[A preliminary study on translocation of the immunoglobulin heavy chain gene in KM3 myeloma cell line].
OBJECTIVE
To identify the IgH translocation in KM3 myeloma cell line established from a Chinese patients.


METHODS
Southern blotting was performed with dig-labeled probes specific for IgH switch regions to identify and distinguish IgH switch recombination events.


RESULTS
Besides a 10.0 kb Hind III fragment being able to hybridize with both 5' S mu and 3' S mu probes as that in placenta genome DNA. There was a 5.6 kb Hind III fragment in KM3 genome DNA could only hybridize with the 3' S mu probe.


CONCLUSIONS
There was an illegitimate switch recombination fragment containing 3' S mu region in the KM3 myeloma cell line, which was caused by the IgH translocation involving the IgH S mu region.
Shen
H
H
Department of Hematology, Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, China.
Hou
J
J
Liu
H
H
D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D007143:Immunoglobulin Heavy Chains; D004247:DNA; D015247:Deoxyribonuclease HindIII