Two-dimensional gel electrophoresis of selenized yeast and autoradiography of 75Se-containing proteins.
Two-dimensional high-resolution gel electrophoresis (2DE) has been applied to the fractionation of 75Se-containing proteins in yeast, grown in 75Se-containing medium, and autoradiography was used for detection of the 75Se-containing proteins. Gel filtration and ultrafiltration were used to check whether the selenium side-chains were stable in the presence of the chemicals used for lysis and 2DE. The mass distribution of the selenium-containing proteins was estimated by use of gel filtration and the results were compared with the distribution obtained by 2DE. A 2DE map of selenium-containing proteins in yeast is presented, and compared with a total protein map of yeast.
Chéry
C C
CC
Laboratory of Analytical Chemistry, Ghent University, Belgium. cyrille.chery@rug.ac.be
Dumont
E
E
Cornelis
R
R
Moens
L
L
D001345:Autoradiography; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D017279:Selenocysteine / Q000737:chemistry; D014462:Ultrafiltration
D005656:Fungal Proteins; D016566:Organoselenium Compounds; D017279:Selenocysteine