[The effect of cyclooxygenase-2 inhibitor on acute lung injury].
OBJECTIVE
To investigate changes of cyclooxygenase-2 (COX-2) mRNA expression and intervention effect of selective cyclooxygenase-2 inhibitor Meloxicam on acute lung injury (ALI).


METHODS
We measured COX-2 mRNA expression by reverse-transcription polymerase chain reaction in rat lung with ALI induced by lipopolysaccharide, and observed changes of prostaglandins (PGs), PaO2 and histopathology.


RESULTS
Resting rat lung expressed a little COX-2 mRNA; expression of cyclooxygenase-2 mRNA was up-regulated significantly in the lung after rats were injured by LPS. Selective COX-2 inhibitor Meloxicam alleviated histopathologic damage in the lung, inhibited production of PGs and attenuated PaO2 decline.


CONCLUSIONS
COX-2 is a main isoform responsible for enhancing PGs production during ALI. Meloxicam can inhibit activity of COX-2 and may be useful in the treatment of ALI.
Wang
J
J
Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Changqing 400037.
Mao
B
B
Chen
Z
Z
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001784:Blood Gas Analysis; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D000077239:Meloxicam; D010100:Oxygen / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D051381:Rats; D012128:Respiratory Distress Syndrome / Q000139:chemically induced* / Q000473:pathology; D013843:Thiazines / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D008070:Lipopolysaccharides; D013843:Thiazines; D013844:Thiazoles; D015121:6-Ketoprostaglandin F1 alpha; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D010100:Oxygen; D000077239:Meloxicam