[Isolation and preliminary characterization of cryptic plasmids from Erwinia carotovora].
Of the fifty-two Erwinia carotovora strains studied, sixteen were found to contain extrachromosomal DNA (plasmids) from 2.5 to 129 kbp in size. Some E. carotovora strains bore two to five different plasmids. Experiments showed that the cryptic plasmids of erwinia are not responsible for their resistance to antibiotics and are not involved in the synthesis of macromolecular colicin-like carotovoricins. At the same time, one of the E. carotovora strains, 13A, augmented the production of carotovoricin after curing from one of its plasmids, 47.7-kbp pCA 6-2. Three E. carotovora subsp. carotovora strains and one E. carotovora subsp. atroseptica strain contained large 129-kbp plasmids, which may play a role in the ecology of phytopathogenic pectinolytic erwinia.
Tovkach
F I
FI
Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 03143 Ukraine. feodorit@mail.ru
D004269:DNA, Bacterial / Q000145:classification / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D016973:Pectobacterium carotovorum / Q000235:genetics*; D010957:Plasmids / Q000145:classification / Q000302:isolation & purification*
D004269:DNA, Bacterial
Vydelenie i predvaritel'naia kharakteristika kripticheskikh plazmid Erwinia carotovora.