The dipole moment of membrane proteins: potassium channel protein and beta-subunit.
The mechanism of ion channel opening is one of the most fascinating problems in membrane biology. Based on phenomenological studies, early researchers suggested that the elementary process of ion channel opening may be the intramembrane charge movement or the orientation of dipolar proteins in the channel. In spite of the far reaching significance of these hypotheses, it has not been possible to formulate a comprehensive molecular theory for the mechanism of channel opening. This is because of the lack of the detailed knowledge on the structure of channel proteins. In recent years, however, the research on the structure of channel proteins made marked advances and, at present, we are beginning to have sufficient information on the structure of some of the channel proteins, e.g. potassium-channel protein and beta-subunits. With these new information, we are now ready to have another look at the old hypothesis, in particular, the dipole moment of channel proteins being the voltage sensor for the opening and closing of ion channels. In this paper, the dipole moments of potassium channel protein and beta-subunit, are calculated using X-ray diffraction data. A large dipole moment was found for beta-subunits while the dipole moment of K-channel protein was found to be considerably smaller than that of beta-subunits. These calculations were conducted as a preliminary study of the comprehensive research on the dipolar structure of channel proteins in excitable membranes, above all, sodium channel proteins.
Takashima
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Department of Bioengineering, University of Pennsylvania, 240 South 33rd Street, Philadelphia, PA 19104-6392, USA. takashim@eniac.seas.upenn.edu
D000818:Animals; D030562:Databases, Protein; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D015221:Potassium Channels / Q000737:chemistry*; D011487:Protein Conformation; D021122:Protein Subunits; D012399:Rotation; D015222:Sodium Channels / Q000737:chemistry; D055672:Static Electricity; D014961:X-Ray Diffraction
D008565:Membrane Proteins; D015221:Potassium Channels; D021122:Protein Subunits; D015222:Sodium Channels