Effects of histamine and related compounds on the differentiation of HL-60-Eo cells into eosinophils.
The effects of histamine and related compounds on the differentiation of HL-60-Eo cells into eosinophils were studied. The histamine and H2 agonists impromidine and 4-methylhistamine caused concentration-related increases in the number of differentiated cells. On the other hand, the H1 agonists 2-methylhistamine and 2-pyndylethylamine showed no such effect. Histamine-induced eosinophil differentiation was antagonized by the H2 antagonists cimetidine and ranitidine. Histamine and H2 agonists inhibited (3H)-thymidine uptake, suggesting that these compounds caused a decrease in proliferation. Histamine as well as the H2 agonists impromidine and 4-methythistamine caused increases in cAMP level, and this effect was antagonized by ranitidine. From these findings, we concluded that both the differentiation of HL-60-Eo cells into eosinophils and proliferation of HL-60-Eo cells were mediated via H2 receptors.
Shimada
K
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Abe
T
T
Zhao
Q
Q
Mio
M
M
Kamei
C
C
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002927:Cimetidine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006632:Histamine / Q000031:analogs & derivatives / Q000494:pharmacology*; D017442:Histamine Agonists / Q000494:pharmacology; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D006801:Humans; D017446:Impromidine / Q000494:pharmacology; D008761:Methylhistamines / Q000378:metabolism / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D011970:Receptors, Histamine H2 / Q000378:metabolism; D013936:Thymidine / Q000493:pharmacokinetics; D013997:Time Factors; D014316:Tritium
D017442:Histamine Agonists; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D008761:Methylhistamines; D011970:Receptors, Histamine H2; D014316:Tritium; C025566:4-methylhistamine; D002927:Cimetidine; D006632:Histamine; D011899:Ranitidine; D017446:Impromidine; D000242:Cyclic AMP; C026259:2-methylhistamine; D013936:Thymidine