[The effects of basic fibroblast growth factor on proliferation and differentiation of human dental pulp cells in vitro].
OBJECTIVE
To evaluate the effects of human basic fibroblast growth factor (hbFGF) on proliferation and differentiation of human dental pulp cells in vitro.


METHODS
The syntheses of DNA, collagen, fibronection (FN), alkaline phosphatase (ALP) and bone morphogenetic protein (BMP), and the expression of agglutinin were measured with imagine processing and analysis system.


RESULTS
hbFGF at concentration of 1-10 micrograms/L stimulated the cell proliferation measured by MTT colorimetric assay, and promoted incorporation of 3H-thymidine at 1-100 micrograms/L. The expression of type I collagen, FN, Con A receptor, and ALP significantly increased at hbFGF concentration of 10-1,000 micrograms/L, but the expression of type III collagen and WGA receptor markedly decreased at the same concentration. There was no significant difference in expression of BMP.


CONCLUSIONS
bFGF has the capability to promote the differentiation of dental pulp cells to odontoblasts in culture.
Hao
J
J
School of Stomatology, Fourth Military Medical University, Xi'an 710032.
Wang
P
P
Shi
J
J
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005353:Fibronectins / Q000096:biosynthesis; D006801:Humans; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D019485:Bone Morphogenetic Proteins; D024042:Collagen Type I; D005353:Fibronectins; D014316:Tritium; D016222:Fibroblast Growth Factor 2; D004247:DNA; D000469:Alkaline Phosphatase; D013936:Thymidine