[Existence of IL-8 degrading protease and anti-IL-8 IgG in gingival crevicular fluid].
OBJECTIVE
To identify the nature of the IL-8 inhibitor(s) in gingival crevicul fluid (GCF).


METHODS
(1) 14 GCF samples were collected from 13 adult periodontitis (AP) patients, and 9 samples were taken from 8 healthy subjects. Each GCF sample was divided into two aliquots. A serine protease-specific inhibitor-PMSF was added to one aliquot and PBS added to the other aliquot as control. ELISA was used to measure IL-8 level in the samples. (2) 41 GCF samples were collected from 15 AP patients and indirect ELISA was performed to detect the anti-IL-8 IgG antibody.


RESULTS
(1) IL-8 level in the PMSF-added samples were significantly greater than that in the control group(3.01 mg/L +/- 5.79 mg/L vs 0.05 mg/L +/- 0.15 mg/L, P < 0.001). (2) The mean value of anti-IL-8 IgG in GCF was greater than that of negative control +3 x s.


CONCLUSIONS
A serine protease which can "cleave" IL-8 exists in GCF and GCF from AP sites contain auto-antibody against IL-8.
Cao
C
C
School of Stomatology, Beijing Medical University, Beijing 100081.
Xie
H
H
Ma
D
D
D001323:Autoantibodies / Q000032:analysis*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D008297:Male; D012697:Serine Endopeptidases / Q000032:analysis*
D001323:Autoantibodies; D016209:Interleukin-8; D012697:Serine Endopeptidases