title: Influence of dehydrothermal crosslinking on the growth of PC-12 cells cultured on laminin coated collagen.
abstract: Recently, we have demonstrated canine peroneal nerve regeneration with functional recovery across an 80 mm gap using a polyglycolic acid (PGA) -collagen tube filled with laminin coated collagen fibers. In that study, the laminin coating was applied before a dehydrothermal (DHT) treatment designed to extend preservation of laminin in situ. To address concerns that the biological activity of laminin might consequently be reduced, the present investigation examined the influences of DHT crosslinking on the activity of laminin in terms of neural cell growth in vitro. DHT crosslinking was performed on collagen (type I or IV) spread on glass in three groups: (1) before coating, (2) after coating, and (3) both before and after coating. PC-12 cells were disseminated in each of the three groups. All three groups were cultured, and the number of cells were compared statistically. Cell growth achieved through application of laminin coating after DHT crosslinking was statistically greater than that achieved when laminin coating was performed before crosslinking. A reduction in laminin activity induced by DHT crosslinking was demonstrated. The optimal timing for the crosslinking of biornaterials treated with trophic factors such as-laminin should be examined in terms of the effects of crosslinking on the activity of the trophic factors.
authors.affiliation: Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Japan.
authors.forename: Toshinari
authors.initials: T
authors.lastname: Toba
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Seijun
authors.initials: S
authors.lastname: Fukuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Yoshitani
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ueda
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Hori
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology*; D024141:Collagen Type IV / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D011100:Polyglycolic Acid; D051381:Rats
chemical_list: D024042:Collagen Type I; D024141:Collagen Type IV; D003432:Cross-Linking Reagents; D007797:Laminin; D011100:Polyglycolic Acid