Fluorescence studies on PAMAM dendrimers interactions with bovine serum albumin.
Polyamidoamine (PAMAM) dendrimers (generation 3.5 and 4) interaction with bovine serum albumin (BSA) was studied. The intensity of intrinsic fluorescence of two tryptophan residues and a shift in wavelength of their emission maxima were chosen as indicators of protein conformational changes. It is shown that the generation 4 has a greater impact on spectral properties of serum albumin than generation 3.5.
Klajnert
B
B
Department of General Biophysics, University of Łódz, Banacha 12/16, 90-237 Łódz, Poland. aklajn@biol.uni.lodz.pl
Bryszewska
M
M
D050091:Dendrimers; D011073:Polyamines / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D050091:Dendrimers; C104700:PAMAM Starburst; D011073:Polyamines; D012710:Serum Albumin, Bovine