[Investigation of the polymorphism of HLA-DQCAR microsatellites in the healthy and pulmonary tuberculosis in Hunan Hans].
OBJECTIVE
To observe HLA-DQCAR microsatellite distribution and the association of this gene with pulmonary tuberculosis in Hunan Hans.


METHODS
The polymorphism of HLA-DQCAR microsatellite was analyzed by electrophoresis on a 8% denaturing polyacrylamide gel. The length of its alleles was determined by sequencing.


RESULTS
Ten alleles, DQCAR101, 103, 105, 107, 111, 113, 115, 117, 119 and 121, were found in 68 Hunan healthy Hans, but the frequencies of them were different. In addition to the above-mentioned alleles, DQCAR 99 and 109, were also found in 79 pulmonary tuberculosis patients in Hunan Hans. This results as well showed that DQCAR115 allele was significantly associated with pulmonary tuberculosis in Hunan Hans (RR = 2.12, Pc < 0.05).


CONCLUSIONS
The frequency of DQCAR alleles is of great polymorphism. DQCAR115 is significantly associated with pulmonary tuberculosis in Hunan Hans.
Jiang
C
C
Research Laboratory of Immunology, Hunan Medical University, Changsha 410078, China.
Guo
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D002681:China; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005192:Family Health; D005787:Gene Frequency; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000235:genetics*
D006683:HLA-DQ Antigens; D004247:DNA