Fish cytogenetics and the future of radiation biodosimetry.
Fluorescence in situ hybridisation (FISH) with whole chromosome paints has greatly facilitated the analysis of structural chromosome aberrations and has led to translocations replacing dicentrics as the aberration of choice for many applications. Major challenges remain if we are to go from translocations to an understanding of the health consequences of radiation exposure. Yet to be surmounted are the roles of individual susceptibility, time since exposure, and the effects of subjects age. Accomplishing these objectives will require automation, reduced costs, improved calibration, and extensive use of baseline samples.
Tucker
J D
JD
Biology and Biotechnology Research Program Lawrence Livermore National Laboratory PO Box 808, L-448, 7000 East Ave. Livermore, CA 94550, USA. tucker5@llnl.gov
D020732:Cytogenetic Analysis; D005544:Forecasting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011829:Radiation Dosage; D011874:Radiometry; D012680:Sensitivity and Specificity; D014178:Translocation, Genetic / Q000502:physiology*