Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta.
Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.
Watanabe
Madoka
M
Department of Applied Biological Science, Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
Hanashima
Shinya
S
Mizushina
Yoshiyuki
Y
Yoshida
Hiromi
H
Oshige
Masahiko
M
Sakaguchi
Kengo
K
Sugawara
Fumio
F
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002846:Chromatography, Affinity; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D019951:DNA Polymerase beta / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D007202:Indicators and Reagents; D007700:Kinetics; D008095:Lithocholic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D008095:Lithocholic Acid; D001710:Biotin; D004256:DNA Polymerase I; D019951:DNA Polymerase beta