title: (CA/TG) microsatellite sequences escape the inhibition of recombination by mismatch repair in Saccharomyces cerevisiae.
abstract: Sequence divergence reduces the frequency of recombination, a process that is dependent on the activity of the mismatch repair system. In the yeast Saccharomyces cerevisiae, repair of mismatches results in gene conversion or restoration, whereas failure to repair mismatches results in postmeiotic segregation (PMS). By examining the conversion and PMS in yeast strains deficient in various MMR genes and heterozygous for large inserts (107 bp) with either a mixed sequence or a 39 (CA/TG) repetitive microsatellite sequence, we demonstrate that: (1) the inhibition of conversion by large inserts depends upon a complex containing both Msh2 and Pms1 proteins; (2) conversion is not inhibited if the single-stranded DNA loop in the heteroduplex is the microsatellite sequence; and (3) large heteroduplex loops with random sequence or repetitive sequence might be repaired by two complexes, containing either Msh2 or Pms1. Our results suggest that inhibition of recombination by heterologous inserts and large loop repair are not processed by the same MMR complexes. We propose that the inhibition of conversion by large inserts is due to recognition by the Msh2/Pms1 complex of mismatches created by intrastrand interactions in the heteroduplex loop.
authors.affiliation: UMR-CNRS 2027, Institut Curie-section de Recherche, Université Paris-Sud, F-91405 Orsay, France.
authors.forename: C G
authors.initials: CG
authors.lastname: Gendrel
authors.forename: M
authors.initials: M
authors.lastname: Dutreix
mesh_terms: D020137:Base Pair Mismatch; D002352:Carrier Proteins / Q000235:genetics; D003434:Crossing Over, Genetic; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics; D004171:Diploidy; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006579:Heterozygote; D008540:Meiosis; D018895:Microsatellite Repeats; D008957:Models, Genetic; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein; D009690:Nucleic Acid Conformation; D020161:Physical Chromosome Mapping; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C088858:PMS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C489751:MSH2 protein, S cerevisiae; D000070956:MutL Proteins; D051718:MutS Homolog 2 Protein