[The application of Apo-1/Fas to evaluate apoptosis of myocardial cells in patients with congestive heart failure].
OBJECTIVE
To study the changes of serum level of Apo-1/Fas in patients with congestive heart failure (CHF) and evaluate apoptosis of failing myocardial cells.


METHODS
Strepavidin-Biotin ELISA was used to determine serum level of Apo-1/Fas, interleukin-6 (IL-6) and tumor necrosis factor (TNF alpha) in 60 patients with CHF. Cardiac ejection fraction (EF) of the patients were measured by acusson 128XP/10 echocardiograph.


RESULTS
Serum levels of Apo-1/Fas and TNF alpha in class III and IV patients with CHF were significantly higher than those in class I and II (P < 0.01). Serum levels of IL-6 in all the patients were obviously higher than those in controls (P < 0.05 and 0.01) and the levels in class III and IV patients were significantly higher than those in class I and II (P < 0.05). Serum levels of Apo-1/Fas in patients with EF < 55 percent were higher than in those with EF > 55 percent (P < 0.05).


CONCLUSIONS
Serum level of Apo-1/Fas in patients with CHF reflects a state of apoptosis in failing myocardial cells. IL-6 and TNF alpha have important effects on immune regulation of myocardial cell apoptosis.
Li
Z
Z
Department of Cardiovascular, Zhujiang Hospital, First Military Medical University, Guangzhou 510282.
Jiao
B
B
Qian
H
H
Qian
X
X
Mo
B
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006333:Heart Failure / Q000276:immunology / Q000473:pathology*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D019014:fas Receptor / Q000097:blood*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor