title: Molecular and physical characterization of Burkholderia mallei O antigens.
abstract: Burkholderia mallei lipopolysaccharide (LPS) has been previously shown to cross-react with polyclonal antibodies raised against B. pseudomallei LPS; however, we observed that B. mallei LPS does not react with a monoclonal antibody (Pp-PS-W) specific for B. pseudomallei O polysaccharide (O-PS). In this study, we identified the O-PS biosynthetic gene cluster from B. mallei ATCC 23344 and subsequently characterized the molecular structure of the O-PS produced by this organism.
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.
authors.forename: Mary N
authors.initials: MN
authors.lastname: Burtnick
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Brett
authors.forename: Donald E
authors.initials: DE
authors.lastname: Woods
mesh_terms: D019117:Burkholderia / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology; D003429:Cross Reactions; D005798:Genes, Bacterial; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D015610:Serum Bactericidal Test
chemical_list: D019081:O Antigens