[Genotyping of multiple genetic alterations of intrahepatic cholangiocarcinoma].
OBJECTIVE
To provide genetic evidence for understanding the mechanism of oncogenesis of intrahepatic cholangiocarcinoma (ICC) by detecting the multiple genetic spectrums.


METHODS
Twenty-two cases of paraffin-embedded ICC tissue sections were microdissected and genotyped by PCR-based DNA sequencing to detect the frequencies of loss of heterozygosity (LOH) of tumor suppressor genes APC, MCC, DCC, OGG1, p53, and RB1 and of point mutation of Ki-ras-2 oncogene.


RESULTS
The genetic alteration rate was 68.8% for APC, 46.2% for DCC, 41.7% for OGG1, 37.5% for p53, 27.3% for Ki-ras-2, 22.2% for RB1, and 14.3% for MCC.


CONCLUSIONS
ICC-related genetic spectrum is basically composed of APC, DCC, OGG1, p53, and Ki-ras-2. Multiple genetic alteration plays an important role in the carcinogenesis of ICC.
Cong
W
W
Oriental Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
Wu
M
M
Chen
H
H
D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001650:Bile Duct Neoplasms / Q000235:genetics / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000235:genetics; D018281:Cholangiocarcinoma / Q000235:genetics / Q000473:pathology*; D000075389:DCC Receptor; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D043382:DNA-Formamidopyrimidine Glycosylase; D005260:Female; D011905:Genes, ras / Q000235:genetics; D005838:Genotype; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D011956:Receptors, Cell Surface; D016160:Retinoblastoma Protein / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics
D025601:Adenomatous Polyposis Coli Protein; D015815:Cell Adhesion Molecules; D000075389:DCC Receptor; C501909:DCC protein, human; D004273:DNA, Neoplasm; D011956:Receptors, Cell Surface; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C067857:MCC protein, human; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase