Prorenins activation by an enzyme from rat plasma (PreR-Co).
The aim of the present research was to explore the capacity of PreR-Co to process prorenin purified from kidney and corpora lutea (CL) and to study its action on extrarenal tissues. The PreR-Co was obtained from plasma as a single electrophoretic band by (NH4)2SO4 precipitation, gel filtration, anti-rat albumin immunoaffinity, and ion-exchange chromatography. Prorenin free of renin was obtained after (NH4)2SO4 precipitation, gel filtration, and ion-exchange chromatography by a passage through an affinity gel of H-77 Sepharose. SDS-PAGE of supernatant and of acidic elution from gel, exhibited a single band of 43 kDa and 35 kDa, respectively; both recognized by the specific anti rat renin antibody. The isolated renin was not attacked by PreR-Co; on the contrary prorenin was completely activated. The product of PreR-Co-activated prorenin showed an analogous MW to that of renin and was recognized by the specific antibody. In addition to processing kidney prorenin, PreR-Co was able to cleave inactive renin from ovary, CL, uterus and adrenal gland homogenates. However, the amount of active renin generated from these tissues was lower than those produced by trypsin activation. PreR-Co is a good candidate for the role of the enzyme involved in tissues prorenin activation.
Vincent
Paula
P
Instituto Superior de Investigaciones Biológicas (INSIBIO)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad Nacional de Tucumán, San Miguel de Tucumán 4000, Argentina.
Adler
Conrado
C
Avila
Cesar
C
Guardia
Cecilia
C
De Vito
Eduardo
E
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D003338:Corpus Luteum / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000378:metabolism; D008970:Molecular Weight; D010053:Ovary / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism
D004792:Enzyme Precursors; D010450:Endopeptidases; D012083:Renin; C453579:PreR-Co