Analysis of the 5S RNA pool in Arabidopsis thaliana: RNAs are heterogeneous and only two of the genomic 5S loci produce mature 5S RNA.
One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5'- and 3'-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.
Cloix
Catherine
C
U.M.R. 6547 BIOMOVE, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France.
Tutois
Sylvie
S
Yukawa
Yasushi
Y
Mathieu
Olivier
O
Cuvillier
Claudine
C
Espagnol
Marie-Claude
MC
Picard
Georges
G
Tourmente
Sylvette
S
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic
D017931:DNA Primers; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012332:RNA, Heterogeneous Nuclear; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S