Strain preference in donor and recipient for production of W-bearing sperm in mixed-sex germline chimeric chickens.
To elucidate the strain preference in donor and recipient for the production of W-bearing sperm, mixed-sex germline chimeric chickens were produced. The combination of donor and recipient was White Leghorn (WL) and Barred Plymouth Rock (BPR), and vice versa. Four sets of mixed-sex chimeras that had the male phenotype at sexual maturity were subjected to analysis: group 1, a female WL donor and a male BPR recipient; group 2, a male WL donor and a female BPR recipient; group 3, a female BPR donor and a male WL recipient; group 4, a male BPR donor and a female WL recipient. The mean number of W-bearing sperm detected by in situ hybridization among 10000 sperm observed was 135, 158, 26 and 71 in groups 1, 2, 3 and 4, respectively. The number in group 1 was significantly higher than that of group 3 (P<0.05). And the number in group 2 was significantly higher than those of groups 3 and 4 (P<0.05). It is suggested that the combination of a WL donor and a BPR recipient produced W-bearing sperm more efficiently than the reverse combination.
Kagami
Hiroshi
H
Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano 399-4598, Japan. kagami@gipmc.shinshu-u.ac.jp
Iwata
Junko
J
Yasuda
Jun
J
Ono
Tamao
T
D000818:Animals; D017849:Animals, Inbred Strains; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens / Q000145:classification* / Q000235:genetics*; D002678:Chimera / Q000196:embryology / Q000235:genetics*; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012727:Sex Characteristics; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
D012333:RNA, Messenger