High levels of RAE-1 isoforms on mouse tumor cell lines assessed by anti-"pan" RAE-1 antibody confer tumor susceptibility to NK cells.
Two sublines of the benzpyrene-induced mouse hepatoma cell line, G-1 and G-5, showed low and high metastatic ability, respectively, to the lung. We produced a polyclonal antibody (pAb) against RAE-1alpha. Five isoforms of RAE-1 have been identified to date, and this pAb recognized all isoforms and was named anti-"pan" RAE-1 pAb. The level of RAE-1 was approximately 5-fold higher in G-5 than in G-1, which was almost RAE-1-negative, as determined using anti-pan RAE-1 pAb. Expression levels of other markers including MHC class I (MHC-I) and Qa-1b were very low and indistinguishable in these sublines. NK-mediated cytotoxicity was determined with these sublines; G-5 was highly susceptible to NK-mediated cytolysis, while G-1 was relatively resistant. The NK-mediated G-5 > G-1 killing profile was diminished if the G-5 cells were pretreated with F(ab)(2)(') of anti-pan RAE-1 pAb. G-1, when transfected with Rae-1alpha cDNA, acquired NK-responsiveness similar to that of G-5. These and additional data using mouse cell lines with low MHC-I levels and various RAE-1 levels also demonstrated that RAE-1 level is critically associated with NK-susceptibility in tumor cells.
Masuda
Hisayo
H
Department of Immunology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka 537-8511, Japan.
Saeki
Yoshiko
Y
Nomura
Midori
M
Shida
Kyoko
K
Matsumoto
Misako
M
Ui
Michio
M
Lanier
Lewis L
LL
Seya
Tsukasa
T
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000378:metabolism; D001580:Benzopyrenes; D015153:Blotting, Western; D002273:Carcinogens; D018076:DNA, Complementary / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D005960:Glucosides / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008175:Lung Neoplasms / Q000473:pathology; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D009362:Neoplasm Metastasis; D017830:Octoxynol / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D020033:Protein Isoforms; D013154:Spleen / Q000166:cytology; D014162:Transfection; D014407:Tumor Cells, Cultured
D000906:Antibodies; D000911:Antibodies, Monoclonal; D001580:Benzopyrenes; D002273:Carcinogens; D018076:DNA, Complementary; D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; D020033:Protein Isoforms; C491219:Raet1a protein, mouse; C018619:octyl-beta-D-glucoside; D007371:Interferon-gamma; D017830:Octoxynol; D007770:L-Lactate Dehydrogenase