[Biocompatibility of HA/TCP biphasic ceramics with co-cultured human osteoblasts in vitro].
The biocompatibility of HA/TCP ceramic was evaluated by investigation of attachment and growth of osteoblasts on biomaterial, as well as monitoring the effects of biomaterial on expression of functional phenotypes of co-cultured osteoblasts in vitro. When co-cultured with HA/TCP ceramics, osteoblasts firstly attached to the surface of HA/TCP disk, then attached to notches and grew into the micropores of biomaterial during further culture period. At last, the ceramics were almost packed with osteoblasts. Additionally, osteoblasts co-cultured with HA/TCP were similar to osteoblasts cultured under normal condition in osteoblastic phenotypes; the secreted lots of collagen type I, possess strong activity of Alkaline Phosphatase and mineralized extracellular matrix. The fact that osteoblasts could grow well on HA/TCP ceramics and the biomaterial did not affect their physiological function suggest that HA/TCP ceramic is biocompatible with human osteoblasts.
Lu
X
X
Laboratory of Transplant Engineering and Immunology, West China Hospital of Sichuan University, Chengdu 610041.
Li
S
S
Zhang
J
J
Zhang
Z
Z
Lu
B
B
Bu
H
H
Li
Y
Y
Cheng
J
J
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002455:Cell Division; D002478:Cells, Cultured; D002516:Ceramics; D017886:Durapatite; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite; C018392:tricalcium phosphate