A new antifungal protein and a chitinase with prominent macrophage-stimulating activity from seeds of Phaseolus vulgaris cv. pinto.
From the seeds of the pinto bean (Phaseolus vulgaris cv. pinto), a chitinase and a novel antifungal protein, both with the ability of markedly augmenting nitrite production by murine peritoneal macrophages, were isolated. The antifungal proteins, designated phasein A and phasein B, exhibited molecular weights of 28 and 32 kDa, respectively. Phaseins A and B were adsorbed on Affi-gel blue gel and CM-Sepharose and were eluted as adjacent peaks from CM-Sepharose. Phasein A demonstrated potent antifungal activity toward Fusarium oxysporum and Physalospora piricola. Phasein B was more potent than phasein A toward P. piricola but less potent than phasein A toward F. oxysporum and Rhizoctonia solani. Both antifungal proteins inhibited the activity of HIV-1 reverse transcriptase and translation in a rabbit reticulocyte lysate system, with phasein B being more potent. Nitrite production by mouse macrophages was greatly boosted in the presence of both phaseins A and B, although the effect of phasein A was more prominent. The bioactivities of phaseins were in general potent compared with those of other antifungal proteins.
Ye
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Department of Biochemistry, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
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D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002474:Cell-Free System / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation / Q000187:drug effects; D005658:Fungi / Q000187:drug effects; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D008262:Macrophage Activation / Q000187:drug effects*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D027805:Phaseolus; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014176:Protein Biosynthesis / Q000187:drug effects; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D000935:Antifungal Agents; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D002688:Chitinases; C446298:phasein A; C446299:phasein B