[Comparison of susceptibility testing results of M. tuberculosis by different methods].
OBJECTIVE
To compare the susceptibility testing results of M. tuberculosis detected with different standards and methods.


METHODS
158 strains of M. tuberculosis isolated from clinical patients were detected for the drug sensitivity by Chinese standard and method (absolute concentration method), 1% resistance ratio and absolute concentration method of WHO/IUATLD standard.


RESULTS
The drug-resistant rate detected by the standard of WHO/IUATLD was 53.8%(85/158), while by the Chinese standard 50.0%(79/158). The drug-resistant rates to INH, SM, EMB and RFP by WHO/IUATLD standard were 41.8%(66/158), 34.2%(54/158), 22.1%(35/158) and 27.8%(44/158) respectively, whereas by the Chinese standard 34.2%(54/158), 30.4% (48/158), 18.4%(29/158) and 27.2%(43/158) respectively. There was no significant difference between the results detected with WHO/IUATLD and Chinese standard, except for the results of INH. There was no significant difference between the results detected with WHO/IUATLD standard by 1% resistant ratio and absolute concentration methods.


CONCLUSIONS
The results of drug-sensitivity were interfered by the detected standards but not by the tested methods.
Ding
B
B
Beijing Research Institute for Tuberculosis Control, Beijing 100035, China.
Zhao
T
T
Gai
Y
Y
D000995:Antitubercular Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D013045:Species Specificity
D000995:Antitubercular Agents