[Transferring of mdr1 gene into murine bone marrow cells and its protective effects on hematopoietic cells against toxicities of anticancer agent].
OBJECTIVE
To investigate the protective effects of mdr1 gene on hematopoietic cells against anticancer agents and to explore an effective approach to prevent myelosuppression due to chemotherapy.


METHODS
mdr1 gene was transferred into murine bone marrow cells with plasmid pHaMDR1/A by the mediation of lipofection. Pgp, a product of mdr1 gene expression, and its function were detected by flow cytometry, immunohistochemistry, and rhodamine test respectively. Also, the resistance of transferred murine bone marrow cells to anticancer agents was tested by colony-forming unit test after exposure to anticancer agents and by bone marrow transplantation murine model.


RESULTS
mdr1 gene was successfully transferred and expressed in bone marrow cells. Transferred murine bone marrow cells were resistant to anticancer agents both in vitro and in vivo.


CONCLUSIONS
Transfection of mdr1 gene into hematopietic cells can protect bone marrow cells against the toxicities of anticancer agents.
Chen
L
L
Department of Pulmonary Medicine, General Hospital of PLA, Beijing 100853.
Liu
Y
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000187:drug effects / Q000502:physiology*; D002450:Cell Communication; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents