Neurotrophin and GDNF expression increases in rat adrenal glands during experimental colitis.
OBJECTIVE
Neurotrophins and GDNF have been recently recognized as important local regulators of inflammatory processes of the gut.


RESULTS
We now demonstrate that experimental TNBS-colitis is associated with the increased expression of neurotrophins and GDNF in the adrenal glands. In histological sections of the adrenals from untreated control animals, faint immunolabeling for BDNF, NT-3 and NGF was detectable in the adrenal cortex, with some additional labeling for NGF over the adrenal medulla, whereas GDNF immunolabeling was confined to the adrenal medulla. Induction of TNBS-colitis markedly increased NGF, BDNF, and NT-3 expression within the adrenal cortex after 8 h. NGF declined to basal levels after 7 days. In case of BDNF and NT-3 basal expression levels were reached after 14 days. GDNF expression was robustly upregulated in the adrenal medulla 8 h after induction of colitis and stayed elevated for up to 14 days.


CONCLUSIONS
Together these observations suggest that neurotrophins and GDNF might act as local modulators of components of the HPA-axis during peripheral inflammation.
Flämig
G
G
Department of Medicine I, University of Ulm, Germany.
Engele
J
J
Geerling
I
I
Pezeshki
G
G
Adler
G
G
Reinshagen
M
M
D000311:Adrenal Glands / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000235:genetics; D019439:Chromaffin Cells / Q000502:physiology; D003092:Colitis / Q000276:immunology* / Q000503:physiopathology*; D015870:Gene Expression / Q000276:immunology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008297:Male; D020932:Nerve Growth Factor / Q000032:analysis / Q000235:genetics; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics; D015213:Neuroimmunomodulation / Q000502:physiology; D020933:Neurotrophin 3 / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D019208:Brain-Derived Neurotrophic Factor; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D012333:RNA, Messenger; D020932:Nerve Growth Factor