Serum granulocyte colony-stimulating factor in patients with chronic renal failure.
OBJECTIVE
To gain a better understanding of the regulatory mechanism and kinetic behaviour of granulocyte colony-stimulating factor (G-CSF).


METHODS
An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF in 61 patients with chronic renal failure +/- long-term hemodialysis and 30 normal controls.


RESULTS
Serum G-CSF levels in CRF patients were significantly higher than in normal controls. Eighty percent of patients had detectable G-CSF and serum G-CSF levels were 566.40 +/- 207.98 ng/L in non-hemodialyzed (non-HD) patients. The detectable percentage in hemodialyzed patients was 93.33%, serum G-CSF levels in pre-HD and post-HD patients were 1255.36 +/- 611.25 ng/L and 1151.61 +/- 599.47 ng/L respectively. Serum G-CSF levels in HD patients were slightly higher than in non-HD patients, but no significant difference was found between the two groups. No difference was found between the G-CSF values obtained in pre-HD and post-HD patients. There was no relationship between G-CSF levels and WBC, BUN or Scr (P > 0.05).


CONCLUSIONS
The high value of G-CSF in patients with CRF may be caused by a decrease in G-CSF clearance and/or an increase in G-CSF release.
Wu
W
W
Laboratory of Cellular Biology, Shanghai Institute of Hematology, Shanghai Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Sun
G
G
Tian
G
G
Wang
Z
Z
D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008297:Male; D008875:Middle Aged
D016179:Granulocyte Colony-Stimulating Factor