Pulsatile activity and hatching of in vitro produced cow blastocysts: effects of serum supplementation.
The effect of serum added to a modified SOF medium on pulsatile activity and hatching of in vitro produced cow blastocysts was investigated by time-lapse cinematography. Embryos were generated from abattoir material and cultured in mSOF without serum or with 10% FCS added at 42h pi. Addition of serum significantly increases pulsatile activity before zona rupture and reduces the time of hatching. Pulsatile activity does not seem to be involved in the hatching process.
van Heule
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A
Université catholique de Louvain, Unité des sciences vétérinaire, Louvain-la-Neuve, Belgium.
van Langendonckt
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A
Donnay
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I
Dessy
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F
Massip
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A
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology*; D002417:Cattle / Q000196:embryology*; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005260:Female; D005312:Fetal Blood; D006801:Humans; D008297:Male; D018715:Microscopy, Video; D013223:Statistics as Topic; D013997:Time Factors; D015044:Zona Pellucida / Q000502:physiology
D003470:Culture Media