Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng-based pharmaceuticals using a diol column.
A reversed-phase high-performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb(1), Rb(2), Rc, Rd, Rg(1), Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 x 4 mm Lichrospher, 5 microm, 100 A diol column with detection at 203 nm. The method was validated over the range of 2.5-20 ng/microL using a 20 microL sample volume. The average accuracy at five concentrations was 90-100%, and the within-day and between-day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively.
Bonfill
Mercè
M
Universitat de Barcelona, Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Avda Diagonal no. 643, 08028 Barcelona, Catalonia, Spain. bonfill@farmacia.far.ub.es
Casals
Isidre
I
Palazón
Javier
J
Mallol
Anna
A
Morales
Carmen
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides; D005894:Panax / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D036145:Ginsenosides; D004364:Pharmaceutical Preparations; D012503:Saponins