title: Quantitative expression of adhesion molecules on granulocyte colony-stimulating factor-mobilized peripheral blood, bone marrow, and cord blood CD34+ cells.
abstract: The purpose of this study is to investigate the function of the main adhesion receptors on CD34(+) cells during hematopoietic stem cell transplantation. Expression was quantified by flow cytometry using calibration beads. CD34(+) cells were isolated from either bone marrow (BM), cord blood (CB), or peripheral blood (PB) from study patients and a control group after granulocyte colony-stimulating factor (G-CSF) administration. The study of the CD34(+) cell differentiation showed that CD34(+) cells are mainly CD38(+) and HLA-DR(+), whatever the type of harvest. However, quantitative analysis elicited a weaker expression of CD38 on PB and CB CD34(+) cells in comparison to BM CD34(+) cells. The proportions of CD34(+)/CD49d(+) and CD34(+)/CD49e(+) were smaller on PB cells, without quantitative expression variation. This phenotypic variation promotes CD34(+) cells to exit from BM into circulation, inducing the mobilization. The homing of the CD34(+) cells to the BM involves the CD62L receptor. The expression of this receptor was found to be more frequent and stronger on PB cells than on BM or CB cells. The CD11b, CD18, and CD54 receptors are implicated in CD34(+) cell adhesion to BM microenvironment. No significant variation in CD34(+)/CD11b(+) and CD34(+)/CD18(+) cell frequency was noted. Moreover, the CD54 receptor was more frequently expressed on CB and PB cells. Quantitative analysis revealed that CD18 was more strongly expressed on BM than on PB cells to promote progenitors adhesion by interacting with stromal cells. Finally, the quantitative expression of the main receptors on CD34(+) cells explained cellular functions during the different steps of hematopoietic stem cells transplantation.
authors.affiliation: Mécanique et Ingenierie Cellulaire et Tissulaire, LEMTA UMR CNRS 7563, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France.
authors.forename: C
authors.initials: C
authors.lastname: Gigant
authors.forename: V
authors.initials: V
authors.lastname: Latger-Cannard
authors.forename: D
authors.initials: D
authors.lastname: Bensoussan
authors.forename: P
authors.initials: P
authors.lastname: Feugier
authors.forename: P
authors.initials: P
authors.lastname: Bordigoni
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000032:analysis; D051997:ADP-ribosyl Cyclase 1; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D001773:Blood Cells / Q000166:cytology / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002452:Cell Count; D002648:Child; D016238:Cytapheresis; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000032:analysis; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D039041:Integrin alpha4beta1 / Q000032:analysis; D039081:Integrin alpha5beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D019041:L-Selectin / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000032:analysis; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009101:Multiple Myeloma / Q000473:pathology / Q000628:therapy; D011956:Receptors, Cell Surface / Q000032:analysis*; D033581:Stem Cell Transplantation
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1