title: Rapid chromosome detection by PRINS in human sperm.
abstract: Primed in situ (PRINS) labeling may be used for direct estimation of disomy rate in human sperm. We combined the PRINS procedure with a rapid and efficient NaOH pretreatment allowing simultaneous decondensation and denaturation of sperm nuclei. This enabled double labeling of human sperm within a 2-hr time span. Chromosome-specific primers have been defined for most human chromosomes, including those most frequently involved in aneuploidy. Using the dual-color PRINS method, we estimated incidences of disomy for 15 autosomes and the sex chromosomes in sperm samples from 6 normal fertile men. The frequency of disomy ranged from 0.28% to 0.36%. There were no significant interchromosomal differences in disomy rate, but chromosome 21 displayed a higher incidence of nondisjunction than did the other chromosomes.
authors.affiliation: CNRS UPR 1142, Institute of Human Genetics, Montpellier, France. Frank.Pellestor@igh.cnrs.fr
authors.forename: Franck
authors.initials: F
authors.lastname: Pellestor
authors.forename: Isabelle
authors.initials: I
authors.lastname: Imbert
authors.forename: Brigitte
authors.initials: B
authors.lastname: Andréo
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004171:Diploidy; D006801:Humans; D008297:Male; D020401:Primed In Situ Labeling / Q000379:methods*; D012730:Sex Chromosomes; D013094:Spermatozoa / Q000648:ultrastructure*
chemical_list: D017931:DNA Primers