[HPLC analysis of nonsteroidal antirheumatic substances in biological material. V. Fenamates].
The present paper surveys published HPLC methods estimating derivates of fenamic acid in biological materials and an HPLC method for estimation of meclofenamic acid in the samples of whole blood. Meclofenamic acid was isolated from the sample of the blood both liquid-liquid extraction and solid-liquid extraction. For determination of meclofenamic acid in the blood was preferred solid-liquid extraction. The adjust sample was analysed on reverse phase Separon SGX C-18 with the mobile phase methanol-water (pH 2.3) and detected at 220 nm. The method was used in a pharmacokinetic study of meclofenamic acid on rabbits.
Sochor
J
J
Katedra farmaceutické chemie a kontroly léciv Farmaceutické fakulty Univerzity Karlovy, Hradec Králové. sochor@faf.cuni.cz
Klimes
J
J
Sedlácek
J
J
Hnilová
M
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008469:Meclofenamic Acid / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits; D062367:ortho-Aminobenzoates / Q000097:blood* / Q000493:pharmacokinetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D062367:ortho-Aminobenzoates; D008469:Meclofenamic Acid