[Effect of cigarette smoke extract on E-cadherin expression of airway epithelial cells].
OBJECTIVE
To explore the possible mechanism of injury by smoking, and the effect of cigarette smoke extract (CSE) on E-cadherin (ECD) expression in airway epithelial cells (AEC) was observed.


METHODS
Mouse trachea and pig AEC were cultured in vitro. After CSE exposure, the expression of ECD in AECs was evaluated using immunohistochemical stain and image analysis.


RESULTS
ECD was distributed on the plasma membrane at the cell junctions of AECs. At 12th and 24th hour after CSE exposure, the ECD expression on membrane decreased, whereas the cytoplasmic expression increased (P < 0.01) as compared with the normal controls. The expression at 24th hour was higher than that at 12th hour (P < 0.01).


CONCLUSIONS
CSE decreases the membranous expression of ECD in AECs, but increases the cytoplasmic expression. The change of ECD expression may mediate the airway epithelial injuries by smoking.
Wang
X
X
Department of Pathology, College of Basic Medical Sciences, Tongji Medical University, Wuhan 430030.
Hao
T
T
Wu
R
R
D000818:Animals; D015820:Cadherins / Q000096:biosynthesis*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D013552:Swine; D014026:Nicotiana / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D015820:Cadherins; D014028:Tobacco Smoke Pollution