Characterization of N-acyl homoserine lactone overproducing mutants of Burkholderia multivorans ATCC 17616.
Burkholderia multivorans ATCC 17616 ordinarily produces insufficient amounts of N-acyl homoserine lactones (AHLs) to promote AHL-dependent formation of the pigment violacein by the reporter strain Chromobacterium violaceum CV026. We have isolated AHL-overproducing mutants of strain 17616 by screening for variants which do cross-feed AHLs to strain CV026. Nucleotide-sequence analysis of the bmuIR locus which specifies AHL synthase (BmuI) and AHL-binding transcriptional activator protein (BmuR) indicated that the increased capacity to produce AHLs was not a consequence of changes upstream or internal to the bmuI or bmuR genes. We conclude that the mutations leading to AHL overproduction lie outside the bmuI/bmuR locus.
Yao
Fude
F
Department of Microbiology, University of Massachusetts, Amherst, MA 01003 USA.
Zhou
Hongwei
H
Lessie
T G
TG
D015107:4-Butyrolactone / Q000031:analogs & derivatives*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001681:Biological Assay; D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives*; D008025:Ligases / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D012641:Selection, Genetic; D015534:Trans-Activators / Q000235:genetics
D001426:Bacterial Proteins; C449817:BmuR protein, Burkholderia multivorans; D015534:Trans-Activators; D006714:Homoserine; D008025:Ligases; C449816:N-acylhomoserine lactone synthase; D015107:4-Butyrolactone