Microtubule self-organisation depends upon gravity.
The molecular processes by which gravity is transduced into biological systems are poorly, if at all, understood. Under equilibrium conditions, chemical and biochemical structures do not depend upon gravity. It has been proposed that biological systems might show a gravity dependence by way of the bifurcation properties of certain types of non-linear chemical reactions that are far-from-equilibrium. We have found that in-vitro preparations of microtubules, an important element of the cellular cytoskeleton, show this type of behaviour. On earth, the solutions show macroscopic self-ordering, and the morphology of the structures that form depend upon the orientation of the sample with respect to gravity at a critical moment at an early stage in the development of the self-organised state. An experiment carried out in a sounding rocket, showed that as predicted by theories of this type, no self-organisation occurs when the microtubules are assembled under low gravity conditions. This is an experimental demonstration of how a very simple biochemical system, containing only two molecules, can be gravity sensitive. At a molecular level this behaviour results from an interaction of gravity with macroscopic concentration and density fluctuations that arise from the processes of microtubule contraction and elongation.
Tabony
J
J
Commissariat a l'Energie Atomique, Departement de Biologie Moleculaire et Structurale, Laboratoire de Resonanc Magnetique en Biologie Metabolique, C.E.A. Grenoble, France. jtabony@cea.fr
Pochon
N
N
Papaseit
C
C
D002498:Centrifugation; D018466:Gravity Sensing / Q000502:physiology*; D006160:Guanosine Triphosphate / Q000737:chemistry; D008870:Microtubules / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D013026:Space Flight; D014404:Tubulin / Q000737:chemistry*; D014893:Weightlessness
D014404:Tubulin; D006160:Guanosine Triphosphate