Crystallization and preliminary crystallographic studies of dimeric disintegrins from the venom of two Agkistrodon snakes.
Piscivostatin (PVS) and acostatin are members of the disintegrin family of platelet-aggregation inhibitors found in snake venoms and are dimeric disintegrins which play different roles to the monomeric disintegrins. The crystals of PVS belonged to the P2(1)2(1)2(1) space group, with unit-cell parameters a = 34.3, b = 54.4, c = 115.9 A, and diffracted to 2.0 A resolution. The crystals of acostatin belonged to the P2(1) space group, with unit-cell parameters a = 55.4, b = 69.3, c = 63.5 A, beta = 111.7 degrees, and diffracted to 2.8 A resolution.
Fujii
Yoshifumi
Y
Department of Biochemistry, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Okuda
Daiju
D
Fujimoto
Zui
Z
Morita
Takashi
T
Mizuno
Hiroshi
H
D017836:Agkistrodon; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D019483:Disintegrins / Q000737:chemistry*; D004546:Elapid Venoms / Q000737:chemistry*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry*; D011487:Protein Conformation; D013045:Species Specificity
D019483:Disintegrins; D004546:Elapid Venoms; D010975:Platelet Aggregation Inhibitors; C451000:acostatin; C441662:piscivostatin