Quantitation of antihistamines in pharmaceutical preparations by liquid chromatography with a micellar mobile phase of sodium dodecyl sulfate and pentanol.
A reversed-phase liquid chromatographic procedure with a micellar mobile phase of sodium dodecyl sulfate (SDS), containing a small amount of pentanol, was developed for the control of 7 antihistamines of diverse action in pharmaceutical preparations (tablets, capsules, powders, solutions, and syrups): azatadine, carbinoxamine, cyclizine, cyproheptadine, diphenhydramine, doxylamine, and tripelennamine. The retention times of the drugs were <9 min with a mobile phase of 0.15M SDS-6% (v/v) pentanol. The recoveries with respect to the declared compositions were in the range of 93-110%, and the intra- and interday repeatabilities and interday reproducibility were <1.2%. The results were similar to those obtained with a conventional 60 + 40 (v/v) methanol-water mixture, with the advantage of reduced toxicity, flammability, environmental impact, and cost of the micellar-pentanol solutions. The lower risk of evaporation of the organic solvent dissolved in the micellar solutions also increased the stability of the mobile phase.
Gil-Agustí
M
M
Universitat Jaume I, Area de Química Analítica, Castelló, Spain.
Monferrer-Pons
L
L
Esteve-Romero
J
J
García-Alvarez-Coque
M C
MC
D002853:Chromatography, Liquid / Q000379:methods*; D004304:Dosage Forms; D006634:Histamine H1 Antagonists / Q000008:administration & dosage / Q000032:analysis*; D006801:Humans; D008823:Micelles; D000439:Pentanols; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
D004304:Dosage Forms; D006634:Histamine H1 Antagonists; D008823:Micelles; D000439:Pentanols; D004364:Pharmaceutical Preparations; D012967:Sodium Dodecyl Sulfate; C024999:n-pentanol