Polymorphism of human platelet alloantigen in Chinese patients with acute myocardial infarction and acute ischemic stroke.
OBJECTIVE
To investigate the gene frequencies of 5 major human platelet alloantiqens (HPA 1-5) in Chinese population and to assess if polymorphism of HPA was associated with Chinese acute myocardial infarction (AMI) and acute ischemic stroke (AIS).


METHODS
HPA 1-5 genotyping was performed by PCR using allele specific primers and restriction enzyme digestion based on PCR products in 95 AMI cases, 188 AIS cases and 270 normal controls. Gene frequency distribution was tested by Hardy-Weinberg equilibrium and comparison of HPA gene frequencies between the patient and control groups by chi 2 test.


RESULTS
The gene frequencies of HPA 1-5 were the followings: HPA1a: 91%; 1b: 9%; HPA 2a: 94%; 2b: 6%; HPA 3a: 83%; 3b: 17%; HPA 4a: 98%; 4b: 2%; HPA5a: 97%; 5b: 3%. We found there were no significant differences in HPA 1-5 gene frequencies between AMI patients and normal controls. In AIS patients group HPA-2a allele frequency was significant higher than in controls, but this allele gene frequency in two groups (0.94 and 0.99) was very close and too many subjects in these two groups were overlapped. Otherwise no differences was found in other 4 HPA systems between cases and controls.


CONCLUSIONS
Polymorphism of HPA were not inherited risky factors and not associated with chinese arterial thrombotic diseases such as AMI and AIS.
Chen
F
F
Department of Hematology, Xiang Ya Hospital, Hunan Medical University, Changsha 410008, China. xychenfp@public.cs.hn.cn
Jian
Z
Z
Xie
Q
Q
Pu
X
X
Xiao
B
B
Han
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016824:Antigens, Human Platelet / Q000235:genetics*; D001792:Blood Platelets / Q000276:immunology*; D002545:Brain Ischemia / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics* / Q000276:immunology; D011110:Polymorphism, Genetic
D016824:Antigens, Human Platelet