Methyl jasmonate-induced stimulation of sarcoplasmic reticulum Ca(2+)-ATPase affects contractile responses in rat slow-twitch skeletal muscle.
The purpose of this study was to determine whether methyl jasmonate, a stimulator of Ca(2+)-adenosine triphosphatase (ATPase) activity of the purified ATPase from fast-twitch skeletal muscle, could affect contractile responses in small bundles of rat isolated slow-twitch (soleus) fibers. In saponin-skinned fibers, sarcoplasmic reticulum (SR) Ca(2+) loading was performed in pCa 7.0 solution. The amount of Ca(2+) taken up was monitored by use of the amplitude of contraction following application of 10 mM caffeine. Results indicate that the increased loading rate in the presence of methyl jasmonate is likely due to stimulation of the SR Ca(2+)-ATPase. In Triton-skinned fibers, the myofibrillar Ca(2+) sensitivity was not changed by methyl jasmonate (50-200 microM). In intact fibers, the amplitude and the time constant of relaxation of twitch and potassium contracture were reversibly reduced after 2 min of application of methyl jasmonate at a concentration of up to 125 microM. At higher concentrations (>150 microM), effects were not reversible. In the presence of methyl jasmonate (100 microM), the relationship between the amplitude of potassium contractures and the membrane potential shifted to more positive potentials, whereas the steady-state inactivation curve was unchanged. These observations suggest that methyl jasmonate has no effect on voltage sensors. Taken together, our results show that methyl jasmonate is a potent, reversible, and specific stimulator of the SR Ca(2+) pump in slow-twitch skeletal muscle and is an extremely valuable pharmacological tool for improving relaxation and studying calcium-signaling questions.
Joumaa
Wissam H
WH
Laboratoire de Physiologie Générale, Centre National de la Recherche Scientifique UMR 6018, Université de Nantes, Faculté des Sciences et des Techniques, Nantes, France.
Bouhlel
Aicha
A
Même
William
W
Léoty
Claude
C
D000085:Acetates / Q000494:pharmacology*; D000465:Algorithms; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002469:Cell Separation; D003517:Cyclopentanes / Q000494:pharmacology*; D004594:Electrophysiology; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects* / Q000201:enzymology; D009126:Muscle Relaxation / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*; D013268:Stimulation, Chemical
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate; D000252:Calcium-Transporting ATPases; D011188:Potassium; D002118:Calcium