title: N-linked oligosaccharides predominantly distribute in rat cerebellar cortex.
abstract: The distribution or localization of glycoconjugates in rat cerebellar cortex was investigated with 26 different kinds of lectins observed by light and electron microscopy. In paraffin-embedded tissues, PHA-L, PHA-E, DSA, WGA, ConA, LEA, LCA, PSA, and RCA-I, which mainly recognize N-linked oligosaccharide sugar structures, stained the cerebellar cortex, especially the molecular layer. PHA-L staining showed the highest selectivity for the molecular layer among these lectins. Pretreatment with N-glycanase altered the staining intensities of these lectins, whereas pretreatment with O-glycanase did not alter the intensity. In electron microscopy, the cell membrane and Golgi membranes of Purkinje cell, parallel fibers, and synaptic vesicles exhibited a positive reaction with PHA-L. Nuclear pores and synaptic vesicles were positive for WGA binding. These results suggest that there exist N-glycoside binding oligosaccharides predominantly in the cerebellar cortex, especially in the molecular layer, which sugar chains may be relevant to the synaptic transmission in the molecular layer.
authors.affiliation: Department of Neuropathology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8504, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyata
authors.forename: H
authors.initials: H
authors.lastname: Kawakami
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: H
authors.initials: H
authors.lastname: Hirano
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002525:Cerebellar Cortex / Q000737:chemistry*; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure; D006596:Hexosaminidases / Q000378:metabolism; D037102:Lectins / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D022022:Nuclear Pore / Q000737:chemistry / Q000648:ultrastructure; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011689:Purkinje Cells / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013572:Synaptic Vesicles / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D037102:Lectins; D006596:Hexosaminidases; C060638:glycopeptide alpha-N-acetylgalactosaminidase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase