Staphylococcus aureus requires increased level of Ca(2+) or Mn(2+) to grow normally in a high-NaCl/low-Mg(2+) medium.
Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1.0 M NaCl. Cells grown in such a high-NaCl/low-Mg2+ medium exhibited the morphologic abnormality of larger than normal cells. Both sufficient growth and normal cell morphology were restored by increasing Mg2+ concentration in a high-NaCl medium, or by supplementation with either CaCl2 or MnSO4 in a high-NaCl/low-Mg2+ medium. Supplementing with BaCl2, SrCl2 or FeSO4, however, had no effect. These results indicate that Ca2+ and Mn2+ might play some essential role in the growth of Staphylococcus aureus in a high-NaCl/low-Mg2+ environment.
Ochiai
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Faculty of Pharmaceutical Science, Hokuriku University, Kanazawa, Ishikawa, Japan.
D001464:Barium / Q000378:metabolism / Q000494:pharmacology; D001622:Betaine / Q000378:metabolism; D002118:Calcium / Q000008:administration & dosage / Q000494:pharmacology*; D002122:Calcium Chloride / Q000378:metabolism; D003470:Culture Media; D005296:Ferrous Compounds / Q000378:metabolism; D008274:Magnesium / Q000008:administration & dosage / Q000494:pharmacology*; D008345:Manganese / Q000008:administration & dosage / Q000494:pharmacology*; D011074:Polyanetholesulfonate / Q000378:metabolism; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology
D003470:Culture Media; D005296:Ferrous Compounds; D001464:Barium; D001622:Betaine; D008345:Manganese; D012965:Sodium Chloride; D011074:Polyanetholesulfonate; D008274:Magnesium; D015032:Zinc; D002122:Calcium Chloride; D002118:Calcium