Electrochemically mediated electrodeposition/electropolymerization to yield a glucose microbiosensor with improved characteristics.
A procedure is described that provides for electrochemically mediated deposition of enzyme and a polymer layer permselective for endogenous electroactive species. Electrodeposition was first employed for the direct immobilization of glucose oxidase to produce a uniform, thin, and compact film on a Pt electrode. Electropolymerization of phenol was then employed to form an anti-interference and protective polyphenol film within the enzyme layer. In addition, a stability-reinforcing membrane derived from (3-aminopropyl)trimethoxysilane was constructed by electrochemically assisted cross-linking. This hybrid film outside the enzyme layer contributed to the improved stability and permselectivity. The resulting glucose sensor was characterized by a short response time (<4 s), high sensitivity (1200 nA/mM x cm2), low interference from endogenous electroactive species, and working lifetime of more than 50 days.
Chen
Xiaohong
X
Department of Chemistry, University of Kansas, Lawrence 66045, USA.
Matsumoto
Norio
N
Hu
Yibai
Y
Wilson
George S
GS
D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000592:standards; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D008904:Miniaturization; D011108:Polymers
D004800:Enzymes, Immobilized; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose