[The protein expression of insulin receptor substrate-1 and protein tyrosine phosphatase with two src-homology II in the adipose tissue from type II diabetic patients].
OBJECTIVE
To investigate the protein expression of insulin receptor substrate-1 (IRS-1), protein tyrosine phosphatase with two src-homology 2 (SH-PTP(2)) in adipose tissues of type II diabetic patients, and explore molecular mechanisms of insulin resistance from adipose tissues of type II diabetic individuals.


METHODS
The levels of protein expression of IRS-1 and SH-PTP(2) in adipose tissues from type II diabetic individuals were measured by Western blot analysis; meanwhile compared the level of protein expression of one's own abdominal subcutaneous and omentum majus adipose tissue from patients with type II diabetes mellitus.


RESULTS
The expression of IRS-1 protein in abdominal subcutaneous (A) 2.14 +/- 0.67 and omentum majus 3.25 +/- 0.70 adipose tissues from patients with type II diabetes mellitus was significantly lower than that of control group [subcutaneous: 4.33 +/- 0.57 (P < 0.001), omentum majus: 8.65 +/- 2.85 (P < 0.05)]. The expression of SH-PTP(2) protein had no difference compared with control group (P > 0.05). The protein expression of IRS-1 was significantly lower in abdominal subcutaneous adipose tissue (1.09 +/- 0.13) than that in omentum majus adipose tissue (2.10 +/- 0.22) from the type II diabetic patients (P < 0.05), but the protein expression of SH-PTP(2) was significantly higher in subcutaneous adipose tissue (70.75 +/- 2.18) than that in omentum majus (43.69 +/- 11.07, P < 0.05).


CONCLUSIONS
The abnormal changes in expression of IRS-1 and SH-PTP(2) protein in adipose tissues of the patients with type II diabetic mellitus may be one of the mechanisms of leading to insulin resistance. The abdominal subcutaneous adipose tissue of type II diabetic patients, as visceral adipose tissues, contributes to their insulin resistance.
Peng
D
D
Department of Endocrinology, the General Hospital of Civil Aviation Administration of China, Beijing 100025, China.
Gao
Y
Y
D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000096:biosynthesis*; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D018909:src Homology Domains
C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D010750:Phosphoproteins; D017027:Protein Tyrosine Phosphatases