Purification and distribution of ghrelin: the natural endogenous ligand for the growth hormone secretagogue receptor.
Growth hormone secretagogues (GHS) are small synthetic molecules that act through a specific G-protein coupled receptor (GPCR) termed GHS-R, which is a distinct receptor from that of growth hormone releasing hormone (GHRH). This receptor was one of the orphan GPCR, i.e. a GPCR with no known natural ligand, until we purified its endogenous ligand from rat stomach and named it "ghrelin". Ghrelin is a 28-amino-acid peptide, in which the serine 3 is modified by an n-octanoic acid and this modification is essential for ghrelin's activity. The discovery of ghrelin indicates that the release of GH from the pituitary may be regulated not only by hypothalamic GHRH, but also by ghrelin from the stomach and hypothalamus.
Kojima
M
M
Division of Molecular Genetics, Institute of Life Sciences, Kurume University, Kurume, Fukuoka 839-0861, Japan. mkojima@lsi.kurume-u.ac.jp
Hosoda
H
H
Kangawa
K
K
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D054439:Ghrelin; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D036361:Peptide Hormones; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D014018:Tissue Distribution
D054439:Ghrelin; D008024:Ligands; D036361:Peptide Hormones; D010455:Peptides; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin