The presence of 9-cis-beta-carotene in cytochrome b(6)f complex from spinach.
Cytochrome b(6)f complex with stoichiometrically bound beta-carotene molecule was purified from spinach chloroplasts. The configuration of this beta-carotene was studied by reversed-phase HPLC and resonance Raman spectroscopy. Both the absorption spectrum of this beta-carotene in dissociated state and the Raman spectrum in native state can be unambiguously assigned to a 9-cis configuration. This finding is in contrast to the predominantly all-trans isomers commonly found in membranes and protein-pigment complexes of chloroplasts, suggesting that the 9-cis-beta-carotene is an authentic component and may have a unique structural and functional role in cytochrome b(6)f complex.
Yan
J
J
Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing, PR China.
Liu
Y
Y
Mao
D
D
Li
L
L
Kuang
T
T
D002851:Chromatography, High Pressure Liquid; D003573:Cytochrome b Group / Q000737:chemistry / Q000302:isolation & purification*; D045346:Cytochrome b6f Complex; D013059:Spectrum Analysis, Raman; D018724:Spinacia oleracea / Q000737:chemistry*; D019207:beta Carotene / Q000737:chemistry*
D003573:Cytochrome b Group; D019207:beta Carotene; D045346:Cytochrome b6f Complex