title: Efficient and accurate site-directed mutagenesis of large plasmids.
authors.affiliation: Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ontario, Canada.
authors.forename: Susan A
authors.initials: SA
authors.lastname: Nadin-Davis
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics; D015203:Reproducibility of Results; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D004262:DNA Restriction Enzymes