Effect of anti-P-selectin monoclonal antibody on renal ischemia/reperfusion injury in rats.
OBJECTIVE
To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats.


METHODS
Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used.


RESULTS
Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group.


CONCLUSIONS
Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.
Zhou
T
T
Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Li
X
X
Wu
P
P
Zhang
D
D
Zhang
M
M
Chen
N
N
Dong
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007674:Kidney Diseases / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D019007:P-Selectin / Q000097:blood / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D019007:P-Selectin; D012333:RNA, Messenger; D003404:Creatinine