Human non-pregnancy ribonuclease with anti-Kaposi's sarcoma activity.
Our demonstration of a 19kDa anti-Kaposi's sarcoma (KS) ribonuclease (RNase) in urine from a non-pregnant female may provide at least part of the explanation for the low incidence of KS in human females. N-terminal sequence analysis and isoelectric focusing of the purified RNase, coupled with the very low levels of anti-KS activity noted for recombinant forms of human eosinophil derived neurotoxin and human eosinophil cationic protein, suggest that the 19kDa enzyme is an eosinophilic protein whose potent anti-KS activity is dependent upon post-translational modifications that occur only in human cells.
Al-Owais
Moza M A
MM
Division of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Hesse
Lars
L
Talbot
Simon J
SJ
Adams
David J
DJ
D000230:Adenocarcinoma / Q000378:metabolism; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D047091:Eosinophil Granule Proteins; D047208:Eosinophil-Derived Neurotoxin; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics / Q000652:urine*; D012514:Sarcoma, Kaposi / Q000188:drug therapy* / Q000201:enzymology*; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D011994:Recombinant Proteins; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases