title: Role of p38 protein kinase in heat-induced Raw cells apoptosis.
abstract: OBJECTIVE
  To elucidate the role of p38 in heat-induced apoptosis in the monocytic cell line Raw264.7.
  
  
  METHODS
  Apoptosis was detected using flow cytometric analysis, DNA electrophoresis, Westero blot and a protein kinase assay.
  
  
  RESULTS
  A marked increased in p38 activity was detected 15 min after heating and reached maximal activity 30 min post stimulation, then returned to the pre-stimulated level 2 h later. The percentage of apoptotic cells was obviously increased 3 h after heating. Stimulation of p38 preceded the induction of apoptosis, and the p38 specific inhibitor, FHPI, can inhibit thermal injury induced cell apoptosis.
  
  
  CONCLUSIONS
  p38 activation is required for heat-induced apoptosis in Raw264.7 cells, and stimulation of the p38 signaling pathway contributes to cell death.
authors.affiliation: Key Laboratory for Shock and Microcirculation Research of PLA, Department of Pathophysiology, First Military Medical University, Guangzhou 510515, China. liuny@fimmu.edu.cn
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: K
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D004791:Enzyme Inhibitors; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases