title: Evaluation of the Bartels Legionella Urinary Antigen enzyme immunoassay.
abstract: The Bartels Legionella Urinary Antigen enzyme immunoassay (Intracel, USA) is intended for the presumptive diagnosis of past or current Legionnaires' disease by qualitative detection of Legionella pneumophila serogroup 1 antigen in human urine. This test was evaluated using single urine samples collected from 349 patients with lower respiratory tract infection of known aetiology. Specificity was estimated as 100% (181 samples, 95% CI: 98%-100%); sensitivity for Legionella pneumophila serogroup 1 was 98.8% (167 samples, 95% CI: 95.7%-99.9%). Assessing assay results using a Visual Interpretation Card provided by the manufacturer in place of a photometer gave rise to one false-positive result among the 78 control samples examined. Providing the endpoint of this assay is determined photometrically, the Bartels Legionella Urinary Antigen enzyme immunoassay appears to be a highly specific and sensitive kit for the diagnosis of infection caused by Legionella pneumophila serogroup 1.
authors.affiliation: Respiratory and Systemic Infection Laboratory, PHLS Central Public Health Laboratory, London, United Kingdom. tharrison@phls.nhs.uk
authors.forename: T G
authors.initials: TG
authors.lastname: Harrison
authors.forename: N
authors.initials: N
authors.lastname: Doshi
mesh_terms: D000942:Antigens, Bacterial / Q000652:urine*; D016001:Confidence Intervals; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D016952:Legionella pneumophila / Q000276:immunology* / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000175:diagnosis* / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic