title: Decreased affinity of PBP3 to methicillin in a clinical isolate of Staphylococcus epidermidis with borderline resistance to methicillin and free of the mecA gene.
abstract: Analysis of the antibiotic binding capacity of penicillin-binding proteins (PBPs) in a group of clinical isolates of Staphylococcus epidermidis suggests that the increased level of resistance to methicillin (MIC 4.0 microg/ml) in an isolate free of the mecA gene is due to the decreased binding capacity of PBP3.
authors.affiliation: Department of Microbiology, School of Medicine, University of Patras, Greece.
authors.forename: E
authors.initials: E
authors.lastname: Petinaki
authors.forename: G
authors.initials: G
authors.lastname: Dimitracopoulos
authors.forename: I
authors.initials: I
authors.lastname: Spiliopoulou
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D008566:Membranes / Q000378:metabolism; D008712:Methicillin / Q000378:metabolism*; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009097:Multienzyme Complexes / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013203:Staphylococcal Infections / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; C083616:mecA protein, Bacillus subtilis; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D008712:Methicillin