title: Characteristics and mechanism of formation of peroxide-induced heme to protein cross-linking in myoglobin.
abstract: At acidic pH values heme-protein cross-linked myoglobin (Mb-H) forms as a product of a peroxide-induced ferric-ferryl redox cycle. There is evidence that this molecule acts as a marker for heme-protein-induced oxidative stress in vivo and may exacerbate the severity of oxidative damage due to its enhanced prooxidant and pseudoperoxidatic activities. Therefore, an understanding of its properties and mechanism of formation may be important in understanding the association between heme-proteins and oxidative stress. Although the mechanism of formation of heme-protein cross-linked myoglobin is thought to involve a protein radical (possibly a tyrosine) and the ferryl heme, we show that this hypothesis needs revising. We provide evidence that in addition to a protein-based radical the protonated form of the oxoferryl heme, known to be highly reactive and radical-like in nature, is required to initiate cross-linking. This revised mechanism involves radical/radical termination rather than attack of a single radical onto the porphyrin ring. This proposal better explains the pH dependence of cross-linking and may, in part, explain the therapeutic effectiveness of increasing the pH on myoglobin-induced oxidative stress, e.g., therapy for rhabdomyolysis-associated renal dysfunction.
authors.affiliation: Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, United Kingdom. reedb@essex.ac.uk
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Reeder
authors.forename: Dimitri A
authors.initials: DA
authors.lastname: Svistunenko
authors.forename: Martyn A
authors.initials: MA
authors.lastname: Sharpe
authors.forename: Michael T
authors.initials: MT
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002074:Butanones / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000378:metabolism*; D006736:Horses; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008786:Metmyoglobin / Q000378:metabolism; D009211:Myoglobin / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding
chemical_list: D002074:Butanones; D003432:Cross-Linking Reagents; D009211:Myoglobin; C017595:oxymyoglobin; D008786:Metmyoglobin; D006418:Heme; D006861:Hydrogen Peroxide