title: Identification of a gene required for cis-to-trans carotene isomerization in carotenogenesis of the cyanobacterium Synechocystis sp. PCC 6803.
abstract: A disruptive mutant of the sll0033 gene of the cyanobacterium Synechocystis sp. PCC 6803 produced primarily cis carotenes and small amounts of all-trans carotenes, but no xanthophylls, under dark conditions. Under light conditions, however, it produced normal carotenoids, that were the same as those produced by wild-type cells grown under both light and dark conditions. When the mutant cells cultured under dark conditions were irradiated, cis-isomers of carotenes were converted to all-trans lycopene. These findings demonstrate that this gene, designated crtH, is involved in the isomerization of cis-carotenes to all-trans forms in dark conditions, and that cis-carotenes were also converted to all-trans forms under light conditions by photoisomerization.
authors.affiliation: Biological Laboratory, Faculty of Education, Kumamoto University, Kumamoto, 860-8555 Japan. masamoto@gpo.kumamoto-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Masamoto
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: S
authors.initials: S
authors.lastname: Takaichi
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002338:Carotenoids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D003624:Darkness; D008027:Light; D000077276:Lycopene; D009154:Mutation; D013057:Spectrum Analysis; D013237:Stereoisomerism; D019745:cis-trans-Isomerases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002338:Carotenoids; D019745:cis-trans-Isomerases; D000077276:Lycopene