title: Effect of hexasulfobutylated C(60) on the isolated aortic ring of guinea pig.
abstract: The effects of hexasulfobutylated C(60) (FC(4)S) and monomalonic acid C(60) (MMA C(60)), the fullerene C(60) derivatives, on isolated endothelium-containing or endothelium-denuded aorta of guinea pig were studied pharmacologically in vitro. In the endothelium-containing preparation of the aortic rings, phenylephrine (PHE) elicited contracture and acetylcholine (ACh) elicited a relaxing effect on the PHE-precontracted preparation. In the PHE-precontracted preparation, MMA C(60) (10 micromol/l) did not elicit a relaxing effect on the PHE-precontracted preparation. However, MMA C(60) (10 micromol/l) significantly reduced the maximum response of the relaxation elicited by ACh. FC(4)S itself significantly relaxed the PHE-precontracted aortic rings. ACh-induced relaxation in PHE-precontracted endothelium-containing strips of the aortic rings was significantly potentiated if pretreated with FC(4)S (10 micromol/l). In the denuded aortic rings, FC(4)S did not elicit the relaxing effect in the PHE-precontracted preparation. The relaxing effect of FC(4)S on the PHE-precontracted preparation was not altered when superoxide dismutase (250 units/ml) was pretreated. However, the relaxing effect was reduced when N(G)-nitro-L-arginine methyl ester (L-NAME, 1 mmol/l) or methylene blue (1 micromol/l) was pretreated. These results demonstrated that the vasorelaxation effect of FC(4)S on the PHE-precontracted aortic ring is partly dependent on the release of nitric oxide (NO) or an NO-derived substance from the vascular endothelium.
authors.affiliation: Department of Pharmacology, College of Medicine, National Taiwan University Hospital, No. 1 Sec. 1, Jen-Ai Road, Taipei, Taiwan.
authors.forename: Shiang Suo
authors.initials: SS
authors.lastname: Huang
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Mashino
authors.forename: Masataka
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Long Y
authors.initials: LY
authors.lastname: Chiang
authors.forename: Lan Hui
authors.initials: LH
authors.lastname: Chih
authors.forename: Hung Ming
authors.initials: HM
authors.lastname: Hsieh
authors.forename: Chen Ming
authors.initials: CM
authors.lastname: Teng
authors.forename: Kensuke
authors.initials: K
authors.lastname: Okuda
authors.forename: Takasi
authors.initials: T
authors.lastname: Hirota
authors.forename: Ming Cheng
authors.initials: MC
authors.lastname: Tsai
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000502:physiology; D002244:Carbon / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D037741:Fullerenes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008314:Malonates / Q000494:pharmacology*; D008751:Methylene Blue / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D010656:Phenylephrine / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D037741:Fullerenes; D008314:Malonates; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C108688:monomalonic acid C(60); D010656:Phenylephrine; D009569:Nitric Oxide; D002244:Carbon; D013482:Superoxide Dismutase; D000109:Acetylcholine; C069837:fullerene C60; D008751:Methylene Blue; D019331:NG-Nitroarginine Methyl Ester