Site-directed mutagenesis facilitated by DpnI selection on hemimethylated DNA.
Li
Fusheng
F
Department of Microbiology, University of Washington, Seattle, WA, USA.
Mullins
James I
JI
D019175:DNA Methylation; D017931:DNA Primers; D004277:DNA, Single-Stranded / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D007202:Indicators and Reagents; D008967:Molecular Biology / Q000379:methods; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D013698:Templates, Genetic
D017931:DNA Primers; D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific