title: [ATPase activity of Neisseria gonorrhoeae and Staphylococcus aureus strains].
abstract: It has been established that membrane vesicles of gonococci possess ATPase activity within 0.05-0.14 mmol of ATP during 60 min per 1 mg of protein and for staphylococcus--within 0.07-0.19 mmol of ATP for 60 min per 1 mg of protein. The identity in all the kinetic parameters is observed for all the studied gonococcus strains: Na+ and Ca2+ ions inhibit ATPase activity within 12-19%; Mg2+ ions increase the ATPase activity 2-2.5 times. Dicyclohexylcarboimide, a specific ATPase inhibitor, suppresses ATPase activity by 50-70%. ATPase activity in the strains of bacteria containing antibiotic-resistant plasmids is established to increase 1.2-2.8 times for gonococcus and 1.2-2.7 for staphylococcus as compared to sensitive ones.
authors.affiliation: Dniepropetrovsk National University, 13 Naukovy Lane, Dniepropetrovsk, 49050, Ukraine.
authors.forename: T V
authors.initials: TV
authors.lastname: Skliar
authors.forename: A V
authors.initials: AV
authors.lastname: Krysenko
authors.forename: S N
authors.initials: SN
authors.lastname: Kozitskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Gavriliuk
authors.forename: A I
authors.initials: AI
authors.lastname: Vinnikov
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000201:enzymology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D004024:Dicyclohexylcarbodiimide; D000251:Adenosine Triphosphatases
vernacular_title: ATFaznaia aktivnost' shtammov Neisseria gonorrhoeae i Staphylococcus aureus.