Mechanism of AVP release and synthesis in chronic salt-loaded rats.
Arginine vasopressin (AVP) is involved in osmotic regulation in the brain and peripheral tissues. To elucidate the regulatory mechanism that involves AVP release in hyperosmolality, we investigated the regulation of the synthesis and release of AVP in chronic salt-loaded rats. In chronic salt-loaded rats, which were generated by free access to water containing 2% NaCl for 7 days, plasma osmolality was significantly increased compared with control value. When tested, the AVP content was significantly higher in plasma but lower in the pituitary and whole brain (hypothalamus, cortex and striatum) than in control rats. The expression of AVP mRNA in the brain was significantly up-regulated compared with that in control rats. These data lead to the suggestion that hyperosmolality stimulates AVP release from the brain and subsequently induces AVP synthesis in the brain. On the other hand, mRNA levels of vasopressin V1a receptor (V1aR), whose down-regulation is known to be a counteraction to the V1aR activation, was not changed in the brain, suggesting that the AVP seems not to interact with the V1aR in the brain. These results suggest that hyperosmosis promotes the release of AVP into plasma, the subsequent induction of AVP mRNA in the brain and its action on the peripheral tissues.
Morita
M
M
Molecular Biological Research Laboratory, Fujisawa Pharmaceutical Co Ltd, Tsukuba, lbaraki, Japan.
Kita
Y
Y
Notsu
Y
Y
D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000187:drug effects / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*
D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D012965:Sodium Chloride