title: Dominant-negative Rho, Rac, and Cdc42 facilitate the invasion process of Vibrio parahaemolyticus into Caco-2 cells.
abstract: To clarify the invasive process of Vibrio parahaemolyticus, an invasion assay was performed using cells expressing dominant negative small GTPases of the Rho family. This assay showed that the dominant negative host phenotype facilitates bacterial invasion, suggesting that the mechanism of V. parahaemolyticus invasion differs from that reported for other invasive bacteria.
authors.affiliation: Department of Bacterial Infections. Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan. yukihiro@biken.osaka-u.ac.jp
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
authors.forename: Toshio
authors.initials: T
authors.lastname: Kodama
authors.forename: Takashige
authors.initials: T
authors.lastname: Kashimoto
authors.forename: Vlademir
authors.initials: V
authors.lastname: Cantarelli
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Horiguchi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nagayama
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Iida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
mesh_terms: D000199:Actins / Q000378:metabolism; D018938:Caco-2 Cells; D006801:Humans; D014736:Vibrio parahaemolyticus / Q000472:pathogenicity*; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000502:physiology*; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000502:physiology*
chemical_list: D000199:Actins; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein