The tylosin-biosynthetic genes of Streptomyces fradiae.
The tylosin-biosynthetic (tyl) gene cluster occupies about 1% of the genome of Streptomycesfradiae and includes at least 43 open reading frames. In addition to structural genes required for tylosin production, the tyl cluster contains three resistance determinants and several regulatory genes. Tylosin production is evidently controlled by pathway-specific and pleiotropic regulators with the likely involvement of y-butyrolactone signalling factors. Accumulation of the polyketide aglycone is controlled by glycosylated macrolides and optimal performance of the complex polyketide synthase enzyme requires the activity of an editing thioesterase.
Cundliffe
E
E
Department of Biochemistry, University of Leicester, UK. ec13@le.ac.uk
Bate
N
N
Butler
A
A
Fish
S
S
Gandecha
A
A
Merson-Davies
L
L
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005810:Multigene Family; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*; D015645:Tylosin / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015645:Tylosin