title: A previously unclassified trypanosomatid responsible for human cutaneous lesions in Martinique (French West Indies) is the most divergent member of the genus Leishmania ss.
abstract: Two cases of skin lesions similar to those caused by Leishmania parasites have been reported from Martinique. Parasites isolated from these lesions were unlike Leishmania reference strains by isoenzyme analysis and electron microscopy and were assumed to be monoxenous trypanosomatids which normally only infect invertebrates. Both strains have now been retyped by isoenzyme analysis and found to be identical to each other and distantly related to all other Leishmania species. The sequence of the 18S ribosomal RNA gene and partial sequences of the DNA polymerase alpha and RNA polymerase II largest subunit genes were obtained. These sequences indicated that the Martinique parasites clustered with L. enriettii and were basal to all other euleishmania. However, support for both the position basal to all euleishmania and the clustering with L. enriettii was low. The Martinique parasites may cluster with L. (Leishmania) or L. (Viannia) or form a novel clade within the euleishmania either with or without L. enriettii.
authors.affiliation: School of Biological Sciences, University of Liverpool, UK. harry@liv.ac.uk
authors.forename: H
authors.initials: H
authors.lastname: Noyes
authors.forename: F
authors.initials: F
authors.lastname: Pratlong
authors.forename: M
authors.initials: M
authors.lastname: Chance
authors.forename: J
authors.initials: J
authors.lastname: Ellis
authors.forename: G
authors.initials: G
authors.lastname: Lanotte
authors.forename: J P
authors.initials: JP
authors.lastname: Dedet
mesh_terms: D000818:Animals; D004256:DNA Polymerase I / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology* / Q000473:pathology; D008395:Martinique; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D007527:Isoenzymes; D012319:RNA Polymerase II; D004256:DNA Polymerase I