Atrazine degradation by bioaugmented sediment from constructed wetlands.
The potential to establish pesticide biodegradation in constructed wetland sediment was investigated. Under microcosm conditions, bioaugmentation of sediment with small quantities of an atrazine spill-site soil (1:100 w/w) resulted in the mineralization of 25-30% of 14C ethyl atrazine (1-10 microg g(-1) sediment) as 14CO2 under both unsaturated and water-saturated conditions; atrazine and its common metabolites were almost undetectable after 30 days incubation. By comparison, unbioaugmented sediment supplemented with organic amendments (cellulose or cattail leaves) mineralized only 2-3% of 14C ethyl atrazine, and extractable atrazine and its common metabolites comprised approximately 70% of the original application. The population density of atrazine-degrading microorganisms in unbioaugmented sediment was increased from approximately 10(2)/g to 10(4)/g by bioaugmentation (1:100 w/w), and increased by another 60-fold (6.0x10(5) g(-1)) after incubation with 10 microg g(-1) of atrazine. A high population of atrazine degraders (approximately 10(6) g(-1)) and enhanced rates of atrazine mineralization also developed in bioaugmented sediment after incubation in flooded mesocosms planted with cattails (Typha latifolia) and supplemented with atrazine (3.2 mg l(-1), 1 microg g(-1) sediment). In the absence of atrazine, neither the population of atrazine degraders, nor the atrazine mineralizing potential of bioaugmented sediment increased, regardless of the presence or absence of cattails. Bioaugmentation might be a simple method to promote pesticide degradation in nursery run-off channeled through constructed wetlands, if persistence of degraders in the absence of pesticide is not a serious constraint.
Runes
H B
HB
Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis 97331-3804, USA.
Jenkins
J J
JJ
Bottomley
P J
PJ
D001280:Atrazine / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D006540:Herbicides / Q000378:metabolism*; D009922:Oregon; D010944:Plants; D012988:Soil Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D006540:Herbicides; D014874:Water Pollutants, Chemical; D001280:Atrazine