title: Comparison of different methods for the isolation of Burkholderia cepacia DNA from pure cultures and waste water.
abstract: DNA from Burkholderia cepacia was prepared from suspensions of pure cultures and artificially contaminated waste water. The efficacy of four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol extraction, microwave treatment, heat treatment) and six commercially available kits (Puregene, High Pure PCR Template Preparation Kit, InstaGene, QIAamp Tissue Kit, DNAzol, Elu-Quik) was compared in terms of sensitivity in a subsequent PCR. The results showed that a simple and inexpensive procedure using a lysis buffer containing proteinase K was superior to all other methods tested.
authors.affiliation: Institut für Mikrobiologie, Sanitätsakademie der Bundeswehr, Neuherbergstr. 11, 80937 München, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Merk
authors.forename: H
authors.initials: H
authors.lastname: Neubauer
authors.forename: H
authors.initials: H
authors.lastname: Meyer
authors.forename: I
authors.initials: I
authors.lastname: Greiser-Wilke
mesh_terms: D016956:Burkholderia cepacia / Q000235:genetics* / Q000302:isolation & purification; D018929:Cell Culture Techniques; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014865:Waste Disposal, Fluid; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D014873:Water Pollutants