[Apoptosis of pancreatic acinar and expression of TNF-alpha mRNA, IL-10 mRNA in rats with acute pancreatitis].
OBJECTIVE
To investigate the apoptosis of pancreatic acinar and the expression of TNF-alpha mRNA, IL-10 mRNA in pancreatic tissue of rats with acute pancreatitis.


METHODS
The model of acute edematous pancreatitis(AEP) was established in 20 rats and that of acute necrotizing pancreatits(ANP) in another 20 by injection of sodium taurocholate into the pancreatic duct. Another 10 normal rats were used as controls. At 12 hours after the induction of pancreatitis, 10 rats in each group were sacrificed. Serum and pancreatic TNF-alpha and IL-10 levels were measured. The expression of TNF-alpha and IL-10 mRNA in pancreas was detected and the apoptotic rate of pancreatic acinar determined.


RESULTS
The apoptotic rate of pancreatic acinar in normal, AEP and ANP groups was 2.98%, 17.29% and 8.39%, respectively. TNF-alpha and IL-10 levels increased after the induction of acute pancreatitis. The level of TNF-alpha was lower while that of IL-10 higher in AEP group than in ANP group. Transcription level of TNF-alpha mRNA in ANP group and that of IL-10 mRNA in AEP group were upregulated.


CONCLUSIONS
The transcription levels of TNF-alpha and IL-10 mRNA in pancreatic tissue of rats with acute pancreatitis are positively correlated with their levels in serum and pancreas, suggesting that the pancreas is the organ to release cytokines. The apoptotic rate of pancreatic acinar is negatively correlated with the severity of disease and apoptosis may be a benign response to pancreatic injury.
Jin
C
C
Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Ni
Q
Q
Zhang
Q
Q
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics*; D008297:Male; D010179:Pancreas / Q000473:pathology*; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10