[Alterations of cell apoptosis and proliferation during transformation of a human bronchial epithelial cell line].
OBJECTIVE
To investigate the alterations of cell apoptosis and proliferation and its mechanism during transformation of a human bronchial epithelial cell line Y.


METHODS
Terminal deoxyribonucleotidyl transferase in situ labelling, BrdU incorporation, Western blotting and fluorescence in situ hybridization were used in the study.


RESULTS
During serial passages of the immortalized human bronchial epithelial cell line Y, some phenotypic alterations, such as higher colony forming efficiency in soft agar, resistance to serum-induced differentiation and low dependence on EGF were observed. The increase in cell proliferation and decrease in cisplatinum-induced apoptosis and the enhanced protein expression of Bcl-2, MDM2, PCNA and Cyclin D1 were found in the late passages of the cell line. The chromosomes of Y cells were diploid at the early passages, but became aneuploid in the late passages.


CONCLUSIONS
In addition to the increase in cell proliferation, inhibition of apoptosis might also contribute to the cytogenetic abnormality and the transformation of human bronchial epithelial cells.
Wang
Y
Y
Cancer Institute(Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Tong
T
T
Guo
S
S
D017209:Apoptosis; D001980:Bronchi / Q000473:pathology*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D019938:Cyclin D1 / Q000032:analysis; D004249:DNA Damage; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1