title: Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production.
abstract: Immune activation has been observed in HIV-infected and uninfected Africans, among whom it is thought to modify interaction between the immune system and HIV. To characterize this phenomenon accurately, in-depth immunologic analyses were performed in a rural African population. Freshly drawn peripheral blood mononuclear cells (PBMCs) of HIV-infected African (from Gulu, Uganda) and Italian antiviral-naive patients and those of uninfected Ugandan and Italian study subjects were analyzed. Individuals were matched for age and sex and determined to be free from parasitic infections. Intracellular cytokines were measured in mitogen (M)- and gp160 peptides + staphylococcal enterotoxin B and alpha CD28 (env)-stimulated T lymphocytes. Interferon (IFN)-gamma-producing CD8(+) T cells were quantified in an enzyme-linked immunosorbent assay. Results showed that M-stimulated production of interleukin (IL)-10 and tumor necrosis factor (TNF)-alpha increases in CD4(+) and CD8(+) cells of African infected patients and uninfected study subject; and that env-stimulated IL-10 and TNF-alpha production is increased in CD8(+) T lymphocytes of African HIV-infected patients. M- and env-stimulated IFN-gamma-producing CD8(+) T cells were reduced in African participants and not increased by preincubation with alpha IL-10 monoclonal antibody. This is the first set of data that has reported immune activation in rural Africa by single-cell analysis of cytokine production. These results help in defining the immunologic background to be considered in the design of therapeutic and vaccine-based approaches to HIV infection in an African setting.
authors.affiliation: St. Mary's Lacor Hospital, Gulu, Uganda.
authors.forename: M
authors.initials: M
authors.lastname: Lukwiya
authors.forename: G
authors.initials: G
authors.lastname: Rizzardini
authors.forename: D
authors.initials: D
authors.lastname: Trabattoni
authors.forename: S
authors.initials: S
authors.lastname: Piconi
authors.forename: M
authors.initials: M
authors.lastname: Saresella
authors.forename: S
authors.initials: S
authors.lastname: Declich
authors.forename: S
authors.initials: S
authors.lastname: Fossati
authors.forename: M
authors.initials: M
authors.lastname: Clerici
mesh_terms: D044383:Black People; D018106:CD28 Antigens / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000032:analysis*; D004768:Enterotoxins / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D019302:HIV Envelope Protein gp160 / Q000494:pharmacology; D015658:HIV Infections / Q000097:blood / Q000208:ethnology / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014454:Uganda / Q000208:ethnology
chemical_list: D018106:CD28 Antigens; D016207:Cytokines; D004768:Enterotoxins; D019302:HIV Envelope Protein gp160; D008934:Mitogens; C031818:enterotoxin B, staphylococcal