Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle.
OBJECTIVE
To investigate the gene expression at transcription level of growth factor Wnt-5A in different phase during the cell cycle.


METHODS
We synchronized the renal cell carcinoma GRC-1 cell line by double thymidine blocks and high-pressure N2O gae methods and amplified Wnt-5A cDNAs from different phase using Semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction). The PCR products were electrophoresized on the agrose gel and detected by Gel Doc 1000 computer controlled system integrating the volumes of each band, representing the intensities of all pixels in a defined band.


RESULTS
The different mRNA expressions of growth factor Wnt-5A was detected in RCC GRC-1 cell line. In S phase, the highest level of Wnt-5A transcript was observed, and in G1 and M phase, medial and lowest, respectively. The differences between S and M stages were statistically significant (P < 0.05).


CONCLUSIONS
Growth factor Wnt-5A has the potential effect on tumorigenesis. It contributes to all phases during cell cycle but in S phase especially.
Zhuang
L
L
Urological Department, First Hospital, Institute of Urology, Beijing Medical University, Beijing 100034, China.
Guo
H
H
Xin
D
D
Zhang
Z
Z
Li
H
H
Yuan
X
X
Tang
D
D
Ding
Y
Y
Liu
L
L
Guo
Y
Y
D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein