title: [Expression of transforming growth factor-beta 1 mRNA during mouse molar morphogenesis].
abstract: OBJECTIVE
  To investigate the temporal and spatial expression and localization of transforming growth factor-beta 1 mRNA during mouse molar morphogenesis.
  
  
  METHODS
  The distribution pattern of TGF-beta 1 mRNA was analysed on 5 microns serial sections of paraformaldehyde-fixed and paraffin-embedded embryonic mouse heads or mandibles using in situ hybridization.
  
  
  RESULTS
  The results show that TGF-beta 1 mRNA was expressed during mouse molar morphogenesis in a temporally and spatially regulated fashion. Local expression of TGF-beta 1 mRNA in the dental epithelium at bud-staged (E13) and cap-staged (E15) teeth were observed. During bell stage (E16-18), TGF-beta 1 mRNA was very abundant in the ameloblast layer and dental papilla cells. The expression of TGF-beta 1 was increased in the layer of odontoblast and ameloblast with the differentiation of these cells.
  
  
  CONCLUSIONS
  These results suggest that TGF-beta 1 may have an important role in tooth morphogenesis, and TGF-beta 1 acts as a paracrine and autoinducing factor.
authors.affiliation: Department of Endodontics, College of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: M
authors.initials: M
authors.lastname: Fan
authors.forename: Z
authors.initials: Z
authors.lastname: Bian
mesh_terms: D000818:Animals; D051379:Mice; D008963:Molar / Q000196:embryology* / Q000378:metabolism; D009024:Morphogenesis; D012333:RNA, Messenger / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta