[The avian peripheral olfactory system: model for study of apoptosis and cellular regeneration].
A reliable model, usable in vitro and in vivo, is necessary for analysis of processes engaged during cell death, regeneration and differentiation. The peripheral olfactory system is an attractive model for studying these processes through its dynamic neurogenesis that occurs continually throughout the lifetime.


OBJECTIVE
The aim of this study was the analysis of these processes on an animal model.


METHODS
We performed axotomy of the nerve olfactory on young animals and chicken embryos E17. Then we infused IGF-I (insulin-like growth factor-I) in the lesioned site. Death, regeneration and differentiation of cells were studied by immunocytology.


RESULTS
After hatching, the section of the olfactory nerve induced a rapid neuronal apoptosis at the 24th hour followed by a wave of mitosis 24 hours later. In prenatal stages, the response to the axotomy was rather similar to a dedifferentiation. In postnatal stages, the IGF-I infusion at the lesioned site had a triple function: survival of mature neurons, maintenance of differentiation and stimulation of mitosis. The neoneurogenesis, which occurred from neuronal stem cells would depend on the maturation and environment of the olfactory neurons protected from apoptosis by IGF-I.


CONCLUSIONS
The avian olfactory epithelium is a good model for analysis of cell death, regeneration and differentiation. The capacity of these neuronal stem cells to dedifferentiate makes then more primitive than the pluripotent cells, closer to totipotent embryonic stem cells.
Mathonnet
M
M
Service de chirurgie digestive endocrinienne et générale, CHU Dupuytren, 2, avenue Martin-Luther-King, 87 042 Limoges, France. mathonnet@unilim.fr
Cubertafond
P
P
Gainant
A
A
Ayer-Le Lièvre
C
C
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002468:Cell Physiological Phenomena; D002642:Chick Embryo; D002645:Chickens; D004847:Epithelial Cells / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D023421:Models, Animal; D009416:Nerve Regeneration; D009832:Olfactory Nerve / Q000166:cytology* / Q000502:physiology
D007334:Insulin-Like Growth Factor I
Le système olfactif périphérique aviaire: modèle d'étude de l'apoptose et de la régénération cellulaire.