title: Enzyme activity and protein content of superoxide dismutase isozymes in human renal cell carcinoma.
abstract: Alterations in renal superoxide dismutase (SOD) isozymes were examined in cancerous tissues of human renal cell carcinoma and the corresponding non-cancerous renal tissues. Cu,Zn-SOD activities in cancerous tissues were lower than those in normal tissues. Mn-SOD activities were varied in the cases examined, whereas no significant difference between cancerous and normal tissues was observed for Mn- or total-SOD activities. Immunoblot analysis showed that the loss in enzyme activity in cancerous tissue was greater than the decrease in protein content for either isozyme. The selective decrease in Cu,Zn-SOD activities in cancerous tissue observed in this study suggests that the cytoplasmic defense against free radical damage appears to be reduced in renal cell carcinoma.
authors.affiliation: Graduate School Doctoral Program in Medical Sciences, Institute of Community Medicine, University of Tsukuba, Ibaraki , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Homma-Takeda
authors.forename: A
authors.initials: A
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Kikushima
authors.forename: Y
authors.initials: Y
authors.lastname: Kumagai
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.forename: H
authors.initials: H
authors.lastname: Akaza
authors.forename: N
authors.initials: N
authors.lastname: Shimojo
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D007680:Kidney Neoplasms / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D009363:Neoplasm Proteins; D013482:Superoxide Dismutase