title: Immunodominant membrane proteins from two phytoplasmas in the aster yellows clade (chlorante aster yellows and clover phyllody) are highly divergent in the major hydrophilic region.
abstract: The mechanisms by which phytoplasmas interact with their hosts are not understood. Mollicute membrane proteins may play a role in such interactions and therefore the amp genes encoding immunodominant proteins from two phytoplasmas, aster yellows and clover phyllody, which fall within the largest taxonomic subclade of the phytoplasmas, have been cloned and characterized. The putative translation products, antigenic membrane proteins (Amps), of these genes have properties which are typical for bacterial membrane proteins, and which suggest that each has a single large extracellular hydrophilic domain held by a transmembrane region near the C-terminus, with only a short C-terminal intracellular sequence. Both of the Amps characterized here have bacterial leader sequences which are cleaved during maturation. Whilst the signal peptide and transmembrane regions of the two proteins are very similar, the major hydrophilic domains are highly divergent in both size and sequence. The Amps from the two phytoplasmas are also different in structure and sequence from the immunodominant membrane proteins of three other phytoplasmas whose genes have been cloned previously.
authors.affiliation: Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK1.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Barbara
authors.affiliation: Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK1.
authors.forename: Anne
authors.initials: A
authors.lastname: Morton
authors.affiliation: Entomology and Plant Pathology, Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, UK2.
authors.forename: Michael F
authors.initials: MF
authors.lastname: Clark
authors.affiliation: Entomology and Plant Pathology, Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, UK2.
authors.forename: David L
authors.initials: DL
authors.lastname: Davies
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D029902:Medicago / Q000382:microbiology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D008973:Tenericutes / Q000235:genetics / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D016056:Immunodominant Epitopes