title: Evidence for facilitated lactate uptake in lizard skeletal muscle.
abstract: To understand more fully lactate metabolism in reptilian muscle, lactate uptake in lizard skeletal muscle was measured and its similarities to the monocarboxylate transport system found in mammals were examined. At 2 min, uptake rates of 15 mmol l(-1) lactate into red iliofibularis (rIF) were 2.4- and 2.2-fold greater than white iliofibularis (wIF) and mouse soleus, respectively. alpha-Cyano-4-hydroxycinnamate (15 mmol l(-1)) caused little inhibition of uptake in wIF but caused a 42-54 % reduction in the uptake rate of lactate into rIF, suggesting that much of the lactate uptake by rIF is via protein-mediated transport. N-ethymaleimide (ETH) (10 mmol l(-1)) also caused a reduction in the rate of uptake, but measurements of adenylate and phosphocreatine concentrations show that ETH had serious effects on rIF and wIF and may not be appropriate for transport inhibition studies in reptiles. The higher net uptake rate by rIF than by wIF agrees with the fact that rIF shows much higher rates of lactate utilization and incorporation into glycogen than wIF. This study also suggests that lactate uptake by reptilian muscle is similar to that by mammalian muscle and that, evolutionarily, this transport system may be relatively conserved even in animals with very different patterns of lactate metabolism.
authors.affiliation: Section of Integrative Physiology and Neurobiology, E.P.O. Biology, University of Colorado-Boulder, Boulder, CO 80309-0334, USA. donovane@colorado.edu
authors.forename: E R
authors.initials: ER
authors.lastname: Donovan
authors.forename: T T
authors.initials: TT
authors.lastname: Gleeson
mesh_terms: D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D007076:Iguanas / Q000378:metabolism*; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism*; D051379:Mice; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D005033:Ethylmaleimide