Mouthrinses: a comparative microbiological study.
This study was performed in order to evaluate the efficacy of different mouthrinses whose use is extended in Spain. Six different antiseptic mouthrinses were studied by means of determination of Minimal Inhibitory Concentration (MIC) values against Klebsiella pneumoniae, Serratia marcescens, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium, Bacillus subtilis, Streptococcus mutans, Prevotella intermedia, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. Also in vivo experiments were carried out in volunteers by the use of mouthrinses and evaluation of bacterial populations before and after the treatment. Finally, the kinetics of bacterial death was determined. Results suggested that the determination of MIC values is not a reliable method to evaluate the antibacterial effect of such products. On the other hand those rinsing solutions based on the effect of oxygen, such as those containing carbamide peroxide have a greater efficacy against anaerobic bacteria compared with rinses whose active molecule is a disinfectant. Finally, the kinetics of bacterial death demonstrates that the essential oil rinse kills bacteria much faster. All tested mouthrinses were active as antibacterial although those based on oxygen production or essential oils were more active than solutions based on chlorhexidine and Triclosan.
Gautier
G
G
Unitat de Microbiologia, Dental School-Campus de Bellvitge, Universitat de Barcelona, Spain.
Noguer
M
M
Costa
N
N
Canela
J
J
Viñas
M
M
D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects*; D001421:Bacteria, Anaerobic / Q000187:drug effects; D000077463:Carbamide Peroxide; D002710:Chlorhexidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015169:Colony Count, Microbial; D004338:Drug Combinations; D004926:Escherichia coli / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009067:Mouthwashes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000187:drug effects; D018713:Prevotella intermedia / Q000187:drug effects; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000187:drug effects; D012706:Serratia marcescens / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects; D014260:Triclosan / Q000494:pharmacology; D014508:Urea / Q000031:analogs & derivatives* / Q000494:pharmacology
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D009067:Mouthwashes; D009822:Oils, Volatile; D016877:Oxidants; D010545:Peroxides; D000077463:Carbamide Peroxide; D000431:Ethanol; D014260:Triclosan; D014508:Urea; C010882:chlorhexidine gluconate; D002710:Chlorhexidine