[Functional status of p53 and chemosensitivity of colon cancer cell lines].
OBJECTIVE
To study the functional status of tumor suppressor gene p53 in relation to chemotherapeutic sensitivity in colon cancer cell lines.


METHODS
With the use of functional assay (FASAY) of p53, the existing p53 status (wt, mt) of 6 colon cancer cell lines was determined. The difference in chemotherapeutic sensitivity of colon cancer cells with wild-type p53 was compared with those with mutated type p53 by MTT method, followed by cytometic analysis of cell cycle.


RESULTS
The chemotherapeutic sensitivity of Lovo and LS 174T cancer cell lines with wild-type p53 was 5-10 times higher than that of 4 other cell lines with mutated p53. Selective apoptosis and cell cycle arrest in G1 phase occurred when treated with adriamycin.


CONCLUSIONS
The functional status of p53 in human colon cancer cell lines directly affects their sensitivity to chemotherapeutic agents.
Jia
L
L
Department of Oncology, China-Japan Friendship Hospital, Beijing 100029, China.
Yu
L
L
Zhu
S
S
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D003110:Colonic Neoplasms; D004317:Doxorubicin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin