Ras target protein canoe is a substrate for Cdc2 and Cdk5 kinases.
Mutations in the canoe locus of Drosophila lead to failure in the dorsal closure of the embryonic epidermis and pattern formation defects in imaginal eyes and wings. In the wing, the canoe mutants develop extra veins when they are heterozygous for shaggy, a mutation in the locus encoding the glycogen synthase kinase 3 beta (Gsk3 beta), which has been known to phosphorylate the Armadillo protein. Although Canoe has a putative target sequence for phosphorylation by Gsk3 beta similar to that found in Armadillo, in vitro experiments indicate that Canoe is not phosphorylated by Gsk3 beta . Instead, Canoe is demonstrated to be a good substrate of Cdc2 and Cdk5 kinases. Thus, Cdc2 and Cdk5 kinases are the potential regulators of the function of Canoe in morphogenesis. Arch.
Takahashi
Kuniaki
K
ERATO Yamamoto Behavior Genes Project, JST, Mitsubishikagaku Institute of Life Sciences, Machida, Japan.
Hamada
Noriko
N
Yamamoto
Daisuke
D
D000595:Amino Acid Sequence; D000818:Animals; D051186:Armadillo Domain Proteins; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002417:Cattle; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015534:Trans-Activators; D014157:Transcription Factors; D018631:ras Proteins / Q000378:metabolism*
C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C092747:cno protein, Drosophila; D038341:Glycogen Synthase Kinases; D051360:Cyclin-Dependent Kinase 5; D017346:Protein Serine-Threonine Kinases; C080335:Sgg protein, Drosophila; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase; C495904:Cdk5 protein, Drosophila; D018844:Cyclin-Dependent Kinases; D038362:Glycogen Synthase Kinase 3; D018631:ras Proteins