Sitosterol-beta-glucoside as primer for cellulose synthesis in plants.
Cellulose synthesis in plants requires beta-1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-beta-glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5'-diphosphate-glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The cellulase encoded by the Korrigan (Kor) gene, required for cellulose synthesis in plants, may function to cleave SG from the growing polymer chain.
Peng
Liangcai
L
Section of Plant Biology, University of California Davis, One Shields Avenue, Davis, CA 95616, USA.
Kawagoe
Yasushi
Y
Hogan
Pat
P
Delmer
Deborah
D
D029681:Arabidopsis Proteins; D002118:Calcium / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D003912:Dextrins / Q000096:biosynthesis; D004533:Egtazic Acid / Q000494:pharmacology; D005936:Glucans / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D003368:Gossypium / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D012855:Sitosterols / Q000378:metabolism*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D003912:Dextrins; D005936:Glucans; D006540:Herbicides; C429844:KOR1 protein, Arabidopsis; D008565:Membrane Proteins; D009570:Nitriles; D011994:Recombinant Proteins; D012855:Sitosterols; D002475:Cellobiose; D004533:Egtazic Acid; C025473:gamma-sitosterol; D002482:Cellulose; C068994:cellodextrin; C048306:callose; D005964:Glucosyltransferases; C457769:PRC1 protein, Arabidopsis; C028634:cellulose synthase (UDP-forming); D002480:Cellulase; D005947:Glucose; C055869:dichlobanil; D002118:Calcium; C011015:lyoniside; D014532:Uridine Diphosphate Glucose