[Retroviral transduction of a mutant erbB-2 gene into human CD34+ derived dendritic cells].
OBJECTIVE
To successfully transduce a mutant erbB-2 gene into normal human CD34(+)-derived dendritic cells (DCs) and verify gene expression in these transduced dendritic cells.


METHODS
The packaging cell line PA317 was transfected with mutant erbB-2 gene DNA and the virus produced was used to infect packaging cell line PG13. The virus produced by PG13 was used to infect CD34(+)-derived dendritic cells by the spinoculation method using flasks coated with fibronectin material to facilitate retrovirus gene transter efficiency and the mutant erbB-2 gene expression was assessed by ABC staining and FACScan methods.


RESULTS
A mutant erbB-2 gene packaging cell line was produced and this mutant gene was transduced into human CD34(+)-derived DCs. It was verified that the relatively large numbers of the transduced DCs expressed the mutant erbB-2 protein which was eradicated of the ability to transform mouse NIH3T3 fibroblast cells.


CONCLUSIONS
Human DCs can be gene-modified and these gene-modified DCs may be useful in stimulating T lymphocytes for immunotherapy.
He
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Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078, China.
Hong
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D016475:3T3 Cells; D000818:Animals; D001943:Breast Neoplasms / Q000628:therapy; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D022761:Gene Transfer, Horizontal; D018734:Genes, erbB-2; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D009154:Mutation; D012190:Retroviridae / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured