Silver electrode as a sensor for determination of zinc in cell cultivation medium.
Use of the silver electrode as a sensor for the monitoring of zinc in cell growth medium is described. Zinc at silver electrodes provides specific voltammetric signal, which is affected by solution components. Signals of zinc ions in phosphate buffer solutions with and without cell growth medium were compared. Common DMEM cell culture medium was used for the cultivation of a cell line of v-myb-transformed chicken monoblasts and its variants expressing v-jun and c-jun in a zinc-dependent manner. Electrochemical results showed zinc concentrations in the medium coincide very well with the jun expression. With respect to the low toxicity of silver for eukaryotic cells, silver electrodes represent promising tools for the determination of zinc concentrations in vivo without the potential risk of a cell culture damage.
Kizek
René
R
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, Brno, 612 65, Czech Republic.
Trnková
Libuse
L
Sevcíková
Sabina
S
Smarda
Jan
J
Jelen
Frantisek
F
D000818:Animals; D002461:Cell Line, Transformed; D002645:Chickens; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D020570:Genes, myb; D017736:Ion-Selective Electrodes; D008668:Metallothionein / Q000235:genetics; D016759:Oncogene Protein p65(gag-jun) / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D012834:Silver; D014170:Transformation, Genetic; D015032:Zinc / Q000032:analysis*
D003470:Culture Media; D016759:Oncogene Protein p65(gag-jun); D012834:Silver; D008668:Metallothionein; D015032:Zinc