Serological cloning of PARIS-1: a new TBC domain-containing, immunogenic tumor antigen from a prostate cancer cell line.
Identifying immunogenic tumor antigens plays a critical role in developing efficient diagnostic and therapeutic strategies for treatment of cancer. Using a recently developed technology, serological identification of antigens by recombinant expression cloning (SEREX), we identified a total of 8 genes whose expression elicited antibody responses in prostate cancer patients. Of the 8 genes, 5 represented known genes in the GenBank database, 2 were previously uncharacterized genes, and 1 showed sequence homology to a mouse gene. The sequence feature and the expression of one of the novel genes, prostate antigen recognized and identified by SEREX (PARIS-1), are determined in this study. The PARIS-1 cDNA is 3257 bp in length and contains a complete open reading frame of 2751 bp encoding for a primary translation product of 917 amino acids. Using Northern blot hybridization assay, we detected a single species of approximately 3.3 kb PARIS-1 mRNA that is differentially expressed in prostate normal and cancer cells. Western blot analysis confirmed the expression of the PARIS-1 protein in these cells. Structure analysis revealed that PARIS-1 protein contains a TBC domain that is conserved in the family of cell cycle-regulatory and Rab GTPase-activating proteins (Rab-GAP). Thus, the PARIS-1 protein may play a role in regulation of cell differentiation and growth or represent a new member of the Rab-GAP family.
Zhou
Yaling
Y
Department of Molecular Medicine, Northwest Hospital, Suite 101, Bothell, Washington 98021, USA. yaling@nwbio.com
Toth
Monica
M
Hamman
M Shane
MS
Monahan
Steven J
SJ
Lodge
Patricia A
PA
Boynton
Alton L
AL
Salgaller
Michael L
ML
D000595:Amino Acid Sequence; D000906:Antibodies / Q000097:blood; D000918:Antibody Specificity / Q000276:immunology; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods; D017124:Conserved Sequence / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D011467:Prostate / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012698:Serologic Tests / Q000379:methods; D020691:rab GTP-Binding Proteins / Q000235:genetics
D000906:Antibodies; D000951:Antigens, Neoplasm; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; C446300:PARIS-1 antigen; D012333:RNA, Messenger; D020691:rab GTP-Binding Proteins