title: Molecular genetic analysis of a small bowel primitive neuroectodermal tumor.
abstract: We present a pediatric peripheral primitive neuroectodermal tumor (pPNET) localized exclusively to the small bowel. The tumor presented in an adolescent male and the diagnosis was confirmed by electron microscopy, CD99 immunopositivity, and molecular genetic analysis that demonstrated an EWS-FLI1 type 2 fusion transcript. This case report and a review of the literature underscore the considerable phenotypic overlap in EWS-related tumors in this site and the necessity for molecular genetic analysis to permit accurate classification.
authors.affiliation: Department of Hematology, Oncology, and Bone Marrow Transplant, The Children's Hospital, University of Colorado Health Sciences Center, Denver, CO 80218, USA.
authors.forename: Douglas K
authors.initials: DK
authors.lastname: Graham
authors.forename: Linda C
authors.initials: LC
authors.lastname: Stork
authors.forename: Qi
authors.initials: Q
authors.lastname: Wei
authors.forename: J David
authors.initials: JD
authors.lastname: Ingram
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Karrer
authors.forename: Gary W
authors.initials: GW
authors.lastname: Mierau
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lovell
mesh_terms: D000293:Adolescent; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D018242:Neuroectodermal Tumors, Primitive / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012983:Soft Tissue Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D014057:Tomography, X-Ray Computed; D014157:Transcription Factors
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C111598:EWS-ATF1 fusion protein, human; D015514:Oncogene Proteins, Fusion; D014157:Transcription Factors