Crystallization and preliminary crystallographic analysis of a family 45 endoglucanase from the thermophilic fungus Melanocarpus albomyces.
20K-cellulase, an endoglucanase produced by the thermophilic fungus Melanocarpus albomyces, has been crystallized. It belongs to the family 45 of glycoside hydrolases and has sequence homology with Humicola insolens endoglucanase V (EGV). However, in contrast to EGV, it does not harbour a cellulose-binding module. Optimization of the crystallization conditions using a PEG/ion combination and microseeding techniques was employed to improve the quality and the size of the crystals. A complete data set to 2.2 A resolution was collected using synchrotron radiation. Preliminary crystallographic analysis showed that the crystals belong to the tetragonal space group P4(1)2(1)2/P4(3)2(1)2, with unit-cell parameters a = 47.3, b = 47.3, c = 177.3 A and one molecule per asymmetric unit.
Hirvonen
Mika
M
Turku Centre for Biotechnology, University of Turku and Abo Akademi University, PO Box 123, Turku 20521, Finland.
Papageorgiou
Anastassios C
AC
D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D005658:Fungi / Q000201:enzymology*; D011487:Protein Conformation
D002480:Cellulase