Sterol and fatty acid composition of Candida lusitaniae clinical isolates.
The sterol and fatty acid compositions of four amphotericin B-resistant and of two amphotericin B-susceptible Candida lusitaniae clinical isolates were determined. A flow cytofluorometric susceptibility test (FCST) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. Compared to susceptible isolates, resistant ones showed a greatly reduced ergosterol content and changes in sterol composition, consistent with a defect in Delta8-->7 isomerase. Within each group, no correlation between the sterol or fatty acid pattern or composition and both the degree of in vitro susceptibility and FCST MIC was found.
Peyron
F
F
Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire 13385 Marseille, France.
Favel
A
A
Calaf
R
R
Michel-Nguyen
A
A
Bonaly
R
R
Coulon
J
J
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry*; D006801:Humans; D013261:Sterols / Q000737:chemistry*
D000935:Antifungal Agents; D005227:Fatty Acids; D013261:Sterols; D000666:Amphotericin B