title: In-vitro cultivation of normal human oral keratinocytes.
abstract: OBJECTIVE
  To establish a method for culturing normal human oral keratinocytes.
  
  
  METHODS
  Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.
  
  
  RESULTS
  Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody.
  
  
  CONCLUSIONS
  Human oral keratinocytes have been successfully grown in serial culture.
authors.affiliation: Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Shang
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D007633:Keratins / Q000032:analysis; D009061:Mouth Mucosa / Q000166:cytology*
chemical_list: D007633:Keratins