title: [The role of chitinase in antifungal activity of Bacillus sp. 739].
abstract: Investigation of the crude extracellular chitinase of Bacillus sp. 739, an antagonist of phytopathogenic fungi, discerned a relationship between the chitinase and antifungal activities of this bacterium. Purified chitinase lost its ability to inhibit the growth of micromycetes. The antagonistic (antifungal) activity of crude chitinase was found to be located in a low-molecular-weight fraction of the enzyme, which does not possess chitinase activity. Both crude and purified chitinase were able to lyse the cell walls of intact mycelium. Accordingly, it may be inferred that the antagonistic activity of Bacillus sp. 739 against micromycetes is largely determined by low-molecular-weight nonenzymatic substances whereas the role of chitinase is to utilize chitin, which is ubiquitously present in soil.
authors.affiliation: Institute of Biology, Ufa Research Center, Russian Academy of Sciences, pr. Oktyabrya 69, Ufa, 450054 Russia. mlnt@anrb.ru
authors.forename: A I
authors.initials: AI
authors.lastname: Melen'tiev
authors.forename: G E
authors.initials: GE
authors.lastname: Aktuganov
authors.forename: N F
authors.initials: NF
authors.lastname: Galimzianova
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000201:enzymology; D002473:Cell Wall; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002850:Chromatography, Gel; D003470:Culture Media; D003904:Mitosporic Fungi / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003470:Culture Media; D002688:Chitinases
vernacular_title: Rol' khitinazy v proiavlenii antigribnoĭ aktivnosti shtammom Bacillus sp. 739.