Biodegradation of anthracene and fluoranthene by fungi isolated from an experimental constructed wetland for wastewater treatment.
Pilot-scale constructed wetlands were used to treat water contaminated by polycyclic aromatic hydrocarbons (PAHs), particularly fluoranthene, and the possible role of fungi present in these ecosystems was investigated. A total of 40 fungal species (24 genera) were isolated and identified from samples (gravel and sediments) from a contaminated wetland and a control wetland. All of them were assayed for their ability to remove anthracene (AC) and fluoranthene (FA) from liquid medium. FA was degraded efficiently by 33 species while only 2 species were able to remove AC over 70%. A selection of 10 strains of micromycetes belonging to various taxonomic groups was further investigated for FA and AC degradation, toxicity assays and phenoloxidases (POx) detection. Interesting and not previously reported species were revealed (Absidia cylindrospora, Cladosporium sphaerospermum, and Ulocladium chartarum). They were all able to highly degrade the PAH-model compounds chosen. An interesting inducibility was noted for Ulocladium chartarum. Degradative ability of fungi was not related to their extracellular POx activity. This study may contribute to the improvement of constructed wetlands for water treatment, which may be enriched in efficient fungi.
Giraud
F
F
EA 2945, Environnement-Santé/GEDEXE, UFR de Pharmacie Grenoble, Université Joseph Fourier, Meylan, France.
Guiraud
P
P
Kadri
M
M
Blake
G
G
Steiman
R
R
D000873:Anthracenes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D017753:Ecosystem; D004791:Enzyme Inhibitors / Q000378:metabolism*; D005449:Fluorenes / Q000378:metabolism*; D005658:Fungi / Q000502:physiology*; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification
D000873:Anthracenes; D004791:Enzyme Inhibitors; D005449:Fluorenes; D014874:Water Pollutants, Chemical; C007738:fluoranthene; C034020:anthracene