells.
authors.affiliation: Department of Physics, Fudan University, Shanghai 200433, China. jychen@fudan.edu.cn.
authors.forename: J Y
authors.initials: JY
authors.lastname: Chen
authors.forename: N Q
authors.initials: NQ
authors.lastname: Mak
authors.forename: N H
authors.initials: NH
authors.lastname: Cheung
authors.forename: R N
authors.initials: RN
authors.lastname: Leung
authors.forename: Q
authors.initials: Q
authors.lastname: Peng
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D000818:Animals; D007942:Leukemia, Experimental / Q000378:metabolism* / Q000473:pathology; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX