Photolithographic generation of protein micropatterns for neuron culture applications.
Standard positive photoresist techniques were adapted to generate micropatterns of proteins on glass and oxide surfaces. Both lift-off and plasma-etching techniques were used to transfer the photoresist pattern into a layer of covalently immobilised protein. The surface properties of the areas adjacent to the patterns were altered by chemical surface modification. Using a combination of the lift-off and the etching process complementary patterns of two different proteins were generated. The biochemical and biological functionality of the protein patterns were assessed by immunostaining and by investigating the outgrowth of neurites from neurons plated on the patterned substrates. The investigated patterning processes are compatible with microstructuring and thin film processes, and may be used to generate functional surfaces for sensor and neuron culture applications.
Sorribas
Helga
H
Laboratory for Micro- and Nanotechnology, Paul Scherrer Institut, Villigen, Switzerland.
Padeste
Celestino
C
Tiefenauer
Louis
L
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal; D018929:Cell Culture Techniques / Q000379:methods; D002642:Chick Embryo; D058969:Contactin 2; D005727:Ganglia, Spinal / Q000166:cytology; D005898:Glass; D007074:Immunoglobulin G; D009474:Neurons / Q000166:cytology*; D009842:Oligopeptides; D011817:Rabbits; D019809:Streptavidin; D013499:Surface Properties
D015816:Cell Adhesion Molecules, Neuronal; D058969:Contactin 2; D007074:Immunoglobulin G; D009842:Oligopeptides; D019809:Streptavidin