Conformational and functional significance of residue proline 17 in chicken muscle adenylate kinase.
The effect of mutation proline 17 on the multiple conformations and catalytic function in chicken muscle adenylate kinase (AK) has been studied. The substitution of proline 17 with glycine or valine altered the distribution of multiple conformations. Compared with the wild-type enzyme, the P17G and P17V mutants contained decreased fraction of minor conformer from 18% to 9% and 11%, respectively. Due to the mutation, the enzyme showed lower secondary structural content, poorer affinity to substrates or substrate analogues, and reduced catalytic efficiency. The results revealed the significance of proline 17 in the conformation and function of AK.
Sheng
X
X
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, PR China. xrsheng@sun5.ibp.ac.cn
Pan
X
X
Wang
C
C
Zhang
Y
Y
Jing
G
G
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002645:Chickens; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005998:Glycine / Q000737:chemistry; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D016297:Mutagenesis, Site-Directed; D011392:Proline / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D014633:Valine / Q000737:chemistry
D000817:Anilino Naphthalenesulfonates; D015226:Dinucleoside Phosphates; D004791:Enzyme Inhibitors; D000249:Adenosine Monophosphate; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000244:Adenosine Diphosphate; C027132:1-anilino-8-naphthalenesulfonate; D000255:Adenosine Triphosphate; D011392:Proline; D000263:Adenylate Kinase; D014633:Valine; D005998:Glycine