Increased activity of c-Src and Csk in fibroblasts transformed by v-src oncogene.
When c-Src and v-Src were immunoprecipitated together from hamster fibroblasts transformed by Rous sarcoma virus containing v-src oncogene, the total Src activity was almost threefold higher compared to c-Src activity in the control cells. The activity of v-Src immunoprecipitated separately, however, accounting for only 40% of the total Src activity, indicating that c-Src is activated upon transformation. An increased activity of Csk was also found in RSV-transformed cells. It decreased upon serum stimulation in parallel with an increase in Src kinase activity. In nontransformed cells, serum stimulation induced an enhanced Csk activity, but no changes in c-Src activity were observed. This may suggest that Csk may have more functions in hamster fibroblasts, in addition to its inhibitory effect on c-Src.
Tuhácková
Zdena
Z
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague 6, 166 37, Czech Republic. tuhack@img.cas.cz
Vojtechová
Martina
M
Hlavácek
Jan
J
Ruzzene
Maria
M
Sovová
Vlasta
V
Pinna
Lorenzo A
LA
D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001358:Avian Sarcoma Viruses / Q000235:genetics; D001798:Blood Proteins / Q000494:pharmacology; D000081247:CSK Tyrosine-Protein Kinase; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000502:physiology*; D006224:Cricetinae; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D020123:Sirolimus / Q000494:pharmacology; D000077191:Wortmannin; D019061:src-Family Kinases
D000730:Androstadienes; D000903:Antibiotics, Antineoplastic; D001798:Blood Proteins; D004791:Enzyme Inhibitors; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D015688:Oncogene Protein pp60(v-src); D016392:Proto-Oncogene Proteins pp60(c-src); D019061:src-Family Kinases; D020123:Sirolimus; D000077191:Wortmannin