Purification and characterization of an ethylene-induced antifungal protein from leaves of guilder rose (Hydrangea macrophylla).
An ethylene-induced, chitin-binding protein (designated as HM30) from leaves of Hydrangea macrophylla was identified and purified to apparent homogeneity by chitin affinity chromatography followed by FPLC on a Superose 12 column. The molecular mass of HM30 was 30,010.0 Da determined by mass spectrometry and its isoelectric point of 8.4 was estimated by isoelectric focusing. The amino acid composition of HM30 was also determined. The initial 15 amino acid residues of the N-terminal were found to be N-S-M-E-R-V-E-E-L-R-K-K-L-Q-D by automatic Edman degradation. This chitin-binding protein showed antifungal activity toward several crop fungal pathogens. Knowledge of properties of HM30 should be useful for its potential application as a plant fungicidal agent.
Yang
Qiang
Q
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, 320 Yue-yang Road, Shanghai 200031, China.
Gong
Zhen-zhen
ZZ
D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D029628:Hydrangeaceae / Q000737:chemistry* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment
D000935:Antifungal Agents; D005030:Ethylenes; D010940:Plant Proteins; D002686:Chitin; C036216:ethylene; D002688:Chitinases