[Radioimmunodetection of human trophoblastic cancer xenograft in nude mice].
OBJECTIVE
To study the efficiency of radioimmunodetection to locate xenograft of human trophoblastic cancer in nude mouse.


METHODS
Radioimmunodetection was performed with a cocktail of 131I-labeled mouse anti-hCG monoclonal antibodies to image xenogaft of human trophoblastic cancer in nude mice. Normal mouse IgG used to treat mice bearing trophoblastic cancer xenograft was used as control. Radioactivity in different tissues was measured and the tumor/non-tumor(T/NT) ratio was calculated.


RESULTS
The accumulation of radioactivity in the xenograft could be recognized as early as 24 hours after the injection of the radiolabeled anti-hCG antibodies. Radioactivity accumulation became increasingly evident with time. At 72-96 hours after injection of the radiolabled antibodies, the xenograft could be clearly shown. The minimal size of the xenograft with demonstrable radioactively was 0.8 cm in diameter. The T/NT ratio increased with time and was obviously higher than that in mice treated with normal mouse IgG.


CONCLUSIONS
Radioimmunodetection can efficiently locate human trophoblastic cancer xenograft in nude mice.
Wan
X
X
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730.
Zhang
D
D
Yang
X
X
D000818:Animals; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011247:Pregnancy; D016719:Radioimmunodetection; D014183:Transplantation, Heterologous; D014328:Trophoblastic Neoplasms / Q000000981:diagnostic imaging*; D014594:Uterine Neoplasms / Q000000981:diagnostic imaging*