[Genetic polymorphism of polyacrylamide gel electrophoresis loci in patients after non-myeloablative allogeneic peripheral blood stem cell transplantation].
OBJECTIVE
To understand the information of donor and recipient in the mixed hematopoietic chimerism after non-myeloablative allogeneic peripheral blood stem cell transplantation(NM-APBSCT).


METHODS
DNA samples were extracted with phenol/chloroform method and were amplified by PCR technique in heparin-blood or heparin-bone-marrow. The PCR products were analyzed using polyacrylamide gel electrophoresis and silver staining means.


RESULTS
The amplified fragment length polymorphism was found in the short tandem repeat loci of 10 healthy persons and 8 leukemia patients who were not treated with hematopoietic stem cells transplantation; the bands of silver staining originated from donors and recipients were found in 3 patients after NM-APBSCT, but the brightness in the bands of donor and recipient was different.


CONCLUSIONS
Polymorphism for mixed hematopoietic chimerism can be estimated timely, sensitively and exactly; and the results may be used to guide adoptive immunotherapy for patients after NM-APBSCT. The silver means were simple without contamination of isotopes and without using special equipment. The methods may benefit common hospitals to develop work in this respect.
Li
X
X
Department of Hematology, PLA General Hospital, Beijing 100853, China.
Yu
L
L
Lou
F
F
D004273:DNA, Neoplasm / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007938:Leukemia / Q000097:blood / Q000235:genetics / Q000628:therapy; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020080:Tandem Repeat Sequences / Q000235:genetics; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
D004273:DNA, Neoplasm