title: Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis.
abstract: The fission yeast plo1(+) gene encodes a polo-like kinase, a member of a conserved family of kinases which play multiple roles during the cell cycle. We show that Plo1 kinase physically interacts with the anaphase-promoting complex (APC)/cyclosome through the noncatalytic domain of Plo1 and the tetratricopeptide repeat domain of the subunit, Cut23. A new cut23 mutation, which specifically disrupts the interaction with Plo1, results in a metaphase arrest. This arrest can be rescued by high expression of Plo1 kinase. We suggest that this physical interaction is crucial for mitotic progression by targeting polo kinase activity toward the APC.
authors.affiliation: The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK.
authors.forename: Karen M
authors.initials: KM
authors.lastname: May
authors.forename: Nicola
authors.initials: N
authors.lastname: Reynolds
authors.forename: C Fiona
authors.initials: CF
authors.lastname: Cullen
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanagida
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohkura
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D064196:Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome; D017124:Conserved Sequence; D029721:Drosophila Proteins; D008025:Ligases / Q000378:metabolism*; D008677:Metaphase; D008938:Mitosis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000235:genetics*; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
chemical_list: D064196:Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome; D029721:Drosophila Proteins; D021122:Protein Subunits; D029702:Schizosaccharomyces pombe Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D017346:Protein Serine-Threonine Kinases; D008025:Ligases