Placental transfer of the estrogenic mycotoxin zearalenone in rats.
In order to study the possible placental transfer of the Fusarium mycotoxin zearalenone (ZON), Sprague Dawley rats were treated with a single dose (0.74 mg/kg b.w.) of ZON i.v. on day 12 or day 18 of pregnancy, or intragastrically (i.g.) on day 18 of pregnancy. Samples of placenta, foetus, and maternal liver and spleen were collected for chemical analyses 0.3 h after treatment on day 12, and 0.3, 4, and 24 h after treatment on day 18. Three rats were used for each pregnancy day, administration route, and exposure time. The concentrations of ZON and its metabolites alpha- and beta-zearalenol (-ZOL) were determined quantitatively by high-performance liquid chromatography (HPLC) after incubation with beta-glucuronidase and purification on immunoaffinity columns. Tissue distribution was studied by means of whole body autoradiography at 4 and 24 h after treatment with tritiated ZON (750 microCi/kg b.w; 7.4 mg/kg b.w.) on day 18 of pregnancy. ZON and alpha-ZOL were transferred into the foetus on both gestational days. However, a delay in distribution into the foetus, relative to the maternal tissue, was observed. Beta-ZOL was below the detection limit in the foetus. No specific site of foetal accumulation of ZON or its metabolites was apparent. In the maternal tissues, the highest levels of ZON and of alpha- and beta-ZOL were found in the liver.
Bernhoft
A
A
Department of Food and Feed Hygiene, National Veterinary Institute, Oslo, Norway. aksel.bernhoft@vetinst.no
Behrens
G H
GH
Ingebrigtsen
K
K
Langseth
W
W
Berndt
S
S
Haugen
T B
TB
Grotmol
T
T
D000284:Administration, Oral; D000818:Animals; D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D004968:Estrogens, Non-Steroidal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D007275:Injections, Intravenous; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014018:Tissue Distribution; D014316:Tritium; D015025:Zearalenone / Q000008:administration & dosage / Q000493:pharmacokinetics*
D004968:Estrogens, Non-Steroidal; D014316:Tritium; D015025:Zearalenone