[Influence of suppressor gene p16 on retinoic acid inducing lung cancer cell A549 differentiation].
OBJECTIVE
To investigate the role of suppressor gene p16 in the process of differential regulation of retinoic acid (RA) on the A549 lung cancer cells.


METHODS
Tumor suppressor gene p16 was transfered into A549 cells and the cells were treated with all-trans retinoic acid (ATRA) at the dosage of 5 x 10(-6) mol/L for 4 d. After that, the proliferation and differentiation of A549 cells were examined by growth curve and cytometry analysis, the change of lung lineage-specific marker MUC1 was tested by immunohistochemical staining. Meanwhile, Western blot was used to observe the change of p16 protein expression in A549 cells treated with ATRA.


RESULTS
ATRA could obviously inhibit the growth and induce the differentiation of A549 cells that were transfered with p16 gene. There were more cells arrested in G1/G0 phase and the expression of MUC1 was markedly down-regulated than in control cells. The expression of p16 protein was up-regulated in A549 cells treated with ATRA.


CONCLUSIONS
Suppressor gene p16 could enhance the effects of RA on proliferative suppression and differential induction of A549 cells.
Bai
M
M
Department of Pulmonary Medicine, Union Hospital of Tongji Medical College, Huazhong-University of Science and Technology, Wuhan 430022, China.
Zhang
X
X
Jin
Y
Y
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000187:drug effects* / Q000378:metabolism; D016193:G1 Phase; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D018396:Mucin-1 / Q000187:drug effects / Q000378:metabolism; D016192:Resting Phase, Cell Cycle; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018396:Mucin-1; D014212:Tretinoin