title: Plasmodium falciparum activates endogenous Cl(-) channels of human erythrocytes by membrane oxidation.
abstract: Intraerythrocytic survival of the malaria parasite Plasmodium falciparum requires that host cells supply nutrients and dispose of waste products. This solute transport is accomplished by infection-induced new permeability pathways (NPP) in the erythrocyte membrane. Here, whole-cell patch-clamp and hemolysis experiments were performed to define properties of the NPP. Parasitized but not control erythrocytes constitutively expressed two types of anion conductances, differing in voltage dependence and sensitivity to inhibitors. In addition, infected but not control cells hemolyzed in isosmotic sorbitol solution. Both conductances and hemolysis of infected cells were inhibited by reducing agents. Conversely, oxidation induced identical conductances and hemolysis in non-infected erythrocytes. In conclusion, P.falciparum activates endogenous erythrocyte channels by applying oxidative stress to the host cell membrane.
authors.affiliation: Department of Physiology, University of Tübingen, Gmelinstrasse 5, D-72076 Tübingen, Germany. stephan.huber@uni-tuebingen.de
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Huber
authors.forename: Anne-Catrin
authors.initials: AC
authors.lastname: Uhlemann
authors.forename: Nikita L
authors.initials: NL
authors.lastname: Gamper
authors.forename: Christophe
authors.initials: C
authors.lastname: Duranton
authors.forename: Peter G
authors.initials: PG
authors.lastname: Kremsner
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000097:blood*; D004553:Electric Conductivity; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D018408:Patch-Clamp Techniques; D010963:Plasmodium falciparum / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D018118:Chloride Channels