[Detection of TDH-producing Vibrio parahaemolyticus O3:K6 from naturally contaminated shellfish using an immunomagnetic separation method and chromogenic agar medium].
We attempted to isolate TDH-producing Vibrio parahaemolyticus O3:K6 from shellfish. Asari samples were incubated with TSB supplemented with 2% (w/v) NaCl for 6 h, and then the 6-h cultures were incubated with salt polymyxin broth for 18 h. After the two-step enrichment, a 1 ml portion of the culture was treated with magnetic beads coated with K6 antibody for immunoconcentration of V. parahaemolyticus O3:K6. The immunoconcentrated and untreated cultures were plated onto a chromogenic agar and TCBS agar media for isolation of V. parahaemolyticus. TDH-producing V. parahaemolyticus O3:K6 was isolated from 3 out of 66 lots (4.5%) of naturally contaminated Asari. Six of 4,265 colonies suspected as V. parahaemolyticus (0.14%) were TDH-producing V. parahaemolyticus O3:K6.
Hara-Kudo
Y
Y
Shizuoka Institute of Environment and Hygiene.
Sugiyama
K
K
Nishina
T
T
Saitoh
A
A
Nakagawa
H
H
Ichihara
T
T
Konuma
H
H
Hasegawa
J
J
Kumagai
S
S
D000362:Agar; D001427:Bacterial Toxins; D001431:Bacteriological Techniques / Q000379:methods; D002863:Chromogenic Compounds; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D018189:Immunomagnetic Separation; D012758:Shellfish / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification* / Q000378:metabolism
D001427:Bacterial Toxins; D002863:Chromogenic Compounds; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin; D000362:Agar