Development of a chromatographic method for the determination of saquinavir in plasma samples of HIV patients.
A simple, sensitive and reproducible high-performance liquid chromatographic method for detecting and quantifying saquinavir in human plasma is described. Verapamil was used as internal standard. The method employes a single liquid-liquid extraction step with tert-butil methyl ether followed by chromatography on a Lichrospher 60 Select B C8 reversed-phase column. Ultraviolet detection was used to identify the compounds of interest. The quantitation limit of saquinavir was 1 ng/mL and only 0.5 mL of plasma sample was required for the determination. The average saquinavir recoveries over a concentration range of 2.5-500 ng/mL ranged from 86 to 95%. Precision and accuracy did not exceed 5%.
Campanero
M A
MA
Department of Clinical Pharmacology, Clinica Universitaria de Navarra, Pamplona, Spain. macampaner@unav.es
Escolar
M
M
Arangoa
M A
MA
Sádaba
B
B
Azanza
J R
JR
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D019258:Saquinavir / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D017320:HIV Protease Inhibitors; D019258:Saquinavir