title: Inactivation of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on apples, oranges, and tomatoes by lactic acid with hydrogen peroxide.
abstract: The objective of this study was to develop a practical and effective method for inactivating or substantially reducing Escherichia coli O157:H7, Salmonella Enteritidis, and Listeria monocytogenes on apples, oranges, and tomatoes. Apples, oranges, and tomatoes were spot-inoculated with five-strain mixtures of E. coli O157:H7, Salmonella Enteritidis, and L. monocytogenes near the stem end and were submerged in sterile deionized water containing 1.5% lactic acid plus 1.5% hydrogen peroxide for 15 min at 40 degrees C. Inoculated samples treated with sterile deionized water at the same temperature and for the same duration served as controls. The bacterial pathogens on fruits subjected to the chemical treatment were reduced by >5.0 log10 CFU per fruit, whereas washing in deionized water decreased the pathogens by only 1.5 to 2.0 log10 CFU per fruit. Furthermore, substantial populations of the pathogens survived in the control wash water, whereas no E. coli O157:H7, Salmonella Enteritidis, or L. monocytogenes cells were detected in the chemical treatment solution. The sensory and qualitative characteristics of apples treated with the chemical wash solution were not adversely affected by the treatment. It was found that the treatment developed in this study could effectively be used to kill E. coli O157:H7, Salmonella Enteritidis, and L. monocytogenes on apples, oranges, and tomatoes at the processing or packaging level.
authors.affiliation: Center for Food Safety, University of Georgia, Griffin 30223-1797, USA.
authors.forename: Kumar S
authors.initials: KS
authors.lastname: Venkitanarayanan
authors.forename: Chia-min
authors.initials: CM
authors.lastname: Lin
authors.forename: Hannalore
authors.initials: H
authors.lastname: Bailey
authors.forename: Michael P
authors.initials: MP
authors.lastname: Doyle
mesh_terms: D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development; D005511:Food Handling / Q000379:methods*; D005638:Fruit / Q000382:microbiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D019344:Lactic Acid / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D012477:Salmonella enteritidis / Q000187:drug effects / Q000254:growth & development; D013696:Temperature; D014675:Vegetables / Q000382:microbiology*; D014867:Water
chemical_list: D004202:Disinfectants; D014867:Water; D019344:Lactic Acid; D006861:Hydrogen Peroxide