[Effect of adenovirus type 5 E1A gene on proliferation and metastasis of human lung adenocarcinoma cells].
OBJECTIVE
To investigate the effects of E1A gene on proliferation and metastasis of human lung adenocarcinoma cells.


METHODS
A mammalian E1A-expressing recombinant constructed in our laboratory, named pcDNA3-E1A, was introduced into human lung adenocarcinoma cell line(Anip 973) by lipofectamine. The cells resistant to G418 were selected. The characteristics of the E1A-expressing Anip 973(Anip 973-E1A) cells were studied in vitro and in vivo, including cell growth rate and doubling-time, colony-forming efficiency on soft agarose, tumorigenicity and metastasis.


RESULTS
The growth rate of E1A-Anip 973 cells was diminished and their colony-forming efficiency was inhibited significantly. The tumorigenicity in nude mice of the E1A-expressing Anip 973 was markedly suppressed.


CONCLUSIONS
E1A suppresses the malignant phenotype of human lung adenocarcinoma cell line. This study provides experimental data for gene therapy of lung cancer with E1A.
Qian
X
X
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
Zhao
Q
Q
Wang
Z
Z
D000230:Adenocarcinoma / Q000556:secondary; D017500:Adenovirus E1A Proteins / Q000235:genetics / Q000627:therapeutic use*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000517:prevention & control*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D017500:Adenovirus E1A Proteins