Determination of a minimal deletion interval on chromosome band 8p21 in sporadic prostate cancer.
Loss of the short arm of chromosome 8 is a common event in prostatic neoplasms. Previous studies indicate that there may be up to three separate tumor suppressor genes on chromosome arm 8p, based on patterns of allelic loss. The responsible gene or genes have yet to be identified. In the present study, we used laser-capture microdissection of primary human prostate tumors and 17 microsatellite markers across chromosome band 8p21 to determine a minimal deletion interval. From an initial set of 120 cases, three tumors contained overlapping interstitial deletions on chromosome band 8p21. The three cases define an internally consistent minimal candidate tumor suppressor gene interval of approximately two megabases.
Swalwell
Jennifer I
JI
Pathogenetics Unit, Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892, USA.
Vocke
Cathy D
CD
Yang
Youfeng
Y
Walker
Jonathan R
JR
Grouse
Lynette
L
Myers
Stuart H
SH
Gillespie
John W
JW
Bostwick
David G
DG
Duray
Paul H
PH
Linehan
W Marston
WM
Emmert-Buck
Michael R
MR
D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology