title: Relationship between sprouting axons, proteoglycans and glial cells following unilateral nigrostriatal axotomy in the adult rat.
abstract: Proteoglycans may modulate axon growth in the intact and injured adult mammalian CNS. Here we investigate the distribution and time course of deposition of a range of proteoglycans between 4 and 14 days following unilateral axotomy of the nigrostriatal tract in anaesthetised adult rats. Immunolabelling using a variety of antibodies was used to examine the response of heparan sulphate proteoglycans, chondroitin sulphate proteoglycans and keratan sulphate proteoglycans. We observed that many proteoglycans became abundant between 1 and 2 weeks post-axotomy. Heparan sulphate proteoglycans were predominantly found within the lesion core (populated by blood vessels, amoeboid macrophages and meningeal fibroblasts) whereas chondroitin sulphate proteoglycans and keratan sulphate proteoglycans were predominantly found in the lesion surround (populated by reactive astrocytes, activated microglia and adult precursor cells). Immunolabelling indicated that cut dopaminergic nigral axons sprouted prolifically within the lesion core but rarely grew into the lesion surround. We conclude that sprouting of cut dopaminergic nigral axons may be supported by heparan sulphate proteoglycans but restricted by chondroitin sulphate proteoglycans and keratan sulphate proteoglycans.
authors.affiliation: Physiological Laboratory, University of Cambridge, Downing Site, Tennis Court Road, Cambridge CB2 3EG, UK. lmoon@miamiproject.med.miami.edu
authors.forename: L D F
authors.initials: LD
authors.lastname: Moon
authors.forename: R A
authors.initials: RA
authors.lastname: Asher
authors.forename: K E
authors.initials: KE
authors.lastname: Rhodes
authors.forename: J W
authors.initials: JW
authors.lastname: Fawcett
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D019771:Axotomy; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020439:Growth Cones / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D007632:Keratan Sulfate / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D017072:Neostriatum / Q000254:growth & development / Q000293:injuries / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology*; D009434:Neural Pathways / Q000254:growth & development / Q000293:injuries / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013378:Substantia Nigra / Q000254:growth & development / Q000293:injuries / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D005904:Glial Fibrillary Acidic Protein; D019812:Heparan Sulfate Proteoglycans; D011509:Proteoglycans; D007632:Keratan Sulfate; D004298:Dopamine