Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
We describe, for the first time, a detailed electroporation procedure for Lactobacillus delbrueckii. Three L. delbrueckii strains were successfully transformed. Under optimal conditions, the transformation efficiency was 10(4) transformants per microg of DNA. Using this procedure, we identified several plasmids able to replicate in L. delbrueckii and integrated an integrative vector based on phage integrative elements into the L. delbrueckii subsp. bulgaricus chromosome. These vectors provide a good basis for developing molecular tools for L. delbrueckii and open the field of genetic studies in L. delbrueckii.
Serror
Pascale
P
Génétique Microbienne, INRA, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. serror@diamant.jouy.inra.fr
Sasaki
Takashi
T
Ehrlich
S Dusko
SD
Maguin
Emmanuelle
E
D001435:Bacteriophages / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D005822:Genetic Vectors; D007778:Lactobacillus / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics*; D012093:Replicon / Q000235:genetics; D014169:Transformation, Bacterial; D016662:Virus Integration