title: Usefulness of Auto Cyto Fix (membrane filter method) for the application of immunohistochemistry.
abstract: We developed the Auto Cyto Fix (ACF) 1000 as an experimental model, which was adapted as a membrane filter method for automatically making smear preparations fixed with 95% ethanol. In this study, immunohistochemistry was applied to the cells smeared on the membrane filter, and effectiveness was examined. Twenty-four effusions and HeLa 229 cells were used. These cell samples were produced by the ACF1000 on the membrane filter and stained by the Papanicolaou method. After observation, these were decolored and stained by immunohistochemical techniques. Antibodies included CEA, EMA, and MIB-1. CEA and EMA were detected by the indirect method, and were colored by DAB, AEC, and new fuchsin. MIB-1 was detected by the immunoperoxidase (LSAB) and the immunofluorescence method. A uniformly stabilized immunoreaction was obtained and was equivalent to or better than that of the conventional technique. In addition, there was no background staining on the membrane filter. Based on these results, the membrane filter preparation produced by the ACF1000 was also effectively applied to immunohistochemical observations.
authors.affiliation: Department of Cytology, Kyorin University School of Health Sciences, Tokyo, Japan. iijimaj@kyorin-u.ac.jp
authors.forename: Junko
authors.initials: J
authors.lastname: Iijima
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Shiina
authors.forename: Mitsuaki
authors.initials: M
authors.lastname: Ohkoudo
authors.forename: Kanae
authors.initials: K
authors.lastname: Sakuma
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000556:secondary; D014408:Biomarkers, Tumor / Q000032:analysis; D003581:Cytodiagnosis / Q000295:instrumentation* / Q000379:methods; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation* / Q000379:methods; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology; D008851:Micropore Filters; D016066:Pleural Effusion, Malignant / Q000737:chemistry / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor