title: Molecular epidemiologic study of Mycoplasma genitalium infection in high risk populations of sexually transmitted diseases in China.
abstract: OBJECTIVE
  To detect Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) from several cities in China and to clarify the possible role of Mg in the pathogenesis of non-gonococcal urethritis.
  
  
  METHODS
  Polymerase chain reaction (PCR) based on 2 pairs of primers, one for Mg-Pa (adhesion protein) and the other for 16S-rRNA (Mycoplasma genera) was used to detect Mg infection. Urogenital specimens of different high risk populations and urethritis patients, were collected from the Guangdong, Kunming, Shanghai, Nanjing, and Changzhou areas.
  
  
  RESULTS
  The positive detection rate of Mg-DNA in high risk populations of STDs was significantly higher than that of the control group (chi 2 = 7.82, P < 0.01). The positive detection rates in Guangdong STD clinics and promiscuous persons from Kunming were higher than those from the Shanghai, Nanjing, and Changzhou areas (chi 2 = 8.54, P < 0.01 and chi 2 = 5.89, P < 0.05). Mg DNA could be detected in those patients without other relevant pathogens. Some patients were simultaneously infected with Mg and other microbes, such as Chlamydia trachomatis and/or Ureaplasma urelyticum. The positive Mg-DNA detection rate in patients with urethritis symptoms was higher than in patients without the symptoms (chi 2 = 11.68, P < 0.01).
  
  
  CONCLUSIONS
  Mg infection exists in high risk populations of STDs in China, and the Mg infection rate is different among different high risk groups.
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: D
authors.initials: D
authors.lastname: Luo
authors.forename: W
authors.initials: W
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Liang
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.forename: W
authors.initials: W
authors.lastname: Xu
mesh_terms: D000293:Adolescent; D000328:Adult; D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000453:epidemiology*; D012306:Risk; D015231:Sexually Transmitted Diseases, Bacterial / Q000453:epidemiology*
chemical_list: D004269:DNA, Bacterial