[Effect of Jiunaoning injection on overload of intracellular free calcium of cerebral cortex induced by glutamic acid or 5-hydroxytryptamine in fetal rats].
OBJECTIVE
To explore the effect of Jiunaoning Injection (JNNI) on overload of intracellular free calcium of cerebral cortex induced by glutamic acid or 5-hydroxytryptamine (5-HT) in fetal rats.


METHODS
Double wavelength spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular calcium ions ([Ca2+]i) was used to measure the changes of [Ca2+]i in instantly separated cortical nerve cells of newborn rats affected by glutamic acid or 5-HT, as well as the interference of JNNI on it.


RESULTS
Glutamic acid or 5-HT could elevate the intracellular [Ca2+]i dose-dependently and induce [Ca2+]i overload. JNNI could restrain the elevation markedly so as to protect the neurocytes from injury of glutamic acid and 5-HT.


CONCLUSIONS
The therapeutic effect of JNNI in treating apoplexy is related with its action in suppressing the toxic substances as glutamic acid and 5-HT, restraining the opening of receptor dependent calcium channel, reducing the external cellular calcium influx and preventing the calcium overload effectively.
Huang
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S
SUN Sulun Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing (100091).
Huang
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D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012702:Serotonin Antagonists / Q000494:pharmacology
D004365:Drugs, Chinese Herbal; D012702:Serotonin Antagonists; D018698:Glutamic Acid; D002118:Calcium