Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris.
The secretion signals of Saccharomyces cerevisiae alpha mating factor, human midkine itself, and Pichia pastoris acid phosphatase, were tried for the expression of human midkine under the control of the AOX1 gene promoter in P. pastoris. Approximately 28 mg/l, 1.5 mg/l, and 0.2 mg/l of midkine were secreted by using the a mating factor pre-pro-sequence, the midkine signal sequence, and the phosphatase signal sequence in flask cultures, respectively.
Murasugi
A
A
Meiji Cell Technology Center, Meiji Milk Products Co., Ltd., Odawara, Kanagawa, Japan. AKIRA_MURASUGI@MEIJI-MILK.COM
Tohma-Aiba
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016207:Cytokines; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D000078792:Midkine; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics; D010843:Pichia / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins; D021382:Protein Sorting Signals / Q000235:genetics* / Q000502:physiology; D014170:Transformation, Genetic / Q000502:physiology; D000099188:Alternative Oxidase
D002352:Carrier Proteins; D016207:Cytokines; D018076:DNA, Complementary; D000078792:Midkine; D024101:Mitochondrial Proteins; D009414:Nerve Growth Factors; D010088:Oxidoreductases; D010940:Plant Proteins; D021382:Protein Sorting Signals; D000099188:Alternative Oxidase