title: BIA-MS-MS: biomolecular interaction analysis for functional proteomics.
abstract: One of the experimental processes of functional proteomics is the analysis of protein interaction. Here, we review a new analytical platform, BIA-MS, for protein interaction analysis. BIA-MS is an integration of a surface plasmon resonance biosensor for real-time interaction analysis and mass spectrometry for the subsequent identification of interacting molecules.
authors.affiliation: National Institute of Advanced Science and Technology, Biological Information Research Center (JBIRC), Tokyo, Japan. natsume@jbirc.aist.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Natsume
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Isobe
mesh_terms: D015374:Biosensing Techniques; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins