title: Validation of a multicolor interphase fluorescence in situ hybridization assay for detection of transitional cell carcinoma on fresh and archival thin-layer, liquid-based cytology slides.
abstract: OBJECTIVE
  To evaluate the feasibility of performing multicolor interphase fluorescence in situ hybridization (FISH) on ThinPrep slides of transitional cell carcinoma (TCC).
  
  
  METHODS
  Slides from 20 voided urine specimens were prepared by the ThinPrep technique (Cytyc, Boxborough, Massachusetts, U.S.A.), pretreated using a pretreatment kit and subjected to hybridization with the multicolor FISH probe UroVysion (Vysis, Downers Grove, Illinois, U.S.A.). Archival slides were placed in xylene, destained in alcohol and washed prior to pretreatment. Urines from patients with cytology-positive, biopsy-proven grade 1 (n = 5), 2 (n = 7) and 3 (n = 5) TCC and negative cytology and biopsy (n = 3) were selected. Freshly prepared (n = 10) and archival (n = 10) slides were used.
  
  
  RESULTS
  All carcinoma cases were FISH positive (> 5 cells with complex abnormalities of > or = 2 studied chromosomes per slide). None of the normal samples were aneusomic. Gain of chromosomes 3, 7 and 17 constituted the majority of positive cases. Proper destaining and slight decrease in stringency wash conditions enabled reliable detection of signals in archival cases.
  
  
  CONCLUSIONS
  Routine ThinPrep slides can be used for multicolor interphase FISH analysis of urine cytology specimens. Archival slides provide the opportunity to analyze by FISH the nature of atypical cells identified by cytology. This revised method allows FISH technology more accessibility for routine use in cytology laboratories.
authors.affiliation: Department of Anatomic Pathology, Cleveland Clinic Foundation, Ohio 44195, USA. mskacel@pol.net
authors.forename: M
authors.initials: M
authors.lastname: Skacel
authors.forename: J D
authors.initials: JD
authors.lastname: Pettay
authors.forename: E K
authors.initials: EK
authors.lastname: Tsiftsakis
authors.forename: G W
authors.initials: GW
authors.lastname: Procop
authors.forename: C V
authors.initials: CV
authors.lastname: Biscotti
authors.forename: R R
authors.initials: RR
authors.lastname: Tubbs
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000473:pathology* / Q000652:urine; D003581:Cytodiagnosis / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008856:Microscopy, Fluorescence; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D001749:Urinary Bladder Neoplasms / Q000473:pathology* / Q000652:urine