title: Role of P-selectin and anti-P-selectin monoclonal antibody in apoptosis during hepatic/renal ischemia reperfusion injury.
abstract: AIM:To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during hepatic/renal ischemia-reperfusion injury.METHODS:Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/renal ischemia-reperfusion injury.ELISA,immunohist ochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb.RESULTS:Hepatic/renal function insuffic-iency, up-regulated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemia-reperfusion injury, while these changes became less conspi-cuous in animals treated with anti-P-selectin mAb.CONCLUSION:P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approa-ch for the prevention and treatment of hepatic/renal ischemia reperfusion injury.
authors.forename: Pei
authors.initials: P
authors.lastname: Wu
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Zhang
authors.forename: Jin-Lian
authors.initials: JL
authors.lastname: Chen
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Chen
authors.forename: De-Chang
authors.initials: DC
authors.lastname: Dong