No factors except for the hap complex increase the Taka-amylase A gene expression by binding to the CCAAT sequence in the promoter region.
To examine the possibility that factors different from the Hap complex are involved in increasing Taka-amylase A gene (taa) expression in Aspergillus nidulans, either the authentic taa gene or the mutant taa gene with a replacement of the CCAAT box was expressed in either a wild type strain or a mutant strain lacking the functional Hap complex (hapCdelta). When the mutant taa was expressed in the hapCdelta strain, enzyme activity was as low as that of the hapCdelta strain transformed with the authentic taa gene, indicating that no factors except for the Hap complex increase transcription of the taa gene by binding to the CCAAT sequence.
Kato
M
M
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan. kato@agr.nagoya-u.ac.jp
Naruse
F
F
Kobayashi
T
T
Tsukagoshi
N
N
D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic / Q000235:genetics; D000516:alpha-Amylases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
D023081:CCAAT-Binding Factor; D005656:Fungal Proteins; C100785:HapC protein, Emericella nidulans; D000516:alpha-Amylases