Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications.
Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)-poly(anion) composite films, PANi-poly(vinylsulfonate) (PVS) or PANi-poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of beta-nicotinamide adenine dinucleotide (NAD(+)) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 degrees C.
Simon
Evelyne
E
Department of Chemistry, University of Southampton, Southampton SO17 1BJ, UK.
Halliwell
Catherine M
CM
Toh
Chee Seng
CS
Cass
Anthony E G
AE
Bartlett
Philip N
PN
D000814:Aniline Compounds; D000818:Animals; D000838:Anions; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000235:genetics / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000737:chemistry; D009243:NAD / Q000096:biosynthesis / Q000737:chemistry; D011108:Polymers; D011817:Rabbits
D000814:Aniline Compounds; D000838:Anions; D004800:Enzymes, Immobilized; D011108:Polymers; C416807:polyaniline; D009243:NAD; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase