[The effect of immune bovine whey on cell-associated glucosyltransferase activity of Streptococcus mutans].
OBJECTIVE
To study the effect of immune bovine whey on cell-associated glucosyltransferase (GTF) activity of S. mutans MT8148.


METHODS
The immune milk was collected from cows immunized with cell-associated GTF overexpression strain B-29-33 of S. mutans MT8148. The control milk was from non-immunized cows. The immune absorbed whey was gotten from immune bovine whey which was absorbed with lyophilized Formalin-killed B-29 whole cells. Three kinds of whey were subdivided into three groups: 50 microliters, 70 microliters, 90 microliters. The content of insoluble glucan was estimated colorimetrically by anthrone method.


RESULTS
The control bovine whey had an enhancing GTF activity (407.00%-485.62%). The immune absorbed whey inhibited partly the enhancing GTF activity (208.74%-273.00%). The immune whey inhibited significantly the GTF activity (70.24%-38.62%) and the inhibition showed a tendency to depend on doses.


CONCLUSIONS
The immune bovine whey inhibits significantly the cell-associated GTF activity of S. mutans MT8148.
Chen
W
W
Department of Operative Dentistry, Cariology and Endodontology, School of Stomatology, Hubei Medical University, Wuhan 430079, China.
Fan
M
M
Bian
Z
Z
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002417:Cattle; D005964:Glucosyltransferases / Q000276:immunology / Q000378:metabolism*; D007114:Immunization; D008892:Milk / Q000276:immunology*; D013295:Streptococcus mutans / Q000201:enzymology*
D000906:Antibodies; D005964:Glucosyltransferases