Utilization of commercial non-chitinase enzymes from fungi for preparation of 2-acetamido-2-deoxy-D-glucose from beta-chitin.
Commercial non-chitinase enzymes from Aspergilus niger, Acremonium cellulolyticus and Trichoderma viride were investigated for potential utilization in the preparation of 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine, GlcNAc) from chitin. Among the tested enzymes, cellulase A. cellulolyticus exhibited highest chitinolytic activity per weight toward amorphous chitin and beta-chitin from squid pen. The optimum pH of the enzyme was 3 where it produced two major hydrolytic products, GlcNAc and N,N'-diacetylchitobiose ([GlcNAc](2)). The product ratio, GlcNAc:[GlcNAc](2), increased while the total yield decreased as the pH was raised from 3. All of the [GlcNAc](2) produced at pH 3 can be converted in situ to GlcNAc by mixing cellulase A. cellulolyticus with one of several other enzymes from A. niger resulting in a higher yield of GlcNAc. An appropriate mixing ratio of cellulase A. cellulolyticus to another enzyme was 9:1 (w/w) and an optimum substrate concentration was 20 mg/mL.
Sukwattanasinitt
Mongkol
M
Centre for Bioactive Compounds, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. mongkol@chula.ac.th
Zhu
Hong
H
Sashiwa
Hitoshi
H
Aiba
Sei-ichi
S
D000117:Acetylglucosamine / Q000378:metabolism*; D000164:Acremonium / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology; D002480:Cellulase / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D014242:Trichoderma / Q000201:enzymology*
D004798:Enzymes; D002686:Chitin; D002480:Cellulase; D000117:Acetylglucosamine