title: Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography.
abstract: A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH2PO4-acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (<4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (Haliotis rufescens) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2+/-0.9% extraction efficiency.
authors.affiliation: Department of Environmental Toxicology, University of California, Davis 95616, USA. mrviant@ucdavis.edu
authors.forename: M R
authors.initials: MR
authors.lastname: Viant
authors.forename: E S
authors.initials: ES
authors.lastname: Rosenblum
authors.forename: R S
authors.initials: RS
authors.lastname: Tjeerdema
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008974:Mollusca / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D009943:Organophosphorus Compounds; C015441:phospho-L-arginine; D001120:Arginine