title: Role of DNA polymerase II in the tolerance of thymine dimers remaining unexcised in UV-irradiated Escherichia coli exposed to pre-UV nutritional stress.
abstract: Nutritional stress applied prior to UV-irradiation to E. coli 15 555-7 reduced thymine dimer excision and inhibited post-UV incorporation of thymidine in polB(+) as well as in polB(-) cells. However, the pre-UV-stressed polB(+) cells were significantly more UV-resistant and after UV synthesized larger DNA molecules than the pre-UV-stressed polB(-) cells. The data suggest that DNA polymerase II is involved in the tolerance of unremoved thymine dimers.
authors.affiliation: Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Sedliakova
authors.forename: V
authors.initials: V
authors.lastname: Slezarikova
authors.forename: F
authors.initials: F
authors.lastname: Masek
authors.forename: M
authors.initials: M
authors.lastname: Vizvaryova
authors.forename: M
authors.initials: M
authors.lastname: Pirsel
mesh_terms: D003470:Culture Media; D004249:DNA Damage; D004257:DNA Polymerase II / Q000502:physiology*; D004269:DNA, Bacterial / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D011740:Pyrimidine Dimers / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D011740:Pyrimidine Dimers; C074431:polB protein, E coli; D004257:DNA Polymerase II