[Effect of immunizing rats with a serotonin-bovine serum albumin conjugate on the structure of diurnal sleep and electrical activity of the brain].
Rats were immunized with bovine serum albumin conjugated with 5-hydroxytriptamine (5-HT). Two injections with a 2-week interval were carried out. At first, rats were injected subcutaneously (2 mg/kg, 0.25 ml) with Freund's adjuvant complete. The second injections (10 mg/kg intraperitoneally) was made 2 weeks later without the adjuvant. One week after the first antigen injection, there were a decrease in the relative power of the electrical activity of the caudate putamen, neocortex, and amygdala in the delta 2 and theta 2 bands and its increase in the alpha and beta-ranges. The number and duration (total and mean) of REM sleep episodes as well as REM sleep contribution in the total sleep duration decreased. These findings testify to a reduced functional activity of the central serotonergic system. Four-five weeks after the first injection, a tendency to a recovery of the bio-electrical spectral pattern and diurnal sleep structure was revealed. Five weeks after the first antigen injection, the titre of antibodies to 5-HT was 1:32-1:64.
Pankova
N B
NB
Institure of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow.
Vertrilé
L A
LA
Basharova
L A
LA
Krupina
N A
NA
Kryzhanovskiĭ
T N
TN
D000818:Animals; D001921:Brain / Q000502:physiology*; D002940:Circadian Rhythm; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000008:administration & dosage*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D012890:Sleep
D012710:Serum Albumin, Bovine; D012701:Serotonin
Vliianie immunizatsii krys kon''iugatom serotonin-bychiĭ syvorotochnyĭ al'bumin na strukturu dnevnogo sna i élektricheskuiu aktivnost' mozga.