Generation of influenza A virus from cloned cDNAs--historical perspective and outlook for the new millenium.
Influenza virus reverse genetics has reached a level of sophistication where one can confidently generate virus entirely from cloned DNAs. The new systems makes it feasible to study the molecular mechanisms of virus replication and pathogenicity, as well as to generate attenuated live virus vaccines, gene delivery vehicles, and possibly other RNA viruses from cloned cDNAs. During the next decade, one can anticipate the translation of influenza virus reverse genetics into biomedically relevant advances.
Neumann
Gabriele
G
Department of Pathobiological Sciences, School of Veterinary edicine, University of Wisconsin, Madison, WI, USA.
Kawaoka
Yoshihiro
Y
D000818:Animals; D018076:DNA, Complementary; D004279:DNA, Viral; D006378:Helper Viruses; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D007252:Influenza Vaccines; D016296:Mutagenesis; D012261:Ribonucleoproteins / Q000502:physiology; D014158:Transcription, Genetic; D014613:Vaccines, Attenuated; D014779:Virus Replication
D018076:DNA, Complementary; D004279:DNA, Viral; D007252:Influenza Vaccines; D012261:Ribonucleoproteins; D014613:Vaccines, Attenuated