title: Serologic survey for hantavirus infections among wild animals in rural areas of São Paulo State, Brazil.
abstract: A serosurvey was conducted in wild animals captured close to two areas where hantavirus cardiopulmonary syndrome (HCPS) occurred in São Paulo State, Brazil. Serum samples from a total of 43 mammals were tested for antibodies reactive with Sin Nombre (SN) hantavirus using a strip immunoblot assay. RNAs from the blood clots of the positive samples were submitted to reverse transcriptase-polymerase chain reaction (RT-PCR). Two rodents of the genus Oligoryzomys were positive for hantavirus antibodies. These animals were captured in the Iguape region and represented 16.7% (2/12) of the sera from rodents and 100.0% (2/2) of the Oligoryzomys captured in that area. RT-PCR failed to amplify any viral cDNA. These results are in agreement with other data that suggest that members of this genus are important reservoirs of hantaviruses in Brazil.
authors.affiliation: Department of Public Health Practices, School of Public Health, University of São Paulo, São Paulo, SP, 01246-904, Brazil. nicolina@usp.br
authors.forename: N S
authors.initials: NS
authors.lastname: Romano-Lieber
authors.forename: J
authors.initials: J
authors.lastname: Yee
authors.forename: B
authors.initials: B
authors.lastname: Hjelle
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000821:virology*; D000914:Antibodies, Viral / Q000302:isolation & purification; D001938:Brazil; D002335:Carnivora / Q000821:virology; D004197:Disease Reservoirs; D006476:Orthohantavirus / Q000276:immunology / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D018804:Hantavirus Pulmonary Syndrome / Q000821:virology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012377:Rodentia / Q000821:virology
chemical_list: D000914:Antibodies, Viral