title: APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos.
abstract: Early embryonic cells in Caenorhabditis elegans embryos interact through a signaling pathway closely related to the Notch signaling pathway in Drosophila and vertebrates. Components of this pathway include a ligand, receptor, the presenilin proteins, and a novel protein, APH-2, that is related to the Nicastrin protein in humans. Here we identify the aph-1 gene as a new component of the Notch pathway in Caenorhabditis elegans. aph-1 is predicted to encode a novel, highly conserved multipass membrane protein. We show that aph-1 and the presenilin genes share a similar function in that they are both required for proper cell-surface localization of APH-2/Nicastrin.
authors.affiliation: Department of Biology, Amherst College, Amherst, MA 01002, USA. cegoutte@amherst.edu
authors.forename: Caroline
authors.initials: C
authors.lastname: Goutte
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunozaki
authors.forename: Valerie A
authors.initials: VA
authors.lastname: Hale
authors.forename: James R
authors.initials: JR
authors.lastname: Priess
mesh_terms: D000595:Amino Acid Sequence; D053829:Amyloid Precursor Protein Secretases; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics* / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D051880:Receptors, Notch; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: C405066:APH-1 protein, C elegans; D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D018398:Homeodomain Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D051880:Receptors, Notch; C410543:aph-2 protein, C elegans; C413604:nicastrin protein; D053829:Amyloid Precursor Protein Secretases