Peptide differential display of serum-free conditioned medium from cancer cell lines.
Conditioned medium (CM) from cultured cells is a source for screening small peptides of therapeutic or diagnostic value in cancer research. Mass spectrometry has recently enabled the profiling of peptides present in biological samples. We report a single-step extraction method to increase a chance to discover small peptides with a starting volume of 750 microl of serum-free CM. In combination with protein chip mass spectrometry, our protocol will contribute to the discovery of target peptides.
Sato
Kae
K
Growth Factor Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
Sasaki
Kazuki
K
Tsao
Ming-Sound
MS
Yamaguchi
Ken
K
D002852:Chromatography, Ion Exchange; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000378:metabolism; D010455:Peptides / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D010455:Peptides