[Effect of IGF1 receptor gene antisense oligodeoxynucleotide on T24 urinary bladder cancer cells].
OBJECTIVE
To evaluate the effects of autocrine blockage on T24 urinary bladder cancer cells.


METHODS
Semiquantitative RT-PCR, MTT determination, 3H thymidine incorporation, flow cytometry and electron microscopy were used to study the effects of antisense oligodexynucleotide (ODN) targeted against insulin-like growth factor 1 receptor (IGF1R) gene on IGF1R gene expression, drug sensitivity, proliferation and apoptosis of T24 cells.


RESULTS
After being treated with antisense ODN specific for IGF1R gene for 48 hours, the intrinsic IGF1R mRNA expression of T24 cells reduced approximately by 72.9%, which caused decrease in survival and increased sensitivity to mitomycin-induced apoptosis(P < 0.05).


CONCLUSIONS
To block the autocrine loop with IGF1R antisense oligonucleotide may serve as a potential therapeutic approach to bladder cancer.
Sun
H
H
Department of Urology, Shanghai First People's Hospital, Shanghai 200080, China.
Fan
J
J
Tang
X
X
D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D008854:Microscopy, Electron; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D017526:Receptor, IGF Type 1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000648:ultrastructure
D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D017526:Receptor, IGF Type 1