BMP-12 gene transfer augmentation of lacerated tendon repair.
Bone morphogenetic protein (BMP) 12 is a recently discovered member of the human BMP family. It is the human homolog of mouse growth/differentiation factor (GDF)-7. Previously we reported that injection of mesenchymal progenitor cells transferred with the BMP-12 gene into the muscles of nude mice induced tendon-like tissue formation. In this study, we further investigated the effect of BMP-12 gene transfer on tendon cells. We observed that adenovirus mediated in vitro BMP-12 gene transfer into chicken tendon cells increased type I collagen synthesis. No change in alkaline phosphatase activity was observed following BMP-12 gene transfer. We also determined that BMP-12 gene transfer into a complete tendon laceration chicken model resulted in a two-fold increase of tensile strength and stiffness of repaired tendons, indicating improved tendon healing in vivo. We conclude that BMP-12 gene transfer is a promising procedure for improving the tendon repair process.
Lou
J
J
Department of Orthopaedic Surgery, Barnes-Jewish Hospital at Washington University, St. Louis, MO 63110, USA. louj@msnotes.wustl.edu
Tu
Y
Y
Burns
M
M
Silva
M J
MJ
Manske
P
P
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis; D022761:Gene Transfer, Horizontal; D015316:Genetic Therapy; D055412:Growth Differentiation Factors; D013708:Tendon Injuries / Q000503:physiopathology / Q000628:therapy*; D013710:Tendons / Q000502:physiology*; D013718:Tensile Strength; D016212:Transforming Growth Factor beta; D014945:Wound Healing
D019485:Bone Morphogenetic Proteins; C499470:GDF7 protein, human; D055412:Growth Differentiation Factors; D016212:Transforming Growth Factor beta; C086559:growth differentiation factor 7; D003094:Collagen; D000469:Alkaline Phosphatase