Effect of flavonoids on cell cycle progression in prostate cancer cells.
The effect of some flavonoids, which are components of fruits, vegetables, and peas, on the cell cycle progression of human LNCaP prostate cancer cells has been investigated in this study. Genistein arrested the cell cycle at the G2/M phases, which is attributed to the suppression of cyclin B expression. In addition, genistein induced the cyclin-dependent kinase inhibitor p21, which does not depend on p53 activation. Apigenin and luteolin also increased p21 levels, but quercetin did not. Apigenin induced p21 production through a p53-dependent pathway, but luteolin did so in a p53-independent manner. These results suggest that flavonoids are potent regulators of cyclin B and p21 for cell cycle progression, which may play some roles in prevention of carcinogenesis.
Kobayashi
Takashi
T
Department of Biochemistry, Yamagata University School of Medicine, Yamagata 990-9585, Japan.
Nakata
Teruhiro
T
Kuzumaki
Takejiro
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D047310:Apigenin; D002453:Cell Cycle / Q000187:drug effects*; D019926:Cyclin B / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005100:Expectorants / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D005434:Flow Cytometry; D016195:G2 Phase; D016158:Genes, p53 / Q000235:genetics; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D047311:Luteolin; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis; D008956:Models, Chemical; D010766:Phosphorylation; D011233:Precipitin Tests; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D011794:Quercetin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D000970:Antineoplastic Agents; D019926:Cyclin B; D005100:Expectorants; D005419:Flavonoids; D016159:Tumor Suppressor Protein p53; D047310:Apigenin; D011794:Quercetin; D019833:Genistein; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D047311:Luteolin