title: Partial isolation and identification of hepatic stimulator substance mRNA extracted from human fetal liver.
abstract: AIM:To partially isolate and identify hepatic stimulator substance mRNA from human fetal liver tissues.METHODS:The poly (A)mRNA was extracted from human fetal liver tissues of 4-5 month gestation, fractionated by size on sucrose gradient centrifugation, translated into protein from each fraction in vitro and then its products were tested for HSS activity.RESULTS:Twenty-two 500 total RNA was obtained from human fetal liver tissues and pooled.mRNA of 420 was yielded,processed by oligo(dT)-cellulose column chromatography, then was size-fractionated by ultracentrifution on a continuous sucrose density gradient (5%-25%), and separated into 18 fractions.Translated products of mRNA in fraction 8 and 9 could produce a two-fold increase in the incorporation of( 3)H-TdR into DNA of SMMC-7721 hepatoma cells and in a heat resistant and organ-specific way.CONCLUSION:The partially purified HSS mRNA was obtained and this would facilitate the cloning of HSS using expression vectors.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.forename: Gui-Chun
authors.initials: GC
authors.lastname: Xing
authors.forename: Zu-Ze
authors.initials: ZZ
authors.lastname: Wu
authors.forename: Fu-Chu
authors.initials: FC
authors.lastname: He