Genetic evidence that the uvsE gene product of Deinococcus radiodurans R1 is a UV damage endonuclease.
An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruption of dr1819 greatly sensitizes the uvrA-1 strain but not the uvrA-1(+) strain to UV light, indicating that the dr1819 gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of dr1819 will restore UV resistance to UVS78, a uvrA-1 uvsE strain, indicating that dr1819 and uvsE are the same locus.
Earl
Ashlee M
AM
Department of Biological Sciences, Louisiana State University and A & M College, Baton Rouge, Louisiana 70803, USA.
Rankin
Sara K
SK
Kim
Kwang-Pyo
KP
Lamendola
Oleana N
ON
Battista
John R
JR
D001426:Bacterial Proteins; D045643:DNA Repair Enzymes; D004706:Endodeoxyribonucleases / Q000235:genetics*; D005798:Genes, Bacterial; D013824:Thermus / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D019895:Transposases; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D019895:Transposases; D004706:Endodeoxyribonucleases; C055508:UvsD protein, Deinococcus radiodurans; D045643:DNA Repair Enzymes