A blocking ELISA with improved sensitivity for the detection of passively acquired maternal antibodies to BHV-1.
Four serological tests were evaluated for their ability to detect passively acquired maternal antibodies to Bovine herpesvirus 1. A blocking enzyme-linked immunosorbent assay demonstrated superior sensitivity in the detection of such antibodies in calves up to 9-11 months old, versus calves up to 7 months old for other tests.
Cho
Hyun Ju
HJ
Canadian Food Inspection Agency-Lethbridge Laboratory, Animal Diseases Research Institute, P.O. Box 640, Lethbridge, Alberta T1J 3Z4.
Entz
Susan C
SC
Green
G Tom
GT
Jordan
Lorne T
LT
D000367:Age Factors; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology* / Q000302:isolation & purification; D007112:Immunity, Maternally-Acquired; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral