title: Vesicular transport of soluble substances into mouse milk.
abstract: Utilizing a novel protocol to study transport of substances into mouse milk in situ, we have shown that many "fluid-phase" markers are taken up by mammary epithelial cells and deposited in milk. Since the tight junctions are closed and impermeable even to small molecules, extra-alveolar substances (those not synthesized by the alveolar cells) must be transported into the milk by the epithelial cells themselves. The markers we have used include dextran, lucifer yellow dye, horseradish peroxidase, and albumin. Using these markers and immunostaining for endogenous proteins, we have visualized transcytotic vesicles involved in transporting these markers to milk.
authors.affiliation: Department of Physiology, University of Colorado Health Sciences Center, Denver 80262, USA.
authors.forename: J
authors.initials: J
authors.lastname: Monks
authors.forename: M C
authors.initials: MC
authors.lastname: Neville
mesh_terms: D000818:Animals; D001692:Biological Transport; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005456:Fluorescent Dyes; D007070:Immunoglobulin A / Q000032:analysis / Q000378:metabolism; D007457:Iodine Radioisotopes; D007774:Lactation; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008892:Milk / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D007070:Immunoglobulin A; D007457:Iodine Radioisotopes; D008894:Milk Proteins