Histogenesis of human colorectal adenomas and hyperplastic polyps: the role of cell proliferation and crypt fission.
BACKGROUND
The histogenesis of human colorectal hyperplastic polyps and colorectal adenomas is poorly understood even now.


METHODS
Human colorectal adenomas, hyperplastic polyps, and normal colorectal mucosae (patients with familial adenomatous polyposis and hereditary non-polyposis colorectal carcinoma were excluded) were obtained during colonoscopy and microdissected into individual crypts. Morphology, cell proliferation characteristics, and fission indices of crypts isolated from these lesions were then studied.


RESULTS
Crypts isolated from colorectal adenomas and colorectal hyperplastic polyps were significantly larger (p<0.001) than crypts from normal colorectal mucosae. Crypt fission was an uncommon event in normal colonic mucosae but common in crypts isolated from adenomas and hyperplastic polyps (p<0.001). Analysis of the distribution of mitoses suggested an upward expansion of the proliferation compartment in adenomas to the surface of the crypt with no reversal of proliferating cell distribution, as has previously been described.


CONCLUSIONS
Sporadic human colorectal adenomas and hyperplastic polyps grow by the process of crypt fission. Expansion of the proliferative compartment was demonstrated in crypts from adenomas, consistent with deregulation of cell cycle control.
Wong
W-M
WM
Histopathology Unit, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, UK. wmwongg@hku.hk
Mandir
N
N
Goodlad
R A
RA
Wong
B C Y
BC
Garcia
S B
SB
Lam
S-K
SK
Wright
N A
NA
D000236:Adenoma / Q000473:pathology*; D002455:Cell Division; D003111:Colonic Polyps / Q000473:pathology; D015179:Colorectal Neoplasms / Q000473:pathology*; D004210:Dissection / Q000379:methods; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry / Q000379:methods; D007413:Intestinal Mucosa / Q000473:pathology; D007417:Intestinal Polyps / Q000473:pathology*; D008938:Mitosis; D012004:Rectal Neoplasms / Q000473:pathology