title: [Association between type 1 diabetes and polymorphism of the CTLA-4 gene in a Tunisian population].
abstract: Susceptibility to type 1 diabetes mellitus is strongly associated with particular HLA class II alleles. However, non HLA genetic factors are likely to be required for the development of disease. The candidate genes include the cytotoxic T lymphocyte associated 4 (CTLA-4) located on chromosome 2q33 and designated (IDDM12), which encodes a cell surface negative signal T molecule providing for activation. We investigated CTLA-4 exon 1 dimorphism in 74 type 1 patients and a control group of 48 healthy subjects from Tunisia using two methods PCR (polymerase chain reaction) allele specific and polymerase chain reaction restriction fragment length polymorphism (PCR RFLP). The CTLA-4/G allele was found on 68.9% in type 1 patients as compared to 51.02% in controls (p = 0.002), mostly in homozygous from 43.24% versus 22.45% (p = 0.0058). This results indicate that CTLA-4/G allele was significantly associated with predisposition to type 1 diabetes in our group from Tunisian population.
authors.affiliation: Institut national de nutrition et de technologie alimentaire, 11, rue Jebal Lakhdhar Bab Saadoun 1006, Tunis, Tunisie.
authors.forename: H
authors.initials: H
authors.lastname: Kamoun Abid
authors.forename: S
authors.initials: S
authors.lastname: Hmida
authors.forename: N
authors.initials: N
authors.lastname: Smaoui
authors.forename: H
authors.initials: H
authors.lastname: Kaabi
authors.forename: A
authors.initials: A
authors.lastname: Abid
authors.forename: H
authors.initials: H
authors.lastname: Chaabouni
authors.forename: K
authors.initials: K
authors.lastname: Boukef
authors.forename: K
authors.initials: K
authors.lastname: Nagati
mesh_terms: D000069594:Abatacept; D000293:Adolescent; D000483:Alleles; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics*; D060908:CTLA-4 Antigen; D002648:Child; D002675:Child, Preschool; D002889:Chromosomes, Human, Pair 2; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D006801:Humans; D018796:Immunoconjugates; D007223:Infant; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011446:Prospective Studies; D014416:Tunisia
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D018796:Immunoconjugates; D000069594:Abatacept
vernacular_title: Etude de l'association entre diabète type 1 et polymorphisme du gène CTLA-4 dans une population tunisienne.