[Expression of interleukin-18 in human leukemia cell line J6-1 and its significance].
OBJECTIVE
To elucidate the expression and regulation of interleukin-18 (IL-18) in human leukemia cell line J6-1, and investigate its possible role in leukemogenesis.


METHODS
IL-18 mRNA expression was detected using reverse transcriptase-ploymerase chain reaction (RT-PCR), and the specificity of IL-18 cDNA was verified by restriction endonucleases analysis. To sequence and analyze its cDNA alignment, a recombinant plasmid of IL-18 was constructed. Employing the antisense technique, we ionvestigated the effects of IL-18 antisense oligodeoxynucleiotide (ASODN) on the proliferation of J6-1 cells.


RESULTS
J6-1 cells constitutively expresses IL-18 mRNA, and its cDNA sequence shared 99% homology with the published IL-18 sequence,except that a synonymous point mutation (Codon35 UCA to UCC) was confirmed by sequencing and alignment analysis. Peripheral blood mononuclear cells (PBMC) of healthy volunteers weakly expresses IL-18 mRNA (0.13 +/- 0.05), while CpG-ODN significantly up-modulated IL-18 expression in PBMC (0.82 +/- 0.24), but not in J6-1 cells. Compared with K562, HL-60, U937 and LCL cells, IL-18 mRNA level expression of in J6-1 cells was the highest (0.98 +/- 0.29). ASODN of IL-18 markedly inhibited the proliferation of J6-1 cells (inhibition rate 43.3%), but did not show significant effects on HL-60 cells (inhibition rate 16.7%).


CONCLUSIONS
J6-1 cells constitutively expresses IL-18 mRNA, and no significant mutation was detected coding region of IL-18 cDNA. IL-18 might be a positive regulation factor for the proliferation of J6-1 cells after in an autocrine model.
Wang
Y
Y
Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin 300020, China.
Li
G
G
Rao
Q
Q
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D018899:CpG Islands / Q000235:genetics; D004252:DNA Mutational Analysis; D016373:DNA, Antisense / Q000494:pharmacology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D020382:Interleukin-18 / Q000235:genetics / Q000502:physiology*; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D009841:Oligonucleotides / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured; D020298:U937 Cells
D016373:DNA, Antisense; D018076:DNA, Complementary; D020382:Interleukin-18; D009841:Oligonucleotides; D012333:RNA, Messenger