Plant allocation to defensive compounds: interactions between elevated CO(2) and nitrogen in transgenic cotton plants.
Plant allocation to defensive compounds in response to growth in elevated atmospheric CO(2) in combination with two levels of nitrogen was examined. The aim was to discover if allocation patterns of transgenic plants containing genes for defensive chemicals which had not evolved in the species would respond as predicted by the Carbon Nutrient Balance (CNB) hypothesis. Cotton plants (Gossypium hirsutum L.) were sown inside 12 environmental chambers. Six of them were maintained at an elevated CO(2) level of 900 micromol mol(-1) and the other six at the current level of approximately 370 micromol mol(-1). Half the plants in each chamber were from a transgenic line producing Bacillus thuringiensis (Bt) toxin and the others were from a near isogenic line without the Bt gene. The allocation to total phenolics, condensed tannins, and gossypol and related terpenoid aldehydes was measured. All the treatments were bioassayed against a non-target insect herbivore found on cotton, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae). Plants had lower N concentrations and higher C:N ratios when grown in elevated CO(2). Carbon defensive compounds increased in elevated CO(2), low N availability or both. The increase in these compounds in elevated CO(2) and low N, adversely affected growth and survival of S. exigua. The production of the nitrogen-based toxin was affected by an interaction between CO(2) and N; elevated CO(2) decreased N allocation to Bt, but the reduction was largely alleviated by the addition of nitrogen. The CNB hypothesis accurately predicted only some of the results, and may require revision. These data indicate that for the future expected elevated CO(2) concentrations, plant allocation to defensive compounds will be affected enough to impact plant-herbivore interactions.
Coviella
Carlos E
CE
Department of Entomology, University of California at Riverside, Riverside, California 92521, USA. coviella@ucrac1.ucr.edu
Stipanovic
Robert D
RD
Trumble
John T
JT
D000222:Adaptation, Physiological; D000818:Animals; D001413:Bacillus thuringiensis / Q000472:pathogenicity; D001427:Bacterial Toxins / Q000378:metabolism; D001681:Biological Assay; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D003368:Gossypium / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D006072:Gossypol / Q000096:biosynthesis; D006790:Host-Parasite Interactions; D007306:Insecticides / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010572:Pest Control, Biological; D010636:Phenols / Q000378:metabolism; D010935:Plant Diseases / Q000469:parasitology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D030821:Plants, Genetically Modified; D011498:Protein Precursors / Q000378:metabolism; D018411:Spodoptera / Q000254:growth & development; D013634:Tannins / Q000096:biosynthesis; D013997:Time Factors
C029103:Bacillus thuringiensis protoxin; D001427:Bacterial Toxins; D007306:Insecticides; D010636:Phenols; D011498:Protein Precursors; D013634:Tannins; D002245:Carbon Dioxide; D002244:Carbon; D006072:Gossypol; D009584:Nitrogen