title: Real-time antifungal susceptibility screening aids management of invasive yeast infections in immunocompromised patients.
abstract: We examined the utility of a semi-solid agar antifungal susceptibility screening (SAAS) test in real-time management of eight immunocompromised patients with invasive yeast infections. Tests of amphotericin B and fluconazole concentrations of 0.5 and 2 mg/L and 1, 8 and 40 mg/L, respectively, were performed on Candida albicans (two), Candida tropicalis (two), Candida krusei (one), Candida glabrata (one) and Trichosporon species (spp.) (two). All but the Trichosporon spp. and C. glabrata isolates were resistant to fluconazole at > or = 40 mg/L, and patients were successfully managed accordingly. Real-time antifungal susceptibility screening can assist in clinical management of invasive yeast infections.
authors.affiliation: Department of Medicine, Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Box 041, Tufts University School of Medicine, 750 Washington Street, Boston, MA 02111, USA. shadley@lifespan.org
authors.forename: Susan
authors.initials: S
authors.lastname: Hadley
authors.forename: José A
authors.initials: JA
authors.lastname: Martinez
authors.forename: Laura
authors.initials: L
authors.lastname: McDermott
authors.forename: Barbara
authors.initials: B
authors.lastname: Rapino
authors.forename: David R
authors.initials: DR
authors.lastname: Snydman
mesh_terms: D000328:Adult; D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D002176:Candida albicans / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification; D024901:Drug Resistance, Multiple, Bacterial / Q000276:immunology; D006801:Humans; D016867:Immunocompromised Host / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D008875:Middle Aged; D009181:Mycoses / Q000188:drug therapy* / Q000276:immunology; D014250:Trichosporon / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents