Bactericidal effect of delmopinol on attached and planktonic Streptococcus sanguinis cells.
The aim of this investigation was to determine the antibacterial effect of varying concentrations of delmopinol-HCl on attached as well as on planktonic Streptooccus sanguinis cells in vitro. In addition, a possible antiadhesive effect on attached micro-organisms was to be investigated. S. sanguinis cells were allowed to attach to glass surfaces. These as well as planktonic cells were exposed to delmopinol-HCI in concentrations ranging from 0.2% to 0.00005% for 2 min. The percentage of vital bacteria was calculated by means of a fluorescence staining method. Total counts of attached bacteria were performed to determine any possible detaching effect by the delmopinol-HCl. The CFU were determined for the planktonic bacteria. Attached as well as planktonic bacteria showed a marked decrease in vitality following exposure to 0.2% delmopinol-HCl. After exposure to 0.05% this was only the case with the attached microorganisms. The total number of attached bacteria was not reduced by the delmopinol treatment. During initial dental biofilm formation, delmopinol-HCl causes a bactericidal effect when applied in concentrations of 0.05% and higher.
Burgemeister
S
S
Department of Conservative Dentistry, School of Dental Medicine, University of Tübingen, Germany. s_burgemeister@yahoo.de
Decker
E M
EM
Weiger
R
R
Brecx
M
M
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D015169:Colony Count, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests; D009025:Morpholines / Q000494:pharmacology*; D009067:Mouthwashes / Q000494:pharmacology*; D012463:Saliva / Q000382:microbiology; D013298:Streptococcus sanguis / Q000187:drug effects*
D000891:Anti-Infective Agents, Local; D009025:Morpholines; D009067:Mouthwashes; C073738:delmopinol