Cross-linking of GPI-80, a possible regulatory molecule of cell adhesion, induces up-regulation of CD11b/CD18 expression on neutrophil surfaces and shedding of L-selectin.
Previously, we described a novel glycosylphosphatidyl inositol (GPI)-anchored glycoprotein (designated GPI-80) on human neutrophils and monocytes that may regulate beta(2) integrin-dependent neutrophil adherence and migration. However, the mechanism regulating beta(2) integrin remains to be clarified. To study this, we examined changes in beta(2) integrin expression and function caused by cross-linking GPI-80. GPI-80 cross-linking induced up-regulation of CD11b/CD18 (Mac-1) expression on neutrophil surfaces and shedding of L-selectin, which depends on tyrosine phosphorylation and cytoskeleton remodeling. Furthermore, the cross-linking enhanced fMLP-induced human neutrophil adherence. These results suggest that GPI-80 may be a regulator of beta(2) integrin in neutrophils.
Yoshitake
Hiroshi
H
Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata, Japan.
Takeda
Yuji
Y
Nitto
Takeaki
T
Sendo
Fujiro
F
D000581:Amidohydrolases; D000974:Antibodies, Antinuclear; D017152:Antibodies, Antiphospholipid; D018821:CD18 Antigens / Q000096:biosynthesis* / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000502:physiology*; D003432:Cross-Linking Reagents; D058851:GPI-Linked Proteins; D006801:Humans; D006867:Hydrolases; D066298:In Vitro Techniques; D019041:L-Selectin / Q000378:metabolism* / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000096:biosynthesis* / Q000502:physiology; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction; D015854:Up-Regulation / Q000502:physiology
C442826:3H9 protein, mouse; D000974:Antibodies, Antinuclear; D017152:Antibodies, Antiphospholipid; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents; D058851:GPI-Linked Proteins; D016177:Macrophage-1 Antigen; D019041:L-Selectin; D006867:Hydrolases; D000581:Amidohydrolases; C121771:VNN2 protein, human