[Study on nuclear DNA content and image cytometry in cervical cancer and precursor lesions].
OBJECTIVE
The aim of this research was to study the biological and clinical features of cervical cancer and precursor lesions.


METHODS
Nuclear DNA was analyzed by image cytometry (ICM) in 125 embedded tissue 5 microns sections stained with Feulgen stain. Samples included normal cervical squamous epithelium (n = 11), cervical intraepithelial neoplasia I (CIN I) (n = 22), CINII (n = 17) and CINIII (n = 13), cervical neoplasm (n = 62).


RESULTS
The mean DNA content, nuclear area increased progressively from normal cervical epithelium, CINI, CINII, CINIII to invasive squamous carcinoma. Statistical analysis revealed significant difference (P < 0.05, < 0.01). Histograms of cellular DNA ploidy distribution showed different pattern, where 9 components (9C) cells were observed in cervical cancer and CINIII. No differences in DNA mean ploidy (DMP), DNA index (DI) and nuclear area were observed between cervical neoplasm.


CONCLUSIONS
It is increased progressively from normal cervical epithelium to cancer, in which CINIII is turning point.
Jiao
Y
Y
Department of Obstetrics and Gynecology, 466 Hospital of People's Liberation Army, Beijing 100089, China.
Zhou
X
X
Ma
S
S
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D019044:Image Cytometry; D007091:Image Processing, Computer-Assisted; D008875:Middle Aged; D011230:Precancerous Conditions / Q000737:chemistry* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000737:chemistry* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000737:chemistry* / Q000473:pathology
D004273:DNA, Neoplasm