[Ligand of platelet and T cell activation antigen 1 (PTA1) expressed on Colo205 cell surface].
OBJECTIVE
To confirm the existence of PTA1 ligand (PTA1L).


METHODS
PTA1/Ig fusion protein was prepared by gene recombination and expression in COS-7 cells by DEAE-dextran transfection. The fusion protein was used as a probe in the investigation of PTA1L by immunohistochemical staining. Existence of PTA1L was further identified by adhesion experiment.


RESULTS
Colo205 cells could be stained by PTA1/Ig fusion protein specifically but not by hIg, and the adhesion of activated Jurkat cells with Colo205 could be blocked by PTA1/Ig and PTA1 mAb.


CONCLUSIONS
PTA1 ligand is present on the surface of Colo205 cells. These results provide a solid basis for further investigation of the role of PTA1-PTA1L interaction in the pathogenesis of neoplasm.
Sun
K
K
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Jin
B
B
Feng
Q
Q
D000818:Animals; D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D019556:COS Cells; D003110:Colonic Neoplasms; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007150:Immunohistochemistry; D019169:Jurkat Cells; D008024:Ligands; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured; D000098862:T Lineage-Specific Activation Antigen 1
D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D007136:Immunoglobulins; D008024:Ligands; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C445782:platelet and T cell antigen 1 ligand