Cytogenetic investigation of fetuses and infants conceived through intracytoplasmic sperm injection.
OBJECTIVE
To determine the incidence of aneuploidy among fetuses and infants conceived through intracytoplasmic sperm injection (ICSI) in our clinic using umbilical cord blood samples.


METHODS
Follow-up study of the cytogenetic outcome of ICSI pregnancies.


METHODS
University-based IVF clinic.


METHODS
Forty-six couples who underwent ICSI and conceived.


METHODS
Umbilical cord blood was taken after delivery of the infant for analysis. Samples of chorionic villi and chorion were taken for studies on the spontaneous abortuses. Amniocentesis was performed for couples that chose prenatal diagnosis.


METHODS
The cytogenetic chromosomal status of the pregnancy outcome.


RESULTS
Fifty pregnancies and 55 live births were recorded, with nine spontaneous abortions. Of 43 separate umbilical cord blood samples analyzed, 1 abnormality (2%) was found, 45, XX,+21. Nine births went through prenatal diagnosis alone, with four accepting both forms of analysis-no abnormalities were found. Origin of abnormality was established in two spontaneous abortion cases (45, XO and 45, XY,-21), and the maternal chromosome was lost in both cases.


CONCLUSIONS
Using umbilical cord blood obtained after birth, we obtained karyotype results from 78% of the ICSI population in our clinic. Combined with results from five additional cases that underwent prenatal diagnosis but not umbilical cord blood sampling, a chromosomal result was obtained in 87% of our ICSI population. The use of umbilical cord blood for cytogenetic analysis substantially improves the ability to determine rates of chromosomal abnormalities in newborns produced via ICSI clinics.
Lam
R
R
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver Hospital and Health Sciences Centre, Vancouver, British Columbia, Canada.
Ma
S
S
Robinson
W P
WP
Chan
T
T
Yuen
B H
BH
D000328:Adult; D000649:Amniocentesis; D000782:Aneuploidy; D005260:Female; D005312:Fetal Blood / Q000502:physiology; D005333:Fetus / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D020554:Sperm Injections, Intracytoplasmic