Physical exercise induces activation of NF-kappaB in human peripheral blood lymphocytes.
Current understanding of nuclear factor-kappaB (NF-kappaB) activation is derived mostly from in vitro studies, and in vivo human data are limited. This study provides first evidence showing that physical exercise (80% maximal O2 consumption, 1 h) may trigger NF-kappaB activation, as determined by electrophoretic mobility shift assay, in peripheral blood lymphocytes of physically fit young men. Supershift assay showed that the NF-kappaB protein complex contained the transcriptionally active p65 protein. Plasma levels of NF-kappaB-directed gene products such as tumor necrosis factor-alpha and interleukin-2 receptor confirmed that physical exercise caused NF-kappaB transactivation. Exercise-induced NF-kappaB activation in lymphocytes was associated with elevated levels of lipid peroxidation by-products in the plasma.
Vider
J
J
Department of Surgery, Davis Heart & Lung Research Institute, The Ohio State University Medical Center, Columbus, USA. Jelena.Vider@kliinik.um.ee
Laaksonen
D E
DE
Kilk
A
A
Atalay
M
M
Lehtmaa
J
J
Zilmer
M
M
Sen
C K
CK
D000328:Adult; D015444:Exercise; D006801:Humans; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000097:blood*; D015375:Receptors, Interleukin-2 / Q000097:blood; D013997:Time Factors; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016328:NF-kappa B; D015375:Receptors, Interleukin-2; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha