title: Analysis of a growth-promoting factor for Babesia caballi cultivation.
abstract: Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO2 in air. Growth of B. caballi was dependent of the suitable concentration (10-20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.
authors.affiliation: Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ikadai
authors.forename: M D
authors.initials: MD
authors.lastname: Martin
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: N
authors.initials: N
authors.lastname: Kudo
authors.forename: T
authors.initials: T
authors.lastname: Oyamada
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D001403:Babesia / Q000254:growth & development*; D016895:Culture Media, Serum-Free; D006133:Growth Substances
chemical_list: D016895:Culture Media, Serum-Free; D006133:Growth Substances