Biological activities of carbohydrate-branched chitosan derivatives.
Two types of biological activities of the carbohydrate-branched chitosan derivatives were investigated. One is the specific interaction with lectins and bacterium. The other is activation of canine polymorphonuclear leukocyte (PMN) cells. The specific bindings of the L-fucose-branched chitosan derivative with Ulex europaeus agglutinin I (UEA-I) and the N-acetyl-D-glucosamine-branched chitosan derivative with Concanavalin A (Con A) were confirmed by a surface plasmon resonance technique. The specific aggregation of the fluorescence-labeled L-fucose-branched chitosan derivative with Pseudomonas aeruginosa was observed by fluorescent microscopic observation. The aggregation would be attributed to the specific binding between the L-fucose-branched chitosan derivative and PA-II receptor on the cell surface of P. aeruginosa. The influence of the chitosan derivatives on the active oxygen species generation from canine PMN cells was also investigated by the luminol-aided chemiluminescence method. The chemiluminescence responses depended on the degree of substitution and water solubility of the chitosan derivatives. The water-insoluble chitosan derivatives would stimulate the PMN cells by a phagocytosis mechanism, and the water-soluble ones would sensitize the PMN cells by a priming mechanism.
Morimoto
M
M
Department of Materials Science, Faculty of Engineering, Tottori University, Koyama, Tottori 680-8552, Japan.
Saimoto
H
H
Usui
H
H
Okamoto
Y
Y
Minami
S
S
Shigemasa
Y
Y
D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D048271:Chitosan; D004285:Dogs; D008856:Microscopy, Fluorescence; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D037121:Plant Lectins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012995:Solubility; D020349:Surface Plasmon Resonance
D000942:Antigens, Bacterial; D037121:Plant Lectins; D017382:Reactive Oxygen Species; C036850:Ulex europaeus lectins; D002686:Chitin; D048271:Chitosan