Oligoclonal T-cells in blood and target tissues of patients with anti-Hu syndrome.
T-cell clones of unknown significance (TCUS), assessed by monoclonal or oligoclonal T-cell patterns in PCR-DGGE, were detected in blood of 7/9 patients with anti-Hu syndrome. Clonal patterns were also detected in 2/2 neoplastic lymph nodes, and in 2/2 inflamed dorsal root ganglia from three patients. Only some T-cell clones found in target tissues were also detected in blood or non-target tissues, and likely corresponded to TCUS. In one patient, an identical T-cell clone was found in both neoplastic lymph node tissue and dorsal root ganglia, but not in blood. Dorsal root-infiltrating lymphocytes were cytotoxic CD8(+) TIA-1(+) T-cells. They were often found in close contact to sensory neurons, most of which expressed MHC-1. Taken together, these data support a direct effector role of cytotoxic CD8(+) T-cells, the same clones being likely operative in sensory neuron damage and immune-mediated tumor growth control.
Plonquet
A
A
INSERM E0011, "Système neuromusculaire et inflammation", Faculté de Médecine Paris XII, 94010, Créteil, France.
Gherardi
R K
RK
Créange
A
A
Antoine
J C
JC
Benyahia
B
B
Grisold
W
W
Drlicek
M
M
Dreyfus
P
P
Honnorat
J
J
Khouatra
C
C
Rouard
H
H
Authier
F J
FJ
Farcet
J P
JP
Delattre
J Y
JY
Delfau-Larue
M H
MH
D000328:Adult; D000368:Aged; D002999:Clone Cells; D051959:ELAV Proteins; D005260:Female; D005727:Ganglia, Spinal / Q000473:pathology; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009443:Neuritis / Q000276:immunology; D020364:Paraneoplastic Polyneuropathy / Q000276:immunology* / Q000473:pathology; D016601:RNA-Binding Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology*
D051959:ELAV Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins