title: Genomic diversity of group A rotavirus strains infecting humans in eastern India.
abstract: Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and multiplex PCR. The predominant genotype was G1P[8] (20%), followed by G2P[4] (15%) and G4P[8] (6%). A number of uncommon genotypes, G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4] (2.5%), and G4P[6] (1.25%), were also detected during this study period. Twenty two percent of specimens showed mixed infections, 38 (24%) of the total samples remained untypeable for either VP7 or VP4, while only 4 (2.5%) of the samples were untypeable for both genes. Eleven specimens collected from Manipur were also genotyped and revealed a very high degree of genomic reassortment.
authors.affiliation: Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors.forename: Soma
authors.initials: S
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Sen
authors.forename: G
authors.initials: G
authors.lastname: Uma
authors.forename: V
authors.initials: V
authors.lastname: Varghese
authors.forename: S
authors.initials: S
authors.lastname: Chaudhuri
authors.forename: S K
authors.initials: SK
authors.lastname: Bhattacharya
authors.forename: T
authors.initials: T
authors.lastname: Krishnan
authors.forename: P
authors.initials: P
authors.lastname: Dutta
authors.forename: D
authors.initials: D
authors.lastname: Dutta
authors.forename: M K
authors.initials: MK
authors.lastname: Bhattacharya
authors.forename: U
authors.initials: U
authors.lastname: Mitra
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: T N
authors.initials: TN
authors.lastname: Naik
mesh_terms: D002675:Child, Preschool; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012703:Serotyping