Organization of six functional mouse alcohol dehydrogenase genes on two overlapping bacterial artificial chromosomes.
Mammalian alcohol dehydrogenases (ADH) form a complex enzyme system based on amino-acid sequence, functional properties, and gene expression pattern. At least four mouse Adh genes are known to encode different enzyme classes that share less than 60% amino-acid sequence identity. Two ADH-containing and overlapping C57BL/6 bacterial artificial chromosome clones, RP23-393J8 and -463H24, were identified in a library screen, physically mapped, and sequenced. The gene order in the complex and two new mouse genes, Adh5a and Adh5b, and a pseudogene, Adh5ps, were obtained from the physical map and sequence. The mouse genes are all in the same transcriptional orientation in the order Adh4-Adh1-Adh5a-Adh5b-Adh5ps-Adh2-Adh3. A phylogenetic tree analysis shows that adjacent genes are most closely related suggesting a series of duplication events resulted in the gene complex. Although mouse and human ADH gene clusters contain at least one gene for ADH classes I-V, the human cluster contains 3 class I genes while the mouse cluster has two class V genes plus a class V pseudogene.
Szalai
Gabor
G
Department of Biological Sciences, University of South Carolina, Columbia 29208, USA.
Duester
Gregg
G
Friedman
Robert
R
Jia
Honggui
H
Lin
ShaoPing
S
Roe
Bruce A
BA
Felder
Michael R
MR
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D006801:Humans; D051379:Mice; D010802:Phylogeny; D014158:Transcription, Genetic
D000426:Alcohol Dehydrogenase