Stress response to waterborne Cu during early life stages of carp, Cyprinus carpio.
Following in vitro fertilization, eggs/embryos and larvae of the common carp (Cyprinus carpio) were exposed to 0 (control), 0.3 or 0.8 micromol.l(-1) Cu in artificially prepared fresh water for 168 h. The total amounts of Cu, Na, Ca, adrenocorticotropic hormone (ACTH), alpha-melanocyte-stimulating hormone (alpha-MSH) and cortisol were measured in homogenates of eggs (up to 60 h post fertilization, hpf), isolated embryos (between 60 hpf and hatching) and free-swimming larvae. Only in embryos of eggs exposed to 0.8 micromol.l(-1) Cu a significant accumulation of Cu was observed as well as a concurrent increase in the incidence of spinal cord deformation and larval mortality. Further, when exposed to 0.8 micromol.l(-1) Cu, the whole-body Ca and Na contents were lower at 48 and 72 hpf compared to the controls and those exposed to 0.3 micromol.l(-1) Cu. Conversely, in larvae (>72 hpf) exposed to 0.3 micromol.l(-1) Cu, the Ca content was elevated from 96 hpf onwards. At 48 hpf and onwards, the whole-body ACTH and cortisol contents of the embryos exposed to 0.8 micromol.l(-1) Cu were higher than those in either controls or those exposed to 0.3 micromol.l(-1) Cu. By 96 hpf, ACTH and cortisol contents of the group exposed to 0.3 micromol.l(-1) Cu also surpassed those in controls. The alpha-MSH content in both Cu exposed groups was lower than in controls from 48 hpf onwards. It thus appears that ACTH cells and MSH cells in early life stages of carp exposed to waterborn Cu respond differently; we conclude that in prehatch carp pituitary corticotropes and interrenal cortisol producing cells respond to the chemical stressor Cu and that the resulting hormonal changes provide a sensitive diagnosis for stress as well as toxicity tests.
Flik
Gert
G
Department of Animal Physiology, Faculty of Science, University of Nijmegen, Toernooiveld 1, 6525, ED Nijmegen, The Netherlands. gertflik@sci.kun.nl
Stouthart
Xander J H X
XJ
Spanings
F A Tom
FA
Lock
Robert A C
RA
Fenwick
James C
JC
Wendelaar Bonga
Sjoerd E
SE
D000324:Adrenocorticotropic Hormone / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000196:embryology / Q000502:physiology*; D003300:Copper / Q000633:toxicity*; D004781:Environmental Exposure; D005307:Fertilization in Vitro / Q000662:veterinary; D006854:Hydrocortisone / Q000032:analysis* / Q000378:metabolism; D007814:Larva; D013116:Spinal Cord / Q000002:abnormalities; D013312:Stress, Physiological / Q000662:veterinary; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000521:alpha-MSH / Q000032:analysis* / Q000378:metabolism
D014874:Water Pollutants, Chemical; D000521:alpha-MSH; D003300:Copper; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone