Imaging of light emission from the expression of luciferases in living cells and organisms: a review.
Luciferases are enzymes that emit light in the presence of oxygen and a substrate (luciferin) and which have been used for real-time, low-light imaging of gene expression in cell cultures, individual cells, whole organisms, and transgenic organisms. Such luciferin-luciferase systems include, among others, the bacterial lux genes of terrestrial Photorhabdus luminescens and marine Vibrio harveyi bacteria, as well as eukaryotic luciferase luc and ruc genes from firefly species (Photinus) and the sea pansy (Renilla reniformis), respectively. In various vectors and in fusion constructs with other gene products such as green fluorescence protein (GFP; from the jellyfish Aequorea), luciferases have served as reporters in a number of promoter search and targeted gene expression experiments over the last two decades. Luciferase imaging has also been used to trace bacterial and viral infection in vivo and to visualize the proliferation of tumour cells in animal models.
Greer
Lee F
LF
Department of Biochemistry, School of Medicine and Department of Natural Sciences-Biology Section, Loma Linda University, Loma Linda, CA 92354, USA.
Szalay
Aladar A
AA
D000818:Animals; D002477:Cells / Q000166:cytology / Q000378:metabolism; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D049449:Luminescence; D030781:Organisms, Genetically Modified
D008156:Luciferases