[Evaluation of immunochromatography based rapid detection kit of rotavirus and adenovirus].
We investigated the usefulness of Rapidtesta Rota-Adeno (Daiichi Pure Chemicals Co., Ltd., Japan) for rapid detection of group A rotavirus and adenovirus simultaneously using immunochromatography with clinical samples. In investigation of the reaction of the kit to 5 strains of group A rotavirus, 13 strains of adenovirus and other intestinal viruses, specific lines were formed in red to group A rotavirus and blue to adenovirus. No cross reaction was observed with other intestinal virus. In measurement of detection limit, group A rotavirus (SA11) was detected at 10(4.4)TCID50/ml, serotype 3 adenovirus at 10(4.45)TCID50/ml. The detection limit of the kit was similar to other immunochromatographic assay or enzyme immnoassay kits and approximately 10 times higher than that of kits using latex agglutination test. In comparison with other testing kits in clinical samples, the concordance with other immunochromatographic assay was 99.2% (121/122) in group A rotavirus and 98.6% (138/140) in adenovirus. The rate of concordance with latex agglutination test kit was 94.5% (69/73) in group A rotavirus and 92.3% (84/91) in adenovirus. The kit had high rates of concordance with other immunochromatographic assay kits and higher virus detection rates than those of latex agglutination test kits. Rapidtesta Rota-Adeno is able to detect group A rotavirus and adenovirus simply and rapidly. In addition, the two kinds of virus can be easily differentiated by color difference in reaction lines, suggesting that the kit is useful in clinical diagnosis.
Shimizu
H
H
Kawasaki City Institute of Public Health.
Li
L
L
Mitamura
K
K
Okuyama
K
K
Hirai
Y
Y
Ushijima
H
H
D000256:Adenoviridae / Q000302:isolation & purification*; D002845:Chromatography; D006801:Humans; D007841:Latex Fixation Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012401:Rotavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic