[Alteration of Fas/FasL gene expression in oral squamous cell carcinoma cell line Tca83 by retinoid acid].
OBJECTIVE
To study the induction of apoptosis by retinoid acid(RA) in Tca83 cell line and the expression of correlative Fas/FasL gene.


METHODS
Apoptosis of Tca83 was detected by TUNEL, the expression of Fas/FasL-mRNA was detected by RT-PCR and cell immunohistochemical technique.


RESULTS
The cell apoptosis number of Tca83 in RA group was significantly higher than that in non-RA groupe on day 5(P < 0.05). The expression of Fas-mRNA in RA group was also much higher than that in non-RA group on day 5(P < 0.05), but the level of FasLmRNR had no change. Immunohistochemistry also showed, after RA treatment, the Fas stain was enhanced.


CONCLUSIONS
The result suggests that apoptosis induced by RA may be resulted from the enhancement of Fas gene transcription and translation.
Guan
W
W
Department of Stomatology, Affiliated Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Yu
S
S
Gao
Y
Y
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D053222:Fas Ligand Protein; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008562:Membrane Glycoproteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000235:genetics*
D000970:Antineoplastic Agents; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D019014:fas Receptor; D014212:Tretinoin