title: Interaction between Arabidopsis heat shock transcription factor 1 and 70 kDa heat shock proteins.
abstract: The activity of the Arabidopsis heat shock transcription factor (HSF) is repressed at normal conditions but activated by cellular stresses. Circumstantial evidence suggests that HSP70 may function as a negative feedback regulator of HSF activity. Here the interaction between HSF and HSP70 is reported using electrophoretic mobility shift and yeast two-hybrid assays. Subdomain mapping indicates an interaction of the activation domain and DNA-binding domain of HSF1 with HSP70.
authors.affiliation: Universität Tübingen, ZMBP-Zentrum für Molekularbiologie der Pflanzen, Allgemeine Genetik, Auf der Morgenstelle 28, D-72076 Tübingen, Germany.
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Kim
authors.forename: Fritz
authors.initials: F
authors.lastname: Schöffl
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D050883:HSC70 Heat-Shock Proteins; C000620151:HSF1 protein, Arabidopsis; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins; D014157:Transcription Factors