Capillary electrophoresis-electrospray mass spectrometry for the characterization of high-mannose-type N-glycosylation and differential oxidation in glycoproteins by charge reversal and protease/glycosidase digestion.
The characterization of high-mannose-type N-glycosylation by capillary electrophoresis-electrospray mass spectrometry (CE-ESI MS) was described. In addition to the use of a cationic noncovalent capillary coating, strong acidic buffer, and charge reversal to increase the glycoform resolving power, N-glycosidase F (PNGase F) combined with a basic protease and alpha-mannosidase combined with an acidic protease were used to analyze the high-mannose-type N-glycosylation in ribonuclease B (RNase B) and in a novel C-type lectin from the venom of Trimeresurus stejnegeri (TSL). The structures of oligosaccharide, glycosylation sites, and glycoform distributions were determined simultaneously, and the differential oxidation of Met residues in glycopeptides obtained from TSL protease digestion was also characterized successfully by CE-MS/MS. The results showed that the oligosaccharide attached to RNase B has a structure of GlcNAc2Man5 approximately 9, and that attached to TSL has a structure of GlcNAc2Min5 approximately 8. The glycoform distributions in these glycoproteins are quite different, with the GlcNAc2Man5 type predominant in RNase B, and the GlcNAc2Man8 type, in TSL This method may be useful not only for the characterization of glycosylation sites and glycan structures, but also for the determination of the relative abundance of individual glycoforms.
Liu
T
T
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, People's Republic of China.
Li
J D
JD
Zeng
R
R
Shao
X X
XX
Wang
K Y
KY
Xia
Q C
QC
D000478:Alkylation; D000595:Amino Acid Sequence; D000818:Animals; D019075:Electrophoresis, Capillary; D010450:Endopeptidases; D006023:Glycoproteins / Q000737:chemistry*; D006026:Glycoside Hydrolases; D006031:Glycosylation; D006868:Hydrolysis; D037102:Lectins / Q000737:chemistry; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010449:Peptide Mapping; D012260:Ribonucleases / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D017841:Trimeresurus
D006023:Glycoproteins; D037102:Lectins; D012260:Ribonucleases; C029600:ribonuclease B; D006026:Glycoside Hydrolases; D010450:Endopeptidases; D008358:Mannose