Fv1-like restriction of N-tropic replication-competent murine leukaemia viruses in mCAT-1-expressing human cells.
To study the replication of murine leukaemia viruses in human cells we have used full-length as well as EGFP-tagged ecotropic viruses in combination with mCAT-1-expressing human cells. We present results showing that N-tropic murine leukaemia viruses are restricted in both infection and replication in such cells while B-tropic viruses, modified at capsid position 110, escape restriction. These results support a recently reported Fv1-like restriction in mammalian cells. We extend the analysis of Fv1-like restriction by demonstrating that NB-tropic viruses also escape restriction and human mCAT-1-expressing cells are thus similar to murine Fv1(b) cells with respect to infection though the ecotropic receptor pathway.
Aagaard
Lars
L
Department of Molecular and Structural Biology1, Department of Medical Microbiology and Immunology2, C. F. Moellers Allé, Bldg 130, DK-8000 Aarhus, University of Aarhus, Denmark.
Mikkelsen
Jacob Giehm
JG
Department of Molecular and Structural Biology1, Department of Medical Microbiology and Immunology2, C. F. Moellers Allé, Bldg 130, DK-8000 Aarhus, University of Aarhus, Denmark.
Warming
Søren
S
Department of Molecular and Structural Biology1, Department of Medical Microbiology and Immunology2, C. F. Moellers Allé, Bldg 130, DK-8000 Aarhus, University of Aarhus, Denmark.
Duch
Mogens
M
Department of Molecular and Structural Biology1, Department of Medical Microbiology and Immunology2, C. F. Moellers Allé, Bldg 130, DK-8000 Aarhus, University of Aarhus, Denmark.
Pedersen
Finn Skou
FS
Department of Molecular and Structural Biology1, Department of Medical Microbiology and Immunology2, C. F. Moellers Allé, Bldg 130, DK-8000 Aarhus, University of Aarhus, Denmark.
D000818:Animals; D027201:Cationic Amino Acid Transporter 1 / Q000378:metabolism; D002460:Cell Line; D049452:Green Fluorescent Proteins; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D007942:Leukemia, Experimental / Q000821:virology; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D011506:Proteins / Q000235:genetics*; D012192:Retroviridae Infections / Q000821:virology*; D014412:Tumor Virus Infections / Q000821:virology*; D014779:Virus Replication
D027201:Cationic Amino Acid Transporter 1; C103189:Fv1 protein, mouse; D008164:Luminescent Proteins; D011506:Proteins; C420826:Slc7a1 protein, mouse; D049452:Green Fluorescent Proteins