Attachment of benzaldehyde-modified oligodeoxynucleotide probes to semicarbazide-coated glass.
Attachment of oligodeoxynucleotides (ODNs) containing benzaldehyde (BAL) groups to semicarbazide-coated glass (SC-glass) slides is described. 5'-BAL-ODNs are prepared using automated DNA synthesis and an acetal-protected BAL phosphoramidite reagent. The hydrophobic protecting group simplifies purification of BAL-ODNs by reverse phase HPLC and is easily removed using standard acid treatment. The electrophilic BAL-ODNs are stable in solution, but react specifically with semicarbazide groups to give semicarbazone bonds. Glass slides were treated with a semicarbazide silane to give SC-glass. BAL-ODNs are coupled to the SC-glass surface by a simple one-step procedure that allows rapid, efficient and stable attachment. Hand-spotted arrays of BAL-ODNs were prepared to evaluate loading density and hybridization properties of immobilized probes. Hybridization to radiolabeled target strands shows that at least 30% of the coupled ODNs were available for hybridization at maximum immobilization density. The array was used to probe single nucleotide polymorphisms in synthetic DNA targets, and PCR products were correctly genotyped using the same macroarray. Application of this chemistry to manufacturing of DNA microarrays for sequence analysis is discussed.
Podyminogin
M A
MA
Epoch Biosciences, 21720 23rd Drive SE 150, Bothell, WA 98021, USA.
Lukhtanov
E A
EA
Reed
M W
MW
D001547:Benzaldehydes / Q000737:chemistry*; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D005898:Glass; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000235:genetics; D012680:Sensitivity and Specificity
D001547:Benzaldehydes; D015342:DNA Probes; D009841:Oligonucleotides; C032175:benzaldehyde