Purification and some properties of an inhibitor for a yeast pleiotropic drug resistant pump from Kitasatospora sp. E-420.
A strain producing an inhibitor for pleiotropic drug resistant 5 (Pdr5) was isolated using our original screening system in yeast. The strain was classified and named as Kitasatospora sp. E-420. The purified inhibitor (molecular weight = 1,193 by FAB-MS) inhibited a Pdr5-mediated efflux of cycloheximide and cerulenin. The intracellular accumulation of a fluorescent dye, rhodamine 123, by the inhibitor was also confirmed. Some physicochemical data suggested that the Pdr5-specific inhibitor was different from an immunosuppressant, FK506, reported as the only inhibitor of Pdr5 in vivo.
Wanigasekera
A
A
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Matsugasaki, Japan.
Hiraga
K
K
Hamanaka
N
N
Oda
K
K
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors*; D000935:Antifungal Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors; D002569:Cerulenin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000037:antagonists & inhibitors*; D008970:Molecular Weight; D020112:Rhodamine 123 / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013056:Spectrophotometry, Ultraviolet; D013306:Streptomycetaceae / Q000737:chemistry*
D018528:ATP-Binding Cassette Transporters; D000935:Antifungal Agents; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; C092438:PDR5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D002569:Cerulenin; D020112:Rhodamine 123; D003513:Cycloheximide