Transplantation of cultured human neural progenitor cells into rat brain: migration and differentiation.
We studied the fate in vitro cultured human stem/progenitor cells after transplantation into rat brain. The cells from human fetuses at 8-12 weeks' gestation were cultured in vitro for 14 days and transplanted into the brain of 10-day-old and adult rats. Microscopic examination showed that human stem/progenitor cells migrated into various regions of rat brain. Immunohistochemical assay demonstrated that some cells differentiated into astrocytes and neurons, while others retained the embryonic phenotype.
Aleksandrova
M A
MA
N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Poltavtseva
R A
RA
Marei
M V
MV
Revishchin
A V
AV
Saburina
I N
IN
Dubrovina
I V
IV
Korochkin
L I
LI
Sukhikh
G T
GT
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D016332:Fetal Tissue Transplantation; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors