title: Comparative study to quantify demineralized enamel in deciduous and permanent teeth using laser- and light-induced fluorescence techniques.
abstract: Caries is a disease that affects both deciduous and permanent dentitions. Caries progresses more rapidly in deciduous enamel than in permanent enamel. Therefore, new caries diagnostic methods need to be tested on the deciduous teeth as well. Quantitative laser-induced fluorescence (QLF I) as well as the quantitative light-induced fluorescence (QLF II) seem promising for the quantification of mineral loss from dental caries but have only been tested on the permanent dentition. The objective of this study was to determine and compare the ability of QLF I and QLF II to quantify mineral loss from carious lesions in both deciduous and permanent teeth. Thirty sound deciduous and 30 sound permanent teeth were cleaned and divided into three groups each containing 10 deciduous and 10 permanent teeth. Windows on the buccal or labial enamel surfaces were demineralized for 48, 72, or 96 h. Images of demineralized enamel were captured using QLF I and QLF II. The images were analyzed to determine the mean change in fluorescence radiance (Delta F, %). The teeth were then sectioned for assessment of lesion depth (microm) and integrated mineral loss (IML, vol% x microm) using transverse microradiography (TMR), as the 'gold standard' for lesion analysis. The results indicated a good correlation for Delta F between QLF I and QLF II in both deciduous (r = 0.96) and permanent teeth (r = 0.98). There was a good correlation between Delta F and TMR (lesion depth and IML) in deciduous teeth (r = 0.76 and 0.84 with QLF I, r = 0.81 and 0.88 with QLF II). In permanent teeth, the correlation between Delta F and TMR (lesion depth and IML) was lower than in deciduous teeth (r = 0.07 and 0.53 with QLF I, r = 0.15 and 0.62 with QLF II). From these results it can be concluded that either QLF method is capable of quantifying mineral loss in early carious lesions in deciduous teeth. Moreover, under the conditions of this study, the use of either QLF method to quantify mineral loss in early carious lesions in deciduous teeth is slightly more accurate than in permanent teeth.
authors.affiliation: Department of Preventive and Community Dentistry, Oral Health Research Institute, Indiana University School of Dentistry, Indianapolis, IN 46202-2876, USA. mando@iupui.edu
authors.forename: M
authors.initials: M
authors.lastname: Ando
authors.forename: M H
authors.initials: MH
authors.lastname: van Der Veen
authors.forename: B R
authors.initials: BR
authors.lastname: Schemehorn
authors.forename: G K
authors.initials: GK
authors.lastname: Stookey
mesh_terms: D000704:Analysis of Variance; D001128:Argon; D003731:Dental Caries / Q000175:diagnosis / Q000473:pathology; D003743:Dental Enamel / Q000473:pathology*; D018450:Disease Progression; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007834:Lasers; D008027:Light; D008852:Microradiography; D008903:Minerals / Q000032:analysis; D013223:Statistics as Topic; D014070:Tooth / Q000473:pathology*; D017001:Tooth Demineralization / Q000175:diagnosis* / Q000473:pathology; D014094:Tooth, Deciduous / Q000473:pathology*
chemical_list: D008903:Minerals; D001128:Argon