[Experimental study on adhesion mechanism of airway inflammation in the pathogenesis of bronchial asthma].
OBJECTIVE
To investigate the adhesive mechanism of airway inflammation in the pathogenesis of asthma.


METHODS
The guniea pigs were divided into 2 groups, the asthmatic animal group and the normal animal group. The following tests were performed: (a) The values of sICAM-1, sP-selectin, IL-8 and ECP both in plasma (or serum) and BALF were measured by ELISA. (b) The expressions of ICAM-1 and IL-8 on the bronchial epithelial cells and vascular endothelial cells were measured by immuno-histochemistry staining. (c) The lung functions (VT, Cdyn, R(L)) were tested.


RESULTS
(a) The values of sICAM-1, sP-selectin and ECP were elevated both in the plasma and BALF level of asthmatic group (P < 0.01). Additionally, IL-8 of BALF level of asthmatic group was elevated (P < 0.01), while IL-8 of serum level was lower than that of normal group (P < 0.01). (b) The expressions of ICAM-1, IL-8 on the bronchial epithelial and vascular endothelial cells in asthmatic animals were up-regulated (P < 0.01). (c) The lung functions of the asthmatic animals were impaired (V(T), C(dyn) P < 0.01; R(L) P < 0.05).


CONCLUSIONS
ALL the results above suggests that ICAM-1, IL-8, P-selectin and ECP are involved in the inflammative adhesive mechanism of asthma.
Li
Y
Y
Department of Respiratory Medicine, Shanghai NO. 455 Hospital, Shanghai 200052.
Ye
Y
Y
D000818:Animals; D001249:Asthma / Q000473:pathology* / Q000503:physiopathology; D001798:Blood Proteins / Q000032:analysis / Q000502:physiology*; D002448:Cell Adhesion; D047091:Eosinophil Granule Proteins; D006168:Guinea Pigs; D007249:Inflammation / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology*; D016209:Interleukin-8 / Q000032:analysis / Q000502:physiology*; D008168:Lung / Q000503:physiopathology; D019007:P-Selectin / Q000032:analysis / Q000502:physiology*; D012260:Ribonucleases
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D016209:Interleukin-8; D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1; D012260:Ribonucleases