title: Proliferative activity of benign and neoplastic endocervical epithelium and correlation with HPV DNA detection.
abstract: Recent studies have indicated that the use of the MIB-1 immunostaining may be useful in distinguishing endocervical neoplasia from benign nonneoplastic lesions. We sought to investigate this finding further with a specific emphasis on the common benign processes that may result in a nonspecific increase of MIB-1 staining. In this study we quantified the MIB-1 immunostaining in the mucinous endocervical epithelium (n=45) and in tubal metaplasia (n=28) during the proliferative and secretory phases (hormonal influence), in the mucinous endocervical epithelium in cases of cervicitis (inflammation) (n=10), in cases with a history of a recent biopsy (regeneration) (n=15), endocervical polyps (benign growth) (n=8), in the endocervical glands adjacent to a squamous intraepithelial lesion (human papilloma virus [HPV] infection) (n=63), and in in situ and invasive cervical adenocarcinomas (n=30). All cases with increased MIB-1 staining were subsequently tested for the presence of HPV DNA. The range of MIB-1 staining in the benign endocervical epithelium was from 0% to 48% and in the neoplastic epithelium from 25% to 84%. MIB-1 staining below 10% always reflected a benign process and MIB-1 staining higher than 50% was always associated with a neoplasia. Rare benign cases (tubal metaplasia during the proliferative phase, glands adjacent to squamous intraepithelial lesions, and cases with a history of a recent biopsy) had increased MIB-1 index, which overlapped with the neoplastic cases. In conclusion, MIB-1 is a useful marker of endocervical neoplasia, although in rare cases an overlap between benign and neoplastic cases may exist.
authors.affiliation: Department of Pathology, Weill Medical College of Cornell University, New York, New York 10021, USA.
authors.forename: Edyta C
authors.initials: EC
authors.lastname: Pirog
authors.forename: Christina
authors.initials: C
authors.lastname: Isacson
authors.forename: Mathias J
authors.initials: MJ
authors.lastname: Szabolcs
authors.forename: Bernhard
authors.initials: B
authors.lastname: Kleter
authors.forename: Wim
authors.initials: W
authors.lastname: Quint
authors.forename: Ralph M
authors.initials: RM
authors.lastname: Richart
mesh_terms: D034961:Antigens, Nuclear; D002278:Carcinoma in Situ / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008597:Menstrual Cycle; D009687:Nuclear Proteins / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D016133:Polymerase Chain Reaction; D011127:Polyps / Q000473:pathology / Q000821:virology; D014412:Tumor Virus Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D002575:Uterine Cervicitis / Q000473:pathology / Q000821:virology
chemical_list: D034961:Antigens, Nuclear; D004279:DNA, Viral; D019394:Ki-67 Antigen; D009687:Nuclear Proteins