title: Increased p50/p50 NF-kappaB activation in human papillomavirus type 6- or type 11-induced laryngeal papilloma tissue.
abstract: We have observed elevated NF-kappaB DNA-binding activity in nuclear extracts from human papillomavirus type 6- and 11-infected laryngeal papilloma tissues. The predominant DNA-binding species is the p50/p50 homodimer. The elevated NF-kappaB activity could be correlated with a reduced level of cytoplasmic IkappaBbeta and could be associated with the overexpression of p21(CIP1/WAF1) in papilloma cells. Increased NF-kappaB activity and cytoplasmic accumulation of p21(CIP1/WAF1) might counteract death-promoting effects elicited by overexpressed PTEN and reduced activation of Akt and STAT3 previously noted in these tissues.
authors.affiliation: Department of Pediatrics, Long Island Jewish Medical Center, New Hyde Park, New York, USA.
authors.forename: Ivana
authors.initials: I
authors.lastname: Vancurova
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
authors.forename: Veronika
authors.initials: V
authors.lastname: Miskolci
authors.forename: Shishinn
authors.initials: S
authors.lastname: Sun
mesh_terms: D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D006801:Humans; D020672:I-kappa B Proteins; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit; D010212:Papilloma / Q000378:metabolism* / Q000473:pathology; D027383:Papillomaviridae / Q000502:physiology*; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D051996:Transcription Factor RelA; D014412:Tumor Virus Infections / Q000378:metabolism* / Q000473:pathology
chemical_list: C493956:CDKN1A protein, human; D002457:Cell Extracts; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004268:DNA-Binding Proteins; C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D051996:Transcription Factor RelA; D004247:DNA