The association between PPP1R3 gene polymorphisms and type 2 diabetes mellitus.
OBJECTIVE
To detect the relationship between the polymorphism of the glycogen-targeting regulatory subunit of the skeletal muscle glycogen-associated protein phosphatase 1 (PPP1R3) gene and type 2 diabetes by case-control study.


METHODS
We genotyped the PPP1R3 gene Asp905Tyr polymorphism and a common 3'-untranslated region AT (AU)-rich element (ARE) polymorphism in 101 type 2 diabetic patients and 101 controls by oligonucleotide ligation assay (OLA) and polyacrylamide gel elecrophoresis, respectively.


RESULTS
Subjects with Tyr/Tyr genotypes whose body mass index (BMI) < 25 were used as the reference group. Those whose BMI > or = 25 with Asp905 had a 3.66-fold increase (95% CI: 1.48-9.06, P = 0.005) in type 2 diabetes risk. No association was found between 3'UTR ARE polymorphism and type 2 diabetes mellitus (OR = 1.15; 95% CI: 0.62-2.14, P = 0.65).


CONCLUSIONS
A joint effect between the Asp905 and BMI increases the risk of type 2 diabetes, and Asp905Tyr and ARE polymorphism of PPP1R3 gene are not the major diabetogenic gene variants in Chinese population.
Wang
G
G
Department of Endocrinology, Third Affiliated Hospital of Beijing University, Beijing 100083, China. wanggy88@hotmail.com
Qian
R
R
Li
Q
Q
Niu
T
T
Chen
C
C
Xu
X
X
D020856:AT Rich Sequence / Q000235:genetics; D000368:Aged; D000483:Alleles; D015992:Body Mass Index; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D011110:Polymorphism, Genetic; D054645:Protein Phosphatase 1
D004247:DNA; C114205:PPP1R3A protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1