title: Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment.
abstract: Type I allergy is an immunoglobulin E (IgE)-mediated hypersensitivity disease affecting more than 25% of the population. Currently, diagnosis of allergy is performed by provocation testing and IgE serology using allergen extracts. This process defines allergen-containing sources but cannot identify the disease-eliciting allergenic molecules. We have applied microarray technology to develop a miniaturized allergy test containing 94 purified allergen molecules that represent the most common allergen sources. The allergen microarray allows the determination and monitoring of allergic patients' IgE reactivity profiles to large numbers of disease-causing allergens by using single measurements and minute amounts of serum. This method may change established practice in allergy diagnosis, prevention, and therapy. In addition, microarrayed antigens may be applied to the diagnosis of autoimmune and infectious diseases.
authors.affiliation: VBC-GENOMICS, A-1030 Vienna, Austria.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hiller
authors.forename: Sylvia
authors.initials: S
authors.lastname: Laffer
authors.forename: Christian
authors.initials: C
authors.lastname: Harwanegg
authors.forename: Martin
authors.initials: M
authors.lastname: Huber
authors.forename: Wolfgang M
authors.initials: WM
authors.lastname: Schmidt
authors.forename: Anna
authors.initials: A
authors.lastname: Twardosz
authors.forename: Bianca
authors.initials: B
authors.lastname: Barletta
authors.forename: Wolf M
authors.initials: WM
authors.lastname: Becker
authors.forename: Kurt
authors.initials: K
authors.lastname: Blaser
authors.forename: Heimo
authors.initials: H
authors.lastname: Breiteneder
authors.forename: Martin
authors.initials: M
authors.lastname: Chapman
authors.forename: Reto
authors.initials: R
authors.lastname: Crameri
authors.forename: Michael
authors.initials: M
authors.lastname: Duchêne
authors.forename: Fatima
authors.initials: F
authors.lastname: Ferreira
authors.forename: Helmut
authors.initials: H
authors.lastname: Fiebig
authors.forename: Karin
authors.initials: K
authors.lastname: Hoffmann-Sommergruber
authors.forename: Te Piao
authors.initials: TP
authors.lastname: King
authors.forename: Tamara
authors.initials: T
authors.lastname: Kleber-Janke
authors.forename: Viswanath P
authors.initials: VP
authors.lastname: Kurup
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Lehrer
authors.forename: Jonas
authors.initials: J
authors.lastname: Lidholm
authors.forename: Ulrich
authors.initials: U
authors.lastname: Müller
authors.forename: Carlo
authors.initials: C
authors.lastname: Pini
authors.forename: Gerald
authors.initials: G
authors.lastname: Reese
authors.forename: Otto
authors.initials: O
authors.lastname: Scheiner
authors.forename: Annika
authors.initials: A
authors.lastname: Scheynius
authors.forename: Horng-Der
authors.initials: HD
authors.lastname: Shen
authors.forename: Susanne
authors.initials: S
authors.lastname: Spitzauer
authors.forename: Roland
authors.initials: R
authors.lastname: Suck
authors.forename: Ines
authors.initials: I
authors.lastname: Swoboda
authors.forename: Wayne
authors.initials: W
authors.lastname: Thomas
authors.forename: Raffaela
authors.initials: R
authors.lastname: Tinghino
authors.forename: Marianne
authors.initials: M
authors.lastname: Van Hage-Hamsten
authors.forename: Tuomas
authors.initials: T
authors.lastname: Virtanen
authors.forename: Dietrich
authors.initials: D
authors.lastname: Kraft
authors.forename: Manfred W
authors.initials: MW
authors.lastname: Müller
authors.forename: Rudolf
authors.initials: R
authors.lastname: Valenta
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D007073:Immunoglobulin E / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000485:Allergens; D011994:Recombinant Proteins; D007073:Immunoglobulin E