Inhibition of apoptosis by primary isolates of herpes simplex virus.
HSV-1 inhibits apoptosis of the infected cell, presumably to increase viral yield. Weaker anti-apoptotic activity was previously reported in laboratory-adapted HSV-2, but not in two low-passage clinical HSV-2 isolates, suggesting that the anti-apoptotic effect might result from adaptation to laboratory growth. We therefore assembled a large panel of clinical strains of HSV-1 and HSV-2. Clinical and laboratory strains of HSV-1 strongly inhibited apoptosis of Jurkat cells, as determined by morphologic change or caspase activation, while clinical HSV-2 strains showed no inhibitory activity in Jurkat cells. These findings suggest fundamental differences between HSV-1 and HSV-2 in their manipulation of host cell apoptosis.
Jerome
K R
KR
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA. kjerome@u.washington.edu
Fox
R
R
Chen
Z
Z
Sarkar
P
P
Corey
L
L
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000502:physiology; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000502:physiology*; D018258:Herpesvirus 2, Human / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D014709:Vero Cells
D020169:Caspases