[Use of molecular biological method (MAPREC-test) for evaluating residual neurovirulence and genetic stability of oral poliovirus vaccine type 3].
MAPREC test (mutant analysis by polymerase chain reaction and restriction enzyme cleavage), evaluating the content of neurovirulent mutants with nucleotide substitution of U for C in position 472 in the 5'-nontranslated RNA region, in a virus population was used for a retrospective analysis of 3 specimens of inoculated viruses and 55 lots of type 3 oral poliovirus vaccine (OPV) prepared at M. P. Chumakov Institute of Poliomyelitis, Russian Academy of Medical Sciences, during recent 6 years. The mean content of neurovirulent mutants in inoculated viruses was 0.26 +/- 0.06%, in monovaccines 0.34 +/- 0.04% (the maximum allowable content is 1%). The method is well reproducible and detects about 0.1% mutants in the population. MAPREC test can be used instead of currently used residual virulence control on monkeys, as it detects viral preparations which were not tested on monkeys and does not reject high-quality vaccines. The protocol of using this method in combination with other methods for neurovirulence and genetic stability control of type 3 OPV is discussed.
Rumiantsev
A A
AA
Grachev
V P
VP
Kozlov
V G
VG
Karganova
G G
GG
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral; D005069:Evaluation Studies as Topic; D000882:Haplorhini; D009420:Nervous System / Q000821:virology*; D017955:Poliovirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D014774:Virulence
D020121:5' Untranslated Regions; D017931:DNA Primers; D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral
Ispol'zovanie molekuliarno-biologicheskogo metoda (MAPREC-testa) dlia otsenki ostatochnoĭ neĭrovirulentnosti i geneticheskoĭ stabil'nosti oral'noĭ poliovirusnoĭ vaktsiny tipa 3.