Mass spectrometric investigations of human milk oligosaccharides.
Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.
Pfenninger
A
A
Instrumental Analytical Chemistry, University of Frankfurt/M., Germany.
Karas
M
M
Finke
B
B
Stahl
B
B
Sawatzki
G
G
D005591:Chemical Fractionation; D002850:Chromatography, Gel; D006801:Humans; D013058:Mass Spectrometry; D008895:Milk, Human / Q000737:chemistry*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides