title: Summating potential in the guinea pig cochlea after perilymphatic perfusion with arginine-vasopressin.
abstract: We hypothesized that arginine-vasopressin (AVP), the natural vasopressin in the guinea pig, might increase the cochlear summating potential, indicating an increase in endolymphatic volume. Guinea pig cochleas were perfused with artificial perilymph for 15 min, with or without AVP (2 x 10(-6) M). In 1 group of animals, summating potentials (SP), compound action potentials and cochlear microphonics evoked by 2, 4 and 8 kHz tone bursts were measured with an apically placed electrode 15 min, 1 and 2 h after perilymphatic perfusion. In another group of animals the SP and endocochlear potential (EP) were measured simultaneously in the scala media during and after perfusion. In both groups the SP had increased significantly 15 min after perfusion with AVP and this increase was reversible. At the concentration of AVP used the increase in SP was not related to EP alterations. On light microscopic examination of the cochlea no evident increase in scala media volume could be detected. The increase in the SP (a sensitive indicator of acute endolymphatic hydrops) after perfusion with AVP suggests that this neuropeptide plays a role in the regulation of the ion and fluid balance in the cochlea.
authors.affiliation: Department of Otorhinolaryngology/Head & Neck Surgery, University Medical Center, Utrecht, The Netherlands. P.Lohuis@kmb.azu.nl
authors.forename: P J
authors.initials: PJ
authors.lastname: Lohuis
authors.forename: S F
authors.initials: SF
authors.lastname: Klis
authors.forename: M G
authors.initials: MG
authors.lastname: van Emst
authors.forename: G F
authors.initials: GF
authors.lastname: Smoorenburg
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001127:Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology*; D003051:Cochlea / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D018159:Endolymphatic Hydrops / Q000175:diagnosis / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D008853:Microscopy; D010498:Perilymph / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D012533:Scala Tympani / Q000378:metabolism / Q000473:pathology; D014662:Vasoconstrictor Agents / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D015221:Potassium Channels; D014662:Vasoconstrictor Agents; D001127:Arginine Vasopressin