Molecular genotyping of the mouse scid allele.
Severe combined immunodeficiency (SCID) mice, which lack mature B- and T-cells, provide an important system for studies on immunity and disease. However, since the scid mutation is a single T-to-A transversion, genotypic analysis can be problematic. We have developed a rapid and simple sequence-based analysis that provides identification of both the scid and the wild-type (+) allele. This allows unequivocal identification of scid/scid, scid/+ and +/+ individuals. The method is of greatest utility for discerning scid/+ from +/+ animals during genetic complementation studies, but may be of value for routine SCID colony control as well.
Sealey
Amy L
AL
Veterinary Molecular Biology, Marsh Laboratories, Montana State University, Bozeman, MT 59717, USA.
Hobbs
Nicole K
NK
Schmidt
Edward E
EE
D000483:Alleles; D000818:Animals; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D051379:Mice; D016513:Mice, SCID; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017421:Sequence Analysis / Q000379:methods*
D004268:DNA-Binding Proteins; D051747:DNA-Activated Protein Kinase; D017346:Protein Serine-Threonine Kinases