title: Lack of CD27-CD45RA-V gamma 9V delta 2+ T cell effectors in immunocompromised hosts and during active pulmonary tuberculosis.
abstract: In humans, the circulating pool of mycobacteria-reactive Vgamma9Vdelta2+ T cells is expanded with age and may contribute to Mycobacterium tuberculosis immunosurveillance. We observed that two subsets of Vgamma9Vdelta2+ T cells could be identified on the basis of CD27 expression in immunocompetent adults, showing that functionally differentiated gammadelta T cells have lost CD27 expression. In contrast, the CD27-CD45RA-Vgamma9Vdelta2+ T cell subset of effector cells was absent in cord blood cells from healthy newborns and lacking in the peripheral blood from HIV-infected patients. Moreover, circulating Vgamma9Vdelta2+ T cell effectors were significantly reduced in patients with acute pulmonary tuberculosis, resulting in a reduced frequency of IFN-gamma-producing cells after stimulation with nonpeptidic mycobacterial ligands. These observations indicate that monitoring and boosting gammadelta T cell effectors could be clinically relevant both in immunocompromised hosts and during active tuberculosis disease.
authors.affiliation: Laboratory of Clinical Pathology-Immunopathology, Padiglione Del Vecchio, National Institute for Infectious Diseases, Lazzaro Spallanzani, Institute for Cancer Research and Treatment, Via Portuense 292, 00149 Rome, Italy.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Gioia
authors.forename: Chiara
authors.initials: C
authors.lastname: Agrati
authors.forename: Rita
authors.initials: R
authors.lastname: Casetti
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cairo
authors.forename: Giovanna
authors.initials: G
authors.lastname: Borsellino
authors.forename: Luca
authors.initials: L
authors.lastname: Battistini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mancino
authors.forename: Delia
authors.initials: D
authors.lastname: Goletti
authors.forename: Vittorio
authors.initials: V
authors.lastname: Colizzi
authors.forename: Leopoldo P
authors.initials: LP
authors.lastname: Pucillo
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Poccia
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology; D006801:Humans; D016867:Immunocompromised Host / Q000276:immunology*; D007156:Immunologic Memory; D016130:Immunophenotyping; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000096:biosynthesis; D007399:Interphase / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens