Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD.
We have developed a route for the synthesis of 2'-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.
Du
Quan
Q
Department of Chemistry, Brooklyn College, The City University of New York, 2900 Bedford Avenue, Brooklyn, New York 11210, USA.
Carrasco
Nicolas
N
Teplova
Marianna
M
Wilds
Christopher J
CJ
Egli
Martin
M
Huang
Zhen
Z
D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D009841:Oligonucleotides; D016566:Organoselenium Compounds; D012313:RNA; D004247:DNA