Difference in subcellular distribution between 45- and 37-kDa mevalonate pyrophosphate decarboxylase in rat liver.
We previously reported that the CP diet (a diet containing 5% cholestyramine and 0.1% pravastatin)-induced new species of 37-kDa mevalonate pyrophosphate decarboxylase (MPD) was characteristically and immunologically very similar to the well-known 45-kDa MPD. In the present study, we found a difference in subcellular distribution between 45- and 37-kDa MPD by cell fractionation and immunoblot analysis. The cytosol fraction contained 45- and 37-kDa MPD. Peroxisomal fraction contained a small amount of 45-kDa MPD, but not 37-kDa MPD. Also, 45-kDa MPD in peroxisome is localized in the matrix. From these data, the difference in subcellular distribution between 45- and 37-kDa MPD may be due to differences in the physiological role of cholesterol biosynthesis in rat liver.
Michihara
A
A
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. mitihara@fupharm.fukuyama-u.ac.jp
Sawamura
M
M
Yamori
Y
Y
Akasaki
K
K
Tsuji
H
H
D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis* / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000201:enzymology*; D008297:Male; D020675:Peroxisomes / Q000737:chemistry / Q000201:enzymology; D051381:Rats; D011921:Rats, Inbred WKY; D013347:Subcellular Fractions / Q000737:chemistry / Q000201:enzymology
D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase