title: Delayed expression of the NFH subunit in differentiating P19 cells.
abstract: Pluripotent embryonal carcinoma (P19) cells differentiate into a neural phenotype in response to retinoic acid (RA). Expression of the low and medium molecular weight neurofilament subunits, but not the high molecular weight subunit (NFH), has been reported following RA treatment. In this study NFH expression was detected by Western blotting and immunofluorescence microscopy, but lagged behind the expression of the other subunits in a manner similar to that reported during in vivo neuronal development.
authors.affiliation: Biology Department, University of North Dakota, P.O. Box 9019, Grand Forks, ND 58202-9019, USA. sally_pyle@und.nodak.edu
authors.forename: S J
authors.initials: SJ
authors.lastname: Pyle
authors.forename: K G
authors.initials: KG
authors.lastname: Roberts
authors.forename: K R
authors.initials: KR
authors.lastname: Reuhl
mesh_terms: D000818:Animals; D000906:Antibodies; D018236:Carcinoma, Embryonal; D002454:Cell Differentiation; D002465:Cell Movement; D019084:Fluorescent Antibody Technique, Indirect; D016501:Neurites / Q000378:metabolism; D016900:Neurofilament Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure*; D013724:Teratoma; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D016900:Neurofilament Proteins; C051774:neurofilament protein H