[The expression and regulation of heme oxygenase-1 in asthmatic guinea pigs].
OBJECTIVE
To explore the expression and pathogenic mechanism of heme oxygenase-1(HO-1) in asthma.


METHODS
Two groups of guinea pigs (10 in each) were treated with the specific stimulator(hemin) and inhibitor(Sn-PP) of HO-1 respectively. Their effects on the levels of HO-1 activity, COHb, cGMP and IgE in serum or blood and lung tissues were compared with each other. Acute asthmatic group (AAG), dexamethasone-prevented group(DPG), stable asthmatic group, ovalbumin-sensitized group and normal control group(NCG) were also included. Lung tissues of tested animals were pathologically observed and immunohistochemically stained.


RESULTS
In the AAG and hemin stimulated group, the levels of COHb, cGMP and IgE were significantly increased with higher levels of activity and expression of HO-1 protein compared with the NCG (t = 4.575-10.188, P < 0.01). In DPG and Sn-PP inhibited groups, however, all measured parameters were markedly reduced compared with the AAG(P < 0.01). The other groups were normal.


CONCLUSIONS
The increased production and activity of HO-1 resulted in increases of endogenous CO and cGMP and promoted inflammation and immunoreaction in asthma.
Wang
H
H
Department of Pediatrics, Air Force 463 Hospital, Shenyang 110042.
Li
H
H
Pan
J
J
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics; D002248:Carbon Monoxide / Q000378:metabolism; D006152:Cyclic GMP; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic; D006168:Guinea Pigs; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D051547:Heme Oxygenase-1; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism
D009569:Nitric Oxide; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D006152:Cyclic GMP