[Cloning and identifying renal cell carcinoma differentially expressed genes and their significance].
OBJECTIVE
To Clone study the differentially expressed new genes in renal cell carcinoma (RCC).


METHODS
Using a technique known as suppression subtractive hybridization to construct the library which contains the differently expressing cDNAs between RCC and normal kidney cells. Then the RCC specifically expressed genes were cloned.


RESULTS
Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contained 350 positive clones. Sequence analysis were performed for 5 clones. All the sequences were unknown previously and the cDNA insert GYLZ-RCC18 had three copies. Northern blot analysis showed that GYLZ-RCC18 cDNA expressed highly in RCC, but no signal could be detected in normal kidney. Using SMART RACE technique, the full length of novel gene of GYLZ-RCC18 was obtained.


CONCLUSIONS
The highly efficient cDNA subtractive library may have formed solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel differentially expressed genes may provide an important clue for studying the mechanism of occurrence and development of RCC.
Zhang
Q
Q
Department of Urology, The First Hospital, Peking University, Beijing 100034, China.
Zhang
Z
Z
Chen
L
L
D002292:Carcinoma, Renal Cell / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary