Virus-cell fusion inhibitory activity for the polysaccharides from various Korean edible clams.
In order to find potent virus-cell fusion inhibitory components from Korean edible clams, thirteen prepared polysaccharides were introduced to syncytia formation inhibition assay, which is based on the interaction between the HIV-1 envelope protein gp120/41 and the cellular membrane protein CD4 of T lymphocytes. Among them, Meretrix petechialis showed a potent virus-cell fusion inhibitory activity. Fusion index (FI) and percent (%) fusion inhibition of the polysaccharide of this clam were 0.21 +/- 0.02, and 67.52 +/- 4.09 at 100 microg/ml, respectively. It exhibited almost equivalent virus-cell fusion inhibitory activity to that of dextran sulfate which was used as a standard control.
Woo
E R
ER
College of Pharmacy, Chosun University, Kwangju, Korea. wooer@mail.chosun.ac.kr
Kim
W S
WS
Kim
Y S
YS
D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D049872:Bivalvia / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008561:Membrane Fusion / Q000187:drug effects*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D019380:Anti-HIV Agents; D011134:Polysaccharides