[Effects of bushen yizhi recipe on somatostatin-like immunopositive and somatostatin messenger ribonucleic acid expressed-positive neurons in Alzheimer's disease model rats].
OBJECTIVE
To observe the effect of Bushen Yizhi Recipe (BYD) on somatostatin-like (SS-like) immuno-reactivity and SS mRNA expressed-positive neurons in Alzheimer disease (AD) model rats induced by D-galactose intraperitoneal and ibotenic acid (IBO) intracerebral injection.


METHODS
Fifteen months Wistar rats were randomly divided into 4 groups: normal control; AD model control induced by D-galactose intraperitoneal injection and lesion of basal nucleus of Meynert with IBO; Huperzine A (Hup-A) treated group and BYD treated group fed with Hup-A or BYD respectively. The SS-like immunoreactivity and SS mRNA expressed-positive neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in the brains of rats, which were detected with immunohistochemistry and DIG-labelling cRNA-probe in situ hybridization.


RESULTS
Counts and integrations of optic density of SS-like immuno-reactivity and SS-mRNA expressed neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in BYD treated group increased compared with AD model rats.


CONCLUSIONS
BYD might protect the SS-ergic system against impairment of D-galactose and IBO in the brains of the AD model rats.
Hu
J
J
Guangzhou University of Traditional Chinese Medicine, Guangzhou (510405).
Wang
Q
Q
Liang
W
W
D000544:Alzheimer Disease / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005690:Galactose; D007051:Ibotenic Acid; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C000604209:bushen yizhi; D007051:Ibotenic Acid; D013004:Somatostatin; D005690:Galactose