title: Recording of spared motor evoked potentials and its augmentation by 4-aminopyridine in chronic spinal cord-injured rats.
abstract: OBJECTIVE
  To research the direct electrophysiological evidence of discomplete spinal cord injury (SCI) and the effect of 4-aminopyridine on it.
  
  
  METHODS
  Motor evoked potentials (MEPs), both spinal cord recorded MEPs (scMEPs) and extracellularly recorded MEPs (exMEPs) were recorded and characterized on a T13 epidural electrode (scMEPs) and an extracellular microelectrode (exMEPs) for 10 normal rats and 40 rats with lesions of various severity (sham, 35 g.cm force (gcf), 70 gcf, 100 gcf impact injury) at the T8-T9 cord using the Allen's drop model. The incline plane and Tarlov techniques were used to assess clinical neurological function.
  
  
  RESULTS
  MEPs in the normal rats were elicited by applying transcortical suprathreshold stimulation consisting of 3-4 early negative peaks (N1, N2, N3 and N4) followed by several late waves. The N1 and N2 peaks were largest in the anterior and ventrolateral funiculus, respectively, which was indicative of extrapyramidal pathways. The 100 gcf impact injuries and the cord transection abolished the MEP distal to the lesion, whereas the 35 gcf injuries resulted in a latency shift and amplitude decrement of the MEP peaks. Eighteen of the 20 rats with 70 gcf-injuries showed clinical paraplegia. Among them, 7 rats had neurophysiological evidence of residual conduction pathways through the lesioned cord segment, such as the presence of N1 and N2 peaks in the scMEPs or exMEPs. After 4-aminopyridine (4-AP) administrations (1 mg/kg), the amplitude of the spared exMEP increased significantly and spread more widely.
  
  
  CONCLUSIONS
  MEPs evoked by transcortical stimulation travel mostly in the extrapyramidal tract. MEP monitoring could provide an excellent method of detecting the functional integrity of the motor tracts after SCI, and could even detect spared motor fibers after discomplete SCI. Furthermore, the use of 4-AP or other K+ channel blocking agents may be a potential treatment for patients with chronic moderate to severe SCI.
authors.affiliation: Department of Orthopedics, Shanghai Municipal Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China. kwyu@sh163b.sta.net.cn
authors.forename: K
authors.initials: K
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Rong
authors.forename: L
authors.initials: L
authors.lastname: Jia
authors.forename: W
authors.initials: W
authors.lastname: Yuan
authors.forename: X
authors.initials: X
authors.lastname: Ye
authors.forename: Z
authors.initials: Z
authors.lastname: Shi
authors.forename: B
authors.initials: B
authors.lastname: Dai
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology*; D000818:Animals; D004558:Electric Stimulation; D019054:Evoked Potentials, Motor / Q000187:drug effects* / Q000502:physiology; D008297:Male; D026902:Potassium Channel Blockers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D026902:Potassium Channel Blockers; D015761:4-Aminopyridine