title: The presence of simian-virus 40 sequences in mesothelioma and mesothelial cells is associated with high levels of vascular endothelial growth factor.
abstract: The aim of this study was to evaluate whether the presence of simian virus-40 (SV40) is associated with increased release of vascular endothelial growth factor (VEGF) in human malignant mesothelioma (MM) cells. We studied nine cell lines derived from pleural effusion (PE) of patients with MM, and three different cultures of normal human mesothelial cells (NHMC) derived from pleural fluid of patients with congestive heart failure. NHMC were transfected with full length SV40 (NHMC-FL) or large T antigen (NHMC Tag) DNAs. High levels of VEGF were detected in conditioned media of each of two MM cells that tested positive for SV40 by PCR amplification and Southern blot hybridization and for Tag transcript by reverse transcription- polymerase chain reaction (RT-PCR) and immunoprecipitation. We also found that NHMC-FL released high amounts of VEGF. Conditioned media from SV40-positive MM cells and from FL-NHMC increased proliferation of human umbilical vein cells (HUVEC) and this effect was partially abrogated by adding specific blocking antibodies against VEGF. These results offer the first evidence that SV40 can cause VEGF release in SV40-positive MM cells and that entire viral genome is required for this effect.
authors.affiliation: Department of Medical Sciences, University of Piemonte Orientale A. Avogadro, Novara, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Cacciotti
authors.forename: Luigi
authors.initials: L
authors.lastname: Strizzi
authors.forename: Giovina
authors.initials: G
authors.lastname: Vianale
authors.forename: Laura
authors.initials: L
authors.lastname: Iaccheri
authors.forename: Roberta
authors.initials: R
authors.lastname: Libener
authors.forename: Camillo
authors.initials: C
authors.lastname: Porta
authors.forename: Mauro
authors.initials: M
authors.lastname: Tognon
authors.forename: Giovanni
authors.initials: G
authors.lastname: Gaudino
authors.forename: Luciano
authors.initials: L
authors.lastname: Mutti
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D017077:Culture Media, Conditioned; D016228:Endothelial Growth Factors / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000821:virology*; D004848:Epithelium / Q000378:metabolism / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D008222:Lymphokines / Q000378:metabolism*; D008654:Mesothelioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D010997:Pleural Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology; D013539:Simian virus 40 / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D000952:Antigens, Polyomavirus Transforming; D017077:Culture Media, Conditioned; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors