title: [Correction of polypronuclear zygotes in 31 cases by micromanipulation].
abstract: OBJECTIVE
  To study the development of polypronuclear zygotes corrected by micromanipulation.
  
  
  METHODS
  Thirty one cases of polypronuclear zygotes were corrected during September to December of 1999 through micromanipulation to remove supernumerary pronucleus from polypronuclear zygotes at 37 degrees C and observing their development 24 and 48 hours later to compare with the normal pre-embryos.
  
  
  RESULTS
  17 in 31 (55%) cases of corrected polypronuclear zygotes developed to 2 to 4-cell pre-embryoes 24 hours later. There was significant difference while compare with the normal zygotes. However 48 hours later the cleavage rate of the two group of preembryos was the similar.
  
  
  CONCLUSIONS
  The correction of polypronuclear zygotes by micromanipulation may in some way cause damage to the cleavage ability in part of the zegotes, but the remaining can develope normally.
authors.affiliation: Department of Gynecology and Obstetrics, Nanfang Hospital, Guangzhou 510515, China.
authors.forename: L
authors.initials: L
authors.lastname: Kong
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: F
authors.initials: F
authors.lastname: Xing
mesh_terms: D002455:Cell Division; D002467:Cell Nucleus; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D012099:Reproductive Techniques; D015053:Zygote / Q000166:cytology*