[Study of the radioprotective effect of the NO-synthase inhibitor L-NAME in Chinese hamster cell culture].
A radioprotective effect of L-NAME was estimated by the yield of the aberrant anaphases after exposure of Chinese hamster cells to different doses of gamma-rays and beta-particles. It was shown that cell treatment with L-NAME before irradiation only decreased the frequency of radiation-induced chromosome aberrations. The equal yield of the aberrant anaphases was found in the cells treated with L-NAME and irradiated with 6 Gy gamma-rays later and in the cells non-treated with L-NAME and irradiated with 3 Gy gamma-rays. The treatment of cells with L-NAME just before and immediately after irradiation did not modify the radiation-induced frequency of the chromosome aberrations. The treatment of cells with L-NAME decreased the level of SH-groups (as estimated by UV-spectrophotometer) and increased the chromatin condensation (as estimated by flow cytometry). It was suggested that radioprotective effect of L-NAVE may be connected with its cooperation with DNA. Condensation of L-NAME on the surface of DNA resulted in increasing of probability of the chemical repair of DNA-radicals after irradiation. Thus, these results indicate the involvement of NO-dependent mechanism of the realization of the radiation-induced damage to the hereditary cell structure and optimal conditions for the realization as well as the conceivable mechanism of radioprotective effect one of the most inhibitors of NO-synthase--L-NAME.
Gil'iano
N Ia
NIa
B.P. Konstantinov Institute of Nuclear Physics, Russian Academy of Science, St. Petersburg-Gatchina, 188350 Russia. giliano@omrb.pnpi.spb.ru
Bondarev
G N
GN
Bikineeva
E G
EG
Krasotskaia
G I
GI
Noskin
L A
LA
D000818:Animals; D001610:Beta Particles; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005720:Gamma Rays; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D004247:DNA; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester
Issledovanie radioprotektornogo éffekta ingibitora NO-sintazy L-NAME v kul'ture kletok kitaĭskogo khomiachka.