The effect of acclimation temperature on the fusion kinetics of lipid vesicles derived from endoplasmic reticulum membranes of rainbow trout (Oncorhynchus mykiss) liver.
Membrane fusion is an obligatory step in many vital cellular processes. The well-established enrichment of bilayer-destabilizing lipids in membranes of poikilotherms subjected to growth at low temperatures leads to the prediction that such membranes will possess a greater propensity to undergo fusion. This hypothesis was explicitly tested in the present study by determining the kinetics of fusion between small unilamellar vesicles (SUVs) prepared from endoplasmic reticulum (ER) membranes of thermally-acclimated (to 5 and 20 degrees C) rainbow trout (Oncorhynchus mykiss) liver and bovine brain phosphatidylserine (BBPS). At temperatures above 10 degrees C, ER vesicles from 5 degrees C-acclimated trout, fused more rapidly and to a greater extent with BBPS vesicles (by average factors of 1.25- and 1.45-fold, respectively) than ER vesicles of 20 degrees C-acclimated trout. At temperatures >35 degrees C, apparent fusion rates declined while the extent of fusion increased in both acclimation groups. Fusion kinetics were found to be well correlated with and limited by the physical properties and phase state of the BBPS vesicles. These results indicate that dynamic attributes of biological membranes, such as the propensity to undergo fusion, are of potential regulatory significance and are partially conserved when growth or environmental temperature changes.
Miranda
Estuardo J
EJ
Department of Biology, Arizona State University, Tempe, AZ 85287-1501, USA.
Hazel
Jeffrey R
JR
D000064:Acclimatization; D000818:Animals; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*; D005454:Fluorescence Polarization; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000201:enzymology; D008561:Membrane Fusion; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D008081:Liposomes; D010714:Phosphatidylethanolamines