Analysis of the tissue marker AgNOR in leukoplaquia and oral squamous cell carcinoma.
OBJECTIVE
To determine the prognostic importance of AgNORs and ki-67 in premalignant and malignant lesions in the oral cavity, we carried out a study using retroprospective phases in which 98 histologic sections.


METHODS
26 of mild epithelial dysplasia DEL, 15 of moderate epithelial dysplasia DEM, and 57 of invasive oral squamous cell carcinoma CECO, were stained with silver colloid stain to obtain the average number and type of AgNORs, and immunologic marker Ki-67 to obtain the percentage of cells Ki-67 (+).


RESULTS
In both phases the average number of AgNORs and Ki-67 (+) cells was significantly greater in the CECO cases, compared to the dysplasias. The AgNORs type 1 were predominant in the DEL cases and the type 2 in the CECO cases. No statistically significant correlation (Sperman's simple range correlation) was found between the average numbers of AgNORS and the percentage of Ki-67 (+) cells in the three lesions studied. (DEL: p= 0.5113, DEM: p= 0.92 y CECO: p= 0.2584).


CONCLUSIONS
The results suggest that the AgNOR tissue marker can be used as a routine complementary histopathologic study, since the variations in its number and distribtion indicate existence of cell alterations in a given lesion and the use of this technique is easy and inexpensive.
Cano Montoya
Luis Carlos
LC
Departamento de Patologia del Hospital Universitario San Vicente de Paul. Universidad de Antioquia. Medellin. Colombia.
Alvarez Gómez
Gloria Jeanethe
GJ
Valencia Londoño
Walter Augusto
WA
Ramírez España
Jorge Adolfo
JA
Prada Navas
Carlos Alberto
CA
D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009697:Nucleolus Organizer Region; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D012189:Retrospective Studies
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen