title: Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis.
abstract: A monohalomethane-producing enzyme, S-adenosyl-L-methionine-dependent halide ion methyltransferase (EC 2.1.1.-) was purified from the marine microalga Pavlova pinguis by two anion exchange, hydroxyapatite and gel filtration chromatographies. The methyltransferase was a monomeric molecule having a molecular weight of 29,000. The enzyme had an isoelectric point at 5.3, and was optimally active at pH 8.0. The Km for iodide and SAM were 12 mM and 12 microM, respectively, which were measured using a partially purified enzyme. Various metal ions had no significant effect on methyl iodide production, suggesting that the enzyme does not require metal ions. The enzyme reaction strictly depended on SAM as a methyl donor, and the enzyme catalyzed methylation of the I-, Br-, and Cl- to corresponding monohalomethanes and of bisulfide to methyl mercaptan.
authors.affiliation: Biotechnology Research Center, Toyama Prefectural University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ohsawa
authors.forename: M
authors.initials: M
authors.lastname: Tsujita
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
authors.forename: N
authors.initials: N
authors.lastname: Itoh
mesh_terms: D002845:Chromatography; D056890:Eukaryota / Q000201:enzymology*; D006219:Halogens / Q000378:metabolism; D006846:Hydrocarbons, Halogenated / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D008780:Methyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D010839:Phytoplankton / Q000201:enzymology*; D012436:S-Adenosylmethionine / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D006219:Halogens; D006846:Hydrocarbons, Halogenated; D012436:S-Adenosylmethionine; D008780:Methyltransferases; C459582:S-adenosyl-L-methionine halide ion methyltransferase