title: Individual molecules of thermostable alkaline phosphatase support different catalytic rates at room temperature.
abstract: Thermus thermophilus cells were grown at 70 degrees C, lysed and the lysate subjected to single molecule alkaline phosphatase assays, using a capillary electrophoresis laser-induced fluorescence detection-based method. The enzyme was found to be heterogeneous with respect to catalytic rate when assayed at room temperature. Turnover numbers ranged 12-fold, with an average of 400 +/- 200 reactions/min for the 80 molecules assayed.
authors.affiliation: Department of Chemistry, University of Winnipeg, Winnipeg, MB R3B 2E9, Canada.
authors.forename: Ashley C
authors.initials: AC
authors.lastname: Dyck
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Craig
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism*; D002384:Catalysis; D019075:Electrophoresis, Capillary; D004795:Enzyme Stability; D006358:Hot Temperature; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D013329:Structure-Activity Relationship; D013696:Temperature; D016963:Thermus thermophilus / Q000201:enzymology / Q000254:growth & development
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase