Amplification of the 16S-23S rDNA spacer region for rapid detection of Clostridium chauvoei and Clostridium septicum.
Amplification of the 16S-23S rDNA spacer region by polymerase chain reaction (PCR) was used for the rapid detection of Clostridium chauvoei and C septicum. To assess its specificity, PCR was performed with total DNA from 42 strains of clostridia and three strains of other genera. PCR products specific to C chauvoei or to C septicum were generated from homologous cultures only. Clostridium chauvoer-specific or C septicum-specific amplicons were also generated from tissues of cows experimentally infected with C chauvoei or C septicum and in DNA samples from cows clinically diagnosed as having blackleg or malignant oedema. These results suggest that a species-specific PCR may be useful for the rapid and direct detection of C chauvoei and C septicum in clinical specimens.
Sasaki
Y
Y
National Veterinary Assay Laboratory, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan. sasakiy@nval.go.jp
Yamamoto
K
K
Amimoto
K
K
Kojima
A
A
Ogikubo
Y
Y
Norimatsu
M
M
Ogata
H
H
Tamura
Y
Y
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D003013:Clostridium / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D005784:Gene Amplification; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012680:Sensitivity and Specificity; D013045:Species Specificity
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S