HPLC analysis of ecdysteroids in plant extracts using superheated deuterium oxide with multiple on-line spectroscopic analysis (UV, IR, 1H NMR, and MS).
HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
Louden
Dave
D
LGC, Runcom, Cheshire, UK.
Handley
Alan
A
Lafont
Rene
R
Taylor
Steve
S
Sinclair
Ian
I
Lenz
Eva
E
Orton
Timothy
T
Wilson
Ian D
ID
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D017666:Deuterium Oxide; D026461:Ecdysteroids / Q000032:analysis*; D004867:Equipment Design; D006358:Hot Temperature; D010936:Plant Extracts / Q000032:analysis*; D013057:Spectrum Analysis
D026461:Ecdysteroids; D010936:Plant Extracts; D017666:Deuterium Oxide