Hemodynamic effects of peritoneal dialysis solutions on the rat peritoneal membrane: role of acidity, buffer choice, glucose concentration, and glucose degradation products.
Conventional peritoneal dialysis fluids (PDF) are unphysiologic because of their hypertonicity, high glucose and lactate concentrations, acidic pH, and presence of glucose degradation products (GDP). Long-term exposure to conventional PDF may cause functional and structural alterations of the peritoneal membrane. New PDF have a neutral pH, a low GDP content, and contain bicarbonate or lactate as the buffer. Intravital microscopy was used to analyze the vasoactive effects of conventional and new PDF on the rat peritoneal membrane. A conventional, acidic pH, lactate-buffered 4.25% glucose PDF induced maximal vasodilation of mesenteric arteries, resulting in a doubling of the arteriolar flow and a 20% increase of the perfused capillary length per area. The hemodynamic effects of conventional PDF were similar after pH-adjustment with NaOH, indicating that acidity per se is not essential for the changes. Superfusion by a pH-neutral, lactate-buffered PDF with low GDP content caused only a transient arterial vasodilation despite continuous exposure, with a commensurate effect on arteriolar flow and capillary recruitment. Application of a pH-neutral, bicarbonate-buffered PDF with low GDP content did not affect the hemodynamic parameters. Resterilization of the bicarbonate solution increased GDP levels and completely restored the vasodilatory capacity. The corresponding 1.5% glucose PDF induced similar but less pronounced changes. Conventional PDF have important vasoactive effects on the peritoneal circulation, mainly because of the presence of GDP and transiently because of high lactate concentrations. Capillary recruitment may increase effective peritoneal vascular surface area. In addition, chronic vasodilation may induce structural adaptations in the blood vessel wall, contributing to vascular sclerosis. PDF with reduced GDP content induce no major hemodynamic effects and may thus have the potential to better preserve peritoneal vascular integrity.
Mortier
Siska
S
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
De Vriese
An S
AS
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
Van de Voorde
Johan
J
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
Schaub
Thomas P
TP
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
Passlick-Deetjen
Jutta
J
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
Lameire
Norbert H
NH
*Renal Unit and Department of Physiology, University Hospital, Gent, Belgium; and Fresenius Medical Care Deutschland GmbH, Bad Homburg, Germany.
D000143:Acids / Q000378:metabolism; D000818:Animals; D002021:Buffers; D015314:Dialysis Solutions / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006439:Hemodynamics / Q000187:drug effects; D008566:Membranes / Q000098:blood supply; D008638:Mesenteric Arteries / Q000187:drug effects / Q000502:physiology; D008833:Microcirculation / Q000187:drug effects; D009994:Osmolar Concentration; D010531:Peritoneal Dialysis, Continuous Ambulatory; D010537:Peritoneum / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation
D000143:Acids; D002021:Buffers; D015314:Dialysis Solutions; D005947:Glucose