Identification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences.
A highly specific and reproducible approach for the simultaneous detection of enteric pathogenic bacteria was developed using bacterial hsp60 gene and molecular biological tools. A single pair of universal primers was derived from the highly conserved sequence of hsp60 genes encompassing a 600-bp hypervariable region. PCR amplification followed by either dot blot hybridization or restriction enzyme digestion performed on 38 enteric bacteria indicated that this approach could differentiate not only different genera such as Campylobacter, Yersinia and Vibrio, but also species that are closely related genetically, such as between C. jejuni and C. coli, or between Salmonella and Shigella or Escherichia coli.
Wong
Rebecca S Y
RS
Division of Infectious Diseases, Vancouver Hospital Health Sciencs Center, BC, Canada.
Chow
Anthony W
AW
D000595:Amino Acid Sequence; D018834:Chaperonin 60 / Q000235:genetics*; D003967:Diarrhea / Q000175:diagnosis / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018834:Chaperonin 60