title: DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III.
abstract: The temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity.
authors.affiliation: MRC Cell Mutation Unit, University of Sussex, Falmer, BN1 9RR, Brighton, UK
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Timms
authors.forename: Bryn A
authors.initials: BA
authors.lastname: Bridges
mesh_terms: D004258:DNA Polymerase III / Q000235:genetics* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D009154:Mutation; D013014:SOS Response, Genetics; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; C078202:UmuC protein, E coli; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III; C120926:DNA polymerase V, E coli; D004259:DNA-Directed DNA Polymerase; C055218:UmuD protein, E coli; C468316:dnaQ protein, E coli; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V