title: Clinical trial of in-situ hybridization method for the rapid diagnosis of sepsis.
abstract: We evaluated the utility of in-situ hybridization (ISH) for the rapid diagnosis of sepsis. We applied this approach to polymorphonuclear neutrophil (PMN)-rich smears from patients with suspected bacterial infection. Positive results by ISH were obtained in the smears of 123 of 292 patients (42%), while only 32 of the 292 (11%) were positive by blood culture. These findings indicate that ISH is almost four times more sensitive than the culture method for the detection of sepsis. ISH results are obtained within 1 day, while 1 day to 2 weeks is required for the results of blood culture. Blood culture and ISH methods detected the same bacteria in two patients. ISH also successfully identified the same bacteria in blood and PMN-rich body fluid (bronchoalveolar lavage samples) in 6 patients. In 19 patients, ISH of blood detected the same bacteria as those found in subcultures from other sources (e.g., stool, sputum, nasal cavity). We discuss these results in comparison with blood culture results in terms of evaluating a rapid approach to the management of patients with sepsis.
authors.affiliation: Department of Microbiology and Medicine, Division of Clinical Pharmacology, Institute of Medical Science, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki 216-8511, Japan. j2simada@marianna-u.ac.jp
authors.forename: J.
authors.initials: J
authors.lastname: Shimada
authors.forename: Izumi
authors.initials: I
authors.lastname: Hayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Inamatsu
authors.forename: Masashi
authors.initials: M
authors.lastname: Ishida
authors.forename: Shigetomi
authors.initials: S
authors.lastname: Iwai
authors.forename: Sadao
authors.initials: S
authors.lastname: Kamidono
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kurosawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Masaoka
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsumoto
authors.forename: Morito
authors.initials: M
authors.lastname: Monden
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Morii
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nakashio
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Narita
authors.forename: Osamu
authors.initials: O
authors.lastname: Sakai
authors.forename: Gotaro
authors.initials: G
authors.lastname: Toda
authors.forename: Kihachiro
authors.initials: K
authors.lastname: Shimizu
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ueda
authors.forename: Tsuneya
authors.initials: T
authors.lastname: Ohno