Flow cytometric and immunophenotypic evaluation of acute lymphocytic leukemia in dog bone marrow.
Monoclonal antibodies provide powerful tools for detection of lineage-specific markers on hematopoietic cells. We used the combination of cell morphology, cytochemistry, flow cytometric scatter plot analysis, and labeling of cells with 6 monoclonal antibodies to detect and subclassify lymphocytic leukemia in bone marrow from 5 dogs. Antibodies included anti-CD18 (a panleukocyte marker), anti-MHC class II (detects most B and T lymphocytes and monocyte/macrophages), anti-Thy-1 (a pan-T-lymphocyte and monocyte/macrophage marker), anti-CD3 (a pan-T-lymphocyte marker), anti-CD21 (a B-lymphocyte marker), and anti-CD14 (a monocyte/macrophage marker). Of the 5 dogs evaluated, 2 were categorized as acute T-cell prolymphocytic leukemia, 2 as acute non-T, non-B lymphoblastic leukemia, and 1 as acute B-cell lymphoblastic leukemia. Results of this study indicate marked variation in the morphology and immunophenotype of canine lymphocytic leukemia.
Weiss
D J
DJ
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA. weiss005@tc.umn.edu
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000473:pathology; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary*; D016130:Immunophenotyping / Q000662:veterinary*; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*
D000911:Antibodies, Monoclonal