title: Stabilization of RNA strands in protein synthesis by type I procollagen C-proteinase enhancer protein, a potential RNA-binding protein, in hepatic stellate cells.
abstract: Type I procollagen C-proteinase enhancer (PCPE) exists in hepatic stellate cells (HSCs) which can produce collagen. The deduced amino acid sequence of PCPE contains motifs specific for RNA-binding proteins. The effect of PCPE on the syntheses of collagen and noncollagenous protein was studied using an HSC clone derived from cirrhotic rat liver. When the cells were cultured in the presence of an antisense oligonucleotide (AS) against PCPE mRNA, the synthesis of noncollagenous protein as well as collagen was reduced compared to the cells cultured with addition of a nonsense oligonucleotide (NS). The extent of the reduction was similar in both syntheses. The total RNA content of the AS-treated cells and NS-treated cells did not differ. In the presence of actinomycin D, however, such total RNA content was decreased more rapidly in the AS-treated cells than in the NS-treated cells. PCPE may be involved in stabilization of RNA strands in noncollagenous protein synthesis as well as collagen synthesis.
authors.affiliation: Department of Gastroentelorogy, University of Tokyo, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsui
authors.forename: Mikio
authors.initials: M
authors.lastname: Yanase
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tomiya
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ikeda
authors.forename: Kenji
authors.initials: K
authors.lastname: Fujiwara
authors.forename: Itsuro
authors.initials: I
authors.lastname: Ogata
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002999:Clone Cells; D003094:Collagen / Q000096:biosynthesis; D024042:Collagen Type I / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D051381:Rats
chemical_list: D024042:Collagen Type I; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D016376:Oligonucleotides, Antisense; C491554:Pcolce protein, rat; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D003609:Dactinomycin; D012313:RNA; D003094:Collagen