Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment.
A novel DNA assay demonstrating sensitive and accurate detection of Helicobacter pylori from stool samples is reported. Moreover, in three individuals tested for therapeutic response, the assay showed the disappearance of H. pylori DNA during treatment. Thus, this noninvasive molecular biology-based assay has the potential to be a powerful diagnostic tool given its ability to specifically identify H. pylori DNA.
Shuber
Anthony P
AP
Applied Research Group, Exact Sciences Corporation, Maynard, Massachusetts 01754, USA.
Ascaño
Jennifer J
JJ
Boynton
Kevin A
KA
Mitchell
Anastasia
A
Frierson
Henry F
HF
El-Rifai
Wa'el
W
Powell
Steven M
SM
D004269:DNA, Bacterial / Q000032:analysis*; D005243:Feces / Q000382:microbiology*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial