title: Regulated gene expression in the chicken embryo by using replication-competent retroviral vectors.
abstract: Rous sarcoma virus (RSV)-derived retroviral vector could efficiently deliver the green fluorescent protein (GFP), which is driven by the internal cytomegalovirus enhancer/promoter, into restricted cell populations in the chicken embryo. RSV-derived vectors coupled with the tet regulatory elements also revealed doxycycline-dependent inducible GFP expression in the chicken embryo in ovo.
authors.affiliation: Department of Anatomy, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan. nsato@fmu.ac.jp
authors.forename: Noboru
authors.initials: N
authors.lastname: Sato
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsuda
authors.forename: Chie
authors.initials: C
authors.lastname: Sakuma
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Foster
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Oppenheim
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yaginuma
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000502:physiology; D002642:Chick Embryo; D003587:Cytomegalovirus / Q000235:genetics; D004318:Doxycycline / Q000494:pharmacology; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D000900:Anti-Bacterial Agents; D008164:Luminescent Proteins; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein; D049452:Green Fluorescent Proteins; D004318:Doxycycline