title: Effect on the healing of periapical perforations in dogs of the addition of growth factors to calcium hydroxide.
abstract: The purpose of this study was to investigate the effect of the addition of platelet-derived growth factor-BB and insulin-like growth factor-I to calcium hydroxide in the repair of apical perforations in dogs. Fifty-one premolar teeth of four beagle dogs were used. After developing periapical lesions root apices were artificially perforated. The teeth were divided into the three groups: group 1, the apical perforations were not sealed; group 2, the perforated areas were obturated with calcium hydroxide; and group 3, calcium hydroxide plus growth factors was applied to the sites of perforation. All canals were filled by a lateral condensation technique. Animals were killed 12 wk later, and sections were hematoxylin & eosin-stained and immunostained for osteonectin. The amount of inflammation was evaluated histomorphologically. The one-way ANOVA test demonstrated that the three groups were significantly different from one another. In group 3 there was no inflammatory reaction of apical tissue, and the connective tissue adjacent to the newly formed hard tissue was strongly immunostained for osteonectin. Most sections in group 1 showed no apical healing. Moderate healing was found in group 2. In conclusion the combination of platelet-derived growth factor-BB and insulin-like growth factor-I with calcium hydroxide improved healing of apical perforation in dogs.
authors.affiliation: Department of Conservative Dentistry, Asan Medical Center, Seoul, Korea.
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.forename: B
authors.initials: B
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Yoon
mesh_terms: D016301:Alveolar Bone Loss / Q000188:drug therapy / Q000209:etiology; D000704:Analysis of Variance; D000818:Animals; D000077214:Becaplermin; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004285:Dogs; D004338:Drug Combinations; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology* / Q000627:therapeutic use; D015676:Osteonectin / Q000502:physiology; D010482:Periapical Abscess / Q000188:drug therapy / Q000209:etiology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology* / Q000627:therapeutic use; D020574:Proto-Oncogene Proteins c-sis; D012388:Root Canal Irrigants / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000009:adverse effects; D019227:Tooth Apex / Q000293:injuries*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004338:Drug Combinations; D015676:Osteonectin; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012388:Root Canal Irrigants; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I; D002126:Calcium Hydroxide