Identification of a gene essential for sheathed structure formation in Sphaerotilus natans, a filamentous sheathed bacterium.
Sphaerotilus natans, a filamentous bacterium that causes bulking in activated sludge processes, can assume two distinct morphologies, depending on the substrate concentration for growth; in substrate-rich media it grows as single rod-shaped cells, whereas in substrate-limited media it grows as filaments. To identify genes responsible for sheath formation, we carried out transposon Tn5 mutagenesis. Of the approximately 20,000 mutants obtained, 7 did not form sheathed structures. Sequencing of the Tn5-flanking regions showed that five of the seven Tn5 insertions converged at the same open reading frame, designated sthA. The deduced amino acids encoded by sthA were found to be homologous to glycosyltransferase, which is known to be involved in linking sugars to lipid carriers during bacterial exopolysaccharide biosynthesis. Disruption of the gene of the wild-type strain by inserting a kanamycin resistance gene cassette also resulted in sheathless growth under either type of nutrient condition. These findings indicate that sthA is a crucial component responsible for sheath formation.
Suzuki
Toshihiko
T
Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Central 6, 1-1-1 Higashi, Tsukuba 305-8566, Japan.
Kanagawa
Takahiro
T
Kamagata
Yoichi
Y
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D020562:Betaproteobacteria / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D017353:Gene Deletion; D020043:Genes, Essential / Q000235:genetics* / Q000502:physiology; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D016695:Glycosyltransferases