Translocation of Cockayne syndrome group A protein to the nuclear matrix: possible relevance to transcription-coupled DNA repair.
Transcription-coupled repair (TCR) efficiently removes a variety of lesions from the transcribed strand of active genes. By allowing rapid resumption of RNA synthesis, the process is of major importance for cellular resistance to transcription-blocking genotoxic damage. Mutations in the Cockayne syndrome group A or B (CSA or CSB) gene result in defective TCR. However, the exact mechanism of TCR in mammalian cells remains to be elucidated. We found that CSA protein is rapidly translocated to the nuclear matrix after UV irradiation. The translocation of CSA was independent of Xeroderma pigmentosum group C, which is specific to the global genome repair subpathway of nucleotide excision repair (NER) and of the core NER factor Xeroderma pigmentosum group A but required the CSB protein. In UV-irradiated cells, CSA protein colocalized with the hyperphosphorylated form of RNA polymerase II, engaged in transcription elongation. The translocation of CSA was also induced by treatment of the cells with cisplatin or hydrogen peroxide, both of which produce damage that is subjected to TCR but not induced by treatment with dimethyl sulfate, which produces damage that is not subjected to TCR. The hydrogen peroxide-induced translocation of CSA was also CSB dependent. These findings establish a link between TCR and the nuclear matrix mediated by CSA.
Kamiuchi
Shinya
S
Division of Cellular Genetics, Institute for Molecular and Cellular Biology, Osaka University and Core Research for Evolutional Science and Technology (CREST), Japan.
Saijo
Masafumi
M
Citterio
Elisabetta
E
de Jager
Martijn
M
Hoeijmakers
Jan H J
JH
Tanaka
Kiyoji
K
D021581:Active Transport, Cell Nucleus; D000477:Alkylating Agents / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002945:Cisplatin / Q000494:pharmacology; D004260:DNA Repair; D045643:DNA Repair Enzymes; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009153:Mutagens / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010766:Phosphorylation; D021381:Protein Transport; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism; D013463:Sulfuric Acid Esters / Q000494:pharmacology; D013997:Time Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D014162:Transfection; D014466:Ultraviolet Rays
D000477:Alkylating Agents; C095472:ERCC8 protein, human; D009153:Mutagens; D016877:Oxidants; D011506:Proteins; D011838:Radiation-Sensitizing Agents; D013463:Sulfuric Acid Esters; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D045643:DNA Repair Enzymes; C007482:dimethyl sulfate; D002945:Cisplatin