A Saccharomyces cerevisiae gene required for heterologous fatty acid elongase activity encodes a microsomal beta-keto-reductase.
A number of Saccharomyces cerevisiae membrane-bound oxidoreductases were examined for potential roles in microsomal fatty acid elongation, by assaying heterologous elongating activities in individual deletion mutants. One yeast gene, YBR159w, was identified as being required for activity of both the Caenorhabditis elegans elongase PEA1 (F56H11.4) and the Arabidopsis thaliana elongase FAE1. Ybr159p shows some limited homology to human steroid dehydrogenases and is a member of the short-chain alcohol dehydrogenase superfamily. Disruption of YBR159w is not lethal, in contrast to previous reports, although the mutants are slow growing and display high temperature sensitivity. Both Ybr159p and an Arabidopsis homologue were shown to restore heterologous elongase activities when expressed in ybr159Delta mutants. Biochemical characterization of microsomal preparations from ybr159Delta cells revealed a primary perturbation in beta-ketoacyl reduction, confirming the assignment of YBR159w as encoding a component of the microsomal elongase.
Beaudoin
Frédéric
F
Institute of Arable Crops Research-Long Ashton Research Station, Long Ashton, Bristol BS41 9AF, United Kingdom.
Gable
Ken
K
Sayanova
Olga
O
Dunn
Teresa
T
Napier
Johnathan A
JA
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D000081031:Fatty Acid Elongases; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D017931:DNA Primers; D015094:3-Hydroxyacyl CoA Dehydrogenases; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases