title: Immobilization of biomolecules on polyurethane membrane surfaces.
abstract: Lipophilic polymer membranes incorporating binding sites are widely used in various potentiometric, amperometric, and optical sensors. Here, we report on the biofunctional modification of the surface of a Ca(2+)-selective membrane. A photoactivatable biotin derivative was synthesized and covalently immobilized on a soft polyurethane membrane. The modified polymer was characterized by X-ray photoelectron spectroscopy (XPS) as well as by potentiometric measurements. The selective binding of streptavidin by the photo-cross-linked biotin derivative was demonstrated. The surface coverage obtained with different experimental protocols was analyzed by autoradiography using [(35)S]-streptavidin. The new approach may significantly extend the scope of applicability of potentiometric sensors.
authors.affiliation: Institute of Microtechnology (IMT), University of Neuchâtel, Rue Jaquet-Droz 1, CH-2007 Neuchâtel, Switzerland.
authors.forename: Patrick
authors.initials: P
authors.lastname: Reichmuth
authors.forename: Hans
authors.initials: H
authors.lastname: Sigrist
authors.forename: Martin
authors.initials: M
authors.lastname: Badertscher
authors.forename: Werner E
authors.initials: WE
authors.lastname: Morf
authors.forename: Nicolaas F
authors.initials: NF
authors.lastname: de Rooij
authors.forename: Ernö
authors.initials: E
authors.lastname: Pretsch
mesh_terms: D001345:Autoradiography; D004566:Electrodes; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D010777:Photochemistry; D011140:Polyurethanes / Q000138:chemical synthesis* / Q000737:chemistry; D011199:Potentiometry; D012997:Solvents; D013052:Spectrometry, X-Ray Emission; D019809:Streptavidin / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011140:Polyurethanes; D012997:Solvents; D019809:Streptavidin