title: Isolation and characterization of isolectins from Talisia esculenta seeds.
abstract: Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (Mr 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates.
authors.affiliation: Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (Unicamp), SP, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Freire
authors.forename: O L
authors.initials: OL
authors.lastname: Machado
authors.forename: M B
authors.initials: MB
authors.lastname: Smolka
authors.forename: S
authors.initials: S
authors.lastname: Marangoni
authors.forename: J C
authors.initials: JC
authors.lastname: Novello
authors.forename: M L
authors.initials: ML
authors.lastname: Macedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins; D051381:Rats; D029631:Sapindaceae / Q000196:embryology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D037102:Lectins; D037121:Plant Lectins