[Expression of heat shock protein 90 alpha and its effect on the growth of tumor in mice].
OBJECTIVE
To study the effect of hsp90 alpha on the growth of tumor in mice and its mechanism.


METHODS
Hsp90 alpha fusion protein was expressed in E. coli. DBA/2 mice were injected with purified fusion protein, then challenged with L1210 cells. The size of tumor was measured every other day. NK cell activity was detected by 51Cr release assay.


RESULTS
The tumors grew faster while activity NK cell activity of mice was lower in the test mice than that in the control mice.


CONCLUSIONS
The results suggest that the accelerated tumor growth is probably due to the formation of anti-hsp90 alpha antibody and decreased NK cell activity of mice treated with hsp90 alpha.
Qiao
H
H
Cancer Institute, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Zhao
M
M
Yuan
X
X
D000818:Animals; D018841:HSP90 Heat-Shock Proteins / Q000276:immunology / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*
D018841:HSP90 Heat-Shock Proteins