Parthenogenetic activation and subsequent development of rat oocytes in vitro.
Studies were undertaken to determine whether electrical stimulation, or ethanol treatment alone or in combination with 6-dimethylaminopurine (6-DMAP) influenced the rate of parthenogenetic activation of rat oocytes. The percentages of activated oocytes with pronuclei (89-91%) and those developed to the two-cell stage (68-72%) were significantly higher after electrical stimulation with direct current (DC) at 100 V/mm, 99 microsec once or twice, than when other DC voltages (75, 150, and 200) were applied or when ethanol or 6-DMAP treatment was given alone. However, none of the activated oocytes developed beyond the four-cell stage. The percentages of activated oocytes with pronuclei (100%) that developed to the two-cell (100%), eight-cell (89%) and blastocyst stages (50%) were significantly higher when electrical stimulation was followed by treatment with 2 mM 6-DMAP for 4 hr than when other combined procedures were applied. In conclusion, the results of the present study clearly showed that combined treatment of electrical stimulation or ethanol with 6-DMAP induces parthenogenetic activation and subsequent development of rat oocytes in vitro.
Jiang
Jin-Yi
JY
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. jiang@bios.tohoku.ac.jp
Mizuno
Satoshi
S
Mizutani
Eiji
E
Sasada
Hiroshi
H
Sato
Eimei
E
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004558:Electric Stimulation; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development*; D010312:Parthenogenesis / Q000187:drug effects*; D051381:Rats
D000431:Ethanol; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine