[Identification and molecular characterization of a Toxoplasma gondii microneme].
Protozoan of the phylum Apicomplexa are of high medical and veterinary importance, causing diseases such as malaria, toxoplasmosis and cryptosporidiosis. Invasive stages of apicomplexans possess organelles named micronemes, which are involved in the invasion process. We have recently characterized a protein in micronemes of Toxoplasma gondii, TgMIC3, which possess adhesive properties to host cell surface. Immunofluorescence analysis of T. gondii tachyzoite invasion showed that TgMIC3 is exocytosed and re-localised on the surface of the parasite during invasion. By being able to bind both the putative host cells and the parasites, TgMIC3 could be involved in invasion by acting as a bridge between the parasite and the host cell. Gene sequence analysis of TgMIC3 has revealed 5 partially overlapping EGF-like domains and a lectin binding-like domain, which can be involved in protein-protein or protein-carbohydrate interactions respectively. TgMIC3 is a homodimer synthetized with a N-terminal propeptide that is cleaved during trafficking to the organelle, presumably in the trans-Golgi network. The processing involves a serine protease and is required for correct binding function of TgMIC3. The exact role of this propeptide remains unexplained. It may be involved in the targetting of the protein to the micronemes by masking the region involved in interaction with membranes to avoid binding of the protein in the trafficking pathway.
Cérède
O
O
UMR Université INRA d'Immunologie Parasitaire, Faculté de Pharmacie de Tours, 31, avenue Monge, F37200 Tours.
Garcia-Réguet
N
N
Conseil
V
V
Bout
D
D
Dubremetz
J F
JF
Lebrun
M
M
D018829:Adhesins, Bacterial; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D002448:Cell Adhesion; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics; D014122:Toxoplasma / Q000737:chemistry* / Q000235:genetics
D018829:Adhesins, Bacterial; D002352:Carrier Proteins; C421382:MIC3 protein, Toxoplasma gondii; D015800:Protozoan Proteins
Mise en évidence et caractérisation moléculaire d'une adhésine (TgMIC3) des micronèmes de Toxoplasma gondii.