Comparison of the antigenicity of protoscoleces and microvesicles of Echinococcus multilocularis prepared from rats.
Rats are known to be relatively resistant to infection with Echinococcus multilocularis. However, when rats are inoculated with the parasite tissues, E. multilocularis proliferates slowly at first but after 6 months the cysts increase in size considerably and contain large numbers of protoscoleces. As rats survive for 18 months or longer, approximately 100 ml of packed protoscoleces can be produced from each rat. A comparison of the antigenicity of the protoscoleces and microvesicles by immunoblot methods showed that both Em18 and Em16 are shared components between both protoscoleces and microvesicles, although the latter have some additional antigenic components. In antigens prepared from protoscoleces, the banding patterns around Em18 were much simpler than those from microvesicles. Therefore, for serodiagnosis of E. multilocularis, antigens should be carefully prepared from protoscoleces rather than microvesicles from the rat.
Ito
A
A
Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan. akiraito@asahikawa-med.ac.jp
Kanazawa
T
T
Nakao
M
M
Sako
Y
Y
Ishikawa
Y
Y
Nakaya
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D018533:Biomass; D004446:Echinococcus / Q000254:growth & development / Q000276:immunology*; D005260:Female; D015151:Immunoblotting; D051379:Mice; D008813:Mice, Inbred ICR; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D015415:Biomarkers; C117889:Em18 antigen