[Production and study of hybridomas, producing monoclonal antibodies to the structural glycoprotein of Marburg virus].
Hybridomas producing monoclonal antibodies (MAb) to Marburg virus glycoprotein were prepared by splicing of mouse myeloma cells (NSO and X.63-Ag6.653 strains) and splenocytes of immunized BALB/c mice. Cultural and MAb-producing properties of hybridomas were studied. The production of MAb during serial passages 15-30 was confirmed. MAb titers in culture fluids were 1:4096-1:8152, in immune ascitic fluids of BALB/c mice 1:409,600-1:638,400. The possibility of using MAbs as a component of ELISA test system was demonstrated. The sensitivity of ELISA-MAb method for Marburg virus was 1 x 10(3) PFU/mu.
Ruchko
S V
SV
Lebedev
V N
VN
Pashchenko
Iu I
IuI
Borisevich
G V
GV
Semenova
I S
IS
Khamitov
R A
RA
Maksimov
V A
VA
Firsova
I V
IV
Petrovskiĭ
A V
AV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology*; D029024:Marburgvirus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012692:Serial Passage; D014759:Viral Envelope Proteins / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; C081324:GP-protein, Marburg virus; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins
Poluchenie i izuchenie gibridom, produtsiruiushchikh monoklonal'nye antitela k strukturnomu glikoproteinu virusa Marburg.