Bone aluminum uptake in uremic rats receiving intraperitoneal iron.
To assess the effect of concomitant iron and aluminum loads on bone aluminum accumulation and on the response to the deferoxamine test in rats with the same aluminum surcharge, Wistar rats with chronic renal failure were divided into three groups: iron-overloaded rats (N = 6) (intraperitoneal iron); iron-depleted rats (N = 6) (blood withdrawal two to three times per week); control rats (N = 4) (no manipulation). All groups received intraperitoneal aluminum simultaneously. After 6 wk, a deferoxamine challenge test was performed. Thereafter, bone aluminum and iron were measured. The iron-overloaded rats showed higher bone iron content (iron overloaded: 147.7+/-55.4 microg/g; iron depleted: 7.9+/-1.0, and controls 13.3+/-9.9 microg/g, p < 0.010) and lower bone aluminum content (iron overloaded: 14.2+/-4.0 microg/g; iron depleted: 70.9+/-35.1 microg/g; controls: 72.7+/-28.3 microg/g p < 0.005). No differences were found between the iron-depleted and control rats. After the deferoxamine infusion, the iron-depleted rats tended to have higher serum aluminum increments (p = NS) and higher urinary aluminum excretion (p < 0.012, p < 0.020) than control rats despite similar amounts of aluminum in bone of the two groups. Aluminum bone accumulation was minor if iron and aluminum loads were given concomitantly. The iron depletion influenced the results of the deferoxamine challenge test in rats with similar bone aluminum burden.
Fernández-Martín
J L
JL
Bone and Mineral Research Unit, Instituto Reina Sofía de Investigación, Hospital Central de Asturias, Oviedo, Spain.
Fernández-Soto
I
I
Acuña
G
G
Cannata-Andía
J B
JB
D000535:Aluminum / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D003676:Deferoxamine / Q000378:metabolism; D004921:Erythropoietin / Q000378:metabolism; D007501:Iron / Q000378:metabolism* / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000378:metabolism; D014511:Uremia / Q000378:metabolism*
D004921:Erythropoietin; D000535:Aluminum; D007501:Iron; D003676:Deferoxamine