Selective liquid chromatographic method for determination of fluoxetine in plasma.
A selective and sensitive liquid chromatographic method was developed for the determination of fluoxetine (FLU) in plasma. FLU was isolated from plasma by liquid-liquid extraction. The chromatographic separation was performed on an analytical 250 x 3.9 mm id Novapak C18 column (4 microm particle size) with an isocratic mobile phase consisting of phosphate buffer-acetonitrile-methanol -triethylamine (58 + 30 + 10 + 2, v/v) adjusted to pH 7. Using UV detection at 226 nm, the detection limit for FLU in plasma was 3 ng/mL. No interferences were found with tricyclic antidepressant drugs, which allows this method to be used in clinical studies. The calibration curve was linear over the concentration range of 10-200 ng/mL. The average recovery was about 80% for plasma. The inter- and intraday assay coefficients of variation were <8%.
Zarghi
A
A
Shaheed Beheshti University of Medical Sciences, Department of Pharmacy, Tehran, Iran. azarghi@safineh.net
Kebriaeezadeh
A
A
Ahmadkhaniha
R
R
Akhgari
M
M
Rastkari
N
N
D018687:Antidepressive Agents, Second-Generation / Q000097:blood* / Q000592:standards; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D005473:Fluoxetine / Q000097:blood* / Q000592:standards; D006801:Humans; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
D018687:Antidepressive Agents, Second-Generation; D005473:Fluoxetine