title: Blepharismins, produced by the protozoan, Blepharisma japonicum, form ion-permeable channels in planar lipid bilayer membranes.
abstract: Blepharismins are polycyclic quinones found in the pigment granules of the ciliated protozoan, Blepharisma. Exposure to purified blepharismins results in lethal damage to several other ciliates. We here report that, at cytotoxic concentrations, blepharismins formed cation-selective channels in planar phospholipid bilayer membranes. The channels formed in a diphytanoylphosphatidylcholine bilayer had a K(+)/Cl(-) permeability ratio of 6.6:1. Single channel recordings revealed the conductance to be quite heterogeneous, ranging from 0.2 to 2.8 nS in solutions containing 0.1 M KCl, possibly reflecting different states of aggregation of blepharismin. Our observations suggest that channel formation is a cytotoxic mechanism of blepharismin's action against predatory protozoa.
authors.affiliation: Department of Basic Health Science and Fundamental Nursing, Gifu University School of Medicine, Japan. ymuto@cc.gifu-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: T
authors.initials: T
authors.lastname: Matsuoka
authors.forename: A
authors.initials: A
authors.lastname: Kida
authors.forename: Y
authors.initials: Y
authors.lastname: Okano
authors.forename: Y
authors.initials: Y
authors.lastname: Kirino
mesh_terms: D000818:Animals; D002712:Chlorides / Q000378:metabolism; D016798:Ciliophora / Q000378:metabolism*; D004553:Electric Conductivity; D004594:Electrophysiology; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers; D010539:Permeability; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D010713:Phosphatidylcholines; D011188:Potassium / Q000378:metabolism
chemical_list: D002712:Chlorides; D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; C092293:blepharismin; C021016:1,2-diphytanoylphosphatidylcholine; D010569:Perylene; D011188:Potassium