Transcriptional regulation of Nostoc hydrogenases: effects of oxygen, hydrogen, and nickel.
The transcription of structural genes encoding two hydrogenases in N(2)-fixing cultures of the cyanobacteria Nostoc muscorum and Nostoc sp. strain PCC 73102 were examined by reverse transcription-PCR. A low level of oxygen and addition of nickel induce higher transcript levels of both hydrogenases, whereas molecular hydrogen has a positive effect on the transcription of the genes encoding only the uptake hydrogenase.
Axelsson
Rikard
R
Department of Physiological Botany, EBC, Uppsala University, SE-752 36 Uppsala, Sweden. Rikard.Axelsson@ebc.uu.se
Lindblad
Peter
P
D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D006859:Hydrogen / Q000494:pharmacology; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism*; D009532:Nickel / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D009532:Nickel; D006859:Hydrogen; D006864:Hydrogenase; D010100:Oxygen