[Relationship between blast crisis of chronic myeloid leukemias and abnormality of p16 and calcitonin genes].
OBJECTIVE
To study the relationship between homozygous deletions of p16 gene and calcitonin hypermethylation and chronic myeloid leukemia (CML) blast crisis.


METHODS
Semiquantitative multiplex polymerase chain reaction (PCR) was used to detect homozygous deletions of p16 gene and semiquantitative PCR to detect calcitonin gene hypermethylation in 53 CML patients.


RESULTS
In 53 CML patients, homozygous deletion of p16 gene was found in none of them in chronic phase, while in 6.3% (1/16) of them in myeloid blast crisis, 66.7% (8/12) in lymphoid blast crisis, and 2/5 (40%) in mixed cell blast crisis. Calcitonin gene hypermethylation was found in 10% (2/20), 68.8%(11/16), 16.7%(2/12), and 40% (2/5), respectively.


CONCLUSIONS
There is intimate relationship between homozygous deletions of p16 gene and CML in lymphoid blast crisis, between calcitonin gene hypermethylation and CML in myeloid blast crisis. Moreover, abnormality of both genes may be associated with mixed cell blast crisis in CML patients. Simultaneous detection of the two genes help early pickup CML patients in blast crisis.
Xu
D
D
Department of Hematology, First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China.
Hong
W
W
Wang
S
S
D000328:Adult; D002116:Calcitonin / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D017353:Gene Deletion; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction
D019941:Cyclin-Dependent Kinase Inhibitor p16; D005819:Genetic Markers; D002116:Calcitonin