title: In vitro microbicidal activities of cecropin peptides D2A21 and D4E1 and gel formulations containing 0.1 to 2% D2A21 against Chlamydia trachomatis.
abstract: Topically applied microbicides that eradicate pathogens at the time of initial exposure represent a powerful strategy for the prevention of sexually transmitted infections. To aid in the further development of an effective topical microbicide, we assessed the minimum cidal concentration (MCC) of two cecropin peptides, D2A21 and D4E1, and gel formulations containing 0.1 to 2% D2A21 against Chlamydia trachomatis in vitro. The MCC of peptide D2A21 was 5 microM (18.32 microg/ml), and that of peptide D4E1 was 7.5 microM (21.69 microg/ml). The MCC of gel formulations containing 2% D2A21 was 0.2 mM (0.7 mg/ml), and that of gel formulations containing 0.5% D2A21 was 0.2 mM (0.7 mg/ml). There was no significant variation in the results when two different C. trachomatis strains were tested, and the addition of 10% human blood did not significantly alter the MCCs. pH values above and below 7 reduced the activity of the D2A21 peptide alone, but the MCC of the 2% D2A21 gel formulation was only slightly altered at the various pHs tested. Ultrastructural studies indicated that C. trachomatis membranes were disrupted after D2A21 exposure, resulting in leakage of the cytoplasmic contents. These in vitro results suggest that these cecropin peptides may be an effective topical microbicide against C. trachomatis and support the need for further evaluation.
authors.affiliation: Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Ballweber
authors.forename: J E
authors.initials: JE
authors.lastname: Jaynes
authors.forename: W E
authors.initials: WE
authors.lastname: Stamm
authors.forename: M F
authors.initials: MF
authors.lastname: Lampe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D054807:Cecropins; D002626:Chemistry, Pharmaceutical; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000648:ultrastructure; D004396:Coloring Agents / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D010078:Oxazines; D010455:Peptides; D014966:Xanthenes
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054807:Cecropins; D004396:Coloring Agents; C430264:D2A21; C443753:D4E1 peptide; D010078:Oxazines; D010455:Peptides; D014966:Xanthenes; C005843:resazurin