Occurrence of Listeria monocytogenes in food in Chile.
Out of 2145 food samples analysed 77 were found contaminated with Listeria monocytogenes in Santiago, Chile. Samples were: 603 ice-cream (3.5% contaminated), 256 soft cheese (0.8%), 155 hard cheese (0%), 229 baby milk bottles (0%), 634 processed meat products (3.6%) and 268 crustaceous shellfish (11.6%). Three different isolation media were used: for 318 samples, Modified McBride Agar (MMA), Lithium chloride Phenylethanol Moxalactam agar, and Polymyxin Acriflavine Lithium chloride Ceftazidime Aesculin Mannitol agar; for 1827 samples MMA was replaced by Listeria Selective Agar Oxford Formulation. Isolates were classified as follow: serovar 1/2a (25 isolates), serovar 4b (20), serovar 1/2b (19), serovar 3b (7), serovar 1/2c (2), untypable (4). A high variety of phagovars was detected although 52% of strains was untypable.
Cordano
A M
AM
Sección Microbiología de Alimentos, Instituto de Salud Pública de Chile, Santiago, Chile. acordano@ispch.cl
Rocourt
J
J
D001434:Bacteriophage Typing; D002677:Chile; D003470:Culture Media; D005506:Food Contamination; D005516:Food Microbiology; D015994:Incidence; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification*; D012703:Serotyping
D003470:Culture Media