Cellular screening assays using fluorescence microscopy.
The recent development of automated fluorescence imaging systems has enabled fluorescence microscopy to be used for the purposes of compound screening. This information-rich technique has found various applications, including screening for the effect of kinase inhibitors on the cytoskeleton, agonist-stimulated receptor internalization, and protein phosphorylation and acetylation. The discovery of novel fluorescent proteins and new fluorescent dyes will find many applications in multichannel fluorescence imaging assays.
Blake
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Sugen Inc., South San Francisco, CA 94080-4811, USA. robert-blake@sugen.com
D000818:Animals; D002477:Cells / Q000737:chemistry* / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes