[Characteristics of Triticale glutaminase].
The glutaminase (EC 3.5.1.2) isolated from seedlings of triticale (Triticale sp.) had a pH optimum of about 8, was inhibited with excess substrate (glutamine), and reaction products (glutamate and NH4+). A monovalent anion (Cl-) and a multivalent anion (phosphate) were shown to activate the glutaminase. Some features of the glutaminase from triticale were similar to those of animal glutaminase activated by phosphate and were different from features of the enzyme from Escherichia coli.
Sidel'nikova
L I
LI
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.
Evstigneeva
Z G
ZG
Solov'eva
N A
NA
Shatilov
V R
VR
D000641:Ammonia / Q000737:chemistry; D000838:Anions; D002523:Edible Grain / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D005972:Glutaminase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D005973:Glutamine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012639:Seeds / Q000737:chemistry
D000838:Anions; D004791:Enzyme Inhibitors; D005973:Glutamine; D018698:Glutamic Acid; D000641:Ammonia; D005972:Glutaminase
Nekotorye svoĭstva glutaminazy Tritikale.