Antioxidant and immunostimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps sp.
Cordyceps is negative for its many biological activities and a tonic for restoring vital functions in traditional Chinese medicine. In an effort to evaluate the pharmacological effects, including the antiaging effect of the fruiting bodies of the cultivated Paecilomyces japonica fungus, a new type of Cordyceps sp. was investigated. This investigation was focused on ultimately revealing its biologically active principles, its effects on free-radical scavenging enzymes, lipid peroxidation, as well as its immunological functions. As a result, both water and methanol extracts were found to cause not only significant increases in rat liver cytosolic SOD, catalase, and GSEH-px activities, but also a significant decrease in MDA production in TBA reactant assay in rats. The extracts also showed immunostimulating activity as measured by carbon clearance, weight-loaded forced swimming performances, and immobilizing stress in mice. Using bioassay-guided systematic fractionation of the extracts, two pure compounds were isolated as active principles from low molecular-weight fraction, a protein-bound polysaccharide was isolated that showed a marked increase in the liver enzyme activities, as well as a significant inhibition of lipid peroxidation.
Shin
K H
KH
Natural Products Research Institute, Seoul National University, Korea. khshin@plaza.snu.ac.kr
Lim
S S
SS
Lee
S H
SH
Lee
Y S
YS
Cho
S Y
SY
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D012831:Bombyx / Q000382:microbiology; D002244:Carbon / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy; D002374:Catalase / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D004875:Ergosterol / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005609:Free Radicals; D005688:Galactosamine / Q000032:analysis; D007103:Immobilization / Q000009:adverse effects; D007814:Larva / Q000382:microbiology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008516:Medicine, Chinese Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010143:Paecilomyces / Q000737:chemistry* / Q000145:classification; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000188:drug therapy / Q000276:immunology / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013550:Swimming
C447157:1,6-dimethylmannitol; D000276:Adjuvants, Immunologic; D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D011134:Polysaccharides; D008353:Mannitol; D008315:Malondialdehyde; D002244:Carbon; D005688:Galactosamine; D002374:Catalase; D013482:Superoxide Dismutase; D004875:Ergosterol