Reporter-gene systems for the study of G-protein-coupled receptors.
Reporter-gene assays offer an alternative to biochemical assays for following signal transduction pathways from receptors at the cell surface to nuclear gene transcription in living cells. Specific reporter-gene systems are now available for the study of ligand activity at G alpha(i/o), G alpha(s) and G alpha(q) G-protein-coupled receptors. In recent years reporter genes have been applied in academia and industry to the study of ligand efficacy and affinity in recombinant and primary cell lines using a variety of colour, fluorescent or luminescent read-outs.
Hill
S J
SJ
Institute of Cell Signalling, Medical School, University of Nottingham, Queen's Medical Centre, UK. stephen.hill@nottingham.ac.uk
Baker
J G
JG
Rees
S
S
D000818:Animals; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D017930:Genes, Reporter; D005821:Genetic Techniques / Q000706:statistics & numerical data / Q000639:trends; D006801:Humans; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins