[Stress response of Nilaparvata lugens at high temperature and activities of its protective enzyme systems].
The nymphae and different wing-form adults of Brown Planthopper (BPH) were treated intermittently at 34 degrees C, 36 degrees C and 38 degrees C. During the process of heat shock treatment, the variation of the activities of the endogenous enzymes of protective system and the content of lipid peroxides (LPO) in BPH were researched. The results showed that the effect of heat shock treatments on CAT activity in 4th instar of nymphae was higher than that in 3rd and 5th instar. The CAT activity in BPH adults increased with the day-age, and 36 degrees C was the suitable temperature at which H2O2 was scavenged by CAT. The GSH-px activity was higher in old than that in young nymphae, and the ability of GSH-px to scavenge H2O2 was higher in old adults than in those adults emerged early. The activity of SOD had a positive correlation with the treated temperatures, and decreased with the increase of nymphae instar and the day-age of adults after emergence at the same temperature. The activities of CAT, GSH-px, and SOD in macropterous adults were higher than those in brachypterous adults. There were no significant difference for the activities of CAT, GSH-px and SOD between female and male adults (alpha > 0.05). The content of LPO in BPH increased with the rise of treated temperature, and also increased with the increase of instars in nymphae and day-age after emergence in adults at the same temperature. The LPO content was higher in brachypterous than in macropterous adults.
Feng
C
C
Department of Plant Protection, Nanjing Agricultural University, Nanjing 210095. cj-Feng@990.net
Dai
H
H
Wu
S
S
D000818:Animals; D018869:Heat-Shock Response / Q000502:physiology*; D006430:Hemiptera / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D008054:Lipid Peroxides / Q000378:metabolism; D013696:Temperature
D008054:Lipid Peroxides