[Studies on lignocellulolytic enzymes production and biomass degradation of Pleurotus sp2 and Trametes gallica in wheat straw cultures].
Pleurotus sp2 and Trametes gallica were selected in this assay because of their high activities of lignocellulolytic enzymes and the enzyme peaks appeared at the early stage of liquid state fermentation. Solid state fermentation was also investigated for their abilities and behaviors of enzyme-production. The capabilities and characteristics of the two strains in degrading biomass were studied. When Pleurotus sp2 was incubated in wheat straw powder containing the liquid medium of low-nitrogen, no-carbon and high inorganic salt, the activities of MnP and Lac reached the peaks on the tenth day, but the activities of hemicellulases reached the peak on the 40th day. Pleurotus sp2 caused 17.6% of biomass loss. When T. gallica was incubated in wheat straw powder containing the liquid medium of hlig-nitrogen, or low-nitrogen, no-carbon and high inorganic salt, the activities of MnP reached the peaks on the tenth day, the lac and hemicelluloses on the 40th day, and the lignin peroxidases reached the peaks on the 50th day, and it caused more than 64% of biomass loss. Among them the hemicellulose was degraded by 71.96%, and the cellulose 66.21%. T. gallica was very capable of degrading lignin of wheat straw and caused 34.37% loss during 20 days, 46. 71% loss during 30 days and 70.14% loss during 60 days. It was interesting that T. gallica degraded lignin preferentially with respect to cellulose, which was very beneficial to biopulping of paper industry.
Xie
J
J
Molecular Biology Laboratory, College of Life Science, Sichuan University, Chengdu 610064, China.
Sun
X
X
Ren
L
L
Zhang
Y Z
YZ
D001487:Basidiomycota / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D018533:Biomass; D002241:Carbohydrates / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D042845:Laccase; D008031:Lignin / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism; D020076:Pleurotus / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors; D014908:Triticum / Q000737:chemistry
D002241:Carbohydrates; D003470:Culture Media; D004798:Enzymes; D008345:Manganese; D003300:Copper; D002482:Cellulose; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; D005947:Glucose; D009584:Nitrogen