[Loss of heterozygosity on chromosome 3 in sporadic colorectal carcinoma].
OBJECTIVE
To study the loss of heterozygosity (LOH) on chromosome 3 in sporadic colorectal carcinoma (SCRC) and to explore the possible CRC related genes or loci.


METHODS
LOH at 13 microsatellite loci spanning chromosome 3 were detected in 83 cases with SCRC.


RESULTS
At least two loci LOH on chromosome 3 were detected in 29 of 74 (39%) cases. In 27 of 74 (37%) cases at least one locus LOH was detected on 3p, and in 39 of 74 (53%) cases at least one of 9 loci had LOH on 3q. Of the 13 loci examined, D3S1300 (3p14.2) was identified as the locus with most frequent LOH. 3q LOH was found to occur in distal CRCs more often than those proximal. Tumors with 3p and/or D3S1300 LOH had a tendency of local invasion, and occurred more often in patients older than 50 years.


CONCLUSIONS
LOH on chromosome 3, dispersed with high frequency at several loci, was correlated with clinico-pathology of SCRC, indicating the existence of SCRC related genes on it. The discovery of D3S1300 locus with highly frequent LOH suggested that FHIT gene located in this region might act as a candidate tumor suppressor gene in the development of colorectal carcinoma.
Peng
Z
Z
Department of General Surgery, Shanghai First People's Hospital, Shanghai 200080, China.
Ling
Y
Y
Bai
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002893:Chromosomes, Human, Pair 3; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged