title: CD56+, NKp46+ cell line (MZ93) expressing T-cell and myeloid antigens.
abstract: The MZ93 cell line, established from a patient with CML, expressed CD4, CD7, CD13, CD25, CD33, CD34, CD56 and NKp46. The additional karyotype abnormality of the Ph-positive leukemia cells in vivo, 6p+, was also observed in MZ93. The early passages of MZ93 expressed CD3 in the cytoplasm, but the late passages did not. The cells did not express mature NK-markers as expected. The messenger RNAs of CD2 and NKp46 were detected and those of CD3varepsilon and CD3zeta were absent in the cells. Therefore, the cell line has the immunophenotype likely to NK and/or T cell precursor.
authors.affiliation: First Department of Internal Medicine, School of Medicine, Niigata University, Asahimachi-dori 1, Niigata City 951-8520, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Ken
authors.initials: K
authors.lastname: Toba
authors.forename: Junjiro
authors.initials: J
authors.lastname: Tsuchiyama
authors.forename: Takashi
authors.initials: T
authors.lastname: Abe
authors.forename: Toshio
authors.initials: T
authors.lastname: Yano
authors.forename: Akihito
authors.initials: A
authors.lastname: Momoi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okazuka
authors.forename: Naoko
authors.initials: N
authors.lastname: Kanazawa
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takahashi
authors.forename: Yoshifusa
authors.initials: Y
authors.lastname: Aizawa
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis*; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D019002:CD56 Antigen; C112260:NCR1 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic