[hCG-PLZF-RARA transgenic mice develop leukemia resembling human chronic myeloid leukemia].
OBJECTIVE
In order to investigate the leukemogenic potential of PLZF-RARA fusion protein in vivo, hCG-PLZF-RARA transgenic mice were generated, in which PIZF-RARA fusion gene was driven by hCG promoter to express in myeloid cells of mice.


METHODS
Molecular cloning technology was used to construct hCG-PLZF-RARA gene. The genotype and phenotype of the hCG-PLZF-RARA transgenic mice were analyzed by PCR, RT-PCR, immunofluorescence, morphology of bone marrow (BM) cells, pathology and retinoic acid differentiation assays.


RESULTS
Six hCG-PLZF-RARA transgenic mice developed leukemia resembling human chronic myeloid leukemia. TF(tissue factor) was not expressed in BM cells of normal mice nor in mice without the expressed transgene, but it was expressed in mice expessing the transgene.


CONCLUSIONS
PLZF-RARA fusion protein plays a crucial role in leukemogenesis. TF is up-regulated by PLZF-RARA fusion gene.
Meng
X
X
Shanghai Hematology Research Institute, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Cheng
G
G
Wang
L
L
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000235:genetics*; D004195:Disease Models, Animal; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D006063:Chorionic Gonadotropin; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; C118318:PLZF-RARalpha fusion protein, human; C492209:PLZF-RARalpha fusion protein, mouse; D011993:Recombinant Fusion Proteins; D013925:Thromboplastin