[The engulfment effects of macrophage on apoptotic eosinophils treated by anti-Fas monoclonal antibody in vitro].
OBJECTIVE
Expression of the Fas antigen and induction of apoptosis in eosinophils (EOS) by anti-Fas antibody in vitro were investigated.


METHODS
The purified EOS from healthy donors were cultured for 72 h in the presence of human IL-5 and with or without anti-Fas monoclonal antibody (MPhiAb) at various concentrations (1 approximately 1 000 ng/ml). The changes of eosinophil viability, percentage of apoptosis, and fragmented DNA electrophoresis were observed. The eosinophils treated by Fas MPhiAb for 24 h, 48 h, 72 h were cultured with monocyte-derived macrophages, the positive percentage of macrophages which engulfed EOS was calculated.


RESULTS
The anti-Fas MoAb suppressed IL-5-mediated EOS survival in dose- and time-dependant manner. High concentration of IL-5 (10(6)U/L) could not inhibit anti-Fas MoAb-mediated apoptosis. The percentage of apoptosis was (35 +/- 6)% after 24 h treated with Fas MoAb (P < 0.01), and increased to (96 +/- 3)% after 72 h (P < 0.01). The percentage of macrophages which engulfed eosinophils was significantly higher than control (P < 0.01).


CONCLUSIONS
The anti-Fas MoAb can efficiently induced eosinophils apoptosis, apoptotic EOS induced by anti-Fas MoAb could be engulfed by macrophages.
Lai
K
K
Institute of Respiratory Medicine of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Wang
C
C
Guo
X
X
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D013997:Time Factors; D019014:fas Receptor / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015848:Interleukin-5; D019014:fas Receptor; D004247:DNA