[Study on apoptosis of fibroblasts from abnormal scars in media containing low level of serum and interleukin 1 beta].
OBJECTIVE
To investigate the effects of low serum and interleukin 1 beta on inducing apoptosis of fibroblasts from abnormal scars and normal skins.


METHODS
6 samples from keloid, hypertrophic scar and normal skin were collected respectively. Apoptosis of different fibroblasts in media containing low level of serum and interleukin 1 beta were studied by detecting Bax and Bcl-2 protein expressions and specific DNA ladder by means of cell culture, immunohistochemistry and agrose electrophoresis.


RESULTS
1. In low level of serum, hardly occurs apoptosis of fibroblasts from keloids in correspondence with no changes of the radio of Bax/Bcl-2 proteins, and apoptosis of fibroblasts from normal skins occurs in correspondence with increasing of the radio of Bax/Bcl-2 proteins, whereas, slightly occurs apoptosis of fibroblasts from hypertrophic scars in correspondence with no change of the radio of Bax/Bcl-2 proteins. 2. In media containing interleukin 1 beta apoptosis of all three kinds of fibroblasts occurs, whereas, apoptosis of fibroblasts from keloids and hypertrophic scars takes place more seriously than that from normal skins in correspondence with increasing of the Bax/Bcl-2 radio of fibroblasts from keloids, decreasing of the Bax/Bcl-2 radio of ones from normal skins and no change of the Bax/Bcl-2 radio of ones from hypertrophic scars.


CONCLUSIONS
Characters of different fibroblasts are different.
Guan
B
B
Department of Biology and Genetics, Medical College of Beijing University 100083, Beijing, China.
Xu
S
S
Bao
W
W
D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D002648:Child; D002921:Cicatrix / Q000378:metabolism / Q000473:pathology*; D017439:Cicatrix, Hypertrophic / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000473:pathology*
D007375:Interleukin-1