title: Primary prostate stromal cells modulate the morphology and migration of primary prostate epithelial cells in type 1 collagen gels.
abstract: The effects of human primary prostatic stromal cells on the migration and morphogenesis of human prostatic epithelial cells, derived from tumor or benign prostatic hyperplasia tissue, were studied using a three-dimensional coculture system. Epithelial cells from tumor or benign tissue migrated efficiently into collagen gels populated with stromal cells from benign tissue. Only epithelial cells from benign prostate formed acinus-like structures that exhibited differentiated prostatic function and strong expression of membrane-associated E-cadherin. In gels populated by stromal cells from tumor tissue, migration of primary prostatic epithelial cells did not occur. In the absence of stromal cells, primary epithelial cells were unable to proliferate. This three-dimensional culture system allows closely controlled manipulation and analysis in vitro of interactions between prostatic epithelial and stromal cells.
authors.affiliation: YCR Cancer Research Unit, Department of Biology, University of York, York YO10 5YW, United Kingdom. jh42@york.ac.uk
authors.forename: June A
authors.initials: JA
authors.lastname: Hall
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Maitland
authors.forename: Mike
authors.initials: M
authors.lastname: Stower
authors.forename: Shona H
authors.initials: SH
authors.lastname: Lang
mesh_terms: D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D018920:Coculture Techniques; D024042:Collagen Type I; D004847:Epithelial Cells / Q000473:pathology; D005782:Gels; D006801:Humans; D008297:Male; D010641:Phenotype; D011470:Prostatic Hyperplasia / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D024042:Collagen Type I; D005782:Gels