FGF-2 increases colony formation, PTH receptor, and IGF-1 mRNA in mouse marrow stromal cells.
FGF-2 stimulates bone formation in vitro and in vivo in rats. However, there are limited studies in mice and no data on the mechanism(s) by which FGF-2 induces bone formation. We assessed whether short-term FGF-2 treatment of marrow stromal cells from young mice would increase alkaline phosphatase-positive (ALP), mineralized colony formation and expression of genes important in osteoblast maturation. Short-term treatment with FGF-2 (0.01-1.0 nM) for the first 3 days of a 14- or 21-day culture period increased the number of ALP mineralized colonies in bone marrow stromal cells. FGF-2 (0.1 nM) increased the mRNAs for type 1 collagen: osteocalcin, runt domain/core binding factor, PTH/PTHR receptor, and insulin-like growth factor 1 (IGF-1) at 14 and 21 days. We conclude that short-term FGF-2 treatment enhances osteoblast maturation in vitro. Furthermore, the anabolic effect of FGF-2 may be attributed in part to regulation of IGF-1 in osteoblasts.
Zhang
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University of Connecticut School of Medicine, Farmington, CT 06030, USA.
Sobue
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Hurley
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D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D001842:Bone and Bones / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins; D015675:Osteocalcin / Q000096:biosynthesis; D010281:Parathyroid Hormone / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017154:Stromal Cells / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism
D024042:Collagen Type I; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D010281:Parathyroid Hormone; D012333:RNA, Messenger; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I