title: Histochemical study of transmyocardial laser channels in dogs.
abstract: OBJECTIVE
  Laser-induced myocardial injury was investigated in a canine transmyocardial laser revascularization model.
  
  
  METHODS
  A total of 23 channels were created in the left ventricles of 5 animals with a CO2 laser. Four animals were sacrificed immediately and one at week 2. Lactate dehydrogenase and succinate dehydrogenase in channel regions were examined with a Nitro-BT method staining.
  
  
  RESULTS
  Immediately after the procedure, laser channels and surrounding injured myocardium could be stratified from the center to the border into 5 concentric zones, i.e., vaporized zone, carbonized zone, enzyme-disappeared zone, enzyme-decreased zone and enzyme-concentrated zone. Two weeks later, the phenomenon disappeared.
  
  
  CONCLUSIONS
  Immediately after transmyocardial revascularization, there is a ring of enzyme-concentrated zone at the outermost region of injured myocardium. Two weeks later, enzyme activities of reversibly injured myocytes appear to have recovered.
authors.affiliation: Department of Cardiology, Third Hospital, Beijing University Medical School, Beijing 100083, China. leilili@china.com
authors.forename: L
authors.initials: L
authors.lastname: Lei
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Mao
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Bai
mesh_terms: D000818:Animals; D004285:Dogs; D006335:Heart Injuries / Q000209:etiology*; D006352:Heart Ventricles / Q000201:enzymology / Q000293:injuries; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053685:Laser Therapy / Q000009:adverse effects*; D009204:Myocardial Revascularization / Q000009:adverse effects*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors
chemical_list: D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase