The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru.
A 3599 nucleotide portion of the genomic RNA of a UK isolate of Pepino mosaic virus (PepMV), isolated from tomato, has been sequenced (Accession No. AF340024). The region sequenced includes the 3'-end of the RNA polymerase, the triple gene block (TGB), the coat protein (CP) and 3' untranslated region (UTR). In addition, the CP sequences of another 15 PepMV isolates, including 14 European tomato isolates and a Peruvian pepino isolate, have been determined and compared. This analysis shows that all the tomato isolates share over 99% identity, but only between 96-97% identity with the Peruvian pepino isolate.
Mumford
R A
RA
Central Science Laboratory, Sand Hutton, York, UK. r.mumford@csl.gov.uk
Metcalfe
E J
EJ
D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics*; D005060:Europe; D016679:Genome, Viral; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010568:Peru; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D006113:United Kingdom
D012367:RNA, Viral