Correlation between decreased expression of mitochondrial F0F1-ATP synthase and low regenerating capability of the liver after partial hepatectomy in hypothyroid rats.
In hypothyroid rats, partial hepatectomy does not induce liver regeneration until 120 h after surgical operation. when, instead, in normal rats a complete recovery of the liver mass, in this interval, is observed. In normal rats, a good efficiency of mitochondrial oxidative phosphorylation is needed as an energy source for liver regeneration (Guerrieri, F. et al., 1995); in hypothyroid rats the efficiency of mitochondrial oxidative phosphorylation is low in the 0-120 h interval after partial hepatectomy. This low efficiency of oxidative phosphorylation appears to be related to a low mitochondrial content of F0F1-ATP synthase, in liver of hypothyroid rats, which does not recover after partial hepatectomy. In the liver of hypothyroid rats, low levels of the nuclear-encoded mitochondrial catalytic betaF1 subunit and of its transcript are observed and they do not increase, as occurs in normal rats, after partial hepatectomy.
Guerrieri
F
F
Department of Medical Biochemistry and Biology and Centre for the Study of Mitochondria and Energy Metabolism (CNR), University of Bari, Italy. fer.guer@biochem.uniba.it
Nicoletti
C
C
Adorisio
E
E
Caraccio
G
G
Leonetti
P
P
Zanotti
F
F
Cantatore
P
P
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D013956:Antithyroid Agents / Q000009:adverse effects; D004195:Disease Models, Animal; D015870:Gene Expression; D006498:Hepatectomy; D007037:Hypothyroidism / Q000139:chemically induced / Q000201:enzymology*; D008099:Liver / Q000201:enzymology / Q000502:physiology; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D011441:Propylthiouracil / Q000009:adverse effects; D006180:Proton-Translocating ATPases / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D013956:Antithyroid Agents; D011441:Propylthiouracil; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases