title: Modulation of BmKAS-1 and BmK1-3-2 to sodium channel in rat dorsal root ganglion neurons.
abstract: OBJECTIVE
  To investigate what effects BmKAS-1 (a polypeptide purified from the Chinese scorpion Buthus martensi Karsch [BmK] and named as BmK activator of skeletal-muscle ryanodine receptor) and its upstream mixture BmK1-3-2 have on Na+ channels in dorsal root ganglion (DRG) small diameter neurons.
  
  
  METHODS
  The whole-cell patch-clamp technique was used to investigate the effects of BmKAS-1 and BmK1-3-2 on Na+ current in rat small diameter DRG neurons.
  
  
  RESULTS
  About 50% peak Na+ current was suppressed by 10 micrograms/ml of BmK1-3-2. 1.62 micrograms/ml of BmKAS-1 also blocked 50% peak Na+ current, and there was an obvious dose-dependent relationship.
  
  
  CONCLUSIONS
  Both BmK1-3-2 and BmKAS-1 have a blocking effect on Na+ channels, and this may one of the mechanisms for the analgetic effect of BmK1-3-2 and BmKAS-1.
authors.affiliation: Department of Neurotoxicology, Applied Institute of Toxicology, Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China. neutoxi@njmu.edu.cn
authors.forename: H
authors.initials: H
authors.lastname: Xiao
authors.forename: X
authors.initials: X
authors.lastname: Mao
authors.forename: Z
authors.initials: Z
authors.lastname: Tan
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.forename: Z
authors.initials: Z
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D010455:Peptides; D051381:Rats; D012604:Scorpion Venoms / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology
chemical_list: C119408:KAS-1 toxin, Buthus martensi; D010455:Peptides; D012604:Scorpion Venoms; D015222:Sodium Channels