Dendroaspis natriuretic peptide-like immunoreactivity and its regulation in rat aortic vascular smooth muscle.
Dendroaspis natriuretic peptide (DNP) is a recently isolated 38 amino acid peptide that shares structural and functional properties with the other members of the natriuretic peptide family. The present study demonstrates the presence of DNP-like immunoreactivity in sections of rat aorta, carotid artery and renal vasculature and tubules. DNP-like immunoreactivity was detected in culture aortic vascular smooth muscle cells and medium and is regulated by endothelin-1, angiotensin II and sodium nitroprusside but not by transforming growth factor-beta. Our observations indicate that DNP elicits a marked inhibitory effect on DNA synthesis in culture rat aortic vascular smooth muscle cells.
Woodard
Geoffrey E
GE
Department of Physiology, University of Cambridge, Downing Street, CB2 3EG, Cambridge, UK. geoffreyw@intra.niddk.nih.gov
Rosado
Juan A
JA
Brown
John
J
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000096:biosynthesis; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002339:Carotid Arteries / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004546:Elapid Venoms / Q000737:chemistry* / Q000378:metabolism; D017815:Elapidae / Q000378:metabolism*; D019332:Endothelin-1 / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000378:metabolism*; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000098:blood supply; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C075597:Dendroaspis natriuretic peptide; D004546:Elapid Venoms; D019332:Endothelin-1; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D016212:Transforming Growth Factor beta; D000804:Angiotensin II; D009599:Nitroprusside; D004247:DNA