[Antitumor effect of peri-cryosurgical immunotherapy].
OBJECTIVE
To evaluate the anti-tumor effect of combined cryosurgery and immunotherapy in mice.


METHODS
C57BL/6 mice bearing B16 melanoma were randomly divided into 3 groups: (1) peri-cryosurgical immunotherapy group, (2) cryosurgery group, (3) immunotherapy group and (4) saline group.


RESULTS
The tumor size on day 35 was 0.4 cm3 in group 1, 1.1 in group 2, 1.9 in group 3, and 3.9 in group 4 of mice. The survival period was 71, 62, 53 and 46 days, respectively. None in group 3 and 4 was cured, while 14% of mice in group 2 and 50% in group 1 were tumor tree. In tumor-free mice, no tumor cell in the primary tumor sites was found by light and electron microscopy 3 months later.


CONCLUSIONS
The combined use of cryosurgery and immunotherapy is curative in 50% of mice bearing established B16 melanoma.
Zhang
B
B
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Zhang
S
S
Qu
P
P
D000818:Animals; D003131:Combined Modality Therapy; D003452:Cryosurgery; D007167:Immunotherapy; D008546:Melanoma, Experimental / Q000401:mortality / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL