Generic microcystin immunoassay based on monoclonal antibodies against Adda.
A monoclonal antibody (clone AD4G2) was generated against a common part of microcystins and nodularins, the unusual amino acid Adda [(2S,3S,8S,9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4E,6E-dienoic acid]. A direct competitive ELISA based on this antibody was developed and the cross-reactivity pattern was measured. Different toxins showed a very similar response. The assay provides therefore a sum parameter of microcystins, nodularins and peptide fragments containing Adda. The IC50 for microcystin-LR was 0.33 microg L(-1) which leads to a detection limit of 0.07 microg L(-1). This is well below the concentration of 1 microg L(-1) proposed by the World Health Organisation (WHO) as the limit for drinking water. Microcystin-LR spiked water samples in the concentration range between 0.1 and 1 microg L(-1) were measured and a mean recovery of 113+/-23% was found. The antibody is well suited for the determination of microcystins in drinking as well as surface water.
Zeck
A
A
Institute of Hydrochemistry, Technical University of Munich, Germany.
Weller
M G
MG
Bursill
D
D
Niessner
R
R
D000577:Amides / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002210:Caprylates / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006825:Hybridomas; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis*
C444580:Adda precursor; D000577:Amides; D000911:Antibodies, Monoclonal; D002210:Caprylates; D052998:Microcystins; D010456:Peptides, Cyclic; D014873:Water Pollutants; C078588:microcystin