Homocysteine induces expressions of adhesive molecules on leukocytes in whole blood.
OBJECTIVE
To observe whether homocysteine can directly alter the expressions of CD11b, CD18, CD14 and L-selectin on neutrophils, monocytes, and lymphocytes in whole blood from healthy human subjects.


METHODS
Leukocyte surface adhesive molecule expressions were analyzed by immunofluorescence flow cytometry.


RESULTS
Homocysteine at the lowest concentration (20 mumol/L) significantly increased surface adhesive molecule expressions of CD11b and CD18 on each cell type and CD14 on monocytes and neutrophils in whole blood. These effects were increased at homocysteine concentrations of 200 and 400 mumol/L, but at concentrations > or = 1 mmol/L, CD11b/CD18 and CD14 expressions on all types of leukocytes were decreased. L-selectin expression was slightly decreased on all cell types in whole blood by homocysteine.


CONCLUSIONS
Homocysteine alters leukocyte expressions of CD11b/CD18, CD14 and L-selectin on leukocytes, which may be involved into homocysteine-induced leukocyte adhesion and migration.
Guo
X
X
Center of Cardiovascular Disease, Gansu Province People's Hospital, Lanzhou 730000, China. xueweiguo@163.net
Dudman
N P
NP
D000328:Adult; D018821:CD18 Antigens / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D019041:L-Selectin / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000097:blood / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D018950:Lipopolysaccharide Receptors; D016177:Macrophage-1 Antigen; D006710:Homocysteine; D019041:L-Selectin