title: Human hematopoiesis in murine embryos after injecting human cord blood-derived hematopoietic stem cells into murine blastocysts.
abstract: At different developmental stages, candidate human hematopoietic stem cells (HSCs) are present within the CD34+ CD38- population. By means of xenotransplantation, such CD34+CD38- cells were recently shown to engraft the hematopoietic system of fetal sheep and nonobese diabetic severe combined immunodeficient adult mice. Here it is demonstrated that, after their injection into murine blastocysts, human cord blood (CB)-derived CD34+ and CD34+ CD38- cells repopulate the hematopoietic tissues of nonimmunocompromised murine embryos and that human donor contribution can persist to adulthood. It is further observed that human hematopoietic progenitor cells are present in murine hematopoietic tissues of midgestational chimeric embryos and that progeny of the injected human HSCs activate erythroid-specific gene expression. Thus, the early murine embryo provides a suitable environment for the survival and differentiation of human CB CD34+ CD38- cells.
authors.affiliation: Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Harder
authors.forename: Reinhard
authors.initials: R
authors.lastname: Henschler
authors.forename: Ilse
authors.initials: I
authors.lastname: Junghahn
authors.forename: Marinus C
authors.initials: MC
authors.lastname: Lamers
authors.forename: Albrecht M
authors.initials: AM
authors.lastname: Müller
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D019070:Cell Lineage; D002470:Cell Survival; D002678:Chimera / Q000235:genetics*; D004624:Embryo Transfer; D005312:Fetal Blood / Q000166:cytology*; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D006410:Hematopoiesis; D006413:Hematopoietic System / Q000196:embryology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D014183:Transplantation, Heterologous
chemical_list: D018952:Antigens, CD34; D005914:Globins