Modulation of remote DNA oxidation by hybridization with peptide nucleic acids (PNA).
We have examined the efficiency of DNA photooxidation in DNA/PNA duplex and DNA/(PNA)(2) triplex for the first time. DNA/PNA duplex was cleaved at GG steps by external riboflavin with high efficiency like specific GG cleavage in DNA/DNA duplex. However, the 5'G selectivity of the GG oxidation in DNA/PNA duplex was much lower than that observed in DNA/DNA duplex. Remote DNA oxidation of oxidant-tethered DNA/PNA duplex was considerably suppressed. In contrast, the formation of DNA/(PNA)(2) triplex by hybridization with two PNA strands completely inhibited the remote GG oxidation, indicating that PNA acts as an inhibition for remote oxidative DNA damage.
Okamoto
Akimitsu
A
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Tanabe
Kazuhito
K
Dohno
Chikara
C
Saito
Isao
I
D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D015226:Dinucleoside Phosphates / Q000378:metabolism / Q000528:radiation effects; D008958:Models, Molecular; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000528:radiation effects; D010083:Oxidants, Photochemical / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D010777:Photochemistry; D012256:Riboflavin / Q000494:pharmacology
D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D010083:Oxidants, Photochemical; D020135:Peptide Nucleic Acids; C015139:diguanosine triphosphate; D004247:DNA; D012256:Riboflavin