Rapid alignment of collagen fibers in the dermis of undermined and not undermined skin stretched with a skin-stretching device.
A controlled, quantitative histochemical study was performed in five piglets to establish changes in undermined and not undermined stretched skin. The skin was stretched with a stretching device for 30 minutes to close a large skin defect. On each flank of the piglet, at a standard position, 9 x 9-cm wounds were created under general anesthesia. On one flank, the surrounding skin was undermined cranially and caudally over a 10-centimeter area. Sections of skin biopsies obtained during stretching were stained with picrosirius red and studied with routine light microscopy and polarized light microscopy in combination with image analysis. The length of collagen fibers was analyzed as a parameter of changes in the dermis resulting from skin stretching. This newly developed quantitative method appeared to be valid, specific, and reproducible, allowing for objective determination of changes in the length of the fibers in the plain of the sections. Changes in the orientation of collagen fibers in the dermis as a result of skin stretching were thereby determined. Epidermal thickness did not change significantly under the influence of stretching forces in both undermined and not undermined skin. However, the orientation of the collagen fibers changed significantly as a result of skin stretching. In undermined wounds, parallel alignment and elongation of the fibers in the plane of the sections was already observed after 15 minutes of stretching. The fibers became aligned in the direction of the stretching force, perpendicular to the wound margin. After 30 minutes of stretching, the mean major axes of the collagen fibers were longest in the plane of the sections (p < 0.001). This meant that elongation and parallel alignment of the collagen fibers had occurred. Stretching of not undermined skin for 15 minutes resulted in significantly stronger parallel alignment in the plane of the sections as compared with undermined skin. This was less well defined after 30 minutes of stretching in not undermined skin. It is concluded that skin stretching with a skin-stretching device for 30 minutes results in significant histomorphological changes of collagen fibers in the dermis of both undermined and not undermined skin. The fibers realign rapidly as a result of stretching forces and become aligned in the direction of the stretching force, perpendicular to the wound margin. These dynamic changes in collagen fibers explain the significantly decreased wound closing tension resulting from skin stretching and explain how skin stretches beyond its inherent extensibility.
Melis
Paris
P
Department of Surgical Research, Academic Medical Center, University of Amsterdam, The Netherlands. doc.melis@hotmail.com
Noorlander
Maril L
ML
van der Horst
Chantal M A M
CM
van Noorden
Cornelis J F
CJ
D000818:Animals; D001391:Azo Compounds; D001706:Biopsy; D003094:Collagen / Q000502:physiology / Q000648:ultrastructure*; D004396:Coloring Agents; D062109:Dermatologic Surgical Procedures; D020405:Dermis / Q000473:pathology*; D004817:Epidermis / Q000473:pathology; D005260:Female; D006651:Histocytochemistry; D008859:Microscopy, Polarization; D012867:Skin / Q000293:injuries; D013552:Swine; D015626:Tissue Expansion
D001391:Azo Compounds; D004396:Coloring Agents; C009798:C.I. direct red 80; D003094:Collagen