title: Cellulose acetate decoupler for on-column electrochemical detection in capillary electrophoresis.
abstract: A new decoupler for on-column electrochemical detection in capillary electrophoresis is presented. The decoupler is constructed by etching a series of holes through the side of the separation capillary with a CO2 laser and then coating the holes with cellulose acetate. The decoupler shows isolation of the detection circuit for separation currents up to 30 microA. Detection limits below 1 nM were achieved for four model compounds, including anions, neutrals, and cations, using the laser-etched decoupler. This decoupler design combines excellent mechanical stability, effective shunting of high separation currents, and ease of manufacture.
authors.affiliation: Department of Chemistry, The University of Kansas, Lawrence 66045, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Osboum
authors.forename: C E
authors.initials: CE
authors.lastname: Lunte
mesh_terms: D002482:Cellulose / Q000031:analogs & derivatives*; D004298:Dopamine / Q000032:analysis; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005841:Gentisates; D006873:Hydroquinones / Q000032:analysis; D062385:Hydroxybenzoates / Q000032:analysis
chemical_list: D005841:Gentisates; D006873:Hydroquinones; D062385:Hydroxybenzoates; C005062:acetylcellulose; D002482:Cellulose; C010925:2,5-dihydroxybenzoic acid; D004298:Dopamine