Members of the Zyxin family of LIM proteins interact with members of the p130Cas family of signal transducers.
Integrin binding to extracellular matrix proteins induces formation of signaling complexes at focal adhesions. Zyxin co-localizes with integrins at sites of cell-substratum adhesion and is postulated to serve as a docking site for the assembly of multimeric protein complexes involved in regulating cell motility. Recently, we identified a new member of the zyxin family called TRIP6. TRIP6 is localized at focal adhesions and overexpression of TRIP6 slows cell migration. In an effort to define the molecular mechanism by which TRIP6 affects cell migration, the yeast two-hybrid assay was employed to identify proteins that directly bind to TRIP6. This assay revealed that both TRIP6 and zyxin interact with CasL/HEF1, a member of the Cas family. This association is mediated by the LIM region of the zyxin family members and the SH2 domain-binding region of CasL/HEF1. Furthermore, the association between p130(Cas) and the two zyxin family members was demonstrated to occur in vivo by co-immunoprecipitation. Zyxin and Cas family members may cooperate to regulate cell motility.
Yi
Jinseong
J
Huntsman Cancer Institute and Department of Biology, University of Utah, Salt Lake City, Utah 84112 and the University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Kloeker
Susanne
S
Jensen
Christopher C
CC
Bockholt
Susanne
S
Honda
Hiroaki
H
Hirai
Hisamura
H
Beckerle
Mary C
MC
D000074183:ATPases Associated with Diverse Cellular Activities; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D050719:Crk-Associated Substrate Protein; D060588:LIM Domain Proteins; D008667:Metalloproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011506:Proteins; D011817:Rabbits; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D050719:Crk-Associated Substrate Protein; D060588:LIM Domain Proteins; D008667:Metalloproteins; C099942:NEDD9 protein, human; C092282:PSMC5 protein, human; D010750:Phosphoproteins; D011506:Proteins; D050717:Retinoblastoma-Like Protein p130; D014157:Transcription Factors; D046988:Proteasome Endopeptidase Complex; D000074183:ATPases Associated with Diverse Cellular Activities