Purification and characterization of two lectins from a toxic moray, Gymnothrax javanicus.
Two lectins, Gymnothrax javanicus lectin-I (GJL-I) and Gymnothrax javanicus lectin-II (GJL-II) were isolated from the stomach and intestine, and the liver, respectively, of a toxic moray eel, Gymnothrax javanicus. GJL-I is a polymer of two heterogeneous subunits of 67 and 51 kDa. In a hemagglutination inhibition assay, it had sugar-binding specificity toward lactose and lactulose among the mono- or oligo-saccharides and bovine submaxillary mucin (BSM) among the glycoproteins tested. The lectin stimulated nerve growth factor (NGF) synthesis by astroglial cells. GJL-II was a polymer of subunit of 41 kDa. This lectin had N-acetyllactosamine binding specificity.
Kawagishi
H
H
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan. achkawa@agr.shizuoka.ac.jp
Yasui
M
M
Uno
A
A
Murata
T
T
Usui
T
T
Furukawa
S
S
D000596:Amino Acids / Q000032:analysis; D000606:Amino Sugars / Q000378:metabolism; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D004524:Eels / Q000378:metabolism*; D006385:Hemagglutination Inhibition Tests; D066298:In Vitro Techniques; D007422:Intestines / Q000737:chemistry; D007785:Lactose / Q000378:metabolism; D007792:Lactulose / Q000378:metabolism; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D009077:Mucins / Q000378:metabolism; D020932:Nerve Growth Factor / Q000096:biosynthesis; D021122:Protein Subunits; D051381:Rats; D013270:Stomach / Q000737:chemistry
D000596:Amino Acids; D000606:Amino Sugars; D037102:Lectins; D009077:Mucins; D021122:Protein Subunits; C000458:N-acetyllactosamine; D007792:Lactulose; D020932:Nerve Growth Factor; D007785:Lactose