[Analysis of the polymorphisms of the AACT, ERAB and NACP genes in 15 patients with Alzheimer's disease].
OBJECTIVE
To analyze the polymorphisms of the AACT, ERAB and NACP genes in Han Chinese Alzheimer's disease (AD) patients.


METHODS
Denaturant gradient gel electrophoresis (DGGE) was used to scan all DNA fragments of 3 genes mentioned above in AD group (15 cases) and elderly control group (241 cases).


RESULTS
A25G and G39A substitutions were found in the AACT gene. The gene frequency of A25G was 80% in the AD group and 47% in the control group (P < 0.05). The gene frequency of G39A genotype was 13% in the AD group and 0.4% in the control group (P < 0.01). The allele (39) frequencies were also significantly different between the two groups (P < 0.01). We did not find any points with polymorphism in the ERAB and NACP genes.


CONCLUSIONS
A25G and G39A substitutions in the AACT gene are probably one of the risk factors to AD in Han Chinese.
Cui
S
S
Shanghai Huadong Hospital/Shanghai Geriatrics Institute, Shanghai 200040, China.
Yu
Z
Z
Wu
H
H
D015094:3-Hydroxyacyl CoA Dehydrogenases; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D051843:Synucleins; D000514:alpha 1-Antichymotrypsin / Q000235:genetics*
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D051843:Synucleins; D000514:alpha 1-Antichymotrypsin; D004247:DNA; D015094:3-Hydroxyacyl CoA Dehydrogenases; C108634:HSD17B10 protein, human; C458480:Hsd17b10 protein, mouse