title: Neural regeneration along longitudinal polyglactin sutures across short and extended defects in the rat sciatic nerve.
abstract: OBJECTIVE
  The authors have previously shown that longitudinal sutures without artificial tube support regeneration across a 7-mm gap in the rat sciatic nerve. In the present study, the authors compared this new approach with the use of autologous nerve grafts across short defects and examined whether the approach could be used to support regeneration across extended gaps and whether the interposition of a short nerve segment (the stepping-stone procedure) was applicable in this model.
  
  
  METHODS
  Longitudinal sutures were used to bridge 7- and 15-mm gaps in the rat sciatic nerve. Contralateral comparisons were made to nerve autografts in the 7-mm group and to sutures plus a short interposed nerve segment in the 15-mm group. Regeneration was evaluated at 2, 4, and 12 weeks by using immunocytochemical analysis for Schwann cells, neurofilament protein, and macrophages and at 12 weeks also by using histological examination, including morphometry in the distal tibial trunk and tetanic force measurements in the gastrocnemius muscle.
  
  
  CONCLUSIONS
  The authors found that the results of regeneration after repair with longitudinal polyglactin sutures across short defects were not significantly different from those produced by the use of autologous nerve grafts. Regeneration, although poor, occurred along sutures across extended gaps and was significantly enhanced by an interposed nerve segment acting as a Schwann cell resource in this model.
authors.affiliation: Department of Hand Surgery, Malmö University Hospital, Sweden. Peter.Scherman@hand.mas.lu.se
authors.forename: P
authors.initials: P
authors.lastname: Scherman
authors.forename: G
authors.initials: G
authors.lastname: Lundborg
authors.forename: M
authors.initials: M
authors.lastname: Kanje
authors.forename: L B
authors.initials: LB
authors.lastname: Dahlin
mesh_terms: D000818:Animals; D019245:Compressive Strength / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D008264:Macrophages / Q000473:pathology; D018482:Muscle, Skeletal / Q000473:pathology / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology*; D009417:Nerve Tissue / Q000473:pathology / Q000637:transplantation*; D016900:Neurofilament Proteins / Q000502:physiology; D011098:Polyglactin 910 / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000473:pathology; D012584:Sciatic Nerve / Q000293:injuries / Q000503:physiopathology* / Q000601:surgery*; D013536:Suture Techniques; D014182:Transplantation, Autologous
chemical_list: D016900:Neurofilament Proteins; D011098:Polyglactin 910