Expression, specificity and immunotherapy potential of prostate-associated genes in murine cell lines.
The TRAMP-C1 (C1) and TRAMP-C2 (C2) cell lines were derived from a prostate tumor that arose in a mouse from the transgenic adenocarcinoma mouse prostate (TRAMP) model. However, their similarity to primary prostate tumors and therefore their usefulness in immunotherapy studies has not been clearly defined. We showed using RT-PCR that these cell lines exhibited a variety of prostate-specific genes expressed by human prostate tumors that may be used as tumor-associated antigens for immunotherapy. Interestingly, several of these genes are also expressed in cell lines that are not prostatic in origin. The prostate cell lines were also shown to grow in an androgen-independent manner, to be capable of expressing MHC class I and to be susceptible to specific lysis by cytotoxic T lymphocytes. Therefore, these cell lines will provide us with the ability to evaluate immune responses to and tolerance of prostate-specific protein peptides in an animal model.
Grossmann
M E
ME
Department of Urology, Mayo Clinic, Rochester, MN 55905, USA. grossmann.michael@mayo.edu
Wood
M
M
Celis
E
E
D000135:Acid Phosphatase; D000230:Adenocarcinoma / Q000235:genetics* / Q000276:immunology / Q000628:therapy*; D000818:Animals; D000918:Antibody Specificity / Q000235:genetics* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D002268:Carboxypeptidases / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins; D015870:Gene Expression / Q000235:genetics* / Q000276:immunology; D016147:Genes, Tumor Suppressor / Q000502:physiology; D043425:Glutamate Carboxypeptidase II; D018398:Homeodomain Proteins / Q000235:genetics / Q000276:immunology; D007167:Immunotherapy; D066298:In Vitro Techniques; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000276:immunology / Q000628:therapy*; D029584:Prostatic Secretory Proteins / Q000235:genetics / Q000276:immunology; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000502:physiology*
D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; C484414:HOXB13 protein, human; D018398:Homeodomain Proteins; C100943:Hoxb13 protein, mouse; D008562:Membrane Glycoproteins; C491178:NKX3-1 protein, human; D009363:Neoplasm Proteins; C102837:Nkx3-1 protein, mouse; D029584:Prostatic Secretory Proteins; C504399:Psca protein, mouse; D014157:Transcription Factors; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases; D002268:Carboxypeptidases; D043425:Glutamate Carboxypeptidase II