Gel-Phase synthesis of hydrophobic (thr14) (thr19) galanin (1 -19) fragment on a high capacity flexible crosslinked polystyrene support.
A hydrophobic analogue of human galanin (1-19) fragment has been synthesized using Boc/Bzl tactics to demonstrate the synthetic utility of the flexible crosslinked polystyrene support prepared by the suspension polymerization of styrene and 1,4-butanediol dimethacrylate. The copolymer was chloromethylated to 2.36 mmol Cl/g. The functionalized resin was found to possess all the physicochemical properties similar to Merrifield resin. The free peptide was obtained in high yield and purity as judged by RP-HPLC and characterized by amino acid analysis and ESI-MS.
Kumar
M K
MK
School of Chemical Sciences, Mahatma Gandhi University, Kottayam, India.
Mathe
B
B
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019004:Galanin / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D010446:Peptide Fragments; D011137:Polystyrenes; D019004:Galanin