Modified Pseudomonas agar: new differential medium for the detection/enumeration of Pseudomonas aeruginosa in mineral water.
Pseudomonas aeruginosa has been implicated as a foodborne and waterborne pathogen and is now considered a primary infectious agent. In the present study, the survival of P. aeruginosa inoculated in mineral water was evaluated by drop counts on Pseudomonas Agar Base (PAB), PAB with CN supplement X107, PAB with cetrimide, PAB with nalidixic acid, and these media with added FeSO(4). Initial counts, before starvation, were the same in all media tested. Following this period, P. aeruginosa became sensitive to PAB with added cetrimide. The addition of FeSO(4) did not improve the recovery of stressed P. aeruginosa but gave colonies a typical dark brown colour being easily differentiated from other species that can grow at 42 degrees C. The modified Pseudomonas agar medium was also tested with several P. aeruginosa strains, other species of Pseudomonas, and other genera. Only P. aeruginosa strains (pyocyanin positive) produced the typical colonies. Our results demonstrate that Pseudomonas agar with ferrous sulphate, used for the differentiation of P. aeruginosa colonies, and nalidixic acid, used as an inhibitor of Gram-positive bacteria, might be a useful medium for the detection of injured P. aeruginosa in mineral water.
Ramalho
Rita
R
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua António Bernardino de Almeida, 4200-072, Porto, Portugal. rramalho@morango.esb.ucp.pt
Cunha
Joaquim
J
Teixeira
Paula
P
Gibbs
Paul A
PA
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003470:Culture Media; D008900:Mineral Waters / Q000382:microbiology*; D009268:Nalidixic Acid / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D003470:Culture Media; D008900:Mineral Waters; D009268:Nalidixic Acid