Isolation and characterization of the muscle-specific isoform of creatine kinase from the zebrafish, Danio rerio.
We have isolated and characterized a cDNA sequence corresponding to the zebrafish muscle-specific isoform of creatine kinase. The sequence is 1552 bases in length and contains an open reading frame capable of producing a 381 amino acid protein. The sequence is very similar to muscle-specific creatine kinases isolated from other species at both the nucleotide and amino acid levels but contains some differences from a previously reported zebrafish clone.
Harder
G
G
Department of Zoology, University of Manitoba, Winnipeg, Canada.
McGowan
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003402:Creatine Kinase / Q000302:isolation & purification* / Q000378:metabolism; D018076:DNA, Complementary; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009132:Muscles; D009928:Organ Specificity; D015027:Zebrafish / Q000378:metabolism*
D018076:DNA, Complementary; D007527:Isoenzymes; D003402:Creatine Kinase