Melatonin shortens the survival rate of Ehrlich ascites-inoculated mice.
OBJECTIVES Pineal gland may have a role in organism's protection against cancer. Melatonin as well as still unidentified low-weight molecular pineal substance(s) have been reported to have growth inhibitory effect on different tumor cells. We tested the influence of melatonin and of a bovine pineal extract on the survival rate of AKR mice inoculated with Ehrlich ascites. The tumor is known to have an accelerated development after pinealectomy.


METHODS
Male AKR mice, kept under a 14/10 hours - Light /Dark cycle, were inoculated intraperitoneally (i.p.) with 1.5x 10(6) Ehrlich ascites cells. On day three after inoculation the animals were divided in three groups (n=10). Each animal received i.p. daily (20.00H), until their death, 250 microl of solution containing melatonin (250 microg), pineal extract (equivalent of 1 bovine pineal gland) or saline.


RESULTS
The average survival rate of the animals treated with melatonin was shorter (14.8+/-2.23 days) compared to control animals (21.9+/-2.21 days)(p=0.01). The animals treated with the pineal extract had a longer survival rate (22.6 +/- 1.8 days) but not statistically significant. The pineal extract was not available for testing at higher doses.


CONCLUSIONS
In our model, melatonin had a deleterious effect on the survival rate raising the question whether it is correct to assume that the hormone shows lack of adverse reactions.
Catrina
S B
SB
Dept. Endocrinology II, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania. sergiu-bogdan.catrina@molmed.ki.se
Curca
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E
Catrina
A I
AI
Radu
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Coculescu
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M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000401:mortality*; D002417:Cattle; D002457:Cell Extracts / Q000494:pharmacology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D008806:Mice, Inbred AKR; D009368:Neoplasm Transplantation; D010870:Pineal Gland / Q000502:physiology; D015996:Survival Rate
D000975:Antioxidants; D002457:Cell Extracts; D008550:Melatonin