A one-step method for genetic transformation of non-piliated Neisseria meningitidis.
A one-step method to chemically transform non-piliated Neisseria meningitidis strains previously resistant to conventional electrochemical transformation procedures has been developed. This method has been used to generate genetically engineered meningococcal strains disrupted in the structural rmpM gene encoding Rmp.
Bogdan
John A
JA
Department of Molecular Biology, Baxter Healthcare Corporation, 10150 Old Columbia Road, Columbia, MD 21046-2358, USA. John_Bogdan@Baxter.com
Minetti
C A S A
CA
Blake
M S
MS
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D009345:Neisseria meningitidis / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C499670:RmpM protein, Neisseria