title: Semi-quantitative RT-PCR analysis of environmental influence on P450scc and PNMT mRNA expression in rat adrenal glands.
abstract: Environmental influence on brain function, particularly spatial learning and memory, has been extensively investigated, but little is known about the influence of environmental conditions on the functions of peripheral organs. In the present study, the effects of different housing conditions on the steady-state levels of mRNAs encoding cholesterol side-chain cleavage enzyme (cytochrome P450scc) and phenylethanolamine N-methyltransferase (PNMT) in adrenal glands was examined to investigate the environmental influence on both adrenocortical and adrenomedullary functions. Behavioral changes of the animals housed in different conditions were first examined to assess the relevance of environmental manipulation used. In consistent with previous findings, housing of the animals in enriched conditions resulted in the significant reduction of spontaneous motor activity (locomotor activity and rearing) in comparison with housing in isolated conditions, thus indicating the relevance of housing conditions used in this work for investigating the environmental influence on adrenal function. Then, the effects of these housing conditions on P450scc and PNMT mRNA levels in adrenal glands were examined using semi-quantitative RT-PCR method. In comparison with the isolated group, the enriched group showed significantly higher levels of P450scc mRNA. In contrast, PNMT mRNA levels in the enriched group were significantly lower than those in the isolated group. These results propose the possibility that the environmental conditions may cause differential alterations in adrenocortical and adrenomedullary functions, although their possible association with behavioral changes still remains to be elucidated.
authors.affiliation: Department of Pharmacology, Tokushima University School of Medicine, Kuramoto, Japan. km@basic.med.tokushima-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: A
authors.initials: A
authors.lastname: Kuwada
authors.forename: A
authors.initials: A
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Sakata
authors.forename: H
authors.initials: H
authors.lastname: Sei
authors.forename: Y
authors.initials: Y
authors.lastname: Morita
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000822:Animal Husbandry; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004777:Environment; D008297:Male; D009043:Motor Activity / Q000502:physiology; D010625:Phenylethanolamine N-Methyltransferase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D002786:Cholesterol Side-Chain Cleavage Enzyme; D010625:Phenylethanolamine N-Methyltransferase