[Effect of tiangui recipe on expressions of hypothalamic leptin receptor and messenger ribonucleic acid of neuropeptide Y in androgen-sterilized rats].
OBJECTIVE
To explore the effect of Tiangui Recipe (TGR) on obesity and anovulation in androgen-sterilized rats (ASR) from the gene transcription level.


METHODS
In situs hybridization and autoradiography were adopted, using alpha-32 P-radiolabelled leptin receptor (OB-R) and neuropeptide Y (NPY) oligonucleotides probes and integrating with image pattern analysis, to observe the effect of TGR on changes of OB-R, NPY mRNA expression in the hypothalamic arcuate nucleus (ARC).


RESULTS
Compared to the normal rats, the levels of hypothalamic OB-R mRNA expression decreased, NPY mRNA expression elevated significantly in ASR (P < 0.01). ASR characterized by obesity and anovulation. There was no significant difference in hypothalamic OB-R, NPY mRNA expressions between normal rats and ASR with TGR administration.


CONCLUSIONS
The increased hypothalamic NPY and decreased OB-R mRNA expression may be important contributing factors to the development of obesity and anovulation in ASR. TGR may play a role in reducing weight and inducing ovulation by regulating NPY/OB-R mRNA expression.
Sun
F
F
Obstetric and Gynecologic Hospital, Shanghai Medical University, Shanghai (200011).
Yu
J
J
Zhang
L
L
D000818:Animals; D000831:Animals, Newborn; D001111:Arcuate Nucleus of Hypothalamus / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism*; D007247:Infertility, Female / Q000139:chemically induced / Q000378:metabolism*; D009478:Neuropeptide Y / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D013739:Testosterone
D002352:Carrier Proteins; D004365:Drugs, Chinese Herbal; D009478:Neuropeptide Y; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D013739:Testosterone