title: Actinobacillus actinomycetemcomitans-induced expression of IL-1alpha and IL-1beta in human gingival epithelial cells: role in IL-8 expression.
abstract: Gingival epithelial cells (GEC) are the first cells of the host that encounter the periodontal pathogens. and therefore their role in the initiation of the inflammatory response is critical. We aimed to: 1) characterize the expression of interleukin (IL)- Ialpha and IL-Ibeta in human gingiva and cultured GEC: 2) demonstrate the ability of A. actinomycetemcomitans extracts to upregulate IL-1alpha, IL-1beta and IL-8 expression in GEC in vitro: and 3) characterize the role of IL-1alpha and IL-1beta in the induction of IL-8 expression in GEC in vitro. Ten gingival biopsies (5 inflamed and 5 controls) and cultured GEC were examined for IL-1alpha and IL-Ibeta using immunohistochemical techniques. GEC were also challenged with A. actinomycetemcomitans extracts or IL-1alpha, and secretion of IL-1 and IL-8 was determined by ELISA. In vivo, IL-lalpha and IL-1beta were localized in the gingival epithelium and the infiltrating leukocytes. In vitro, A. actinomycetemcomitans extracts induced a time-dependent expression of IL-1alpha, IL-1beta and IL-8 in GEC. IL-1 inhibitors did not affect A. actinomycetemcomitans-induced IL-8. although they inhibited IL-8 induced by IL-1alpha or IL-1beta. In conclusion, GEC are a major source of IL-1alpha and IL-1beta in the periodontium, which in turn induce additional inflammatory mediators such as IL-8. Therefore GEC can be a potential target for therapeutic intervention in the future.
authors.affiliation: Department of Periodontology, University of Connecticut, School of Medicine, School of Dental Medicine, Farmington 06030-3105, USA.
authors.forename: A
authors.initials: A
authors.lastname: Sfakianakis
authors.forename: C E
authors.initials: CE
authors.lastname: Barr
authors.forename: D L
authors.initials: DL
authors.lastname: Kreutzer
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology*; D002461:Cell Line, Transformed; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology; D005881:Gingiva / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D010518:Periodontitis / Q000378:metabolism / Q000382:microbiology; D015854:Up-Regulation
chemical_list: D007375:Interleukin-1; D016209:Interleukin-8