title: The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo.
abstract: Intermolecular and intramolecular FRET between two spectrally overlapping green fluorescent protein variants fused to two different host proteins or at two different sites within the same protein offers a unique opportunity to monitor real-time protein-protein interactions or protein conformational changes. By using fluorescence digital imaging microscopy, one can visualize the location of green fluorescent proteins within a living cell and follow the time course of the changes in FRET corresponding to cellular events at a millisecond time resolution. The observation of such dynamic molecular events in vivo provides vital insight into the action of biological molecules.
authors.affiliation: Division of Molecular and Structural Biology, Ontario Cancer Institute, Toronto, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Truong
authors.forename: M
authors.initials: M
authors.lastname: Ikura
mesh_terms: D020013:Calcium Signaling; D010450:Endopeptidases / Q000378:metabolism; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence / Q000379:methods*; D010766:Phosphorylation; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
chemical_list: D008164:Luminescent Proteins; D046911:Macromolecular Substances; D011506:Proteins; D049452:Green Fluorescent Proteins; D010450:Endopeptidases