[Calcium-binding protein S100A2 in the retardation of growth and proliferation of hepatocellular carcinoma cells].
OBJECTIVE
To study the retardation effect of calcium-binding protein S100A2 on the growth and proliferation of hepatocellular carcinoma QGY7701 cells.


METHODS
After the plasmid of EGFP-S100A2 had been regrouped and introduced into the hepatocellular carcinoma QGY7701 cells with lipofectin, the expression and location of the products were observed by fluorescent microscopy. The cell growth and proliferation were monitored through cell colony formation in vitro and xenografting subcutaneously in the nude mice in vivo. The effect of S100A2 on the QGY7701 cell cycle was examined with flow cytometry.


RESULTS
The chimera protein of S100A2 and green fluorescent protein were detected and appeared to be localized in the cytoplasm and nucleus, while the green fluorescent protein was found to be localized only in the cytoplasm. The cell colony formation of QGY7701/A2 was significantly reduced as compared with the controls. The xenografted tumor of QGY7701/A2 in the nude mice showed a growth at a considerably slower rate than that of QGY7701/pc and QGY7701 groups. The cell cycle review showed retardation of QGY7701/A2 cells in the G1 phase and the DNA content was obviously reduced as compared with the controls.


CONCLUSIONS
The exogenous S100A2 is able to check the QGY7701 cell cycle, stop the cell growth and proliferation either in vitro or in vivo in the QGY7701 cells.
Wang
X
X
Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Chen
L
L
Zeng
Y
Y
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology*; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002630:Chemotactic Factors / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use; D009418:S100 Proteins / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D002630:Chemotactic Factors; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D009418:S100 Proteins; C098997:S100A2 protein, human; D049452:Green Fluorescent Proteins