Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
During prophase in higher cells, centrosomes localize to deep invaginations in the nuclear envelope in a microtubule-dependent process. Loss of nuclear membranes in prometaphase commences in regions of the nuclear envelope that lie outside of these invaginations. Dynein and dynactin complex components concentrate on the nuclear envelope prior to any changes in nuclear envelope organization. These observations suggest a model in which dynein facilitates nuclear envelope breakdown by pulling nuclear membranes and associated proteins poleward along astral microtubules leading to nuclear membrane detachment. Support for this model is provided by the finding that interference with dynein function drastically alters nuclear membrane dynamics in prophase and prometaphase.
Salina
Davide
D
Department of Cell Biology and Anatomy, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.
Bodoor
Khaldon
K
Eckley
D Mark
DM
Schroer
Trina A
TA
Rattner
J B
JB
Burke
Brian
B
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D016466:CHO Cells; D018385:Centrosome / Q000378:metabolism; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D000072159:Dynactin Complex; D004398:Dyneins / Q000378:metabolism*; D016195:G2 Phase / Q000502:physiology; D007668:Kidney / Q000166:cytology; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D011418:Prophase / Q000502:physiology; D051381:Rats
D000970:Antineoplastic Agents; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D004398:Dyneins; D015739:Nocodazole