[The biological effects of transforming growth factor-beta on human periodontal ligament fibroblasts].
OBJECTIVE
To evaluate the biological effects of transforming growth factor-beta (TGF-beta) on human periodontal ligament fibroblasts (HPDLFs).


METHODS
HPDLFs were primary cultured and examined for the effects of TGF-beta on its proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.


RESULTS
TGF-beta(0.0050-0.1000 mg/L) significantly stimulated the proliferation of HPDLFs. The ALP activity of HPDLFs was elevated evidently by 0.0050 mg/L TGF-beta. TGF-beta (0.0005-0.1000 mg/L) had no effects on OC synthesis and formation of the mineralized nodules of HPDLFs.


CONCLUSIONS
The effects of TGF-beta on HPDLFs are dose-dependent. TGF-beta can stimulate HPDLFs to express some of osteoblastic phenotype, and it lacks the abilility to promote maturation of the osteogenic phenotype.
Si
X
X
Department of Medicine, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Liu
Z
Z
D000469:Alkaline Phosphatase / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta; D000469:Alkaline Phosphatase