OARE-1, a Ty1-copia retrotransposon in oat activated by abiotic and biotic stresses.
Transcriptionally active Ty1-copia LTR-retrotransposons were found in oat using RT-PCR for amplifying the reverse transcriptase domain. Sequence analysis of the RT-PCR clones suggested that oat LTR-retrotransposons consist of at least seven groups, which were tentatively designated as Oatrt1 to Oatrt7. A full length copy of Oatrt1 was isolated from an oat genomic library, and was designated OARE-1. OARE-1 was 8,665 bp long and a member of the BARE-1 subgroup. The oat genome carried it in multiple copies (at least 10,000 copies / a hexaploid genome). The expression of OARE-1 was intensively induced by wounding, UV light, jasmonic acid and salicylic acid, and its pattern was very similar to that of the PAL (phenylalanin ammonia lyase) gene. Furthermore, OARE-1 was highly activated by infection with an incompatible race of the crown rust fungus, Puccinia coronata. These results suggest that OARE-1 is highly sensitive to various abiotic and biotic stimuli leading to plant defense responses.
Kimura
Y
Y
Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe, 657-8501 Japan.
Tosa
Y
Y
Shimada
S
S
Sogo
R
R
Kusaba
M
M
Sunaga
T
T
Betsuyaku
S
S
Eto
Y
Y
Nakayashiki
H
H
Mayama
S
S
D000595:Amino Acid Sequence; D018554:Avena / Q000235:genetics* / Q000382:microbiology / Q000502:physiology; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D005658:Fungi / Q000254:growth & development; D015870:Gene Expression; D008969:Molecular Sequence Data; D054883:Oxylipins; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018626:Retroelements / Q000235:genetics* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015533:Transcriptional Activation; D014466:Ultraviolet Rays
C082800:BARE-1 protein, Hordeum vulgare; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D018626:Retroelements; C011006:jasmonic acid; D010650:Phenylalanine Ammonia-Lyase; D020156:Salicylic Acid