Interleukin-18 in patients with congestive heart failure: induction of atrial natriuretic peptide gene expression.
Activated inflammatory responses appear to play a role in the development of congestive heart failure (CHF). We investigated interleukin-18 (IL-18), which is a cytokine synthesized by activated macrophage, changes in patients with CHF. We evaluated 11 Japanese patients with angina pectoris (n=4) or CHF (n=7). Blood was sampled immediately after admission and at 1, 2, 3, 6, and 9 hours after admission and then every 12 hours until 5 days after admission. Plasma IL-18 concentrations were measured by an enzyme-linked immunosorbent assay. Expression of atrial natriuretic peptide (ANP) mRNA and protein synthesis was examined in cardiac myocyte by stimulation of IL-18. Plasma IL-18 concentration was significantly higher in patients with CHF than in 15 healthy volunteers (51+/-21 pg/mL, and 28+11 pg/mL, respectively, P<0.05). Increased expression of ANP mRNA was demonstrated in IL-18 treated myocytes. Protein synthesis in myocytes was increased by IL-18 in a dose-dependent manner. Increased secretion of IL-18 is induced in patients with CHF and correlates with the severity of myocardial damage and dysfunction.
Seta
Y
Y
Second Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Kanda
T
T
Tanaka
T
T
Arai
M
M
Sekiguchi
K
K
Yokoyama
T
T
Kurimoto
M
M
Tamura
J
J
Kurabayashi
M
M
D000328:Adult; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D015415:Biomarkers; D006332:Cardiomegaly / Q000097:blood; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D020382:Interleukin-18 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000096:biosynthesis; D009206:Myocardium / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats
D015415:Biomarkers; D020382:Interleukin-18; D009124:Muscle Proteins; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor