Deletion of the two-component histidine kinase gene (CHK1) of Candida albicans contributes to enhanced growth inhibition and killing by human neutrophils in vitro.
We have observed that human neutrophils (polymorphonuclear leukocytes [PMNs]) have an increased growth-inhibitory and killing effect on a strain of Candida albicans with a deletion of CHK1, a gene encoding a putative histidine kinase. The PMN effect was not due to increased phagocytosis of the null strain. This observation may partially explain the reduced virulence in a hematogenously disseminated murine model of candidiasis.
Torosantucci
Antonella
A
Istituto Superiore di Sanita, Laboratory of Bacteriology and Mycology, Viale Regina Elena 299, Rome 00196, Italy.
Chiani
Paola
P
De Bernardis
Flavia
F
Cassone
Antonio
A
Calera
Jose Antonio
JA
Calderone
Richard
R
D002176:Candida albicans / Q000201:enzymology* / Q000276:immunology; D002478:Cells, Cultured; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D000071677:Histidine Kinase; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000276:immunology*; D011494:Protein Kinases / Q000276:immunology*
D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011494:Protein Kinases; D000071677:Histidine Kinase