Unraveling the essential role in conjugation of the Tra protein of Streptomyces lividans plasmid pIJ101.
Plasmid pIJ101 from Streptomyces lividans encodes a single gene, tra, that is essential for both plasmid transfer and mobilization of chromosomes during mating. The tra gene product (Tra) is a membrane protein, a portion of which shows similarity to transfer proteins of other streptomycete plasmids as well as additional bacterial chromosome partitioning proteins. This paper reviews past and present work that has focused on elucidating the precise role of the Tra protein of pIJ101 in conjugation in Streptomyces.
Pettis
G S
GS
Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA. gpettis@unix1.sncc.lsu.edu
Cohen
S N
SN
D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D015964:Gene Expression Regulation, Bacterial; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; C085510:transfer region protein, Staphylococcus