title: [Study on 515 anti-tumor recipe in inducing leukemic HL-60 cells apoptosis].
abstract: OBJECTIVE
  To observe the efficacy of 515 anti-tumor recipe on inducing human myeloid leukemic HL-60 cells apoptosis.
  
  
  METHODS
  The morphological change of apoptotic cells was observed by light microscopy and transmission electromicroscopy (TEM), and flow cytometry and TUNEL reaction to further confirm the apoptosis of HL-60 cells.
  
  
  RESULTS
  Typical apoptosis appeared such as marginal nuclei, chromatin condensation and nuclear fragmentation could be seen by light microscopy and TEM. The drug concentration was between 0.013-0.04 g/ml. The apoptosis rate is 30% in 0.04 g/ml group at the 72nd hour, but it was only 0.8% in the control group at the same time (P < 0.01).
  
  
  CONCLUSIONS
  The 515 anti-tumor recipe can induce apoptosis in HL-60 cells. The apoptosis rate increased as the time extended. There was an enhanced efficiency of apoptosis in a dose-dependent manner.
authors.affiliation: Shandong Provincial Hospital, Jinan (250021).
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Song
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal