[Expression of CCR5 and its ligand in joint fluid and synovium of patients with rheumatoid arthritis].
OBJECTIVE
To investigate the expression and distribution of C-C chemokine recepter 5 (CCR5) and its ligand in peripheral blood, synovium, and synovial fluid so as to study the mechanism of selective accumulation of Th1 cells in rheumatoid joints.


METHODS
Synovial fluid mononuclear cells (SFMC) and peripheral blood mononuclear cells (PBMC) were isolated from 15 patients with active rheumatoid arthritis (RA). The expressions of intracellular cytokines, CCR5 and CXCR3 on cytomembraine of SFMCs and PBMCs, and MIP-1 immunofluorescence labelling, and flow cytometry.


RESULTS
(1) The selective pattern of intracellular cytokines in SFMCs was drifted towards Th1 subset. (2) The expression rate of CCR5 was 52% Y 8%, the expression rate of CXCR3 was 61% Y 12% in synovial fluid, significantly higher than those of PBMCs. (3) In RA tissues, most of the infiltrating T cells (especially CD4+ T cells), monocyres/microphages, and B cells expressed macrophage inflammatory protein, ligand of CCR5.


CONCLUSIONS
Infiltrating T cells (especially CD4+ T((MIP)-1 cells), monocyres/microphages, and B cells in the synovium of inflammatory, RANTES, and other cytokines which cause the accumulation of CCR5+ Th1 cells.
Zhu
P
P
Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Wang
Y
Y
Fan
C
C
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D054407:Chemokine CCL4; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D019402:Macrophage Inflammatory Proteins / Q000032:analysis* / Q000096:biosynthesis; D051379:Mice; D008875:Middle Aged; D019713:Receptors, CCR5 / Q000032:analysis*; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry*; D013583:Synovial Membrane / Q000737:chemistry*
C515187:CXCR3 protein, human; D054407:Chemokine CCL4; C515188:Cxcr3 protein, mouse; D019402:Macrophage Inflammatory Proteins; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine