[RAB5A, a gene possibly related to metastasis of human carcinoma of the lung and stomach].
OBJECTIVE
To study and isolate tumor metastasis-related genes, and to explore the molecular basis of metastasis of lung and gastric carcinoma.


METHODS
Using mRNA differential display technique, cDNA cloning and sequencing, RT-PCR, Northern blot and immunocytochemical methods, genes differentially expressed between human lung adenocarcinoma cell lines AGZY83-a and Anip973 were detected. Anip973 was isolated from AGZY83-a with much higher metastatic potential than the parent line.


RESULTS
There were significant differences in gene expression between the two cell lines. RAB5A was one of the genes differentially expressed in Anip973 cells. Its gene product is a GTP-binding protein. Paraffin sections of 33 lung carcinoma and 47 gastric carcinoma specimens were examined immunohistochemically. Expression of RAB5A was positive in 87.9% of NSCLC and 91.5% of gastric cancer specimens examined (P < 0.05), and correlated with their metastatic potential and differentiation degree of gastric carcinoma.


CONCLUSIONS
RAB5A is possibly a metastasis-related gene.
Li
Y
Y
Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086.
Feng
H
H
Chen
Y
Y
D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology*; D009362:Neoplasm Metastasis / Q000235:genetics*; D013274:Stomach Neoplasms / Q000473:pathology*; D020696:rab5 GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*
D020696:rab5 GTP-Binding Proteins