title: Production of viral progeny in insect cells undergoing apoptosis induced by a mutant Anticarsia gemmatalis nucleopolyhedrovirus.
abstract: The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) is the most successful viral biopesticide in use worldwide. We have demonstrated that despite widespread apoptosis and no protein synthesis at 48 h p.i., UFL-AG-286 cells infected with a mutant of AgMNPV (vApAg), produced significant amounts of budded virus (BVs) and viral DNA late in infection. However, a different susceptible cell line (BTI-Tn5B 1-4) showed no signs of apoptosis and produced 3.5 times more budded virus when infected with vApAg. A comparison of DNA from AgMNPV and vApAg digested with the same restriction enzymes showed differences in the restriction pattern, indicating that the vApAg phenotype might be due to a mutation in a gene or genes responsible for directly or indirectly inhibiting apoptosis in UFL-AG-286 cells.
authors.affiliation: Embrapa Recursos Genéticos e Biotecnologia, Cx. Postal 02372, Brasília, DF, 70849-970, Brasil.
authors.forename: M E
authors.initials: ME
authors.lastname: de Castro
authors.forename: B M
authors.initials: BM
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016679:Genome, Viral; D007313:Insecta / Q000166:cytology / Q000821:virology*; D008858:Microscopy, Phase-Contrast; D009154:Mutation; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010572:Pest Control, Biological; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins