title: Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process.
abstract: Application of ultraviolet light (UV-) irradiation to a photocrosslinkable chitosan (Az-CH-LA) aqueous solution resulted in an insoluble, flexible hydrogel like soft rubber within 60 s. The chitosan hydrogel could completely stop bleeding from a cut mouse tail within 30 s of UV-irradiation and could firmly adhere two pieces of sliced skins of mouse to each other. In order to evaluate its accelerating effect on wound healing, full thickness-skin incisions were made on the back of mice and subsequently an Az-CH-LA aqueous solution was added into the wound and irradiated with UV light for 90 s. Application of the chitosan hydrogel significantly induced wound contraction and accelerated wound closure and healing. Histological examinations also have demonstrated an advanced granulation tissue formation and epithelialization in the chitosan hydrogel treated wounds. The chitosan hydrogel due to its accelerating healing ability is considered to become an excellent dressing for wound occlusion and tissue adhesive in urgent hemostasis situations.
authors.affiliation: Research Institute, National Defense Medical College, Saitama, Japan. ishihara@res.ndmc.ac.jp
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Ono
authors.forename: Masato
authors.initials: M
authors.lastname: Sato
authors.forename: Makoto
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yura
authors.forename: Takemi
authors.initials: T
authors.lastname: Matsui
authors.forename: Hidemi
authors.initials: H
authors.lastname: Hattori
authors.forename: Maki
authors.initials: M
authors.lastname: Uenoyama
authors.forename: Akira
authors.initials: A
authors.lastname: Kurita
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001760:Bleeding Time; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D048271:Chitosan; D003432:Cross-Linking Reagents; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009779:Occlusive Dressings; D014466:Ultraviolet Rays; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D002686:Chitin; D048271:Chitosan