[Vascular lesion and its mechanisms in spleen under statement of portal hypertension].
OBJECTIVE
To investigate the vascular lesion and its mechanisms in spleen in patients with portal hypertension.


METHODS
28 specimens of spleen from portal hypertension patients and 10 from normal controls were stained by HE and Masson Trichrome to study their vascular pathological lesions. The proteins of PCNA, and Flt-1 were analyzed by immunohistochemistry. The mRNAs of VEGF and bFGF were also identified with in situ hybridization.


RESULTS
Various vascular lesions occurred, such as sclerosis endangitis fibrosis and endothelial cell damage. The proteins of PCNA, and Flt-1 were intensively immunostained in portal hypertension spleens compared with normal controls. The mRNAs of VEGF and bFGF were highly expressed, but weakly in controls.


CONCLUSIONS
(1) Vascular lesion of spleen is one part of portal hypertensive vascular lesions. (2) The mechanisms of these lesions are due to the changes of microcirculation and hemodynamics, immune disorders, increasing growth factors such as VEGF, Flt-1 and bFGF both at protein and transcription levels.
Shi
B
B
Department of Surgery Tongji Hospital, Tongji Medical University, Wuhan 430030, China.
Yang
Z
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D002196:Capillaries / Q000473:pathology*; D016228:Endothelial Growth Factors / Q000235:genetics; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics; D006801:Humans; D006975:Hypertension, Portal / Q000378:metabolism / Q000473:pathology*; D008222:Lymphokines / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D012333:RNA, Messenger; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis; D013154:Spleen / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D013157:Splenic Artery / Q000473:pathology*; D013162:Splenic Vein / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D020794:Receptor Protein-Tyrosine Kinases; D040281:Vascular Endothelial Growth Factor Receptor-1