[The inducibility of Amp C enzyme of Enterobacter cloacae and its susceptibility to enzyme inhibitor].
OBJECTIVE
To study the inducibility of Amp C enzyme of Enterobacrter cloacae and its susceptibility to enzyme inhibitor.


METHODS
The in vitro activities of the fourth generation cephalosporins-cefepime, the third generation cephalosporins-cefuroxime, cefotaxime, ceftazidime combined with beta-lactamase inhibitor sulbactam against 10 strains of ceftazidime resistant Enterobacter cloacae selected from the hospitals was studied with agar two fold dilution method. The inducibility of beta-lactam antibiotics in common use clinically to Amp C enzyme and the inhibitory action of 3 inhibitors to Amp C enzyme were studied with ultraviolet spectrophotometry and RTPCR.


RESULTS
Sulbactam can not enhance the activity of ceftazidime against Enterobacter cloacae. Cefepime has a strong activity against ceftazidime resistant Enterobacter cloacae. Imipenem has a strong inductive action to Amp C enzyme. Ro481220 has a strong inhibitory action to Amp C enzyme.


CONCLUSIONS
Infections caused by Enterobacter cloacae producing Amp C enzyme can be treated by cefepime.
Zhang
Y
Y
Institute of Clinical Pharmacology, the First Hospital, Peking University, Beijing 100083, China.
Li
J
J
Zhao
M
M
D001426:Bacterial Proteins; D002511:Cephalosporins / Q000494:pharmacology*; D016972:Enterobacter cloacae / Q000187:drug effects* / Q000201:enzymology*; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D013407:Sulbactam / Q000494:pharmacology; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000096:biosynthesis*
D001426:Bacterial Proteins; D002511:Cephalosporins; D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; D013407:Sulbactam