BMP-4 and BMP-6 involvement in the osteogenic properties of the HeLa cell line.
The heterotopically induced ossicles are used in our research on bone tissue. The ossicles are formed in the thigh muscle of BALB/c mice under the influence of injected suspension of 3 x 10(6) HeLa cells. We postulate that the mechanism of bone induction is based on the secretion of bone morphogenetic proteins BMP-4 and BMP-6 by the grafted HeLa cells. This was proved by the use of specific immunohistochemical reaction and Western blots of conditioned culture medium. It seems that HeLa cells secrete BMPs continuously into the culture medium, even without contact with the mice muscle tissue, were induction of bone tissue is observed.
Kochanowska
Iwona Ewa
IE
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Wlodarski
Krzysztof
K
Wojtowicz
Andrzej
A
Kinsner
Agnieszka
A
Ostrowski
Kazimierz
K
D000818:Animals; D055415:Bone Morphogenetic Protein 4; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D017077:Culture Media, Conditioned; D006367:HeLa Cells / Q000378:metabolism* / Q000473:pathology* / Q000637:transplantation; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis
C525809:BMP4 protein, human; C525816:BMP6 protein, human; C525810:Bmp4 protein, mouse; C525817:Bmp6 protein, mouse; D055415:Bone Morphogenetic Protein 4; D055418:Bone Morphogenetic Protein 6; D019485:Bone Morphogenetic Proteins; D017077:Culture Media, Conditioned