Phenols and phenol oxidases are involved in cadmium accumulation in the water plants Nymphoides peltata (Menyanthaceae) and Nymphaeae (Nymphaeaceae).
This comparative study investigates the mechanism of cadmium accumulation in the semiaquatic plant Nymphoides peltata (Menyanthaceae) and the aquatic plant Nymphaea (Nymphaeaceae). It was conducted as part of an ongoing study of the use of water plants for phytoremediation. Epidermal structures, known as hydropotes, are located on the abaxial epidermis of the leaf laminae of Nymphoides peltata and are shown to contain phenols, peroxidase and polyphenol oxidase activities. When plants are subjected to 50 mg/l of cadmium in the growth medium, these hydropotes accumulate cadmium. Cadmium-induced increases in phenols, peroxidase and polyphenol oxidase activities were determined in plant extracts. Cadmium binding by polymerized phenols was demonstrated in vivo. In comparison with Nymphaeae epidermal glands, N. peltata hydropotes are larger, open, and create bigger crystal, the latter principally composed of calcium and, proportionally, less cadmium. Although both plants showed similar levels of cadmium accumulation, N. peltata was sensitive while Nymphaeae was resistant to this cadmium level. It is suggested that in these water plants the main mechanism for cadmium accumulation is based on the trapping of cadmium crystals by polymerized phenols in specialized epidermal structures and this is due to peroxidase and polyphenol oxidase activities. Nymphaeae, with greater peroxidase activity and more polyphenols, is more resistant to this heavy metal than N. peltata.
Lavid
N
N
Department of Agricultural Botany, The Hebrew University of Jerusalem, Rehovot, Israel. telor@agri.huji.ac.il
Schwartz
A
A
Lewinsohn
E
E
Tel-Or
E
E
D001673:Biodegradation, Environmental; D002104:Cadmium / Q000008:administration & dosage / Q000378:metabolism*; D003460:Crystallization; D007150:Immunohistochemistry; D019684:Magnoliopsida / Q000201:enzymology / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000096:biosynthesis*; D030721:Nymphaea / Q000201:enzymology / Q000378:metabolism*; D009195:Peroxidase / Q000096:biosynthesis*; D010636:Phenols / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology; D018514:Plant Structures / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000378:metabolism
D010636:Phenols; D014874:Water Pollutants, Chemical; D002104:Cadmium; D009195:Peroxidase; D014442:Monophenol Monooxygenase