Metallothionein-bound cadmium in the gut of the insect Orchesella cincta (Collembola) in relation to dietary cadmium exposure.
Metallothionein is considered to be a potential biomarker for heavy metal exposure in the terrestrial environment. However, limited information is available on metallothioneins from insects, a major class of terrestrial invertebrates. In this study we have quantified metallothioneins in the springtail Orchesella cincta by determining metallothionein-bound cadmium after separation of these proteins using gel filtration and reversed phase chromatography from total body homogenates of animals dietary exposed to different concentrations of cadmium. Furthermore, we have studied in more detail where cadmium and metallothionein-bound cadmium is located within this animal. The concentration of metallothionein-bound cadmium increases with the exposure concentration in the same way as the total internal concentration. Both reach a plateau at an exposure concentration of approximately 1.0 micromol Cd/dry food. Cadmium is primarily located within the gut of O. cincta and isolation of metallothionein from this organ gives results identical to isolations from total bodies. Based on this results an estimation of the metallothionein level at the highest exposure concentration results in a concentration of about 115 microg metallothionein/g fresh gut. The O. cincta metallothionein gives the possibility of using this protein as a biomarker for heavy metal exposure in soil insects.
Hensbergen
P J
PJ
Department of Ecology and Ecotoxicology, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, Netherlands. paulh@bio.vu.nl
van Velzen
M J
MJ
Nugroho
R A
RA
Donker
M H
MH
van Straalen
N M
NM
D000818:Animals; D001181:Arthropods / Q000378:metabolism*; D015415:Biomarkers; D002104:Cadmium / Q000008:administration & dosage / Q000378:metabolism*; D002850:Chromatography, Gel; D004032:Diet; D004064:Digestive System / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism*; D008670:Metals / Q000378:metabolism; D011485:Protein Binding; D012989:Soil Pollutants / Q000378:metabolism
D015415:Biomarkers; D008670:Metals; D012989:Soil Pollutants; D002104:Cadmium; D008668:Metallothionein