title: GL7 defines the cycling stage of pre-B cells in murine bone marrow.
abstract: We have identified a novel subset of early B lineage cells in the mouse bone marrow (BM) by GL7 expression on cell surface. GL7(+)B220(low) BM cells have a large cell size and are CD43(-to low), CD95(-), Sca-1(-), I-A(low), IgM(-) and IgD(-), suggesting that they are large pre-B cells. These BM cells express lambda5 and VpreB but not terminal deoxytransferase (TdT) and Bcl-2, and approximately 50 % of them are in cell cycle. This fraction was not detected in BM cells of Rag-1-deficient and Scid mice, supporting that GL7(+)B220(low) BM cells belong to fraction C' and D according to Hardy's criteria or to an early large pre-B-II fraction according to Melchers-Rolink's criteria. Furthermore, GL7(+)B220(low) BM cells can differentiate into IgM(+) immature B cells in co-culture with stromal cells. These results suggest that B lymphocytes pass through the GL7(+) pre-B cell stage during differentiation in the BM. Thus, GL7 is the critical marker to define the proliferation stage of large pre-B cells.
authors.affiliation: Department of Developmental Genetics, Graduate School of Medicine, Chiba University, Chiba, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Murasawa
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Shintaro
authors.initials: S
authors.lastname: Obata
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hatano
authors.forename: Hideshige
authors.initials: H
authors.lastname: Moriya
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tokuhisa
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000502:physiology; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000276:immunology* / Q000502:physiology; D002453:Cell Cycle; D002454:Cell Differentiation; D019070:Cell Lineage; D018858:Germinal Center; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000502:physiology; D007075:Immunoglobulin M / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID
chemical_list: D000943:Antigens, Differentiation; D015415:Biomarkers; D007075:Immunoglobulin M; C096712:antigen GL7; D017493:Leukocyte Common Antigens