[Experimental study on the effect of restorative dentin formation with human dentin phosphoprotein in immature permanent teeth of minipig].
OBJECTIVE
To investigate the function of human dentin phosphoprotein(DPP) on dentine mineralization in vivo.


METHODS
Pulp capping with human DPP was made in the permanent teeth of minipigs with calcium hydroxide and zinc oxide eugenol cement as controls. The minipigs were sacrificed at intervals of 14, 30, 90 days respectively after operation.


RESULTS
The histopathological results revealed that pulp cells had developed into odontoblastoid cells around the major restorative dentin body after two weeks. There was complete restorative dentin bridge a month later. The bridge became thick and dense, and mainly was tubular dentin in 3 month. There were well developed odontoblasts under the bridge. In the calcium hydroxide capping group, inflammatory cytonecrosis zone was found in the surface of dental pulp and only a little calcial bodies formed in two weeks. Complete restorative dentin bridge was formed in 1 to 3 months, the speed of dentin bridge formation was not as quick as that of DPP group.


CONCLUSIONS
DPP can induce pulp cells to develop into odontoblasts and formation of dentin bridge with little stimulation to the dental pulp. This study suggests that DPP plays an important role in the biomineralization of dentin development and restoration.
Ouyang
Y
Y
School of Stomatology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510060.
Li
Y
Y
Liu
X
X
D000818:Animals; D003782:Dental Pulp / Q000187:drug effects; D003785:Dental Pulp Capping; D003793:Dental Restoration, Permanent / Q000379:methods*; D003809:Dentin, Secondary / Q000187:drug effects*; D006801:Humans; D010750:Phosphoproteins / Q000494:pharmacology*; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
D010750:Phosphoproteins