title: Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis.
authors.affiliation: Department of Structural Chemistry, Schering-Plough Research Institute, Kenilworth, NJ, USA.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Wang
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Malcolm
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000138:chemical synthesis; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents