[Increase in thermosensitivity of cervical cancer and ovarian cancer cells by HSP70 antisense oligodeoxynucleotides].
OBJECTIVE
To investigate the thermosensitizing effect of HSP70 antisense oligodeoxynucleolides (ODN) on cervical and ovarian cancer cells.


METHODS
Cervical cancer cell line Hela and ovarian cancer cell line MCAS were used to study their sensitivity to heat (42 degrees C, 1 hr) treatment. Heat-induced changes in cell viability was assessed by colony formation assay and apoptosis by cell morphology, agarose gel electrophoresis and flow cytometry.


RESULTS
Thermosensitivity of Hela and MCAS cells pretreated with HSP70 antisense ODN was increased as compared to cells treated with heat alone. The increase was antisense dose dependent. Although heat treatment or antisense ODN did induce apoptosis of HeLa and MCAS cells, the percentage of apoptotic cells was significantly increased when both heat and antisense ODN were applied. The effect was dependent on the dose of HSP70 antisense ODN.


CONCLUSIONS
The thermosensitivity of ovarian cancer cells and cervial cancer cells can be increased by HSP70 antisense oligomer. Apoptosis may be involved in the increase in thermosensitivity.
Zhao
X
X
Department of Obstetrics and Gynecology, Second Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Wei
Y
Y
D017209:Apoptosis; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010051:Ovarian Neoplasms; D013696:Temperature; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms
D018840:HSP70 Heat-Shock Proteins; D020319:Oligodeoxyribonucleotides, Antisense