title: Short-term mild hyperglycemia enhances insulin-stimulated glucose disposal in lactating goats.
abstract: This work was designed to study the effect of a 3-day mild hyperglycemia (5.3 vs. 3.3 mM) on the regulation of glucose metabolism in lactating goats. Glucose was intravenously infused at variable rates simultaneously with a constant potassium-amino acid infusion. Diet plus substrate infusion maintained net energy but not protein supply. Milk yield did not change. Skeletal muscle glucose transporter (GLUT-4) was analyzed before and after hyperglycemia. In addition, the acute effect of medium and high insulin doses on glucose turnover was measured in vivo during euglycemic and hyperglycemic hyperinsulinemic clamps under potassium and amino acid replacement. Hyperglycemia reduced the endogenous glucose appearance but increased glucose disposal. It decreased the total membrane-associated GLUT-4 protein in skeletal muscle. In contrast, it improved the acute insulin-stimulated glucose disposal. Both the level and duration (3 days) of hyperglycemia contributed to this improvement. We conclude that short-term mild hyperglycemia has similar effects in lactating goats as those already observed in nonlactating rodents or humans.
authors.affiliation: Unité Mixte de Recherches sur la Production du Lait, Institut National de la Recherche Agronomique, 35590 Saint Gilles, France. lmosque@st-gilles.rennes.inra.fr
authors.forename: Sophie
authors.initials: S
authors.lastname: Lemosquet
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Debras
authors.forename: Michèle
authors.initials: M
authors.lastname: Balage
authors.forename: Jean François
authors.initials: JF
authors.lastname: Hocquette
authors.forename: Henri
authors.initials: H
authors.lastname: Rulquin
authors.forename: Jean
authors.initials: J
authors.lastname: Grizard
mesh_terms: D000596:Amino Acids / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D004435:Eating / Q000502:physiology; D005260:Female; D005947:Glucose / Q000494:pharmacology; D051275:Glucose Transporter Type 4; D006041:Goats; D006943:Hyperglycemia / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000097:blood / Q000494:pharmacology*; D007328:Insulin / Q000097:blood / Q000494:pharmacology*; D007774:Lactation / Q000502:physiology*; D019344:Lactic Acid / Q000097:blood; D008892:Milk / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000378:metabolism; D011188:Potassium / Q000097:blood
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D019344:Lactic Acid; D005947:Glucose; D011188:Potassium