[The effect of some mutations in the Trl gene on mitosis in embryonal and larval tissues and egg chamber morphology in Drosophila melanogaster].
A study was made of three insertional mutations (Trl13C, Trls2325, and TrlEP(3)3184) located in the second intron of the Trithorax-like (Trl) gene for the GAGA transcription factor (GAF). Their cytological effects were analyzed in oogenesis, early embryonic development, and in larval development (96-108 h) in cells of nervous ganglia and imaginal disks. Notwithstanding an interallelic difference in expression, all three P-element insertions proved to be dominant as far as the examined parameters were concerned. The most substantial defects were the formation of "granular" chromatin during the interphase and mitosis and high proportions of cells with hypercondensed chromatin (which were arrested at the G2/M boundary) and cells with abnormal chromosome segregation. A higher frequency of egg chambers with trophocytes defective in number and in chromatin condensation was observed in females carrying the mutant Trl gene. The defects were assumed to result from poor coordination of the chromosome and cell cycles and, including, the nuclear and centrosomal cycles in embryonic development and the cycles of chromosome condensation and spindle formation in cells of larval imaginal disks and nervous ganglia.
Trunova
S A
SA
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Fedorova
S A
SA
Lebedeva
L I
LI
Bulgakova
N A
NA
Omel'ianchuk
L V
LV
Katokhin
A V
AV
Baricheva
E M
EM
D000818:Animals; D002875:Chromosomes; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000235:genetics* / Q000254:growth & development; D005314:Embryonic and Fetal Development / Q000235:genetics*; D007814:Larva / Q000166:cytology*; D008938:Mitosis / Q000235:genetics*; D009154:Mutation; D010063:Ovum; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila
Vliianie nekotorykh mutatsiĭ po genu Trl na mitoz v émbrional'noĭ i lichinochnykh tkaniakh i morfologiiu iaĭtsevykh kamer u Drosophila melanogaster.