Essential role for the lymphostromal plasma membrane Ly-6 superfamily molecule thymic shared antigen 1 in development of the embryonic adrenal gland.
Thymic shared antigen 1 (TSA-1) is a plasma membrane protein of the Ly-6 superfamily expressed on thymocytes, thymic stromal cells, and other cells of the hematopoietic system. TSA-1 is also expressed in other nonhematopoietic tissues, in particular, embryonic and adult adrenal glands. To address the function of TSA-1, we generated mutant mice in which TSA-1 expression was inactivated by gene targeting. Here we show that deletion of both TSA-1 alleles results in abnormal adrenal gland development and midgestational lethality due to cardiac abnormalities. We also report that TSA-1-deficient adrenal glands have significantly reduced levels of the catecholamines noradrenaline and adrenaline. We conclude that TSA-1 is required for normal embryonic development but that deletion of its expression does not obviously impair lymphoid development.
Zammit
David J
DJ
Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia. david.zammit@med.monash.edu
Berzins
Stuart P
SP
Gill
Jason W
JW
Randle-Barrett
Elise S
ES
Barnett
Louise
L
Koentgen
Frank
F
Lambert
Gavin W
GW
Harvey
Richard P
RP
Boyd
Richard L
RL
Classon
Brendan J
BJ
D000311:Adrenal Glands / Q000002:abnormalities / Q000196:embryology* / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000950:Antigens, Ly / Q000235:genetics / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D004837:Epinephrine / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D006330:Heart Defects, Congenital / Q000196:embryology / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009638:Norepinephrine / Q000378:metabolism; D010641:Phenotype; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D000950:Antigens, Ly; D008565:Membrane Proteins; C084839:thymic shared antigen-1; D009638:Norepinephrine; D004837:Epinephrine