Suppressive effects of anti-allergic agent suplatast tosilate (IPD-1151T) on the expression of co-stimulatory molecules on mouse splenocytes in vivo.
The effects of IPD-1151T on the expression of co-stimulatory molecules, CD40, CD80 and CD86, were investigated in vivo using mice with allergic disorders. BALB/c mice were immunized intraperitoneally with two doses of dinitrophenylated ovalbumin (DNP-OVA) at 1-week intervals. These mice then were treated intraperitoneally with 100 microg/kg of IPD-1151T once a day for 14 days, starting 7 days after the first immunization. On day 21, some mice were challenged intraperitoneally with DNP-OVA and the other mice were not challenged. All mice were autopsied on day 22 and assayed for immunoglobulin E, interleuken (IL)-4 and IL-5 productions following DNP-OVA immunization. The intraperitoneal treatment with IPD-1151T strongly suppressed immunoglobulin E contents in serum, which were enhanced by DNA-OVA immunization. IPD-1151T also caused a decrease in both IL-4 and IL-5 levels in splenic lymphocytes. We next examined the influence of IPD-1151T on co-stimulatory molecule expression on splenic lymphocytes. IPD-1151T caused suppression of CD40 and CD86 expression; however, the treatments did not affect CD80 expression.
Kurokawa
M
M
First Department of Internal Medicine, Research Center for Medical Sciences School of Medicine, Showa University, Tokyo, Japan. cdl94140@par.odn.ne.jp
Kawazu
K
K
Asano
K
K
Fumio
K
K
Mita
A
A
Adachi
M
M
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis; D001190:Arylsulfonates / Q000494:pharmacology*; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000032:analysis; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000097:blood; D007165:Immunosuppression Therapy; D015847:Interleukin-4 / Q000032:analysis / Q000096:biosynthesis; D015848:Interleukin-5 / Q000032:analysis / Q000096:biosynthesis; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology*; D013452:Sulfonium Compounds / Q000494:pharmacology*
D018926:Anti-Allergic Agents; D015703:Antigens, CD; D001190:Arylsulfonates; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D015848:Interleukin-5; D008562:Membrane Glycoproteins; D013452:Sulfonium Compounds; D015847:Interleukin-4; D007073:Immunoglobulin E; C073028:suplatast tosilate