[Study on anti-HBV effects by antisense oligodeoxynucleotides in vitro].
OBJECTIVE
To study the inhibition effect of anti-HIV oligodeoxy-nucleotides on HBV X gene.


METHODS
Three antisense phosphorothioate oligodeoxy-nucleotides(AsON) complementary to the initiator of X, DR2, EN II respectively, were synthesized and tested for their anti-HBV activity on HepG2.2.15 cells with ELISA methods.


RESULTS
When the best effect concentration of AsON was 10 mumol/L, the inhibition rates on HBsAg of three AsON were 57%, 60% and 52% respectively, and on HBeAg were 56%, 45% and 56% respectively. There were two inhibition peaks at different times. There was no inhibition effect treated by random control (< 12%). Using the 3-(4,5-dimethythiazol-xyl)-2,5-diphnyl tetrazolium bromide method, there was no cytotoxicity at 40 mumol/L of AsON.


CONCLUSIONS
AsONs on three key region of X gene were effective drugs to inhibit the expression of HBV.
Liu
S
S
Department of Microbiology and Immunology, Shandong University, Ji'nan 250012, China.
Sun
W
W
Cao
Y
Y
D000998:Antiviral Agents / Q000494:pharmacology*; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D015534:Trans-Activators / Q000037:antagonists & inhibitors* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D020319:Oligodeoxyribonucleotides, Antisense; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein