CEPH family 1362 STR database: an online resource for characterization of PCR products using electrospray ionization mass spectrometry.
An online database has been established in order to validate electrospray ionization mass spectrometry (ESI-MS) for genotyping and to publicize the procedures developed in our laboratory for the characterization of PCR products by ESI-MS. Genotypes derived from short tandem repeat (STR) loci that were obtained using ESI Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) have been posted for fifteen members of the CEPH family 1362 pedigree. The website provides specific information such as PCR parameters, PCR product cleanup approaches, and ESI solution compositions to enable other laboratories to reproduce our data. Links are provided to related websites in an effort to integrate information regarding the CEPH family, STR genotyping, and mass spectrometry. The database, currently available at http://www.people.vcu.edu/ -dcmuddim/genotype/ will be routinely updated with genotypes from additional STR loci including PCR parameters as well as PCR cleanup strategies as further developments are completed.
Null
Allison P
AP
Department of Chemistry, Virginia Commonwealth University, Richmond, 23284, USA.
Muddima
David C
DC
D018076:DNA, Complementary / Q000737:chemistry*; D030541:Databases, Genetic; D005819:Genetic Markers; D006801:Humans; D020407:Internet; D016133:Polymerase Chain Reaction / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D020080:Tandem Repeat Sequences / Q000235:genetics*
D018076:DNA, Complementary; D005819:Genetic Markers