Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.
The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.
Dincturk
H B
HB
Molecular Biology Research Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK. dincturk@itu.edu.tr
Dunn
A J
AJ
Jones
D H
DH
D015153:Blotting, Western; D003001:Cloning, Molecular; D003208:Concanavalin A / Q000737:chemistry* / Q000235:genetics; D007887:Fabaceae / Q000737:chemistry*; D037121:Plant Lectins; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012995:Solubility
D037121:Plant Lectins; D011498:Protein Precursors; D011994:Recombinant Proteins; D003208:Concanavalin A