Biosynthetic requirements for the repair of membrane damage in pressure-treated Escherichia coli.
Cells of Escherichia coli that survived pressure treatment at 400 MPa showed increased sensitivity to sodium deoxycholate or sodium chloride in the plating medium, implying that homeostatic or barrier functions associated with outer and cytoplasmic membranes, respectively, were impaired. Repair of such sublethal membrane damage occurred when cells were incubated at 37 degrees C in tryptone soya broth. Inhibitor studies indicated that repair of cytoplasmic membrane damage was energy-dependent and required RNA and protein synthesis, whereas repair of outer membrane damage occurred with no requirement for energy or RNA or protein synthesis.
Chilton
P
P
Department of Chemistry, University of Reading, UK.
Isaacs
N S
NS
Manias
P
P
Mackey
B M
BM
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002462:Cell Membrane / Q000502:physiology*; D015169:Colony Count, Microbial; D003840:Deoxycholic Acid; D003902:Detergents; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D006874:Hydrostatic Pressure / Q000009:adverse effects; D012965:Sodium Chloride; D013997:Time Factors
D001426:Bacterial Proteins; D003902:Detergents; D003840:Deoxycholic Acid; D012965:Sodium Chloride