Isolation and identification of a new impurity in lorazepam substance.
A new impurity in lorazepam (1) was UV-detected during the HPLC-analysis of compound 1, batch scale. After the preparative chromatography on silica gel this impurity was isolated. According to IR, NMR, MS and elemental analysis the new impurity was identified as 7-chloro-5-(2-chlorophenyl)-2,3- dioxo-2,3,4,5-tetrahydro-1H-1,4-benzodiazepine (2).
Cepanec
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Department for Development of Chemical Synthesis, Belupo Pharmaceuticals & Cosmetics, Zagreb, Croatia. belupo-razvoj-sinteze@belupo.tel.hr
Mikuldas
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Litvić
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Vukusić
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D014151:Anti-Anxiety Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D008140:Lorazepam / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared
D014151:Anti-Anxiety Agents; D008140:Lorazepam