Primary myelodysplastic syndrome with normal cytogenetics: utility of 'FISH panel testing' and M-FISH.
The myelodysplastic syndromes (MDS) are a clinically heterogeneous group of hematologic disorders. Cytogenetic analysis is crucial as it can provide both diagnostic and prognostic information. Herein, 32 cytogenetically normal patients with primary MDS were analyzed both by multiple FISH probes on interphase nuclei (FISH panel testing) and by M-FISH (metaphase nuclei). One patient had a chromosome 13q-arm deletion, while the remaining 31 patients had normal results. These findings confirm standard cytogenetics as an excellent technique in identifying the common chromosomal abnormalities associated with MDS and suggest limited utility for either a FISH panel test or M-FISH in primary MDS.
Ketterling
Rhett P
RP
Department of Laboratory Medicine and Pathology, Mayo Clinic/Foundation, 200 First Street S.W., Rochester, MN 55905, USA.
Wyatt
William A
WA
VanWier
Scott A
SA
Law
Mark
M
Hodnefield
Janice M
JM
Hanson
Curtis A
CA
Dewald
Gordon W
GW
D000754:Anemia, Refractory, with Excess of Blasts / Q000175:diagnosis / Q000235:genetics; D001854:Bone Marrow Cells / Q000473:pathology*; D002467:Cell Nucleus / Q000235:genetics; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D006402:Hematologic Diseases / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D008677:Metaphase; D009006:Monosomy; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000473:pathology; D015588:Observer Variation; D012016:Reference Values; D014314:Trisomy