Molecular cytogenetic analysis of a novel high-grade canine T-lymphoblastic lymphoma demonstrating co-expression of CD3 and CD79a cell markers.
We present the molecular cytogenetic analysis of a novel case of canine lymphoma, in a nine-year-old entire male collie cross retriever dog that presented with an enlarged prescapular lymph node. A diagnosis of high-grade lymphoblastic lymphoma was made by histological evaluation of fixed lymph node biopsy sections, whilst immunohistochemical analyses demonstrated co-expression of B- and T-cell antigens (CD79a and CD3) by 95% of lymphomatous cells. Comparative genomic hybridisation (CGH) analysis detected loss of dog chromosomes 11, 30 and 38 and gain of chromosome 36 within the lymphoma biopsy specimen. These findings correlated with direct cytogenetic analysis of tumour metaphases using whole chromosome paint probes representing each of these four chromosomes. This study represents the first report of the combined application of both direct and indirect cytogenetic techniques for the analysis of recurrent chromosome aberrations in canine cancer.
Thomas
R
R
Genetics Section, Animal Health Trust, Newmarket, Suffolk, UK. rthomas@hgmp.mrc.ac.uk
Smith
K C
KC
Gould
R
R
Gower
S M
SM
Binns
M M
MM
Breen
M
M
D000818:Animals; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy / Q000662:veterinary; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism*; D051925:CD79 Antigens; D002869:Chromosome Aberrations / Q000662:veterinary; D020223:Chromosome Painting / Q000662:veterinary; D020732:Cytogenetic Analysis / Q000662:veterinary*; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D007150:Immunohistochemistry; D007621:Karyotyping / Q000662:veterinary; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology / Q000378:metabolism*
D015703:Antigens, CD; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D051925:CD79 Antigens; D011947:Receptors, Antigen, B-Cell