title: Ion transport in roots: measurement of fluxes using ion-selective microelectrodes to characterize transporter function.
abstract: The transport of mineral ions into and out of tissues and cells is central to the life of plants. Ion transport and the plasma membrane transporters themselves have been studied using a variety of techniques. In the last 15 years, measurement of specific ion fluxes has contributed to the characterization of transport systems. Progress in molecular genetics is allowing gene identification and controlled expression of transporter molecules. However the molecular expression of transporter gene products must be characterized at the functional level. The ion-selective microelectrode technique to measure specific ion fluxes non-invasively is ideally suited to this purpose. This technique, its theory, its links with others and its application and prospects in plant science, are discussed. Ions studied include hydrogen, potassium, sodium, ammonium, calcium, chloride and nitrate. Applications discussed include: solute ion uptake by roots; gravitropism and other processes in the root cap, meristematic and elongation zones; Nod factor effect on root hairs; osmotic and salt stresses; oscillations; the effects of light and temperature. Studies have included intact roots, leaf mesophyll and other tissues, protoplasts and bacterial biofilms. A multi-ion capability of the technique will greatly assist functional genomics, particularly when coupled with imaging techniques, patch clamping and the use of suitable mutants.
authors.affiliation: School of Mathematics and Physics, University of Tasmania, Hobart, Australia. ian.newman@utas.edu.au
authors.forename: I A
authors.initials: IA
authors.lastname: Newman
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels; D017136:Ion Transport; D017736:Ion-Selective Electrodes; D008839:Microelectrodes; D008903:Minerals / Q000493:pharmacokinetics*; D018521:Plant Physiological Phenomena; D018517:Plant Roots / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D008903:Minerals