A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'.
A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5'-RTGC/GCAY-3' and cleaves it in the center generating blunt-ended DNA fragments.
Kesminiene
A
A
MBI Fermentas, and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
Maneliene
Z
Z
Vitkute
J
J
Petrusyte
M
M
Janulaitis
A
A
D001419:Bacteria / Q000201:enzymology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
D009841:Oligonucleotides; D004247:DNA; C452019:endodeoxyribonuclease FspAI; D015252:Deoxyribonucleases, Type II Site-Specific