[Electrophoretic analysis of protein composition in gingival crevicular fluid].
OBJECTIVE
To seek the protein composition related to periodontal diseases.


METHODS
86 gingival crevicular fluid (GCF) samples were collected by rinsing the sulgingival sulci from subjects with healthy gingiva (20), gingivitis (24) and periodontitis (42). The protein composition were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on a Phast System. The gels were scanned and analyzed using Phast Image with computer.


RESULTS
In inflammatory condition, the serum derived proteins with MW 82,000, 77,000, 17,000 occurred more frequently than in healthy gingiva, and the occurrence and abundance of non-serum derived proteins with MW 41,000, 21,000, 12,000 were also increased in inflammatory condition compared with healthy gingiva.


CONCLUSIONS
The high levels of proteins with MW 82,000, 41,000, 21,000, 12,000 are close related to the periodontal inflammatory status.
Zhou
H
H
School of Stomatology, Beijing Medical University, Beijing 100081.
Meng
H
H
Chen
Z
Z
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008875:Middle Aged; D008970:Molecular Weight; D010518:Periodontitis / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*
D001798:Blood Proteins; D011506:Proteins