[Effect of sensitized lymphocytes on rabbit calvarial osteoblasts].
OBJECTIVE
To study the effects of sensitized T lymphocytes on osteoblastic proliferation and differentiation.


METHODS
Sensitized lymphocytes were obtained from a rabbit sensitized by chromium, and lymphocyte cultured mediums (LCM) were collected. Osteoblasts were obtained from new-born rabbit calvaria. The osteoblasts were identified with alkaline phosphatase staining and mineralization in vitro. By osteoblast cultured in vitro, osteoblastic proliferation, and production of alkaline phosphatase and osteocalcin were evaluated in three conditions: without LCM, with LCM not stimulated by Cr(6+) and with LCM stimulated by Cr(6+).


RESULTS
The cells from rabbit calvaria were positive to ALP staining and could form calcium node in vitro. The sensitized LCM could inhibit proliferation of osteoblasts, increase alkaline phosphatase production and decrease osteocalcin production. These results all had statistically significant difference by ANOVA.


CONCLUSIONS
Sensitized T lymphocytes could decrease the number of mature osteoblasts and impede the formation of mature mineralized bone.
Lu
H
H
The department of Orthopedic, First Hospital Affiliated to Peking University, Beijing 100034, China.
Zhu
T
T
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002455:Cell Division; D002857:Chromium / Q000494:pharmacology; D010006:Osteoblasts / Q000502:physiology*; D015675:Osteocalcin / Q000096:biosynthesis; D011817:Rabbits; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D002857:Chromium; D015675:Osteocalcin; D000469:Alkaline Phosphatase