Distribution of the receptor for advanced glycation end products gene polymorphisms in patients with chronic periodontitis: a preliminary study.
BACKGROUND
Periodontal diseases are viewed today as multifactorial problems initiated and sustained by bacteria but significantly modified by the body's response to bacterial plaque. A recent study suggested that receptor for advanced glycation end products (RAGE) could be involved in the pathophysiology of periodontitis. The aim of this study was to investigate a possible association of 3 common polymorphisms in the RAGE gene with chronic periodontitis.


METHODS
We studied 101 Caucasian patients with chronic periodontitis together with 162 orally healthy subjects. Three polymorphisms, one in intron 7 (1704G/T), second in intron 8 (2184A/G), and the third in exon 3 (G82S) of the RAGE gene, were investigated by polymerase chain reaction methods (PCR) with subsequent enzymatic restriction with Bfal, BsmFI, or Alu Il, respectively.


RESULTS
A statistically significant difference in allele frequencies between patients and the reference group was found for intron variant 1704G/T (P= 0.02, Pcorr >0.05). There was no significant difference in genotype or allele frequency distributions between groups for intron variant 2184A/G or for the exon variant exchanging amino acid Gly for Ser at position 82 (G82S).


CONCLUSIONS
We can speculate that susceptibility to the development of chronic periodontitis could be influenced by the 1704G/T polymorphism of the RAGE gene, independently of diabetes.
Hollá
L I
LI
Institute of Pathological Physiology, Medical Faculty, Masaryk University Brno, Czech Republic. holla@med.muni.cz
Kanková
K
K
Fassmann
A
A
Bucková
D
D
Halabala
T
T
Znojil
V
V
Vanĕk
J
J
D000328:Adult; D000483:Alleles; D019943:Amino Acid Substitution; D016009:Chi-Square Distribution; D002908:Chronic Disease; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D017127:Glycation End Products, Advanced / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics*; D018709:Statistics, Nonparametric
D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic