The effects of hypo- and hyperthermic pretreatment on sodium taurocholate-induced acute pancreatitis in rats.
BACKGROUND
Heat shock proteins (HSPs) have indispensable functions in the synthesis, degradation, folding, transport, and translocation of intracellular proteins. HSPs are proteins that help cells to survive stress conditions by repairing damaged proteins.


OBJECTIVE
To investigate the potential effects of HSP preinduction by cold-water (CWI) or hot-water immersion (HWI) on sodium taurocholate (TC)-induced acute pancreatitis in rats.


METHODS
TC was injected into the common biliopancreatic duct of the animals at the peak level of HSP synthesis, as determined by Western blot analysis. The rats were killed by exsanguination through the abdominal aorta 6 hours after the TC injection. The serum amylase activity, the IL-1, IL-6 and TNF-alpha levels, the pancreatic weight/body weight ratio, and the pancreatic contents of DNA, protein, amylase, lipase, and trypsinogen were measured, and a biopsy for histology was taken.


RESULTS
HWI significantly elevated HSP72 expression, whereas CWI significantly increased HSP60 expression. It was demonstrated that CWI pretreatment ameliorated the pancreatic edema and the serum amylase level increase, whereas the morphologic damage was more severe in this form of acute pancreatitis. HWI pretreatment did not have any effects on the measured parameters in TC-induced pancreatitis.


CONCLUSIONS
The findings suggest a possible role of HSP60, but not HSP72, in the slight protection in the early phase of this necrohemorrhagic pancreatitis model.
Rakonczay
Zoltán
Z
First Department of Medicine, University of Szeged, Szeged, Hungary. raz@int1.szote.u-szeged.hu
Takács
Tamás
T
Iványi
Béla
B
Mándi
Yvette
Y
Pápai
Gábor
G
Boros
Imre
I
Varga
Ilona Sz
IS
Jost
Krisztina
K
Lonovics
János
J
D000681:Amylases / Q000097:blood; D000818:Animals; D001835:Body Weight; D018834:Chaperonin 60 / Q000032:analysis; D016207:Cytokines / Q000097:blood; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D005334:Fever / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis; D007035:Hypothermia / Q000378:metabolism; D008049:Lipase / Q000032:analysis; D008297:Male; D009929:Organ Size; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid; D014362:Trypsinogen / Q000032:analysis
D018834:Chaperonin 60; D016207:Cytokines; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D013656:Taurocholic Acid; D014362:Trypsinogen; D004247:DNA; D008049:Lipase; D000681:Amylases