[FHIT gene is abnormal in laryngeal carcinoma].
OBJECTIVE
To determine the role of FHIT gene in laryngeal carcinogenesis.


METHODS
Laryngeal cancer cell line Hep-2, HeLa cell line and 80 primary tumors from patients with laryngeal cancer were studied by means of RT-PCR, sequencing and microsatellite analysis.


RESULTS
Abnormal transcripts of FHIT gene were found in the two cell lines and 70% (14/20) of primary laryngeal carcinomas. Aberrant laryngeal tumor transcripts often lack more than two exons of FHIT gene. Microsatellite analysis showed that both loss of heterozygosity and microsatellite instability of FHIT gene existed in laryngeal carcinoma and the former was more common.


CONCLUSIONS
FHIT gene participates in the laryngeal carcinogenesis and may be one of the candidate tumor suppressor genes.
Fu
W
W
Department of Medical Genetics, China Medical University, Shenyang 110001.
Wu
D
D
Zhang
X
X
D017766:Acid Anhydride Hydrolases; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009363:Neoplasm Proteins / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase