Identification of a novel HLA-DQB1*06 allele, DQB1*0618.
We report here the identification of a novel DQB1*06 allele, DQB1*0618, found in a bone marrow donor. The new allele was detected during routine DNA-based HLA typing by an ambiguous pattern of probe hybridization, obtained by polymerase chain reaction using sequence-specific oligonucleotides (PCR-SSO). Molecular cloning and sequencing confirmed that the new allele is identical to DQB1*0609 at exon 2 except for 3 nucleotide substitutions at positions 353, 356 and 367, also found in other alleles. These nucleotide changes may explain its anomalous reactivity.
Casamitjana
N
N
Servei d'Immunohematologia, Centre de Transfusió i Banc de Teixits, Barcelona, Spain.
Gil
J
J
Campos
E
E
Santos
M
M
Nogués
N
N
Ribera
A
A
Palou
E
E
D000483:Alleles; D001483:Base Sequence; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen