[Interspecies conjugation of Escherichia coli-Streptomyces globisporus 1912 using integrative plasmid pSET152 and its derivatives].
Streptomyces globisporus 1912 produces a novel angucycline antitumor antibiotic landomycin E (LE). To study the LE biosynthetic gene cluster in detail, a system for the conjugal transfer of the integrative plasmid pSET152 from Escherichia coli into S. globisporus 1912 has been developed. It was shown that this plasmid integrates into two sites of the S. globisporus chromosome and is stably inherited under nonselective conditions. pSET152+ exconjugants of the strain 1912 are characterized by a significant decrease in LE synthesis (by 50-90%). A negative effect of pSET152 integration on antibiotic production was observed even upon the use of the recipient strain with increased LE synthesis, although in this case, the level of LE production in ex-conjugants was 120-150% of that in the original strain 1912. Based on pSET152, a vector system for gene knockouts in S. globisporus was developed. The effectivity of this system was shown in the example of disruption of the lndA gene encoding the key enzyme of LE synthesis (beta-ketoacylsynthase). Inactivation of this gene was shown to lead to the cessation of LE biosynthesis.
Luzhetskiĭ
A N
AN
Department Genetics and Biotechnology, Ivan Franko National University of Lviv, 79005 Ukraine.
Ostash
B E
BE
Fedorenko
V A
VA
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005810:Multigene Family; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C113593:landomycin E
Mezhrodovaia kon''iugatsiia Escherichia coli-Streptomyces globisporus 1912 s ispol'zovaniem integrativnoĭ plazmidy pSET152 i ee proizvodnykh.