title: How to overcome matrix effects in the determination of pesticides in fruit by HPLC-ESI-MS-MS.
abstract: A high-performance liquid chromatographic method, with electrospray ionisation tandem mass spectrometry (HPLC-ESI-MS-MS) for detection, has been developed for the determination of thiabendazole, carbendazime, and phenylurea pesticides in fruit matrices. During the validation process the method was tested for matrix effects, blanks, and the stability of the system. Considerable unspecific matrix effects in the ESI (+) process were detected by comparing standard calibration, and matrix calibration, although blank values were very low and the specific calibration functions showed only small standard deviations. This effect was overcome by using a more complex clean-up, i.e. an additional size-exclusion step.
authors.affiliation: European Community-Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Geel, Belgium. k.bester@infu.uni-dortmund.de
authors.forename: K
authors.initials: K
authors.lastname: Bester
authors.forename: G
authors.initials: G
authors.lastname: Bordin
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez
authors.forename: H
authors.initials: H
authors.lastname: Schimmel
authors.forename: J
authors.initials: J
authors.lastname: Pauwels
authors.forename: G
authors.initials: G
authors.lastname: Van Vyncht
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards
chemical_list: D010573:Pesticide Residues