[Effect of sijunzi decoction on gastric emptying rate in rat model of spleen deficiency syndrome].
OBJECTIVE
To observe the effect of Sijunzi decoction (SJZ) on gastric emptying rate in rat model of Spleen Deficiency Syndrome (SDS) and to explore the mechanism of "Yiqi Jianpi" (replenishing Qi and invigorating Spleen) effect with gastroduodenal myoelectrical activity and brain-gut peptide concentration.


METHODS
After intramuscular injection of reserpine for 14 days, the rat model of SDS was established. Rats were randomly divided into 5 groups, group A treated by SJZ, group B treated by Liuwei Dihuang decoction (LWDH), group C naturally recovered, group D SDS rat model, consecutively treated for 14 days, and compared with group E, the normal control. Using radioisotopic technique to monitor 30 min gastric liquid emptying rate, and using the implanted bipolar silver electrode to observe the antral and duodenal myoelectrical slow wave, and the plasma and hypothalamus levels of motilium (MOT), cholecystokinin (CCK) and somatostatin (SS) were measured by radioimmunoassay (RIA) 14 days later respectively.


RESULTS
Compared with the normal control, gastric liquid emptying rate of rat SDS model was lowered (P < 0.01, P < 0.05); the hypothalamus and plasma levels of MOT and SS reduced, both hypothalamus and plasma had higher CCK levels (P < 0.01, P < 0.05); in comparing with the controls, the rhythm, amplitude and motility index of myogastric slow wave were lower also. After treated with SJZ, the aforesaid data were normalized with insignificant difference in comparing with the normal control (P < 0.01), but the rat SDS model treated by LWDH and in natural recovery had no significant improvement.


CONCLUSIONS
SJZ can promote gastric liquid emptying rate of rat SDS model by improving the myogastric electrical movement as well as regulating the levels of MOT, CCK and SS.
Ren
P
P
Xijing Hospital, the Fourth Military Medical University, Xi'an (710032).
Huang
X
X
Zhang
L
L
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005746:Gastric Emptying / Q000187:drug effects*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013158:Splenic Diseases / Q000503:physiopathology*; D013270:Stomach / Q000503:physiopathology*; D016711:Yang Deficiency / Q000503:physiopathology*
D004365:Drugs, Chinese Herbal