Structural and serological relatedness of the O-antigens of Proteus penneri 1 and 4 from a novel Proteus serogroup O72.
O-specific polysaccharides (O-antigens) of the lipopolysaccharides (LPS) of Proteus penneri strains 1 and 4 were studied using sugar analysis, (1)H and (13)C NMR spectroscopy, including 2D COSY, H-detected (1)H,(13)C HMQC, and rotating-frame NOE spectroscopy (ROESY). The following structures of the tetrasaccharide (strain 1) and pentasaccharide (strain 4) repeating units of the polysaccharides were established: [reaction: see text]. In the polysaccharide of P. penneri strain 4, glycosylation with the lateral Glc residue (75%) and O-acetylation of the lateral GalNAc residue (55%) are nonstoichiometric. This polysaccharide contains also other, minor O-acetyl groups, whose positions were not determined. The structural similarity of the O-specific polysaccharides was consistent with the close serological relatedness of the LPS, which was demonstrated by immunochemical studies with O-antisera against P. penneri 1 and 4. Based on these data, it was proposed to classify P. penneri strains 1 and 4 into a new Proteus serogroup, O72, as two subgroups, O72a and O72a,b, respectively. Serological cross-reactivity of P. penneri 1 O-antiserum with the LPS of P. penneri 40 and 41 was substantiated by the presence of an epitope(s) on the LPS core region shared by all P. penneri strains studied.
Sidorczyk
Zygmunt
Z
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łodź, Poland. zsidor@biol.uni.lodz.pl
Toukach
Filip V
FV
Zych
Krystyna
K
Drzewiecka
Dominika
D
Arbatsky
Nikolay P
NP
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
D000818:Animals; D007106:Immune Sera / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011511:Proteus / Q000145:classification* / Q000276:immunology; D011817:Rabbits; D012703:Serotyping
D007106:Immune Sera; D019081:O Antigens