title: Retinal ganglion cell death after acute retinal ischemia is an ongoing process whose severity and duration depends on the duration of the insult.
abstract: In adult Sprague-Dawley rats we have investigated retinal ganglion cell survival after transient intervals of retinal ischemia of 30, 45, 60, 90 or 120 min duration, induced by ligature of the ophthalmic vessels. Animals were killed 5, 7, 14, 21, 30, 60, 90 or 180 days later and densities of surviving retinal ganglion cells were estimated in retinal whole mounts by counting cells labelled with diAsp. This dye was applied, 3 days prior to death, to the ocular stump of the intraorbitally transected optic nerve. We found that retinal ganglion cell loss after retinal ischemia proceeds for different lengths of time. All the ischemic intervals induced loss of retinal ganglion cells whose severity and duration was related to the length of the ischemic interval. Following 30 or 45 min of ischemia, cell loss lasted 14 days and caused the death of 46 or 50%, respectively, of the population of retinal ganglion cells. Sixty, 90 or 120 min of retinal ischemia were followed by a period of cell loss that lasted up to 90 days and caused the death of 75%, 87% or 99%, respectively, of the population of retinal ganglion cells. We conclude that retinal ganglion cell loss after retinal ischemia is an ongoing process that may last up to 3 months after the injury and that its severity and duration are determined by the ischemic interval.
authors.affiliation: Laboratorio de Oftalmología Experimental, Departamento de Oftalmología, Facultad de Medicina, Universidad de Murcia, E-30.100 Espinardo, Murcia, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Lafuente
authors.forename: M P
authors.initials: MP
authors.lastname: Villegas-Pérez
authors.forename: I
authors.initials: I
authors.lastname: Sellés-Navarro
authors.forename: S
authors.initials: S
authors.lastname: Mayor-Torroglosa
authors.forename: J
authors.initials: J
authors.lastname: Miralles de Imperial
authors.forename: M
authors.initials: M
authors.lastname: Vidal-Sanz
mesh_terms: D000208:Acute Disease; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology*; D002452:Cell Count; D016923:Cell Death / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D018450:Disease Progression; D005456:Fluorescent Dyes; D009410:Nerve Degeneration / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D011726:Pyridinium Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology; D012164:Retinal Diseases / Q000473:pathology / Q000503:physiopathology*; D012165:Retinal Ganglion Cells / Q000473:pathology*; D013266:Stilbamidines; D013997:Time Factors
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D013266:Stilbamidines; C076868:4-(4-dihexadecylaminostyryl)-N-methylpyridium