Hypotaurine protection on cell damage by singlet oxygen.
Singlet oxygen (1O2), generated by irradiating methylene blue, is toxic to melanoma cell cultures. Hypotaurine is known to scavenge efficiently singlet oxygen; the addition of hypotaurine (800 microM) to the medium during irradiation of the dye produces a greater protective effect on cells than taurine added at the same concentration. The assay of some detoxifying enzymatic activities indicate a different mechanism of protection of the two molecules: taurine induces an efficient detoxifying enzymatic action with respect to the control; hypotaurine exerts its effect greatly by specifically scavenging singlet oxygen.
Pitari
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Dipartimento di Biologia di Base ed Applicata, Università di L'Aquila, Italy.
Duprè
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Spirito
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Antonini
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Amicarelli
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D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D016923:Cell Death; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D008751:Methylene Blue / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000494:pharmacology; D026082:Singlet Oxygen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D016166:Free Radical Scavengers; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D013654:Taurine; C003949:hypotaurine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D008751:Methylene Blue