title: Cell-mediated immune responses (CMIR) to Rhinosporidium seeberi in mice.
abstract: There is no published data on Cell Mediated Immune Responses in experimental animals to Rhinosporidium seeberi the causative agent of human and animal rhinosporidiosis. The quantitative mouse foot-pad model was used to assay the Delayed-Type Hypersensitivity (DTH) cell-mediated immune response to extracts of purified endospores and sporangia of R. seeberi. Histological examination was used to confirm that the foot-pad reactions were compatible with DTH reactions in the mouse. We report that sonically disintegrated rhinosporidial endospores/sporangia induced DTH responses in the foot-pads of sensitized mice which were comparable in intensity and histological profile to that induced by sheep red blood cells in SRBC sensitized mice. Anti-rhinosporidial antibody was also induced. Filtrates of the soluble antigens in sonicated suspensions failed to evoke a DTH-foot-pad (DTH-FP) response in sensitized mice although an anti-rhinosporidial antibody response to this preparation was detected. Prolonged pre-treatment with sonicated suspensions of endospores and sporangia resulted in a decrease of DTH reactivity as compared with reactions following pre-treatment of a shorter duration.
authors.affiliation: Animal Centre, Medical Research Institute, Colombo, Sri Lanka.
authors.forename: S
authors.initials: S
authors.lastname: Jayasekera
authors.forename: S N
authors.initials: SN
authors.lastname: Arseculeratne
authors.forename: D N
authors.initials: DN
authors.lastname: Atapattu
authors.forename: R
authors.initials: R
authors.lastname: Kumarasiri
authors.forename: W M
authors.initials: WM
authors.lastname: Tilakaratne
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006651:Histocytochemistry; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000382:microbiology; D007111:Immunity, Cellular / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012227:Rhinosporidiosis / Q000276:immunology* / Q000473:pathology; D012228:Rhinosporidium / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D000907:Antibodies, Bacterial