[Comparative study of the BACTEC 9000 MB system and Lowenstein Jensen media for mycobacterial culture].
An automated system for mycobacteria culture, BACTEC 9000 MB, was compared with Lowenstein Jensen culture. On a total of 4,484 pulmonary and extrapulmonary human clinical samples, 126 (2.8%) were positive for mycobacteria on Lowenstein Jensen (LJ) medium; 105 (2.34%) were identified as Mycobacterium tuberculosis and 39 (1.10%) as non tuberculosis mycobacteria. The mean time of detection of Mycobacterium tuberculosis on the 131 positive samples was reduced to approximately ten days with BACTEC 9000 MB compared to the LJ (17.6 versus 27.38 days). Through the results of this comparative study, we confirmed that BACTEC 9000 MB is a more efficient system than LJ for culture detection of all mycobacteria from various sites samples.
Soulier Majidi
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Centre hospitalier intercommunal de Créteil, laboratoire de microbiologie hygiène, 40, avenue de Verdun, 94000 Créteil, France.
Ciupek
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Malbrunot
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Baufine Ducrocq
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D001322:Autoanalysis; D003470:Culture Media; D006801:Humans; D008168:Lung / Q000382:microbiology; D009161:Mycobacterium / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
D003470:Culture Media
Etude comparative du système BACTEC 9000 MIB et du milieu de Lowenstein Jensen pour la culture des mycobactéries.