title: Analysis of the 5S RNA pool in Arabidopsis thaliana: RNAs are heterogeneous and only two of the genomic 5S loci produce mature 5S RNA.
abstract: One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5'- and 3'-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.
authors.affiliation: U.M.R. 6547 BIOMOVE, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Cloix
authors.forename: Sylvie
authors.initials: S
authors.lastname: Tutois
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yukawa
authors.forename: Olivier
authors.initials: O
authors.lastname: Mathieu
authors.forename: Claudine
authors.initials: C
authors.lastname: Cuvillier
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Espagnol
authors.forename: Georges
authors.initials: G
authors.lastname: Picard
authors.forename: Sylvette
authors.initials: S
authors.lastname: Tourmente
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012332:RNA, Heterogeneous Nuclear; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S