title: kuzbanian-mediated cleavage of Drosophila Notch.
abstract: Loss of Kuzbanian, a member of the ADAM family of metalloproteases, produces neurogenic phenotypes in Drosophila. It has been suggested that this results from a requirement for kuzbanian-mediated cleavage of the Notch ligand Delta. Using transgenic Drosophila expressing transmembrane Notch proteins, we show that kuzbanian, independent of any role in Delta processing, is required for the cleavage of Notch. We show that Kuzbanian can physically associate with Notch and that removal of kuzbanian activity by RNA-mediated interference in Drosophila tissue culture cells eliminates processing of ligand-independent transmembrane Notch molecules. Our data suggest that in Drosophila, kuzbanian can mediate S2 cleavage of Notch.
authors.affiliation: Laboratory of Genetics, The Rockefeller University, New York, NY 10021, USA.
authors.forename: Toby
authors.initials: T
authors.lastname: Lieber
authors.forename: Simon
authors.initials: S
authors.lastname: Kidd
authors.forename: Michael W
authors.initials: MW
authors.lastname: Young
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D017931:DNA Primers; D019483:Disintegrins / Q000378:metabolism*; D004330:Drosophila / Q000196:embryology; D029721:Drosophila Proteins; D006868:Hydrolysis; D008565:Membrane Proteins / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000378:metabolism*; D010641:Phenotype; D011233:Precipitin Tests; D012313:RNA / Q000378:metabolism; D051880:Receptors, Notch
chemical_list: D017931:DNA Primers; D019483:Disintegrins; D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D012313:RNA; C100953:KUZ protein, Drosophila; D008666:Metalloendopeptidases