[Effects of endothelin-1 on the collagen synthesis of cultured human dental pulp cells].
OBJECTIVE
To study the effects of endothelin-1(ET-1) on the collagen synthesis of cultured human dental pulp cells.


METHODS
Cell cultured, enzyme linked immmun sorbent assay (ELISA), and ABC immunhistochemical staining were used in the study.


RESULTS
ET-1 increased the synthesis of soluble type I collagen in different periods besides the 24 h group, and had no effects on the type III collagen; immunohistochemical staining revealed that ET-1 also increased the synthesis of unsoluble type I collagen.


CONCLUSIONS
ET-1 can stimulate type I collagenic synthesis of human dental pulp cells and might have an effect on pulp self-restoration.
Yan
Y
Y
School of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Liu
Z
Z
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D024042:Collagen Type I / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019332:Endothelin-1 / Q000494:pharmacology*; D006801:Humans; D013997:Time Factors
D024042:Collagen Type I; D019332:Endothelin-1; D003094:Collagen; D001205:Ascorbic Acid