title: Genes expressed during the IFN gamma-induced maturation of pre-B cells.
abstract: Interferon-gamma (IFN gamma) exerts diverse responses in B cell development ranging from growth arrest and apoptosis to proliferation and differentiation. IFN gamma stimulates murine 70Z/3 pre-B cells to express surface immunoglobulin (Ig) and this system serves as a useful model for the pre-B to immature B cell transition in B cell development. To analyze this developmental transition, we used a PCR-based subtractive hybridization in combination with miniarray screening to identify differentially-expressed genes in IFN gamma-stimulated compared with unstimulated 70Z/3 pre-B cells. The majority (44%) of the differentially-expressed genes obtained were known IFN gamma-inducible. These included multiple isolates from each of three multi-gene families, including two guanylate-binding protein (47 and 67kDa GBP) families of GTPases and the hematopoietic IFN gamma-inducible nuclear protein family (HIN-200). These multiple isolates of genes comprised the majority of the total isolated and sequenced clones. Other known IFN gamma-induced genes in this group included Ig kappa light chain and Ly-6, as well as genes with functions in antigen processing, cellular regulation, and cytoskeletal organization. Another 36% of the genes identified were previously known, but not known to be IFN gamma-inducible (e.g. pre-B cell enhancing factor, PBEF). The remaining 20% of the IFN gamma-induced isolates did not match entries in Genbank, and thus, may represent novel genes involved in IFN gamma responses and/or in the pre-B to immature B cell transition. Overall, the majority of the individual genes isolated were either not known to be IFN gamma responsive or were not previously known.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Patrone
authors.forename: Michael A
authors.initials: MA
authors.lastname: Damore
authors.forename: Michael B
authors.initials: MB
authors.lastname: Lee
authors.forename: Cindy Sue
authors.initials: CS
authors.lastname: Malone
authors.forename: Randolph
authors.initials: R
authors.lastname: Wall
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002454:Cell Differentiation; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D013234:Stem Cells / Q000187:drug effects; D015533:Transcriptional Activation
chemical_list: D012333:RNA, Messenger; D007371:Interferon-gamma; D019204:GTP-Binding Proteins