title: Pyrimidine base catabolism in Pseudomonas putida biotype B.
abstract: Reductive catabolism of the pyrimidine bases uracil and thymine was found to occur in Pseudomonas putida biotype B. The pyrimidine reductive catabolic pathway enzymes dihydropyrimidine dehydrogenase, dihydropyrimidinase and N-carbamoyl-beta-alanine amidohydrolase activities were detected in this pseudomonad. The initial reductive pathway enzyme dihydropyrimidine dehydrogenase utilized NADH or NADPH as its nicotinamide cofactor. The source of nitrogen in the culture medium influenced the reductive pathway enzyme activities and, in particular, dihydropyrimidinase activity was highly affected by nitrogen source. The reductive pathway enzyme activities in succinate-grown P. putida biotype B cells were induced when uracil served as the nitrogen source.
authors.affiliation: Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings 57007, USA. thomas_west@sdstate.edu
authors.forename: T P
authors.initials: TP
authors.lastname: West
mesh_terms: D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D016958:Pseudomonas putida / Q000145:classification* / Q000254:growth & development / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D013941:Thymine / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013386:Succinates; D009243:NAD; D009249:NADP; D014498:Uracil; D002244:Carbon; D005947:Glucose; D013941:Thymine