title: Differential expression of cytokines in rat osteosarcoma and malignant fibrous histiocytoma cell lines induced by 4-(hydroxyamino)quinoline-1-oxide.
abstract: Cytokines are considered to play an important role in tumor pathogenesis and progression, and recent studies have demonstrated that a variety of forms, including interleukins (ILs) and transforming growth factor-beta(s) (TGF-beta(s)), may regulate tumors. In the present study, the expression of TGF-beta isoforms and ILs was investigated in cell lines from a rat osteosarcoma and a malignant fibrous histiocytoma (MFH), both established from transplantable tumors induced by 4-(hydroxyamino) quinoline 1-oxide (4-HAQO) in syngeneic F344 male rats. The results of a multiprobe RNase protection assay showed TGF-beta1 expression to be remarkably elevated, with no TGF-beta2 and beta3 detectable in MFH cells, while TGF-beta1 and -beta2 were found to be moderately and TGF-beta3 weakly expressed in osteosarcoma lines. All cell lines of osteosarcomas and MFHs expressed macrophage migration inhibitory factor at similar levels. In contrast to the lack of ILs in the MFH cells, moderate IL-6 and very weak IL-1beta expression was detected in the osteosarcoma cells. These results suggest that variation in expression pattern of these cytokines in osteosarcomas and MFHs might be involved in differences in histological appearance and biological behavior, including metastatic ability, between these two mesenchyme-derived tumor types.
authors.affiliation: Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan.
authors.forename: Kanya
authors.initials: K
authors.lastname: Honoki
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tsujiuchi
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Toru
authors.initials: T
authors.lastname: Morishita
authors.forename: Akira
authors.initials: A
authors.lastname: Kido
authors.forename: Yoshizumi
authors.initials: Y
authors.lastname: Miyauchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Mii
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Takakura
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Konishi
mesh_terms: D015108:4-Hydroxyaminoquinoline-1-oxide / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects; D018219:Histiocytoma, Benign Fibrous / Q000378:metabolism* / Q000473:pathology; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D007378:Interleukins; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D015108:4-Hydroxyaminoquinoline-1-oxide