Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting.
The cell line B95-8 releases Epstein-Barr virus (EBV) with high titres of transforming activity and is widely used as a model in cancer research and virology. There are, however, controversial reports about the species of origin, cell line stability and karyotype. To address these questions, B95-8 chromosomes were analysed by chromosome sorting and painting by multicolour fluorescence in-situ hybridization. Reciprocal painting was performed between B95-8, 'wildtype' New World monkey and human chromosomes. Saguinus oedipus was revealed as the species of origin. A further five cell-line-specific marker chromosomes, resulting from translocations, deletions and an insertion were found. Although human chromosome 6 or 13 homologues were always involved in these rearrangements, co-hybridization of an EBV-specific DNA probe did not reveal site-specific hybridization to marker chromosomes or at translocation breakpoints. The multicolour probe set described here will be of special value for further evolutionary studies in New World monkeys.
Müller
S
S
Institute of Anthropology and Human Genetics, University of Munich (LMU), Germany.
Neusser
M
M
O'Brien
P C
PC
Wienberg
J
J
D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D020223:Chromosome Painting; D002875:Chromosomes; D002882:Chromosomes, Human, Pair 13; D002896:Chromosomes, Human, Pair 6; D020732:Cytogenetic Analysis; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D012452:Saguinus / Q000235:genetics*; D013045:Species Specificity
D004279:DNA, Viral