title: RNA ligands generated against complex nuclear targets indicate a role for U1 snRNP in co-ordinating transcription and RNA splicing.
abstract: RNA ligands were generated against various gene products present in HeLa nuclear extract. Functional profiling was performed to identify RNA ligands that modulate RNA polymerase II (pol II)-mediated transcription. Unexpectedly, four of the eight inhibitor ligands identified by this screen contained an 11-nucleotide sequence identical to the 5'-splice site of eukaryotic pre-mRNAs. Such ligands were shown to impede pre-initiation complex assembly on a cytomegalovirus promoter. In addition, U1 small nuclear ribonucleoprotein particles (snRNP) and pol II had been co-immunoprecipitated in the absence of transcription. These results suggest a role for U1 snRNP in co-ordinating transcription and RNA splicing.
authors.affiliation: Department of Genetics, Center for Genetic and Cellular Therapies, Duke University Medical Center, Durham, NC 27710, USA. htian@alumnidirector.com
authors.forename: H
authors.initials: H
authors.lastname: Tian
mesh_terms: D001483:Base Sequence; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D019384:Nucleic Acid Synthesis Inhibitors; D012319:RNA Polymerase II / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D008024:Ligands; D019384:Nucleic Acid Synthesis Inhibitors; D012322:RNA Precursors; D017412:Ribonucleoprotein, U1 Small Nuclear; D012319:RNA Polymerase II