Overview and application of suspension array technology.
A cell-based solid-phase immunoassay usually is called immunophenotyping when performed on a flow cytometer. Using the same principles, there is a new flow cytometric application available with the suspension array technology. The significant difference is that the immunologic reaction does not occur on the surface of leukocytes, but rather on the surface of plastic fluorospheres. This is a multiplexed solid-phase flow cytometry-based technology with some clinically relevant potential.
Mandy
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National Laboratory for HIV Immunology, Health Canada, Ottawa, Ontario, Canada. Frank Mandy@HC-SC.GC.CA
Nakamura
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Bergeron
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Sekiguchi
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D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D007834:Lasers; D013677:Medical Laboratory Science / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity