Effect of antioxidant therapy on collagen synthesis in corrosive esophageal burns.
To investigate the efficacy of antioxidant therapy on collagen synthesis in corrosive esophageal burns, 110 Sprague-Dawley rats were divided into five groups of 22 animals each. A standard esophageal caustic burn was produced by 1 ml of 10% sodium hydroxide solution for the rats in groups B to E; group A was instilled only with 0.9% saline after preparation of the distal esophageal segment. Group A animals (controls) were uninjured and untreated. Group B had untreated esophageal burns. Esophageal burns were treated in group C with vitamin E (10 mg/kg IM), in group D with vitamin C (10 mg/kg IP), and in group E with methylprednisolone (30 mg/kg IM) on each of 5 days. Eight rats from each group were killed 4 days after initiation of the study and the abdominal esophagus was studied for tissue malondialdehyde (MDA; micromol/g protein) levels. The other rats were killed 28 days after initiation of the study and determination of hydroxyproline (HP) (microg/g tissue) levels in esophageal tissue was performed for 8 rats in each group. Histopathologic evaluation was also performed in the other 6 rats from each group. MDA levels in esophageal tissue were significantly lower in groups C (9.24 +/- 2.62, P < 0.01) and group E (6.26 +/- 2.22, P < 0.001) than in group B (12.35 +/- 1.80). HP levels were significantly lower in groups A (0.75 +/- 0.21, P < 0.001), C (1.11 +/- 0.15, P < 0.01), and E (0.96 +/- 0.15, P < 0.001) than in group B (1.40 +/- 0.20). Histopathologically, collagen deposition in the submucosa and tunica muscularis was lower in groups C and E than in group B (P < 0.05, and 0.01, respectively). Our results demonstrate that treatment with antioxidant drugs such as vitamin E and methylprednisolone decreased tissue HP levels, and thus inhibited new collagen synthesis and stricture formation in rats with alkali-induced caustic esophageal burns.
Günel
Engin
E
Department of Pediatric Surgery, Medical School of Selçuk University, Konya, Turkey.
Cağlayan
Fatma
F
Cağlayan
Osman
O
Canbilen
Aydan
A
Tosun
Murat
M
D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002057:Burns, Chemical / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004947:Esophagus / Q000293:injuries* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008775:Methylprednisolone / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012972:Sodium Hydroxide; D018709:Statistics, Nonparametric; D014810:Vitamin E / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde; D012972:Sodium Hydroxide; D003094:Collagen; D001205:Ascorbic Acid; D008775:Methylprednisolone