Implication of co-measured platelet factor 4 in the reliability of the results of the plasma transforming growth factor-beta 1 measurement.
We examined the possible alteration of circulating transforming growth factor-beta1 (TGF-beta1) concentrations in a time-dependent fashion in human plasma. Plasma TGF-beta1 was measured three times at 2 week-intervals from each of 12 healthy participants. Platelet factor 4 (PF4) was measured in parallel with TGF-beta1 to estimate the degree of platelet degranulation. TGF-beta1 levels of the second and third plasma samples, in which PF4s were measured as < approximately 1000 IU/ml, were relatively low and fell in a narrow range. However, TGF-beta1 levels of the first samples, in most of which PF4s were > approximately 1000 IU/ml, appeared much higher and more variable than those of the second or third samples. These results indicate that the platelet degranulation accounted for the higher TGF-beta1 levels in the first samples, and thus did not support our initial assumption. We, nevertheless, could propose a useful guidance in the assessment of TGF-beta1 levels in plasma. When the PF4 level is measured as < approximately 1000 IU/ml under our assay conditions, the TGF-beta1 level in a given plasma sample might be accepted as a reliable value considering the effect of platelet degranulation on TGF-beta1 level.
Jeon
J H
JH
Department of Neurobiology, Gyeongsang National University Graduate School, Chinju 660-702, Korea.
Kim
Y S
YS
Choi
E J
EJ
Cheon
S
S
Kim
S
S
Kim
J S
JS
Jang
J S
JS
Ha
W S
WS
Park
S T
ST
Park
C S
CS
Park
K
K
Park
B K
BK
D000328:Adult; D001792:Blood Platelets / Q000473:pathology*; D002624:Chemistry, Clinical / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010978:Platelet Factor 4 / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000097:blood*; D053773:Transforming Growth Factor beta1
C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D010978:Platelet Factor 4