title: The function of Ca(2+) channel subtypes in exocytotic secretion: new perspectives from synaptic and non-synaptic release.
abstract: By mediating the Ca(2+) influx that triggers exocytotic fusion, Ca(2+) channels play a central role in a wide range of secretory processes. Ca(2+) channels consist of a complex of protein subunits, including an alpha(1) subunit that constitutes the voltage-dependent Ca(2+)-selective membrane pore, and a group of auxiliary subunits, including beta, gamma, and alpha(2)-delta subunits, which modulate channel properties such as inactivation and channel targeting. Subtypes of Ca(2+) channels are constituted by different combinations of alpha(1) subunits (of which 10 have been identified) and auxiliary subunits, particularly beta (of which 4 have been identified). Activity-secretion coupling is determined not only by the biophysical properties of the channels involved, but also by the relationship between channels and the exocytotic apparatus, which may differ between fast and slow types of secretion. Colocalization of Ca(2+) channels at sites of fast release may depend on biochemical interactions between channels and exocytotic proteins. The aim of this article is to review recent work on Ca(2+) channel structure and function in exocytotic secretion. We discuss Ca(2+) channel involvement in selected types of secretion, including central neurotransmission, endocrine and neuroendocrine secretion, and transmission at graded potential synapses. Several different Ca(2+) channel subtypes are involved in these types of secretion, and their function is likely to involve a variety of relationships with the exocytotic apparatus. Elucidating the relationship between Ca(2+) channel structure and function is central to our understanding of the fundamental process of exocytotic secretion.
authors.affiliation: Department of Physiology, University of Saskatchewan, 107 Wiggins Road, Sask., S7N 5E5, Saskatoon, Canada. thomas.fischer@usask.ca
authors.forename: T E
authors.initials: TE
authors.lastname: Fisher
authors.forename: C W
authors.initials: CW
authors.lastname: Bourque
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D005089:Exocytosis / Q000502:physiology*; D006198:Hair Cells, Auditory / Q000378:metabolism; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism; D009490:Neurosecretory Systems / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D021122:Protein Subunits; D013570:Synaptic Membranes / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; D018377:Neurotransmitter Agents; D021122:Protein Subunits