Replication-dependent recruitment of the beta-subunit of DNA polymerase III from cytosolic spaces to replication forks in Escherichia coli.
The beta-subunit of DNA polymerase III is located as one or two condensed clusters within the nucleoid-occupied space in exponentially growing cells of Escherichia coli. When chromosome replication is terminated after incubation at nonpermissive temperature in a temperature-sensitive dnaC mutant, the beta-subunit is located in the cytosolic spaces of the cell poles.
Onogi
Toshinari
T
Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976, Japan. hiraga@gpo.kumamoto-u.ac.jp
Ohsumi
Katsufumi
K
Katayama
Tsutomu
T
Hiraga
Sota
S
D001425:Bacterial Outer Membrane Proteins; D002451:Cell Compartmentation; D002876:Chromosomes, Bacterial; D003600:Cytosol / Q000201:enzymology; D004258:DNA Polymerase III / Q000302:isolation & purification*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D021122:Protein Subunits; D014157:Transcription Factors / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D021122:Protein Subunits; C089746:SeqA protein, E coli; D014157:Transcription Factors; C101139:beta subunit, DNA polymerase III; D004258:DNA Polymerase III