Detection of Chlamydia pneumonia DNA in nasopharyngolaryngeal swab samples from patients with rhinitis and pharyngolaryngitis with polymerase chain reaction.
OBJECTIVE
To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease.


METHODS
PCR assay was used to detect Chlamydia pneumonia specific Pst I 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis. C. pneumonia specific antibodies in sera were also assayed with microimmuno-fluoresence (MIF).


RESULTS
About 28% (49/175) of the patients were PCR positive and 25.7% (45/175) were MIF antibodies positive. The accordance rate of the two methods was 91.8%.


CONCLUSIONS
It is suggested that the C. pneumonia infection was common in this group of patients and the C. pneumonia Pst I 474 specific PCR was sensitive and specific for detecting C. pneumonia in pharyngolaryngitis or rhinitis and sinusitis.
Zhang
G
G
Department of Otolaryngology, Third Clinical College, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, China.
Ning
B
B
Li
Y
Y
D023521:Chlamydophila Infections / Q000382:microbiology / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D007827:Laryngitis / Q000382:microbiology*; D010612:Pharyngitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012220:Rhinitis / Q000382:microbiology*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial