[Polymorphism of human platelets alloantigens in Chinese patients with acute myocardial infarction and acute ischemic stroke].
OBJECTIVE
To investigate the gene frequencies of 5 major human platelets alloantigens (HPA 1 - 5) in Chinese population and to assess if polymorphism of HPA is associated with acute myocardial infarction (AMI) and acute ischemic stroke (AIS) in the Chinese.


METHODS
HPA 1 - 5 genotyping was performed with PCR using allele specific primers and restriction enzyme digestion based on PCR products in 95 AMI patients, 188 AIS patients and 270 normal controls. Gene frequency distribution was tested by Hardy-Weinberg equilibrium. Comparisons of HPA gene frequencies between the patient and control groups were made by chi(2) test.


RESULTS
The gene frequencies of HPA 1 - 5 were listed as follows: HPA1a, 91%; HPA 1b, 9%; HPA 2a, 94%; HPA 2b, 6%; HPA 3a, 83%; HPA 3b, 17%; HPA 4a, 98%; HPA 4b, 2%; HPA 5a, 97%; HPA 5b, 3%. No significant differences were found in HPA 1 - 5 gene frequencies between AMI patients and normal controls. In AIS patient group, HPA-2a allele frequency was significantly higher than that in controls, but this allele gene frequency in the two groups was very close (0.99 and 0.94, respectively) and too many subjects in the two groups were overlapped. No differences were found in the other 4 HPA systems between patients and controls.


CONCLUSIONS
Polymorphism of HPA is not an inherited risk factor and is not associated with Chinese arterial thrombotic diseases, such as AMI and AIS.
Chen
F
F
Department of Hematology, Xiang Ya Hospital, Hunan Medical University, Changsha 410008, China.
Jian
Z
Z
Xie
Q
Q
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016824:Antigens, Human Platelet / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D002545:Brain Ischemia / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D020521:Stroke / Q000235:genetics*
D016824:Antigens, Human Platelet