[The role of basic fibroblast growth factor on expression of mineralization of periodontal ligament cell population and clones].
OBJECTIVE
To determine the effect of basic fibroblast growth factor (bFGF) on mineraliztion of PDL cell clone.


METHODS
The PDL tissue that derived from three human donors was cultured and cells were cloned. The mineralizing ability were detected with or without bFGF treatment in the presence of dexamethasone. Cloned progenitor populations were assayed for their constitutive capacity to bind CAP and express ALP.


RESULTS
The results show that bFGF increases clone mineralization (11%), but not in PDL cell population. The cell clones that mineralized only in the presence of bFGF have higher CAP binding ability and lower ALP expression compared with other mineralized clone cells.


CONCLUSIONS
bFGF enhances mineralizing expression of PDL clone. The clones that mineralized only with bFGF treatment are different cell types from the bFGF-undependent mineralized clones.
Liu
H
H
Department of Stomatology, PLA General Hospital, Beijing 100853.
Sandu
P
P
Yuan
Z
Z
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D002113:Calcification, Physiologic; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002999:Clone Cells; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006801:Humans; D008297:Male; D010513:Periodontal Ligament / Q000166:cytology*; D014074:Tooth Calcification
D015815:Cell Adhesion Molecules; C433705:cementum attachment protein, 55-kDa; D016222:Fibroblast Growth Factor 2; D000469:Alkaline Phosphatase