Monocyte cell adhesion induced by a human aminoacyl-tRNA synthetase-associated factor, p43: identification of the related adhesion molecules and signal pathways.
An aminoacyl-tRNA synthetase-associated factor, p43, was recently shown to be secreted to induce a proinflammatory response. Because a proinflammatory response involves the cell-cell adhesion between endothelial and immune cells, we first examined the mechanism of p43-induced cell-cell adhesion of myelomonocytic leukemia cells. Intercellular adhesion molecule-1 (ICAM-1) was up-regulated by p43 and mediated p43-induced cell-cell adhesion via the interaction with LFA-1 or Mac-1. We also investigated p43-stimulated signaling pathways involved in the homotypic THP-1 cell adhesion. Because the specific inhibitors for PI3-K (phosphatidylinositol 3-kinase), ERK (extracellular signal-regulating kinase), and p38 MAPK (mitogen-activated protein kinase) blocked p43-stimulated ICAM-1 expression and homotypic THP-1 cell adhesion, these kinases were responsible for p43-induced cell-cell adhesion. p43-Dependent activation of ERK was inhibited by PI3-K inhibitors, and the activation of p38 MAPK was not. Thus, the results of this work suggest that p43 should induce cell-cell adhesion via the PI3-K/ERK- and p38 MAPK-dependent up-regulation of ICAM-1.
Park
Heonyong
H
National Creative Research Initiatives Center for ARS Network, College of Pharmacy, Seoul National University, Korea.
Park
Sang Gyu
SG
Lee
Joong-Won
JW
Kim
Taeho
T
Kim
Gyuyoup
G
Ko
Young-Gyu
YG
Kim
Sunghoon
S
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology; D009000:Monocytes / Q000166:cytology / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D017384:Sequence Deletion; D015398:Signal Transduction / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases
D018799:Intercellular Adhesion Molecule-1; D019869:Phosphatidylinositol 3-Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000604:Amino Acyl-tRNA Synthetases