[Effect of protein kinase C and NO-synthase inhibitors on the radiation-induced cytogenetic adaptive response in cultured Chinese hamster cells].
The effects of the serin-threonin kinase inhibitor--staurosporin and inhibitor of NO-synthase--L-NAME on the radiation-induced adaptive response were studied in fibroblasts of Chinese hamster in culture. We have shown that the adaptive response as measured by the reduction of radiation-induced chromosome aberrations was completely blocked in the presence of staurosporin as well as L-NAME. These inhibitors themselves did not increase the chromosome aberration frequency when cultures were irradiated with 3 Gy alone in the presence of inhibitors. Furthermore, L-NAME noticeably decreased the gamma-rays-induced level of the chromosome aberrations. Our study confirms the participation of protein kinase C in the induction of the adaptive response and first indicates the participation of NO-synthase in this response.
Gil'iano
N Ia
NIa
B.P. Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, St. Petersburg-Gatchina, 188350 Russia. giliano@omrb.pnpi.spb.ru
Bondarev
G N
GN
Bikineeva
E G
EG
Krasotskaia
G I
GI
Noskin
L A
LA
D000222:Adaptation, Physiological / Q000187:drug effects*; D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D019311:Staurosporine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011837:Radiation-Protective Agents; D019001:Nitric Oxide Synthase; D011493:Protein Kinase C; D019311:Staurosporine; D019331:NG-Nitroarginine Methyl Ester
Vliianie ingibitorov protein kinazy C i NO-sintazy na radiatsionno-indutsirovannyĭ tsitogeneticheskiĭ adaptivnyĭ otvet v kletkakh kitaĭskogo khomiachka v kul'ture.