[Study on the effect of COX-2's selective inhibitor on human colorectal adenoma cells proliferation].
OBJECTIVE
To investigate the mechanism of COX-2's selective inhibitor, NS-398, on human colorectal adenoma development.


METHODS
The human colorectal adenoma cells were isolated and cultured identified. The cell growth rates were assessed, when treated with NS-398, by using MTT method. Expression of COX-2 protein in human colorectal tumor was examined by immunohistochemistry.


RESULTS
23 of 30 colorectal adenomas were primary cultured successfully. NS-398 inhibited the proliferation of the cells in a dose- and time-dependent manner. COX-2 was overexpressed in colorectal adenoma(83.1%) and cancer(80.0%)compared with normal colon mucosa.


CONCLUSIONS
The key of the several culture conditions for the survival of human colorectal adenoma cells have been improved. Such as, best of the digestive time and number of cell. The cells can be inhibited by NS-398 with dose-dependent. Overexpression of COX-2 protein occurs early during colorectal carcinogenesis which contribute to tumour formation.
Qiu
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C
Department of Internal Medicine. First Hospital, West China University of Medical Sciences, Chengdu 610041, China.
Ouyang
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Q
D000236:Adenoma / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009578:Nitrobenzenes / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016861:Cyclooxygenase Inhibitors; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D051546:Cyclooxygenase 2