Flow injection analysis of doxazosin mesylate using UV-detection.
A flow injection analysis (FIA) of doxazosin mesylate (DOX) using UV detection is described in this study. The best solvent system was found to be consisting of 0.1 mol l(-1) acetate buffer at pH 4 having 10%MeOH. A flow rate of 1 ml min(-1) was pumped and active material was detected at 365 nm. The calibration equation was linear in the range of 1.3 x 10(-5) to 6.4 x 10(-5) mol l(-1). Limit of detection and limit of quantitation were calculated to be 1.6 x 10(-6) and 4 x 10(-6) mol l(-1) with a RSD 1.27 and 1.16% (n=8), respectively. The proposed method was applied to the determination of DOX in the pharmaceutical preparations. The results were compared with those obtained from UV-Spectrophotometry. The results showed that there is a good agreement between FIA method and UV-Spectrophotometry. The validation studies were realised by the related applications and the results were evaluated statistically. According to the results, insignificant difference was observed between the methods.
Altiokka
G
G
Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey. galtiokk@anadolu.edu.tr
Atkosar
Z
Z
D000317:Adrenergic alpha-Antagonists / Q000032:analysis* / Q000737:chemistry; D017292:Doxazosin / Q000032:analysis* / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013607:Tablets
D000317:Adrenergic alpha-Antagonists; D013607:Tablets; D017292:Doxazosin