title: [A comparative study on the sensitivity and specificity of cholinesterase and glutathione s-transferase in Gammarus pulex L].
abstract: Studies on the influences of lindane, pirimiphos methyl, permethrin, zinc and dodecyl linear alkybenzene sulfonate (LAS) on the activity and toxicity of cholinesterase (ChE) and glutathione s-transferase (GST) in Gammarus pulex L. showed that only pirimiphos methyl caused a change in ChE activity in Gammarus, with a significant reduction in enzyme activity after 24 h and 48 h exposure. Both lindane and permethrin caused a change in GST activity in Gammarus, with a significant increase in enzyme activity after 48 h exposure. Lindane alos caused a significant increase in GST activity after 24 h exposure. Biomarkers ChE and GST were demonstrated a high degree of specificity and sensitivity in comparison to the lethality assay, but GST activity was less specific than ChE activity.
authors.affiliation: State Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093. yindq@nju.edu.cn
authors.forename: D
authors.initials: D
authors.lastname: Yin
authors.forename: H
authors.initials: H
authors.lastname: Jin
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002802:Cholinesterases / Q000032:analysis* / Q000187:drug effects; D003445:Crustacea / Q000187:drug effects / Q000201:enzymology*; D004353:Drug Evaluation, Preclinical; D005982:Glutathione Transferase / Q000032:analysis* / Q000187:drug effects; D001556:Hexachlorocyclohexane / Q000494:pharmacology; D007306:Insecticides / Q000494:pharmacology; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D012680:Sensitivity and Specificity
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; C014153:pirimiphos methyl; D001556:Hexachlorocyclohexane; D005982:Glutathione Transferase; D002802:Cholinesterases