Nuclear estrogen receptor II (nER-II) is involved in the estrogen-dependent ribonucleoprotein transport in the goat uterus: II. Isolation and characterization of three small nuclear ribonucleoprotein proteins which bind to nER-II.
Three proteins of a goat uterine small nuclear ribonucleoprotein (snRNP) fraction, which bind to nuclear estrogen receptor-II (nER-II) have been isolated and purified. These are the p32, p55, and p60 of which p32 is the major nER-II binding protein. Indirect evidence reveals that p32 binds to the nuclear export signal (NES) on the nER-II. nER-II is a snRNA binding protein while p32 does not bind to the RNA. nER-II along with p32 and p55 form an effective Mg(++)ATPase complex, the activation of which appears to be the immediate reason behind the RNP exit from the nuclei following estradiol exposure. The three nER-II binding proteins bind to the nuclear pore complex; nER-II does not possess this property.
Sebastian
Thomas
T
Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram-695014, Kerala, India.
Thampan
Raghava Varman
RV
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006041:Goats; D011485:Protein Binding; D021381:Protein Transport; D011960:Receptors, Estrogen / Q000378:metabolism / Q000502:physiology*; D017411:Ribonucleoproteins, Small Nuclear / Q000302:isolation & purification* / Q000378:metabolism
D011960:Receptors, Estrogen; D017411:Ribonucleoproteins, Small Nuclear; D000251:Adenosine Triphosphatases