[Detection of BCL2 translocation in an interphase neucleus using fluorescence in situ hybridization strategy].
OBJECTIVE
To establish a specific method to diagnose non-Hodgkin's lymphoma in clinic.


METHODS
Fluorescence in situ hybridization (FISH) strategy capable of detecting t(14;18)(q32;q21) chromosome translocation in an interphase nucleus was developed using a YAC clone containing the BCL2 gene and a phage clone containing IgHC(mu) region as probes. In the SU-DHL-6 cells, the translocated BCL2 allele (red) is overlapped by one of the green signals from IgH phage probe, while in normal lymphocytes the least distance of the hybridization signals from two different probes was either equal or larger than one-tenth of the diameter of a nucleus.


RESULTS
Based on the relative position of the signals from BCL2 and IgH probes the BCL2 translocation can be clearly detected in an interphase nucleus. The metaphase number and quality in samples, which could significantly restrict the reliability of cytogenetic analysis, can be ignored here.


CONCLUSIONS
This method shows a potentiality in clinical diagnosis of non-Hodgkin's lymphoma because of its objectivity, reliability and simplicity.
Sun
Y
Y
Division of Molecular Medicine in Beckman Research Institute, City of Hope National Medical Center, Duarte, California, CA91010 U.S.A. ysun@coh.org
Han
X
X
D002467:Cell Nucleus / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D019253:Proto-Oncogene Proteins c-bcl-2