title: The role of DSC1 components cdc10+, rep1+ and rep2+ in MCB gene transcription at the mitotic G1-S boundary in fission yeast.
abstract: In this paper, we describe the transcription profile of a group of genes at the G1-S boundary of fission yeast in synchronously dividing mitotic cells, under a variety of different conditions. This transcription profile is unaffected in cells where either cdc10+ or cdc10-C4 are constitutively overexpressed. In contrast, overexpression of either rep1+ or rep2+ results in constitutive expression of MCB-regulated genes, suggesting that these polypeptides have important regulatory properties in controlling MCB transcription. Finally, we examine the pattern of MCB-regulated transcription in cells where the G1 period is extended. Surprisingly, we find that the wee1-50 mutation causes MCB transcription throughout the cell cycle, whereas cells re-fed after nitrogen starvation have normal expression patterns. The implications of these observations for understanding MCB-regulated transcription are discussed.
authors.affiliation: Division of Biochemistry and Molecular Biology, Institute of Biological and Life Sciences, University of Glasgow, UK.
authors.forename: S
authors.initials: S
authors.lastname: White
authors.forename: F
authors.initials: F
authors.lastname: Khaliq
authors.forename: S
authors.initials: S
authors.lastname: Sotiriou
authors.forename: C J
authors.initials: CJ
authors.lastname: McInerny
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D016193:G1 Phase; D015966:Gene Expression Regulation, Fungal; D008562:Membrane Glycoproteins / Q000737:chemistry*; D016196:S Phase; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C489911:DSC1 protein, S pombe; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C096254:Rep2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C087051:rep1 protein, S pombe