Molecular genetic and traditional methods for detection of Mycobacterium tuberculosis complex (discrepancy analysis).
In the past six and half years, 862 different clinical samples [sputum, bronchoalveolar lavage, thorax puncture, cerebrospinal fluid and skin samples] were tested by Gen-probe amplified Mycobacterium tuberculosis direct test (MTD) or ligase chain reaction (LCR) or polymerase chain reaction (PCR). 239 parallel clinical samples were cultivated, and some samples were stained with Ziehl-Neelsen staining. 1-4 samples were tested per patient. 29 (12.13%) samples were positive and 177 (74.05%) samples were negative with both cultivation and molecular genetic methods. 2 (0.83%) samples were positive only on cultivation, and 31 (12.97%) samples were positive only with the molecular diagnostic methods. The differences are undoubtedly explained by the sensitivity of the molecular diagnostic methods.
Deák
J
J
Department of Clinical Microbiology, University of Szeged, Somogyi Béla tér 1, H-6725 Szeged, Hungary.
Nagy
E
E
Veréb
I
I
Weszelowszky
E
E
Somfay
A
A
Kraszkó
P
P
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D022521:Ligase Chain Reaction / Q000706:statistics & numerical data; D008297:Male; D008967:Molecular Biology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology