title: Haemonchus contortus utilises catalase in defence against exogenous hydrogen peroxide in vitro.
abstract: The toxicity of activated oxygen species towards adult Haemonchus contortus nematodes was examined in in vitro assays using ingestion of [3H]inulin to assess nematode viability. Both glucose/glucose oxidase (generation of hydrogen peroxide) and xanthine/xanthine oxidase (generation of superoxide anion) systems showed concentration-dependent toxicity to the nematodes. Both adult and larval Haemonchus contortus enzyme preparations showed significant catalase activities. Adult nematodes exposed to aminotriazole for 24 h showed catalase activities reduced to less than 20% of controls. Aminotriazole-treated nematodes exposed to a glucose/glucose oxidase system were significantly more susceptible to the toxic effects of the oxidant-generating system than controls (no aminotriazole pre-treatment). The concentration of glucose oxidase required to inhibit feeding by 50% was decreased 33-fold in aminotriazole-treated nematodes compared with controls. The effect of aminotriazole pre-treatment implicates hydrogen peroxide as a significant toxic agent in the glucose/glucose oxidase system. It is apparent that inhibition of Haemonchus contortus catalase increases the susceptibility of the parasite to the toxic effects of hydrogen peroxide, demonstrating a protective role for this enzyme. This suggests that catalase has the potential to play a significant role in the defence of this parasite against hydrogen peroxide produced as part of the respiratory burst of activated phagocytes within the host during its response to nematode infection.
authors.affiliation: CSIRO Livestock Industries, Locked Bag 1 Delivery Centre, Blacktown, NSW, 2148, Australia. andrew.kotze@li.csiro.au
authors.forename: A C
authors.initials: AC
authors.lastname: Kotze
authors.forename: S J
authors.initials: SJ
authors.lastname: McClure
mesh_terms: D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005949:Glucose Oxidase / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism; D006189:Haemonchus / Q000187:drug effects / Q000201:enzymology*; D006861:Hydrogen Peroxide / Q000032:analysis / Q000494:pharmacology*; D007444:Inulin / Q000493:pharmacokinetics; D013482:Superoxide Dismutase / Q000378:metabolism; D014969:Xanthine Oxidase / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D007444:Inulin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D005982:Glutathione Transferase; D000640:Amitrole