The mitochondrial permeability transition and taurine.
Perturbed cellular calcium homeostasis has been implicated in both apoptosis and necrosis, but the role of altered mitochondrial calcium handling in the cell death process is unclear. Recently we found that taurine, a naturally occurring amino acid potentiates Ca2+ sequestration by rat liver mitochondria. These data, which accounted for the taurine antagonism on Ca2+ release induced by the neurotoxins 1-methyl-4-phenylpyridinium plus 6-hydroxy dopamine previously reported, prompted us to investigate the effects of taurine on the permeability transition (PT) induced experimentally by high Ca2+ plus phosphate concentrations. The parameters used to measure the PT were, mitochondrial swelling, cytochrome c release and membrane potential changes. The results showed that, whereas taurine failed to reverse changes of these parameters, cyclosporin A completely reversed them. Even though these results exclude a role in PT regulation under such gross insult conditions, they cannot exclude an important role for taurine in controlling pore-opening under milder more physiological PT-inducing conditions.
Palmi
M
M
Istituto di Scienze Farmacologiche, Università di Siena, Italy.
Youmbi
G T
GT
Sgaragli
G
G
Meini
A
A
Benocci
A
A
Fusi
F
F
Frosini
M
M
Della Corte
L
L
Davey
G
G
Tipton
K F
KF
D000818:Animals; D002118:Calcium / Q000378:metabolism; D007425:Intracellular Membranes / Q000502:physiology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010539:Permeability; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000378:metabolism* / Q000494:pharmacology
D010710:Phosphates; D013654:Taurine; D002118:Calcium