Establishment of an animal model of chronic atrophic gastritis and a study on the factors inducing atrophy.
OBJECTIVE
To establish a rat model of chronic atrophic gastritis and explore the factors inducing atrophy.


METHODS
In accordance with repeated orthogonal design of L8(2(7)), 60% alcohol and 20 mmol/L sodium deoxycholate (served as factor A), 0.05%-0.1% ammonia water (factor B), 0.05% indomethacin (factor C) were given, alone or in combination, to rats in three experiments for 3 months, 6 months or 9 months respectively. Then the rats were dissected, and their pathologic changes of the gastric mucosa were assessed.


RESULTS
Typical signs of chronic atrophic gastritis (CAG) were found in all rats which were treated with factor A, B, C alone or in combination for 6 or 9 months. No significant difference of pathologic changes of gastric mucosa was found between the rats treated for 6 months and those for 9 months. No obvious CAG signs were found in the rats treated with factor A, B, C for 3 months.


CONCLUSIONS
Sixty percent of alcohol, 20 mmol/L sodium deoxycholate, 0.05%-0.1% ammonia water and 0.05% indomethacin given to Sprague-Dawley rats for 6 months can successfully establish the animal model of CAG. Prolongation of the model-establishment time is not able to further facilitate the atrophy of gastric mucosa.
Si
J
J
Department of Gastroenterology, Sir Run Run Shaw Affiliated Hospital of Zhejiang University, Hangzhou 310016, China.
Zhou
W
W
Wu
J
J
Cao
Q
Q
Xiang
Z
Z
Jiang
L
L
Lü
W
W
Huang
H
H
D000641:Ammonia / Q000009:adverse effects; D000818:Animals; D001284:Atrophy / Q000139:chemically induced; D003840:Deoxycholic Acid / Q000008:administration & dosage; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D005757:Gastritis, Atrophic / Q000139:chemically induced*; D007213:Indomethacin / Q000008:administration & dosage; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D003840:Deoxycholic Acid; D000431:Ethanol; D000641:Ammonia; D007213:Indomethacin