title: TT virus: preferential distribution in CD19(+) peripheral blood mononuclear cells and lack of viral integration.
abstract: In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20% (25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19(+) cells. TTV DNA was also detected in CD3(+)/CD19(+) double-depleted cells but was undetectable in CD3(+) cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19(+) cells, CD3(+)-depleted, and CD3(+)/CD19(+) double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication.
authors.affiliation: Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, Hawaii, USA. qigui.yu@utoronto.ca
authors.forename: Qigui
authors.initials: Q
authors.lastname: Yu
authors.forename: Bruce
authors.initials: B
authors.lastname: Shiramizu
authors.forename: Vivek R
authors.initials: VR
authors.lastname: Nerurkar
authors.forename: Ningjie
authors.initials: N
authors.lastname: Hu
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Shikuma
authors.forename: Marian E
authors.initials: ME
authors.lastname: Melish
authors.forename: Karin
authors.initials: K
authors.lastname: Cascio
authors.forename: Allison
authors.initials: A
authors.lastname: Imrie
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
authors.forename: Richard
authors.initials: R
authors.lastname: Yanagihara
mesh_terms: D000328:Adult; D018941:Antigens, CD19 / Q000378:metabolism*; D001483:Base Sequence; D017252:CD3 Complex / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D015995:Prevalence; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016662:Virus Integration; D014779:Virus Replication
chemical_list: D018941:Antigens, CD19; D017252:CD3 Complex; D004279:DNA, Viral