title: Incidence of Clostridium perfringens in broiler chickens and their environment during production and processing.
abstract: During a calendar year, a study was conducted involving 16 broiler flocks on four different farms, two farms belonging to each of two major U.S. poultry integrators. As determined by the detection of Clostridium perfringens in fecal or cecal samples, 15 (94%) of the flocks became positive for this bacterial enteropathogen, and only one remained negative throughout the 6-to-8-wk rearing period. Paper pads beneath chicks that were transported from the hatchery to the rearing house were contaminated with C. perfringens in 15 (94%) of the flocks. When sampled biweekly through grow out, 13 of the flocks were C. perfringens positive at 2 wk of age. These results suggest that colonization of the intestinal tract of broilers by C. perfringens is an early event. Of the environmental samples, all but feed in the hopper were contaminated before placement for at least one of the rearing periods. All sample types were contaminated at some point during the 6-to-8-wk grow-out period. Of the on-farm environmental samples, the highest incidences (percentage positive) of C. perfringens were detected in wall swabs (53%), fan swabs (46%), fly strips (43%), dirt outside the house entrance (43%), and swabs of workers' boots (29%). Birds were usually transported to the processing plant in coops that were already contaminated with C. perfringens. In the plant, C. perfringens was isolated more frequently from samples of scald water than from those of chill water. Clostridium perfringens was recovered from broiler carcasses after chilling in 13 (81%) of the 16 flocks. The proportion of C. perfringens-positive carcasses for the contaminated flocks ranged from 8% to 68% with a mean of 30%.
authors.affiliation: Richard B. Russell Agricultural Research Center, Poultry Microbiological Safety Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA 30605, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Craven
authors.forename: N J
authors.initials: NJ
authors.lastname: Stern
authors.forename: J S
authors.initials: JS
authors.lastname: Bailey
authors.forename: N A
authors.initials: NA
authors.lastname: Cox
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D003015:Clostridium Infections / Q000453:epidemiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005511:Food Handling; D006799:Housing, Animal; D015994:Incidence; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology; D015144:Southeastern United States / Q000453:epidemiology; D014186:Transportation