title: Marine sponge collagen: isolation, characterization and effects on the skin parameters surface-pH, moisture and sebum.
abstract: A previously described isolation procedure for collagen of the marine sponge Chondrosia reniformis Nardo was modified for scaling-up reasons yielding 30% of collagen (freeze-dried collagen in relation to freeze-dried sponge). Light microscope observations showed fibrous structures. Transmission electron microscopy studies proved the collagenous nature of this material: high magnifications showed the typical periodic banding-pattern of collagen fibres. However, the results of the amino acid analysis differed from most publications, presumably due to impurities that still were present. In agreement with earlier studies, sponge collagen was insoluble in dilute acid mediums and all solvents investigated. Dispersion of collagen was facilitated when dilute basic mediums were employed. The acid-base properties of the material were investigated by titration. Furthermore, a sponge extract was incorporated in two different formulations and compared with their extract-free analogues and a commercially available collagen containing product with respect to their effects on biophysical skin parameters. None of the preparations had a noticeable influence on the physiological skin surface pH. Skin hydration increased only slightly. However, all tested formulations showed a significant increase of lipids measured by sebumetry.
authors.affiliation: Institute of Pharmaceutical Technology, Johann Wolfgang Goethe 1-1 University, Biocenter, Marie-Curie-Strasse 9, 60439 Frankfurt am Main, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Swatschek
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schatton
authors.forename: Josef
authors.initials: J
authors.lastname: Kellermann
authors.forename: Werner E G
authors.initials: WE
authors.lastname: Müller
authors.forename: Jörg
authors.initials: J
authors.lastname: Kreuter
mesh_terms: D000328:Adult; D000818:Animals; D002626:Chemistry, Pharmaceutical; D003094:Collagen / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D006801:Humans; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D011161:Porifera / Q000737:chemistry / Q000378:metabolism; D012629:Sebum / Q000187:drug effects* / Q000378:metabolism; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003094:Collagen