title: Sudden increase in Pseudomonas aeruginosa nosocomial strains with broad host range transfer of antibiotic resistance.
abstract: We investigated transfer of antibiotic resistance from 51 multiply resistant strains of Pseudomonas aeruginosa isolated from seriously ill patients in the Frankfurt University Clinics. Nine isolates directly transferred resistance to three recipient strains used. Ticarcillin and cephalothin resistance determinants were accepted from eight isolates, and in one case a kanamycin resistance determinant was transferred. The total spectrum of resistance transferred demonstrated that several donor strains transferred a different set of resistance determinants to all three recipient strains. Two P. aeruginosa isolates transferred spectrum of seven resistance determinants including ceftazidime, cefepime and aztreonam, three isolates transferred five determinants and four isolates transferred four resistance determinants. The fact that identical spectra of multiple drug resistance were transferred to recipient strains belonging to three different species (Escherichia coli, Proteus mirabilis and P. aeruginosa), indicates a broad host range in all three transferable genetic elements not observed in previous transfers from P. aeruginosa strains.
authors.affiliation: Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic. blahova@upkm.sk
authors.forename: J
authors.initials: J
authors.lastname: Blahová
authors.forename: K
authors.initials: K
authors.lastname: Králiková
authors.forename: V
authors.initials: V
authors.lastname: Krcméry
authors.forename: V
authors.initials: V
authors.lastname: Schäfer
mesh_terms: D003227:Conjugation, Genetic; D003428:Cross Infection / Q000382:microbiology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics