title: [Detecting Epstein-Barr virus in nasopharyngeal carcinoma tissue using in situ polymerase chain reaction].
abstract: OBJECTIVE
  To develop a sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissue.
  
  
  METHODS
  The EBV BamHI-W fragment labeled with digoxigenin as probe was used to perform in situ hybridization and PCR in situ hybridization (in situ PCR).
  
  
  RESULTS
  The method of in situ PCR for the detection of EBV in NPC tissue was proved to be much more sensitive than in situ hybridization.
  
  
  CONCLUSIONS
  Hybridization in situ of PCR product is useful to detect EBV in NPC tissues.
authors.affiliation: Key Laboratory of Carcinogenesis of Chineses Ministry of Public Health, Hunan Medical University, Changsha, 410078, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Xie
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004279:DNA, Viral