Assessment of the neovascular permeability in glioma xenografts by dynamic T(1) MRI with Gadomer-17.
The uptake of Gadomer-17, as probed by fast dynamic T(1) measurements, was used to assess the vascular permeability surface-area product per leakage volume of tissue (k(Tofts)) of human glioma xenografts implanted in mice. With this approach we could discriminate between two types of glioma xenograft lines with a known difference in the perfused vascular architecture and degree of hypoxia. The T(1) data were analyzed according to the Tofts-Kermode compartment model. The fast-growing E102 tumor demonstrated a homogeneous distribution of the vascular permeability surface area across the tumor (mean k(Tofts) value = 0.18 +/- 0.05 min(-1)). The slowly growing E106 tumor showed a more heterogeneous pattern. Three perfused tumor areas with differences in vascular permeability surface area could be distinguished: a well-perfused periphery with high k(Tofts) values (0.24 +/- 0.04 min(-1)), perfused capillaries inside the tumor with low k(Tofts) values (0.108 +/- 0.026 min(-1)), and perfused capillaries adjacent to necrotic regions with high k(Tofts) values (0.29 +/- 0.10 min(-1)). On a different series of tumors, the hypoxic fractions were measured, and these were significantly higher in E106 tumors (0.14 +/- 0.05) compared to tumors of the E102 line (0.03 +/- 0.02).
Verhoye
Marleen
M
Bio Imaging Lab, RUCA, Antwerp, Belgium. maver@ruca.ua.ac.be
van der Sanden
Boudewijn P J
BP
Rijken
Paul F J W
PF
Peters
Hans P W
HP
Van der Kogel
Albert J
AJ
Pée
Gilke
G
Vanhoutte
Greetje
G
Heerschap
Arend
A
Van der Linden
Annemie
A
D000818:Animals; D002199:Capillary Permeability / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D003287:Contrast Media / Q000494:pharmacology*; D005682:Gadolinium; D005910:Glioma / Q000098:blood supply*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D003287:Contrast Media; C402765:gadomer 17; D005682:Gadolinium