Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis.
Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.
Reed
Carrie
C
Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA.
Sturbaum
Gregory D
GD
Hoover
Paul J
PJ
Sterling
Charles R
CR
D000818:Animals; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D004275:DNA, Ribosomal / Q000032:analysis; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S