[Insulin-like growth factor 1 regulates collagen synthesis of periodontal ligament cells by a mechanism involving inhibition of poly(ADP-ribose) synthesis].
OBJECTIVE
To investigate the effect of IGF1 on collagen synthesis of PDL cells and the intracellular mechanism of IGF1 action on collagen synthesis.


METHODS
Cultured human periodontal ligament cells were used, collagen synthesis, lactate production, PADPR synthesis and PADPR synthetase activity under the action of IGF1 at various concentration for 16 hours were analysed.


RESULTS
(1) IGF1 stimulates collagen synthesis of cultured PDL cell. (2) IGF1 increases lactate production of PDL cells, which can be negated by 2-DG. (3) IGF1 decreases PADPR synthesis through inhibiting PADPR synthetase activity of PDL cells. (4) exogenous NAD+ attenuates stimulated collagen synthesis by IGF1 on PDL cells.


CONCLUSIONS
IGF1 stimulates collagen synthesis of PDL cells through inhibiting PADPR synthesis by acting at increasing lactate production which lowers intracellular NAD+ level and at suppressing PADPR synthetase activity. It strongly suggests that PADPR is an intracellular mediator of IGF1 action on collagen synthesis.
Zhang
J
J
College of Stomatology, West China University of Medical Science, Chengdu 610041.
Hussian
Z M
ZM
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D019344:Lactic Acid / Q000378:metabolism; D009243:NAD / Q000494:pharmacology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*
D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; D019344:Lactic Acid; D007334:Insulin-Like Growth Factor I; D003094:Collagen