title: Combined use of LC/MS and a biological test for rapid identification of marine mycotoxins produced by Trichoderma koningii.
abstract: Trichoderma koningii Oudemans, a strain isolated from a shellfish farming area, was selected for its high frequency in samples and its ability to produce metabolites when cultured in natural seawater. Combined use of LC/MS and a biological test on blowfly larvae allowed the characterization of four compounds after purification in only two steps (VLC and HPLC). ESI/MS, a powerful tool for rapid identification and sequence determination of peptides, confirmed that these compounds were peptide, alpha-aminoisobutyric acid and amino alcohol (peptaibols), the usual metabolites of Trichoderma.
authors.affiliation: ISOMer, SMAB-URM11 (Toxinologie marine), Faculté de Pharmacie, Université de Nantes, BP 53508, 44035 Nantes Cedex 01, France. Anne.Landreau@sante.univ-nantes.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Landreau
authors.forename: Yves François
authors.initials: YF
authors.lastname: Pouchus
authors.forename: Claire
authors.initials: C
authors.lastname: Sallenave-Namont
authors.forename: Jean François
authors.initials: JF
authors.lastname: Biard
authors.forename: Marie Claude
authors.initials: MC
authors.lastname: Boumard
authors.forename: Thibaut
authors.initials: T
authors.lastname: Robiou du Pont
authors.forename: Florence
authors.initials: F
authors.lastname: Mondeguer
authors.forename: Christophe
authors.initials: C
authors.lastname: Goulard
authors.forename: Jean François
authors.initials: JF
authors.lastname: Verbist
mesh_terms: D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis*; D014242:Trichoderma / Q000737:chemistry* / Q000472:pathogenicity
chemical_list: D009183:Mycotoxins; D010455:Peptides