The role of perivascular and meningeal macrophages in experimental allergic encephalomyelitis.
The perivascular (PVM) and meningeal (MM) macrophages constitute a major population of resident macrophages in the central nervous system (CNS). To investigate a possible role of PVM and MM during CNS inflammation, we have analysed PVM and MM during experimental allergic encephalomyelitis (EAE), an experimental model for MS, in the rat. Our results demonstrate a remarkable increase in the expression of the ED2 antigen on PVM and MM (already at day 9 post-EAE induction), which precedes the onset of clinical symptoms and infiltration of leukocytes into the CNS (at day 13). Therefore, the onset of EAE is accompanied by alterations of PVM and MM, and the ED2 antigen provides an early marker of pathology during CNS inflammation. Moreover, selective depletion of the ED2-positive macrophages in the CNS using clodronate liposomes resulted in a suppression of the clinical symptoms. These observations indicate that PVM and MM play a role during the early stages of EAE development.
Polfliet
Machteld M J
MM
Department of Molecular Cell Biology, Faculty of Medicine, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands. M.Polfliet.cell@med.vu.nl
van de Veerdonk
F
F
Döpp
Ed A
EA
van Kesteren-Hendrikx
Esther M L
EM
van Rooijen
Nico
N
Dijkstra
Christine D
CD
van den Berg
Timo K
TK
D000208:Acute Disease; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D004002:Clodronic Acid / Q000494:pharmacology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005434:Flow Cytometry; D007276:Injections, Intraventricular; D008081:Liposomes; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008578:Meninges / Q000098:blood supply / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
D000963:Antimetabolites; D008081:Liposomes; D004002:Clodronic Acid