[Study on the FcepsilonRI-beta polymorphism and susceptibility of asthma in a Chinese population].
OBJECTIVE
To investigate the linkage between the polymorphisms of FcepsilonRI-beta gene and susceptibility of asthma in a Chinese population.


METHODS
Thirty-two families with 192 samples and 122 cases of childhood asthma were studied. A coding variant of E237G was detected by PCR/XmnI restriction endonuclease digestion. Two RsaI polymorphic sites within non-coding region were tested by PCR/RsaI restriction endonuclease digestion.


RESULTS
The allele frequencies for all three polymorphisms of FcepsilonRI-beta gene in the Chinese population were markedly different from those published results of Caucasians and Japanese. Comparing asthma family samples with normal control, we found that genotypes containing A allele within intron 2 polymorphic site were associated with asthma (P < 0.05, OR = 2.039). The level of total serum IgE showed significant difference among three genotypes of intron 2 polymorphic site (P < 0.05). The association between A allele and elevated total serum IgE was also significant (P < 0.05, RR = 1.361). We could not find any significant results from the other two polymorphisms, even in the coding variant E237G.


CONCLUSIONS
We were unable to confirm that the FcepsilonRI-beta gene was crucial to the susceptibility of asthma and atopy and did not contribute substantially to variation in IgE levels from both sample groups.
Chen
H
H
Department of Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical sciences, Beijing 100005, China.
Chen
Y
Y
Hu
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D001249:Asthma / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D007438:Introns / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D017354:Point Mutation; D011110:Polymorphism, Genetic; D017455:Receptors, IgE / Q000235:genetics*
D017455:Receptors, IgE