title: Myosin light chain kinase as a multifunctional regulatory protein of smooth muscle contraction.
abstract: Myosin light chain kinase (MLCK) is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. Although this mode of action is well-established, there are numerous reports of smooth muscle contraction that is not associated with MLC20 phosphorylation. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. This article overviews as to how such multifunctional properties of MLCK modify the actin-myosin interaction and presents our observations that the phosphorylation is not obligatory in induction of smooth muscle contraction.
authors.affiliation: Department of Pharmacology, Gunma University School of Medicine, Maebash, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: L H
authors.initials: LH
authors.lastname: Ye
authors.forename: H
authors.initials: H
authors.lastname: Kishi
authors.forename: T
authors.initials: T
authors.lastname: Okagaki
authors.forename: K
authors.initials: K
authors.lastname: Samizo
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Kohama
mesh_terms: D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002147:Calmodulin / Q000378:metabolism; D002384:Catalysis; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000201:enzymology / Q000502:physiology*; D009210:Myofibrils / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D000199:Actins; D002147:Calmodulin; D011994:Recombinant Proteins; D009219:Myosin-Light-Chain Kinase; D000251:Adenosine Triphosphatases