Sequence organization of barley centromeres.
By sequencing, fingerprinting and in situ hybridization of a centromere-specific large insert clone (BAC 7), the sequence organization of centromeric DNA of barley could be elucidated. Within 23 kb, three copies of the Ty3/gypsy-like retroelement cereba were present. Two elements of approximately 7 kb, arranged in tandem, include long terminal repeats (LTRs) (approximately 1 kb) similar to the rice centromeric retrotransposon RIRE 7 and to the cereal centromeric sequence family, the primer binding site, the complete polygene flanked by untranslated regions, as well as a polypurine tract 5' of the downstream LTR. The high density (approximately 200 elements/centromere) and completeness of cereba elements and the absence of internally deleted elements and solo LTRs from the BAC 7 insert represent unique features of the barley centromeres as compared to those of other cereals. Obviously, the conserved cereba elements together with barley-specific G+C-rich satellite sequences constitute the major components of centromeric DNA in this species.
Hudakova
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Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany.
Michalek
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W
Presting
G G
GG
ten Hoopen
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R
dos Santos
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K
Jasencakova
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Z
Schubert
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I
D015139:Blotting, Southern; D002503:Centromere / Q000235:genetics*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001467:Hordeum / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D018626:Retroelements; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D018626:Retroelements; D004262:DNA Restriction Enzymes