Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector.
Here we report the production of transgenic pigs that express enhanced green fluorescent protein (eGFP). Porcine oocytes were matured in vitro in a serum-free, chemically defined maturation medium, subsequently infected with a replication deficient pseudotyped retrovirus, fertilized and cultured in vitro before being transferred to a recipient female. Two litters were born from these embryo transfers; one pig from each litter was identified as transgenic and both expressed eGFP. From a tool in basic research to direct applications in production agriculture, domestic livestock capable of expressing foreign genes have many scientific applications.
Cabot
R A
RA
Department of Animal Sciences, University of Missouri-Columbia, 65211, USA.
Kühholzer
B
B
Chan
A W
AW
Lai
L
L
Park
K W
KW
Chong
K Y
KY
Schatten
G
G
Murphy
C N
CN
Abeydeera
L R
LR
Day
B N
BN
Prather
R S
RS
D000383:Agriculture; D000818:Animals; D030801:Animals, Genetically Modified; D015415:Biomarkers / Q000032:analysis; D004624:Embryo Transfer; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000096:biosynthesis; D008297:Male; D009865:Oocytes; D012190:Retroviridae; D013552:Swine / Q000235:genetics*
D015415:Biomarkers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins