title: Microdissection is essential for gene expression profiling of clinically resected cancer tissues.
abstract: The gene expression array method enables us to achieve expression profiling with thousands of genes. Clinically resected bulk cancer tissues, however, contain not only cancer cells but also stromal cells, which may affect gene expression profiling and hamper accurate analysis of the cancer cells per se. Therefore, a procedure for dissecting specific cells, such as laser capture microdissection, is neededfor the clinical application of a gene expression array. There has been no study actually comparing 2 gene expression profiles, one obtained using RNA extractedfrom cancer cells by laser capture microdissection and one obtained using RNA extractedfrom bulk cancer tissues. Wefirst demonstrated the difference in expression patterns between them, without any amplification procedures. In addition, differential expression analysis between tumor and nontumor tissue yielded quite different patterns between the 2 methods. We conclude that microdissection is essential for gene expression profiling of clinical specimens.
authors.affiliation: Department of Gynecology, Cancer Institute Hospital, Tokyo, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sugiyama
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hirai
authors.forename: Futoshi
authors.initials: F
authors.lastname: Akiyama
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hasumi
mesh_terms: D004210:Dissection; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology*; D012313:RNA / Q000032:analysis / Q000302:isolation & purification
chemical_list: D012313:RNA