Dysregulated L-selectin expression on lymphocytes in patients with chronic venous insufficiency.
In this study we analysed blood samples taken from the dorsalis pedis vein and a brachial vein of 11 healthy test persons and 8 patients with venous leg ulcer under experimental venous hypertension in order to examine changes in the expression of leukocyte adhesion molecules (LFA-1 (CD11a), Mac-1 (CD11b), p150,95 (CD11c), CD18, VLA-4 (CD49d) and L-selectin (CD62L)) which are involved in the adhesion steps of leukocytes to endothelial cells for transmigration into tissues. Under orthostatic stress, lymphocytes in controls collected at the foot level showed a significant reduction in the expression of L-selectin (p=0.002), compared to those of patients. This finding suggests that venous stasis negatively influences the expression of L-selectin on leukocytes only in healthy volunteers. Patients with chronic venous insufficiency seem to suffer from a specific defect in the regulation of L-selectin shedding under orthostatic stress.
Jünger
M
M
University Hospital for Dermatology, Fleischmannstr. 42-44, D-17487 Greifswald, Germany. juenger@uni-greifswald.de
Friedrich
B
B
Hahn
J
J
Klyscz
T
T
Müller
C A
CA
Schmid-Schönbein
G W
GW
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002908:Chronic Disease; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin / Q000096:biosynthesis*; D007871:Leg Ulcer; D008214:Lymphocytes / Q000378:metabolism*; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood; D014689:Venous Insufficiency / Q000097:blood*
D015815:Cell Adhesion Molecules; D019041:L-Selectin