[Effect of gandou decoction on copper metabolism of skin fibroblast of hepatolenticular degeneration model].
OBJECTIVE
To explore the cytological mechanism of Gandou Decoction (GDD) in treating hepatolenticular degeneration (HLD).


METHODS
Twenty-three in vitro models of cultured skin fibroblast of HLD were established to observe the intracellular change of microelements (Cu2+, Zn2+, etc.) content before and after adding rabbit's serum containing GDD.


RESULTS
After being treated with GDD contained serum for 24 hours, the intracellular content of Cu2+ decreased from (80.94 +/- 34.76) ng/mg to (46.90 +/- 22.14) ng/mg, P < 0.01, while that of Zn2+ increased from (140.43 +/- 33.81) ng/mg to (151.43 +/- 37.83) ng/mg, P < 0.01.


CONCLUSIONS
GDD is quite effective in removing the intracellular copper and increasing the intracellular zinc.
Tang
Q
Q
Department of Neurology, Affiliated Hospital of Bengbu Medical College, Anhui (233004).
Yang
R
R
Han
Y
Y
D000293:Adolescent; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D003300:Copper / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D006527:Hepatolenticular Degeneration / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012867:Skin / Q000378:metabolism / Q000473:pathology*
D004365:Drugs, Chinese Herbal; D003300:Copper