title: The chicken lysozyme chromatin domain contains a second, widely expressed gene.
abstract: The chicken lysozyme (cLys) locus has been shown to contain all of the cis-elements necessary for position-independent and tissue-specific expression entirely within a 24-kb region defined by general DNase I sensitivity and flanked by matrix attachment regions. As such, it has been viewed as an example of a functional chromatin domain, which is structurally and functionally isolated from neighbouring chromatin. We report here the identification and characterisation of the chicken glioma-amplified sequence (cGas41) locus, which though widely expressed, is contained entirely within the lysozyme chromatin domain. The cGas41 transcript encodes a putative transcription factor, starts 207 bp downstream of the cLys polyadenylation site and is preceded by a CpG island with proposed dual promoter/origin function. The location and differential expression of cGas41 compels re-evaluation of the accumulated literature on the lysozyme domain, and represents an example of two unrelated, differentially expressed vertebrate genes coexisting in the same functional chromatin domain.
authors.affiliation: Department of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
authors.forename: Suyinn
authors.initials: S
authors.lastname: Chong
authors.forename: Arthur D
authors.initials: AD
authors.lastname: Riggs
authors.forename: Constanze
authors.initials: C
authors.lastname: Bonifer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens / Q000235:genetics*; D002843:Chromatin / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D020869:Gene Expression Profiling; D023061:Gene Order / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics*; D005910:Glioma / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics
chemical_list: D030161:Avian Proteins; D002843:Chromatin; D012333:RNA, Messenger; D014157:Transcription Factors; D009113:Muramidase