title: Engineered collagen-PEO nanofibers and fabrics.
abstract: Type I collagen-PEO fibers and non-woven fiber networks were produced by the electrospinning of a weak acid solution of purified collagen at ambient temperature and pressure. As determined by high-resolution SEM and TEM. fiber morphology was influenced by solution viscosity, conductivity, and flow rate. Uniform fibers with a diameter range of 100-150 nm were produced from a 2-wt% solution of collagen-PEO at a flow rate of 100 microl min(-1). Ultimate tensile strength and elastic modulus of the resulting non-woven fabrics was dependent upon the chosen weight ratio of the collagen-PEO blend. 1H NMR dipolar magnetization transfer analysis suggested that the superior mechanical properties, observed for collagen-PEO blends of weight ratio 1:1, were due to the maximization of intermolecular interactions between the PEO and collagen components. The process outlined herein provides a convenient, non-toxic, non-denaturing approach for the generation collagen-containing nanofibers and non-woven fabrics that have potential application in wound healing, tissue engineering, and as hemostatic agents.
authors.affiliation: Department of Surgery, Emory University, Atlanta 30350, USA.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: K
authors.initials: K
authors.lastname: Nagapudi
authors.forename: R P
authors.initials: RP
authors.lastname: Apkarian
authors.forename: E L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D003094:Collagen / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D011092:Polyethylene Glycols; D003094:Collagen