[Analysis on the genotype of measles virus epidemic strains in Dongguan city, Guangdong province in 2000].
OBJECTIVE
To analyze the genotype of the measles virus epidemic strains in Dongguan according to the patients suffered measles in 2000.


METHODS
The saliva of 55 patients with acute measles symptoms were collected and detected with RT-PCR. The PCR products of the 5 samples were sequenced and analyzed with the software DNASTAR.


RESULTS
The positive detection rate was 87.3%, whereas the positive rate of anti MV-IgM in serum was only 70.9%. 68.8% of the saliva samples of patients with anti MV-IgM negative results showing MV RNA positive. The 293 bp fragment of N gene and 194 bp fragment of M gene were amplified from the vaccine virus and the specimens. The sequences of the epidemic strains were identical in both M gene and N gene.


CONCLUSIONS
These results suggested that the outbreak came from a single MV strain. The epidemic strain was obviously different from the standard strains discovered in other countries of the world (the deviation were 1.4%-11.7%) that might belong to H genotype.
Zhu
J
J
Dongguan City Health and Anti-epidemical Station, Dongguan 523000, China.
Peng
Z
Z
Lei
M
M
D002681:China / Q000453:epidemiology; D005838:Genotype; D006801:Humans; D008457:Measles / Q000453:epidemiology / Q000821:virology*; D008459:Measles virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral