title: An investigation of immunocompetence substances in normal gingival and periodontitis tissue.
abstract: OBJECTIVE
  To investigate the effects of nitric oxide (NO), endothelin (ET), substance P (SP) and calcitonin gene-related peptide (CGRP) immunocompetence substances and their relationship to chronic periodontitis.
  
  
  METHODS
  Immunohistochemical aod histochemical staining methods were used to detect the expression of the NO synthase (NOS), ET, SP and CGRP levels in 20 patients with chronic periodontitis and 20 healthy subjects as control.
  
  
  RESULTS
  Quantitative analysis by Quantimat 970 showed that NOS and ET in periodontitis tissue increased significantly (P < 0.01), particularly the content of ET in comparison with healthy subjects. The intergroup expression of SP and CGRP showed no remarkable changes.
  
  
  CONCLUSIONS
  Our results demonstrate that the level of NOS and ET were significantly increased in periodontic tissue, which may diminish the blood supply and influence the periodontal tissue causing tissue damage. Our study suggests that immunocompetence substances NO and ET are closely associated with periodontitis and may play an important role in the disease.
authors.affiliation: Beijing Stomatological Hospital, Capital University of Medical Sciences, Beijing 100050, China.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000328:Adult; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis; D002908:Chronic Disease; D016232:Endothelins / Q000096:biosynthesis; D005260:Female; D005881:Gingiva / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis*; D010518:Periodontitis / Q000378:metabolism* / Q000473:pathology; D014176:Protein Biosynthesis; D013373:Substance P / Q000096:biosynthesis
chemical_list: D016232:Endothelins; D009569:Nitric Oxide; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide