title: Human non-pregnancy ribonuclease with anti-Kaposi's sarcoma activity.
abstract: Our demonstration of a 19kDa anti-Kaposi's sarcoma (KS) ribonuclease (RNase) in urine from a non-pregnant female may provide at least part of the explanation for the low incidence of KS in human females. N-terminal sequence analysis and isoelectric focusing of the purified RNase, coupled with the very low levels of anti-KS activity noted for recombinant forms of human eosinophil derived neurotoxin and human eosinophil cationic protein, suggest that the 19kDa enzyme is an eosinophilic protein whose potent anti-KS activity is dependent upon post-translational modifications that occur only in human cells.
authors.affiliation: Division of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Moza M A
authors.initials: MM
authors.lastname: Al-Owais
authors.forename: Lars
authors.initials: L
authors.lastname: Hesse
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Talbot
authors.forename: David J
authors.initials: DJ
authors.lastname: Adams
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D047091:Eosinophil Granule Proteins; D047208:Eosinophil-Derived Neurotoxin; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry* / Q000235:genetics / Q000652:urine*; D012514:Sarcoma, Kaposi / Q000188:drug therapy* / Q000201:enzymology*; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D011994:Recombinant Proteins; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases