title: Platelet adhesion and activation on a shielded plasma gradient prepared on polyethylene.
abstract: Contact of blood with foreign materials evokes thrombogenic effects to an extent determined partly by the wettability of the biomaterials surface. Tools to study blood response towards a variation in materials wettability with minimal variation in chemistry are "gradient surfaces". However, most gradients have been prepared by diffusion or density immersion techniques, which results in a limited gradient range. Through glow discharge with partial shielding, gradients on polymers were prepared over a length of 5 cm, which facilitated studies to platelet adhesion on separate gradient sections. On polyethylene, advancing water contact angles varied from 90 degrees to 40 degrees, with a hysteresis of 30 degrees. ESCA indicated an increasing incorporation of oxygen towards the hydrophilic end. To examine the role of materials wettability on the activation of adhering platelets, sections of shielded plasma gradients were incubated in anticoagulated whole human blood. Fewer platelets adhered to the hydrophobic end, but those platelets were more activated than those on the hydrophilic end, as judged from their morphology and exposure of GpIIb-IIIa complex. However, partly related to the increased binding of platelets, the clotting activation after platelet deposition was highest on the hydrophilic end. Concluding, this new technique results in a large gradient range, which facilitates studies of formed blood elements in relation to the wettability. Platelets are more activated on hydrophobic polyethylene, while on moderate hydrophilic polyethylene more platelet adhesion and activation of the clotting system occurs.
authors.affiliation: Department of Biomedical Engineering, University of Groningen, The Netherlands. h.t.spijker@med.rug.nl
authors.forename: Hetty T
authors.initials: HT
authors.lastname: Spijker
authors.forename: Rolf
authors.initials: R
authors.lastname: Bos
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
authors.forename: TheoG
authors.initials: T
authors.lastname: van Kooten
authors.forename: Wim
authors.initials: W
authors.lastname: van Oeveren
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D001790:Blood Physiological Phenomena; D001792:Blood Platelets / Q000502:physiology / Q000648:ultrastructure; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D005170:Factor X / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology*; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology*; D020959:Polyethylene / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005170:Factor X; D020959:Polyethylene; D015951:Factor Xa