[The expression of bombesin receptor in gastric cancer].
OBJECTIVE
To observe the expression of receptor mRNA and its protein in SGC 7901 cells.


METHODS
(1) RT-PCR and Southern blot hybridization were performed for detection of gastrin releasing peptide GRP-R mRNA; (2) Covalent cross-linking study with biotinyl-bombesin and enhanced chemoluminescence (ECL) was performed to identify GRP-R at protein levels in SGC 7901 cells.


RESULTS
(1) It is shown that the molecular weight of the cDNA was consistent with that of expected. Southern blot was then used to further prove that specific GRP-R mRNA was expressed by SGC 7901. (2) GRP-R protein was shown by covalent cross-linking in SGC 7901 and the molecular weight of bombesin/GRP-R in SGC 7901 cells was about 75000.


CONCLUSIONS
By using RT-PCR, Southern blot and covalent cross-linking techniques, it was demonstrated that SGC 7901 cells can express bombesin receptor mRNA and protein. The results of this study provides a basis for further researches on bombesin intracellular signal transmission pathway and the role of bombesin and bombesin receptor antagonists in oncological research.
Sun
T
T
Department of Gaseroenterology, The General Navy Hospital, Beijing 100037.
Cheng
Y
Y
Hou
M
M
Li
X
X
Chen
Y
Y
D015139:Blotting, Southern; D045744:Cell Line, Tumor; D006801:Humans; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics; D018004:Receptors, Bombesin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D018004:Receptors, Bombesin