[Gene therapy with tumor suppressor gene p53 and(or) p16 on the nude mice models of NSCLC in vivo].
OBJECTIVE
To investigate the effect of tumor suppressor gene p53 and(or) p16 treatment on the nude mice models of non-small cell lung cancer (NSCLC).


METHODS
Nude mice were injected subcutaneously with NSCLC cell line A549. 25 nude mice were randomly divided into 5 groups (control, SA, p53 gene, p16 gene, p53 + p16 genes), p53 and(or) p16 genes mediated by stearylamine/DOPE (SA liposome) were injected intratumorally alone or jointly. The size of tumor and survival period of nude mice were measured after treatment.


RESULTS
p53 and(or) p16 genes can obviously inhibit the growth of tumor. The difference was significant between control and treated groups, among which the combined p53 and p16 genes enhance the inhibiting effect more markedly. The survival period of tumor-bearing nude mice was prolonged after transfecting p53 gene or p16 gene alone, p53 and p16 genes jointly can prolong the survival period significantly.


CONCLUSIONS
Tumor suppressor gene p53 and(or) p16 in the replacement therapy of NSCLC are of potential clinical significance. The combination of p53 and p16 gene may have a greater antitumoral effect in vivo.
Wu
W
W
Molecular Medical Center, Second Affiliated Hospital, Jiangxi Medical College, Nanchang 330006, China.
Zhang
X
X
Qi
X
X
D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D018076:DNA, Complementary / Q000008:administration & dosage / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D023041:Xenograft Model Antitumor Assays
D019941:Cyclin-Dependent Kinase Inhibitor p16; D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53