[Study on combined detection of M. genitalium, M. pneumoniae and C. pneumonia by nested polymerase chain reaction in respiratory tract infections in pediatric patients].
OBJECTIVE
To study the respiratory tract infections by Mg, M. genitalium (Mpn), Cpn, and co-infections by these agents in pediatric patients.


METHODS
Mg DNA, Mpn DNA and Cpn DNA were detected in respiratory tract specimens obtained by throat swab from 231 children by the nested polymerase chain reaction (PCR) technique. DNA sequencings were detected and analysed between every two positive specimens of Mg and Cpn and typical strains.


RESULTS
Positive rates of Mg DNA, Mpn DNA and Cpn DNA by nested PCR in 231 children were 22.5%, 30.7% and 32.9%. The Positive rates of co-infection were 5.2% (Mg and Mpn), 2.6% (Mg and Cpn), 10.8% (Mpn and Cpn) and 8.7% (Mg and Mpn and Cpn). There was only one base mutation in 1 Mg DNA. Other DNA sequencings were same as those from model strains. The homogeneity was 99.8%.


CONCLUSIONS
Mg could be one of the pathogens in respiratory tract infections in pediatric patients. There exists co-infection by Mg, Mpn and Cpn.
Lu
Y
Y
Third Hospital of Su Zhou Medical College, Changzhou 213003.
Meng
P
P
Ding
Y
Y
D002648:Child; D002690:Chlamydia Infections / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000382:microbiology*; D009177:Mycoplasma pneumoniae / Q000235:genetics / Q000302:isolation & purification; D018410:Pneumonia, Bacterial / Q000382:microbiology*; D011019:Pneumonia, Mycoplasma / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012141:Respiratory Tract Infections / Q000382:microbiology
D004269:DNA, Bacterial