Expression of bilirubin UDP-glucuronosyltransferase (bUGT) throughout fetal development: intrasplenic transplantation into Gunn rats to correct enzymatic deficiency.
The aim of this work was to determine the pattern of expression of hepatic bilirubin UDP-glucuronosyltransferase throughout fetal development in rats, with the purpose of using fetal hepatocytes at the most appropiate stage of development for transplantation into Gunn rats lacking bilirubin UDP-glucuronosyltransferase activity and then assessing the therapeutic capacity of the implants. The results show that at day 13 of gestational life there is already bilirubin UDP-glucuronosyltransferase gene expression. Twenty-one-day fetal hepatocyte transplantation was also performed into the spleens of hyperbilirubinemic Gunn rats, when alpha-fetoprotein mRNA is still detectable. At 15, 30, and 90 days after transplantation, a mild decrease in total bilirubin serum levels was observed. An increase in bile conjugated bilirubin also was observed at 30 and 90 days. These data suggest the favorable evolution of transplanted cells and show its feasibility for therapy.
Cubero
F J
FJ
Departamento de Biología Animal II, Facultad de Biología, UCM, Madrid, Spain.
Arza
E
E
Maganto
P
P
Barrutia
G
G
Mula
N
N
Ortiz
A
A
Arahuetes
R M
RM
D000818:Animals; D000831:Animals, Newborn; D001663:Bilirubin / Q000097:blood; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D020567:Fetal Weight; D015870:Gene Expression; D005865:Gestational Age; D014453:Glucuronosyltransferase / Q000378:metabolism*; D022781:Hepatocytes / Q000637:transplantation; D051381:Rats; D011911:Rats, Gunn; D017208:Rats, Wistar
C438610:UDP-glucuronosyltransferase 2B21; D014453:Glucuronosyltransferase; D001663:Bilirubin