Comparison of different methods for the isolation of Burkholderia cepacia DNA from pure cultures and waste water.
DNA from Burkholderia cepacia was prepared from suspensions of pure cultures and artificially contaminated waste water. The efficacy of four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol extraction, microwave treatment, heat treatment) and six commercially available kits (Puregene, High Pure PCR Template Preparation Kit, InstaGene, QIAamp Tissue Kit, DNAzol, Elu-Quik) was compared in terms of sensitivity in a subsequent PCR. The results showed that a simple and inexpensive procedure using a lysis buffer containing proteinase K was superior to all other methods tested.
Merk
S
S
Institut für Mikrobiologie, Sanitätsakademie der Bundeswehr, Neuherbergstr. 11, 80937 München, Germany.
Neubauer
H
H
Meyer
H
H
Greiser-Wilke
I
I
D016956:Burkholderia cepacia / Q000235:genetics* / Q000302:isolation & purification; D018929:Cell Culture Techniques; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000032:analysis*
D004269:DNA, Bacterial; D014873:Water Pollutants