Multisite phosphorylation and the countdown to S phase.
Remarkably, SCF(Cdc4) ubiquitin ligase binds and ubiquitinates Sic1 decorated with six, but not five, phosphates. This numerical wizardry suggests how analog inputs can be rectified to digital outputs. Unraveling the counting mechanism promises to generate new insights into the architecture of protein nanoprocessors.
Deshaies
R J
RJ
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. deshaies@its.caltech.edu
Ferrell
J E
JE
D000818:Animals; D018797:Cell Cycle Proteins / Q000502:physiology; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D018844:Cyclin-Dependent Kinases / Q000502:physiology; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; D005656:Fungal Proteins / Q000502:physiology; D006801:Humans; D010453:Peptide Synthases / Q000502:physiology; D010766:Phosphorylation; D016196:S Phase / Q000502:physiology*; D044843:SKP Cullin F-Box Protein Ligases; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases
D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; D005656:Fungal Proteins; C086926:SIC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D044843:SKP Cullin F-Box Protein Ligases; D044767:Ubiquitin-Protein Ligases; D018844:Cyclin-Dependent Kinases; D010453:Peptide Synthases