title: Synovial fluid studies in navicular disease.
abstract: The purpose of this study was to investigate biochemical changes in synovial fluid in navicular disease, and to establish if synovial fluid from the distal interphalangeal joint (DIP) could be used diagnostically to assess alterations in the synovial fluid of the navicular bursa. Cartilage oligomeric matrix protein (COMP), total glycosaminoglycans (GAG), hyaluronan (HA), metalloproteinases 2 and -9 (MMP-2 and MMP-9) and total protein (TP) levels were determined in synovial fluids obtained from 18 navicular bursae and 35 DIP -joints from animals suffering from navicular disease, and the same synovial structures in 16 joints of horses with no evidence of abnormalities involving the foot. To avoid dilution effects, GAG/COMP, HA/COMP, MMP-2/ COMP and MMP-9/COMP ratios were also calculated for different synovial cavities. There was a good correlation, for COMP, GAG, HA, MMP-2 and TP levels, between synovial fluid from the navicular bursa and fluid from the DIP -joint in healthy animals. However, in animals with navicular disease, only COMP levels showed no difference between the navicular bursal fluid and the DIP-joint fluid concentration. Thus, enabling the use of COMP to standardise other biochemical concentration measurements from the synovial joint fluids. In horses with navicular disease, there was a significantly lower absolute concentration of GAG, and a significantly lower GAG/COMP ratio, in the synovial fluid of the navicular bursa and the DIP-joint compared to synovial fluid from the same joints from healthy horses. In contrast, the absolute HA concentration and HA/ COMP, MMP-2/COMP and MMP-9/COMP ratios were higher in synovial fluid from the DIP-joint of horses with navicular disease, and MMP-2 and MMP-9 relative activity levels and MMP-2/COMP and MMP-9/ COMP ratios were increased in fluid from navicular bursae in horses with navicular disease when compared to a control group.
authors.affiliation: Farm Animal and Equine Medicine and Surgery, Royal Veterinary College, University of London, Hawkshead Lane, North Mymms, Hatfield, Herts, AL 9 7 TA, UK. minna@viitanen.freeserve.co.uk
authors.forename: M
authors.initials: M
authors.lastname: Viitanen
authors.forename: J
authors.initials: J
authors.lastname: Bird
authors.forename: O
authors.initials: O
authors.lastname: Makela
authors.forename: M
authors.initials: M
authors.lastname: Schramme
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: R M
authors.initials: RM
authors.lastname: Tulamo
authors.forename: S
authors.initials: S
authors.lastname: May
mesh_terms: D000818:Animals; D002061:Bursa, Synovial / Q000503:physiopathology*; D005260:Female; D005534:Foot Diseases / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D006724:Hoof and Claw; D006734:Horse Diseases / Q000175:diagnosis / Q000503:physiopathology*; D006736:Horses; D007596:Joints / Q000737:chemistry / Q000502:physiology; D007794:Lameness, Animal / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology; D008297:Male; D011506:Proteins / Q000032:analysis; D013582:Synovial Fluid / Q000737:chemistry* / Q000502:physiology
chemical_list: D011506:Proteins