Determination of proteins by their enhancement of resonance light scattering by fuchsine acid.
Based on the measurement of the enhancement of resonance light scattering (RLS) of fuchsine acid (FSA) by proteins, a novel sensitive assay of proteins in body fluid samples has been developed. Proteins, including bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep), alpha-chymotrypsin (Chy), lysozyme (Lys), and cellulase (Cel), can bind to fuchsine acid (FSA), resulting in enhanced RLS signals at 277.0 nm. Linear relationships between the enhanced RLS intensity and the protein concentration were measured at different concentration of FSA, and the limits of detection for BSA, HSA, and Lys were found to lie in the nanogram range.
Huang
C Z
CZ
Institute of Environmental Chemistry, Southwest Normal University, Chongqing, PR China. chengzhi@swnu.edu.cn
Li
Y F
YF
Feng
P
P
Li
M
M
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002138:Calibration; D006801:Humans; D008027:Light; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014556:Urine / Q000737:chemistry
D011506:Proteins; D012394:Rosaniline Dyes