New immunochromatographic method for rapid detection of rotaviruses in stool samples compared with standard enzyme immunoassay and latex agglutination techniques.
Three different commercial immunologic tests for rapid detection of group A rotavirus (an immunochromatographic method, latex agglutination, and enzyme immunoassay) were used to evaluate 228 faecal specimens obtained from Spanish children with acute gastroenteritis. After resolution of 30 (13.2%) discordant results by reverse transcription-polymerase chain reaction for rotavirus, the statistical values of the enzyme immunoassay, latex agglutination, and immunochromatographic method were respectively 96%, 68%, and 99% for sensitivity; 99%, 99%, and 96% for specificity; 98%, 96%, and 92% for positive predictive value; and 98%, 88%, and 99% for negative predictive value. The immunochromatographic technique showed high sensitivity and specificity and was rapid and easy to perform in the routine clinical laboratory.
Wilhelmi
I
I
Servicio de Microbiología, Hospital Severo Ochoa, Madrid, Spain.
Colomina
J
J
Martín-Rodrigo
D
D
Roman
E
E
Sánchez-Fauquier
A
A
D002675:Child, Preschool; D002845:Chromatography / Q000379:methods; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007841:Latex Fixation Tests; D008297:Male; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity