[Detection of HPV in human esophageal cancer in high-incidence area and its correlation with p53 expression].
OBJECTIVE
To investigate the association of HPV with the development of esophageal cancer (EC) in a high-incidence area of EC and to elucidate its correlation with p53 overexpression.


METHODS
Thirty EC specimens were collected from Anyang, Henan. Four pairs of primers were designed to perform in situ hybridization (ISH) and in situ PCR(ISPCR). Immunohistochemical staining was used to detect p53.


RESULTS
HPV L1, HPV-16-E6 and HPV-16-E7 was detected in 10.0%, 60.0% and 63.3% of the EC samples, respectively. The detection rate of HPV-18-E6 was low(6.7%) and no EBV was detected. Overexpression of p53 was identified in 73.3% EC. With ISH or ISPCR, HPV-16-E6 was positive in 53.3% of EC.


CONCLUSIONS
The low detection rate of HPV L1 and high detection rate of HPV-16-E6 and E7 genes suggest that HPV may be partially lost when integrating into tumor cell genome, while E6 and E7 genes are intact. The results support a role of HPV-16 in the pathogenesis of EC in high incidence area. Although p53 mutation takes an important part in tumor pathogenesis, it is not consistent with the HPV existence in the EC cells.
Lu
Z
Z
Department of Genetics, Beijing Institute for Cancer Research, Tumor Hospital, Beijing University, Beijing 100034, China.
Chen
K
K
Guo
M
M
D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D006801:Humans; D017403:In Situ Hybridization; D009856:Oncogene Proteins, Viral / Q000032:analysis* / Q000235:genetics; D027383:Papillomaviridae / Q000737:chemistry; D050725:Papillomavirus E7 Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; C059731:oncogene protein E7, Human papillomavirus type 16