title: Transplantation of human fetal brain cells into ischemic lesions of adult gerbil hippocampus.
abstract: OBJECTIVE
  The goal of this study was to establish whether transplanted cells derived from fetal human brain can survive in an ischemic lesion.
  
  
  METHODS
  Sixteen adult male Mongolian gerbils underwent transient bilateral common carotid artery occlusion. One week later, cell suspensions prepared from fetal human brain were injected using stereotactic guidance into the CA1 region of the hippocampus on one side. On the contralateral side injection of the cell suspension medium only was performed. One week after transplantation, the animals were perfusion fixed and their brains were processed for histological studies as well as expression of neuron and glia-specific antigens. Data from ischemic animals were compared with eight nonischemic gerbils that served as sham-operated controls. Last, the in vivo data were correlated with observations made from matching in vitro cultures of the fetal brain cell suspension. The in vivo data indicated that transplanted human fetus-derived brain cells survived in ischemic lesions of gerbil hippocampus after 1 week, provided that the host animal underwent adequate immunosuppression and the transplanted cells were not incorporated into the scar caused by the transplantation procedure. Unlike their in vivo counterparts, after 1 week, most cultured fetal brain cells expressed either neuron- or astrocyte-specific antigens.
  
  
  CONCLUSIONS
  This work demonstrates that xenotransplanted fetal human brain cells are able to survive in an ischemic lesion in a rodent model. These data might be useful for future neural transplantation studies of treatments for cerebrovascular ischemia in humans.
authors.affiliation: Department of Neurosurgery, Wayne State University, Detroit, Michigan 48201, USA. kbarami@neurosurgery.wayne.edu
authors.forename: K
authors.initials: K
authors.lastname: Barami
authors.forename: H N
authors.initials: HN
authors.lastname: Hao
authors.forename: G A
authors.initials: GA
authors.lastname: Lotoczky
authors.forename: F G
authors.initials: FG
authors.lastname: Diaz
authors.forename: W D
authors.initials: WD
authors.lastname: Lyman
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology* / Q000628:therapy*; D016380:Brain Tissue Transplantation; D016893:Carotid Stenosis / Q000150:complications; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D004195:Disease Models, Animal; D016332:Fetal Tissue Transplantation; D005849:Gerbillinae; D006624:Hippocampus / Q000098:blood supply / Q000473:pathology*; D006801:Humans; D008297:Male; D013238:Stereotaxic Techniques; D014183:Transplantation, Heterologous / Q000473:pathology