title: Enzyme catalysis in reverse micelles.
abstract: Water in oil microemulsions with reverse micelles provide an interesting alternative to normal organic solvents in enzyme catalysis with hydrophobic substrates. Reverse micelles are useful microreactors because they can host proteins like enzymes. Catalytic reactions with water insoluble substrates can occur at the large internal water-oil interface inside the microemulsion. The activity and stability of biomolecules can be controlled, mainly by the concentration of water in these media. With the exact knowledge of the phase behaviour and the corresponding activity of enzymes the application of these media can lead to favourable effects compared to aqueous systems, like hyperactivity or increased stability of the enzymes.
authors.affiliation: Henkel KGaA, WEV, Düsseldorf, Germany. Bernhard.Orlich@denotes.henkel.de
authors.forename: Bernhard
authors.initials: B
authors.lastname: Orlich
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schomäcker
mesh_terms: D001709:Biotechnology / Q000191:economics / Q000379:methods*; D002384:Catalysis; D002498:Centrifugation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D004655:Emulsions; D004798:Enzymes / Q000378:metabolism*; D004800:Enzymes, Immobilized; D005374:Filtration; D005782:Gels; D057927:Hydrophobic and Hydrophilic Interactions; D008823:Micelles; D009821:Oils; D012995:Solubility; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water
chemical_list: D004655:Emulsions; D004798:Enzymes; D004800:Enzymes, Immobilized; D005782:Gels; D008823:Micelles; D009821:Oils; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water