Laser desorption mass spectrometry for microbial DNA analysis.
Recently, we demonstrated that a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) can be used to determine the molecular weight of polymerase chain reaction (PCR) products of intact 16S rRNA regions and to profile their restriction digests. This is the first time that MALDI-TOF MS with ultraviolet (UV) photoionization has been used to analyze a PCR product of approximately 1600 nucleotides in length.
Taranenko
N I
NI
Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831-6378, USA.
Hurt
R
R
Zhou
J Z
JZ
Isola
N R
NR
Huang
H
H
Lee
S H
SH
Chen
C H
CH
D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S