[The effect of lactate-buffered peritoneal dialysis fluid in human peritoneal mesothelial cells function].
OBJECTIVE
To investigate the effect of lactate-buffered peritoneal dialysis fluid (L-PDF) on human peritoneal mesothelial cells (HPMC) function.


METHODS
Mesothelial cells from human omentum were isolated and cultured in vitro. Proliferation of HPMC was detected by MTT assay. The quantity of interleukin-8 (IL-8) and fibronectin (FN) in culture supernatants was determined by ELISA. Reverse transcription and polymerase chain reaction amplification were used to detect the expression of IL-8 mRNA in HPMC. Total cellular protein in these supernatants was estimated by using Lowry method.


RESULTS
L-PDF inhibited proliferation of HPMC in a time-dependent manner. The levels of IL-8 and FN in the supernatants were remarkably high, and expression of IL-8 mRNA was up-regulated in HPMC exposure to L-PDF. During a subsequent recovery period of 48 hours in culture medium containing lipopolysaccharide (LPS, 10 mg/L), enterotoxin of staphylococcus aureus (10 mg/L) or TNFalpha(10 x 10(6) U/L), the levels of FN in the supernatants increased (P < 0.05). The levels of IL-8 also increased in HPMC stimulated with TNFalpha(P < 0.01).


CONCLUSIONS
L-PDF inhibited proliferation of HPMC and increased levels of IL-8 and FN in the HPMC.
Duan
S
S
Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410011, China.
Liu
F
F
Xiao
P
P
Xia
J
J
Peng
Y
Y
Ye
Y
Y
Luo
J
J
D015314:Dialysis Solutions / Q000494:pharmacology*; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis; D007773:Lactates / Q000494:pharmacology; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000166:cytology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D015314:Dialysis Solutions; D016209:Interleukin-8; D007773:Lactates; D014409:Tumor Necrosis Factor-alpha