Determination of concentrations of sex steroids in blood plasma and semen of male dogs treated with delmadinone acetate or finasteride.
The concentrations of testosterone, 5 alpha-dihydrotestosterone, oestradiol and oestrone were determined in peripheral blood plasma and semen of male dogs. In an experimental study, three Beagles were treated once with delmadinone acetate (1 mg kg-1 body weight, i.m.) and three were submitted to oral applications of finasteride (1 mg kg-1 body weight) once a day for 3 weeks. In a clinical study, 51 dogs of different breeds were divided into four groups according to the total number of spermatozoa in ejaculates (normospermia, slight oligozoospermia, severe oligozoospermia and azoospermia). The testosterone concentrations were significantly lower in sperm-rich ejaculate fractions and prostatic secretions compared with blood plasma (P < 0.05). The lowest concentration of testosterone was found in prostatic fluid. Concentrations of 5 alpha-dihydrotestosterone were similar in blood plasma and sperm-rich fractions, and significantly lower in prostatic secretions (P < 0.05). The concentrations of oestradiol and oestrone did not differ between blood plasma and either ejaculate fraction. Significantly higher 5 alpha-dihydrotestosterone concentrations and significantly lower concentrations of oestradiol and oestrone were found in prostatic secretions from azoospermic ejaculates compared with prostatic secretions of normospermic and oligozoospermic ejaculates. Delmadinone acetate and finasteride caused reversible suppression of the secretory activity of the prostate gland. The application of delmadinone acetate led to a temporary alteration of maturation of epididymal spermatozoa.
Lange
K
K
Institute for Reproductive Medicine, School of Veterinary Medicine, Bünteweg 15, D-30559 Hannover, Germany.
Cordes
E K
EK
Hoppen
H O
HO
Günzel-Apel
A R
AR
D058891:5-alpha Reductase Inhibitors; D000818:Animals; D002715:Chlormadinone Acetate / Q000031:analogs & derivatives* / Q000627:therapeutic use; D003270:Contraceptive Agents / Q000627:therapeutic use; D013196:Dihydrotestosterone / Q000032:analysis / Q000097:blood; D004283:Dog Diseases / Q000188:drug therapy* / Q000378:metabolism; D004285:Dogs; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D004958:Estradiol / Q000032:analysis / Q000097:blood; D004970:Estrone / Q000032:analysis / Q000097:blood; D018120:Finasteride / Q000627:therapeutic use; D012739:Gonadal Steroid Hormones / Q000032:analysis* / Q000097:blood; D008297:Male; D009845:Oligospermia / Q000188:drug therapy* / Q000378:metabolism / Q000662:veterinary*; D011897:Random Allocation; D012661:Semen / Q000737:chemistry*; D013739:Testosterone / Q000032:analysis / Q000097:blood
D058891:5-alpha Reductase Inhibitors; D003270:Contraceptive Agents; D004791:Enzyme Inhibitors; D012739:Gonadal Steroid Hormones; D013196:Dihydrotestosterone; D002715:Chlormadinone Acetate; D004970:Estrone; D013739:Testosterone; D004958:Estradiol; D018120:Finasteride; C005928:delmadinone