Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites.
Chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI, or NR2F1) is an orphan nuclear receptor that plays a major role in the development of the nervous system. We show here that three ETS response elements in the COUP-TFI promoter mediate its transcription. A reporter gene containing these ETS binding sites is activated by Ets-1, while the same reporter with point mutations on all three ETS response elements is not. We also show that Ets-1 binds to these response elements and that other ETS factors also transactivate the COUP-TFI promoter. In addition, COUP-TFI is coexpressed with some ETS factors in the mouse embryo. These results indicate that members of the ETS family can activate COUP-TFI gene expression.
Salas
Ramiro
R
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Petit
Fabrice G
FG
Pipaon
Carlos
C
Tsai
Ming-Jer
MJ
Tsai
Sophia Y
SY
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D004622:Embryo, Mammalian / Q000737:chemistry; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000502:physiology*; D050783:Proto-Oncogene Proteins c-ets; D020218:Response Elements; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D015533:Transcriptional Activation
D051838:COUP Transcription Factor I; D004268:DNA-Binding Proteins; C494041:ETS1 protein, human; C494042:Ets1 protein, mouse; C497589:NR2F1 protein, human; C497590:Nr2f1 protein, mouse; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors