Extraction and LC determination of lysine clonixinate salt in water/oil microemulsions.
A new reversed-phase high performance liquid chromatography method has been developed and validated for the quantitative determination of lysine clonixinate salt in water/oil microemulsions. The mobile phase was acetonitrile-buffer phosphate pH 3.3. Detection was UV absorbance at 252 nm. The precision and accurately of the method were excellent. The established linearity range was 5-60 microg ml(-1) (r(2)=0.999). Microemulsions samples were dispersed with chloroform and extracted lysine clonixinate salt with water. This easy method employing chloroformic extraction has been done three times. The recovery of lysine clonixinate salt from spiked placebo and microemulsion were >90% over the linear range.
Pineros
I
I
Dpto. Farmacia y Tecnologia Farmaceutica, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain. ipineros@eucmax.sim.ucm.es
Ballesteros
P
P
Lastres
J L
JL
D000700:Analgesics / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003002:Clonixin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D004655:Emulsions; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D009821:Oils / Q000032:analysis*; D014867:Water / Q000032:analysis*
D000700:Analgesics; D004655:Emulsions; D009821:Oils; D014867:Water; C089163:lysine clonixinate; D008239:Lysine; D003002:Clonixin