A simple formula useful for positional cloning.
We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and hence should be useful for planning positional cloning experiments. The predictions of this formula agree well with the results from a number of published experiments in Arabidopsis.
Durrett
Richard T
RT
Department of Mathematics, Cornell University, Ithaca, New York 14853-4201, USA.
Chen
Kai-Yi
KY
Tanksley
Steven D
SD
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005821:Genetic Techniques; D008957:Models, Genetic; D015233:Models, Statistical