title: Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot.
abstract: A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foot rot as regards morphology, enzymic profile and 16S rDNA sequence. On the basis of 16S rDNA, it was most closely related to another unnamed spirochaete isolated from cases of bovine digital dermatitis in the USA, raising the possibility of cross-species transmission. Further information is required to establish this novel ovine spirochaete as the cause of SVOFR.
authors.forename: I
authors.initials: I
authors.lastname: Demirkan
authors.forename: S D
authors.initials: SD
authors.lastname: Carter
authors.affiliation: Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Winstanley
authors.affiliation: Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
authors.forename: K D
authors.initials: KD
authors.lastname: Bruce
authors.affiliation: Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
authors.forename: N M
authors.initials: NM
authors.lastname: McNAIR
authors.affiliation: Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Woodside
authors.affiliation: Departments of Veterinary Immunology and *Medical Microbiology and Genitourinary Medicine, University of Liverpool, Liverpool, †Division of Life Sciences, Franklin-Wilkins Building, Kings College, London, ‡Veterinary Research Laboratory, Belfast, Northern Ireland and §Clare Veterinary Group, Ballyclare, Northern Ireland.
authors.forename: C A
authors.initials: CA
authors.lastname: Hart
mesh_terms: D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D005534:Foot Diseases / Q000382:microbiology / Q000662:veterinary*; D005535:Foot Rot / Q000382:microbiology*; D006724:Hoof and Claw / Q000382:microbiology / Q000473:pathology; D008854:Microscopy, Electron / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D014208:Treponema / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D014211:Treponemal Infections / Q000382:microbiology / Q000662:veterinary*; D014774:Virulence
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S