[Detection of transfusion transmitted virus infection and genotypes in pregnant women].
OBJECTIVE
To clarify the frequency, the routes of transmission from mother to infant, the correlation factors and distribution of genotypes of transfusion transmitted virus (TTV) infection.


METHODS
Nested-polymerase chain reaction (n-PCR) was performed to detect TTV DNA and genotypes in serums and breast milks from 160 cases of pregnant women.


RESULTS
TTV DNA in serum and breast milk was detected in 64 (40.0%) and 60 (37.5%) of 160 cases of pregnant women respectively. The positive rates of TTV DNA from HBV markers (+) and normal groups were 50.0%, 43.1% and 13.6%, 22.7% respectively in serums and breast milk. There were significant difference between the two groups (P < 0.05). The TTV genotypes were type I in all 124 samples of TTV DNA detected.


CONCLUSIONS
TTV infection is common in the pregnant women and a high prevalence of TTV infection in pregnant women with positive HBV markers has been demonstrated. TTV infection is correlated with HBV. TTV is probably transmitted from mother to their children by breast feeding. All of TTV genotypes detected in pregnant women is genotype I.
Zhang
Z
Z
Molecular Biology Lab, 177th Hospital of The Peoples Liberation Army, Guangzhou 510317, China.
Zhang
Z
Z
D000328:Adult; D004266:DNA Virus Infections / Q000235:genetics / Q000635:transmission* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008895:Milk, Human / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D022783:Torque teno virus; D065227:Transfusion Reaction
D004279:DNA, Viral