[Autotrophic synthesis of polyalkanoates by Alcaligenes eutrophus in presence of carbon monoxide].
The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol% CO did not inhibit the key enzymes of PHA synthesis--beta-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt% PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol% beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
Volova
T G
TG
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia.
Kalacheva
G S
GS
Altukhova
O V
OV
D000473:Alkanes / Q000378:metabolism*; D002248:Carbon Monoxide / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003470:Culture Media; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D006880:Hydroxy Acids / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D013058:Mass Spectrometry; D009584:Nitrogen / Q000172:deficiency; D011091:Polyesters / Q000378:metabolism*
D000473:Alkanes; D003470:Culture Media; D005227:Fatty Acids; D006880:Hydroxy Acids; D008055:Lipids; D011091:Polyesters; D002248:Carbon Monoxide; D009584:Nitrogen
Avtotropnyĭ sintez polioksialkanoatov bakteriiami Alcaligenes eutrophus v prisutstvii monookisi ugleroda.