title: CD64 surface expression on neutrophils is transiently upregulated in patients with septic shock.
abstract: OBJECTIVE
  To clarify the changes in total leukocyte counts, CD64 neutrophil receptor expression and serum granulocyte colony-stimulating factor (G-CSF) concentrations in critically ill patients without infection and sepsis and in patients with septic shock.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Intensive care unit (ICU) and research laboratory of a university hospital.
  
  
  METHODS
  Eleven critically ill patients without infections and 22 patients with proven infections in septic shock for the first time and of at least 3 days' duration.
  
  
  RESULTS
  Over a 6month period, a longitudinal analysis of expression of the monomeric Fc receptor type I (CD64, FcgammaRI) on neutrophils was performed by flow cytometric analysis on a daily basis in all postoperative/post-traumatic patients admitted to the ICU until discharge from the ICU or death. Out of 273 patients, 11 patients without sepsis had organ failure and 22 patients with proven infections had septic shock for the first time and of at least 3 days' duration. Ten out of the 22 patients survived, 12 died. CD64 expression was greater in patients with septic shock than in patients without sepsis. Moreover, CD64 expression was only initially and transiently elevated in most survivors (9/10) and non-survivors (8/12) of septic shock. In survivors, G-CSF serum concentrations were markedly decreased in the 2nd week.
  
  
  CONCLUSIONS
  Decreased neutrophil CD64 expression in an acutely ill population with septic shock may reflect the development of a non-responsive state as well as the early downregulation of neutrophil activation prior to the resolution of an ongoing infection.
authors.affiliation: Universitaetsklinik für Anaesthesiologie, Universitaetsklinikum für Anaesthesiologie, Steinhoevelstrasse 9, 89075 Ulm, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Fischer
authors.forename: E M
authors.initials: EM
authors.lastname: Schneider
authors.forename: L L
authors.initials: LL
authors.lastname: L  Moldawer
authors.forename: C
authors.initials: C
authors.lastname: Karcher
authors.forename: E
authors.initials: E
authors.lastname: Barth
authors.forename: H
authors.initials: H
authors.lastname: Suger-Wiedeck
authors.forename: M
authors.initials: M
authors.lastname: Georgieff
authors.forename: M
authors.initials: M
authors.lastname: Weiss
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D005858:Germany / Q000453:epidemiology; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D011446:Prospective Studies; D017452:Receptors, IgG / Q000378:metabolism*; D012772:Shock, Septic / Q000276:immunology* / Q000401:mortality; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D015854:Up-Regulation
chemical_list: D017452:Receptors, IgG; D016179:Granulocyte Colony-Stimulating Factor