Prostaglandin biosynthesis by fat body from larvae of the beetle Zophobas atratus.
We describe prostaglandin (PG) biosynthesis by microsomal-enriched fractions of fat body prepared from larvae of the tenebrionid beetle, Zophobas atratus. PG biosynthesis was sensitive to incubation time, temperature, pH, substrate and protein concentration. Optimal PG biosynthesis conditions of those we examined included 2 mg of microsomal-enriched protein, incubated at 22 degrees C for 2 min at pH 6. These preparations yielded four major PGs: PGA(2), PGE(2), PGD(2) and PGF(2 alpha). PGA(2) and PGF(2 alpha) were the predominant eicosanoids produced under these conditions. Two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, effectively inhibited PG biosynthesis in low concentrations. In vitro PG biosynthetic reaction conditions, using vertebrate or invertebrate enzyme sources, usually include a cocktail of reaction co-factors. The Z. atratus preparation similarly performs better in the presence of co-factors. Arch.
Tunaz
Hasan
H
Insect Biochemical Physiology Laboratory, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0816, USA.
Putnam
Sean M
SM
Stanley
David W
DW
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001517:Coleoptera / Q000187:drug effects / Q000378:metabolism*; D005216:Fat Body / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D019476:Insect Proteins / Q000378:metabolism; D007553:Isotope Labeling; D007814:Larva / Q000378:metabolism; D009288:Naproxen / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis*; D013347:Subcellular Fractions; D013379:Substrate Specificity; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019476:Insect Proteins; D011453:Prostaglandins; D009288:Naproxen; D007213:Indomethacin