title: cis-Elements involved in expression of unspliced RNA in Moloney murine leukemia virus.
abstract: Murine leukemia virus (MLV) produces the unspliced RNA and the singly spliced RNA at a proper ratio at a time. To identify cis-elements involved in the production of the unspliced RNA, we examined the level of unspliced RNA in a series of mutants derived from a prototype Moloney MLV mutant gag-encoding G3.6. Our present data indicated that nt 1560-1906 region in the CA-encoding region in gag was the negative cis-element and nt 5119-5355 region, which was immediately upstream of the splice acceptor site, was the positive cis-element for expression of the unspliced RNA. It was found that the former element made expression of the unspliced RNA dependent upon the latter. These two elements were functional as discrete elements and their activities were relatively position-independent.
authors.affiliation: Department of Respiratory Medicine, University of Tokyo, Hongo, Tokyo, Bunkyo-ku, Japan.
authors.forename: Sakuo
authors.initials: S
authors.lastname: Hoshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Odawara
authors.forename: Masamichi
authors.initials: M
authors.lastname: Oshima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Hajime
authors.initials: H
authors.lastname: Takizawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshikura
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics*; D008979:Moloney murine leukemia virus / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D022821:RNA Splice Sites; D012326:RNA Splicing; D012367:RNA, Viral / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D020218:Response Elements
chemical_list: D004279:DNA, Viral; D015683:Gene Products, gag; D022821:RNA Splice Sites; D012367:RNA, Viral