title: A unique type II restriction endonuclease FspAI, that recognizes the octanucleotide sequence 5'-RTGC/GCAY-3'.
abstract: A new type II restriction endonuclease designated FspAI has been partially purified from a Flexibacter species Tv-m21K. FspAI recognizes the octanucleotide sequence 5'-RTGC/GCAY-3' and cleaves it in the center generating blunt-ended DNA fragments.
authors.affiliation: MBI Fermentas, and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
authors.forename: A
authors.initials: A
authors.lastname: Kesminiene
authors.forename: Z
authors.initials: Z
authors.lastname: Maneliene
authors.forename: J
authors.initials: J
authors.lastname: Vitkute
authors.forename: M
authors.initials: M
authors.lastname: Petrusyte
authors.forename: A
authors.initials: A
authors.lastname: Janulaitis
mesh_terms: D001419:Bacteria / Q000201:enzymology; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009841:Oligonucleotides; D004247:DNA; C452019:endodeoxyribonuclease FspAI; D015252:Deoxyribonucleases, Type II Site-Specific