title: Nickel affects activity more than expression of hydrogenase protein in Frankia.
abstract: The effects of nickel on hydrogen uptake and the post-translational processing of the large subunit of the hydrogenase protein in three Frankia strains (one isolated from an Alnus-Frankia symbiosis and two from Casuarina-Frankia associations) were investigated. All three strains responded to the addition of nickel with an increase in hydrogen uptake. Additional nickel did not affect nitrogenase activity, however evolved hydrogen was detected in Frankia KB5 in the absence of additional nickel, indicating that hydrogenase was not active. No increase in the processing rate of the hydrogenase large subunit was found with increasing nickel concentrations for any of the strains, indicating that the strategy for regulating hydrogenase in Frankia is different from that in other microorganisms.
authors.affiliation: UPSC, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Mattsson
authors.forename: Anita
authors.initials: A
authors.lastname: Sellstedt
mesh_terms: D000192:Actinomycetales / Q000187:drug effects / Q000201:enzymology*; D015153:Blotting, Western; D005656:Fungal Proteins / Q000096:biosynthesis / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006859:Hydrogen / Q000378:metabolism / Q000493:pharmacokinetics; D006864:Hydrogenase / Q000096:biosynthesis* / Q000378:metabolism; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D009591:Nitrogenase / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects
chemical_list: D005656:Fungal Proteins; D009532:Nickel; D006859:Hydrogen; D006864:Hydrogenase; D009591:Nitrogenase