[Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by AmpliSensor-PCR technique].
OBJECTIVE
To evaluate the clinical value of the quantitative detection of Mycobacterium tuberculosis DNA by Amplisensor-PCR in peripheral blood from patients with pulmonary tuberculosis.


METHODS
The model of Mycobacterium tuberculosis DNA in blood was made by QIAamp and AcuPure methods, quantitative detection of Mycobacterium tuberculosis DNA by AmpliSensor-PCR technique and the results were compared with IS6110 single-tube nested PCR (SN-PCR).


RESULTS
The peripheral blood from 200 patients with pulmonary tuberculosis were detected respectively by using AmpliSensor-PCR and IS6110 SN-PCR methods. The positive rates of Mycobacterium tuberculosis DNA were 60.5% and 63.5% respectively, and that from 85 patients with non-pulmonary tubeculosis were 4.7%, 8.2%.


CONCLUSIONS
AmpliSensor-PCR method showed higher sensitivity and specificity in detecting the Mycobacterium tubeculosis DNA from peripheral blood. The variation of the TB-DNA may be useful in evaluating the prognosis of the patients.
Tan
Y
Y
Pulmonary Disease Institute, Guangzhou Chest Hospital, Guangzhou 510095.
Li
Y
Y
Zhang
Y
Y
D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000382:microbiology*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic