[Investigation of the clinicopathologic features, immunophenotype and Epstein-Barr virus infection of primary central nervous system lymphoma].
OBJECTIVE
To investigate the clinicopathological features, immunophenotypes and EB virus infection of 25 cases of primary central nervous system lymphoma (PCNSL).


METHODS
Immunohistochemical staining was performed using monoclonal antibodies, L26 (CD20), kappa,lambda for B lymphocytes, UCHL-1 (CD45RO) for T lymphocytes, CS1-4 for latent membrane protein-1 (LMP-1) and GFAP for gliocytes. In situ hybridization was used for detection of EB virus encoded small nuclear RNAs (EBER1/2).


RESULTS
All of the 25 PCNSL were of B lymphocyte origin with high-grade malignancy. Only 2/25 (8%) cases showed positive signal in EBER1/2 in situ hybridization.


CONCLUSIONS
This group of PCNSL were all B-cell lymphomas, and the role of EB virus in this group of PCNSL was uncertain.
Xu
H
H
Department of Pathology, First University Hospital, West-China University of Medical Sciences, Chengdu 610041, China. xu141@sohu.com
Li
G
G
Liu
W
W
Zhang
S
S
Li
F
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D016543:Central Nervous System Neoplasms / Q000150:complications / Q000276:immunology / Q000473:pathology / Q000821:virology*; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000276:immunology; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged