title: Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD.
abstract: We have developed a route for the synthesis of 2'-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.
authors.affiliation: Department of Chemistry, Brooklyn College, The City University of New York, 2900 Bedford Avenue, Brooklyn, New York 11210, USA.
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
authors.forename: Nicolas
authors.initials: N
authors.lastname: Carrasco
authors.forename: Marianna
authors.initials: M
authors.lastname: Teplova
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Wilds
authors.forename: Martin
authors.initials: M
authors.lastname: Egli
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D016566:Organoselenium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D009841:Oligonucleotides; D016566:Organoselenium Compounds; D012313:RNA; D004247:DNA