[Effect of Angelica sinensis injection on CD11c and CD14 expression in alveolar macrophage membrane of chronic bronchitis patients].
OBJECTIVE
To explore the effect of Angelica sinensis injection (ASI) in CD11c and CD14 expression in alveolar macrophage (AM) membrane of chronic bronchitis patients.


METHODS
AM from 10 chronic bronchitis patients (remmision stage) and 10 healthy subjects was obtained by bronchoalveolar lavage. After had cultured for 24 hours with ASI or/and lipopolysaccharide (LPS), CD11c and CD14 expression and intracellular calcium ion concentration in AM were analyzed by flow cytometry.


RESULTS
CD11c and CD14 expression levels of patients' AM membrane (n = 10) were (39.17 +/- 5.56)% and (35.73 +/- 8.05)% respectively, which were significantly higher than those of the control [n = 10, (16.29 +/- 3.78)% and (15.26 +/- 5.96)%], P < 0.05. LPS could increase the elevation of CD11c expression of patients [(47.25 +/- 7.00)%, P < 0.05], while ASI could reduce the increment [LPS Plus ASI group (27.61 +/- 8.64)%, P < 0.05]. The basic calcium level of AM cytoplasma of patients was (189.47 +/- 23.69) nmol/L (n = 7), which was higher than that of healthy control (99.65 +/- 32.21) nmol/L (n = 6), P < 0.05. The intracellular calcium ion elevation in AM of patients could be induced by LPS, and ASI could inhibit the elevation. The calcium level in LPS group was (288.47 +/- 43.68) nmol/L, in ASI Plus LPS group, (236.68 +/- 28.60) nmol/L, P < 0.05.


CONCLUSIONS
ASI could reduce the CD11c expression in AM of chronic bronchitis patients by inhibiting LPS induced intracellular calcium ion elevation in AM, suggesting that ASI may inhibit non-specific inflammation of respiratory tract.
Peng
Z
Z
Department of Pulmonary Diseases, Tongji Hospital of Tongji Medical University, Wuhan (430030).
Zhang
Z
Z
Xu
Y
Y
D000368:Aged; D029971:Angelica sinensis; D001991:Bronchitis / Q000188:drug therapy* / Q000276:immunology; D002908:Chronic Disease; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008517:Phytotherapy
D004365:Drugs, Chinese Herbal; D016167:Integrin alphaXbeta2; D018950:Lipopolysaccharide Receptors