title: Linkage of sugar chains to a fragment peptide of FGF-5S by a chemoenzymatic strategy and changes in the rate of proteolytic hydrolysis.
abstract: Various O-linked and N-linked sugar chains were linked enzymatically to a fragment peptide (Leu-Ser-Gln(or Asn)-Val-His-Arg) of FGF-5S. First, galactose was linked with beta-(1-->3)-linkage to GalNAc-linked peptide by a transglycosylation using beta-galactosidase from Bacillus circulans (recombinant). Then sialic acid was linked with the aid of sialyltransferase from rat liver (recombinant) to give NeuAcalpha-(2-->3)-Galbeta-(1-->3)-GalNAc-linked hexapeptide. Further, a sialylated 2-chain biantennary sugar chain was linked by a transglycosylation using endo N-acetyl-beta-D-glucosaminidase from Mucor hiemalis (endo M, recombinant). The activity of DNA synthesis in a fibroblast cell line was increased by this glycosylation. The resistance of the obtained glycopeptides towards proteolytic hydrolysis by rat serum and by five proteases was compared with that of original peptide. The resistance was remarkably enhanced by the glycosylation.
authors.affiliation: Nutrition Science Institute, Meiji Milk Products Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan. kajisaka@mbc.sphere.ne.jp
authors.forename: K
authors.initials: K
authors.lastname: Ajisaka
authors.forename: M
authors.initials: M
authors.lastname: Miyasato
authors.forename: C
authors.initials: C
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: Y
authors.initials: Y
authors.lastname: Yamazaki
authors.forename: S
authors.initials: S
authors.lastname: Oka
mesh_terms: D000818:Animals; D001407:Bacillus; D002851:Chromatography, High Pressure Liquid; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors / Q000737:chemistry* / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D006868:Hydrolysis; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D012799:Sialyltransferases / Q000378:metabolism; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C496451:Fgf5 protein, rat; D006023:Glycoproteins; D010446:Peptide Fragments; D011134:Polysaccharides; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors; D012799:Sialyltransferases; D001616:beta-Galactosidase; D010447:Peptide Hydrolases