Detection of four human milk groups with respect to Lewis-blood-group-dependent oligosaccharides by serologic and chromatographic analysis.
Oligosaccharides from human milk samples obtained from individual donors were analyzed using high-pH anion-exchange chromatography. Three patterns of neutral oligosaccharides were detected corresponding to milk groups already described. These oligosaccharide groups correspond to the Lewis blood types Le(a-b+), Le(a+b-), and Le(a-b-). A new carbohydrate pattern was detected in a milk sample from a Le(a-b-) person in which only nonfucosylated oligosaccharides and compounds bearing alpha1,3-linked fucosyl residues were found. This finding led to the hypothesis that there exist 4 different oligosaccharide milk groups that fit well to the genetic basis of the Lewis blood group system.
Stahl
B
B
Numico Research Germany, Friedrichsdorf.
Thurl
S
S
Henker
J
J
Siegel
M
M
Finke
B
B
Sawatzki
G
G
D000838:Anions; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D005260:Female; D005643:Fucose / Q000032:analysis / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007785:Lactose / Q000032:analysis; D007983:Lewis Blood Group Antigens / Q000235:genetics; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D000838:Anions; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D005643:Fucose; D007785:Lactose