Protective effect of wogonin on endotoxin-induced lethal shock in D-galactosamine-sensitized mice.
The effects of wogonin, a major flavonoid from Scutellaria baicalensis Georgi, on lipopolysaccharide (LPS)-induced lethal shock in mice was investigated. Wogonin pretreatment prevented the lethal shock in mice injected with D-galactosamine (D-GalN) and LPS, but not in mice injected with a high dose of LPS. Wogonin definitely inhibited the hepatic injury in mice injected with D-GalN, and LPS and reduced the level of circulating tumor necrosis factor (TNF)-alpha. The reduction was more marked in mice injected with D-GalN and LPS compared with that in mice injected with a high dose of LPS. Wogonin pretreatment did not inhibit the lipid peroxidation in mice receiving either D-GalN and LPS or a high dose of LPS. Wogonin inhibited the in vitro production of TNF-alpha and nitric oxide in LPS-stimulated RAW 264.7 cells. The mechanism of the protective effect of wogonin on the lethal shock in mice injected with D-GalN and LPS is discussed.
Van Dien
M
M
Department of Microbiology and Immunology, Research Center for Infectious Disease, Aichi Medical University School of Medicine, Nagakute, Japan.
Takahashi
K
K
Mu
M M
MM
Koide
N
N
Sugiyama
T
T
Mori
I
I
Yoshida
T
T
Yokochi
T
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D005260:Female; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use*; D005688:Galactosamine; D008054:Lipid Peroxides / Q000378:metabolism; D008070:Lipopolysaccharides; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D012772:Shock, Septic / Q000139:chemically induced / Q000401:mortality / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; D008054:Lipid Peroxides; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D005688:Galactosamine; C085514:wogonin