Molecular characterization of beta-tubulin gene from Pleurotus sajor-caju.
A beta-tubulin gene (TUB1) from the basidiomycete Pleurotus sajor-caju was sequenced. TUB1 encodes a 446-amino-acid protein. The coding region is interrupted by 9 introns, all of which had a 5'-GTRNGT... YAG-3' sequence at the boundaries. Locations of the introns in TUB1 were common between the beta-tubulin genes of other basidiomycetes, but not with animals, ascomycetes, or plants. This suggests that the introns were inserted independently into the beta-tubulin gene after these divisions had diverged.
Kim
B G
BG
Division of Applied Microbiology, National Institute of Agricultural Science and Technology, Suweon, Korea. bgkimyes@rda.go.kr
Yoo
Y B
YB
Kwon
S T
ST
Magae
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D015723:Gene Library; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020076:Pleurotus / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014404:Tubulin / Q000737:chemistry / Q000235:genetics*
D004271:DNA, Fungal; D014404:Tubulin