title: Sex- and strain-dependent histological features of the proximal convoluted tubular epithelium of mouse kidney: association with lysosomes containing apolipoprotein B.
abstract: In the present study, we performed comparative histological observations of ICR, BALB/c, C57BL/6, C3H/HeN and DBA/2 mice kidneys. Sex and strain differences were observed in the appearance of vacuolar structures of the proximal convoluted tubules (toluidine blue-positive granules in osmium-postfixed epoxy-resin sections). These features were especially remarkable in male DBA/2 mice. The vacuolar structures in male DBA/2 mice showed heterogeneous staining with Sudan B in frozen sections and appeared under an electron microscope as multilammelar giant dense bodies. In addition, these dense bodies showed heterogeneous acid phosphatase reactions. Immunohistochemical analyses of these structures for apolipoprotein B showed strong positive reactions. These results suggested that vacuolar structures in the proximal convoluted tubules, which were remarkable in male DBA/2 mice, were giant lysosomes containing apolipoprotein B.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Agriculture, Kagoshima University, Kagoshima-shi, Japan. yabu@vet.agri.kagoshima-u.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Yabuki
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Nishinakagawa
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism*; D004848:Epithelium / Q000033:anatomy & histology; D005260:Female; D007150:Immunohistochemistry; D007687:Kidney Tubules, Proximal / Q000033:anatomy & histology*; D008247:Lysosomes / Q000378:metabolism* / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D016612:Paraffin Embedding; D012727:Sex Characteristics; D013045:Species Specificity; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D001055:Apolipoproteins B; D000135:Acid Phosphatase