Dominant-negative Rho, Rac, and Cdc42 facilitate the invasion process of Vibrio parahaemolyticus into Caco-2 cells.
To clarify the invasive process of Vibrio parahaemolyticus, an invasion assay was performed using cells expressing dominant negative small GTPases of the Rho family. This assay showed that the dominant negative host phenotype facilitates bacterial invasion, suggesting that the mechanism of V. parahaemolyticus invasion differs from that reported for other invasive bacteria.
Akeda
Yukihiro
Y
Department of Bacterial Infections. Department of Bacterial Toxinology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan. yukihiro@biken.osaka-u.ac.jp
Kodama
Toshio
T
Kashimoto
Takashige
T
Cantarelli
Vlademir
V
Horiguchi
Yasuhiko
Y
Nagayama
Kenichi
K
Iida
Tetsuya
T
Honda
Takeshi
T
D000199:Actins / Q000378:metabolism; D018938:Caco-2 Cells; D006801:Humans; D014736:Vibrio parahaemolyticus / Q000472:pathogenicity*; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000502:physiology*; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000502:physiology*
D000199:Actins; D020764:cdc42 GTP-Binding Protein; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein