title: Cytochrome P4501A responses in two tropical fish species, riverine catfish (Rita rita) and marine mudfish (Apocryptes bato).
abstract: The present study focuses on the establishment of methods for biomarker studies in freshwater and marine fish species as a basis for monitoring the extent of contamination of fisheries resources in tropical waters. Riverine catfish (Rita rita) and marine mudfish (Apocryptes bato) were given a single intraperitoneal injection with two selected inducing compounds; beta-naphthoflavone (BNF, 50 mg/kg) and a polychlorinated biphenyl mixture (Clophen A50, 20 mg/kg), and the heavy metal compound cadmium chloride (CdCl2, 1 mg/kg). Effects on cytochrome P4501A (CYP1A) were determined in post-mitochondrial supernatants (PMS) of liver at days 3 and 10 after treatment. EROD (7-ethoxyresorufin O-deethylase) activity and CYP1A protein level by indirect non-competitive enzyme-linked immunosorbent assay (ELISA) and Western blotting using a monoclonal antibody against fish CYP1A, were measured. BNF and Clophen A50 resulted in induction upto 9.5- and 5-fold, respectively, of CYP1A protein compared to control, while CdCl2 showed significant inhibition in these species. The present study examined the phase-I cytochrome P450 monooxygenase activity and response in these two tropical fish species for the first time.
authors.affiliation: Department of Fisheries and Marine Biology, University of Bergen, HIB, N-5020, Bergen, Norway. syed.al-arabi@ifm.uib.no
authors.forename: S A M
authors.initials: SA
authors.lastname: Al-Arabi
authors.forename: A
authors.initials: A
authors.lastname: Goksøyr
mesh_terms: D000818:Animals; D019256:Cadmium Chloride / Q000633:toxicity; D002397:Catfishes; D019363:Cytochrome P-450 CYP1A1 / Q000276:immunology / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D014874:Water Pollutants, Chemical / Q000032:analysis; D014876:Water Pollution / Q000032:analysis*; D019324:beta-Naphthoflavone / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1; D019256:Cadmium Chloride