Isolation of Neospora caninum from the brain of a pregnant sheep.
Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction.
Koyama
T
T
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Kobayashi
Y
Y
Omata
Y
Y
Yamada
M
M
Furuoka
H
H
Maeda
R
R
Matsui
T
T
Saito
A
A
Mikami
T
T
D000818:Animals; D001921:Brain / Q000469:parasitology*; D003048:Coccidiosis / Q000151:congenital / Q000662:veterinary*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000302:isolation & purification* / Q000648:ultrastructure; D010179:Pancreas / Q000469:parasitology / Q000648:ultrastructure; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*