Characterization of a cytosolic estrogen receptor and its up-regulation by 17 beta-estradiol and the xenoestrogen 4-tert-octylphenol in the liver of eelpout (Zoarces viviparus).
Estrogen binding activity was revealed in the cytosolic fraction of hepatic extracts from adult male and female eelpout (Zoarces viviparus). The binding moiety was characterized by a single class of high affinity binding sites (K(d)=0.59+/-0.05 nM in males and 1.06+/-0.10 nM in females). The affinity was significantly higher in males. Binding sites were satiable and binding capacity was significantly elevated in vitellogenic females (2.92+/-0.28 pmol/g) compared to males (1.67+/-0.11 pmol/g). The binding was specific to known estrogens but not to other tested steroids. The binding moiety was able to bind to DNA-cellulose and was extractable by high salt concentrations. A time-course study of estrogen binding activity in liver cytosol and of vitellogenin (Vtg) in plasma, after intraperitoneal (i.p.) injections of 17 beta-estradiol (E(2)) in male eelpout, was carried out. It was shown that both are inducible by E(2). Estrogen binding activity was significantly elevated 48 h and Vtg 72 h after E(2) treatment. The binding moiety was hereafter designated as a cytosolic estrogen receptor (ER). The estrogenicity of 4-tert-octylphenol (OP) was evaluated by measuring ER and Vtg after i.p. treatment. OP-treatment increased both receptor levels and Vtg concentrations in male fish, indicating that OP acts as an estrogen in male eelpout.
Andreassen
T K
TK
Institute of Biology, University of Southern Denmark, Odense University, Campusvej 55, DK-5230 Odense, Denmark.
Korsgaard
B
B
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D004967:Estrogens / Q000032:analysis / Q000378:metabolism; D005260:Female; D005399:Fishes / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D010636:Phenols / Q000633:toxicity*; D011485:Protein Binding; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D012044:Regression Analysis; D012621:Seasons; D013501:Surface-Active Agents / Q000633:toxicity; D015854:Up-Regulation; D014819:Vitellogenins / Q000097:blood / Q000187:drug effects
C033190:DNA-cellulose; D004967:Estrogens; D010636:Phenols; D011960:Receptors, Estrogen; D013501:Surface-Active Agents; D014819:Vitellogenins; D004958:Estradiol; D002482:Cellulose; D004247:DNA; C105260:4-tert-octylphenol