[Research on expression of human papillomavirus type 16 and telomerase in oral lesions].
OBJECTIVE
To define a correlation between human papillomavirus (HPV) type 16 and telomerase activity during the carcinogenesis of oral mucosa.


METHODS
HPV16 DNA and human telomerase reverse transcriptase (hTRT) mRNA were detected in 82 cases of paraffin embedded tissues including 7 cases of normal oral mucosa, 7 cases of hyperplasia lesions, 30 cases of oral dysplasia lesions and 38 cases of oral squamous cell carcinomas (OSCCs) by PCR and in situ hybridization (ISH) respectively.


RESULTS
HPV16DNA was positive in 14.3% (1/7) of normal oral mucosa, 42.9% (3/7) of hyperplasia lesions, 66.6% (20/30) of dysplasia lesions and 92.1% (35/38) of OSCCs. hTRTmRNA was detectable in 30.0% (9/30) of oral dysplasia lesions and 81.6% (31/35) of OSCCs while normal oral mucosa and hyperplasia lesions were negative. Expression of HPV16DNA and hTRTmRNA were co-ordinate in 67.0% (55/82) cases.


CONCLUSIONS
HPV16 infection may play an important role in carcinogenesis of oral mucosa by activation of telomerase.
Patiman
Department of Oral and Maxillofacial Surgery, School of Stomatology, Shanghai Second Medical University, Shanghai 200011, China.
Zhang
Z
Z
Cao
J
J
D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D004268:DNA-Binding Proteins; D006801:Humans; D006965:Hyperplasia; D009061:Mouth Mucosa / Q000201:enzymology / Q000473:pathology / Q000821:virology; D009062:Mouth Neoplasms / Q000201:enzymology / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000235:genetics*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D019098:Telomerase