title: Feline immunodeficiency virus Gag- and Env-specific immune responses after vaginal versus intravenous infection.
abstract: To better understand the correlation of mucosal and systemic immune responses with lentiviral containment, we contrasted the early mucosal and systemic immune responses induced by vaginal versus intravenous exposure of cats to feline immunodeficiency virus (FIV) isolates of differing pathogenicity and clade (i.e., FIV-B-2542 and FIV-A-PPR). We found that despite divergence in viral genotype, the mucosal and systemic immune responses induced differed more with route of exposure than virus isolate. In intravenously exposed cats, Gag-specific antibody (both IgG and IgA isotype) predominated in the serum, saliva, and vaginal wash fluid irrespective of infecting virus isolate. While Env-specific responses were more variable, they were more often detected in vaginally infected cats. Both IgG and IgA directed against Gag and Env were consistently present in vaginal wash fluids independent of route of infection or virus isolate. FIV Gag- and Env-specific cytotoxic lymphocytes (CTLs) were detected in blood and tissue lymphocytes of cats infected with either virus strain but were greatest in intravenously infected animals. Likewise, FIV-specific CTLs were detected in CD8(+) vaginal lymphocytes of animals infected by either route but were also more frequent in intravenously inoculated animals. In summary, we found qualitative differences in the immune responses following vaginal infection but no evidence (1) that mucosal immune responses were enhanced in vaginally exposed cats, (2) that local mucosal infection led to measurably greater immune responses in either compartment; or (3) that more prominent immune responses correlated with lower viral burden.
authors.affiliation: Department of Microbiology, Pathology, and Parasitology, North Carolina State University, Raleigh, North Carolina 27606, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Burkhard
authors.forename: C K
authors.initials: CK
authors.lastname: Mathiason
authors.forename: T
authors.initials: T
authors.lastname: Bowdre
authors.forename: E A
authors.initials: EA
authors.lastname: Hoover
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000635:transmission*; D005260:Female; D005434:Flow Cytometry; D015683:Gene Products, gag / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014621:Vagina / Q000276:immunology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral; D015683:Gene Products, gag; D006023:Glycoproteins; D014759:Viral Envelope Proteins; C445079:envelope protein, Feline immunodeficiency virus; C074662:p24 protein, Feline immunodeficiency virus