Effect of IFN-gamma on expression of HLA in bare-lymphocyte syndrome-like cell line HAJ.
We compared HLA antigen expression on new B-lymphoblastoid cell line (B-LCL) HAJ with that on B-LCLs expressing normal HLA levels as well as on B-LCLs derived from bare lymphocyte syndrome (BLS) patients and in vitro mutated B-LCLs of BLS-like phenotype. HAJ cells had no expression of HLA class II and low expression of class I antigens similarly to some of BLS B-LCLs, although HAJ cell line was derived from lymphocytes of HLA class I- and class II-normally expressing donor. HAJ cells displayed B lymphocyte markers, surface immunoglobulin and CD19. Culture of HAJ cells in the presence of interferon y resulted in HLA class I antigen upregulation, but did not restore class II expression. The cell line HAJ may prove useful for studies on factors influencing HLA class I cell surface expression.
Nowak
I
I
Department of Clinical Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Pochroń
B
B
Kozlowska
A
A
Dubis
J
J
Kuśnierczyk
P
P
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers; D002460:Cell Line; D005434:Flow Cytometry; D005805:Genes, MHC Class I / Q000276:immunology; D005802:Genes, MHC Class II / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016511:Severe Combined Immunodeficiency / Q000276:immunology*
D015415:Biomarkers; D006680:HLA Antigens; D007371:Interferon-gamma