Association of mouse sorting nexin 1 with early endosomes.
Sorting nexin 1 (SNX1) is a protein that binds to the cytoplasmic domain of plasma membrane receptors. We found that mouse sorting nexin 1 (SNX1) (521 amino acid residues) could partially rescue a yeast vam3 mutant defective in docking/fusion of vacuolar membranes. In mammalian cells, SNX1 is peripherally associated with membrane structures and localized immunochemically with EEA1, a marker protein of early endosomes. These results suggest that SNX1 regulates endocytic trafficking of plasma membrane proteins in early endosomes. Gel filtration of cell lysates and the purified recombinant protein, together with two-hybrid analysis, indicated that SNX1 self-assembles into a complex of approximately 300 kDa.
Nakamura
N
N
Division of Biological Sciences, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
Sun-Wada
G H
GH
Yamamoto
A
A
Wada
Y
Y
Futai
M
M
D016475:3T3 Cells; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D016466:CHO Cells; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D011992:Endosomes / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts; D007425:Intracellular Membranes / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism*; D008647:Mesocricetus; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D021381:Protein Transport / Q000502:physiology; D014018:Tissue Distribution / Q000502:physiology; D033921:Vesicular Transport Proteins; D015003:Yeasts / Q000235:genetics
D015415:Biomarkers; D002352:Carrier Proteins; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; C093846:early endosome antigen 1