Pulmonary surfactant and inflammation in septic adult mice: role of surfactant protein A.
Surfactant alterations, alveolar cytokine changes, and the role of surfactant protein (SP)-A in septic mice were investigated. Sepsis was induced via cecal ligation and perforation (CLP). Septic and sham mice were euthanized at 0, 3, 6, 9, 12, 15, and 18 h after surgery. Mice deficient in SP-A and mice that overexpressed SP-A were euthanized 18 h after surgery. In wild-type, sham-operated mice, surfactant pool sizes were similar at all time points, whereas in the CLP groups there was a significant decrease in small-aggregate surfactant pool sizes beginning 6 h after CLP. Interleukin-6 concentrations in bronchoalveolar lavage fluid from septic animals increased from 6 to 18 h after surgery. Identical surfactant alterations and concentrations of cytokines were observed in septic mice that were SP-A deficient or that overexpressed SP-A. In conclusion, alterations of pulmonary surfactant and alveolar cytokines occur simultaneously, 6 h after a systemic insult. In addition, we did not detect a role for SP-A in regulating surfactant phospholipid pool sizes or pulmonary inflammation in septic mice.
Malloy
Jaret L
JL
Department of Physiology, Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada N6A 4V2.
Veldhuizen
Ruud A W
RA
McCormack
Francis X
FX
Korfhagen
Thomas R
TR
Whitsett
Jeffery A
JA
Lewis
James F
JF
D000818:Animals; D001424:Bacterial Infections / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D009994:Osmolar Concentration; D011014:Pneumonia / Q000382:microbiology* / Q000503:physiopathology*; D011510:Proteolipids / Q000235:genetics / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000378:metabolism; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D012016:Reference Values; D013997:Time Factors
D016207:Cytokines; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants