[The expression of human telomerase reverse transcriptase gene in cord blood hematopoietic stem cells and its significance].
OBJECTIVE
To investigate the expression and significance of human telomerase reverse transcriptase (hTERT) gene in cord blood hematopoietic stem cells.


METHODS
Using in situ hybridization techniques, we detect the expression of hTERT gene in cord blood hematopoietic stem cells in different condition during different culture time.


RESULTS
hTERT gene was lowly expressed in freshly isolated cord blood CD34+ cells; the positive rate was 13%. It could be increased in 5-7 days when the cells were cultured in vitro, especially in the presence of stem cell factor, interleukin-3(IL-3), IL-6 and Flt-3 Ligand together; the positive rate reached 48%. Transform growth factor-beta 1 and all-trans retinoic acid could repress hTERT gene.


CONCLUSIONS
hTERT gene was lowly expressed in the cord blood CD34+ cells; it could be upregulated in the culture in vitro along with optimal cytokines, but was downregulated by negative regulator and induction differentiation.
Ma
Y
Y
Department of Hematology, Union Hospital, Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430022, China.
Zou
P
P
D018952:Antigens, CD34 / Q000097:blood; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008565:Membrane Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology; D019098:Telomerase / Q000097:blood / Q000235:genetics*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D014212:Tretinoin / Q000494:pharmacology
D018952:Antigens, CD34; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D007377:Interleukin-3; D015850:Interleukin-6; D008565:Membrane Proteins; D019089:Stem Cell Factor; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C084881:flt3 ligand protein; D014212:Tretinoin; D019098:Telomerase