Expression of scavenger receptor for phosphatidylserine and oxidized lipoprotein (SR-PSOX) in human atheroma.
Recently, we identified a novel macrophage cell-surface receptor for oxidized low-density lipoprotein (Ox-LDL), designated SR-PSOX (scavenger receptor for phosphatidylserine and oxidized lipoprotein). Here we examine SR-PSOX expression in human atherosclerotic lesions using carotid endarterectomy specimens from 21 patients, directional coronary atherectomy specimens from 11 patients, and normal aortas from 2 patients. RT-PCR analysis demonstrated that SR-PSOX expression was upregulated in atherosclerotic lesions, but undetectable in normal aortas. Immunohistochemistry showed that SR-PSOX was abundantly expressed by macrophages in the intima of atherosclerotic lesions. Taken together, SR-PSOX may be involved in Ox-LDL uptake and subsequent foam cell transformation in macrophages in vivo and therefore may play important roles in human atherosclerotic lesion formation.
Minami
M
M
Departments of Geriatric Medicine, Graduate School of Medicine, Kyoto University, Japan.
Kume
N
N
Shimaoka
T
T
Kataoka
H
H
Hayashida
K
K
Yonehara
S
S
Kita
T
T
D001161:Arteriosclerosis / Q000235:genetics* / Q000378:metabolism; D016893:Carotid Stenosis / Q000235:genetics / Q000601:surgery; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC; D016894:Endarterectomy, Carotid; D005786:Gene Expression Regulation; D006801:Humans; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008264:Macrophages / Q000473:pathology / Q000502:physiology; D008565:Membrane Proteins; D010718:Phosphatidylserines / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000235:genetics*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051122:Scavenger Receptors, Class B
C423727:CXCL16 protein, human; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008565:Membrane Proteins; D010718:Phosphatidylserines; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C099252:oxidized low density lipoprotein; C108132:oxidized very low density lipoprotein