Contribution of Hsc70 to barotolerance in the yeast Saccharomyces cerevisiae.
The contribution of Hsc70 to barotolerance in logarithmic-phase cells of the HSC70 (ssb1 and ssb2) deletion mutant and in strains expressing the HSC70 gene on either a low- or a high-copy-number plasmid was studied. The deletion-mutant strain had higher thermotolerance and a slightly lower barotolerance than the control strain. The strain that expresses the HSC70 gene in high copy number had a higher barotolerance than the strain that expresses the gene in low copy number. These results suggest that Hsc70 contributes to barotolerance during exponentially growing conditions as does Hsp104 during heat-shock treatment.
Iwahashi
H
H
National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan. hitoshi.iwahashi@aist.go.jp
Nwaka
S
S
Obuchi
K
K
D017353:Gene Deletion; D005800:Genes, Fungal; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006874:Hydrostatic Pressure; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014199:Trehalose / Q000378:metabolism
D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D014199:Trehalose