Fine-mapping of the B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen.
In this study, we report the exact localization and substitutional characterization of a B-cell epitope domain at the N-terminus of the preS2 region of the hepatitis B surface antigen. A set of deletion variants containing preS2 sequences of different length was generated on the basis of frCP as a carrier. It was found after Western blot analysis that three monoclonal antibodies (MAbs) (2-11B1, 3-11C2, HB.OT10) recognized the linear preS2 sequence within the amino acid (aa) stretch 3-WNSTTFHQTLQDP-13. The importance of each aa residue of the epitope was proved by comparison of antibody binding to alanine-substituted peptides in both free-peptide and Pepscan variants.
Sominskaya
Irina
I
Biomedical Research and Study Centre, University of Latvia, Ratsupites Str. 1, LV-1067, Riga, Latvia. irina@biomed.lu.lv
Paulij
Wilma
W
Jansons
Juris
J
Sobotta
Dirk
D
Dreilina
Dzidra
D
Sunnen
Cecile
C
Meisel
Helga
H
Gerlich
Wolfram H
WH
Pumpens
Paul
P
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014759:Viral Envelope Proteins; C050807:presurface protein 2, hepatitis B surface antigen