title: Overexpression of a novel gene, Cms1, can rescue the growth arrest of a Saccharomyces cerevisiae mcm10 suppressor.
abstract: MCM10 protein is an essential replication factor involved in the initiation of DNA replication. A mcm10 mutant (mcm10-1) of budding yeast shows a growth arrest at 37 degrees C. In the present work, we have isolated a mcm10-1 suppressor strain, which grows at 37 degrees C. Interestingly, this mcm10-1 suppressor undergoes cell cycle arrest at 14 degrees C. A novel gene, YLR003c, is identified by high-copy complementation of this suppressor. We called it as Cms1 (Complementation of Mcm 10 Suppressor). Furthermore, the experiments of transformation show that cells of mcm10-1 suppressor with high-copy plasmid but not low-copy plasmid grow at 14 degrees C, indicating that overexpression of Cms1 can rescue the growth arrest of this mcm10 suppressor at non-permissive temperature. These results suggest that CMS1 protein may functionally interact with MCM10 protein and play a role in the regulation of DNA replication and cell cycle control.
authors.affiliation: Institute of Biochemistry and Cell Biology, Shanghai Research Center of Life Science, Chinese Academy of Sciences.
authors.forename: J W
authors.initials: JW
authors.lastname: Wang
authors.forename: J R
authors.initials: JR
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D002868:Chromosomal Proteins, Non-Histone; D003001:Cloning, Molecular; D005800:Genes, Fungal; D016153:Genes, Suppressor; D064110:Minichromosome Maintenance Proteins; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C461859:Cms1 protein, S cerevisiae; C076365:MCM10 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D064110:Minichromosome Maintenance Proteins