[Study on the mechanism of thrombosis by lupus anticoagulant inhibited protein C pathway].
OBJECTIVE
To study the mechanism of thrombosis in systemic lupus erythematosus (SLE) patients with protein C (PC) pathway inhibition by lupus anticoagulant(LA).


METHODS
Phosphoethylamine(PE) dependent activated protein C (APC) inhibiting assay (modifed DRVVT) was used to observe the inhibition of APC activity after incubation of IgG from both normal and SLE patients with PE.


RESULTS
The activity of anticoagulation of APC was augmented by PE. Proportionally to its concentration, LA could inhibit the PE dependent anticoagulation of APC. The inhibition of LA-IgG on APC in the LA positive SLE patients with thrombosis was stronger than that in LA negative non-thrombosis patients; but IgG has no effect on the APC activity in normal person.


CONCLUSIONS
LA inhibiting the PC pathway by interfering with PE may be the important reason of thrombosis in SLE patients.
Wu
J
J
Anhui Provincial Hospital, Hefei 230001, China.
Zhou
Z
Z
Xu
J
J
D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism / Q000494:pharmacology; D016682:Lupus Coagulation Inhibitor / Q000378:metabolism* / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000150:complications*; D011486:Protein C / Q000819:agonists / Q000037:antagonists & inhibitors*; D013927:Thrombosis / Q000209:etiology* / Q000378:metabolism
D004983:Ethanolamines; D007074:Immunoglobulin G; D016682:Lupus Coagulation Inhibitor; D011486:Protein C; C005448:phosphorylethanolamine