title: [Analysis of glyphosate and its metabolite, aminomethylphosphonic acid, in agricultural products by HPLC].
abstract: An analytical method for residues of the herbicide glyphosate [GLY: N-(phosphonomethyl)-glycine] and its metabolite (AMPA: aminomethylphosphonic acid) in agricultural products is described. GLY and AMPA were extracted with water (soybeans extract was moreover treated with acetone), and purified by application to a dual mini-cartridge column (octadecyl-bonded silica gel and cation-exchange silica gel) system. GLY was derivatized with 9-fluorenylmethylchloroformate, and determined by HPLC with fluorescence detection. AMPA determination was performed by HPLC employing postcolumn fluorogenic labeling with o-phthalaldehyde-mercaptoethanol. The detection limits for GLY and AMPA were 0.04 microgram/g (soybean: 0.08 microgram/g), and both recoveries from samples to which the compounds were added at the 0.2 microgram/g level (soybean: 1.0 microgram/g) were more than 65%.
authors.affiliation: Saitama Institute of Public Health: 639-1, Kamiokubo, Saitama-shi, Saitama 338-0824, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Horie
authors.forename: N
authors.initials: N
authors.lastname: Aoba
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D013025:Glycine max / Q000737:chemistry; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D000097797:Glyphosate; C036423:1-aminomethylphosphonic acid