title: Application of the biological conjugate between antibody and colloid Au nanoparticles as analyte to inductively coupled plasma mass spectrometry.
abstract: This paper describes the study of atomization of nanoparticles by inductively coupled plasma mass spectrometry (ICPMS) and developes a novel nonisotopic immunoassay by coupling sandwich-type immunoreaction to ICPMS. The goat-anti-rabbit immunoglobulin G (IgG) labeled with colloidal gold nanoparticles served as an analyte in ICPMS for the indirect measurement of rabbit-anti-human IgG. Matrix effect studies showed the gold signal was not sensitive to the organic matrix. A relatively good correlation (r2 = 0.9528) between the proposed method and enzyme-linked immunosorbent assay has been obtained. The method may have significant potential as an important ICPMS-based nonisotopic immnoassay method for the simultaneous determination of biologic analytes of interest by labeling different kinds of inorganic nanoparticles.
authors.affiliation: Department of Chemistry and Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Binbing
authors.initials: B
authors.lastname: Yu
authors.forename: Jinjun
authors.initials: J
authors.lastname: Shi
authors.forename: Xinrong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017979:Gold Colloid; D006801:Humans; D007118:Immunoassay; D018796:Immunoconjugates; D007136:Immunoglobulins / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D036103:Nanotechnology
chemical_list: D017979:Gold Colloid; D018796:Immunoconjugates; D007136:Immunoglobulins