[Determination of azoxystrobin in tea by HPLC].
A determination method has been developed for azoxystrobin in tea by HPLC. Azoxystrobin was extracted from a sample with acetone, and the extract was passed through an alumina column to remove tannin. The eluate was concentrated to ca. 25 mL and passed through a Sep-Pak Vac tC18 to remove pigments. The eluate was cleaned-up by using liquid-liquid partition, and Florisil and silica-gel columns. The HPLC analysis for azoxystrobin was carried out on a C18 column with acetonitrile-water (9:11) as the mobile phase, with ultraviolet detection at 260 nm. The recovery of azoxystrobin fortified at the level of 0.4 microgram/g was 90.2% and the limit of determination was 0.2 microgram/g.
Chonan
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Hokkaido Institute of Public Health: Nishi 12-chome, Kita 19-jo, Kita-ku, Sapporo 060-0819, Japan.
D000179:Acrylates / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008689:Methacrylates; D010573:Pesticide Residues / Q000032:analysis*; D011743:Pyrimidines / Q000032:analysis*; D000073739:Strobilurins; D013662:Tea / Q000737:chemistry*
D000179:Acrylates; D000935:Antifungal Agents; D008689:Methacrylates; D010573:Pesticide Residues; D011743:Pyrimidines; D000073739:Strobilurins; D013662:Tea; C087670:azoxystrobin