title: Two-dimensional gel electrophoresis of selenized yeast and autoradiography of 75Se-containing proteins.
abstract: Two-dimensional high-resolution gel electrophoresis (2DE) has been applied to the fractionation of 75Se-containing proteins in yeast, grown in 75Se-containing medium, and autoradiography was used for detection of the 75Se-containing proteins. Gel filtration and ultrafiltration were used to check whether the selenium side-chains were stable in the presence of the chemicals used for lysis and 2DE. The mass distribution of the selenium-containing proteins was estimated by use of gel filtration and the results were compared with the distribution obtained by 2DE. A 2DE map of selenium-containing proteins in yeast is presented, and compared with a total protein map of yeast.
authors.affiliation: Laboratory of Analytical Chemistry, Ghent University, Belgium. cyrille.chery@rug.ac.be
authors.forename: C C
authors.initials: CC
authors.lastname: Chéry
authors.forename: E
authors.initials: E
authors.lastname: Dumont
authors.forename: R
authors.initials: R
authors.lastname: Cornelis
authors.forename: L
authors.initials: L
authors.lastname: Moens
mesh_terms: D001345:Autoradiography; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D017279:Selenocysteine / Q000737:chemistry; D014462:Ultrafiltration
chemical_list: D005656:Fungal Proteins; D016566:Organoselenium Compounds; D017279:Selenocysteine