title: Oligoclonal T cell expansions in pulmonary lymphoproliferative disorders: demonstration of the frequent occurrence of oligoclonal T cells in human immunodeficiency virus-related lymphoid interstitial pneumonia.
abstract: We used a denaturing gradient gel electrophoresis (DGGE) procedure with 40-nucleotide guanine- and cytosine-rich sequences in the polymerase chain reaction (PCR) and sequencing analysis to analyze the T cell antigen receptor (TCR)-Vgamma gene repertoire of infiltrating T lymphocytes in pulmonary lymphoproliferative disorders. Six of 15 low-grade mucosa-associated lymphoid tissue (MALT) lymphomas and 8 of 15 cases of lymphocytic interstitial pneumonia (LIP) showed some oligoclonal bands for TCR-Vgamma genes on DGGE. Sequencing analysis demonstrated plural oligoclonal TCR-Vgamma clones among the oligoclonal PCR products on DGGE, leading to the conclusion that conventional antigen-specific oligoclonal expansions may play some role in the pathogenesis of pulmonary lymphoproliferative disorders. The frequency of oligoclonal infiltrating T cell expansions in human immunodeficiency virus (HIV)-related LIP (100%) was significantly higher than in low-grade pulmonary MALT lymphomas (40%) or in HIV-negative LIP (30%). Because recent evidence demonstrates that the V3 loop in the proviral amino acid sequences of mononuclear cells from bronchoalveolar lavage is more homogeneous than those from peripheral blood, this homogeneity might result in oligoclonal expansions of infiltrating T lymphocytes as a consequence of ongoing reactions against lung-specific viral strains.
authors.affiliation: Department of Respirology, School of Medicine, Chiba University, Chiba, Japan. SNC16385@nifty.com
authors.forename: Katsushi
authors.initials: K
authors.lastname: Kurosu
authors.forename: Norio
authors.initials: N
authors.lastname: Yumoto
authors.forename: William N
authors.initials: WN
authors.lastname: Rom
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Takiguchi
authors.forename: Jagirdai
authors.initials: J
authors.lastname: Jaishree
authors.forename: Koh
authors.initials: K
authors.lastname: Nakata
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tatsumi
authors.forename: Aatsuo
authors.initials: A
authors.lastname: Mikata
authors.forename: Takatyuki
authors.initials: T
authors.lastname: Kuriyama
authors.forename: Michael D
authors.initials: MD
authors.lastname: Weiden
mesh_terms: D000328:Adult; D000368:Aged; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D017563:Lung Diseases, Interstitial / Q000150:complications* / Q000235:genetics* / Q000503:physiopathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000502:physiology; D016483:Lymphoma, AIDS-Related / Q000150:complications* / Q000235:genetics* / Q000503:physiopathology; D008232:Lymphoproliferative Disorders / Q000150:complications* / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D043025:Oligoclonal Bands; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000502:physiology; D017421:Sequence Analysis; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D007136:Immunoglobulins; D043025:Oligoclonal Bands; D011948:Receptors, Antigen, T-Cell