[Study of APC, Rb, c-met gene copy numbers of human gastric mucosa epithelial cell line GES-1].
OBJECTIVE
To study the copy number of oncogene/tumor suppressor genes in GES-1 cell line to identify its characteristics.


METHODS
Bio-14-dATP was incorporated into APC, Rb, c-met gene cloned in plasmid by nick translation. Fluorescence in situ hybridization (FISH) of interphase nuclei of GES-1 cells was performed.


RESULTS
Two copies of APC were shown in 48% interphase nuclei and 3 copies in 22%; 71% and 80% cells had normal copies of Rb and c-met genes in their nuclei, respectively.


CONCLUSIONS
GES-1 cell line is a relatively normal gastric mucosa epithelial cell line and can be used as a human in vitro model system for the study of carcinogenesis.
Zheng
S
S
Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University, Beijing 100034.
Ke
Y
Y
D002460:Cell Line; D005753:Gastric Mucosa / Q000378:metabolism*; D018628:Gene Dosage; D017491:Genes, APC; D016161:Genes, Retinoblastoma; D006801:Humans; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics*; D011519:Proto-Oncogenes
D019859:Proto-Oncogene Proteins c-met