title: Allelic loss analysis by capillary electrophoresis: an accurate, automated method for detection of deletions in solid tumors.
abstract: In this report, we describe a fast and accurate capillary electrophoresis, PCR-based method for detecting loss of allelic heterozygosity in solid tumor samples. This automated method requires small sample sizes, and data can be obtained in less than 15 min. The method is particularly powerful for uncovering deletions in tumor sample preparations containing significant normal tissue contamination.
authors.affiliation: Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA. sells@uab.edu
authors.forename: S M
authors.initials: SM
authors.lastname: Sell
authors.forename: S
authors.initials: S
authors.lastname: Patel
authors.forename: D
authors.initials: D
authors.lastname: Stracner
authors.forename: A
authors.initials: A
authors.lastname: Meloni
mesh_terms: D002897:Chromosomes, Human, Pair 7; D004273:DNA, Neoplasm; D019075:Electrophoresis, Capillary; D017353:Gene Deletion; D006801:Humans; D007889:Leiomyoma / Q000235:genetics; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D009396:Wilms Tumor / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm