[Enumeration of active cells in bacterial plankton of the Rybinsk reservoir with 5-cyano-2,3-ditolyltetrasolium: comparison with other methods].
The enumeration of actively respiring bacterial cells in different biotopes of the littoral zone of the Rybinsk Reservoir during the spring period of ice thaw using the fluorescent dye 5-cyano-2,3-ditolyl tetrazolium chloride showed that bacterial communities growing on the bottom surface of the ice cover and in water overgrown by higher aquatic plants were most active. The number of active cells among individual bacterial cells averaged 20% and reached about 40% among aggregated and filamentous bacterial cells. The results of the count of active bacteria by this method were compared with those obtained by other methods.
Kosolapov
D B
DB
Papanin Institute of the Biology of Inland Waters, Russian Academy of Sciences, Borok, Nekouzskii raion, Yaroslavl Oblast, 152742 Russia.
Kopylov
A I
AI
D000818:Animals; D001419:Bacteria / Q000166:cytology*; D005456:Fluorescent Dyes; D005618:Fresh Water; D008856:Microscopy, Fluorescence; D010933:Plankton / Q000382:microbiology*; D012426:Russia; D013778:Tetrazolium Salts
D005456:Fluorescent Dyes; D013778:Tetrazolium Salts; C075453:5-cyano-2,3-ditolyltetrazolium
Opredelenie soderzhaniia aktivnykh kletok v bakterioplanktone Rybinskogo vodokhranilishcha s pomoshch'iu 5-tsiano-2,3-ditoliltetrazoliia: sravnenie s drugimi metodami.