title: [1,25(OH)2 vitamin D3 induces IL-1 alpha mRNA expression from osteoblastic-like and bone marrow co-culture cells].
abstract: OBJECTIVE
  The purpose of this research is to investigate the relationship between 1,25(OH)2 Vitamin D3[1,25(OH)2D3] and Interleukin-1 (IL-1) in regulating osteoclast formation. The result may enrich our knowledge about biological mechanisms of periodontal tissue remodeling during orthodontic tooth movement.
  
  
  METHODS
  We found IL-1 alpha mRNA expression in bone marrow and osteoblastic cell co-cultures by semi-quantitative RT-PCR.
  
  
  RESULTS
  1,25(OH)2D3 increased IL-1 alpha mRNA expression from this co-culture cells in a dose dependent manner, and the expression reached its peak in day 2 and decreased thereafter.
  
  
  CONCLUSIONS
  These results demonstrate that IL-1 alpha involves in osteoclast formation induced by 1,25(OH)2D3. And we propose IL-1 alpha may stimulate the proliferation of osteoclast progenitor during the first 4 days of total 6 days culture period.
authors.affiliation: School of Stomatology, Beijing Medical University, Beijing 100081.
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Lorenzo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology*; D018920:Coculture Techniques; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D002117:Calcitriol