[Detecting mycobacteria and their L-forms in peripheral blood from pulmonary tuberculosis patients by cultivation with hemolyzed-centrifugated blood in liquid medium].
OBJECTIVE
To establish a new method of detecting mycobacteria and their L-forms in blood.


METHODS
65 samples of peripheral blood from patients with pulmonary tuberculosis were cultivated directly or cultivated with hemolyzed-centrifugated blood sediments in 92-3TB and 92-3TBL liquid medium, and the cultures were staining with immunoenzyme technique (ABC method).


RESULTS
In 65 specimens, the positive rates of mycobacteria and their L-forms were 15%, 26% respectively with total isolation rate of 32%. In cultivating with hemolyzed-centrifugated blood the positive rate was much higher than direct cultural method (P < 0.05). In sputum the positive rates of acid-fast bacilli and their L-forms were 38% and 20% respectively, with total isolation rate of 52%, higher than that in peripheral blood (P < 0.05), and the total positive rate of combinative detection of blood and sputum salmples was 65%.


CONCLUSIONS
Mycobacteria and their L-forms exist in peripheral blood of patients with pulmonary tuberculosis, and mainly in L-forms. Cultivation with hemolyzed-centrifugated blood in liquid medium and staining with immunoenzyme technique are valuable in routine detection of mycobacteria and their L-forms in peripheral blood. The positive rate of bacterial culture of pulmonary tuberculosis could be increased in combination with sputum mycobacteria and their L-forms examination.
Zhu
M
M
Central Laboratory, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Xia
P
P
Zhang
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002498:Centrifugation; D005260:Female; D006461:Hemolysis; D006801:Humans; D007740:L Forms / Q000302:isolation & purification*; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000302:isolation & purification*; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*