title: Free radical-induced protein degradation of erythrocyte membrane is influenced by the localization of radical generation.
abstract: We have investigated the role of the localization of free radical generation in erythrocyte membrane proteins degradation. The extracellular radical producer 2,2'-azobis(2-amidinopropane) hydrochloride induced a greater loss of band-3 protein in comparison with spectrin whereas the intracellular radical initiator cysteine induced the reverse effect. However, a large extent of main-chain fragmentation was observed for both proteins under the action of cysteine-derived radicals. The results show that the relative localization of the radical generation has an important influence on the degradation of specific proteins of the erythrocyte membrane.
authors.affiliation: Instituto de Ciencias Biológicas y Químicas, Universidad de Valparaíso, Chile.
authors.forename: G
authors.initials: G
authors.lastname: Celedón
authors.forename: G
authors.initials: G
authors.lastname: González
authors.forename: E A
authors.initials: EA
authors.lastname: Lissi
authors.forename: G
authors.initials: G
authors.lastname: Hidalgo
mesh_terms: D000578:Amidines / Q000494:pharmacology; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D006868:Hydrolysis; D016877:Oxidants / Q000494:pharmacology; D013049:Spectrin / Q000378:metabolism*
chemical_list: D000578:Amidines; D001457:Anion Exchange Protein 1, Erythrocyte; D005609:Free Radicals; D016877:Oxidants; D013049:Spectrin; C046728:2,2'-azobis(2-amidinopropane); D003545:Cysteine