Molecular analysis of rifampin resistance in Bacillus anthracis and Bacillus cereus.
Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 x 10(-9) mutations/generation by a Luria-Delbrück fluctuation test.
Vogler
Amy J
AJ
Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.
Busch
Joseph D
JD
Percy-Fine
Stephanie
S
Tipton-Hunton
Christine
C
Smith
Kimothy L
KL
Keim
Paul
P
D000595:Amino Acid Sequence; D001408:Bacillus anthracis / Q000187:drug effects / Q000235:genetics*; D001409:Bacillus cereus / Q000187:drug effects / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
D010940:Plant Proteins; C101305:RpoB protein, Oryza sativa; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin