VLA-2 and VLA-5 cell adhesion molecules expression in CD34+ cells from umbilical cord blood and from bone marrow.
OBJECTIVE
Umbilical cord blood contains a large number of early hematopoietic cells with high proliferating capacity, that has been used as an alternative to bone marrow transplantation. The aim of this study is to investigate the number of two cell adhesion molecules in cord blood and in bone marrow.


METHODS
We investigated two integrins, named VLA-2 and VLA-5 (Very Late Appearing Antigen), expressed in the surface of CD34+ cells. The CD34+ cells, isolated with MACS CD34+ isolation kit, were labelled with the appropriate monoclonal antibodies.


RESULTS
Cell adhesion molecules showed highly expressed in both cord blood and bone marrow CD34+ cells.


CONCLUSIONS
There are no significant differences between the two sources of CD34+ populations.
Pafumi
C
C
Department of Microbiological and Gynaecological Sciences, University of Catania, Italy. pafumi@mbox.unict.it
Mancari
R
R
Parisi
G
G
Farina
M
M
Russo
A
A
Pernicone
G
G
Bandiera
S
S
Giardina
P
P
Mirisola
T
T
Stagno
F
F
Calogero
A E
AE
D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002469:Cell Separation; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007118:Immunoassay; D038982:Integrin alpha2beta1 / Q000032:analysis*; D039081:Integrin alpha5beta1 / Q000032:analysis*
D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D038982:Integrin alpha2beta1; D039081:Integrin alpha5beta1