Assessment of clarithromycin-resistant Helicobacter pylori among patients in Shanghai and Guangzhou, China, by primer-mismatch PCR.
Of 96 Helicobacter pylori isolates from patients in Shanghai and Guangzhou, China, 5 had the A2143G 23S rRNA mutation as determined by primer-mismatch PCR and were resistant to clarithromycin by the E-test. The remaining isolates were primer-mismatch PCR negative and susceptible to clarithromycin. The conclusion is that the prevalence of clarithromycin-resistant H. pylori isolates among these Chinese patients is 5%.
Pan
Zhi-Jun
ZJ
Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands.
Su
Wan-Wen
WW
Tytgat
Guido N J
GN
Dankert
Jacob
J
van der Ende
Arie
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020137:Base Pair Mismatch; D002681:China / Q000453:epidemiology; D017291:Clarithromycin / Q000494:pharmacology*; D017931:DNA Primers; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D000900:Anti-Bacterial Agents; D017931:DNA Primers; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin