[The effects of nifedipine on proliferation and DNA synthesis of gingival fibroblasts].
OBJECTIVE
To investigate the effects of nifedipine, a calcium channel blocker, on cellular proliferation in gingival fibroblasts in vitro.


METHODS
Gingival fibroblasts were cultured and incubated with various concentrations of nifedipine (1,200.0, 360.0, 108.0 and 32.4 micrograms/L) for up to 7 days. H-TdR incorporation assay in vitro taken was used to evaluate the amount of DNA synthesis.


RESULTS
Cell numbers were significantly different among these groups on sixth and seventh day, especially higher in 1,200.0 micrograms/L and 360.0 micrograms/L groups. The incorporation percentage in high concentration groups were significantly higher than those in others.


CONCLUSIONS
Nifedipine can influent proliferation of gingival fibroblasts in high concentration.
Sun
W
W
Department of Oral Medicine, School of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Jiang
C
C
Zheng
S
S
D000293:Adolescent; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002648:Child; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D009543:Nifedipine / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D004247:DNA; D009543:Nifedipine