Ovariectomy hinders the early stage of bone-implant integration: histomorphometric, biomechanical, and molecular analyses.
Postmenopausal osteoporosis is a contributing factor to alveolar bone atrophy associated with tooth loss in the elderly. The use of dental titanium implants has been increasingly adapted to treat these edentulous patients. This study examines whether female gonadal hormone deficiency interferes with the critical integration process between bone and implants. Two types of experimental titanium implants with acid-treated surfaces were placed in the femurs of ovariectomized (ovx) and sham-operated control rats: T-cell implants with a hollow chamber for histomorphometric and steady-state mRNA expression assays, and unthreaded cylindrical implants for biomechanical push-in tests. At week 2, less bone area was found in the ovx-implant group (p = 0.0495) than in the sham-implant group. The implant push-in test showed that the ovx-implant group had approximately half of the withstanding value of the sham-implant group (p = 0.009). However, these differences between the ovx and sham groups became diminished at week 4. Total RNA samples were examined by a reverse transcriptase-polymerase chain reaction assay for col1a1, col3a1, bone sialoprotein (bSP) II, osteonectin, osteopontin, osteocalcin, integrin beta1 and integrin beta3. In untreated bones and in created bone defects without implant placement, ovx did not affect the steady-state levels of the mRNAs tested. When implants were placed, significant upregulation of these genes was observed in the sham-implant group; however, only osteocalcin and integrins were upregulated in the ovx-implant group. The results suggest a biphasic effect of female gonadal hormone deficiency that may temporarily interfere with the early implant-tissue integration process, and which may be associated with a failure to upregulate a selected set of bone extracellular matrix genes. Once established, however, functional bone-implant integration can be achieved even in ovx rats.
Ozawa
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S
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials, and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA.
Ogawa
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T
Iida
K
K
Sukotjo
C
C
Hasegawa
H
H
Nishimura
R D
RD
Nishimura
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I
D000818:Animals; D001696:Biomechanical Phenomena; D001857:Bone Matrix / Q000378:metabolism; D015921:Dental Implants; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D016348:Osseointegration / Q000235:genetics / Q000502:physiology*; D010052:Ovariectomy / Q000009:adverse effects*; D019736:Prostheses and Implants; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014025:Titanium
D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D014025:Titanium