title: Resolving isomeric peptide mixtures: a combined HPLC/ion mobility-TOFMS analysis of a 4000-component combinatorial library.
abstract: A reversed-phase high-performance liquid chromatography (HPLC) separation approach has been combined with ion mobility/time-of-flight (TOF) mass spectrometry in order to characterize a combinatorial peptide library designed to contain 4000 peptides of the general form NH2-Xxx-Xxx-XXX-CO2H, NH2-Ala-Xxx-Xxx-Xxx-CO2H, NH2-Ser-Ala-Xxx-Xxx-Xxx-CO2H and NH2-Leu-Ser-Ala-Xxx-Xxx-Xxx-CO2H (where Xxx represents a randomization over 10 different amino acids: Ala, Arg, Asp, Glu, Gly, Leu, Lys, Phe, Ser, and Val). Addition of the gas-phase mobility separation between the HPLC separation and TOF measurement dimensions makes it possible to resolve many peptide isomers that have identical retention times (and masses).
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405, USA.
authors.forename: BarnesCatherineA
authors.initials: B
authors.lastname: Srebalus
authors.forename: Amy E
authors.initials: AE
authors.lastname: Hilderbrand
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Valentine
authors.forename: David E
authors.initials: DE
authors.lastname: Clemmer
mesh_terms: D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D019151:Peptide Library; D010455:Peptides