title: Maintenance and characterization of an Epstein Barr virus-infected CD56-negative T cell lymphoma.
abstract: T cell lymphoma carrying Epstein Barr virus (EBV(+) TL) is very rare among Western countries while it is much more common among Japanese. Here we report an EBV(+) TL which has been maintained for years by the use of mice with severe combined immune deficiency (SCID) mice. Lymphoma was obtained from a 55-year-old male suffering from oculomotor nerve palsy and lymphadenopathy. A small piece of biopsied tumor was transplanted into SCID mice and the lymphoma has been maintained for over 3 years with passages every 2 - 3 weeks. The maintained lymphoma, termed as TMS24, and the original lymphoma cells showed identical phenotype and genotype, including diffuse medium-sized cell morphology lacking granules, suppressor / cytotoxic immunophenotype and identical T cell receptor beta-chain gene rearrangement mode. Further, both were shown to carry an identical EBV clone in terms of the number of terminal repeats and the latency II-type restricted gene expression profile. Cytogenetically, TMS24 retained two characteristic chromosomal translocations of t(1;18)(q32;q21) and t(6;12)(p21;q24). Since only one cell line with such characters has been reported previously, TMS24 should be useful for detailed analysis of EBV(+) TL.
authors.affiliation: Division of Pathology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. izm9182@ims.u-tokyo.ac.jp
authors.forename: Izuru
authors.initials: I
authors.lastname: Maekawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Naoto
authors.initials: N
authors.lastname: Aoki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Morishita
authors.forename: Norifumi
authors.initials: N
authors.lastname: Tsukamoto
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Karasawa
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Nonaka
authors.forename: Mami
authors.initials: M
authors.lastname: Shiota
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nojima
authors.forename: Shigeo
authors.initials: S
authors.lastname: Mori
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D015153:Blotting, Western; D019002:CD56 Antigen / Q000378:metabolism*; D002460:Cell Line / Q000166:cytology*; D002999:Clone Cells / Q000166:cytology*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D016130:Immunophenotyping; D017403:In Situ Hybridization; D007621:Karyotyping; D016399:Lymphoma, T-Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008854:Microscopy, Electron; D008875:Middle Aged; D009368:Neoplasm Transplantation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000502:physiology; D014178:Translocation, Genetic; D014764:Viral Proteins / Q000032:analysis
chemical_list: D019002:CD56 Antigen; D014764:Viral Proteins