[Effect of Saikosaponin-d on up-regulating GR mRNA expression and inhibiting cell growth in human leukemia cells].
OBJECTIVE
To study the effect of Saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and cell growth in HL60 cells.


METHODS
Antiproliferation effects of SSd on HL60 cells were determined by 3H-thymidine incorporation. Cell cycle analysis was also performed by flow cytometry. GRmRNA was analyzed by northern blot analysis.


RESULTS
After 48 hours treatment with 10 micrograms/ml SSd, 3H-thymidine incorporation decreased in HL60 cells and the effect was time and dose dependent. The flow cytometry analysis showed HL60 cells were arrested at G0/G1 phase. The expression of GR mRNA increased.


CONCLUSIONS
SSd exhibits the inhibition effects on cell growth in HL60 cells and could up-regulate the expression of GR mRNA in HL60 cells.
Bu
S
S
Physiology Department, Second Military Medical University, Shanghai (200433).
Xu
J
J
Sun
J
J
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins; D015854:Up-Regulation
D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D