[The use of chitosan solutions for defatting and clarification of protein hydrolysates].
The possibility of the use of small amounts of chitosan for defatting and clarification of protein solutions prepared by enzymatic hydrolysis was tested. The following treatment conditions were shown to be optimal: chitosan concentration range, from 1.0 to 1.5 g per kg raw weight; pH of precipitation medium, from 8.0 to 8.5; and duration of incubation of protein hydrolysate solution with chitosan, less than 1 h. The hydrolysate defatting grade was found to depend on the degree of chitosan deacetylation. A possible mechanism of the chitosan-induced effects was suggested. The use of chitosan allows the mass fraction of enzyme protein hydrolysates to be reduced fourfold to fivefold.
Novikov
Iu V
IuV
Knipovich Polar Institute of Marine Fisheries and Oceanography (PINRO), Murmansk, 183763 Russia.
Mukhin
V A
VA
D000107:Acetylation; D000818:Animals; D003386:Brachyura; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry*; D048271:Chitosan; D004064:Digestive System / Q000201:enzymology; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry*; D012996:Solutions
D008055:Lipids; D011492:Protein Hydrolysates; D012996:Solutions; D002686:Chitin; D048271:Chitosan
Ispol'zovanie rastvorov khitozana dli obezzhirivaniia i osvetleniia belkovykh gidrolizatov.