title: Histochemical detection of the lectin-binding carbohydrates in the zona pellucida during oocyte growth in the wild boar (Sus scrofa scrofa).
abstract: The changes that occur in the carbohydrate composition of zona pellucida glycoproteins during oocyte maturation in the wild-boar were studied using periodic-acid Schiff (PAS), High Iron Diamine (HID) and Low Iron Diamine (LID). Lectin staining was performed with a panel of 11 HRP-lectin conjugates combined with neuraminidase digestion and chemical treatments. There were few internal glucidic residues, such as N-acetylglucosamine, in the wild boar zona pellucida but there were many subterminal beta-N-acetylgalactosamine, alpha- and beta-galactose determinants masked by sialic acid. In addition, beta-N-acetylgalactosamine, beta-galactose-(1-3)-N-acetylgalactosamine and beta-galactose-(1-4)-N-acetylglucosamine were detected in the sulphated form in the terminal and/or subterminal position. Some differences in the lectin reactive sites occurred in the zona pellucida, depending on the stage of oocyte maturation.
authors.affiliation: Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, Perugia University, Italy. silvana@unipg.it
authors.forename: F
authors.initials: F
authors.lastname: Parillo
authors.forename: S
authors.initials: S
authors.lastname: Diverio
authors.forename: L
authors.initials: L
authors.lastname: Todini
authors.forename: O
authors.initials: O
authors.lastname: Fagioli
mesh_terms: D000818:Animals; D000835:Animals, Wild; D003959:Diamines; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006651:Histocytochemistry / Q000662:veterinary; D007501:Iron / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D009865:Oocytes; D010503:Periodic Acid-Schiff Reaction / Q000662:veterinary; D013552:Swine / Q000502:physiology*; D015044:Zona Pellucida / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003959:Diamines; D006023:Glycoproteins; D037102:Lectins; D009844:Oligosaccharides; D007501:Iron