The expression and characterization of cyclin-dependent kinase 6 during the activation of murine G0 T-cells.
Recent evidence strongly suggest that the D type cyclins with cdk4 and cdk6 form holoenzymes that regulate cell cycle events earlier in G1 than cyclin E/cdk2 complexes which functions near the G1/S transition. In human T lymphocytes cdk6 has been shown to be the initial retinoblastoma protein kinase detectable at mid G1. Following activation of splenic derived murine G0T-cells, cdk6, cyclin D2 and D3 specific mRNAs were detected early in G1 and reached maximal levels prior to or near G1/S. The phosphorylation of retinoblastoma protein from T-cells was detected very early in G1 and was associated mainly with cdk6/cyclin D2 complexes which accounted for a minor portion of the total cellular cdk6 contained in the cytoplasmic fraction of T-cells and mostly in the catalytically inactive form.
Kwon
T K
TK
Department of Immunology, School of Medicine, Keimyung University, Taegu, Korea. kwontk@dsmc.or.kr
Park
J W
JW
Buchholz
M A
MA
Nordin
A A
AA
D000818:Animals; D000961:Antilymphocyte Serum; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases; D016213:Cyclins / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D000961:Antilymphocyte Serum; D016213:Cyclins; D011948:Receptors, Antigen, T-Cell; D017346:Protein Serine-Threonine Kinases; C495911:CDK6 protein, human; C495912:Cdk6 protein, mouse; D051361:Cyclin-Dependent Kinase 6; D018844:Cyclin-Dependent Kinases