title: Levels of mast-cell growth factors in plasma and in suction skin blister fluid in adults with mastocytosis: correlation with dermal mast-cell numbers and mast-cell tryptase.
abstract: BACKGROUND
  Mast-cell accumulation has been observed in the skin and other organs of patients with systemic indolent mastocytosis (SM). The basis for this pathologic increase is not fully understood.
  
  
  OBJECTIVE
  We sought to determine levels of mast-cell growth factors in the skin and plasma of patients with SM, patients with atopic dermatitis (AD), and healthy individuals and to correlate these levels to dermal mast-cell numbers and levels of mast-cell tryptase.
  
  
  METHODS
  Skin suction blister fluid and plasma levels of stem-cell factor, IL-3, IL-4, IL-6, vascular endothelial growth factor, and total mast-cell tryptase were analyzed by means of ELISA. The number of mast cells was determined in a biopsy section taken from adjacent skin.
  
  
  RESULTS
  Mast-cell numbers in the dermis were higher in patients with SM compared with numbers in patients with AD (P <.001) or in healthy control subjects (P <.0001) and correlated with tryptase levels in both skin blister fluid (P <.0001) and plasma (P <.0001). Stem-cell factor and vascular endothelial growth factor levels in the skin blister fluid and plasma of patients with SM were not significantly different from those in patients with AD or healthy control subjects. IL-3 and IL-4 levels were below the limit of detection. IL-6 levels were significantly increased in the plasma of patients with SM compared with in plasma of patients with AD (P <.002) and healthy control subjects (P <.0001) and correlated with plasma tryptase levels (P <.05) and dermal mast-cell numbers (P <.02).
  
  
  CONCLUSIONS
  Because elevated levels of IL-6 could contribute to the fever, fatigue, and osteoporosis observed in patients with SM and because IL-6 is antiapoptotic for mast cells, IL-6 could potentiate the biologic consequences of this disease.
authors.affiliation: Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1881, USA.
authors.forename: Knut
authors.initials: K
authors.lastname: Brockow
authors.forename: Cem
authors.initials: C
authors.lastname: Akin
authors.forename: Mary
authors.initials: M
authors.lastname: Huber
authors.forename: Linda M
authors.initials: LM
authors.lastname: Scott
authors.forename: Lawrence B
authors.initials: LB
authors.lastname: Schwartz
authors.forename: Dean D
authors.initials: DD
authors.lastname: Metcalfe
mesh_terms: D000328:Adult; D000368:Aged; D001768:Blister / Q000378:metabolism*; D002452:Cell Count; D003876:Dermatitis, Atopic / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000032:analysis; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis; D008222:Lymphokines / Q000032:analysis; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology*; D008415:Mastocytosis / Q000378:metabolism*; D008875:Middle Aged; D012697:Serine Endopeptidases / Q000378:metabolism*; D012867:Skin / Q000473:pathology*; D019089:Stem Cell Factor / Q000032:analysis*; D053802:Tryptases; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D015850:Interleukin-6; D008222:Lymphokines; D019089:Stem Cell Factor; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D012697:Serine Endopeptidases; D053802:Tryptases