title: Lack of enhancing effect of two Kampo medicines, Sho-saiko-to (TJ-9) and Sairei-to (TJ-114), on rat urinary bladder carcinogenesis initiated with N-butyl-N-(4-hydroxybutyl)nitrosamine.
abstract: The modifying potential of two Kampo medicines (Japanese traditional herbal medicines), Sho-saiko-to (TJ-9) and Sairei-to (TJ-114), on urinary bladder carcinogenesis in male F344 rats initiated with N-butyl-N-(4-hydroxybutyl)- nitrosamine (BBN) was evaluated. Groups of 20 animals were given 0.05% BBN in their drinking water for 4 weeks and then 0.7 or 2.8% TJ-9, 0.9 or 3.6% TJ-114, or 3.0% sodium bicarbonate (NaHCO(3)) as a positive control substance in their diet for 32 weeks. All rats were killed after 36 weeks and examined histopathologically. No adverse effects of the test compounds were found in terms of survival, clinical sign, and body weight. Administration of 0.7 and 2.8% TJ-9 and 0.9 and 3.6% TJ-114 in the diet did not affect the incidences or extent of PN hyperplasia in the BBN-treated rats. Incidences and multiplicities of papillomas were also not affected in rats fed 0.7 or 2.8% TJ-9 and 0.9% TJ-114, while they were significantly decreased in animals given 3.6% TJ-114 in the diet. The results thus demonstrated that neither of the test chemicals exerted any promotional activity on urinary bladder carcinogenesis, in clear contrast to NaHCO(3). In addition, bladder carcinogenesis was reduced by 3.6% TJ-114 in the diet, under the present experimental conditions.
authors.affiliation: Daiyu-kai Institute of Medical Science, Ichinomiya, Japan. hagiwara@daiyu-kai.com
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hagiwara
authors.forename: Masashi
authors.initials: M
authors.lastname: Sano
authors.forename: Hikaru
authors.initials: H
authors.lastname: Tanaka
authors.forename: Mayumi
authors.initials: M
authors.lastname: Kawabe
authors.forename: Seiko
authors.initials: S
authors.lastname: Tamano
authors.forename: Tadaomi
authors.initials: T
authors.lastname: Kadota
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yanagisawa
authors.forename: Shunichi
authors.initials: S
authors.lastname: Maemura
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Ito
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shirai
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002085:Butylhydroxybutylnitrosamine / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006965:Hyperplasia; D008297:Male; D010212:Papilloma / Q000139:chemically induced* / Q000473:pathology / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344; D013256:Steroids; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000652:urine
chemical_list: D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D013256:Steroids; C080903:sairei-to; D002085:Butylhydroxybutylnitrosamine; C018142:saiko-keishi-to