High-performance liquid chromatographic procedure for routine residue monitoring of seven sulfonamides in milk.
A simplified method for routine monitoring of 7 residual sulfonamides (SAs) (sulfadiazine (SDZ), sulfamerazine (SMR), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfadimethoxine (SDM), and sulfaquinoxaline (SQ)) in milk using high-performance liquid chromatography (HPLC) with a photodiode array detector is described. The spiked and blank samples were cleaned up by using an Ultrafree-MC/PL centrifugal ultrafiltration unit. For determination/identification, a Mightysil RP-4 GP column and a mobile phase of 25% (v/v) ethanol in water with a photodiode array detector were used. Average recoveries from milk samples spiked with 0.05, 0.1, 0.2, and 0.5 microg mL(-1) of each drug were >82%. The inter- and intra-assay variabilities were 2.0-3.1%. The practical detection limits for 7 SAs were 0.005-0.02 microg mL(-1). The total time and amount of solvent required for the analysis of one sample were <40 min and <6 mL of ethanol, respectively. No toxic solvents were used.
Furusawa
N
N
Faculty of Human Sciences, Osaka City University, Osaka, Japan. furusawa@life.osaka-cu.ac.jp
Kishida
K
K
D000818:Animals; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D004784:Environmental Monitoring; D005506:Food Contamination / Q000032:analysis; D008892:Milk / Q000592:standards*; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis*; D014462:Ultrafiltration
D013449:Sulfonamides