[The role of TGF-beta on Tca83--a tongue squamous cell carcinoma cell line].
OBJECTIVE
To investigate the role of TGF-beta on the growth of Tca83 cells.


METHODS
The cell culture, MTT detection, immunohistochemistry and in situ hybridization detection methods were used.


RESULTS
After 96 h, compared with the control, most groups with low concentrations of TGF-beta (0.2, 1.0, 5.0, 12.5 micrograms/L) showed growth inhibition, while the group with high concentration of TGF-beta (25.0 micrograms/L) showed no growth inhibition. Positive stain of TGF-beta 1, T beta R I mRNA and TGF-beta 1, T beta R I, T beta R II protein could be detected in Tca83 cells.


CONCLUSIONS
Tca83 cell line has the potentiality to secret TGF-beta 1. Autocrine and paracrine of TGF-beta 1 could regulate cancer cell's proliferation and differentiation directly. Since the ligand-receptor conduction in Tca83 cell line is intact, external TGF-beta 1 could combine with the T beta R I and T beta R II receptor and exert its inhibitory effect on Tca83 cell line.
Liu
X
X
Department of Oral Pathology, Peking University School of Stomatology, Beijing 100081, China.
Gao
Y
Y
D030201:Activin Receptors, Type I / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D014062:Tongue Neoplasms / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I