Plasmodium vivax Duffy binding protein peptides specifically bind to reticulocytes.
Plasmodium vivax Duffy Binding Protein (Pv-DBP) is essential during merozoite invasion of reticulocytes. Reticulocyte binding region identification is important for understanding Pv-DBP reticulocyte recognition. Fifty 20 mer non-overlapping peptides, spanning Pv-DBP sequences, were tested in erythrocyte and reticulocyte binding assays. Ten HARBPs, mainly located in region II (Kd 50-130 nM), were High Activity Reticulocyte Binding Peptides (HARBPs); one bound to erythrocytes. Reticulocyte trypsin-, chymotrypsin- or neuraminidase- treatment affects HARBP binding differently, suggesting that these peptides have different reticulocyte-binding-sites. Some peptides bound to a Coomasie non-stainable 40 Kda band. Some HARBPs were able to block recombinant PvRII binding (Pv-DBP region II) to Duffy positive reticulocytes.
Ocampo
Marisol
M
Fundacion Instituto de Inmunología de Colombia Carrera SO No. 26-00, Bogotá, Colombia. marisolocampo@altavista.com
Vera
Ricardo
R
Eduardo Rodriguez
Luis
L
Curtidor
Hernando
H
Urquiza
Mauricio
M
Suarez
Jorge
J
Garcia
Javier
J
Puentes
Alvaro
A
Lopez
Ramsés
R
Trujillo
Mary
M
Torres
Elizabeth
E
Patarroyo
Manuel Elkin
ME
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D001665:Binding Sites; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry*; D010966:Plasmodium vivax / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism*; D017086:beta-Thalassemia / Q000097:blood
D000953:Antigens, Protozoan; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; C066001:Duffy antigen binding protein, Plasmodium; D008024:Ligands; D010455:Peptides; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins