title: Growth and histamine formation of Morganella morganii in determining the safety and quality of inoculated and uninoculated bluefish (Pomatomus saltatrix).
abstract: The objective of this study was to determine the effect of normal microflora and Morganella morganii on histamine formation and olfactory acceptability in raw bluefish under controlled storage conditions. Fillets inoculated with and without M. morganii were stored at 5, 10, and 15 degrees C for 7 days. Microbial isolates from surface swabs were identified and screened for histidine decarboxylase activity. Olfactory acceptance was performed by an informal sensory panel. Histamine levels were quantified using high-performance liquid chromatography and fluorescence detection. While olfactory acceptance decreased, histamine concentration and bacterial counts increased. Storage temperature had a significant effect on histamine levels, bacterial counts, and olfactory acceptance of the bluefish. Inoculation with M. morganii had a positive significant effect on histamine formation for bluefish held at 10 and 15 degrees C (P < 0.0001). The results of the study will serve in supporting U.S. Food and Drug Administration (FDA) regulations regarding guidance and hazard levels of histamine in fresh bluefish.
authors.affiliation: Department of Food Science and Technology, Virginia Tech, Blacksburg 24061-0418, USA.
authors.forename: T A
authors.initials: TA
authors.lastname: Lorca
authors.forename: T M
authors.initials: TM
authors.lastname: Gingerich
authors.forename: M D
authors.initials: MD
authors.lastname: Pierson
authors.forename: G J
authors.initials: GJ
authors.lastname: Flick
authors.forename: C R
authors.initials: CR
authors.lastname: Hackney
authors.forename: S S
authors.initials: SS
authors.lastname: Sumner
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015169:Colony Count, Microbial; D005399:Fishes / Q000382:microbiology*; D005453:Fluorescence; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000378:metabolism*; D020613:Morganella morganii / Q000254:growth & development* / Q000378:metabolism; D009812:Odorants; D011786:Quality Control; D012449:Safety; D013696:Temperature; D013997:Time Factors; D014481:United States; D014486:United States Food and Drug Administration
chemical_list: D006632:Histamine; D006640:Histidine Decarboxylase