Blue-light-dependent osmoregulation in protoplasts of Phaseolus vulgaris Pulvini.
Blue light was found to induce shrinkage of the protoplasts isolated from first-leaf lamina pulvini of 18-day-old Phaseolus vulgaris. The response was transient following pulse stimulation, while it was sustainable during continuous stimulation. No apparent difference was found between flexor and extensor protoplasts. Protoplasts of the petiolar segment located close to the pulvinus showed no detectable response. In the plants used, the pulvinus was fully matured and the petiole was ceasing its elongation growth. When younger, 12-day-old, plants were used, however, the petiolar protoplasts did respond to blue light. The pulse-induced response was similar to that in pulvinar protoplasts, although the response to continuous stimulation was transient and differed from that in pulvinar protoplasts. No shrinkage was induced in pulvinar protoplasts when the far-red-light-absorbing form of phytochrome was absent for a period before blue-light stimulation, indicating that the blue-light responsiveness is strictly controlled by phytochrome. Inhibitors of anion channels and H(+)-ATPase abolished the shrinking response, supporting the view that protoplasts shrink by extruding ions. The response of pulvinar protoplasts is probably involved in the blue-light-induced, turgor-based movement of pulvini. The blue-light responding system in pulvini is suggested to have evolved from that functioning in other growing organs.
Wang
X
X
Botanical Gardens, Graduate School of Science, Osaka City University, Kisaichi, Katano-shi, Osaka, 576-0004 Japan.
Haga
K
K
Nishizaki
Y
Y
Iino
M
M
D000222:Adaptation, Physiological; D002118:Calcium / Q000378:metabolism; D048429:Cell Size; D066298:In Vitro Techniques; D007473:Ion Channels / Q000037:antagonists & inhibitors; D007700:Kinetics; D008027:Light; D009579:Nitrobenzoates / Q000494:pharmacology; D009997:Osmotic Pressure; D027805:Phaseolus / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism / Q000528:radiation effects; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D018516:Pulvinus / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology; D014867:Water / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000528:radiation effects
D007473:Ion Channels; D009579:Nitrobenzoates; D014867:Water; D010834:Phytochrome; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D014638:Vanadates; D006180:Proton-Translocating ATPases; D002118:Calcium