Reagentless biosensing using electrochemical impedance spectroscopy.
The use of electrochemical impedance spectroscopy (EIS) and the conducting polymer, poly (pyrrole), as an integrated recognition and transduction system for reagentless biosensor systems was demonstrated with two different systems. The first system being an immunoassay for detection of luteinising hormone (LH) with the antibody being entrapped with in the poly (pyrrole) matrix and the second, a construct for DNA hybridisation discrimination able to differentiate single- and double-stranded DNA based on the interaction of the DNA with poly (pyrrole).
Farace
G
G
IRC in Biomedical Materials, Queen Mary and Westfield College, University of London, Mile End Road, London E1 4NS, UK. g.farace@qmw.ac.uk
Lillie
G
G
Hianik
T
T
Payne
P
P
Vadgama
P
P
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D007986:Luteinizing Hormone / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*
D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D007986:Luteinizing Hormone; D004247:DNA