[Construction of the shuttle vector pSTS1 and development of the method of its introduction into the cells of the cyanbacterium Plectonema boryanum CALU 465].
It has been shown that cells of trichoma cyanobacterium Plectonema boryanum G o m. strain CALU 465 do not possess natural resistance to antibiotics ampicyllin, chloramphenicol, streptomycin and canamycin. A shuttle vector pSTS1 has been constructed on the basis of cyanobacterial plasmid pSM1 and vector pBR 322. Conditions have been developed and conjugative transfer of pSTS1 to the cells of cyanobacterium P. boryanum has been performed. As the result of expression of beta-lactamase gene pSTS1 the resistance of cells-conjugantes to ampicillin increased by three orders.
Syrchin
S A
SA
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Samoĭlenko
V A
VA
Tsymbal
N V
NV
Mendzhul
M I
MI
D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000458:Cyanobacteria / Q000235:genetics*; D004352:Drug Resistance, Microbial; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D000667:Ampicillin; D001618:beta-Lactamases
Konstruirovanie chelnochnogo vektora pSTS1 i otrabotka sposoba ego vvedeniia v kletki tsianobakterii Plectonema boryanum GOM. shtamm CALU 465.