[The expression of Mdm2 protein and p53 mutation in lung squamous cell carcinomas and their clinicopathological significance].
OBJECTIVE
In order to study the interrelation between p53 mutation, Mdm2 overexpression and pulmonary squamous cell carcinomas.


METHODS
Forty-five pulmonary squamous cell carcinomas were investigated for expression of Mdm2 protein and p53 mutation using immunohistochemical techniques and PCR-SSCP. The findings were correlated with the clinicopathological features of carcinomas.


RESULTS
(1) Mdm2 protein overexpression (62%, 28/45) and p53 mutation (51%, 23/45) are a common event in pulmonary squamous cell carcinomas (P < 0.05). (2) Statistical analysis revealed a strong correlation between Mdm2/p53 coexpression and lymph metastases (66%, 10/15) was observed (P < 0.05).


CONCLUSIONS
The coexistence of Mdm2 protein and p53 aberration in a subset of pulmonary carcinomas may be indicative of a gain of function phenotype with more aggressive characteristic.
Zhang
J
J
Department of Pathology, Peking Union Medical college Hospital, Chinese Academy of Medical Sciense, Peking Union Medical College, Beijing 100730.
Liu
H
H
Li
S
S
Gu
C
C
D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D008207:Lymphatic Metastasis; D009154:Mutation; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D051736:Proto-Oncogene Proteins c-mdm2; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2