title: The use of hirudin as universal anticoagulant in haematology, clinical chemistry and blood grouping.
abstract: Undesirable interactions between anticoagulants and diagnostic test kit procedures so far have prevented the development of a single uniform blood sampling tube. Contrary to K2-EDTA, heparin and other anticoagulants, hirudin only minimally alters blood cells and dissolved blood constituents, thus qualifying as a universal anticoagulant for diagnostic purposes. Automated complete blood counts, automated analyses of clinical chemistry analytes and immunohaematology were performed from hirudinised and routinely processed blood obtained from healthy volunteers (n=35) and hospitalised patients (n=45). Hirudin (400 ATU/ml blood) sufficiently anticoagulated blood for diagnostic purposes. The measurements of automated complete blood counts obtained from K2-EDTA-anticoagulated and hirudinised blood correlated significantly as did the measurements of 24 clinical chemistry analytes from hirudinised plasma and serum. Regression analysis revealed that the results of complete blood counts and clinical chemistry tests were predictable from the respective measurements from hirudinised blood (p=0.001). Immunohaematological tests and cross-matching from hirudinised and native blood of the same donors gave identical results. Single clotting factors, but not global coagulation analytes, could be measured from hirudinised blood. Therefore, a universal hirudin-containing blood sampling tube could be designed for automated analysis of haematological, serological and clinical chemistry analytes.
authors.affiliation: Department of Internal Medicine III Haematology, Oncology and Transfusion Medicine, Universitätsklinikum Benjamin Franklin, Berlin, Germany. hmenssen@zedat.fu-berlin.de
authors.forename: H D
authors.initials: HD
authors.lastname: Menssen
authors.forename: K
authors.initials: K
authors.lastname: Melber
authors.forename: N
authors.initials: N
authors.lastname: Brandt
authors.forename: E
authors.initials: E
authors.lastname: Thiel
mesh_terms: D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D001772:Blood Cell Count / Q000379:methods; D001774:Blood Chemical Analysis / Q000379:methods; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests / Q000379:methods; D001800:Blood Specimen Collection / Q000379:methods; D004492:Edetic Acid / Q000494:pharmacology; D006403:Hematologic Tests / Q000379:methods*; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D015203:Reproducibility of Results; D012698:Serologic Tests / Q000379:methods
chemical_list: D000925:Anticoagulants; D006629:Hirudins; D004492:Edetic Acid