Comparative phylogeny of the ammonia monooxygenase subunit A and 16S rRNA genes of ammonia-oxidizing bacteria.
A fragment of the ammonia monooxygenase gene (amoA) from 31 strains of ammonia-oxidizing bacteria (AOB) was sequenced and analysed phylogenetically. The results were compared with the phylogeny of 16S rDNA from AOB. For most groups of AOB we found a high consistency between the phylogenetic trees based on the 16S rDNA and amoA sequences. Although it is not a phylogenetic marker, using the amoA as a probe when studying microbial diversity will probably reduce the amount of non-AOB detected, compared to using rDNA based probes. The data presented in this paper extend and improve the basis for application of amoA in studies of AOB in the environment.
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Laboratory of Microbial Gene Technology, Department of Chemistry and Biotechnology, Agricultural University of Norway, P.O. Box 5051, N-1432, As, Norway. agot.aakara@ikb.nlh.no
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D005798:Genes, Bacterial; D010088:Oxidoreductases / Q000145:classification* / Q000235:genetics; D010802:Phylogeny; D020560:Proteobacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D012329:RNA, Bacterial / Q000145:classification* / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000145:classification* / Q000235:genetics; D013045:Species Specificity
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C045582:ammonia monooxygenase