Isolation of cicadin, a novel and potent antifungal peptide from dried juvenile cicadas.
A single-chained antifungal protein with a molecular weight of 6.5 kDa and displaying a novel N-terminal sequence was isolated from dried juvenile cicadas which are used in traditional Chinese medicine, by using ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and then gel filtration on a Superdex peptide column. The peptide, designated cicadin, exerted potent antifungal activity with IC(50) values at nonomolar concentrations against a variety of fungi including Botrytis cinerea, Mycosphaerella arachidicola, Fusarium oxysporum, Rhizoctonia solani and Coprinus comatus. Cicadin suppressed the activity of HIV-1 reverse transcriptase and stimulated the proliferation of murine splenocytes.
Wang
Hexiang
H
Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.
Ng
Tzi Bun
TB
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002455:Cell Division; D002474:Cell-Free System; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003636:DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D006430:Hemiptera; D020128:Inhibitory Concentration 50; D019476:Insect Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012685:Sepharose / Q000737:chemistry; D020539:Sequence Analysis, Protein; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D000935:Antifungal Agents; D019476:Insect Proteins; D010455:Peptides; D012685:Sepharose; D003636:DEAE-Cellulose; D054303:HIV Reverse Transcriptase