[Effect of composite blood-activating decoction on expression of adherent molecule and cyclin in bone marrow hematopoietic cells in mice of immune-induced aplastic anemia].
OBJECTIVE
To study the effect of composite blood-activating decoction (CBAD) on expression of adherent molecule CD49 d and cyclin D2 in bone marrow hematopoietic cells in experimental immune-mediated aplastic anemia (AA) mice.


METHODS
After the model of AA was established, the animals were fed with 0.2 ml of 100% CBAD, twice a day for 12 days. Te CD49 d and cyclin D2 expression level of bone marrow hematopoietic cells in model mice were measured by flow cytometor analysis system at the 13th day of experiment.


RESULTS
CD49 d expression level in CBAD group was significantly higher than that in the AA group (P < 0.05), and was similar to that in the normal group (P > 0.05). The expression of cyclin D2 in CBAD group was significantly higher than that in the AA group (P < 0.01), but the cell count of G0 + G1 phase was significantly lower in the CBAD group, as compared with the AA group, P < 0.01.


CONCLUSIONS
CBAD can increase the expression of adherent molecule CD49 d and cyclin D2 in bone marrow hematopoietic cells, and promote the growth of hematopoietic cells.
Sun
H
H
Tongji Hospital, Tongji Medical University, Wuhan (430030).
Dong
L
L
Liu
W
W
D000741:Anemia, Aplastic / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D056742:Cyclin D2; D016213:Cyclins / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D039441:Integrin alpha4; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D011897:Random Allocation
D015703:Antigens, CD; C486196:Ccnd2 protein, mouse; D015815:Cell Adhesion Molecules; D056742:Cyclin D2; D016213:Cyclins; D004365:Drugs, Chinese Herbal; D039441:Integrin alpha4