Hemopoiesis-stimulating action of adamantylamide dipeptide: kinetics of increase of GM-CFC in femur and co-stimulating activity of serum, role of bone marrow stromal cells.
Chief part of hemopoietic stromal cells in mediating hemopoiesis-stimulating effects of adamantylamide dipeptide (AdDP), a synthetic immunomodulatory compound, has been determined in a series of combined in vivo/in vitro studies. Indirect stimulatory effect of AdDP on proliferation of hemopoietic progenitor cells for granulocytes and macrophages (GM-CFC) was proved to be mediated by the cells of hemopoietic microenvironment growing as adherent stromal cell populations in vitro. These results supplement previously reported findings of a positive role which is played by AdDP at modulating the interplay among stimulatory cytokines and their cellular sources, and are in consent with the idea to introduce AdDP as a constituent of the hemopoiesis- and immunity-stimulating supportive medical care.
Vacek
A
A
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno.
Hofer
M
M
Weiterová
L
L
Hoferová
Z
Z
Pipalová
I
I
Masek
K
K
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000547:Amantadine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000276:immunology*; D002453:Cell Cycle; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D004151:Dipeptides / Q000494:pharmacology*; D005260:Female; D005269:Femur / Q000166:cytology; D006098:Granulocytes / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects*; D066298:In Vitro Techniques; D007274:Injections, Intraperitoneal; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D023461:Myeloid Progenitor Cells / Q000187:drug effects; D017154:Stromal Cells / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D017077:Culture Media, Conditioned; D004151:Dipeptides; C044891:adamantylamide-alanyl-isoglutamine; D000547:Amantadine