In-vitro cultivation of normal human oral keratinocytes.
OBJECTIVE
To establish a method for culturing normal human oral keratinocytes.


METHODS
Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.


RESULTS
Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody.


CONCLUSIONS
Human oral keratinocytes have been successfully grown in serial culture.
Zhou
Z
Z
Department of Oral Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Zhou
H
H
Shang
Q
Q
Cao
Y
Y
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D007633:Keratins / Q000032:analysis; D009061:Mouth Mucosa / Q000166:cytology*
D007633:Keratins