[The role of exogenous hRAR beta gene on the growth inhibition and differentiation induction of HL-60R Cells].
OBJECTIVE
To investigate the role of hRAR beta gene in HL-60R cells and in their response to retinoids.


METHODS
By electroporation, the recombinant pcDNA3-hRAR beta plasmid was transfected into the hRNA beta gene not expressed human myleocytic leukemic cell line HL-60R. In the presence of RAR-selected agonist Ch55, the effect of RAR beta gene on HL-60R was studied by the efficiency of colony formation in soft agar, cell morphology NBT reduction assay and flow cytometry.


RESULTS
The HL60R-hRAR beta cells with stable hRAR beta gene expression were obtained. The growth and colony formation in soft agar of HL60R-hRAR beta cells were remarkably reduced as compared with that of their parent cells, and the cells were induced into granulocytic differentiation by Ch55 treatment at 10(-7).


CONCLUSIONS
The expression of exogenous hRAR beta gene in HL-60R cells could resume their sensitivity to retionoids in inhibiting cell proliferation and inducing granulocytic differentiation.
Chen
R
R
Department of Hematology, Affiliated Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Yin
X
X
Chen
F
F
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D047188:Chalcones; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D018922:HL-60 Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000502:physiology*; D014162:Transfection
D047188:Chalcones; D018168:Receptors, Retinoic Acid; C082925:retinoic acid receptor beta; D002599:Chalcone; C054189:3,5-di-tert-butylchalcone 4'-carboxylic acid