title: Evaluation of various solutions for small bowel graft preservation.
abstract: AIM:To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage.METHODS:The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer's (LR), Euro-Collins (EC), hyperosmolarity citrate adenine (HC-A) and WMO-1 solutions for 10,18 and 24 hours,respectively.The recipient survival rate, morphological structure, graft mucosal energy substances and Na( +) -K(+) ATPase activity were studied,and graft absorption was estimated with D-xylose absorption test.RESULTS:The morphological study of the grafts preserved with LR or HC-A solution for 10 hours or with EC and WMO-1 solution for 18 hours was normal 6days after operation. Mucosal ATP,total adenine nucleotides (TAN) contents and Na( +) -K(+)ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC-A solution.Serum level of D-xylose was higher in EC and WMO-1 groups than in LR and HC-A groups when the graft was preserved for 24 hours.CONCLUSION:EC and WMO-1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC-A solutions.
authors.forename: You-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Jie-Shou
authors.initials: JS
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Jiang
authors.forename: Yun-Zhao
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Nan-Yun
authors.initials: NY
authors.lastname: Li
authors.forename: Fang-Nan
authors.initials: FN
authors.lastname: Liu