Cell cycle analysis by cyclin E + A/DNA multiparameter flow cytometry in exponential growth MOLT-4 cells.
OBJECTIVE
To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease.


METHODS
Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT-4 cells, and measured by flow cytometry.


RESULTS
We developed a cyclin E + A/DNA flow cytometry analysis method, which may distinguish G0, early G1, late G1, S, G2 and M phase cells, rather than three phases in the DNA content histogram.


CONCLUSIONS
Cyclin E + A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G0, early G1, late G1, S, G2 and M phase cells. It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation.
Qin
J
J
Surgery Department/Molecular Medical Centre, Tongji Hospital, Tongji Medical University, Wuhan 430030, China.
Tao
D
D
Shu
D
D
Leng
Y
Y
Gong
J
J
D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D019925:Cyclin A / Q000032:analysis*; D019927:Cyclin E / Q000032:analysis*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D014407:Tumor Cells, Cultured
D019925:Cyclin A; D019927:Cyclin E; D004247:DNA