title: Mutations in sfdA and sfdB suppress multiple developmental mutations in Aspergillus nidulans.
abstract: Conidiophore morphogenesis in Aspergillus nidulans occurs in response to developmental signals that result in the activation of brlA, a well-characterized gene that encodes a transcription factor that is central to asexual development. Loss-of-function mutations in flbD and other fluffy loci have previously been shown to result in delayed development and reduced expression of brlA. flbD message is detectable during both hyphal growth and conidiation, and its gene product is similar to the Myb family of transcription factors. To further understand the regulatory pathway to brlA activation and conidiation, we isolated suppressor mutations that rescued development in strains with a flbD null allele. We describe here two new loci, designated sfdA and sfdB for suppressors of flbD, that bypass the requirement of flbD for development. sfd mutant alleles were found to restore developmental timing and brlA expression to strains with flbD deletions. In addition, sfd mutations suppress the developmental defects in strains harboring loss-of-function mutations in fluG, flbA, flbB, flbC, and flbE. All alleles of sfdA and sfdB that we have isolated are recessive to their wild-type alleles in diploids. Strains with mutant sfd alleles in otherwise developmentally wild-type backgrounds have reduced growth phenotypes and develop conidiophores in submerged cultures.
authors.affiliation: Cereon Genomics, LLC, Cambridge, Massachusetts 02139, USA. emkell@ag.arizona.edu
authors.forename: Ellen M
authors.initials: EM
authors.lastname: Kellner
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Adams
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal; D008040:Genetic Linkage; D013489:Suppression, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors
chemical_list: C063497:BRLA protein, Emericella nidulans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C092469:flbD protein, Aspergillus nidulans