title: A catalytic subunit of the sugar beet protein kinase CK2 is induced by salt stress and increases NaCl tolerance in Saccharomyces cerevisiae.
abstract: Salinity is an important limiting factor in plant growth and development. We have cloned a catalytic subunit of the sugar beet protein kinase CK2 (BvCKA2) by functional expression in yeast of a NaCl-induced cDNA library. BvCKA2 was able to increase the yeast tolerance to NaCl and to functionally complement the cka1 cka2 yeast double mutant upon over-expression. Southern blot analysis indicated that, in sugar beet, the BCKA2 gene is a member of a multigene family. The mRNA levels of BvCKA2 were up-regulated in response to NaCl stress which suggests that protein kinase CK2 may be involved in the plant response to salt stress.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Kanhonou
authors.forename: R
authors.initials: R
authors.lastname: Serrano
authors.forename: R R
authors.initials: RR
authors.lastname: Palau
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D027461:Beta vulgaris / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D047390:Casein Kinase II; D020134:Catalytic Domain; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D011188:Potassium / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D012965:Sodium Chloride; D012964:Sodium; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D011188:Potassium