[Bispecific McAb mediated multiple tumor cytotoxicity in gastric cancer].
OBJECTIVE
McAb against CD3 and McAb against TNP were combined into bispecific McAb by chemical methods. With this bispecific McAb, human peripheral blood lymphocytes (PBLS) were introduced to in vitro and in vivo cytotoxic test of any tumor cells by different TNP-McAb against gastric cancer.


METHODS
The activity of bispecific McAb prepared by chemical method, was detected by enzyme-linked immunosorbent assay (ELISA). The bispecific McAb was identified by bridge ELISA. Ouchterlony test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Cytotoxic test of tumor cells in vitro and tumor growth inhibition test in vivo were performed for the bispecific McAb function.


RESULTS
Anti-CD3 and Anti-TNP McAbs were successfully combined into bispecific McAb which could conjugate any TNP-McAb against gastric cancer and induce PBLS to cytotoxic test of gastric cancer cells in vitro and in vivo.


CONCLUSIONS
The bispecific McAb may very will be useful in the immuno-biotherapy of tumors.
Wan
W
W
Beijing Medical University School of Oncology, Beijing Institute for Cancer Research, Beijing Cancer Hospital, Beijing 100036, China.
Gao
X
X
Zhang
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D000911:Antibodies, Monoclonal