[Study of protein and carbohydrate products, synthesized by Yersinia pestis under conditions imitating the internal environment of mammalian phagolysosomal macrophages].
Acid shift (pH 4.0) of liquid nutrient medium containing 20 mM Mg2+ created conditions in vitro simulating the internal environment of phagolysosome into which Yersinia pestis captured by a macrophage get in vivo. The capacity of Y. pestis to survive and multiply under these conditions irrespective of the plasmid composition of strains was confirmed experimentally. Y. pestis possesses a specific mechanism of fibrinolytic activity inhibition, preventing proteolytic degradation under the effect of Ca-dependent polypeptide (Yops) fibrinolysin and potentiating, in addition to these latter, the production of the so-called "acid" proteins by Y. pestis, coded for by pCad2+ or chromosome, including the potentially new members of LCR family. The culturing conditions affect the length of O-specific lateral chains of Y. pestis lipopolysaccharide (LPS), which corresponds to LPS SR, but not R form.
Fedorova
V A
VA
Russian Plague Control Institute Mikrob, Saratov.
Devdariani
Z L
ZL
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000096:biosynthesis / Q000737:chemistry; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D006868:Hydrolysis; D008247:Lysosomes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D015010:Yersinia pestis / Q000378:metabolism* / Q000472:pathogenicity
D001426:Bacterial Proteins; D002241:Carbohydrates; D005341:Fibrinolysin; D002118:Calcium