[Interleukin-1 increase nitric oxide synthesis through up-regulation of inducible nitric-oxide synthase by rabbit mandibular condylar cartilage cells in vitro].
OBJECTIVE
To investigate the influence of IL-1 on the biosynthesis of nitric oxide by rabbit mandibular condylar cartilage (MCC) cells.


METHODS
MCC cells of white rabbits were harvested enzymatically and cultured in DMEM supplemented with 0.4% newborn calf serum. In the experiment, a variety of concentrations of IL-1 were added to the medium. NO production was determined as nitrite accumulation in the medium. The effects of IL-1 on the mRNA levels of inducible nitric-oxide synthase (iNOS) were assessed by mRNA in situ hybridization method.


RESULTS
IL-1 can significantly increase nitric oxide synthesis by MCC cells in a concentration-dependent manner. In addition, normal MCC cells had light expression of iNOS expression and IL-1 (10-100 micrograms/L) promoted the expression of iNOS.


CONCLUSIONS
IL-1 can inhibit the chondrocytes proliferation and induce apoptosis that might be through the way of up-regulation of iNOS expression.
Jiao
Y
Y
Diagnostic and Treatment Center for Temporomandibular Disorders, Peking University School of Stomatology, Beijing 100081, China.
Ma
X
X
Zhang
Z
Z
D000818:Animals; D002356:Cartilage / Q000166:cytology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008335:Mandibular Condyle / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D011817:Rabbits; D015854:Up-Regulation
D007375:Interleukin-1; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II