Responses of anthocyanin-producing and non-producing cells of Glehnia littoralis to radical generators.
The responses of anthocyanin-producing (violet) and non-producing (white) cells of Glehnia littoralis to radical generators were compared. Cell growth, anthocyanin content, phenylalanine ammonia-lyase (PAL) activity and furanocoumarin production were determined after treatment with H(2)O(2), 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), X-ray and yeast extract, independently. AAPH and H(2)O(2) repressed the growth of both violet and white cells, but violet cells grew better than white cells. On the other hand, the anthocyanin content in violet cells decreased. Neither X-ray nor yeast extract affected cell growth or pigment production. Treatment with H(2)O(2), yeast extract, and X-ray, but not AAPH, induced PAL activity and furanocoumarin production in white cell cultures, whereas violet cell cultures did not produce furanocoumarin following any of the treatment employed.
Kitamura
Yoshie
Y
School of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi 1-14, 852-8521, Nagasaki, Japan. k-yoshie@net.nagasaki-u.ac.jp
Ohta
Mina
M
Ikenaga
Toshihiko
T
Watanabe
Masami
M
D000578:Amidines / Q000494:pharmacology*; D000872:Anthocyanins / Q000378:metabolism* / Q000528:radiation effects; D019661:Apiaceae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D003374:Coumarins / Q000819:agonists / Q000378:metabolism / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010650:Phenylalanine Ammonia-Lyase / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014965:X-Rays
D000578:Amidines; D000872:Anthocyanins; D003374:Coumarins; C046728:2,2'-azobis(2-amidinopropane); C030123:coumarin; D006861:Hydrogen Peroxide; D010650:Phenylalanine Ammonia-Lyase