[Methodology of DNA fingerprinting and its application in identification of M. tuberculosis].
OBJECTIVE
To explore methodology of DNA fingerprinting technique and its application in identification of strains of M. tuberculosis.


METHODS
This research was based on different copy numbers and location of chromosome DNA IS6110 in M. tuberculosis genome. After DNA of M. tuberculosis were cut by endonuclease PvuII, the products were transferred to nylon membrane, then detected and hybridized by enhanced chemiluminescent (ECL) labeling techniques.


RESULTS
Fifty clinical isolates from different TB patients had unidentical DNA fingerprinting patterns. DNA fingerprinting patterns showed a great difference from sputum and pus specimens of the same patient. H(37) R(v) ofloxacin resistant and sensitive strains had an identical DNA fingerprinting pattern.


CONCLUSIONS
Identification of M. tuberculosis strains is feasible by DNA fingerprinting technique.
Li
C
C
Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149.
Ma
Y
Y
Xing
A
A
Gu
S
S
Jia
H
H
Chen
X
X
Wei
P
P
D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial