Immunocytochemical localization of antigens recognized by asymptomatic microfilaremic patient's antisera in microfilariae of Wuchereria bancrofti.
Ultrathin sections of microfilariae of Wuchereria bancrofti embedded in hydrophilic resin were incubated with sera from patients, using antisera from asymptomatic microfilaremic patients with different microfilarial densities [1-100 microfilariae (mf)/ml, 101-500 mf/ml, > 1,000 mf/ml]. All groups studied showed reactivity against relevant epitopes in all tissues of microfilariae of W. bancrofti, instead of being localized in a specific nematode region, although the number of colloidal per square micron was inversely proportional to the microfilaremia. Such results confirm data obtained by other authors and indicate a possible role for the humoral response in the mechanism for the destruction of circulating microfilariae.
Alves
L C
LC
Departamento de Patologia e Biologia Celular, Centro de Pesquisas Aggeu Magalhães, Universidade Federal de Pernambuco, Recife, CEP, Brazil.
Brayner
F A
FA
Silva
L F
LF
Miranda
J
J
Rocha
A
A
Peixoto
C A
CA
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000032:analysis*; D004605:Elephantiasis, Filarial / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008842:Microfilariae / Q000276:immunology; D014958:Wuchereria bancrofti / Q000254:growth & development / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth