[Mechanisms of drug resistance of primary renal cell carcinomas and their clinical significance].
OBJECTIVE
By multifactorial analysis, to study the mechanisms of drug resistance of primary renal cell carcinomas (RCC).


METHODS
Seventy cases of paraffin-embedded RCC tissue sections were examined for the expression of P-gp, GST-pi and Topo II by immunohistochemical staining.


RESULTS
There was 65.7% of RCC with expressed P-gp. Its Expression was stronger in late than in early stage of RCC. GST-pi was expressed in 41.1% RCC, but the magnitude of expression did not correlate with differentiation, type and stage of RCC. All the RCC examined had Topo II expression. Its expression level was lower than that of cancer of the intestine and lung. Among the 70 cases of RCC, 31.4% of them expressed at least one of the 3 drug-resistance parameters. Co-expression of two drug-resistance parameters was observed in 42.8% of the cases that of 3 drug-resistauce parameters in 22.9% of the cases.


CONCLUSIONS
Drug resistance is of common occurrence in RCC. To achieve better therapeutic efficacy, appropriate chemotherapeutic agents should be given to patients with low expression of P-gp and GST-pi but high expression of Topo II.
Zhang
Q
Q
Department of Urology, Fujian Provincial Hospital, Fuzhou 350001, China.
Chen
Z
Z
Shi
Z
Z
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D002648:Child; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004351:Drug Resistance / Q000502:physiology*; D018432:Drug Resistance, Multiple / Q000502:physiology; D005260:Female; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007680:Kidney Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D004250:DNA Topoisomerases, Type II