Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.
Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
Rohdich
Felix
F
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany. felix.rodich@ch.tum.de
Hecht
Stefan
S
Gärtner
Katrin
K
Adam
Petra
P
Krieger
Cornelia
C
Amslinger
Sabine
S
Arigoni
Duilio
D
Bacher
Adelbert
A
Eisenreich
Wolfgang
W
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004755:Enterobacteriaceae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D009682:Magnetic Resonance Spectroscopy; D008798:Mevalonic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009876:Operon; D010088:Oxidoreductases; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D013729:Terpenes; D010088:Oxidoreductases; C109866:ispH protein, E coli; D008798:Mevalonic Acid