title: Reduction in matrix-related signal suppression effects in electrospray ionization mass spectrometry using on-line two-dimensional liquid chromatography.
abstract: The effect of liquid chromatographic separation on matrix-related signal suppression in electrospray ionization mass spectrometry (LC-ESI-MS) was investigated. A method incorporating on-line two-dimensional liquid chromatography mass spectrometry (LC/LC-MS) was developed to compensate for matrix effects and signal suppression in qualitative and quantitative analysis. The LC/LC-MS(MS) approach was successfully applied for single-component and multicomponent analysis in a variety of complex matrixes. It was demonstrated that matrix-related signal suppression could be induced solely by (i) column overload, (ii) matrix component-analyte coelution, or a combination of each. Application of on-line orthogonal LC/LC separations can be effective in reducing both causes of matrix-related signal suppression effects i.e., column overload and matrix-analyte coelution for a variety of LCn/MSn applications.
authors.affiliation: Drexel University, Philadelphia, Pennsylvania 19104, USA.
authors.forename: R
authors.initials: R
authors.lastname: Pascoe
authors.forename: J P
authors.initials: JP
authors.lastname: Foley
authors.forename: A I
authors.initials: AI
authors.lastname: Gusev
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D051379:Mice; D009754:Nuts / Q000737:chemistry; D009862:Online Systems; D010575:Pesticides / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014908:Triticum / Q000737:chemistry
chemical_list: D010575:Pesticides