Fluorescence studies on native and bound to trifluraline soy bean Lb"a" in the enhanced N2 fixation.
The differences in the tryptophan (Trp) fluorescence of native (control) Lb"a" and experimental substance isolated from nodules of the Williams' soy beans variety treated with trifluraline at a concentration of 2.1 x 10(-10) M have been studied. A positively charged environment has been proved for the tryptophans of the native Lb"a" and a negative one for the tryptophans of the experimental Lb"a". The difference in the tryptophan emission spectra at lambdaex = 280 and 300 nm may be assigned to conformational alterations occurring in the experimental Lb"a". This is also confirmed by the greater energy transfer from tyrosine to tryptophan in the experimental Lb"a"--30% compared to the 10% in the native Lb"a". The value of the constant of acrylamide quenching (Ksv = 2.77 M(-1)) shows that the tryptophans are buried more deeply in the experimental Lb"a" than in the native Lb"a" (Ksv = 4 M(-1)). They are substantially lower than Ksv of the standard compound N-Ac-Trp-NH2 (16.30 M(-1)). The activation energy (Ea) of the thermal quenching of tryptophan fluorescence is higher for the experimental Lb"a" (37 kJ mol(-1)) as compared to the standard compound N-Ac-Trp-NH2 (24 kJ mol(-1)) and the native Lb "a" (32 kJ mol(-1)). The dissociation constant of the complex of trifluraline with Lb "a" (6.32 x 10(-11) M) has been determined as well as the stoichiometric ratio trifluraline/Lb"a" (1:1). The estimated nitrogenase activity (microM/gfrw h) and the total Lb (mg/gfrw) for trifluraline are higher as compared to those for the control.
Kolev
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K
Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia. ifrlib@obzor.bio21.bas.bg
Dolashka-Angelova
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D000178:Acrylamides / Q000737:chemistry; D004735:Energy Transfer; D006540:Herbicides / Q000378:metabolism* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007874:Leghemoglobin / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008968:Molecular Conformation; D009586:Nitrogen Fixation; D013025:Glycine max / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014274:Trifluralin / Q000378:metabolism* / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry; D014443:Tyrosine / Q000737:chemistry
D000178:Acrylamides; D006540:Herbicides; D007874:Leghemoglobin; D008024:Ligands; D014443:Tyrosine; D014364:Tryptophan; D014274:Trifluralin