title: HPLC analysis of ecdysteroids in plant extracts using superheated deuterium oxide with multiple on-line spectroscopic analysis (UV, IR, 1H NMR, and MS).
abstract: HPLC, using superheated D20 as the mobile phase, combined with on-line characterization via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and mass spectrometry has been used for the analysis of a standard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometers enabled the on-flow collection of UV, 1H NMR, IR, and mass spectra not only for pure 20-hydroxyecdysone (100-400 microg on column) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
authors.affiliation: LGC, Runcom, Cheshire, UK.
authors.forename: Dave
authors.initials: D
authors.lastname: Louden
authors.forename: Alan
authors.initials: A
authors.lastname: Handley
authors.forename: Rene
authors.initials: R
authors.lastname: Lafont
authors.forename: Steve
authors.initials: S
authors.lastname: Taylor
authors.forename: Ian
authors.initials: I
authors.lastname: Sinclair
authors.forename: Eva
authors.initials: E
authors.lastname: Lenz
authors.forename: Timothy
authors.initials: T
authors.lastname: Orton
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D017666:Deuterium Oxide; D026461:Ecdysteroids / Q000032:analysis*; D004867:Equipment Design; D006358:Hot Temperature; D010936:Plant Extracts / Q000032:analysis*; D013057:Spectrum Analysis
chemical_list: D026461:Ecdysteroids; D010936:Plant Extracts; D017666:Deuterium Oxide