Factor H-related protein-5: a novel component of human glomerular immune deposits.
Factor H-related protein-5 (FHR-5) is a newly described human plasma protein with structural similarities to human factor H and the four other recently described factor H-related proteins. Unlike the other factor H-related proteins, FHR-5 was initially identified at the protein, rather than complementary DNA, level in studies of glomerular immune deposit composition. To determine the incidence and pattern of detection of FHR-5 in human renal biopsy specimens, a prospective study of 100 consecutive biopsies was performed using anti-FHR-5 monoclonal antibody and indirect immunofluorescence. The pattern of FHR-5 deposition is very similar to that of other complement components, C3 and SC5b-9. It is detected in all complement-containing glomerular immune deposits and has a pattern of extraglomerular deposition similar to that of other complement components. This study shows FHR-5 to be strongly associated with glomerular complement deposition and suggests a role in complement activation or regulation. It may be an important mediator of glomerular disease.
Murphy
Brendan
B
Department of Nephrology, St Vincent's Hospital, Melbourne, Victoria, Australia. murphybf@svhm.org.au
Georgiou
Toula
T
Machet
David
D
Hill
Prudence
P
McRae
Jennifer
J
D001798:Blood Proteins / Q000276:immunology / Q000378:metabolism*; D003176:Complement C3 / Q000276:immunology / Q000378:metabolism; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins / Q000276:immunology / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008856:Microscopy, Fluorescence
D001798:Blood Proteins; C432223:CFHR5 protein, human; D003176:Complement C3; D015938:Complement Membrane Attack Complex; D006023:Glycoproteins; C047610:SC5b-9 protein complex; D003165:Complement System Proteins