Antigenicity of poly(I) and ROS-poly(I) and their recognition of human anti-DNA autoantibodies.
The effect of hydroxyl radicals on polyinosinic acid [poly(I)] was studied. Strand breaks, base alteration and a decrease in absorbance at 248 nm (lambda max) were observed upon *OH modification of poly(I). The broad antigen specificity of the induced anti-poly(I) and anti-ROS-poly(I) antibodies showed diverse antigen binding characteristics similar to those of SLE autoantibodies. Recognition of both poly(I) and ROS-poly(I) by human SLE anti-DNA autoantibodies was observed. The possible significance of these findings in the etiology of SLE has been discussed.
Tasneem
S
S
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Ali
R
R
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D011069:Poly I / Q000276:immunology*; D011817:Rabbits
D000974:Antibodies, Antinuclear; D011069:Poly I; D006861:Hydrogen Peroxide