BIA-MS-MS: biomolecular interaction analysis for functional proteomics.
One of the experimental processes of functional proteomics is the analysis of protein interaction. Here, we review a new analytical platform, BIA-MS, for protein interaction analysis. BIA-MS is an integration of a surface plasmon resonance biosensor for real-time interaction analysis and mass spectrometry for the subsequent identification of interacting molecules.
Natsume
T
T
National Institute of Advanced Science and Technology, Biological Information Research Center (JBIRC), Tokyo, Japan. natsume@jbirc.aist.go.jp
Nakayama
H
H
Isobe
T
T
D015374:Biosensing Techniques; D013058:Mass Spectrometry / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D020539:Sequence Analysis, Protein / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques
D011506:Proteins