Cancer chemopreventive activity of Achyranthes aspera leaves on Epstein-Barr virus activation and two-stage mouse skin carcinogenesis.
Achyranthes aspera leaves have been assessed for chemopreventive activity. The MeOH extract, alkaloid, non-alkaloid and saponin fractions exhibited significant inhibitory effects (concentration 100 microg) on the Epstein-Barr virus early antigen activation induced by the tumor promotor 12-O-tetradecanoylphorbol-13-acetate in Raji cells. In this in vitro assay the non-alkaloid fraction containing mainly non-polar compounds showed the most significant inhibitory activity (96.9%; 60% viability). In the in vivo two-stage mouse skin carcinogenesis test the total methanolic extract possessed a pronounced anticarcinogenic effect (76%). The present study suggests that A. aspera leaf extract and the non-alkaloid fraction are valuable antitumor promotors in carcinogenesis.
Chakraborty
Asima
A
Institute of Pharmacognosy, University of Graz, Universitaetsplatz 4/I, A-8010 Graz, Austria.
Brantner
Adelheid
A
Mukainaka
Teruo
T
Nobukuni
Yoshitoshi
Y
Kuchide
Masashi
M
Konoshima
Takao
T
Tokuda
Harukuni
H
Nishino
Hoyoku
H
D029044:Achyranthes / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D004854:Herpesvirus 4, Human / Q000254:growth & development*; D051379:Mice; D008813:Mice, Inbred ICR; D010212:Papilloma / Q000139:chemically induced / Q000517:prevention & control* / Q000821:virology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism; D028321:Plant Preparations / Q000494:pharmacology; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control* / Q000821:virology; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014407:Tumor Cells, Cultured; D014775:Virus Activation / Q000187:drug effects
D000970:Antineoplastic Agents; D010936:Plant Extracts; D028321:Plant Preparations; D013755:Tetradecanoylphorbol Acetate