Stability-indicating methods for the determination of doxazosin mezylate and celecoxib.
Two stability-indicating methods were developed for the determination of doxazosin mesylate (I) and celecoxib (II) in the presence of their degradation products. The first method depends on the use of first derivative spectrophotometry (D(1)) at 256, 269 nm for (I) and (II), respectively. This method determines (I) and (II) in concentration ranges of 0.8-12 and 1-20 microg ml(-1) with mean percentage accuracies of 99.21+/-0.88 and 99.59+/-1.67% for (I) and (II), respectively. The second method depends on the quantitative densitometric evaluation of thin-layer chromatography of (I) and (II) in the presence of their degradation products without any interference. Methylisobutyl ketone-glacial acetic acid-water (20:10:10) was used as a mobile phase for (I) and cyclohexane-dichloromethane-diethyleamine (50:40:10) for (II). The chromatograms were scanned at 248 and 253 nm for (I) and (II), respectively. This method determines (I) and (II) in concentration ranges of l-4 microg per spot for both drugs with mean percentage accuracies of 100.19+/-0.95 and 99.91+/-1.95% for (I) and (II), respectively. The suggested methods were used to determine doxazosin mesylate and celecoxib in bulk powder, laboratory-prepared mixtures and pharmaceutical dosage forms (cardura tablet and celebrex capsule). The results obtained by applying the proposed methods were statistically analysed and compared with those obtained by the reported methods.
Bebawy
L I
LI
National Organization For Drug Control and Research (NODCAR), 6 Hussen Kamal el Deen, Ben-el-sariat, Dokki, Giza, Egypt. a.c.afri@egyptonline.com
Moustafa
A A
AA
Abo-Talib
N F
NF
D000317:Adrenergic alpha-Antagonists / Q000032:analysis* / Q000737:chemistry; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000737:chemistry; D000068579:Celecoxib; D003720:Densitometry / Q000379:methods; D017292:Doxazosin / Q000032:analysis* / Q000737:chemistry; D004355:Drug Stability; D011720:Pyrazoles; D013053:Spectrophotometry / Q000379:methods; D013449:Sulfonamides / Q000032:analysis* / Q000737:chemistry
D000317:Adrenergic alpha-Antagonists; D000894:Anti-Inflammatory Agents, Non-Steroidal; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib; D017292:Doxazosin