In situ analysis of the transcriptional activity of integrated viral DNA using tyramide-FISH.
Infection by the oncogenic human papillomavirus (HPV) types 16 and type 18 can progress to cancers. Two well studied cervical carcinoma cell lines, SiHa and CaSki, contain two to four copies, or several hundred copies of integrated HPV-16, respectively. To define the chromosomal loci from which HPV mRNAs are transcribed in these cells, we have simultaneously visualized chromosomal DNA territories, HPV DNA or nascent HPV RNA sequences by using a highly sensitive in situ hybridization (T-FISH) technique employing deposition of fluorescent tyramides. We found that, in SiHa cells, nascent HPV RNAs co-localized with both integrated HPV copies on chromosome 13. Surprisingly, in CaSki cells, nascent HPV RNA only co-localized with one minor HPV DNA-positive locus on chromosome 14. The DNA signal intensity of this locus was consistent with a single to a few HPV intergrants. The tyramide methodologies described here provide an in-depth molecular cytological analyses applicable to research and diagnosis.
Van Tine
B A
BA
Department of Molecular and Cellular Pathology, University of Alabama at Birmingham, 35294, USA.
Knops
J
J
Broker
T R
TR
Chow
L T
LT
Moen
P T
PT
D000577:Amides / Q000737:chemistry*; D004279:DNA, Viral / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic
D000577:Amides; D004279:DNA, Viral; D012367:RNA, Viral