title: Generation of mesoscopic patterns of viable Escherichia coli by ambient laser transfer.
abstract: We have generated mesoscopic patterns of viable Escherichia coli on Si(1 1 1), glass, and nutrient agar plates by using a novel laser-based transfer process termed matrix assisted pulsed laser evaporation direct write (MAPLE DW). We observe no alterations to the E. coli induced by the laser-material interaction or the shear forces during the transfer. Transferred E. coli patterns were observed by optical and electron microscopes, and cell viability was shown through green fluorescent protein (GFP) expression and cell culturing experiments. The transfer mechanism for our approach appears remarkably gentle and suggests that active biomaterials such as proteins, DNA and antibodies could be serially deposited adjacent to viable cells. Furthermore, this technique is a direct write technology and therefore does not involve the use of masks, etching, or other lithographic tools.
authors.affiliation: Plasma Processing Section/Code 6372, Naval Research Laboratory, Washington, DC 20375, USA. ringeisn@ccs.nrl.navy.mil
authors.forename: B R
authors.initials: BR
authors.lastname: Ringeisen
authors.forename: D B
authors.initials: DB
authors.lastname: Chrisey
authors.forename: A
authors.initials: A
authors.lastname: Piqué
authors.forename: H D
authors.initials: HD
authors.lastname: Young
authors.forename: J
authors.initials: J
authors.lastname: Jones-Meehan
authors.forename: R
authors.initials: R
authors.lastname: Modi
authors.forename: M
authors.initials: M
authors.lastname: Bucaro
authors.forename: B J
authors.initials: BJ
authors.lastname: Spargo
mesh_terms: D004926:Escherichia coli / Q000166:cytology / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D007834:Lasers; D008164:Luminescent Proteins / Q000235:genetics; D008854:Microscopy, Electron
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins