title: Assessment of dietary exposure to ochratoxin A in the UK using a duplicate diet approach and analysis of urine and plasma samples.
abstract: The approach to assess exposure to ochratoxin A from the diet by the analysis of human plasma and urine samples has been developed. Composite duplicate diet samples from 50 individuals and corresponding plasma and urine samples were obtained over 30 days. Samples were analysed using sensitive methods capable of measuring ochratoxin A at 0.001 ng g(-1) in food, 0.1 ng ml(-1) in plasma and 0.01 ng ml(-1) in urine. Analysis of the foods indicated ochratoxin A levels contributing to an average intake in the range 0.26-3.54 ng kg(-1) bw day(-1) over the 30 days. Ochratoxin A was found in all plasma samples and in 46 urine samples. The correlation between the plasma ochratoxin A levels and ochratoxin A consumption was not significant (95% confidence limit). However, a significant correlation was found between ochratoxin A consumption and the urine ochratoxin A concentration expressed as the total amount excreted. This new work offers the possibility of using ochratoxin A in urine as a simple and reliable biomarker to estimate exposure to this mycotoxin.
authors.affiliation: Department for Environment, Food and Rural Affairs, Central Science Laboratory, Sand Hutton, York, UK. j.gilbert@csl.gov.uk
authors.forename: J
authors.initials: J
authors.lastname: Gilbert
authors.forename: P
authors.initials: P
authors.lastname: Brereton
authors.forename: S
authors.initials: S
authors.lastname: MacDonald
mesh_terms: D000328:Adult; D000367:Age Factors; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002274:Carcinogens, Environmental / Q000032:analysis*; D015930:Diet Records; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008297:Male; D009793:Ochratoxins / Q000097:blood* / Q000652:urine; D012737:Sex Factors; D006113:United Kingdom
chemical_list: D015415:Biomarkers; D002274:Carcinogens, Environmental; D009793:Ochratoxins; C025589:ochratoxin A