[The mechanism of docetaxel-induced apoptosis in human lung cancer cells].
OBJECTIVE
To study the mechanism of docetaxel-induced apoptosis.


METHODS
Morphological study, DNA gel electrophoresis, flow cytometry and fluorescin labeled Annexin V to detect apoptosis, RT-PCR to detect the gene related with apoptosis.


RESULTS
Human lung cancer A549 cells treated with docetaxel induced cell cycle arrest at G2M phase, leading to apoptosis. The morphology of A549 showed nuclear chromatine condensation and fragmentation. Typical ladder pattern of DNA fragmentation was observed. Sub-G1 peak was found by flow cytometry. Transcription of Fas gene was enhanced, while no change in c-myc and bcl-2 genes. Annexin labeling results revealed the co-existence of cell apoptosis and necrosis in docetaxel-treated A549 cells.


CONCLUSIONS
Docetaxel induces apoptosis and necrosis of human lung cancer. The induction of apoptosis may be related to expression of Fas.
Li
Y
Y
PLA General Hospital, Beijing 100853, China.
Shi
T
T
Zhao
W
W
D017304:Annexin A5 / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D004273:DNA, Neoplasm / Q000737:chemistry / Q000187:drug effects*; D000077143:Docetaxel; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D043823:Taxoids; D014407:Tumor Cells, Cultured
D017304:Annexin A5; D000972:Antineoplastic Agents, Phytogenic; D051017:Apoptosis Regulatory Proteins; D004273:DNA, Neoplasm; C421307:FAIM protein, human; D011506:Proteins; D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel