Expression and distribution of calcitonin receptor-like receptor in human hairy skin.
Calcitonin gene-related peptide and adrenomedullin exert potent effects in skin but their cellular targets are unknown. This study aimed to identify the cellular location of calcitonin receptor-like receptor (CRLR) which is pharmacologically identical to CGRP receptor-1, a putative molecular target of CGRP and adrenomedullin. RT-PCR analysis of human hairy skin revealed the presence of CRLR mRNA and immunohistochemical analysis, employing a previously characterized polyclonal antibody raised to CRLR, provided novel evidence of the cellular distribution of CRLR. Extensive and specific CRLR-immunostaining was detected in arteriolar smooth muscle and venular endothelium and is consistent with CGRP's putative role in neurogenic inflammation. Novel targets for CGRP and/or adrenomedullin were identified, including capillary endothelium, hair follicles and sweat glands.
Hagner
S
S
Institute of Physiology, Philipps University of Marburg, Marburg, Germany.
Haberberger
R V
RV
Overkamp
D
D
Hoffmann
R
R
Voigt
K H
KH
McGregor
G P
GP
D053607:Adrenomedullin; D001158:Arteries / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D058286:Calcitonin Receptor-Like Protein; D002196:Capillaries / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006197:Hair / Q000378:metabolism*; D018859:Hair Follicle / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009130:Muscle, Smooth / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018003:Receptors, Calcitonin / Q000096:biosynthesis* / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D013545:Sweat Glands / Q000378:metabolism
C545578:CALCRL protein, human; D058286:Calcitonin Receptor-Like Protein; D010455:Peptides; D012333:RNA, Messenger; D018003:Receptors, Calcitonin; D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide