Comparison of endogenous feline leukemia virus RNA content in feline vaccine and nonvaccine site-associated sarcomas.
OBJECTIVE
To determine whether feline vaccine site-associated sarcomas (VSS) contain a higher amount of endogenous FeLV (enFeLV) RNA, compared with feline nonvaccine site-associated sarcomas (non-VSS).


METHODS
Formalin-fixed paraffin-embedded (FFPE) tissues from 50 VSS and 50 cutaneous non-VSS.


METHODS
RNA was extracted from FFPE sections of each tumor, and regions of the long terminal repeat (LTR) and envelope (env) gene of enFeLV were amplified by use of reverse transcriptase-polymerase chain reaction (RT-PCR). The density of each RT-PCR product band for enFeLV was compared with that of a constitutively expressed gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). An integrated density value (IDV) was determined by use of densitometry, and the IDV ratio for enFeLV to GAPDH was calculated for each enFeLV primer set.


RESULTS
The median (interquartile range) of the IDV ratio for the enFeLV LTR primer set was 0.52 (0.26 to 1.17) for the VSS group and 0.84 (0.21 to 1.53) for the non-VSS group. The median (interquartile range) of the IDV ratio for the enFeLV env primer set was 0.60 (0.37 to 0.91) for the VSS group and 0.59 (0.36 to 1.09) for the non-VSS group.


CONCLUSIONS
Because the amount of enFeLV RNA within the LTR and env gene was not significantly different between the VSS and non-VSS groups, enFeLV replication or expression is unlikely to be involved in VSS development.
Kidney
B A
BA
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Ellis
J A
JA
Haines
D M
DM
Jackson
M L
ML
D000818:Animals; D002371:Cat Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology*; D002415:Cats; D003720:Densitometry; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005354:Fibrosarcoma / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D005256:Leukemia Virus, Feline / Q000737:chemistry / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric; D014611:Vaccination / Q000009:adverse effects / Q000662:veterinary*
D012367:RNA, Viral