title: Drosophila melanogaster ferritin: cDNA encoding a light chain homologue, temporal and tissue specific expression of both subunit types.
abstract: Drosophila melanogaster secreted ferritin like the cytosolic ferritins of other organisms is composed of two subunits, a heavy chain homologue (HCH) and a light chain homologue (LCH). We report the cloning of a cDNA encoding the ferritin LCH of this insect. As predicted from the gene sequence, it contains no iron responsive element (IRE). Northern blot analysis reveals two mRNAs that differ in length due to the choice of polyadenylation signals. Message levels vary through the life cycle of the fly and are markedly increased by high levels of dietary iron. The gut is the main site of increased message synthesis and iron preferentially increases the amount of shorter messages. Western blotting reveals that LCH is the predominant ferritin subunit in all life stages. The amount of LCH protein corresponds well with the message levels in control animals, while in iron-fed animals LCH does not increase proportionally with the message levels. In contrast, the amount of HCH is less than that would be predicted from message levels in control animals, but corresponds well in iron-fed animals. Ferritin is abundant in gut and hemolymph of larvae and adults and in ovaries of adult flies. At pupariation, ferritin becomes more abundant in hemolymph than in other tissues.
authors.affiliation: The Department of Biochemistry, University of Arizona, Tucson, AZ 85721-0088, USA.
authors.forename: T
authors.initials: T
authors.lastname: Georgieva
authors.forename: B C
authors.initials: BC
authors.lastname: Dunkov
authors.forename: S
authors.initials: S
authors.lastname: Dimov
authors.forename: K
authors.initials: K
authors.lastname: Ralchev
authors.forename: J H
authors.initials: JH
authors.lastname: Law
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D004331:Drosophila melanogaster / Q000235:genetics*; D005293:Ferritins / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D005293:Ferritins