[Muscle pigment epithelium-derived factor gene associating with tumorigenesis of B16 melanoma].
OBJECTIVE
Random amplified polymorphic DNA (RAPD) analysis was used to detect the genomic variant in B16 melanoma, and to seek the tumor related DNA fragments.


METHODS
Genomic DNA from B16 melanoma and C57BL/6J mouse normal tissues were amplified by RAPD with 105 random primers, the significantly different DNA fragments were isolated, cloned and sequenced. DNA sequences were analyzed with GenBank data.


RESULTS
24 of the 105 primers generated polymorphic profiles when the B16 melanoma RAPD profile was compared to that of its normal tissue DNA. By amplification of the primer AB8-5, a tumor band showing loss with respect to normal band was detected and this DNA fragment was designated as B8-5. B8-5 was a 610 bp fragment, the sequencing result of B8-5 showed 99% homology with muscle pigment epithelium-derived factor (PEDF) gene, and 100% homology with muscle pigment epithelium-derived factor mRNA. B8-5 was therefore regarded as PEDF gene.


CONCLUSIONS
Our result found that allelic losses exist in PEDF gene, and therefore suggest that PEDF is associated with tumorigenesis of B16 melanoma.
Li
S
S
Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Chen
Y
Y
Wei
H
H
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015197:Carcinogenicity Tests; D004273:DNA, Neoplasm / Q000032:analysis*; D005136:Eye Proteins; D008546:Melanoma, Experimental / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D019105:Random Amplified Polymorphic DNA Technique; D015843:Serpins / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured; D000099174:Pigment Epithelium-Derived Factor
D004273:DNA, Neoplasm; D005136:Eye Proteins; D009414:Nerve Growth Factors; D011506:Proteins; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor