Effect of HCV NS(3) protein on p53 protein expression in hep atocarcinogenesis.
AIM:To investigate hepatocarcinogenesis by detecting the effect of HCV NS(3) protein on p53 protein expression in hepatocellular carcinoma (HCC) and pericarcinomatous liver tissue (PCLT).METHODS:The expression of HCV NS(3) and p53 protein was detected with immunohistochemical technique (SP method) in specimens of HCC and PCLT from 47 patients with negative HBV.RESULTS:The positive rate of HCV NS(3) protein was lower in HCC (62%) than in PCLT (83%) (P < 0.025). The better differentiaton of cancer cells, the stronger expression of HCV NS(3) protein (P < 0.025). The positive rate of p53 protein in HCC (81%) was higher than in PCLT (47%) (P < 0.025). The worse differentiaton of cancer cells, the stronger expression of p53 protein (P < 0.05). The p53 protein expression was not correlated with the HCV NS(3) protein expression in HCC (P < 0.5), whereas their expression was closely related to PCLT (P < 0.01), and the expression rate of p53 protein in the cases of positive HCV NS(3) protein was higher than that in the cases of negative HCV NS(3) protein.CONCLUSION:HCV NS(3) protein may exert its hepatocarcinogenic effect in early stage on host cells by endogenous pathway which may bring about mutation of p53 gene and transformation of hepatocytes.
Feng
De-Yun
DY
Chen
Rui-Xue
RX
Peng
Yong
Y
Zheng
Hui
H
Yan
Ya-Hui
YH