Alcohol-water as a novel medium for beta-hematin preparation.
It is shown that alcohol-water mixtures (e.g., 43 wt% ethanol) provide a suitable medium for the efficient production in high yield of beta-hematin from ferriprotoporphyrin IX (FP), as measured by infrared spectroscopy. Previous and present light-absorption data of FP, obtained under specific acid conditions in an ethanol-water medium, suggest the presence of FP monomers, which are considered to promote the reaction leading to beta-hematin. Other aspects of the mechanism of reaction are discussed.
Blauer
G
G
Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
Akkawi
M
M
D000433:1-Propanol / Q000737:chemistry; D019840:2-Propanol / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry*; D002738:Chloroquine / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D006427:Hemin / Q000032:analysis / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D012118:Resorcinols / Q000737:chemistry; D013054:Spectrophotometry, Atomic; D013055:Spectrophotometry, Infrared; D013997:Time Factors; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
D000438:Alcohols; D010636:Phenols; D020033:Protein Isoforms; D012118:Resorcinols; D014867:Water; D000431:Ethanol; D006427:Hemin; D002738:Chloroquine; D000433:1-Propanol; D019840:2-Propanol; D000432:Methanol; C031389:resorcinol