[Effects of quinacrine on gut-origin bacteria/endotoxin translocation in rats with gut ischemia/reperfusion injury].
OBJECTIVE
To explore the influence of quinacrine, which is an inhibitor of phospholipase A2, on gut-origin bacteria/endotoxin translocation in rats with gut ischemia/reperfusion injury.


METHODS
Forty-two Wistar rats were randomly divided into normal control (C, n = 6), gut ischemia/reperfusion injury control (IC, n = 12), quinacrine use at 3 hours after the injury(3 h, n = 12), and quinacrine use at 12 hours after the injury (12 h, n = 12) groups. The contents of blood endotoxin and TNF alpha in venae cavae and portal vein were measured in all groups. At the same time, tissue samples from mesenteric lymph nodes, liver, lungs and kidneys were harvested for bacterial culture.


RESULTS
Plasma contents of endotoxin and TNF alpha were lowered in both quinacrine treatment groups (P < 0.01). The incidence of bacterial/endotoxin translocation was decreased by quinacrine use in mesenteric lymph nodes, lungs, liver and kidneys after the injury (P < 0.05 approximately 0.01).


CONCLUSIONS
Early application of quinacrine in Wistar rats inflicted by gut ischemia reperfusion injury could obviously lower bacterial/endotoxin translocation and ameliorate the release of pro-inflammatory mediators and cytokines, thus attenuating injury to remote organs.
Tu
W
W
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, P. R. China.
Chen
J
J
Xiao
G
G
D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects*; D004064:Digestive System / Q000098:blood supply / Q000187:drug effects* / Q000382:microbiology; D004731:Endotoxins / Q000097:blood / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008297:Male; D008643:Mesentery; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004731:Endotoxins; D014409:Tumor Necrosis Factor-alpha; D011796:Quinacrine