title: Chromosomal imbalances in lymphoid tumors of the orbit.
abstract: OBJECTIVE
  To identify chromosomal gains and losses in lymphoid tumors of the orbit and to examine whether such abnormalities are related to orbital presentation, disease severity, or risk for recurrent disease.
  
  
  METHODS
  Biopsy specimens from 26 patients were examined by histomorphologic and immunohistochemical analysis. Lymphomas were classified according to the Revised European-American Lymphoma Classification. Chromosomal imbalances were detected by high-resolution comparative genomic hybridization (CGH). Clinical data were obtained by retrospective evaluation of medical records.
  
  
  RESULTS
  Chromosomal imbalances were detected in 0 of 6 patients with idiopathic orbital inflammation, 0 of 2 with benign reactive lymphoid hyperplasia, 3 of 3 with highly malignant diffuse large B-cell lymphoma, 4 of 10 with marginal zone B-cell lymphoma, 0 of 1 with chronic lymphatic leukemia lymphoma, and 1 of 4 with immunocytoma. Among the low-grade malignancies, chromosomal imbalances were seen in 1 of 9 at stage IAE, 2 of 3 at stage IIE, and 2 of 3 at stage IVE. Chromosomal imbalances were observed in all primary tumors from the five patients that later developed recurrent disease. In 14 of 23 imbalances with intrachromosomal breaks outside the centromere region, the breaks were present at bands with known fragile sites. No chromosomal imbalances specific for orbital presentation were detected.
  
  
  CONCLUSIONS
  Chromosomal imbalances were seen mainly in orbital lymphomas that were either highly malignant or at an advanced stage. CGH analysis of orbital lymphomas could be prognostically relevant, but further studies are required to confirm this notion.
authors.affiliation: Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway.
authors.forename: Gro Oddveig
authors.initials: GO
authors.lastname: Ness
authors.forename: Helle
authors.initials: H
authors.lastname: Lybaek
authors.forename: Jarle
authors.initials: J
authors.lastname: Arnes
authors.forename: Eyvind
authors.initials: E
authors.lastname: Rødahl
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009918:Orbital Neoplasms / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm