title: DNA microarray analyses of genes elicited by ultrasound in human U937 cells.
abstract: The gene expression of human histiocytic lymphoma cell line U937 at 6 h after 1 MHz ultrasound treatment in the presence of Ar or N(2)O gas was examined by DNA microarrays. Of the 9,182 genes analyzed, only the keratin gene was identified as down-regulated in the cells exposed to ultrasound in the presence of N(2)O where no internal cavitation was observed. In contrast, five up-regulated and two down-regulated genes were identified in the cells exposed to ultrasound in the presence of Ar where internal cavitation was apparently observed. Six changes of the gene expression were confirmed by the semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Gene expression of heme oxygenase was augmented by a factor of 6.6 in microarray and by 4.0 by RT-PCR. These results indicate that internal cavitation increased the expression of genes responsive to oxidative stress in sonicated cells but non-inertial cavitation had minimal effects on gene expression.
authors.affiliation: Molecular Genetics Research Center, Department of Radiological Sciences, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama City, Toyama 930-0194, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
authors.forename: Ryohei
authors.initials: R
authors.lastname: Ogawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Mori
mesh_terms: D017209:Apoptosis; D001128:Argon / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000378:metabolism; D005609:Free Radicals; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis; D006801:Humans; D009609:Nitrous Oxide / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D020298:U937 Cells; D014465:Ultrasonics; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D005609:Free Radicals; D012333:RNA, Messenger; D001128:Argon; D006419:Heme Oxygenase (Decyclizing); D009609:Nitrous Oxide