A new strategy for the detection of subtelomeric rearrangements.
We present a new strategy for the detection of subtelomeric rearrangements. This approach is based on two hybridizations with different probe sets. The first set consists of microdissected subtelomeric probes (each 5-10 megabases in size) labeled combinatorially employing 7 different fluorochromes. With this set, subtelomeric interchromosomal exchanges can be detected in a 24-color experiment. The second set comprises a second generation of subtelomeric PAC-, P1- and BAC-clones. Probes for p- and q-arms are labeled with two different colors. This second set detects small deletions; in addition it provides regional information, so that translocated material identified by the first probe set can be assigned to the p- or q-arm of a chromosome. The test has been evaluated in a blind study on a series of subtle translocations and deletions.
Fauth
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C
Institut für Humangenetik, Technische Universität München, Trogerstr. 32, D-81675 München, Germany. fauth@humangenetik.med.tu-muenchen.de
Zhang
H
H
Harabacz
S
S
Brown
J
J
Saracoglu
K
K
Lederer
G
G
Rittinger
O
O
Rost
I
I
Eils
R
R
Kearney
L
L
Speicher
M R
MR
D020223:Chromosome Painting; D002877:Chromosomes, Human / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D017384:Sequence Deletion; D016615:Telomere / Q000235:genetics*; D014178:Translocation, Genetic