title: [A study on antigen presentation of eosinophils in vivo and in vitro].
abstract: OBJECTIVE
  To elucidate the process and characteristics of antigen presentation of eosinophils in vitro and in vivo.
  
  
  METHODS
  BALB/c mice were sensitized and challenged by ovalbumin to recruit the aggregated eosinophil in the airways. The isolated airway eosinophils were co-cultured with sensitized T lymphocytes. Meanwhile, purified eosinophils were instilled into the trachea of sensitized mice, and then T cells from the draining lymph nodes were collected. The proliferation responses of T cells both in vitro and in vivo were observed.
  
  
  RESULTS
  In the absence of eosinophils in in vitro experiments, T cells did not proliferate even when incubated with exogenous antigen. The addition of ovalbumin sensitized eosinophils (in vivo) yielded significant eosinophil dose-dependent increases in T-cell proliferation. In sensitized mice that received airway instillation of antigen-exposed eosinophils, T-cell proliferation in the paratracheal lymph nodes developed within 1 day, reaching peak at day 3, and declined over 1 week. The in vivo T-cell proliferative responses increased with increasing numbers of eosinophils instilled into the tracheas. In both in vitro and in vivo experiments, antigen-exposed could only present antigen to T cells sensitized with the same antigen.
  
  
  CONCLUSIONS
  Eosinophils can uptake and process antigen both in vitro and in vivo, and then present processed antigen to sensitized T cells and thus induce T-cell proliferation. Moreover, eosinophil-induced T-cell proliferation is antigen specific.
authors.affiliation: First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
authors.forename: H
authors.initials: H
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004804:Eosinophils / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014132:Trachea / Q000166:cytology / Q000276:immunology