title: Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells.
abstract: Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 microM, but not dose-dependent from 0.1 to 20 microM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.
authors.affiliation: Department of Medical Radioscience, Faculty of Health Sciences, Okayama University Medical School, Okayama 700-8558, Japan. kshibuya@md.okayama-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Shibuya
authors.forename: Shoji
authors.initials: S
authors.lastname: Kawasaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kuroda
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takemoto
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Asaumi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hiraki
mesh_terms: D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006358:Hot Temperature / Q000009:adverse effects*; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008647:Mesocricetus; D009183:Mycotoxins / Q000494:pharmacology; D000081082:Phosphoinositide-3 Kinase Inhibitors; D013312:Stress, Physiological; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D004791:Enzyme Inhibitors; D009183:Mycotoxins; D000081082:Phosphoinositide-3 Kinase Inhibitors; D000077191:Wortmannin