title: Focal orchitis in undescended testes: discussion of pathogenetic mechanisms of tubular atrophy.
abstract: OBJECTIVE
  To evaluate seminiferous epithelium lesions in adult cryptorchid testes showing lymphoid infiltrates in seminiferous tubules and interstitium (i.e., focal orchitis). Also, to consider the possible role of this lesion in the etiology of tubular atrophy.
  
  
  METHODS
  We performed a histopathologic study of the cryptorchid testes and adjacent epididymides removed from 50 adult men who had not been previously treated for cryptorchidism. The study included morphologic and semiquantitative evaluation of seminiferous tubule pathology (according to germ cell numbers), Sertoli cell morphology, tubular lumen dilation, rete testis pattern (normal, hypoplastic, or cystic), and epididymal pattern (normal or epididymal duct hypoplasia). The study also included immunohistochemical evaluation of immune cell markers. The results were compared with clinical and laboratory findings.
  
  
  RESULTS
  Focal lymphoid infiltrates (mainly lymphocytes) in seminiferous tubules and interstitium were found in 22 patients (44%), all of whom had unilateral cryptorchidism. The course of orchitis was asymptomatic, and laboratory data were normal. According to the seminiferous tubule pathology, a variety of histopathologic diagnoses, were made: (1) mixed atrophy consisting of Sertoli cell-only tubules intermingled with tubules showing maturation arrest of spermatogonia (11 testes, 4 of which also showed hyalinized tubules); (2) Sertoli cell-only tubules plus hyalinized tubules (4 testes); (3) Sertoli cell-only tubules (3 testes); (4) intratubular germ cell neoplasia (2 testes, 1 of which also showed hyalinized tubules); (5) complete tubular hyalinization (1 testis); and (6) tubular hyalinization plus some groups of tubules with hypospermatogenesis (all germ cell types were present although in lower numbers, 1 testis). Dysgenetic Sertoli cells, that is, Sertoli cells that had undergone anomalous, incomplete maturation, were observed in all nonhyalinized seminiferous tubules with inflammatory infiltrates. Tubular ectasia was observed in 13 cases. The rete testis was hypoplastic and showed cystic transformation in 18 testes, and the epididymis was hypoplastic in 15 testes.
  
  
  CONCLUSIONS
  The causes of these focal inflammatory infiltrates are unknown. It is possible that tubular ectasia and Sertoli cell dysgenesis are involved and that these alterations cause a disruption of the blood-testis barrier and allow antigens to enter the testicular interstitium, giving rise to an autoimmune process.
authors.affiliation: Department of Morphology, School of Medicine, Autonomous University, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Nistal
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Riestra
authors.forename: Ricardo
authors.initials: R
authors.lastname: Paniagua
mesh_terms: D000328:Adult; D015703:Antigens, CD; D001284:Atrophy; D003456:Cryptorchidism / Q000150:complications / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009920:Orchitis / Q000150:complications / Q000276:immunology / Q000473:pathology*; D012671:Seminiferous Tubules / Q000276:immunology / Q000473:pathology*
chemical_list: D015703:Antigens, CD