[Effects of the anti-LCAMs monoclonal antibodies on proliferation of pulmonary fibrosis in vitro].
OBJECTIVE
To explore the effects of the antibodies to LCAMs on proliferation of pulmonary fibroblast (L929 cell line).


METHODS
Proliferation of fibroblasto and level of collagen production were determined by (3)H-TDR incorporation and ELISA.


RESULTS
The supernate of AMs cultured with anti-LCAMs could inhibit the proliferation of fibroblast. The presence of anti-CD(11)B (125 microg/ml) could significantly inhibit the proliferation of fibroblast (P < 0.05), and reduce collagen secretion by 29.7%. When anti-CD(18) and anti-CD(54) at the concentration of 25 microg/ml, 5 microg/ml respectively, the inhibition rate of fibroblast proliferation differed from that in control group (P < 0.05). moreover combination of the two antibodies resulted in more significant inhibition effect than that seen by the two antibodies separately.


CONCLUSIONS
The increased expression of CAMs on AM might play an important role in the pathogenesis of the pulmonary fibrosis and MAbs to CD(11)B/CD(18), CD(54) possess a potential regiment for intervention of pulmonary fibrosis.
Tong
Z
Z
Respiratory Disease Institute, China Medical University, Shenyang 110001.
Zhao
Y
Y
Zhao
H
H
Hou
X
X
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D039481:CD11b Antigen / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D002455:Cell Division; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008297:Male; D051379:Mice; D011658:Pulmonary Fibrosis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; D018821:CD18 Antigens; D018799:Intercellular Adhesion Molecule-1; D003094:Collagen