[The effects of Xuezhikang on neointimal proliferation and C-myc gene expression after angioplasty in rabbits].
OBJECTIVE
To investigate the effects of Xuezhikang (XZhK) on the neointimal proliferation and C-myc gene expression after angioplasty in rabbits.


METHODS
Angioplasty for atherosclerotic stenosis of celiac arteries was performed in 30 male white rabbits after being fed with cholesterol-supplemented diet for 8 weeks, which were then randomized to control group, high-cholesterol group and XZhK group. After 4 weeks, the local vessels were collected for morphological observation. C-myc mRNA level was measured with RT-PCR and C-myc protein with immunohistochemical analysis.


RESULTS
Morphological observation showed that the neointimal area in the XZhK group were less than the control group (P < 0.05), and that in control group were less than that in the high-cholesterol group (P < 0.05). The levels of C-myc mRNA measured with RT-PCR and the percentage of C-myc protein positive cell by immunohistochemical analysis were lower in the XZhK group than in the control and high-cholesterol group (P < 0.05), The levels in the latter two groups showed no difference (P > 0.05).


CONCLUSIONS
XZhK can inhibit the neointimal proliferation and the expression of C-myc gene after angioplasty in rabbits.
Qi
G
G
Department of Cardiology, The First Affiliated Hospital of China Medical University, Shenyang 110001.
Zeng
D
D
Liu
L
L
Zhao
A
A
Xu
P
P
D000800:Angioplasty, Balloon; D000818:Animals; D002445:Celiac Artery / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002791:Cholesterol, Dietary; D004365:Drugs, Chinese Herbal; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger