Expression, mapping, and genetic variability of Brassica napus disease resistance gene analogues.
Numerous sequences analogous to resistance (R) genes exist in plant genomes and could be involved in resistance traits. The aim of this study was to identify a large number of Brassica napus sequences related to R genes and also to test the adequacy of specific PCR-based tools for studying them. Different consensus primers were compared for their efficiency in amplifying resistance-gene analogues (RGAs) related to the nucleotide-binding-site subgroup of R genes. Specific primers were subsequently designed to fine-study the different RGAs and we tested their efficiency in three species related to B. napus: Brassica oleracea, Brassica rapa, and Arabidopsis thaliana. Forty-four B. napus RGAs were identified. Among 29 examined, at least one-third were expressed. Eighteen RGAs were mapped on 10 of the 19 B. napus linkage groups. The high variability within these sequences permitted discrimination of each genotype within a B. napus collection. The RGA-specific primers amplified RGAs in the B. oleracea and B. rapa genomes, but the sequences appear to be poorly conserved in A. thaliana. Specific RGA primers are a precise tool for studying known-sequence RGAs. These sequences represent interesting markers that could be correlated with resistance traits in B. napus or related Brassica genomes.
Fourmann
M
M
Station de Génétique et Amelioration des Plantes, Institut National de la Recherche Agronomique, Versailles, France. fourmann@versailles.inra.fr
Chariot
F
F
Froger
N
N
Delourme
R
R
Brunel
D
D
D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D029688:Brassica napus / Q000235:genetics*; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D016415:Sequence Alignment