title: Dengue virus replicative intermediate RNA detection by reverse transcription-PCR.
abstract: Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.
authors.affiliation: Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Carpio 470, Col. Santo Tomas, México DF 11340, México.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vaughan
authors.forename: Hiram
authors.initials: H
authors.lastname: Olivera
authors.forename: Leopoldo
authors.initials: L
authors.lastname: Santos-Argumedo
authors.forename: Abraham
authors.initials: A
authors.lastname: Landa
authors.forename: Baltasar
authors.initials: B
authors.lastname: Briseño
authors.forename: Alejandro
authors.initials: A
authors.lastname: Escobar-Gutiérrez
mesh_terms: D000818:Animals; D002460:Cell Line; D009033:Culicidae; D003716:Dengue Virus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: D012367:RNA, Viral