Involvement of protein kinase C and tyrosin kinase in tumoricidal activation of macrophage induced by Streptococcus pneumoniae type II capsular polysaccharide.
Capsular polysaccharide type 2 (PS) from Streptococcus pnemoniae induced the secretory and cellular macrophage response. However, the exact mechanism by which PS regulates the macrophage functions remains unclear. In this study, we examined signal molecules which may participate in PS-elicited responses by macrophages. Our data demonstrated that tumoricidal activation of macrophages induced by PS was inhibited by either protein kinase C (PKC) inhibitor, H7 or protein tyrosine kinase (PTK) inhibitor, genistein. In addition, these inhibitors blocked the production of TNF-alpha and NO in PS-stimulated macrophages. Furthermore, PS-induced cell activation is possibly mediated by Toll-like receptor 2. These data suggest that PKC and PTK are involved in the activation of macrophages with PS.
Um
Sung-Hee
SH
Division of Immunopharmacology, College of Pharmacy, Sungkyunkwan University, Suwon, Kyunggi-do, South Korea.
Rhee
Dong-Kwon
DK
Pyo
Suhkneung
S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology*; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000737:chemistry*; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000970:Antineoplastic Agents; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D008562:Membrane Glycoproteins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D051195:Toll-Like Receptor 2; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C