Visualization of proteins in acrylamide gels using ultraviolet illumination.
Proteins in polyacrylamide gels can be rapidly visualized by soaking in trichloroacetic acid or chloroform followed by illumination with UV light. The UV-light-driven reaction of tryptophan in the presence of trichlorocompounds yields products that emit sufficiently in the visible region to identify the location of the protein bands on the gel. This method can be used to rapidly identify protein bands on a gel in less than 20 min. On thin polyacrylamide gels, 1.0 microg of protein can easily be detected for proteins with typical tryptophan percentages.
Kazmin
Dmitri
D
Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.
Edwards
Robert A
RA
Turner
Raymond J
RJ
Larson
Eric
E
Starkey
Jean
J
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D011506:Proteins / Q000032:analysis*; D014364:Tryptophan / Q000737:chemistry*; D014466:Ultraviolet Rays
D011506:Proteins; D014364:Tryptophan