[Detecting Epstein-Barr virus in nasopharyngeal carcinoma tissue using in situ polymerase chain reaction].
OBJECTIVE
To develop a sensitive non-isotopic method for in situ detection of Epstein-Barr virus (EBV) in paraffin-embedded section of nasopharyngeal carcinoma (NPC) tissue.


METHODS
The EBV BamHI-W fragment labeled with digoxigenin as probe was used to perform in situ hybridization and PCR in situ hybridization (in situ PCR).


RESULTS
The method of in situ PCR for the detection of EBV in NPC tissue was proved to be much more sensitive than in situ hybridization.


CONCLUSIONS
Hybridization in situ of PCR product is useful to detect EBV in NPC tissues.
Xu
L
L
Key Laboratory of Carcinogenesis of Chineses Ministry of Public Health, Hunan Medical University, Changsha, 410078, China.
Yang
X
X
Xie
L
L
D000230:Adenocarcinoma / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D009303:Nasopharyngeal Neoplasms / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods
D004279:DNA, Viral