Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin.
OBJECTIVE
To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells.


METHODS
3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyl-tetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits.


RESULTS
The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly.


CONCLUSIONS
Hydroxycamptothecin can induce human hepatoma Hep G2 cells to differentiation.
Zhang
X
X
Department of Pharmacology, Shanghai Institute of Material Medical, Chinese Academy of Sciences, Shanghai 200031, China.
Zhou
Y
Y
Xu
B
B
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects*; D006801:Humans
D000972:Antineoplastic Agents, Phytogenic; C028098:10-hydroxycamptothecin; D002166:Camptothecin