Iron lactate-induced osteopenia in male Sprague-Dawley rats.
Osteopenia was induced in rats fed a diet containing 50,000 ppm (5%) iron lactate for 2 or 4 weeks. Blood chemistry, urinalysis, and bone histomorphometry of the proximal tibial metaphysis were performed. Urinary pyridinoline and deoxypyridinoline and the osteoclast number per bone surface were selected for the measurement of dynamic resorption. The osteoclast surface, eroded surface, and osteoblast surface increased at both ends of the exposure periods, and bone resorption and formation both increased. The bone volume, trabecular thickness, and trabecular number decreased, and the secondary spongiosa of proximal metaphysis showed a marked bone loss. However, no mineralization defect was observed. At the end of the 2-week exposure period, biomarkers of osteoclasts and osteoblasts had increased the most, and the osteoblast surface, osteoclast surface, and osteoclast number per bone surface increased with prolonged exposure. The pathological changes of the bone lesion in iron lactate-overloaded rats were similar to those in rats of the osteoporotic model, because they consisted of changes reflecting the increase of bone resorption and formation without an osteomalacic change. However, the decline of serum parathyroid hormone (PTH) levels was different from that of the osteoporosis model rat. We concluded iron-induced bone lesions probably differ from those of low turnover bone diseases.
Matsushima
S
S
Pathology Section, Drug Safety, Evaluation, Developmental Research Laboratories, Shionogi & Co, Ltd, Toyonaka, Osaka, Japan. shuuichi.matsushima@shionogi.co.jp
Hoshimoto
M
M
Torii
M
M
Ozaki
K
K
Narama
I
I
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000139:chemically induced* / Q000473:pathology; D005296:Ferrous Compounds / Q000633:toxicity*; D007773:Lactates / Q000633:toxicity*; D008297:Male; D010010:Osteoclasts / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000187:drug effects* / Q000473:pathology
D005296:Ferrous Compounds; D007773:Lactates; C101012:ferrous lactate