Development of enzyme biosensor based on pH-sensitive field-effect transistors for detection of phenolic compounds.
This article describes a biosensor based on pH-sensitive field-effect transistors (pH-FETs) as transducer, and immobilised enzyme tyrosinase as biorecognition element, which was used for the determination of phenolic compounds in water solutions. The biologically active membrane was formed by cross-linking of tyrosinase with bovine serum albumin (BSA) in saturated glutaraldehyde (GA) vapours on the sensitive transducer surface. The main analytical characteristics were studied under different conditions as well as the possibility to optimise these working parameters. Different factors such as the pH of immobilisation, the enzyme loading, the time of exposition to glutaraldehyde vapours were investigated in regards to the influence on sensitivity, limit of detection, dynamic range, and operational and storage stability.
Dzyadevych
S V
SV
Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, 150 Zabolotnogo St., Kiev 03143, Ukraine. dzyad@yahoo.com
Mai Anh
T
T
Soldatkin
A P
AP
Duc Chien
N
N
Jaffrezic-Renault
N
N
Chovelon
J-M
JM
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D014442:Monophenol Monooxygenase / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D011199:Potentiometry; D012680:Sensitivity and Specificity
D004800:Enzymes, Immobilized; D010636:Phenols; D014442:Monophenol Monooxygenase