[Effect of nm23H1 on proliferation, tumor formation and metastasis of hepatocarcinoma].
OBJECTIVE
To study the effects of nm23H1 on in vitro proliferation, in vivo tumor formation and metastasis of hepatocarcinoma.


METHODS
Vectors containing insert of either sense or antisense nm23H1 cDNA under the albumin enhancer/promoter for liver cell-specific expression were constructed. The expression vectors were transfected into hepatocarcinoma cell line SMMC-7721. Stable cell clones with maximum and minimum expression of nm23 were obtained. Cell growth was measured in vitro and metastasis was observed in vivo by injecting cells into nude mice, subcutaneously or under the spleen capsule.


RESULTS
In vitro, cell clone expressing antisense nm23H1 grew faster than that expressing sense nm23H1. In nude mice, the subcutaneously inoculated tumor grew faster and more aggressively in mice bearing antisense nm23H1 transfected hepatoma. So did metastasis of the antisense nm23H1 transfected hepatoma under the spleen capsule.


CONCLUSIONS
Apart from suppressing metastasis, nm23H1 significantly suppresses tumor cell proliferation in vitro and tumor growth in vivo.
Jin
S
S
Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200433, China.
Pan
X
X
Wang
Y
Y
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000473:pathology* / Q000556:secondary; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D015316:Genetic Therapy; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000517:prevention & control; D051379:Mice; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000627:therapeutic use*; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000517:prevention & control*; D009701:Nucleoside-Diphosphate Kinase; D014157:Transcription Factors / Q000235:genetics / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins