[Study of loss of heterozygosity at chromosome 11q13 in nasopharyngeal carcinoma].
OBJECTIVE
To analyze the loss of heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC).


METHODS
Relatively pure tumor tissues were obtained by microdissetion. PCR was used to perform microsatellite analysis of NPC samples using D11S4946, PYGM, D11S449 and INT-2 as primers, which span the MEN-1 gene.


RESULTS
Thirty-six of 38 cases studied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (78.8%). The LOH frequency was 51.5% for INT-2, 45.5% for PYGM and 45.7% for D11S449, respectively.


CONCLUSIONS
The high frequency of loss of heterozygosity at 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.
Guo
Y
Y
Department of Etiology, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Fang
Y
Y
Huang
B
B
D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm