title: Estrogen treatment of prostate cancer increases triglycerides in lipoproteins as demonstrated by HPLC and immunoseparation techniques.
abstract: BACKGROUND
  Estrogen administration is known to increase serum triglyceride concentrations. This study measured changes in lipoproteins of patients with prostate cancer treated with estrogen to determine whether the increased triglyceride concentrations are associated with atherogenic lipoprotein patterns.
  
  
  METHODS
  Fifteen patients (52-87 years) with histologically diagnosed prostate cancer received diethylstilbestrol diphosphate (250 mg/day). Serum samples were collected before and after 1 and 2 weeks of treatment. Cholesterol and triglyceride profiles of major lipoproteins were determined by HPLC, remnant-like particle cholesterol and triglyceride concentrations by an immunoseparation technique, and apolipoproteins by immunologic methods.
  
  
  RESULTS
  Estrogen treatment induced a 63.3% increase in total triglyceride concentrations, which occurred in all major lipoprotein classes with significant increases in HDL-triglycerides (130.4%), LDL-triglycerides (60.7%) and VLDL-triglycerides (56.2%). HDL-cholesterol increased significantly by 26.8%, while LDL-cholesterol decreased (15.6%). Remnant-like particle triglyceride concentrations also increased significantly by 77%, whereas remnant-like particle cholesterol concentrations remained unchanged. Apolipoproteins A-I and A-II increased; apolipoprotein E and Lp(a) decreased.
  
  
  CONCLUSIONS
  The techniques used here conveniently demonstrated that short-term estrogen treatment in prostate cancer patients resulted in triglyceride enrichment of all major lipoprotein classes but did not induce changes in the lipoprotein profiles generally recognized as increasing risk for cardiovascular disease, except for the elevation of plasma triglyceride and remnant-like particle triglyceride.
authors.affiliation: Department of Biochemistry and Biophysics, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Tokyo 113-8519, Bunkyo, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Usui
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Nakano
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Nakajima
authors.forename: Yukichi
authors.initials: Y
authors.lastname: Hara
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Okazaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001053:Apolipoproteins / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004054:Diethylstilbestrol / Q000031:analogs & derivatives / Q000627:therapeutic use*; D006801:Humans; D007163:Immunosorbent Techniques; D017270:Lipoprotein(a) / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*
chemical_list: D001053:Apolipoproteins; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017270:Lipoprotein(a); D008074:Lipoproteins; D014280:Triglycerides; D004054:Diethylstilbestrol; C004955:fosfestrol