title: Analysis of non-porcine isolates of Actinobacillus suis.
abstract: Twenty-four Actinobacillus suis isolates obtained from several species of non-porcine mammals were compared to the representative porcine strains, ATCC 15557 (serotype O1) and H89-1173 (serotype O2), by O serotyping, DNA fingerprinting, PCR amplification of apxICA, apxIICA and apxIIICA toxin genes and by rrs (16S rRNA) gene sequencing. Only two strains, both equine, reacted with O1 antiserum while two others, one canine and the other feline, reacted with O2 antiserum. One equine strain reacted weakly with both antisera. No amplification of apx genes was found with the non-porcine O1 or the "not O1/O2" strains but amplification of the apxICA and apxIICA genes was observed with the two O2 strains. In addition, these two O2 strains had both BamHI and BglII fingerprints that were very similar to the porcine O2 reference strain, H89-1173 and rrs gene sequences that were identical to the A. suis reference strain ATCC 15557. Taken together, these data suggest that although many non-porcine A. suis isolates are not A. suis (sensu stricto), some isolates are genotypically as well as phenotypically similar to A. suis.
authors.affiliation: Département de Pathologie et de Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe, Qué., Canada J2S 7C6.
authors.forename: Marie Eve
authors.initials: ME
authors.lastname: Jeannotte
authors.forename: Durda
authors.initials: D
authors.lastname: Slavić
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Janet I
authors.initials: JI
authors.lastname: MacInnes
mesh_terms: D000188:Actinobacillus / Q000145:classification / Q000235:genetics*; D000189:Actinobacillus Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics*; D002415:Cats; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D004285:Dogs; D005784:Gene Amplification; D005798:Genes, Bacterial; D005838:Genotype; D006460:Hemolysin Proteins; D006736:Horses; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011817:Rabbits; D012703:Serotyping / Q000379:methods / Q000662:veterinary; D013045:Species Specificity; D013552:Swine
chemical_list: C085361:ApxII toxin, bacteria; C079721:ApxIII toxin protein, Actinobacillus; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; C087744:ApxI toxin, Bacteria