Vascular endothelial growth factor mRNA and its protein expression in decidua after terminating early pregnancy by mifepristone plus misoprostol.
OBJECTIVE
To investigate the action of mifepristone plus misoprostol on decidua at the level of vascular endothelial growth factor (VEGF) mRNA and its protein expression, as well as the mechanism of prolonged uterine hemorrhage after terminating early pregnancy with these drugs.


METHODS
Forty-five decidua specimens were obtained from 45 pregnant women with amenorrhea of 6-7 weeks' duration, in which 15 women were treated with mifepristone and 15 were given mifepristone plus misoprostol. Enzyme-linked immunosorbant assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect VEGF protein and mRNA levels in decidua.


RESULTS
In all three groups, only the VEGF121 amplification product of 452 bp was visualized. The mRNA and protein levels of VEGF showed no significant differences among the three groups (P > 0.05).


CONCLUSIONS
In humans, early decidua VEGF121 mRNA is the main isoform. The action of mifepristone plus misoprostol on blood vessels in human decidua may be medicated by some factors other than VEGF.
Huang
L
L
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. hlili@mail.hz.zj.cn
Shi
Y
Y
D000019:Abortifacient Agents / Q000494:pharmacology*; D000028:Abortion, Induced; D000328:Adult; D003656:Decidua / Q000737:chemistry / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000032:analysis / Q000235:genetics*; D015735:Mifepristone / Q000494:pharmacology*; D016595:Misoprostol / Q000494:pharmacology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000019:Abortifacient Agents; D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016595:Misoprostol; D015735:Mifepristone