title: Site-directed mutagenesis facilitated by DpnI selection on hemimethylated DNA.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, WA, USA.
authors.forename: Fusheng
authors.initials: F
authors.lastname: Li
authors.forename: James I
authors.initials: JI
authors.lastname: Mullins
mesh_terms: D019175:DNA Methylation; D017931:DNA Primers; D004277:DNA, Single-Stranded / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D007202:Indicators and Reagents; D008967:Molecular Biology / Q000379:methods; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific