[Clinical study on the expression level of lymphocyte function-related antigen of breast cancer patients].
OBJECTIVE
To explore the protein expression level of the lymphocyte function-related antigen CD11a, CD18, CD54 of breast cancer patients' blood, cancer tissue and drainage lymph node, and the effect of chemotherapy as well as Yiqi Yangxue Granule on its expression level.


METHODS
The immunohistochemical assay and flow cytometry were used to determine the change of the protein expression level of CD11a, CD18, CD54 on the lymphocyte of blood, cancer tissue and lymph node between before and after chemotherapy and chemotherapy plus Yiqi Yangxue Granule in 72 patients suffering from breast cancer.


RESULTS
In breast cancer patients, the level of CD54 was increased more obviously than that of control group, the CD11a, CD18, CD54 of lymphocytes in cancer tissue increased remarkably, while the CD54 expression positive rate reached 100%, the expression level of CD11a, CD18, CD54 which increased significantly in negative lymph node obviously higher than that of positive lymph node.


CONCLUSIONS
The level of adherence cell was markedly abnormal in breast cancer patients, whose immune adherence function disturbed. Although chemotherapy kills the tumor cells, it damages the immune adherence function, Yiqi Yangxue Granule could improve the immune function and enhance the anti-cancer effect of patients.
Zhang
J
J
Affiliated Tumor Hospital of Tianjin Medical University, Tianjin (300060).
Wu
X
X
Huang
J
J
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D003520:Cyclophosphamide / Q000008:administration & dosage; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000097:blood; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage; D008875:Middle Aged; D009361:Neoplasm Invasiveness
D004365:Drugs, Chinese Herbal; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D003520:Cyclophosphamide; D005472:Fluorouracil; D008727:Methotrexate