Direct electrochemistry of catalase on glassy carbon electrodes.
Catalase was investigated as a possible catalyst of the electrochemical reduction of oxygen on glassy carbon electrodes. The presence of catalase dissolved in solution only provoked a moderate current increase, which was fully explained by the catalase-catalysed disproportionation of hydrogen peroxide (Scheme I). When catalase was adsorbed from dimethylsulfoxide on the surface of electrodes that did not undergo any electrochemical pre-treatment (EP), catalase efficiently catalysed oxygen reduction via direct electron transfer from the electrode (Scheme II). The results are discussed with respect to the electrode surface properties and the enzyme structure.
Lai
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ME
Laboratoire de Génie Chimique, CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France.
Bergel
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D000327:Adsorption; D002244:Carbon; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D013499:Surface Properties
D004800:Enzymes, Immobilized; D002244:Carbon; D002374:Catalase; D004121:Dimethyl Sulfoxide