Participation of the mitochondrial permeability transition pore in nitric oxide-induced plant cell death.
In the present study, we investigated the involvement of the mitochondrial permeability transition pore (PTP) in nitric oxide (NO)-induced plant cell death. NO donors such as sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine inhibited growth and caused death in suspension-cultured cells of Citrus sinensis. Cells treated with SNP showed chromatin condensation and fragmentation, characteristic of apoptosis. SNP caused loss of the mitochondrial membrane electrical potential, which was prevented by cyclosporin A (CsA), a specific inhibitor of PTP formation. CsA also prevented the nuclear apoptosis and subsequent Citrus cell death induced by NO. These findings indicate that mitochondrial PTP formation is involved in the signaling pathway by which NO induces apoptosis in cultured Citrus cells.
Saviani
Elzira E
EE
Departmento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, SP, Brazil.
Orsi
Cintia H
CH
Oliveira
Jusceley F P
JF
Pinto-Maglio
Cecília A F
CA
Salgado
Ione
I
D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002957:Citrus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology; D053938:DNA Fragmentation; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D009569:Nitric Oxide / Q000633:toxicity*; D020030:Nitric Oxide Donors / Q000633:toxicity; D009599:Nitroprusside / Q000633:toxicity; D026423:S-Nitroso-N-Acetylpenicillamine / Q000633:toxicity; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D007473:Ion Channels; D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D020030:Nitric Oxide Donors; D009599:Nitroprusside; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D016572:Cyclosporine