Ethanol-induced hypogonadism is not dependent on activation of the hypothalamic-pituitary-adrenal axis.
The well-characterized suppression of the male reproductive unit after ethanol (EtOH) exposure has been speculated to be partially due to activation of the hypothalamic-pituitary-adrenal (HPA) axis. The subsequent corticosterone elevation could result in hypogonadism via suppression of hypothalamic LHRH, pituitary LH, or a direct gonadal effect. To directly examine this possibility, adult male Sprague Dawley rats were either adrenalectomized (ADX) or sham ADX. The ADX animals were given low dose corticosterone via replacement pellet, resulting in a steady level of serum corticosterone. The sham ADX (adrenal intact) animals were implanted with placebo pellets. Half of both groups were then exposed to EtOH by I.P. injection on two consecutive days to mimic an acute binge-drinking model. The other half was given saline I.P., serving as controls. In the adrenal intact animals, EtOH caused the expected rise in corticosterone, and fall in luteinizing hormone (LH) and testosterone. In the ADX animals, where constant levels of corticosterone were maintained by pellet implantation, EtOH resulted in similar LH and testosterone reduction. These results suggest that suppression of the reproductive axis is independent of the activation of the HPA unit.
Emanuele
N V
NV
Department of Medicine, Division of Research on Drugs of Abuse, Loyola University Medical Center, Maywood, IL 60153, USA. nemanue@wpo.it.lumc.edu
LaPaglia
N
N
Benefield
J
J
Emanuele
M A
MA
D000311:Adrenal Glands / Q000187:drug effects* / Q000503:physiopathology; D000315:Adrenalectomy; D000818:Animals; D003345:Corticosterone / Q000008:administration & dosage / Q000097:blood; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007006:Hypogonadism / Q000139:chemically induced* / Q000503:physiopathology; D007031:Hypothalamus / Q000187:drug effects* / Q000503:physiopathology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010902:Pituitary Gland / Q000187:drug effects* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood
D007987:Gonadotropin-Releasing Hormone; D000431:Ethanol; D013739:Testosterone; D007986:Luteinizing Hormone; D003345:Corticosterone