Analysis of a growth-promoting factor for Babesia caballi cultivation.
Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.
Ikadai
H
H
Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Martin
M D
MD
Nagasawa
H
H
Fujisaki
K
K
Suzuki
N
N
Mikami
T
T
Kudo
N
N
Oyamada
T
T
Igarashi
I
I
D000818:Animals; D001403:Babesia / Q000254:growth & development*; D016895:Culture Media, Serum-Free; D006133:Growth Substances
D016895:Culture Media, Serum-Free; D006133:Growth Substances