title: Thermally responsive polymer-grafted surfaces facilitate patterned cell seeding and co-culture.
abstract: Tissue engineering constructs that effectively duplicate natural tissue function must also maintain tissue architectural and organization features, particularly the integration of multiple cell types preserving distinct, integrated phenotypes. Cell-cell communication and biochemical cross-talk have been shown to be essential for the maintenance of differentiated cell functions in tissues and organs. Current limitations of cell-culture hinder progress in understanding the features and dynamics of heterotypic cell communication pathways critical to developing more sophisticated or effective tissue-engineered devices. We describe a method to conveniently electron-beam pattern cell culture surfaces with thermo-responsive polymer chemistry that exploits changes in cell-polymer adhesive interactions over a temperature window amenable for high-throughput cell culture. Cells seeded on these patterned surfaces at 20 degrees C adhere only to surface areas lacking thermo-responsive grafting chemistry; grafted domains at 20 degrees C are hydrophilic and non-cell adhesive. The culture temperature is then increased to 37 degrees C collapsing the hydrated grafted chemistry. A second cell type is added to the culture and adheres only to these exposed relatively hydrophobic grafted patterns. Both cell types can then be effectively co-cultured at 37 degrees C under multiple conditions. Long-term cell pattern fidelity and differentiated cell functions characteristic of each co-planar cell type are observed. This method is simple and has few limitations, compared with other existing co-culture methods.
authors.affiliation: Institute of Biomedical Engineering, Tokyo Women's Medical University, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Chie
authors.initials: C
authors.lastname: Konno
authors.forename: Mika
authors.initials: M
authors.lastname: Utsumi
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018920:Coculture Techniques; D008297:Male; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D013696:Temperature
chemical_list: D011108:Polymers