Splitting and biopsy for bovine embryo sexing under field conditions.
Improvements on embryo micromanipulation techniques led to the use of embryo bisection technology in commercial embryo transfer programs, and made possible the direct genetic analysis of preimplantation bovine embryos by biopsy. For example, aspiration and microsection, allow bovine embryos sexing by detection of male-specific Y-chromosome in a sample of embryonic cells. We report on the application of the methodologies of splitting and biopsy of bovine embryos in field conditions, and on the results of embryo sex determination by the polymerase chain reaction (PCR). Pregnancy rates achieved with fresh bisected or biopsied embryos (50 to 60%) were similar to the fresh intact embryos (55 to 61%). The PCR protocol used for embryo sexing showed 92% to 94% of efficiency and 90 to 100% of accuracy. These results demonstrate these procedures are suitable for use in field conditions.
Lopes
R F
RF
Departamento de Ciências Morfológicas, ICBS, Porto Alegre-RS, CEP, Brasil. rfflopes@dna.cbiot.ufrgs.br
Forell
F
F
Oliveira
A T
AT
Rodrigues
J L
JL
D000818:Animals; D001707:Biopsy, Needle / Q000662:veterinary; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle / Q000196:embryology*; D015925:Cryopreservation / Q000662:veterinary; D005260:Female; D008297:Male; D008846:Micromanipulation / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011247:Pregnancy; D018873:Pregnancy Rate; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*