Drosophila JAB1/CSN5 acts in photoreceptor cells to induce glial cells.
Different classes of photoreceptor neurons (R cells) in the Drosophila compound eye form connections in different optic ganglia. The R1-R6 subclass connects to the first optic ganglion, the lamina, and relies upon glial cells as intermediate targets. Conversely, R cells promote glial cell development including migration of glial cells into the target region. Here, we show that the JAB1/CSN5 subunit of the COP9 signalosome complex is expressed in R cells, accumulates in the developing optic lobe neuropil, and through the analysis of a unique set of missense mutations, is required in R cells to induce lamina glial cell migration. In these CSN5 alleles, R1-R6 targeting is disrupted. Genetic analysis of protein null alleles further revealed that the COP9 signalosome is required at an earlier stage of development for R cell differentiation.
Suh
Greg S B
GS
Department of Biological Chemistry, Howard Hughes Medical Institute, The School of Medicine, University of California, Los Angeles, CA 90095, USA.
Poeck
Burkhard
B
Chouard
Tanguy
T
Oron
Efrat
E
Segal
Daniel
D
Chamovitz
Daniel A
DA
Zipursky
S Lawrence
SL
D000483:Alleles; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D000075686:COP9 Signalosome Complex; D002450:Cell Communication / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D002633:Chemotaxis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D004627:Embryonic Induction / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D005838:Genotype; D020439:Growth Cones / Q000378:metabolism / Q000648:ultrastructure; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D009030:Mosaicism / Q000235:genetics; D046912:Multiprotein Complexes; D020125:Mutation, Missense / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D019581:Neuropil / Q000166:cytology / Q000378:metabolism; D009899:Optic Lobe, Nonmammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D010447:Peptide Hydrolases; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics; D014795:Visual Pathways / Q000166:cytology / Q000196:embryology / Q000378:metabolism*
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D011506:Proteins; D014157:Transcription Factors; D010447:Peptide Hydrolases; C501491:CSN5 protein, Drosophila; D000075686:COP9 Signalosome Complex