[The effect of calponin and caldesmon in regulation of the gastrointestinal motility during pathophysiological adaptation].
OBJECTIVE
To investigate the expression of calponin (CaP) and caldesmon (CaD) in the gastrointestinal tract and their effect in regulating gastrointestinal motility during physiological and pathological adaptation.


METHODS
Models of chronic gastrointestinal motility hyperfunction in mice were induced by intragastric administration of senna extraction and models of chronic gastrointestinal motility hypofunction in rats were established with carbon tetrachloride induced cirrhosis, CaP and CaD were detected in the gastrointestinal tract of different model groups using SDS-PAGE and Western blot. The color development of Western blots was scanned using densitometric scanning. The relative contents of gastrointestinal CaP and CaD were compared with control animals with different state of gastrointestinal motility.


RESULTS
Animal models of abnormal gastrointestinal motility were established in mice and rats. Densitometric quantification of CaP and CaD blots by CP1 and C98 mAbs showed that normal animal colon contained higher amounts of h1-CaP and CaD. In normal mice and rats, the content of CaP and CaD was successively in this order :colon > stomach > small intestine. The content was reduced in mice of chronic gastrointestinal motility hyperfunction, but the expression of CaP and CaD was promoted in rats with cirrhosis and declined to normal level after treatment with L-NAME.


CONCLUSIONS
There is close relation between expression of CaP and CaD and state of gastrointestinal motility. CaP and CaD may inhibit gastrointestinal motility. These suggest that CaP and CaD may play a role in the regulation of gastrointestinal motility during physiological and pathological adaptation.
Wang
X
X
Institute of Gastroenterology, The Fourth Military Medical University, Xi'an 710033, China.
Wu
K
K
Zhang
Z
Z
Lan
M
M
Jin
J
J
Fan
D
D
D000818:Animals; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000502:physiology*; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis / Q000502:physiology*; D004064:Digestive System / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005765:Gastrointestinal Agents; D005769:Gastrointestinal Motility / Q000187:drug effects*; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D051379:Mice; D008840:Microfilament Proteins; D051381:Rats; D000096985:Calponins
D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D005765:Gastrointestinal Agents; D008840:Microfilament Proteins; D000096985:Calponins