Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography.
A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH2PO4-acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (Haliotis rufescens) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2+/-0.9% extraction efficiency.
Viant
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MR
Department of Environmental Toxicology, University of California, Davis 95616, USA. mrviant@ucdavis.edu
Rosenblum
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ES
Tjeerdema
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D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008974:Mollusca / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis*; D015203:Reproducibility of Results
D009943:Organophosphorus Compounds; C015441:phospho-L-arginine; D001120:Arginine