title: Role of menaquinones in Fe(III) reduction by membrane fractions of Shewanella putrefaciens.
abstract: Two Tn5-generated mutants of Shewanella putrefaciens with insertions in menD and menB were isolated and analyzed. Both mutants were deficient in the use of several terminal electron acceptors, including Fe(III). This deficiency was overcome by the addition of menaquinone (vitamin K(2)). Isolated membrane fractions from both mutants were unable to reduce Fe(III) in the absence of added menaquinone when formate was used as the electron donor. These results indicate that menaquinones are essential components for the reduction of Fe(III) by both whole cells and purified membrane fractions when formate or lactate is used as the electron donor.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211, USA. daads@uwm.edu
authors.forename: Daad A
authors.initials: DA
authors.lastname: Saffarini
authors.forename: Seth L
authors.initials: SL
authors.lastname: Blumerman
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Mansoorabadi
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D004579:Electron Transport; D005290:Ferric Compounds / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009243:NAD / Q000378:metabolism; D020614:Shewanella putrefaciens / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D024482:Vitamin K 2 / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005290:Ferric Compounds; C446051:MenD protein, Shewanella putrefaciens; D009243:NAD; D024482:Vitamin K 2; D019883:Alkyl and Aryl Transferases; C412723:phytyltransferase