title: The mast cell function-associated antigen and its interactions with the type I Fcepsilon receptor.
abstract: Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. The nature of MAFA-FcepsilonRI interactions giving rise to this inhibition remains unclear. Rotational diffusion of a membrane protein is a sensitive probe of its involvement in intermolecular interactions. We have therefore studied by time-resolved phosphorescence anisotropy the rotational behavior of both MAFA and FcepsilonRI as ligated by various reagents involved in FcepsilonRI-induced degranulation and MAFA-mediated inhibition thereof. From 4 to 37 degrees C, the rotational correlation times (mean +/- SD) of FcepsilonRI-bound, erythrosin-conjugated IgE resemble those observed for MAFA-bound, erythrosin-conjugated G63 Fab, 82 +/- 17 and 79 +/- 31 micros at 4 degrees C, respectively. Clustering the FcepsilonRI-IgE complex by antigen or by anti-IgE increases the phosphorescence anisotropy of G63 Fab and slows its rotational relaxation. Lateral diffusion of G63 Fab is also slowed by antigen clustering of the receptor. Taken together, these results indicate that unperturbed MAFA associates with clustered FcepsilonRI. They are also consistent with its interaction with the isolated receptor.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, and Department of Immunology, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Jinming
authors.initials: J
authors.lastname: Song
authors.forename: Guy M
authors.initials: GM
authors.lastname: Hagen
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Roess
authors.forename: Israel
authors.initials: I
authors.lastname: Pecht
authors.forename: B George
authors.initials: BG
authors.lastname: Barisas
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003432:Cross-Linking Reagents; D004923:Erythrosine / Q000378:metabolism; D007073:Immunoglobulin E / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007700:Kinetics; D037181:Lectins, C-Type; D008163:Luminescent Measurements; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D009092:Mucous Membrane / Q000276:immunology / Q000502:physiology; D051381:Rats; D017455:Receptors, IgE / Q000737:chemistry / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007140:Immunoglobulin Fab Fragments; C094696:Klrg1 protein, rat; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D017455:Receptors, IgE; D007073:Immunoglobulin E; D004923:Erythrosine