The expression of Bok is regulated by serum in HC11 mammary epithelial cells.
Epithelial cells within the mammary gland undergo apoptosis during weaning. To determine the expression of Bok mRNA (a member of the pro-apoptotic Bcl-2 family) in the mammary gland and its regulation, we examined the expression of the Bok transcript in the mouse mammary gland and HC11 mammary epithelial cells in culture through RT-PCR. The Bok mRNA expression was found in the mammary gland. The expression of the Bok mRNA level was induced through serum starvation and overexpression of Bok induced apoptosis in HC11 cells in culture. These results indicate that the expression of Bok mRNA in the mammary gland is regulated through serum starvation. It also may be related to the mammary involution.
Ha
S H
SH
Department of Animal Science and Technology, School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.
Lee
S R
SR
Lee
T H
TH
Kim
Y M
YM
Baik
M G
MG
Choi
Y J
YJ
D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; D008565:Membrane Proteins; D012333:RNA, Messenger