title: Characterization of DNA immobilized on electrochemically prepared conducting polypyrrole-polyvinyl sulfonate films.
abstract: The article describes the adsorption characteristics of DNA onto electrochemically generated polypyrrole-polyvinyl sulfonate (PPY-PVS) films obtained as a function of pH. Adsorption on PPY doped with an anion proceeds by anion exchange, and since DNA possesses a fixed negative charge owing to PO4-, it favors a very strong binding displacing PVS with favorable energetic interactions. Characterization of adsorbed DNA onto the PPY-PVS films was carried out by ultraviolet-visible, Fourier transform infrared spectroscopy, and cyclic voltammetric studies.
authors.affiliation: Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India. anamikagulati@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Gambhir
authors.forename: M
authors.initials: M
authors.lastname: Gerard
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
authors.forename: B D
authors.initials: BD
authors.lastname: Malhotra
mesh_terms: D000327:Adsorption; D000818:Animals; D000838:Anions; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011485:Protein Binding; D011758:Pyrroles / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry*; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D000838:Anions; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D013451:Sulfonic Acids; C008959:lyapolate; C067635:polypyrrole; D004247:DNA