[Effects of human interleukin-2 gene transfer on the immunity of ovarian cancer cell line SKOV3].
OBJECTIVE
To study the effect of interleukin-2 (IL-2) gene transfer on the immunity of ovarian cancer cell line SKOV3.


METHODS
The expression of main histologic complex (MHC) molecular HLA-ABC, HLA-DR and HLA-DQ of SKOV3, SKOV3/Neo and SKOV3/IL-2 cells were detected by flow cytometry. The 51Cr-4 hr releasing test was employed to detect the killing effects of natural killer (NK) and lymphokine-activated killer (LAK) cells. Meanwhile, the tumor cell and lymphocyte co-culture was performed.


RESULTS
The expression of HLA-ABC, HLA-DR and HLA-DQ molecular of hIL-2 gene modified cells was upregulated markedly. The 51Cr-4 hr releasing test showed that the parental and gene modified cells were markedly resistant to NK cells, but the IL-2 gene modified cells was more sensitive to LAK cells. There were significant differences between them (P < 0.01). The "cross-talk" of tumor cell with lymphocyte was observed when the hIL-2 gene modified SKOV3 cells were cocultured with lymphocyte.


CONCLUSIONS
IL-2 gene transfer can enhance the immunity of ovarian cancer cell line SKOV3.
Zhang
Z
Z
Deptment of Obstetrics and Gynecology, General Hospital of Beijing Military Command, Beijing 100700, China.
Lang
J
J
D003602:Cytotoxicity, Immunologic; D005260:Female; D022761:Gene Transfer, Horizontal; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D010051:Ovarian Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D006649:Histocompatibility Antigens; D007376:Interleukin-2