title: Genotoxicity of some sulfur dyes on tadpoles (Rana hexadactyla) measured using the comet assay.
abstract: This report presents the results of a genotoxicity study to evaluate the DNA damage caused by four sulfur dyes used in the textile and tannery industries. Alkaline single-cell gel electrophoresis assay (SCGE) was performed on erythrocytes from Rana hexadactyla tadpoles following whole-body exposure to increasing concentrations of the dyes. The dyes, along with their active ingredients, were Sandopel Basic Black BHLN, Negrosine, Dermapel Black FNI, and Turquoise Blue. The dye-treated tadpoles showed significant DNA damage, measured as mean DNA length:width ratio, when compared with unexposed control animals. Among the four tested dyes Sandopel Basic Black BHLN appears to be highly genotoxic, Dermapel Black FNI was least genotoxic, and Negrosine and Turquoise Blue were moderately toxic to R. hexadactyla tadpoles. The tadpoles showed a significant reduction in DNA damage when placed in dechlorinated tap water after exposure for a 24-hr period to the dye solutions.
authors.affiliation: Department of Environmental Science, PSG College of Arts and Science, Coimbatore 641014, India. p_rajaguru@lycos.com
authors.forename: P
authors.initials: P
authors.lastname: Rajaguru
authors.forename: R
authors.initials: R
authors.lastname: Kalpana
authors.forename: A
authors.initials: A
authors.lastname: Hema
authors.forename: S
authors.initials: S
authors.lastname: Suba
authors.forename: B
authors.initials: B
authors.lastname: Baskarasethupathi
authors.forename: P A
authors.initials: PA
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Kalaiselvi
mesh_terms: D000814:Aniline Compounds / Q000633:toxicity; D000818:Animals; D004396:Coloring Agents / Q000633:toxicity*; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects; D007814:Larva; D009930:Organic Chemicals; D011898:Ranidae; D013457:Sulfur Compounds
chemical_list: D000814:Aniline Compounds; D004396:Coloring Agents; D009930:Organic Chemicals; D013457:Sulfur Compounds; C449190:acid black 191; C449189:sandopel basic black; C449191:reactive blue 15; C002712:nigrosin