Identification of a novel HLA-B*2722 allele from a Filipino cell.
We present the novel HLA-allele B*2722 amplified and sequenced from a Filipino individual. This DNA was included several times in the International Cell Exchange, UCLA, and typed at the time as B*27 or B*2706 by the majority of the participating laboratories. The second HLA-B allele of this person is B*3802. B*2704/06 or B*2704/06/10 were suggested by approximately one-third of the laboratories. As we were investigating the intron 3 sequences of B*27 alleles, we also sequenced exon 4 of this Filipino DNA and found a single nucleotide exchange in exon 4 (pos. 704) which was not in concordance with the previous B*2706 typing. Our sequencing results showed that the coding sequence of B*2722 is identical to B*2706 in exon 1, 2 and 3. In exon 4 the B*2722 sequence is identical to B*2704. HLA-B*2704 differs from B*2706 in one base at position 704, a G in B*2704 and a C in B*2706, respectively.
Witter
K
K
Labor of Immunogenetics, University Munich, Munich, Germany. kwitter@med.uni.muenchen.de
Lau
M
M
Zahn
R
R
Scholz
S
S
Albert
E D
ED
D000483:Alleles; D001483:Base Sequence; D005805:Genes, MHC Class I; D015796:HLA-B27 Antigen / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010679:Philippines; D016133:Polymerase Chain Reaction
D015796:HLA-B27 Antigen