Biomaterials for molecular electronics development of optical biosensor for retinol.
Molecular electronics involves expertise from several branches of science. Various biomaterials and electronics are involved in the fabrication of such devices. While passive biomaterials are involved in anchoring the active biomolecules, the latter are involved in switching and/or signal transduction. In the present investigation we have used a glass-capillary-based approach to design a biosensor for retinol. The sensing element is retinol-binding protein (RBP). The affinity of retinoic-acid-horseradish peroxidase (conjugate) to RBP is tested using a surface plasmon resonance technique. A simple photomultiplier-tube-based system is exploited to monitor the chemiluminescent signal generated upon reaction of hydrogen peroxide and luminol with the conjugate bound to RBP. The photomultiplier tube is directly coupled to a computer for data logging.
Ramanathan
K
K
Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. alamelu6703@hotmail.com
Svitel
J
J
Dzgoev
A
A
Sundaram
P V
PV
Danielsson
B
B
D001672:Biocompatible Materials; D015374:Biosensing Techniques; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D004581:Electronics; D005898:Glass; D006735:Horseradish Peroxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D055095:Optics and Photonics; D012177:Retinol-Binding Proteins / Q000737:chemistry; D020349:Surface Plasmon Resonance; D013997:Time Factors; D014212:Tretinoin / Q000737:chemistry; D014801:Vitamin A / Q000737:chemistry*
D001672:Biocompatible Materials; D012177:Retinol-Binding Proteins; D014801:Vitamin A; D014212:Tretinoin; D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase