Dual-substrate utilization by Bordetella pertussis.
To improve the cultivation of Bordetella pertussis and take advantage of the newest techniques in monitoring and control, a quantitative description of substrate utilisation is necessary. Growth of the organism is limited by two main substrates. However neither interactive nor non-interactive modelling seem appropriate. A model that combines essential and enhanced kinetics was developed based on experimental observation. Instead of fitting all model parameters at once, a step-wise experimentation procedure was used. Finally two cultivations showed the accuracy of the model.
Neeleman
R
R
National Institute of Public Health and the Environment, Laboratory for Product and Process Development, Bilthoven, The Netherlands. Ronald.Neeleman@rivm.nl
Joerink
M
M
Beuvery
C
C
van Boxtel
T
T
D018533:Biomass; D001886:Bordetella pertussis / Q000254:growth & development* / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008954:Models, Biological
D019344:Lactic Acid; D018698:Glutamic Acid