Infrequent p16/CDKN2 alterations in squamous cell carcinoma of the oesophagus.
Loss of heterozygosity (LOH) on chromosome 9 and p16 (MTS1/CDKN2) gene mutations have been reported in various human cancers. The present study aimed to determine the prevalence of LOH in 100 oesophageal squamous cell carcinomas (OSCCs) by typing microsatellite loci and mutations of the p16 gene. The methods used included denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of exon 2. LOH was found in 14.7% of the OSCC cases. Six gene alterations were identified in exon 2. They consisted of three deletions and the same polymorphism in three samples. The relatively low rate of p16 mutation compared with the frequency of LOH suggests the possible involvement of another tumour suppressor gene located on chromosome 9 in oesophageal carcinogenesis.
Giroux
Marie-Agnès
MA
Service d'Hépatogastroentérologie, Hôpital de La Cavale Blanche, Brest, France.
Audrezet
Marie-Pierre
MP
Metges
Jean-Philippe
JP
Lozac'h
Patrick
P
Volant
Alain
A
Nousbaum
Jean-Baptiste
JB
Labat
Jean-Paul
JP
Gouérou
Hervé
H
Ferec
Claude
C
Robaszkiewicz
Michel
M
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9; D004591:Electrophoresis, Polyacrylamide Gel; D004938:Esophageal Neoplasms / Q000235:genetics*; D005260:Female; D019942:Genes, p16; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction