Linkage of sugar chains to a fragment peptide of FGF-5S by a chemoenzymatic strategy and changes in the rate of proteolytic hydrolysis.
Various O-linked and N-linked sugar chains were linked enzymatically to a fragment peptide (Leu-Ser-Gln(or Asn)-Val-His-Arg) of FGF-5S. First, galactose was linked with beta-(1-->3)-linkage to GalNAc-linked peptide by a transglycosylation using beta-galactosidase from Bacillus circulans (recombinant). Then sialic acid was linked with the aid of sialyltransferase from rat liver (recombinant) to give NeuAcalpha-(2-->3)-Galbeta-(1-->3)-GalNAc-linked hexapeptide. Further, a sialylated 2-chain biantennary sugar chain was linked by a transglycosylation using endo N-acetyl-beta-D-glucosaminidase from Mucor hiemalis (endo M, recombinant). The activity of DNA synthesis in a fibroblast cell line was increased by this glycosylation. The resistance of the obtained glycopeptides towards proteolytic hydrolysis by rat serum and by five proteases was compared with that of original peptide. The resistance was remarkably enhanced by the glycosylation.
Ajisaka
K
K
Nutrition Science Institute, Meiji Milk Products Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan. kajisaka@mbc.sphere.ne.jp
Miyasato
M
M
Ito
C
C
Fujita
Y
Y
Yamazaki
Y
Y
Oka
S
S
D000818:Animals; D001407:Bacillus; D002851:Chromatography, High Pressure Liquid; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors / Q000737:chemistry* / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D006868:Hydrolysis; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats; D012799:Sialyltransferases / Q000378:metabolism; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000378:metabolism
C496451:Fgf5 protein, rat; D006023:Glycoproteins; D010446:Peptide Fragments; D011134:Polysaccharides; D051521:Fibroblast Growth Factor 5; D005346:Fibroblast Growth Factors; D012799:Sialyltransferases; D001616:beta-Galactosidase; D010447:Peptide Hydrolases