[Isolation, purification, and separation of the complex preparation of extracellular proteinases with fibrinolytic and anticoagulant properties from Aspergillus ochraceus 513].
The extracellular proteinase complex of the microscopic fungus Aspergillus ochraceus 513 was isolated, purified, and separated by affinity chromatography on bacillichin-silochrom and subsequent column chromatography on DEAE-Toyopearl 650 M. The extracellular enzyme of the protein C activator type had a molecular mass of 36.5 kDa and activity close to that of the Agkistrodon snake venom protein C activator. The fibrinolytic and anticoagulant activities of the enzyme were investigated.
Batomunkueva
B P
BP
Department of Microbiology, Faculty of Biology, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
Egorov
N S
NS
D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification*; D001235:Aspergillus ochraceus / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry / Q000302:isolation & purification*; D005110:Extracellular Space / Q000737:chemistry; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008970:Molecular Weight
D000925:Anticoagulants; D002364:Caseins; D005343:Fibrinolytic Agents; D010450:Endopeptidases
Vydelenie, ochistka i razdelenie kompleksnogo preparata vnekletochnykh proteinaz Aspergillus ochraceus 513 s fibrinoliticheskimi i antikoaguliantnymi svoĭstvami.