title: The effect of bacterial endotoxin on the early tensile strength of healing surgical wounds.
abstract: Wound healing in the oral cavity occurs in a bacteria-rich environment, which may affect its outcome. Furthermore, it takes place where forces are frequently applied to the healing tissue. The effect of bacterial endotoxin on the development of tensile strength in healing wounds was studied using surgical skin wounds in rats as a model. Collagen membranes soaked with 0.01 microg of bacterial endotoxin were inserted into surgical skin wounds, and their effect was studied on days 6 and 10. Membranes with no endotoxin served as controls. Endotoxin inhibited the early development of tensile strength in 6 days, healing wounds by 38%, whereas the collagen membrane alone had no effect. Dexamethasone (0.5 mg/kg every 72 h) had a suppressive effect on the development of tensile strength in healing noncontaminated wounds, but not in those containing bacterial endotoxin. These results suggest that bacterial endotoxin may interfere with the early healing of wounds. Understanding the mechanisms of this inhibition may result in treatments that will allow this response to be faster and more reproducible.
authors.affiliation: Department of Oral Biology, The Goldschleger School of Dental Medicine, Tel Aviv University, Ramat Aviv, Israel.
authors.forename: Zvi
authors.initials: Z
authors.lastname: Metzger
authors.forename: Daniel
authors.initials: D
authors.lastname: Nitzan
authors.forename: Sandu
authors.initials: S
authors.lastname: Pitaru
authors.forename: Tamar
authors.initials: T
authors.lastname: Brosh
authors.forename: Shlomo
authors.initials: S
authors.lastname: Teicher
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D024042:Collagen Type I; D062109:Dermatologic Surgical Procedures; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004731:Endotoxins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004926:Escherichia coli; D005500:Follow-Up Studies; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000503:physiopathology; D013223:Statistics as Topic; D013529:Surgical Wound Dehiscence / Q000503:physiopathology; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D024042:Collagen Type I; D004731:Endotoxins; D008070:Lipopolysaccharides; D008567:Membranes, Artificial; D003907:Dexamethasone