[The coordinated expression of laminin and its integrin receptor in hepatic sinusoidal capillarization].
OBJECTIVE
To explore the expression of laminin and its integrin receptor alpha 6 in sinusoidal capillaration.


METHODS
The rat hepatic fibrosis model was established by injection carbon tetrachloride subcutaneously. Liver sections were examined by scanning electron microscopy and special staining. Then expressions of laminin and integrin alpha 6 subunit were observed by immunohistochemistry and dot immuno-blotting.


RESULTS
The pathologic character of hepatic fibrosis-sinusoidal capillaration was observed. Sinusoidal capillaration is characterized by defenestration of sinusoidal endothelial cells (SEC) and basement membrane forming and phenotype transformation. By immunohistochemistry staining. The deposition of laminin surrounding sinusoids according to fibrosis stage was 0, 1.92%, 6.02%, 9.68%, 14.14% respectively, statistically different from each other (P < 0.05). In normal rat no expression of integrin alpha 6 were observed in SEC membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoidal in accordance with laminin assembly. The content of integrin alpha 6 in fibrotic liver tissues were significantly higher than in normal liver tissues as measured by dot immuno-blotting (P < 0.05).


CONCLUSIONS
During fibrogenesis laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, meanwhile are company with induced expression of LN-binding-integrin alpha 6 on SEC membranes. These results suggest that coordinated expression of laminin and laminin-binding-integrin might be important in pathogenesis of sinusoidal capillaration and hepatic fibrosis.
Xiao
W
W
Department of Internal Medicine, First Hospital, West China University of Medical Sciences, Chengduo 610041, China.
Wang
Y
Y
Liu
X
X
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D039503:Integrin alpha6; D007797:Laminin / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D015703:Antigens, CD; D039503:Integrin alpha6; D007797:Laminin; D002251:Carbon Tetrachloride