title: Marginal leakage of filled dentin adhesives used with wet and dry bonding techniques.
abstract: OBJECTIVE
  To evaluate the effect of wet and dry bonding on microleakage of Class V restorations bonded with three filled bonding agents.
  
  
  METHODS
  Sixty extracted human premolar and molar teeth were randomly assigned to five groups for bonding with OptiBond Solo, PermaQuick PQI, Prime & Bond NT (one coat), Prime & Bond NT (two coats) and Prime & Bond 2.1, as a control material. Cavities were cut in both the buccal and lingual surfaces. Half of each preparation was in enamel and the other was in cementum/dentin. The cavities were restored with resin-based composite after the application of dentin bonding agents using a wet and dry technique for each material. The teeth were stored in distilled water for 6 days at 37 degrees C, thermocycled, and the restorations examined microscopically for leakage using Procion Brilliant Red as a marker.
  
  
  RESULTS
  All groups showed microleakage at both the enamel and dentin margins. At the gingival margin, there was a significant difference between the groups for wet bonding (P = 0.0349) but not for dry bonding (P = 0.9983) (Kruskal-Wallis test). There was no significant difference between the groups at the enamel margin (wet bonding: P = 0.9999, dry-bonding: P = 0.9304) (Kruskal-Wallis test). The wet-bonding technique was compared with the dry-bonding technique for each material at both the gingival and enamel margins and no significant differences were observed (in all cases P > 0.5) (Mann-Whitney U-test).
authors.affiliation: Department of Conservative Dentistry, University of Edinburgh, Scotland, UK.
authors.forename: A
authors.initials: A
authors.lastname: Santini
authors.forename: A J
authors.initials: AJ
authors.lastname: Plasschaert
authors.forename: S
authors.initials: S
authors.lastname: Mitchell
mesh_terms: D000704:Analysis of Variance; D001641:Bicuspid; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D004396:Coloring Agents; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D003737:Dental Cavity Preparation / Q000145:classification; D003739:Dental Cementum / Q000648:ultrastructure; D003743:Dental Enamel / Q000648:ultrastructure; D003763:Dental Leakage / Q000145:classification*; D018772:Dental Marginal Adaptation; D003793:Dental Restoration, Permanent / Q000145:classification; D003804:Dentin / Q000648:ultrastructure; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008963:Molar; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D018709:Statistics, Nonparametric; D013499:Surface Properties; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014227:Triazines; D014867:Water / Q000737:chemistry
chemical_list: D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C428805:OptiBond SOLO; C119199:PermaQuik; D011109:Polymethacrylic Acids; C103500:Prime & Bond; C425843:Prime and Bond NT; D019279:Resin Cements; D014227:Triazines; D014867:Water; C026429:Procion Brilliant Red H8-BS; D017438:Bisphenol A-Glycidyl Methacrylate