Regulated gene expression in the chicken embryo by using replication-competent retroviral vectors.
Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo.
Sato
Noboru
N
Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. nsato@fmu.ac.jp
Matsuda
Kenji
K
Sakuma
Chie
C
Foster
Douglas N
DN
Oppenheim
Ronald W
RW
Yaginuma
Hiroyuki
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000502:physiology; D002642:Chick Embryo; D003587:Cytomegalovirus / Q000235:genetics; D004318:Doxycycline / Q000494:pharmacology; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D014779:Virus Replication
D000900:Anti-Bacterial Agents; D008164:Luminescent Proteins; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein; D049452:Green Fluorescent Proteins; D004318:Doxycycline