title: Nociceptin/orphanin FQ tissue concentration in the rat brain. Effects of repeated ethanol administration at various post-treatment intervals.
abstract: The aim of this study was to study short- and long-term effects of repeated ethanol administration on nociceptin/orphanin FQ (N/OFQ) tissue concentrations in rat brain with radioimmunoassay. Animals were given either ethanol (intraperitoneal) or saline for 13 consecutive days. N/OFQ levels were examined at 30 min, 5 days and 21 days after the last dose on day 13. Ethanol-treated rats had significantly decreased N/OFQ tissue concentration in the hippocampus at 30 min after the last dose. N/OFQ levels were decreased in the cingulate cortex at 5 days after cessation of ethanol administration whereas no significant changes were found at 21 days. There were no significant changes in N/OFQ tissue concentrations at any time point studied in the mesolimbic dopamine (DA) system, a brain area associated with ethanol-induced activation. However, the results indicate that repeated ethanol administration may induce short- and long-term changes in N/OFQ tissue concentrations in other brain regions innervated with dopaminergic neurons.
authors.affiliation: Department of Clinical Neuroscience, Karolinska Institutet, Sweden.
authors.forename: Sara
authors.initials: S
authors.lastname: Lindholm
authors.forename: Karolina
authors.initials: K
authors.lastname: Ploj
authors.forename: Johan
authors.initials: J
authors.lastname: Franck
authors.forename: Ingrid
authors.initials: I
authors.lastname: Nylander
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D002492:Central Nervous System Depressants / Q000008:administration & dosage*; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage*; D008297:Male; D018847:Opioid Peptides / Q000378:metabolism*; D009928:Organ Specificity / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000378:metabolism*; D013997:Time Factors; D000094942:Nociceptin Receptor; D000097629:Nociceptin
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722285:Oprl1 protein, rat