Isolation of a novel agglutinin with complex carbohydrate binding specificity from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji.
A hemagglutinin, with a molecular weight of 30,000 and expressing hemagglutinating activity which could not be inhibited by simple sugars and glycoproteins, was isolated from fresh fruiting bodies of the edible mushroom Lyophyllum shimeiji. The protein was adsorbed on CM-Sepharose even in 20 mM ammonium acetate (pH 5.5) containing 1 M NaCl and was desorbed by 20 mM ammonium bicarbonate (pH 9). The hemagglutinating activity was subsequently adsorbed on Mono S in 20 mM ammonium acetate (pH 5.5) and was desorbed by a linear gradient of 0.2-0.5 M NaCl in ammonium acetate buffer. The hemagglutinin exhibited a novel N-terminal sequence not found in any lectin and hemagglutinin reported so far. It was devoid of antifungal activity.
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T B
TB
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, People's Republic of China.
Lam
Y W
YW
D000085:Acetates / Q000494:pharmacology; D000373:Agglutinins / Q000302:isolation & purification* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012685:Sepharose / Q000737:chemistry; D012965:Sodium Chloride / Q000494:pharmacology
D000085:Acetates; D000373:Agglutinins; D037102:Lectins; D012965:Sodium Chloride; D012685:Sepharose; C018824:ammonium acetate