title: Transport of proanthocyanidin dimer, trimer, and polymer across monolayers of human intestinal epithelial Caco-2 cells.
abstract: The gut absorption of proanthocyanidins (PAs) and of the related (+)-catechin monomer was investigated with colonic carcinoma (Caco-2) cells of a human origin, grown in monolayers on permeable filters. Permeability of various radiolabeled PAs differing in their molecular weight was compared with that of the radiolabeled (+)-catechin. No toxicity was observed at PA concentrations up to the physiological concentration of 1 mM. (+)-Catechin and PA dimer and trimer had similar permeability coefficients (P(app) = 0.9-2.0 x 10(-6) cm s(-1)) close to that of mannitol, a marker of paracellular transport. Paracellular transport was also indicated by the increase of absorption after reduction of the transepithelial electric resistance through calcium ion removal. In contrast, permeability of a PA polymer with an average polymerization degree of 6 (molecular weight 1,740) was approximately 10 times lower (P(app) = 0.10 +/- 0.04 x 10(-6) cm s(-1)). PAs, particularly the most astringent PA polymer, were also adsorbed on the epithelial cells. These results suggest that PA dimers and trimers could be absorbed in vivo and that polymer bioavailability is limited to the gut lumen.
authors.affiliation: Laboratoire de Chimie Biologique, INRA, Institut National Agronomique Paris-Grignon (INA-PG), Thiverval-Grignon, France.
authors.forename: S
authors.initials: S
authors.lastname: Deprez
authors.forename: I
authors.initials: I
authors.lastname: Mila
authors.forename: J F
authors.initials: JF
authors.lastname: Huneau
authors.forename: D
authors.initials: D
authors.lastname: Tome
authors.forename: A
authors.initials: A
authors.lastname: Scalbert
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001692:Biological Transport; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000378:metabolism*; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D019281:Dimerization; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D044945:Proanthocyanidins; D013997:Time Factors
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D044945:Proanthocyanidins; C013221:proanthocyanidin