Testing of skeletal implant surfaces with human fetal osteoblasts.
The effect of standard orthopaedic implant materials on osteoblast proliferation and differentiation was investigated using a human osteoblast cell culture system. Human fetal osteoblasts 1.19 were cultured on stainless steel, cobalt-chrome-molybdenum, and commercially pure titanium for 12 days. Tissue culture polystyrene was used as a control. Cell proliferation was measured by electronic cell counting and by a colorimetric proliferation assay. To assess the degree of differentiation, levels of alkaline phosphatase activity, collagen Type I, and osteocalcin production were measured. Osteocalcin gene expression was measured by reverse transcriptase-polymerase chain reaction. Electronic cell counting and proliferation assays showed lower cell numbers and delayed proliferation on stainless steel and cobalt-chrome-molybdenum compared with titanium and polystyrene. Alkaline phosphatase and osteocalcin were measured higher on titanium than on stainless steel or cobalt-chrome-molybdenum. Differences in collagen Type I production were not found. Reverse transcriptase-polymerase chain reaction showed the highest osteocalcin gene expression on titanium. The human fetal osteoblast cell line 1.19 provides a rapidly proliferating and differentiating system for testing biomaterials in which differences in osteoblast proliferation and differentiation on orthopaedic implant materials could be revealed, suggesting that the chemistry of biomaterials has a dynamic effect on proliferation and differentiation of human osteoblasts.
Hendrich
Christian
C
Department of Orthopaedic Surgery, Julius Maximilians University, Würzburg, Germany.
Nöth
Ulrich
U
Stahl
Ulrich
U
Merklein
Frank
F
Rader
Christoph P
CP
Schütze
Norbert
N
Thull
Roger
R
Tuan
Rocky S
RS
Eulert
Jochen
J
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000704:Analysis of Variance; D001672:Biocompatible Materials; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011336:Probability; D019736:Prostheses and Implants; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D001672:Biocompatible Materials; D015675:Osteocalcin; D000469:Alkaline Phosphatase