Construction and synchronization of dnaA temperature-sensitive mutants of Streptomyces.
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39 degrees C replication in the mutants ceased in about 90 min but resumed on return to 30 degrees C. This allowed manipulations to achieve replication synchronization.
Lee
Li-Fong
LF
Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan.
Yeh
Shun-Hua
SH
Chen
Carton W
CW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria