Temperature-responsive chromatography using poly(N-isopropylacrylamide) hydrogel-modified silica.
Poly(N-isopropylacrylamide) (PNIPAAm) has the sharpest phase transition of the class of thermo-sensitive N-alkyl acrylamide polymers. We developed a new method of HPLC using packing materials modified with cross-linked poly(N-isopropylacrylamide) (PNIPAAm) hydrogel. A temperature-responsive surface was prepared by polymerization of NIPAAm in the presence of a cross-linker on the silica support. The surface properties and functions of the stationary phases change in response to the external temperature. Therefore it easily changes the interaction of a solute with the surface with a constant aqueous mobile phase. A temperature-responsive elution behavior was observed on the separation of steroids and PTH-amino acids. The method is expected to be applicable to separation in the pharmaceutical and biomedical fields.
Kanazawa
Hideko
H
Kyoritsu College of Pharmacy,Minato, Tokyo, Japan.
Sunamoto
Tatsuo
T
Ayano
Eri
E
Matsushima
Yoshikazu
Y
Kikuchi
Akihiko
A
Okano
Teruo
T
D000178:Acrylamides; D000179:Acrylates; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D011108:Polymers; D013696:Temperature; D013816:Thermodynamics
D000178:Acrylamides; D000179:Acrylates; D007202:Indicators and Reagents; C090629:N-isopropylacrylamide-acrylic acid copolymer; D011108:Polymers