Morphological behavior of osteoblast-like cells on surface-modified titanium in vitro.
In recent papers, we reported the results of a study on the graded porous titanium coatings on titanium by plasma spraying and amino-group ion implantation. The paper is to preliminarily evaluate the biocompatibility of surface-modified titanium through 2, 5 and 7 days cell culture in vitro. Cell morphology was observed by a scanning electron microscope. Cell proliferation and type I collagen synthesis were measured by 3(4.5-dimethyl-thiazole-2-yl)2,5-diphenyl tetrazolium bromide (MTT) and enzyme-linked immunosorbent assay (ELISA), respectively. Our experimental results showed that osteoblast-like cells attached and spread well on surface-modified titanium. Cells were observed to grow into the pores and form extracellular matrix. MTT and ELISA results showed no detrimental effect on the development of cell. These studies support the biocompatibility of surface-modified titanium.
Yang
Yunzhi
Y
Beijing Fine Ceramics Laboratory, Tsinghua University, Beijing, China. yunzhiyang@yahoo.com
Tian
Jiemo
J
Deng
Li
L
Ong
Joo L
JL
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D002455:Cell Division; D024042:Collagen Type I / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D007477:Ions; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*
D001672:Biocompatible Materials; D024042:Collagen Type I; D004396:Coloring Agents; D007477:Ions; D013778:Tetrazolium Salts; D013844:Thiazoles; D017886:Durapatite; D014025:Titanium; C022616:thiazolyl blue