Influence of various biological matrices (plasma, blood microdialysate) on chromatographic performance in the determination of beta-blockers using an alkyl-diol silica precolumn for sample clean-up.
A HPLC column-switching system with LiChrospher RP-8 ADS precolumn was applied for the determination of beta-blockers (atenolol, pindolol, propranolol) in human plasma. The influence of biological matrices on the changes of the chromatographic parameters such as retention time, peak symmetry, area and selectivity were investigated. After injection of 5 ml plasma a decrease of retention times of the analytes was observed of up to 25% and an increase of asymmetry factors of up to 5%. Peak areas and selectivities were not changed. The observed effect could indicate changes of chromatographic performance caused by contributions of the analytical column or the ADS precolumn. The experiments with microdialysis excluded the contribution of the analytical column. A detailed investigation of experiments have been discussed in this paper.
Mislanová
C
C
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic. mislan@upkm.sk
Hutta
M
M
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D006801:Humans; D017551:Microdialysis; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000319:Adrenergic beta-Antagonists