[Expression of transforming growth factor-beta 1 mRNA during mouse molar morphogenesis].
OBJECTIVE
To investigate the temporal and spatial expression and localization of transforming growth factor-beta 1 mRNA during mouse molar morphogenesis.


METHODS
The distribution pattern of TGF-beta 1 mRNA was analysed on 5 microns serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles using in situ hybridization.


RESULTS
The results show that TGF-beta 1 mRNA was expressed during mouse molar morphogenesis in a temporally and spatially regulated fashion. Local expression of TGF-beta 1 mRNA in the dental epithelium at bud-staged (E13) and cap-staged (E15) teeth were observed. During bell stage (E16-18), TGF-beta 1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF-beta 1 was increased in the layer of odontoblast and ameloblast with the differentiation of these cells.


CONCLUSIONS
These results suggest that TGF-beta 1 may have an important role in tooth morphogenesis, and TGF-beta 1 acts as a paracrine and autoinducing factor.
Zhu
Q
Q
Department of Endodontics, College of Stomatology, Wuhan University, Wuhan 430079, China.
Fan
M
M
Bian
Z
Z
D000818:Animals; D051379:Mice; D008963:Molar / Q000196:embryology* / Q000378:metabolism; D009024:Morphogenesis; D012333:RNA, Messenger / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000235:genetics*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta