IL-2-driven natural killer cell generation: role of anti-H-2b monoclonal antibodies and stromal cells in controlling quantitative and repertoire changes.
To investigate the role of major histocompatibility complex class I and bone marrow stromal cells on in vitro differentiation of natural killer cells, a CD44(low/-) CD2- population was isolated from mouse bone marrow. This NK-1.1- CD3- LFA-3+ B220+ population, when stimulated with IL-2 and co-cultured with supportive syngeneic stromal cells, generated populations of NK-1.1+ Ly49A+ Ly49C/I+ CD3- mature natural killer cells. The effect of anti-H-2b monoclonal antibodies (mAbs) on this phenomenon was assayed. Pre-adhesion of anti-H-2b mAbs to the stromal cells did not exert any effect, whereas when the same mAbs were pre-adhered to progenitors, there was a inhibition of natural killer cell generation that was maximum when the mAbs were added directly to cultures. In addition, the anti-H-2b mAbs did not inhibit the IL-2-induced proliferation of mature natural killer cells. Allogeneic but not H-2b-deficient stromal cells decreased the expression of Ly-49C/I but not Ly49A, thus suggesting that stromal cell haplotypes qualitatively influence the expression of Ly49s repertoire.
Agostini
M
M
Department of Clinical and Experimental Medicine, University of Perugia, Italy.
Di Marco
B
B
Spinicelli
S
S
Bruscoli
S
S
Delfino
D V
DV
D000818:Animals; D000950:Antigens, Ly; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002455:Cell Division; D005786:Gene Expression Regulation; D006183:H-2 Antigens / Q000276:immunology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000502:physiology*; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D055650:NK Cell Lectin-Like Receptor Subfamily A; D055630:Receptors, NK Cell Lectin-Like; D017154:Stromal Cells / Q000276:immunology
D000950:Antigens, Ly; D000970:Antineoplastic Agents; D006183:H-2 Antigens; D007376:Interleukin-2; C527088:Klra1 protein, mouse; C527091:Klra3 protein, mouse; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D055650:NK Cell Lectin-Like Receptor Subfamily A; D055630:Receptors, NK Cell Lectin-Like