Sensitization of a tumor, but not normal tissue, to the cytotoxic effect of ionizing radiation using Panax notoginseng extract.
The aim of the present study was to investigate any sensitization effect of the Panax notoginseng extract (PNE) and the purified Saponin (Rb1) on the radiation response of an experimental tumor (KHT sarcoma) in mice, in comparison with any effects on a normal tissue (bone marrow). PNE at a concentration of 0.1-100 mg/kg produced an increase in tumor radiosensitivity. The sensitization effect was maximal at 10 mg/kg and at 30 minutes after injection. Higher doses were toxic to the bone marrow stem cells. Similarly Rb1 at a concentration 0.001 to 1 mg/kg also produced an increase in tumor radiosensitivity, with maximum effect at 1 mg/kg. Higher doses were not toxic to the bone marrow stem cells in this case. Radiosensitization factors were calculated as ratios of D0 (the radiosensitivity parameter), and these were highly significant for the tumor and very similar for both compounds at the doses used, namely 1.18-1.19. There was no significant effect for bone marrow stem cells (sensitization factors of 0.99 +/- 0.01 for both compounds). The differential effect on tumor, and the magnitude of the radiosensitization, suggest that further purified or synthetic versions of this extract may be useful not only in vascular-related diseases but also in cancer therapy.
Chen
F D
FD
Faculty of Medical Radiation Technology and Institute of Radiological Sciences, National Yang Ming University, Taiwan.
Wu
M C
MC
Wang
H E
HE
Hwang
J J
JJ
Hong
C Y
CY
Huang
Y T
YT
Yen
S H
SH
Ou
Y H
YH
D000818:Animals; D003131:Combined Modality Therapy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use* / Q000633:toxicity; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D005894:Panax / Q000633:toxicity; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D012503:Saponins / Q000627:therapeutic use / Q000633:toxicity; D012513:Sarcoma, Experimental / Q000188:drug therapy* / Q000532:radiotherapy*
D004365:Drugs, Chinese Herbal; C079995:Panax quinquefolium saponin; D012503:Saponins