Cancer chemopreventive activity of flavanones on Epstein-Barr virus activation and two-stage mouse skin carcinogenesis.
To search for possible cancer chemopreventive agents from natural sources, we performed primary screening of ten flavanones isolated from plants belonging to Rutaceae and Leguminosae by examining their possible inhibitory effects on Epstein-Barr virus (EBV) early antigen activation induced by 12-O-tetradecanoylphorbol-13-acetate in Raji cells. All of the flavanones tested in this study showed inhibitory activity against EBV, without showing any cytotoxicity. Amorilin (3), which has three prenyl (3-methyl-2-butenyl) side-chains in the molecule, showed the most potent activity. Furthermore, lupinifolin (5) exhibited a marked inhibitory effect on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test. These results indicate that some of these prenylated flavanones might be valuable as potential cancer chemopreventive agents (anti-tumor promoters).
Itoigawa
Masataka
M
Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan. itoigawa@tokaigakuen-u.ac.jp
Ito
Chihiro
C
Ju-ichi
Motoharu
M
Nobukuni
Toshihiro
T
Ichiishi
Eiichiro
E
Tokuda
Harukuni
H
Nishino
Hoyoku
H
Furukawa
Hiroshi
H
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens; D004305:Dose-Response Relationship, Drug; D005260:Female; D044950:Flavanones; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008956:Models, Chemical; D012878:Skin Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D013755:Tetradecanoylphorbol Acetate; D013997:Time Factors
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D044950:Flavanones; D005419:Flavonoids; C448460:amorilin; C448459:lupinifolin; D013755:Tetradecanoylphorbol Acetate; C028610:flavanone