Albutensin A and complement C3a decrease food intake in mice.
Albutensin A (Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg) derived from serum albumin dose-dependently decreased food intake after intracerebroventricular (10-50 nmol/mouse) or peripheral (0.3-1.0 micromol/mouse) administration in fasted conscious ddY mice. Albutensin A delayed gastric emptying and elevated blood glucose levels. Although albutensin A showed low affinity for bombesin receptor, it decreased food intake in bombesin receptor knockout mice, indicating that its inhibitory effect on feeding was not mediated through bombesin receptor. Then, we investigated whether the albutensin A-induced decrease in food intake was mediated by complement C3a and C5a receptors, because albutensin A had affinities for these receptors. Des-Arg-albutensin A, lacking affinity for C3a and C5a receptors, did not inhibit food intake. We found for the first time that centrally administered C3a (10-100 pmol/mouse) by itself decreased food intake in fasted mice. In contrast, C5a increased food intake after central injection. Based on these results, we conclude that the inhibitory effect of albutensin A on food intake is mediated through the C3a receptor.
Ohinata
Kousaku
K
Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University, Gokasho Uji, 611-0011, Kyoto, Japan.
Inui
Akio
A
Asakawa
Akihiro
A
Wada
Keiji
K
Wada
Etsuko
E
Yoshikawa
Masaaki
M
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism; D015926:Complement C3a / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D009842:Oligopeptides / Q000494:pharmacology*; D011485:Protein Binding; D044087:Receptor, Anaphylatoxin C5a; D018004:Receptors, Bombesin / Q000235:genetics; D011951:Receptors, Complement / Q000737:chemistry*; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D015703:Antigens, CD; D001786:Blood Glucose; D009842:Oligopeptides; D044087:Receptor, Anaphylatoxin C5a; D018004:Receptors, Bombesin; D011951:Receptors, Complement; C418002:albutensin A; D015926:Complement C3a