Coexistence of antitopoisomerase I and anticentromere antibodies in patients with systemic sclerosis.
BACKGROUND
Antibodies targeting DNA topoisomerase I (ATA) or centromere proteins (ACA) are associated with clinical subsets of patients with systemic sclerosis (SSc). The occurrence of those autoantibodies is considered to be mutually exclusive.


OBJECTIVE
To describe the clinical and immunogenetic data of three patients who are co-expressing both antibodies, and then review previous publications.


METHODS
Both antibodies were detected by different methods, including indirect immunofluorescence technique, enzyme linked immunosorbent assay, immunodiffusion, and immunoblot. Patients were HLA typed by serological and molecular genetic methods. Data were extracted from published reports for comparison. The search for published studies was through Medline and other database research programmes.


RESULTS
During routine laboratory diagnostics over several years three patients with scleroderma and coincidence of ATA and ACA were identified: patient 1 with diffuse SSc, Raynaud's phenomenon, puffy fingers and fingertip necrosis, contractures, and calcinosis; patient 2 with diffuse SSc, Raynaud's phenomenon, oedema of the hands, and interstitial calcinosis of hands, knees, and shoulders, and pulmonary fibrosis; patient 3 with scleroderma of hands, forearms, and face, Raynaud's phenomenon, puffy fingers, finger contractures, fingertip necrosis, and calcinosis. All three patients studied were carriers of HLA alleles known to be associated with these autoantibodies. In serial measurements the concentrations of the two antibodies showed independent or even reverse fluctuations. Screening of 100 patients with ACA for ATA and vice versa disclosed no further patients with coincidence of these antibodies. Twenty eight cases of ACA/ATA coexistence in 5423 patients (0.52%) with SSc or SSc associated symptoms were found in an analysis of published studies.


CONCLUSIONS
The expression of ATA and ACA is not totally mutually exclusive, but coincidence is rare (<1% of patients with SSc). Patients with both autoantibodies often have diffuse scleroderma and show immunogenetic features of both antibody defined subsets of SSc.
Dick
T
T
Rheumaklinik u Rheumaforschungsinstitut, Aachen, Germany. mail@thomas-dick.de
Mierau
R
R
Bartz-Bazzanella
P
P
Alavi
M
M
Stoyanova-Scholz
M
M
Kindler
J
J
Genth
E
E
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D002503:Centromere / Q000276:immunology*; D004264:DNA Topoisomerases, Type I / Q000276:immunology*; D006801:Humans; D007159:Immunologic Tests; D008875:Middle Aged; D012595:Scleroderma, Systemic / Q000276:immunology*
D001323:Autoantibodies; D004264:DNA Topoisomerases, Type I