title: Effect of aloe-emodin on expression of proliferating cell nuclear antigen of vascular smooth muscle cells in culture after arterial injury.
abstract: OBJECTIVE
  To observe the effect of aloe-emodin on the expression of proliferating cell nuclear antigen (PCNA) in smooth muscle cells (SMCs) after arterial injury and study the molecular mechanism of inhibition of aloe-emodin on SMC proliferation.
  
  
  METHODS
  Deendothelialization was performed at the abdominal aorta in Japanese white rabbits using a 3F Fogarty arterial embolectomy catheter. 48 hours later, the medium of abdominal aorta was isolated and primary SMCs culture was performed. Cells were synchronized to G0 by serum starvation, then aloe-emodin at a concentration of 20 micrograms/ml was added to the culture medium containing 10% [v/v] fetal calf serum. Vehicle was also added to the medium as a control. After 18 hours, the expression of PCNA at the level of mRNA and protein were examined using techniques of RT/PCR, Western blotting and inmmunocytochemistry respectively.
  
  
  RESULTS
  Compared with the control group, the expression of PCNA mRNA and protein was prominently decreased after addition of aloe-emodin.
  
  
  CONCLUSIONS
  The inhibition of aloe-emodin on SMCs proliferation may be caused by inhibiting the expression of the PCNA gene.
authors.affiliation: Department of Cardiology, People's Hospital, Beijing Medical University, Beijing 100044, China.
authors.forename: C
authors.initials: C
authors.lastname: Xu
authors.forename: C
authors.initials: C
authors.lastname: Yin
authors.forename: S
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D000880:Anthraquinones; D001158:Arteries / Q000293:injuries*; D015153:Blotting, Western; D002478:Cells, Cultured; D004642:Emodin / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000880:Anthraquinones; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; C518327:aloe emodin; D004642:Emodin