[High-level production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by feb-batch culture of Alcaligenes eutrophus].
Fermentation strategies for production P (3HB-co-3HV) from glucose and propionic (or valeric) acid by Alcaligenes eutrophus were studied. During the culture, we controlled pH of the broth by feeding precusors of 3HV- propionic or valeric acid after Ammonia feeding stopped. When propionic acid were used as the precusor, for 50 hours, we obtained a cell dry weight, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content and a 3HV fraction of 149.9 g/L, 124.9 g/L, 83.3% and 12.4 mol%, respectively, with a PHA productivity of 2.50 g h-1 L-1. When valeric acid were used as the precusor, for 45 hours, we obtained a cell dry weight, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content and a 3HV fraction of 160.2 g/L, 119.0 g/L, 74.2% and 17.7 mol%, respectively, with a PHA productivity of 2.64 g h-1 L-1. Prior to this study, it hasn't been reported to obtain such high level productivity and 3HV fraction at the same time by Alcaligenes eutrophus.
Cai
Y B
YB
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100081, China.
Liu
M Q
MQ
Yi
Z H
ZH
Chen
Q
Q
Weng
W Q
WQ
D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D000421:Alcaligenes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D010421:Pentanoic Acids / Q000378:metabolism* / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism; D011422:Propionates / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
D010421:Pentanoic Acids; D011108:Polymers; D011422:Propionates; C013056:beta-hydroxyvaleric acid; C038780:n-pentanoic acid; D005947:Glucose; C029658:propionic acid; D020155:3-Hydroxybutyric Acid