title: Random chromosomal gene disruption using cassette mutagenesis.
authors.affiliation: Department of Applied Genomics, Bristol-Myers Squibb Pharmaceutical Research, Wallingford, CT, USA.
authors.forename: French A
authors.initials: FA
authors.lastname: Lewis
authors.forename: Brian A
authors.initials: BA
authors.lastname: Dougherty
mesh_terms: D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005658:Fungi / Q000235:genetics; D006193:Haemophilus influenzae / Q000235:genetics; D006602:Hexosyltransferases; D007202:Indicators and Reagents; D002267:Muramoylpentapeptide Carboxypeptidase / Q000737:chemistry / Q000235:genetics; D016254:Mutagenesis, Insertional; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; D002267:Muramoylpentapeptide Carboxypeptidase