Hepatitis A virus: a test method for virucidal activity.
Hepatitis A virus (HAV) is closely related to the genus enterovirus. HAV is very stable and resistant to acid pH and elevated temperature, as well as to chemicals and environmental influences. Human poliovirus is still one of the model viruses for testing disinfectants but there are discussions about changing to hepatitis A virus. The purpose of this study was to develop a method for using adapted hepatitis A virus to test hand disinfectants. Using HAV strains HM175/24a and FRhK-4 cytopathic effects were visible rarely, and not before 14 days. To verify virus growth in cells a RT-PCR was developed. Two disinfectants tested did not show the required virucidal activity to satisfy current German guidelines.
Wolff
M H
MH
Institute for Microbiology and Virology, University Witten/Herdecke, Germany. Whwolff@uni-wh.de
Schmitt
J
J
Rahaus
M
M
König
A
A
D019840:2-Propanol / Q000494:pharmacology*; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000494:pharmacology*; D017408:Guidelines as Topic; D006235:Hand Disinfection / Q000379:methods* / Q000592:standards; D030041:Hepatitis A virus / Q000145:classification / Q000187:drug effects* / Q000472:pathogenicity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008253:Macaca mulatta; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D014776:Virus Cultivation / Q000379:methods* / Q000592:standards
D000891:Anti-Infective Agents, Local; D004202:Disinfectants; D000431:Ethanol; D019840:2-Propanol