Study of angiogenesis induced by metastatic and non-metastatic liver cancer by corneal micropocket model in nude mice.
AIM:To study the angiogenesis induced by liver cancer with different metastatic potentials using corneal micropocket model in nude mice.METHODS: Corneal micropockets were created in nude mice. Tumor tissues and liver tissues were implanted into the corneal micropockets. Angiogenesis was observed usinga digital camera under slit-lamp biomicroscope,and compared among different grafts and incision alone. Vascular responses were recorded in regard to the range, number and length of new blood vessels toward the grafts or incisions.RESULTS:Vascular responses induced by tumor tissues were greater than those by incision alone and liver tissue grafts. LCI-D20 induced more intensive angiogenesis than LCI-D35.CONCLUSION:Highly metastatic liver cancer LCI D20 was more angiogenic than low metastatic cancer LCI D35 and liver tissue. Micropocket was a useful model to study dynamic process of angiogenesis in vivo.
Sun
Hui-Chuan
HC
Li
Xiao-Ming
XM
Xue
Qiong
Q
Chen
Jun
J
Gao
Dong-Mei
DM
Tang
Zhao-You
ZY