title: [A preliminary study on translocation of the immunoglobulin heavy chain gene in KM3 myeloma cell line].
abstract: OBJECTIVE
  To identify the IgH translocation in KM3 myeloma cell line established from a Chinese patients.
  
  
  METHODS
  Southern blotting was performed with dig-labeled probes specific for IgH switch regions to identify and distinguish IgH switch recombination events.
  
  
  RESULTS
  Besides a 10.0 kb Hind III fragment being able to hybridize with both 5' S mu and 3' S mu probes as that in placenta genome DNA. There was a 5.6 kb Hind III fragment in KM3 genome DNA could only hybridize with the 3' S mu probe.
  
  
  CONCLUSIONS
  There was an illegitimate switch recombination fragment containing 3' S mu region in the KM3 myeloma cell line, which was caused by the IgH translocation involving the IgH S mu region.
authors.affiliation: Department of Hematology, Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: H
authors.initials: H
authors.lastname: Shen
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.forename: H
authors.initials: H
authors.lastname: Liu
mesh_terms: D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D007143:Immunoglobulin Heavy Chains; D004247:DNA; D015247:Deoxyribonuclease HindIII