Antinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals.
In the present study, we have attempted to elucidate the active components for rheumatoidal arthritis using chloroform (CHCl(3)), ethylacetate (EtOAc) and n-butanol (BuOH) fractions of the methanol extract (MeOH) of Kalopanax pictus. Kalopanaxsaponin-A and -I (KPS-A and -I, hederagenin monodesmoside) were isolated from EtOAc fraction and kalopanaxsaponin-B, -H and -K (KPS-B, -H and -K, hederagenin bisdesmosides) obtained from BuOH fraction, respectively. MeOH extract, EtOAc fraction (250, 500 mg/kg, p.o.) and KPS-A and -I (5, 10, 20 mg/kg, i.p.) exhibited significant antinociceptive effects, which were determined by acetic acid-induced writhing test and hot plate test. On Freund's complete adjuvant reagent-induced rheumatoidal arthritis in rats, the administration of EtOAc fraction and KPS-A and -I inhibited edema, agglutination, vascular permeability and trypsin inhibitor. In addition, LD(50) of the MeOH extract was shown to be 4.033 mg/kg. These results suggest that anti-rheumatoidal effects of KPS-A and -I contribute to the inhibition of kinin formation by suppression of trypsin inhibitor activity.
Choi
Jongwon
J
College of Pharmacy, Kyungsung University, Pusan 608-736, South Korea.
Huh
Keun
K
Kim
Suk-Hwan
SH
Lee
Kyung-Tae
KT
Park
Hee-Juhn
HJ
Han
Yong Nam
YN
D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D018501:Antirheumatic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D027481:Araliaceae / Q000737:chemistry*; D001169:Arthritis, Experimental / Q000188:drug therapy; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010147:Pain Measurement / Q000187:drug effects / Q000706:statistics & numerical data; D008517:Phytotherapy / Q000379:methods / Q000706:statistics & numerical data; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*
D000700:Analgesics; D018501:Antirheumatic Agents; D010936:Plant Extracts; D012503:Saponins; C010177:beta-hederin; C112799:kalopanax saponin H; C117027:kalopanax saponin I; C064714:kalopanax saponin B; D009828:Oleanolic Acid