title: Peptide-pulsed splenic dendritic cells prime long-lasting CD8(+) T cell memory in the absence of cross-priming by host APC.
abstract: Immunization with cells expressing endogenous antigens can stimulate long-lived CD8(+) T cell memory. In many cases, the response is also stimulated by host antigen-presenting cells (APC) that have processed antigen from internalized apoptotic cells or cell fragments. This study investigated whether immunization with peptide-pulsed dendritic cells (DC) could prime long-lasting, peptide-specific CD8(+) T cell immunity in the absence of cross-priming by host APC. C57BL / 6 female mice immunized with syngeneic male splenic DC pulsed with the H-2K(b)-restricted ovalbumin peptide OVA(257 - 264) made memory CD8(+) CD44(high) T cell responses to OVA(257 - 264) and the male antigen HY more than 1 year after immunization. Establishment and maintenance of peptide-specific CD8(+) T cell memory did not require antibody or B cells. Immunization of H-2(bxd) mice with OVA(257 - 264)-pulsed minor-incompatible H-2(b) or H-2(d) DC demonstrated that CD8(+) T cells were primed exclusively by the injected cells, and not by peptide transferred to host APC, even though there was very effective cross-priming for CD8(+) T cell responses to the minor antigens expressed by the DC. Thus peptide-pulsed DC can prime long-lasting CD8(+) memory responses without any requirement for cross-priming by other APC.
authors.affiliation: Basel Institute for Immunology, Basel, Switzerland. alexandra_livingstone@urmc.rochester.edu
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Livingstone
authors.forename: Marina
authors.initials: M
authors.lastname: Kuhn
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002470:Cell Survival; D017690:Cell Transplantation; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004527:Egg Proteins / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D006182:H-Y Antigen / Q000276:immunology; D007114:Immunization; D007156:Immunologic Memory / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens / Q000276:immunology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010446:Peptide Fragments; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D000941:Antigens; D004527:Egg Proteins; D006182:H-Y Antigen; D015778:Minor Histocompatibility Antigens; C110366:OVA-8; D010446:Peptide Fragments; D010455:Peptides; D010047:Ovalbumin