title: [Formation of artificial nitrogen-fixing symbiosis with rape (Brassica napus var. napus) plants in nonsterile soil].
abstract: The treatment of rape plants grown in nonsterile soil with 2,4-dichlorophenoxyacetic acid (auxin-like growth-promoting substance) or their inoculation with the bacterial association Micrococcus sp. + Rhodococcus sp. and/or with the mixed nitrogen-fixing culture Azotobacter nigricans + Bacillus sp. led to the formation of paranodules on the rape roots. The introduced bacteria were detected both in the intercellular space and inside the cells of the paranodules and the rape roots. The nitrogen-fixing activity of the paranodulated plants was two times higher than that of the inoculated plants lacking paranodules and five times higher than that of the control (i.e., not inoculated) plants. The paranodulation led to a 40% increase in the crop yield of rape plants and provided for a statistically significant increase in the total nitrogen as well as protein nitrogen contents of the plants.
authors.affiliation: Department of Soil Biology, Faculty of Soil Science, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Koval'skaia
authors.forename: E S
authors.initials: ES
authors.lastname: Lobakova
authors.forename: M M
authors.initials: MM
authors.lastname: Umarov
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001395:Azotobacter / Q000502:physiology; D001407:Bacillus / Q000502:physiology; D029688:Brassica napus / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology*; D008837:Micrococcus / Q000502:physiology; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology*; D012240:Rhodococcus / Q000502:physiology; D013559:Symbiosis
chemical_list: D015084:2,4-Dichlorophenoxyacetic Acid
vernacular_title: Formirovanie iskusstvennogo azotfiksiruiushchego simbioza u rasteniĭ rapsa (Brassica napus var. napus) v nesteril'noĭ pochve.