title: Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis.
abstract: The cause(s) of sarcoidosis is unknown. Mycobacterium spp. are suspected in Europe and Propionibacterium spp. are suspected in Japan. The present international collaboration evaluated the possible etiological links between sarcoidosis and the suspected bacterial species. Formalin-fixed and paraffin-embedded sections of biopsy samples of lymph nodes, one from each of 108 patients with sarcoidosis and 65 patients with tuberculosis, together with 86 control samples, were collected from two institutes in Japan and three institutes in Italy, Germany, and England. Genomes of Propionibacterium acnes, Propionibacterium granulosum, Mycobacterium tuberculosis, Mycobacterium avium subsp. paratuberculosis, and Escherichia coli (as the control) were counted by quantitative real-time PCR. Either P. acnes or P. granulosum was found in all but two of the sarcoid samples. M. avium subsp. paratuberculosis was found in no sarcoid sample. M. tuberculosis was found in 0 to 9% of the sarcoid samples but in 65 to 100% of the tuberculosis samples. In sarcoid lymph nodes, the total numbers of genomes of P. acnes or P. granulosum were far more than those of M. tuberculosis. P. acnes or P. granulosum was found in 0 to 60% of the tuberculosis and control samples, but the total numbers of genomes of P. acnes or P. granulosum in such samples were less than those in sarcoid samples. Propionibacterium spp. are more likely than Mycobacteria spp. to be involved in the etiology of sarcoidosis, not only in Japanese but also in European patients with sarcoidosis.
authors.affiliation: Department of Human Pathology, School of Medicine, Tokyo Medical and Dental University, Japan. eishi.path@tmd.ac.jp
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Eishi
authors.forename: Moritaka
authors.initials: M
authors.lastname: Suga
authors.forename: Ikuo
authors.initials: I
authors.lastname: Ishige
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kobayashi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamada
authors.forename: Tamiko
authors.initials: T
authors.lastname: Takemura
authors.forename: Touichiro
authors.initials: T
authors.lastname: Takizawa
authors.forename: Morio
authors.initials: M
authors.lastname: Koike
authors.forename: Shoji
authors.initials: S
authors.lastname: Kudoh
authors.forename: Ulrich
authors.initials: U
authors.lastname: Costabel
authors.forename: Josune
authors.initials: J
authors.lastname: Guzman
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Rizzato
authors.forename: Marcello
authors.initials: M
authors.lastname: Gambacorta
authors.forename: Ronald
authors.initials: R
authors.lastname: du Bois
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Nicholson
authors.forename: Om P
authors.initials: OP
authors.lastname: Sharma
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ando
mesh_terms: D000193:Actinomycetales Infections / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D005060:Europe; D006801:Humans; D007564:Japan; D008198:Lymph Nodes / Q000382:microbiology*; D009161:Mycobacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011424:Propionibacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012507:Sarcoidosis / Q000382:microbiology*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
chemical_list: D004269:DNA, Bacterial; D019914:Taq Polymerase