title: 2-Amino-7-deazaadenine forms stable base pairs with cytosine and thymine.
abstract: 2-Amino-7-deazaadenine ((AD)A) was incorporated into oligodeoxynucleotides (ODN) and their base-pairing properties with natural nucleobases were investigated. In melting temperature (T(m)) experiments, the duplex containing an (AD)A/C base pair showed a high stability comparable to that containing (AD)A/T base pair. Destabilization of the duplex usually observed for existing degenerate bases was not observed. However, the incorporation efficiency of dCTP was only 1.8% for TTP in single-nucleotide insertion reactions using polymerase.
authors.affiliation: Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and CREST, Japan.
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry*; D001482:Base Composition; D020029:Base Pairing; D003596:Cytosine / Q000737:chemistry*; D004355:Drug Stability; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D013696:Temperature; D013941:Thymine / Q000737:chemistry*
chemical_list: C465968:2-amino-7-deazaadenine; D009838:Oligodeoxyribonucleotides; C043837:7-deazaadenine; D003596:Cytosine; D000225:Adenine; D013941:Thymine