title: Improved evidence for linkage on 6p and 5p with retrospective pooling of data from three asthma genome screens.
abstract: To improve our ability to identify genes for complex diseases, evaluation of new methods that retrospectively pool genotype and phenotype data collected by multiple centers is important. Availability of three whole-genome screens enabled us to compare two methods, pooling raw data and meta-analysis. Multipoint linkage analyses were performed on two outcomes, total serum IgE levels and asthma affection status, using an improved Haseman-Elston algorithm. Two regions showed stronger evidence for linkage using covariate-adjusted pooled data, compared with any individual sample. Both methods for pooling data identified strong linkage to Z-transformed logeIgE levels at a location between D6S1019 and D6S426, and to the asthma trait at D5S268. In conclusion, retrospective analysis of pooled genome scan data is a potentially powerful and useful method to examine both positive and negative evidence for linkage of quantitative and categorical phenotypes across populations.
authors.affiliation: Department of Epidemiology and Biostatistics, Case Western Reserve University, R203A Rammelkamp Building, 2500 MetroHealth Drive, Cleveland, OH 44109, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Iyengar
authors.forename: K B
authors.initials: KB
authors.lastname: Jacobs
authors.forename: L J
authors.initials: LJ
authors.lastname: Palmer
mesh_terms: D000328:Adult; D001249:Asthma / Q000453:epidemiology / Q000235:genetics*; D002648:Child; D002874:Chromosome Mapping / Q000706:statistics & numerical data*; D002895:Chromosomes, Human, Pair 5; D002896:Chromosomes, Human, Pair 6; D005260:Female; D005820:Genetic Testing; D005828:Genetics, Population; D005858:Germany; D006801:Humans; D008297:Male; D015201:Meta-Analysis as Topic; D008957:Models, Genetic; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D014481:United States