Phase transitions and hydrogen bonding in a bipolar phosphocholine evidenced by calorimetry and vibrational spectroscopy.
As a model for natural archaebacterial bolalipids, we have synthesized omega-hydroxybehenylphosphocholine (HBPC, HO-(CH(2))(22)-OP(O(-)(2))O-(CH(2))(2)-N+(CH(3))(3)) and investigated it, by Fourier-transform infrared and Raman spectroscopy and differential scanning calorimetry, both as fully hydrated dispersions (varying temperature) and as aligned films (varying hydration) in terms of particular structural features predestining such bipolar lipids for their occurrence in extremophilic organisms. The phase behavior of HBPC in dispersions depends on sample pretreatment as it comprises metastabilities in annealed samples. However, main transition proceeds consistently near 81 degrees C. Some (extra) deal of headgroup (phosphate) hydration accompanying a gel-gel phase transition near 66 degrees C appears to precede chain melting. Studies with HBPC films revealed lamellar interdigitated-like solid phases with an extraordinarily strong omega-OH--OPO(-) omega-OH--OPO(-) omega-OH hydrogen-bond pattern formed along both sides of the resulting monolayers. The "clamping" effect inherent to such structures provides a clue to explain the relatively high main-transition temperature of HBPC assemblies.
Pohle
W
W
Institute of Molecular Biology, Friedrich-Schiller University Jena, Winzerlaer Strasse 10, D-07745 Jena, Germany. wapo@molebio.uni-jena.de
Selle
C
C
Rettig
W
W
Heiser
U
U
Dobner
B
B
Wartewig
S
S
D000327:Adsorption; D002151:Calorimetry / Q000379:methods; D002152:Calorimetry, Differential Scanning; D006860:Hydrogen Bonding; D008055:Lipids / Q000737:chemistry; D008956:Models, Chemical; D010713:Phosphatidylcholines / Q000737:chemistry*; D010767:Phosphorylcholine / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000378:metabolism
D008055:Lipids; D010713:Phosphatidylcholines; C447039:omega-hydroxybehenylphosphocholine; D014867:Water; D010767:Phosphorylcholine