title: A preservation method that allows recovery of intact RNA from tissues dissected by laser capture microdissection.
abstract: We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis.
authors.affiliation: Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoles, Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Parlato
authors.forename: Annamaria
authors.initials: A
authors.lastname: Rosica
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cuccurullo
authors.forename: Luigi
authors.initials: L
authors.lastname: Mansi
authors.forename: Paolo
authors.initials: P
authors.lastname: Macchia
authors.forename: James D
authors.initials: JD
authors.lastname: Owens
authors.forename: J Frederic
authors.initials: JF
authors.lastname: Mushinski
authors.forename: Mario
authors.initials: M
authors.lastname: De Felice
authors.forename: Robert F
authors.initials: RF
authors.lastname: Bonner
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lauro
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015925:Cryopreservation / Q000379:methods; D004210:Dissection / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007834:Lasers; D051379:Mice; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D012333:RNA, Messenger; D013395:Sucrose; D012313:RNA