title: Increasing synthetic serum substitute (SSS) concentrations in P1 glucose/phosphate-free medium improves implantation rate: a comparative study.
abstract: OBJECTIVE
  To assess the comparative efficacy of IVF medium (MediCult, with 5.2 mM glucose) and a glucose/phosphate-free medium, P1 (Irvine Scientific), and to investigate the influence of increasing the serum supplementation (synthetic serum substitute; SSS; Irvine Scientific) to P1 on embryo development and implantation.
  
  
  METHODS
  Patients were randomly assigned to IVF medium (Group 1, cycles n = 172) or P1 supplemented with 10% SSS (Group 2, cycles n = 229) according to the medium scheduled for use on the day of oocyte retrieval. Another 555 IVF consequent cycles (Group 3) were performed using increased SSS concentrations (20%) in P1 medium.
  
  
  CONCLUSIONS
  In this large series of IVF cycles, we herein demonstrate that significantly higher pregnancy and implantation rates were found when embryos were cultured in glucose/phosphate-free medium P1 supplemented with 20% SSS compared to supplementation with the lower SSS concentration and with IVF medium.
authors.affiliation: The IVF Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, Israel. dalitb@tasmc.health.gov.il
authors.forename: D
authors.initials: D
authors.lastname: Ben-Yosef
authors.forename: I
authors.initials: I
authors.lastname: Yovel
authors.forename: T
authors.initials: T
authors.lastname: Schwartz
authors.forename: F
authors.initials: F
authors.lastname: Azem
authors.forename: J B
authors.initials: JB
authors.lastname: Lessing
authors.forename: A
authors.initials: A
authors.lastname: Amit
mesh_terms: D003470:Culture Media; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate
chemical_list: D003470:Culture Media