title: A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.
abstract: The structures of a series of complexes designed to mimic intermediates along the reaction coordinate for beta-galactosidase are presented. These complexes clarify and enhance previous proposals regarding the catalytic mechanism. The nucleophile, Glu537, is seen to covalently bind to the galactosyl moiety. Of the two potential acids, Mg(2+) and Glu461, the latter is in better position to directly assist in leaving group departure, suggesting that the metal ion acts in a secondary role. A sodium ion plays a part in substrate binding by directly ligating the galactosyl 6-hydroxyl. The proposed reaction coordinate involves the movement of the galactosyl moiety deep into the active site pocket. For those ligands that do bind deeply there is an associated conformational change in which residues within loop 794-804 move up to 10 A closer to the site of binding. In some cases this can be inhibited by the binding of additional ligands. The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation.
authors.affiliation: Institute of Molecular Biology, Howard Hughes Medical Institute and Department of Physics, University of Oregon, Eugene, Oregon 97403-1229, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Juers
authors.forename: T D
authors.initials: TD
authors.lastname: Heightman
authors.forename: A
authors.initials: A
authors.lastname: Vasella
authors.forename: J D
authors.initials: JD
authors.lastname: McCarter
authors.forename: L
authors.initials: L
authors.lastname: Mackenzie
authors.forename: S G
authors.initials: SG
authors.lastname: Withers
authors.forename: B W
authors.initials: BW
authors.lastname: Matthews
mesh_terms: D001665:Binding Sites; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006860:Hydrogen Bonding; D007763:Lac Operon; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D001616:beta-Galactosidase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008024:Ligands; D001616:beta-Galactosidase