Palmitate and oleate metabolism of rainbow trout in vivo.
The turnover rates of palmitate and oleate were measured in vivo by continuous infusion of 1-[14C]palmitate and 9,10-[3H]oleate in rainbow trout. Our goals were: (1) to quantify the incorporation of a saturated and of a monounsaturated fatty acid into other classes of plasma lipids (neutral lipids, NL, and phospholipids, PL); and (2) to determine whether they could both be used as tracers to quantify fluxes of total non-esterified fatty acids (NEFA). We found that both acids play very different physiological roles because palmitate is preferentially channeled towards plasma PL, whereas oleate is mainly incorporated in circulating NL. Consequently, palmitate is predominantly involved in membrane PL turnover and oleate in the metabolism of circulating NL that may be used to shuttle oxidative fuel in teleosts. Despite this striking difference in their metabolism, palmitate and oleate have flux rates that are proportional to their relative abundance in plasma NEFA (i.e. they have the same fractional turnover rate). They can therefore both be used as reliable tracers to quantify the kinetics of total NEFA.
Weber
Jean Michel
JM
Biology Department, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada K1N 6N5. jmweber@science.uottawa.ca
Brichon
Gérard
G
Bodennec
Jacques
J
Zwingelstein
Georges
G
D000818:Animals; D002247:Carbon Isotopes; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D007700:Kinetics; D019301:Oleic Acid / Q000097:blood / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000378:metabolism*; D010168:Palmitates / Q000097:blood / Q000378:metabolism*; D010743:Phospholipids / Q000097:blood / Q000378:metabolism; D013997:Time Factors
D002247:Carbon Isotopes; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D010168:Palmitates; D010743:Phospholipids; D019301:Oleic Acid