[Effect of emodin on human kidney fibroblast proliferation].
OBJECTIVE
To study the effect of emodin on proliferation of human kidney fibroblasts in vitro.


METHODS
After human kidney fibroblasts were cultured, isolated and identified, both the effects of five different concentrations of emodin (10, 30, 50, 80 and 100 micrograms/ml) on 3H-TdR incorporation, and the effects of three different concentrations of emodin (10, 50 and 100 micrograms/ml) on cell cycle by flow cytometry were investigated.


RESULTS
The exposure of human kidney fibroblasts to emodin (10-100 micrograms/ml) caused a dose-dependent reduction in 3H-TdR (r = 0.995, P < 0.01), and could delay the progress of human kidney fibroblasts from G1 to S phase.


CONCLUSIONS
Emodin inhibited the proliferation of human kidney's fibroblasts by inhibiting the cell DNA synthase and delaying the progress of cell cycle. These findings might provide part of experimental basis for the clinical use of emodin.
Ning
Y
Y
Second Affiliated Hospital of Lanzhou Medical College, Lanzhou (730030).
Wang
J
J
Qu
S
S
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004642:Emodin / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007668:Kidney / Q000166:cytology*
D004642:Emodin