[Preparation optimization and properties of the aldehyde microscopic slides for oligonucleotide microarray fabrication].
The process for preparing the aldehyde slides was optimized and the properties of the aldehyde microscopic slides for immobilizing oligonucleotide were explored. The result shows that the concentration of aminosliane reagent plays an important role in the fluorescent background. Aldehyde slides with 2% aminosilane and 5% aldehyde treatment for 16 min and 30 min respectively immobilize oligonucleotide efficiently and have low fluorescence background. During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides. At low concentration (less than 10 mumol/L), hybridization signal has linear relationship with probe concentration, the hybridization signal reaches saturation when probe concentration is more than 20 mumol/L.
Zou
Z L
ZL
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Wang
S Q
SQ
Wang
Z Q
ZQ
D000447:Aldehydes / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005898:Glass / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000187:drug effects* / Q000379:methods; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*
D000447:Aldehydes; D015345:Oligonucleotide Probes