[The genotype of vac A gene of Helicobacter pylori (Hp) and its correlation with the gastroduodenal diseases associated with Hp].
OBJECTIVE
To analyze the expression of vac A gene of Helicobacter pylori (Hp) isolated from Chinese patients and to find out the relationship between the genotype of Hp vac A and the gastroduodenal diseases associated with Hp.


METHODS
Clones of Hp obtained from patients with gastroduodenal diseases were cultured and mRNA extraction was performed with reverse transcriptase polymerase chain reaction (RT-PCR).


RESULTS
92% of the strains isolated in Shanghai (China) was found to be s1/m2. Strains expressing s1/m1 forms were obtained in 4 cases. s2 form was not found.


CONCLUSIONS
The overwhelming majority of strains isolated from Shanghai patients expresses s1/m2 from. The s1/m2 genotype is dispersed in all of the gastroduodenal diseases associated with Hp.
Hou
P
P
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Xu
G
G
Gong
Y
Y
Tu
Z
Z
Li
Z
Z
Ji
X
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D001426:Bacterial Proteins / Q000235:genetics*; D002681:China; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori