[Experimental therapy of adenovirus-transferred antisense c-myc on hepatocellular cancer cell].
OBJECTIVE
To investigate the therapeutic effects of Adenovirus-transferred antisense c-myc (Ad-Asmyc) on hepatocellular cancer cells and its mechanism.


METHODS
Human hepatocellular cancer cell line BEL-7402 and QSG-7701 were transfected with Ad-Asmyc. Cell growth curve and coloning formation were used to investigate the therapeutic effects of Ad-Asmyc in Vitro. The treating effects of Ad-Asmyc on transplanted tumor in nude mice was also studied. RT-PCR and Western blot were used to detect relating gene expression. Flowcytometry, DNA ladder and tunnel assay were used to study the cell cycle and apoptosis.


RESULTS
The expression of c-myc in cancer cells was reduced after infected Ad-Asmyc. Ad-Asmyc had obviously inhibiting effects on the growth of two hepatocellular carcinoma cell lines in vitro and also in vivo. It can induced cell cycle arrest (G1 arrest) and apoptosis.


CONCLUSIONS
Ad-Asmyc can inhibit HCC cell proliferation and induce apoptosis, so has obvious therapeutic effects on hepatocellular cancer cells.
Zhang
H
H
Department of Abdominal Surgery, Cancer Hospital, National Laboratory of Molecular Oncology, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, 100021 China.
Lin
C
C
Shao
Y
Y
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors* / Q000235:genetics; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
D016376:Oligonucleotides, Antisense; D016271:Proto-Oncogene Proteins c-myc