Electrogenic properties of the Na+,K+-ATPase probed by presteady state and relaxation studies.
Electrical measurements on planar lipid bilayers, patch/voltage clamp experiments, and spectroscopic investigations involving a potential sensitive dye are reviewed. These experiments were performed to analyze the kinetics of charge translocation of the Na+,K+-ATPase. High time resolution was achieved by applying caged ATP, voltage-jump, and stopped-flow techniques, respectively. Kinetic parameters and the electrogenicity of the relevant transitions in the Na+,K+-ATPase reaction cycle are discussed.
Bamberg
E
E
Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany. ernst.bamberg@mpibp-frankfurt.mpg.de
Clarke
R J
RJ
Fendler
K
K
D004553:Electric Conductivity; D004594:Electrophysiology / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D018408:Patch-Clamp Techniques; D011726:Pyridinium Compounds / Q000032:analysis; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013343:Styrenes / Q000032:analysis
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D013343:Styrenes; C070534:RH 421; D000254:Sodium-Potassium-Exchanging ATPase