title: Reduced sialidase expression in highly metastatic variants of mouse colon adenocarcinoma 26 and retardation of their metastatic ability by sialidase overexpression.
abstract: Sialidase expression levels are inversely correlated with the metastatic potential of mouse colon adenocarcinoma 26 sublines, as assessed by activity assays and RT-PCR, irrespective of total and cell surface sialic acid contents. Compared with low metastatic NL4 and NL44 cell lines, the highly metastatic NL17 and NL22 cells exhibit low expression of sialidases, accompanied with higher levels of sialylLe(x) and GM3. To investigate whether these properties of NL17 cells can be altered by sialidase overexpression, we transfected a cytosolic sialidase gene into NL17 cells. The result was markedly inhibited lung metastasis, invasion and cell motility with a concomitant decrease in sialylLe(x) and GM3 levels, in line with the case of spontaneously low metastatic sublines having relatively high endogenous sialidase levels, implying that sialidase level is a determining factor affecting metastatic ability. Treatment of the cells with antibodies against sialylLe(x) and GM3 affected cell adhesion and/or cell motility, providing evidence that desialylation of these molecules, as targets of sialidase, is involved in the suppression of metastasis.
authors.affiliation: Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, Medeshima-shiode, Natori, Miyagi 981-1293, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Sawada
authors.forename: Setsuko
authors.initials: S
authors.lastname: Moriya
authors.forename: Seiichi
authors.initials: S
authors.lastname: Saito
authors.forename: Ryuzaburo
authors.initials: R
authors.lastname: Shineha
authors.forename: Susumu
authors.initials: S
authors.lastname: Satomi
authors.forename: Takao
authors.initials: T
authors.lastname: Yamori
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuruo
authors.forename: Reiji
authors.initials: R
authors.lastname: Kannagi
authors.forename: Taeko
authors.initials: T
authors.lastname: Miyagi
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000556:secondary; D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry; D005679:G(M3) Ganglioside / Q000378:metabolism; D008175:Lung Neoplasms / Q000201:enzymology* / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009844:Oligosaccharides / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D005679:G(M3) Ganglioside; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases