title: Evaluation of the genotoxicity of zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based root canal sealers by comet assay.
abstract: Three main types of root canal sealer are currently commonly used in pulp treatment: zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based sealers. In the present study, the genotoxicity of sealer on oral carcinoma cells was evaluated by single-cell gel electrophoresis assay (comet assay). The whole length of the comet and the diameter of the head were measured using an image analysis system. The results were analyzed by one-way analysis of variance to compare the various means. The zinc oxide eugenol-based sealers (Canals, Canals-N, and Tubilseal) did not always cause a dose-dependent increase in genotoxicity. The resin-based sealers (Topseal, AH 26, and AH Plus) caused a dose-dependent increase in genotoxicity, but no such effect was seen with the calcium hydroxide-based sealer (Sealapex). The highest level of DNA damage was induced by the resin-based sealers.
authors.affiliation: Department of Endodontics, Chung Shan Medical and Dental College, Taichung, Taiwan.
authors.forename: T H
authors.initials: TH
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: C T
authors.initials: CT
authors.lastname: Kao
mesh_terms: D000704:Analysis of Variance; D001729:Bismuth / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell; D020552:Comet Assay; D004249:DNA Damage; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity*; D006801:Humans; D008709:Methenamine / Q000633:toxicity; D009062:Mouth Neoplasms; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012459:Salicylates / Q000633:toxicity; D012834:Silver / Q000633:toxicity; D014025:Titanium / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity*
chemical_list: D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; C115110:Topseal; D015035:Zinc Oxide-Eugenol Cement; C112894:canals sealer; C534916:epoxy resin-based root canal sealer; C055601:Sealapex; D012834:Silver; C028044:epoxy resin AH-26; C048769:tubliseal; D014025:Titanium; D008709:Methenamine; D002126:Calcium Hydroxide; D001729:Bismuth