Residence time of netilmicin in tears.
OBJECTIVE
To determine the concentration of netilmicin in tears after eye drop administration.


METHODS
A clinical study was carried out on 32 healthy volunteers. Subjects were divided into four groups and underwent tear collection at 5, 10, 20, and 60-minutes after drug administration, respectively. Tear samples were collected through capillary suction from the inferior conjunctival "cul-de-sac" and analyzed by high-pressure liquid chromatography (HPLC).


RESULTS
Netilmicin concentration in tears decreased after a first order kinetics, a best-fit curve was drawn and the minimum inhibitory concentration (MIC90) intersection for the most common ocular pathogens was calculated.


CONCLUSIONS
The extrapolation of the curve shows that the concentration of netilmicin on the ocular surface can be effective against microorganisms more than 120 minutes after eye drop instillation.
Scuderi
Anna Claudia
AC
SIFI SpA, Catania, Italy.
De Lazzari
Alessandra
A
Miano
Fausto
F
Zola
Pietro
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001682:Biological Availability; D002648:Child; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D006801:Humans; D008297:Male; D008875:Middle Aged; D009428:Netilmicin / Q000493:pharmacokinetics*; D013666:Tears / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D009428:Netilmicin