[Therapeutic effects of adenovirus-bcl-xs gene to the ascites tumor of nude mice model of human ovarian carcinoma].
OBJECTIVE
To investigate the effects of adenovirus-bcl-xs gene on the ascites tumor growth inhibition and survival rate of nude mice with human ovarian carcinoma transplanted intraperitoneally.


METHODS
Making an adenovirus-bcl-xs gene vector infected in JH293T cell and reproduced in it. After having detected the inhibitory potential of adenovirus-bcl-xs gene on NUTU-19 cell we use it to transfer intraperitoneally to ascites tumor model of human ovarian carcinoma transplanted in nude mice. Detected the ascites formation, the survival time and survival rate of nude mice with the human ascites tumour. The weight and toxic-adverse systemically effects of nude mice was observed and morphology of adenovirus was observed by electromicroscope and the gene expression was detected by immunocell chemistry.


RESULTS
The adenovirus-bcl-xs gene could reproduce in JH293T cell and had inhibitory potential on NUTU-19 cell, the survival time of nude mice was longer and the survival rate was higher, and the time of ascites formation was retarted. There was no obvious alternation in the weight and systemic toxic-adverse effects observed.


CONCLUSIONS
The data suggests that the transfer of adenovirus-bcl-xs gene to the ascites tumour of nude mice with human ovarian carcinoma could improve the survival rate of nude mice and retard the time of ascites formation. It may be a useful method of gene therapy in the treatment of ovarian carcinoma.
Chen
A
A
Department of Obstetrics and Gynecology, Affiliated Hospital, Medical Colledge of Qingdao University, Qingdao 266003, China.
Peng
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Z
Wang
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H
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000628:therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D051020:bcl-X Protein
C494811:BCL2L1 protein, human; C494812:Bcl2l1 protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein