[Preclinical study of preimplantation genetic diagnosis in animal].
OBJECTIVE
To perform preimplantation genetic diagnosis (PGD) in transgenic mice.


METHODS
Single blastomere from murine heterozygous pre-embryo at 4 or 8-cell stage was sampled by microbiopsy for detection of the human gene delta F508 mutation of cystic fibrosis and biopsied pre-embryo was transferred to Swiss mice foster mothers. delta F508 mutation detection on the single blastomere was performed with nested polymerase chain reaction(PCR).


RESULTS
(1) Significant difference was not observed statistically for the in vitro hatching rate (n = 102), the birth rate (n = 79), birth weight and organ weight (n = 133) at 3 weeks after births between mice born from biopsied embryos and controls. (2) Thirty-two nested PCR were performed for the diagnosis of the cystic fibrosis delta F508 mutation, with 100.0% specificity and 100.0% sensitivity.


CONCLUSIONS
Microbiopsy of pre-embryos with 4 or 8-cells did not alter their viability and further development. The technique of nested PCR on a single blastomere for the detection of cystic fibrosis delta F508 mutation is reliable.
Ji
Y
Y
Center of Reproductive Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhao
Y
Y
Sheng
Y
Y
D000818:Animals; D003951:Diagnostic Errors; D004624:Embryo Transfer; D005260:Female; D051379:Mice; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D019836:Preimplantation Diagnosis