[Mechanisms of bone repairment in periapical diseases: studies on adjusting bone metabolism with calcium hydroxide in vitro].
OBJECTIVE
To provide experiment evidence for clinical practice and for further exploration on mechanism and medicinal control of periapical diseases.


METHODS
The culture model of neonatal mouse calvariae which has the similar biological activity to alveolar bone was established in vitro to observe the influence of calcium hydroxide on osteo-activity.


RESULTS
The results showed that calcium hydroxide with proper concentration at organ culture level could enhance osteoblast ALP secreting activity, and increased the tendency of bone formation. At the same time, the concentration of inorganic PO4(3-) in culture medium also had significant changes.


CONCLUSIONS
The mechanism of calcium hydroxide promoting repairment of bone tissues is not only by providing rich Ca2+ and alkaline environment mineral deposition, but also by stimulating calcification enzyme activity of osteoblast. Meanwhile, increased PO4(3-) concentration might improve the mineralization environment, which causes mineralization easily.
Liang
Y
Y
Department of Operative Dentistry and Endodontics, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Wang
J
J
Yu
S
S
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000187:drug effects / Q000503:physiopathology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D051379:Mice; D009924:Organ Culture Techniques; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology; D010483:Periapical Diseases / Q000188:drug therapy / Q000503:physiopathology*; D010710:Phosphates / Q000494:pharmacology; D012886:Skull / Q000187:drug effects / Q000254:growth & development; D013997:Time Factors
D010710:Phosphates; D000469:Alkaline Phosphatase; D002126:Calcium Hydroxide