title: Identification of principal constituents in enzymatically hydrolyzed coix extract.
abstract: The structural elucidation of the main constituents in enzymatically hydrolyzed coix extract, a natural food preservative, was carried out. After peracetylation, five compounds, namely peracetylated forms of glucose, maltose, maltotriose, maltotetraose, and maltopentaose were isolated. The structures were determined by PFG HMQC and HMBC experiments. In addition, by using HPLC with an RI detector, the main components of this coix extract were identified as a mixture of oligosaccharides having one to seven glucose units coupled through alpha-(1-->4) linkages. Since this extract showed no antimicrobial activity, its preservative effect may be caused by its covering of the food surface, thereby blocking contact with air.
authors.affiliation: National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sugimoto
authors.forename: J
authors.initials: J
authors.lastname: Fukuda
authors.forename: K
authors.initials: K
authors.lastname: Takatori
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Maitani
mesh_terms: D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D005520:Food Preservatives / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D005520:Food Preservatives; D009844:Oligosaccharides; D010936:Plant Extracts