Quantitative determination of aflatoxin B1-oxime by column liquid chromatography with ultraviolet detection.
Liquid chromatography was used for the quantification of aflatoxin B1-oxime (AFB1-oxime). The yield of AFB1-oxime in the reaction mixture was 89%, while after purification on silica gel it was 72%. LC analysis of the reaction mixture after silica gel fractionation revealed a retention time of 0.84 min for AFB1-oxime, 8.42 min for AFB1, 1.21 min for unknown 1 and 1.61 min for unknown 2. UV-visible analysis of the reaction mixture after silica gel fractionation showed a lambda(max) of 269 and 361 nm for AFB1-oxime, 263 and 360 nm for AFB1, 273 nm for unknown 1 and 275 nm for unknown 2. Excitation and emission wavelengths were found to be 269 and 368/438 nm for AFB1-oxime, 359/424 nm for AFB1, 270 and 367/450 nm for unknown 1 and 273 and 416/447 nm for unknown 2. The method may find versatile application in monitoring reactions for the preparation of oximes of various analytes for the synthesis of their immunogens.
Rastogi
S
S
Food Toxicology Division, Industrial Toxicology Research Centre, Mahatma Gandhi Marg, Post Box No. 80, Lucknow 226001, India.
Das
M
M
Khanna
S K
SK
D016604:Aflatoxin B1 / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D010091:Oximes / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D010091:Oximes; D016604:Aflatoxin B1