Effect of MITF on transcription of transmembrane tryptase gene in cultured mast cells of mice.
Mouse mast cell protease (mMCP)-6, mMCP-7 and transmembrane tryptase (TMT) are all tryptases. The normal mi transcription factor (+-MITF) transactivated mMCP-6 gene by binding three consensus motifs in the promoter region, but no MITF-binding motifs were found in the mMCP-7 promoter. Instead, c-Jun transactivated mMCP-7 gene, and +-MITF cooperated with it. The mi-MITF encoded by mutant mi allele inhibited the transactivation by c-Jun and reduced the mMCP-7 promoter activity. Here, the effect of MITF on the TMT gene expression was examined. The +-MITF enhanced the TMT promoter activity by binding two consensus motifs. The mi-MITF showed the inhibitory effect on TMT gene expression. The effect of +-MITF on TMT gene was similar to the effect on mMCP-6 gene, and that of mi-MITF was similar to the effect on mMCP-7 gene. The effects of MITF on TMT gene appeared distinct from its effects on either mMCP-6 or mMCP-7 gene.
Morii
E
E
Department of Pathology, Osaka University Medical School, Suita, Japan. morii@patho.med.osaka-u.ac.jp
Ogihara
H
H
Oboki
K
K
Kataoka
T R
TR
Jippo
T
T
Kitamura
Y
Y
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D051739:Microphthalmia-Associated Transcription Factor; D011401:Promoter Regions, Genetic; D012697:Serine Endopeptidases / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection; D053802:Tryptases
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; C509190:Tpsb2 protein, mouse; D014157:Transcription Factors; D012697:Serine Endopeptidases; C509222:Tpsab1 protein, mouse; D053802:Tryptases