title: [DNA-abzymes and their clinical significance in systemic lupus erythematosis].
abstract: OBJECTIVE
  To evaluate occurrence of DNA-abzymes with catalytic (DNA-hydrolysing) and cytotoxic properties in patients with systemic lupus erythematosus (SLE) for examination of clinical value of DNA-abzymes in diagnosis of autoimmune syndrome and apoptosis level in different variants of immunopathology.
  
  
  METHODS
  The study group consisted of patients with verified SLE diagnosis (n = 120). They were compared to 72 patients with rheumatoid arthritis (RA), 82 patients with scleroderma systematica (SS), 60 patients with discoid lupus erythematosus (DLE), 88 patients with focal scleroderma (FS) and 198 autoimmune uveitis (AU) patients. 128 donors served control. Catalytic and cytotoxic activity of DNA-abzymes were determined by methods of molecular biology and enzymology. All the patients were examined for blood levels of IgG, IgM and IgA, anti-DNA, anti-Sm and other IgG-autoantibodies, CIC titers, phagocyting activity, content of main D-cell subpopulations. Key immunoregulatory indices were also estimated.
  
  
  RESULTS
  DNA-abzymes were detected more often in SLE and RA patients. In SLE, catalytic and cytotoxic activities of DNA-abzymes reached their maximum. There was a correlation with leading clinicoimmunological signs of SLE. The disease was most severe with apparent immunopathology in patients with maximal catalytic and cytotoxic activity of DNA-abzymes. With lowering cytotoxic activity of DNA-abzymes more patients demonstrate low SLE activity without severe organic lesions and alleviated symptoms of immunopathology.
  
  
  CONCLUSIONS
  An important role of DNA-abzymes in pathogenesis of SLE is shown. They are also valuable tools in diagnosis of various clinicoimmunological variants of the disease.
authors.forename: S V
authors.initials: SV
authors.lastname: Suchkov
authors.forename: A G
authors.initials: AG
authors.lastname: Gabibov
authors.forename: Z S
authors.initials: ZS
authors.lastname: Alekberova
authors.forename: N V
authors.initials: NV
authors.lastname: Gnuchev
mesh_terms: D017209:Apoptosis / Q000276:immunology; D001323:Autoantibodies / Q000097:blood*; D002384:Catalysis; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D004247:DNA
vernacular_title: DNK-abzimy i ikh klinicheskoe znachenie pri sistemnoĭ krasnoĭ volchanke.