[A study on beta-lactamase activity of biofilm Escherichia coli].
OBJECTIVE
To investigate the mechanism of drug resistance of biofilm escherichia coli.


METHODS
The model of escherichia coli biofilm was established with the flat-board method. And the biofilm was confirmed by scanning electron microscopy. The beta-lactamase activities were quantitated, in escherichia coli, biofilm escherichia coli, biofilm escherichia coli induced by impenem or cefoxitin.


RESULTS
The beta-lactamase activity of biofilm escherichia coli was 2.16 times as much as that of escherichia coli planctonically, and the beta-lactamase activities of biofilm escherichia coli induceded by impenem or cefoxitin were 1.30 and 1.05 times as much as those of biofilm escherichia coli, respectively.


CONCLUSIONS
The drug resistance to antibiotics of biofilm escherichia coli was related to the production of beta-lactamase.
He
P
P
Second Affiliated Hospital, China Medical University, Shenyang 110004, China.
Li
N
N
Li
S
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms; D002440:Cefoxitin / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D001618:beta-Lactamases / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002440:Cefoxitin; D001618:beta-Lactamases