title: Oxygen transfer rate and sophorose lipid production by Candida bombicola.
abstract: Sophorose lipids (SLs) have applications as surfactants and are produced at high levels by several yeasts. We developed a fed-batch shake-flask method for the production of SLs by Candida bombicola ATCC 22214. Optimal aeration, expressed in terms of oxygen transfer rate, was between 50 and 80 mM O(2)/L h(-1) and resulted in maximum values for both volumetric product formation (1-1.5 g/L h(-1)) and SL yield (350 g/L). The lowest aeration levels resulted in the enrichment in saturated fatty acid SLs at the expense of unsaturated fatty acid SLs.
authors.affiliation: Department of Chemistry, Chemical Engineering, and Material Science, Polytechnic University, Brooklyn, NY 11201, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Guilmanov
authors.forename: Alberto
authors.initials: A
authors.lastname: Ballistreri
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Impallomeni
authors.forename: Richard A
authors.initials: RA
authors.lastname: Gross
mesh_terms: D001709:Biotechnology; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005285:Fermentation; D005936:Glucans / Q000378:metabolism*; D006017:Glycolipids / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005936:Glucans; D006017:Glycolipids; C067958:16-(2'-O-glucopyranosylglucopyranosyloxy)hexadecanoic acid 1',4''-lactone 6',6''-diacetate; D010100:Oxygen; C003328:sophorose