PDGF receptor-alpha deficiency in glomerular mesangial cells of tenascin-C knockout mice.
Tenascin-C (TNC) knockout (TNKO) mice showed reduced proliferation of mesangial cells and abnormal restoration after habu-snake venom (HSV)-induced glomerulonephritis. In this study, we examined the relationship of TNC and platelet-derived growth factor receptor (PDGFR) in glomerular mesangial cells. TNC and PDGFR-alpha and -beta transcriptions were up-regulated in wild type (WT) mice after HSV injection, but in TNKO mice PDGFR-alpha transcription was not up-regulated. Immunohistochemistry showed that PDGFR-alpha was found in mesangial areas of colocalized alpha-smooth muscle actin, but in TNKO mice it was not detectable. In vitro studies showed that the expressions of PDGFR-alpha and -beta mRNA and protein in cultured glomerular mesangial cells (GMC) of TNKO mice were lower than those in WT GMC. These results suggest that failures of both TNC and PDGFR-alpha are a candidate for abnormal restoration of TNKO mice.
Matsumoto
Kenji
K
Experimental Animal Division, Bio Resource Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan. kenjimat@rtc.riken.go.jp
Hiraiwa
Noriko
N
Yoshiki
Atsushi
A
Ohnishi
Masatake
M
Kusakabe
Moriaki
M
D000818:Animals; D002455:Cell Division; D003435:Crotalid Venoms / Q000633:toxicity; D005920:Glomerular Mesangium / Q000378:metabolism* / Q000473:pathology; D005921:Glomerulonephritis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019063:Tenascin / Q000172:deficiency* / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D017841:Trimeresurus
D003435:Crotalid Venoms; D012333:RNA, Messenger; D019063:Tenascin; D020797:Receptor, Platelet-Derived Growth Factor beta