title: Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater.
abstract: New cultured strains of the planctomycete division (order Planctomycetales) of the domain Bacteria related to species in the genera Gemmata and Isosphaera were isolated from soil, freshwater, and a laboratory ampicillin solution. Phylogenetic analysis of the 16S rRNA gene from eight representative isolates showed that all the isolates were members of the planctomycete division. Six isolates clustered with Gemmata obscuriglobus and related strains, while two isolates clustered with Isosphaera pallida. A double-membrane-bounded nucleoid was observed in Gemmata-related isolates but not in Isosphaera-related isolates, consistent with the ultrastructures of existing species of each genus. Two isolates from this study represent the first planctomycetes successfully cultivated from soil.
authors.affiliation: Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Jenny
authors.initials: J
authors.lastname: Wang
authors.forename: Cheryl
authors.initials: C
authors.lastname: Jenkins
authors.forename: Richard I
authors.initials: RI
authors.lastname: Webb
authors.forename: John A
authors.initials: JA
authors.lastname: Fuerst
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S