Inverse regulation of interleukin-6 (IL-6) and IL-6 receptor in histamine deficient histidine decarboxylase-knock-out mice.
Interleukin-6, a multifunctional cytokine upon binding to its receptor on hepatocytes regulates production of acute phase proteins involved in local and systemic inflammation. Gene expression and biosynthesis of IL-6 and its receptor (IL-6 R/gp130) is under complex regulation. Histamine, in addition to its principal role in immediate type hypersensitivity has been described to modulate IL-6 production and expression of IL-6 receptor. In this study, the IL-6 and IL-6 receptor expression was examined in histamine deficient histidine decarboxylase (HDC) knock-out mouse model. Our data suggest that in histamine deficient mice the inducibility of IL-6 is significantly reduced, whilst more IL-6 receptor/gp130 mRNA expresses in the liver than in wild type (HDC(+/+)) mice. These in vivo findings confirm earlier in vitro results and emphasize the efficacy of antihistamines in local IL-6 related processes.
Horváth
B V
BV
Department of Genetics, Cell and Immunobiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4, 1089, Budapest, Hungary.
Falus
A
A
Tóth
S
S
Szalai
Cs
C
Lázár-Molnár
E
E
Holub
M Cs
MC
Buzás
E
E
Nagy
A
A
Fulop
A K
AK
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D050822:Cytokine Receptor gp130; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005787:Gene Frequency; D006632:Histamine / Q000235:genetics / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008099:Liver / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D019947:Receptors, Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D053606:Receptors, OSM-LIF
D015703:Antigens, CD; C494200:Il6st protein, mouse; D015850:Interleukin-6; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507841:Lifr protein, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D019947:Receptors, Interleukin-6; D053606:Receptors, OSM-LIF; D050822:Cytokine Receptor gp130; D006632:Histamine; D006640:Histidine Decarboxylase