[Influence of interleukin-1 and dexamethasone on prostaglandin production of condylar chondrocytes].
OBJECTIVE
To study the effect of recombinant human interleukin-1(rhIL-1) and dexamethasone on the amount of 6-keto-prostaglandin F1 alpha produced by condylar chondrocytes.


METHODS
The concentration of 6-keto-prostaglandin F1 alpha of the chondrocytes supernate was detected respectively at 4, 8, 12, 24, and 72 hours, using the method of radioimmunoassay, after being stimulated by rhIL-1 and dexamethasone for four hours.


RESULTS
In normal control group, the concentration of 6-keto-prostaglandin F1 alpha was 1,516.49 ng/L, 1,513.22 ng/L, 1,506.76 ng/L, 1,526.79 ng/L and 2,114.36 ng/L at 4, 8, 12, 24 and 72 hours respectively. In rhIL-1 group, the concentration of 6-keto-prostaglandin F1 alpha was 1,664.32 ng/L at four hours, which was sharply higher than that of the control group(P < 0.01); the concentration of 6-keto-prostaglandin F1 alpha was 1,146.11 ng/L, 949.24 ng/L, 1,392.33 ng/L and 1,481.98 ng/L at 8, 12, 24 and 72 hours respectively, which were sharply lower than that of the control group(P < 0.01). After the condylar chondrocytes were stimulated with dexamethasone for 4 hours, the concentration of 6-keto-prostaglandin F1 alpha decreased more markedly than that of the control group(P < 0.01) throughout the observation period, both in the presence and absence of rhIL-1.


CONCLUSIONS
rhIL-1 could enhance the production of 6-keto-prostaglandin F1 alpha synthesized by condylar chondrocytes. Dexamethasone could inhibit the activity of rhL-1 and the production of 6-keto-prostaglandin F1 alpha synthesized by condylar chondrocytes.
Yao
X
X
School of Stomatology, Beijing Medical University, Beijing 100081.
Ma
X
X
Zhang
Z
Z
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D019902:Chondrocytes / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008335:Mandibular Condyle / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology
D007375:Interleukin-1; D011994:Recombinant Proteins; D015121:6-Ketoprostaglandin F1 alpha; D003907:Dexamethasone