title: Storage media and temperature maintain normal anatomy of cadaveric human skin for transplantation to full-thickness skin wounds.
abstract: Cadaveric human skin provides an optimal temporary cover after early excision of full-thickness burns; however, engraftment is reduced greatly by cryopreservation. Refrigerated skin is generally preferred because of its rapid revascularization, presumably caused by its greater viability. In this study, the effects of storage solutions, temperature, and the changing of the storage media on skin graft anatomy were evaluated as an indicator of graft viability. Split-thickness human skin grafts (0.012-0.015 mm) were retrieved from cadaveric donors and grafted to circumferential, full-thickness skin wounds on athymic mice. After clinical determination of engraftment 3 months after grafting, 6-mm punch biopsy samples of the human skin were harvested and separated into two groups. Biopsy samples were stored in either saline or Eagle's minimal essential medium. Media were not changed or were changed every 3 days. All groups were stored at either 4 degrees C or room temperature (RT). After 5, 10, and 21 days of storage, biopsy samples were grafted onto athymic mice for 20 days. The biopsy grafts were then collected and prepared for histologic scoring on a scale of +4 (normal anatomy) to 0 (no epithelial cells). Significant differences in histologic scores were found by the nonparametric Kruskal-Wallis test followed by Wilcoxon pairwise comparison. Skin stored in media maintained better histologic anatomy than skin in saline, suggesting better maintenance of viability. There was also better preservation of anatomy after storage at RT for 21 days with media changes every 3 days when compared to unchanged media and all conditions at 4 degrees C. These results support the hypothesis that increased availability of nutrients and increased storage temperature maintain higher viability of cadaveric human skin for transplantation to full-thickness cutaneous wounds.
authors.affiliation: Ohio Valley Tissue and Skin Center and Shriners Burns Hospital, Cincinnati, USA.
authors.forename: E C
authors.initials: EC
authors.lastname: Robb
authors.forename: N
authors.initials: N
authors.lastname: Bechmann
authors.forename: R T
authors.initials: RT
authors.lastname: Plessinger
authors.forename: S T
authors.initials: ST
authors.lastname: Boyce
authors.forename: G D
authors.initials: GD
authors.lastname: Warden
authors.forename: R J
authors.initials: RJ
authors.lastname: Kagan
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology* / Q000601:surgery*; D002102:Cadaver; D003470:Culture Media; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D006085:Graft Survival; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D019188:Organ Preservation Solutions; D012867:Skin / Q000293:injuries* / Q000473:pathology*; D016038:Skin Transplantation / Q000473:pathology*; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D003470:Culture Media; D019188:Organ Preservation Solutions