The effects of freezing and cryoprotectant exposure on adenylate cyclase activity in cell membranes of bovine thyroid gland and adrenal cortex.
We present data on the effect of freezing with or without cryoprotectant (CPA) dimethyl sulphoxide (Me(2)SO), as well as effects of exposure to CPA alone on the activity of adenylate cyclase in cellular membranes of thyroid glands and adrenal cortex. Our results show that freezing without cryoprotectant increases unstimulated adenylate cyclase activity and decreases sensitivity to specific regulators. CPA at a concentration of 5% for adenylate cyclase in adrenal cortex tissue and of 10% in thyroid gland allowed preservation of the best responses to specific receptor agonists.
Karpenko
L G
LG
Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 61015, 23, Pereyaslavskaya Str., Kharkov, Ukraine.
Gubina
N F
NF
Schirova
V A
VA
Kaprelyants
A S
AS
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000302:Adrenal Cortex / Q000187:drug effects / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D012969:Sodium Fluoride / Q000494:pharmacology; D013961:Thyroid Gland / Q000187:drug effects / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology
D003451:Cryoprotective Agents; D012969:Sodium Fluoride; D000324:Adrenocorticotropic Hormone; D013972:Thyrotropin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004121:Dimethyl Sulfoxide