[The relationship between hypoxic pulmonary hypertension and changes of HO-1 and its mRNA].
OBJECTIVE
To study the relationship of HO-1 and HPH.


METHODS
The expression of HO-1 and HO-1 mRNA were observed in pulmonary vessles of rats using the technique of immunohistochemistry and hybridization in situ. The average value of integral light density of HO-1 in three different diameters of arteries was detected by means of the image analysor and considered as the relative content in pulmonary arteries.


RESULTS
Hypoxia induced the expression of HO-1 and HO-1 mRNA and the relative content of HO-1 was significantly increased in pulmonary arteries corresponding with the period of hypoxia.


CONCLUSIONS
In the condition of HPH, relative content of HO-1 increase obviously. It suggests that HO-1 plays certain role in development of HPH. The change of HO-1 mRNA expression is the basis of molecular change of HO-1.
Yang
Z
Z
304th Hospital PLA, Beijing 100037.
Liang
Y
Y
Lu
J
J
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D051547:Heme Oxygenase-1; D006976:Hypertension, Pulmonary / Q000378:metabolism* / Q000473:pathology; D000860:Hypoxia / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008168:Lung / Q000378:metabolism / Q000473:pathology; D011651:Pulmonary Artery / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1