Effect of dietary beta-1,3 glucan on immune responses and disease resistance of healthy and aflatoxin B1-induced immunocompromised rohu (Labeo rohita Hamilton).
The immunostimulant beta-1,3 glucan was fed at 0.1% in feed for 7 days to healthy and aflatoxin B1 (AFB1)-induced immunocompromised fish, Labeo rohita (one of the major tropical carp species), in a 60 day trial. The effects of AFB1, glucan and their interactions on non-specific and specific immunity levels and disease resistance of fish were studied. A single intraperitoneal injection of AFB1 at 1.25 mg kg(-1) body weight) caused a significant (P < 0.05) reduction in non-specific immunity as measured through neutrophil phagocytic indices, serum bactericidal activity, and specific immunity as measured through bacterial agglutination titre against Edwardsiella tarda, as well as reduced protection against Aeromonas hydrophila challenge in comparison to control fish which were exposed neither to aflatoxin nor to glucan. Feeding of glucan to healthy fish raised the non-specific and specific immunity level and protection against bacterial infection compared with the control. Feeding of glucan to AFB1-induced immunocompromised fish for 7 days significantly raised the degree of resistance against A. hydrophila challenge and the non-specific immunity level in comparison to non-treated AFB1 exposed fish. Although feeding of glucan was able to increase specific immunity, all measured through haemagglutination titre against sheep red blood cells, and bacterial (E. tarda) agglutination titre in healthy fish in comparison to all other groups, no significant increase in specific immunity to the aflatoxin-exposed group was seen.
Sahoo
P K
PK
Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India. cifa@ori.nic.in
Mukherjee
S C
SC
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D016604:Aflatoxin B1 / Q000009:adverse effects* / Q000276:immunology; D000372:Agglutination Tests / Q000662:veterinary; D000821:Animal Feed; D000818:Animals; D003530:Cyprinidae / Q000276:immunology*; D005936:Glucans / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D016867:Immunocompromised Host; D007274:Injections, Intraperitoneal / Q000662:veterinary; D047071:beta-Glucans
D000276:Adjuvants, Immunologic; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan; D016604:Aflatoxin B1