La autoantigen enhances translation of BiP mRNA.
Translational initiation of the human BiP mRNA is directed by an internal ribosomal entry site (IRES) located in the 5'-untranslated region (5'-UTR). In order to understand the mechanism of the IRES-dependent translation of BiP mRNA, cellular proteins interacting with the BiP IRES were investigated. La autoantigen, which augments the translation of polioviral mRNA and hepatitis C viral mRNA, bound specifically to the second half of the 5'-UTR of the BiP IRES and enhanced translation of BiP mRNA in both in vitro and in vivo assays. This finding suggests that cellular and viral IRESs containing very different RNA sequences may share a common mechanism of translation.
Kim
Y K
YK
Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang, Kyungbuk 790-784, Korea.
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S H
SH
Rho
J
J
Lee
S H
SH
Jang
S K
SK
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006360:Heat-Shock Proteins; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics*; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D000097585:SS-B Antigen
D020121:5' Untranslated Regions; D001324:Autoantigens; D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D049452:Green Fluorescent Proteins; D006360:Heat-Shock Proteins; D008156:Luciferases; D008164:Luminescent Proteins; D018832:Molecular Chaperones; D012313:RNA; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D000097585:SS-B Antigen