Expression characteristics of c-fos, c-myc and bFGF in early burn tissue.
OBJECTIVE
To investigate the sequence and characteristics of expression of proto-oncogenes, c-fos, c-myc and endogenous basic fibroblast growth factor (bFGF), in burn tissue from rats.


METHODS
A partial thickness burn of 30% TBSA was created on the backs of Wistar rats. Immunohistochemistry was used to detect the expression of these proteins in normal and burned skin at 3 hours, 6 hours, 1 day, 3 days, 7 days and 14 days postburn.


RESULTS
Expression of c-fos, c-myc and bFGF was induced by the burn, but these three proteins showed different expression levels in sequence and distribution. Expression of c-fos increased and peaked at 3 hours postburn. Stain positive granules were distributed mainly in the cytoplasm of basal cells and the nuclei of fibroblasts. The expression of bFGF protein increased at 6 hours and peaked at 1 day postburn; it was distributed in the cytoplasm of fibroblasts. c-myc protein peaked at 3 days postburn and was also distributed in the cytoplasm of fibroblasts.


CONCLUSIONS
Thermal injury can induce c-fos, c-myc and bFGF expression and show phasic control and regional distribution. The phasic expression of these three proteins indicates that there maybe a regulatory relationship between proto-oncogenes and growth factors in early burn. This may play an important role in late tissue repair.
Gu
X
X
Wound Healing Unit, 304th Hospital, Trauma Center of Postgraduate Medical College, Beijing 100037, China. Guxiaoman@21cn.net
Fu
X
X
Yang
Y
Y
Sun
T
T
Jiang
L
L
D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D016222:Fibroblast Growth Factor 2