[Role of costimulating molecule CD86 in maternal-fetal immune regulation].
OBJECTIVE
To evaluate the role of costimulating molecule CD86 in maternal-fetal immune regulation.


METHODS
In abortion-prone (CBA x DBA/2) matings and non-abortion-prone (CBA x BALB/c) matings, the pregnant CBA females were injected intraperitoneally by purified rat anti-murine CD86 monoclonal antibody (mAb) with dosage of 100 micrograms at day 4, 6, 8 and 10 of gestation respectively, or injected only once at day 4 of gestation, or injected with purified rat IgG as control. The resorbing and non-resorbing embryos were enumerated at day 14 of gestation.


RESULTS
The embryo resorbing rate in CBA x DBA/2 matings was 27.78%, and that in CBA x BALB/c matings was 8.42%. After injection of anti-CD86 mAb at day 4, 6, 8, 10 of gestation, the embryo resorbing rate reduced to 9.68% (P < 0.005) in CBA x DBA/2 matings, while that was 13.54% in CBA x BALB/c matings (P > 0.05). After injection of anti-CD86 mAb only once at day 4 of gestation, the embryo resorbing rate reduced apparently to 7.14% (P < 0.001) in CBA x DBA/2 matings, while that was 11.39% (P > 0.05) in CBA x BALB/c matings.


CONCLUSIONS
Blocking the costimulating signal by anti-CD86 mAb at maternal-fetal interface, especially at the prime stage of maternal-fetal recognition (periods of implantation), may result in maternal-fetal tolerance, and hopefully the embryo resorption turns to normal pregnancy.
Zhu
X
X
Laboratory of Reproductive Immunology, Institute of Obstetrics and Gynecology, Shanghai Medical University, Shanghai 200011, China.
Li
D
D
Sun
X
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D051940:B7-2 Antigen; D005260:Female; D007112:Immunity, Maternally-Acquired / Q000502:physiology*; D008297:Male; D008431:Maternal-Fetal Exchange; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins