A novel expression cassette of lyssavirus shows that the distantly related Mokola virus can rescue a defective rabies virus genome.
By comparing three expression vectors for the rabies virus (Rv) minigenome, we show that the characteristic of the Rv RNA is important for efficient rescue despite its not being crucial for replication. Moreover, we show that the coexpression of the viral proteins from helper Rv and Mokola virus could rescue the Rv minigenome while Rv-related European bat lyssavirus 1 could not, suggesting that the signals controlling transcription and replication are conserved in the distantly related Rv and Mokola virus.
Le Mercier
Philippe
P
Laboratoire des Lyssavirus, Institut Pasteur, 75724 Paris Cédex 15, France. Philippe.LeMercier@medecine.unige.ch
Jacob
Yves
Y
Tanner
Kyle
K
Tordo
Noël
N
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics*; D005822:Genetic Vectors; D016679:Genome, Viral; D006378:Helper Viruses / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018114:Lyssavirus / Q000235:genetics* / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics; D011820:Rabies virus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D018076:DNA, Complementary; D016337:RNA, Catalytic; D012367:RNA, Viral; D014764:Viral Proteins