Study of an accelerated solvent extraction procedure for the determination of acaricide residues in honey by high-performance liquid chromatography-diode array detector.
An accelerated solvent extraction (ASE) procedure has been optimized for the determination of synthetic acaricides (amitraz, bromopropylate, cymiazole, coumaphos, T-fluvalinate, and flumethrin) and their residues in honey by reversed-phase high-performance liquid chromatography. The effects of experimental variables such as solvent composition, temperature, static extraction time, and solvent flush volume on the ASE efficiency have been studied. The acaricides were extracted by hexane-propanol (1/3, vol/vol) at 95 degrees C and 2.000 psi for 8 min. Recovery values of between 53 and 108% were achieved with the different substances, with coefficients of variation between 2 and 13% and limits of detection from 0.01 to 0.2 microg/g.
Korta
E
E
Departamento de Química Analítica, Facultad de Ciencias, Universidad del País Vasco, Bilbao, Spain.
Bakkali
A
A
Berrueta
L A
LA
Gallo
B
B
Vicente
F
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination; D006722:Honey / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D007306:Insecticides; D010573:Pesticide Residues; D012997:Solvents