title: Dendritic cells transport conidia and hyphae of Aspergillus fumigatus from the airways to the draining lymph nodes and initiate disparate Th responses to the fungus.
abstract: Aspergilli are respiratory pathogens and pulmonary infections are usually acquired through the inhalation of conidia, able to reach small airways and the alveolar space where the impaired host defense mechanisms allow hyphal germination and subsequent tissue invasion. The invasive pulmonary aspergillosis is the most common manifestation of Aspergillus fumigatus infection in immunocompromised patients and is characterized by hyphal invasion and destruction of pulmonary tissue. A Th1/Th2 dysregulation and a switch to a Th2 immune response may contribute to the development and unfavorable outcome of invasive pulmonary aspergillosis. Dendritic cells (DC) have a primary role in surveillance for pathogens at the mucosal surfaces and are recognized as the initiators of immune responses to them. In the present study, we assessed the functional activity of pulmonary DC in response to A. fumigatus conidia and hyphae, both in vitro and in vivo. We analyzed mechanisms and receptors for phagocytosis by DC as well as DC migration, maturation, and Th priming in vivo upon exposure to either form of the fungus. We found a remarkable functional plasticity of DC in response to the different forms of the fungus, as pulmonary DC were able to: 1) internalize conidia and hyphae of A. fumigatus through distinct phagocytic mechanisms and recognition receptors; 2) discriminate between the different forms in terms of cytokine production; 3) undergo functional maturation upon migration to the draining lymph nodes and spleens; and 4) instruct local and peripheral Th cell reactivity to the fungus.
authors.affiliation: Microbiology and Anatomy Sections, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Via del Giochetto, 06122 Perugia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bozza
authors.forename: Roberta
authors.initials: R
authors.lastname: Gaziano
authors.forename: Antonio
authors.initials: A
authors.lastname: Spreca
authors.forename: Angela
authors.initials: A
authors.lastname: Bacci
authors.forename: Claudia
authors.initials: C
authors.lastname: Montagnoli
authors.forename: Paolo
authors.initials: P
authors.lastname: di Francesco
authors.forename: Luigina
authors.initials: L
authors.lastname: Romani
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000276:immunology / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000276:immunology; D011975:Receptors, Mitogen / Q000502:physiology; D013172:Spores, Fungal / Q000276:immunology / Q000648:ultrastructure; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000382:microbiology; D013909:Thorax
chemical_list: D016207:Cytokines; D011975:Receptors, Mitogen