Core 2 oligosaccharides mediate eosinophil and neutrophil peritoneal but not lung recruitment.
We have investigated the importance of cell-surface serine- and/or threonine-linked oligosaccharide adhesion molecules synthesized by the Golgi enzyme core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GlcNAcT) in mediating eosinophil trafficking to the lung in studies utilizing C2GlcNAcT-I-deficient mice. The number of bronchoalveolar eosinophils, the number of lung eosinophils, and airway responsiveness to methacholine were not significantly different in C2GlcNAcT-I-deficient compared with wild-type mice sensitized and challenged by inhalation with ovalbumin. C2GlcNAcT-I-deficient mice do not demonstrate defects in neutrophil trafficking to the lung in response to lipopolysaccharide (LPS). In contrast, ragweed-sensitized C2GlcNAcT-I-deficient mice exhibit significantly reduced eosinophil trafficking to the peritoneal cavity in response to ragweed peritoneal challenge. C2GlcNAcT-I-deficient mice also have significantly reduced neutrophil trafficking to the peritoneal cavity in response to LPS challenge. Overall, these studies demonstrate an important role for serine/threonine-linked oligosaccharides synthesized by the Golgi enzyme C2GlcNAcT-I in eosinophil and neutrophil trafficking to the peritoneum but not for eosinophil or neutrophil trafficking to the lung.
Broide
David H
DH
Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA. dbroide@ucsd.edu
Miller
Marina
M
Castaneda
Diego
D
Nayar
Jyothi
J
Cho
Jae Youn
JY
Roman
Mark
M
Ellies
Lesley G
LG
Sriramarao
P
P
D000818:Animals; D001249:Asthma / Q000276:immunology / Q000378:metabolism; D001853:Bone Marrow / Q000201:enzymology; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000378:metabolism; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D002465:Cell Movement / Q000276:immunology*; D004804:Eosinophils / Q000166:cytology* / Q000201:enzymology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006056:Golgi Apparatus / Q000201:enzymology; D008168:Lung / Q000166:cytology / Q000276:immunology; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000201:enzymology; D009844:Oligosaccharides / Q000378:metabolism*; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism; D011014:Pneumonia / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016085:Bronchoconstrictor Agents; D009844:Oligosaccharides; D016210:Methacholine Chloride; D017351:N-Acetylglucosaminyltransferases; C053024:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase