Vacuolar H+-ATPase, but not mitochondrial F1F0-ATPase, is required for aluminum resistance in Saccharomyces cerevisiae.
It was recently shown that vacuolar ATPase and mitochondrial F1F0-ATPase activities are induced by aluminum (Al) in an Al-resistant cultivar of wheat, suggesting that induction of these enzymes could be an adaptive trait involved in Al resistance. To test this hypothesis, we used the Saccharomyces cerevisiae model system. In yeast, unlike wheat, the activity, transcript and protein levels of mitochondrial F1F0-ATPase, but not vacuolar ATPase, are induced by Al, while plasma membrane P-ATPase activity is inhibited. However, yeast vacuolar ATPase mutant strains are hypersensitive to Al, while F1F0-ATPase mutant strains exhibit wild-type growth. These data suggest that vacuolar ATPase activity is involved in Al resistance, with ATP required for this activity supplied by mitochondrial F1F0-ATPase or fermentation.
Hamilton
C A
CA
Department of Biological Sciences, University of Alberta, T6G 2E9, Edmonton, AB, Canada. cah@ualbeta.ca
Good
A G
AG
Taylor
G J
GJ
D000535:Aluminum / Q000494:pharmacology*; D015152:Blotting, Northern; D004305:Dose-Response Relationship, Drug; D025141:Drug Resistance, Fungal; D015151:Immunoblotting; D025261:Mitochondrial Proton-Translocating ATPases / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D025262:Vacuolar Proton-Translocating ATPases / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D012331:RNA, Fungal; D012333:RNA, Messenger; D000535:Aluminum; D025262:Vacuolar Proton-Translocating ATPases; D025261:Mitochondrial Proton-Translocating ATPases