Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis.
OBJECTIVE
To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.


METHODS
An in vitro study.


METHODS
Department of Obstetrics and Gynecology, University of Aberdeen, UK.


METHODS
Women attending a fertility clinic.


METHODS
In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 microg/mL, 1 microg/mL, 10 microg/mL).


METHODS
In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.


RESULTS
There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis, IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 microg/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.


CONCLUSIONS
Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes.
Gazvani
Rafet
R
Department of Obstetrics and Gynaecology, University of Aberdeen, Scotland, Foresterhill, Aberdeen, UK. m.r.gazvani@abdn.ac.uk
Smith
Louise
L
Fowler
Paul A
PA
D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium / Q000166:cytology* / Q000187:drug effects / Q000473:pathology*; D005260:Female; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008183:Luteal Phase; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000473:pathology
D016209:Interleukin-8; D018664:Interleukin-12