Prevention of development of N,N'-dimethylhydrazine-induced colon tumors by a water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi) mycelia in male ICR mice.
The protective effects of a dietary water-soluble extract from cultured medium of Ganoderma lucidum (Rei-shi or Mannentake) mycelia (designated as MAK) against development of colon tumors were investigated in male ICR mice. The animals were given weekly injections of N,N'-dimethylhydrazine (DMH, 10 mg/kg body weight) for the initial 10 weeks to induce colon carcinogenesis, and then fed on diet with or without 5% MAK for 10 weeks. There were no significant differences in incidence and the total number of colon tumors between the groups. However, the MAK diet group demonstrated significantly reduced sizes of tumors in comparison with the MF diet group. Moreover, this was linked to a lowered PCNA positive index and shortening of the germinal region in the colon. beta-catenin positive tumor cell nuclei were also significantly decreased in the MAK group. The present results thus indicate that dietary MAK could act as a potent chemopreventive agent for colon carcinogenesis.
Lu
Huimei
H
Department of Environment and Mutation, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-8553, Japan.
Kyo
Eikai
E
Uesaka
Toshihiro
T
Katoh
Osamu
O
Watanabe
Hiromitsu
H
D019813:1,2-Dimethylhydrazine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001835:Body Weight; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003598:Cytoskeletal Proteins / Q000032:analysis; D004032:Diet; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D020940:Reishi / Q000737:chemistry* / Q000166:cytology; D012995:Solubility; D015534:Trans-Activators; D051176:beta Catenin
D016588:Anticarcinogenic Agents; C495267:CTNNB1 protein, mouse; D002457:Cell Extracts; D003598:Cytoskeletal Proteins; D004365:Drugs, Chinese Herbal; D015534:Trans-Activators; D051176:beta Catenin; D019813:1,2-Dimethylhydrazine