[The significance of CD3/MIB1 and CD3/CD25 in B cell lymphomas].
OBJECTIVE
To identify the distribution and significance of proliferating T cells (PTC) in normal tonsil and a group of B cell lymphomas.


METHODS
Double immunohistochemical staining was used to detect PTC with MIB1 expression. Giemsa staining was used to detect Helicobacter pylori (Hp) in gastric MALT-type lymphoma. Cell culture was applied to investigate the relationship among tumor cells, Hp and T cells.


RESULTS
Very few T cells expressed MIB1 in the follicular centre of normal tonsil and in that of follicular lymphoma. PTC were mainly distributed to the extrafollicular and T cell zones. So did CD3+ T cells expressed CD25. In low grade primary B cell gastric MALT-type lymphomas, tumor cells proliferated in vitro in response to Hp but died in the absence of T cells. PTC increased with increase in the degree of malignancy in different kinds of B cell lymphomas.


CONCLUSIONS
T cells in follicular centres of follicular lymphoma are not in cell cycle. Proliferation of neoplastic B cells in low grade gastric MALT-type lymphomas requires Hp-specific infiltrating T cells. Transformation from low to high grade lymphoma is associated with an increase in T cell activation. T cells expressing MIB1 might be related to prognosis.
Wang
J
J
Department of Pathology, Shanxi Tumour Hospital, Taiyuan 030013, China.
Zhang
J
J
Sun
Y
Y
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D009687:Nuclear Proteins; D015375:Receptors, Interleukin-2