NADPH-dependent L-sorbose reductase is responsible for L-sorbose assimilation in Gluconobacter suboxydans IFO 3291.
The NADPH-dependent L-sorbose reductase (SR) of L-sorbose-producing Gluconobacter suboxydans IFO 3291 contributes to intracellular L-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced L-sorbose, indicating that the SR functions mainly as an L-sorbose-reducing enzyme in vivo and not as a D-sorbitol-oxidizing enzyme.
Shinjoh
Masako
M
Department of Applied Microbiology, Nippon Roche Research Center, 200 Kajiwara, Kamakura 247, Japan. masako.shinjoh@roche.com
Tazoe
Masaaki
M
Hoshino
Tatsuo
T
D002237:Carbohydrate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D020576:Gluconobacter / Q000201:enzymology*; D008954:Models, Biological; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D013012:Sorbitol / Q000378:metabolism; D013013:Sorbose / Q000378:metabolism*
D013012:Sorbitol; D002237:Carbohydrate Dehydrogenases; C446052:NADPH-dependent L-sorbose dehydrogenase; D013013:Sorbose