title: Ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) in a primary effusion lymphoma cell line treated with tetradecanoyl phorbol acetate (TPA).
abstract: The ultrastructure of Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus-8 (HHV-8) has not yet been fully elucidated, although some findings have been reported using primary effusion lymphoma (PEL) cell lines, KS-1, harboring no Epstein-Barr virus (EBV) coinfection. In the present study, detailed fine structural examination of KSHV/HHV-8 was performed after stimulation of the PEL-derived cell line KS-1 with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in vitro. While unstimulated KS-1 cells contained a small number of intranuclear virus particles associated with no extracellular mature particles, KS-1 cells stimulated with TPA produced many extracellular mature particles as well as intranuclear particles, in addition to interesting tubulo-reticular structures and aggregated tubular structures in vesicles. The induced intranuclear particles were empty, doughnut shaped, and dense cored, with outer and inner diameters of 100-110 nm and 60-70 nm, respectively. Dense-cored extracellular mature particles were 150-160 nm in diameter, and some contained doughnut-shaped cores, together with a few megaloviruses, 260 nm in outer diameter. These findings indicate that KS-1 cells treated with TPA can produce extracellular mature particles as well as intranuclear particles, which were proven to be KSHV/HHV-8.
authors.affiliation: Department of Pathology, Kochi Medical School, Kohasu, Nankoku 783-8505, Japan. ohtsukiy@kochi-ms.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Jun
authors.initials: J
authors.lastname: Iwata
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Furihata
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sonobe
authors.forename: Isao
authors.initials: I
authors.lastname: Miyoshi
authors.forename: Y.
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: J.
authors.initials: J
authors.lastname: Iwata
authors.forename: M.
authors.initials: M
authors.lastname: Furihata
authors.forename: T.
authors.initials: T
authors.lastname: Takeuchi
authors.forename: H.
authors.initials: H
authors.lastname: Sonobe
authors.forename: I.
authors.initials: I
authors.lastname: Miyoshi