Overexpression of Pti5 in tomato potentiates pathogen-induced defense gene expression and enhances disease resistance to Pseudomonas syringae pv. tomato.
The tomato Pti5 gene encodes a pathogen-inducible ethylene response element-binding protein-like transcription factor that interacts with the disease resistance gene product Pto. Overexpression of Pti5 or Pti5-VP16, a translational fusion with a constitutive transcriptional activation domain, in tomato enhanced resistance to Pseudomonas syringae pv. tomato. Constitutive expression of Pti5 or Pti5-VP16 did not affect the basal level of pathogenesis-related gene expression, but it accelerated pathogen-induced expression of GluB and Catalase. The results demonstrate a positive role of Pti5 in defense gene regulation and disease resistance and suggest that a pathogen-activated posttranscriptional regulatory step is necessary for the pathogen induction of the defense gene expression.
He
P
P
Department of Plant Pathology, Kansas State University, Manhattan 66506, USA.
Warren
R F
RF
Zhao
T
T
Shan
L
L
Zhu
L
L
Tang
X
X
Zhou
J M
JM
D004268:DNA-Binding Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D011549:Pseudomonas / Q000472:pathogenicity*; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D010940:Plant Proteins; C106896:Pti5 protein, Lycopersicon esculentum; D014157:Transcription Factors