[Identification of postoperative M. chelonae infection outbreak by polymerase chain reaction of 16s-23s ribosomal DNA spacer sequence].
OBJECTIVE
To determine the pathogen of the infection outbreak in a hospital in Shenzhen at gene level with molecular biological technique, and to establish a rapid identification method of M. chelonae subsp. abscessus by polymerase chain reaction technique.


METHODS
A single pair of primers and oligonucleotides probe of M. chelonae subsp. abscessus were designed, according to the sequence of mycobacterium of 16s-23s ribosomal DNA spacer sequences. 53 clinical strains were amplified by polymerase chain reaction, then dot-blot hybridization of PCR product was made. On the basis of this study, 259 lesion, tissue and normal tissue were amplified by 16s-23s rDNA PCR.


RESULTS
380 bp PCR product for 53 clinical strains was yielded by 16s-23s rDNA PCR, in the meantime, the specific hybridization dot appeared. The PCR positive rate of 259 specimens was 60.6%.


CONCLUSIONS
The PCR products and dot-blot hybridization showed that the pathogen of the infection outbreak was M. chelonae subsp. abscessus at gene level. The 16s-23s rDNA PCR investigative system is sensitive and specific, which can identify M. chelonae subsp. abscessus.
Hu
Q
Q
Shenzhen Sanitary & Anti-Epidemic Station, Shenzhen 518020.
Li
L
L
Zhuang
Y
Y
D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D016926:Mycobacterium chelonae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S