[The chemotactic response of gingival fibroblasts and periodontal ligament cells to interleukin-8].
OBJECTIVE
To evaluate the chemotactic response of gingival fibroblasts and periodontal ligament cells to interleukin-8.


METHODS
The passage 6 cultured gingival fibroblasts and periodontal ligament cells (2.5 x 10(8) cell/L, respective) were placed in the upper compartment of the Transwell cell culture chamber. The lower compartment contained IL-8 with the concentrations of 10(-10), 10(-9), 10(-8), 10(-7) mol/L, respectively. Incubation was at 37 degrees C in an atmosphere of 95% air and 5% CO2 for 24 h. The number of migrated cells were counted under high power fields.


RESULTS
IL-8 at concentrations ranged from 10(-9)-10(-7) mol/L promoted the chemotactic movement of gingival fibroblasts in a dose-dependent manner. However, at the same IL-8 concentrations the chemotactic movement of periodontal ligament cells was not elevated.


CONCLUSIONS
IL-8 may play its role in regulating chemotaxis of gingival fibroblast.
Luan
Q
Q
Department of Periodontology, Peking University School of Stomatology, Beijing 100081, China.
Toshiaki
S
S
Katsunori
M
M
D002633:Chemotaxis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*
D016209:Interleukin-8