Qualitative thin-layer and high-performance liquid chromatographic analysis of 1-substituted diazen-1-ium-1,2-diolates on aminopropyl-bonded silica gel.
High-performance liquid (HPLC) and thin-layer (TLC) chromatographic methods for the detection and quantification of diazeniumdiolates are described. The HPLC determinations were made using a Rocket Platinum NH2 column (7 x 53 mm, 100-A pore size, 3-microm particle size), under isocratic conditions with mixtures of acetonitrile, methanol, and water containing 0.1% diethylamine (v/v), at a flow rate of 2.5 ml/min, a column temperature of 22 degrees C, and UV detection at 250 nm. The TLC determinations were similarly made using Merck NH2 F254S precoated glass plates (approximately 2 x 5 cm, 5-microm particle size, 0.2-mm layer thickness) with mixtures of acetonitrile, methanol, and water containing 0.1% diethylamine (v/v). Preexisting traces of carcinogenic nitrosamines were detected in some samples of diazeniumdiolates. The methods provide a more efficient means of characterizing the purity of diazeniumdiolate samples prepared for use in biomedical applications than older procedures which rely on differential absorbance measurements at 250 and 350 nm, respectively.
Fitzhugh
Anthony L
AL
Intramural Research Support Program, SAIC Frederick, National Cancer Institute at Frederick, Maryland 21702-1201, USA. afitzhugh@mail.ncifcrf.gov
Anadu
Nwanne O
NO
Waterhouse
David J
DJ
Hrabie
Joseph A
JA
Saavedra
Joseph E
JE
Keefer
Larry K
LK
D001391:Azo Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D009602:Nitrosamines / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry
D001391:Azo Compounds; D009602:Nitrosamines; C021611:aminopropyl silica gel; C452539:diazeniumdiolate; D012822:Silicon Dioxide