title: Effect of growth conditions on the synthesis of terminal oxidases in Methylobacillus flagellatus KT.
abstract: Membranes of the obligate methylotroph Methylobacillus flagellatus KT contained hemes B, O, and C and cytochromes b, o, and c both in batch and in continuous cultures. Neither heme A nor heme D was detected in the membranes. The cytochromes o and bb were the main components reversibly binding carbon monoxide (CO) in the terminal part of the respiratory chain. The alpha-region and especially the alpha-peaks at 568 and 573 nm and the alpha-troughs at 586 and 592 on the CO-difference spectra were diagnostic for the cytochromes o and bb, respectively. The cytochrome o content increased up to 1.8 times upon increasing the dilution rate of the culture from 0.15 to 0.55 h(-1) under methanol limitation. By contrast, the level of the CO-binding cytochrome bb was not affected by methanol concentration but its content increased up to 1.9 times when the level of oxygen decreased from 95 to 21 microM under the constant dilution rate (mu = 0.55 h(-1)). The maximum ratio between the cytochromes o and bb reached 2 during continuous cultivation under methanol-limited conditions (mu = 0.55 h(-1)), whereas the minimum ratio between them was about 0.7 during batch cultivation at stationary phase of growth. The synthesis of the CO-binding cytochrome bb but not of the cytochrome o in M. flagellatus KT was assumed to depend on the ambient redox potential of the medium. The cytochrome o synthesis was supposed to depend on the transmembrane gradient of protons (Delta(mu)H+).
authors.affiliation: Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow, 119899, Russia. muntyan@genebee.msu.su
authors.forename: Maria S
authors.initials: MS
authors.lastname: Muntyan
authors.forename: Tatiana Yu
authors.initials: TY
authors.lastname: Dinarieva
authors.forename: Mark V
authors.initials: MV
authors.lastname: Baev
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Netrusov
mesh_terms: D018929:Cell Culture Techniques; D003470:Culture Media / Q000378:metabolism*; D003580:Cytochromes / Q000737:chemistry; D006418:Heme / Q000737:chemistry; D020631:Methylobacillus / Q000201:enzymology* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*
chemical_list: D003470:Culture Media; D003580:Cytochromes; D006418:Heme; D010088:Oxidoreductases