[Effects of hexamethylene bisacetamide on in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1].
OBJECTIVE
To investigate the effects of hexamethylene bisacetamide (HMBA) on the in vitro adhesion, motility and invasion of human mucoepidermoid carcinoma cell line MEC-1.


METHODS
MEC-1 cells were induced with 2 mmol/L of HMBA in vitro, the adhesion of MEC-1 cells to the reconstituted basement membrane Matrigel was investigated with MTT colorimetric assay; the motility and invasion of MEC-1 cells were assayed using biopore membrane Millicell-PCF inserts culture system.


RESULTS
The adhesion of MEC-1 cells treated with HMBA for 30 min or 90 min reduced by 26.47% and 31.13%, respectively; the motility and invasion of MEC-1 cells treated with HMBA reduced by 63.49% and 67.65%, respectively.


CONCLUSIONS
HMBA has anti-invasion effects on human mucoepidermoid carcinoma cells in vitro.
Liu
B
B
Department of Biology, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Wu
J
J
Situ
Z
Z
D000081:Acetamides / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D018277:Carcinoma, Mucoepidermoid / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000081:Acetamides; D000970:Antineoplastic Agents; C014026:hexamethylene bisacetamide