The potential of 19F NMR spectroscopy for rapid screening of cell cultures for models of mammalian drug metabolism.
The use of microbial cultures as a complementary model for mammalian drug metabolism has been well established previously. Here is a preliminary investigation into the potential of 19F NMR spectroscopy as a rapid screening tool to quantify the biotransformations of fluorine-containing model drugs. Biotransformations of three model drugs in 48 taxonomically diverse organisms were measured by acquiring 19F NMR spectra at 376 MHz. The presence of fluorine in the molecules allowed rapid, simultaneous detection of over 20 biotransformation products without sample pretreatment, chromatography, mass spectrometric techniques or the use of radiolabelled substrates. The detection limit at 376 MHz using 5 mm NMR tubes was ca. 0.3 microg ml(-1) using a typical analysis time of 20 min per sample. With the recent advent of flow injection NMR technology, analysis time of 5 min could be achieved with less sample. This approach may be used to develop fast small-scale microbial screens for the biosynthesis of metabolite standards and production of novel drug analogues, whilst also having a role in reducing animal experiments needed to identify animal and human metabolites of fluorinated xenobiotics.
Corcoran
O
O
Division of Biomedical Sciences, Imperial College of Science, Technology and Medicine, London, UK. olivia.corcoran@bruker-biospin.com
Lindon
J C
JC
Hall
R
R
Ismail
I M
IM
Nicholson
J K
JK
D000818:Animals; D001431:Bacteriological Techniques; D005461:Fluorine; D009682:Magnetic Resonance Spectroscopy; D008322:Mammals / Q000378:metabolism*; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*
D004364:Pharmaceutical Preparations; D005461:Fluorine