[Differential display of vincristine-resistant proteins in gastric cancer cell line SGC7901].
OBJECTIVE
To find out new multidrug-resistant proteins in gastric cancer cells SGC7901 and to explain the new multidrug-resistant mechanism of gastric cancer cells.


METHODS
Two-dimensional gel electrophoresis was used with immobilized pH gradients (IPG) to compare the differential expression of multidrug-resistant proteins in gastric cancer cells SGC7901 and Vincristine-resistant SGC7901 cells (SGC7901/VCR) induced by vincristine sulfate. 2-D gels were used to silver stain the protein.


RESULTS
Approximately, 680 protein spots were identified in each of the 2-D gel patterns by silver stain. With most of them showing no difference in composition, shape or density, twenty-five proteins were found to differ in quantity (6 higher in SGC7901/VCR cells; 19 higher in 7901 cells). Five proteins were seen to be unique in one region or the others (3 in SGC7901/VCR cells, 2 in 7901 cells). The coordinate position of 30 protein spots in the 2-D gels were listed.


CONCLUSIONS
The results suggest that these differential proteins be related to the vincristine-resistant mechanism in human gastric cancer cell line SGC7901/VCR.
Wang
X
X
Institute of Digestive Diseases, PLA Fourth Military Medical University, Xi'an 710032, China.
Shi
Y
Y
Zhao
Y
Y
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D009363:Neoplasm Proteins; D014750:Vincristine