Differential effect of jasmonic acid and abscisic acid on cell cycle progression in tobacco BY-2 cells.
Environmental stress affects plant growth and development. Several plant hormones, such as salicylic acid, abscisic acid (ABA), jasmonic acid (JA), and ethylene play a crucial role in altering plant morphology in response to stress. Developmental regulation often has the cell cycle machinery among its targets. We analyzed the effect of JA and ABA on cell cycle progression in synchronized tobacco (Nicotiana tabacum) BY-2 cells. Both compounds were found to prevent DNA replication, keeping the cells in the G1 stage, when applied just before the G1/S transition. However, ABA did not have any effect on subsequent phases of the cell cycle when applied at a later stage, whereas JA effectively prevented mitosis on application during DNA synthesis. This demonstrates that JA treatment can freeze synchronized BY-2 cells in both the G1 and G2 stages of the cell cycle. Jasmonate administered after the S-phase was less effective in decreasing the mitotic index, suggesting that cell sensitivity toward JA is dependent on the cell cycle phase. In cultures detained in the G2-phase, we observed a reduced histone H1 kinase activity of kinases associated with the p13(suc1) protein.
Swiatek
Agnieszka
A
Laboratory of Plant Physiology and Biochemistry, Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
Lenjou
Marc
M
Van Bockstaele
Dirk
D
Inzé
Dirk
D
Van Onckelen
Harry
H
D000040:Abscisic Acid / Q000494:pharmacology*; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000166:cytology / Q000254:growth & development*
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D000040:Abscisic Acid; D011494:Protein Kinases; C102333:histone H1 kinase