[Application of sequential culture in assisted reproductive technique].
OBJECTIVE
To investigate the effects of sequential culture technique on the outcomes of in vitro fertilization-embryo transfer(IVF-ET).


METHODS
Sequential culture media were used in 47 IVF-ET cycles, and conventional medium in 114 cycles. The fertilization rate, cleavage rate and speed, good embryo rate and pregnancy rate were compared between these 2 groups.


RESULTS
There were significantly higher embryo cleavage and quality score rate, pregnancy rate (97.9%, 64.4%, 46.7%) in sequential media group as compared those in conventional medium group (89.7%, 40.2%, 28.2%).


CONCLUSIONS
Sequential culture system is more suitable for the development of cultured human oocytes and embryos.
Sun
M
M
Center of Reproductive Medicine, Shandong Province Hospital, Jinan 250021, China.
Feng
Y
Y
Gao
Q
Q
D001757:Blastomeres; D003470:Culture Media; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D006801:Humans; D027724:Reproductive Techniques, Assisted
D003470:Culture Media