Preliminary assessment of the immune response to Olea europaea pollen extracts encapsulated into PLGA microspheres.
In this study, poly (D,L-lactide-co-glycolide) (PLGA) microspheres encapsulating Olea europaea pollen extracts were prepared by using the double emulsion (w/o/w) based on a solvent evaporation/extraction method. The resulting microspheres were 1.93 microns in size. The total allergen loading and surface-associated allergen were 8 and 0.64%, respectively. The release of the allergen from the microspheres showed a biphasic profile with an initial burst release followed by a sustained release phase. Finally, the polyacrylamide gel electrophoresis (SDS-PAGE) results showed that the encapsulation process does not affect the stability of the protein. We describe here some preliminary observations concerning the use of these microspheres as parenteral antigen delivery systems for immunization with O. europaea pollen extracts, in a small animal model, the mouse.
Igartua
M
M
Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country (UPV-EHU), Paseo de la Universidad no. 7, 01006 Vitoria-Gasteiz, Spain.
Hernández
R M
RM
Gutierro
I
I
Gascón
A R
AR
Pedraz
J L
JL
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D003888:Desensitization, Immunologic / Q000379:methods*; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007279:Injections, Subcutaneous; D019344:Lactic Acid / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D029592:Oleaceae / Q000276:immunology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011100:Polyglycolic Acid / Q000008:administration & dosage; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage
D001672:Biocompatible Materials; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010936:Plant Extracts; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid