Generalized concentration dependence of globular protein self-diffusion coefficients in aqueous solutions.
The self-diffusion coefficients of globular proteins (myoglobin, bovine serum albumin, barstar, lysozyme) in aqueous solutions at different temperatures and pH values are obtained by pulsed-gradient spin-echo NMR, and their concentration dependence is analyzed. The generalized concentration dependence of globular protein self-diffusion coefficients is empirically established, and compared to the concentration dependence of diffusion coefficients of flexible polymers and rigid Brownian particles.
Nesmelova
Irina V
IV
Laboratory of Molecular Biophysics, Kazan Institute of Biochemistry and Biophysics, Kazan 420503, Russia. nesme001@tc.umn.edu
Skirda
Vladimir D
VD
Fedotov
Vladimir D
VD
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D055592:Biophysical Phenomena; D001703:Biophysics; D002417:Cattle; D004058:Diffusion; D007526:Isoelectric Point; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D013696:Temperature; D014867:Water / Q000737:chemistry
D001426:Bacterial Proteins; D009211:Myoglobin; D011506:Proteins; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; C057438:barstar protein, Bacillus amyloliquefaciens; D009113:Muramidase