1H NMR analysis for metabolites in serum and urine from rats administrated chronically with La(NO3)3.
1H NMR spectroscopy has been used to assess long-term toxicological effects of a rare earth. Male Wistar rats were administrated orally with La(NO3)3 at doses of 0.1, 0.2, 2.0, 10, and 20 mg/kg body wt, resp., for 3-6 months. Urine was collected at 1, 2, and 3 months and serum samples were taken after 6 months. Numerous low-M(r) metabolites in rats serum and rats urine, including creatinine, citrate, glucose, ketone bodies, trimethylamine N-oxide (TMAO), and various amino acids, were identified on 400- and 500-MHz 1H NMR spectra. La3+-induced renal and liver damage is characterized by an increase in the amounts of the excreted ketone bodies, amino acids, lactate, ethanol, succinate, TMAO, dimethylamine, and taurine and a decrease in citrate, glucose, urea, and allantoin. Information on the molecular basis of the long-term toxicity of La(NO>3)3 was derived from the abnormal patterns of metabolite excretions. An assay of some biochemical indexes and analysis of some enzymes in plasma supported NMR results.
Feng
Jianghua
J
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Li
Xiaojing
X
Pei
Fengkui
F
Chen
Xi
X
Li
Shulei
S
Nie
Yuxiu
Y
D000596:Amino Acids / Q000097:blood / Q000652:urine; D000818:Animals; D019343:Citric Acid / Q000097:blood / Q000652:urine; D005021:Ethylamines / Q000097:blood / Q000652:urine; D007657:Ketone Bodies / Q000097:blood / Q000652:urine; D019344:Lactic Acid / Q000097:blood / Q000652:urine; D007811:Lanthanum / Q000633:toxicity*; D017093:Liver Failure / Q000139:chemically induced / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000139:chemically induced / Q000378:metabolism*
D000596:Amino Acids; D005021:Ethylamines; D007657:Ketone Bodies; C056207:triethylamine-N-oxide; D019343:Citric Acid; C016534:lanthanide nitrate; D019344:Lactic Acid; D007811:Lanthanum