Formation of metabolites during biodegradation of linear alkylbenzene sulfonate in an upflow anaerobic sludge bed reactor under thermophilic conditions.
Biodegradation of linear alkylbenzene sulfonate (LAS) was shown in an upflow anaerobic sludge blanket reactor under thermophilic conditions. The reactor was inoculated with granular biomass and fed with a synthetic medium and 3 micromol/L of a mixture of LAS with alkylchain length of 10 to 13 carbon atoms. The reactor was operated with a hydraulic retention time of 12 h with effluent recirculation in an effluent to influent ratio of 5 to 1. A sterile reactor operated in parallel revealed that sorption to sludge particles initially accounted for a major LAS removal. After 8 days of reactor operation, the removal of LAS in the reactor inoculated with active granular biomass exceeded the removal in the sterile reactor inoculated with sterile granular biomass. The effect of sorption ceased after 185 to 555 h depending on the LAS homologs. 40% of the LAS was biodegraded, and the removal rate was 0.5 x 10(-6) mol/h/mL granular biomass. Acidified effluent from the reactor was subjected to dichloromethane extraction followed by gas chromatography/mass spectrometry. Benzenesulfonic acid and benzaldehyde were detected in the reactor effluent from the reactor with active granular biomass but not in the sterile and unamended reactor effluent. Benzenesulfonic acid and benzaldehyde are the first identified degradation products in the anaerobic degradation of LAS.
Mogensen
A S
AS
The Environmental Microbiology and Biotechnology Group, BioCentrum-DTU, Building 227, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Ahring
B K
BK
D017738:Alkanesulfonic Acids / Q000378:metabolism*; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors; D012722:Sewage / Q000737:chemistry / Q000382:microbiology*; D013696:Temperature
D017738:Alkanesulfonic Acids; D012722:Sewage; C030641:alkylbenzyl sulfonic acid