[Linkage analysis of candidate genes for glucose and lipid metabolism with essential hypertension].
OBJECTIVE
To identify the genetic loci responsible for susceptibility to essential hypertension (EH) in chromosomal regions containing candidate genes involved in the regulation of glucose and lipid metabolism.


METHODS
Linkage analysis of EH were performed in 95 Chinese nuclear families with 477 subjects using the technique of fluorescence-based gene scan with microsatellite markers. Markers were selected in 11 chromosomal regions containing 18 candidate genes regulating blood glucose and lipid metabolism. The two-locus, non-parametric linkage analysis (NPL), maximum LOD score and transmission/disequilibrium test (TDT) with GENEHUNTER program were used in this study.


RESULTS
No significant linkages were found by NPL and LOD score analysis (P > 0.05 or LOD score < 1) at all loci. The results of TDT showed significant linkage with EH at D8S261 and D11S1347 (chi(2) = 11.92, P < 0.001 and chi(2) = 7.37, P < 0.01, respectively). Near these loci, there were a few candidate genes including lipoprotein lipase (LPL) and a cluster of apolipoprotein (apoA1-C3-A4).


CONCLUSIONS
TDT suggests significant linkage with EH at D8S261 and D11S1347. Whether the genes located at or near the two loci, such as LPL and apoA1-C3-A4, are susceptibility genes of EH need to be verified.
Chu
S
S
Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Zhu
D
D
Xiong
M
M
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged
D001786:Blood Glucose; D008055:Lipids