Peptide analog of fibronectin that inhibits cell migration and ERK 1/2 activity.
Two analogs of the peptide mimicking the 1977-1991 C- terminal part of fibronectin have been synthesized and tested. AWLI simulated human fibronectin fragment 1977-1991, whereas AWLII hybridized to both RGD and 1977-1991 fragments. AWLI and AWLII peptides inhibited the migration of the ovarian carcinoma cell line OVP10 regardless of the presence RGD. AWLI peptide inhibited spontaneous and fibronectin-activated cell migration and ERK1/2 activity. Neither AWLI nor fibronectin induced changes in FAK proteins, as could be judged from Western blots. In conclusion, it seems that the C-terminal fragment of fibronectin inhibits ERK1/2-dependent (random) migration of ovarian carcinoma cells.
Szaniawska
B
B
Department of Cell Biology, The Maria Sklodowska -Curie Memorial Cancer Center, WK Roentgen Str. 5, 02-781, Warsaw, Poland.
Trembacz
H
H
Miloszewska
J
J
Lipkowski
A W
AW
Misicka
A
A
Ostrowski
J
J
Janik
P
P
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D005353:Fibronectins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D009842:Oligopeptides; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases