title: Membrane glycoproteins of Newcastle disease virus: nucleotide sequence of the hemagglutinin-neuraminidase cloned gene and structure/function relationship of predicted amino acid sequence.
abstract: The nucleotide sequence of the glycoprotein hemagglutinin-neuraminidase (HN) gene of the Newcastle disease virus (NDV) strain Clone-30 has been determined. The open reading frame of the HN gene contains 1731 nucleotides and encodes a protein of 577 amino acids. Three highly conserved patterns among all paramyxovirus HN glycoproteins, and one additional conserved species-specific region are present. The protein contains five potential N-glycosylation sites, all but one located in the C-terminal external domain. The secondary structure prediction shows that the C-terminal external domain is mostly arranged in beta-sheets, while alpha-helices are predominantly located in the N-terminal domain. The nucleotide sequence data of the HN gene reported in this paper has been deposited in the GenBank database, under accession number AF098289.
authors.affiliation: Departamento de Bioquímica y Biología Molecular Universidad de Salamanca, Plaza Doctores de la Reina s/n, Edificio Departamental, lab 109. E-37007, Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Sagrera
authors.forename: C
authors.initials: C
authors.lastname: Cobaleda
authors.forename: J M
authors.initials: JM
authors.lastname: González De Buitrago
authors.forename: A
authors.initials: A
authors.lastname: García-Sastre
authors.forename: E
authors.initials: E
authors.lastname: Villar
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006031:Glycosylation; D015707:HN Protein / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000378:metabolism*; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
chemical_list: D015707:HN Protein; D008562:Membrane Glycoproteins