Determination of biogenic amines in wine by multidimensional liquid chromatography with online derivatisation.
An online multidimensional liquid chromatographic system was developed for online clean-up, derivatisation and separation of biogenic amines in wines. The system consists of a cation-exchange precolumn, a derivatisation coil and an analytical column, and a column-switching valve. The entire system can be easily automated. The method proved to be quantitative and sensitive. Limits of detection were below 0.05 mg l(-1) for all amines and linearity was preserved over the tested range (0.05-15 mg l(-1)). The method was applied to the analysis of red wines of different origin.
Hyötyläinen
T
T
Department of Chemistry, University of Helsinki, Finland.
Savola
N
N
Lehtonen
P
P
Riekkola
M L
ML
D001679:Biogenic Amines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods; D014920:Wine / Q000032:analysis*
D001679:Biogenic Amines