[Human tongue carcinoma model established in nude mice by orthotopic transplantation and studies on its major biological properties].
OBJECTIVE
To establish clinically relevant animal model of human tongue carcinoma.


METHODS
Cell suspensions of human tongue squamous cell carcinoma cell line (Tca8113) were inoculated orthotopically into nude mice and the growing tumors were serially passaged by orthotopic transplantation. The oral cavity, mandible and neck of the nude mice were sectioned and examined histologically. The ultrastructure and chromosome of the tumors were also investigated.


RESULTS
1. The success rate of transplant was 90%. 2. The mouse-growing tumors exhibited the same identical properties as the donor tumor. 3. Human tongue cancer cells could grow in the homologous oral cavity site of nude mice, extended and metastasized to adjacent tissues and lymph node of the neck.


CONCLUSIONS
The model can be useful for evaluating biological behavior and new therapeutics for tongue cancer.
Yang
H
H
Hospital of Stomatology, Hubei Medical University, Wuhan 430070.
Li
J
J
Hu
C
C
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured