AP180 binds to the C-terminal SH2 domain of phospholipase C-gamma1 and inhibits its enzymatic activity.
The role of phospholipase Cgamma1 (PLCgamma1) in signal transduction was investigated by characterizing its SH domain-binding proteins that may represent components of a novel signaling pathway. A 180-kDa protein that binds to the SH2 domain of PLCgamma1 was purified from rat brain. The amino acid sequence of peptide derived from the purified protein is now identified as AP180, a clathrin assembly protein that has been implicated in clathrin-mediated synaptic vesicle recycling in synapses. In this report, we demonstrate the stable association of PLCgamma1 with AP180 in a clathrin-coated vesicle complex, which not only binds to the carboxyl-terminal SH2 domain of PLCgamma1, but also inhibits its enzymatic activity in a dose-dependent manner.
Han
Seung Jin
SJ
School of Biological Sciences, Seoul National University, Seoul, Korea.
Lee
Jung Hyun
JH
Hong
Seung Hwan
SH
Park
Sang Dai
SD
Kim
Chul Geun
CG
Song
Min Dong
MD
Park
Tae Kyu
TK
Kim
Chan Gil
CG
D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002966:Clathrin / Q000378:metabolism; D004705:Endocytosis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism; D015151:Immunoblotting; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D034041:Monomeric Clathrin Assembly Proteins; D010455:Peptides / Q000737:chemistry; D051966:Phospholipase C gamma; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013347:Subcellular Fractions; D013997:Time Factors; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D018909:src Homology Domains
D033942:Adaptor Proteins, Vesicular Transport; D002352:Carrier Proteins; D002966:Clathrin; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D008565:Membrane Proteins; D034041:Monomeric Clathrin Assembly Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C439023:clathrin assembly protein AP180; D005982:Glutathione Transferase; D010738:Type C Phospholipases; D051966:Phospholipase C gamma