Effect of four acrylic bone cements on transforming growth factor-beta1 expression by osteoblast-like cells MG63.
Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-beta1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, following 1 h- and 7 day-curing. MG63 cells seldom expressed mRNA specific for TGF-beta1 in basal conditions. The cultures expressed mRNA constantly after incubation with the extract of CMW 1 cured for 1 h. TGF-beta1 specific mRNA was seldom expressed after incubation with the other cement extracts. The release of TGF-beta1 into the conditioned medium was increased significantly by CMW 1 extract at 1 h-curing, but was not changed significantly by CMW 1 extract at 7 day-curing and by the extracts of the other cements, at both curing times. The stimulating effect of CMW 1 on the secretion of TGF-beta1, even with all the restrictions of an in vitro study of continuous cell lines, if confirmed in vivo, might favor the development of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis.
Cenni
Elisabetta
E
Dipartimento Putti, Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
Granchi
Donatella
D
Ciapetti
Gabriela
G
Savarino
Lucia
L
Corradini
Alessandra
A
D000179:Acrylates; D001483:Base Sequence; D001843:Bone Cements; D002460:Cell Line; D017077:Culture Media, Conditioned; D017931:DNA Primers; D018076:DNA, Complementary; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics*
D000179:Acrylates; D001843:Bone Cements; D017077:Culture Media, Conditioned; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta