Investigation of a candidate gene, environment, and G x E interaction using case-control and case-parent study designs.
We investigated the independent contributions of a candidate gene and an environmental factor, and the presence of gene x environment (G x E) interaction, in the etiology of a disease in the Genetic Analysis Workshop (GAW) 12 problem 2 simulated data using a two-stage approach utilizing both case-control and case-parent study designs. Using the case-control design, several SNPs within candidate gene 1 (CG1) and environmental factor 1 (dichotomized using the 75th percentile as a cut-off) (EXP) were independently associated with disease status, in models adjusted for age and sex. We found evidence of gene x environment (G x E) interaction between EXP and two single-nucleotide polymorphisms (SNPs) within CG1 using the case-control design. Using the case-parent study design in the same population, we detected association between SNPs within CG1 and disease, but no G x E interaction was detected.
Norris
J M
JM
Univ. of Colorado Health Sci. Center, Department of Preventive Medicine and Biometrics, 4200 East Ninth Ave., Box C245, Denver, CO 80262, USA.
Selinger-Leneman
H
H
Génin
E
E
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D016013:Likelihood Functions; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics