[Study on effect and mechanism of essential garlic oil in inhibiting monocyte-vascular endothelial cell adhesion induced by interleukin-1 alpha].
OBJECTIVE
To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule-1 (VCAM-1) expression of endothelial cell and monocyte adhesion induced by interleukin-1 alpha (IL-1 alpha).


METHODS
Human umbilical vein endothelial cells (HUVEC) were isolated by pancreatin digestion method and co-cultured with U937 monocyte and IL-1 alpha or EGO + IL-1 alpha respectively. The adhesion rate of monocyte to endothelial cell was measured and mean fluorescent intensity of immuno-stained of VCAM-1 was determined by ACAS 570.


RESULTS
EGO showed significant inhibition on VCAM-1 expression induced by IL-1 alpha either in simple culture with HUVEC or in co-culture with HUVEC + U937, and could obviously down regulate the monocyte-endothelial cell adhesion under effect of IL-1 alpha.


CONCLUSIONS
EGO has the effect of antagonizing against adhesion of monocyte and vascular endothelial cell, it may be due to the inhibition of EGO on adhesive molecular expression on endothelial cell.
Ge
L
L
First Military Medical University, Guangzhou (510515).
Zhang
W
W
Dai
Y
Y
D000498:Allyl Compounds / Q000494:pharmacology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D013440:Sulfides / Q000494:pharmacology*; D020298:U937 Cells / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D000498:Allyl Compounds; D007375:Interleukin-1; D013440:Sulfides; D019010:Vascular Cell Adhesion Molecule-1; C038491:allyl sulfide