title: Integrative cartilage repair: adhesive strength is correlated with collagen deposition.
abstract: Procedures to repair focal articular cartilage defects often result in poor integration between the host cartilage and the graft tissue, and this may be related to the lack of matrix deposition and the death of chondrocytes near a cut cartilage surface. The objective of this study was to determine if cartilage repair was related to deposition of newly synthesized collagen. The mechanical integration that occurred between two live adult bovine cartilage blocks cultured in partial apposition for two weeks was correlated with [3H]proline incorporation, a measure of protein synthesis, of which more than 66% was accounted for by collagen. A similar level of mechanical integration occurred in sample pairs consisting of a live and killed cartilage block, and this adhesive strength was also correlated with [3H]proline deposition into both the live and the killed blocks. In these samples, the [3H]proline deposited into the killed cartilage appeared to originate from chondrocytes in the live cartilage, since live cells were not detected in the killed cartilage block by either viability staining or [35S]sulfate incorporation. These results suggest a mechanism of integrative cartilage repair in which live chondrocytes within cartilage secrete matrix molecules that are components of a collagen network, and subsequent deposition of these molecules near the repair interface contributes to functional integration.
authors.affiliation: Department of Bioengineering and Institute for Biomedical Engineering, University of California, San Diego, La Jolla 92093-0412, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: DiMicco
authors.forename: R L
authors.initials: RL
authors.lastname: Sah
mesh_terms: D000268:Adhesiveness; D000818:Animals; D002356:Cartilage / Q000502:physiology* / Q000601:surgery*; D002417:Cattle; D003094:Collagen / Q000502:physiology*; D011392:Proline / Q000378:metabolism
chemical_list: D003094:Collagen; D011392:Proline