title: Interleukin-3 and interferon beta cooperate to induce differentiation of monocytes into dendritic cells with potent helper T-cell stimulatory properties.
abstract: It was observed that interferon beta (IFN-beta) prevents the down-regulation of the interleukin-3 receptor alpha chain (IL-3Ralpha), which spontaneously occurs during culture of human monocytes. The functionality of IL-3R was demonstrated by the fact that IL-3 rescued IFN-beta-treated monocytes from apoptosis. Monocytes cultured in the presence of IFN-beta and IL-3 acquire a dendritic morphology and express high levels of HLA antigen class I and class II and costimulatory molecules. When stimulated by either lipopolysaccharide or fibroblasts expressing CD40 ligand (CD40L) transfectants, dendritic cells (DCs) generated in IFN-beta and IL-3 secreted high levels of IL-6, IL-8, and tumor necrosis factor-alpha but low levels of IL-12 in comparison with DCs generated in IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). In mixed leukocyte culture, IL-3-IFN-beta DCs induced a vigorous proliferative response of allogeneic cord blood T cells and elicited the production of high levels of IFN-gamma and IL-5 by naive adult CD4+ T cells. Finally, IL-3-IFN-beta DCs were found to produce much higher levels of IFN-alpha than IL-4-GM-CSF DCs in response to Poly (I:C) but not to influenza virus. It was concluded that monocytes cultured in the presence of IL-3 and IFN-beta differentiate into DCs with potent helper T-cell stimulatory capacity despite their low secretion of IL-12.
authors.affiliation: Laboratory of Experimental Immunology, Université Libre de Bruxelles, route de Lennik 808, B-1070 Brussels, Belgium.
authors.forename: Christel
authors.initials: C
authors.lastname: Buelens
authors.forename: Emmanuel J
authors.initials: EJ
authors.lastname: Bartholomé
authors.forename: Zoulikha
authors.initials: Z
authors.lastname: Amraoui
authors.forename: Michael
authors.initials: M
authors.lastname: Boutriaux
authors.forename: Isabelle
authors.initials: I
authors.lastname: Salmon
authors.forename: Kris
authors.initials: K
authors.lastname: Thielemans
authors.forename: Fabienne
authors.initials: F
authors.lastname: Willems
authors.forename: Michel
authors.initials: M
authors.lastname: Goldman
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D016207:Cytokines / Q000032:analysis; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D004357:Drug Synergism; D006801:Humans; D007231:Infant, Newborn; D016899:Interferon-beta / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D016185:Receptors, Interleukin-3 / Q000187:drug effects / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D016207:Cytokines; D007377:Interleukin-3; D016185:Receptors, Interleukin-3; D016899:Interferon-beta