[Phase-specific elements of the regulatory zone of the Drosophila melanogaster string gene].
The phases of the reporter gene expression controlled by different fragments of the string (stg) gene regulatory region were determined in Drosophila neuroblasts by detection of beta-galactosidase activity and radioautography. In the D10 and D22 lines carrying the constructs pstg beta-E4.9 and pstg beta-E5.3, respectively, the reporter gene activity was detected in the G1 phase of the cell cycle. In the D12 and D20 lines (pstg beta-E6.4 and pstg beta-E2.6), no periodic expression was observed. The regulatory regions of the stg from lines D10 and D22 and that of Drosophila gene cyclin D shared consensus aagaactttg, which was also expressed in the G1 phase. The phase-specific expression of the cell-cycle genes was compared in a model for the mitotic-wave cells of eye imaginal disk and neuroblasts of the nerve ganglia.
Trunova
S A
SA
Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, 630090 Russia.
Dubatolova
T D
TD
Omel'ianchuk
L V
LV
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002843:Chromatin / Q000235:genetics; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D001616:beta-Galactosidase / Q000235:genetics
D018797:Cell Cycle Proteins; D002843:Chromatin; D029721:Drosophila Proteins; D004247:DNA; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; C059217:stg protein, Drosophila; D001616:beta-Galactosidase
Fazospetsifichnye élementy reguliatornoĭ zony gena string Drosophila melanogaster.