Involvement of trigeminal spinal nucleus in parasympathetic reflex vasodilatation in cat lower lip.
We examined whether the trigeminal spinal nucleus (Vsp) forms part of the central mechanism by which electrical stimulation of the central cut end of the lingual nerve (LN) evokes parasympathetic reflex vasodilatation in the lower lip in artificially ventilated, cervically vagosympathectomized cats deeply anesthetized with alpha-chloralose and urethane. For this purpose, we made microinjections within the brain stem to produce nonselective, reversible local anesthesia (lidocaine) or soma-selective, irreversible neurotoxic damage (kainic acid). Local anesthesia of Vsp by microinjection of lidocaine (2%; 1 microl/site) reversibly and significantly reduced the ipsilateral-LN-evoked parasympathetic reflex vasodilatation. Unilateral microinjection of kainic acid (10 mM/site; 1 microl) into Vsp ipsilateral to the stimulated LN led to an irreversible reduction in the reflex vasodilatation but had no effect on the vasodilatation elicited by stimulation of the contralateral LN. Such microinjection of kainic acid into Vsp had no effect on the vasodilatation evoked by electrical stimulation of the ipsilateral inferior salivatory nucleus. Electrical stimulation of Vsp elicited a blood flow increase in the lower lip in an intensity- and frequency-dependent manner, regardless of whether systemic arterial blood pressure rose or fell. Hexamethonium (1.0 mg/kg iv) significantly reduced the vasodilator responses elicited by electrical stimulation of the central cut end of LN or of Vsp, each to a similar degree. After hexamethonium, both vasodilator responses showed time-dependent recovery. These results strongly suggest that Vsp is an important bulbar relay for LN-evoked parasympathetic reflex vasodilatation in the cat lower lip.
Mizuta
Kentaro
K
Department of Oral Molecular Bioregulation, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Kuchiiwa
Satoshi
S
Saito
Takashi
T
Mayanagi
Hideaki
H
Karita
Keishiro
K
Izumi
Hiroshi
H
D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D002415:Cats; D004558:Electric Stimulation; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005260:Female; D005730:Ganglionic Blockers / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D007608:Kainic Acid / Q000494:pharmacology; D008012:Lidocaine / Q000494:pharmacology; D008046:Lip / Q000098:blood supply*; D008297:Male; D008845:Microinjections; D010275:Parasympathetic Nervous System / Q000502:physiology*; D012018:Reflex / Q000187:drug effects / Q000502:physiology*; D014279:Trigeminal Nucleus, Spinal / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology*
D000779:Anesthetics, Local; D018690:Excitatory Amino Acid Agonists; D005730:Ganglionic Blockers; D018738:Hexamethonium; D008012:Lidocaine; D007608:Kainic Acid