Molecular characterization of a Thinopyrum intermedium group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus.
The wheat--Thinopyrum intermedium addition lines Z1 and Z2 carry 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes (2Ai-2) conferring resistance to barley yellow dwarf virus (BYDV). GISH results using the genomic DNA of Pseudoroegneria strigosa (S genome) as the probe indicated that the 2Ai-2 chromosome in Z1 and Z2 is an S-J intercalary translocation. Most of the 2Ai-2 chromosome belongs to the S genome, except for about one third in the middle region of the long arm that belongs to the J genome. The results of detailed RFLP analyses confirmed that the 2Ai-2 chromosome is extensively homoeologous to wheat group 2 chromosomes. Some new RFLP markers specific to the 2Ai-2 chromosome were identified. A RAPD marker, OP-R16(340), specific to the 2Ai-2 chromosome, was screened. We converted the RAPD marker into a sequence-characterized amplified region (SCAR) marker (designated SC-R16). The study establishes the basis for selecting translocation lines with small segments of the 2Ai-2 chromosome and localizing the BYDV resistance gene when introgressed into a wheat background.
Zhang
Z Y
ZY
Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Beijing, China. zengyanzhang@yahoo.com
Xin
Z Y
ZY
Larkin
P J
PJ
D002678:Chimera / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D007113:Immunity, Innate / Q000235:genetics*; D017793:Luteovirus; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics*
D005819:Genetic Markers