title: Sterol and fatty acid composition of Candida lusitaniae clinical isolates.
abstract: The sterol and fatty acid compositions of four amphotericin B-resistant and of two amphotericin B-susceptible Candida lusitaniae clinical isolates were determined. A flow cytofluorometric susceptibility test (FCST) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. Compared to susceptible isolates, resistant ones showed a greatly reduced ergosterol content and changes in sterol composition, consistent with a defect in Delta8-->7 isomerase. Within each group, no correlation between the sterol or fatty acid pattern or composition and both the degree of in vitro susceptibility and FCST MIC was found.
authors.affiliation: Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire 13385 Marseille, France.
authors.forename: F
authors.initials: F
authors.lastname: Peyron
authors.forename: A
authors.initials: A
authors.lastname: Favel
authors.forename: R
authors.initials: R
authors.lastname: Calaf
authors.forename: A
authors.initials: A
authors.lastname: Michel-Nguyen
authors.forename: R
authors.initials: R
authors.lastname: Bonaly
authors.forename: J
authors.initials: J
authors.lastname: Coulon
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry*; D006801:Humans; D013261:Sterols / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D005227:Fatty Acids; D013261:Sterols; D000666:Amphotericin B