title: An impairment of barrier size and charge selectivity of glomerular basement membrane in streptozotocin-induced diabetes and prevention by pharmacological therapy.
abstract: We examined barrier size (pore size) and charge selectivity (anionic sites) of the glomerular basement membrane (GBM) in experimental diabetes. For estimation of the pore size we employed a new method, tissue negative staining. In diabetic rats, enlarged pores and decreased numbers of anionic sites of GBM were observed. Both insulin treatment and angiotensin-converting enzyme inhibitors prevented these changes. The renoprotective effect of the two drugs is discussed in the article.
authors.affiliation: Department of Medicine, Yamauchi Hospital, 4-6 Minamifujisawa, Fujisawa, Kanagawa 251-0055, Japan. yoshino@med.toho-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Isogai
authors.forename: K
authors.initials: K
authors.lastname: Mogami
authors.forename: H
authors.initials: H
authors.lastname: Ouchi
authors.forename: G
authors.initials: G
authors.lastname: Yoshino
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000838:Anions; D001485:Basement Membrane / Q000187:drug effects / Q000473:pathology*; D002216:Captopril / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology* / Q000503:physiopathology; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000838:Anions; D013311:Streptozocin; D002216:Captopril