[Expression of cytokines in acute heart transplantation rejection].
OBJECTIVE
To observe the expression and changes of local cytokines network in rat heart transplantation so as to determine the role of cytokines in acute rejection of rat heart transplantation.


METHODS
The Wistar rats (receptor) were divided into four groups: group A(control, n = 12), group B(IL-2 Mab treated, n = 12), group C [Cyclosporine A (CsA) treated, n = 12] and group D(IL-2Mab + CsA treated, n = 12). Hearts from SD rats were transplanted into a cervical location in Wistar. The local expression of IL-1 beta, IL-2, CD25, IL-4, IL-5, IL-6, IL-10, TNF alpha, IFN gamma was examined at 1, 3, 5, 7, 9, 11, 14 days after operation by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Allografts survival time was (8.3 +/- 1.7) days in group A, (29.2 +/- 7.1) days in group B, (26.4 +/- 5.7) days in group C, (55.0 +/- 10.6) days in group D. The expression of IL-1 beta, IL-4, IL-10, IFN gamma was up-regulated, and the expression of IL-2, CD25, IL-5, IL-6, TNF alpha was significantly inhibited in group A. The expression of IL-1 beta, IL-5, IL-6, IL-10, IFN gamma was up-regulated, and the expression of IL-2, IL-4, TNF alpha was significantly down-regulated in group B. The expression of IL-1 beta, IL-2, CD25, IL-5, TNF alpha, IFN gamma was up-regulated, and the expression of IL-4, IL-6, Il-10 was significantly down-regulated in group C. The expression of IL-4, IL-5, IL-6, IL-10 was up-regulated, and the expression of IL-1 beta, IL-2, CD25, IFN gamma, TNF alpha was significantly down-regulated in group D.


CONCLUSIONS
Cytokines play an important role in the development of acute transplantational rejection. Different cytokines play different roles in different local environments; the main principle possibly is immune deviation.
Xia
J
J
Department of Cardiac Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Jiang
X
X
Huang
Y
Y
D000208:Acute Disease; D000818:Animals; D016207:Cytokines / Q000235:genetics* / Q000502:physiology; D006084:Graft Rejection / Q000209:etiology*; D016027:Heart Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines