title: Effect of a soluble ionic polymer on the separation of anions by capillary electrophoresis.
abstract: A silica capillary for CE analysis of anions can be conditioned with NaOH, rinsed with water, coated with a cationic polymer, and equilibrated with the background electrolyte in only 2 min for each of the four steps. The coated surface has a positive charge that gives a substantial anodic electroosmotic flow (EOF) over the range of pH 2.5-12.0. The migration times of sample anions and a neutral marker (used for EOF calculations) are generally reproducible to an RSD of 1% or better, both for successive runs on a single capillary and from capillary to capillary. It was shown that the type of buffer used affects the EOF of a coated capillary. A concentration of 100 mM or higher sodium chloride minimizes differences in EOF with different buffers and also gives sharper peaks for sample anions.
authors.affiliation: Ames Laboratory, US Department of Energy and Department of Chemistry, Iowa State University, 50011, USA. kniss@ameslab.gov
authors.forename: J S
authors.initials: JS
authors.lastname: Fritz
authors.forename: S A
authors.initials: SA
authors.lastname: Steiner
mesh_terms: D000838:Anions / Q000302:isolation & purification*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000838:Anions; D002021:Buffers; D011108:Polymers