Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus.
White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST-P22). Temporal analysis showed that the p22 gene was a late gene. After binding between purified WSSV virions and anti-GST-P22 IgG followed by labelling with gold-labelled secondary antibody, the gold particles, under a transmission electron microscope, could be found along the outer envelope of WSSV virions. This experiment suggests that the p22 gene encodes an envelope protein of the virus.
Zhang
Xiaobo
X
Department of Biological Sciences and Tropical Marine Science Institute, National University of Singapore, Singapore1192601.
Huang
Canhua
C
Department of Biological Sciences and Tropical Marine Science Institute, National University of Singapore, Singapore1192601.
Xu
Xun
X
Key Laboratory of Marine Biotechnology, The Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, The People's Republic of China2.
Hew
Choy L
CL
Department of Biological Sciences and Tropical Marine Science Institute, National University of Singapore, Singapore1192601.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004267:DNA Viruses / Q000235:genetics / Q000378:metabolism*; D033364:Decapoda / Q000821:virology*; D015723:Gene Library; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D014759:Viral Envelope Proteins; C448664:p22 protein, Shrimp white spot syndrome virus