Co-suppression mediated virus resistance in transgenic tobacco plants harboring the 3'-untranslated region of Andean potato mottle virus.
Nicotiana tabacum plants were transformed with the 3'-untranslated region of the Andean Potato Mottle Virus (APMoV) genome RNA-2. Three strategies were used: the introduction of this region in sense and in antisense orientation and of a fragment comprising the entire 3'-untranslated region from RNA-2 and part of the CP22 coat protein sequence. The transgenic lines were inoculated with the virus and different responses were observed, ranging from susceptibility to APMoV to complete immunity to virus infection, in which neither the virion nor viral RNA was detected in the inoculated leaf and leaves that emerged after inoculation. The R1 progeny from different R0 lines also showed an array of virus resistance phenotypes, which was not correlated with the zygotic state of the transgene. Resistance was positively correlated with low levels of transgene mRNA accumulation, indicating a co-suppression-mediated mechanism towards the transgenic transcripts and APMoV viral RNA.
Vaslin
M F
MF
LGMV, Departamento de Genética, Universidade Federal do Rio de Janeiro (UFRJ), Brazil.
Vidal
M S
MS
Alves
E D
ED
Farinelli
L
L
de Oliveira
D E
DE
D020413:3' Untranslated Regions; D017343:Genes, Plant; D006720:Homozygote; D007113:Immunity, Innate; D008957:Models, Genetic; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D019076:Transgenes
D020413:3' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral