title: Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen.
abstract: In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.
authors.affiliation: Biomedical Research and Study Centre, University of Latvia, Ratsupites Str. 1, LV-1067, Riga, Latvia. irina@biomed.lu.lv
authors.forename: Irina
authors.initials: I
authors.lastname: Sominskaya
authors.forename: Wilma
authors.initials: W
authors.lastname: Paulij
authors.forename: Juris
authors.initials: J
authors.lastname: Jansons
authors.forename: Dirk
authors.initials: D
authors.lastname: Sobotta
authors.forename: Dzidra
authors.initials: D
authors.lastname: Dreilina
authors.forename: Cecile
authors.initials: C
authors.lastname: Sunnen
authors.forename: Helga
authors.initials: H
authors.lastname: Meisel
authors.forename: Wolfram H
authors.initials: WH
authors.lastname: Gerlich
authors.forename: Paul
authors.initials: P
authors.lastname: Pumpens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050807:presurface protein 2, hepatitis B surface antigen