title: Quantitation of natural killer cell precursors in man.
abstract: A technique was developed to measure the frequency of natural killer cell precursors (NKpf) in human peripheral blood mononuclear cell (PBMC) samples. Functional maturity of NK cells was reflected in their ability to lyse target cells from the K562 cell line. During the development of the technique, venous blood was taken from one healthy adult and assayed at different times to avoid individual variation. The technique was based on the principle of limiting dilution analysis. The NKpf assay was set up with a range of cell dilutions from 40,000 to 625 per 100 microl/well in 96-well culture plates. At the end of the culture period, the K562 cell line labelled with europium (Eu-K562) was added and the Eu-release was measured in culture supernatants using time-resolved fluorometry. The NKpf value differed between individuals and was influenced by the length of time in culture, being maximal at day 5. Maturation of NKp required the continuous presence of recombinant interleukin 2 (rIL-2), or rIL-15, both being equally effective. In the absence of cytokines, the functional NK cells declined rapidly beyond 24 h in culture. Irradiated allogeneic cells appeared to substitute in part for cytokines, but the numbers of allo-activated NKpf were lower than those observed when allo-activated NKpf were cultured with rIL-2. This suggested selective activation by the allogeneic stimulus of subsets of NKp or rIL-2-rescue of NKp subsets destined for apoptotic cell death. Alternatively, the increased frequency could have been attributable to activation of precursors of natural killer-T cells (NK-Tp). This assay is suitable for estimating the total number of precursors of functional NK cells in the blood of patients.
authors.affiliation: Department of Transplantation Sciences, University of Bristol, Paul O'Gorman Life Centre, Southmead Hospital, Westbury-on-Trym, BS10 5NB, England, UK.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Gharehbaghian
authors.forename: K M Gausul
authors.initials: KM
authors.lastname: Haque
authors.forename: Carol
authors.initials: C
authors.lastname: Truman
authors.forename: John
authors.initials: J
authors.lastname: Newman
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Bradley
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology / Q000276:immunology