Monoclonal antibodies to lymphocyte surface antigens for cetacean homologues to CD2, CD19 and CD21.
CD2 is a pan-T cell marker, while CD19 and CD21 are important molecules in signal transduction of B lymphocytes. CD19 and CD21 are both present on mature B cells, while CD19 is also present in developing B cells and plasma cells. Monoclonal antibodies (mAbs) against cetacean lymphocyte putative homologues to CD2 (two different antibodies), CD19 and CD21 were characterized. The proteins immunoprecipitated were as follows: F21.I (putative anti-CD2), 43 and 59kDa; F21.B (putative anti-CD19), 83 and 127kDa; F21.F (putative anti-CD21), 144kDa. The second putative anti-CD2 (F21.C) selectively inhibited the binding of F21.I. Both the putative anti-CD2 (T cell markers) stained T-cell zones on lymph node sections, while both the B cell markers (putative CD19 and CD21) stained B-cell zones. F21.B and F21.F were absent from thymus single cell suspension but labeled 63 and 65% mesenteric lymph node lymphocytes, respectively, while both F21.C and F21.F were present on 100% thymocytes and fewer lymph node lymphocytes. B and T cell markers were mutually exclusive on double labeling using flow cytometry. These mAbs are foreseen as possible valuable diagnostic and research tools to assess immune functions of captive and wild cetaceans.
De Guise
Sylvain
S
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. sdeguise@canr.uconn.edu
Erickson
Karen
K
Blanchard
Myra
M
DiMolfetto
Lisa
L
Lepper
Heather D
HD
Wang
Janice
J
Stott
Jeffrey L
JL
Ferrick
David A
DA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D018941:Antigens, CD19 / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D018801:CD2 Antigens / Q000276:immunology; D002591:Cetacea / Q000276:immunology*; D004289:Dolphins / Q000276:immunology; D005434:Flow Cytometry; D016131:Lymphocyte Subsets / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D011233:Precipitin Tests; D017464:Receptors, Complement 3d / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D018941:Antigens, CD19; D000954:Antigens, Surface; D018801:CD2 Antigens; D017464:Receptors, Complement 3d