Separation of finasteride and analogues.
This review is focused on the different chromatographic strategies for determination of finasteride and its analogues in biological fluids. These compounds are used for the treatment of benign prostatic hyperplasia. Particular attention is paid to high-performance liquid chromatography with spectrophotometric and mass spectrometric detection, the clean-up procedures are also included. The relationships between pharmacokinetics of finasteride, dose administered and required limit of quantitation of the chromatographic assays are discussed. Tandem mass spectrometry is recommended as the detection method for measuring concentrations <1 ng/ml, while cheaper spectrophotometric detection may be selected for determination of higher concentrations.
Macek
J
J
Pharmakl spol. sro, Laboratory and Clinical Facility, U Vojenské Nemocnice, Prague, Czech Republic. pharmakl@mbox.vol.cz
D002853:Chromatography, Liquid / Q000379:methods; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics; D018120:Finasteride / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics
D004791:Enzyme Inhibitors; D018120:Finasteride