TT virus: preferential distribution in CD19(+) peripheral blood mononuclear cells and lack of viral integration.
In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20% (25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19(+) cells. TTV DNA was also detected in CD3(+)/CD19(+) double-depleted cells but was undetectable in CD3(+) cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19(+) cells, CD3(+)-depleted, and CD3(+)/CD19(+) double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication.
Yu
Qigui
Q
Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, Hawaii, USA. qigui.yu@utoronto.ca
Shiramizu
Bruce
B
Nerurkar
Vivek R
VR
Hu
Ningjie
N
Shikuma
Cecilia M
CM
Melish
Marian E
ME
Cascio
Karin
K
Imrie
Allison
A
Lu
Yuanan
Y
Yanagihara
Richard
R
D000328:Adult; D018941:Antigens, CD19 / Q000378:metabolism*; D001483:Base Sequence; D017252:CD3 Complex / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D015995:Prevalence; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016662:Virus Integration; D014779:Virus Replication
D018941:Antigens, CD19; D017252:CD3 Complex; D004279:DNA, Viral