[Experimental study of influence of yiqi huoxue serial recipes on basement membrane in membranous nephritis in rabbits].
OBJECTIVE
To clucidate the mechanism of the therapeutic effect of Yiqi Huoxue (YQHX) serial recipes on membranous nephritis.


METHODS
Forty-nine New Zealand male rabbits were made to menbranous nephritis model by cation bovine serum albumin and divided into 5 groups, the group A (treated by Qingre Moshen granule), B (treated by Bushen Moshen granule), C (treated by steroid), D (the control group) and E (the normal group). Twenty-four hours' urinary protein content of the animals was determined every week, and plasma albumin, blood lipid, renal function and prostaglandins were tested by the end of experiment. And pathological changes of basement membrane were observed by using light, electronic and immunofluorescent microscopy with polyethylene imine stain.


RESULTS
The 24 hours urinary protein content, plasma albumin and blood lipid in the group A and B were lower than those in the control group significantly, P < 0.01 or 0.05, while those in the group C and the D were similar, P > 0.05. In comparing the group A and B with the group C, the difference was also significant, P < 0.05. Light, electronic and immunofluorescent microscopic examination all showed that the pathologic changes in the group A, B and C were lesser than that of the control, the effect was in the order A > B > C.


CONCLUSIONS
YQHX serial recipes can reduce urinary protein content, elevate plasma albumin level, restore the charge barrier effect of and attenuate the immune complex deposition on the basement membrane of glomeruli.
Hu
Z
Z
Department of Nephrology, Longhua Hospital, Shanghai University of TCM, Shanghai (200032).
Tang
L
L
Chen
Y
Y
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015433:Glomerulonephritis, Membranous / Q000097:blood* / Q000473:pathology / Q000652:urine; D008297:Male; D011507:Proteinuria / Q000378:metabolism; D011817:Rabbits; D011897:Random Allocation; D012709:Serum Albumin / Q000378:metabolism*; D013929:Thromboxane B2 / Q000097:blood
D004365:Drugs, Chinese Herbal; D012709:Serum Albumin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha