title: Role of ICAM-3 in the initial interaction of T lymphocytes and APCs.
abstract: Antigen-independent adhesive interactions between T lymphocytes and antigen-presenting cells (APCs) are essential for scanning for specific antigens on the APC surface and for initiating the immune response. Here we show, through time-lapse imaging of live cells, that the intercellular adhesion molecule 3 (ICAM-3, also known as CD50) is clustered specifically at the region of the T lymphocyte surface that initiates contact with APCs. We describe the role of ICAM-3 in T cell-APC conjugate formation before antigen recognition, in early intracellular signaling and in cytoskeletal rearrangement. Our data indicate that ICAM-3 is important in the initial scanning of the APC surface by T cells and, therefore, in generating the immune response.
authors.affiliation: Servicio de Inmunología, Hospital de la Princesa, C/ Diego de León 62, Universidad Autónoma de Madrid, 28006 Madrid, Spain.
authors.forename: María C
authors.initials: MC
authors.lastname: Montoya
authors.forename: David
authors.initials: D
authors.lastname: Sancho
authors.forename: Grégory
authors.initials: G
authors.lastname: Bonello
authors.forename: Yves
authors.initials: Y
authors.lastname: Collette
authors.forename: Claire
authors.initials: C
authors.lastname: Langlet
authors.forename: Hai Tao
authors.initials: HT
authors.lastname: He
authors.forename: Pedro
authors.initials: P
authors.lastname: Aparicio
authors.forename: Andrés
authors.initials: A
authors.lastname: Alcover
authors.forename: Daniel
authors.initials: D
authors.lastname: Olive
authors.forename: Francisco
authors.initials: F
authors.lastname: Sánchez-Madrid
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000648:ultrastructure; D015703:Antigens, CD; D000943:Antigens, Differentiation; D002352:Carrier Proteins / Q000302:isolation & purification; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000302:isolation & purification*; D002451:Cell Compartmentation; D003599:Cytoskeleton / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D007152:Immunologic Capping; D007365:Intercellular Junctions / Q000276:immunology* / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000302:isolation & purification; D008565:Membrane Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015703:Antigens, CD; D000943:Antigens, Differentiation; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D004268:DNA-Binding Proteins; C483616:ICAM3 protein, human; C110810:LAT protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D050778:NFATC Transcription Factors; D009687:Nuclear Proteins; C084882:P-selectin ligand protein; D010750:Phosphoproteins; D011948:Receptors, Antigen, T-Cell; D014157:Transcription Factors