title: Concentration of chosen oxycholesterols in plasma of pregnant women with pregnancy-induced hypertension.
abstract: Solid-phase extraction (SPE) was applied for isolation of oxycholesterols from plasma lipid extract from pregnant women with hypertension and from a control group. Separation of oxycholesterols fraction was performed in an SD II horizontal chamber (Chromdes, Poland) using silica gel and octadecyl RPC18 silica gel TLC plates (Merck and Machery Nagel). Visualization was carried out under UV light after Liebermann-Burchard reaction specific for cholesterol and its derivatives. The oxycholesterols (5-cholestene-3beta-ol-7-one, sum of 5-cholestene-3beta, 7beta-diol and 5-cholestene-3beta, 7alpha-diol and sum of 5alpha,6alpha-epoxycholestan-3beta-ol and 5beta, 6beta-epoxycholestan-3beta-ol) were quantified by chromatograms scanning in reflectance and fluorescence mode using a CS 9301 densitometer (Shimadzu). The total concentration of the investigated oxycholesterols in the plasma of pregnant women was up to 5000 ng/mL and was statistically significantly higher in women with pregnancy induced hypertension (PIH).
authors.affiliation: Department of Obstetrics and Gynaecology, Medical University of Silesia, Faculty of Medicine, Zabrze, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Bodzek
authors.forename: Beata
authors.initials: B
authors.lastname: Janoszka
authors.forename: Tomasz
authors.initials: T
authors.lastname: Wielkoszyński
authors.forename: Danuta
authors.initials: D
authors.lastname: Bodzek
authors.forename: Aleksander
authors.initials: A
authors.lastname: Sieroń
mesh_terms: D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood* / Q000737:chemistry / Q000145:classification; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000097:blood*; D014466:Ultraviolet Rays
chemical_list: D002784:Cholesterol