title: Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells.
abstract: Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O(2)(-)) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O(2)(-) in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O(2)(-) generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O(2)(-) generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Ha Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohigashi
mesh_terms: D000493:Allopurinol / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens; D002454:Cell Differentiation; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010703:Phorbol Esters; D010936:Plant Extracts / Q000627:therapeutic use*; D013481:Superoxides; D013755:Tetradecanoylphorbol Acetate; D014407:Tumor Cells, Cultured; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D004791:Enzyme Inhibitors; D010703:Phorbol Esters; D010936:Plant Extracts; D013481:Superoxides; D000493:Allopurinol; D014969:Xanthine Oxidase; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen