[Effect of tongbiling on synoviocyte function in adjuvant arthritis rats].
OBJECTIVE
To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha) and postaglandin E2(PGE2) secretion by synoviocytes in adjuvant arthritis (AA) rats.


METHODS
Synovial fibroblasts was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL-1, TNF-alpha bioactivity and PGE2 content of supernatants of cultured synoviocytes were measured.


RESULTS
TBL could inhibit significantly the synovial fibroblast proliferation (P < 0.001), and down-regulated IL-1, TNF-alpha and PGE2 productions (P < 0.01); indomethacin could promote obviously the synovial fibroblast proliferation (P < 0.001). It inhibited significantly PGE2 production, but further up-regulated the IL-1 and TNF-alpha secretion by synoviocytes (P < 0.01).


CONCLUSIONS
The therapeutical effect of TBL on AA might be associated with its down-regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels.
Chen
J
J
First Affiliated Hospital, Guangzhou TCM University, Guangzhou (510405).
Zhao
H
H
Shen
X
X
D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D007375:Interleukin-1 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone