title: HSP12 is essential for biofilm formation by a Sardinian wine strain of S. cerevisiae.
abstract: Sardinian sherry strains of S. cerevisiae form a biofilm on the surface of wine at the end of the ethanolic fermentation, when grape sugar is depleted and when further growth becomes dependent on access to oxygen. A point mutation in HSP12 or deletion of the entire gene results in inability to form this film. HSP12 encodes a heat-shock protein previously foundby others to be active during stationary phase, in cells depleted for glucose, and in cells metabolizing ethanol and fatty acids, all conditions associated with sherry biofilms.
authors.affiliation: Di.S.A.A.B.A., Sez. Microbiologia-Università di Sassari, Viale Italia 39, 07100 Sassari, Italy.
authors.forename: Severino
authors.initials: S
authors.lastname: Zara
authors.forename: G
authors.initials: G
authors.lastname: Antonio Farris
authors.forename: Marilena
authors.initials: M
authors.lastname: Budroni
authors.forename: Alan T
authors.initials: AT
authors.lastname: Bakalinsky
mesh_terms: D000483:Alleles; D001483:Base Sequence; D018441:Biofilms / Q000254:growth & development*; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D014920:Wine
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins