title: Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa.
abstract: Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy.
authors.affiliation: Department of Surgery , Lund University, University Hospital Malmö, SE 20502 Malmö, Sweden. max.nystrom@swipnet.se
authors.forename: M
authors.initials: M
authors.lastname: Nyström
authors.forename: U P
authors.initials: UP
authors.lastname: Westin
authors.forename: C
authors.initials: C
authors.lastname: Linder
authors.forename: K
authors.initials: K
authors.lastname: Ohlsson
mesh_terms: D001706:Biopsy / Q000379:methods; D003106:Colon / Q000033:anatomy & histology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000502:physiology; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000378:metabolism; D008875:Middle Aged; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000378:metabolism*; D053481:Secretory Leukocyte Peptidase Inhibitor
chemical_list: D018836:Inflammation Mediators; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor