Microencapsulation of carbon particles used as oxygen sensors in EPR oximetry to stabilize their responsiveness to oxygen in vitro and in vivo.
The electron paramagnetic resonance (EPR) spectra of some paramagnetic materials exhibit a pO2 (partial pressure of oxygen)-dependent linewidth. By recording the EPR linewidth in vivo using low-frequency EPR spectrometers, it is possible to measure the partial pressure of oxygen in tissues. It has been found, however, that some of the paramagnetic materials with optimal spectroscopic properties in vitro may lose or change their responsiveness to oxygen in tissues. The aim of this study was to microencapsulate paramagnetic particles by biopolymers in order to stabilize their responsiveness to oxygen. Carbohydrate char particles (Bubinga) were encapsulated with different biopolymers: cellulose acetate or cellulose triacetate, silicone and polyurethane. The performance of the materials was evaluated in vitro and in vivo. X-band EPR spectroscopy was used to test the variation of the calibration curve (EPR linewidth as a function of the pO2) after incubation in saline and after prolonged residence in tissues. The stability of the responsiveness to PO2 in vivo was carried out by L-band EPR spectroscopy using mice that received injection of the oxygen sensors in the muscles. After residence in saline and prolonged residence in tissues, only the calibration curve of the silicone-coated (coating weight of 0.5% (w/w)) paramagnetic materials remained unchanged, while those of oxygen sensors coated with cellulose acetate, cellulose triacetate and polyurethane changed.
Jiang
H
H
Laboratory of Medicinal Chemistry and Radiopharmacy, Université catholique de Louvain, Brussels, Belgium.
Beghei
N
N
Clarkson
R B
RB
Swartz
H M
HM
Galle
B
B
D000818:Animals; D002138:Calibration; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008863:Microspheres; D010100:Oxygen / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D013997:Time Factors
D002241:Carbohydrates; D011108:Polymers; D002244:Carbon; D010100:Oxygen