[Expression of C3 epitope of poliovirus and a ten-peptides epitope of C-myc on the surface of recombinant bacteria].
To confirm the possibility of pCSB136 and pCSX72 as vectors for displaying heterologous epitopes, the gene fragments coding for C3 epitope of poliovirus and a ten-peptides epitope of C-myc were synthesized and inserted into pCSB136 and pCSX72 respectively. The recombinants were screened by whole-strain PCR. The expression of recombinant proteins were detected by whole-cell ELISA and electronic microscopy. The results indicated the recombinant proteins were expressed as hybrid fimbriae, and the antigenicity of both CS3 and inserted epitopes kept. All results above showed vectors pCSB136 and pCSX72 could be used to display the foreign epitopes.
Gao
R K
RK
Beijing Institute of Biotechnology, Beijing 100071, China.
Zhang
Z S
ZS
Li
S Q
SQ
Huang
C F
CF
D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008854:Microscopy, Electron; D017955:Poliovirus / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D036022:Capsid Proteins; D000939:Epitopes; D008565:Membrane Proteins; D011994:Recombinant Proteins; C442540:VP1 protein, Poliovirus