title: Contribution of Hsc70 to barotolerance in the yeast Saccharomyces cerevisiae.
abstract: The contribution of Hsc70 to barotolerance in logarithmic-phase cells of the HSC70 (ssb1 and ssb2) deletion mutant and in strains expressing the HSC70 gene on either a low- or a high-copy-number plasmid was studied. The deletion-mutant strain had higher thermotolerance and a slightly lower barotolerance than the control strain. The strain that expresses the HSC70 gene in high copy number had a higher barotolerance than the strain that expresses the gene in low copy number. These results suggest that Hsc70 contributes to barotolerance during exponentially growing conditions as does Hsp104 during heat-shock treatment.
authors.affiliation: National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki, Japan. hitoshi.iwahashi@aist.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Iwahashi
authors.forename: S
authors.initials: S
authors.lastname: Nwaka
authors.forename: K
authors.initials: K
authors.lastname: Obuchi
mesh_terms: D017353:Gene Deletion; D005800:Genes, Fungal; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006874:Hydrostatic Pressure; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014199:Trehalose / Q000378:metabolism
chemical_list: D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D014199:Trehalose