[EBV latent membrane protein 1 induces p53 expression via NF-kappa B in nasopharyngeal carcinoma].
OBJECTIVE
To ascertain if EBV-encoded latent membrane protein 1 (LMP1) induces p53 expression via NF-kappa B signaling.


METHODS
A nasopharyngeal carcinoma cell line, Tet-on-LMP1 HNE2, transfected with LMP1, the expression of which was regulated by tetracycline, was used in this study. Functional activity of NF-kappa B was determined by luciferase reporter assay and expression of p53 and bcl-2 was detected by Western blot.


RESULTS
LMP1 induced p53 expression via NF-kappa B signaling pathway. Induction of p53 expression could be blocked by phosphorothiate analogs of antisense oligonucleotides to NF-kappa B p65 and LMP1, but not by NF-kappa B p50. However, it seemed that LMP1 had no influence on bcl-2 expression in nasopharyngeal carcinoma.


CONCLUSIONS
Induction expression of p53 by EBV-encoded LMP1 implies that p53 may act as a mediator in apoptosis triggered by LMP1, which brings about a complex balance in nasopharyngeal carcinogenesis.
Liao
W
W
Cancer Research Institute, Hunan Medical University, Changsha, Hunan 410078, China.
Tang
M
M
Yin
L
L
D004854:Herpesvirus 4, Human / Q000737:chemistry*; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology*; D052002:NF-kappa B p50 Subunit; D009303:Nasopharyngeal Neoplasms; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D015398:Signal Transduction; D051996:Transcription Factor RelA; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000494:pharmacology*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D051996:Transcription Factor RelA; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins