title: [Serial cultivation of normal human oral keratinocytes].
abstract: OBJECTIVE
  To establish a method of culturing normal human oral keratinocytes.
  
  
  METHODS
  Specimens obtained from healthy humans undergoing oral surgery were dissociated by Dispase and trypsin into a single cell suspension. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope (EM). Cytokeratins were showed by immunohistochemistry.
  
  
  RESULTS
  The cells could be maintained in culture up to 4-5 passages, or 30-50 days. EM revealed that there were desmosomes and tonofibrils in the culture cells. The cells showed positive staining for cytokeratin antibody.
  
  
  CONCLUSIONS
  The culture technique for growing normal human oral keratinocytes has been established.
authors.affiliation: Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghan 200011, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Shang
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D008875:Middle Aged; D009061:Mouth Mucosa / Q000502:physiology*