Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors.
Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.
Lois
Carlos
C
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Hong
Elizabeth J
EJ
Pease
Shirley
S
Brown
Eric J
EJ
Baltimore
David
D
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D015153:Blotting, Western; D019070:Cell Lineage; D003433:Crosses, Genetic; D004274:DNA, Recombinant; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000378:metabolism* / Q000821:virology; D005260:Female; D018628:Gene Dosage; D015870:Gene Expression; D020868:Gene Silencing; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000235:genetics; D017919:Hepatitis B Virus, Woodchuck / Q000235:genetics; D016086:Lentivirus / Q000235:genetics*; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011533:Proviruses / Q000235:genetics; D051381:Rats; D019076:Transgenes; D016662:Virus Integration
D004274:DNA, Recombinant; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins