[PI-3 kinase/AKT/PKB signal transduction pathway in kidneys mediates angiotensin II-induced hypertension].
BACKGROUND
The activation of PI-3 kinase/AKT/PKB signal transduction pathway has implicated in the cell growth regulation and proliferation.


OBJECTIVE
To determine a role of PI-3 kinase/AKT/PKB signal transduction pathway in the kidney of Angiotensin II-induced hypertensive rats.


METHODS
NZW (New Zealand White) rats have been infused by Angiotensin II using osmotic minipump for six days (n = 8). Control group was untreated rats (n = 6). PI-3 inase and AKT/PKB activities were measured in the presence or absence of different inhibitors.


RESULTS
Angiotensin II elevated mean arterial blood pressure (MABP) to 182 +/- 2 mm Hg (p < 0.001) vs untreated control rats (95 +/- 3 mm Hg). Ras-GTPase and PI-3 Kinase activities were elevated in angiotensin II-treated group. Ras inhibitors FPT III and BMS/191563 attenuated MABP to 122 +/- 2 and 127 +/- 4 mm Hg (p < 0.05) and abolished Ras-GTPase and PI-3 Kinase activities. AKT/PKB activity followed the PI-3 kinase activity.


CONCLUSIONS
PI-3 kinase/AKT/PKB signal transduction pathway in the kidney is activated and mediates Angiotesin II-induced hypertension.
Ljuca
F
F
Zavod za fiziologiju, Medicinski fakultet Univerziteta u Tuzli.
Nuhbegovic
S
S
Ljuca
D
D
Karamehic
J
J
Halilbasic
A
A
D000804:Angiotensin II; D000818:Animals; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D015398:Signal Transduction; D020703:ras GTPase-Activating Proteins / Q000378:metabolism
D000804:Angiotensin II; D019869:Phosphatidylinositol 3-Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D020703:ras GTPase-Activating Proteins; C494920:Akt1 protein, rat
PPI-3 kinaza/AKT/PKB put transdukcije signala u bubregu posreduje razvoj angiotenzin II-inducirane hipertenzije.