Ibuprofen interference in the determination of 3-phenoxybenzoic acid in urine.
Pyrethroid insecticides are widely used in agriculture and private households. Analysis of urine for pyrethroid metabolites is one way to detect human exposure to these insecticides and is carried out regularly as part of the Occupational and Environmental Medicine Monitoring Program recommended by the Deutsche Forschungsgemeinschaft (DFG). Samples are analyzed using GC-MS (selected ion monitoring) following acid hydrolysis, solid phase extraction, esterification with methanol/sulfuric acid, and liquid-liquid extraction. The metabolite, 3-phenoxybenzoic acid (3-PBA), can be derived from several pyrethroids and is, therefore, a useful diagnostic analyte; however, the presence of the over-the-counter drug, ibuprofen ((R,S)-2-(4-isobutylphenyl)propionic acid), interferes with this determination, even after the ingestion of only one 200-mg tablet. The interfering analyte is carboxy-ibuprofen which is not removed by the cleanup step. Experimental work shows that it takes two days for most of the ibuprofen to clear the body before 3-PBA can reliably be determined in urine.
Hardt
J
J
Institut für Laboratoriumsmedizin, Mikrobiologie und Umwelthygiene, Klinikum Augsburg, Germany. Ehret.ILMU@klinikum-augsburg.de
D000328:Adult; D001565:Benzoates / Q000652:urine*; D002849:Chromatography, Gas; D004781:Environmental Exposure / Q000032:analysis; D006801:Humans; D007052:Ibuprofen / Q000032:analysis*; D007306:Insecticides / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry; D011722:Pyrethrins / Q000652:urine*
D001565:Benzoates; D007306:Insecticides; D011722:Pyrethrins; C017618:3-phenoxybenzoic acid; D007052:Ibuprofen