title: [Isolation of lymphocytic choriomeningitis virus from human individuals].
abstract: The activity of lymphocytic choriomeningitis virus (LCMv) in Argentina has been previously reported on the basis of serological evidence in rodents and humans and the isolation of only one strain of LCMv from a Mus domesticus captured in the province of Córdoba. The aim of this paper was to register patients with serological diagnosis of LCM, to isolate and to identify human strains of LCMv in Argentina. During the last 19 years, 15 cases were diagnosed as LCM by immunoflourescent indirect assay (IFI) and enzyme-linked immunosorbent assay (ELISA) but when neutralizing assay (NT) was incorporated, eight cases were classified as confirmed, three as probable and four as negative. The geographic distribution of the cases included three provinces: Córdoba, Buenos Aires and Santa Fe. Viral isolation was attempted in five patients classified as confirmed and only two resulted positive (P5226 and P8573). They were identified as LCMv by IFI and NT. The coexistence of LCMv with other arenaviruses, such as Junin and Oliveros viruses, in the same area, raises the probability of interactions between them, which could modify the virulence and/or pathogenicity for humans associated to genomic changes. Future studies of antigenic, genomic and virulence variability of different Argentine strains of LCMv, as well as the systematic search for human infection, will contribute to define the importance of this viral agent in our country and to implement control measures.
authors.affiliation: Instituto Nacional de Entermedades Virales Humanas Dr. Julio I. Maiztegul, Monteagudo 2510, 2700 Pergamino, Buenos Aires, Argentina. carmensaavedra_ar@yahoo.com.ar
authors.forename: M C
authors.initials: MC
authors.lastname: Saavedra
authors.forename: A M
authors.initials: AM
authors.lastname: Ambrosio
authors.forename: L
authors.initials: L
authors.lastname: Riera
authors.forename: S
authors.initials: S
authors.lastname: Levis
authors.forename: J
authors.initials: J
authors.lastname: Sottosanti
authors.forename: M
authors.initials: M
authors.lastname: Sabattini
mesh_terms: D000818:Animals; D001118:Argentina; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D008216:Lymphocytic Choriomeningitis / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D008217:Lymphocytic choriomeningitis virus / Q000302:isolation & purification*; D009500:Neutralization Tests; D011817:Rabbits; D051381:Rats