[Experimental study on prefabricating iliac grafts using composite bone].
OBJECTIVE
To investigate the feasibility of prefabricating a specified shape autograft capable of transfer using coral and type I collagen as a carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2).


METHODS
In this study, the composite of rhBMP-2, coral and type I collagen was made certain shape to prefabricate vascularized osteomuscular autograft capable of microvascular free tissue transfer and autogenous bone graft with certain shape and titanium implant in it. The composite was implanted in the iliac area in dog with the titanium implant at the same time. After 3 months and 4 and a half months of implantation, the composites were studied with gross measurement, X-ray, and histological examinations.


RESULTS
After 3 months, composited bone was turned to bone tissue, and the shape of iliac bone was changed with implant in it, bone interface was seen between new bone and implant. And new bone was matured after 4 and a half months.


CONCLUSIONS
Coral and type I collagen are effective carrier for rhBMP-2 to prefabricate vascular osteomuscular autograft with certain shape. The use of rhBMP-2 for tissue engineered microvascular free bone flaps has an unlimited potential and adds a new dimension to maxillofacial reconstruction.
Ma
Q
Q
College of Stomatology, Fourth Military Medical University, Xi'an Shanxi, P. R. China 710032. maqinsoh@pub.xaonline.com
Mao
T
T
Liu
B
B
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000627:therapeutic use*; D024042:Collagen Type I / Q000627:therapeutic use; D004285:Dogs; D007085:Ilium / Q000601:surgery*; D008297:Male; D019736:Prostheses and Implants; D011994:Recombinant Proteins / Q000627:therapeutic use; D023822:Tissue Engineering; D014025:Titanium; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D024042:Collagen Type I; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014025:Titanium