[Effects of laminin on the invasive and metastatic behaviors of human salivary adenoid cystic carcinoma cell lines].
OBJECTIVE
To study the effect of laminin (LN) on invasive and metastastic behaviors of salivary adenoid cystic carcinoma (Acc) cell lines.


METHODS
Immunohistochemistry, Boyden chamber in vitro motility, and adhesion test were used. LN and LNR (LN receptor) distributions in Acc-2 and Acc-M cells were studied with immunohistochemical staining.


RESULTS
There were less LN and LNR found in Acc-2, but much more LN and LNR were present in the highly metastatic clone Acc-M. With the presence of exgenous LN, Acc-2 became activated showing a higher migration rate. The cell adhesion test showed that Acc-2 had stronger ability of adhesion than Acc-M with the presence of LN.


CONCLUSIONS
The result suggests LN and LNR may play a vitral role in the invasion and metastasis of Acc.
Shi
H
H
Ganquan Hospital, Shanghai Tiedao University, Shanghai 200065.
He
R
R
Zhou
Z
Z
D000818:Animals; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D017474:Receptors, Laminin / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D007797:Laminin; D017474:Receptors, Laminin