[A quick way in isolation and amplification of mandibular condylar cartilage cell in vitro].
OBJECTIVE
To establish a quick way in acquiring well differentiated mandibular condylar cartilage (MCC) cells with high viability in large scale.


METHODS
Japan white rabbit MCC cells were harvested by enzymatic method. They were grown in a modified bioreactor culture system, which contained the cytodex-3 micro-carriers in the culture medium. Kinetic growth of MCC cells on DEAE-dextran micro-carrier was observed under phase contrast microscope and environmental scanning microscope respectively.


RESULTS
MCC cells attached rapidly to the surface of micro-carriers, but their spreading was slow. A quick growth of these cells was observed when they fully spread onto the micro-carrier. The number of MCC cells increased 16.2 times compared with that of plating.


CONCLUSIONS
Micro-carrier culture of MCC cells can yield a large quantity of cells within a short period of time that will be of benefit in banking MCC cells for reconstruction of impaired cartilage.
Jiao
Y
Y
Department of Radiology, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Ma
X
X
Yu
S
S
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000494:pharmacology; D008335:Mandibular Condyle / Q000166:cytology*; D011817:Rabbits; D013997:Time Factors
D003911:Dextrans; C052839:Cytodex