title: DNAinsight: a web based image processing system for large scale RLGS analysis.
abstract: DNA methylation occurring within the context of CpG site in the promoter element generally correlates with transcriptional silencing, delayed replication, condensed chromatin and mammalian genome imprinting (Nature Genet 24:101-2, 2000). We have been focusing attention on Restriction Landmark Genomic Scanning (RLGS) method as the powerful experimental procedure which can used to determine DNA methylation status change occurring on a genome simultaneously. To support systematic analysis with RLGS method, we have developed the automated processing algorithms for two-dimensional electrophoretograms of genomic DNA based on RLGS method (RLGS profile). Our powerful processing algorithms realize the automated spot recognition from RLGS profiles and the automated comparison of a huge number of such profiles. To make systematic RLGS analysis more easy and objective, we equipped our automated RLGS image processing algorithms with a WWW interface and relational database which stores the RLGS profile images together with their accompanying information, such as DNA sample specification, two-dimensional electrophoresis conditions and so forth. The web based RLGS image processing system "DNAinsight" can be accessed via URL http://www.methylome.jp/DNAinsight/.
authors.affiliation: Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Aomi 2-41-6, Koutou-ku, Tokyo 135-0064, Japan. takahashi-k@aist.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000465:Algorithms; D019295:Computational Biology; D018899:CpG Islands; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D019175:DNA Methylation; D030561:Databases, Nucleic Acid; D015180:Electrophoresis, Gel, Two-Dimensional; D015894:Genome, Human; D023281:Genomics / Q000706:statistics & numerical data*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020407:Internet
chemical_list: D004247:DNA