Embryotoxicity of 1,2-dibromoethane in chick embryos in ovo: early and late effects.
Embryotoxic effects of 1,2-dibromoethane (DBE), a compound still widely used in industry, have been analyzed using chick embryos in ovo. Administration on embryonic days (ED) 3,4 or 5 induced dose-dependent embryotoxicity, manifested namely as the early embryonic death. A serious disturbance of the vascular system represented probably the main cause of strong embryolethality and growth retardation in the group of survivors. Amniotic bands in the parietal region and defects of brain and aorta prevailed in the malformation spectrum registered on ED 10. The local character of early induced changes suggests a direct effect of DBE itself in the embryotoxic action. This process is probably accomplished through interaction with lipids in cell membranes owing to the hydrophobic character of DBE molecules. The results, however, did not exclude an involvement of reactive metabolites in final embryotoxicity via the formation of DNA-adducts. In any case, a decreasing embryotoxicity of DBE with the age of treated embryos documented that the onset of liver function, assumed to occur on ED 5, did not increase the efficacy of DBE bioactivation. Our results confirmed the short-term embryotoxic properties of DBE reported in rat embryonic cultures. In addition, the in ovo system enabled us to reveal also long-term consequences represented namely by the formation of amniotic bands, not detectable in studies in vitro. The results obtained with the chick embryo in ovo confirmed the suitability of this system for embryotoxicity testing.
Dusek
Z
Z
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague. zdik@biomed.cas.cz
Holejsovská
I
I
Novotná
B
B
Zemanová
Z
Z
D000014:Abnormalities, Drug-Induced; D000818:Animals; D002642:Chick Embryo / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D015946:Ethylene Dibromide / Q000378:metabolism / Q000494:pharmacology / Q000633:toxicity*; D013723:Teratogens / Q000633:toxicity*
D013723:Teratogens; D015946:Ethylene Dibromide