title: In vitro effect of gelatins on murine cell proliferation.
abstract: Porcine skin (PS) gelatin suppressed proliferation of a murine hepatic cell carcinoma cell line, MH134, a murine fibrosarcoma cell line, Meth A and a murine T cell lymphoma cell line, RL Male 1. The magnitude of suppression of the proliferation by cold water fish skin (CWFS) or bovine bone (BB) gelatin was lower than that by PS gelatin. On the other hand, BB gelatin stimulated proliferation of murine spleen cells. The magnitude of stimulation of the proliferation by CWFS gelatin was lower than that by BB gelatin. PS gelatin slightly suppressed proliferation of murine spleen cells. PS gelatin induced apoptosis but not necrosis of MH134 tumor cells. CWFS gelatin induced weaker apoptosis of the cells than PS gelatin. DNA histogram indicated that PS and CWFS gelatins acted on MH134 tumor cells to increase ratios of G2 + M-phase.
authors.affiliation: Department of Surgery, Aichi-Gakuin University Hospital, 2-11, Suemori-dori, Chikusa-ku, Nagoya 464-8651, Japan. taku@dpc.aichi-gakuin.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: H
authors.initials: H
authors.lastname: Nagata
authors.forename: N
authors.initials: N
authors.lastname: Paeng
authors.forename: M
authors.initials: M
authors.lastname: Sano
authors.forename: R
authors.initials: R
authors.lastname: Sasanabe
authors.forename: I
authors.initials: I
authors.lastname: Horikoshi
authors.forename: N
authors.initials: N
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001842:Bone and Bones / Q000737:chemistry; D002417:Cattle; D002455:Cell Division / Q000187:drug effects*; D005260:Female; D005354:Fibrosarcoma / Q000473:pathology; D005399:Fishes; D016195:G2 Phase / Q000187:drug effects; D005780:Gelatin / Q000302:isolation & purification / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000473:pathology; D008297:Male; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000737:chemistry; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013552:Swine; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
chemical_list: D005780:Gelatin