title: Immune reactivity of human sera to the glycoprotein B of human herpesvirus 7.
abstract: The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7-seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.
authors.affiliation: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Franti
authors.forename: Jean-Thierry
authors.initials: JT
authors.lastname: Aubin
authors.forename: Guillemette
authors.initials: G
authors.lastname: De Saint-Maur
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kosuge
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
authors.forename: Agnes
authors.initials: A
authors.lastname: Gautheret-Dejean
authors.forename: Antoine
authors.initials: A
authors.lastname: Garbarg-Chenon
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Huraux
authors.forename: Henri
authors.initials: H
authors.lastname: Agut
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D016199:Herpesvirus 7, Human / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007223:Infant; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D019349:Roseolovirus Infections / Q000175:diagnosis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera; D010455:Peptides; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C105061:glycoprotein B, human herpesvirus 7