title: Trial of autotransplantation of cryopreserved thyroid tissue for postoperative hypothyroidism in patients with Graves' disease.
abstract: BACKGROUND
  Some patients with Graves' disease who select surgical therapy so they can discontinue antithyroid medication require lifelong levo-thyroxin (l-T4) replacement therapy because of irreversible postoperative hypothyroidism. The aim of this study was to enable the replacement of absent thyroid hormone through autotransplanted thyroid tissue that had been cryopreserved since the initial thyroid operation, and to release these patients from lifelong l-T4 administration.
  
  
  METHODS
  At the time of subtotal thyroidectomy for Graves' disease, the surgical specimen was partially cryopreserved at -196 degrees C until it was used for autotransplantation. After obtaining sufficient informed consent, four patients with postoperative hypothyroidism underwent autotransplantation of cryopreserved thyroid tissues. These patients required 50 to 150 microg/day of l-T4 at 1.8, 3.4, 3.5, and 2.8 years after operation. For the transplantation, 2.5 to 3.5 g of cryopreserved thyroid tissue was autotransplanted into the forearm muscle of each patient.
  
  
  RESULTS
  In three of the patients, l-T4 administration could be discontinued and the clinical symptoms of hypothyroidism disappeared because of an improved serum thyroid-stimulating hormone level. Pathologic and immunohistochemical examinations of the thawed cryopreserved tissue demonstrated well-preserved thyroid structure and thyroglobulin-positive follicular cells and colloids, suggesting that the transplanted material was functional. In addition, 123I scintiscanning in patients 1 and 2 indicated an accumulation of radioactive iodine at the transplantation sites. One patient, who was able to discontinue l-T4 administration for 6 months, subsequently required l-T4 again because of recurrent hypothyroidism.
  
  
  BACKGROUND
  Despite a few remaining uncertainties that must be resolved before this procedure is optimized, autotransplantation of cryopreserved thyroid tissue promises to be a useful therapeutic procedure for treating permanent postoperative hypothyroidism in patients with Graves' disease.
authors.affiliation: Nippon Medical School, Department of Surgery II, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shimizu
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Kumita
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Mitsuji
authors.initials: M
authors.lastname: Nagahama
authors.forename: Wataru
authors.initials: W
authors.lastname: Kitagawa
authors.forename: Haruki
authors.initials: H
authors.lastname: Akasu
authors.forename: Takashi
authors.initials: T
authors.lastname: Oshina
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kumasaki
authors.forename: Shigeo
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D000906:Antibodies / Q000032:analysis; D015925:Cryopreservation; D006111:Graves Disease / Q000601:surgery*; D006801:Humans; D007037:Hypothyroidism / Q000175:diagnosis / Q000000981:diagnostic imaging / Q000209:etiology / Q000601:surgery*; D007453:Iodide Peroxidase / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011877:Radionuclide Imaging; D011989:Receptors, Thyrotropin / Q000276:immunology; D013954:Thyroglobulin / Q000097:blood; D013961:Thyroid Gland / Q000737:chemistry / Q000000981:diagnostic imaging / Q000637:transplantation*; D013965:Thyroidectomy / Q000009:adverse effects*; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D014021:Tissue Preservation; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic; D014284:Triiodothyronine / Q000097:blood
chemical_list: D000906:Antibodies; D011989:Receptors, Thyrotropin; D014284:Triiodothyronine; D013972:Thyrotropin; D013954:Thyroglobulin; D007453:Iodide Peroxidase; D013974:Thyroxine