title: Inhibitory effect of a Korean traditional medicine, Honghwain-Jahage (water extracts of Carthamus tinctorius L. seed and Hominis placenta) on interleukin-1-mediated bone resorption.
abstract: A Korean herbal formulation, Honghwain-Jahage (HJ), which is comprised of a herb of Carthamus tinctorius L. seed and Hominis Placenta, was investigated for inhibiting effects on IL-1 beta-stimulated bone resorption in the fetal mouse bone culture system. Results of in vitro cytotoxicities showed that HJ extracts have no any cytotoxicities in concentrations of 1-200 microg/ml on the cultured osteoblast cells derived from mouse calvarial bone explants. Cell viability was not significantly affected by treatment with the indicated concentration of the extracts. The HJ extracts were shown to have inhibitory effects against the synthesis of PGE(2). We also examined the effect of the pretreatment with various concentrations of the HJ extracts then treated by the PGE(2)-induction agents. Pretreatment of the HJ extracts for 1 h, which by itself had little effect on cell survival, reduced the synthesis of PGE(2). Furthermore, the HJ extracts were shown to have protective effects against plasminogen dependent fibrinolysis induced by IL-1 beta. Pretreatment of the HJ extracts for 1 h did not enhance the plasminogen dependent fibrinolysis. Finally, co-treatment of HJ with calcitonin showed significant inhibitory activity on the IL-1 beta-stimulated bone resorption. From these results, it was found that HJ extracts inhibited IL-1 beta-induced bone resorption.
authors.affiliation: Department of Biochemistry, Molecular Biology and Gynecology, College of Oriental Medicine, Dong-Guk University, Sukjang-Dong #707, Kyung-Pook 780-714, Kyung-Ju City, South Korea.
authors.forename: Hee-Tak
authors.initials: HT
authors.lastname: Hong
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Tae-Kyun
authors.initials: TK
authors.lastname: Lee
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
authors.forename: Young-Guk
authors.initials: YG
authors.lastname: Park
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy / Q000378:metabolism*; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D005333:Fetus; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D007723:Korea; D008518:Medicine, East Asian Traditional; D051379:Mice; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology; D028321:Plant Preparations / Q000494:pharmacology* / Q000627:therapeutic use; D011257:Pregnancy Proteins / Q000494:pharmacology*; D012639:Seeds
chemical_list: D007375:Interleukin-1; D010936:Plant Extracts; D028321:Plant Preparations; D011257:Pregnancy Proteins; D015232:Dinoprostone