Examination of cross-antigenicity of acetylcholinesterase and butyrylcholinesterase using anti-acetylcholinesterase antibodies.
Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) share a high degree of homology and overlap in several biochemical properties. This study aimed to compare and contrast the antigenic reactivity of AChE and BuChE with several polyclonal antibodies. We have performed a detailed analysis of AChE and BuChE enzymatic activities with different substrates and different inhibitors. Immunoassays conducted with polyclonal amino-terminus-specific anti-AChE antibodies were selective for mouse and electric eel AChE (EEAChE). Polyclonal carboxy-terminus-specific anti-AChE antibodies reacted with EEAChE and human BuChE, indicating an unexpected cross-reactivity. Polyclonal antisera raised against the whole AChE protein cross-reacted with horse BuChE, but not human BuChE. These data demonstrate that AChE and BuChE are immunologically similar.
George
Kathleen M
KM
Department of Pharmaceutical Sciences, Center for Environmental Health Science, University of Montana, Missoula, MT 59812, USA.
Montgomery
Melody A
MA
Sandoval
Lisa E
LE
Thompson
Charles M
CM
D000110:Acetylcholinesterase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D015153:Blotting, Western; D002091:Butyrylcholinesterase / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins; D013045:Species Specificity; D013379:Substrate Specificity
D002800:Cholinesterase Inhibitors; D011994:Recombinant Proteins; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase