title: IN02, a positive regulator of lipid biosynthesis, is essential for the formation of inducible membranes in yeast.
abstract: Expression of the 180-kDa canine ribosome receptor in Saccharomyces cerevisiae leads to the accumulation of ER-like membranes. Gene expression patterns in strains expressing various forms of p180, each of which gives rise to unique membrane morphologies, were surveyed by microarray analysis. Several genes whose products regulate phospholipid biosynthesis were determined by Northern blotting to be differentially expressed in all strains that undergo membrane proliferation. Of these, the INO2 gene product was found to be essential for formation of p180-inducible membranes. Expression of p180 in ino2Delta cells failed to give rise to the p180-induced membrane proliferation seen in wild-type cells, whereas p180 expression in ino4Delta cells gave rise to membranes indistinguishable from wild type. Thus, Ino2p is required for the formation of p180-induced membranes and, in this case, appears to be functional in the absence of its putative binding partner, Ino4p.
authors.affiliation: Department of Biological Chemistry, University of California Los Angeles School of Medicine, Los Angeles, CA 90024, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Block-Alper
authors.forename: Paul
authors.initials: P
authors.lastname: Webster
authors.forename: Xianghong
authors.initials: X
authors.lastname: Zhou
authors.forename: Lubica
authors.initials: L
authors.lastname: Supeková
authors.forename: Wing Hung
authors.initials: WH
authors.lastname: Wong
authors.forename: Peter G
authors.initials: PG
authors.lastname: Schultz
authors.forename: David I
authors.initials: DI
authors.lastname: Meyer
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008856:Microscopy, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D010743:Phospholipids / Q000096:biosynthesis*; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D014157:Transcription Factors
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D010743:Phospholipids; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C095723:INO2 protein, S cerevisiae