title: Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis.
abstract: Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.
authors.affiliation: Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: Carrie
authors.initials: C
authors.lastname: Reed
authors.forename: Gregory D
authors.initials: GD
authors.lastname: Sturbaum
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hoover
authors.forename: Charles R
authors.initials: CR
authors.lastname: Sterling
mesh_terms: D000818:Animals; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D004275:DNA, Ribosomal / Q000032:analysis; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S