The heat shock response of Fusobacterium nucleatum.
The heat-shock response of the oral Gram-negative bacterium Fusobacterium nucleatum was examined. Different strains of F. nucleatum were grown at 37 C. 42 degrees C and 48 C in the presence of [35S]methionine. Cellular proteins synthesised after shifts to higher temperatures were analysed by SDS-PAGE and autoradiography. Strains ATCC 10953, F1, F3 and Fev1 exhibited heat-shock response, and major proteins were observed at 60, 70 and 90 kDa. but increased protein synthesis was also observed for other proteins. Immunoblot analysis, using a panel of antibodies directed to epitopes on different known heat-shock proteins revealed cross-reactive proteins, indicating homology between Escherichia coli, Mycobacterium leprae and F. nucleatum heat shock proteins.
Skar
C K
CK
Department of Odontology-Oral Microbiology, Dental Faculty, University of Bergen, Norway. cecilie.skar@odont.uib.no
Bakken
V
V
D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015151:Immunoblotting; D009166:Mycobacterium leprae / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli