title: Study of ethidium bromide interaction peculiarities with DNA.
abstract: The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M < or = muNa+ < or = 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex--(deltaT) becomes equal to that of DNA itself (deltaTo). It has been shown that the values of r(b) at which delta deltaT=deltaT-deltaT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.
authors.affiliation: Biophysics Department of the Biological Faculty of Yerevan State University, Armenia. kensafiz@ysu.am
authors.forename: P O
authors.initials: PO
authors.lastname: Vardevanyan
authors.forename: A P
authors.initials: AP
authors.lastname: Antonyan
authors.forename: G A
authors.initials: GA
authors.lastname: Manukyan
authors.forename: A T
authors.initials: AT
authors.lastname: Karapetyan
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D006358:Hot Temperature; D008024:Ligands; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D012996:Solutions
chemical_list: D008024:Ligands; D012996:Solutions; D004247:DNA; D004996:Ethidium