[Monitoring gene expression profile changes in endometrial cancer using cDNA microarray technology].
OBJECTIVE
To study the difference in gene expression between normal endometrial tissue and endometrial cancer tissue so as to investigate the mechanism of pathogenesis of endometrial cancer.


METHODS
The tissues from 30 patients with endometrial cancer and normal endometrial tissues from the same patients were analyzed by cDNA microarray technology (including 588 genes). General RNA was extracted from these tissues and probes were made to be hybridized with cDNA array membrane. The CD147 expressions in normal endometrial tissue and in endometrial cancer tissue were identified by immunohistochemistry.


RESULTS
The endometrial cancer associated genes - IRP, Wnt-5A, 5T4 oncofetal antigen precursor, aggrecan core protein precursor, CD147, TIMP-2, were expressed highly.


CONCLUSIONS
cDNA microarray technology can simultaneously monitor the different expressions of genes from two different tissues. CD147 might be useful in assessing the progression and infiltration of endometrial cancer.
Meng
Y
Y
Department of Gynecology and Obsterics, People' Hospital, Peking University, Beijing 100044, China.
Wei
L
L
Wang
J
J
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D051926:Basigin; D014408:Biomarkers, Tumor / Q000032:analysis*; D001798:Blood Proteins; D018450:Disease Progression; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; C497886:BSG protein, human; D014408:Biomarkers, Tumor; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C079432:Bsg protein, rat; D008562:Membrane Glycoproteins; D051926:Basigin