Gentamicin enzyme-linked immunosorbent assay for microdialysis samples.
The authors investigated the use of a commercially available gentamicin enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of gentamicin concentrations in microdialysis samples. The assay demonstrated good accuracy and precision in the concentration range of 100 to 967 pg/mL. The developed quantitative ELISA is a highly sensitive and valid method for measuring gentamicin concentrations in microdialysis samples. This assay may be a useful alternative to other assay methodologies where analysis may be restricted by sample volume requirements and limited sensitivity.
Hanes
S D
SD
Department of Clinical Pharmacy, University of Tennessee, College of Pharmacy, Memphis, TN 38163, USA. shanes@utmem.edu.
Herring
V L
VL
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005839:Gentamicins / Q000032:analysis*; D017551:Microdialysis; D013552:Swine
D000900:Anti-Bacterial Agents; D005839:Gentamicins