Characterization of blaCMY-11, an AmpC-type plasmid-mediated beta-lactamase gene in a Korean clinical isolate of Escherichia coli.
We report the description of a new plasmid-encoded AmpC-type beta-lactamase gene (bla(CMY-11)) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pI determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the beta-lactamase gene (bla(CMY-11)) responsible for the cefoxitin resistance. CMY-11 and bla(CMY-11) are compared with other class C beta-lactamases and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A beta-lactamase with a pI value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing bla(CMY-11) was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that bla(CMY-11) belongs to the group of ampC-related bla genes. It is likely that bla(CMY-11) evolved from bla(CMY-1) via bla(CMY-10).
Lee
Sang Hee
SH
Department of Genetic Engineering, Youngdong University, Chungbuk 370-701, South Korea. sanghee@youngdong.ac.kr
Kim
Jae Young
JY
Lee
Gyu Sang
GS
Cheon
Seok Ho
SH
An
Young Jun
YJ
Jeong
Seok Hoon
SH
Lee
Kye Joon
KJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence / Q000235:genetics; D002440:Cefoxitin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000201:enzymology / Q000382:microbiology; D005260:Female; D006801:Humans; D007723:Korea / Q000453:epidemiology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D014552:Urinary Tract Infections / Q000201:enzymology / Q000382:microbiology; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D002440:Cefoxitin; C048795:AmpC beta-lactamases; D001618:beta-Lactamases