Comparative effects of eugenol to bis-eugenol on oral mucous membranes.
The purpose of this study was to evaluate the histopathological effect of eugenol and bis-eugenol on oral mucous membranes at the tissue organ level. Oral mucous membranes of mice were applied with three reagents, eugenol, bis-eugenol, and acetone (as the control). The control group showed a normal architecture. The eugenol group showed severe hyperkeratosis, parakeratosis, cellular edema, patchy chronic inflammation, pleomorphism and hyperchromatism of basal layer cells, indicating high mitotic activity. Comparatively, the bis-eugenol group showed mild hyperkeratosis, parakeratosis, however, the shape or arrangement of basal layer cells were normal. Bis-eugenol was considerably less toxic than eugenol.
Fujisawa
S
S
Department of Oral Diagnosis, School of Dentistry, Meikai University, 1-1 Keyakidai, Sakado, Saitama 350-0283, Japan.
Okada
N
N
Muraoka
E
E
D000096:Acetone / Q000633:toxicity; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D048429:Cell Size / Q000187:drug effects; D003764:Dental Materials / Q000633:toxicity*; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005054:Eugenol / Q000633:toxicity*; D015603:Keratinocytes / Q000187:drug effects / Q000473:pathology; D007642:Keratosis / Q000139:chemically induced / Q000473:pathology; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D009061:Mouth Mucosa / Q000187:drug effects* / Q000473:pathology; D013280:Stomatitis / Q000139:chemically induced / Q000473:pathology
D001672:Biocompatible Materials; D003764:Dental Materials; D000096:Acetone; D005054:Eugenol