title: Ultra-thin-polysiloxane-film-composite membranes for the optimisation of amperometric oxidase enzyme electrodes.
abstract: An outer ultra-thin-polydimethyldichlorosiloxane film composite membrane has been used as the outer covering barrier in an amperometric glucose oxidase enzyme electrode biosensor. The composite membrane was formed via the condensation polymerisation of dimethyldichlorosilane at the surface of a host porous alumina membrane. Homogeneous polydimethyldichlorosiloxane films of <100 nm thickness acted as effective substrate diffusional barriers and were supported by the underlying porous alumina surface. Glucose and oxygen permeability coefficients were determined using diffusion chamber apparatus. Polysiloxane composite membranes were found to offer some screening functionality towards anionic biological interferents such as ascorbate. On exposure to blood an approximate 25% signal drift was observed during the first 2 h exposure to blood; after this time responses remained almost stable. Whole blood glucose determinations showed a close correlation (r(2)=0.98) to analyses performed via standard hospital analyses.
authors.affiliation: Manchester Materials Science Centre, University of Manchester and UMIST, Grosvenor Street, Manchester M1 7HS, UK.
authors.forename: S
authors.initials: S
authors.lastname: Myler
authors.forename: S D
authors.initials: SD
authors.lastname: Collyer
authors.forename: K A
authors.initials: KA
authors.lastname: Bridge
authors.forename: S P J
authors.initials: SP
authors.lastname: Higson
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D012833:Siloxanes
chemical_list: D001786:Blood Glucose; D012833:Siloxanes; D005949:Glucose Oxidase