[Expression of rat amelogenin gene in Escherichia coli].
OBJECTIVE
To detect the expression of amelogenin (Am) gene in Escherichia coli (E. coli).


METHODS
The cDNA fragmain of Am gene was obtained with EcoRI and Xhol I from the plasmid PUC18/Am. The fragment was inserted into prokaryotic gene fusion vector PGEX-4T-2 and an expression plasmid PGEX-4T-2/Am was constructed. PGEX-4T-2/Am was induced by IPTG for 4 h.


RESULTS
15% SDS-PAGE revealed a new forein protein band near 27,000.


CONCLUSIONS
The constructed plasmid expresses Am gene in E. coli.
Sui
W
W
Department of Conservative Dentistry, College of Stamatology, Forth Military Medical University, Xi'an 710032, China.
Zhang
X
X
Liu
X
X
D053523:Amelogenin; D000818:Animals; D003746:Dental Enamel Proteins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis*
D053523:Amelogenin; C507201:Amelx protein, rat; D003746:Dental Enamel Proteins; D011994:Recombinant Proteins