Present situation of serotyping of Pseudomonas aeruginosa in Japan and correlation among three kinds of commercially available serotyping kits.
We carried out the serotyping of 196 freshly isolated strains of Pseudomonas aeruginosa with international antigenic typing system (IATS) antibodies from Rougier Bio-Tech (RBT kit) and other two kinds of antibodies, from Meiji Seika Kaisha (Mei assay kit) and Denka Seiken (Seiken kit) to ascertain the relationship among the serotypes obtained by the different methods. All the test strains belonging to the serogroups A, C, E, F, G, H, I, J, and K were well correlated with each other with the Mei assay and the Seiken kits. However, 21 of 26 strains of serogroup M typed with the Mei assay kit were non-typable with the Seiken kit. It was confirmed that serogroups obtained with both these methods were closely related; 171 of the 196 strains of P. aeruginosa tested (87.2%) were classified into identical serogroups. From the serotyping results for P. aeruginosa strains with the Seiken and the RBT kits and with the Mei assay and the RBT kits, it was found that the serotypes of 172 of the 192 strains tested (89.6%) and those of 164 of the 170 strains tested (96.5%), respectively were well correlated. However, the serotyping of P. aeruginosa with the RBT kit may be problematic, as the kit did not contain the antisera agglutinated with the serogroup M strains of P. aeruginosa classified with the Mei assay and Seiken kits.
Goto
S.
S
College of Health Professions, Toho University, 4-16-20 Omorinishi, Ota-ku, Tokyo 143-0015, Japan.
Tsuji
A.
A
Oguri
T.
T
Kobayashi
I.
I
Nishida
M.
M
Yabuuchi
E.
E