title: Determination of hydrogen peroxide by iron(III) complex of thiacalix[4]arenetetrasulfonate on a modified ion-exchanger with peroxidase-like catalytic activity.
abstract: An iron(III) complex of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger (Fe3+-TCAS(A-500)) has shown high peroxidase-like activity at pH 5 - 6 for the reaction of quinoid-dye formation between 3-methyl-2-benzothiazolinone hydrazone and N-(3-sulfopropyl)aniline in the presence of hydrogen peroxide. Utilizing the peroxidase-like activity of Fe3+-TCAS(A-500) for this reaction, a method using Fe3+-TCAS(A-500) was applied for the spectrophotometric determination of hydrogen peroxide. The calibration curve by the method using Fe3+-TCAS(A-500) was linear over the range from 1 to 10 microg of hydrogen peroxide in a 1 ml sample solution. The apparent molar absorptivity for hydrogen peroxide was 2.4 x 10(4) l mol(-1) cm(-1). which was about 80% of that by peroxidase under the same conditions. This determination method of hydrogen peroxide using Fe3+-TCAS(A-500) was applied for the determination of glucose in diluted normal and abnormal control serum I and II.
authors.affiliation: Department of Biological Chemistry, Faculty of Science, Okayama University of Science, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Odo
authors.forename: Y
authors.initials: Y
authors.lastname: Inomata
authors.forename: H
authors.initials: H
authors.lastname: Takeya
authors.forename: S
authors.initials: S
authors.lastname: Miyanari
authors.forename: H
authors.initials: H
authors.lastname: Kumagai
mesh_terms: D002138:Calibration; D002384:Catalysis; D005290:Ferric Compounds / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D007475:Ion Exchange Resins / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D005290:Ferric Compounds; D007475:Ion Exchange Resins; D010636:Phenols; C446239:thiacalix(4)arenetetrasulfonate; D006861:Hydrogen Peroxide; D010544:Peroxidases