Antisense display: a new method for functional gene screen and its application to angiogenesis-related gene isolation.
We have developed an antisense-oriented method for functional gene screening, which we termed "antisense display." After evaluation in a cell-free system, we applied this method to a cellular system for isolating angiogenesis-related genes and came across a gene previously not described in humans. A screen of 10 mer antisense repertoire led to the identification of a unique 'angiostatic' antisense, and the gene corresponding to it was found to be preferentially expressed in vascular endothelial cells, coding for a putative type-2 membrane protein containing Rab-GAP/TBC and EF-hand-like domains. We named it vascular Rab-GAP/TBC domain-containing protein (VRP). The results obtained thus indicate that the antisense display method is useful for the isolation of functional genes responsible for phenotypes concerned.
Yonekura
H
H
Department of Biochemistry and Molecular Vascular Biology, Kanazawa University Graduate School of Medical Science, Japan.
Yasui
K
K
Sakurai
S
S
Li
H
H
Yamamoto
Y
Y
Yamamoto
H
H
D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D009389:Neovascularization, Pathologic / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense; D009928:Organ Specificity; D014018:Tissue Distribution; D020691:rab GTP-Binding Proteins / Q000235:genetics*
D020319:Oligodeoxyribonucleotides, Antisense; D020691:rab GTP-Binding Proteins