Isoflurane aggravates the decrease of phosphatidycholine synthesis in alveolar type II cells induced by hydrogen peroxide.
The influence of isoflurane (Iso) on the synthesis and secretion of phosphatidylcholine (PC) of alveolar type II cells (AT II cells) injured by hydrogen peroxide (H2O2) was investigated. After primary culturing for 32 h, AT II cells isolated and purified from adult Sprague-Dawley rats were randomly divided into six groups: control group, 02.8 mM Iso group, 2.8 mM Iso group, 75 microM H2O2 group, 75 microM H2O2 + 0.28 mM Iso group, and 75 microM H202 + 2.8 mM Iso group. Synthesis and secretion of phosphatidylcholine (PC) were detected by 3H-choline chloride incorporation. It was found that 0.28 mM and 2.8 mM Iso significantly reduced PC synthesis compared with the control group (p <0.05, p <0.01, respectively), but not PC secretion. 75 microM H2O2 markedly decreased the synthesis and secretion of PC in AT II cells compared with the control group (p <0.01). 0.28 mM and 2.8 mM Iso aggravated the decrease of PC synthesis induced by H2O2 (p <0.05, p <0.01, respectively), but did not affect PC secretion. These findings suggest that Iso itself may inhibit the synthesis of PC of AT II cells in vitro and further damage the cells' function under peroxidation.
Yang
T
T
Department ofAnesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
Li
Y
Y
Liu
Q
Q
Tao
J
J
Wu
W
W
Huang
H
H
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D018929:Cell Culture Techniques; D004357:Drug Synergism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000096:biosynthesis / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D018685:Anesthetics, Inhalation; D010713:Phosphatidylcholines; D011663:Pulmonary Surfactants; D006861:Hydrogen Peroxide; D007530:Isoflurane