title: A serodiagnostic ELISA using recombinant antigen of swine transmissible gastroenteritis virus nucleoprotein.
abstract: A serodiagnostic ELISA utilizing the recombinant nucleoprotein (rN protein) of transmissible gastroenteritis virus (TGEV) was developed, and evaluated by examining a panel of 141 virus neutralization (VN) positive and 101 negative sera. The rN protein-based ELISA (rnELISA) appeared to be highly sensitive and specific (98.6% and 98.0%, respectively) when it was compared to the VN test. The result was similar to that of an ELISA based on purified viral antigens with showing good correlation (R=0.829). No cross-reaction was detected with antisera against porcine epidemic diarrhea virus, hog cholera virus, type A rotavirus, pseudorabies virus and swine vesicular disease virus in this ELISA. The rnELISA can be an alternative for the diagnosis of TGE with a great advantage in antigen preparation.
authors.affiliation: Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Kokuho
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Inumaru
authors.forename: Y
authors.initials: Y
authors.lastname: Yokomizo
authors.forename: T
authors.initials: T
authors.lastname: Onodera
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis* / Q000821:virology; D008969:Molecular Sequence Data; D009698:Nucleoproteins; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D018076:DNA, Complementary; D009698:Nucleoproteins; D012367:RNA, Viral; D011994:Recombinant Proteins