Neutrophil adhesion molecules in colorectal surgery: effect of filgrastim given perioperatively.
OBJECTIVE
To assess whether the postoperative expression of neutrophil adhesion molecules CD11b/CD18 (Mac-1) and CD62L (L-selectin) would differ in peripheral blood, peritoneal fluid and wound fluid in patients operated on for colorectal conditions, and to analyse the effect of perioperative filgrastim on their expression.


METHODS
Prospective randomised double-blind placebo-controlled clinical study.


METHODS
University hospital, Finland.


METHODS
Thirty consecutive patients undergoing elective colorectal operations (15 in each group).


METHODS
The patients were prospectively randomised to receive either filgrastim or placebo. Expression of neutrophil adhesion molecules was measured 48 hours postoperatively in peripheral blood, peritoneal fluid, and wound fluid by flow cytometry.


RESULTS
Postoperative neutrophil CD11b/CD18 expression was higher in both wound fluid and peritoneal fluid than in peripheral blood in the placebo group. Simultaneously, the expression of neutrophil CD62L was higher in peripheral blood than in peritoneal fluid or wound fluid in both groups. Filgrastim caused increased postoperative expression of neutrophil CD11b/CD18 in peripheral blood but not in peritoneal fluid or wound fluid.


CONCLUSIONS
Postoperative expression of neutrophil adhesion molecules differs at the local operation site from that in peripheral blood. Filgrastim increases only blood neutrophil CD11b/CD18 expression.
Wiik
H
H
Department of Surgery, University of Oulu, Finland. Heikki.Wiik@ppshp.fi
Syrjälä
H
H
Karttunen
R
R
Bloigu
A
A
Haukipuro
K
K
D015815:Cell Adhesion Molecules / Q000032:analysis*; D003107:Colorectal Surgery; D004311:Double-Blind Method; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D019041:L-Selectin / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000032:analysis; D018375:Neutrophil Activation; D011446:Prospective Studies; D011994:Recombinant Proteins; D018709:Statistics, Nonparametric
D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim