[Comparison of HLA class I typing by serology with DNA typing].
OBJECTIVE
To compare HLA class I typing by serology with PCR-SSP method in the Chinese population.


METHODS
HLA-A and -B antigens were typed by serology with microlymphocytotoxicity and DNA typing with PCR-SSP in 525 clinical samples. Reliability, reproducibility and clinical practicability of both methods were compared according to the typing results.


RESULTS
Serological typing for HLA class I was rapid and simple, costing 3 hours. The discrepancy rate between serology and PCR-SSP for HLA-A antigen was 9.0%, consisting of 21 antigens being incorrectly interpreted by serology and 26 of serological "blanks" turning out to be definable alleles by DNA typing. The discrepancy rate for HLA-B antigen was 12.2%, containing 39 antigens being incorrectly interpreted and 35 of serological "blanks" turning out to be definable alleles by PCR-SSP typing. DNA typing for HLA class I by PCR-SSP proved to be an accurate, reliable and well-reproducible technique within 5 hours. While it was difficult to suit clinical assay by a large scale screening because of complicated operation and high technical condition.


CONCLUSIONS
DNA typing for HLA class I by PCR-SSP is suitable for clinical application in the Chinese population with a higher precision than serology. Screening test by serology is recommended. Ambiguous or blank antigens by serology should be retyped by DNA typing.
Tan
J
J
Renal Transplant Center, Fuzhou General Hospital, Fuzhou 350025, China.
Tang
X
X
Xie
T
T
D000293:Adolescent; D000328:Adult; D004247:DNA / Q000032:analysis; D004311:Double-Blind Method; D005260:Female; D015234:HLA-A Antigens / Q000145:classification* / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D012698:Serologic Tests
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D004247:DNA