Immunocytochemical observations on the organ distribution of exogenous monomeric and dimerized lysozyme.
Using commercial anti-lysozyme antibodies and anti-dimerized lysozyme rabbit serum produced by us we demonstrated by immunohistochemistry, in some organs of rats, the expression of exogenous egg white lysozyme preparations beside the native lysozyme. After oral administration, the egg white lysozyme was detected in intestinal epithelium, proximal and distal tubules of some nephrons, pulmonary alveolar walls and hepatocytes in the 3rd zone of liver acini, whereas native lysozyme was strongly expressed in intestinal and pulmonary macrophages in both the experimental and control animals. However, expression of the dimerized lysozyme released from the intraperitoneally implanted mini-osmotic pumps and detected using specific antisera was evident only on erythrocytes in intestinal blood vessels. It is concluded that the lysozyme preparations administered per os or parenterally are resorbed to blood circulation and distributed among various organs in an active form and maintaining their antigenic specificity. It may speak for their direct anti-inflammatory and immunomodulatory effects in respiratory, urinary, digestive and other systems.
Lukaszyk
A
A
Department of Histology and Embryology, University of Medical Sciences, Poznań, Poland. anluk@mail.usoms.poznan.pl
Rafińska
L
L
Jaroszewski
J
J
Klein
P
P
Stolzmann
W
W
Pieścikowska
I
I
Sawiński
P
P
D000818:Animals; D019281:Dimerization; D007150:Immunohistochemistry; D007583:Jejunum / Q000166:cytology / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry* / Q000276:immunology / Q000493:pharmacokinetics*; D009995:Osmosis; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D009113:Muramidase