Radiation-induced apoptosis of oligodendrocytes in the adult rat brain.
Although radiation injuries to the brain are well documented, immediate early histological changes in the brain remain to be defined. The present study characterizes glial injury provoked in adult rat white matter within 24 h after a single irradiation of the whole brain (10 or 20 Gray). Irradiated brains were histologically and histochemically analyzed. TUNEL-positive cells exhibiting apoptotic morphology were counted in five representative regions of the white matter. Glial cell death was further evaluated by glial cell density 24 h after irradiation, which induced both dose (p < 0.0001)- and time- (p < 0.0001) dependent apoptosis in these cells. The overall apoptotic rate in the white matter peaked within 8 h after irradiation. Total glial cell density decreased significantly in the white matter 24 h after irradiation. TUNEL-positive cells were immunohistochemically negative for GFAP, a marker for astrocytes, but positive for CNP, a marker for oligodendrocytes. The apoptotic rate was highest in the external capsule (p < 0.0001), followed by the fimbria and genu of the corpus callosum (p < 0.0001). The rates were lowest in the internal capsule and cerebellum. These data indicated that brain irradiation induces rapid apoptotic depletion of the oligodendroglial population, which may participate in the development of radiation-induced pathological conditions.
Kurita
H
H
Laboratory for Neuroscience and Neurooncology, Department of Neurosurgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Kawahara
N
N
Asai
A
A
Ueki
K
K
Shin
M
M
Kirino
T
T
D000367:Age Factors; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001921:Brain / Q000473:pathology / Q000528:radiation effects; D002452:Cell Count; D019583:Dose Fractionation, Radiation; D004307:Dose-Response Relationship, Radiation; D020287:In Situ Nick-End Labeling; D008297:Male; D009836:Oligodendroglia / Q000166:cytology* / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar