Efficacy of cetylpyridinium chloride in immersion treatment for reducing populations of pathogenic bacteria on fresh-cut vegetables.
The efficacy of cetylpyridinium chloride (CPC) immersion to reduce the numbers of three pathogenic bacteria (Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7) on three different fresh-cut vegetables (broccoli, cauliflower, and radishes) was studied. The fresh-cut vegetables were inoculated with one of the three pathogenic bacteria at a concentration of 10(5) CFU/ml for 1 h at room temperature and then treated with 0.1 or 0.5% CPC immersion for 1 min. Both Salmonella Typhimurium and E. coli O157:H7 plates were incubated from 48 to 72 h at 37 degrees C, and L. monocytogenes plates were incubated from 72 to 96 h before being counted. The results of three experiments showed that for the average of the three vegetables treated with 0.1 and 0.5% CPC, L. monocytogenes was reduced by 2.85 and 3.70 log CFU/g, Salmonella Typhimurium by 2.37 and 3.15 log CFU/g, and E. coli O157:H7 by 1.01 and 1.56 log CFU/g, respectively, in comparison with the vegetables treated with water only. The 0.5% CPC treatment was significantly different (P < 0.05) from the 0.1% CPC treatment on reduction of L. monocytogenes, Salmonella Typhimurium, and E. coli O157:H7. The CPC residual on the treated vegetables and their washing solutions were evaluated by using high-performance liquid chromatography.
Wang
H
H
Poultry Science Department, University of Arkansas, Fayetteville 72701, USA.
Li
Y
Y
Slavik
M F
MF
D002594:Cetylpyridinium / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D004350:Drug Residues / Q000032:analysis; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D016896:Treatment Outcome; D014675:Vegetables / Q000382:microbiology*
D002594:Cetylpyridinium