title: DNA fragmentation induced in K562 cells by nitrogen ions.
abstract: This study was aimed at investigating the radiation induced DNA fragmentation pattern as a function of cellular differentiation and radiation quality. DNA double strand breaks (DSB) induced by gamma-rays were analyzed in K562 human proerythroblasts before (AP cells) and after (D cells) differentiation induction while DNA DSB induced by 125 keV/micrometers N-ions have been studied in AP cells. Pulsed-Field Gel Electrophoresis (PFGE) of cellular DNA was used to determine the DSB yield by analysis of the Fraction of Activity Released (FAR) and of the fragmentation pattern in a specific size range (5.7-0.225 Mbp). The results so far obtained show that the DSB induction by gamma-rays is different if evaluated with the FAR or with the fragmentation analysis. The DSB yield obtained with the former method is about 1.4 times higher in AP respect to D cells while the latter method indicates that more fragments are produced in D cells. Comparison between gamma-rays and N-ions in AP cells shows that no significant differences are detected by the FAR analysis; otherwise fragmentation analysis demonstrates a higher effectiveness of nitrogen ions.
authors.affiliation: Physics Laboratory, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Belli
authors.forename: V
authors.initials: V
authors.lastname: Dini
authors.forename: G
authors.initials: G
authors.lastname: Esposito
authors.forename: P
authors.initials: P
authors.lastname: Micera
authors.forename: O
authors.initials: O
authors.lastname: Sapora
authors.forename: C
authors.initials: C
authors.lastname: Signoretti
authors.forename: G
authors.initials: G
authors.lastname: Simone
authors.forename: B
authors.initials: B
authors.lastname: Stenerlow
authors.forename: M A
authors.initials: MA
authors.lastname: Tabocchini
mesh_terms: D002454:Cell Differentiation / Q000528:radiation effects*; D017357:Cyclotrons; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D007477:Ions; D020014:K562 Cells / Q000528:radiation effects*; D009584:Nitrogen
chemical_list: D007477:Ions; D009584:Nitrogen