title: [Gene transfer of CD80 into a human ovarian cancer cell line and induction of cytotoxic T lymphocyte in vitro].
abstract: OBJECTIVE
  To investigate the proliferation and cytotoxicity of cytotoxic T lymphocyte (CTL) induced by ovarian cancer cells transfected with a CD80 gene containing retroviral vector.
  
  
  METHODS
  The ovarian cancer cell line TYK cells were transfected with retro-virus vector PLXSN-hCD80. After geneticin (G418) selection, the CD80 expression of the transfectants was confirmed by flow cytometry. CTL was induced by co-culture of CD80-expressing TYK cells (TYK-hCD80) and peripheral-blood mononuclear cells (PBMC) in the presence of anti-CD3 monoclonal antibody (McAb). Proliferation of PBMC was measured using 3H-Thymidine incorporation assay. The lysis activity of CTL toward tumor cells was determined using methyl thiazolyl tetrazolium (MTT) assay.
  
  
  RESULTS
  After transfection and G418 selection, the CD80 expression was proved by flow cytometry. The highest rate of CD80 expression was 84.9%. The TYK cell line expressing high CD80 was named TYK-hCD80. In the presence of anti-CD3 McAb, TYK-hCD80 significantly enhanced proliferation of PBMC than TYK cells (3H-Thymidine incorporation, (40,604 +/- 842) vs (8,000 +/- 594) cpm (P < 0.05). The lysis activity of CTL activated by TYK-hCD80 was higher than that of TYK(P < 0.05).
  
  
  CONCLUSIONS
  Ovarian cancer cells expressing CD80 can induce the production of CTLs which have high lysis activity and high proliferation rate in the presence of anti-CD3 McAb. This study may provide the experimental basis for immunogenic therapy of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: B
authors.initials: B
authors.lastname: Cui
authors.forename: B
authors.initials: B
authors.lastname: Kong
authors.forename: J
authors.initials: J
authors.lastname: Jiang
mesh_terms: D018122:B7-1 Antigen / Q000235:genetics*; D002455:Cell Division; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D012190:Retroviridae / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D018122:B7-1 Antigen