The Escherichia coli ftsK1 mutation attenuates the induction of sigma(S)-dependent genes upon transition to stationary phase.
A mutation in the cell division gene ftsK causes super-induction of sigma(70)-dependent stress defense genes, such as uspA, during entry of cells into stationary phase. In contrast, we report here that stationary phase induction of sigma(S)-dependent genes, uspB and cfa, is attenuated and that sigma(S) accumulates at a lower rate in ftsK1 cells. Ectopic overexpression of rpoS restored induction of the rpoS regulon in the ftsK mutant, as did a deletion in the recA gene. Thus, a mutation in the cell division gene, ftsK, uncouples the otherwise coordinated induction of sigma(S)-dependent genes and the universal stress response gene, uspA, during entry into stationary phase.
Diez
Alfredo A
AA
Department of Cell and Molecular Biology-Microbiology, Göteborg, Sweden.
Tunlid
Anders
A
Nyström
Thomas
T
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003521:Cyclopropanes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D029968:Escherichia coli Proteins; D005227:Fatty Acids / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D003521:Cyclopropanes; D029968:Escherichia coli Proteins; D005227:Fatty Acids; C096452:FtsK protein, E coli; D008565:Membrane Proteins; D012808:Sigma Factor; C028775:cyclopropane fatty acids; C069567:sigma factor KatF protein, Bacteria; C030797:cyclopropane; D011938:Rec A Recombinases