[Influence of fluid shear stress on the expression of monocyte chemotactic protein-1 by vascular endothelial cells].
OBJECTIVE
To study the effect of fluid shear stress on the expression of monocyte chemotactic protein-1(MCP-1) by vascular endothelial cells and its role in the early stages of atherogenesis(AS).


METHODS
Parallel plate flow chamber was used to expose vascular endothelial cells to different shear stress. Sandwich ELISA (enzyme-linked immunosorbent assay) and RT-PCR (reverse transcription polymerase chain reaction) were applied to detect MCP-1 protein and mRNA respectively.


RESULTS
Under 0.72 Pa shear stress, MCP-1 mRNA expression in endothelial cells reached a high level in 5 hours, but decreased to below the control level (P < 0.001) after 12 hours. The expression of MCP-1 mRNA showed a time dependent increase, which became slower 5 hours later. When different shear stress (0.30, 0.72, 2.40 Pa) was loaded for the same period of time (5 h), MCP-1 increased to above twice the control level, while its mRNA expression increased threefold as compared to the static control.


CONCLUSIONS
The expression of MCP-1 reacts strongly to fluid shear stress. The steady laminar flow down regulates the gene expression of MCP-1. These results may help to explain why AS lesions tends to occur at the site of turbulent blood flow.
Xing
H
H
Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. xing-hy@263.net
Li
H
H
Ma
L
L
Zhu
W
W
Jin
Y
Y
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D013314:Stress, Mechanical
D018932:Chemokine CCL2; D012333:RNA, Messenger