[Kinetic method of tungsten analysis in tungsten-containing enzymes].
A highly sensitive method for tungsten detection in proteins based on the ability of this metal to catalyze the oxidation of with hydrogen peroxide is described. The method allows determining tungsten in protein samples in the concentration range of -0.05 to -0.4 microgram/ml. Molybdenum, at a concentration lower than half the concentration of tungsten, as well as iron, selenium, and pterin at concentrations -2.5 times higher than that of tungsten, had no effect on tungsten determination by this method.
Nosikov
A N
AN
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Chichikalo
E V
EV
L'vov
N P
NP
D006861:Hydrogen Peroxide / Q000737:chemistry; D007700:Kinetics; D009565:Nitrate Reductases / Q000737:chemistry*; D012248:Rhodotorula / Q000737:chemistry; D012680:Sensitivity and Specificity; D013854:Thioamides / Q000737:chemistry; D014414:Tungsten / Q000032:analysis*
D013854:Thioamides; D006861:Hydrogen Peroxide; D009565:Nitrate Reductases; C005728:rubeanic acid; D014414:Tungsten
Kineticheskiĭ metod opredeleniia vol'frama v vol'fram-soderzhashchikh fermentakh.