Immobilization of lactate dehydrogenase on tetraethylorthosilicate-derived sol-gel films for application to lactate biosensor.
Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mM L-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4-10 degrees C.
Chaubey
A
A
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India. achaubey@csnpl.ren.nic.in
Gerard
M
M
Singh
V S
VS
Malhotra
B D
BD
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D011817:Rabbits; D012821:Silanes / Q000737:chemistry*; D013696:Temperature; D014466:Ultraviolet Rays
D020099:Coated Materials, Biocompatible; D005782:Gels; D012821:Silanes; D019344:Lactic Acid; C040733:tetraethoxysilane; D007770:L-Lactate Dehydrogenase