Differential inhibition of human CYP1A1 and CYP1A2 by quinidine and quinine.
1. The inhibition of recombinant CYP1A1 and CYP1A2 activity by quinidine and quinine was evluated using ethoxyresorutin O-deethylation, phenacetin O-deethylation and propranolol desisopropylation as probe catalytic pathways. 2. With substrate concentrations near the Km of catalysis, both quinidine and quinine potently inhibited CYP1A1 activity with [I](0.5) approximately 1-3 microM, whereas in contrast, there was little inhibition of CYP1A2 activity. The Lineweaver-Burk plots with varying inhibitor concentrations suggested that inhibition by quinidine and quinine was competitive. 3. There was only trace metabolism of quinidine by recombinant CYP1A1, whereas rat liver microsomes as a control showed extensive consumption of quinidine and metabolite production. 4. This work suggests that quinidine is a non-classical inhibitor of CYP1A1 and that it is not as highly specific at inhibiting CYP2D6 as previously thought.
Ching
M S
MS
Department of Medicine, University of Melbourne, Austin & Repatriation Medical Centre, Victoria, Australia. ching@austin.unimelb.edu.au
Blake
C L
CL
Malek
N A
NA
Angus
P W
PW
Ghabrial
H
H
D000082:Acetaminophen / Q000378:metabolism; D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D019388:Cytochrome P-450 CYP1A2 / Q000235:genetics / Q000378:metabolism; D065609:Cytochrome P-450 CYP1A2 Inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D010615:Phenacetin / Q000378:metabolism / Q000494:pharmacology; D011802:Quinidine / Q000494:pharmacology*; D011803:Quinine / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D015003:Yeasts / Q000235:genetics
D065609:Cytochrome P-450 CYP1A2 Inhibitors; D004791:Enzyme Inhibitors; D010078:Oxazines; D011994:Recombinant Proteins; D000082:Acetaminophen; C007649:ethoxyresorufin; D011803:Quinine; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D010615:Phenacetin; D011802:Quinidine