[Protective immunity induced by anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum in goats].
OBJECTIVE
To observe the protective immunity in goats induced by active immunization with anti-idiotypic monoclonal antibody NP30 of S. japonicum.


METHODS
Goats were randomly divided into two groups. Experimental group had 12 goats. The NP30 was intramuscularly injected with a dosage of 1 000 microgram into the hind leg for three dosages continuously. The goats were challenged with cercariae eight weeks after the third immunization. Ten goats in the control group were intramuscularly injected with SP2/0 ascites and challenged with cercariae at the same time as the experimental group. All goats were killed at the twelfth week post-challenge.


RESULTS
Active immunization with NP30 could produce a protection rate of 42.78% in goats. The number of eggs in liver and feces in the goats immunized with NP30 was decreased by 35.83% and 25%, respectively. The size and number of egg granulomas in the liver were lessened obviously and the weight of the goats increased significantly.


CONCLUSIONS
Active immunization with NP30 could induce a good protection in goats against the infection with Schsitosoma cercariae.
Feng
Z
Z
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.
Li
Y
Y
Qiu
Z
Z
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000909:Antibodies, Helminth / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D006041:Goats; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D010063:Ovum; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal