title: New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat.
abstract: Here we report a novel method of supplying cultured neurosphere cells to the injured spinal cord, by injection of cells into the cerebrospinal fluid (CSF) through the fourth ventricle or cisterna magna. Hippocampus-derived neurosphere cells, isolated from a transgenic rat fetus expressing green fluorescent protein, were transplanted into the CSF of a rat with spinal cord injury. It was found that injected cells were extensively transported by CSF within the subarachnoidal space, and survived as clusters on the pial surface of the spinal cord. The most notable finding was that a large number of injected cells migrated into the lesion site and integrated into the injured spinal cord tissues.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Sufan
authors.initials: S
authors.lastname: Wu
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kitada
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kataoka
authors.forename: Miyako
authors.initials: M
authors.lastname: Kitaura
authors.forename: Hirotomi
authors.initials: H
authors.lastname: Chou
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Chizuka
authors.initials: C
authors.lastname: Ide
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D016380:Brain Tissue Transplantation / Q000379:methods*; D002465:Cell Movement / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000502:physiology*; D005333:Fetus; D020546:Fourth Ventricle / Q000502:physiology / Q000601:surgery*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006085:Graft Survival / Q000502:physiology; D049452:Green Fluorescent Proteins; D006624:Hippocampus / Q000637:transplantation; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D009416:Nerve Regeneration / Q000502:physiology; D010841:Pia Mater / Q000166:cytology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D013119:Spinal Cord Injuries / Q000628:therapy*; D033581:Stem Cell Transplantation; D013346:Subarachnoid Space / Q000502:physiology / Q000601:surgery*; D014404:Tubulin / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins