[Gene detection and localization by FISH with short DNA fragment cloned in plasmid].
OBJECTIVE
To establish a method of detecting and localizing newly cloned gene by FISH using short DNA fragment.


METHODS
Bio-14-dATP was incorporated into the target gene cloned in plasmid by nick translation followed by conventional FISH with some modification. By Means of this method, c-met, c-erbB2, 1A6, APC and Rb genes were examined in ovarian and gastric cancer cell lines.


RESULTS
1A6, a newly isolated gene was located at the long arm of one of the C group chromosomes. Oncogene c-met, c-erbB2 and trmor suppressor gene APC, Rb were shown to produce clear-cut hybridization signals in ovarian and gastric cancer cells.


CONCLUSIONS
Short DNA fragment cloned in plasmid is useful for studies of gene localization, gene deletion and amplification by FISH.
Zheng
S
S
Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University, Beijing 100034.
Qiao
H
H
Lu
G
G
D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis*; D017404:In Situ Hybridization, Fluorescence; D010957:Plasmids
D004247:DNA