Evaluation of three somatic genetic biomarkers as indicators of low dose radiation effects in clean-up workers of the Chernobyl nuclear reactor accident.
The goals of this study were to assess three biomarkers of genetic effect for their individual and collective ability to detect and estimate radiation exposure in Russian Chernobyl clean-up workers. Work assignments were planned to limit dose to 0.25 Gy. The three biomarkers employed were chromosome translocations detectcd in lynmphocytes by florescence in situ hybridisation (FISH), and mutation at two genes, glycophorin A (GPA) in red blood cells detected by flow cytometry and hypoxanthine phosphoribosyltransferase (HPRT) in lymphocytes detected by selective cell culture. Samples were Obtained from 1992 to 2000. The time between exposure at Chernobyl and sample acquisition was > or =5 years. The lymphocyte assays detected an elevation over controls in average outcomes it clean-up workers: translocation rates were 46% higher when adjusted for age and smoking and HPRT mutant frequencies were were 16% higher when adjusted for age. The G PA assay did not detect an exposure effect. The results indicate that measuring frequency of translocations by FISH is preferred for low dose radiation, retrospective biochemistry.
Jones
I M
IM
Biology and Biotechnology Research Program, L-441 Lawrence Livermore National Laboratory, Livermore, California, USA. jones20@llnl.gov
Tucker
J D
JD
Langlois
R G
RG
Mendelsohn
M L
ML
Pleshanov
P
P
Nelson
D O
DO
D000328:Adult; D004252:DNA Mutational Analysis; D004307:Dose-Response Relationship, Radiation; D005819:Genetic Markers; D006021:Glycophorins / Q000235:genetics*; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects*; D009154:Mutation / Q000528:radiation effects; D009784:Occupational Diseases / Q000175:diagnosis*; D016273:Occupational Exposure; D016133:Polymerase Chain Reaction; D011210:Power Plants; D011832:Radiation Injuries / Q000175:diagnosis* / Q000235:genetics; D018788:Radioactive Hazard Release; D014178:Translocation, Genetic; D014455:Ukraine
D005819:Genetic Markers; D006021:Glycophorins; D007041:Hypoxanthine Phosphoribosyltransferase