[The expression and clinical significance of pRB and E(2)F(1) in human neuroendocrine lung tumor].
OBJECTIVE
To investigate the role pRB and E(2)F(1) play in the cell cycle and the oncogenesis of human nemoendocrine lung fumor and the correlation between their expression and the invasiveness and prognosis of human neuroendocrine lung tumor.


METHODS
Immunohistochemistry and in situ hybridization were used to detect the expression of pRB and E(2)F(1) mRNA in the paraffin embedded tissues of 78 cases of human neuroendocrine lung tumor. Statistics was used to analyze the data.


RESULTS
The positive expression rate of pRB in both typical and atypical carcinoid was 87.5% (14/16). However, pRB was not expressed in large cell neuroendocrine carcinoma and small cell lung cancer (P < 0.001). The positive rate of E(2)F(1) mRNA expression in human neuroendocrine lung tumor was 87.2% (68/78). The strength of expression of pRB and E(2)F(1) was obviously correlated with the tumor stage, lymph node metastasis and prognosis (P < 0.05).


CONCLUSIONS
The loss of pRB and E(2)F(1) overexpression may play a key role in the development of human neuroendocrine lung tumors.
Tian
Y
Y
Thoracic Surgery Department of China-Japan Friendship Hospital, Beijing 100029, China.
Ge
B
B
Zhang
B
B
D000328:Adult; D000368:Aged; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018358:Neuroendocrine Tumors / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D016160:Retinoblastoma Protein / Q000032:analysis* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D014157:Transcription Factors