Tranilast inhibits TGF- A1 secretion without affecting its mRNA levels in conjunctival cells.
The present study was conducted to assess the effect of Tranilast, a drug developed as anti-keloid and anti-hypertrophic scar agent, on the level of transforming growth factor- A1 (TGF- A1) mRNA, and on TGF- A1 secretion in Chang Conjunctiva cells. TGF- A1 mRNA was not detected in Chang Conjunctiva cells by Northern blot analysis, but reverse transcriptase-polymerase chain reaction (RT-PCR) analysis confirmed the presence of TGF- A1 mRNA. Tranilast, whereas the drug had no effect on the levels of TGF- A1 mRNA and cellular protein, time- and dose-dependently inhibited TGF- A1 secretion from Chang Conjunctiva cells in the enzyme-linked immunosorbent assay (ELISA) analysis. TGF- A1 is suggested to cause fibroblast proliferation, that obstructs aqueous humor filtration route after glaucoma filtration surgery. Tranilast, potentially inhibiting TGF- A1 secretion, therefore, could be a promising drug to prevent from scarring after glaucoma filtration surgery.
Ochiai
H
H
Shinnagata Eye Institute, 4-2-11 Udetsuka-cho, Nagata-ku, Kobe, 653-0036, Japan. ochiai@ganka.jp
Ochiai
Y
Y
Chihara
E
E
D000328:Adult; D002460:Cell Line; D003228:Conjunctiva / Q000166:cytology / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000235:genetics*; D053773:Transforming Growth Factor beta1; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D006634:Histamine H1 Antagonists; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D062367:ortho-Aminobenzoates; C012293:tranilast