[Study on antitumor activity and mechanism of Ganoderma polysaccharides B].
OBJECTIVE
To study the antitumor activity and mechanism of Ganoderma polysaccharides (GL-B).


METHODS
Both in vivo and in vitro experiments were conducted in the study. Proliferation of tumor cells was detected by MTT method, tumor necrosis factor alpha (TNF alpha) was detected by biological assay, and interferon gamma (IFN gamma) by enzyme-linked immunosorbent assay (ELISA). The level of mRNA was detected with the method of reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
(1) GL-B 50, 100, 200 micrograms/ml inhibited the growth of implanted sarcoma 180 in vivo significantly and dose-dependently. (2) GL-B directly adding to the cultured medium neither induced HL-60 apoptosis nor restrained its proliferation in vitro. (3) The macrophages and T lymphocytes cultured medium treated with GL-B significantly induced HL-60 apoptosis and inhibited its proliferation. GL-B significantly increased TNF alpha and IFN gamma release and their mRNA expression in the cultured medium.


CONCLUSIONS
The anti-tumor activity of GL-B was derived from promoting mRNA expression of TNF alpha and IFN gamma, resulting in TNF alpha and IFN gamma release.
Zhang
Q
Q
Department of Pharmacology, School of Basical Medical Sciences, Beijing Medical University, Beijing (100083).
Lin
Z
Z
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012510:Sarcoma 180 / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma