title: Crystallization and preliminary X-ray crystallographic studies on acyl-(acyl carrier protein) from Escherichia coli.
abstract: Acyl carrier proteins carry the lipid substrate to the enzymes of the fatty acid synthase system. Crystals of Escherichia coli acyl carrier protein to which a butyryl group has been attached via a thioester link to the phosphopantetheine prosthetic arm have been obtained by the hanging-drop vapour-diffusion method. These crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 27.6, b = 41.6, c = 63.7 A. The asymmetric unit appears to contain one subunit, corresponding to a packing density of 2.1 A(3) Da(-1). Crystals of the selenomethionine-substituted (SeMet) protein were obtained using different conditions and belong to space group P6(3), with unit-cell parameters a = b = 63.4, c = 37.0 A, alpha = beta = 90, gamma = 120 degrees and with a monomer in the asymmetric unit (V(M) = 2.5 A(3) Da(-1)). Crystals of a SeMet butyryl-ACP I62M variant were obtained using the conditions for the native protein. Like the native crystals, these belong to space group P2(1)2(1)2(1) and have unit-cell parameters a = 27.3, b = 41.9, c = 64.5 A. A data set suitable for MAD phasing was collected from the crystals of the I62M variant to 1.8 A resolution on the ESRF beamline ID14-4.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England.
authors.forename: Anna
authors.initials: A
authors.lastname: Roujeinikova
authors.forename: Clair
authors.initials: C
authors.lastname: Baldock
authors.forename: William J
authors.initials: WJ
authors.lastname: Simon
authors.forename: John
authors.initials: J
authors.lastname: Gilroy
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Baker
authors.forename: Antoine R
authors.initials: AR
authors.lastname: Stuitje
authors.forename: David W
authors.initials: DW
authors.lastname: Rice
authors.forename: John B
authors.initials: JB
authors.lastname: Rafferty
authors.forename: Antoni R
authors.initials: AR
authors.lastname: Slabas
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D012645:Selenomethionine / Q000737:chemistry
chemical_list: D000213:Acyl Carrier Protein; D012645:Selenomethionine