Siderophore-mediated iron uptake in fluorescent Pseudomonas: characterization of the pyoverdine-receptor binding site of three cross-reacting pyoverdines.
Two Pseudomonas fluorescens and one Pseudomonas aeruginosa strains, although producing structurally different pyoverdines, demonstrated highly efficient cross-reactions when tested for pyoverdine-mediated iron uptake. A ferripyoverdine receptor-deficient mutant of the P. aeruginosa strain was unable to use any of the three pyoverdines. Moreover, the three strains presented each a specific outer membrane siderophore-receptor pattern. Thus, the capacity of using heterologous pyoverdines was related not to the presence of supplementary specific ferripyoverdine receptors but to the existence within the respective pyoverdine-peptide chains of a common dipeptide motif which should act as the receptor-binding site for the three pyoverdines. Other pyoverdines sharing the same motif but at another position within the peptide chain were not efficient in iron transport, demonstrating the importance of the spatial position of the binding site.
Meyer
Jean-Marie
JM
Laboratoire de Microbiologie et Génétique, UPRES-A 7010 du CNRS, ULP, Strasbourg 67083, France. meyer@gem.u-strasbg.fr
Geoffroy
Valérie A
VA
Baysse
Christine
C
Cornelis
Pierre
P
Barelmann
Insa
I
Taraz
Kambiz
K
Budzikiewicz
Herbert
H
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001665:Binding Sites; D007501:Iron / Q000378:metabolism*; D009842:Oligopeptides; D010860:Pigments, Biological / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D007501:Iron