Simultaneous determination of delta-aminolevulinic acid, porphobilinogen, levulinic acid and glycine in culture broth by capillary electrophoresis.
Capillary electrophoretic simultaneous determination of a mixture containing delta-aminolevulinic acid, porphobilinogen, levulinic acid and glycine was investigated. With increases in the sodium tetraborate buffer concentration (5-70 mM), resolution of the four components was improved, but the migration time was increased. Alternatively, with increases in the applied voltage (5-22.5 kV), a shortened migration time was seen but this adversely affected resolution. The components were separated with high resolution by using a fused-silica capillary column (75 cm x 75 microm I.D.) filled with 30 mM sodium tetraborate buffer (pH 9.3-9.4) under the applied voltage of 20 kV (constant voltage mode). When the established method was applied to the culture broth of Rhodopseudomonas sphaeroides, a photosynthetic bacterium, the four components mentioned above were separated with good resolution. Furthermore, the use of this method would provide a fast, sensitive and specific method for monitoring the administration of delta-aminolevulinic acid in photodynamic cancer therapy, for the measurement of delta-aminolevulinic acid dehydratase activity in erythrocytes, and for testing the delta-aminolevulinic acid assay and for impurities in drug formulation.
Kim
J N
JN
Faculty of Chemical Engineering, Chonnam National University, Gwangju, South Korea.
Yun
J S
JS
Ryu
H W
HW
D000622:Aminolevulinic Acid / Q000032:analysis*; D002138:Calibration; D003470:Culture Media / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005998:Glycine / Q000032:analysis*; D007982:Levulinic Acids / Q000032:analysis*; D011162:Porphobilinogen / Q000032:analysis*; D012242:Rhodobacter sphaeroides / Q000737:chemistry
D003470:Culture Media; D007982:Levulinic Acids; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; C032246:levulinic acid; D005998:Glycine