[Expression and clinopathological significance of HCCA1 in hepatocellular carcinoma].
OBJECTIVE
To explore the correlation of a newly cloned HCCA1 gene expression with hepatocellular carcinoma (HCC).


METHODS
HCCA1 mRNA and protein expression were detected by Northern blotting and by immunohistochemistry respectively. Its clinopathological significance was also analyzed.


RESULTS
HCCA1 mRNA expression was found in 59 (90.8%) out of 65 patients. Of these, 54 (91.5%) showed significantly elevated expression of HCCA1 mRNA in HCC tissues. There were 9 (13.8%) patients who had low level of HCCA1 mRNA expression in paracarcinoma, and 56 (86.2%) cases showed no detectable signals. HCCA1 mRNA expression was closely related to lower tumor grading and portal vein invasion. It was noted that HCCA1 mRNA levels were higher in tumor grading III-IV group than in grading I-II group, higher in portal vein thrombus group than in non-thrombus group. Immunohistochemical staining confirmed that the HCCA1 protein was preferentially expressed in HCC tissues. Amongst 38 patients examined, 20 (52.6%) revealed positive staining with anti-HCCA1.


CONCLUSIONS
HCCA1 is a novel HCC associated gene which may play a role in HCC invasion and metastasis.
Zeng
J
J
International Cooperation Laboratory on Signal Transduction, Eastern Hepatobilliary Surgical Institute, Second Military Medical University, Shanghai 200438, China.
Wang
H
H
Wan
X
X
D000328:Adult; D000368:Aged; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018797:Cell Cycle Proteins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C456659:YY1AP1 protein, human