title: The evaluation of putative virulence factors of Helicobacter pylori for gastroduodenal disease by use of a short-term Mongolian gerbil infection model.
abstract: Few virulence determinants of Helicobacter pylori have been tested in vivo. We conducted this study to establish an animal model for their screening. Six-week-old male Mongolian gerbils were inoculated with wild-type H. pylori (TN2) or its isogenic mutant with deletion of cagE (TN2DeltacagE), total cag pathogenicity island (TN2DeltacagPAI), HP0499 (TN2DeltaHP499), or HP0638 (TN2DeltaHP638) (n=5 each). The animals were killed 3 weeks later, and the density of bacteria and the degree of inflammation in the stomach were compared. Infection was established in all animals except those inoculated with TN2DeltaHP638. TN2 and TN2DeltaHP499, but not TN2DeltacagE and TN2Deltacag PAI, induced intense inflammation, although the densities of bacteria were similar. The Mongolian gerbil model was useful for the screening of virulence determinants in vivo, which confirmed the importance of cag PAI while questioning that of HP0499.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo 113-8655, Japan. akanuma-2im@h.u-tokyo.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Akanuma
authors.forename: Shin
authors.initials: S
authors.lastname: Maeda
authors.forename: Keiji
authors.initials: K
authors.lastname: Ogura
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Mitsuno
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hirata
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Ikenoue
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Otsuka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamaji
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Yoshida
authors.forename: Takao
authors.initials: T
authors.lastname: Kawabe
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shiratori
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000633:toxicity*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000209:etiology*; D005849:Gerbillinae; D016480:Helicobacter pylori / Q000472:pathogenicity*; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D016133:Polymerase Chain Reaction; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D016209:Interleukin-8; C103937:PicB protein, Helicobacter pylori