Iminodiacetate and nitrilotriacetate degradation by Kluyveromyces marxianus IMB3.
The thermotolerant yeast Kluyveromyces marxianus IMB3 was capable of utilising either iminodiacetate or nitrilotriacetate as a sole source of nitrogen for growth. Cell extracts contained iminodiacetate dehydrogenase and nitrilotriacetate monooxygenase activities, suggesting the presence in the yeast of orthologues of these bacterial enzymes. The activities were not detectable in complete medium-growth cells, nor in nitrogen-starved cells, suggesting an inducible biodedgradation pathway for biodegradation of these xenobiotics, which has not been previously reported in a eukaryotic cell system. This observation emphasises the hitherto unrealised importance of yeast strains in the biodegradation of xenobiotics in the environment.
Ternan
Nigel G
NG
School of Biological and Environmental Sciences, University of Ulster, Coleraine County, Londonderry, BT52 1SA, United Kingdom. ng.ternan@ulst.ac.uk
McMullan
Geoffrey
G
D001673:Biodegradation, Environmental; D002455:Cell Division / Q000187:drug effects; D002457:Cell Extracts / Q000737:chemistry; D004790:Enzyme Induction / Q000187:drug effects; D007098:Imino Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D007716:Kluyveromyces / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009571:Nitrilotriacetic Acid / Q000378:metabolism* / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism; D013778:Tetrazolium Salts / Q000737:chemistry
D002457:Cell Extracts; D007098:Imino Acids; D013778:Tetrazolium Salts; C013574:iodonitrotetrazolium; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D009571:Nitrilotriacetic Acid; C008109:iminodiacetic acid