title: Use of one-bottle adhesive as an intermediate bonding layer to reduce sealant microleakage on saliva-contaminated enamel.
abstract: OBJECTIVE
  To evaluate the influence of three different adhesives, each used as an intermediary layer, on microleakage of sealants applied under condition of salivary contamination.
  
  
  METHODS
  Six different experimental conditions were compared, 3 with adhesives and 3 without. After prophylaxis and acid etching of enamel, salivary contamination was placed for 10 s. In Group SC the sealant was applied after saliva without bonding agent and then light-cured. In Group SCA, after saliva, the surface was air dried, and then the sealant was applied and cured. In Groups ScB, SB and PB, a bonding agent (Scotchbond Dual Cure/3M, Single Bond/3M and Prime & Bond 2.1/Dentsply, respectively) was applied after the saliva and prior to the sealant application and curing. After storage in distilled water at 37 degrees C for 24 hrs, the teeth were submitted to 500 thermal cycles (5 degrees C and 55 degrees C), and silver nitrate was used as a leakage tracer. Leakage data were collected on cross sections as percentage of total enamel-sealant interface length. Representative samples were evaluated under SEM.
  
  
  RESULTS
  Sealants placed on contaminated enamel with no bonding agent showed extensive microleakage (94.27% in SC; 42.65% in SCA). The SEM revealed gaps as wide as 20 microm in areas where silver nitrate leakage could be visualized. In contrast, all bonding agent groups showed leakage less than 6.9%. Placement of sealant with a dentin-bonding agent on contaminated enamel significantly reduced microleakage (P < 0.0001). The use of a bonding agent as an intermediary layer between enamel and sealant significantly reduced saliva's effect on sealant microleakage.
authors.affiliation: Department of Pediatric Dentistry, São Paulo State University, Araraquara, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Hebling
authors.forename: R J
authors.initials: RJ
authors.lastname: Feigal
mesh_terms: D000134:Acid Etching, Dental; D000269:Adhesives / Q000737:chemistry; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D001840:Dental Bonding; D003743:Dental Enamel / Q000648:ultrastructure*; D003763:Dental Leakage / Q000473:pathology / Q000517:prevention & control*; D003777:Dental Prophylaxis; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D012463:Saliva; D016622:Silver Staining; D013223:Statistics as Topic; D013499:Surface Properties; D013816:Thermodynamics; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000269:Adhesives; D017220:Dentin-Bonding Agents; D010895:Pit and Fissure Sealants; D011109:Polymethacrylic Acids; C103500:Prime & Bond; D019279:Resin Cements; C115612:single bond; D014867:Water; C074318:Scotchbond Dual Cure; D017438:Bisphenol A-Glycidyl Methacrylate