[Effect of the SH-group of myelopeptide MP-3 on its macrophage-stimulating activity].
Peptide Leu-Val-Cys-Tyr-Pro-Gln, identical to the bone marrow peptide MP-3, and its Val3 and Ser3 analogs, lacking SH-group, were synthesized by conventional methods of peptide chemistry in solution and, along with the MP-3 S-S-dimerization product, were studied with respect to their effect on the macrophage phagocytic activity. It was shown that the activity was only enhanced by peptide MP-3, which demonstrated the essential role of the SH-group in this function. The dimer analog of MP-3, unlike dimer analogs of other monocysteine-containing peptides, glutathione and HP5b, did not exhibit the inhibitory effect.
Fonina
L A
LA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. stas@ibch.ru
Baldin
M I
MI
Efremov
M A
MA
Gur'ianov
S A
SA
Belevskaia
R G
RG
D000595:Amino Acid Sequence; D001853:Bone Marrow / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D008262:Macrophage Activation / Q000187:drug effects*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
D009842:Oligopeptides; D013438:Sulfhydryl Compounds; C048617:myelopeptides