Identification of a dopamine receptor from Caenorhabditis elegans.
The neurotransmitter dopamine regulates locomotion and egg laying in the nematode Caenorhabditis elegans. We have cloned a cDNA encoding the C. elegans G protein-coupled receptor (CeDOP1). The deduced amino acid sequence of the cloned cDNA shows high sequence similarities with D1-like dopamine receptors from other species. Three splice variants that differ in the length of the predicted third intracellular loop and C-terminal tail were identified. COS-7 cells transiently transfected with CeDOP1 showed high affinity binding to [(125)I]iodo-lysergic acid diethylamide (K(D)=3.43 +/- 0.83 nM). Dopamine showed the highest affinity (K(i)=0.186 microM) for this receptor among several vertebrate and invertebrate amine neurotransmitters tested, suggesting that the natural ligand for this receptor is dopamine.
Suo
Satoshi
S
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Sasagawa
Noboru
N
Ishiura
Shoichi
S
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001667:Binding, Competitive / Q000187:drug effects / Q000502:physiology; D019556:COS Cells; D017173:Caenorhabditis elegans / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004298:Dopamine / Q000378:metabolism*; D005247:Feeding Behavior / Q000502:physiology; D005786:Gene Expression Regulation / Q000235:genetics; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008024:Ligands; D008238:Lysergic Acid Diethylamide / Q000493:pharmacokinetics; D008969:Molecular Sequence Data; D009420:Nervous System / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012702:Serotonin Antagonists / Q000493:pharmacokinetics; D014162:Transfection
D018076:DNA, Complementary; D007457:Iodine Radioisotopes; D008024:Ligands; D017447:Receptors, Dopamine D1; D012702:Serotonin Antagonists; D008238:Lysergic Acid Diethylamide; D004298:Dopamine