[Effects of Astragaloside in treating myocardial injury and myocardial Sarco/Endoplasmic Ca(2+)-ATPase of viral myocarditis mice].
OBJECTIVE
To investigate the effects on myocardial injury and sarcoplasmic reticulum (SR) Ca(2+)-ATPase of viral myocarditis mice treated with Astragaloside (AS) and Astragalus Injection (AI).


METHODS
Viral myocarditis model was created by intraperitoneal inoculation with coxsackievirus B3m (CVB3m) solution and were divided into model, AS, AI and normal control groups. The mortality, myocardial pathological changes, serum cardiac troponin I (cTnI) and the activity of myocardial Sarco/Endoplasmic Ca(2+)-ATPase (SERCA) were observed.


RESULTS
The mortality of model was higher than that of the normal control (P = 0.0042), AS and AI (P < 0.05). The serum level of cTnI of model was significantly higher than that of the normal control (P < 0.001), AS (P < 0.025) and AI (P < 0.05). The myocardial necrosis and inflammatory changes of AS and AI groups were alleviated than that of model (P < 0.01). The activity of myocardial SERCA of model were significantly lower than that of normal control (P < 0.001), AS (P < 0.01) and AI (P < 0.05).


CONCLUSIONS
AS and AI have some protecting effects on myocardial injury of viral myocarditis mice. AS is the effective component of Astragalus membranaceus in treating viral myocarditis. One of the mechanisms of Astragalus membranaceus and AS for viral myocarditis mice depriving of the myocardial injury may be due to improve the activity of myocardial SERCA in the mice.
Lu
S
S
Institute of Cardiovascular Diseases, First Affiliated Hospital, Nanjing Medical University, Nanjing (210029).
Zhang
J
J
Yang
D
D
D000818:Animals; D027883:Astragalus Plant; D027885:Astragalus propinquus; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003384:Coxsackievirus Infections / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D029822:Enterovirus B, Human; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000473:pathology* / Q000821:virology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D019210:Troponin I / Q000378:metabolism
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D012503:Saponins; D019210:Troponin I; C027492:Huang Qi; D000252:Calcium-Transporting ATPases