title: Oligoclonal T-cells in blood and target tissues of patients with anti-Hu syndrome.
abstract: T-cell clones of unknown significance (TCUS), assessed by monoclonal or oligoclonal T-cell patterns in PCR-DGGE, were detected in blood of 7/9 patients with anti-Hu syndrome. Clonal patterns were also detected in 2/2 neoplastic lymph nodes, and in 2/2 inflamed dorsal root ganglia from three patients. Only some T-cell clones found in target tissues were also detected in blood or non-target tissues, and likely corresponded to TCUS. In one patient, an identical T-cell clone was found in both neoplastic lymph node tissue and dorsal root ganglia, but not in blood. Dorsal root-infiltrating lymphocytes were cytotoxic CD8(+) TIA-1(+) T-cells. They were often found in close contact to sensory neurons, most of which expressed MHC-1. Taken together, these data support a direct effector role of cytotoxic CD8(+) T-cells, the same clones being likely operative in sensory neuron damage and immune-mediated tumor growth control.
authors.affiliation: INSERM E0011, "Système neuromusculaire et inflammation", Faculté de Médecine Paris XII, 94010, Créteil, France.
authors.forename: A
authors.initials: A
authors.lastname: Plonquet
authors.forename: R K
authors.initials: RK
authors.lastname: Gherardi
authors.forename: A
authors.initials: A
authors.lastname: Créange
authors.forename: J C
authors.initials: JC
authors.lastname: Antoine
authors.forename: B
authors.initials: B
authors.lastname: Benyahia
authors.forename: W
authors.initials: W
authors.lastname: Grisold
authors.forename: M
authors.initials: M
authors.lastname: Drlicek
authors.forename: P
authors.initials: P
authors.lastname: Dreyfus
authors.forename: J
authors.initials: J
authors.lastname: Honnorat
authors.forename: C
authors.initials: C
authors.lastname: Khouatra
authors.forename: H
authors.initials: H
authors.lastname: Rouard
authors.forename: F J
authors.initials: FJ
authors.lastname: Authier
authors.forename: J P
authors.initials: JP
authors.lastname: Farcet
authors.forename: J Y
authors.initials: JY
authors.lastname: Delattre
authors.forename: M H
authors.initials: MH
authors.lastname: Delfau-Larue
mesh_terms: D000328:Adult; D000368:Aged; D002999:Clone Cells; D051959:ELAV Proteins; D005260:Female; D005727:Ganglia, Spinal / Q000473:pathology; D015329:Gene Rearrangement, T-Lymphocyte / Q000276:immunology; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009443:Neuritis / Q000276:immunology; D020364:Paraneoplastic Polyneuropathy / Q000276:immunology* / Q000473:pathology; D016601:RNA-Binding Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology*
chemical_list: D051959:ELAV Proteins; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins