Stably transfected ABCA1 antisense cell line has decreased ABCA1 mRNA and cAMP-induced cholesterol efflux to apolipoprotein AI and HDL.
Using a sensitive real time fluorescent PCR assay, ABCA1 mRNA levels were induced by approximately 50-70-fold following 8Br-cAMP treatment of the RAW264 murine macrophage cell line, concomitant with the induction of cholesterol efflux to apoAI and HDL. A stably transfected ABCA1 antisense cDNA cell line was created, which led to approximately 50-70% reductions in ABCA1 mRNA levels in basal and 8Br-cAMP-treated cells, and diminished to the same extent the 8Br-cAMP-mediated efflux of cholesterol to apolipoprotein AI and HDL. These data demonstrate that ABCA1 is necessary for the cAMP-induced lipid efflux to both apoAI and HDL.
Zheng
P
P
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Horwitz
A
A
Waelde
C A
CA
Smith
J D
JD
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D000242:Cyclic AMP; D016373:DNA, Antisense; D015536:Down-Regulation; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D016372:RNA, Antisense / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D014162:Transfection
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D016632:Apolipoprotein A-I; D016373:DNA, Antisense; D008075:Lipoproteins, HDL; D016372:RNA, Antisense; D012333:RNA, Messenger; D002784:Cholesterol; D000242:Cyclic AMP