Two "wild-type" variants of Escherichia coli sigma(70): context-dependent effects of the identity of amino acid 149.
The identity of amino acid 149 of Escherichia coli sigma(70) has been reported variably as either arginine or aspartic acid. We show that the behavior of both a region 1.2 deletion and a single-amino-acid substitution at position 122 are greatly affected by the identity of amino acid 149.
Baldwin
Nicole E
NE
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas 77030, USA.
McCracken
Andrea
A
Dombroski
Alicia J
AJ
D019943:Amino Acid Substitution; D000596:Amino Acids; D001216:Asparagine / Q000235:genetics*; D001224:Aspartic Acid / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D014644:Genetic Variation; D017384:Sequence Deletion; D012808:Sigma Factor / Q000235:genetics*; D014158:Transcription, Genetic
D000596:Amino Acids; D012808:Sigma Factor; D001224:Aspartic Acid; D001216:Asparagine; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases