title: PRINS, the other in situ DNA labeling method useful in cellular biology.
abstract: PRINS has proven to be an attractive alternative to FISH for in situ DNA labeling. PRINS is specific, simple, and rapid. We review some applications of PRINS involving primers specific for telomeric, human Alu, and centromeric alpha-satellite sequences. Bicolor labeling, PRINS-FISH, or PRINS-immunofluorescent combinations have been developed to enable investigations in numerous applications.
authors.affiliation: UMR1599 Génomique cellulaire des cancers, Villejuif, France. pcoullin@igr.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Coullin
authors.forename: Lydia
authors.initials: L
authors.lastname: Roy
authors.forename: Franck
authors.initials: F
authors.lastname: Pellestor
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Candelier
authors.forename: Bertrand
authors.initials: B
authors.lastname: Bed-Hom
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Guillier-Gencik
authors.forename: Alain
authors.initials: A
authors.lastname: Bernheim
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002869:Chromosome Aberrations; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D020401:Primed In Situ Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers