[Hydrophobic DNA adducts in relationship to estrogen and progesterone receptors content in human uterine cancer].
OBJECTIVE
Determination of the relationship between hydrophobic DNA adducts (A) and estrogen receptors (ER) and progesterone (PR) receptor status in uterine cancers.


METHODS
Using the P1 enriched version of 32P-postlabeling for hydrophobic DNA adducts detection on polyethyleneimine (PEI) cellulose thin layer chromatograms (TLC) we examined 11 uterine cancer DNAs. The quantification of the adducts was performed by Cerenkov counting of the spots. ER and PR status was recognized histochemically and H-score estimate was performed for each investigated cancer tissue. Patterns of uterine cancer DNA adducts were compared to the maps of adducts recognized in normal human endometrium.


RESULTS
In three of the studied uterine cancers there was no positive staining of ER and PR; in one case there was a weak ER staining but PR staining was negative. In ER negative tumors the A level was significantly higher than in ER positive cancers (138.1 +/- 64.1 vs. 49.7 +/- 26.8 adducts per 10(9) nucleotides, respectively, p < 0.05). Highest A levels were found in two ER and PR negative G3 metastatic tumors. Finally, in all investigated cancers there was a strong, inverse correlation between ER content and A level (r = -0.67, p < 0.03). In addition, the correlation between PR level and A was of borderline significance (r = -0.6, p = 0.053). The TLC patterns of adducts in uterine tumors were found to be qualitatively similar, but not quantitatively, to those observed in normal human endometrium DNA.


CONCLUSIONS
The data presented suggest that the hydrophobic DNA adducts could play a role in a sex-steroid hormone independence of human endometrial cancers. The highest accumulation of DNA adducts was recognized in neoplasms displaying the most malignant phenotype.
Postawski
K
K
Kliniki Ginekologii Operacyjnej II Katedry Połoznictwa i Chorób Kobiecych AM w Lublinie.
Olech-Fudali
E
E
Korobowicz
E
E
Jakowicki
J A
JA
Keith
G
G
Baranowski
W
W
D001345:Autoradiography; D018736:DNA Adducts / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology*
D018736:DNA Adducts; D004273:DNA, Neoplasm; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone