title: Aminoguanidine renders inducible nitric oxide synthase knockout mice more susceptible to Salmonella typhimurium infection.
abstract: Aminoguanidine (AG), a nitric oxide synthase (NOS) inhibitor, has been widely used to study the role of inducible NOS (iNOS) in host defense against infections caused by various pathogens including Salmonella typhimurium. iNOS has been reported to play an important role in host defense against S. typhimurium infection both in vitro and in vivo. In this report we show those AG treatment lead to weight loss in both wild-type and iNOS knockout mice, and rendered them more susceptible to Salmonella infection. These results suggest that AG may have side effects other than the inhibition of iNOS, and that data obtained from studies using AG should be interpreted with caution.
authors.affiliation: Department of Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, PA, USA. xinzhou81@hotmail.com
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Potoka
authors.forename: Patricia
authors.initials: P
authors.lastname: Boyle
authors.forename: Evan P
authors.initials: EP
authors.lastname: Nadler
authors.forename: Kathleen
authors.initials: K
authors.lastname: McGinnis
authors.forename: Henri R
authors.initials: HR
authors.lastname: Ford
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000008:administration & dosage*; D006146:Guanidines / Q000008:administration & dosage*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000401:mortality*; D012486:Salmonella typhimurium / Q000276:immunology*
chemical_list: D004791:Enzyme Inhibitors; D006146:Guanidines; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C004479:pimagedine