Short-term preservation of mouse oocytes at 5 degrees C.
The temporary preservation of oocytes without freezing would be useful for some experiments. ICR mouse oocytes were kept in a preservation medium under mineral oil for 1, 2, 3, 4 or 7 days at 5 degrees C, and 1 or 2 days at 37 degrees C. In vitro fertilization was attempted on oocytes rinsed with TYH medium after preservation. More than 70% of morphologically normal oocytes were recovered from each preservation group. Fertilization rates of oocytes preserved for 1, 2, 3, 4 or 7 days at 5 degrees C were 69.9, 66.5, 45.3, 26.7 and 8.8% respectively. Fertilization rates of oocytes preserved for 1 or 2 days at 37 degrees C were 9.6 and 1.6%, respectively. Preservation of oocytes at 5 degrees C has some capability as a method of short-term storage without freezing.
Tsuchiya
H
H
Tsukuba Primate Center, National Institute of Infectious Diseases, 1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan.
Ogonuki
N
N
Kuwana
T
T
Sankai
T
T
Kanayama
K
K
D000818:Animals; D005260:Female; D005306:Fertilization; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes; D013048:Specimen Handling; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*