title: Relationship between c-fos gene expression and delayed neuronal death in rat neonatal hippocampus following hypoxic-ischemic insult.
abstract: OBJECTIVE
  To explore the mechanism of perinatal hypoxia-ischemia encephalopathy, we studied the expression of the c-fos gene and its relationship with delayed neuronal death in a rat model.
  
  
  METHODS
  Cerebral hypoxia-ischemia was produced in 7-day-old SD rats using the Rice model. Reverse transcription PCR (RT-PCR), immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) were used to detect the expression of c-fos gene and cell apoptosis in the hippocampus.
  
  
  RESULTS
  The selective expression of c-fos and delayed cell apoptosis were observed in the hypoxia-ischemia hippocampus. Expression of the c-fos gene was seen in the CA4 and cingulate sulcus neurons, and apoptosis was observed in the CA1 neurons.
  
  
  CONCLUSIONS
  Transient expression of the c-fos gene may induce cerebral cell apoptosis, and may have complex relations with delayed cell death.
authors.affiliation: Department of Pediatrics, Affiliated Hospital, Nanjing Railway Medical College, Nanjing 210009, China.
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D016762:Genes, fos; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger