The avian telencephalic subpallium originates inhibitory neurons that invade tangentially the pallium (dorsal ventricular ridge and cortical areas).
Recent data on the development of the mammalian neocortex support that the majority of its inhibitory GABAergic interneurons originate within the subpallium (ganglionic eminences). Support for such tangential migration into the pallium has come from experiments using fluorescent tracers or lineage analysis with retrovirus, and the phenotypes of mutant mice with different abnormalities in the developing subpallium. In the present study, we describe tangential migration of subpallial-derived neurons in the developing chick telencephalon. Using quail-chick grafts, we precisely identified the neuroepithelial origin, time-course, and pathways of migration, as well as the identity and relative distribution of the diverse tangentially migrated neurons. The analysis of selective grafts of the pallidal and striatal primordia allowed us to determine the relative contribution of each primordium to the population of migrating neurons. Moreover, we found that, like in mammals, the vast majority of the GABAergic and calbindin-immunoreactive neurons within the pallium (dorsal ventricular ridge and cortical areas) have an extracortical, subpallial origin. Our results suggest that the telencephalon of birds and mammals share developmental mechanisms for the origin and migration of their cortical interneurons, which probably first evolved at an earlier stage in the radiation of vertebrates than was thought before.
Cobos
I
I
Department of Morphological Sciences, University of Murcia, Murcia 30100, Spain.
Puelles
L
L
Martínez
S
S
D000818:Animals; D016380:Brain Tissue Transplantation; D064026:Calbindins; D002452:Cell Count; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002470:Cell Survival; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000637:transplantation; D002642:Chick Embryo; D004847:Epithelial Cells / Q000166:cytology; D007120:Immunochemistry; D007395:Interneurons / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D010641:Phenotype; D011784:Quail / Q000196:embryology*; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D013687:Telencephalon / Q000166:cytology* / Q000196:embryology* / Q000637:transplantation; D013997:Time Factors; D018183:Transplantation Chimera / Q000196:embryology; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid