title: Regulation of articular chondrocyte phenotype by bone morphogenetic protein 7, interleukin 1, and cellular context is dependent on the cytoskeleton.
abstract: Bone morphogenetic proteins (BMPs) induce cartilage differentiation and morphogenesis. There are profound changes in the cytoskeletal architecture during the morphogenesis of cartilage. To investigate the possibility that morphogenetic signals such as BMPs may regulate chondrocyte phenotype by modulation of cytoskeletal protein expression, we determined whether the expression and distribution of cytoskeletal proteins in chondrocytes are regulated by bone morphogenetic protein 7 (BMP 7), interleukin 1 (IL-1), and cellular context. Addition of BMP 7, a morphogen that induces chondrogenesis, to primary cultures of bovine and murine chondrocytes induced increased expression of four cytoskeletal proteins: tensin, talin, paxillin, and focal adhesion kinase (FAK). The expression of cytoskeletal proteins is dependent on cellular context; compared to monolayer, chondrocytes in suspension exhibited increased expression of cytoskeletal components. Conversely, addition of IL-1, a catabolic cytokine, induced loss of chondrocyte phenotype and decreased the expression of these cytoskeletal components. Treatment of chondrocytes with cytochalasin D (an agent that disrupts the actin cytoskeleton) inhibited BMP 7-induced upregulation of tensin, talin, paxillin, and FAK, and blocked the effect of BMP 7 on chondrocyte phenotype. Taken together these data demonstrate that cytoskeletal components play a critical role in the response to morphogens and cytokines in the regulation of chondrocyte phenotype. (c)2001 Elsevier Science.
authors.affiliation: Center for Tissue Regeneration and Repair, University of California Davis, Sacramento, California 95817, USA. ruth.vinall@ucdmc.ucdavis.edu
authors.forename: Ruth L
authors.initials: RL
authors.lastname: Vinall
authors.forename: Su Hao
authors.initials: SH
authors.lastname: Lo
authors.forename: A Hari
authors.initials: AH
authors.lastname: Reddi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology / Q000502:physiology*; D002358:Cartilage, Articular / Q000254:growth & development*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D003571:Cytochalasin B / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D015536:Down-Regulation / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009024:Morphogenesis; D016212:Transforming Growth Factor beta; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D003598:Cytoskeletal Proteins; D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D003571:Cytochalasin B