Expression of interleukin-1beta mRNA in murine uterine and gestational tissues: relationship with gestational age.
OBJECTIVE
The pro-inflammatory cytokine interleukin (IL)-1beta has been shown to stimulate the production of prostaglandins (PG) in gestational tissues. Increased PG synthesis is considered a key step in the initiation of labor both at term and preterm. In this study. IL-1beta mRNA in the uterus and gestational tissues of mice during mid to late pregnancy was studied to characterize its tissue specific as well as gestational age expression.


METHODS
Gestational tissues (placenta. decidual cap and fetal membranes). uterus, and cervix were collected from pregnant mice during gestation. Total RNA was isolated and probed for the expression of IL-1beta mRNA.


RESULTS
There was a significantly increased expression of IL-1beta mRNA in the uterus on day 18 of pregnancy. In the decidual caps, there was increased expression of IL-1beta mRNA on day 14 of pregnancy and a decrease in expression with the onset of labor. In the fetal membranes and placenta, IL-1beta mRNA expression significantly increased on days 14 and 18 of pregnancy. respectively, and then remained elevated for the duration of pregnancy. In the cervix, there was a decrease in expression with labor onset.


CONCLUSIONS
The increases in IL-1beta mRNA in the fetal membranes and placenta late in pregnancy are consistent with a localized, tissue specific inflammatory activation involved in the initiation of parturition.
Sato
T A
TA
Liggins Institute and Division of Pharmacology and Clinical Pharmacology, Faculty of Medical and Health Sciences, University of Auckland, New Zealand. t.sato@auckland.ac.nz
Gupta
D K
DK
Keelan
J A
JA
Marvin
K W
KW
Mitchell
M D
MD
D000818:Animals; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D011270:Pregnancy, Animal; D012333:RNA, Messenger; D014599:Uterus / Q000276:immunology*
D007375:Interleukin-1; D012333:RNA, Messenger