Effect of L929 cell-conditioned medium on antioxidant capacity in RAW264.7 cells.
Previously, we found that macrophage colony-stimulating factor (M-CSF) could reduce tert-butyl hydroperoxide (tbOOH)-induced oxidative injury in monocytes/macrophages. In order to find the mechanism by which M-CSF achieves this, we investigate the effect of M-CSF on the antioxidant system in murine macrophage RAW264.7 cells, using L929 cell-conditioned medium (L929-CM) as the source of M-CSF. The results show that L929-CM increased selenium-dependent glutathione peroxidase, non-selenium-dependent glutathione peroxidase, and catalase activities; and it increased reduced glutathione (GSH) but decreased lipoperoxide content in RAW264.7 cells. L929-CM-treated cells maintained their antioxidant capacity (including antioxidant enzyme activities and GSH content) when challenged with tbOOH. We conclude that M-CSF from L929-CM reduces oxidative injury in RAW264.7 cells by increasing antioxidant enzyme activity and improving redox status.
Pang
Z J
ZJ
Department of Obstetrics and Gynaecology, Nanfang Hospital, Guangzhou, People's Republic of China. pangzhan@263.net
Chen
Y
Y
Xing
F Q
FQ
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants; D017077:Culture Media, Conditioned; D016173:Macrophage Colony-Stimulating Factor