Codon 249 mutations of p53 gene in non-neoplastic liver tissues.
AIM:To study the significance of p53 gene in hepatocarcinogenesis through analyzing codon 249 mutations of p53 gene in non-neoplastic liver tissues.METHODS:Codon 249 mutation was detected using single-stranded conformational polymorphism analysis and allele-specific PCR in liver tissues from 10 cases of chronic hepatitis, 5 cases of cirrhosis and 20 cases of HCCs.RESULTS:The detection rate of codon 249 mutation in chronic hepatitis, cirrhosis and pericancerous tissues was 70% (7/10), 100% (5/5) and 70% (14/20), respectively by AS-PCR. These mutations could not be detected by SSCP analysis. The detection rates were 65% (13/20) and 45% (9/20) in cancerous tissues by AS-PCR and SSCP analysis.CONCLUSION:Codon 249 mutations of p53 gene were very popular in non-neoplastic liver tissues though the number of those mutant cells was only in subsection. Those mutations in cancerous tissues might take place in the stage before the formation of tumor.
Peng
Xiao-Mou
XM
Yao
Chun-Lan
CL
Chen
Xue-Juan
XJ
Peng
Wen-Wei
WW
Gao
Zhi-Liang
ZL