Meropenem stability to beta-lactamase hydrolysis and comparative in vitro activity against several beta-lactamase-producing Gram-negative strains.
The interaction between meropenem and class A, B, C and D beta-lactamases was studied by a spectrophotometric method. Class A, C and D beta-lactamases were unable to confer in vitro resistance to carbapenems. Surprisingly, several class B metallo-beta-lactamases expressed in Escherichia coli failed to confer resistance when a conventional inoculum (105 cfu/mL) was used.
Franceschini
N
N
Dipartimento di Scienze e Tecnologie Biomediche, Università dell'Aquila, Via Vetoio, 67100 L'Aquila, Italy.
Segatore
B
B
Perilli
M
M
Vessillier
S
S
Franchino
L
L
Amicosante
G
G
D004789:Enzyme Activation / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D000077731:Meropenem; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D013845:Thienamycins / Q000493:pharmacokinetics* / Q000494:pharmacology; D001618:beta-Lactamases / Q000096:biosynthesis / Q000145:classification / Q000378:metabolism*
D013845:Thienamycins; D001618:beta-Lactamases; D000077731:Meropenem