[Genistein exerts multiple suppressive effects on human breast carcinoma cells].
OBJECTIVE
To study the suppressive effects of genistein on progression of breast carcinoma.


METHODS
In vitro cell culture, animal studies, zymography, Northern and Western blot were used in this study.


RESULTS
Genistein inhibited in vitro invasion of MCF-7 and MDA-MB-231 cells. This inhibition was associated with down-regulation of MMP-9 and up-regulation of TIMP-1. These effects were also demonstrated in vivo. Genistein inhibited growth of the breast cancer cells transplanted in nude mice. The growth inhibitory effect was associated with increased tumor cell apoptosis, and up-regulated p21WAF1/CIP1 expression. Furthermore, genistein inhibited angiogenesis as shown by decreased vessel density and decreased production and release of VEGF and TGF-beta 1.


CONCLUSIONS
Genistein exerts multiple suppressive effects on breast carcinoma. It may help halt its progression.
Shao
Z
Z
Department of Surgery, Molecular Biology Laboratory, Cancer Hospital/Cancer Institute, Shanghai Medical University, Shanghai 200032, China.
Wu
J
J
Shen
Z
Z
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D019833:Genistein / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000517:prevention & control*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D019715:Tissue Inhibitor of Metalloproteinase-1; D019833:Genistein; D020780:Matrix Metalloproteinase 9