Involvement of GTP-binding protein in pancreatic cAMP-mediated exocytosis.
We studied cAMP-mediated exocytosis in rat pancreatic acinar cells. We monitored changes in the membrane capacitance (DeltaC), which reflects the granule fusion/retrieval process, with whole-cell patch-clamp capacitance measurement. The rise in cellular cAMP, caused indirectly by receptor activation by vasoactive intestinal polypeptide or directly by dibutyryl cyclic AMP, was able to induce an increase in DeltaC independently of cellular Ca2+. Using the latter stimulation, we estimated the magnitude of the response to internal GTPgammaS [guanosine 5'-(gamma-thio)trisphosphate] and/or GDPbetaS [guanosine 5'-(beta-thio)diphosphate]. The internal GTPgammaS and GDPbetaS amplified and depressed the response, respectively. Thus, the cellular cAMP alone can trigger granule insertion independently of cellular Ca2+ and it can be controlled by cellular GTP-binding proteins, presumably those belonging to the Rab family.
Sato
Kozo
K
Department of Physiology I, Tohoku University Graduate School of Medicine, Seiryo-cho 2-1, Aobaku, Sendai, 980-8575 Japan.
Ohsaga
Atsushi
A
Oshiro
Takako
T
Ito
Sadayoshi
S
Maruyama
Yoshio
Y
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D025802:Electric Capacitance; D005089:Exocytosis / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D010179:Pancreas / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D014660:Vasoactive Intestinal Peptide; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D003994:Bucladesine; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000109:Acetylcholine