[The transfer and expression of human clotting factor IX in muscle mediated by electroporation].
OBJECTIVE
To probe into the feasibility of increasing hFIX cDNA transfer and expression in muscle.


METHODS
The high-frequency electric field was used to promote both Lac-Z-encoding plasmid pCMV beta and hFIX-expressing plasmid G1NaMCIX to transfer and express in muscle. The effects of frequency and length of square pulse, as well as eletroporation time on hFIX expression were investigated.


RESULTS
Electric stimulation could increase the transfer and expression of pCMV beta in muscle, the number of X-gal positive myofiber cells in electroporation-treated mice is 2.1 times larger than that of mice not treated by electroporation (P<0.01). The most optimal electric simulation condition for hFIX cDNA transfer and expression was obtained, under this condition, the highest level of hFIX antigen in plasma is (40+/- 5.4) ng/ml and 7 times higher than that of mice without electroporation P < 0.001).


CONCLUSIONS
Electroporation is able to enhance hFIX cDNA transfer and expression in muscle efficiently.
Wang
H
H
School of Sciences, Institute of Genetics, Fudan University, Shanghai, 200433 P.R.China. darlu@fudan.edu.cn
Wang
F
F
Chen
L
L
Xue
J
J
Lu
D
D
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D005164:Factor IX / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006801:Humans; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics
D018076:DNA, Complementary; D005164:Factor IX