Laser activated fluorescence measurements and morphological features: an in vivo study of clearance time of fluorescein isothiocyanate tagged cell markers.
Fourteen BALB/c mice were divided into two groups. One group served as the control and the second group was injected with a squamous cell carcinoma cell line to the tongue. After tumor development (1-4 weeks), mice were injected with a FITC conjugated CD3 marker to their tongues. Immediately after the marker injection, the clearance of the marker was measured using a laser spectroscopy system. The markers were excited by an argon laser at 488 nm and the fluorescence signal was measured as a function of time. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. Analysis of clearance times revealed a second order exponential decay for both groups with a slower pace of signal clearance for the sick mice.
Gannot
I
I
Tel-Aviv University, Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv 69978, Israel. gannot@eng.tau.ac.il
Gannot
G
G
Garashi
A
A
Gandjbakhche
A
A
Buchner
A
A
Keisari
Y
Y
D000818:Animals; D000906:Antibodies; D014408:Biomarkers, Tumor; D001706:Biopsy; D017252:CD3 Complex / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D016650:Fluorescein-5-isothiocyanate / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007834:Lasers; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D012016:Reference Values; D014062:Tongue Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000906:Antibodies; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate