Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants.
A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.
Wang
J
J
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Ha
W Y
WY
Ngan
F N
FN
But
P P
PP
Shaw
P C
PC
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal; D008969:Molecular Sequence Data; D005894:Panax / Q000145:classification / Q000235:genetics*; D018517:Plant Roots; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D016415:Sequence Alignment
D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal