title: [Osteogenetic activity of BMP-3-coated titanium specimens of different surface texture at the orthotopic implant bed of giant rabbits].
abstract: BACKGROUND
  The successful combination of osteoinductive factors with current materials used in both endoprosthetics and implantology improves bony ingrowth and long-term stability of the chosen implants. The aim of the present experimental animal study was to clarify in what way faster bony integration can be achieved through additional BMP-3-coating of titanium test implants of different surface textures (hydroxy-apatite-coated or corundum-blasted).
  
  
  METHODS
  Thirty of 60 cylindrical titanium test implants with a hydroxy-apatite or corundum-blasted surface were coated with 230 microg porcine, high-purified BMP-3-precipitate per implant to check their osteoinductive potential in a bioassay. In each case a BMP-3-coated and an uncoated control-device were implanted with a gap formation of 1 mm into the femoral part of the patellofemoral joint of the right and left leg of 30 adult giant rabbits. Serial saw slices of all explanted specimens were prepared, and the osseous integration of the implant and time-dependent bone neoformation were analyzed microscopically and histomorphometrically 14, 35, and 56 days after implantation.
  
  
  RESULTS
  Coating of TiAl4V6-test devices with BMP-3 led in both groups after gap implantation to an improved osseointegration, that was histomorphological and histomorphometrical verifiable. Statistical evaluation using the t-test for matched samples showed 5 weeks after surgery a significant higher volume of new formed bone of the BMP-3-coated corundum-blasted or hydroxy-apatite-coated TiAl4V6 test devices compared to the non-coated controls of the same type (P < 0.01). Light microscopy demonstrated osseointegration without connective tissue membrane around the surface of the implants after 2, 5, and 8 weeks. Better osseointegration was achieved in the hydroxy-apatite-coated implants than in the corundum-blasted implants.
  
  
  CONCLUSIONS
  Our results indicate that composite metal implants, as used in endoprosthetics and implantology, are suitable carriers for BMP-3 and improved fixation of the implants can be achieved.
authors.affiliation: Chirurgische Klinik mit Poliklinik, Berufsgenossenschaftliche Kliniken Bergmannsheil, Universitätsklinik der Ruhr-Universität, Bochum. Stefan.A.Esenwein@ruhr-uni-bochum.de
authors.forename: S A
authors.initials: SA
authors.lastname: Esenwein
authors.forename: S
authors.initials: S
authors.lastname: Esenwein
authors.forename: G
authors.initials: G
authors.lastname: Herr
authors.forename: G
authors.initials: G
authors.lastname: Muhr
authors.forename: W
authors.initials: W
authors.lastname: Küsswetter
authors.forename: C H
authors.initials: CH
authors.lastname: Hartwig
mesh_terms: D000497:Alloys; D000818:Animals; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible; D005269:Femur / Q000473:pathology / Q000601:surgery; D016348:Osseointegration / Q000187:drug effects*; D019736:Prostheses and Implants; D011817:Rabbits; D013499:Surface Properties; D013552:Swine; D014025:Titanium
chemical_list: D000497:Alloys; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D014025:Titanium
vernacular_title: Osteogene Aktivität BMP-3-besichichteter Titanprüfkörper unterschiedlicher Oberflächenbeschaffenheit im orthotopen Lagen des Riesenkaninchens.