Cooling decreases fos-immunoreactivity in the rat after formalin injection.
The effects of cooling on inflammatory response after injection of formalin in rats were examined by counting Fos-labeled cells in the dorsal horn of the spinal cord and evaluating swelling. A small amount of dilute formalin was injected into the right hindpaw of rats. The rats were divided into Cooling (-) and Cooling (+) groups and Fos-labeled cells in the spinal cord and paw volumes were compared between the two groups. In the Cooling (+) group, Fos-labeled cells were reduced significantly and the peak time of expression was delayed compared with the Cooling (-) group. Paw volumes were significantly smaller in the Cooling (+) group than in the Cooling (-) group. These results suggest that cooling therapy is effective by delaying the progress of inflammatory reactions and provides extra time for administration of analgesics or implementation of other clinical treatments.
Kenjo
Tomomi
T
Department of Orthopedic Surgery, Fukushima Medical University, Fukushima-shi, Japan.
Kikuchi
Shinichi
S
Konno
Shinichi
S
D000818:Animals; D017679:Cryotherapy / Q000379:methods*; D046508:Culture Techniques; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D005260:Female; D005557:Formaldehyde; D016762:Genes, fos / Q000276:immunology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology* / Q000628:therapy*; D010784:Photomicrography; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013116:Spinal Cord / Q000166:cytology
D005557:Formaldehyde