Role of cell-specific SpoIIIE assembly in polarity of DNA transfer.
SpoIIIE mediates postseptational chromosome partitioning in Bacillus subtilis, but the mechanism controlling the direction of DNA transfer remains obscure. Here, we demonstrated that SpoIIIE acts as a DNA exporter: When SpoIIIE was synthesized in the larger of the two cells necessary for sporulation, the mother cell, DNA was translocated into the smaller forespore; however, when it was synthesized in the forespore, DNA was translocated into the mother cell. Furthermore, the DNA-tracking domain of SpoIIIE inhibited SpoIIIE complex assembly in the forespore. Thus, during sporulation, chromosome partitioning is controlled by the preferential assembly of SpoIIIE in one daughter cell.
Sharp
Marc D
MD
Division of Biology, University of California, San Diego, La Jolla, CA 92093-0349, USA.
Pogliano
Kit
K
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus; D049452:Green Fluorescent Proteins