Amplification and overexpression of c-met gene in Epstein-Barr virus-associated gastric carcinomas.
To reveal the role of oncogenes in Epstein-Barr virus (EBV)-positive gastric carcinomas, the amplification and overexpression of the c-met gene were examined by a competitive polymerase chain reaction and immunohistochemistry, respectively. The proportion of c-met amplification and overexpression in EBV-positive and -negative carcinomas did not differ significantly. The amplification and overexpression of the c-met gene in EBV-negative gastric carcinomas were significantly associated with upper location, deeper invasion and lymphatic invasion, while in EBV-positive gastric carcinomas a significant correlation with c-met activation was observed only in deeper invasion. However, none of the observed associations of c-met amplification or overexpression with clinicopathological features in the EBV-positive and -negative carcinomas differed significantly in their strength or direction. These results suggest that the amplification and overexpression of c-met gene do not play a different role in the progression and metastasis of EBV-positive and EBV-negative gastric carcinomas.
Kijima
Yuko
Y
First Department of Surgery, Center for Chronic Viral Diseases, Kagoshima University, Kagoshima, Japan. ryu23@gf6.so-net.ne.jp
Hokita
Shuichi
S
Yoshinaka
Heiji
H
Itoh
Tetsuhiko
T
Koriyama
Chihaya
C
Eizuru
Yoshita
Y
Akiba
Suminori
S
Aikou
Takashi
T
D000368:Aged; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology*
D019859:Proto-Oncogene Proteins c-met