Sustained expression of the novel EBV-induced zinc finger gene, ZNFEB, is critical for the transition of B lymphocyte activation to oncogenic growth transformation.
EBV is a human tumor virus that infects and establishes latency in the majority of humans worldwide. In vitro, EBV growth transforms primary B lymphocytes into lymphoblastoid cell lines with high efficiency. We have used cDNA subtraction cloning to identify cellular target genes required for growth transformation and identified a new C(2)H(2) (Krüppel-type) zinc finger gene, ZNF(EB), that is trans-activated early following EBV infection. In this study, we characterize ZNF(EB), including its intronless locus, and human and mouse protein variants. The gene is transiently expressed during normal lymphocyte activation, and its expression is sustained in EBV-positive but not EBV-negative B cell lines. There is limited expression in nonhemopoietic tissues. Its critical role in the growth transformation of B lineage cells is indicated by the abrogation of transformation with antisense strategies. ZNF(EB) maps to chromosome 18q12, a region with mutations in numerous, predominantly hemopoietic malignancies.
Tune
Cathryn E
CE
Division of Infection, Immunity, Injury, and Repair, The Hospital for Sick Children, Toronto, Ontario, Canada.
Pilon
Marc
M
Saiki
Yuriko
Y
Dosch
H-Michael
HM
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000302:isolation & purification; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D002472:Cell Transformation, Viral / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D015723:Gene Library; D018922:HL-60 Cells; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008213:Lymphocyte Activation / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics; D017422:Sequence Analysis, DNA; D020298:U937 Cells; D016335:Zinc Fingers / Q000235:genetics* / Q000276:immunology
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; C444979:ZNF271P protein, human; C499168:ZNFEB protein, mouse