title: Immunodiagnosis of the primary brain tumor (glioma) by the endogenous lectin.
abstract: BACKGROUND
  A sugar-binding protein, lectin, was identified in the cystic fluid of brain tumor (glioma).
  
  
  METHODS
  The protein was purified by high performance liquid chromatography.
  
  
  RESULTS
  The molecular weight of the lectin was 29 kDa. The activity of the purified protein was inhibited strongly by p-nitro-beta-D-galactose and asialofetuin. The lectin activity was not Ca(2+)- or Mg(2+)-dependent. The antiserum of this pure lectin specifically cross-reacted in the cerebrospinal fluid (CSF) of glioma tumor patients. The same antibody had no reaction with CSF of other neurological patients, normal rat brain tissue extract, oncofetal antigens such as alpha fetoprotein (AFP), carcinoembryonic antigen (CEA), plant lectins, and other body fluids like plural and ascites.
  
  
  CONCLUSIONS
  This antilectin antibody may be useful in the evaluation of patients with primary brain tumor (glioma).
authors.affiliation: Indian Institute of Chemical Biology, 4, Raja S.C. Mallick Road, 700 032, Calcutta, India. pranabbasu@iicb.res.in
authors.forename: Pranab S
authors.initials: PS
authors.lastname: Basu
authors.forename: Ramdhan
authors.initials: R
authors.lastname: Majhi
authors.forename: Samarendranath
authors.initials: S
authors.lastname: Ghosh
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Ghosh
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Batabyal
mesh_terms: D001826:Body Fluids / Q000276:immunology; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002272:Carcinoembryonic Antigen / Q000276:immunology; D016022:Case-Control Studies; D002555:Cerebrospinal Fluid / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D005910:Glioma / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D006801:Humans; D037102:Lectins / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D000509:alpha-Fetoproteins / Q000276:immunology
chemical_list: D002272:Carcinoembryonic Antigen; D037102:Lectins; D000509:alpha-Fetoproteins