Changes in plasma antiproteases in paraquat poisoned rats using micro two-dimensional electrophoretic analysis.
Rats were subjected to subacute paraquat (PQ) exposure (7 mg/kg sc daily for 6-9 administrations). They were divided into a group that died from subacute toxicity, a group that recovered from the subacute toxicity and progressed to pulmonary fibrosis, and a group that showed no effects from the PQ. The rats with subacute fatal toxicity had a remarkable increase in a2M-1. Those which progressed to pulmonary fibrosis had remarkable decreases in a2M-2, a2M-3, and a2M-4 and a1AT. The rats that showed no effects from the PQ had relative increases in all molecular species of a2M. Increases in major a2M molecular species, a2M-1 and a2M-4, and a minor molecular species, a2M-3, were characteristically observed.
Kageyama
K
K
Department of Veterinary Pharmacology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Akahori
F
F
Sakaguchi
K
K
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006540:Herbicides / Q000506:poisoning*; D007279:Injections, Subcutaneous; D008297:Male; D010269:Paraquat / Q000506:poisoning*; D011480:Protease Inhibitors / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000662:veterinary; D051381:Rats; D017208:Rats, Wistar
D006540:Herbicides; D011480:Protease Inhibitors; D010269:Paraquat