[Effect of composite salviae dropping pill on endothelin gene expression in circulating endothelial cells of patients with coronary heart disease].
OBJECTIVE
To explore the effect of composite salviae dropping pill (CSDP) on endothelin (ET-1) gene expression in circulating endothelial cells.


METHODS
Seventy cases of stable angina pectoris were randomly divided into 2 groups, the CSDP group and the isosorbide dinitrate (ID) group. They were treated with CSDP and ID respectively, their ET-1 gene expression in endothelial cells of peripheral circulation was measured before and after treatment by reverse transcriptase-Polymerase Chain Reaction and compared between the two groups as well as with that of 30 healthy subjects.


RESULTS
Electrophoresis banding of 546 bp cDNA procured from 69 cases of 70 patients was positive, while no positive banding was obtained from healthy subjects. Six cases from the 29 patients treated with CSDP had their banding turned to negative, while in the ID group, no one had turned to negative after treatment. And ET-1 PCR product (absorbed optic density) in the CSDP group was markedly lower than that in the ID group, P < 0.05.


CONCLUSIONS
DSP could inhibit ET-1 gene expression in endothelial cells of peripheral circulation directly.
Feng
P
P
Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006).
Qin
N
N
Qin
Y
Y
D000368:Aged; D000369:Aged, 80 and over; D003327:Coronary Disease / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts; D012333:RNA, Messenger / Q000235:genetics; D027561:Salvia miltiorrhiza
D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D010936:Plant Extracts; D012333:RNA, Messenger