title: Cryopreservation of Taxus chinensis suspension cell cultures.
abstract: A simple cryopreservation method for suspension cells of Taxus chinensis was established. In this procedure 7 days old suspension cells were used without any pre-culture treatment. At first, cells were incubated in cryoprotectant solution (0.5M DMSO and 0.5M glycerol) on ice for 30 min and then frozen at a cooling rate of 1 degree C/min to -40 degrees C prior to immersion in liquid nitrogen. The average viability of frozen-thawed cells was between 30 to 40%. The recovery of cryopreserved cells in liquid nitrogen for 1 month was accomplished. After rapid thawing, cells were transferred to solid medium and cultivated for 4-6 weeks. The treatment of trehalose as a cryoprotectant enhanced re-growth of frozen-thawed cells. The stable maintenance of paclitaxel biosynthetic ability in cryopreserved cells was confirmed by comparing with that of regularly sub-cultured suspension cells.
authors.affiliation: Samyang Genex Biotech Research Institute,63-2, Hwaam-Dong, Yusung-Gu, Taejon, 305-348, Korea.
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
authors.forename: H K
authors.initials: HK
authors.lastname: Choi
authors.forename: J S
authors.initials: JS
authors.lastname: Son
authors.forename: J H
authors.initials: JH
authors.lastname: Yun
authors.forename: M S
authors.initials: MS
authors.lastname: Jang
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Choi
authors.forename: S S
authors.initials: SS
authors.lastname: Hong
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation; D003451:Cryoprotective Agents; D017694:Rewarming; D020946:Taxus / Q000166:cytology*
chemical_list: D003451:Cryoprotective Agents