Ca(2+) response to cADPr during maturation and fertilization of starfish oocytes.
During the reinitiation of the meiotic cycle (maturation) induced by the hormone 1-methyladenine (1-MA), starfish oocytes undergo structural and biochemical changes in preparation for successful fertilization. Previous work has shown that the sensitivity of internal Ca(2+) stores to InsP(3) increases during maturation of the oocytes. Since Astropecten auranciacus oocytes also respond to cADPr, we have studied whether the response to cADPr also changes during maturation. We have found that the photoactivation of injected cADPr in immature oocytes immediately induces multiple patches of Ca(2+) release in the cortical region. The Ca(2+) signal then spreads from these initial points of increase to the entire cell. In mature oocytes, the uncaging of cADPr induces instead a single (or at most a dual) initial point of Ca(2+) release, which is immediately followed by the formation of a cortical Ca(2+) flash and then by the globalization of the wave and by the elevation of the fertilization envelope. External Ca(2+) plays a role in the Ca(2+) responses. Inhibition of L-type Ca(2+) channels does not affect the initial Ca(2+) release, but abolishes the cortical flash and impairs the elevation of the fertilization envelope. External Ca(2+) has other effects, as shown by the irregular appearance of the surface of oocytes incubated in Ca(2+)-free sea water. The sequence of Ca(2+) responses induced by cADPr in mature oocytes mimics those seen at fertilization, i.e., a first localized Ca(2+) increase followed by a cortical flash and by the globalization of the Ca(2+) signal. As in the case of maturation, L-type Ca(2+) channel blockers abolish the sperm induced cortical flash.
Nusco
Gilda A
GA
Laboratory of Cell Biology, Stazione Zoologica "A. Dohrn" Villa Comunale, Naples, I-80121, Italy.
Lim
Dmitri
D
Sabala
Pawel
P
Santella
Luigia
L
D000246:Adenosine Diphosphate Ribose / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling; D036563:Cyclic ADP-Ribose; D005306:Fertilization; D007700:Kinetics; D008540:Meiosis; D008845:Microinjections; D009249:NADP / Q000031:analogs & derivatives* / Q000737:chemistry; D009543:Nifedipine / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology*; D010777:Photochemistry; D013215:Starfish / Q000196:embryology*
D002121:Calcium Channel Blockers; D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D009249:NADP; C024376:NAADP; D009543:Nifedipine