New solvent-producing Clostridium sp. strains, hydrolyzing a wide range of polysaccharides, are closely related to Clostridium butyricum.
Thirteen new Clostridium strains, previously isolated from soil and found to produce high amounts of solvents from glucose, hydrolyzed a great variety of alpha- and beta-glycans, including raw starch, xylan, pectin, inulin and cellulose. The sequences of the PCR-amplified DNA fragments containing the variable 3' part of one of the 16S rRNA genes were 99.5% identical. The macrorestriction pattern of two endonucleolytic digests of chromosomal DNA in the pulsed-field gel electrophoresis (PFGE) confirmed their high homogeneity on the DNA level. The complete 16S rRNA gene sequence of three selected strains was 99.8% identical to the 16S rRNA gene sequence from Clostridium butyricum and separates them from C. acetobutylicum. To the closely related four species of solventogenic clostridia a new group of strains has to be added, which has a great potential for the direct fermentation of biomass.
Montoya
D
D
Institute of Biotechnology, Universidad Nacional de Colombia, AA 14490, Santafé de Bogotá, Colombia.
Arévalo
C
C
Gonzales
S
S
Aristizabal
F
F
Schwarz
W H
WH
D018533:Biomass; D003013:Clostridium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D012997:Solvents / Q000378:metabolism*
D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; D012997:Solvents