[Modulation of multidrug resistance by 1-phenyl-2-palmitoylamino-3-morpholino-1-propanol in SKOV3-adriamycin-resistant cell line].
OBJECTIVE
To study the modulation of mdr1 and P-glycoprotein (P-gp) by 1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) in SKOV3-adriamycin-resistant (SKOV3/AdrR) cell line.


METHODS
SKOV3/AdrR cells were treated with PPMP, mRNA expression of multidrug resistant (mdr1) gene was analyzed by reverse transcriptase-polymerase chain reaction. Intracellular rhodamine (Rh123) concentration was measured by flow cytometry.


RESULTS
PPMP was found to inhibit mdr1 expression of SKOV3/AdrR at the mRNA level. This modulation of gene expression was content dependent and complete inhibition appeared at 25 mumol/L PPMP treatment. PPMP could increase intracellular Rh123 accumulation in SKOV3/AdrR cells. After 15, 25 mumol/L PPMP treatment, Rh123 accumulation in SKOV3/AdrR was markedly enhanced Rh123 fluorescence intensity were 389.98, 426.08 respectively (P < 0.05).


CONCLUSIONS
PPMP could modulate mdr1 expression at the mRNA level in a content dependent manner. P-gp mediated outward efflux of cytotoxic agents can be blocked by PPMP in SKOV3/AdrR cell. PPMP possesses multi-drug resistance activity in ovarian cancer.
Yuan
Y
Y
Department of Obstetrics and Gynecology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Wang
L
L
Zhang
J
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009025:Morpholines / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D013107:Sphingolipids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C095823:1-phenyl-2-palmitoylamino-3-morpholino-1-propanol; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004791:Enzyme Inhibitors; D009025:Morpholines; D012333:RNA, Messenger; D013107:Sphingolipids; D004317:Doxorubicin