title: Agreia bicolorata gen. nov., sp. nov., to accommodate actinobacteria isolated from narrow reed grass infected by the nematode Heteroanguina graminophila.
abstract: Agreia bicolorata gen. nov., sp. nov. (type strain VKM Ac-1804T=UCM Ac-620T) is proposed to accommodate aerobic, oxidase- and catalase-positive, weakly motile, coryneform actinobacteria isolated from leaf galls induced by the plant-parasitic nematode Heteroanguina graminophila in narrow reed grass, Calamagrostis neglecta. Bacteria assigned to Agreia bicolorata gen. nov., sp. nov. form a distinct lineage within the phylogenetic branch of the family Microbacteriaceae and possess the following chemotaxonomic characteristics: B-type peptidoglycan containing 2,4-diaminobutyric acid, ornithine, alanine, glycine, glutamate and hydroxyglutamate; cell wall sugars rhamnose, fucose and mannose; MK-10 as major menaquinone; phosphatidylglycerol and diphosphatidylglycerol as principal phospholipids; and 12-methyltetradecanoic acid (anteiso-15:0), 14-methyl-pentadecanoic acid (iso-16:0) and 14-methyl-hexadecanoic acid (anteiso-17:0) as predominant fatty acids. The DNA G+C content of Agreia bicolorata is about 67.0 mol %.
authors.forename: L I
authors.initials: LI
authors.lastname: Evtushenko
authors.forename: L V
authors.initials: LV
authors.lastname: Dorofeeva
authors.forename: T G
authors.initials: TG
authors.lastname: Dobrovolskaya
authors.forename: G M
authors.initials: GM
authors.lastname: Streshinskaya
authors.forename: S A
authors.initials: SA
authors.lastname: Subbotin
authors.forename: J M
authors.initials: JM
authors.lastname: Tiedje
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D009348:Nematoda / Q000502:physiology; D010802:Phylogeny; D010941:Plant Tumors / Q000382:microbiology* / Q000469:parasitology; D006109:Poaceae / Q000382:microbiology* / Q000469:parasitology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S