[Immunological mechanism of human alpha-interferon gene therapy for treatment of melonoma].
OBJECTIVE
To investigate the possibility of alpha-INF gene therapy for treatment of melonoma.


METHODS
An experimental model of fibroblast-mediated human alpha-IFN gene therapy was established for treatment of melonoma and the activity of NK and M phi was determined.


RESULTS
The NK and M phi activity was increasing significantly after the implantation of alpha-IFN secreted by cloning the fibroblast in vivo. Good therapeutic results could be achived if the NIH3T3-IFN-alpha+ cells were combined with the IL-2/AK.


CONCLUSIONS
The results has indicated that the fibroblast-mediated gene therapy could be good to use it for the treatment of human melonoma. More effective results could be achieved by using it in combination with IL-2.
Chen
M
M
Department of Plastic and Reconstructive Surgery, Changzheng Hospital, 2nd Military Medical University, Shanghai 200003, China.
Lin
Z
Z
Cao
X
X
D016475:3T3 Cells; D000818:Animals; D015316:Genetic Therapy; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
D016898:Interferon-alpha