[Relationship between elastase activity and leukocyte counts in gingival crevicular fluid].
OBJECTIVE
To determine the relationship among the elastase(EA) activity in the supernatant (EA-s), and in the pellet (EA-p) after centrifuge, and polymorphonuclear leukocyte (PMN) counts in gingival crevicular fluid.


METHODS
Gingival crevicular fluid (GCF) was sampled with filter paper strips by intra-pocket method to determine EA levels and then with gingival crevicular washings after 24 hours at the same site for PMN counting. The levels of EA-s and EA-p were determined seperately using the subtract Meosuc-ala-ala-val-pro-NA. PMNs at the same site were counted with light microscope.


RESULTS
The research demonstrated significantly positive correlation among EA-s and EA-p per site and the PMN counts in GCF (P < 0.001).


CONCLUSIONS
GCF-EA-p per site could reflect the amount of PMNs collected from the pockets, and EA can be the marker of PMN in GCF.
He
L
L
School of Stomatology, Beijing Medical University, Beijing 100081.
Cao
C
C
Sha
Y
Y
D000328:Adult; D005260:Female; D005883:Gingival Crevicular Fluid / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D010196:Pancreatic Elastase / Q000378:metabolism*
D010196:Pancreatic Elastase