Leishmania major promastigotes inhibit dendritic cell motility in vitro.
Using an in vitro transwell migration assay, we have demonstrated that products secreted by Leishmania major promastigotes inhibit the motility of dendritic cells (DC) by up to 93%. Inhibition was dose dependent and reversible. By inhibiting DC migration in vivo, L. major may therefore subvert DC from their potentially protective role during leishmaniasis.
Jebbari
Heather
H
Antigen Presentation Research Group, Department of Immunology, Imperial College School of Medicine, Northwick Park Hospital, Harrow, London HA1 3UJ, United Kingdom. hjebbari@yahoo.co.uk
Stagg
Andrew J
AJ
Davidson
Robert N
RN
Knight
Stella C
SC
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D003713:Dendritic Cells / Q000469:parasitology* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D018320:Leishmania major / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
D017077:Culture Media, Conditioned