title: Facilitation of calmodulin-mediated odor adaptation by cAMP-gated channel subunits.
abstract: Calcium (Ca2+) influx through Ca2+-permeable ion channels plays a pivotal role in a variety of neuronal signaling processes, and negative-feedback control of this influx by Ca2+ itself is often equally important for modulation of such signaling. Negative modulation by Ca2+ through calmodulin (CaM) on cyclic nucleotide-gated (CNG) channels underlies the adaptation of olfactory receptor neurons to odorants. We show that this feedback requires two additional subunits of the native olfactory channel, CNGA4 and CNGB1b, even though the machinery for CaM binding and modulation is present in the principal subunit CNGA2. This provides a rationale for the presence of three distinct subunits in the native olfactory channel and underscores the subtle link between the molecular make-up of an ion channel and the physiological function it subserves.
authors.affiliation: Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, 52425 Jülich, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Bradley
authors.forename: D
authors.initials: D
authors.lastname: Reuter
authors.forename: S
authors.initials: S
authors.lastname: Frings
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D020013:Calcium Signaling; D002147:Calmodulin / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels; D025461:Feedback, Physiological; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010782:Photolysis; D021122:Protein Subunits; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D002147:Calmodulin; D054815:Cyclic Nucleotide-Gated Cation Channels; D007473:Ion Channels; D021122:Protein Subunits; D011994:Recombinant Proteins; D000242:Cyclic AMP; D002118:Calcium