title: The catalytic mechanism of nucleoside diphosphate kinases.
abstract: Nucleoside diphosphate kinases catalyze the reversible transfer of the gamma phosphate of nucleoside triphosphates to nucleoside diphosphates. This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. We also briefly discuss the physiological relevance of in vitro studies.
authors.affiliation: Institut de Biochimie et Génétique Cellulaires, UMR 5095 University of Bordeaux-2 and CNRS, France. ioan.lascu@ibgc.u-bordeaux2.fr
authors.forename: I
authors.initials: I
authors.lastname: Lascu
authors.forename: P
authors.initials: P
authors.lastname: Gonin
mesh_terms: D000818:Animals; D002384:Catalysis; D002413:Cations, Divalent; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008670:Metals; D009701:Nucleoside-Diphosphate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D013379:Substrate Specificity
chemical_list: D002413:Cations, Divalent; D004791:Enzyme Inhibitors; D008670:Metals; D009701:Nucleoside-Diphosphate Kinase