title: Headspace sorptive extraction (HSSE), stir bar sorptive extraction (SBSE), and solid phase microextraction (SPME) applied to the analysis of roasted Arabica coffee and coffee brew.
abstract: Headspace sorptive extraction (HSSE) and stir bar sorptive extraction (SBSE), two recently introduced solventless enrichment techniques, have been applied to the analysis of the headspace of Arabica roasted coffee and of the headspace of the brew and of the brew itself. In both HSSE and SBSE enrichment is performed on a thick film of poly(dimethylsiloxane) (PDMS) coated onto a magnet incorporated in a glass jacket. Sampling is done by placing the PDMS stir bar in the headspace (gas phase extraction or HSSE) or by immersing it in the liquid (liquid phase extraction or SBSE). The stir bar is then thermally desorbed on-line with capillary GC-MS. The performance of HSSE and SBSE have been compared through the determination of the recoveries and relative abundances of 16 components of the coffee volatile fraction to classical static headspace (S-HS) and to headspace and in-sample solid phase microextraction (HS-SPME and IS-SPME, respectively) applying the fibers PDMS 100 microm, Carbowax/divinylbenzene 65 microm (CW/DVB), Carboxen/PDMS 75 microm(CAR/PDMS), polyacrylate 85 microm(PA), PDMS/divinylbenzene 65 microm(PDMS/DVB), and Carboxen/divinylbenzene/PDMS 50-30 microm(CAR/PDMS/DVB). In all cases, HSSE and SBSE gave higher recoveries, and this is entirely due to the high amount of PDMS applied.
authors.affiliation: Dipartimento di Scienza e Tecnologia del Farmaco, Via Pietro Giuria 9, I-10125 Torino, Italy. bicchi@pharm.unito.it
authors.forename: Carlo
authors.initials: C
authors.lastname: Bicchi
authors.forename: Cristina
authors.initials: C
authors.lastname: Iori
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rubiolo
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003069:Coffee