Modulation of experimental allergic encephalomyelitis in Lewis rats by administration of a peptide of Fas ligand.
The effects of modulation of apoptosis in experimental allergic encephalomyelitis (EAE) in Lewis rats have been investigated using a peptide of the Fas-Ligand protein (FasL-p). The peptide was administered both subcutaneously and intra-cerebro-ventricularly (i.c.v.) after EAE induction. Rats treated subcutaneously with FasL-p showed a worse clinical score as compared to saline treated animals, while i.c.v. treatment with FasL-p did not modify significantly the severity of EAE. Apoptotic lymphomonocytes (identified by TUNEL) infiltrating the brain and the spinal cord were decreased in rats treated i.c.v. with FasL-p. The data suggest that the Fas/Fas-ligand pathway may be modulated by treatments with peptides of Fas-Ligand and that it may be at work within the central nervous system in EAE.
Ciusani
E
E
Istituto Nazionale Neurologico 'C. Besta', Via Celoria 11, Milan, 20133, Italy.
Gelati
M
M
Frigerio
S
S
Pollo
B
B
Massa
G
G
Sacerdote
P
P
Panerai
A E
AE
Salmaggi
A
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D053222:Fas Ligand Protein; D006801:Humans; D019169:Jurkat Cells; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D019014:fas Receptor / Q000276:immunology
C505661:FASLG protein, human; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D010455:Peptides; D019014:fas Receptor