title: Stabilization of ferrocene leakage by physical retention in a cellulose acetate membrane. The fructose biosensor.
abstract: The prevention of ferrocene leakage from an electrode by physical retention of mediator in a matrix of cellulose acetate membrane is reported. Five types of the cellulose acetate membranes were prepared, containing 1.8%, 5.3%, 8.5%, 20.0% of ferrocene and a membrane containing 1.8% of ferrocene and 0.05 % of Nafion in the matrix. Ferrocene embedded membranes were successfully applied in the construction of a fructose biosensor by immobilization of PQQ-dependent fructose dehydrogenase (FDH). The biosensor comprising a cellulose acetate membrane with 1.8% of ferrocene and 0.05% of Nafion had good stability characteristics, retained almost 40% of the initial response after 8 h of continuous use with an initial sensitivity of 226 nA mM(-1) and response time of 75 s.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-842 38 Bratislava, Slovak Republic. chemtkac@savba.sk
authors.forename: Ján
authors.initials: J
authors.lastname: Tkác
authors.forename: Igor
authors.initials: I
authors.lastname: Vostiar
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.forename: Ernest
authors.initials: E
authors.lastname: Sturdík
mesh_terms: D015374:Biosensing Techniques; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D005296:Ferrous Compounds / Q000737:chemistry*; D005632:Fructose / Q000737:chemistry*; D008567:Membranes, Artificial; D000075163:Metallocenes
chemical_list: D005296:Ferrous Compounds; D008567:Membranes, Artificial; D000075163:Metallocenes; D005632:Fructose; C005062:acetylcellulose; D002482:Cellulose; C004998:ferrocene