[The role of MHC molecules on OSCC cells in the induction of autologous cytotoxic T lymphocytes].
OBJECTIVE
To investigate the role of major histocompatibility complex(MHC) molecules in the induction of cytotoxic T lymphocyte(CTL) from oral squamous cell carcinoma.


METHODS
1. The treated autologous tumor cells served as stimulating cells, on which the MHC mAb was used to block the HLA-ABC and/or HLA-DR antigens. The specific cytotoxicity of CTL induced by stimulating cells and the level of released TNF-alpha were then detected. 2. By blocking the HLA-ABC and/or HLA-DR antigens on target cells, the inhibition effect of MHC mAb on CTL cytotoxicity induced by autologous stimulating cells (not treated with mAb) was measured.


RESULTS
1. The cytotoxicity induced by stimulating cells in experimental groups was significantly lower than that of the control group. The successive order was: the DR antigen positive stimulating cell group > ABC antigen positive stimulating cell group > both ABC and DR antigen negative stimulating cell group. The specific TNF-alpha released by CTL was completely blocked by anti-ABC mAb. 2. The average inhibition rate by mAb on cytotoxicity of CTL was: 52%, 27% and 72% by anti-ABC mAb, anti-DR mAb, and anti-ABC mAb plus anti-DR mAb respectively.


CONCLUSIONS
Both MHC-class I and class II antigens on OSCC cells play an important role in inducing CTL.
Yang
H
H
Department of Stomatology, Third Affiliated Hospital, Sun Yet-Sen University of Medical Sciences, Guangzhou 510630, China.
Li
J
J
Zhao
Y
Y
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D008285:Major Histocompatibility Complex / Q000276:immunology / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha