title: New gene transfer methods.
abstract: The intentional introduction of recombinant DNA molecules into a living organism can be achieved in many ways. Viruses have been making a living by practicing gene transfer for millennia. Recently, man has gotten into the act. The paradigm employed is fairly straightforward. First, a way must be found to move genetic information across biological membrane barriers. Then, presumably, DNA repair mechanisms do the rest. The array of methods available to move DNA into the nucleus provides the flexibility necessary to transfer genes into cells as physically diverse as sperm and eggs. Some of the more promising alternative strategies such as sperm-mediated gene transfer, restriction enzyme-mediated integration, metaphase II transgenesis, and a new twist on retrovirus-mediated gene transfer will be discussed, among other methods.
authors.affiliation: Gene Evaluation and Mapping Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20750, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Wall
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002352:Carrier Proteins / Q000502:physiology; D004247:DNA / Q000235:genetics*; D018274:Electroporation; D018014:Gene Transfer Techniques / Q000662:veterinary*; D005822:Genetic Vectors; D008297:Male; D009696:Nucleic Acids / Q000502:physiology; D012190:Retroviridae / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D019076:Transgenes
chemical_list: D002352:Carrier Proteins; D009696:Nucleic Acids; D004247:DNA