title: Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.
abstract: Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
authors.affiliation: Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany. felix.rodich@ch.tum.de
authors.forename: Felix
authors.initials: F
authors.lastname: Rohdich
authors.forename: Stefan
authors.initials: S
authors.lastname: Hecht
authors.forename: Katrin
authors.initials: K
authors.lastname: Gärtner
authors.forename: Petra
authors.initials: P
authors.lastname: Adam
authors.forename: Cornelia
authors.initials: C
authors.lastname: Krieger
authors.forename: Sabine
authors.initials: S
authors.lastname: Amslinger
authors.forename: Duilio
authors.initials: D
authors.lastname: Arigoni
authors.forename: Adelbert
authors.initials: A
authors.lastname: Bacher
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eisenreich
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D004755:Enterobacteriaceae / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D009682:Magnetic Resonance Spectroscopy; D008798:Mevalonic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009876:Operon; D010088:Oxidoreductases; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D013729:Terpenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D013729:Terpenes; D010088:Oxidoreductases; C109866:ispH protein, E coli; D008798:Mevalonic Acid