title: Hepatocyte growth factor secreted by periodontal ligament and gingival fibroblasts is a major chemoattractant for gingival epithelial cells.
abstract: To ascertain whether periodontal fibroblasts could be involved in the pathogenesis of periodontal pocket formation, the chemotactic activity of periodontal ligament fibroblast-conditioned medium (PLF-CM) and gingival fibroblast-conditioned medium (GF-CM) for gingival epithelial cells was examined using a modified Boyden chamber assay. Both PLF-CM and GF-CM possessed significant chemotactic activity, which was decreased markedly by treatment with anti-human hepatocyte growth factor (HGF) neutralizing antibody. Furthermore, the chemotactic activity of PLF-CM and GF-CM was well correlated with HGF content. These results show that PLF and GF secrete an HGF-like factor, and suggest that such a factor derived from periodontal fibroblasts might play a role in epithelial apical migration in periodontitis.
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohshima
authors.forename: Y
authors.initials: Y
authors.lastname: Noguchi
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Maeno
authors.forename: K
authors.initials: K
authors.lastname: Otsuka
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002630:Chemotactic Factors / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D017228:Hepatocyte Growth Factor