[Antitumor effect of radiation combined with tumor draining lymphocytes on human ACC-M cell in vitro].
OBJECTIVE
To find whether there is any synergistic effect of radiation combined with interleukin-2(IL-2) activated tumor draining lymph nodes lymphocytes (DNL) from oral-carcinoma patients on high-lung metastatic salivary adenoid cystic carcinoma cell line(ACC-M).


METHODS
Colony-forming test was used to investigate antitumor effect and analyzed using linear-quadratic(LQ) equation and single hit multi targets equation.


RESULTS
The ratio of effect to targets was 25:1. The cytotoxicity of DNL was 49.06%. Radiation combined with DNL showed higher antitumor activity compared with radiation alone, alpha value, Dq and S2 were 0.7688 and 0.342 0; 1.5901 and 0.5995; 0.4481 and 0.1135 respectively(P < 0.05).


CONCLUSIONS
It indicates that in initial region of survival curve, DNL significantly increased sublethal damage on ACC-M.
Cai
Y
Y
Department of Oral Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Qiu
W
W
He
R
R
D003528:Carcinoma, Adenoid Cystic / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D002470:Cell Survival / Q000276:immunology / Q000528:radiation effects; D003602:Cytotoxicity, Immunologic; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D012468:Salivary Gland Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D014407:Tumor Cells, Cultured