[Experimental study of tissue engineered bone with coralline hydroxyapatite as scaffolds].
OBJECTIVE
To investigate the feasibility of coralline hydroxyapatite (CHA) as scaffolds in bone tissue engineering.


METHODS
The bone marrow stromal cells from 4-month New Zealand rabbits were harvested and cultured in vitro. After multiplied, dexamethasone was used to promote the osteoblastic phenotype of the cells. The cells were harvested and then seeded into CHA. By means of tissue engineering technique, osteoblastic cells/CHA complex were formed. The complex were implanted subcutaneously in nude mice. The CHA alone was implanted as control. Bone regeneration was assessed 6, 8 weeks after implantation by histological and roentgenographic analysis.


RESULTS
After six weeks of implantation, x-ray film showed high-density signal, osteoid tissue formed under histological examination. Large amount of new bone were formed and connected to trabecularism 8 weeks after implantation in the experimental group. While in the control group, there were no new bone formation, but amount of fiber tissue grew into the pore of CHA 8 weeks after implantation.


CONCLUSIONS
CHA may be used as a good scaffold material for bone tissue engineering.
Shi
P L
PL
Stomatological College, Fourth Military Medical University, Xi'an Shangxi, P. R. China 710032.
Gu
X M
XM
Chen
F L
FL
D000818:Animals; D015519:Bone Density; D001854:Bone Marrow Cells / Q000166:cytology*; D001857:Bone Matrix / Q000166:cytology; D002478:Cells, Cultured; D002516:Ceramics; D006882:Hydroxyapatites; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts; D010012:Osteogenesis; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D023822:Tissue Engineering
D006882:Hydroxyapatites; C119955:coralline hydroxyapatite