title: Effects of anthelmintics on the development of eggs of Angiostrongylus costaricensis in vitro.
abstract: Effects of the anthelmintics, pyrantel and levamisole, on egg development of Angiostrongylus costaricensis were studied in vitro. After 7 days, about 80% of eggs developed to first-stage larvae in Ham's F-12 medium with 10% foetal calf serum under 5% CO2. Significant inhibition of development was caused by pyrantel (10(-9) - 10(-8) g ml(-1)) and levamisole (10(-9) - 10(-8) g ml(-1)) (Mann-Whitney U-test; ), and none of the eggs developed to first-stage larvae in higher concentrations of these anthelmintics (10(-7) g ml(-1)). Furthermore, incubation with these drugs at 10(-8) g ml(-1) for at least 3 h or at 10(-4) g ml(-1) for 1 h caused irreversible effects on egg development.
authors.affiliation: Department of Parasitology, Hamamatsu University School of Medicine, Japan. aishih@hama-med.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Ishih
authors.forename: M
authors.initials: M
authors.lastname: Yanoh
authors.forename: C
authors.initials: C
authors.lastname: Ikeya
authors.forename: A
authors.initials: A
authors.lastname: Ban
authors.forename: M
authors.initials: M
authors.lastname: Terada
mesh_terms: D000801:Angiostrongylus / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007978:Levamisole / Q000494:pharmacology; D010063:Ovum / Q000187:drug effects* / Q000254:growth & development; D011715:Pyrantel / Q000494:pharmacology
chemical_list: D000969:Antinematodal Agents; D003470:Culture Media; D007978:Levamisole; D011715:Pyrantel