Automated flow-injection spectrophotometric determination of nitrosamines in solid food samples.
A spectrophotometric method using a flow-injection system is proposed for the determination of nitrosamines in foods (cured-meats). The method is based on the photochemical cleavage of the N-NO bond of the nitrosamine to yield the corresponding amine and nitrite. The nitrite is then detected spectrophotometrically, at 542 nm, by use of its reaction with a modified Griess reagent. Different linear ranges between 0.8 and 2000 ng mL(-1) are obtained for the method, depending on the time of exposure of the sample to the UV radiation. The relative standard deviation varies between 2.0 and 3.9% and the sample throughput is between three and seven samples per hour, depending on the experimental conditions. The proposed method was successfully applied to the analysis of N-nitrosodimethylamine in cured ham and loin, bacon, and sausages.
Luque-Pérez
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E
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Ríos
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A
Valcárcel
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M
D000818:Animals; D002273:Carcinogens / Q000032:analysis*; D017022:Flow Injection Analysis; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D002273:Carcinogens; D007202:Indicators and Reagents; D009602:Nitrosamines