Combining site-specific chemical modification with site-directed mutagenesis. Versatile strategy to move beyond structural limitations of 20 natural amino acids side chains in protein engineering.
DeSantis
Grace
G
Diversa, San Diego, CA, USA.
Jones
J Bryan
JB
D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D011506:Proteins / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D007202:Indicators and Reagents; D011506:Proteins; D004247:DNA