A preservation method that allows recovery of intact RNA from tissues dissected by laser capture microdissection.
We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis.
Parlato
Rosanna
R
Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoles, Italy.
Rosica
Annamaria
A
Cuccurullo
Vincenzo
V
Mansi
Luigi
L
Macchia
Paolo
P
Owens
James D
JD
Mushinski
J Frederic
JF
De Felice
Mario
M
Bonner
Robert F
RF
Di Lauro
Roberto
R
D000818:Animals; D015152:Blotting, Northern; D015925:Cryopreservation / Q000379:methods; D004210:Dissection / Q000379:methods*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007834:Lasers; D051379:Mice; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013395:Sucrose; D013964:Thyroid Neoplasms / Q000235:genetics / Q000473:pathology; D014021:Tissue Preservation / Q000379:methods*
D012333:RNA, Messenger; D013395:Sucrose; D012313:RNA