Control of smooth muscle cell function by membrane-type matrix metalloproteinases.
Vascular smooth muscle cells (SMCs) express membrane-type matrix metalloproteinases-1 and -3 (MT1- and MT3-MMPs). Expression is induced by PDGF in culture or by balloon injury in rat carotid arteries. In this study, we tried to define their functions in SMCs by transducing MT1- and MT3-MMP cDNAs into baboon-cultured SMCs, using adenoviral vectors. Overexpression of MT1-MMP increased the conversion of proMMP-2 to the activated form. In contrast, in MT3-MMP overexpressing cells, MMP-2 activation was partial. However, both MT1- and MT3-MMP overexpression elicited morphological alterations (cell rounding), which was prevented by BB94 addition. The cells, which underwent this change, showed reduced adhesion to matrices and increased migration in a Boyden chamber.
Shofuda
K I
KI
Department of Surgery, University of Washington School of Medicine, Seattle 98195-6410, USA.
Hasenstab
D
D
Shofuda
T
T
Kenagy
R D
RD
Clowes
A W
AW
D000818:Animals; D002339:Carotid Arteries / Q000503:physiopathology*; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D004789:Enzyme Activation; D005822:Genetic Vectors; D007700:Kinetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010215:Papio; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D014161:Transduction, Genetic
D010982:Platelet-Derived Growth Factor; D020782:Matrix Metalloproteinases; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2