Novel non-Mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae.
Two cytoplasmically inherited determinants related by their manifestation to the control of translation accuracy were previously described in yeast. Cells carrying one of them, [PSI(+)], display a nonsense suppressor phenotype and contain a prion form of the Sup35 protein. Another element, [PIN(+)], determines the probability of de novo generation of [PSI(+)] and results from a prion form of several proteins, which can be functionally unrelated to Sup35p. Here we describe a novel nonchromosomal determinant related to the SUP35 gene. This determinant, designated [ISP(+)], was identified as an antisuppressor of certain sup35 mutations. We observed its loss upon growth on guanidine hydrochloride and subsequent spontaneous reappearance with high frequency. The reversible curability of [ISP(+)] resembles the behavior of yeast prions. However, in contrast to known prions, [ISP(+)] does not depend on the chaperone protein Hsp104. Though manifestation of both [ISP(+)] and [PSI(+)] is related to the SUP35 gene, the maintenance of [ISP(+)] does not depend on the prionogenic N-terminal domain of Sup35p and Sup35p is not aggregated in [ISP(+)] cells, thus ruling out the possibility that [ISP(+)] is a specific form of [PSI(+)]. We hypothesize that [ISP(+)] is a novel prion involved in the control of translation accuracy in yeast.
Volkov
Kirill V
KV
Department of Genetics, St. Petersburg State University, St. Petersburg 199034, Russia.
Aksenova
Anna Yu
AY
Soom
Malle J
MJ
Osipov
Kirill V
KV
Svitin
Anton V
AV
Kurischko
Cornelia
C
Shkundina
Irina S
IS
Ter-Avanesyan
Michael D
MD
Inge-Vechtomov
Sergey G
SG
Mironova
Ludmila N
LN
D000483:Alleles; D015825:Chromosomes, Fungal; D003470:Culture Media / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005799:Genes, Dominant; D019791:Guanidine / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000502:physiology; D009687:Nuclear Proteins; D010454:Peptide Termination Factors; D011328:Prions / Q000235:genetics; D014176:Protein Biosynthesis / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D013489:Suppression, Genetic
D003470:Culture Media; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D010454:Peptide Termination Factors; D011328:Prions; C093988:Psi protein, S pombe; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C070972:HsP104 protein, S cerevisiae; D019791:Guanidine