title: Preparation strategy for uniformly sized, polymer-based HPLC packing materials having practically acceptable column efficiency. 1. Copolymerization technique.
abstract: Uniformly sized, polymer-based packing materials affording excellent chromatographic performance in semimicro HPLC were prepared from mixtures of alkyl methacrylate and glycerol dimethacrylate by a copolymerization technique using a multistep swelling and polymerization method. A column efficiency of up to 13000 plates was obtained for a 2-mm-i.d. x 150-mm column with beads prepared from a 1:1 (v:v) mixture of an alkyl methacrylate and glycerol dimethacrylate. The packing materials exhibit a considerable improvement in column efficiency, as demonstrated on the separation of polyaromatic hydrocarbons (PAHs) having a relatively long retention time, which thus far has been a serious problem of polymer-based packing materials. Detailed studies suggested a rather limited optimum pore size, and its distribution was depicted to get the excellent column efficiency on polymer-based packing materials.
authors.affiliation: Department of Polymer Science, Kyoto Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hosoya
authors.forename: M
authors.initials: M
authors.lastname: Teramachi
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Kanda
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsu