[Evaluation on the cytotoxicity of Gallium alloy by MTT-assay].
OBJECTIVE
To test the cytotoxicity of Gallium alloy, a new mercury-free dental restorative material.


METHODS
L-929 mouse fibroblasts were used to detect the cell relative proliferation rate of Gallium alloy and high-copper amalgam by MTT-assay.


RESULTS
The results indecated that the relative growth rate induced by Gallium alloy was high (93.0% +/- 4.9% 2ds, 102.0% +/- 3.5% 4ds, 107.2% +/- 4.2% 7ds), and the cytotoxicity of Gallium alloy was 0 grade according to the Test Standard of Shanghai Medical Biomaterial, meaning Gallium alloy had no significant cytotoxicity and the relative growth rate of Gallium alloy was higher than that of high-copper amalgam. The high-copper amalgam showed medium cytotoxicity.


CONCLUSIONS
Gallium alloy has no significant cytotoxicity.
Xu
G
G
Department of Orthodontics, Stomatology Hospital of Zhejiang University, Hangzhou 310006, China.
Zhang
C
C
Ning
L
L
D000497:Alloys / Q000633:toxicity*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005708:Gallium / Q000633:toxicity*; D051379:Mice; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D000497:Alloys; C110328:Metallodent-C; D013778:Tetrazolium Salts; D013844:Thiazoles; D005708:Gallium; C022616:thiazolyl blue