[Sequence-determined conformational changes in the coding region of the promoter DNA on transcription complex formation].
Chemical footprinting was used to study the spatial structure of bacteriophage T7 promoter D upon formation of the transcriptionally active complex with Escherichia coli RNA polymerase. Enzyme binding was shown to induce conformational changes in sites located at positions 43 and 57, several helix turns away from the transcription start. This was the first finding of a structural deformation induced by assembly of the transcription complex. The deformation was associated with specific features of the promoter nucleotide sequence, and suggested high cooperativity in the organization of the transcription complex and substantial energy perturbations caused by the enzyme.
Masulis
I S
IS
Chasov
V V
VV
Kostianitsyna
E G
EG
Purtov
Iu A
IuA
Ozolin'
O N
ON
D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003062:Codon; D004279:DNA, Viral / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D003062:Codon; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases
Determinirovannye nukleotidnoĭ posledovatgel'nost'iu konformatsionnye izmeneniia pri-promotorno DNK v kodiruiushcheĭ oblasti pri obranzovanii transkriptsionnogo kompleksa.