title: A tridecapeptide possesses both antimicrobial and protease-inhibitory activities.
abstract: A 13-residue synthetic peptide (Rev4) was designed based on indolicidin, an antimicrobial peptide from bovine neutrophils. The synthetic peptide retains high antimicrobial activity. When tested for its stability in tobacco leaf extracts, Rev4 was highly stable compared to another antimicrobial peptide, magainin. When mixed with Rev4, magainin was protected from degradation by the leaf extract. Our results show that Rev4 is a potent protease inhibitor which selectively inhibits three out of four different types of proteases. Four other synthetic peptides were tested and the results were suggestive of no correlation between their antimicrobial and protease inhibitory activities.
authors.affiliation: Tobacco and Health Research Institute, University of Kentucky, Lexington, KY 40546-0236, USA. liq@muohio.edu
authors.forename: Qingshun
authors.initials: Q
authors.lastname: Li
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Lawrence
authors.forename: H
authors.initials: H
authors.lastname: Maelor Davies
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Everett
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D054806:Magainins; D009504:Neutrophils / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism; D029867:Xenopus Proteins
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D010455:Peptides; D011480:Protease Inhibitors; C451720:Rev4 peptide; D029867:Xenopus Proteins; C073143:indolicidin; C053301:magainin 2 peptide, Xenopus