title: Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking.
abstract: In order to develop a scaffolding material for tissue regeneration, porous matrices containing collagen and hyaluronic acid were fabricated by freeze drying at -20 degrees C, -70 degrees C or -196 degrees C. The fabricated porous membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1-100 mM concentrations for enhancing mechanical stability of the composite matrix. Scanning electron microscope (SEM) views of the matrices demonstrated that the matrices obtained before cross-linking process had interconnected pores with mean diameters of 40, 90 or 230 microm and porosity of 58-66% according to the freezing temperature, and also the porous structures after cross-linking process were retained. The swelling test and IR spectroscopic measurement of different cross-linked membranes were carried out as a measure of the extent of cross-linking. The swelling behavior of cross-linked membranes showed no significant differences as cross-linking degree increased. FT-IR spectra showed the increase of the intensity of the absorbencies at amide bonds (1655, 1546, 1458 cm(-1)) compared to that of CH bond (2930 cm(-1)). In enzymatic degradation test, EDC treated membranes showed significant enhancement of the resistance to collagenase activity in comparison with 0.625% glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity.
authors.affiliation: Department of Medical Engineering, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Si-Nae
authors.initials: SN
authors.lastname: Park
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Hea Ok
authors.initials: HO
authors.lastname: Kim
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Song
authors.forename: Hwal
authors.initials: H
authors.lastname: Suh
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D001673:Biodegradation, Environmental; D002417:Cattle; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D003432:Cross-Linking Reagents; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000031:analogs & derivatives*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; C041829:1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide methiodide; D006820:Hyaluronic Acid; D003094:Collagen; D005022:Ethyldimethylaminopropyl Carbodiimide