Salt stress and hyperosmotic stress regulate the expression of different sets of genes in Synechocystis sp. PCC 6803.
Acclimation of microorganisms to environmental stress is closely related to the expression of various genes. We report here that salt stress and hyperosmotic stress have different effects on the cytoplasmic volume and gene expression in Synechocystis sp. PCC 6803. DNA microarray analysis indicated that salt stress strongly induced the genes for some ribosomal proteins. Hyperosmotic stress strongly induced the genes for 3-ketoacyl-acyl carrier protein reductase and rare lipoprotein A. Genes whose expression was induced both by salt stress and by hyperosmotic stress included those for heat-shock proteins and the enzymes for the synthesis of glucosylglycerol. We also found that each kind of stress induced a number of genes for proteins of unknown function. Our findings suggest that Synechocystis recognizes salt stress and hyperosmotic stress as different stimuli, although mechanisms common to the responses to each form of stress might also contribute to gene expression.
Kanesaki
Yu
Y
Department of Regulation Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Suzuki
Iwane
I
Allakhverdiev
Suleyman I
SI
Mikami
Koji
K
Murata
Norio
N
D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis; D000458:Cyanobacteria / Q000235:genetics* / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D005960:Glucosides / Q000096:biosynthesis; D017270:Lipoprotein(a) / Q000096:biosynthesis; D020411:Oligonucleotide Array Sequence Analysis; D009997:Osmotic Pressure; D012492:Salts / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation
D005960:Glucosides; D017270:Lipoprotein(a); D012492:Salts; C034727:glucosylglycerol; D012965:Sodium Chloride; D013012:Sorbitol; D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase