[Study on the antitumor effect of angiostatin on LA795 adenocarcinoma cells].
OBJECTIVE
To study the antitumor effect of angiostatin on LA795 adenocarcinoma cells inoculated on T739 mice.


METHODS
Affinity chromatography purified plasminogen was digested by pancreatic elastase and angiostatin was obtained by purifying the digestion with affinity chromatography and dialysis. T739 mice were inoculated with LA795 cells. Angiostatin was given to part of inoculated and uninoculated mice by intraperitoneal injection fourteen days after the inoculation. The treatment lasted 20 days and the size of tumor, survival period, behavior of the mice and pathology of lungs, livers and kidneys were observed.


RESULTS
The size of tumor decreased from (2.35 +/- 0.26) cm to (0.97 +/- 0.34) cm after angiostatin treatment. The number of metastases in lung was significantly fewer in angiostatin treated mice than in untreated ones, which lived much shorter. No obvious side effects were observed.


CONCLUSIONS
Angiostatin markedly inhibits the growth and metastasis of LA795 cells on T739 mice and no obvious side effects of angiostatin were found during the treatment.
Zheng
F
F
Department of Respiratory Medicine, Nan Fang Hospital, Guangzhou 510515, China.
Tong
W
W
Wen
J
J
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D043168:Angiostatins; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D010446:Peptide Fragments / Q000627:therapeutic use*; D010958:Plasminogen / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D010446:Peptide Fragments; D043168:Angiostatins; D010958:Plasminogen