title: Nucleotide sequence and organization of plasmid pMVSCS1 from Mannheimia varigena: identification of a multiresistance gene cluster.
abstract: OBJECTIVE
  A small resistance plasmid of Mannheimia varigena was analysed with regard to its gene organization and the development of a multiresistance gene cluster.
  
  
  METHODS
  The 5621 bp plasmid pMVSCS1 was transformed into Escherichia coli JM107, cloned and sequenced completely.
  
  
  RESULTS
  Three intact resistance genes, sulII, catAIII and strA, a truncated strB gene and a novel replication gene were identified. A potential recombination site for the integration of catAIII in the spacer region between sulII and strA was identified.
  
  
  CONCLUSIONS
  The physical linkage of the plasmid-borne resistance genes organized in a cluster would facilitate the spread of the different resistance genes by co-selection, even in the absence of a direct selective pressure.
authors.affiliation: Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft (FAL), Dörnbergstrasse 25-27, 29223 Celle, Germany.
authors.forename: Corinna
authors.initials: C
authors.lastname: Kehrenberg
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwarz
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics*; D001996:Bronchopneumonia / Q000382:microbiology; D002701:Chloramphenicol / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D023061:Gene Order; D005798:Genes, Bacterial; D005810:Multigene Family / Q000235:genetics*; D015661:Pasteurellaceae / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D013307:Streptomycin / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D013552:Swine
chemical_list: D013449:Sulfonamides; D002701:Chloramphenicol; D013307:Streptomycin