title: Determination of a non-methylated deoxycytidine residue in the recognition site of DNA-methyltransferases.
abstract: A method for determination of a non-methylated deoxycytidine (dC) residue in the recognition site of 5-cytosine DNA-methyltransferases is suggested. The method is based on treatment of methylated DNA by sodium bisulfite and successive reaction of the thus modified DNA with a repair enzyme, uracil-DNA glycosylase. This method was successfully applied to identify NlaX methyltransferase specificity.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899 Russia. kubareva@genebee.msu.su
authors.forename: E A
authors.initials: EA
authors.lastname: Kubareva
authors.forename: J
authors.initials: J
authors.lastname: Walter
authors.forename: O V
authors.initials: OV
authors.lastname: Vorob'eva
authors.forename: M V
authors.initials: MV
authors.lastname: Razumikhin
authors.forename: A S
authors.initials: AS
authors.lastname: Karyagina
authors.forename: P C
authors.initials: PC
authors.lastname: Lau
authors.forename: T
authors.initials: T
authors.lastname: Trautner
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D045647:DNA Glycosylases; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000378:metabolism*; D003841:Deoxycytidine / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009343:Neisseria / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D013447:Sulfites / Q000737:chemistry; D051981:Uracil-DNA Glycosidase
chemical_list: D011994:Recombinant Proteins; D013447:Sulfites; D003841:Deoxycytidine; D004247:DNA; D015254:DNA Modification Methylases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; C009279:sodium bisulfite