Expression of MHC class I molecules together with antigenic peptides on filamentous phages.
The availability of combinatorial T cell epitope libraries made using a phage display system would be useful for identifying the antigens recognized by T cells of unknown specificity. To this end, we have investigated here whether single chain-MHC class I molecules (scMHC-I) could be expressed together with antigenic peptides on filamentous phages. The results show that filamentous phages can express scMHC-I. Moreover, the expressed scMHC-I was able to bind antigenic peptide. These data support the use of combinatorial scMHC/T cell epitope libraries for screening potential T cell antigens.
Kurokawa
Manae Suzuki
MS
Rheumatology, Immunology, and Genetic Program, Institute of Medical Science, St. Marianna University School of Medicine, Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8512, Japan.
Ohoka
Seido
S
Matsui
Toshihiro
T
Sekine
Taichi
T
Yamamoto
Kazuhiko
K
Nishioka
Kusuki
K
Kato
Tomohiro
T
D000941:Antigens / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology*; D017912:Inovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000941:Antigens; D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D019151:Peptide Library; D010455:Peptides