title: Neutrophil functions and serum IgG in growing foals.
abstract: The aim of this study was to investigate the phagocytic and killing capacities as well as expression of CD18 of neutrophils obtained from healthy foals from birth to age 8 months. Blood was taken from 6 Standardbred foals at 7 time-points between ages 2-56 days and thereafter once a month. For comparison, cells from 16 mature horses were evaluated. Neutrophil phagocytosis of yeast cells was assessed by flow cytometry after opsonisation with mature pooled serum, autologous serum or anti-yeast IgG. The killing capacity of the neutrophils, as indicated by the oxidative burst, was monitored by chemiluminescence. Serum IgG concentration was measured by radial immunodiffusion. In addition to clinical examination, the amount of serum amyloid A and the total leucocyte count were used as markers for infection. The phagocytic ability was impaired until age 3 weeks, when autologous serum was used as opsonin. Killing capacity was also low initially but, from 3 months onwards, chemiluminescence values were equal to or higher than in mature horses. Serum IgG decreased from 10 g/l at 2 days to 5 g/l at 2 months and then increased gradually to 10 g/l at the end of the study. These findings may in part explain the increased susceptibility to bacterial infections in young horses.
authors.affiliation: Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala.
authors.forename: S
authors.initials: S
authors.lastname: Demmers
authors.forename: A
authors.initials: A
authors.lastname: Johannisson
authors.forename: G
authors.initials: G
authors.lastname: Gröndahl
authors.forename: M
authors.initials: M
authors.lastname: Jensen-Waern
mesh_terms: D000375:Aging / Q000097:blood / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D018821:CD18 Antigens / Q000096:biosynthesis / Q000276:immunology; D004198:Disease Susceptibility / Q000209:etiology / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D006734:Horse Diseases / Q000276:immunology; D006736:Horses / Q000276:immunology*; D005779:Immunodiffusion / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D008163:Luminescent Measurements; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis / Q000276:immunology*; D016897:Respiratory Burst; D000685:Serum Amyloid A Protein / Q000032:analysis; D014886:Weaning
chemical_list: D018821:CD18 Antigens; D007074:Immunoglobulin G; D000685:Serum Amyloid A Protein