title: Arg485Lys polymorphism of factor V increases the risk of coronary artery disease in a Chinese population.
abstract: OBJECTIVE
  To explore the relationship between genetic variation in coagulation factor V and the occurrence of coronary arterial disease (CAD).
  
  
  METHODS
  Unrelated 86 patients with CAD and 102 healthy controls were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to detect variations in the entire twenty-five exons of the factor V gene.
  
  
  RESULTS
  Polymorphisms in exon 4 [642 G-->T (Ser156)], exon 10 [1628 G-->A (Arg485Lys)], exon 13 [4070 A-->G (His1299Arg)] and exon 16 [5380 G-->A (Val1736Met)] were documented. The study also identified a novel polymorphism in exon 2 (327 A-->G) which did not result in amino acid residue substitution. The Leiden mutation (Arg506Gln) was not detected in any of our 188 subjects. Among the 5 polymorphisms, the allele frequency of 1628 G-->A was significantly different between CAD patients and controls (0.69 vs 0.81, chi 2 = 6.908, P < 0.01). This is the first report of this finding in a Chinese population.
  
  
  CONCLUSIONS
  1628 G-->A polymorphism is associated with CAD and it may be a risk factor for CAD morbidity in the Chinese population.
authors.affiliation: Department of Cardiology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
authors.forename: W
authors.initials: W
authors.lastname: Le
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: R
authors.initials: R
authors.lastname: Tao
authors.forename: B
authors.initials: B
authors.lastname: You
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: W
authors.initials: W
authors.lastname: Cao
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.forename: R
authors.initials: R
authors.lastname: He
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Gong
mesh_terms: D000368:Aged; D003327:Coronary Disease / Q000209:etiology* / Q000235:genetics; D005165:Factor V / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk; D012307:Risk Factors
chemical_list: D005165:Factor V