[Effect of recombinant human bone morphogenetic protein-2 on the expression of osteopontin in human periodontal ligament cells].
OBJECTIVE
To investigate the effects of growth factor rhBMP-2 on the expression of osteopontin in periodontal ligament cells(PDLC).


METHODS
In culture model of human PDLC, in situ hybridization method was used to detect the expression of osteopontin in human PDLC.


RESULTS
The PDLC showed negative reaction to express osteopontin, while those PDLC stimulated by rhBMP-2 showed osteopontin mRNA positive reaction in plasm.


CONCLUSIONS
rhBMP-2 can induce the expression of osteopontin in PDLC. rhBMP-2 may be an effective growth factor in stimulating PDLC differentiation to osteoblast and promote periodontal regeneration.
Liu
H
H
Department of Periodontology and Oral Medicine, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Wang
Q
Q
Wu
Z
Z
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D053495:Osteopontin; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D053495:Osteopontin