Effect of supplementation of vitamin E and vitamin C on brain acetylcholinesterase activity and neurotransmitter levels in rats treated with scopolamine, an inducer of dementia.
In the present study, the effects of vitamins E and C on the levels of neurotransmitters and acetylcholinesterase activity in the brains of rats treated with scopolamine, an inducer of dementia, were examined. Fifty male Sprague-Dawley rats at the age of 5 wk were divided into five groups after 1 wk of adaptation and fed five different diets for 6 wk: a no-scopolamine group, which was a scopolamine-untreated group fed only a basal diet: a scopolamine-treated group fed a basal diet; a vitamin E-supplemented scopolamine-treated group: a vitamin C-supplemented scopolamine-treated group; and a vitamins E and C-supplemented scopolamine-treated group. Scopolamine was twice administered by intraperitoneal injection (300 mg/kg, body weight), 3 d and 20 min prior to sacrifice. Brain acetylcholinesterase activity was markedly reduced by scopolamine injection. However, the supplementation of vitamins E and C in the diet significantly increased the reduced brain acetylcholinesterase activity up to the level of the scopolamine-untreated group. Brain serotonin concentration in the vitamin C-supplemented scopolamine-treated group was significantly higher than that in the scopolamine-treated group. However, there were no significant differences in brain dopamine and norepinephrine concentrations among all groups. In conclusion, supplementation with vitamin E and/or vitamin C might be useful in maintaining brain acetylcholinesterase activity at the normal level and serotonin concentration for some extent under the condition to induce dementia by scopolamine administration.
Lee
L
L
Department of Home Economics Education, Chung-Ang University, Seoul, Korea. leelilha@cau.ac.kr
Kang
S A
SA
Lee
H O
HO
Lee
B H
BH
Jung
I K
IK
Lee
J E
JE
Hoe
Y S
YS
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D003704:Dementia / Q000139:chemically induced / Q000378:metabolism; D019587:Dietary Supplements; D004298:Dopamine / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D018727:Muscarinic Antagonists / Q000633:toxicity; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012601:Scopolamine / Q000633:toxicity; D012701:Serotonin / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000494:pharmacology
D000975:Antioxidants; D018727:Muscarinic Antagonists; D018377:Neurotransmitter Agents; D014810:Vitamin E; D012701:Serotonin; D012601:Scopolamine; D000110:Acetylcholinesterase; D001205:Ascorbic Acid; D004298:Dopamine; D009638:Norepinephrine