Effect of N-acetyl-D-glucosamine, and glycerol concentration and equilibration time on acrosome morphology and motility of frozen-thawed boar sperm.
A series of experiments were conducted to determine the effect of N-acetyl-D-glucosamine, glycerol concentration and equilibration time for the freezing of boar spermatozoa in 5 ml maxi-straws. The optimum final glycerol concentration in the diluent with 0.05% N-acetyl-D-glucosamine in the first diluent was 2-3% and the optimum glycerol equilibration time was 2-3h. In conclusion, we recommend the first diluent containing 11% lactose hydrate, 20% egg yolk and 0.05% N-acetyl-D-glucosamine in 100ml distilled water, and the second diluent containing 11% lactose hydrate, 20% egg yolk, 4% glycerol and 1% orvus es paste for the diluents of boar sperm freezing. Also, we found out that 0.05% soluble N-acetyl-D-glucosamine was the optimum concentration in the first diluent and a concentration of 0.05% soluble N-acetyl-D-glucosamine significantly enhanced the cryopreservation of boar spermatozoa.
Yi
Y J
YJ
Department of Animal Science, Chungnam National University, RK 305-764, Daejon, South Korea.
Cheon
Y M
YM
Park
C S
CS
D000117:Acetylglucosamine / Q000008:administration & dosage / Q000494:pharmacology*; D000177:Acrosome / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D005990:Glycerol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects; D013552:Swine; D013997:Time Factors
D003451:Cryoprotective Agents; D005990:Glycerol; D000117:Acetylglucosamine