Enzymatic and non-enzymatic reduction of brucine N-oxide by aldehyde oxidase and catalase.
1. Brucine N-oxide was reduced by aldehyde oxidase in rabbit liver cytosol in the presence of an electron donor, 2-hydroxypyrimidine, under anaerobic conditions. The flavoprotein purified from rabbit liver exhibited significant reductase activity in the presence of electron donors. 2. Brucine N-oxide was also reduced by rabbit liver cytosol and blood in the presence of both a reduced pyridine nucleotide and FAD under anaerobic conditions. The N-oxide reductase activities were inhibited by carbon monoxide and air. However, these activities were not abolished whe n liver cytosol and blood were boiled. Rabbit erythrocytes exhibited the reductase activity, but not plasma. 3. When liver cytosol or blood was separated by DEAE-cellulose column chromatography, the fractions with the reducing activity in the presence of both NADH and FAD also showed catalase activity. 4. Catalase catalysed the brucine N-oxide reduction in the presence of both NAD(P)H and FAD. Hematin also exhibited the reductase activity in the presence of both NAD(P)H and FAD. Photochemically reduced FAD was effective in the reduction instead of NAD(P)H and FAD. 5. Bricine N-oxide reduction proceeds via two routes in liver cytosol and blood. One is enzymatic reduction by aldehyde oxidase; the other is non-enzymatic reduction catalysed by the haem group of catalase in the presence of reduced flavin.
Takekawa
K
K
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Sugihara
K
K
Kitamura
S
S
Ohta
S
S
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001769:Blood / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D002848:Chromatography, DEAE-Cellulose; D003497:Cyclic N-Oxides / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005415:Flavins / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D011817:Rabbits; D013331:Strychnine / Q000031:analogs & derivatives* / Q000378:metabolism*
D003497:Cyclic N-Oxides; D005415:Flavins; D005182:Flavin-Adenine Dinucleotide; C083067:brucine N-oxide; D002374:Catalase; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; D013331:Strychnine