ADAMs (a disintegrin and metalloproteinase) messenger RNA expression in Helicobacter pylori-infected, normal, and neoplastic gastric mucosa.
Helicobacter pylori is a risk factor for gastric cancer, and bacterial-epithelial interactions may be critical in this association. Studies using complementary DNA arrays indicated that the ADAM (A disintegrin and metalloproteinase) genes in gastric epithelial cells are differentially expressed after bacterial-epithelial interactions. Reverse-transcription polymerase chain reaction analysis of gastric biopsy specimens from patients with and without H. pylori showed that infection was associated with increased expression of ADAM 10 and ADAM 17 (tumor necrosis factor-alpha-converting enzyme) in antral mucosa, but no increases in ADAM 15 and ADAM 20 were observed. Increased ADAM 10 transcripts were observed only in cagA-negative infections. High levels of ADAM 10, ADAM 17, and ADAM 20 transcripts were present in gastric carcinoma. H. pylori stimulated temporal changes in ADAM 10 and ADAM 17 transcripts in gastric epithelial cells. Chronic infection with H. pylori may result in persistent mucosal increases in members of the ADAMs family. ADAMs-mediated ectodomain shedding may have a role in gastric carcinogenesis.
Yoshimura
Tetsuro
T
Molecular Medicine Unit, St. James's University Hospital, Leeds LS9 7TF, United Kingdom. msjjc@stjames.leeds.ac.uk
Tomita
Toshihiko
T
Dixon
Michael F
MF
Axon
Anthony T R
AT
Robinson
Philip A
PA
Crabtree
Jean E
JE
D051722:ADAM Proteins; D000072197:ADAM10 Protein; D000293:Adolescent; D000328:Adult; D000368:Aged; D053829:Amyloid Precursor Protein Secretases; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D013274:Stomach Neoplasms / Q000378:metabolism*
D008565:Membrane Proteins; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; C098660:ADAM15 protein, human; C110998:ADAM20 protein, human; D008666:Metalloendopeptidases; D000072197:ADAM10 Protein; C491236:ADAM10 protein, human