Association of MR1 protein, an MHC class I-related molecule, with beta(2)-microglobulin.
MR1 is a major histocompatibility complex (MHC) class I-related gene conserved among mammals, and its predicted amino acid sequence is relatively closer to the classical MHC class I molecules among several divergent class I molecules. However, as its molecular nature and function have not yet been clarified, we set out in this study to establish transfected P388 murine cell lines that stably produce a large number of MR1 proteins and conducted analyses to investigate the molecular nature of MR1. Immunoprecipitation and Western blot analyses with specific antisera revealed that the MR1 protein can associate with beta(2)-microglobulin, suggesting its molecular form of a typical class I heterodimer composed of a heavy and a light chain (beta(2)-microglobulin), like the classical MHC class I molecules.
Yamaguchi
Hisateru
H
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, 470-1192, Japan.
Hashimoto
Keiichiro
K
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D019281:Dimerization; D006031:Glycosylation; D006596:Hexosaminidases / Q000737:chemistry; D015395:Histocompatibility Antigens Class I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007941:Leukemia P388 / Q000378:metabolism*; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009844:Oligosaccharides / Q000737:chemistry; D011233:Precipitin Tests; D014162:Transfection; D001613:beta 2-Microglobulin / Q000378:metabolism*
D015395:Histocompatibility Antigens Class I; D015778:Minor Histocompatibility Antigens; C490628:Mr1 protein, mouse; D009844:Oligosaccharides; D001613:beta 2-Microglobulin; D006596:Hexosaminidases