title: Antibodies to hepatitis B surface antigen prevent viral reactivation in recipients of liver grafts from anti-HBC positive donors.
abstract: OBJECTIVE
  Liver donors with serological evidence of resolved hepatitis B virus (HBV) infection (HBV surface antigen (HBsAg) negative, anti-HBV core (HBc) positive) can transmit HBV infection to recipients. In the context of organ shortage, we investigated the efficacy of hepatitis B immunoglobulin (HBIG) to prevent HBV infection, and assessed the infectious risk by polymerase chain reaction (PCR) testing for HBV DNA on serum and liver tissue of anti-HBc positive donors.
  
  
  METHODS
  Between 1997 and 2000, 22 of 315 patients were transplanted with liver allografts from anti-HBc positive donors. Long term HBIG therapy was administered to 16 recipients. Four naive and two vaccinated patients received no prophylaxis.
  
  
  RESULTS
  Hepatitis B developed in the four HBV naive recipients without prophylaxis and in none of the vaccinated subjects. Among the 16 recipients receiving HBIG, one patient with residual anti-HBs titres below 50 UI/ml became HBsAg positive. The remaining 15 remained HBsAg negative and HBV DNA negative by PCR testing throughout a 20 month (range 4-39) follow up period. HBV DNA was detected by PCR in 1/22 donor serum, and in 11/21 liver grafts with normal histology. A mean of 12 months post-transplantation (range 1-23) HBV DNA was no longer detectable in graft biopsies from patients remaining HBsAg negative.
  
  
  CONCLUSIONS
  Anti-HBs antibodies may control HBV replication in liver grafts from anti-HBc positive donors, without additional antiviral drugs. These grafts are thus suitable either to effectively vaccinated recipients or to those who are given HBIG to prevent HBV recurrence.
authors.affiliation: Laboratoire de Virologie, Hôpital Paul Brousse, Université Paris-Sud UPRES 1596, 12 avenue Paul Vaillant Couturier, 94804 Villejuif, France. anne-marie.roque@pbr-ap-hop-paris.fr
authors.forename: A M
authors.initials: AM
authors.lastname: Roque-Afonso
authors.forename: C
authors.initials: C
authors.lastname: Feray
authors.forename: D
authors.initials: D
authors.lastname: Samuel
authors.forename: D
authors.initials: D
authors.lastname: Simoneau
authors.forename: B
authors.initials: B
authors.lastname: Roche
authors.forename: J-F
authors.initials: JF
authors.lastname: Emile
authors.forename: M
authors.initials: M
authors.lastname: Gigou
authors.forename: D
authors.initials: D
authors.lastname: Shouval
authors.forename: E
authors.initials: E
authors.lastname: Dussaix
mesh_terms: D000998:Antiviral Agents / Q000627:therapeutic use; D004279:DNA, Viral / Q000032:analysis; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000254:growth & development* / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use; D019259:Lamivudine / Q000627:therapeutic use; D016031:Liver Transplantation / Q000276:immunology / Q000379:methods*; D016133:Polymerase Chain Reaction; D016896:Treatment Outcome; D014775:Virus Activation / Q000502:physiology*
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D016756:Immunoglobulins, Intravenous; D019259:Lamivudine