title: Plasmodium vivax Duffy binding protein peptides specifically bind to reticulocytes.
abstract: Plasmodium vivax Duffy Binding Protein (Pv-DBP) is essential during merozoite invasion of reticulocytes. Reticulocyte binding region identification is important for understanding Pv-DBP reticulocyte recognition. Fifty 20 mer non-overlapping peptides, spanning Pv-DBP sequences, were tested in erythrocyte and reticulocyte binding assays. Ten HARBPs, mainly located in region II (Kd 50-130 nM), were High Activity Reticulocyte Binding Peptides (HARBPs); one bound to erythrocytes. Reticulocyte trypsin-, chymotrypsin- or neuraminidase- treatment affects HARBP binding differently, suggesting that these peptides have different reticulocyte-binding-sites. Some peptides bound to a Coomasie non-stainable 40 Kda band. Some HARBPs were able to block recombinant PvRII binding (Pv-DBP region II) to Duffy positive reticulocytes.
authors.affiliation: Fundacion Instituto de Inmunología de Colombia Carrera SO No. 26-00, Bogotá, Colombia. marisolocampo@altavista.com
authors.forename: Marisol
authors.initials: M
authors.lastname: Ocampo
authors.forename: Ricardo
authors.initials: R
authors.lastname: Vera
authors.forename: Luis
authors.initials: L
authors.lastname: Eduardo Rodriguez
authors.forename: Hernando
authors.initials: H
authors.lastname: Curtidor
authors.forename: Mauricio
authors.initials: M
authors.lastname: Urquiza
authors.forename: Jorge
authors.initials: J
authors.lastname: Suarez
authors.forename: Javier
authors.initials: J
authors.lastname: Garcia
authors.forename: Alvaro
authors.initials: A
authors.lastname: Puentes
authors.forename: Ramsés
authors.initials: R
authors.lastname: Lopez
authors.forename: Mary
authors.initials: M
authors.lastname: Trujillo
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Torres
authors.forename: Manuel Elkin
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D001665:Binding Sites; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry*; D010966:Plasmodium vivax / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism*; D017086:beta-Thalassemia / Q000097:blood
chemical_list: D000953:Antigens, Protozoan; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; C066001:Duffy antigen binding protein, Plasmodium; D008024:Ligands; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins