Protection of intrinsic nerves of guinea-pig detrusor strips against anoxia/glucopenia and reperfusion injury by taurine.
There is ample evidence that ischaemia is associated with partial denervation of the detrusor muscle and that this is responsible for much of its abnormal contractile behaviour, resulting in bladder dysfunction (instability). In guinea-pig nerves are very susceptible to the ischaemic damage as compared to the muscle cells. The purpose of this study was to assess the neuroprotection afforded by taurine on guinea-pig detrusor under ischaemic-like conditions. Guinea-pig detrusor strips were subjected for 60 min to ischaemic-like conditions, followed by 150 min reperfusion. Intrinsic nerves underwent every 30 min electrical field stimulation (EFS) by 5-s trains of square voltage pulses of 0.05 ms duration (15 Hz, 50 V). Detrusor strips were perfused with 0.1, 1, 3 or 10 mM taurine during the ischaemia-like exposure and the first 30 min of reperfusion. Taurine (1 and 3 mM) significantly improved the response of the strips to EFS both at the end of ischaemia and reperfusion. On the contrary, neither 0.1 nor 10 mM taurine had significant effects. It is concluded that taurine can partially counteract the ischaemia-reperfusion injury in the guinea-pig urinary bladder.
Pessina
F
F
Istituto di Scienze Farmacologiche, Siena, Italia.
Matteucci
G
G
Esposito
L
L
Gorelli
B
B
Valoti
M
M
Sgaragli
G
G
D000615:Aminoethylphosphonic Acid / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects* / Q000502:physiology; D005947:Glucose; D006168:Guinea Pigs; D000860:Hypoxia; D007511:Ischemia; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000294:innervation* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010100:Oxygen; D058919:Purinergic P2 Receptor Antagonists; D015427:Reperfusion Injury / Q000378:metabolism*; D013498:Suramin / Q000494:pharmacology; D013654:Taurine / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology; D001743:Urinary Bladder / Q000294:innervation*
D018727:Muscarinic Antagonists; D018696:Neuroprotective Agents; D058919:Purinergic P2 Receptor Antagonists; D013654:Taurine; D013779:Tetrodotoxin; D013498:Suramin; D001285:Atropine; D000615:Aminoethylphosphonic Acid; D005947:Glucose; D010100:Oxygen