Signal amplification by substrate recycling on polyaniline/lactate oxidase/lactate dehydrogenase bienzyme electrodes.
The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate at lower concentrations (0.1-1 mM). The PANI/LOD/LDH bienzyme electrodes were found to be stable for about 21 d at 4-10 degrees C.
Chaubey
A
A
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India. achaubey@csnpl.ren.nic.in
Pande
K K
KK
Pandey
M K
MK
Singh
V S
VS
D000814:Aniline Compounds / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry; D001709:Biotechnology; D004305:Dose-Response Relationship, Drug; D004566:Electrodes; D005947:Glucose / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D006899:Mixed Function Oxygenases / Q000737:chemistry*; D008956:Models, Chemical; D013696:Temperature; D014527:Uric Acid / Q000737:chemistry
D000814:Aniline Compounds; C416807:polyaniline; D014527:Uric Acid; D019344:Lactic Acid; D018698:Glutamic Acid; D006899:Mixed Function Oxygenases; D007770:L-Lactate Dehydrogenase; C021497:lactate 2-monooxygenase; D005947:Glucose; D001205:Ascorbic Acid