title: Post transcriptional control of gene expression in Leishmania.
abstract: Leishmania parasites are ancient eukaryotes, characterized by unusual molecular mechanisms. We have used the gene encoding for Hsp83 as a model system for studying regulatory mechanisms that control developmental gene regulation. We previously showed that protein coding genes are regulated exclusively by post-transcriptional mechanisms, while no transcriptional activation could be observed even for the conserved Hsp83 gene. We now show that processing and maturation of the Hsp83 polycistronic primary transcripts is more efficient at elevated temperatures. The mature transcripts are more stable during heat shock, with regulation conferred by 3' UTRs. Poly(A) tails of Hsp83 are approximately 30 nucleotides long, as common for other low eukaryotes. The mechanism that signals differential degradation is still unclear, since it was not possible to detect differences in deadenylation of Hsp83 transcripts at varying temperatures. Heat shock transcripts are preferentially translated at 33-37 degrees C, but unlike Drosophila, translational regulation is controlled by a region within the 3' UTR. Using this traditionally conserved system emphasizes that regulatory mechanisms in Leishmania differ from those prevailing in other eukaryotes.
authors.affiliation: Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel. shapiram@bgumail.bgu.ac.il
authors.forename: M
authors.initials: M
authors.lastname: Shapira
authors.forename: A
authors.initials: A
authors.lastname: Zilka
authors.forename: S
authors.initials: S
authors.lastname: Garlapati
authors.forename: E
authors.initials: E
authors.lastname: Dahan
authors.forename: I
authors.initials: I
authors.lastname: Dahan
authors.forename: V
authors.initials: V
authors.lastname: Yavesky
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D007891:Leishmania / Q000235:genetics* / Q000378:metabolism; D026723:Polyadenylation; D015800:Protozoan Proteins; D012323:RNA Processing, Post-Transcriptional
chemical_list: D020413:3' Untranslated Regions; D006360:Heat-Shock Proteins; C490310:Hsp83 protein, protozoan; D015800:Protozoan Proteins