Characterization of genes homologous to the general stress-inducible gene gls24 in Enterococcus faecalis and Lactococcus lactis.
Three genes (Ef0604, ymgG, and ytgH) were identified as homologous to the gls24 gene (encoding the general stress protein Gls24) in Enterococcus faecalis V583 and Lactococcus lactis IL1403 genomes sequences, and therefore are part of the 'gls24 family'. The other proteins encoded by the different surrounding genes in each of their respective operons are also highly similar. Our transcriptional analysis showed that Ef0604 and ymgG are not induced under the stress conditions tested. On the other hand, ytgH is induced at the onset of glucose starvation and by heat, ethanolic, osmotic, and CdCl(2) stresses. The transcriptional start site of this operon was identified and the promoter region appears to contain an 'extended -10 Box'. Moreover, the over-expression under several stress conditions of the YtgH protein observed on 2D gel electrophoresis confirms that it corresponds to a general stress protein in L. lactis.
Giard
Jean-Christophe
JC
Laboratoire de Microbiologie de l'Environnement, Université de Caen, 14032 Cedex Caen, France. giard@ibba.unicaen.fr
Verneuil
Nicolas
N
Auffray
Yanick
Y
Hartke
Axel
A
D001426:Bacterial Proteins / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000235:genetics*; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D013294:Lactococcus lactis / Q000235:genetics*; D009876:Operon; D011401:Promoter Regions, Genetic; D020543:Proteome; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; C412463:Gls24 protein, Enterococcus faecalis; D006360:Heat-Shock Proteins; D020543:Proteome