[Key technologies in image analysis of comparative genomic hybridization].
Comparative genomic hybridization has become a new technique in molecular-cytogenetics, and it has found significant applications in tumor pathology. Image analysis is an important part of comparative genomic hybridization. It analyzes the fluorescent images by many steps and finally determines whether there is any abnormality in the copy number of the test tumor tissue. This paper expatiates on the key steps in the image analysis of comparative genomic hybridization, including background correction, chromosome segmentation, chromosome axis determination, karyotyping and determining ratio profile. The future trend is also discussed.
Ni
J
J
State Key Laboratory of Intelligent Technology and Systems of China, Department of Automation, Tsinghua University, Beijing 100084.
Ji
L
L
Wang
M
M
D003582:Cytogenetics / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping