[Influence of focal adhesion kinase (FAK) on the biological behaviors of human hepatocellular carcinoma cells].
OBJECTIVE
To study the effect of reducing FAK expression on the malignant biological behaviors of FAK-overexpressed hepatocellular carcinoma cell line SMMC-7721.


METHODS
FAK expression of different hepatocelluar carcinoma cell lines was detected by Western blot analysis. Antisense plasmid of FAK was constructed and transfected into SMMC-7721.


RESULTS
SMMC-7721 expressed much more FAK than did hepatocyte line L02. When transfected with autisense FAK, it showed reduced growth rate and colony formation in soft agar, and the number of cells in S phase was reduced by 15%. The adhesion ability of antisense FAK-transfeected cells was also decreased with no change of integrin expression.


CONCLUSIONS
FAK is overexpressed in the human hepatocellular carcinoma cell line SMMC-7721. Reducing the FAK expression can partly reverse the malignant behaviors of the cell line.
Fang
Y
Y
Department of Biochemistry, Ministry of Public Health Key Laboratory of Glycoconjugate Research, Shanghai Medical University, Shanghai 200032, China.
Fang
X
X
Wang
L
L
D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human