[In vitro and in vivo experiment of transduction of reporter gene into tumors by E5 gene delivery system].
OBJECTIVE
To investigate the spectrum of tumors into which the E5 gene delivery system can transduce reporter gene so as to establish a platform technology that one gene delivery system can be used to treat multiple types of tumors.


METHODS
The E5 delecvery system was used to transduce reporter genes into various types of IGF I R positive tumor cell lines in vitro and into different IGF I R positive tumors transplanted subcutaneously in nude mice in vivo. The efficiency of trasduction was examined.


RESULTS
The E5 delivery system transferred reporter genes into five types of tumor cells that over-express IGFIR in vitro and transferred reporter genes into different tumors transplanted subcutaneously in nude mice that over-express IGFIR in vivo too. The transduction rate was positively correlated with the expression rate of IGFIR. However, the E5 delivery system failed to transfer reporter genes into tumor cedll lines in vitro and the tumors transplanted subcutaneously in nude mice in vivo, both of which were without IGF I R expression.


CONCLUSIONS
E5 delivery system has rather remarkable target-ability and extensive usefulness. It has the potentiality in gene therapy of cancer.
Ren
C
C
Shanghai Cancer Institute, Shanghai 200032, China.
Li
J
J
Zhao
R
R
D000818:Animals; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics / Q000628:therapy; D015513:Oncogene Proteins / Q000235:genetics*; D017451:Receptors, Somatomedin / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods; D014407:Tumor Cells, Cultured; D014764:Viral Proteins
C116205:E5 protein, cottontail rabbit papillomavirus; D015513:Oncogene Proteins; D017451:Receptors, Somatomedin; D014764:Viral Proteins