Flash detection/identification of pathogens, bacterial spores and bioterrorism agent biomarkers from clinical and environmental matrices.
We propose to develop an integrated rapid, semiportable, prototype point microbial detection/identification system for clinical specimens that is also capable of differentiating microbial bioterrorism attacks from threats or hoaxes by defining the pathogen. The system utilizes "flash" extraction/analytical system capable of detection/identification of microbes from environmental and clinical matrices. The system couples demonstrated technologies to provide quantitative analysis of lipid biomarkers of microbes including spores in a system with near-single cell (amol/microl) sensitivity. Tandem mass spectrometry increases specificity by providing the molecular structure of neutral lipids, phospholipids, and derivatized spore-specific bacterial biomarker, 2,6-dipicolinic acid (DPA) as well as the lipopolysaccharide-amide-linked hydroxy-fatty acids (LPS-ALHFA) of Gram-negative bacteria. The extraction should take about an hour for each sample but multiple samples can be processed simultaneously.
White
David C
DC
Center for Environmental Biotechnology, University of Tennessee, 10515 Research Drive, Suite 300, Knoxville, TN 37932-2575, USA. MILIPIDS@AOL.COM
Lytle
Cory A
CA
Gan
Ying-Dong M
YD
Piceno
Yvette M
YM
Wimpee
Michael H
MH
Peacock
Aaron D
AD
Smith
Carol A
CA
D001419:Bacteria / Q000737:chemistry* / Q000302:isolation & purification*; D015415:Biomarkers; D023482:Bioterrorism; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000032:analysis*; D013171:Spores, Bacterial; D013261:Sterols / Q000032:analysis; D014451:Ubiquinone / Q000032:analysis
D015415:Biomarkers; D004075:Diglycerides; D008055:Lipids; D013261:Sterols; D014451:Ubiquinone