Effects of polysaccharides from rhizomes of Curcuma zedoaria on macrophage functions.
The effects of Curcuma zedoaria, which is used as a condiment, in perfumery, and as a medicine, on immune response were investigated by measuring macrophage-stimulating activity in macrophages and RAW 264.7 cells. In this study, CZ-1 and CZ-1-III, the fractions partially purified from C. zedoaria, had a strong, dose-dependent lysosomal enzyme activity. It was suggested that active portions of CZ-1-III were polysaccharides rather than proteins. Phagocytic activity increased as a similar pattern in both the gram-negative and gram-positive bacteria, time-dependently. It was demonstrated that CZ-1-III can augment the oxygen burst response but had an even higher activity in vivo than in vitro. Also a significant increase of H2O2, NO, and TNF-alpha production was observed. However, the production of TNF-alpha at the concentration of 1,000 microg/ml decreased. These data suggested that C. zedoaria had macrophage-stimulating activity and the possibility of being used as a biological response modifier.
Kim
K I
KI
Graduate School of Biotechnology, Korea University, Seoul.
Shin
K S
KS
Jun
W J
WJ
Hong
B S
BS
Shin
D H
DH
Cho
H Y
HY
Chang
H I
HI
Yoo
S M
SM
Yang
H C
HC
D000818:Animals; D002460:Cell Line; D030024:Curcuma / Q000737:chemistry*; D004926:Escherichia coli / Q000276:immunology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology; D066298:In Vitro Techniques; D008247:Lysosomes / Q000201:enzymology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009573:Nitrites / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects; D027343:Rhizome / Q000737:chemistry; D013211:Staphylococcus aureus / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007155:Immunologic Factors; D009573:Nitrites; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D006861:Hydrogen Peroxide