title: Method of determination of appropriate heat treatment of animal meal by immunoassay developed for detection of cooked beef: interlaboratory study.
abstract: An interlaboratory trial was conducted for the validation of an enzyme-linked immunosorbent assay (ELISA) method for determination of appropriate heat treatment of animal meal. A commercially available ELISA test kit developed for the identification of beef in cooked food was used in the study. Twelve laboratories from 7 European countries examined 2 different analytical protocols to establish the most appropriate analytical method. Three different samples were used, 2 animal waste materials sterilized at 129 and 134 degrees C (wet conditions), respectively, and a meat and bone meal material processed at dry conditions (maximum temperature, 140 degrees C). Statistical evaluation applying t-statistics showed that the animal meal treated according to European legislation (>133 degrees C) was clearly distinguishable from the 2 other test materials at a 99% confidence level using both analytical protocols. This method can be considered as a complementary test to the immunoassay developed for the detection of pork in cooked food that is already applied in routine analysis for the surveillance of rendering plants.
authors.affiliation: European Commission, Joint Research Centre, Food Products and Consumer Goods Unit, Ispra (VA), Italy.
authors.forename: E
authors.initials: E
authors.lastname: Björklund
authors.forename: L
authors.initials: L
authors.lastname: Pallaroni
authors.forename: C
authors.initials: C
authors.lastname: von Holst
authors.forename: W
authors.initials: W
authors.lastname: Unglaub
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005062:European Union; D005506:Food Contamination / Q000517:prevention & control; D006358:Hot Temperature; D007753:Laboratories; D008460:Meat / Q000032:analysis*