title: An evaluation of the impact of supplementation with antioxidant vitamins on oxidation stress parameters in children with chronic recurrent gastroduodenitis.
abstract: BACKGROUND
  The purpose of our research was to evaluate the impact of supplementation with antioxidative vitamins in children with chronic recurrent gastroduodenitis on the concentration of malon-dialdehyde (MDA) in plasma and erythrocytes, and on activity of the antioxidant enzymes: dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px).
  
  
  METHODS
  We studied 19 children (age from 13 to 16), diagnosed with chronic recurrent gastroduodenitis. The concentration of MDA was measured by Buege and Aust method, while the antioxidant enzyme activity was measured before and after a two-month course of supplementation with vitamins C and E: SOD by the Misra and Fridovich method, CAT by the Bears and Seizer method, and GSH-Px by the Paglia and Valentine method.
  
  
  RESULTS
  The average concentration of MDA in erythrocytes in the studied children was significantly higher (38.59 nM/gHb) than in control group (24.07 nM/gHb), but after supplementation was significantly reduced (p<0.05). The average SOD activity in the experimental group was significantly lower both before (885 U/gHb) and after supplementation (923.3 U/gHb) than in the control group (1240 U/gHb). The activity of the remaining enzymes did not differ significantly in experimental and control groups.
  
  
  CONCLUSIONS
  The increased erythrocyte concentration of MDA in children with chronic recurrent gastroduodenitis may point to the generation of active forms of oxygen due to the low activity of the anti-oxidant enzyme SOD. Supplementation with the antioxidant vitamins C and E in children with a low antioxidant potential and chronic recurrent gastroduodenitis reduces the generation of free oxygen radicals.
authors.affiliation: Department and Clinic of Pediatrics, Allergology, and Gastroenterology, Medical University, Bydgoszcz, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Bała
authors.forename: Mieczysława
authors.initials: M
authors.lastname: Czerwionka-Szaflarska
authors.forename: Gerard
authors.initials: G
authors.lastname: Drewa
authors.forename: Grazyna
authors.initials: G
authors.lastname: Mierzwa
mesh_terms: D000293:Adolescent; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase / Q000097:blood; D019587:Dietary Supplements; D004382:Duodenitis / Q000188:drug therapy*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase / Q000097:blood; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase