title: Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1.
abstract: Adenovirus type 2 (Ad2) imports its DNA genome through the nuclear pore complex (NPC) of cells in interphase for viral production. Here we identify the NPC-filament protein CAN/Nup214 as a docking site for incoming Ad2 capsids. Binding to CAN is independent of cytosolic factors. Capsids disassemble at NPCs to free their DNA for import. This process requires binding of nuclear histone H1 to the stably docked capsids and involves H1-import factors, restricting this irreversible process to the proximity of the nucleus. Our results provide a molecular mechanism for disassembly of Ad2 and reveal an unexpected function of histone H1 in virus-mediated DNA import.
authors.affiliation: University of Zürich, Institute of Zoology, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
authors.forename: L C
authors.initials: LC
authors.lastname: Trotman
authors.forename: N
authors.initials: N
authors.lastname: Mosberger
authors.forename: M
authors.initials: M
authors.lastname: Fornerod
authors.forename: R P
authors.initials: RP
authors.lastname: Stidwill
authors.forename: U F
authors.initials: UF
authors.lastname: Greber
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000256:Adenoviridae / Q000235:genetics*; D000595:Amino Acid Sequence; D000906:Antibodies / Q000494:pharmacology; D002213:Capsid / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D036022:Capsid Proteins; D004279:DNA, Viral / Q000493:pharmacokinetics*; D006657:Histones / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms; D008969:Molecular Sequence Data; D022022:Nuclear Pore / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D014407:Tumor Cells, Cultured; D028901:alpha Karyopherins / Q000378:metabolism; D028961:beta Karyopherins / Q000378:metabolism
chemical_list: D000906:Antibodies; D036022:Capsid Proteins; D004279:DNA, Viral; D006657:Histones; C085914:NUP214 protein, human; D028861:Nuclear Pore Complex Proteins; D028901:alpha Karyopherins; D028961:beta Karyopherins; C018315:hexon capsid protein, Adenovirus