title: Stable clonal expansion of the T-cell receptor V beta 6, V beta 17 and V beta 19 T cells in a cGVHD case using genescan analysis.
abstract: OBJECTIVE
  To investigate the distribution and clonality of the T-cell receptor (TCR) V beta repertoire in chronic graft versus host disease (cGVHD).
  
  
  METHODS
  The complementarity determining region 3 (CDR3) of the TCR beta gene with 24 variable regions was amplified in peripheral blood mononuclear cells drawn from one cGVHD patient after allogenic bone marrow transplantation (allo-BMT) 35, 39, 43 or 45 months respectively, using RT-PCR, to observe the expression of TCR V beta repertoire T cells. The PCR products were further analyzed by genescan to evaluate clonality of T cells.
  
  
  RESULTS
  Fourteen or 16 TCR V beta subfamily T cells were detected in each sample of cGVHD case. Oligoclonal T cells were identified in TCR V beta 6, 16, 17, 19 and 21 subfamilies. The stable clonal T cells in all samples were identified in V beta 6, V beta 17 and V beta 21 subfamilies.
  
  
  CONCLUSIONS
  Skewing distribution and stable clonal expansion of T cells can be found in cGVHD cases and it may be related to the initiation of cGVHD.
authors.affiliation: Department of Hematology, Medical College, Jinan University, Guangzhou 510632, China. jnyanqiuli@163.net
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Du
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D000328:Adult; D002908:Chronic Disease; D019674:Genes, T-Cell Receptor beta; D006086:Graft vs Host Disease / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*