Egg sialoglycans increase intracellular pH and potentiate the acrosome reaction of sea urchin sperm.
Sea urchin egg jelly (EJ) triggers sperm acrosome reaction (AR), an exocytotic event required for membrane fusion of the gametes. Purified fucose sulfate polymer (FSP) in EJ is one inducer of the AR. Binding of FSP to its receptor regulates opening of two distinct calcium channels and also elevates intracellular pH (pH(i)). EJ also contains sialic acid-rich glycans (sialoglycans (SG)) that were isolated by beta-elimination followed by DEAE chromatography. In the presence of limiting amounts of FSP, the SG fraction markedly potentiates the AR; however, by itself SG has no activity. The SG fraction increases the pH(i) of sperm without increasing intracellular Ca(2+). The SG-induced increase in pH(i) is not blocked by nifedipine or high K(+), whereas the FSP-induced pH(i) increase is sensitive to both these agents. Treatment of the SG fraction with neuraminidase or mild metaperiodate that specifically cleaves the glycerol side chain of sialic acid abolishes the AR potentiation and ability of SG to elevate pH(i). These data are the first to show that there are at least two pathways to induce sperm pH(i) increase and that egg surface sialic acid plays a role in triggering the sperm AR.
Hirohashi
Noritaka
N
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0202, USA. nhirohashi@ucsd.edu
Vacquier
Victor D
VD
D020101:Acrosome Reaction; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002845:Chromatography; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004983:Ethanolamines / Q000494:pharmacology; D006601:Hexoses / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D012617:Sea Urchins; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D013997:Time Factors
D003432:Cross-Linking Reagents; D004983:Ethanolamines; D006601:Hexoses; D011134:Polysaccharides; D012795:Sialoglycoproteins; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase; D011188:Potassium; C007369:2-diethylaminoethanol; D010100:Oxygen; D002118:Calcium