In vitro assessment of intestinal IGF-I stability.
Insulin-like growth factor (IGF-I) is a 7648-Da polypeptide consisting of 70 amino acids. Clinically, IGF-I might be used in type II diabetes, which requires a life-long treatment. Therefore, delivery routes other than parenteral injections are highly desirable. For convenience, the peroral route is the most attractive. Therefore, in an attempt to answer the feasibility of oral delivery of IGF-I we examined the metabolism of this polypeptide in the gut in the presence of crude porcine pancreatic enzymes (CPPE) and flushings of the small and large intestine from pig, rat, and dog. Moreover, incubation studies with purified pancreatic enzymes that are present in the intestine were performed to determine the most active enzymes responsible for the intestinal cleavage of IGF-I. IGF-I was mainly degraded by chymotrypsin (t(1/2) = 2.7 min) and trypsin (t(1/2) = 34.6 min), whereas in the presence of aminopeptidase M and carboxypeptidase A IGF-I was stable up to 90 min. IGF-I was degraded in flushings from the jejunum, ileum, and colon. However, there were no significant differences in the stability of IGF-I between the examined intestinal segments. The addition of serine protease inhibitors such as a combination of aprotinin, soybean trypsin inhibitor, and Nalpha-p-tosyl-L-lysine chloromethyl ketone (TLCK), as well as casein profoundly improved the stability. Because we were able to improve the stability of IGF-I in vitro in all species at the same degree we speculate that a similar extension of half-life might also be possible in the human intestinal system.
Anderle
Pascale
P
Department of Applied BioSciences, ETH Zurich, CH-8057 Zurich, Switzerland. anderle@cgl.ucsf.edu
Langguth
Peter
P
Rubas
Werner
W
Merkle
Hans P
HP
D000818:Animals; D002364:Caseins / Q000378:metabolism; D004285:Dogs; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005243:Feces / Q000737:chemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000493:pharmacokinetics; D007420:Intestine, Large / Q000201:enzymology / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D012697:Serine Endopeptidases / Q000378:metabolism; D013552:Swine
D002364:Caseins; D004791:Enzyme Inhibitors; D007334:Insulin-Like Growth Factor I; D012697:Serine Endopeptidases