Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells.
This study was performed to characterize human thrombospondin 2 (TSP2). TSP2 has recently attracted attention as an endogenous negative regulator of angiogenesis in tumorigenesis. We cloned and transfected human TSP2 cDNA into the human colon cancer cell line SW-480. Stable transfectants (TSP2-1, TSP2-6) overexpressing TSP2 were established. Growth characteristics of TSP2-transfectants were investigated in vitro and in vivo. TSP2-transfectants showed similar growth properties to vector-transfectants and wild-type SW-480 cells. The overexpression of transfected human TSP2 cDNA did not affect proliferation of SW-480 cells. When the conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited. These results suggested that human TSP2 is a potential inhibitor of angiogenesis.
Tomii
Yasushi
Y
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Kamochi
Junichiro
J
Yamazaki
Hitoshi
H
Sawa
Nobuko
N
Tokunaga
Tetsuji
T
Ohnishi
Yasuyuki
Y
Kijima
Hiroshi
H
Ueyama
Yoshito
Y
Tamaoki
Norikazu
N
Nakamura
Masato
M
D020533:Angiogenesis Inhibitors / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000098:blood supply; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019699:Thrombospondins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D000099262:Thrombospondin 2
D020533:Angiogenesis Inhibitors; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D019699:Thrombospondins; D000099262:Thrombospondin 2