title: [Generation of cytotoxic T lymphocytes induced with HSP-PC70 derived from tumor cells].
abstract: OBJECTIVE
  To investigate the killing effect of cytotoxic T lymphocytes (CTLs) induced with heat shock protein-peptide complexes (HSP-PC70) derived from tumor cells.
  
  
  METHODS
  Cell culture, flow cytometric analysis, affinity chromatography for protein extraction and purification, SDS-PAGE, Western blotting, CTL induction and expansion, and LDH release were used.
  
  
  RESULTS
  63.5% of the HcaF cells expressed HSP-PC70 on their membrane by FCM analysis under physiological condition, and the expression was 75.5% of the stressed cells (P < 0.01). HcaF cells had high HSP70 expression and inducibility. The cytotoxic activity of the CTLs induced with HSP70 obtained by affinity chromatography from HcaF cells was 84.2%, 47.9% and 14.8% respectively at E/T ratio of 50 : 1, 25 : 1 and 12.5 : 1. The CTLs were CD8 positive T cells. All mice which received CTL injection got long period survival (over 90 days, P < 0.01), compared with control group (10.6 days).
  
  
  CONCLUSIONS
  HSP-PC70 derived from tumor cells has strong immunogeneicity, and can activate CTLs with high peculiarity and killing activity. HSP-PC70 induced CTL adoptive immunotherapy can be a promising treatment for human to fight cancers.
authors.affiliation: Second General Surgery Department, the First Clinical College of China Medical University, Shenyang 110001, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Fu
authors.forename: R
authors.initials: R
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Yao
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D018840:HSP70 Heat-Shock Proteins