[Linked marker analysis of bronchial asthma in China].
OBJECTIVE
To study the clinical application value of genetic linkage analysis for bronchial asthma with two STRs-D5S436 and D5S658.


METHODS
Polymorphism of the two STRs were amplified by polymerase chain reaction, and the alleles identification were performed with denaturing polyacrylamide gel electrophoresis and silver-staining techniques.


RESULTS
In 92 unrelated Chinese individuals, 8 alleles and 22 phenotypes in D5S436, 9 alleles and 28 phenotypes in D5S658 were observed. The heterozygosities were 78% and 73% respectively. The polymorphism information contents (PIC) were 0.76 and 0.80 respectively. Linkage analysis showed linkage of asthma related phenotypes (including asthma) with D5S436 on chromosome 5q (lod = 2.490, theta = 0.1).


CONCLUSIONS
D5S436 and D5S658 were good genetic markers. Linkage analysis demonstrates that locus on chromosome 5q31-33 maybe was linked to the development of asthma in Chinese population.
Huang
K
K
Beijing Red Cross Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020.
Liu
J
J
Weng
X
X
D000293:Adolescent; D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D001249:Asthma / Q000235:genetics*; D001980:Bronchi; D002681:China; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods
D005819:Genetic Markers