title: Two "wild-type" variants of Escherichia coli sigma(70): context-dependent effects of the identity of amino acid 149.
abstract: The identity of amino acid 149 of Escherichia coli sigma(70) has been reported variably as either arginine or aspartic acid. We show that the behavior of both a region 1.2 deletion and a single-amino-acid substitution at position 122 are greatly affected by the identity of amino acid 149.
authors.affiliation: Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas 77030, USA.
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Baldwin
authors.forename: Andrea
authors.initials: A
authors.lastname: McCracken
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Dombroski
mesh_terms: D019943:Amino Acid Substitution; D000596:Amino Acids; D001216:Asparagine / Q000235:genetics*; D001224:Aspartic Acid / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D014644:Genetic Variation; D017384:Sequence Deletion; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D012808:Sigma Factor; D001224:Aspartic Acid; D001216:Asparagine; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases