Biomaterial patches sutured onto the rat stomach induce a set of genes encoding pancreatic enzymes.
Asymmetric patches of polyhydroxybutyric acid with one smooth and one rough surface were produced by a dipping procedure. These patches were implanted into the rat gastrointestine and tissue samples were generated at distinct time intervals after surgery. The host's response towards the foreign material was analyzed by Differential Display and RNA profiles were compared to each other. One to two weeks after surgery a group of mRNAs encoding pancreatic enzymes was transiently present after biomaterial implantation.
Löbler
Marian
M
Klinik und Poliklinik für Chirurgie der Universität Rostock, Forschugslabor, Germany. loebler@ch11.ukl.uni-freiburg.de
Sass
Marko
M
Kunze
Carmen
C
Schmitz
Klaus-Peter
KP
Hopt
Ulrich T
UT
D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials; D017931:DNA Primers; D005753:Gastric Mucosa / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008855:Microscopy, Electron, Scanning; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001672:Biocompatible Materials; D017931:DNA Primers