title: Expression of monosaccharide transporters in intestine of diabetic humans.
abstract: Noninsulin-dependent diabetes mellitus (NIDDM) is an increasingly common disease, which brings a number of life-threatening complications. In rats with experimentally induced diabetes, there is an increase in the capacity of the intestine to absorb monosaccharides. We have examined the activity and the expression of monosaccharide transporters in the intestine of patients suffering from NIDDM. Na(+)-dependent D-glucose transport was 3.3-fold higher in brush-border membrane (BBM) vesicles isolated from duodenal biopsies of NIDDM patients compared with healthy controls. Western analysis indicated that SGLT1 and GLUT5 protein levels were also 4.3- and 4.1-fold higher in diabetic patients. This was associated with threefold increases in SGLT1 and GLUT5 mRNA measured by Northern blotting. GLUT2 mRNA levels were also increased threefold in the intestine of diabetic patients. Analysis of other BBM proteins indicated that the activity and abundance of sucrase and lactase were increased by 1.5- to 2-fold and the level of the structural proteins villin and beta-actin was enhanced 2-fold in diabetic patients compared with controls. The increase in the capacity of the intestine to absorb monosaccharides in human NIDDM is due to a combination of intestinal structural change with a specific increase in the expression of the monosaccharide transporters SGLT1, GLUT5, and GLUT2.
authors.affiliation: Department of Veterinary Preclinical Sciences, The University of Liverpool, Brownlow Hill and Crown Street, Liverpool L69 7ZJ, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Dyer
authors.forename: I S
authors.initials: IS
authors.lastname: Wood
authors.forename: A
authors.initials: A
authors.lastname: Palejwala
authors.forename: A
authors.initials: A
authors.lastname: Ellis
authors.forename: S P
authors.initials: SP
authors.lastname: Shirazi-Beechey
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology; D004186:Disaccharidases / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000502:physiology; D051273:Glucose Transporter Type 2; D051296:Glucose Transporter Type 5; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D008840:Microfilament Proteins / Q000032:analysis; D008875:Middle Aged; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051269:Sodium-Glucose Transporter 1
chemical_list: D000199:Actins; D002352:Carrier Proteins; D051273:Glucose Transporter Type 2; D051296:Glucose Transporter Type 5; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495674:SLC5A1 protein, human; D051269:Sodium-Glucose Transporter 1; C026615:villin; D004186:Disaccharidases