High frequency loss of heterozygosity in the region of the DBH locus in bladder cancer.
OBJECTIVE
To determine the location of a putative tumor suppressor gene (TSG), and evaluate the frequency loss of heterozygosity (LOH) of the long arm of chromosome 9 (9q) in bladder cancer.


METHODS
We analyzed 25 patients with bladder cancer for LOH of 9q using 25 high-density microsatellite markers.


RESULTS
Twenty-three samples (92%) showed LOH at least at one locus on 9q. We identified that the commonly deleted region were at 9q12-q21, 9q22, and 9q34. The rate of LOH was 44.0%, 22.7%, 22.7%, 16.0%, 12.0% on DBH, D9S15, D9S1815, D9S1831, D9S176 locus, respectively, and was not significantly related with grades and stages of tumor.


CONCLUSIONS
These data suggest that alteration of a TSG at DBH of 9q may play an important role in the development of bladder cancer.
Yang
X
X
Department of Urology, First Affiliated Hospital, Shanxi Medical University, Taiyuan 030001, China.
Shen
P
P
Yao
K
K
Mi
Z
Z
Liu
S
S
Zhang
H
H
Wang
D
D
Liu
C
C
Liu
H
H
D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009367:Neoplasm Staging; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm