High-performance thin layer chromatographic analysis of anti-inflammatory triterpenoids from Boswellia serrata Roxb.
A rapid and simple high-performance thin layer chromatographic (HPTLC) method was developed for the simultaneous quantitative estimation of the biologically active triterpenoids beta-boswellic acid, 3-O-acetyl-beta-boswellic acid, 11-keto-beta-boswellic acid and 3-O-acetyl-11-keto-beta-boswellic acid from the gum resin of Boswellia serrata. The assay combines the isolation and separation of boswellic acid derivatives on silica gel 60F254-HPTLC plates with spot visualisation and scanning at 250 nm. Methanol was found to be the most appropriate solvent for the exhaustive extraction of boswellic acid derivatives.
Krohn
K
K
Universität Paderborn, Fachbereich Chemie und Chemietechnik, 33098 Paderborn, Germany. kk@chemie.uni-paderborn.de
Rao
M S
MS
Raman
N V
NV
Khalilullah
M
M
D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification*; D029743:Boswellia; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
C447943:11-keto-boswellic acid; C459792:3-O-acetyl-beta-boswellic acid; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D014315:Triterpenes; C094432:acetyl-11-ketoboswellic acid; C054625:boswellic acid