Possible misidentification of Bacteroides sp., probably B. ureolyticus as Taylorella equigenitalis: implications for the laboratory diagnosis of CEM.
A wild-type isolate with similar morphological and phenotypic properties to Taylorella equigenitalis, the causative bacterial agent of contagious equine metritis (CEM), was referred for molecular identification by PCR amplification of the 16S rRNA gene. A species-specific PCR failed to yield a product compatible with that of T. equigenitalis. The direct sequencing of the universal 16S rRNA PCR amplicon suggested the presence of a Bacteroides sp., probably Bacteroides ureolyticus, with no consequent effects on the movement and transportation of the animal. Adoption of such a molecular means of identification through sequencing may aid in the identification of the atypical forms of Taylorella equigenitalis, as recently described, as well as differentiating this species from Taylorella asinigenitalis.
Moore
J E
JE
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital. jemoore@niphl.dnet.co.uk
Millar
B C
BC
Xu
J
J
Buckley
T C
TC
D000818:Animals; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis; D004716:Endometritis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D013045:Species Specificity; D020625:Taylorella equigenitalis / Q000235:genetics / Q000302:isolation & purification*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S