[Study on preventive and therapeutic effect of radix salviae miltiorrhizae on recurrence and metastasis of liver cancer].
OBJECTIVE
To study the inhibitory effect of Radix Salviae Miltiorrhizae (RSM) on adhesive and invasive ability of SMMC-7721 liver cancer cells, and on the metastasis and recurrence on post-hepatectomy liver cancer in nude mice.


METHODS
Effect of RSM on SMMC-7721 cell membrane intercellular adhesive molecule-1 (ICAM-1) expression was observed by immunofluorescence flow cytometry; effect on invasive ability and of SMMC-7721 cell and the detachment of which attached to fibronectin (FN) by cell migration experiment; effcet on adhesion of SMMC-7721 cell with FN by MTT method and effect on adhesion between 7721-7721, 7721-lymphocyte and 7721-endothelial cell by cell adhesion experiment. LCI-D20 human liver cancer metastasis model, after hepatectomy, was used to observe the effect of RSM on recurrence and metastasis of liver carcinoma in nude mice.


RESULTS
ICAM-1 expression in SMMC-7721 cells incubated with RSM was significantly lower than that in cells did not treated with RSM. RSM could inhibit the invasive ability of SMMC-7721 cell and made the cells already attached to FN exfoliated. It could also inhibit the adhesion of 7721-7721, 7721-lymphocyte and 7721-endothelial cell. And RSM showed preventive and therapeutic effect on intrahepatic and remote metastasis/recurrence of early and late human post-hepatectomy liver cacer in nude mice.


CONCLUSIONS
RSM could inhibit the invasion and adhesion of SMMC-7721 cell and could also prevent and inhibit metastasis and recurrence of human liver cancer after hepatectomy in nude mice.
Sun
J
J
Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai (200032).
Zhou
X
X
Liu
Y
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis / Q000517:prevention & control; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control*; D010936:Plant Extracts; D027561:Salvia miltiorrhiza; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D018799:Intercellular Adhesion Molecule-1