title: Pulp response to direct capping with an adhesive system.
abstract: OBJECTIVE
  To evaluate the pulp response following direct pulp capping with an adhesive system (Prime & Bond 2.0 - PB 2.0) and a zinc-oxide eugenol cement (ZOE) on pulp exposures in rat molar teeth.
  
  
  METHODS
  Forty-eight Class I cavities were prepared on the occlusal surface of molar teeth of rats (Rattus Norvegicus, Holtzman). Pulp exposures performed on the cavity floor were capped either with the adhesive system P&B 2.0 or ZOE. After 7, 15, 30, and 60 days, the specimens were processed through H & E and Brown & Brenn staining techniques.
  
  
  RESULTS
  Both pulp capping materials allowed pulp repair, characterized by reorganization of a new odontoblast cell layer underlying the dentin bridge formation. However, P&B 2.0 promoted a large zone of cell-rich fibrodentin matrix deposition between the pulp capping material and the dentin bridge, which was deposited far from the pulp exposure site. On the other hand, pulps capped with ZOE showed dentin bridging immediately subjacent to the pulp capping material. In those samples in which microleakage occurred between dental material and cavity walls there was a persistent inflammatory reaction and lack of complete pulp repair.
authors.affiliation: Department of Oral Pathology, School of Dentistry, University of Michigan, Ann Arbor 48109-1078, USA. hebcosta@umich.edu
authors.forename: C A
authors.initials: CA
authors.lastname: Costa
authors.forename: A N
authors.initials: AN
authors.lastname: Mesas
authors.forename: J
authors.initials: J
authors.lastname: Hebling
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000627:therapeutic use; D004396:Coloring Agents; D003188:Composite Resins; D003737:Dental Cavity Preparation / Q000145:classification; D003763:Dental Leakage / Q000473:pathology; D003782:Dental Pulp / Q000187:drug effects* / Q000503:physiopathology; D003785:Dental Pulp Capping; D003789:Dental Pulp Exposure / Q000188:drug therapy / Q000503:physiopathology; D003793:Dental Restoration, Permanent; D003809:Dentin, Secondary / Q000187:drug effects / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000627:therapeutic use*; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D008297:Male; D008963:Molar; D009804:Odontoblasts / Q000187:drug effects / Q000502:physiology; D011109:Polymethacrylic Acids / Q000627:therapeutic use; D011671:Pulpitis / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D013499:Surface Properties; D014945:Wound Healing / Q000187:drug effects; D015035:Zinc Oxide-Eugenol Cement / Q000627:therapeutic use
chemical_list: D004396:Coloring Agents; D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D011109:Polymethacrylic Acids; C103500:Prime & Bond; D015035:Zinc Oxide-Eugenol Cement; D017438:Bisphenol A-Glycidyl Methacrylate