Mannose-receptor positive and negative mouse macrophages differ in their susceptibility to infection by Chlamydia species.
It has been shown that N-linked high mannose type oligosaccharides competitively inhibits attachment to and infectivity of chlamydiae in HeLa cells. To further study whether mannose moieties are involved in the infectivity of chlamydiae, the susceptibility of mannose-receptor negative J774A and positive J774E mouse macrophages to Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae was evaluated. C. trachomatis infected mannose-receptor positive cells better than mannose-receptor negative cells. C. psittaci infected both mannose-receptor negative and positive cells equally well, while C. pneumoniae infected mannose-receptor negative cells better than mannose-receptor positive cells. Further studies using this system may provide insight into the role of mannose-receptor in attachment, entry and survival of chlamydiae in macrophages.
Kuo
Cho-chou
CC
Department of Pathobiology, University of Washington, Seattle, Washington, Washington 98195, USA. cckuo@u.washington.edu
Puolakkainen
Mirja
M
Lin
Tsun-Mei
TM
Witte
Marcia
M
Campbell
Lee Ann
LA
D000818:Animals; D002460:Cell Line; D002689:Chlamydia / Q000145:classification / Q000472:pathogenicity*; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D037181:Lectins, C-Type; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014774:Virulence
D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface