title: Automated quantitation of hemoglobin-based blood substitutes in whole blood samples.
abstract: It is necessary to develop methods for accurate monitoring of cell-free hemoglobin in circulation. Routine monitoring of circulating cell-free hemoglobin will be useful for evaluating the efficacy of blood substitute administration andfor determining the clearance rates of the blood substitute from circulation. In addition, discriminating between cell-free hemoglobin and cell-associated hemoglobin will enable accurate determination of RBC indices, mean cell hemoglobin and mean corpuscular hemoglobin concentration, in individuals receiving hemoglobin-based blood substitutes. As colorimetric methods used by hematology analyzers to quantitate the hemoglobin value of a blood sample cannot distinguish between cell-associated and cell-free hemoglobin, it is currently not feasible to quantitate the levels of hemoglobin substitutes in circulation. The advent of a technology that measures volume and hemoglobin concentration of individual RBCs provides an alternative strategy for quantitating the cell-associated hemoglobin in a blood sample. We document that the combined use of cell-based and colorimetric hemoglobin measurements provides accurate discrimination between cell-associated and cell-free hemoglobin over a wide range of hemoglobin levels. This strategy should enable rapid and accurate monitoring of the levels of cell-free hemoglobin substitutes in the circulation of recipients of these blood substitutes.
authors.affiliation: Laboratory Testing Segment, Bayer, Tarrytown, NY, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kunicka
authors.forename: M
authors.initials: M
authors.lastname: Malin
authors.forename: D
authors.initials: D
authors.lastname: Zelmanovic
authors.forename: M
authors.initials: M
authors.lastname: Katzenberg
authors.forename: W
authors.initials: W
authors.lastname: Canfield
authors.forename: P
authors.initials: P
authors.lastname: Shapiro
authors.forename: N
authors.initials: N
authors.lastname: Mohandas
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000097:blood; D000755:Anemia, Sickle Cell / Q000097:blood; D001802:Blood Substitutes / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry*; D006452:Hemoglobinometry / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008027:Light; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013789:Thalassemia / Q000097:blood
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins