[IL-8 and IL-10 levels in endometriosis and regulated role of neiyifang on them].
OBJECTIVE
To investigate the IL-8 and IL-10 levels in endometriosis and the regulated role of Neiyifang (NYF) on them.


METHODS
Animal models with endometriosis after Cummings method was established. IL-8 and IL-10 levels were determined in using double antibody sandwich ELISA.


RESULTS
The IL-8 levels of serum and peritoneal fluid, and peritoneal macrophage amounts were markedly higher in the untreated group than those in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were markedly lower in the treated group of NYF, compared with the untreated group. The lipopolysaccharide stimulated IL-10 secretion in peritoneal macrophages from the untreated group were significantly lower compared with the control group and the treated group of NYF.


CONCLUSIONS
The abnormality of IL-8 and IL-10 levels in endometriosis may be related to the pathogenesis of the disease. The therapeutical mechanism of NYF was mediated by promotion of IL-10 secretion, and further restrained the composition of inflammatory media and the growth of endometriotic tissue.
Yu
C
C
Changhai Hospital, Second Military Medical University, Shanghai (200433).
Liu
Y
Y
Peng
X
X
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D016209:Interleukin-8; D016753:Interleukin-10