[1,25(OH)2 vitamin D3 induces IL-1 alpha mRNA expression from osteoblastic-like and bone marrow co-culture cells].
OBJECTIVE
The purpose of this research is to investigate the relationship between 1,25(OH)2 Vitamin D3[1,25(OH)2D3] and Interleukin-1 (IL-1) in regulating osteoclast formation. The result may enrich our knowledge about biological mechanisms of periodontal tissue remodeling during orthodontic tooth movement.


METHODS
We found IL-1 alpha mRNA expression in bone marrow and osteoblastic cell co-cultures by semi-quantitative RT-PCR.


RESULTS
1,25(OH)2D3 increased IL-1 alpha mRNA expression from this co-culture cells in a dose dependent manner, and the expression reached its peak in day 2 and decreased thereafter.


CONCLUSIONS
These results demonstrate that IL-1 alpha involves in osteoclast formation induced by 1,25(OH)2D3. And we propose IL-1 alpha may stimulate the proliferation of osteoclast progenitor during the first 4 days of total 6 days culture period.
Zhang
D
D
School of Stomatology, Beijing Medical University, Beijing 100081.
Lorenzo
J
J
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology*; D018920:Coculture Techniques; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
D007375:Interleukin-1; D012333:RNA, Messenger; D002117:Calcitriol