Comparative DNA sequence analysis of genetic variation in the African grey parrot, Psittacus erythacus.
Comparative genome analysis promises to provide an insight into avian species that have been very little studied. To test the feasibility of this approach, we investigated the use of heterologous primers to generate single nucleotide polymorphisms (SNP) in the African grey parrot, Psittacus erythacus, using primers specific for chicken and turkey DNA fragments. Three of the primers were specific for three expressed sequence tagged sites in the turkey and the fourth for a chicken proteoglycan core protein-like DNA sequence. A total of about 2200 bp of the parrot genome was evaluated for DNA sequence variation. Seven SNPs were identified and confirmed by Mendelian segregation. The frequency distribution of the most common nucleotide at each SNP locus in an unrelated group of parrots ranged from 0.84 to 0.97. The percent similarity of each parrot sequence to the reference sequence was inconsistent and ranged from zero to 100%. The primers as well as the nucleotide variants described represent valuable resources for genetic analysis in the parrot.
Shi
L
L
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg 24061, USA.
Drummond
P
P
De Kloet
S
S
Pimentel-Smith
G E
GE
Smith
E J
EJ
D000818:Animals; D002645:Chickens / Q000235:genetics; D014644:Genetic Variation; D010311:Parrots / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D014422:Turkeys / Q000235:genetics