Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning.
The presence of galactose alpha-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the alpha-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.
Lai
Liangxue
L
Department of Animal Science, University of Missouri, Columbia, MO 65211, USA.
Kolber-Simonds
Donna
D
Park
Kwang-Wook
KW
Cheong
Hee-Tae
HT
Greenstein
Julia L
JL
Im
Gi-Sun
GS
Samuel
Melissa
M
Bonk
Aaron
A
Rieke
August
A
Day
Billy N
BN
Murphy
Clifton N
CN
Carter
David B
DB
Hawley
Robert J
RJ
Prather
Randall S
RS
D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D019976:Cloning, Organism; D004624:Embryo Transfer; D005260:Female; D005333:Fetus; D005347:Fibroblasts; D005700:Galactosyltransferases / Q000235:genetics*; D018390:Gene Targeting; D005822:Genetic Vectors; D008297:Male; D016254:Mutagenesis, Insertional; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011995:Recombination, Genetic; D013552:Swine; D013556:Swine, Miniature / Q000196:embryology / Q000235:genetics*; D014162:Transfection
D005700:Galactosyltransferases; C047006:N-acetyllactosaminide alpha-1,3-galactosyltransferase