title: [Mycoplasma infection in human gastriointestinal carcinoma tissues].
abstract: OBJECTIVE
  To explore the association between the carcinoma and mycoplasma infection by immunohistochemistry.
  
  
  METHODS
  Antigen of anti-tumor monoclonal antibody PD4 was identified as mycoplasma hyorhinis. Using immunohistochemistry to study archived paraffin-embedded gastriointestinal tract carcinomas tissue. The PCR was applied to amplify the mycoplasma DNA for confirming immunohistochemistry results.
  
  
  RESULTS
  It was showed that mycoplasma was present in 56% (50/90 cases) of gastric carcinoma. The cancer tissues with high differentiation had a higher mycoplasma infection ratio than that of low differentiation cancer tissues. (P < 0.05). In control cases, mycoplasma infection was 28% (18/49) in chronic superficial gastritis, 30% (14/46) in gastric ulcer and 37% (18/49) in intestinal metaplasia of the stomach. Mycoplasma infection was 55.1% (32/58) in colon carcinoma and 20.9% (10/49) in adenomarous polyp (P < 0.005). It seems that colon carcinoma tissues with slight pathological grade had a higher percent of mycoplasma infection than that of cancer tissues with moderate and heavy pathological grade (P < 0.05).
  
  
  CONCLUSIONS
  The high infection of mycoplasmas in carcinoma tissues suggest an association between mycoplasma and cancer. The mechanism involved in oncogenesis by mycoplasmas remains to be elucidated.
authors.affiliation: BeiJing Institute for Cancer Research, Peking University School of Oncology, 100034, China.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Shou
authors.forename: J
authors.initials: J
authors.lastname: Wu
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004064:Digestive System / Q000382:microbiology / Q000473:pathology; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009174:Mycoplasma / Q000235:genetics / Q000378:metabolism; D009175:Mycoplasma Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial