[The effect of non-surgical periodontal and adjunctive minocycline-HCL treatments on collagenase activity].
OBJECTIVE
To evaluate the effect of scaling and root planing combined with local application of 2% minocycline hydrochoride ointment(Periocline) on the level of collagenase activity releasing from PMNs in GCF.


METHODS
This was a randomized, single-blinded, split mouth design study. The target sites of 31 patients with moderate to severe periodontitis (PD > 5 mm) was randomized by left-side and right-side into one of the two groups (the test group and the control group). Subsequently each patient was scheduled for two appointments for the full-month supra-gingival scaling and oral hygiene instruction. At baseline all patients received subgingival scaling and root planing and were followed by 4-time applications of Periocline with one-week interval for totally four weeks in the test group. All patients were taken GCF for measuring collagenase by the method of Nakashima et al (1996) and were examined based on four parameters, including plaque index, pocket depth, attachment loss and bleeding on probing.


RESULTS
Four weeks after root planing with 4-time applications of Periocline, all clinical parameters and active collagenase in GCF decreased significantly in the test groups[(593 +/- 112) mU/sample vs (311 +/- 98) mU/sample, P < 0.001]. On the other hand, simple root-planing therapy had limited effect on the active collagenase[(611 +/- 123) mU/sample vs (523 +/- 127) mU/sample], although all clinical parameters were also reduce significantly. Interestingly, seven weeks later, gingival inflammation and the active collagenase were reduced more significantly than those after four-week root planing in both groups[Test: (311 +/- 98) mU/sample vs (207 +/- 57) mU/sample;


METHODS
(523 +/- 127) mU/sample vs (345 +/- 117) mU/sample, P < 0.001]. At eleven week, gingival inflammation and the level of collagenase activity were rebounded in the control group[(467 +/- 108) mU/sample]. However, in the test group they were kept in lower level[(213 +/- 121) mU/sample]. The level of collagenase activity showed significantly positive correlation with GCF volumes and AL in two groups.


CONCLUSIONS
These results indicate that local application of 2% minocycline hydrochoride ointment may effectively inhibit the level of active collagenase from PMNs and may inhibit connective tissue breakdown by inhibiting neutrophil collagenase.
Geng
S
S
Department of Periodontology, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Cao
C
C
Chen
Z
Z
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D017364:Collagenases / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008911:Minocycline / Q000627:therapeutic use*; D010518:Periodontitis / Q000188:drug therapy* / Q000201:enzymology; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D017364:Collagenases; D008911:Minocycline