title: PCR ribotyping of clinically important Clostridium difficile strains from Hungary.
abstract: Isolates of Clostridium difficile from different hospital wards at the University Hospital of Szeged in Hungary were typed by PCR amplification of rRNA intergenic spacer regions (PCR ribotyping). A total of 15 different ribotypes was detected among the 65 isolates tested. The predominant type, PCR ribotype 087, accounted for 39% of all isolates, in contrast with an international typing study where ribotype 001 was the most common. Two non-toxigenic C. difficile strains were found to exhibit the same pattern, which was distinct from those of all the ribotypes described previously, suggesting that this is a new type.
authors.forename: Edit
authors.initials: E
authors.lastname: Urbán
authors.affiliation: Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725 Szeged, Hungary and *PHLS Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University of Wales College of Medicine, Cardiff CF14 4XN.
authors.forename: Jon S
authors.initials: JS
authors.lastname: Brazier
authors.forename: József
authors.initials: J
authors.lastname: Sóki
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Nagy
authors.affiliation: Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725 Szeged, Hungary and *PHLS Anaerobe Reference Unit, Department of Medical Microbiology and Public Health Laboratory, University of Wales College of Medicine, Cardiff CF14 4XN.
authors.forename: Brian I
authors.initials: BI
authors.lastname: Duerden
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D002522:Chlorocebus aethiops; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003428:Cross Infection; D004761:Enterocolitis, Pseudomembranous / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005784:Gene Amplification; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction / Q000379:methods; D012335:RNA, Ribosomal / Q000235:genetics*; D021521:Ribotyping; D014709:Vero Cells
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D012335:RNA, Ribosomal; C031815:toxB protein, Clostridium difficile