[Influence of newly synthesized sesquiterpenes--analogs of taxol on multiplication of herpes simplex virus (HSV-1MC) and retrovirus (Mo-MSV)].
The study comprised newly synthesized sesquiterpenoid analogs of taxol. The synthesis of the compounds was performed at the Institute of Organic Chemistry, Polish Academy of Sciences. Cytotoxicity of the compound was assessed using formazan method. In in vitro studies the cell cultures were infected with HSV-1MC. The tested compounds were added in different concentrations to the cell culture after viral infection. Titer of the virus was expressed in TCID50/ml at particular stages of the experiments. In in vivo experiments NMRI mice were infected intramuscularly with a Moloney murine sarcoma virus (Mo-MSV). Tested compounds were administered to the mice intravenously on the day of virus inoculation. In Mo-MSV-infected mice dynamics of tumor progression and regression was assessed, as well as a mean time interval of tumor disappearance. Among the compounds tested: isovellerol-13-N-benzoyl-(2'R,3'S)-3'-phenylisoserinate, 5-deoxy-lactarolid B 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] and isolactarorufin 8-epi-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] showed significant antiviral activity in in vitro experiments. In in vivo experiments only lactarorufin A 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] significantly inhibited the development of tumors and shortened the time of their total regression in the course of Mo-MSV infection.
Kobus
M
M
Katedra i Zakład Mikrobiologii Lekarskiej, Centrum Biostruktury, Akademia Medyczna w Warszawie.
Krawczyk
E
E
Kopczacki
P
P
Daniewski
W
W
Łuczak
M
M
D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D002478:Cells, Cultured; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008980:Moloney murine sarcoma virus / Q000187:drug effects* / Q000502:physiology; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D012192:Retroviridae Infections / Q000188:drug therapy; D012513:Sarcoma, Experimental / Q000188:drug therapy; D012717:Sesquiterpenes / Q000138:chemical synthesis / Q000494:pharmacology*; D014412:Tumor Virus Infections / Q000188:drug therapy; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D012717:Sesquiterpenes; D017239:Paclitaxel
Wpływ nowo zsyntetyzowanych seskwiterpenowych analogów taksolu na namnazanie wirusa opryszczki (HSV-1 MC) oraz retrowirusa (Mo-MSV).