title: Truncated vitronectins: binding to immobilized fibrin and to fibrin clots, and their subsequent interaction with cells.
abstract: The plasminogen activator inhibitor-1 (PAI-1) is stabilized in its inhibitory conformation by binding to Vitronectin (Vn). The anchorage of PAI-1 to the fibrin fibers was recently shown to be mediated by Vn, and as such to modulate fibrinolysis. Here we report the mapping of the fibrin binding sites in Vn using truncated recombinant Vns, and show that two segments of Vn are involved: one at its carboxyl terminus (within residues 348-459) and one at its amino terminus (within residues 1-44). This mapping sets the stage for (i) the design of specific inhibitors for the Vn-fibrin interaction; (ii) for studying the role of this interaction in the anchoring of endothelial cells and platelets onto the fibrin clot; and (iii) for getting a deeper insight into the mechanism of the Vn-fibrin interaction in fibrinolysis. (c)2002 Elsevier Science.
authors.affiliation: Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, 76100, Israel.
authors.forename: Iris
authors.initials: I
authors.lastname: Schvartz
authors.forename: Dalia
authors.initials: D
authors.lastname: Seger
authors.forename: Galia
authors.initials: G
authors.lastname: Maik-Rachline
authors.forename: Tamar
authors.initials: T
authors.lastname: Kreizman
authors.forename: Shmuel
authors.initials: S
authors.lastname: Shaltiel
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D001792:Blood Platelets / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D019096:Vitronectin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D019096:Vitronectin; D005337:Fibrin