The crystal structure and mechanism of 1-L-myo-inositol- 1-phosphate synthase.
1-l-myo-Inositol-1-phosphate synthase catalyzes the conversion of d-glucose 6-phosphate to 1-l-myo-inositol-1-phosphate (MIP), the first and rate-limiting step in the biosynthesis of all inositol-containing compounds. It involves an oxidation, intramolecular aldol cyclization, and reduction. We have determined the first crystal structure of MIP synthase. We present structures of both the NAD-bound enzyme and the enzyme bound to an inhibitor, 2-deoxy-glucitol-6-phosphate. While 58 amino acids are disordered in the unbound form of the enzyme in the vicinity of the active site, the inhibitor nucleates the folding of this domain in a striking example of induced fit, serving to completely encapsulate it within the enzyme. Three helices and a long beta-strand are formed in this process. We postulate a mechanism for the conversion based on the structure of the inhibitor-bound complex.
Stein
Adam J
AJ
Department of Chemistry, Michigan State University, East Lansing, Michigan 48824, USA.
Geiger
James H
JH
D003460:Crystallization; D003461:Crystallography; D007296:Myo-Inositol-1-Phosphate Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D011487:Protein Conformation; D013012:Sorbitol / Q000031:analogs & derivatives* / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
C403168:2-deoxyglucitol 6-phosphate; D013403:Sugar Phosphates; D009243:NAD; D013012:Sorbitol; D007296:Myo-Inositol-1-Phosphate Synthase