title: Eosinophilia and cell activation mediators in nasal secretions.
abstract: OBJECTIVE
  In rhinologic disorders such as polyposis or rhinitis, nasal cytology allows differentiation between patients according to the degree of eosinophilia in nasal secretions. The egress of eosinophil and/or neutrophil polymorphonuclears from the underlying mucosa might correlate with the release of soluble mediators of cell activation such as the chemokine IL-8, and such molecules of the innate immunity as the LPS-receptor CD14 or lysozyme. We assayed the levels of these three molecules in nasal secretions in correlation with cytologic findings and especially the degree of eosinophilia.
  
  
  METHODS
  Fifty-four patients from a prospective study of nasal secretions were enrolled in this work. They constituted two groups of 27 patients each, respectively, with or without more than 20% eosinophils in nasal secretions. Nasal secretions were collected by aspiration, weighed and diluted in a fixed amount of buffer. Classic cytologic analyses were performed on the pelleted cells and IL-8, sCD14, and lysozyme levels were assayed in the cell-free supernatants.
  
  
  METHODS
  Cytologic analyses included cell-enumeration in Neubauer's chambers, and differentials performed on May-Grünwald Giemsa-stained cytospins. ELISA tests were used to assay the levels of IL-8 and sCD14. Lysozyme concentrations were assayed in immuno-nephelometry.
  
  
  RESULTS
  Significantly lower levels of IL-8 and sCD14 were observed in patients with eosinophilia than in patients with a predominance of neutrophils, whereas no difference was observed in lysozyme concentrations.
  
  
  CONCLUSIONS
  These data show that the egress of neutrophils in nasal secretions is associated with high levels of IL-8 and sCD14.
authors.affiliation: Laboratoire d'Immunologie du CHU, Faculté de Médecine, 54505, Vandoeuvre-les-Nancy Cedex, France.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Besançon-Watelet
authors.forename: Marie C
authors.initials: MC
authors.lastname: Béné
authors.forename: Paul
authors.initials: P
authors.lastname: Montagne
authors.forename: Gilbert C
authors.initials: GC
authors.lastname: Faure
authors.forename: Roger
authors.initials: R
authors.lastname: Jankowski
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004802:Eosinophilia / Q000276:immunology*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D007958:Leukocyte Count; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009113:Muramidase / Q000378:metabolism; D017817:Nasal Lavage Fluid / Q000276:immunology*; D009297:Nasal Mucosa / Q000276:immunology* / Q000378:metabolism; D009298:Nasal Polyps / Q000175:diagnosis / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D012016:Reference Values; D012220:Rhinitis / Q000175:diagnosis / Q000276:immunology*; D012852:Sinusitis / Q000175:diagnosis / Q000276:immunology*
chemical_list: D018836:Inflammation Mediators; D016209:Interleukin-8; D018950:Lipopolysaccharide Receptors; D009113:Muramidase