title: Chlamydial heat shock protein 60--specific T cells in inflamed salpingeal tissue.
abstract: OBJECTIVE
  To evaluate the role of chlamydial heat shock protein 60 (CHSP60)-specific T-lymphocytes in tubal factor infertility.
  
  
  METHODS
  Case series of patients with tubal factor infertility.
  
  
  METHODS
  Infertility Clinic, Department of Obstetrics and Gynecology, Helsinki University Central Hospital and Laboratory of Cell-Mediated Immunity, National Public Health Institute, Oulu, Finland.
  
  
  METHODS
  Five patients with tubal factor infertility who underwent elective salpingectomy because of hydrosalpinges.
  
  
  METHODS
  Collection of salpingeal tissue specimens for in vitro culture of T-lymphocytes.
  
  
  METHODS
  Cloning of Chlamydia trachomatis and CHSP60-specific T-lymphocyte lines derived from inflamed salpingeal tissue. Cytokine production analysis of the established T-lymphocyte clones.
  
  
  RESULTS
  Seventy-seven (34%) of the 229 T-lymphocyte clones recognized C. trachomatis and C. pneumoniae elementary bodies as target antigens. One-third of these Chlamydia genus-specific T-lymphocyte clones further recognized CHSP60 as the target antigen. Most of the CHSP60-specific T-lymphocyte clones produced predominantly IL-10.
  
  
  CONCLUSIONS
  CHSP60 may be an important T-lymphocyte antigen involved in the immunopathogenesis of tubal damage associated with chronic C. trachomatis infection.
authors.affiliation: National Public Health Institute, Oulu, Finland
authors.forename: Anne
authors.initials: A
authors.lastname: Kinnunen
authors.forename: Pontus
authors.initials: P
authors.lastname: Molander
authors.forename: Richard
authors.initials: R
authors.lastname: Morrison
authors.forename: Matti
authors.initials: M
authors.lastname: Lehtinen
authors.forename: Riitta
authors.initials: R
authors.lastname: Karttunen
authors.forename: Aila
authors.initials: A
authors.lastname: Tiitinen
authors.forename: Jorma
authors.initials: J
authors.lastname: Paavonen
authors.forename: Heljä Marja
authors.initials: HM
authors.lastname: Surcel
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000032:analysis*; D002690:Chlamydia Infections / Q000150:complications*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism; D005187:Fallopian Tubes / Q000382:microbiology*; D005260:Female; D005307:Fertilization in Vitro; D005656:Fungal Proteins; D006801:Humans; D007247:Infertility, Female / Q000209:etiology; D007249:Inflammation; D029701:Saccharomyces cerevisiae Proteins; D012488:Salpingitis / Q000503:physiopathology / Q000601:surgery*; D013601:T-Lymphocytes / Q000382:microbiology*; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: C092693:CTF18 protein, S cerevisiae; D018834:Chaperonin 60; D016207:Cytokines; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins