[Chemotactic response of polymorphonuclear neutrophil to FMLP and IL-8 in rapidly progressive periodontitis patients].
OBJECTIVE
It has been reported that 48%-85% of rapidly progressive periodontitis (RPP) are associated with a defect in polymorphonuclear neutrophil (PMN) chemotactic function. However, some reports failed to detect any defect in PMN chemotaxis in RPP patients. Further, the influence of race on neutrophil function has been suggested. The purpose of the present study was to determine the PMN chemotactic function in Chinese RPP patients.


METHODS
Neutrophils were obtained from heparinized peripheral blood of 16 RPP and 14 periodontally healthy control subjects matched for age and sex. Cells were isolated by Percoll discontinuous density gradient centrifugation. Chemotaxis was evaluated using modified Boyden chamber. (N-formyl-methionyl-leucyl-phenylalanine, FMLP) (10(-8) mol/L) and IL-8 (100 micrograms/L) which is a more specific neutrophil chemokines were used in this study. The results were determined by staining the filters followed by visual counting under microscopy at 600 x. Data was analyzed using the Students' t test and the value of RPP was compared with that of control subjects(> mean +/- 2s or < mean +/- 2s).


CONCLUSIONS
The results showed all RPP patients had normal PMN chemotactic behavior.
Liu
R
R
School of Stomatology, Beijing Medical University, Beijing 100081.
Cao
C
C
D000328:Adult; D002634:Chemotaxis, Leukocyte; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology*
D016209:Interleukin-8; D009240:N-Formylmethionine Leucyl-Phenylalanine