Effect of deuterium oxide on actomyosin motility in vitro.
Actin filament velocities in an in vitro motility assay system were measured both in heavy water (deuterium oxide, D(2)O) and water (H(2)O) to examine the effect of D(2)O on the actomyosin interaction. The dependence of the sliding velocity on pD of the D(2)O assay solution showed a broad pD optimum of around pD 8.5 which resembled the broad pH optimum (pH 8.5) of the H(2)O assay solution, but the maximum velocity (4.1+/-0.5 microm/s, n=11) at pD 8.5 in D(2)O was about 60% of that (7.1+/-1.1 microm/s, n=11) at pH 8.5 in H(2)O. The K(m) values of 95 and 80 microM and V(max) values of 3.2 and 5.1 microm/s for the D(2)O and H(2)O assay were obtained by fitting the ATP concentration dependence of the velocity (at pD and pH 7.5) to the Michaelis-Menten equation. The K(m) value of actin-activated Mg-ATPase activity of myosin subfragment 1 (S1) was decreased from 50 microM [actin] in H(2)O to 33 microM [actin] in D(2)O without any significant changes in V(max) (9.4 s(-1) in D(2)O and 9.3 s(-1) in H(2)O). The rate constants of ADP release from the acto-S1-ADP complex measured by the stopped flow method were 361+/-26 s(-1) (n=27) in D(2)O and 512+/-39 s(-1) (n=27) in H(2)O at 6 degrees C. These results suggest that the decrease in the in vitro actin-myosin sliding velocity in D(2)O results from a slowing of the release of ADP from the actomyosin-ADP complex and the increase in the affinity of actin for myosin in the presence of ATP in D(2)O.
Chaen
S
S
Department of Physiology, School of Medicine, Teikyo University, Tokyo 173-8605, Japan. schaen@rinshoken.or.jp
Yamamoto
N
N
Shirakawa
I
I
Sugi
H
H
D008841:Actin Cytoskeleton / Q000737:chemistry; D000205:Actomyosin / Q000737:chemistry*; D000244:Adenosine Diphosphate / Q000737:chemistry; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000737:chemistry; D017666:Deuterium Oxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009218:Myosins / Q000737:chemistry; D011817:Rabbits; D012212:Rheology; D014867:Water / Q000737:chemistry
D014867:Water; D000244:Adenosine Diphosphate; D000205:Actomyosin; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins; D017666:Deuterium Oxide