title: Hormone sensitive lipase expression and adipose tissue metabolism show gender difference in obese subjects after weight loss.
abstract: OBJECTIVE
  The effect of weight reduction on hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) gene expression and their relationship with adipose tissue metabolism were studied in massively obese men and women.
  
  
  METHODS
  Seventeen obese subjects (eight men, nine women) participated in the study (age 44+/-2 y, weight 145+/-8 kg, fat 40+/-2% of body mass, mean+/-s.e.m.), who were going through a gastric-banding operation for weight reduction.
  
  
  METHODS
  HSL and LPL mRNA expressions were analyzed using the reverse transcription competitive polymerase chain reaction. Subcutaneous fat lipolysis was measured in vivo by microdialysis and in vitro in isolated subcutaneous abdominal adipocytes. Measurements were done before and after 1 y of weight reduction.
  
  
  RESULTS
  Significant reductions in weight (for men -20.3+/-2.5%, for women -18.3+/-2.1% (mean+/-s.e.m.) and fat mass (for men -27.6+/-7.9%, for women -21.8+/-3.9%) were observed in both genders. In women HSL mRNA expression decreased by 31% (P=0.008) and LPL expression increased slightly, but nonsignificantly (42%, P=0.110). These changes were not observed in men. In men, inhibition of lipolysis with alpha(2)-adrenergic and adenosine agonist was improved (P=0.001) in isolated adipocytes.
  
  
  CONCLUSIONS
  This study uncovers new differences between genders in adipocyte metabolism along with weight reduction. In women, the observed changes in HSL and LPL gene expression suggest that deposition of lipids into adipose tissue might be favored after weight reduction. In men, the results indicate improved responsiveness to inhibition in adipose tissue metabolism along with weight reduction.
authors.affiliation: Department of Clinical Nutrition, University of Kuopio and Kuopio University Hospital, Kuopio, Finland. Marjukka.Kolehmainen@uku.fi
authors.forename: M
authors.initials: M
authors.lastname: Kolehmainen
authors.forename: H
authors.initials: H
authors.lastname: Vidal
authors.forename: J J
authors.initials: JJ
authors.lastname: Ohisalo
authors.forename: E
authors.initials: E
authors.lastname: Pirinen
authors.forename: E
authors.initials: E
authors.lastname: Alhava
authors.forename: M I J
authors.initials: MI
authors.lastname: Uusitupa
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008066:Lipolysis / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012723:Sex; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism*; D015431:Weight Loss
chemical_list: D012333:RNA, Messenger; D002787:Sterol Esterase; D008071:Lipoprotein Lipase