Preferential initiation of PC12 neurites in directions of changing substrate adhesivity.
When PC12 cells are grown on substrates showing a gradient of nonspecific adhesion, they preferentially initiate neurites in directions of changing adhesivity, whether that change is in the direction of increasing or decreasing adhesivity. This preference for changing adhesivity is ablated both by C. difficile toxin A, which inhibits all Rho-family GTPases, and by C. botulinum C3 exoenzyme, which specifically inhibits Rho.
Murnane
Aeneas C
AC
Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA.
Brown
Kimberly
K
Keith
Charles H
CH
D036002:ADP Ribose Transferases / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D001905:Botulinum Toxins; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D004768:Enterotoxins / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D016716:PC12 Cells; D051381:Rats; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors
D000199:Actins; D001427:Bacterial Toxins; D004768:Enterotoxins; C041348:tcdA protein, Clostridium difficile; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D020741:rho GTP-Binding Proteins