Role of placental alkaline phosphatase in the interaction between human placental trophoblast and Trypanosoma cruzi.
Congenital Chagas disease, due to the intracellular parasite Trypanosoma cruzi, is associated with premature labor, miscarriage, and placentitis. Human enzyme placental alkaline phosphatase (PLAP) (EC 3.1.3.1.) is membrane-anchored through glycosylphosphatidylinositol (GPI). PLAP is present in plasma in late pregnancy, 36 to 40 weeks; there are lower levels in maternal Chagas disease. Infants born to such mothers may have congenital Chagas disease. Human placental villi (PV) were treated with phospholipase-C (PL-C) and then cultured with T. cruzi to determine the effect of the parasites on PLAP activity as an in vitro model. There is less PLAP activity after treatment by PL-C and during culture with T. cruzi. Pretreatment of PV with PL-C before culture with T. cruzi yielded essentially normal specific activity of PLAP and prevented or greatly reduced infective penetration of villi by parasites. The results are consistent with a pathogenetic role for placental alkaline phosphatase in congenital Chagas disease. Receptor activation of membrane attachment to PLAP may be a device used by T. cruzi to enable parasite invasion of human trophoblast.
Sartori
M J
MJ
Ila. Cátedra de Histología, Embriología y Genética, Universidad Nacional de Córdoba, Córdoba, Argentina. msartori@cmefcm.uncor.edu
Lin
S
S
Frank
F M
FM
Malchiodi
E L
EL
de Fabro
S P
SP
D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D014355:Chagas Disease / Q000151:congenital* / Q000201:enzymology / Q000503:physiopathology; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010920:Placenta / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000469:parasitology*; D011247:Pregnancy; D014327:Trophoblasts / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D000469:Alkaline Phosphatase; D010738:Type C Phospholipases