Cloning and characterisation of the Hint homologue of the thermophile Thermus thermophilus.
Screening of a genomic library of the thermophile Thermus thermophilus revealed a novel thermophilic hint gene, homologues of which are highly conserved in genera from archaea to mammals. Hint belongs to the HIT protein super-family, which contains two broad groups, Fhit, associated with tumour suppression in eukaryotes and Hint with putatitively protein kinase C inhibitory activity. In T. thermophilus the 321 bp gene has a GC content of 67% overall and 94.4% in the third nucleotide position, with unusually no thymine as a wobble base. The gene product, a small highly conserved 11,996 Da predicted soluble cytoplasmic protein, offers an ideal opportunity to investigate thermostabilising amino acid substitutions. Here we report on the characterisation of the novel hint sequence.
Gibert
Y
Y
Industrial Biochemistry Group, Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland.
Spada
S
S
Wall
J G
JG
Pembroke
J T
JT
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016963:Thermus thermophilus / Q000235:genetics*
C405403:Hinterteil protein, Hydra vulgaris; D011505:Protein-Tyrosine Kinases