title: Mitogenic stimulation in isoproterenol treated cells of dictyostelium discoideum.
abstract: Amoebae of the cellular slime mould Dictyostelium discoideum showed stimulated mitogenic activity when exposed to 200 microM isoproterenol, an activator of adenyl cyclase, for 30 min. Approximately 40% increase in cell proliferation was found at 48 h after isoproterenol treatment. A faster and larger plaque formation as well as higher uptake of FITC-labelled E. coli indicates greater phagocytotic activity in the treated cells. A concurrent increase in DNA and protein syntheses was also recorded in the treated cells. Administration of 400 microM caffeine or 200 microM (+) propranolol brought down the isoproterenol-induced elevation in the cell division rate to control levels. These results are discussed in relation to a precocious activation of adenyl cyclase in the treated cells leading to a transient but significant increase in cell division in this organism.
authors.affiliation: School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Kumar
authors.forename: Samar
authors.initials: S
authors.lastname: Chatterjee
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002455:Cell Division; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004023:Dictyostelium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007545:Isoproterenol / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology*; D010587:Phagocytosis; D011433:Propranolol / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
chemical_list: D000319:Adrenergic beta-Antagonists; D000697:Central Nervous System Stimulants; D008934:Mitogens; D002110:Caffeine; D004247:DNA; D011433:Propranolol; D000262:Adenylyl Cyclases; D007930:Leucine; D007545:Isoproterenol; D013936:Thymidine