Biological activity of a mouse-human chimeric immunoglobulin G2 antibody to Cryptococcus neoformans polysaccharide.
The variable regions of the heavy and light chains of the protective murine monoclonal antibody (MAb) 2H1 (m2H1) were expressed with the human constant region genes for immunoglobulin G2 (IgG2) and kappa, respectively, to construct a chimeric antibody (ch2H1). ch2H1 retains the specificity of the parent MAb, exhibits biological activity, and lacks the toxicity of the parent murine IgG1 in chronically infected mice.
Westin Kwon
Katherine
K
Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, USA.
Lendvai
Nikoletta
N
Morrison
Sherie
S
Trinh
K Ryan
KR
Casadevall
Arturo
A
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003455:Cryptococcus neoformans / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology*; D051379:Mice; D011134:Polysaccharides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology*
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins