[Significance of expression and secretion of interleukin-8 in ependymal tumor].
OBJECTIVE
To detect the expression and secretion of IL-8 in the ependymal tumor tissue and in primarily cultured cells, and to explore the relationship between IL-8 and ependymal tumor.


METHODS
Expression of IL-8 in 50 ependymal tumor specimens was examined by immunohistochemistry. The ependymal tumor tissue obtained during operation was primarily cultured and IL-8 secreted by cultured cells were examined by clouble antibody 5 and which ELISA. The relations of expression of IL-8 to ependymal tumors of different histological degree, and invasiveness was analyzed statistically.


RESULTS
All the ependymal tumor tissue expressed IL-8, but the number of IL-8 positive cells in the anaplastic and malignant ependymoma was evidently larger than that in ependymoma (P < 0.01). All the cells cultured from the ependymal tumor secreted IL-8, but the liquid supernatant of anaplastic and malignant ependymoma cells contained more IL-8 than that of ependymoma cells (P < 0.01). The expression and secretion of IL-8 had no close relation to age and sex of patient.


CONCLUSIONS
The expression and secretion of IL-8 may be involved in the progression of ependymal tumor, so IL-8 can be used as an indicator to detect the malignant degree of ependymal tumor.
Gu
J
J
Institute of Neurosurgery of PLA, Xijing Hospital; Department of Physiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China.
Zhang
X
X
Li
X
X
D000293:Adolescent; D000328:Adult; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002648:Child; D004806:Ependymoma / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged
D016209:Interleukin-8