title: The effect of the transfusion of stored RBCs on intestinal microvascular oxygenation in the rat.
abstract: BACKGROUND
  Although it is known that the transfusion of stored RBCs does not always improve tissue O(2) consumption under conditions of limited tissue oxygenation, the efficiency of O(2) delivery to the microcirculation by stored RBCs has never been determined.
  
  
  METHODS
  In a rat hemorrhagic shock model, the effects of resuscitation with fresh or 28-day-old RBCs stored in CPD plasma, saline-adenine-glucose-mannitol, and CPDA-1 plasma were investigated. Systemic hemodynamic and intestinal oxygenation measures were monitored. Intestinal microvascular PO(2) was determined with the O(2)-dependent quenching of palladium-porphyrin phosphorescence, and the RBC deformability was measured with a Laser-assisted optic rotational cell analyzer.
  
  
  RESULTS
  Hemodynamic and oxygenation measures were significantly decreased during hemorrhagic shock. Intestinal oxygen consumption and mesenteric venous pO(2) were restored with the transfusion of both fresh and stored RBCs, except for CPD-stored RBCs. The intestinal microvascular pO(2) improved only with the transfusion of fresh RBCs. Deformability of the stored RBCs was significantly decreased.
  
  
  CONCLUSIONS
  In contrast to that of fresh RBCs, the transfusion of stored RBCs did not restore the microcirculatory oxygenation, possibly because of impaired O(2) unloading, but, except for CPD-stored RBCs, the storage-induced changes were not enough to impair intestinal VO(2) and mesenteric venous pO(2).
authors.affiliation: Department of Anesthesiology, Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: van Bommel
authors.forename: D
authors.initials: D
authors.lastname: de Korte
authors.forename: A
authors.initials: A
authors.lastname: Lind
authors.forename: M
authors.initials: M
authors.lastname: Siegemund
authors.forename: A
authors.initials: A
authors.lastname: Trouwborst
authors.forename: A J
authors.initials: AJ
authors.lastname: Verhoeven
authors.forename: C
authors.initials: C
authors.lastname: Ince
authors.forename: C P
authors.initials: CP
authors.lastname: Henny
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D019069:Cell Respiration; D004195:Disease Models, Animal; D004907:Erythrocyte Deformability; D017707:Erythrocyte Transfusion / Q000592:standards*; D004912:Erythrocytes / Q000502:physiology; D006439:Hemodynamics; D007422:Intestines / Q000098:blood supply; D008297:Male; D008833:Microcirculation / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000503:physiopathology / Q000628:therapy