title: Lactate concentrations in human skeletal muscle biopsy, microdialysate and venous blood during dynamic exercise under blood flow restriction.
abstract: The intramuscular microdialysate lactate concentration during dynamic exercise with various degrees of blood flow restriction and its relation to lactate concentration in skeletal muscle biopsy and venous blood were studied. Nine healthy males performed three one-legged knee extension exercises (Ex 1-3). Blood flow was restricted stepwise by applying supra-atmospheric pressure over the working leg. Microdialysate mean (range) lactate concentrations at the end of the exercise periods were 3.2 (0.5-6.6), 4.4 (1.1-9.8) and 7.9 (1.1-11.6) mmol.l(-1)during unrestricted, moderately restricted and severely restricted blood flow respectively. There was a significant correlation between microdialysate and venous lactate concentrations at the end of all three exercise periods. Microdialysate lactate concentration correlated significantly to skeletal muscle biopsy lactate concentration at the end of Ex 1. In conclusion, microdialysate lactate concentration in the working muscle increased step-wise with increasing blood flow restriction. It showed a better correlation to venous than to muscle biopsy lactate, which is possibly partly explained by the characteristics of diffusion between body compartments and differences in time resolution between the methods used.
authors.affiliation: Department of Vascular Surgery, Karolinska Hospital, 171 76 Stockholm, Sweden. Goran.Lundberg@kirurgi.ki.se
authors.forename: G
authors.initials: G
authors.lastname: Lundberg
authors.forename: P
authors.initials: P
authors.lastname: Olofsson
authors.forename: U
authors.initials: U
authors.lastname: Ungerstedt
authors.forename: E
authors.initials: E
authors.lastname: Jansson
authors.forename: C J
authors.initials: CJ
authors.lastname: Sundberg
authors.forename: C
authors.initials: C
authors.lastname: Sundberg
mesh_terms: D000328:Adult; D001706:Biopsy; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007511:Ischemia / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood*; D016014:Linear Models; D008297:Male; D017551:Microdialysis; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000166:cytology / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology*; D012039:Regional Blood Flow; D014508:Urea / Q000378:metabolism; D014680:Veins
chemical_list: D019344:Lactic Acid; D014508:Urea; D005947:Glucose; D005990:Glycerol