Isolation and characterization of isolectins from Talisia esculenta seeds.
Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (Mr 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates.
Freire
M G
MG
Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (Unicamp), SP, Brazil.
Machado
O L
OL
Smolka
M B
MB
Marangoni
S
S
Novello
J C
JC
Macedo
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006801:Humans; D007525:Isoelectric Focusing; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D037121:Plant Lectins; D051381:Rats; D029631:Sapindaceae / Q000196:embryology*; D012639:Seeds / Q000737:chemistry*
D037102:Lectins; D037121:Plant Lectins