title: [Criteria for evaluation of immunoblots using Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu stricto for diagnosis of Lyme borreliosis].
abstract: The immunoblot was prepared from genotypes Borrelia afzelii (KC 90), Borrelia garinii (M 192) and Borrelia burgdorferi sensu stricto (B 31). Sera of 63 patients with different forms of Lyme borreliosis were examined and 40 healthy donors in the endemic area of the disease. In class IgM in the group of patients significantly more frequently antibodies against OspC, p39, p41 B. afzelii, p39, p41, p66, p83 B. garinii and OspC1, OspA, B. burgdorferi sensu stricto were found. In class IgG there were antibodies against p39, p41, p93 B. afzelii, p14, p41, p93 B. garinii and OspA, OspC p93 B. burgdorferi sensu lato. Based on the assembled results by means of discrimination analysis and logistic regression the most suitable combinations of antigens for evaluation of immunoblots in different genotypes were determined. Furthermore evaluation was suggested using a combination of antigens of several genotypes which led to an increased sensitivity and specificity of the immunoblot. Tables were prepared for easier evaluation of newly examined sera samples.
authors.affiliation: Infekcní klinika FN, Hradec Králové.
authors.forename: K
authors.initials: K
authors.lastname: Honegr
authors.forename: J
authors.initials: J
authors.lastname: Havlasová
authors.forename: P
authors.initials: P
authors.lastname: Gebouský
authors.forename: V
authors.initials: V
authors.lastname: Dostál
authors.forename: V
authors.initials: V
authors.lastname: Pellantová
authors.forename: Z
authors.initials: Z
authors.lastname: Skrabková
authors.forename: D
authors.initials: D
authors.lastname: Hulínská
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D016015:Logistic Models; D008193:Lyme Disease / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M
vernacular_title: Kritéria hodnocení imunoblotu v diagnostice lysmké borreliózy pri pouzití Borrelia afzelii, Borrelia garinii a Borrelia burgdorferi sensu stricto.