Direct and electrically wired bioelectrocatalysis by hydrogenase from Thiocapsa roseopersicina.
Hydrogen enzyme electrodes based on direct and mediated bioelectrocatalysis were developed. Direct bioelectrocatalysis of hydrogen oxidation/evolution was observed for hydrogenase adsorbed on carbon filament material. The equilibrium hydrogen potential was achieved on mediatorless hydrogen enzyme electrodes in hydrogen atmosphere. The electrocatalytic activity of hydrogenase in direct bioelectrocatalysis of hydrogen oxidation was two orders of magnitude higher compared to platinum. The reported electrode remained 50% activity after 6 months of storage with periodical testing. Wired bioelectrocatalysis was achieved by adsorption of hydrogenase onto electropolymerized redox mediator N-methyl-N'-(12-pyrrol-1-yl-dodecyl)-4,4'-bipyridinium ditetrafluoroborate.
Morozov
S V
SV
Faculty of Chemistry, M.V. Lomonosov Moscow State University, Lenin Hills, 119899 Moscow, Russia. serge@enzyme.chem.msu.ru
Karyakina
E E
EE
Zorin
N A
NA
Varfolomeyev
S D
SD
Cosnier
S
S
Karyakin
A A
AA
D002384:Catalysis; D004563:Electrochemistry; D006864:Hydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D020616:Thiocapsa roseopersicina / Q000201:enzymology*
D006864:Hydrogenase