Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis.
Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella.
Balasubramanian
Periasamy
P
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India.
Jayakumar
R
R
Shambharkar
Prashant
P
Unnamalai
N
N
Pandian
S Karutha
SK
Kumaraswami
N Selvamuthu
NS
Ilangovan
R
R
Sekar
Vaithilingam
V
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000378:metabolism*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity*; D001427:Bacterial Toxins; D001483:Base Sequence; D003001:Cloning, Molecular; D004175:Diptera / Q000187:drug effects; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000633:toxicity*; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins; D007915:Lepidoptera / Q000187:drug effects; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis