Involvement of adenine nucleotide carrier in the mechanism of the apolipoprotein C induced decrease of membrane potential in rat liver mitochondria.
Apolipoprotein C (apo C) was shown to decrease the Ca2+ capacity and membrane potential of mitochondria isolated from rat liver. The specific ligands of adenine nucleotide carrier, ADP and carboxyatractyloside (CAT), inhibited the effect of apo C on the mitochondrial membrane potential. The effect of ADP and CAT was revealed in the absence of Ca2+. We conclude that in the presence of apo C, adenine nucleosides carrier transforms into a pore, and this causes the decrease in the membrane potential of the mitochondria. ADP and CAT support the primary conformation of the carrier and therefore inhibit the effect of apolipoprotein C.
Panin
L E
LE
Research Institute of Biochemistry, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk. lmmmv@cyber.ma.nsc.ru
Shalbueva
N I
NI
Kolpakov
A R
AR
Polyakov
L M
LM
D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001056:Apolipoproteins C / Q000502:physiology*; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000502:physiology*; D000226:Mitochondrial ADP, ATP Translocases / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
D001056:Apolipoproteins C; D001278:Atractyloside; D000244:Adenosine Diphosphate; D000226:Mitochondrial ADP, ATP Translocases; C003853:carboxyatractyloside; D002118:Calcium