Dengue virus replicative intermediate RNA detection by reverse transcription-PCR.
Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.
Vaughan
Gilberto
G
Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Carpio 470, Col. Santo Tomas, México DF 11340, México.
Olivera
Hiram
H
Santos-Argumedo
Leopoldo
L
Landa
Abraham
A
Briseño
Baltasar
B
Escobar-Gutiérrez
Alejandro
A
D000818:Animals; D002460:Cell Line; D009033:Culicidae; D003716:Dengue Virus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
D012367:RNA, Viral