title: Change of major genotype of enterovirus 71 in outbreaks of hand-foot-and-mouth disease in Taiwan between 1998 and 2000.
abstract: Two outbreaks of hand-foot-and-mouth disease (HFMD) occurred in Taiwan between 1998 and 2000. Enteroviruses were isolated from a total of 1,892 patients in this laboratory during this period. Of the virus isolates, enterovirus 71 (EV71) was diagnosed in 44.4% of the patients (132 of 297) in 1998, 2% (13 of 646) in 1999, and 20.5% (195 of 949) in 2000. Genetic analyses of the 5'-untranslated and VP1 regions of EV71 isolates by reverse transcription-PCR and sequencing were performed to understand the diversity of EV71 in these outbreaks of HFMD. Most EV71 isolates from the 1998 epidemic belonged to genotype C, while only one-tenth of the isolates were genotype B. Interestingly, all EV71 isolates tested from 1999 to 2000 belonged to genotype B. This study indicated that two genogroups of EV71 capable of inducing severe clinical illness have been circulating in Taiwan. Furthermore, the predominant EV71 genotypes responsible for each of the two major HFMD outbreaks within the 3-year period in Taiwan were different.
authors.affiliation: Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. jrwang@mail.ncku.edu.tw
authors.forename: Jen-Ren
authors.initials: JR
authors.lastname: Wang
authors.forename: Yen-Chang
authors.initials: YC
authors.lastname: Tuan
authors.forename: Huey-Pin
authors.initials: HP
authors.lastname: Tsai
authors.forename: Jing-Jou
authors.initials: JJ
authors.lastname: Yan
authors.forename: Ching-Chuan
authors.initials: CC
authors.lastname: Liu
authors.forename: Ih-Jen
authors.initials: IJ
authors.lastname: Su
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D004196:Disease Outbreaks; D004770:Enterovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006232:Hand, Foot and Mouth Disease / Q000453:epidemiology / Q000821:virology; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013624:Taiwan / Q000453:epidemiology
chemical_list: D020121:5' Untranslated Regions; D036022:Capsid Proteins