Recovery of neuroendocrine cells after injury in the aging brain of a mollusc.
Recovery after crush of neuroendocrine caudodorsal cells (CDCs) in the aging brain of the mollusc Lymnaea stagnalis, was determined as a measure of neuronal plasticity. Neuronal plasticity was determined in differently aged animals containing intact (young: 170 days) or morphologically and physiologically degraded (middle-aged: 305 days and old: 443 days) CDCs. Branching patterns and electrical and chemical connectivity and afterdischarge activity of CDCs were studied. Immediately after crush, electrical coupling and chemical transmission were absent. In all age groups partial recovery occurred within about 20 days. CDCs in old animals exhibited restricted recovery of electrical coupling and enhanced recovery of chemical transmission. In young and middle-aged animals normal afterdischarges occurred from day 8 on. In old animals abnormal afterdischarges occurred starting at day 0, becoming normal by day 12 after crush. Recovery of CDC branching was partial in all age groups. It is concluded that in the aging brain recovery of CDCs after injury does occur but is differentially restricted. Our results suggest that senescent degraded neurons still possess a considerable degree of plasticity.
Klaassen
L J
LJ
Department of Developmental Neurobiology, Faculty of Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands.
Janse
C
C
van der Roest
M
M
D000375:Aging / Q000473:pathology*; D000818:Animals; D001930:Brain Injuries / Q000473:pathology*; D004594:Electrophysiology; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008195:Lymnaea / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D009409:Nerve Crush; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000473:pathology*; D009490:Neurosecretory Systems / Q000473:pathology*
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow