Use of Taenia crassiceps cysticercus antigen preparations for detection of antibodies in cerebrospinal fluid samples from patients with neurocysticercosis (Taenia solium).
Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.
Pardini
Alessandra Xavier
AX
Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo SP. Brazil.
Peralta
Regina Helena
RH
Vaz
Adelaide José
AJ
Machado
Luis dos Ramos
Ldos R
Peralta
José Mauro
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D020019:Neurocysticercosis / Q000134:cerebrospinal fluid / Q000276:immunology*; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth