Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang).
The hypersensitive reaction (HR) in plants is typified by a rapid and localized cell death at the site of pathogen infection. To understand better the molecular and cellular defence mechanism controlling HR, hot pepper leaves (Capsicum annuum cv. Pukang) were inoculated with the soybean pustule pathogen Xanthomonas campestris pv. glycine 8ra. By using the DD-PCR technique, a cDNA fragment was identified that exhibited a sequence similarity to the recently identified tobacco pathogen-induced oxygenase (PIOX) with homology to animal cyclo-oxygenase (COX). Subsequently, the full-length cDNA clone, pCa-COX1, encoding the COX homologue from the pathogen-inoculated hot pepper leaf cDNA library was isolated. The deduced amino acid sequence of Ca-COX1 shares 85.8% identity with tobacco PIOX and displays a significant degree of sequence identity (21.7-23.7%) with mammalian COXs. The expression of Ca-COX1 was markedly induced at 4-12 h after pathogen infection, while HR cell death on pepper leaves appeared at approximately 15 h post-inoculation. These results are consistent with the notion that the lipid-derived signalling pathway is involved in the initial response of hot pepper plants to pathogen infection.
Kim
Young-Cheol
YC
Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusung, Taejon 305-600, Korea.
Yi
So-Young
SY
Mang
Hyung Gon
HG
Seo
Young Sam
YS
Kim
Woo Taek
WT
Choi
Doil
D
D000595:Amino Acid Sequence; D002212:Capsicum / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D051545:Cyclooxygenase 1; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics; D016959:Xanthomonas campestris / Q000254:growth & development
D018076:DNA, Complementary; D007527:Isoenzymes; D010940:Plant Proteins; D010105:Oxygenases; C115355:pathogen-induced oxygenase, Nicotiana tabacum; D051545:Cyclooxygenase 1; D011451:Prostaglandin-Endoperoxide Synthases