Isolation of MUC 1 antigen from human gastric juice.
Episialin (MUC 1 antigen) is a membrane bound mucin. It is located on the surface of the epithelium. It can also be detected in soluble form in body fluids. In our paper we present method of the purification of this antigen from human gastric juice. MUC 1 antigen was isolated from human gastric juice by the gel filtration on Sepharose 4B column and affinity chromatography with HMFG-1 antibody coupled to CNBr activated Sepharose 4B. Using presented methods for purification of MUC 1 antigen from gastric juice we received after immunostaining one diffuse band of about 400 kDa molecular weight. Our results revealed that presented method of isolation of MUC 1 antigen from gastric juice is a valuable and sufficient procedure for purification of soluble form of this antigen.
Minkiewicz-Radziejewska
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I
Department of General and Organic Chemistry, Medical Academy of Białystok, Poland.
Gindzieński
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A
Namiot
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Z
D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D005750:Gastric Juice / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D018396:Mucin-1 / Q000737:chemistry / Q000302:isolation & purification*
D018396:Mucin-1