[BRCA1 gene mutations in early-onset breast cancer].
OBJECTIVE
To detect BRCA1 gene mutations in early-onset breast cancer.


METHODS
We use polymerase chain reaction and single strand conformation polymorphism analysis (PCR-SSCP) to examine the mutation at BRCA1 gene exon 2, 11 and 20 in 10 patients with early-onset breast cancer.


RESULTS
Mutation was found in one cases (24 years old at diagnosis) at nucleotide 3 732, the substitution of a "G" to a "C" in codon 1 205 changed a Gly to a Arg.


CONCLUSIONS
Our data suggest that BRCA1 may have effect on early-onset breast cancer.
Ding
X
X
Peking Union Medical College Hospital, Beijing 100730, China.
Lang
J
J
D000328:Adult; D017668:Age of Onset; D019943:Amino Acid Substitution; D019313:BRCA1 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
D019313:BRCA1 Protein