title: Dysregulated L-selectin expression on lymphocytes in patients with chronic venous insufficiency.
abstract: In this study we analysed blood samples taken from the dorsalis pedis vein and a brachial vein of 11 healthy test persons and 8 patients with venous leg ulcer under experimental venous hypertension in order to examine changes in the expression of leukocyte adhesion molecules (LFA-1 (CD11a), Mac-1 (CD11b), p150,95 (CD11c), CD18, VLA-4 (CD49d) and L-selectin (CD62L)) which are involved in the adhesion steps of leukocytes to endothelial cells for transmigration into tissues. Under orthostatic stress, lymphocytes in controls collected at the foot level showed a significant reduction in the expression of L-selectin (p=0.002), compared to those of patients. This finding suggests that venous stasis negatively influences the expression of L-selectin on leukocytes only in healthy volunteers. Patients with chronic venous insufficiency seem to suffer from a specific defect in the regulation of L-selectin shedding under orthostatic stress.
authors.affiliation: University Hospital for Dermatology, Fleischmannstr. 42-44, D-17487 Greifswald, Germany. juenger@uni-greifswald.de
authors.forename: M
authors.initials: M
authors.lastname: Jünger
authors.forename: B
authors.initials: B
authors.lastname: Friedrich
authors.forename: J
authors.initials: J
authors.lastname: Hahn
authors.forename: T
authors.initials: T
authors.lastname: Klyscz
authors.forename: C A
authors.initials: CA
authors.lastname: Müller
authors.forename: G W
authors.initials: GW
authors.lastname: Schmid-Schönbein
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002908:Chronic Disease; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin / Q000096:biosynthesis*; D007871:Leg Ulcer; D008214:Lymphocytes / Q000378:metabolism*; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D013312:Stress, Physiological / Q000097:blood; D014689:Venous Insufficiency / Q000097:blood*
chemical_list: D015815:Cell Adhesion Molecules; D019041:L-Selectin