[Telomerase activity of peripheral blood mononuclear cells in patients with acquired immune dysfunction].
OBJECTIVE
To investigate telomerase activity of peripheral blood mononuclear cells (PBMC) in patients with acquired immune dysfunction.


METHODS
Telomerase activity of peripheral PBMC isolated from 4 groups including healthy subjects (17), chronic hepatitis B patients (14), diabetic patients (8) and chronic renal failure patients (8) was assayed with PCR-ELISA.


RESULTS
(1) Telomerase activity was detectable at low levels in resting PBMC of all the 4 groups. It increased obviously after in vitro phytohemagglutinin (PHA) stimulation; (2) Telomerase activity of the resting PBMC in chronic hepatitis B patients was significantly lower than that in normal subjects, and was upregulated to the same level in the both groups after PHA stimulation; (3) No effect of recombinant human interleukin-12 on telomerase activity of activated T lymphocytes was found.


CONCLUSIONS
PBMC may express telomerase activity and the decreased telomerase activity of PBMC in patients with chronic hepatitis B may partially be responsible for immune dysfunction in these patients.
Huang
Y
Y
Department of Infectious Diseases, The Affiliated Xiangya Hospital of Hunan Medical University, Changsha 410008, China.
Fan
X
X
Zhang
Y
Y
Zhou
Q
Q
Tang
F
F
Yi
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D003920:Diabetes Mellitus / Q000201:enzymology* / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008875:Middle Aged; D011994:Recombinant Proteins / Q000494:pharmacology; D019098:Telomerase / Q000097:blood*; D015854:Up-Regulation
D011994:Recombinant Proteins; D018664:Interleukin-12; D019098:Telomerase