Detection of human herpesvirus 8 DNA in acute leukemia patients.
OBJECTIVE
To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemia (AL) patients.


METHODS
The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined using polymerase chain reaction (PCR). Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included.


RESULTS
HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AML). The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive. None of the normal donors and human hematopoietic cell lines showed evidence of HHV-8 DNA.


CONCLUSIONS
The results suggest that the prevalence of HHV-8 is low in AL in China.
Wang
M
M
Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
Song
Y
Y
Ma
X
X
Han
M
M
Bi
Y
Y
Mu
G
G
Lin
Y
Y
Li
G
G
Wu
K
K
D000208:Acute Disease; D004279:DNA, Viral / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D007938:Leukemia / Q000821:virology*
D004279:DNA, Viral