Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay.
The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4 degrees C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories.
Riddell
Michaela A
MA
Victorian Infectious Diseases Reference Laboratory/WHO Western Pacific Measles Regional Reference Laboratory, North Melbourne, Australia. michaela.riddell@mh.org.au
Leydon
Jennie A
JA
Catton
Mike G
MG
Kelly
Heath A
HA
D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D001800:Blood Specimen Collection / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007075:Immunoglobulin M / Q000097:blood*; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014680:Veins
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic