[Expression of surviving gene and its relationship with expression of P53, c-myc, k-ras proteins in non-small-cell lung cancer].
OBJECTIVE
To study the expression of surviving and its relationship with expression of P53, c-myc, k-ras in non-small-cell lung cancer (NSCLC).


METHODS
Expression of the surviving mRNA was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) in 76 NSCLC tumor samples, 20 benign phymatoid lesion and 21 adjacent normal lung tissue samples. Immunohistochemical assay was to detect the expression of P53, c-myc, k-ras proteins.


RESULTS
Expression of surviving gene was detected in a significantly greater proportion of NSCLC (61%) than phymatoid lesion (30%) and adjacent normal lung tissue (19%) (P < 0.001). There was no relationship between surviving gene expression and histologic subtype, differentiation, TNM stages, or lymph node metastases. The expression of surviving gene correlated with P53 or c-myc expression, but not k-ras expression.


CONCLUSIONS
(1) The up-regulation expression of surviving gene in NSCLC suggested that surviving may play a role in the pathway of carcinogenesis and surviving may be identified as a potential therapeutic target in NSCLC. (2) surviving, de-activation of antioncogene P53 and up-regulation of oncogene c-myc might play synergetic roles in the process of carcinogenesis of NSCLC. But surviving and k-ras may be independently involved in the pathogenesis of lung cancer.
Wang
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Research Laboratory of Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Chen
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Zhang
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D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008869:Microtubule-Associated Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis*; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D015854:Up-Regulation
C107489:BIRC5 protein, human; D002868:Chromosomal Proteins, Non-Histone; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D000077022:Survivin; D016159:Tumor Suppressor Protein p53; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)