[Proinflammatory cytokine stimulated NF-kappa B activation and the effect of dexamethasone in the human airway epithelial cells].
OBJECTIVE
To observe the expression of NF-kappa B activation and the effect of dexamethasone on the NF-kappa B activity in the human airway epithelial cell line 16HBE after TNF-alpha stimulation.


METHODS
After 16HBE was treated with different concentration of TNF-alpha(10 U/ml, 100 U/ml, 1,000 U/ml) and dexamethasone (100 nmol/L), total RNA and cellular, nuclear protein were extracted at 1 hour, 2 hour, 4 hour, respectively. RT-PCR and electrophoresis mobility shift assay(EMSA) were used to detect the expression of IL-8 mRNA and NF-kappa B activation.


RESULTS
The activity of NF-kappa B activation became stronger at 1 hour in the TNF-alpha stimulated group than the control, peaked at 2 hours and then decreased at 4 hours. Supershift assay confirmed that both p50 and p65 were components of active NF-kappa B. At the same time, IL-8 mRNA expression was elevated at 4 hours. After dexamethasone treatment, the expression of NF-kappa B activation and IL-8 mRNA became lower.


CONCLUSIONS
It is suggested that activated NF-kappa B played a key role in the inflammatory process of respiratory diseases through regulating the expression of some important factors (cytokines). Glucocorticoid inhibited the activation of NF-kappa B and showed antiinflammatory effect.
Zhang
Y
Y
Department of Pulmonary Medicine, Guangzhou Institute of Respiratory Diseases, Guangzhou 510120, China.
Xu
J
J
Zhong
N
N
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D016209:Interleukin-8; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone