Previously unknown and phylogenetically diverse members of the green nonsulfur bacteria are indigenous to freshwater lakes.
The phylogenetic diversity of green nonsulfur bacteria in nine stratified freshwater lakes was investigated. A set of oligonucleotide primers was developed that permitted the selective amplification of 16S rRNA gene sequences of this group. Subsequently, amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced, which yielded a total of 19 novel sequence types. Ten of the sequences were related to those of different cultivated members of the C hloroflexus assemblage, whereas nine fell into the T78 group of environmental clones. For the latter subgroup of the green nonsulfur bacteria, no molecular isolate from freshwater plankton has been reported so far. Several of the sequence types occurred in more than one lake, indicating that not only relatives of the C hloroflexus assemblage, but also bacteria of the clone T78 group represent indigenous bacteria of nonthermal stratified freshwater ecosystems. Our results indicate that the natural diversity in the phylum of the green nonsulfur bacteria has been significantly underestimated in the past.
Gich
F
F
Institute of Aquatic Ecology, University of Girona, Campus de Montilivi, 17071 Girona, Spain.
Garcia-Gil
J
J
Overmann
J
J
D001419:Bacteria / Q000145:classification / Q000235:genetics; D015373:Bacterial Typing Techniques; D001429:Bacteriochlorophylls / Q000378:metabolism; D019414:Chlorobi / Q000145:classification* / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D017753:Ecosystem; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001429:Bacteriochlorophylls; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S