title: Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency.
abstract: Because of its stringent sequence specificity, the catalytic domain of the nuclear inclusion protease from tobacco etch virus (TEV) is a useful reagent for cleaving genetically engineered fusion proteins. However, a serious drawback of TEV protease is that it readily cleaves itself at a specific site to generate a truncated enzyme with greatly diminished activity. The rate of autoinactivation is proportional to the concentration of TEV protease, implying a bimolecular reaction mechanism. Yet, a catalytically active protease was unable to convert a catalytically inactive protease into the truncated form. Adding increasing concentrations of the catalytically inactive protease to a fixed amount of the wild-type enzyme accelerated its rate of autoinactivation. Taken together, these results suggest that autoinactivation of TEV protease may be an intramolecular reaction that is facilitated by an allosteric interaction between protease molecules. In an effort to create a more stable protease, we made amino acid substitutions in the P2 and P1' positions of the internal cleavage site and assessed their impact on the enzyme's stability and catalytic activity. One of the P1' mutants, S219V, was not only far more stable than the wild-type protease (approximately 100-fold), but also a more efficient catalyst.
authors.affiliation: Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, PO Box B, Frederick, MD 21702-1201, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Kapust
authors.forename: J
authors.initials: J
authors.lastname: Tözsér
authors.forename: J D
authors.initials: JD
authors.lastname: Fox
authors.forename: D E
authors.initials: DE
authors.lastname: Anderson
authors.forename: S
authors.initials: S
authors.lastname: Cherry
authors.forename: T D
authors.initials: TD
authors.lastname: Copeland
authors.forename: D S
authors.initials: DS
authors.lastname: Waugh
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D020134:Catalytic Domain; D010450:Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D004795:Enzyme Stability; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D015202:Protein Engineering; D016415:Sequence Alignment
chemical_list: D010450:Endopeptidases; C099488:TEV protease