Expression of lux genes in a clinical isolate of Streptococcus pneumoniae: using bioluminescence to monitor gemifloxacin activity.
A clinical isolate of Streptococcus pneumoniae was transformed with a plasmid containing the lux operon of Photorhabdus luminescens that had been modified to function in gram-positive bacteria. Cells containing this plasmid produced light stably and constitutively, without compromising the growth rate. Light output was correlated with measurements of optical density and viable counts during exponential growth and provided a sensitive, real-time measure of the pharmacodynamics of the fluoroquinolone gemifloxacin.
Beard
S J
SJ
Faculty of Applied Sciences, University of the West of England, United Kingdom.
Salisbury
V
V
Lewis
R J
RJ
Sharpe
J A
JA
MacGowan
A P
AP
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D015870:Gene Expression / Q000187:drug effects; D005798:Genes, Bacterial / Q000502:physiology*; D006801:Humans; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D009287:Naphthyridines / Q000494:pharmacology*; D009876:Operon / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000378:metabolism
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D009287:Naphthyridines; D000077735:Gemifloxacin