Molecular detection of Campylobacter spp. in drinking, recreational and environmental water supplies.
A molecular detection assay was performed on 207 samples of drinking, recreational and environmental waters collected in Northern Ireland. The water sources which were PCR positive for Campylobacter spp. included 2/91 (2.2%) drinking water from domestic household taps, 5/57 (8.8%) swimming pool water, 1/23 (4.3%) lake water and 1/1 water from a jacuzzi. Extracted DNA from all water samples was amplified employing a sequence-specific PCR assay based on a 206 bp conserved region of the flagellin A-flagellin B (flaA/flaB) loci for Campylobacter jejuni, C. coli and C. lari. Given the physiological and cultural fragile nature of these species, no waters were cultured using conventional methods due to concern for reversion to non-culturability from time of collection to laboratory analysis. As this genus has been demonstrated to form a 'viable but non-culturable' (VBNC) form, failure to culture organisms conventionally from water does not necessarily equate to a negative result, hence molecular detection assays, especially those which can demonstrate cell viability, may be useful in helping to elucidate potential epidemiological sources and reservoirs of this organism, especially where water is suspected as being the vehicle of transmission.
Moore
J
J
Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK. jemoore@niphl.dnet.co.uk
Caldwell
P
P
Millar
B
B
D000900:Anti-Bacterial Agents / Q000032:analysis; D016227:Benzoquinones / Q000032:analysis; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D016021:Epidemiologic Studies; D005188:False Negative Reactions; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013551:Swimming Pools; D014871:Water Microbiology; D014881:Water Supply
D000900:Anti-Bacterial Agents; D016227:Benzoquinones; D004269:DNA, Bacterial; C403874:flagranone A; C403875:flagranone B