Absorption of a protein gavage in Zucker lean rats. Influence of protein content in the diet.
The rate of protein absorption was measured in Zucker lean rats. Rats were fed with a bolus that contained ca. 300 mg of 14C-labelled protein at the beginning of the light cycle. Blood was extracted from the portal vein at intervals up to 9 hours after gavage. Label incorporation into tissue protein was monitored. The digestion and absorption of protein was slow, and 9 hours after the gavage, 20% of the bolus remained in the stomach. Forty percent of the protein was absorbed in the first hour. This was followed first by a linear absorption process, then by the amino acid incorporation into tissue proteins. The appearance of label in the portal vein increased progressively for up to four hours, shifting to a progressive decrease that coincides with the maintenance of this label in the tissues. The skin, the striated muscle and the liver showed the highest amounts of labelled proteins. The application of this model to animals fed low-(LP) or high-protein (HP) content diets showed that the HP group digested the protein faster than the LP group, and that catabolism of the amino acids was higher in the HP group. The LP group digested protein much more slowly than the RD (control) group, but protein accretion was more efficient.
Masanés
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RM
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain.
Rafecas
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I
Remesar
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D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D002250:Carbon Radioisotopes; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion / Q000502:physiology; D007408:Intestinal Absorption / Q000502:physiology*; D008297:Male; D011506:Proteins / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D011924:Rats, Zucker
D000596:Amino Acids; D002250:Carbon Radioisotopes; D004044:Dietary Proteins; D011506:Proteins