title: The synthesis and fate of glycodelin in human ovary during folliculogenesis.
abstract: The ontogeny of glycodelin in human ovarian follicles during folliculogenesis was studied. Glycodelin immunoreactivity began to be detected in the granulosa cells and thecal cells of late secondary follicles. Immunoreactivity was also found in both the luteinized granulosa cells and cumulus cells obtained from women undergoing the assisted reproduction treatment. However, only the luteinized granulosa cells, and not the cumulus cells, expressed glycodelin mRNA. Results also showed that the cumulus cells took up radiolabelled glycodelin and partially deglycosylated some of it. Glycodelin (and a partially deglycolsylated form of glycoldelin) appeared to complex with two cytoplasmic or membrane components of the cumulus cells. The data also demonstrated that ZIF-1, a glycoprotein isolated from human follicular fluid, was immunologically similar to glycodelin. In conclusion, we suggest that glycodelin is synthesized in the granulosa cells of ovarian follicles at late secondary follicle stage. It then may be released into the follicular fluid from where it is taken up and partially modified by the cumulus cells.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Queen Mary Hospital, Hong Kong, P.R.China.
authors.forename: J Y M
authors.initials: JY
authors.lastname: Tse
authors.forename: P C N
authors.initials: PC
authors.lastname: Chiu
authors.forename: K F
authors.initials: KF
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Seppala
authors.forename: H
authors.initials: H
authors.lastname: Koistinen
authors.forename: R
authors.initials: R
authors.lastname: Koistinen
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Yao
authors.forename: W S B
authors.initials: WS
authors.lastname: Yeung
mesh_terms: D001667:Binding, Competitive; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism*; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger; D013799:Theca Cells / Q000378:metabolism
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; D007457:Iodine Radioisotopes; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D012333:RNA, Messenger