title: Effect of acetazolamide on outward permeability of blood-retina barrier using differential vitreous flyorophotometry.
abstract: OBJECTIVE
  To measure fluorescein (F) and fluorescein monoglucuronide (FG) concentrations in the vitreous and evaluate the effect of acetazolamide (AZM) on the outward permeability of the blood-retina barrier (BRB) using differential vitreous fluorophotometry (DVF).
  
  
  METHODS
  DVF was performed 180 minutes after intravenous injection of AZM (5 mg/kg) and 50 mg of sodium fluorescein in six rabbits (AZM group). DVF also was performed in six rabbits injected intravenously with only 50 mg of sodium fluorescein (control group). The F/FG ratio was calculated based on the concentrations of F and FG obtained by DVF. DVF also was performed 180 minutes after 50 mg of intravenous injection of sodium fluorescein in five rabbits given probenecid (150 mg/kg) intraperitoneally (probenecid group).
  
  
  RESULTS
  The average F/FG ratio was 0.36 +/- 0.17 (range, 0.22-0.66) in the AZM group, which was significantly smaller than the control value of 0.74 +/- 0.22 (range, 0.50-1.60). The average F/FG ratio at 180 minutes after injection was 1.51 +/- 0.46 (range, 0.94-2.00) in the probenecid group, which was significant higher (p < 0.05) than that of the AZM or control group.
  
  
  CONCLUSIONS
  This study showed that the F/FG ratio might be a good indicator of the estimated outward permeability of the BRB using DVF and that AZM may accelerate the outward active transport function of the BRB.
authors.affiliation: Department of Ophthalmology, Asahikawa Medical College, Asahikawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Takahashi
authors.forename: F
authors.initials: F
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Hikichi
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D001813:Blood-Retinal Barrier / Q000187:drug effects*; D002199:Capillary Permeability / Q000187:drug effects; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D019793:Fluorescein / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D015590:Fluorophotometry; D011339:Probenecid / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors; D014822:Vitreous Body / Q000378:metabolism
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D005452:Fluoresceins; C041357:fluorescein glucuronide; D000086:Acetazolamide; D011339:Probenecid; D019793:Fluorescein