Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis.
The enzyme CwlJ is involved in the depolymerization of cortex peptidoglycan during germination of spores of Bacillus subtilis. CwlJ with a C-terminal His tag was functional and was extracted from spores by procedures that remove spore coat proteins. However, this CwlJ was not extracted from disrupted spores by dilute buffer, high salt concentrations, Triton X-100, Ca(2+)-dipicolinic acid, dithiothreitol, or peptidoglycan digestion, disappeared during spore germination, and was not present in cotE spores in which the spore coat is aberrant. These findings indicate the following: (i) the reason decoated and cotE spores germinate poorly with dipicolinic acid is the absence of CwlJ from these spores; and (ii) CwlJ is located in the spore coat, presumably tightly associated with one or more other coat proteins.
Bagyan
Irina
I
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Setlow
Peter
P
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins; D006639:Histidine; D006867:Hydrolases / Q000032:analysis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D013171:Spores, Bacterial / Q000201:enzymology / Q000254:growth & development
D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D006639:Histidine; C433796:CwlJ protein, Bacillus subtilis; D006867:Hydrolases