Anal cancer in Chinese: human papillomavirus infection and altered expression of p53.
AIM:To detect the presence of HPV DNA and study the alteration of p53 expression in anal cancers in Chinese.METHODS:HPV DNA was amplified by PCR.The amplified HPV DNA was classified by DBH. HPV antigen and p53 expression were respectively detected by immunohistochemistry.RESULTS:HPV DNA was amplified only in one case of squamous cell carcinoma of the 72 Chinese anal cancers and further classified as HPV type 16. Others were all HPV negative. HPV antigen and p53 expression were also detected in this case. Positive stainings with anti-p53 antibody were seen in 61.2% anal cancers.There were no statistically significant differences between anal squamous cell carcinomas and adenocarcinomas and between anal adenocarcinomas and rectal adenocarcinomas.p53 protein expression was observed in the basal cells of squamous epithelium of condyloma acuminatum and morphologically normal squamous epithelium in 2 cases invaded by anal adenocarcinoma.CONCLUSION:HPV infection was not associated with these cases of anal cancer.p53 alteration was a common event. Positive p53 immunostaining can not be regarded as a marker for differentiating benign from malignant lesions.
Lai
Mao-De
MD
Luo
Min-Jie
MJ
Yao
Jian-Er
JE
Chen
Pei-Hui
PH