Adenoviral mediated interleukin-2 gene transfer to human long-term bone marrow stromal cultures.
Human long-term bone marrow stromal cultures (LTBMC) have been transduced to express the immunostimulatory cytokine interleukin-2 (IL-2). LTBMC were established from normal bone marrow and characterized by gross morphology, immunohistochemistry, immunophenotyping, and immunofluorescent staining. A stromal marrow cell (SMC) population expressing the markers CD68, CD29, CD13, and CD54 was identified. These cells were successfully transduced with an adenoviral vector containing the human IL-2 gene. Production of IL-2 was demonstrated for at least 13 days post-transduction and was maximal on day 10 at 19,350 pg (or 38 IU) of IL-2/10(6) cells/24 h. IL-2 expressed by the transduced SMC was demonstrated to be biologically active, resulting in the proliferation of autologous T lymphocytes in a mixed lymphocyte reaction. Autologous human LTBMC can be obtained and manipulated with relative ease and may be a useful vehicle for sustained local cytokine delivery.
Hicks
C
C
Department of Haematology, Prince of Wales Hospital, High Street, Randwick, NSW, 2031, Sydney, Australia. c.hicks@student.unsw.edu.au
Keoshkerian
E
E
French
R
R
Lindeman
R
R
D000256:Adenoviridae / Q000235:genetics*; D015703:Antigens, CD / Q000378:metabolism; D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D007376:Interleukin-2