title: Bcr in vascular smooth muscle cells involvement of Ras and Raf-1 activation by Bcr.
abstract: Bcr gene was originally identified by its presence in the chimeric Bcr/Abl oncogene. In vascular smooth muscle cells, platelet-derived growth factor-BB (PDGF) stimulated Bcr kinase activity. The mutant PDGF receptor for PI3-K, but not for PLC-gamma binding sites, showed significantly reduced Bcr kinase activity. Bcr wild-type enhanced, whereas Bcr kinase negative form inhibited PDGF-stimulated ERK1/2 activity. A dominant negative Ras did not inhibit Bcr kinase activation, and overexpression of Bcr increased Ras/Raf-1 activity and DNA synthesis. These results demonstrated the importance of Bcr in PDGF-mediated events such as activation of Ras, Raf-1, and ERK1/2 and stimulation of DNA synthesis.
authors.affiliation: Center for Cardiovascular Research, University of Rochester, New York 14642, USA. jun-ichi_abe@urmc.rochester.edu
authors.forename: J I
authors.initials: JI
authors.lastname: Abe
authors.forename: W
authors.initials: W
authors.lastname: Che
authors.forename: M
authors.initials: M
authors.lastname: Yoshizumi
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Glassman
authors.forename: S
authors.initials: S
authors.lastname: Ohta
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Arlinghaus
authors.forename: B C
authors.initials: BC
authors.lastname: Berk
mesh_terms: D000818:Animals; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D015513:Oncogene Proteins / Q000502:physiology*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology; D018631:ras Proteins / Q000235:genetics*
chemical_list: D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D051562:Proto-Oncogene Proteins c-bcr; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins