Bcr in vascular smooth muscle cells involvement of Ras and Raf-1 activation by Bcr.
Bcr gene was originally identified by its presence in the chimeric Bcr/Abl oncogene. In vascular smooth muscle cells, platelet-derived growth factor-BB (PDGF) stimulated Bcr kinase activity. The mutant PDGF receptor for PI3-K, but not for PLC-gamma binding sites, showed significantly reduced Bcr kinase activity. Bcr wild-type enhanced, whereas Bcr kinase negative form inhibited PDGF-stimulated ERK1/2 activity. A dominant negative Ras did not inhibit Bcr kinase activation, and overexpression of Bcr increased Ras/Raf-1 activity and DNA synthesis. These results demonstrated the importance of Bcr in PDGF-mediated events such as activation of Ras, Raf-1, and ERK1/2 and stimulation of DNA synthesis.
Abe
J I
JI
Center for Cardiovascular Research, University of Rochester, New York 14642, USA. jun-ichi_abe@urmc.rochester.edu
Che
W
W
Yoshizumi
M
M
Huang
Q
Q
Glassman
M
M
Ohta
S
S
Wu
Y
Y
Arlinghaus
R
R
Berk
B C
BC
D000818:Animals; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D015513:Oncogene Proteins / Q000502:physiology*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics*; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology; D018631:ras Proteins / Q000235:genetics*
D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D051562:Proto-Oncogene Proteins c-bcr; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins