title: Continuous antigen delivery from controlled release implants induces significant and anamnestic immune responses.
abstract: Two continuous delivery injectable silicone implants were tested to determine if they were capable of delivering vaccines in a single shot. The Type A implant delivers antigen in vitro over a 1-month-period and the Type B over several months. Vaccination studies in sheep were designed to compare the responses induced by the Type A and B implants, Alzet mini-osmotic pumps and conventional antigen delivery. A model antigen, avidin, was used along with IL-1beta or alum as adjuvants. Sheep were immunised with various formulations and the titre and isotype of the antigen specific antibodies monitored. The Type B implant induced antibody (Ab) titres of greater magnitude and duration than soluble vaccines or the Type A implant with adjuvant, but only if IL-1beta was included in the formulation. Both implants induced antibodies of IgG1 and IgG2 isotype. A memory response to soluble antigen challenge was induced by the Type B+IL-1beta implant, which was predominantly of an IgG1 isotype.
authors.affiliation: Centre for Animal Biotechnology, School of Veterinary Science, The University of Melbourne, Vic. 3010, Australia. j.kutyla@pgrad.unimelb.edu.au
authors.forename: J M
authors.initials: JM
authors.lastname: Kemp
authors.forename: M
authors.initials: M
authors.lastname: Kajihara
authors.forename: S
authors.initials: S
authors.lastname: Nagahara
authors.forename: A
authors.initials: A
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Brandon
authors.forename: S
authors.initials: S
authors.lastname: Lofthouse
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D016503:Drug Delivery Systems; D004343:Drug Implants; D005260:Female; D007132:Immunoglobulin Isotypes / Q000097:blood; D007260:Infusion Pumps; D007375:Interleukin-1 / Q000494:pharmacology; D012756:Sheep; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000941:Antigens; D004343:Drug Implants; D007132:Immunoglobulin Isotypes; D007375:Interleukin-1; D014612:Vaccines