title: [Retroviral transduction of a mutant erbB-2 gene into human CD34+ derived dendritic cells].
abstract: OBJECTIVE
  To successfully transduce a mutant erbB-2 gene into normal human CD34(+)-derived dendritic cells (DCs) and verify gene expression in these transduced dendritic cells.
  
  
  METHODS
  The packaging cell line PA317 was transfected with mutant erbB-2 gene DNA and the virus produced was used to infect packaging cell line PG13. The virus produced by PG13 was used to infect CD34(+)-derived dendritic cells by the spinoculation method using flasks coated with fibronectin material to facilitate retrovirus gene transter efficiency and the mutant erbB-2 gene expression was assessed by ABC staining and FACScan methods.
  
  
  RESULTS
  A mutant erbB-2 gene packaging cell line was produced and this mutant gene was transduced into human CD34(+)-derived DCs. It was verified that the relatively large numbers of the transduced DCs expressed the mutant erbB-2 protein which was eradicated of the ability to transform mouse NIH3T3 fibroblast cells.
  
  
  CONCLUSIONS
  Human DCs can be gene-modified and these gene-modified DCs may be useful in stimulating T lymphocytes for immunotherapy.
authors.affiliation: Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078, China.
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Hong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001943:Breast Neoplasms / Q000628:therapy; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D022761:Gene Transfer, Horizontal; D018734:Genes, erbB-2; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D009154:Mutation; D012190:Retroviridae / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured