Role of Fas/FasL genes in azoospermia or oligozoospermia induced by testosterone undecanoate in rhesus monkey.
OBJECTIVE
To investigate role of apoptosis related genes Fas/FasL in azoospermia or oligozoospermia induced by testosterone undecanoate.


METHODS
TUNEL was used to detect the apoptotic signal of testicular cells. Immunohistochemistry and Western blot were used to quantitatively or qualitatively analyze the expression of these apoptosis-related proteins.


RESULTS
After injection of testosterone undecanoate, both the apoptotic signal in germ cells and the expression of Fas/FasL in testis increased correlatively in a time-dependent manner, reaching a maximum level on day 30.


CONCLUSIONS
Fas system may initiate and regulate the germ cell apoptosis induced by testosterone undecanoate.
Zhou
X C
XC
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Wei
P
P
Hu
Z Y
ZY
Gao
F
F
Zhou
R J
RJ
Liu
Y X
YX
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003272:Contraceptive Agents, Male; D053222:Fas Ligand Protein; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics / Q000502:physiology*; D009845:Oligospermia / Q000139:chemically induced* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor; D013094:Spermatozoa / Q000473:pathology; D013739:Testosterone / Q000031:analogs & derivatives*; D019014:fas Receptor
D003272:Contraceptive Agents, Male; C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D009479:Neuropeptides; D018124:Receptors, Tumor Necrosis Factor; D019014:fas Receptor; D013739:Testosterone; C010792:testosterone undecanoate