title: Cryopreservation of gametes and embryos of non-domestic species.
abstract: Many species of mammals are threatened or endangered. Methods of assisted reproduction that are being used with increasing frequency to produce offspring of domestic animals and humans are often viewed as offering innovative ways to reproduce non-domestic species as well. Uncounted millions of live young of domestic or laboratory species have been produced from gametes and embryos stored at -70 degrees C or below, sometimes for as long as 25 to 35 yrs. Such methods of cryopreservation are now being applied with increasing frequency and urgency to preserve gametes and embryos of non-domestic and threatened species to establish "genome resource banks" or "frozen zoos." But levels of success to produce live young from such cryopreserved gametes or embryos vary considerably from species to species, as well as from individual to individual. It is sometimes thought that differences among species in fundamental characteristics of their gametes may determine the efficacy of cryopreservation and the production of live young. However, it may not be that ineffective cryopreservation is responsible for low success rates. Rather, the limiting factor may be insufficient information and knowledge of the most basic reproductive biology of such non-domestic species. Even standard methods of cryopreservation may be completely adequate to act as a "temporary" expedient to preserve germplasm of non-domestic species to permit time to acquire a fuller understanding of the biology and behavior of non-domestic species.
authors.affiliation: Audubon Nature Institute Center for Research of Endangered Species, Department of Biological Sciences, University of New Orleans, New Orleans, LA 70131, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Leibo
authors.forename: N
authors.initials: N
authors.lastname: Songsasen
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000196:embryology* / Q000235:genetics / Q000502:physiology*; D001188:Artiodactyla / Q000196:embryology / Q000235:genetics / Q000502:physiology; D002335:Carnivora / Q000196:embryology / Q000235:genetics / Q000502:physiology; D003247:Conservation of Natural Resources; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004606:Elephants / Q000196:embryology / Q000235:genetics / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D009865:Oocytes / Q000502:physiology*; D010527:Perissodactyla / Q000196:embryology / Q000235:genetics / Q000502:physiology; D011323:Primates / Q000196:embryology / Q000235:genetics / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*