HLA-B*35-restricted CD8(+)-T-cell epitope in Mycobacterium tuberculosis Rv2903c.
Few human CD8(+) T-cell epitopes in mycobacterial antigens have been described to date. Here we have identified a novel HLA-B*35-restricted CD8(+) T-cell epitope in Mycobacterium tuberculosis Rv2903c based on a reverse immunogenetics approach. Peptide-specific CD8 T cells were able to kill M. tuberculosis-infected macrophages and produce gamma interferon and tumor necrosis factor alpha.
Klein
Michèl R
MR
TB Research Programme, MRC Laboratories, Fajara, The Gambia. petiet@stanford.edu
Hammond
Abdulrahman S
AS
Smith
Steve M
SM
Jaye
Assan
A
Lukey
Pauline T
PT
McAdam
Keith P W J
KP
D000942:Antigens, Bacterial / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015797:HLA-B35 Antigen / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D000942:Antigens, Bacterial; D018984:Epitopes, T-Lymphocyte; D015797:HLA-B35 Antigen; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma