A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors.
For the design of a biosensor sensitive to steroidal glycoalkaloids, pH-Sensitive Field Effect Transistors as transducers and immobilised butyrylcholinesterase as a biorecognition element have been used. The total potato glycoalcaloids can be measured by this biosensor in the concentration range 0.5-100 microM with detection limits of 0.5 microM for alpha-chaconine and of 2.0 microM for alpha-solanine and solanidine, respectively. The responses of the developed biosensors were reproducible with a relative standard deviation of about 1.5% and 5% for intra- and inter-sensor responses (both cases, n=10, for an alkaloid concentration of 5 microM), respectively. Moreover, due to the reversibility of the enzyme inhibition, the same sensor chip with immobilised butyrylcholinesterase can be used several times (for at least 100 measurements) after a simple washing by a buffer solution and can be stored at 4 degrees C for at least 3 months without any significant loss of the enzymatic activity.
Korpan
Y I
YI
Ukrainian Centre of Biosensors, 54, Volodymyrska str., 01030, Kiev 30, Ukraine.
Volotovsky
V V
VV
Martelet
C
C
Jaffrezic-Renault
N
N
Nazarenko
E A
EA
El'skaya
A V
AV
Soldatkin
A P
AP
D000470:Alkaloids / Q000032:analysis*; D015374:Biosensing Techniques; D002091:Butyrylcholinesterase / Q000737:chemistry*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000737:chemistry
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D002091:Butyrylcholinesterase