title: Confocal laser scanning, conventional scanning and transmission electron microscopy of vertebrate cerebellar granule cells.
abstract: Confocal laser scanning microscopy of hamster cerebellar granular layer showed in montages of z-series the presence of small, medium and large granule cells. A granule cell Golgi cell ratio of 50/4 was observed surrounding glomerular regions. Field emission high resolution scanning electron microscopy of mouse cerebellar granular and molecular layers showed SE-I images of the outer and inner surfaces of nuclear and cytoplasmic compartments of chromium coated granule cells and the axo-spinodendritic synapses of parallel fibers with Purkinje cell dendrites. Conventional scanning electron microscopy of teleost fish cerebellar cortex showed three dimensional morphology of granule cell soma and processes and the synaptic relationship with mossy and climbing fibers, Golgi cell axonal ramifications and dendrites of stellate neurons, by means of SE-II and SE-III signal image mode, in sagittally and transversally cryofractured cerebellar cortex. SE-II images of the non-synaptic segments and synaptic varicosities of parallel fiber outer surface were characterized in the molecular layer. Ultrathin sections of transmission electron microscopy (TEM) revealed somato-somatic, dendro-somatic and dendro-dendritic like-desmosomal and like-hemidesmosomal junctions in human cerebellar granule cells. Freeze-etching replicas of mouse cerebellar cortex displayed granule cell intramembrane morphology, cytoplasmic fractured face and the Bergman glial cell cytoplasm completely surrounding the parallel fibers in the molecular layer. The mossy fiber-granule cell dendrite synaptic relationship was observed in sagittally and transversally cryofractured cerebellar cortex and correlated with TEM images. SE-II images of the climbing fiber synaptic connections with granule cell dendrites were obtained in teleost fish cerebellar cortex. One to one axo-dendritic synaptic contacts between Golgi cell axonal ramifications and granule cell dendrites were also seen. The above findings provide new vistas for future studies dealing with intracortical circuits and information processing in the cerebellar cortex.
authors.affiliation: Instituto de Investigaciones Biológicas, Facultad de Medicina, Universidad del Zulia, Apartado Postal No 526, Maracaibo, Venezuela. ocastejo@cantv.net
authors.forename: O J
authors.initials: OJ
authors.lastname: Castejón
authors.forename: H V
authors.initials: HV
authors.lastname: Castejón
authors.forename: R P
authors.initials: RP
authors.lastname: Apkarian
mesh_terms: D000818:Animals; D002397:Catfishes; D002531:Cerebellum / Q000166:cytology*; D006224:Cricetinae; D005260:Female; D005613:Freeze Etching; D005614:Freeze Fracturing; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*