Crystallization and preliminary X-ray analysis of the membrane-bound cytochrome c nitrite reductase complex (NrfHA) from Wolinella succinogenes.
Crystals of the complex between the enzyme cytochrome c nitrite reductase (NrfA) and the membrane-bound quinol oxidase and electron carrier NrfH were grown by vapour diffusion using ammonium sulfate as a precipitant. In the epsilon-proteobacterium Wolinella succinogenes, NrfA and NrfH form a functional membrane-bound complex which catalyzes the last step in the metabolic pathway of nitrate dissimilation. NrfH represents a prototype of a large family of putative bacterial quinol oxidases, the NapC/NirT family, which have been proposed to serve as electron donors for a variety of reductases. Crystal growth of the NrfHA complex was strongly dependent on the presence of detergent; the crystals grown belonged to space group I422.
Einsle
Oliver
O
Max-Planck-Institut für Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, 82152 Martinsried, Germany.
Stach
Petra
P
Messerschmidt
Albrecht
A
Klimmek
Oliver
O
Simon
Jörg
J
Kröger
Achim
A
Kroneck
Peter M H
PM
D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D045263:Cytochromes a1; D003575:Cytochromes c1; D008958:Models, Molecular; D009565:Nitrate Reductases / Q000737:chemistry* / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D016601:RNA-Binding Proteins; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D016968:Wolinella / Q000201:enzymology*
D001426:Bacterial Proteins; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C087372:nfrA protein, Azorhizobium caulinodans; D045263:Cytochromes a1; D003575:Cytochromes c1; D010088:Oxidoreductases; C020094:duroquinol oxidase; D009565:Nitrate Reductases; C054690:nitrate reductase (cytochrome)