The loss of Drosophila APG4/AUT2 function modifies the phenotypes of cut and Notch signaling pathway mutants.
A P-element line ( P0997) of Drosophila melanogaster in which the P element disrupts the Drosophila homolog of the Saccharomyces cerevisiae gene APG4/AUT2 was identified during the course of screening for cut ( ct) modifiers. The yeast gene APG4/AUT2 encodes a cysteine endoprotease directed against Apg8/Aut7 and is necessary for autophagy. The P0997 mutation enhances the wing margin loss associated with ct mutations, and also modifies the wing and eye phenotypes of Notch (N), Serrate (Ser), Delta (Dl), Hairless (H), deltex (dx), vestigial (vg) and strawberry notch (sno) mutants. These results therefore suggest an unexpected link between autophagy and the Notch signaling pathway.
Thumm
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Institut für Biochemie, Universität Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany.
Kadowaki
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T
D000595:Amino Acid Sequence; D000818:Animals; D000071183:Autophagy-Related Proteins; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins; D017344:Genes, Insect; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D009687:Nuclear Proteins / Q000378:metabolism*; D010641:Phenotype; D051880:Receptors, Notch; D012097:Repressor Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics*; D014921:Wings, Animal / Q000254:growth & development
C113755:Atg4 protein, S cerevisiae; D000071183:Autophagy-Related Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D051880:Receptors, Notch; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D003546:Cysteine Endopeptidases