title: Combinatorial RNAi: a method for evaluating the functions of gene families in Drosophila.
abstract: Individual members of gene families often have partially redundant functions during nervous system development, making conventional genetic analysis problematic. Here we review experiments showing that several genes can be silenced together by injection of double-stranded RNAs into wild-type Drosophila embryos. By dye-labeling single neuroblasts in injected embryos, the effects of multigene silencing on individual CNS axon pathways can now be examined.
authors.affiliation: Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. Aloisia.T.Schmid@am.pnu.com
authors.forename: Aloisia
authors.initials: A
authors.lastname: Schmid
authors.forename: Benno
authors.initials: B
authors.lastname: Schindelholz
authors.forename: Kai
authors.initials: K
authors.lastname: Zinn
mesh_terms: D000818:Animals; D004330:Drosophila / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D008845:Microinjections; D005810:Multigene Family / Q000187:drug effects / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D012330:RNA, Double-Stranded