Effect of nitric oxide synthase inhibitor on the local cerebral blood flow evoked by rat somatosensory stimulation under hyperoxia.
Hyperoxia reduces the hemodynamic latency and enhances the response magnitude of the evoked local cerebral blood flow (LCBF). The objective of this study was to test the hypothesis that a change in the production of nitric oxide (NO) is involved in a unique change in evoked LCBF during hyperoxia. We measured LCBF in alpha-chloralose-anesthetized rats by laser-Doppler flowmetry. Systemic administration of the NO synthase inhibitor N(omega)-nitro-L-arginine (LNA) caused a decline in the baseline level of LCBF (P<0.01). The LNA intravenous injection during hyperoxia (hyperoxia with LNA) reduced the normalized evoked LCBF (normalization with respect to the baseline level of LCBF) in response to somatosensory stimulation by approximately 37% when compared under normal conditions (normoxia without LNA) (P<0.01), although that during normoxia (normoxia with LNA) did not cause a significant difference in the normalized evoked LCBF. The integrated neuronal activity under hyperoxia with LNA was approximately 11% lower than that under normoxia without LNA (P<0.05), although there was no significant difference in integrated neuronal activity between normoxia with LNA and normoxia without LNA. These results do not support our hypothesis and suggest the existence of another interaction mechanism involving oxygen for the enhancement of evoked LCBF under hyperoxia.
Matsuura
Tetsuya
T
Department of Radiology and Nuclear Medicine, Akita Research Institute of Brain and Blood Vessels, 6-10 Senshu-kubota machi, Akita 010-0874, Japan. matsuurat@sci.ehime-u.ac.jp
Kanno
Iwao
I
D000818:Animals; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D004594:Electrophysiology; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D018496:Hyperoxia / Q000201:enzymology / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D019335:Nitroarginine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects / Q000502:physiology
D019335:Nitroarginine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase