[Biosynthesis and use of coproporphyrins and uroporphyrins and their metal complexes in immune analysis and diagnostic methods].
Methods of synthesis of coproporphyrin and uroporphyrin by using bacteria of the genus Arthrobacter are proposed. Metal complexes of coproporphyrin and uroporphyrin with Pt, Pd, and Zn were synthesized. Their structures were identified by spectrophotometry, IR spectrometry, 1H-NMR, mass spectrometry, and HPLC. Data showing the possibility to use coproporphyrin III-metal complexes as luminophores for fluorescence detection of tumors. The current and prospective uses of metal complexes of water-soluble natural porphyrins in advanced immunofluorescence assays are discussed.
Bykhovskiĭ
V Ia
VIa
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Zaĭtseva
N I
NI
Mironov
A F
AF
Osin
N S
NS
Pecherskikh
E V
EV
Rumiantseva
V D
VD
Sukhin
G M
GM
D000818:Animals; D001173:Arthrobacter / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003306:Coproporphyrins / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008665:Metalloporphyrins / Q000138:chemical synthesis / Q000737:chemistry; D009369:Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D009928:Organ Specificity; D010165:Palladium; D010984:Platinum; D011424:Propionibacterium / Q000378:metabolism; D014578:Uroporphyrins / Q000737:chemistry / Q000378:metabolism; D015032:Zinc
D003306:Coproporphyrins; D003470:Culture Media; D005456:Fluorescent Dyes; D008665:Metalloporphyrins; D014578:Uroporphyrins; C026971:coproporphyrin III; C022181:uroporphyrin III; D010984:Platinum; D010165:Palladium; D015032:Zinc
Biosintez i primenenie koproporfirinov i uroporfirinov i ikh metallokompleksov v immnoanalize i diagnostike.