[Reverse in situ hybridization of DNA probes of anomalous chromosomes in diagnosing chromosome pathologies].
The results of analysis of congenital chromosomal pathologies and chromosomal rearrangements upon the occurrence of haematological diseases, which was involved constructing DNA libraries of abnormal chromosomes and subsequent reverse CISS hybridization have been considered. High effectiveness of this approach for analysis of chromosomal translocations, deletions of chromosomal regions, minor extra chromosomes, and large marker chromosomes with complex organization was shown. The possibility of implementation of this approach and its large-scale application in medical and genetic studies of congenital developmental pathologies and chromosomal diagnostics of haematological diseases has been discussed.
Rubtsov
N B
NB
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia. rubt@bionet.nsc.ru
Karamysheva
T V
TV
Matveeva
V G
VG
Sablina
O V
OV
Sosnitskaia
S V
SV
Andreenkova
O V
OV
Gaĭner
T A
TA
Degtiareva
M M
MM
D002869:Chromosome Aberrations; D015342:DNA Probes; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D012426:Russia
D015342:DNA Probes
Obratnaia in situ gibridizatsiia DNK-zondov anomal'nykh khromosom v diagnostike khromosomnykh patologiĭ.