[Study on the anti-müllerian hormone served as a marker for granulosa cell tumor of ovary].
OBJECTIVE
To probe the experimental basis for anti-mullerian hormone(AMH) served as a specific tumor label in diagnosis of granulosa cell tumor(GCT) of ovary.


METHODS
The expression of AMH and its receptor mRNA in early and late GCT of ovary in transgenic mice was studied by using immunohistochemistry, in stiu hybridization and Northern hybridization. Furthermore, the expression of AMH and its receptor mRNA in cultured GCT cells was also determined by using radioiodine labeling method and Northern hybridization.


RESULTS
In the early ovary GCT of transgenic mice, immunohistochemistry showed that AMH was positively stained as brownish yellow granulars, which were located in the cell membrane of the tumor, in situ hybridization showed its receptor mRNA was positive as blue granulars, which were also located in the cell membrane. The positive rate of both AMH and its receptor mRNA was 100%. Results by northern hybridization also showed that the positive rate of AMH and its receptor mRNA in the early ovary GCT and GCT cells was 100%. Radioautography indicated that there was a radioactive bright point, the binding rate of 125I labeled AMH with tumor cells reached 100%.


CONCLUSIONS
AMH can be served as a specific tumor marker in the diagnosis of difficult and complicated tumors of ovary, and in the diagnosis, treatment and follow-up survey of the early GCT.
Long
W
W
Department of Obstetrics and Gynecology, RuiJin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Wang
W
W
Rey
R
R
D000818:Animals; D054304:Anti-Mullerian Hormone; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006023:Glycoproteins; D006106:Granulosa Cell Tumor / Q000175:diagnosis* / Q000628:therapy; D006131:Growth Inhibitors / Q000032:analysis* / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000628:therapy; D018000:Receptors, Peptide / Q000032:analysis / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta; D013735:Testicular Hormones / Q000032:analysis* / Q000235:genetics
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D006131:Growth Inhibitors; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; D013735:Testicular Hormones; C084621:anti-Mullerian hormone receptor; D054304:Anti-Mullerian Hormone