Liquid chromatographic method for the analysis of tocopherols in malt sprouts with supercritical fluid extraction.
A simple, specific and sensitive high-performance liquid chromatographic method has been developed for the determination of tocopherols in malt sprouts. A supercritical fluid extraction (SFE) procedure was used to isolate tocopherols from the vegetal matrix before quantitative analysis. The analytes were separated on a Zorbax reversed-phase column using methanol-water as mobile phase and quantified by measuring its fluorescence at lambda(em)=328 nm after excitation of the analytes at lambda(exc)=303 nm. The limits of detection for alpha-, gamma- and delta-tocopherols were 0.04, 0.05, and 0.05 microg/ml, respectively. The calibration graphs of the method were linear from 0.1 to 1.5, 0.2 to 2.5, and 0.2 to 2.0 microg/ml, for alpha-, gamma- and delta-tocopherols, respectively. This SFE and HPLC procedure is simple, precise and accurate for the determination of tocopherols in malt sprouts.
Carlucci
G
G
Department of Chemistry, Chemical Engineering and Materials, University of L'Aquila, Coppito (AQ), Italy. carlucci@univaq.it
Mazzeo
P
P
Del Governatore
S
S
Di Giacomo
G
G
Del Re
G
G
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D024505:Tocopherols / Q000032:analysis*
D024505:Tocopherols