title: Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector.
abstract: Here we report the production of transgenic pigs that express enhanced green fluorescent protein (eGFP). Porcine oocytes were matured in vitro in a serum-free, chemically defined maturation medium, subsequently infected with a replication deficient pseudotyped retrovirus, fertilized and cultured in vitro before being transferred to a recipient female. Two litters were born from these embryo transfers; one pig from each litter was identified as transgenic and both expressed eGFP. From a tool in basic research to direct applications in production agriculture, domestic livestock capable of expressing foreign genes have many scientific applications.
authors.affiliation: Department of Animal Sciences, University of Missouri-Columbia, 65211, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Cabot
authors.forename: B
authors.initials: B
authors.lastname: Kühholzer
authors.forename: A W
authors.initials: AW
authors.lastname: Chan
authors.forename: L
authors.initials: L
authors.lastname: Lai
authors.forename: K W
authors.initials: KW
authors.lastname: Park
authors.forename: K Y
authors.initials: KY
authors.lastname: Chong
authors.forename: G
authors.initials: G
authors.lastname: Schatten
authors.forename: C N
authors.initials: CN
authors.lastname: Murphy
authors.forename: L R
authors.initials: LR
authors.lastname: Abeydeera
authors.forename: B N
authors.initials: BN
authors.lastname: Day
authors.forename: R S
authors.initials: RS
authors.lastname: Prather
mesh_terms: D000383:Agriculture; D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000032:analysis; D004624:Embryo Transfer; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis; D008297:Male; D009865:Oocytes; D012190:Retroviridae; D013552:Swine / Q000235:genetics*
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins