In vivo gene delivery via portal vein and bile duct to individual lobes of the rat liver using a polylysine-based nonviral DNA vector in combination with chloroquine.
The objective of this study was to evaluate a bifunctional synthetic peptide as a DNA vector for regional gene delivery to the rat liver by the portal vein and bile duct routes. The 31-amino-acid peptide (polylysine-molossin) comprises an amino-terminal chain of 16 lysines for electrostatic binding of DNA, and the 15 amino acid integrin-binding domain of the venom of the American pit viper, Crotalus molossus molossus. Initial in vitro evaluation demonstrated that polylysine-molossin/DNA complexes were much smaller (approximately 50-100nm versus 500-1300nm), more positively charged, and more stable in isotonic dextrose in comparisons with salt-containing solutions. However, polylysine-molossin/DNA complexes in any solution other than complete culture medium were ineffective for gene delivery in vitro. Vector localization studies demonstrated that both the portal vein and bile duct routes provided excellent access of polylysine-molossin/DNA complexes to the liver. However, complexes delivered by the portal vein were rapidly lost (<15 min) following re-establishment of the portal circulation, whereas complexes delivered by the bile duct persisted much longer. Polylysine-molossin/DNA complexes in various isotonic solutions were delivered to the right lateral lobes either by perfusion through a branch of the portal vein or by infusion into appropriate branches of the bile duct. Two or three hours before gene delivery, rats were given a single injection of chloroquine. We report that the polylysine-molossin vector is much more effective (>10-fold) when delivered by the bile duct route with all isotonic solutions evaluated, and that polylysine-molossin/DNA complexes in isotonic dextrose are much more effective (>10-fold) than complexes in salt-containing solutions.
Zhang
X
X
Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St. Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ, UK.
Collins
L
L
Sawyer
G J
GJ
Dong
X
X
Qiu
Y
Y
Fabre
J W
JW
D000818:Animals; D001652:Bile Ducts; D001665:Binding Sites; D002738:Chloroquine / Q000008:administration & dosage / Q000494:pharmacology*; D003435:Crotalid Venoms; D017839:Crotalus; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008099:Liver; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides; D011107:Polylysine; D011169:Portal Vein; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D003435:Crotalid Venoms; D016023:Integrins; D009842:Oligopeptides; C418682:molossin peptide; D011107:Polylysine; D002738:Chloroquine