Retinoic acid regulated insulin-like growth factor gene expression in cord blood lymphocytes.
OBJECTIVE
To explore the immune promotion mechanism of retinoic acid (RA).


METHODS
Reverse Transcription-Polymerase chain reaction (RT-PCR) was used to detect the gene expression of insulin like growth factor I (IGF-I) and IGF receptors (IGF-IR and IGF-IIR) in vitro, and their regulation by RA in human cord blood lymphocyte (CBL).


RESULTS
Significant upregulation of IGF-I, IGF-IR and IGF-IIR mRNA was found at 6-24 hours in CBL incubated with physiologic concentrations of RA as compared to those without RA.


CONCLUSIONS
The enhancement of IGF expression may be an important pathway for vitamin A to promote immune cellular functions.
Yang
Y
Y
Pediatrics Hospital, Shanghai Medical University, Shanghai 200032, China. yyang@shmu.edu.cn
Wang
W
W
Su
Y
Y
D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics; D017527:Receptor, IGF Type 2 / Q000235:genetics; D013002:Somatomedins / Q000235:genetics*; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D013002:Somatomedins; D014212:Tretinoin; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1