Dynamics and orientation of transmembrane peptide from bacteriorhodopsin incorporated into lipid bilayer as revealed by solid state (31)P and (13)C NMR spectroscopy.
13C and (31)P NMR spectra of a transmembrane peptide, [1-(13)C]Ala(14)-labeled A(6-34), of bacteriorhodopsin incorporated into dimyristoylphosphatidylcholine (DMPC) bilayer were recorded to clarify its dynamics and orientation in the lipid bilayer. This peptide is shown to take an alpha-helical form both in liquid crystalline and gel phases, as viewed from the conformation dependent (13)C chemical shifts. In addition, this peptide undergoes rapid rigid-body rotation about the helical axis at ambient temperature as viewed from the axially symmetric (13)C chemical shift anisotropy, whereas this symmetric anisotropy is changed to an asymmetric pattern at temperatures below 10 degrees C. We further incorporated the peptide into the spontaneously aligned DMPC bilayer to applied magnetic field, induced by dynorphin (dynorphin:DMPC =1:10), a heptadeca-opioid peptide with very high affinity to opioid receptor, in order to gain insight into its orientation in the bilayer. This magnetically aligned system turned out to be persistent even at 0 degrees C as viewed from (31)P NMR spectra of the lipid bilayer, after this peptide was incorporated into this system [A(6-34): dynorphin: DMPC = 4:10:100]. It was found from the (13)C NMR spectra of [1-(13)C]Ala(14) A(6-34) that the helical axis of A(6-34) is oriented parallel to the bilayer normal irrespective of the presence or absence of reorientation motion about the helical axis at a temperature above the lowered gel to liquid crystalline phase transition.
Kimura
Shigeki
S
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, 3-2-1 Kouto, Kamigori, Hyogo 678-1297, Japan.
Naito
Akira
A
Tuzi
Satoru
S
Saitô
Hazime
H
D000595:Amino Acid Sequence; D016880:Anisotropy; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D002247:Carbon Isotopes; D003198:Computer Simulation; D004399:Dynorphins / Q000737:chemistry; D006217:Halobacterium; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry; D010759:Phosphorus Isotopes; D017433:Protein Structure, Secondary; D012399:Rotation; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014961:X-Ray Diffraction
D002247:Carbon Isotopes; D008051:Lipid Bilayers; D010446:Peptide Fragments; D010759:Phosphorus Isotopes; D001436:Bacteriorhodopsins; D004399:Dynorphins