title: [EBV latent membrane protein 1 induces p53 expression via NF-kappa B in nasopharyngeal carcinoma].
abstract: OBJECTIVE
  To ascertain if EBV-encoded latent membrane protein 1 (LMP1) induces p53 expression via NF-kappa B signaling.
  
  
  METHODS
  A nasopharyngeal carcinoma cell line, Tet-on-LMP1 HNE2, transfected with LMP1, the expression of which was regulated by tetracycline, was used in this study. Functional activity of NF-kappa B was determined by luciferase reporter assay and expression of p53 and bcl-2 was detected by Western blot.
  
  
  RESULTS
  LMP1 induced p53 expression via NF-kappa B signaling pathway. Induction of p53 expression could be blocked by phosphorothiate analogs of antisense oligonucleotides to NF-kappa B p65 and LMP1, but not by NF-kappa B p50. However, it seemed that LMP1 had no influence on bcl-2 expression in nasopharyngeal carcinoma.
  
  
  CONCLUSIONS
  Induction expression of p53 by EBV-encoded LMP1 implies that p53 may act as a mediator in apoptosis triggered by LMP1, which brings about a complex balance in nasopharyngeal carcinogenesis.
authors.affiliation: Cancer Research Institute, Hunan Medical University, Changsha, Hunan 410078, China.
authors.forename: W
authors.initials: W
authors.lastname: Liao
authors.forename: M
authors.initials: M
authors.lastname: Tang
authors.forename: L
authors.initials: L
authors.lastname: Yin
mesh_terms: D004854:Herpesvirus 4, Human / Q000737:chemistry*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology*; D052002:NF-kappa B p50 Subunit; D009303:Nasopharyngeal Neoplasms; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D015398:Signal Transduction; D051996:Transcription Factor RelA; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000494:pharmacology*
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D051996:Transcription Factor RelA; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins