[Expression of recombinant BPI23-Fc gamma 1 fusion protein in CHO cells].
The fusion gene of BPI23 and human Fc gamma 1 was obtained by PCR method, and the expression plasmid was constructed to express recombinant BPI23-Fc gamma 1 fusion protein in CHO cells. After transfection with the plasmid and selection by methotrexate, the cell lines expressing the fusion protein were obtained. The recombinant protein was purified using cation-exchange chromatography and its bioactivity was proved with bactericidal assays.
Xu
J J
JJ
Institute of Microbiology and Epidemiology, Beijing 100071, China. xujunjie@sina.com
Xu
J
J
Tong
Y G
YG
Wang
H T
HT
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000235:genetics*; D016466:CHO Cells; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D017452:Receptors, IgG / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D008565:Membrane Proteins; D017452:Receptors, IgG; D011993:Recombinant Fusion Proteins; C021623:bactericidal permeability increasing protein