Vascularized three-dimensional skeletal muscle tissue-engineering.
Medical science continues to battle against the loss or failure of organs or tissues. Since, skeletal muscle loss lead not only to the functional compromise of the affected site, but also a structural deformation; tissue engineering of skeletal muscle attempts to provide solutions to replace loss of tissue contour and function. In our study, myoblasts seeded onto polyglycolic acid (PGA) meshes were used to engineer skeletal muscle tissue in vivo. The cell-polymer constructs harvested after a period of 6-weeks were well vascularized three-dimensional structures with the ability to generate neo-muscle-like tissue. This is the first time that the ability of myoblasts to survive in vivo in the absence of mature skeletal muscle tissue was demonstrated. The successful ability to transplant myoblasts using biodegradable polymer strands without using the traditional transplantation buffer mediums as carriers was also employed for the first time.
Saxena
A K
AK
Department of Surgery, Children's Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts, MA 02511, USA. aksaxena@mail.com
Willital
G H
GH
Vacanti
J P
JP
D000818:Animals; D001706:Biopsy; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D008422:Materials Testing; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000166:cytology*; D011100:Polyglycolic Acid / Q000592:standards*; D051381:Rats; D011916:Rats, Inbred F344; D013526:Surgical Mesh / Q000592:standards*; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods* / Q000592:standards*
D011100:Polyglycolic Acid