Leukemia inhibitory factor decreases the arylamine N-acetyltransferase activity in human cumulus granulosa cells.
OBJECTIVE
To evaluate the activities of acetyl coenzyme A (AcCoA):arylamine N-acetyltransferase (NAT) of intact cumulus granulosa cells and the role of leukemia inhibitory factor (LIF) upon their NAT activities.


METHODS
Thirty women accepted controlled ovarian hyperstimulation (COH) and oocyte retrievals. Human cumulus granulosa cells were obtained during oocyte retrievals. Using 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA) as substrates, NAT activity of all samples was determined by high pressure liquid chromatography. After the incubation with different time and concentrations of 2-AF, PABA, and LIF, 2-acetyl-aminofluorene (2-AAF) and N-acetyl-PABA (N-Ac-PABA) were measured.


RESULTS
After incubation with 2.812, 5.625, 11.25, and 22.5 microM of 2-AF/PABA, their product concentrations (2-AAF/N-Ac-PABA) were 0.42/0.32, 0.76/0.58, 1.29/1.04, and 1.94/1.26 nmol/10(6) cells, respectively. After 6, 12, 18, and 24 h incubation with 11.25 microM of 2-AF/PABA, their product concentrations were 0.19/0.12, 0.56/0.4, 0.98/0.79, and 1.3/1.0 nmol/10(6) cells, respectively. After incubation with 0, 5, and 50 microM of LIF, the 2-AAF/N-Ac-PABA concentrations were 0.98/0.80, 0.70/0.52, and 0.49/0.30 nmol/10(6) cells, respectively.


CONCLUSIONS
Intact human cumulus granulosa cells could acetylate arylamine carcinogen (2-AF) and noncarcinogens drug (PABA). LIF decreased the NAT activities. It provides a model for monitoring the effects of COH and LIF upon the oocytes.
Chang
C C
CC
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
Hsieh
Y Y
YY
Chung
J G
JG
Tsai
H D
HD
Tsai
C H
CH
D015073:2-Acetylaminofluorene / Q000378:metabolism; D010129:4-Aminobenzoic Acid / Q000378:metabolism / Q000494:pharmacology; D000105:Acetyl Coenzyme A / Q000378:metabolism; D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005260:Female; D005449:Fluorenes / Q000494:pharmacology; D006107:Granulosa Cells / Q000201:enzymology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology
D005449:Fluorenes; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D009153:Mutagens; C012177:2-aminofluorene; D000105:Acetyl Coenzyme A; D015073:2-Acetylaminofluorene; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid