title: Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration.
abstract: We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca(2+)](i) and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCepsilon to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.
authors.affiliation: Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, USA.
authors.forename: Doug
authors.initials: D
authors.lastname: Throckmorton
authors.forename: Doris
authors.initials: D
authors.lastname: Kurscheid-Reich
authors.forename: Oscar R
authors.initials: OR
authors.lastname: Rosales
authors.forename: Jose
authors.initials: J
authors.lastname: Rodriguez-Commes
authors.forename: Raquel
authors.initials: R
authors.lastname: Lopez
authors.forename: Bauer
authors.initials: B
authors.lastname: Sumpio
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhong
authors.forename: Ke Hong
authors.initials: KH
authors.lastname: Ding
authors.forename: Richard
authors.initials: R
authors.lastname: McCarthy
authors.forename: Paula Q
authors.initials: PQ
authors.lastname: Barrett
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Isales
mesh_terms: D000331:Aequorin / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002460:Cell Line; D002518:Ceramides / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010281:Parathyroid Hormone / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000096:biosynthesis / Q000737:chemistry; D021381:Protein Transport; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D015220:Calcium Channels; D002518:Ceramides; D004075:Diglycerides; D010281:Parathyroid Hormone; D010455:Peptides; D010743:Phospholipids; D020033:Protein Isoforms; D001464:Barium; D000331:Aequorin; D011493:Protein Kinase C; D011188:Potassium; D002118:Calcium