title: [hCG-PLZF-RARA transgenic mice develop leukemia resembling human chronic myeloid leukemia].
abstract: OBJECTIVE
  In order to investigate the leukemogenic potential of PLZF-RARA fusion protein in vivo, hCG-PLZF-RARA transgenic mice were generated, in which PIZF-RARA fusion gene was driven by hCG promoter to express in myeloid cells of mice.
  
  
  METHODS
  Molecular cloning technology was used to construct hCG-PLZF-RARA gene. The genotype and phenotype of the hCG-PLZF-RARA transgenic mice were analyzed by PCR, RT-PCR, immunofluorescence, morphology of bone marrow (BM) cells, pathology and retinoic acid differentiation assays.
  
  
  RESULTS
  Six hCG-PLZF-RARA transgenic mice developed leukemia resembling human chronic myeloid leukemia. TF(tissue factor) was not expressed in BM cells of normal mice nor in mice without the expressed transgene, but it was expressed in mice expessing the transgene.
  
  
  CONCLUSIONS
  PLZF-RARA fusion protein plays a crucial role in leukemogenesis. TF is up-regulated by PLZF-RARA fusion gene.
authors.affiliation: Shanghai Hematology Research Institute, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: X
authors.initials: X
authors.lastname: Meng
authors.forename: G
authors.initials: G
authors.lastname: Cheng
authors.forename: L
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D006063:Chorionic Gonadotropin; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; C118318:PLZF-RARalpha fusion protein, human; C492209:PLZF-RARalpha fusion protein, mouse; D011993:Recombinant Fusion Proteins; D013925:Thromboplastin