Marked increase in number of dendritic cells in autoimmune-prone (NZW x BXSB)F1 mice with age.
Here, we report that the number of CD11c(+)CD3(-) B220(-) cells increases in autoimmune-prone male (NZW x BXSB)F1 (W/BF1) mice with age. The CD11c(+)CD3(-)B220(-) cells from W/BF1 mice show a typical stellate shape and induce the proliferation of T cells. In the CD11c(+)CD3(-)B220(-) cells from W/BF1 mice, CD11b (Mac-1alpha), NK 1.1, and CD95 (Fas) are upregulated in comparison with normal mice, while the expression of CD8alpha, CD117 (c-kit), CD135 (Flk-2/Flt-3), and Sca-1 decreases. There is a significant increase in Flt-3L (FL) mRNA in the bone marrow of W/BF1 mice with age. Moreover, activated hemopoietic cells express high levels of FL. The injection of CD11c(+)CD3(-)B220(-) cells from old W/BF1 mice to young W/BF1 mice transiently induces autoimmune disease (thrombocytopenia). These results suggest that hyperproduction of FL from activated hemopoietic cells induces a dramatic increase in the number of dendritic cells in aged W/BF1 mice, followed by the acceleration of autoimmunity.
Adachi
Yashusi
Y
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, Japan.
Taketani
Shigeru
S
Toki
Junko
J
Ikebukuro
Kazuya
K
Sugiura
Kikuya
K
Oyaizu
Haruki
H
Yasumizu
Ryoji
R
Tomita
Minoru
M
Kaneda
Hiroyuki
H
Amoh
Yasuo
Y
Ito
Tomoki
T
Okigaki
Mitsuhiko
M
Inaba
Muneo
M
Ikehara
Susumu
S
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D018845:CD11 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D016827:CD8 Antigens / Q000096:biosynthesis; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004705:Endocytosis; D005434:Flow Cytometry; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis; D051379:Mice; D010587:Phagocytosis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013921:Thrombocytopenia / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D019014:fas Receptor / Q000096:biosynthesis; D051941:fms-Like Tyrosine Kinase 3
D018845:CD11 Antigens; D016827:CD8 Antigens; C431698:CD8alpha antigen; D016167:Integrin alphaXbeta2; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D019014:fas Receptor; C084881:flt3 ligand protein; C497971:Flt3 protein, mouse; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases; D051941:fms-Like Tyrosine Kinase 3