A synthetic low density lipoprotein particle capable of supporting U937 proliferation in vitro.
A synthetic LDL (sLDL) has been prepared by combining a lipid microemulsion with amphipathic peptides containing the apoprotein B receptor domain. The biological properties of sLDL have been investigated using the U937 in vitro cell proliferation assay. sLDL exhibits a concentration dependent and saturable stimulation of U937 proliferation. By utilizing different amphipathic peptides, variable proliferation is achieved, indicating a specific interaction between sLDL and the U937 LDL receptor are possible. U937 proliferation is reduced by the addition of an anti-LDL receptor antibody, indicating that sLDL is assimilated via the LDL receptor pathway. The behavior of sLDL mimics that of native LDL, and this approach represents a viable technique for the production of an sLDL particle on a large scale for research and general application.
Baillie
G
G
Department of Pharmaceutical Sciences, Strathclyde Institute for Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR, Scotland, UK.
Owens
M D
MD
Halbert
G W
GW
D002455:Cell Division / Q000502:physiology; D004655:Emulsions / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D008223:Lymphoma; D010316:Particle Size; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011973:Receptors, LDL / Q000378:metabolism*; D018110:Receptors, Lipoprotein / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D020298:U937 Cells
D004655:Emulsions; D008055:Lipids; D008077:Lipoproteins, LDL; D010455:Peptides; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; C072413:apolipoprotein B receptor