title: Microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese.
abstract: Phenotypic and phylogenetic studies were performed on 11 strains of a Microbacterium-like organism isolated from the surface of a smear-ripened cheese. The isolates were Gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) NaCl. Chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus Microbacterium. They contained type B1 peptidoglycans with L-lysine as the diamino acid and glycolyl acyl types; rhamnose and galactose were the cell wall sugars. The G+C content ranged from 69 to 72 mol%. The major menaquinones were MK-11 and MK-12 and the major fatty acids were anteiso C15:0 and C17:0 and iso C16:0. Phylogenetic analysis of the 16S rRNA sequences of four isolates showed that they represented a new subline in the genus Microbacterium, with Microbacterium barkeri as their nearest phylogenetic neighbour. M. barkeri showed the highest sequence similarity to the isolates; however, DNA-DNA hybridization showed that the isolates had only 38% chromosomal similarity to M. barkeri. Based on the phylogenetic and phenotypic distinctiveness of the isolates, it is proposed that they be classified as a new Microbacterium species, for which the name Microbacterium gubbeenense sp. nov. is suggested. The type strain has been deposited as LMG S-19263T (= NCIMB 30129T). The GenBank accession number for the 16S rDNA sequence of the type strain is AF263563.
authors.forename: N M
authors.initials: NM
authors.lastname: Brennan
authors.forename: R
authors.initials: R
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Goodfellow
authors.forename: A C
authors.initials: AC
authors.lastname: Ward
authors.forename: T P
authors.initials: TP
authors.lastname: Beresford
authors.forename: M
authors.initials: M
authors.lastname: Vancanneyt
authors.forename: T M
authors.initials: TM
authors.lastname: Cogan
authors.forename: P F
authors.initials: PF
authors.lastname: Fox
mesh_terms: D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002611:Cheese / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics; D005516:Food Microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S