Isolation and evaluation of Coxiella burnetii O-polysaccharide antigen as an immunodiagnostic reagent.
A lipid A - deprived lipopolysaccharide (LPS) from Coxiella burnetii (C.b.) Priscilla strain in virulent phase I was separated by steric-exclusion chromatography and high performance liquid chromatography (HPLC). The isolated O-specific polysaccharide (PS) fractions were analyzed by different physico-chemical methods and showed noticeable differences in their overall composition. The antigenic potential of the PS fractions was evaluated by ELISA with animal and human sera and in comparison with those of the native C.b. LPSs and phase I and II C.b. Nine Mile stain cells of which the latter are routinely used in diagnosis of Q fever. The results indicate that the high molecular mass PS antigen SG501 could be used in ELISA for a sensitive and specific detection of anti-C.b. antibodies in the examined sera.
Hussein
A
A
Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Kovácová
E
E
Toman
R
R
D000907:Antibodies, Bacterial / Q000097:blood; D002690:Chlamydia Infections / Q000276:immunology; D016997:Coxiella burnetii / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D019081:O Antigens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D011778:Q Fever / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D019081:O Antigens