Interactions among challenges of hydromineral balance, angiotensin-converting enzyme, and cystine aminopeptidase.
Enzymatic cleavage of some peptides could be included among the mechanisms of water-electrolyte homeostasis. To test this hypothesis, the angiotensin-converting activity (ACE) of plasma and the L-cystine-di-beta-naphthylamidase activity (CAP) of plasma and of soluble and particulate fractions from different areas of the central nervous system (CNS) were investigated in rats submitted to treatments eliciting hydromineral imbalance. CAP in the CNS was unchanged by hydromineral challenges. The correlations observed between plasma osmolality and CAP, and plasma CAP and ACE suggested a contribution of these activities to the restoration of basal water-electrolyte and blood pressure conditions through the hydrolysis of vasopressin, oxytocin, angiotensin I and bradykinin.
Silveira
P F
PF
Laboratory of Pharmacology, Instituto Butantan, Av. Vital Brazil 1500, 05503-900, São Paulo, SP, Brazil. pefesil@bol.com.br
Irazusta
J
J
Gil
J
J
Agirregoitia
N
N
Casis
L
L
D000804:Angiotensin II / Q000096:biosynthesis; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D001920:Bradykinin / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism; D010122:Cystinyl Aminopeptidase / Q000097:blood / Q000378:metabolism*; D008297:Male; D010121:Oxytocin / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010446:Peptide Fragments; D000804:Angiotensin II; D001127:Arginine Vasopressin; D010121:Oxytocin; D010122:Cystinyl Aminopeptidase; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin