The transforming activity of tumour cell DNA.
Transfection of DNA isolated from a subset of human tumour cells was shown in the early 1980s to be capable of inducing transformation in NIH3T3 cells. This review summarizes the approaches and results of the efforts to measure the transforming activity of genomic DNA and discusses the results in the light of current concerns about the potential hazard associated with DNA contamination of biological material produced by cultured transformed cells.
Blair
D G
DG
Oncogene Mechanisms Section, NIH/NCI/DBS/BRL, Frederick Cancer Research and Development Center, MD 21702-1207, USA.
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000502:physiology*; D006801:Humans; D051379:Mice; D014162:Transfection
D004273:DNA, Neoplasm