[Effect of antioxidant histochrome preparation on the contractile function and metabolism of the isolated rat heart under conditions of "calcium paradox", ischemia, and reperfusion].
A comparative study of protective effect of a new antioxidant histochrome on isolated rat heart at calcium paradox and ischemia-reperfusion was carried out. Perfusion with Ca-containing solution after 10-min perfusion with Ca-free medium caused depletion of high energy phosphates, loss of myoglobin, uncoupling of mitochondria, increase of left ventricular diastolic pressure. Pretreatment with histochrome led to decrease of cardiomyocyte damage evaluated by myoglobin efflux in perfused solution, attenuation of decrease ATP and phosphocreatine, and coupling of mitochondria. This resulted in a decrease of left ventricular diastolic pressure at calcium paradox. Less effect of histochrome treatment was noted in the case of ischemia-reperfusion model. It was concluded that histochrome effectively prevents heart from abnormalities caused by calcium imbalance in heart.
Vinokurov
A A
AA
Voronezh State Medical Academy, Studencheskaya Street 10, Voronezh 394622, Russia.
Alabovskiĭ
V V
VV
Shul'zhenko
V S
VS
Ivanova
M V
MV
Lebedev
A V
AV
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D019069:Cell Respiration; D005110:Extracellular Space / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects*; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D009211:Myoglobin / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010725:Phosphocreatine / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar
D000227:Adenine Nucleotides; D000975:Antioxidants; D002316:Cardiotonic Agents; D009211:Myoglobin; D009285:Naphthoquinones; C103614:histochrome; D010725:Phosphocreatine; D002118:Calcium
Vliianie antioksidantnogo preparata gistokhrom na sokratitel'nuiu funktsiiu i metabolizm izolirovannogo serdtsa krysy v usloviiakh "kal'tsievogo paradoksa", ishemii i reperfuzii.