title: The effect of mesenteric lymphadenectomy and Kupffer cell depletion on bacterial translocation.
abstract: BACKGROUND
  Infectious complications are associated with high morbidity in patients with short bowel syndrome and after small bowel transplantation. Bacterial translocation from the intestine is probably an essential factor in the genesis of these infections. In a model for bacterial translocation in the rat we examined the consequence of mesenteric lymphadenectomy and the depletion of Kupffer cells.
  
  
  METHODS
  The effect of mesenteric lymphadenectomy was studied in two different models; in rats where a Thiry-Vella loop had been created from small bowel and in rats that had received a syngeneic small bowel transplant. To study the role of the Kupffer cells, rats with Thiry-Vella loops were treated intravenously with the Kupffer cell inhibitor gadolinium chloride. All animals were sacrificed on Day 3 postoperatively and the bacterial translocation to the mesenteric lymph nodes, liver, spleen, lung, and blood was evaluated.
  
  
  RESULTS
  Removal of the mesenteric lymph nodes did not result in any increased bacterial translocation in animals with a Thiry-Vella loop. However, the inactivation of Kupffer cells with gadolinium chloride produced a more severe translocation to the liver, spleen, and lungs. After small bowel transplantation the bacterial translocation to the spleen was increased in animals without mesenteric lymph nodes.
  
  
  CONCLUSIONS
  In the model of bacterial translocation from a defunctionalized loop of small bowel the inhibition of Kupffer cells will promote the systemic spread of the translocating bacteria. This indicates an important protective function of the Kupffer cells against translocating microbes.
authors.affiliation: Department of Pediatric Surgery, University Hospital, Uppsala, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Stenbäck
authors.forename: Staffan
authors.initials: S
authors.lastname: Meurling
authors.forename: Cristinel
authors.initials: C
authors.lastname: Cantar
authors.forename: Monica
authors.initials: M
authors.lastname: Lundholm
authors.forename: Johan
authors.initials: J
authors.lastname: Wallander
authors.forename: Cecilia
authors.initials: C
authors.lastname: Johnsson
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001424:Bacterial Infections / Q000401:mortality / Q000601:surgery; D018988:Bacterial Translocation / Q000502:physiology*; D005682:Gadolinium / Q000494:pharmacology; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000637:transplantation; D007728:Kupffer Cells / Q000166:cytology* / Q000187:drug effects; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000276:immunology / Q000382:microbiology / Q000601:surgery; D015996:Survival Rate
chemical_list: D000893:Anti-Inflammatory Agents; D005682:Gadolinium; C038958:gadolinium chloride