title: Stage-specific transcription of distinct repertoires of a multigene family during Plasmodium life cycle.
abstract: Members of a multigene family in the rodent malaria parasite Plasmodium yoelii yoelii code for 235-kilodalton proteins (Py235) that are located in the merozoite apical complex, are implicated in virulence, and may determine red blood cell specificity. We show that distinct subsets of py235 genes are expressed in sporozoites and hepatic and erythrocytic stages. Antibodies to Py235 inhibited sporozoite invasion of hepatocytes. The switch in expression profile occurred immediately after transition from one stage to another. The results suggest that this differential expression is driven by strong biological requirements and provide evidence that hepatic and erythrocytic merozoites differ.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, The Ridgeway, London, NW7 1AA, UK. ppreise@nimr.mrc.ac.uk
authors.forename: P R
authors.initials: PR
authors.lastname: Preiser
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: F T M
authors.initials: FT
authors.lastname: Costa
authors.forename: W
authors.initials: W
authors.lastname: Jarra
authors.forename: E
authors.initials: E
authors.lastname: Belnoue
authors.forename: S
authors.initials: S
authors.lastname: Ogun
authors.forename: A A
authors.initials: AA
authors.lastname: Holder
authors.forename: T
authors.initials: T
authors.lastname: Voza
authors.forename: I
authors.initials: I
authors.lastname: Landau
authors.forename: G
authors.initials: G
authors.lastname: Snounou
authors.forename: L
authors.initials: L
authors.lastname: Rénia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000469:parasitology; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017125:Genes, Protozoan; D022781:Hepatocytes / Q000469:parasitology; D008018:Life Cycle Stages; D008288:Malaria / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D005810:Multigene Family; D010967:Plasmodium yoelii / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012469:Salivary Glands / Q000469:parasitology; D014158:Transcription, Genetic
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger