Preparation of sterol-imprinted polymers with the use of 2-(methacryloyloxy)ethyl phosphate.
Steroid-selective polymers were prepared by the molecular imprinting technique, using 2-(methacryloyloxy)ethyl phosphate as functional monomer. The retentivity and selectivity of the obtained imprinted polymers were evaluated by liquid chromatography. The cholesterol-imprinted polymer showed higher affinity for cholesterol than that for cholesterol derivatives. The selectivity of the imprinted polymer was superior to the imprinted polymer prepared with the conventional functional monomer, 2-(trifluoromethyl)acrylic acid. Estradiol was also imprinted and gave similar results, demonstrating that 2-(methacryloyloxy)ethyl phosphate would be suitable for imprinted polymers of cholesterol and related compounds.
Kugimiya
A
A
Laboratory of Synthetic Biochemistry, Faculty of Information Sciences, Hiroshima City University, Hiroshima, Japan.
Kuwada
Y
Y
Takeuchi
T
T
D002784:Cholesterol / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D008027:Light; D008689:Methacrylates / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
C454021:2-(methacryloyloxy)ethyl phosphate; D008689:Methacrylates; D011108:Polymers; D002784:Cholesterol