Vanilloid receptor type 1-immunoreactivity is expressed by intrinsic afferent neurones in the guinea-pig myenteric plexus.
The enteric sensory nervous system consists of extrinsic and intrinsic components. The cellular distribution of vanilloid receptor type 1 (VR1) and its relation to the intrinsic sensory neurones were studied in myenteric plexus-longitudinal muscle preparations of rat ileum and guinea-pig ileum and colon. VR1-immunoreactivity was localized on fine fibres and expressed by ganglionic cells. In the guinea-pig myenteric plexus, a proportion of VR1-immunoreactive cells co-localized with calbindin, a marker for intrinsic afferent neurones. Reverse transcription-polymerase chain reaction with rat VR1-specific primers detected VR1 mRNA in rat but not in guinea-pig preparations. We conclude that VR1 is expressed on fibres and by myenteric neurones. In the guinea-pig, VR1 is expressed by intrinsic afferent neurones but its mRNA may differ from the rat sequence in the region of the primers.
Anavi-Goffer
Sharon
S
Department of Biomedical Science, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, UK.
McKay
Neil G
NG
Ashford
Michael L J
ML
Coutts
Angela A
AA
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D064026:Calbindins; D048429:Cell Size / Q000502:physiology; D003106:Colon / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000166:cytology / Q000294:innervation / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000294:innervation*; D008297:Male; D018613:Microscopy, Confocal; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism*; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D009619:Nociceptors / Q000166:cytology / Q000378:metabolism*; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D017833:Visceral Afferents / Q000166:cytology / Q000378:metabolism*
D064026:Calbindins; D012333:RNA, Messenger; D011955:Receptors, Drug; D064030:S100 Calcium Binding Protein G