[Expression and characteristics of laryngeal carcinoma-associated antigen].
OBJECTIVE
To study the characteristics of laryngeal carcinoma-associated antigen (LCAA) and its expression on laryngeal carcinoma tissue.


METHODS
The binding of a cocktail monoclonal antibodies Lc9, Lc11, Lc12 to human laryngeal squamous-cell carcinoma, laryngeal precancerous lesions and normal laryngeal mucosa was examined by immunohistochemical method. LCAA isolated and purified by gel-filtration, and chromotography from 3 laryngeal squamous-cell carcinomas. The reactivity of the purified antigen was determined after treatment with trypsin, NaIO4, methanol and heating.


RESULTS
The positive rate of LCAA expression in 90 cases of laryngeal carcinoma, 14 cases of precancerous lesions and in 10 cases of normal laryngeal mucosa was 97.7%, 50.0% and 0, respectively. LCAA was a glycoprotein, moderately heat stable. The purified antigen gave two bands of apparent molecular weights of 61,400 and 56,500 on SDS-PAGE and Western blot.


CONCLUSIONS
The results provide a basis for immunoimaging diagnosis and targeting chemotherapy for laryngeal cancer.
Ni
Z
Z
Department of Otorhinolaryngology, Beijing Hospital, Beijing 100730.
Sun
L
L
Zhou
J
J
D000951:Antigens, Neoplasm / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000276:immunology*; D008970:Molecular Weight
D000951:Antigens, Neoplasm