title: [The study of beta-2 adrenergic receptors gene transferred into cardiomyocytes by adenovirus vectors contain different promoter].
abstract: OBJECTIVE
  To observe human beta(2) adrenergic receptors (beta(2)-AR) gene expression in cardiomyocytes transfected by adenoviral vector contain cmv or mlc-2v promoter.
  
  
  METHODS
  Two adenoviral vectors (Adcmv beta(2) AR, Admlc beta(2) AR) were constructed, in which beta(2)-AR gene is under control of either the ventricle-specific myosin light chain-2 (mlc-2v) or the cmv promoter. The cultured neonate rat ventricular myocytes were infected by two adenoviral vectors, and the beta(2)-AR expression of on cultured neonate rat ventricular myocytes and their ability to potentiate beta-adrenergic signaling were assessed.
  
  
  RESULTS
  The presence of beta(2)-AR mRNA was detected by RT-PCR, and the expression of the beta(2)-AR gene was demonstrated by protein immunoblots. According to a ligand binding assay, the density of beta-AR in the cardiomyocytes infected by Admlc beta(2) AR and Adcmv beta(2) AR was greater than that in the control. Moreover, the latter was higher than the former (234 +/- 6.4 fmol/mg protein vs 153 +/- 5.2 fmol/mg protein) (P < 0.01). It was demonstrated that beta(2)-AR gene significantly increased isoproterenol stimulated cAMP in cardiomyocytes.
  
  
  CONCLUSIONS
  The results above demonstrated that human beta(2)-AR gene was expressed in the cardiomyocytes infected by recombinant adenovirus contain cmv or mlc-2v promoter, but both is different.
authors.affiliation: Department of Senile Cardiology, General Hospital of PLA, Beijing 100853, China.
authors.forename: W
authors.initials: W
authors.lastname: Gao
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Pei
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D018343:Receptors, Adrenergic, beta-2; D000242:Cyclic AMP