[A qualititative study on p16 mutiple tumor suppressor gene, ras oncogene p21 and DNA content in parotid tumor and its' contiguous acini].
OBJECTIVE
To determine the biologic characteristics of tumor's contiguous acini and the relationship of recurrence or malignant transformation.


METHODS
With the use of immunohistochemistry and flow cytometry, the features of p16, p21 and DNA expression in 68 tumors and their contiguous acini were studied.


RESULTS
The expression of p16 and p21 gene were found in tumors that the possitive unit (PU) was higher than normal acini (P < 0.01). The areas of expression were mainly located in cytoplasm of gland duct cells. Strong possitives or weak possitives could be observed in the tissues of malignant tumors and their contiguous acini respectively, as well as the PU in the deep lobe of recurrent parotid tumors in which the capsule was incomplete was higher than that in the superficial lobe of primary parotid tumors with complete capsule (P < 0.05). Futhermoer the levels of DI, S% and PI were higher in perotid tumors and its contiguous acini than normal acini (P < 0.05).


CONCLUSIONS
When p16 multiple tumor suppressor gene is lost and oncogene p21 is mutated, the tumor's contiguous acini maybe in the condition of "paraneoplasm" after parotid tumor removal, and it has a strong ability of preliferation, which play an important role in recurrent or malignant change of the parotid tumor.
Zhu
S
S
Department of Stomatology, Tongji Hospital Tongji Medical University, Wuhan 430030, China.
Wang
X
X
Shao
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000235:genetics / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)