title: [Application of sequential culture in assisted reproductive technique].
abstract: OBJECTIVE
  To investigate the effects of sequential culture technique on the outcomes of in vitro fertilization-embryo transfer(IVF-ET).
  
  
  METHODS
  Sequential culture media were used in 47 IVF-ET cycles, and conventional medium in 114 cycles. The fertilization rate, cleavage rate and speed, good embryo rate and pregnancy rate were compared between these 2 groups.
  
  
  RESULTS
  There were significantly higher embryo cleavage and quality score rate, pregnancy rate (97.9%, 64.4%, 46.7%) in sequential media group as compared those in conventional medium group (89.7%, 40.2%, 28.2%).
  
  
  CONCLUSIONS
  Sequential culture system is more suitable for the development of cultured human oocytes and embryos.
authors.affiliation: Center of Reproductive Medicine, Shandong Province Hospital, Jinan 250021, China.
authors.forename: M
authors.initials: M
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
mesh_terms: D001757:Blastomeres; D003470:Culture Media; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D006801:Humans; D027724:Reproductive Techniques, Assisted
chemical_list: D003470:Culture Media