[Transporting into the kidney of medications injected into the renal adipose capsules of rats].
OBJECTIVE
To study the possibility of transporting into the renal tissue of medications by intrarenal adipose capsule injection (IC).


METHODS
1. Methylene blue (MB) was injected into the renal adipose capsule of 18 male healthy SD rats, and normal saline was injected into the renal adipose capsule at the side as control. Both kidneys taken out 1 - 24 hours after the injection, frozen slices were made to observe the transport of MB. 2. Salvia miltiorrhizae (SM) was injected into both renal adipose capsule of 18 male healthy SD rats. 48 healthy male SD rats were randomly divided into two groups, Tetramethylpyrazine (20 mg) was injected into per renal adipose capsule of the rats in one group and 40 mg Tetramethylpyrazine (TP) was injected into the veins of the rats in another group. 64 SD rats were randomly divided into two groups, methylprednisolone sodium succinate (25 mg/kg) was injected into per renal adipose capsule of the rats in one group and 50 mg/kg methylprednisolone (MP) sodium succinate was injected into the veins of the rats in another group. At different time, both kidneys and blood samples of all the rats were taken out. The contents of the three medications inside renal tissue and its blood samples were individually detected by HPLC.


RESULTS
The peak values in the renal tissue of MB, SM, TP, MP were successively 6 h, 24 h (36 mg/g +/- 18 mg/g), 12 h (2 288 microgram/g +/- 236 microgram/g) and 1 h (120 microgram/g +/- 16 microgram/g) after IC. The medicine diffusing into the kidney can maintain longer time in IC group.


CONCLUSIONS
The renal adipose capsule can be acted as the place of medicine storing and releasing into the kidney. The medicine diffusing into the kidney can attain higher concentration and maintain longer time. IC is a feasible method of local renal ad ministration with much less side effects.
Cai
M
M
Renal Division, People's Hospital, Beijing University, Beijing 100044, China.
Yu
Z
Z
Tian
S
S
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D007267:Injections; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008751:Methylene Blue / Q000493:pharmacokinetics; D008775:Methylprednisolone / Q000493:pharmacokinetics; D011719:Pyrazines / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza
D011719:Pyrazines; D008751:Methylene Blue; C017953:tetramethylpyrazine; D008775:Methylprednisolone