[ATPase activity of Neisseria gonorrhoeae and Staphylococcus aureus strains].
It has been established that membrane vesicles of gonococci possess ATPase activity within 0.05-0.14 mmol of ATP during 60 min per 1 mg of protein and for staphylococcus--within 0.07-0.19 mmol of ATP for 60 min per 1 mg of protein. The identity in all the kinetic parameters is observed for all the studied gonococcus strains: Na+ and Ca2+ ions inhibit ATPase activity within 12-19%; Mg2+ ions increase the ATPase activity 2-2.5 times. Dicyclohexylcarboimide, a specific ATPase inhibitor, suppresses ATPase activity by 50-70%. ATPase activity in the strains of bacteria containing antibiotic-resistant plasmids is established to increase 1.2-2.8 times for gonococcus and 1.2-2.7 for staphylococcus as compared to sensitive ones.
Skliar
T V
TV
Dniepropetrovsk National University, 13 Naukovy Lane, Dniepropetrovsk, 49050, Ukraine.
Krysenko
A V
AV
Kozitskaia
S N
SN
Gavriliuk
V G
VG
Vinnikov
A I
AI
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology*
D004791:Enzyme Inhibitors; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases
ATFaznaia aktivnost' shtammov Neisseria gonorrhoeae i Staphylococcus aureus.