[Influence of estrogen on the epithelial ovarian cancer cell lines in vitro].
OBJECTIVE
To study the effect of 17-beta estradiol on the growth of the epithelial ovarian carcinoma cell lines in vitro.


METHODS
The proliferative capacity of the ovarian carcinoma cell lines CAOV3 and OVCAR3 in the culture medium with 17-beta estradiol was evaluated by the means of the mircoculture tetrazolium assay (MTT) and cell kinetics was analyzed by flow cytometry (FCM). The expression of estrogen receptor (ER) of the two cell lines was examined by the immunohistochemical staining.


RESULTS
The growth of both two epithelial ovarian cancer cell lines was slightly inhibited by 17-beta estradiol (0.25-5.00 nmol/L), and the inhibitive rate was 6.3%-36.0%. FCM showed that the kinetics of the two cell lines were obviously changed. Comparing with the kinetics of CAOV3 without estradiol, the cell rate of Stage G0/G1 of CAOV3 with estradiol decreased from 55.0% to 19.0%-30.0%, cell rate of Stage S increased from 30.0% to 52.0%, cell rate of Stage G2/M increased from 14.0% to 17.0%-28.0%; In OVCAR3, cell rate of Stage C2/M decreased from 37% to 22.0%-30.0%, cell rate of Stage S increased from 31.0% to 38.0%-51.0%, cell rate of Stage C0/G1 did not changed much (cell rate without estradiol was 32.0%, while cell rate with estradiol 29.0%-31.0%). Estrogen receptors of the two cell lines were positive and not changed after cultured in the medium with 17-beta estradiol.


CONCLUSIONS
The proliferate capacity of the two epithelial ovarian cancer cell lines CAOV3 and OVCAR3 can be inhibited by the estrogen (0.25-5.00 nmol/L), possibly through the change of the tumor cell kinetics.
Yang
Y
Y
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Shen
K
K
Lang
J
J
D002453:Cell Cycle / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D011960:Receptors, Estrogen / Q000032:analysis; D014407:Tumor Cells, Cultured
D011960:Receptors, Estrogen; D004958:Estradiol