Isolation of genes related to blood glucose-control in rat skeletal muscle.
OBJECTIVE
To isolate genes related to blood glucose-control using Sprague Dawley (SD) rat skeletal muscle.


METHODS
Differential gene expression between glucose stimulated and non-glucose stimulated SD rat skeletal muscle was obtained by the differential display (DD) method, Slot blotting hybridization and Northern blot hybridization.


RESULTS
Several new genes that are differentially expressed in glucose stimulated and non-glucose stimulated SD rat skeletal muscle were isolated. 74 were verified by slot analysis from 181 gene tags isolated. Of them, 33 were cloned and sequenced, and homologous analysis and application for GenBank Access Number were carried out. 21 expressed sequence tag (EST) representing novel genes was confirmed by Northern blot analysis. A total of 9 novel genes showed significant differential expression patterns.


CONCLUSIONS
Using improvements and modifications of the differential display technique, a labor- and cost-saving route was used to identify new genes related to blood glucose-control. We investigated differentially expressed genes at the whole body level instead of the culture cell level, to ensure experimental results closer to the normal physiological state. This technique may be valid in wide-spread application to other related research.
Zhu
G
G
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005, China.
Zuo
J
J
Fang
F
F
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005947:Glucose / Q000494:pharmacology; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D005947:Glucose