[Effects of mannose derivatives on development of selectin-dependent peritoneal inflammation in rats and mice].
The inhibitory effect of mannose-containing oligosaccharides on model carbohydrate ligand interaction with E-, P- and L-selectins in vitro, as well as on the ability of these compounds to block the leukocyte extravasation in rat and mouse peritonitis in vivo was studied. The monomeric and polymeric compounds, 4-nitrophenylthiomannoside, phenylmannoside, conjugated with polyacrylic acid, and alpha-mannose, conjugated with polyacrylamide, inhibited the binding of the model ligand to P- and L-selectins (but not to E-selectin). Intravenous injection of these compounds was found to cause a dose-dependent reduction of neutrophil accumulation in rat peritoneum. The polysaccharide mannan was inactive in both types of experiments. The conjugate of phenylmannoside with polyacrylic acid was the most effective blocker as in vitro experiments, as well as in vivo. The inhibitory effect of subcutaneous injection of 4-nitrophenylthiomannoside on mouse peritonitis was demonstrated.
Ushakova
N A
NA
Orehovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya 10, Moscow 119992.
Probrazhenskaia
M E
ME
Nifant'ev
N E
NE
Voznyĭ
Ia V
IaV
Pochechueva
T V
TV
Bovin
N V
NV
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D019040:E-Selectin / Q000737:chemistry / Q000378:metabolism; D019041:L-Selectin / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D009504:Neutrophils / Q000473:pathology; D019007:P-Selectin / Q000737:chemistry / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology; D010538:Peritonitis / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D019087:Selectins / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D019040:E-Selectin; D019041:L-Selectin; D008024:Ligands; D008358:Mannose; D019007:P-Selectin; D011994:Recombinant Proteins; D019087:Selectins; D000099166:Polyacrylamides; C006903:carbopol 940
Vliianie proizvodnykh mannozy na razvitie selektinzavisimogo peritoneal'nogo vospaleniia u krys i mysheĭ.