title: Complete nucleotide sequence and the genome organization of Patchouli mild mosaic virus RNA1.
abstract: The nucleotide sequence of the RNA1 of Patchouli mild mosaic virus (PatMMV) has been determined. It contains 5,957 nucleotides excluding the 3'-terminal poly(A) tail and contains a single long open reading frame (ORF) of 5,613 nucleotides extending from nucleotide 235 to 5847. The predicted polyprotein encoded by the long ORF is 1,870 amino acids in length with a molecular weight of 210 kD. The conserved residues of RNA-dependent RNA polymerase, cysteine protease, purine NTP-binding domain and a cofactor for viral protease are present in a 210-kD polyprotein. As PatMMV RNA showed high sequence identity (81-97%) with BBWV-2 RNA, PaMMV may be one strain of BBWV-2.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan. ikegami@nodai.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: Y
authors.initials: Y
authors.lastname: Onobori
authors.forename: N
authors.initials: N
authors.lastname: Sugimura
authors.forename: T
authors.initials: T
authors.lastname: Natsuaki
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D019181:Fabavirus / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011061:Poly A; D012367:RNA, Viral / Q000032:analysis*; D017423:Sequence Analysis, RNA
chemical_list: D020121:5' Untranslated Regions; D012367:RNA, Viral; D011061:Poly A