Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection.
A sensitive assay method was developed to determine fentanyl, an opiate agonist, in rat plasma by gas chromatography with nitrogen-phosphorus detection. For the pretreatment of plasma samples, sodium hydroxide was added to denature protein and n-butyl chloride was used to extract fentanyl. The calibration curve was linear within the concentration range 0.5 to 50 ng/ml (r=0.9997). The limit of detection was 0.1 ng/ml, and 0.5 ng/ml could be quantified with acceptable precision. Furthermore, fentanyl could be determined in only 200 microl of rat plasma. The method has been successfully applied to an intramuscular pharmacokinetic study at a dose of 10 microg/kg. Therefore, the current method is a valuable analytical tool for investigating the pharmacokinetics of fentanyl at low clinical doses.
Choi
H S
HS
Department of Polymer Science and Technology, Chonbuk National University, Chonju, South Korea.
Shin
H C
HC
Khang
G
G
Rhee
J M
JM
Lee
H B
HB
D000701:Analgesics, Opioid / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005283:Fentanyl / Q000097:blood* / Q000493:pharmacokinetics; D009584:Nitrogen; D010758:Phosphorus; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000701:Analgesics, Opioid; D010758:Phosphorus; D009584:Nitrogen; D005283:Fentanyl