title: Assessment of bioaerosols in swine barns by filtration and impaction.
abstract: Bioaerosol concentrations inside one naturally ventilated and one mechanically ventilated swine finishing barn were assessed by sampling air using membrane filtration and impaction (six-stage Andersen sampler), and assayed by culture method. The barns, located on the same commercial farm in northeast Kansas, did not show any significant difference (p > 0.05) in concentrations of total and respirable airborne microorganisms. The overall mean total concentrations inside the two barns were 6.6 x 10(4) colony forming units (CFU)/m3 (SD = 3.8 x 10(4) CFU/m3 as measured by filtration and 8.6 x 10(4) CFU/m3 (SD = 5.1 x 10(4) CFU/m3) by impaction. The overall mean respirable concentrations were 9.0 x 10(3) CFU/m3 (SD = 4.1 x 10(3) CFU/m3) measured by filtration and 2.8 x 10(4) CFU/m3 (SD = 2.2 x 10(4) CFU/m3) by impaction. Total and respirable CFU concentrations measured by impaction were significantly (p < 0.05) higher than that by filtration. The persistent strains of microorganisms were various species of the following genera: Staphylococcus, Pseudomonas, Bacillus, Listeria, Enterococcus, Nocardia, Lactobacillus, and Penicillium. It appears that filtration sampling can be used for a qualitative survey of bioaerosols in swine barns while the Andersen sampler is suitable for both quantitative and qualitative assessments.
authors.affiliation: Department of Biological and Agricultural Engineering, 147 Seaton Hall, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Bernardo Z
authors.initials: BZ
authors.lastname: Predicala
authors.forename: James E
authors.initials: JE
authors.lastname: Urban
authors.forename: Ronaldo G
authors.initials: RG
authors.lastname: Maghirang
authors.forename: Sheryll B
authors.initials: SB
authors.lastname: Jerez
authors.forename: Robert D
authors.initials: RD
authors.lastname: Goodband
mesh_terms: D000336:Aerosols / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D015169:Colony Count, Microbial / Q000295:instrumentation; D005374:Filtration; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006799:Housing, Animal; D013552:Swine / Q000382:microbiology*; D014691:Ventilation / Q000592:standards*
chemical_list: D000336:Aerosols