A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp. strain 7.
An enzyme, which catalyzes both decarboxylation of indolepyruvate and subsequent oxidation of indoleacetaldehyde into indoleacetate, was purified from a thermoacidophilic archaeon, Sulfolobus sp. strain 7. The enzyme showed a M(r) of 280 kDa on gel filtration and was composed of three subunits (a, 89; b, 30; and c, 19 kDa), possibly in a stoichiometry of 2:2:2. Mo and Fe were detected. Thiamine pyrophosphate was absent. Biotin was suggested to bind to the b-subunit. The first step, the decarboxylation reaction, was specific for 2-oxoacids with an aromatic group, while in the second reaction, various aldehydes including glyceraldehyde, which is a glycolytic intermediate in the organism, were oxidized.
Wakagi
Takayoshi
T
Department of Biotechnology, The University of Tokyo, Japan. atwakag@mail.ecc.u-tokyo.ac.jp
Fukuda
Eriko
E
Ogawa
Yoko
Y
Kino
Hiroyasu
H
Matsuzawa
Hiroshi
H
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000302:isolation & purification; D001710:Biotin / Q000737:chemistry; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008982:Molybdenum / Q000737:chemistry*; D009097:Multienzyme Complexes / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D021122:Protein Subunits; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D016944:Sulfolobus / Q000201:enzymology*; D013696:Temperature
D019843:Archaeal Proteins; D007211:Indoles; D009097:Multienzyme Complexes; D021122:Protein Subunits; C445542:indolepyruvate - methylviologen oxidoreductase; C001655:indole-3-acetaldehyde; D001710:Biotin; D008982:Molybdenum; D010088:Oxidoreductases; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; C068832:indolepyruvate decarboxylase; D002262:Carboxy-Lyases