Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films.
Immobilization of urease and glutamate dehydrogenase enzymes in electrochemically prepared polypyrrole-polyvinyl sulfonate films (PPY-PVS) was carried out using physical adsorption and electrochemical entrapment techniques. Detailed studies on optimum pH, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy of the enzymes in the immobilized state were conducted. The value of the apparent Michaelis-Menten constant was experimentally determined to be 2.5 and 2.7 for physically adsorbed and electrochemically entrapped urease in PPY-PVS films, respectively.
Gambhir
A
A
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India. anamikagulati@yahoo.com
Gerard
M
M
Mulchandani
A K
AK
Malhotra
B D
BD
D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D005969:Glutamate Dehydrogenase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013451:Sulfonic Acids / Q000737:chemistry*; D014510:Urease / Q000737:chemistry*
D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D013451:Sulfonic Acids; C008959:lyapolate; C067635:polypyrrole; D005969:Glutamate Dehydrogenase; D014510:Urease