Effect of Erythrogen on post-thaw recovery of cryopreserved cell suspensions of indica rice (Oryza sativa L.).
This study shows that adding haemoglobin solution (Erythrogen) to post-thaw medium of Indica rice (Oryza sativa L.) cells enhances survival following cryopreservation. Haemoglobin (1:50 - 1:200 v:v) had a beneficial effect on post-thaw viability and subsequent cell growth. A key finding was that the successful recovery from cryopreservation of cell suspensions of the Indica rice cvs. BR26 and Pajam, and their re-establishment in AA2 medium, reflected a requirement for such supplementation of the post-thaw recovery medium with Erythrogen.
Al-Forkan
M
M
Plant Sciences Division, School of Biosciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
Anthony
P
P
Power
J B
JB
Davey
M R
MR
Lowe
K C
KC
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology*; D012275:Oryza / Q000166:cytology* / Q000254:growth & development
D003451:Cryoprotective Agents; D017077:Culture Media, Conditioned; D006454:Hemoglobins; C446559:erythrogen