title: Detection of St. Louis encephalitis and western equine encephalomyelitis RNA in mosquitoes tested without maintenance of a cold chain.
abstract: Western equine encephalomyelitis and St. Louis encephalitis viral RNA can be detected 20 days after death of infected Culex tarsalis in the absence of a cold chain. Viral RNA was detected with the reverse transcription-polymerase chain reaction in mosquitoes infected either parenterally or perorally in the laboratory and then killed and held for up to 20 days at 27 degrees C. Cell culture assay and in situ enzyme immunoassay did not detect infectious virus in the same mosquitoes.
authors.affiliation: The Arbovirus Laboratories, New York State Department of Health, Slingerlands 12159, USA.
authors.forename: L D
authors.initials: LD
authors.lastname: Kramer
authors.forename: R E
authors.initials: RE
authors.lastname: Chiles
authors.forename: T D
authors.initials: TD
authors.lastname: Do
authors.forename: H M
authors.initials: HM
authors.lastname: Fallah
mesh_terms: D000818:Animals; D003080:Cold Temperature; D003465:Culex / Q000821:virology*; D004665:Encephalitis Virus, St. Louis / Q000235:genetics*; D004667:Encephalitis Virus, Western Equine / Q000235:genetics*; D005260:Female; D007303:Insect Vectors / Q000821:virology; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral