[Analysis on HLA-E polymorphism in Shanghai Han population].
OBJECTIVE
To investigate the distribution of HLA-E alleles in Chinese Han in Shanghai area.


METHODS
HLA-E alleles were assigned by using a PCR/SSO method in 201 random healthy individuals from Shanghai region. HLA-A,-B antigens typing was carried out with NIH standard microlymphocytoxicy method.


RESULTS
Three alleles of HLA-E could be detected in this population. The E*0101 is the most common allele with a frequency of 42.29%, followed by E*01032 and E*01031 with 32.84% and 24.88% respectively. No E*0102 and E*0104 could be detected in all of the individuals. The analysis of linkage on two loci between HLA-E and HLA-A or -B showed that no significant difference could be found between expected frequencies and observed frequencies excepting B15/E*01032 and A2/E*01032.


CONCLUSIONS
The allele frequencies of HLA-E are 42.29% for E*0101, 24.88% and 32.84% for E*01031 and E*01032 respectively in Shanghai Chinese. No extensive linkage disequilibrium was found between HLA-E and HLA-A or -B locus.
Zhao
L
L
Shanghai Second Medical University, Shanghai Institute of Immunology, Shanghai, 200025 P.R.China. Lian_Fan@online.sh.cn
Fan
L
L
Yang
J
J
Yao
F
F
Xu
L
L
D000483:Alleles; D002681:China; D004247:DNA / Q000235:genetics; D005787:Gene Frequency; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D000097625:HLA-E Antigens
D004247:DNA; D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens