[Down regulation of HER2/neu expression by adenovirus E1A and its anti-tumor activity].
OBJECTIVE
To study the growth inhibitory and chemo-sensitizing effects of adenovirus E1A gene on HER2/neu-overexpressing tumor cell lines.


METHODS
E1A was transfected in vitro and in vivo by adenovirus vector into HER2/neu overexpressing human mammary cancer cell lines MDA-MB-453 and SKBR3 and their growth was monitored. The chemo-sensitizing effect was examined by MTT assay.


RESULTS
E1A greatly inhibited growth of HER2/neu-overexpressing tumor and prolonged the survival time of tumor-bearing mice. Western blot and immunohistochemical analysis both showed suppression of p185 protein expression in E1A-transfected HER2/neu overexpressing cancer cell lines. E1A could sensitize HER2/neu-overexpressing human breast cancer cells to Taxotere by repressing HER2/neu expression.


CONCLUSIONS
Adenovirus E1A inhibits tumor growth and sensitizes tumor cells to chemotherapeutic agent via down regulation of HER2/neu expression.
Lu
H
H
Immunology Department, Shanghai Medical University, Shanghai 200032, China.
Qin
H
H
Zhang
Y
Y
D000256:Adenoviridae / Q000235:genetics; D017500:Adenovirus E1A Proteins / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000401:mortality; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
D017500:Adenovirus E1A Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases