[Transgenic loach produced by using sperm cells mediated by high molecules].
The pCH110 plasmid DNA which has a marker gene LacZ controlled by a SV40 promoter was mixed with a kind of high molecule of dendrimers(cyclic core dendrimer) and then formed a DNA/dendrimer complex. Loach sperms were incubated with the DNA/dendrimer complex. Then in situ hybridization was used to detect whether the sperm cells captured the foreign DNA or not. Results show that the number of sperm capturing foreign DNA via dendrimers as vectors was obviously increased. The sperms were mixed with eggs for in vitro fertilization. PCR was used to detect the transgenic loach fries. Obvious expression of LacZ gene in the head of transgenic loach were observed by analysis through LacZ histochemical staining of embryos or fries.
Yang
K
K
Center of Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, China.
Cheng
H H
HH
Guo
Y Q
YQ
Zhou
R J
RJ
D000818:Animals; D030801:Animals, Genetically Modified; D003531:Cypriniformes / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D017403:In Situ Hybridization; D008297:Male; D013094:Spermatozoa / Q000378:metabolism*