Adaptation of wild-type measles virus to tissue culture.
Measles has a host range restricted to humans and monkeys in captivity. Fresh measles virus (MV) isolates replicate readily in several human and simian B-cell lines but need a period of adaptation to other types of cells. The identification of CD46 and CD150 (SLAM) as cellular receptors for MV has helped to clarify certain aspects of the immunobiology of MV infections. We have examined the properties of an MV wild-type strain grown in the epithelial cell line Vero. After adaptation, this virus expressed high levels of both the viral glycoproteins (hemagglutinin and fusion protein) but did not induce fusion (syncytia). No changes in the amino acid sequence were found in either of the viral glycoproteins. Using several approaches, the Vero-adapted virus could not be shown to interact with CD46 either in the initiation or during the course of infection. The presence of human SLAM expressed in the Vero cells rapidly gave rise to fusion and lower yields of infectious virus.
Kouomou
Diane Waku
DW
INSERM U 404, CERVI, 69365 Lyon Cedex 07, France.
Wild
T Fabian
TF
D000222:Adaptation, Physiological; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D006023:Glycoproteins / Q000378:metabolism; D006389:Hemagglutinins, Viral / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D008459:Measles virus / Q000378:metabolism / Q000502:physiology*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000378:metabolism; D011956:Receptors, Cell Surface; D011991:Receptors, Virus / Q000378:metabolism; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D015703:Antigens, CD; C497857:CD46 protein, human; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D007136:Immunoglobulins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C000604059:SLAMF1 protein, human; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1