Age related effects of organochlorine insecticide lindane on intestinal brush border membrane in rats.
The effect of oral administration of lindane (gamma-HCH) has been studied on the intestine in 10-day, 20-day and 100-day old rats. In 10 day-old suckling pups exposed to lindane, there was a significant decrease in the activities of sucrase (29%), lactase (20%) and that of alkaline phosphatase (24%) compared to control. Sialic acid content of the brush borders was significantly decreased (29%) in 10-day old as well as in 20- and 100-day old rats (20 and 25% respectively), while fucose content of the membranes was significantly enhanced in all the age groups upon pesticide treatment. Among the brush border lipids, cholesterol content was significantly increased in all the age groups studied, the maximum increase of 35% being observed in 10-day-old rats. Membrane phospholipids were also increased in 20- and 100-day old animals (22% each) on lindane exposure. The present studies indicated that brush border membranes of suckling rat intestine were more susceptible to pesticide induced changes compared to older animals.
Labana
S
S
Department of Biochemistry, Panjab University, Chandigarh.
Bansal
R C
RC
Mahmood
A
A
D000284:Administration, Oral; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism; D043322:Lactase; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013393:Sucrase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D007306:Insecticides; D008563:Membrane Lipids; D001556:Hexachlorocyclohexane; D000469:Alkaline Phosphatase; D043322:Lactase; D001616:beta-Galactosidase; D013393:Sucrase; D019158:N-Acetylneuraminic Acid