The cellular nuclear matrix regulatory protein YY1 does not directly interact with the mouse polyomavirus large tumor antigen.
The transcription factor Yin-Yang 1 (YY1) is a multifunctional protein involved in repressing and activating many promoters of cellular and viral genes. YY1 functions via protein-DNA but also protein-protein interactions. The latter has been documented between YY1 and early gene products of adenoviruses (Lewis et al., J. Virol. 69, 1628-1636 (1995)) and papillomaviruses (Lee et al., Virol. 72,4911-4917 (1998)). In this study, first of this kind on mouse polyomavirus (Py), we report that YY1 and the main viral regulatory protein, large tumor antigen (LT), do not interact directly in vivo. This evidence was obtained by use of two separate methods, immunoprecipitation (IP) and a yeast two-hybrid system.
Spanielová
H
H
Department of Genetics and Microbiology, Faculty of Natural Sciences, Charles University, Prague, Czech Republic. hs@natur.cuni.cz
Velková
K
K
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000276:immunology*; D050981:Erythroid-Specific DNA-Binding Factors; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D011233:Precipitin Tests; D014157:Transcription Factors / Q000276:immunology*; D020798:Two-Hybrid System Techniques; D050986:YY1 Transcription Factor
D000952:Antigens, Polyomavirus Transforming; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D009687:Nuclear Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494699:Yy1 protein, mouse