title: Histometric evaluation of the effect of nicotine administration on periodontal breakdown: an in vivo study.
abstract: The present study investigated the effect of nicotine administration on periodontal breakdown resulting from ligature-induced periodontitis in rats. Twenty adult male Wistar rats were used. After anesthesia, a mandibular first molar was randomly assigned to receive a cotton ligature in the sulcular area while the contralateral tooth was left unligated. The animals were randomly assigned to one of the following treatments. of daily intraperitoneal injections: A - saline solution, B -0.37 mg of nicotine kg, C -0.57 mg of nicotine kg and D -0.73 mg of nicotine/kg. Thirty days later, the animals were sacrificed and the specimens routinely processed for serial decalcified sections. Statistical analysis (ANOVA) revealed greater bone loss (p<0.05) in the ligated teeth of animals which received nicotine (groups B/C D) than in the ligated teeth of animals which received saline solution (group A). In addition, a dose-dependent response was observed among the nicotine groups. A negative effect of nicotine was observed in the unligated teeth of the experimental groups (p<0.05). Therefore, daily administration of nicotine enhanced, in a dose-dependent manner, the effects of local factors in producing periodontal breakdown. Furthermore, the nicotine seemed to have a direct deleterious effect on the periodontal tissues.
authors.affiliation: Dept. of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, UNICAMP, Sao Paulo, Brazil. nociti@fop.unicamp.br
authors.forename: F H
authors.initials: FH
authors.lastname: Nociti
authors.forename: G R
authors.initials: GR
authors.lastname: Nogueira-Filho
authors.forename: V A
authors.initials: VA
authors.lastname: Tramontina
authors.forename: M A
authors.initials: MA
authors.lastname: Machado
authors.forename: S P
authors.initials: SP
authors.lastname: Barros
authors.forename: E A
authors.initials: EA
authors.lastname: Sallum
authors.forename: A W
authors.initials: AW
authors.lastname: Sallum
mesh_terms: D016301:Alveolar Bone Loss / Q000139:chemically induced* / Q000473:pathology; D000704:Analysis of Variance; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007274:Injections, Intraperitoneal; D008026:Ligation; D008297:Male; D009538:Nicotine / Q000008:administration & dosage / Q000633:toxicity*; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D010518:Periodontitis / Q000209:etiology; D011897:Random Allocation; D051381:Rats; D018709:Statistics, Nonparametric
chemical_list: D009538:Nicotine