title: The protective efficacy of a recombinant VP2-based African horsesickness subunit vaccine candidate is determined by adjuvant.
abstract: We previously demonstrated that soluble baculovirus-expressed African horsesickness virus (AHSV) serotype 5 VP2 protein (AHSV5 rVP2) elicits neutralising antibodies in guinea pigs. We have now determined the immunogenicity of soluble AHSV5 rVP2 in horses when administered in three different adjuvant types, ISA-50, aluminium phosphate and different saponin preparations. Doses of 10 and 50microg of rVP2 administered with saponin induced full protection to a lethal challenge, albeit with dose-related side effects. The results establish that soluble rVP2 is the biologically active form and that it can induce complete protection when it is delivered with saponin adjuvants. We conclude that the use of the soluble biologically active form of AHSV rVP2 and the choice of adjuvant will be crucial factors in determining efficacy, safety and the production cost of recombinant AHSV subunit vaccines.
authors.affiliation: Onderstepoort Veterinary Institute, 0110, Onderstepoort, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Scanlen
authors.forename: J T
authors.initials: JT
authors.lastname: Paweska
authors.forename: J A
authors.initials: JA
authors.lastname: Verschoor
authors.forename: A A
authors.initials: AA
authors.lastname: van Dijk
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000356:African Horse Sickness Virus / Q000276:immunology*; D000818:Animals; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D006736:Horses; D007114:Immunization; D012503:Saponins / Q000009:adverse effects / Q000494:pharmacology; D022223:Vaccines, Subunit / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D036022:Capsid Proteins; D012503:Saponins; C442519:VP2 protein, African horse sickness virus; D022223:Vaccines, Subunit; D014614:Vaccines, Synthetic; D014765:Viral Vaccines