title: Evidence of a new biotransformation pathway of p-coumaric acid into p-hydroxybenzaldehyde in Pycnoporus cinnabarinus.
abstract: Pycnoporus cinnabarinus MUCL39533 was shown to be able to convert p-coumaric acid into p-hydroxybenzaldehyde, a component of high organoleptic note present in natural vanilla aroma. Use of phospholipid-enriched medium led to high-density cultures of P. cinnabarinus, since dry mycelial biomass was increased three-fold as compared to glucose medium. In the presence of phospholipids, 155 mg l(-1) p-hydroxybenzaldehyde was produced as the major compound on culture day 13 with a molar yield of 26%. The degradation pathways of p-coumaric acid were investigated. Based on the different metabolites identified, an oxidative side-chain degradation pathway of p-coumaric acid conversion to p-hydroxybenzoic acid was suggested. This acid was further reduced to p-hydroxybenzaldehyde and p-hydroxybenzyl alcohol, or hydroxylated and reduced to protocatechyl derivatives. Additionally, a reductive pathway of p-coumaric acid with 3-(4-hydroxyphenyl)-propanol as the terminal product occurred.
authors.affiliation: Unité INRA de Biotechnologie des Champignons Filamenteux, IFR de Biotechnologie Agro-Industrielle de Marseille, Universités de Provence et de la Méditerranée, ESIL, France. estrada@esil.univ-mrs.fr
authors.forename: I
authors.initials: I
authors.lastname: Estrada Alvarado
authors.forename: A
authors.initials: A
authors.lastname: Lomascolo
authors.forename: D
authors.initials: D
authors.lastname: Navarro
authors.forename: M
authors.initials: M
authors.lastname: Delattre
authors.forename: M
authors.initials: M
authors.lastname: Asther
authors.forename: L
authors.initials: L
authors.lastname: Lesage-Meessen
mesh_terms: D000363:Agaricales / Q000254:growth & development / Q000378:metabolism*; D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism*; D001709:Biotechnology; D001711:Biotransformation; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D005421:Flavoring Agents / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D011422:Propionates
chemical_list: D001547:Benzaldehydes; D003373:Coumaric Acids; D005421:Flavoring Agents; D010743:Phospholipids; D011422:Propionates; C495469:p-coumaric acid; C011483:4-hydroxybenzaldehyde