title: Growth differentiation factor-9 promotes the growth, development, and survival of human ovarian follicles in organ culture.
abstract: Growth differentiation factor-9 (GDF-9) is a growth factor and a member of the TGFbeta superfamily that is secreted by oocytes in growing ovarian follicles. In the current study we cultured human ovarian follicles within slices of ovarian cortical tissue in the presence and absence of recombinant rat GDF-9. Ovarian tissue was obtained by biopsy during cesarean section (31 women) or gynecological laparoscopy (3 women). The mean age (+/-SD) of the women who donated tissue was 32.8 +/- 5.0 yr (range, 19-41 yr). In these biopsies a total of 900 follicles were analyzed. A significantly higher proportion of cultured primordial follicles showed growth initiation and reached the secondary stage of development in the presence of GDF-9. In serum-free cultures after 7 d, 53% of the follicles had reached the secondary stage with GDF-9 vs. 31% in the control group (P < 0.01). Follicle viability was also improved in the presence of GDF-9 after 7 d in culture (74% GDF-9 vs. 48% control), which resulted in a smaller reduction in follicle numbers due to atresia. Treatment with GDF-9 in vitro promoted the survival and progression of human follicular development to the secondary stage. This oocyte-derived factor may be essential for the development of somatic cells in early human follicles and useful in designing culture conditions for maturation of follicles and oocytes in vitro.
authors.affiliation: Karolinska Institutet, Department of Obstetrics and Gynecology, Huddinge University Hospital, S 141 86 Huddinge, Sweden. julius.hreinsson@obgyn.hs.sll.se
authors.forename: Julius Gisli
authors.initials: JG
authors.lastname: Hreinsson
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Scott
authors.forename: Carsten
authors.initials: C
authors.lastname: Rasmussen
authors.forename: Marja Liisa
authors.initials: ML
authors.lastname: Swahn
authors.forename: Aaron J W
authors.initials: AJ
authors.lastname: Hsueh
authors.forename: Outi
authors.initials: O
authors.lastname: Hovatta
mesh_terms: D000328:Adult; D055430:Bone Morphogenetic Protein 15; D002470:Cell Survival; D005260:Female; D055429:Growth Differentiation Factor 9; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D009865:Oocytes / Q000166:cytology; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: C525901:BMP15 protein, human; C525904:Bmp15 protein, rat; D055430:Bone Morphogenetic Protein 15; C525897:GDF9 protein, human; C525899:Gdf9 protein, rat; D055429:Growth Differentiation Factor 9; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins