Gene induction during differentiation of human monocytes into dendritic cells: an integrated study at the RNA and protein levels.
Changes in gene expression occurring during differentiation of human monocytes into dendritic cells were studied at the RNA and protein levels. These studies showed the induction of several gene classes corresponding to various biological functions. These functions encompass antigen processing and presentation, cytoskeleton, cell signalling and signal transduction, but also an increase in mitochondrial function and in the protein synthesis machinery, including some, but not all, chaperones. These changes put in perspective the events occurring during this differentiation process. On a more technical point, it appears that the studies carried out at the RNA and protein levels are highly complementary.
Angénieux
C
C
EPI INSERM 9908, Biologie des cellules dendritiques, EFS Alsace, 10 rue Spielmann, 67065 Strasbourg, France.
Fricker
D
D
Strub
J M
JM
Luche
S
S
Bausinger
H
H
Cazenave
J P
JP
Van Dorsselaer
A
A
Hanau
D
D
de la Salle
H
H
Rabilloud
T
T
D002454:Cell Differentiation / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D003713:Dendritic Cells / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D009000:Monocytes / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000502:physiology; D012313:RNA / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D011506:Proteins; D020543:Proteome; D012313:RNA