[The effects of different glucose feeding modes on hEGF production in an excretory recombinant Escherichia coli K12 system].
The effects of different glucose feeding modes on hEGF expression were evaluated in an excretory recombinant E. coli K12 system. The results showed that, compared with batch cultivation, the plasmid stability and density of plasmid-retaining cells were improved by all three glucose feeding modes (intermittent, pH-stat and constant-rate). It was shown that hEGF yields were improved up to 25.5% and 28.1% by intermittent or pH-stat glucose feeding respectively. Especially, up to 150% improvement of hEGF production was achieved by constant feeding of 200 g/L glucose solution at a rate of 0.11 mL/min. The effects of further combined feeding with other medium components (ampicillin, nitrogen sources, and inorganic salts) and inducer on hEGF yield were also examined in the bench-top fermentor.
Xu
Z N
ZN
Dept. of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China. xuzn@mail.hz.zj.cn
Cen
P L
PL
Wong
W K
WK
D001431:Bacteriological Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D003470:Culture Media / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D005947:Glucose