title: Physical exercise induces activation of NF-kappaB in human peripheral blood lymphocytes.
abstract: Current understanding of nuclear factor-kappaB (NF-kappaB) activation is derived mostly from in vitro studies, and in vivo human data are limited. This study provides first evidence showing that physical exercise (80% maximal O2 consumption, 1 h) may trigger NF-kappaB activation, as determined by electrophoretic mobility shift assay, in peripheral blood lymphocytes of physically fit young men. Supershift assay showed that the NF-kappaB protein complex contained the transcriptionally active p65 protein. Plasma levels of NF-kappaB-directed gene products such as tumor necrosis factor-alpha and interleukin-2 receptor confirmed that physical exercise caused NF-kappaB transactivation. Exercise-induced NF-kappaB activation in lymphocytes was associated with elevated levels of lipid peroxidation by-products in the plasma.
authors.affiliation: Department of Surgery, Davis Heart & Lung Research Institute, The Ohio State University Medical Center, Columbus, USA. Jelena.Vider@kliinik.um.ee
authors.forename: J
authors.initials: J
authors.lastname: Vider
authors.forename: D E
authors.initials: DE
authors.lastname: Laaksonen
authors.forename: A
authors.initials: A
authors.lastname: Kilk
authors.forename: M
authors.initials: M
authors.lastname: Atalay
authors.forename: J
authors.initials: J
authors.lastname: Lehtmaa
authors.forename: M
authors.initials: M
authors.lastname: Zilmer
authors.forename: C K
authors.initials: CK
authors.lastname: Sen
mesh_terms: D000328:Adult; D015444:Exercise; D006801:Humans; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000097:blood*; D015375:Receptors, Interleukin-2 / Q000097:blood; D013997:Time Factors; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016328:NF-kappa B; D015375:Receptors, Interleukin-2; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha