Identification of a novel protease inhibitor gene that is highly expressed in the prostate.
A novel gene was identified 52 kb upstream of the gene encoding the protease inhibitor elafin (PI3) on human chromosome 20q12-13.1. The transcript of the new gene, denoted huWAP2, was characterized by rapid amplification of cDNA ends and DNA sequencing. The size is 774 bp and it gives rise to a polypeptide of 111 amino acid residues that is homologous to elafin and similar WAP-type protease inhibitors. By RT-PCR it was shown that the gene is highly expressed in prostate, skin, lung, and esophagus.
Lundwall
Ake
A
Department of Laboratory Medicine, Lund University, University Hospital MAS, S-205 02 Malmö, Sweden. Ake.Lundwall@klkemi.mas.lu.se
Clauss
Adam
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002890:Chromosomes, Human, Pair 20; D018076:DNA, Complementary / Q000378:metabolism; D053492:Elafin; D005091:Exons; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011467:Prostate / Q000378:metabolism*; D011480:Protease Inhibitors / Q000737:chemistry*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; D053492:Elafin; C506806:PI3 protein, human; D011480:Protease Inhibitors; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; C447606:WFDC12 protein, human