title: Pregrowth-desiccation: a simple and efficient procedure for the cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells.
abstract: Rice embryogenic suspension cells were successfully cryopreserved by a pregrowth-desiccation procedure. Cells were precultured in liquid AA medium containing 0.175 mol/L sucrose for 3 d and then in liquid AA medium containing 0.4 mol/L sorbitol for 1 d. After air-drying for about 20 h to a water content of 10%, the cells were placed into cryotubes and quenched into liquid nitrogen. Using this pregrowth-desiccation procedure, a survival rate of 96+/-6% (TTC reduction assay) or 100% (cell clump regrowth) was achieved. Cryostored cells revived very quickly during the recovery culture and they retained the ability to regenerate fertile plants. In conclusion, air-drying, a method usually employed in cryopreservation of seeds or shoot tips, can be used as a simple and efficient procedure for the cryopreservation of precultured rice suspension cells.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310012, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Zhang
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.forename: H W
authors.initials: HW
authors.lastname: Bian
authors.forename: M Y
authors.initials: MY
authors.lastname: Zhu
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D003890:Desiccation; D012275:Oryza / Q000254:growth & development*; D012639:Seeds / Q000166:cytology*
chemical_list: D003470:Culture Media