title: Structure and phylogeny of the crustacean hyperglycemic hormone and its precursor from a hydrothermal vent crustacean: the crab Bythograea thermydron.
abstract: The structure of a well-known neurohormone involved in homeostasis regulation and stress response, the crustacean hyperglycemic hormone, was investigated in the deep-sea hydrothermal vent crab Bythograea thermydron. The neuropeptide was isolated from neurohemal organs (sinus glands) and its biological activity checked using an homologous bioassay. Partial amino acid sequence was established by a combination of Edman chemistry and mass spectrometry. Then, the sequence of the cDNA encoding the hormone precursor was determined. The preprohormone is composed of a 29 amino acid signal peptide, followed by a 41 amino acid associated peptide flanking the 72 amino acid hyperglycemic hormone. Comparison of these data with other known crab hyperglycemic hormone and prohormone sequences was performed using phylogenetic analysis methods.
authors.affiliation: Groupe Biogenēse des Peptides Isome'res, UMR CNRS Physiologie et Physiopathologie, UPMC, Bat A, case courrier 256, 7 Quai Saint-Bernard, 75252 cedex 05, Paris, France. Jean-Yves.Toullec@snv.jussieu.fr
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Toullec
authors.forename: Joëlle
authors.initials: J
authors.lastname: Vinh
authors.forename: Jean Pierre
authors.initials: JP
authors.lastname: Le Caer
authors.forename: Bruce
authors.initials: B
authors.lastname: Shillito
authors.forename: Daniel
authors.initials: D
authors.lastname: Soyez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins; D001181:Arthropods; D001483:Base Sequence; D001681:Biological Assay; D001786:Blood Glucose / Q000378:metabolism; D003386:Brachyura; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006458:Hemolymph / Q000378:metabolism; D007447:Invertebrate Hormones; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics*; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry*; D021382:Protein Sorting Signals; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D060829:Arthropod Proteins; D001786:Blood Glucose; D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D010455:Peptides; D011498:Protein Precursors; D021382:Protein Sorting Signals; C015947:hyperglycemic hormone, crustacean; D012313:RNA