DNA microarray analysis of gene expression markers of endometriosis.
OBJECTIVE
To use DNA microarray technology to examine differential gene expression in uterine endometrium versus endometriosis implants.


METHODS
Pilot study.


METHODS
Volunteers in an academic research environment.


METHODS
Premenopausal women scheduled for surgery for suspected endometriosis.


METHODS
Surgical excision of endometriosis tissue and uterine endometrial biopsy.


METHODS
Gene expression.


RESULTS
The expression of eight genes from a total of 4,133 genes on the DNA microarray was increased in endometriosis implants compared with uterine endometrium. The eight genes were beta-actin, alpha-2 actin, vimentin, 40S ribosomal protein S23, Ig-lambda light chain, Ig germline H chain G-E-A region gamma-2 constant region gene, major histocompatibility complex class 1,C, and complement component 1 S subcomponent.


CONCLUSIONS
The data demonstrate that the DNA microarray is an effective tool for the identification of differentially expressed genes between uterine and ectopic endometrium; further study of the genes identified herein will expand our understanding of the nature of endometriosis and assist in the eventual development of new treatments for endometriosis.
Eyster
Kathleen M
KM
Division of Basic Biomedical Sciences, University of South Dakota School of Medicine, Vermillion, South Dakota 57069-2390, USA. keyster@usd.edu
Boles
Amy L
AL
Brannian
John D
JD
Hansen
Keith A
KA
D000199:Actins / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D004715:Endometriosis / Q000235:genetics* / Q000601:surgery; D005260:Female; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006801:Humans; D008285:Major Histocompatibility Complex; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D017698:Postmenopause; D014746:Vimentin / Q000235:genetics
D000199:Actins; D003598:Cytoskeletal Proteins; D005819:Genetic Markers; D014746:Vimentin