Measurement of free choline in plant leaves by capillary electrophoresis.
A low pH capillary electrophoresis (CE) was used for the measurement of free choline in plant leaves. Choline in the leaf extract was first converted to the benzoyl ester and put into CE. A well-resolved peak in the electropherogram was easily obtained. Involvement of enzymes in a two-step oxidation of choline to glycine betaine was evaluated in different plant species with the same method developed for glycine betaine and betaine aldehyde.
Zhang
J
J
Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo, Japan.
Okubo
A
A
Yamazaki
S
S
D002794:Choline / Q000032:analysis* / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D007887:Fabaceae / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D003368:Gossypium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry*
D002794:Choline; D005998:Glycine