[Interaction of E. coli cells with ascorbic acid and sodium nitrite studied by ESR method].
Ascorbic acid, an effective modulator and regulator of cell metabolism, was shown to induce the production of nitric oxide in E. coli cells. This process was detected by EPR spectroscopy as the generation of a spectral signal typical of nitrosyl-iron-sulfur centers (Fe-S-NO) under anaerobic conditions. Incubation of E. coli cells in the presence of ascorbic acid under aerobic conditions was shown to be accompanied by sodium nitrite formation. It is suggested that ascorbic acid is capable of supporting the system of energy supply to cells in hypoxia caused by reduced oxygen content or treatment with sodium nitrite.
Zhumbaeva
T T
TT
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 117977 Russia.
Baĭder
L M
LM
Volodina
L A
LA
Kuropteva
Z V
ZV
D001205:Ascorbic Acid / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012977:Sodium Nitrite / Q000494:pharmacology*
D009569:Nitric Oxide; D012977:Sodium Nitrite; D001205:Ascorbic Acid; D010100:Oxygen
Issledovanie metodom EPR vzaimodeĭstviia kletok E. coli s askorbinovoĭ kislotoĭ i nitritom natriia.