title: Evaluation of an automated clean-up system for the isotope-dilution high-resolution mass spectrometric analysis of PCB, PCDD, and PCDF in food.
abstract: An automated clean-up system was evaluated for the simultaneous analysis of polychiorinated dibenzo-p-dioxins (PCDD), dibenzofurans (PCDF), and biphenyls (PCB) in different foods. In addition to the seventeen 2,3,7,8-substituted PCDDIPCDF and four non-ortho PCB, by use of the clean-up system studied, it was possible to collect the eight mono-ortho and two di-ortho PCB and the seven indicator PCB in two separate fractions during the same clean-up run. The study was first performed using standard mixtures containing PCDD, PCDF and PCB, and a certified reference material. The recoveries of the 13C-labeled compounds ranged from 51 to 90%, indicating that the PCDD, PCDF, and PCB clean-up worked satisfactorily. Next, the automated system for PCDD, PCDF, and PCB analysis was evaluated for foods such as milk, egg, meat (beef, chicken, and pork), mussel, and olive oil. The recoveries of the 13C-labeled compounds ranged from 40 to 120% for PCB and from 57 to 113% for PCDD/ PCDF, meeting the requirements of well accepted methods. Thus, the automated clean-up system studied is a suitable alternative to conventional clean-up methods.
authors.affiliation: Dept Ecotechnologies, IIQAB-CSIC, Barcelona, Spain. eeeeco@cid.csic.es
authors.forename: E
authors.initials: E
authors.lastname: Eljarrat
authors.forename: J
authors.initials: J
authors.lastname: Sauló
authors.forename: A
authors.initials: A
authors.lastname: Monjonell
authors.forename: J
authors.initials: J
authors.lastname: Caixach
authors.forename: J
authors.initials: J
authors.lastname: Rivera
mesh_terms: D001331:Automation; D001572:Benzofurans / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002250:Carbon Radioisotopes; D005591:Chemical Fractionation; D004781:Environmental Exposure / Q000032:analysis / Q000517:prevention & control; D005502:Food / Q000592:standards; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D013058:Mass Spectrometry; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D011849:Radioactive Tracers; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D001572:Benzofurans; D002250:Carbon Radioisotopes; D000072317:Polychlorinated Dibenzodioxins; D011849:Radioactive Tracers; C023614:dibenzofuran; D011078:Polychlorinated Biphenyls