RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA).
Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.
Verbeure
B
B
Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Lescrinier
E
E
Wang
J
J
Herdewijn
P
P
D003510:Cyclohexanes / Q000737:chemistry* / Q000378:metabolism; D053138:Cyclohexenes; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016914:Ribonuclease H / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
D003510:Cyclohexanes; D053138:Cyclohexenes; D009841:Oligonucleotides; C052568:cyclohexene; D012313:RNA; D016914:Ribonuclease H