title: Time-dependent effects of EDTA on dentin structures.
abstract: The purpose of this study was to evaluate the effects of EDTA on smear layer removal and on the structure of dentin, after 1 and 10 min of application. Six extracted single-rooted teeth were instrumented to #60. Apical and coronal thirds of each root were removed, leaving a 5 mm middle third that was then cut longitudinally into two equal segments. Using 10 ml of 17% EDTA solution, halves belonging to the same root were irrigated for 1 and 10 min, respectively. All specimens were subjected to irrigation with 10 ml of 5% NaOCl. Then all the specimens were prepared for SEM evaluation. The results showed that 1 min EDTA irrigation is effective in removing the smear layer. However a 10-min application of EDTA caused excessive peritubular and intertubular dentinal erosion. Therefore we suggest that this procedure should not be prolonged >1 min during endodontic treatment.
authors.affiliation: Department of Endodontics, Hacettepe University, Faculty of Dentistry, Sihhiye, Ankara, Turkey.
authors.forename: Semra
authors.initials: S
authors.lastname: Calt
authors.forename: Ahmet
authors.initials: A
authors.lastname: Serper
mesh_terms: D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology; D002614:Chelating Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000187:drug effects* / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D018915:Root Canal Preparation; D016369:Smear Layer; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; D002614:Chelating Agents; D012388:Root Canal Irrigants; D004492:Edetic Acid; D012973:Sodium Hypochlorite