title: Interdigital apoptosis and downregulation of BAG-1 expression in mouse autopods.
abstract: Digit formation during vertebrate limb development is a well-known example of programmed cell death. We have used this system to analyze whether the formation of the interdigital necrotic zone in mouse autopods is linked with the expression of BAG-1, a gene with an anti-death activity. Here, we demonstrate that during development of mouse autopods, BAG-1 expression is downregulated upon the initiation of interdigital apoptosis. We further show that retinoic acid induced interdigital apoptosis is also correlated with a downregulation of BAG-1 expression. On the contrary, the expression of BAG-1 remains unaltered in autopods of RARbeta(-/-)/RARgamma(-/-) mice which show severe interdigital webbing due to a marked decrease in interdigital apoptosis.
authors.affiliation: Forschungszentrum Karlsruhe, Institute of Toxicology and Genetics, P.O. Box 3640, 76021, Karlsruhe, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Crocoll
authors.forename: Ute
authors.initials: U
authors.lastname: Herzer
authors.forename: Norbert B
authors.initials: NB
authors.lastname: Ghyselinck
authors.forename: Pierre
authors.initials: P
authors.lastname: Chambon
authors.forename: Andrew C B
authors.initials: AC
authors.lastname: Cato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D002352:Carrier Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins; D015536:Down-Regulation; D005121:Extremities / Q000196:embryology* / Q000473:pathology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors; D014212:Tretinoin / Q000494:pharmacology; D000097768:Retinoic Acid Receptor gamma
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors; D014212:Tretinoin; D000097768:Retinoic Acid Receptor gamma; C091732:BCL2-associated athanogene 1 protein; C082925:retinoic acid receptor beta