A P element transformation vector for high levels of gene expression in germ-line cells of the ovary and undifferentiated cells in the developing eye of Drosophila.
Many vectors are available for expression of cloned genes in the Drosophila germ line and eye. Nevertheless, for experiments that require female germ line expression, it is often problematic to find a vector that directs transcription suitably early and at high enough levels. In addition, a vector specific for undifferentiated cells in the Drosophila eye has yet to be described. Here, we have used the enhancer and promoter sequences of the fat facets gene to construct a P element transformation vector, pFAF, that provides high levels of gene expression in the female germ line. We present evidence that pFAF is a more potent activator of transcription in the female germ line than other commonly used vectors. In addition, in the eye, pFAF is unique in that it activates transcription specifically in undifferentiated cells.
Chen
Xin
X
Section of Molecular Cell and Developmental Biology, The Institute for Cellular and Molecular Biology, Austin, Texas 78712, USA.
Fischer
Janice A
JA
D000818:Animals; D002454:Cell Differentiation; D004251:DNA Transposable Elements; D029721:Drosophila Proteins / Q000235:genetics*; D005123:Eye / Q000166:cytology; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D005854:Germ Cells; D019476:Insect Proteins / Q000235:genetics*; D007763:Lac Operon; D010053:Ovary / Q000166:cytology*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D019476:Insect Proteins