title: Immunosorbent for removal of b2-microglobulin from human blood plasma.
abstract: Immunosorbent removing beta2-microglobulin from human blood plasma was synthesized on the basis of sheep monospecific polyclonal antibodies to beta2-microglobulin and conditions ensuring effective regeneration of the immunosorbent for its repeated use were selected. Relationships between adsorption capacity and the volume of immobilized ligand, antigen concentration in the solution, and duration of incubation were studied. The adsorbent can be used for effective and specific removal of beta2-microglobulin from the plasma of patients on chronic hemodialysis.
authors.affiliation: Institute of Experimental Cardiology, Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow.
authors.forename: I V
authors.initials: IV
authors.lastname: Shabunina
authors.forename: O I
authors.initials: OI
authors.lastname: Afanas'eva
authors.forename: S N
authors.initials: SN
authors.lastname: Pokrovskii
mesh_terms: D000327:Adsorption; D000818:Animals; D002846:Chromatography, Affinity; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007164:Immunosorbents / Q000494:pharmacology*; D007676:Kidney Failure, Chronic / Q000097:blood*; D008024:Ligands; D006435:Renal Dialysis; D012756:Sheep; D001613:beta 2-Microglobulin / Q000097:blood*
chemical_list: D007164:Immunosorbents; D008024:Ligands; D001613:beta 2-Microglobulin