[The diagnostic significance of antineutrophil cytoplasmic antibodies in ulcerative colitis].
OBJECTIVE
To inquire into the diagnostic significance of antineutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis (UC).


METHODS
Serum ANCA from 58 UC patients, 43 non-UC patients and 58 healthy donors were detected with indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA)and Western blot.


RESULTS
The sensitivity and specificity of the presence of ANCA in the diagnosis of UC were 37.93% and 100% respectively. In the group of UC, patients with mild, moderate and severe clinical manifestations had positive ANCA rates of 17.65%, 41.67% and 52.94% respectively. The occurrence rates of mucosal vasculitis and grade III-V mucosal inflammation were 78.95% and 78.95% respectively in ANCA-positive group, but 37.04% and 44.44% in ANCA-negative group. The levels of the binding of the five kinds of ANCA antigens i.e. myeloperoxidase (MPO), bactericidal/permeability increasing protein (BPI), lactoferrin (LF), cathepsin G (CG) and proteinase-3 (PR-3) with the serum of UC patients were 13.99%, 13.79%, 10.34% 10.34% and 8.62% respectively. Specific protein strips were observed in 48.28% of the UC patients with Western blot technique, 47,000 polypeptide being the most common and making up 22.41% of them.


CONCLUSIONS
The detection of ANCA was an auxiliary method for the diagnosis of UC. At present, UC-associated target antigens were not yet clarified, 47,000 polypeptide may be one of them. ANCA may participate in the pathogenesis of UC.
Liu
X
X
Department of Internal Medicine, First Teaching Hospital of Beijing Medical University, Beijing 100034.
Yu
T
T
Zhao
M
M
Tang
X
X
Gu
Q
Q
Liu
N
N
D000293:Adolescent; D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity
D019268:Antibodies, Antineutrophil Cytoplasmic