title: Investigation of titanium leak to bone tissue surrounding dental titanium implant: electron microscopic findings and analysis by electron diffraction.
abstract: This study investigated the tissue response associated with dental titanium implants. The mandibular third and fourth premolars and first molar of three adult beagle dogs were extracted bilaterally. Healing was then allowed for 3 months. Six titanium implants were placed in the mandibles of a dog. Three weeks after the implantation, mandibular sections containing the implants were retrieved with the use of a bone saw and investigated by light and electron microscopy, X-ray microanalyzer, and electron diffraction. Scanning electron microscopic observation showed titanium particles on the implant-bone interface, and investigation by microanalyzer revealed titanium not only on the implant-bone interface but also in the bone tissue. Transmission electron microscopic observation and investigation by electron diffraction showed titanium in the bone matrix and cells other than macrophages. In this study, titanium particles from the dental implant were recognized morphologically in the surrounding bone tissue. Thus, study of the influence of titanium particles on the human body is needed.
authors.affiliation: Department of Oral Surgery, Sapporo Medical University School of Medicine, South 1, West 16, Chuou-ku, Sapporo 060-0061, Japan. ntanaka@sapmed.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Ichinose
authors.forename: Y
authors.initials: Y
authors.lastname: Kimijima
authors.forename: M
authors.initials: M
authors.lastname: Mimura
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D015921:Dental Implants / Q000009:adverse effects*; D004285:Dogs; D004577:Electron Probe Microanalysis; D008854:Microscopy, Electron; D014025:Titanium / Q000032:analysis*
chemical_list: D015921:Dental Implants; D014025:Titanium