title: Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.
abstract: The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
authors.affiliation: Department of Animal Science, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Donna
authors.initials: D
authors.lastname: Kolber-Simonds
authors.forename: Kwang-Wook
authors.initials: KW
authors.lastname: Park
authors.forename: Hee-Tae
authors.initials: HT
authors.lastname: Cheong
authors.forename: Julia L
authors.initials: JL
authors.lastname: Greenstein
authors.forename: Gi-Sun
authors.initials: GS
authors.lastname: Im
authors.forename: Melissa
authors.initials: M
authors.lastname: Samuel
authors.forename: Aaron
authors.initials: A
authors.lastname: Bonk
authors.forename: August
authors.initials: A
authors.lastname: Rieke
authors.forename: Billy N
authors.initials: BN
authors.lastname: Day
authors.forename: Clifton N
authors.initials: CN
authors.lastname: Murphy
authors.forename: David B
authors.initials: DB
authors.lastname: Carter
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hawley
authors.forename: Randall S
authors.initials: RS
authors.lastname: Prather
mesh_terms: D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D019976:Cloning, Organism; D004624:Embryo Transfer; D005260:Female; D005333:Fetus; D005347:Fibroblasts; D005700:Galactosyltransferases / Q000235:genetics*; D018390:Gene Targeting; D005822:Genetic Vectors; D008297:Male; D016254:Mutagenesis, Insertional; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011995:Recombination, Genetic; D013552:Swine; D013556:Swine, Miniature / Q000196:embryology / Q000235:genetics*; D014162:Transfection
chemical_list: D005700:Galactosyltransferases; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase