title: Acyl-CoA binding protein expression is fiber type- specific and elevated in muscles from the obese insulin-resistant Zucker rat.
abstract: Accumulation of acyl-CoA is hypothesized to be involved in development of insulin resistance. Acyl-CoA binds to acyl-CoA binding protein (ACBP) with high affinity, and therefore knowledge about ACBP concentration is important for interpreting acyl-CoA data. In the present study, we used a sandwich enzyme-linked immunosorbent assay to quantify ACBP concentration in different muscle fiber types. Furthermore, ACBP concentration was compared in muscles from lean and obese Zucker rats. Expression of ACBP was highest in the slow-twitch oxidative soleus muscle and lowest in the fast-twitch glycolytic white gastrocnemius (0.46 +/- 0.02 and 0.16 +/- 0.005 microg/mg protein, respectively). Expression of ACBP was soleus > red gastrocnemius > extensor digitorum longus > white gastrocnemius. Similar fiber type differences were found for carnitine palmitoyl transferase (CPT)-1, and a correlation was observed between ACBP and CPT-1. Muscles from obese Zucker rats had twice the triglyceride content, had approximately twice the long-chain acyl CoA content, and were severely insulin resistant. ACBP concentration was approximately 30% higher in all muscles from obese rats. Activities of CPT-1 and 3-hydroxy-acyl-CoA dehydrogenase were increased in muscles from obese rats, whereas citrate synthase activity was similar. In conclusion, ACBP expression is fiber type-specific with the highest concentration in oxidative muscles and the lowest in glycolytic muscles. The 90% increase in the concentration of acyl-CoA in obese Zucker muscle compared with only a 30% increase in the concentration of ACBP supports the hypothesis that an increased concentration of free acyl-CoA is involved in the development of insulin resistance.
authors.affiliation: Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Franch
authors.forename: Jens
authors.initials: J
authors.lastname: Knudsen
authors.forename: Bronwyn A
authors.initials: BA
authors.lastname: Ellis
authors.forename: Preben K
authors.initials: PK
authors.lastname: Pedersen
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Cooney
authors.forename: Jørgen
authors.initials: J
authors.lastname: Jensen
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D026261:Diazepam Binding Inhibitor / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005990:Glycerol / Q000378:metabolism; D007333:Insulin Resistance; D018485:Muscle Fibers, Skeletal / Q000145:classification / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; D009765:Obesity / Q000503:physiopathology*; D051381:Rats; D011924:Rats, Zucker; D012016:Reference Values; D014280:Triglycerides / Q000378:metabolism
chemical_list: D026261:Diazepam Binding Inhibitor; D014280:Triglycerides; D000429:Alcohol Oxidoreductases; D064430:NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases; C114725:6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase; D002334:Carnitine O-Palmitoyltransferase; D005990:Glycerol