Lacrimal gland fluid secretion and lymphocytic infiltration in the NZB/W mouse model of Sjögren's syndrome.
OBJECTIVE
The fluid secretory impairment of lacrimal and salivary glands in Sjögren's syndrome (SS) is thought to be related to the extent of lymphocytic infiltration (LI) and subsequent loss of glandular tissue. In this study, we examine the correlation between the extent of tear flow reduction and the extent of LI of lacrimal glands in the NZB/W mouse, a model of SS.


METHODS
We stimulated tear production by topical application of carbachol onto the gland while fluid was collected from the lacrimal duct. The lacrimal glands were removed after fluid collection for histology.


RESULTS
Fluid secretion in response to carbachol was less in the majority of young NZB/W females compared to C57 control animals and none of the glands showed LI. Fluid secretion was also impaired in the majority of old NZB/W females, and the extent of LI was highly variable. Some of the old SW females also showed blunted fluid secretory responses and some degree of focal LI. Young SW females showed no LI and most animals exhibited normal flow responses. Analysis of paired flow and LI measurements showed no correlation between LI and flow impairment in any of the groups or in the pooled data. Carbachol-stimulated protein secretion from lacrimal gland slices in vitro were similar in young and old SW and NZB/W mice.


CONCLUSIONS
These results suggest that LI alone is not sufficient to explain the secretory dysfunction in the NZB/W mouse model of Sjögren's syndrome.
Paranyuk
Y
Y
Department of Physiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5230, USA.
Claros
N
N
Birzgalis
A
A
Moore
L C
LC
Brink
P R
PR
Walcott
B
B
D000287:Administration, Topical; D000818:Animals; D002217:Carbachol / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D007765:Lacrimal Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D012859:Sjogren's Syndrome / Q000378:metabolism* / Q000473:pathology; D013666:Tears / Q000378:metabolism*
D002217:Carbachol