[Effect of platelet on procoagulant activity of monocyte].
OBJECTIVE
To investigate the effect of platelet on procoagulant activity of mononcyte(M).


METHODS
The procoagulant activity of M lysates was measured by one stage clotting time assay, the tissue factor (TF) activity and the role of P-selectin were confirmed by specific monoclonal antibodies (McAb).


RESULTS
1. Activated M challenged by lipopolysaccharide(LPS) had augmented procoagulant activity and this effect was blocked by TF McAb. 2. Procoagulant activity of M and activated M increased significantly with the presence of activated platelets. 3. The enhancement effect of activated platelet on the procoagulant activity of M was blocked by P-selectin McAb.


CONCLUSIONS
TF expression of M contributes to the procoagulant activity of Ms and activated platelet enhances the procoagulant activity via P-selectin.
Xu
J
J
Hemostatic Physiology Laboratory, Xiangya Medical College, Central Southern University, Changsha 410078, China.
He
X
X
Xiong
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001779:Blood Coagulation Factors / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D019007:P-Selectin / Q000276:immunology / Q000378:metabolism*; D013925:Thromboplastin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D008070:Lipopolysaccharides; D019007:P-Selectin; C045071:leukocyte procoagulant activity; D013925:Thromboplastin