title: Dual-substrate utilization by Bordetella pertussis.
abstract: To improve the cultivation of Bordetella pertussis and take advantage of the newest techniques in monitoring and control, a quantitative description of substrate utilisation is necessary. Growth of the organism is limited by two main substrates. However neither interactive nor non-interactive modelling seem appropriate. A model that combines essential and enhanced kinetics was developed based on experimental observation. Instead of fitting all model parameters at once, a step-wise experimentation procedure was used. Finally two cultivations showed the accuracy of the model.
authors.affiliation: National Institute of Public Health and the Environment, Laboratory for Product and Process Development, Bilthoven, The Netherlands. Ronald.Neeleman@rivm.nl
authors.forename: R
authors.initials: R
authors.lastname: Neeleman
authors.forename: M
authors.initials: M
authors.lastname: Joerink
authors.forename: C
authors.initials: C
authors.lastname: Beuvery
authors.forename: T
authors.initials: T
authors.lastname: van Boxtel
mesh_terms: D018533:Biomass; D001886:Bordetella pertussis / Q000254:growth & development* / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D019344:Lactic Acid; D018698:Glutamic Acid