F1F0-ATP synthase-stalking mind and imagination.
Electron microscopy together with image analysis has been used to study the structure of the intact F1F0-ATPsynthase from Escherichia coli. A procedure has been developed which allows preparation of detergent-free enzyme. Aside from the well known two-domain structure, images of F1F0 prepared by this procedure show a number of additional features, including a second stalk, which can be seen extending all the way from the F0 to the top of the F1 in some images, and a small protein on the very top of the F1, which has been identified as the delta subunit by decoration with a monoclonal antibody. In light of these results, a refined model of the subunit arrangement of the complex is presented.
Wilkens
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Department of Biochemistry, University of California, Riverside, 92521, USA. stephan.wilkens@ucr.edu
D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000201:enzymology*; D008854:Microscopy, Electron; D021122:Protein Subunits; D006180:Proton-Translocating ATPases / Q000648:ultrastructure*
D021122:Protein Subunits; D006180:Proton-Translocating ATPases