title: [Detection of TDH-producing Vibrio parahaemolyticus O3:K6 from naturally contaminated shellfish using an immunomagnetic separation method and chromogenic agar medium].
abstract: We attempted to isolate TDH-producing Vibrio parahaemolyticus O3:K6 from shellfish. Asari samples were incubated with TSB supplemented with 2% (w/v) NaCl for 6 h, and then the 6-h cultures were incubated with salt polymyxin broth for 18 h. After the two-step enrichment, a 1 ml portion of the culture was treated with magnetic beads coated with K6 antibody for immunoconcentration of V. parahaemolyticus O3:K6. The immunoconcentrated and untreated cultures were plated onto a chromogenic agar and TCBS agar media for isolation of V. parahaemolyticus. TDH-producing V. parahaemolyticus O3:K6 was isolated from 3 out of 66 lots (4.5%) of naturally contaminated Asari. Six of 4,265 colonies suspected as V. parahaemolyticus (0.14%) were TDH-producing V. parahaemolyticus O3:K6.
authors.affiliation: Shizuoka Institute of Environment and Hygiene.
authors.forename: Y
authors.initials: Y
authors.lastname: Hara-Kudo
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: T
authors.initials: T
authors.lastname: Nishina
authors.forename: A
authors.initials: A
authors.lastname: Saitoh
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Ichihara
authors.forename: H
authors.initials: H
authors.lastname: Konuma
authors.forename: J
authors.initials: J
authors.lastname: Hasegawa
authors.forename: S
authors.initials: S
authors.lastname: Kumagai
mesh_terms: D000362:Agar; D001427:Bacterial Toxins; D001431:Bacteriological Techniques / Q000379:methods; D002863:Chromogenic Compounds; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D018189:Immunomagnetic Separation; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D002863:Chromogenic Compounds; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin; D000362:Agar