Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis.
A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitive, inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as such was a suitable template for the detection of C. parvum by various PCR amplification targets.
Millar
C
C
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom.
Moore
J
J
Lowery
C
C
McCorry
K
K
Dooley
J
J
D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan / Q000032:analysis* / Q000235:genetics; D004196:Disease Outbreaks; D005243:Feces / Q000382:microbiology; D005784:Gene Amplification; D006801:Humans; D009865:Oocytes / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan