Effect of polyacetylenes on the neurite outgrowth of neuronal culture cells and scopolamine-induced memory impairment in mice.
Polyacetylenic alcohols and their linoleates isolated from Panax ginseng C. A. MEYER and Cirsium japonicum DC., of which the lipophilic extracts had been found to affect the neuritogenesis of cultured paraneurons, were demonstrated to have a significant neuritogenic effect on PC12h and Neuro2a cells. Panaxynol and the acetylenic triol in particular were highly efficient at concentrations > or = 2 microm. Panaxynol (20 mg/kg/d, i.p., for 3 d) was confirmed to improve scopolamine-induced memory deficit in mice (Y-maze task). It is suggested that the promotion of neuritogenesis in cultured paraneurons by the addition of panaxynol is related its ability to improve memory deficits in animals.
Yamazaki
M
M
Department of Biodynamics, Faculty of Pharmacy, Hokuriku University, Kanazawa, Ishikawa, Japan. yamazaki@hokuriku-u.ac.jp
Hirakura
K
K
Miyaichi
Y
Y
Imakura
K
K
Kita
M
M
Chiba
K
K
Mohri
T
T
D000114:Acetylene / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy*; D051379:Mice; D018727:Muscarinic Antagonists / Q000633:toxicity; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells; D005894:Panax; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D011108:Polymers / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use*; D053279:Polyynes; D051381:Rats; D012601:Scopolamine / Q000633:toxicity*
D018727:Muscarinic Antagonists; D010936:Plant Extracts; D011108:Polymers; D053279:Polyynes; D012601:Scopolamine; D000114:Acetylene