title: Study on the anticarcinogenic effect and acute toxicity of liver-targeting mitoxantrone nanoparticles.
abstract: AIM:To study the anticarcinogenic effect and acute toxicity of liver targeting mitoxantrone nanospheres.METHODS:The anticarcinogenic effect of mitoxan-trone polybutylcyanoacrylate nanoparticles (DHAQ-PBCA-NP) was investigated by using heterotopic and orthotopic transplantation models of human hepatocellular carcinoma (HCC) in nude mice and was compared with mitoxantrone (DHAQ) and doxorubicin (ADR).The acute toxicity of DHAQ-PBCA-NP lyophilized injection in mice was also studied.RESULTS:The tumor inhibition rates of ADR, DHAQ, DHAQ-PBCA-NP to orthotopically transplanted HCC were 60.07%, 67.49% and 99.44%, respectively, but regard to heterotopically transplanted HCC, these were 80.03%, 86.18% and 92.90%, which were concordant with the results acquired by mitosis counting and proliferating cell nuclear antigen (PCNA). After iv administration to mice with DHAQ-PBCA-NP, the LD(50) was 16.9mg/kg ± 3.9mg/kg, no obvious local irritation was observed and there was no significant damage to the structure of liver cells, and that of the heart, spleen and kidneys.CONCLUSION:The effect of DHAQ-PBCA-NP was significantly higher than that of DHAQ and ADR in the anti orthotopically transplanted HCC and the acute toxicity was relatively low.
authors.forename: Zhi-Rong
authors.initials: ZR
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: He
authors.forename: Gong-Tie
authors.initials: GT
authors.lastname: Liao
authors.forename: Shao-Huai
authors.initials: SH
authors.lastname: Bai