title: Cloning and identification of an angiostatic molecule IP10/crg -2.
abstract: AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2).METHODS:The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+).RESULTS:The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing.CONCLUSION:Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Yang
authors.forename: Hua-Zhang
authors.initials: HZ
authors.lastname: An
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Feng-Tian
authors.initials: FT
authors.lastname: He
authors.forename: Zhe-Yi
authors.initials: ZY
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Han-Ping
authors.initials: HP
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Xiao
authors.forename: Dai-Ming
authors.initials: DM
authors.lastname: Fan