PRiMA: the membrane anchor of acetylcholinesterase in the brain.
As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. We have previously shown that collagen Q (ColQ) anchors AChE at the neuromuscular junction. We have now cloned the gene PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain. We show that PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. Furthermore, we demonstrate that AChE is actually anchored in neural cell membranes through its interaction with PRiMA. Finally, we propose that only PRiMA anchors AChE in mammalian brain and muscle cell membranes.
Perrier
Anselme L
AL
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS UMR 8544, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France.
Massoulié
Jean
J
Krejci
Eric
E
D000110:Acetylcholinesterase / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002091:Butyrylcholinesterase / Q000737:chemistry; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D003094:Collagen / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009865:Oocytes; D011392:Proline; D017434:Protein Structure, Tertiary; D014407:Tumor Cells, Cultured; D014981:Xenopus
D018076:DNA, Complementary; D008565:Membrane Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; C486718:prima1 protein, mouse; D003094:Collagen; D011392:Proline; D000110:Acetylcholinesterase; C114027:COLQ protein, human; D002091:Butyrylcholinesterase