Transcription factors NF-kappaB and AP-1/c-fos in cell response to nocodazole.
The effects of nocodazole (an agent causing degradation of microtubules in vitro) on the content of transcription factors NF-kappaB and AP-1/c-fos in the nuclei of cultured cells CHO-K1 and HeLa, as well as on the activity of these factors in the nuclei of T-cell hybridoma 2B4 were studied. Immunoblotting, immunofluorescence, and gel retardation techniques revealed that in all cell lines studied nocodazole induced rapid and considerable activation (an increased content in the nuclei) of transcription factor AP-1/c-fos. In 2B4 cells, nocodazole had no effect on the activity of NF-kappaB, but in CHO-K1 and HeLa cells it caused a transient decrease, sometimes followed by an increase in the content of subunit p50. Besides, nocodazole inhibited proteolytic degradation of NF-kappaB in CHO-K1 and HeLa cells and caused a short-term decrease in the content of subunit p65 in CHO-K1 cells. The results suggest that nocodazole may interfere with the genome expression, and these alterations should be taken into consideration, whatever experimental studies this substance is used in.
Ivanova
T V
TV
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
Ivanov
V N
VN
Nadezhdina
E S
ES
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D016328:NF-kappa B / Q000187:drug effects* / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000187:drug effects* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000187:drug effects* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016328:NF-kappa B; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; D015739:Nocodazole