Multiple inductive signals are involved in the development of the ctenophore Mnemiopsis leidyi.
Ctenophores possess eight longitudinally arrayed rows of comb plate cilia. Previous intracellular cell lineage analysis has shown that these comb rows are derived from two embryonic lineages, both daughters of the four e(1) micromeres (e(11) and e(12)) and a single daughter of the four m(1) micromeres (the m(12) micromeres). Although isolated e(1) micromeres will spontaneously generate comb plates, cell deletion experiments have shown that no comb plates appear during embryogenesis following the removal of e(1) descendents. Thus, the m(1) lineage requires the inductive interaction of the e(1) lineage to contribute to comb plate formation. Here we show that, although m(12) cells are normally the only m(1) derivatives to contribute to comb plate formation, m(11) cells are capable of generating comb plates in the absence m(12) cells. The reason that m(11) cells do not normally make comb rows may be attributable either to their more remote location relative to critical signaling centers (e.g., e(1) descendants) or to inhibitory signals that may be provided by other nearby cells such as sister cells m(12). In addition, we show that the signals provided by the e(1) lineage are not sufficient for m(1)-derived comb plate formation. Signals provided by endomesodermal progeny of either the E or the M lineages (the 3E or 2M macromeres) are also required.
Henry
J Q
JQ
Department of Cell and Structural Biology, University of Illinois, 601 S. Goodwin Avenue, Urbana, Illinois 61801, USA. j-henry4@uiuc.edu
Martindale
M Q
MQ
D000818:Animals; D005075:Biological Evolution; D001755:Blastocyst / Q000378:metabolism; D019521:Body Patterning; D019070:Cell Lineage; D004707:Endoderm / Q000378:metabolism; D007448:Invertebrates / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D008648:Mesoderm / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D013997:Time Factors