Electrode potential-controlled DNA damage in the presence of copper ions and their complexes.
Supercoiled (sc) DNA immobilized at the surface of a hanging mercury drop electrode was cleaved by reactive oxygen species generated by an electrochemically modulated reaction of copper ions, hydrogen peroxide and/or oxygen. The cleavage was observed in a certain potential region where redox cycling of DNA-bound Cu(II)/Cu(I) took place. In the presence of 1,10-phenanthroline the maximum efficiency of DNA cleavage was shifted to more negative potentials and the effect was enhanced.
Fojta
Miroslav
M
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, CZ-612 65 Brno, Czech Republic. fojta@ibp.cz
Havran
Ludĕk
L
Kubicárová
Tatiana
T
Palecek
Emil
E
D002412:Cations; D003300:Copper / Q000737:chemistry*; D004249:DNA Damage; D004566:Electrodes; D010618:Phenanthrolines / Q000737:chemistry
D002412:Cations; D010618:Phenanthrolines; D003300:Copper; C025205:1,10-phenanthroline