title: Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons.
abstract: The effects of the certified food dye Fast Green FCF (Food Green 3) on miniature synaptic events in whole-cell voltage clamped hippocampal interneurons were examined. Fast Green FCF reduced the frequency, but did not affect the amplitude or kinetics, of miniature synaptic events in a dose-dependent manner. At 1 mg/ml (1.2 mM), the frequency was reduced to 21% of control. The half-maximum effective concentration was estimated to be 200 microg/ml (250 microM). In contrast, the dye Phenol Red neither affected frequency, amplitude nor kinetics of synaptic events. The results suggest that Fast Green FCF acts at a presynaptic locus, thereby inhibiting the release of neurotransmitter.
authors.affiliation: University of Amsterdam, Swammerdam Institute for Life Sciences, section Neurobiology, P.O. Box 94084, NL-1090 GB, Amsterdam, The Netherlands. hooft@science.uva.nl
authors.forename: Johannes A
authors.initials: JA
authors.lastname: van Hooft
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects* / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D007395:Interneurons / Q000187:drug effects* / Q000502:physiology; D008086:Lissamine Green Dyes / Q000633:toxicity*; D008297:Male; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000187:drug effects* / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects* / Q000502:physiology
chemical_list: D008086:Lissamine Green Dyes; C007704:Fast Green FCF