title: Effects of dental materials on insulin release from isolated islets of Langerhans.
abstract: OBJECTIVE
  To investigate the possibility of using a whole organ model for evaluating the biological effects of dental restoration materials in vitro.
  
  
  METHODS
  The effect on insulin release of isolated Langerhans islets of a series of dental materials was examined. The islets were incubated for 1h with extracts obtained from various dental materials and insulin was assayed radioimmunologically with crystalline mouse insulin. The results were analysed statistically using analysis of variance (ANOVA) and Scheffe's test at a significance level of p < 0.05.
  
  
  RESULTS
  One dental ceramic, Vita VMK 95, significantly (p < 0.01) decreased the insulin release, whereas another dental ceramic, Empress (p < 0.01), a partly re-cast high-noble gold alloy (p < 0.001), a modified high-noble gold alloy (p < 0,05), and unalloyed copper (p < 0.001) significantly increased the release of insulin.
  
  
  CONCLUSIONS
  The results demonstrate a new instance of examining the biological effects of dental restoration materials. The method provides information about the effect of different materials on organ level in vitro that can complement other in vitro tests.
authors.affiliation: Department of Integrative Medical Biology, Section for Histology and Cell Biology, Faculty of Medicine and Odontology, Umeå University, SE-901 87, Umeå, Sweden.
authors.forename: Solveig
authors.initials: S
authors.lastname: Persson-Sjögren
authors.forename: Göran
authors.initials: G
authors.lastname: Sjögren
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology; D000538:Aluminum Silicates / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology; D002858:Chromium Alloys / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003764:Dental Materials / Q000494:pharmacology*; D003776:Dental Porcelain / Q000494:pharmacology; D005260:Female; D006047:Gold Alloys / Q000494:pharmacology; D007328:Insulin / Q000032:analysis / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D016876:Metal Ceramic Alloys / Q000494:pharmacology; D051379:Mice; D008820:Mice, Obese; D013223:Statistics as Topic; D014025:Titanium / Q000494:pharmacology
chemical_list: D000538:Aluminum Silicates; D002858:Chromium Alloys; D003764:Dental Materials; C098572:Duceratin Porcelain; D006047:Gold Alloys; C075815:IPS-Empress ceramic; D007328:Insulin; D016876:Metal Ceramic Alloys; C080305:Vita VMK 595; C111324:vitadur alpha omega; D003776:Dental Porcelain; C058236:Vitadur N; D003300:Copper; D014025:Titanium; D000537:Aluminum Oxide