A glutathione S-transferase cDNA identified by mRNA differential display is upregulated during somatic embryogenesis in Cichorium.
CHI-GST1, a cDNA encoding a glutathione S-transferase, was isolated by differential display in leaf tissues of chicory, during the early stages of somatic embryogenesis. Expression analysis of the gene by Northern blot indicated that the transcript accumulation is specific of the leaf developing somatic embryogenesis and is not observed in leaf tissue of the non-embryogenic cultivar.
Galland
R
R
Laboratoire de Physiologie de la Différenciation Végétale, Université des Sciences et Technologies de Lille, Bâtiment SN2, 3e étage, 59655 Cedex, Villeneuve d'Ascq, France.
Randoux
B
B
Vasseur
J
J
Hilbert
J L
JL
D000595:Amino Acid Sequence; D018651:Cichorium intybus / Q000196:embryology* / Q000201:enzymology / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005838:Genotype; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D016415:Sequence Alignment; D015854:Up-Regulation
D018076:DNA, Complementary; D005982:Glutathione Transferase