A simple preparation of stationary phase of alkylamide reversed-phase liquid chromatograph for the separation of basic substances.
A simple preparation process of alkylamide phase for reversed-phase HPLC (RP-HPLC) is described. The process includes aminopropyltrimethoxysilane firstly reacted with octanoyl chloride, then the intermediate was coupled onto porous silica. The resultant bonded silica has a reproducible ligand surface concentration and homogenous bonded ligand distribution on the porous silica. Characterization of prepared packing was carried out with elemental analysis, solid-state 13C NMR and Fourier transform infrared (FT-IR). Chromatographic evaluations were carried out by using a mixture of organic compounds including acidic, basic and neutral analytes under methanol/water as binary mobile phase. The results showed that the stationary phase have excellent chromatographic properties and can be efficiently used for the separation of basic compounds.
Huang
Xiaojia
X
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, People's Republic of China.
Wang
Junde
J
Liu
Xueliang
X
D000577:Amides; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared
D000577:Amides; D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations