Attempt to differentiate Leishmania (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis using the SSR-PCR technique.
The ability to differentiate reference strains of Leishmania (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis was evaluated using the simple sequence repeat polymerase chain reaction (SSR-PCR) technique. This technique differentiates the Leishmania species, generating distinct DNA amplicon profiles. The SSR-PCR profiles were similar to but more reproducible than those produced by RAPD. SSR-PCR is presented as an alternative to other molecular methods for the differentiation of Leishmania species or strains.
Volpini
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AC
Laboratório de Parasitologia Celular Molecular do Centro de Pesquisas René Rachou/Fundacao Oswaldo Cruz, Belo Horizonte-MG, Brazil.
de Azeredo Passos
V M
VM
Romanha
A J
AJ
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics; D007892:Leishmania braziliensis / Q000145:classification / Q000235:genetics; D018334:Leishmania guyanensis / Q000145:classification / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics*; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D016054:DNA, Protozoan