Medium alterations improve regrowth of sweet potato (Ipomoea batatas [L.] lam.) shoot tips cryopreserved by vitrification and encapsulation-dehydration.
In vitro grown sweet potato (Ipomoea batatas [L.] Lam.) shoot tips were successfully cryopreserved by both solution based and encapsulation-dehydration vitrification methods. Improved recovery medium enhanced recovery for both vitrification procedures. The effects of sucrose preculture, cryoprotectant preculture and post-warm recovery media on regrowth following LN exposure were investigated. Sucrose preculture was critical for the survival of sweet potato shoot tips cooled to ca. -200 degrees C. Cryoprotectant preculture with 2 M glycerol + 0.4 M sucrose before dehydration with PVS2 gave the highest recovery following LN exposure. The viability of cooled samples following culture on ammonium-free MS medium for 5 days was increased three-fold over those cultured on MS medium. The improvement in recovery by altering post-warming conditions suggests that cryoinjury is not always lethal and can be ameliorated by suitable culture conditions.
Pennycooke
J C
JC
Department of Horticulture and Landscape Arch., Colorado State University, Fort Collins, CO 80523, USA.
Towill
L E
LE
D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology*; D003681:Dehydration; D027723:Ipomoea batatas / Q000254:growth & development*; D018520:Plant Shoots / Q000187:drug effects* / Q000254:growth & development; D020127:Recovery of Function; D013395:Sucrose / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D015996:Survival Rate
D003451:Cryoprotective Agents; D003470:Culture Media; D013501:Surface-Active Agents; D013395:Sucrose