title: Effect of avocado and soybean unsaponifiables on gelatinase A (MMP-2), stromelysin 1 (MMP-3), and tissue inhibitors of matrix metalloproteinase (TIMP- 1 and TIMP-2) secretion by human fibroblasts in culture.
abstract: BACKGROUND
  In inflamed periodontal tissues, gingival fibroblasts are able to express matrix metalloproteinases (MMPs) and their natural inhibitors, tissue inhibitors of matrix metalloproteinases (TIMPs). They can also respond to growth factors and cytokines. In this study, the in vitro effects of avocado and soybean unsaponifiable residues (ASU), their fractions (avocado unsaponifiable [ASF] or soy unsaponifiable [SSF]) on MMP-2 and MMP-3, and the activity and secretion of their inhibitors TIMP-1 and TIMP-2 were investigated using cultured human gingival fibroblasts.
  
  
  METHODS
  Gingival fibroblasts were cultured for 72 hours with ASU, ASF, and SSF at concentrations of 0. 1, 0.5, 2.5, 5, and 10 microgram/ml of culture medium, after pretreatment or no pretreatment for 1 hour with interleukin-1beta (IL-1beta). MMP-2 and MMP-3 were detected and quantified in the culture media after zymography and image analysis. TIMP-1, TIMP-2, MMP-2, and MMP-3 were also evidenced by dot blotting and quantified by image analysis.
  
  
  RESULTS
  In the absence of IL-1beta, a slight decrease in the secretion of MMP-2 was observed with lower doses of ASU, ASF, and SSF. The decrease of MMP-3 secretion was clearly marked with all fractions especially at low concentrations (0.1 and 2.5 microgram/ml). A slight decrease in TIMP-2 secretion was seen for low doses of ASU, ASF, and SSF, while a small increase was seen at higher concentrations. Concerning TIMP-1, no significant variation was observed in culture medium for low concentrations, and a decrease was noted for 5 and 10 microgram/ml of ASU, ASF, and SSF. As anticipated, IL-1beta induced a marked release of MMP-2, MMP-3, and TIMP-1, but no variation for TIMP-2 was seen. ASU, ASF, and SSF reversed the IL-1beta effect on gingival fibroblasts for MMP-2 and MMP-3, particularly with doses varying from 0.1 to 2.5 microgram/ml and for TIMP-1, particularly with doses varying from 2.5 to 10 microgram/ml.
  
  
  CONCLUSIONS
  These findings suggest a potential role for avocado and soy unsaponifiable extracts to prevent the deleterious effects of IL-1beta that occur during periodontal diseases.
authors.affiliation: Laboratory of Physiopathology of Non-Mineralized Tissues, Faculty of Dental Surgery, University of Paris V, Montrouge, France.
authors.forename: C
authors.initials: C
authors.lastname: Kut-Lasserre
authors.forename: C C
authors.initials: CC
authors.lastname: Miller
authors.forename: A L
authors.initials: AL
authors.lastname: Ejeil
authors.forename: B
authors.initials: B
authors.lastname: Gogly
authors.forename: M
authors.initials: M
authors.lastname: Dridi
authors.forename: N
authors.initials: N
authors.lastname: Piccardi
authors.forename: B
authors.initials: B
authors.lastname: Guillou
authors.forename: B
authors.initials: B
authors.lastname: Pellat
authors.forename: G
authors.initials: G
authors.lastname: Godeau
mesh_terms: D000704:Analysis of Variance; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000201:enzymology; D005881:Gingiva / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D015151:Immunoblotting; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010518:Periodontitis / Q000188:drug therapy* / Q000201:enzymology; D027421:Persea / Q000737:chemistry; D008517:Phytotherapy; D010938:Plant Oils / Q000627:therapeutic use*; D013024:Soybean Oil / Q000627:therapeutic use; D013025:Glycine max / Q000737:chemistry; D019714:Tissue Inhibitor of Metalloproteinases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
chemical_list: D007375:Interleukin-1; D010938:Plant Oils; D019714:Tissue Inhibitor of Metalloproteinases; D013024:Soybean Oil; D008666:Metalloendopeptidases