title: A new strategy for the detection of subtelomeric rearrangements.
abstract: We present a new strategy for the detection of subtelomeric rearrangements. This approach is based on two hybridizations with different probe sets. The first set consists of microdissected subtelomeric probes (each 5-10 megabases in size) labeled combinatorially employing 7 different fluorochromes. With this set, subtelomeric interchromosomal exchanges can be detected in a 24-color experiment. The second set comprises a second generation of subtelomeric PAC-, P1- and BAC-clones. Probes for p- and q-arms are labeled with two different colors. This second set detects small deletions; in addition it provides regional information, so that translocated material identified by the first probe set can be assigned to the p- or q-arm of a chromosome. The test has been evaluated in a blind study on a series of subtle translocations and deletions.
authors.affiliation: Institut für Humangenetik, Technische Universität München, Trogerstr. 32, D-81675 München, Germany. fauth@humangenetik.med.tu-muenchen.de
authors.forename: C
authors.initials: C
authors.lastname: Fauth
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Harabacz
authors.forename: J
authors.initials: J
authors.lastname: Brown
authors.forename: K
authors.initials: K
authors.lastname: Saracoglu
authors.forename: G
authors.initials: G
authors.lastname: Lederer
authors.forename: O
authors.initials: O
authors.lastname: Rittinger
authors.forename: I
authors.initials: I
authors.lastname: Rost
authors.forename: R
authors.initials: R
authors.lastname: Eils
authors.forename: L
authors.initials: L
authors.lastname: Kearney
authors.forename: M R
authors.initials: MR
authors.lastname: Speicher
mesh_terms: D020223:Chromosome Painting; D002877:Chromosomes, Human / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D017384:Sequence Deletion; D016615:Telomere / Q000235:genetics*; D014178:Translocation, Genetic