A chemiluminescence reaction between hydrogen peroxide and acetonitrile and its applications.
Here we report a new chemiluminescence reaction between basic aqueous H2O2 and acetonitrile. Its ultraweak chemiluminescence could be greatly enhanced by luminol, isoluminol-labeled streptavidin, and an Edman-type fluorescent reagent. Light emission was intense and long-lived, and this facilitated the initiation of the reaction and the measurement of the light emission. The present results permit us to propose a series of convenient, highly sensitive, and enzyme-free techniques for the detection and quantification of luminol, related conjugates, acetonitrile, and amino acids. Overall, this new chemiluminescence reaction will be quite promising for numerous applications in immunoassay, DNA hybridization, environmental monitoring. and postcolumn chromatographic detection.
Lu
J
J
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Lau
C
C
Morizono
M
M
Ohta
K
K
Kai
M
M
D000097:Acetonitriles / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D011506:Proteins / Q000032:analysis; D012997:Solvents / Q000032:analysis
D000097:Acetonitriles; D000596:Amino Acids; D007202:Indicators and Reagents; D011506:Proteins; D012997:Solvents; D008165:Luminol; D006861:Hydrogen Peroxide