title: Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle.
abstract: OBJECTIVE
  To investigate the gene expression at transcription level of growth factor Wnt-5A in different phase during the cell cycle.
  
  
  METHODS
  We synchronized the renal cell carcinoma GRC-1 cell line by double thymidine blocks and high-pressure N2O gae methods and amplified Wnt-5A cDNAs from different phase using Semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction). The PCR products were electrophoresized on the agrose gel and detected by Gel Doc 1000 computer controlled system integrating the volumes of each band, representing the intensities of all pixels in a defined band.
  
  
  RESULTS
  The different mRNA expressions of growth factor Wnt-5A was detected in RCC GRC-1 cell line. In S phase, the highest level of Wnt-5A transcript was observed, and in G1 and M phase, medial and lowest, respectively. The differences between S and M stages were statistically significant (P < 0.05).
  
  
  CONCLUSIONS
  Growth factor Wnt-5A has the potential effect on tumorigenesis. It contributes to all phases during cell cycle but in S phase especially.
authors.affiliation: Urological Department, First Hospital, Institute of Urology, Beijing Medical University, Beijing 100034, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhuang
authors.forename: H
authors.initials: H
authors.lastname: Guo
authors.forename: D
authors.initials: D
authors.lastname: Xin
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Yuan
authors.forename: D
authors.initials: D
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Ding
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein