title: [The significance of CD3/MIB1 and CD3/CD25 in B cell lymphomas].
abstract: OBJECTIVE
  To identify the distribution and significance of proliferating T cells (PTC) in normal tonsil and a group of B cell lymphomas.
  
  
  METHODS
  Double immunohistochemical staining was used to detect PTC with MIB1 expression. Giemsa staining was used to detect Helicobacter pylori (Hp) in gastric MALT-type lymphoma. Cell culture was applied to investigate the relationship among tumor cells, Hp and T cells.
  
  
  RESULTS
  Very few T cells expressed MIB1 in the follicular centre of normal tonsil and in that of follicular lymphoma. PTC were mainly distributed to the extrafollicular and T cell zones. So did CD3+ T cells expressed CD25. In low grade primary B cell gastric MALT-type lymphomas, tumor cells proliferated in vitro in response to Hp but died in the absence of T cells. PTC increased with increase in the degree of malignancy in different kinds of B cell lymphomas.
  
  
  CONCLUSIONS
  T cells in follicular centres of follicular lymphoma are not in cell cycle. Proliferation of neoplastic B cells in low grade gastric MALT-type lymphomas requires Hp-specific infiltrating T cells. Transformation from low to high grade lymphoma is associated with an increase in T cell activation. T cells expressing MIB1 might be related to prognosis.
authors.affiliation: Department of Pathology, Shanxi Tumour Hospital, Taiyuan 030013, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
mesh_terms: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D009687:Nuclear Proteins; D015375:Receptors, Interleukin-2