p16 gene methylation in colorectal cancers associated with Duke's staging.
OBJECTIVE
To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.


METHODS
Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.


RESULTS
p16 methylation was detected in 42% of the tumors.Dukes'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%).


CONCLUSIONS
p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.
Yi
J
J
Department of Cell Biology, Shanghai Second Medical University, 280 Chongqing South Road, Shanghai 200025, China. yijing@shsmu.edu.cn
Wang
Z W
ZW
Cang
H
H
Chen
Y Y
YY
Zhao
R
R
Yu
B M
BM
Tang
X M
XM
D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16 / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction