[Effect of replenishing liver and kidney, supplementing qi and activating blood circulation recipe on proliferation and relevant gene expression of vascular smooth muscle cells].
OBJECTIVE
To study the effect of replenishing Liver and Kidney, supplementing Qi and activating blood circulation recipe (TCM) on the proliferation and relevant gene expression of vascular smooth muscle cells (VSMC).


METHODS
Using 3H-TdR incorporation to investigate the VSMC proliferation, using Northern blotting and reverse Polymerase Chain Reaction (RT-PCR) to detect the effect of serum with TCM on induced NO synthase (iNOS), endothelin (ET) induced c-jun and proliferation cell nucleus antigen (PCNA) gene expression.


RESULTS
The 3H-TdR incorporation value of VSMC treated by serum with TCM was lower than that of control serum (P < 0.05). Rich iNOS mRNA was detected in the VSMC stimulated by serum with TCM. Expressions of c-jun and PCNA gene induced by ET in the VSMC were significantly inhibited by serum with TCM.


CONCLUSIONS
The TCM could significantly inhibit VSMC proliferation.
Han
M
M
Institute of Basic Medical Sciences, Hebei Medical University, Shijiazhuang (050017).
Wen
J
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat