[Frequent loss of fragile histidine triad gene transcripts in lung cancer].
OBJECTIVE
To investigate the significance of the fragile histidine triad (FHIT) gene in evaluation of early diagnosis and metastasis in lung cancer.


METHODS
47 lung cancer samples and 16 matched metastatic hilar lymph nodes of lung cancer patients were examined for abnormalities of the gene by using RT-PCR and RT-PCR-SSCP.


RESULTS
32 of 47 (68%) lung cancer samples and 15 of 16 metastatic hilar lymph nodes showed loss of FHIT gene transcripts (exon5-9 or exon1-4). RT-PCR-SSCP reveal no variants.


CONCLUSIONS
These data indicate that loss of FHIT gene transcript is frequent in lung cancer and may be an early event in lung tumorigenesis and FRA3B may be involved in the development and progression of the lung cancer.
Wang
X
X
Department of Medical Oncology, National Laboratory of Molecular Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021.
Sun
Y
Y
Li
W
W
Wang
X
X
Ding
F
F
Wu
M
M
D017766:Acid Anhydride Hydrolases; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000099170:Bis(5'-adenosyl)triphosphatase