title: Pulmonary surfactant and inflammation in septic adult mice: role of surfactant protein A.
abstract: Surfactant alterations, alveolar cytokine changes, and the role of surfactant protein (SP)-A in septic mice were investigated. Sepsis was induced via cecal ligation and perforation (CLP). Septic and sham mice were euthanized at 0, 3, 6, 9, 12, 15, and 18 h after surgery. Mice deficient in SP-A and mice that overexpressed SP-A were euthanized 18 h after surgery. In wild-type, sham-operated mice, surfactant pool sizes were similar at all time points, whereas in the CLP groups there was a significant decrease in small-aggregate surfactant pool sizes beginning 6 h after CLP. Interleukin-6 concentrations in bronchoalveolar lavage fluid from septic animals increased from 6 to 18 h after surgery. Identical surfactant alterations and concentrations of cytokines were observed in septic mice that were SP-A deficient or that overexpressed SP-A. In conclusion, alterations of pulmonary surfactant and alveolar cytokines occur simultaneously, 6 h after a systemic insult. In addition, we did not detect a role for SP-A in regulating surfactant phospholipid pool sizes or pulmonary inflammation in septic mice.
authors.affiliation: Department of Physiology, Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada N6A 4V2.
authors.forename: Jaret L
authors.initials: JL
authors.lastname: Malloy
authors.forename: Ruud A W
authors.initials: RA
authors.lastname: Veldhuizen
authors.forename: Francis X
authors.initials: FX
authors.lastname: McCormack
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Korfhagen
authors.forename: Jeffery A
authors.initials: JA
authors.lastname: Whitsett
authors.forename: James F
authors.initials: JF
authors.lastname: Lewis
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D009994:Osmolar Concentration; D011014:Pneumonia / Q000382:microbiology* / Q000503:physiopathology*; D011510:Proteolipids / Q000235:genetics / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D012016:Reference Values; D013997:Time Factors
chemical_list: D016207:Cytokines; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants