title: Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy.
abstract: Several attempts have been made to improve osseointegration of titanium alloy as an implant material by modification of its surface. In the present study, proliferation, differentiation, and mineralization of osteoblasts on type I collagen-coated Ti6Al4V were investigated. The activity of alkaline phosphatase and the accumulation of calcium by osteoblasts grown on titanium alloy were significantly higher compared to cells grown on polystyrene. Precoating of the implant surface with type I collagen did not extensively affect proliferation, the activity of alkaline phosphatase, collagen synthesis, calcium accumulation, or the mRNA levels for collagen I alpha1, osteopontin, osteocalcin, MMP-2, and TIMP-2. Maximum collagen synthesis by osteoblasts was observed at day 4 of culture independent of the type of implant material. The specific activity of alkaline phosphatase reached its maximum at day 18 of culture. Accumulation of calcium and elevated mRNA levels for osteocalcin were found at day 22. These results indicate that collagen-coating alone is not sufficient to accelerate differentiation of rat calvarial osteoblasts on Ti6Al4V.
authors.affiliation: Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Dresden University of Technology, Fiedlerstr. 42, D-01307 Dresden, Germany. dorit.becker@mailbox.tu-dresden.de
authors.forename: D
authors.initials: D
authors.lastname: Becker
authors.forename: U
authors.initials: U
authors.lastname: Geissler
authors.forename: U
authors.initials: U
authors.lastname: Hempel
authors.forename: S
authors.initials: S
authors.lastname: Bierbaum
authors.forename: D
authors.initials: D
authors.lastname: Scharnweber
authors.forename: H
authors.initials: H
authors.lastname: Worch
authors.forename: K-W
authors.initials: KW
authors.lastname: Wenzel
mesh_terms: D000497:Alloys; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D024042:Collagen Type I / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D012886:Skull / Q000166:cytology; D014025:Titanium
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; C031462:titanium alloy (TiAl6V4); D014025:Titanium