Study of microdeletions in the Y chromosome of infertile men with idiopathic oligo- or azoospermia.
OBJECTIVE
To determine the relationships between idiopathic oligo- or azoospermia and microdeletions of the Y chromosome.


METHODS
Eighteen Y-linked sequence-tagged sites (STSs) in AZF (Azoospermia Factor) region were screened by means of multiplex PCR (Polymerase Chain Reaction) in 50 idiopathic infertile men, including 16 patients with azoospermia, 13 severe oligospermia, and 21 oligospermia.


RESULTS
Microdeletions in the genomic DNA were observed in 8 of 50 cases, 3 with azoospermia, I severe oligospermia, and 4 oligospermia. Total deletion rate was 16.0% (8/50). The deletion regions were concentrated on AZFd and AZFc.


CONCLUSIONS
Microdeletions of the Y chromosome are an important cause for idiopathic oligo- or azoospermia. Multiplex PCR is a useful technique for detecting the microdeletions. To avoid transmission to their offspring, patients with idiopathic oligo- or azoospermia should be screened for microdeletions of the Y chromosome before ICSI treatment for infertility.
Yao
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G
Department of Obstetrics and Gynecology, The Third Hospital, Peking University, Beijing, China. guimei2613@yahoo.com.cn
Chen
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G
Pan
T
T
D000328:Adult; D002872:Chromosome Deletion; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000209:etiology / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*