[Nested polymerase chain reaction in detecting Mycobacterium tuberculosis DNA in bioptic tissue from patients with endobronchial tuberculosis].
OBJECTIVE
To evaluate the nested polymerase chain reaction (NPCR) technique in diagnosing endobronchial tuberculosis.


METHODS
NPCR method was used to detect Mycobacterium tuberculosis DNA in bioptic tissue from 67 patients with endobronchial tuberculosis, and the results were compared with pathologic examination, brushing smear, sputum smear and culture for Mycobacterium tuberculosis after bronchoscopy. The controls were 43 patients with lung cancer.


RESULTS
The positive rates of detecting Mycobacterium tuberculosis were 13%, 19%, 22%, 15% and 76% respectively by pathologic examination, brushing smear, sputum smear, culture after bronchoscopy and NPCR methods. Significant differences were found between NPCR and the other 4 methods (all Ps < 0.01). No positive result for Mycobacterium tuberculosis was found in 43 controls by NPCR.


CONCLUSIONS
NPCR is a useful method for diagnosing patients with endobronchial tuberculosis, especially for those with normal demonstration in X-ray chest film, negative sputum and nonspecific pathologic changes in endobronchial biopsy.
Ma
L
L
The 460th Hospital of the PLA, Zhengzhou 450007.
Miao
L
L
Qin
S
S
Zhang
S
S
D001706:Biopsy; D001982:Bronchial Diseases / Q000175:diagnosis*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004269:DNA, Bacterial