title: Analysis of mural cell recruitment to tumor vessels.
abstract: BACKGROUND
  Tumor blood vessels are both structurally and functionally abnormal compared with normal vessels. A limited support of mural cells may contribute to these abnormalities. Here, we characterized mural cell recruitment in 2 mouse tumor models and addressed the question of why tumor vessels fail to recruit a proper coat of mural cells.
  
  
  RESULTS
  We studied mural cell recruitment to the vasculature of 2 transplantable mouse tumor models, T241 fibrosarcoma and KRIB osteosarcoma. We found that both tumors formed a vessel network with heterogeneous and highly abnormal organization of mural cells. Transplantation of tumors to mice expressing lacZ in mural cells demonstrated that these cells were host-derived. Although tumor vessel endothelium expressed PDGF-B, an embryonic mitogen for mural cells, only very few PDGFRbeta-positive cells were found to be associated with the developing tumor vasculature, suggesting a limited pool of recruitable mural cells. We tested whether exogenous mural cells could be recruited to tumor vessels by injecting mixtures of T241 tumor cells and embryonic mesenchymal cells isolated from mice expressing lacZ in mural cells. In the tumors that arose, lacZ-positive cells were efficiently recruited to the tumor vessels.
  
  
  CONCLUSIONS
  T241 and KRIB tumors show a similar highly abnormal organization of vessel-associated mural cells. T241 tumor vessels seem highly capable of recruiting exogenously added mural cells. The sparse mural cell coat of tumor vessels may result from a limited pool of mural cells available for recruitment.
authors.affiliation: Department of Medical Biochemistry, Göteborg University, Göteborg, Sweden.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Abramsson
authors.forename: Orjan
authors.initials: O
authors.lastname: Berlin
authors.forename: Hayk
authors.initials: H
authors.lastname: Papayan
authors.forename: Denise
authors.initials: D
authors.lastname: Paulin
authors.forename: Moshe
authors.initials: M
authors.lastname: Shani
authors.forename: Christer
authors.initials: C
authors.lastname: Betsholtz
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002460:Cell Line; D004730:Endothelium, Vascular / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009130:Muscle, Smooth / Q000737:chemistry; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D009369:Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D009374:Neoplasms, Experimental / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D020286:Pericytes / Q000473:pathology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D020797:Receptor, Platelet-Derived Growth Factor beta