[Expression of a snake venom plasminogen activator TSV-PA in insect cells].
A snake venom gene TSV-PA was inserted into the donor plasmids pFastBacHTa and expressed in Tn-5B1-4 cells. SDS-PAGE analysis revealed that the molecular weight of expressed product of TSV-PA were 33 kD. It was also proved by Western blot. The result of enzyme activity showed that TSV-PA protein expressed in insect cells had a higher activity.
Cao
Y
Y
Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
Yu
G S
GS
Yang
S L
SL
Gong
Y
Y
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003435:Crotalid Venoms / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007313:Insecta / Q000166:cytology / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D012697:Serine Endopeptidases; D014162:Transfection
D003435:Crotalid Venoms; D006023:Glycoproteins; D012697:Serine Endopeptidases; C093329:TSV-PA protein, Trimeresurus stejnegeri