Lipocalin-type prostaglandin D synthase in milk: a new biomarker for bovine mastitis.
The whey protein pattern of milk from animals affected by mastitic inflammation was resolved by two-dimensional gel electrophoresis (2D-PAGE) and compared to milk from unaffected cows. Inflammation caused the appearance of four spots aligned at a molecular weight level of 26 kDa and over a pH-region of 5.0 to 6.4. The spots excised from 2D gels were treated with chymotrypsin and the resulting peptides analyzed by MALDI-TOF mass spectrometry and RP-HPLC. All four spots yielded highly similar chymotryptic peptide mass fingerprints as well as chromatographic peak patterns. A database search could identify the four spots as isoforms of the bovine prostaglandin D synthase (PGD-S). In one of the isoforms a defined cysteine residue was shown to be oxidized to a sulfonic acid.
Baeker
R
R
Brandenburg State Laboratory of Veterinary Diagnostics and Food Analysis, Potsdam, Germany.
Haebel
S
S
Schlatterer
K
K
Schlatterer
B
B
D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D019746:Intramolecular Oxidoreductases / Q000032:analysis*; D054834:Lipocalins; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000201:enzymology*; D008892:Milk / Q000201:enzymology*; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015415:Biomarkers; D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase