Multilocus hybridization typing in Pediococcus acidilactici strains.
In previous a study we demonstrated the presence of several genomic subpopulations within a collection of Pediococcus acidilactici strains isolated from different environments, through a multilocus typing analysis taking into consideration housekeeping conserved loci and protein coding genes of the primary metabolism. In this study, representative strains of five genomic subpopulations previously described (I, II, III, V, VII) were analyzed by restriction analysis of chromosomal DNA and subsequent hybridization assays using as probes amplified fragments obtained from five housekeeping genes (16S rDNA, rpoC, ldhD, ldhL, and metS). A computer similarity and clustering analysis of hybridization data showed the subdivision of P. acidilactici strains in five distinct genotypes according to the grouping previously obtained confirming that pediocin AcH/PA-1 producer strains represent one genomic lineage within the species P. acidilactici.
Mora
Diego
D
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy. diego.mora@unimi.it
Parini
Carlo
C
Fortina
Maria Grazia
MG
Manachini
Pier Luigi
PL
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000737:chemistry / Q000235:genetics; D016000:Cluster Analysis; D015342:DNA Probes; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010376:Pediococcus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000737:chemistry / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; D012337:RNA, Ribosomal, 18S; D007770:L-Lactate Dehydrogenase; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; C108570:beta' subunit of RNA polymerase; D012321:DNA-Directed RNA Polymerases