title: Cellular screening assays using fluorescence microscopy.
abstract: The recent development of automated fluorescence imaging systems has enabled fluorescence microscopy to be used for the purposes of compound screening. This information-rich technique has found various applications, including screening for the effect of kinase inhibitors on the cytoskeleton, agonist-stimulated receptor internalization, and protein phosphorylation and acetylation. The discovery of novel fluorescent proteins and new fluorescent dyes will find many applications in multichannel fluorescence imaging assays.
authors.affiliation: Sugen Inc., South San Francisco, CA 94080-4811, USA. robert-blake@sugen.com
authors.forename: R A
authors.initials: RA
authors.lastname: Blake
mesh_terms: D000818:Animals; D002477:Cells / Q000737:chemistry* / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes