Cooperative interaction between interleukin 10 and galectin-3 against liver ischemia-reperfusion injury.
OBJECTIVE
To investigate a possible interaction between interleukin 10 (IL-10) and galectin-3 for protection of human breast carcinoma BT549 cells against liver ischemia-reperfusion-induced cytotoxicity, we used a liver/tumor coculture system. We also used IL-10-deficient C57BL/6 [IL-10(-/-)] mice and their wild-type C57BL/6 [IL-10(+/+)] mice to examine these interactions.


RESULTS
More than 90% of galectin-3 cDNA-transfected BT549 cells (BT549-Gal3) survived after 24-h coculture with C57BL/6 [IL-10(+/+)] liver fragments isolated after ischemia. In contrast, approximately 70% of control vector-transfected BT549 cells (BT549-Neo) showed metabolic death after culture with liver fragment. However, when the ischemic liver from IL-10 (-/-) mice was used, BT549-Gal3 did not exhibit enhanced survival against ischemia-reperfusion-induced cytotoxicity.


CONCLUSIONS
These data suggest that IL-10 and galectin-3 cooperatively interact to protect cells from ischemia-reperfusion injury.
Lee
Yong J
YJ
Department of Pharmacology, Cancer Institute, University of Pittsburgh, Pennsylvania 15213, USA. Leeyj@msx.upmc.edu
Song
Young K
YK
D000818:Animals; D000943:Antigens, Differentiation / Q000502:physiology*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000517:prevention & control; D002470:Cell Survival; D000431:Ethanol / Q000031:analogs & derivatives*; D005260:Female; D005455:Fluorescent Antibody Technique; D037502:Galectin 3; D006801:Humans; D016753:Interleukin-10 / Q000502:physiology*; D008107:Liver Diseases / Q000378:metabolism* / Q000517:prevention & control; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015427:Reperfusion Injury / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D037502:Galectin 3; D016753:Interleukin-10; C062527:tribromoethanol; D000431:Ethanol