pasilla, the Drosophila homologue of the human Nova-1 and Nova-2 proteins, is required for normal secretion in the salivary gland.
From a screen for genes expressed and required in the Drosophila salivary gland, we identified pasilla (ps), which encodes a set of proteins most similar to human Nova-1 and Nova-2. Nova-1 and Nova-2 are nuclear RNA-binding proteins normally expressed in the CNS where they directly regulate splicing. In patients suffering from paraneoplastic opsoclonus myoclonus ataxia (POMA), Nova-1 and Nova-2 proteins are present as auto-antigens. Consistent with a role in splicing, PS is localized to nuclear puncta. The salivary glands of ps mutants internalize normally and maintain epithelial polarity. However, the mutant salivary glands develop irregularities in overall morphology and have defects in apical secretion. The secretory defects in ps mutants provide a potential mechanism for the loss of motor function observed in POMA patients.
Seshaiah
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Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, Maryland 21205-2196, USA.
Miller
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Myat
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Andrew
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DJ
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D001324:Autoantigens; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004330:Drosophila; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology*; D015723:Gene Library; D005816:Genetic Complementation Test; D006801:Humans; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology*; D012469:Salivary Glands / Q000196:embryology* / Q000378:metabolism* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
D000951:Antigens, Neoplasm; D001324:Autoantigens; D029721:Drosophila Proteins; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C445001:ps protein, Drosophila