title: [The application of Apo-1/Fas to evaluate apoptosis of myocardial cells in patients with congestive heart failure].
abstract: OBJECTIVE
  To study the changes of serum level of Apo-1/Fas in patients with congestive heart failure (CHF) and evaluate apoptosis of failing myocardial cells.
  
  
  METHODS
  Strepavidin-Biotin ELISA was used to determine serum level of Apo-1/Fas, interleukin-6 (IL-6) and tumor necrosis factor (TNF alpha) in 60 patients with CHF. Cardiac ejection fraction (EF) of the patients were measured by acusson 128XP/10 echocardiograph.
  
  
  RESULTS
  Serum levels of Apo-1/Fas and TNF alpha in class III and IV patients with CHF were significantly higher than those in class I and II (P < 0.01). Serum levels of IL-6 in all the patients were obviously higher than those in controls (P < 0.05 and 0.01) and the levels in class III and IV patients were significantly higher than those in class I and II (P < 0.05). Serum levels of Apo-1/Fas in patients with EF < 55 percent were higher than in those with EF > 55 percent (P < 0.05).
  
  
  CONCLUSIONS
  Serum level of Apo-1/Fas in patients with CHF reflects a state of apoptosis in failing myocardial cells. IL-6 and TNF alpha have important effects on immune regulation of myocardial cell apoptosis.
authors.affiliation: Department of Cardiovascular, Zhujiang Hospital, First Military Medical University, Guangzhou 510282.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: B
authors.initials: B
authors.lastname: Jiao
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: X
authors.initials: X
authors.lastname: Qian
authors.forename: B
authors.initials: B
authors.lastname: Mo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006333:Heart Failure / Q000276:immunology / Q000473:pathology*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D019014:fas Receptor / Q000097:blood*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor