title: Detection of Chlamydia pneumonia DNA in nasopharyngolaryngeal swab samples from patients with rhinitis and pharyngolaryngitis with polymerase chain reaction.
abstract: OBJECTIVE
  To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease.
  
  
  METHODS
  PCR assay was used to detect Chlamydia pneumonia specific Pst I 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis. C. pneumonia specific antibodies in sera were also assayed with microimmuno-fluoresence (MIF).
  
  
  RESULTS
  About 28% (49/175) of the patients were PCR positive and 25.7% (45/175) were MIF antibodies positive. The accordance rate of the two methods was 91.8%.
  
  
  CONCLUSIONS
  It is suggested that the C. pneumonia infection was common in this group of patients and the C. pneumonia Pst I 474 specific PCR was sensitive and specific for detecting C. pneumonia in pharyngolaryngitis or rhinitis and sinusitis.
authors.affiliation: Department of Otolaryngology, Third Clinical College, Sun Yat-sen University of Medical Sciences, Guangzhou 510630, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Ning
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D023521:Chlamydophila Infections / Q000382:microbiology / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans; D007827:Laryngitis / Q000382:microbiology*; D010612:Pharyngitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012220:Rhinitis / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial