[Establishment of in vitro serum-free embryo implantation model].
OBJECTIVE
To observe the growth and implantation of mouse embryos in vitro.


METHODS
In order to establish a serum-free coculture system, the whole components of decidual tissue was collected and cultured in vitro. Then 4-day-mouse embryos were added to the well-developed decidual cells, and the following changes of the above system were closely observed under microscope.


RESULTS
Mouse embryos were successfully hatched, adhered, implanted and outspreaded under the coculture system. The extraplacental cone and amniotic sac were formed as well. Decidual cells kept stable during the following 4-day coculture.


CONCLUSIONS
Decidual cell is an ideal feeder cell for coculture. Our results showed that the modified method of decidual cell culture and the establishment of implantation model were simple, practical and reliable.
Liu
J
J
Department of Obstetrics and Gynecology, Qingdao Municipal Hospital, Qingdao 266011, China.
Luo
L
L
Yan
J
J
D000818:Animals; D016895:Culture Media, Serum-Free; D010064:Embryo Implantation; D005314:Embryonic and Fetal Development; D005260:Female; D051379:Mice; D011247:Pregnancy
D016895:Culture Media, Serum-Free