Inverse relationship of skeletal muscle glycogen from wild-type and genetically modified mice to their phosphorylase a activity.
Leg muscle was biopsied and frozen for storage at -70 degrees C. from 5 wild-type mice, two knocked out acid alpha-glucosidase (GAA) gene mice, and seven glycogen synthase plus glucose muscle transporter transgenic mice. All of the wild-type mice had very little muscle glycogen (3.58 +/- 1.67 micromols glucosyl subunits per g muscle), and 52% or more of its glycogen phosphorylase activity without AMP (69% +/- 17% glycogen phosphorylase a). In contrast the GAA knockout and transgenic mice had glycogen ranging from 63 to 297 micromols glucosyl subunits per g muscle, and very little or no glycogen phosphorylase activity without 1.00 mM AMP (4.8% and less glycogen phosphorylase a). This suggests that there is an inverse relationship between mouse muscle phosphorylase a and the muscle's glycogen content.
Schliselfeld
Louis H
LH
Department of Pathology, Westchester Medical Center, Valhalla, New York, USA. schlisefeld@aol.com
Danon
Moris J
MJ
D000818:Animals; D004789:Enzyme Activation / Q000502:physiology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D051272:Glucose Transporter Type 1; D006003:Glycogen / Q000032:analysis / Q000378:metabolism*; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism; D006006:Glycogen Synthase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D025221:Phosphofructokinases / Q000378:metabolism; D010762:Phosphorylase a / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D006004:Glycogen Debranching Enzyme System; D009004:Monosaccharide Transport Proteins; D006003:Glycogen; D010762:Phosphorylase a; D006006:Glycogen Synthase; D025221:Phosphofructokinases; D005087:Glucan 1,4-alpha-Glucosidase; D005634:Fructose-Bisphosphate Aldolase