title: Safely reducing manual urine microscopy analyses by combining urine flow cytometer and strip results.
abstract: We aimed to reduce the number of manual urine microscopy examinations safely by cross-interpretation of the Sysmex UF-100 (TOA Medical Electronics, Kobe, Japan) and urine strip results such that microscopy would be performed if there was discordance between the UF-100 and urine strip results. We also evaluated the usefulness of the optional UF-100 expert software. We performed 2 studies: study 1 to establish review rules for eventual microscopic examination; study 2, a validation study. Our review rates were 40% and 48% and those of UF-100 software were 16% and 32% for the 2 studies. Our false-positive and false-negative results, among the samples not flagged for microscopic review, were acceptably low. We did not find a good correlation between the microscopic classification of RBC morphologic features and the classification given by the UF-100. Since incorporation of the automated urine strip reader and the UF-100 in routine use, our manual microscopy has been reduced to less than 40%.
authors.affiliation: Department of Laboratory Medicine, University Hospital Leuven, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Roggeman
authors.forename: Z
authors.initials: Z
authors.lastname: Zaman
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005103:Expert Systems; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry / Q000379:methods* / Q000592:standards; D006801:Humans; D019044:Image Cytometry / Q000379:methods / Q000592:standards; D007223:Infant; D008297:Male; D008853:Microscopy / Q000379:methods* / Q000592:standards; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve; D011934:Reagent Strips / Q000592:standards; D015203:Reproducibility of Results; D016482:Urinalysis / Q000379:methods* / Q000592:standards
chemical_list: D011934:Reagent Strips