Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment.
Type I allergy is an immunoglobulin E (IgE)-mediated hypersensitivity disease affecting more than 25% of the population. Currently, diagnosis of allergy is performed by provocation testing and IgE serology using allergen extracts. This process defines allergen-containing sources but cannot identify the disease-eliciting allergenic molecules. We have applied microarray technology to develop a miniaturized allergy test containing 94 purified allergen molecules that represent the most common allergen sources. The allergen microarray allows the determination and monitoring of allergic patients' IgE reactivity profiles to large numbers of disease-causing allergens by using single measurements and minute amounts of serum. This method may change established practice in allergy diagnosis, prevention, and therapy. In addition, microarrayed antigens may be applied to the diagnosis of autoimmune and infectious diseases.
Hiller
Reinhard
R
VBC-GENOMICS, A-1030 Vienna, Austria.
Laffer
Sylvia
S
Harwanegg
Christian
C
Huber
Martin
M
Schmidt
Wolfgang M
WM
Twardosz
Anna
A
Barletta
Bianca
B
Becker
Wolf M
WM
Blaser
Kurt
K
Breiteneder
Heimo
H
Chapman
Martin
M
Crameri
Reto
R
Duchêne
Michael
M
Ferreira
Fatima
F
Fiebig
Helmut
H
Hoffmann-Sommergruber
Karin
K
King
Te Piao
TP
Kleber-Janke
Tamara
T
Kurup
Viswanath P
VP
Lehrer
Samuel B
SB
Lidholm
Jonas
J
Müller
Ulrich
U
Pini
Carlo
C
Reese
Gerald
G
Scheiner
Otto
O
Scheynius
Annika
A
Shen
Horng-Der
HD
Spitzauer
Susanne
S
Suck
Roland
R
Swoboda
Ines
I
Thomas
Wayne
W
Tinghino
Raffaela
R
Van Hage-Hamsten
Marianne
M
Virtanen
Tuomas
T
Kraft
Dietrich
D
Müller
Manfred W
MW
Valenta
Rudolf
R
D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D007073:Immunoglobulin E / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
D000485:Allergens; D011994:Recombinant Proteins; D007073:Immunoglobulin E