[Association of vascular endothelial growth factor expression with angiogenesis and tumor cell proliferation in human lung cancer].
OBJECTIVE
To evaluate the expression of vascular endothelial growth factor (VEGF) and the relation with angiogenesis and tumor cell proliferation in human lung cancer.


METHODS
Expression of VEGF, angiogenesis, tumor cell proliferation in 63 cases of human lung cancer were examined immunohistochemically. Anti-VEGF(165)-Ab3 was used for VEGF expression, a monoclonal antibody directed against CD(34) to identify intratumoral microvessel density (iMVD) and the proliferating cell nuclear antigen (PCNA) as a marker of proliferating tumor cells.


RESULTS
Positive staining for VEGF was obtained in 32 of the 63 cases (50.8%), iMVD varied from 4 to 138.7 (M = 36)/x 400 and the proportion of PCNA positive cells varied from 0 to 92% (M = 25.03%). iMVD in high-grade VEGF tumors was significantly higher than that in VEGF-negative tumors and low-grade VEGF tumors. PCNA labeling proliferation index in small cell lung cancer was higher than that in non-small cell lung cancer. iMVD significantly increased with increasing VEGF expression.


CONCLUSIONS
There is VEGF expression in lung cancer. VEGF expression is significantly associated with iMVD, but there is no association between iMVD or VEGF expression and PCNA expression. There is no association between VEGF expression and histological classification and clinical stages.
Ma
X
X
Respiratory Disease Institute, China Medical University, Shenyang 110001, China.
Zhang
L
L
Ma
H
H
Yu
R
R
D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A