Inter-receptor communication through arrays of bacterial chemoreceptors.
The sensing mechanisms of chemotactic bacteria allow them to respond sensitively to stimuli. Escherichia coli, for example, respond to changes in chemoattractant concentration of less than 10% over a range spanning six orders of magnitude. Sensitivity over this range depends on a nonlinear relationship between ligand concentration and output response. At low ligand concentrations, substantial amplification of the chemotactic signal is required; however, the mechanism responsible for this amplification remains unclear. Here we demonstrate that inter-receptor communication within a lattice acts to amplify and integrate sensory information. Synthetic multivalent ligands that interact through the low-abundance, galactose-sensing receptor Trg stabilize large clusters of chemoreceptors and markedly enhance signal output from these enforced clusters. On treatment with multivalent ligands, the response to the attractant serine is amplified by at least 100-fold. This amplification requires a full complement of chemoreceptors; deletion of the aspartate (Tar) or dipeptide (Tap) receptors diminishes the amplification of the serine response. These results demonstrate that the entire array is involved in sensing. This mode of information exchange has general implications for the processing of signals by cellular receptors.
Gestwicki
Jason E
JE
Department of Chemistry, University of Wisconsin-Madison, 53706, USA.
Kiessling
Laura L
LL
D001426:Bacterial Proteins / Q000378:metabolism; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins; D017459:Receptors, Amino Acid / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*
D001426:Bacterial Proteins; D002630:Chemotactic Factors; D029968:Escherichia coli Proteins; D008024:Ligands; D008565:Membrane Proteins; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; C067124:Tap protein, E coli; C041476:Trg protein, E coli; C031959:aspartic acid receptor; D012694:Serine