title: Fermentation characterization and flux analysis of recombinant strains of Clostridium acetobutylicum with an inactivated solR gene.
abstract: The effect of solR inactivation on the metabolism of Clostridium acetobutylicum was examined using fermentation characterization and metabolic flux analysis. The solR-inactivated strain (SolRH) of this study had a higher rate of glucose utilization and produced higher solvent concentrations (by 25%, 14%, and 81%, respectively, for butanol, acetone, and ethanol) compared to the wild type. Strain SolRH(pTAAD), carrying a plasmid-encoded copy of the bifunctional alcohol/aldehyde dehydrogenase gene (aad) used in butanol production, produced even higher concentrations of solvents (by 21%, 45%, and 62%, respectively, for butanol, acetone, and ethanol) than strain SolRH. Clarithromycin used for strain SolRH maintenance during SolRH(pTAAD) fermentations did not alter product formation; however, tetracycline used for pTAAD maintenance resulted in 90% lower solvent production.
authors.affiliation: Department of Chemical Engineering, Northwestern University, Evanston, IL 60208, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Harris
authors.forename: L
authors.initials: L
authors.lastname: Blank
authors.forename: R P
authors.initials: RP
authors.lastname: Desai
authors.forename: N E
authors.initials: NE
authors.lastname: Welker
authors.forename: E T
authors.initials: ET
authors.lastname: Papoutsakis
mesh_terms: D000096:Acetone / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics; D000444:Aldehyde Dehydrogenase / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism; D017291:Clarithromycin / Q000494:pharmacology; D003013:Clostridium / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial; D005818:Genetic Engineering; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D008954:Models, Biological; D010957:Plasmids / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012997:Solvents / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D000440:Butanols; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D012997:Solvents; D000096:Acetone; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase; D013752:Tetracycline; D017291:Clarithromycin; D005947:Glucose