title: Prediction of unidentified human genes on the basis of sequence similarity to novel cDNAs from cynomolgus monkey brain.
abstract: BACKGROUND
  The complete assignment of the protein-coding regions of the human genome is a major challenge for genome biology today. We have already isolated many hitherto unknown full-length cDNAs as orthologs of unidentified human genes from cDNA libraries of the cynomolgus monkey (Macaca fascicularis) brain (parietal lobe and cerebellum). In this study, we used cDNA libraries of three other parts of the brain (frontal lobe, temporal lobe and medulla oblongata) to isolate novel full-length cDNAs.
  
  
  RESULTS
  The entire sequences of novel cDNAs of the cynomolgus monkey were determined, and the orthologous human cDNA sequences were predicted from the human genome sequence. We predicted 29 novel human genes with putative coding regions sharing an open reading frame with the cynomolgus monkey, and we confirmed the expression of 21 pairs of genes by the reverse transcription-coupled polymerase chain reaction method. The hypothetical proteins were also functionally annotated by computer analysis.
  
  
  CONCLUSIONS
  The 29 new genes had not been discovered in recent explorations for novel genes in humans, and the ab initio method failed to predict all exons. Thus, monkey cDNA is a valuable resource for the preparation of a complete human gene catalog, which will facilitate post-genomic studies.
authors.affiliation: Division of Genetic Resources, National Institute of Infectious Diseases, 1-23-1 Toyama-cho, Shinjuku-ku, 162-8640, Japan. osada@nih.go.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Osada
authors.forename: Munetomo
authors.initials: M
authors.lastname: Hida
authors.forename: Jun
authors.initials: J
authors.lastname: Kusuda
authors.forename: Reiko
authors.initials: R
authors.lastname: Tanuma
authors.forename: Makoto
authors.initials: M
authors.lastname: Hirata
authors.forename: Momoki
authors.initials: M
authors.lastname: Hirai
authors.forename: Keiji
authors.initials: K
authors.lastname: Terao
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D018076:DNA, Complementary; D012313:RNA