[Changes in [Ca2+]i and IP3 levels in the process of cisplatin-induced apoptosis of gastric carcinoma].
OBJECTIVE
To study the change and significance of intracellular-free calcium levels ([Ca2+]i) and inositol 1, 4, 5-trisphosphate (IP3) levels in the process of gastric carcinoma cell apoptosis induced by cisplatin.


METHODS
Apoptosis induced by cisplatin in gastric carcinoma BGC823 cell was investigated by light and electronmicroscopy, agarose gel electrophoresis and flow cytometry. [Ca2+]i was determined by Fura-2 fluorescin load technic, IP3 was determined by competitive protein binding method.


RESULTS
When BGC823 cells were treated with cisplatin (2 micrograms/ml) for 24 hours changes appeared typical of apoptosis. [Ca2+]i and IP3 were significantly increased, especially at the initial stage of apoptosis. However, from 2 to 24 hours after cisplatin treatment, IP3 levels progressively decreased, being significantly lower than those of the untreated controls.


CONCLUSIONS
In the process of cisplatin induced apoptosis of gastric carcinoma cells, [Ca2+]i and IP3 levels are up-regulated at the very initial stage but down-regulated from 2 hours afterwards.
Xing
C
C
Department of Oncology, First Affiliated Hospital, China Medical University, Shenyang 110001.
Chen
J
J
Xu
H
H
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000032:analysis*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D015544:Inositol 1,4,5-Trisphosphate; D002945:Cisplatin; D002118:Calcium