The catalytic mechanism of nucleoside diphosphate kinases.
Nucleoside diphosphate kinases catalyze the reversible transfer of the gamma phosphate of nucleoside triphosphates to nucleoside diphosphates. This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. We also briefly discuss the physiological relevance of in vitro studies.
Lascu
I
I
Institut de Biochimie et Génétique Cellulaires, UMR 5095 University of Bordeaux-2 and CNRS, France. ioan.lascu@ibgc.u-bordeaux2.fr
Gonin
P
P
D000818:Animals; D002384:Catalysis; D002413:Cations, Divalent; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008670:Metals; D009701:Nucleoside-Diphosphate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D013379:Substrate Specificity
D002413:Cations, Divalent; D004791:Enzyme Inhibitors; D008670:Metals; D009701:Nucleoside-Diphosphate Kinase