DNA microsatellite analysis for parentage control in Austrian pigs.
We present an efficient parentage control for pigs based on ten polymorphic microsatellite markers analyzed in a single PCR reaction. Assuming one known parent ("paternity control"), combined exclusion probabilities (CEPs) ranged from 99.18% (Landrace), 99.74% (Piétrain) to 99.76% (Large White) for the most important Austrian breeds. Assuming a known parent-pair ("parentage control", e.g. a substituted offspring), the CEP of the 10-plex PCR increased to 99.97% (Landrace) and 99.99% (Piétrain and Large White). We developed an additional standby battery of 5 markers, which might be applied in those cases, where the CEP of the 10-plex PCR is not sufficient. Therefore an automated, cost and time reduced genotype analysis for pigs is available.
Nechtelberger
D
D
Institute for Animal Breeding and Genetics, Vienna University of Veterinary Medicine, Austria.
Kaltwasser
C
C
Stur
I
I
Meyer
J N
JN
Brem
G
G
Mueller
M
M
Mueller
S
S
D000822:Animal Husbandry; D000818:Animals; D001331:Automation; D005260:Female; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011336:Probability; D013552:Swine / Q000235:genetics*