Progression of actinic keratosis to squamous cell carcinoma of the skin correlates with deletion of the 9p21 region encoding the p16(INK4a) tumor suppressor.
Actinic keratoses (AKs) are pre-neoplastic lesions that can develop into squamous cell carcinomas (SCCs) of the skin. Often AK and SCC have commonly altered p53. A status of another tumor suppressor, the p16(INK4a), was reported for SCC but not for AK. A comparative study of SCC and AK human samples by loss of heterozygosity (LOH) analysis determined that the p16(INK4a/ARF) locus is less frequently altered in AKs than in SCCs. These LOH data highly correlated with immunohistochemical findings demonstrating the presence of p16(INK4a) in the AK skin samples but its absence in SCC lesions. Our results imply that progression of AK into SCC may involve inactivation of p16(INK4a).
Mortier
L
L
INSERM U459, Faculté de Médecine 1, Place de Verdun, Lille Cedex 59045, France.
Marchetti
P
P
Delaporte
E
E
Martin de Lassalle
E
E
Thomas
P
P
Piette
F
F
Formstecher
P
P
Polakowska
R
R
Danzé
P-M
PM
D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000209:etiology* / Q000235:genetics* / Q000378:metabolism; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D002899:Chromosomes, Human, Pair 9; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007642:Keratosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D012878:Skin Neoplasms / Q000209:etiology* / Q000235:genetics* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D019941:Cyclin-Dependent Kinase Inhibitor p16