title: Cytotoxicity of modern dentin adhesives--in vitro testing on gingival fibroblasts.
abstract: The present investigation was designed to test cellular toxicity of modern dentin adhesives. With the use of the products Ariston Liner, Etch & Prime 3.0, Optibond Solo, Prime & Bond NT, Scotchbond 1, and Syntac Sprint, test specimens were prepared according to the manufacturers' instructions and transferred into a culture medium. Eluates were obtained and pipetted onto fibroblast cultures, incubated, and subsequently stained. The respective cell densities and the numbers of normal, altered, and dead cells were determined and compared with control cell cultures. Statistical analysis of the data showed that all materials caused cytotoxic effects. Scotchbond 1 displayed the highest number of dead cells. The difference was statistically significant compared to Etch" 3.0, Optibond Solo, Prime&Bond NT, and the control. The lowest cell density was found for Scotchbond 1 and Ariston Liner. The difference was also statistically significant in comparison with Etch" 3.0, Optibond Solo, Prime&Bond NT, and the control. To conclude, all tested dentin adhesives caused cytotoxic reactions. Taking the limitations of an in vitro experiment into consideration, Prime&Bond NT, Optibond Solo, and Etch" 3.0 appear to be the most recommendable products, and Scotchbond 1 and Ariston Liner the least.
authors.affiliation: Department of Operative Dentistry, School of Dentistry, Johann Wolfgang Goethe University, Frankfurt am Main, Germany. S.Szep@em.uni-frankfurt.de
authors.forename: Susanne
authors.initials: S
authors.lastname: Szep
authors.forename: Astrid
authors.initials: A
authors.lastname: Kunkel
authors.forename: Karin
authors.initials: K
authors.lastname: Ronge
authors.forename: Detlef
authors.initials: D
authors.lastname: Heidemann
mesh_terms: D000179:Acrylates / Q000633:toxicity*; D000180:Acrylic Resins / Q000633:toxicity*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity*; D002452:Cell Count; D016923:Cell Death; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D011756:Diphosphates / Q000633:toxicity*; D000431:Ethanol / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D008298:Maleates / Q000633:toxicity*; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000633:toxicity*; D019279:Resin Cements / Q000633:toxicity*; D014617:Vacuoles / Q000187:drug effects
chemical_list: D000179:Acrylates; D000180:Acrylic Resins; D017220:Dentin-Bonding Agents; D011756:Diphosphates; C121187:Etch&Prime; D008298:Maleates; D008689:Methacrylates; C428805:OptiBond SOLO; D011109:Polymethacrylic Acids; C425843:Prime and Bond NT; D019279:Resin Cements; C443859:Syntac Sprint; C454691:ariston liner; D000431:Ethanol; D017438:Bisphenol A-Glycidyl Methacrylate; C041330:Scotchbond