cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: comparison with the acatalasemic mutant.
Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.
Wang
D H
DH
Department of Public Health, Okayama Graduate School of Medicine and Dentistry, Japan.
Tsutsui
K
K
Sano
K
K
Masuoka
N
N
Kira
S
S
D020642:Acatalasia / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D012313:RNA / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D011994:Recombinant Proteins; D012313:RNA; D002374:Catalase