title: [Relationship between lectin, alpha-, beta-glucosidase, and beta-galactosidase activities of Azospirillum].
abstract: The activities of alpha-glucosidase, beta-glucosidase, and beta-galactosidase were studied during the isolation and purification of lectins from Azospirillum brasilense Sp7 and Azospirillum lipoferum 59b cells. These enzymatic activities were revealed in crude extracts of surface proteins, protein fraction precipitated with ammonium sulfate or ethanol-acetone mixture, and protein fraction obtained by gel filtration on Sephadex G-75. The distribution of the enzymes between different protein fractions varied among the azospirilla studied. The cofunction of the A. brasilense Sp7 lectin and beta-galactosidase on the cell surface is assumed. A strong interaction between the A. lipoferum 59b lectin and glucosidases was revealed. The lectin from A. lipoferum 59b may possess saccharolytic activity.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
authors.forename: S A
authors.initials: SA
authors.lastname: Alen'kina
authors.forename: V E
authors.initials: VE
authors.lastname: Nikitina
authors.forename: M V
authors.initials: MV
authors.lastname: Borisova-Golovko
mesh_terms: D016949:Azospirillum / Q000737:chemistry / Q000201:enzymology*; D015806:Azospirillum brasilense / Q000737:chemistry / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D002850:Chromatography, Gel; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D001616:beta-Galactosidase / Q000302:isolation & purification / Q000378:metabolism*; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D037102:Lectins; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase
vernacular_title: Izuchenie vzaimosviazi lektinovoĭ, al'fa-, beta-gliukozidaznykh i beta-galaktozidaznoĭ aktivnosteĭ azospirill.