Lymphatic networks in the periodontal tissue and dental pulp as revealed by histochemical study.
The structural organization and fine distribution of the lymphatic networks in the periodontal tissues (gingiva, periodontal membrane, and alveolar process) and dental pulp of animals and humans were reviewed with special reference to histochemical examination by light and electron microscopy. The distinction between lymphatics and blood vessels was made on cryostat sections of undecalcified and calcified teeth treated with EDTA solution and whole mount preparations of periodontal membranes using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining. This staining procedure allowed lymphatic vessels in the periodontal tissue and dental pulp to be differentiated from blood vessels. The specificity and localization of the enzyme reactions were confirmed by comparative histochemical studies of the same specimen with light microscopy and scanning or transmission electron microscopy. Well-developed 5'-Nase-positive lymphatic networks were observed on the tissue sections and whole mount preparations of the gingiva, periodontium, and dental pulp. More lymphatic vessels were seen in the root area of the periodontium than in the cervical area. In the dental pulp, lymphatic vessels were more numerous in the central part than in the peripheral odontoblastic layer. These distributions of the lymphatic capillary networks are discussed in relation to their ability to supply lymph to the teeth.
Matsumoto
Yuushi
Y
Department of Oral and Maxillo-Facial Surgery, Oita Medical University, Oita 879-5593, Japan. ymatumot@oita-med.ac.jp
Zhang
Bi
B
Kato
Seiji
S
D015720:5'-Nucleotidase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D003782:Dental Pulp / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D008208:Lymphatic System / Q000648:ultrastructure*; D008251:Macaca; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D010519:Periodontium / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase