title: Food deprivation-induced expression of minoxidil sulfotransferase in the hypothalamus uncovered by microarray analysis.
abstract: We used oligonucleotide microarrays to analyze comprehensively hypothalamic gene expression changes that correlate with energy homeostasis. We compared the hypothalamic gene expression profiles of freely fed and 48-h fasted rats using 26,379 oligonucleotide probe sets. Expression of 96 genes was up-regulated and expression of 73 genes was down-regulated in a statistically significant manner with fasting. The gene encoding the enzyme minoxidil sulfotransferase, an enzyme that catalyzes the transfer of sulfonate groups to biogenic amines and other substrates, was foremost among a set of genes whose mRNAs were uniformly detectable and displaying the greatest transcriptional changes with fasting. Northern blot analysis indicated that minoxidil sulfotransferase mRNA is up-regulated in the fasted rat and mouse, ob/ob mouse, and fa/fa rat. Results of reverse transcription quantitative PCR indicated that minoxidil sulfotransferase mRNA is also up-regulated in the microdissected arcuate and paraventricular nuclei of the fasted rat. Several index genes known to be either up-regulated (neuropeptide Y) or down-regulated (amphetamine-regulated transcript and proopiomelanocortin) with fasting were also found to be present among our set of "differentially expressed" genes. This study identifies a novel gene induced by fasting and demonstrates the feasibility of using oligonucleotide microarrays for the study of complex neuronal processes.
authors.affiliation: Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: Ji-Yao
authors.initials: JY
authors.lastname: Li
authors.forename: Pascal A
authors.initials: PA
authors.lastname: Lescure
authors.forename: David E
authors.initials: DE
authors.lastname: Misek
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Lai
authors.forename: Biao-Xin
authors.initials: BX
authors.lastname: Chai
authors.forename: Rork
authors.initials: R
authors.lastname: Kuick
authors.forename: Robert C
authors.initials: RC
authors.lastname: Thompson
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Demo
authors.forename: David M
authors.initials: DM
authors.lastname: Kurnit
authors.forename: George
authors.initials: G
authors.lastname: Michailidis
authors.forename: Samir M
authors.initials: SM
authors.lastname: Hanash
authors.forename: Ira
authors.initials: I
authors.lastname: Gantz
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005508:Food Deprivation; D007031:Hypothalamus / Q000201:enzymology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015238:Sulfotransferases / Q000235:genetics*
chemical_list: D015238:Sulfotransferases; C066827:minoxidil sulfotransferase