Sustained release of human growth hormone (hGH) from collagen film and evaluation of effect on wound healing in db/db mice.
Collagen films containing human growth hormone (hGH) were prepared and the release of hGH from these films and their effect on healing of full-thickness wounds in db/db mice were evaluated. The release profiles of hGH from the collagen films varied with composition and preparation conditions. The film prepared by air-drying of the mixture of hGH and collagen solution released hGH continuously over 3 days both in vitro and in vivo. By application of collagen film containing 3 mg of hGH twice at an interval of 6 days to wounds, area of wounds on day 21 was significantly reduced compared with that of non-treated wounds. Application of hGH alone at the same dose had no significant effect on wound healing. The maximum serum hGH concentration after single administration of the hGH collagen film was lower than that with hGH alone, and hGH persisted in serum over 3 days. These results suggest that hGH collagen film may be a useful topical formulation for the treatment of wounds.
Maeda
M
M
Formulation Research Laboratories, Research Center, Sumitomo Pharmaceuticals Co. Ltd., 3-45, Kurakakiuchi 1-chome, Ibaraki, 567-0878, Osaka, Japan. maedam@sumitomopharm.co.jp
Kadota
K
K
Kajihara
M
M
Sano
A
A
Fujioka
K
K
D000818:Animals; D002626:Chemistry, Pharmaceutical; D003094:Collagen / Q000008:administration & dosage* / Q000493:pharmacokinetics; D003692:Delayed-Action Preparations / Q000493:pharmacokinetics; D004353:Drug Evaluation, Preclinical / Q000379:methods; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D003692:Delayed-Action Preparations; D019382:Human Growth Hormone; D003094:Collagen