[Cloning and partially sequencing of mouse dentin sialophosphoprotein encoding mature protein].
OBJECTIVE
Cloning and partially sequencing of mouse dentin sialophosphoprotein (DSPP) encoding mature protein.


METHODS
Total RNA was extracted from the tooth germs of newborn mouse by acid guanidinium thiocyanata-phenol-chloroform method, the desired DNA product was obtained from the total RNA by RT-PCR with the primers including Oligo(dt) and two gene specific primers. The segment (about 3 Kbp) was inserted into pBluescript vector and the interesting plasmid was transformed into E. Coli host strain XL1-Blue. The double-stranded DNA of the positive clone was analyzed by restriction endonuclease mapping and DNA sequencing.


RESULTS
The restriction endonuclease map and sequence of mouse DSPP encoding mature protein were consistent with those of the published.


CONCLUSIONS
The mouse DSPP cDNA encoding mature protein was obtained for further study.
Hao
J
J
School of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Wang
P
P
Ma
S
S
D000818:Animals; D000831:Animals, Newborn; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003804:Dentin / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010750:Phosphoproteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012795:Sialoglycoproteins / Q000235:genetics*
D018076:DNA, Complementary; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D012313:RNA