title: The binding mode of human nucleoside diphosphate kinase B to single-strand DNA.
abstract: In this paper, we studied the interaction of the human isoform B of nucleoside diphosphate kinase (NDP kinase B) with the nuclease hypersensitive element (NHE) present in the promoter element of the c-myc oncogene. The DNA-binding properties of NDP kinase B and other NDP kinases are compared and the nucleotide requirement for binding are discussed. Using quantitative methods, we identified the DNA-binding sites on the protein and we proposed a structural model for a complex of one hexameric NDP kinase B with an oligonucleotide.
authors.affiliation: Institut Pasteur, Unité de Régulation Enzymatique des Activités Cellulaires, CNRS URA 1773, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Agou
authors.forename: S
authors.initials: S
authors.lastname: Raveh
authors.forename: M
authors.initials: M
authors.lastname: Véron
mesh_terms: D004277:DNA, Single-Stranded / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D007527:Isoenzymes; D009838:Oligodeoxyribonucleotides; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D009701:Nucleoside-Diphosphate Kinase