[Experimental study of Bcl-2 and Fas gene expression in fibroblast of scar].
OBJECTIVE
To explore the effect of Fas/Apo-1 and Bcl-2 gene expression on mechanism of scar formation.


METHODS
Immunohistochemical method was applied to defect the expression of Fas and Bcl-2 protein in fibroblasts from 10 cases with normal skin, 10 cases with hypertrophic scar and 10 cases with keloid.


RESULTS
The positive expression rate of Bcl-2 protein in keloid was 83.2%, significantly higher than that in hypertrophic scar (38.6%), (P < 0.01), and the positive expression rate in hypertrophic scar and keloid was higher than that in normal skin (6.78%), (P < 0.01). But the positive expression rate of Fas/Apo-1 protein was 78.4% in normal skin 80.4% in hypertrophic scar, 84.4% in keloid respectively, which showed no significant difference among them (P > 0.05).


CONCLUSIONS
Bcl-2 gene but Fas gene may take part in the formation of pathologic scar.
Liu
Y
Y
Department of Burn and Plastic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu Sichuan, P. R. China 610041.
Ren
L S
LS
Cen
Y
Y
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007627:Keloid / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis* / Q000235:genetics; D012867:Skin / Q000378:metabolism / Q000473:pathology; D019014:fas Receptor / Q000096:biosynthesis* / Q000235:genetics
D019253:Proto-Oncogene Proteins c-bcl-2; D019014:fas Receptor