title: An erythroid-specific chromatin opening element reorganizes beta-globin promoter chromatin structure and augments gene expression.
abstract: In erythroid tissues the chromatin structure of the beta-globin gene locus is extensively remodeled. Changes include the formation of DNase I hypersensitive sites (HSs) over the promoters of actively expressed genes. To test the hypothesis that such "opening" of promoter chromatin structure is important for beta-globin gene expression, we placed a 101-bp erythroid-specific hypersensitive-site forming element (HSFE) from the core of LCR HS4 immediately upstream of a minimal beta-globin gene promoter. We then studied the effects of this element alone and in combination with other cis-acting elements on globin gene chromatin structure and gene expression in MEL cells and transgenic mice. Single or tandem HSFEs increased the size of the portion of the promoter accessible to DNase digestion, increased the proportion of promoters in an accessible conformation, and increased gene expression approximately 5-fold. These were equivalent to expression levels attained using a 2.8-kb microLCR construct. Inclusion of the LCR HS2 enhancer did not increase expression further. In transgenic mouse fetal liver cells the HSFE increased average expression 2.5-fold compared to the minimal promoter alone. These results indicate that a small cis-acting element is capable of remodeling local beta-globin promoter chromatin structure and producing expression similar to that seen with a microLCR construct.
authors.affiliation: Department of Pharmacology & Toxicology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Nemeth
authors.forename: D M
authors.initials: DM
authors.lastname: Bodine
authors.forename: L J
authors.initials: LJ
authors.lastname: Garrett
authors.forename: C H
authors.initials: CH
authors.lastname: Lowrey
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics* / Q000648:ultrastructure; D004274:DNA, Recombinant / Q000187:drug effects / Q000235:genetics; D003850:Deoxyribonuclease I / Q000494:pharmacology; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D019901:Locus Control Region / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D002843:Chromatin; D004274:DNA, Recombinant; D012333:RNA, Messenger; D005914:Globins; D003850:Deoxyribonuclease I