[Relative analysis of the mRNA expression between endothelin and inducible nitric oxide synthase in pulpitis].
OBJECTIVE
To study the relationship between endothelin-1(ET-1) and inducible nitric oxide synthase (iNOS) through the mRNA expression in pulpitis.


METHODS
The model of experimental rat pulpitis; extraction of RNA, the reverse-polymerase chain reaction(RT-PCR) were used.


RESULTS
The mRNA expression of ET-1/iNOS in pulpitis groups was higher than the control. There was relationship in the mRNA expression between ET-1 and iNOS.


CONCLUSIONS
Pulp microcirculation was mechanized by the contraction and relaxtion of ET-1/iNOS, which could have direct effects on the pathological change. The control mechanism was due to the up-regulation of ET-1/iNOS gene in transcription level.
Yan
Y
Y
School of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Lu
Z
Z
D000818:Animals; D019332:Endothelin-1 / Q000235:genetics*; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D011671:Pulpitis / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019332:Endothelin-1; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat