Investigation of substance P transport across the blood-brain barrier.
The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.
Freed
Anita L
AL
Department of Pharmaceutical Chemistry, The University of Kansas, School of Pharmacy, Lawrence KS 66047, USA. slunte@ku.edu
Audus
Kenneth L
KL
Lunte
Susan M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D001693:Biological Transport, Active; D001812:Blood-Brain Barrier; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003505:Cyclodextrins / Q000378:metabolism; D019075:Electrophoresis, Capillary; D004705:Endocytosis; D004730:Endothelium, Vascular / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D013373:Substance P / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D003505:Cyclodextrins; D007202:Indicators and Reagents; D009281:Naphthalenes; D013373:Substance P; C027717:2,3-naphthalenedicarboxaldehyde