title: Interaction between interleukin 1beta and endogenous neurokinin 1 receptor agonists in mediating plasma extravasation and neutrophil accumulation in the cutaneous microvasculature of the rat.
abstract: Interleukin 1beta (IL-1beta) is a potent mediator of neutrophil accumulation. Antidromic stimulation of the rat saphenous nerve leads to neurogenic oedema formation mediated by endogenous tachykinins. Here, we have investigated links between IL-1beta and the tachykinin 1 (NK(1)) receptors in microvascular events in rat skin. Saphenous nerve-induced plasma extravasation was not modulated by skin pretreatment with IL-1beta (3 pmol/site intradermally). In addition, the long-lasting antidromic electrical stimulation did not induce significant neutrophil accumulation in naive rat skin. By comparison, the effect of IL-1beta-induced neutrophil accumulation was significantly potentiated by co-stimulation of the ipsilateral saphenous nerve; an effect prevented by an NK(1) receptor antagonist (SR140333, 480 nmol/kg, i.v.). We conclude that IL-1beta-induced neutrophil accumulation can be influenced in a pro-inflammatory manner by ongoing neurogenic inflammation, of relevance to the sensory nerve input that occurs during ongoing inflammatory disease.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, Pécs University, Szigeti u. 12, 7643, Pécs, Hungary. erika.pinter@aok.pte.hu
authors.forename: Erika
authors.initials: E
authors.lastname: Pintér
authors.forename: Márta
authors.initials: M
authors.lastname: Thán
authors.forename: D Quyen
authors.initials: DQ
authors.lastname: Chu
authors.forename: Caraline
authors.initials: C
authors.lastname: Fogg
authors.forename: Susan D
authors.initials: SD
authors.lastname: Brain
mesh_terms: D000818:Animals; D004487:Edema / Q000378:metabolism; D004558:Electric Stimulation; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D008833:Microcirculation / Q000502:physiology; D009443:Neuritis / Q000378:metabolism; D064729:Neurokinin-1 Receptor Antagonists; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology; D010949:Plasma / Q000378:metabolism; D011812:Quinuclidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000819:agonists / Q000378:metabolism*; D012584:Sciatic Nerve / Q000502:physiology; D011984:Sensory Receptor Cells / Q000502:physiology; D012867:Skin / Q000098:blood supply* / Q000276:immunology / Q000294:innervation*; D015320:Tachykinins / Q000378:metabolism
chemical_list: D007375:Interleukin-1; D064729:Neurokinin-1 Receptor Antagonists; D010880:Piperidines; D011812:Quinuclidines; D018040:Receptors, Neurokinin-1; D015320:Tachykinins; C085215:SR 140333