The effect of aloe emodin on the proliferation of a new merkel carcinoma cell line.
A free-floating cell line has been established from a metastatic lesion of a Merkel cell carcinoma (MCC) patient. The cell line was characterized by immunocytochemical reactions with antibodies against the epithelial and neuroendocrine antigens: cytokeratin 20, neuron-specific enolase, chromogranin A, neurofilament protein, synaptophysin, and calcitonin. Karyotype analysis of the MCC cells showed deletion in chromosomes 3 and 7, loss of chromosome 10, and several translocations in other chromosomes. No mutation was detected in the TP53 gene, after analyzing the complete coding region. Growth factors such as basic fibroblast growth factor, transforming growth factor-beta, and nerve and epidermal growth factors had no effect on the proliferation of the cells. The differentiation-inducing agents sodium butyrate and dimethyl sulfoxide, especially the former, markedly inhibited the proliferation of the MCC cells. Aloe emodin, a natural constituent of aloe vera leaves, significantly inhibited the growth of MCC cells. Aloe emodin has been reported to be nontoxic for normal cells but to possess specific toxicity for neuroectodermal tumor cells. Differentiation-inducing agents, and aloe emodin, merit further investigation as potential agents for treating MCC.
Wasserman
Lina
L
Felsenstein Medical Research Center, Sackler Faculty of Medicine, Tel Aviv University, Rabin Medical Center Beilinson Campus, Petah Tikva 49100, Israel. yardenam@clalit.org.ie
Avigad
Smadar
S
Beery
Einat
E
Nordenberg
Jardena
J
Fenig
Eyal
E
D000368:Aged; D000880:Anthraquinones; D000970:Antineoplastic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000032:analysis; D002087:Butyrates / Q000494:pharmacology; D015266:Carcinoma, Merkel Cell / Q000188:drug therapy* / Q000235:genetics / Q000556:secondary; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008297:Male; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D000880:Anthraquinones; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D002087:Butyrates; D006133:Growth Substances; C518327:aloe emodin; D004642:Emodin; D004121:Dimethyl Sulfoxide