The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes.
The immobilization of the mutants of L-lactate dehydrogenase (LDH) on poly(aniline) (PANi) composite films has been investigated. Mutants possessing peptide tags of varying charge and nucleophilicity were created to probe the nature of the interaction between the protein and PANi. These results are significant for the development of a 'generic' approach to the immobilization of enzymes and other proteins.
Halliwell
Catherine M
CM
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK.
Simon
Evelyne
E
Toh
Chee Seng
CS
Cass
Anthony E G
AE
Bartlett
Philip N
PN
D001674:Bioelectric Energy Sources; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D010455:Peptides / Q000737:chemistry*
D004800:Enzymes, Immobilized; D010455:Peptides; D007770:L-Lactate Dehydrogenase