title: Some tumors of the bladder are polyclonal in origin.
abstract: OBJECTIVE
  Clonality assays can be used to address different questions of neoplasm development. The study of the X chromosome inactivation pattern in female patients provides a useful and most commonly used indirect approach to demonstrate the monoclonal status of a cell population. We used this approach to examine whether recurrent tumors of the bladder supposed to be of monoclonal origin derive from different, independently transformed cells or whether they arise from 1 primary tumor.
  
  
  METHODS
  We analyzed 45 archival or fresh frozen bladder tumors from 27 female patients. We assessed the X inactivation status of each tumor by a polymerase chain reaction based method after HpaII digestion.
  
  
  RESULTS
  Surprisingly 16 of the 45 tumors revealed a polyclonal pattern. The amount of undigested DNA far exceeded what was explained by contamination with normal cells, as determined on histological sections indicating that these tumors were in fact polyclonal. This polyclonal status was further confirmed by a comprehensive series of controls.
  
  
  CONCLUSIONS
  Some bladder tumors are polyclonal in origin. Our findings are at variance with earlier observations and possibilities for explanation are proposed.
authors.affiliation: Department of Urology, University of Ulm, Ulm, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Paiss
authors.forename: Gudrun
authors.initials: G
authors.lastname: Wöhr
authors.forename: Richard E
authors.initials: RE
authors.lastname: Hautmann
authors.forename: Torsten
authors.initials: T
authors.lastname: Mattfeldt
authors.forename: Markus
authors.initials: M
authors.lastname: Müller
authors.forename: Juergen
authors.initials: J
authors.lastname: Haeussler
authors.forename: Walther
authors.initials: W
authors.lastname: Vogel
mesh_terms: D019175:DNA Methylation; D004273:DNA, Neoplasm; D004303:Dosage Compensation, Genetic; D005260:Female; D006801:Humans; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011944:Receptors, Androgen / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D011944:Receptors, Androgen