[Study on apoptosis and its related genes in pregnancy induced hypertension].
OBJECTIVE
To investigate apoptosis and its related genes expression in serious pregnancy induced hypertension (SPIH).


METHODS
40 cases of normal term pregnancy(NTP) and 40 cases of SPIH were enrolled in this study. Apoptosis was detected using DNA in situ terminal deoxynucleotidyl transferas (TDT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick ending labeling (TUNEL); bax and bcl-2 were detected by immunohistochemical staining (SP).


RESULTS
In NTP group apoptosis index(AI) of cytotrophoblasts(CT) and syncytiophoblasts(ST) in placenta was (1.1 +/- 0.9)% and (41.8 +/- 1.5)%; bax positive rate was (1.0 +/- 0.9)% and (28.9 +/- 9.7)%; bcl-2 was (2.2 +/- 0.8)% and (22.9 +/- 0.7)% respectively; bax/bcl-2 was 0.7-1.7. In SPIH group, AI was (4.3 +/- 1.2)% and (45.3% +/- 1.4)%; bax was (2.2 +/- 0.8)% and (42.5 +/- 11.7)%; bcl-2 was (3.2 +/- 0.8)% and (23.3 +/- 7.8)%; bax/bcl-2 was 1.0-3.2. The difference between SPIH and NTP was significant respectively (P < 0.01); bax/bcl-2 was balance in NTP, while the balance was broken in SPIH.


CONCLUSIONS
Apoptosis and its related genes expression are simultaneous. The balance and imbalance of apoptosis and bax/bcl-2 affect the structure and the function of placenta, and then may further affect the outcome of pregnancy.
Li
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C
Department of Obstetrics and Gynecology, First Affiliated Hospital, Hubei Medical University, Wuhan 430060, Chian.
Hu
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Z
Cao
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L
D000328:Adult; D017209:Apoptosis; D005260:Female; D006801:Humans; D006973:Hypertension / Q000473:pathology*; D010920:Placenta / Q000737:chemistry / Q000473:pathology*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein