[Collagen-binding proteins of human oral spirochetes].
OBJECTIVE
To evaluate pathogenic effect of the collagen-binding proteins(CBP) of human oral spirochetes.


METHODS
Immunoblot analysis, immunoelectron microscopy and other experiments had been used.


RESULTS
It was showed that: type I, IV and V CBP were detected in heated and unheated preparations from Treponema denticola(Td) ATCC 33520 and Treponema socranskii (Ts) ATCC 35534. Few CBP, however, were detected in heated and unheated preparations from a recently characterized isloate, Treponema medium(Tm) strain G7201. 27,000 type V CBP of Td, 110,000 type I of Ts and 95,000 type IV of Tm were located on the envelopes of the individual cells. The adherence of Td to the collagen-coated surface was significantly greater than that of Tm.


CONCLUSIONS
The CBP on the oral spirochetal cells play an important role in their adherence to collagen-rich connective tissues of the host.
Li
M
M
Research Institute of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Liu
Z
Z
Umemoto
T
T
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D024062:Collagen Type V / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D009055:Mouth / Q000382:microbiology; D011485:Protein Binding; D038981:Receptors, Collagen; D013045:Species Specificity; D013144:Spirochaetales / Q000737:chemistry* / Q000502:physiology; D014208:Treponema / Q000737:chemistry / Q000502:physiology
D001426:Bacterial Proteins; D024042:Collagen Type I; D024141:Collagen Type IV; D024062:Collagen Type V; D016023:Integrins; D038981:Receptors, Collagen