Effects of endothelin-3 on intestinal ion transport.
We investigated the effects of endothelin 3 (ET-3) on electrolyte transport in rat small intestine using a voltage clamp technique in Ussing's chamber. ET-3 diminished potential difference (PD) and short circuit current (Isc). ET-3 did not affect PD or Isc in low Na(+) and/or D-glucose-free medium. Phloridzine (an inhibitor of sodium-glucose cotransporter [SGLT1]) pretreatment abolished the effect of ET-3 on Isc. Methylene blue (a soluble guanylate cyclase inhibitor) or N-nitro-L-arginine methyl ester (a NOS inhibitor) pretreatment delayed the effect of ET-3 on PD and Isc. ET-3 enhanced NOS activity on enterocytes and systemic NO production. Then, ET-3 could inhibit SGLT1 with the participation of NO.
González Bosc
L V
LV
Cátedra de Biología Celular e Histología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires. Junín 956, 1er piso (1113), Buenos Aires, Argentina. lgbosc@ffyb.uba.ar
Majowicz
M P
MP
Ortiz
M C
MC
Vidal
N A
NA
D000818:Animals; D019334:Endothelin-3 / Q000494:pharmacology*; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D017136:Ion Transport; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009566:Nitrates / Q000652:urine; D009569:Nitric Oxide / Q000502:physiology; D009573:Nitrites / Q000652:urine; D051381:Rats; D017208:Rats, Wistar
D019334:Endothelin-3; D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide; D009252:NADPH Dehydrogenase