Use of FTA gene guard filter paper for the storage and transportation of tumor cells for molecular testing.
BACKGROUND
Efficient methods of storing tumor specimens for molecular testing are needed in the modern surgical pathology laboratory. The FTA Gene Guard system is a novel method for the collection and room temperature storage of blood samples for DNA testing. The method uses index card-sized filter papers that provide an ideal medium on which to store tumor specimens for DNA testing.


OBJECTIVE
To determine whether FTA filter paper can be used in the surgical pathology laboratory to store tumor cells for DNA testing.


METHODS
Cell suspensions were prepared from 60 surgical specimens, and DNA was extracted either immediately or after storage on FTA paper. The DNA extracted by each method was tested by polymerase chain reaction (PCR) for the beta-globin and interferon gamma genes, and the results were compared. Fifteen lymph node specimens stored on FTA paper were then tested for immunoglobulin heavy chain (IgH) gene rearrangement by PCR, and these results were compared with those obtained for immediately extracted DNA.


METHODS
University medical center.


RESULTS
The DNA extracted from cells stored on FTA paper performed as well in the PCR as the freshly extracted DNA in nearly all cases (>95%). The results of tests for IgH gene rearrangements showed 100% concordance between the 2 methods of DNA extraction.Conclusion.-Cells from surgical specimens can be stored on FTA paper for extended lengths of time, and DNA can be extracted from these cells for PCR-based testing. FTA filter paper is a reliable medium for the storage and/or transport of tumor cells for PCR-based DNA analysis.
Dobbs
Larry J
LJ
Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA. Ldobbs@pcmh.com
Madigan
Merle N
MN
Carter
Alexis B
AB
Earls
Lori
L
D003581:Cytodiagnosis / Q000295:instrumentation*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006801:Humans; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D010209:Paper; D010340:Pathology, Surgical / Q000379:methods*; D016133:Polymerase Chain Reaction; D013048:Specimen Handling / Q000295:instrumentation*
D004247:DNA