Combined use of LC/MS and a biological test for rapid identification of marine mycotoxins produced by Trichoderma koningii.
Trichoderma koningii Oudemans, a strain isolated from a shellfish farming area, was selected for its high frequency in samples and its ability to produce metabolites when cultured in natural seawater. Combined use of LC/MS and a biological test on blowfly larvae allowed the characterization of four compounds after purification in only two steps (VLC and HPLC). ESI/MS, a powerful tool for rapid identification and sequence determination of peptides, confirmed that these compounds were peptide, alpha-aminoisobutyric acid and amino alcohol (peptaibols), the usual metabolites of Trichoderma.
Landreau
Anne
A
ISOMer, SMAB-URM11 (Toxinologie marine), Faculté de Pharmacie, Université de Nantes, BP 53508, 44035 Nantes Cedex 01, France. Anne.Landreau@sante.univ-nantes.fr
Pouchus
Yves François
YF
Sallenave-Namont
Claire
C
Biard
Jean François
JF
Boumard
Marie Claude
MC
Robiou du Pont
Thibaut
T
Mondeguer
Florence
F
Goulard
Christophe
C
Verbist
Jean François
JF
D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis*; D014242:Trichoderma / Q000737:chemistry* / Q000472:pathogenicity
D009183:Mycotoxins; D010455:Peptides