Gene expression profiling in AGS cells stimulated with Helicobacter pylori isogenic strains (cagA positive or cagA negative).
To study host response to CagA, human gastric cancer cell line AGS was infected with a Helicobacter pylori type I wild-type or isogenic cagA-negative mutant. Differentially expressed genes were identified using cDNA array technology. By Northern blotting, downregulation of focal adhesion kinase and upregulation of LIM kinase mRNA in the presence of CagA were clearly verified. Furthermore, upregulation of LIM kinase, macrophage inflammatory protein-2, c-myc, and bone morphogenetic protein-1 and downregulation of transcription factor Y-box binding protein-1 and focal adhesion kinase mRNA in response to H. pylori type I infection compared to the uninfected control could be shown by Northern blotting. Hence, these findings identified new targets for further functional studies on H. pylori-associated pathogenesis.
Bach
Susanne
S
Department of Clinical Microbiology, Hygiene Institute, University of Vienna, A-1090 Vienna, Austria.
Makristathis
Athanasios
A
Rotter
Manfred
M
Hirschl
Alexander M
AM
D000942:Antigens, Bacterial / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics; D054426:Chemokine CXCL2; D018925:Chemokines / Q000235:genetics; D004268:DNA-Binding Proteins; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D054461:Lim Kinases; D008666:Metalloendopeptidases / Q000235:genetics; D016296:Mutagenesis; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D014157:Transcription Factors; D014407:Tumor Cells, Cultured; D051840:Y-Box-Binding Protein 1
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D019485:Bone Morphogenetic Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; C080866:cagA protein, Helicobacter pylori; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human; C515892:LIMK1 protein, human; D054461:Lim Kinases; C515893:Limk1 protein, mouse; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; C525707:Bmp1 protein, mouse; D055395:Bone Morphogenetic Protein 1