Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824.
The utilization of maltose by Clostridium acetobutylicum ATCC 824 was investigated. Glucose was used preferentially to maltose, when both substrates were present in the medium. Maltose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on maltose, but not glucose or sucrose, as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that the specificity for maltose is contained entirely within the membrane in this organism. A putative gene system for the maltose PTS was identified (from the C. acetobutylicum ATCC 824 genome sequence), encoding an enzyme II(Mal) and a maltose 6-phosphate hydrolase.
Tangney
M
M
Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, UK.
Winters
G T
GT
Mitchell
W J
WJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001692:Biological Transport; D002458:Cell Fractionation; D002474:Cell-Free System; D003013:Clostridium / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism*; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000378:metabolism; D000520:alpha-Glucosidases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D013395:Sucrose; D008320:Maltose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C107141:phosphoenolpyruvate-maltose phosphotransferase; C049150:maltose-6'-phosphate glucosidase; D000520:alpha-Glucosidases; D005947:Glucose