Brain-derived neurotrophic factor and vanilloid receptor subtype 1 immunoreactive sensory DRG neurons innervating the lumbar facet joints in rats.
The rat L5/6 facet joint is innervated from L1 to L6 by the dorsal root ganglia (DRG). The presence of substance P- and calcitonin gene-related peptide-immunoreactive (ir) DRG neurons innervating the L5/6 facet joint has been demonstrated. However, the presence of brain-derived neurotrophic factor (BDNF)-ir and the vanilloid receptor subtype 1 (VR1)-ir DRG neurons, which relate to inflammatory and burning pain innervating the L5/6 facet joint, has not. Fluoro-gold (FG)-labeled neurons innervating the L5/6 facet joint were distributed throughout the DRGs from T13 to L6 levels. Of the FG-labeled neurons, the proportions of BDNF-ir in L1, L2, L3, L4 and L5 DRG neurons were 9%, 15%, 21%, 17% and 20% and the proportions of VR1-ir L1, L2, L3, L4 and L5 DRG neurons were 8%, 9%, 15%, 16% and 15%, respectively.
Ohtori
S
S
Department of Orthopaedic Surgery, School of Medicine, Chiba University, Japan. s.c.oh@pref-hosp.togane.chiba.jp
Takahashi
K
K
Chiba
T
T
Yamagata
M
M
Sameda
H
H
Moriya
H
H
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D007596:Joints / Q000294:innervation*; D008159:Lumbar Vertebrae / Q000294:innervation*; D008297:Male; D008856:Microscopy, Fluorescence; D009475:Neurons, Afferent / Q000378:metabolism*; D009619:Nociceptors / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000378:metabolism*; D050916:TRPV Cation Channels; D016707:Tissue Fixation
D019208:Brain-Derived Neurotrophic Factor; D011955:Receptors, Drug; D050916:TRPV Cation Channels; C494484:TRPV1 receptor