title: Osteogenesis of large segmental radius defects enhanced by basic fibroblast growth factor activated bone marrow stromal cells grown on non-woven hyaluronic acid-based polymer scaffold.
abstract: Osteogenesis of large segmental radius defects in a rat model was studied by implanting a biodegradable non-woven hyaluronic acid-based polymer scaffold (Hyaff 11) alone or in combination with bone marrow stromal cells (BMSCs). These cells had been previously grown in vitro in mineralising medium either supplemented with basic fibroblast growth factor (bFGF) or unsupplemented. The healing of bone defects was evaluated at 40, 80, 160 and 200 days and the repair process investigated by radiographic, histomorphometric (assessment of new bone growth and lamellar bone) and histological analyses (toluidine blue and von Kossa staining). Mineralisation of bone defects occurred in the presence of the Hyaff 11 scaffold alone or when combined with BMSCs grown with or without bFGF, but each process had a different timing. In particular, bFGF significantly induced mineralisation from day 40, whereas 160 days were necessary for direct evidence that a similar process was developing under the other two conditions tested (scaffold alone or with BMSCs). Radiographic score, new bone growth and lamellar bone percentage were highly correlated. The present outcomes were further confirmed by toluidine blue and von Kossa staining. According to these in vivo findings, the Hyaff 11 scaffold is an appropriate carrier vehicle for the repair of bone defects; additionally, it can significantly accelerate bone mineralisation in combination with BMSCs and bFGF.
authors.affiliation: Laboratorio di Immunologia e Genetica, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Lisignoli
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: Aldini N
authors.initials: AN
authors.lastname: Nicoli
authors.forename: S
authors.initials: S
authors.lastname: Toneguzzi
authors.forename: A
authors.initials: A
authors.lastname: Facchini
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D017102:Fracture Healing; D006820:Hyaluronic Acid / Q000031:analogs & derivatives*; D066298:In Vitro Techniques; D008297:Male; D010012:Osteogenesis / Q000502:physiology*; D011108:Polymers; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D016222:Fibroblast Growth Factor 2; C086358:hyaluronic acid benzyl ester; D006820:Hyaluronic Acid