Vancomycin monitoring in term newborns: comparison of peak and trough serum concentrations determined by high performance liquid chromatography and fluorescence polarization immunoassay.
BACKGROUND
Peak and trough serum concentrations of vancomycin were determined in term newborn infants with confirmed or suspected Staphylococcus sp sepsis by high performance liquid chromatography and flourescence polarization immunoassay.


OBJECTIVE
To statistically compare the results of the high performance liquid chromatography and flourescence polarization immunoassay techniques for measuring serum vancomycin concentrations.


METHODS
Eighteen peak and 20 trough serum samples were assayed for vancomycin concentrations using high performance liquid chromatography and flourescence polarization immunoassay from October 1995 to October 1997.


RESULTS
The linear correlation coefficients for high performance liquid chromatography and flourescence polarization immunoassay were 0.27 (peak, P = 0.110) and 0.26 (trough, P = 0.1045) respectively, which were not statistically significant.


CONCLUSIONS
There was wide variation in serum vancomycin concentrations determined by high performance liquid chromatography as compared with those determined by flourescence polarization immunoassay. There was no recognizable pattern in the variability; in an apparently random fashion, the high performance liquid chromatography measurement was sometimes substantially higher than the flourescence polarization immunoassay measurement, and at other times it was substantially lower.
Feferbaum
R
R
Department of Pediatrics, Hospital das Clínicas, Faculty of Medicine, University of São Paulo, Brazil.
Kobol Machado
J K
JK
de Albuquerque Diniz
E M
EM
Okay
T S
TS
Santos
S R
SR
Ceccon
M E
ME
Krebs
V L
VL
de Araújo
M C
MC
Costa Vaz
A
A
D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007231:Infant, Newborn; D008991:Monitoring, Physiologic; D018805:Sepsis / Q000097:blood* / Q000188:drug therapy; D013203:Staphylococcal Infections / Q000097:blood* / Q000188:drug therapy; D014640:Vancomycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D014640:Vancomycin