[p53 gene mutation determined by single-strand conformational polymorphism and DNA sequencing analysis in pleomorphic adenoma of salivary gland].
OBJECTIVE
To detect p53 exon 6 mutation pattern in pleomorphic adenoma of salivary gland.


METHODS
Single-strand conformational polymorphism (SSCP) and DNA sequecing were used.


RESULTS
SSCP analysis showed that 6 of 11 cases with SSCP positive were exon 6 abnormal. Comparison of the SSCP results with DNA sequecing, 4 cases with p53 exon 6 mutations were SSCP positive. Mutation pattern included base substitution (point mutation, G-->T, T-->G) and frame-shift mutation (base insertion and base loss).


CONCLUSIONS
Poly-codon and poly-pattern mutations appeared in p53 exon 6. PCR-SSCP was a convenient method to detecte gene mutation.
Wang
J
J
School of Stomatology, Hebei Medical University, Shijiazhuang 050017.
Dong
F
F
Yong
P
P
D008949:Adenoma, Pleomorphic / Q000235:genetics / Q000473:pathology*; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000473:pathology*; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53