PRINS, the other in situ DNA labeling method useful in cellular biology.
PRINS has proven to be an attractive alternative to FISH for in situ DNA labeling. PRINS is specific, simple, and rapid. We review some applications of PRINS involving primers specific for telomeric, human Alu, and centromeric alpha-satellite sequences. Bicolor labeling, PRINS-FISH, or PRINS-immunofluorescent combinations have been developed to enable investigations in numerous applications.
Coullin
Philippe
P
UMR1599 Génomique cellulaire des cancers, Villejuif, France. pcoullin@igr.fr
Roy
Lydia
L
Pellestor
Franck
F
Candelier
Jean-Jacques
JJ
Bed-Hom
Bertrand
B
Guillier-Gencik
Zuzana
Z
Bernheim
Alain
A
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002869:Chromosome Aberrations; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D020401:Primed In Situ Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
D017931:DNA Primers