title: Microscopic overview of crinoid regeneration.
abstract: Crinoids are well known for their striking regenerative potential and can rapidly and completely regenerate arms lost following self-induced or traumatic amputation. Thus they provide a valuable experimental model for investigation of the regenerative process from the macroscopic to the molecular level. In these last years we have studied in detail the overall process of arm regeneration in the comatulid Antedon mediterranea. This phenomenon can be described on the whole as a typical blastemal regeneration in which new structures develop from migratory pluripotential, actively proliferating cells in the presence of presumptive regulatory factors. The overall process can be subdivided into three main phases: a repair phase, an early regenerative phase, and an advanced regenerative phase, whose crucial aspects are related to common fundamental mechanisms such as cell migration and proliferation, intervention of stem cells and/or dedifferentiated cells, contribution of putative growth factors, particularly in terms of specific neurally derived factors, and mechanisms of pattern formation. This article focuses on the main aspects of the phenomenon and gives a brief account of the most recent and relevant results. Our approach employs classical methods of light (LM) and electron (TEM and SEM) microscopy, immunocytochemistry, and histofluorescence on experimentally induced arm regenerations of standard or abnormal type obtained in significantly different experimental conditions, including extreme mutilations (explants) or exposure to pseudo-estrogenic environmental contamination.
authors.affiliation: Dipartimento di Biologia, Universita' degli Studi di Milano, 20133 Milano, Italy. cndmdn@mailserver.unimi.it
authors.forename: M D
authors.initials: MD
authors.lastname: Candia Carnevali
authors.forename: F
authors.initials: F
authors.lastname: Bonasoro
mesh_terms: D000818:Animals; D004447:Echinodermata / Q000033:anatomy & histology / Q000145:classification / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012038:Regeneration / Q000502:physiology