[Chronically HIV-1 infected human monocyte culture U937 as a model for assessing the efficacy of anti-HIV agents].
Cell culture U937 chronically infected with HIV-1 is suggested as a model for adequate evaluation of antiviral activity of HIV inhibitors. Azidothimidine (AZT) notable decreased HIV-1 reproduction in chronically infected U937 cells to passages 15-18. Glycirrhizic acid (GA) effectively inhibited the virus production during the first four passages, while in subsequent passages (up to passage 20) decreased the virus production by only 60% in comparison with the control. If a combination of AZT and GA (1:1000) was used, p24 was not detected in the culture fluid by passage 20. Culturing of U937 cells with AZT led to a 10-fold decrease in the amount of DNA 2-LTR in comparison with the total content of proviral DNA, the content of HIV-1 DNA 1-LTR remaining virtually unchanged. Culturing of U937 with a combination of AZT and GA resulted in a notable decrease in the content of proviral DNA 2-LTR after passage 3, while after passage 9 this form of HIV-1 DNA was not detected at all.
Pokrovskiĭ
A G
AG
Pliasunova
O A
OA
Gashnikova
N M
NM
Mamaeva
O A
OA
Fediuk
N V
NV
D019380:Anti-HIV Agents / Q000494:pharmacology*; D019695:Glycyrrhizic Acid / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology; D012692:Serial Passage; D020298:U937 Cells; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D019695:Glycyrrhizic Acid
Khronicheski infitsirovannaia VICH-1 kul'tura monotsitov cheloveka U937 kak model' dlia otsenki éffektivnosti anti-VICh-preparatov.