Chimpanzee adenovirus CV-68 adapted as a gene delivery vector interacts with the coxsackievirus and adenovirus receptor.
A replication-defective form of chimpanzee adenovirus type 68 (C68) has been developed to circumvent problems posed by widespread preexisting immunity to common human adenovirus vectors. To investigate the determinants of C68 tropism, its interaction with the coxsackievirus and adenovirus receptor (CAR) was studied. Although CHO cells were resistant to transduction by C68 as well as by adenovirus type 5 (Ad5), CHO cells expressing either human or murine CAR were transduced readily. C68 transduction, like Ad5 transduction, was blocked when cells were exposed to anti-CAR antibody or when virus was exposed to a soluble form of the CAR extracellular domain. These results indicate that gene delivery by C68 occurs by a CAR-dependent mechanism.
Cohen
Christopher J
CJ
The Children's Hospital of Philadelphia, Division of Immunologic and Infectious Diseases, Abramson 1202, 3516 Civic Center Boulevard, Philadelphia, PA 19104, USA1.
Xiang
Zhi Quan
ZQ
The Wistar Institute, Philadelphia, PA 19104, USA2.
Gao
Guang-Ping
GP
The Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA3.
Ertl
Hildegund C J
HCJ
The Wistar Institute, Philadelphia, PA 19104, USA2.
Wilson
James M
JM
The Institute for Human Gene Therapy, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA3.|The Wistar Institute, Philadelphia, PA 19104, USA2.
Bergelson
Jeffrey M
JM
The Children's Hospital of Philadelphia, Division of Immunologic and Infectious Diseases, Abramson 1202, 3516 Civic Center Boulevard, Philadelphia, PA 19104, USA1.
D000222:Adaptation, Physiological; D000260:Adenoviruses, Human / Q000378:metabolism*; D000261:Adenoviruses, Simian / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D006224:Cricetinae; D004770:Enterovirus / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014161:Transduction, Genetic
C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D008164:Luminescent Proteins; D011991:Receptors, Virus; D049452:Green Fluorescent Proteins