Comparison of PCR, capture ELISA and immunoblotting for detection of Toxoplasma gondii in infected mice.
PCR was compared with capture ELISA and immunoblotting for the detection of Toxoplasma gondii in sera of acutely infected mice. One hundred animals were inoculated intraperitoneally with 5000 trophozoites of RH strain and five of them were killed every 3 h from 3 h to 21 h post infection (p.i.), and every day from day 1 to day 7 p.i.. No assay detected the parasite from 3 h p.i. to 15 h p.i. PCR was the most sensitive assay and detected the T. gondii from 18 h p.i., whereas the other assays detected it only from day 1.
Hafid
J
J
*Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02, France and †Unité d'Immunologie et de Physiologie, Département de Biologie, Faculté des Sciences et Techniques, Avenue A. El Khattabi, BP 618, 40000 Marrakech, Morocco.
Flori
P
P
*Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02, France and †Unité d'Immunologie et de Physiologie, Département de Biologie, Faculté des Sciences et Techniques, Avenue A. El Khattabi, BP 618, 40000 Marrakech, Morocco.
Raberin
H
H
*Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02, France and †Unité d'Immunologie et de Physiologie, Département de Biologie, Faculté des Sciences et Techniques, Avenue A. El Khattabi, BP 618, 40000 Marrakech, Morocco.
Tran Manh Sung
R
R
*Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Faculté de Médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02, France and †Unité d'Immunologie et de Physiologie, Département de Biologie, Faculté des Sciences et Techniques, Avenue A. El Khattabi, BP 618, 40000 Marrakech, Morocco.
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D015151:Immunoblotting / Q000379:methods*; D007274:Injections, Intraperitoneal; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
D016054:DNA, Protozoan