title: Cryopreservation of apple dormant buds and shoot tips.
abstract: Shoot tips excised from apple (Malus domestica Borkh. cv. Golden Delicious) dormant buds immediately before freezing were successfully cryopreserved using three different methods (vitrification, encapsulation-dehydration and two-step freezing). Two-step freezing produced the highest in vitro growth recovery (83%) of cryopreserved shoot tips, followed by vitrification (60%) and encapsulation-dehydration (33%). By contrast, when dormant buds were cryopreserved using the two-step freezing technique, shoot tips excised from buds after rewarming and cultured in vitro displayed only 16% recovery.
authors.affiliation: Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Hebei 066600, Changli Town, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: F
authors.initials: F
authors.lastname: Engelmann
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: D
authors.initials: D
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D027845:Malus / Q000254:growth & development*; D018520:Plant Shoots / Q000187:drug effects; D020127:Recovery of Function
chemical_list: D003451:Cryoprotective Agents