Protective effect of low dose of ascorbic acid on hepatobiliary function in hepatic ischemia/reperfusion in rats.
OBJECTIVE
The aim of this study was to investigate the effect of ascorbic acid (AA) an alterations in hepatic secretory and microsomal functions during hepatic ischemia and reperfusion.


METHODS
Rats were subjected to 60 min of hepatic ischemia, and 1 and 5 h of reperfusion. Five minutes prior to ischemia, the animals were administered either vehicle or ascorbic acid (AA) (30, 100, 300, and 1000 mg/kg) intravenously.


RESULTS
The serum aminotransferase level and lipid peroxidation were markedly higher as a result of ischemia/reperfusion. These increases were significantly attenuated by AA doses of 30 and 100 mg/kg but were augmented by dose of 1000 mg/kg. Bile flow and cholate output were markedly decreased by ischemia/reperfusion. AA doses of 30 and 100 mg/kg restored but dose of 1000 mg/kg inhibited their secretion. Both the cytochrome P(450) content and aminopyrine N-demethylase activity were decreased by ischemia/reperfusion, which were prevented by AA doses of 30 and 100 mg/kg but were aggravated by dose of 1000 mg/kg. Aniline p-hydroxylase activity was elevated by ischemia/reperfusion, and this was prevented by AA doses of 100, 300 and 1000 mg/kg.


CONCLUSIONS
Ischemia/reperfusion diminishes the hepatic secretory and microsomal functions. AA has both antioxidant and pro-oxidant effects, depending upon the dose.
Seo
Min Young
MY
College of Pharmacy, Sungkyunkwan University, 300 Chonchon-dong, Changan-gu, Suwon 440-746, South Korea.
Lee
Sun Mee
SM
D000410:Alanine Transaminase / Q000097:blood; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D001646:Bile / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism
D000975:Antioxidants; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D000410:Alanine Transaminase; D001205:Ascorbic Acid