Human osteosarcoma-derived cell lines produce soluble factor(s) that induces differentiation of blood monocytes to osteoclast-like cells.
When monocytes were cocultured with human osteosarcoma-derived cells (HOS cells), multinucleated giant cell formation of monocytes was induced. Intriguingly, even when a filter was interposed between monocytes and HOS cells, polykaryocytes also appeared. The multinucleated giant cells have characters similar to osteoclast-like cells. These findings indicate that soluble factor(s) secreted from HOS cells play an important role in polykaryocyte formation from monocytes. Twelve cloned cells were established from HSOS-1 cells and their capacities of inducing osteoclasts were investigated. Three cloned cells inducing nos. 4 and 9 had an ability of inducing osteoclasts (multinucleated giant cells, TRAP, calcitonin receptor and c-src mRNAs, osteoresorbing activity), and three cells, including nos. 1 and 5, did not show the ability. HOS cells and the cloned cells expressed several cytokine mRNAs. M-CSF was detected in the culture fluids of HOS cells, which also expressed RANK and RANK/ODF/OPGL mRNAs. Intriguingly, HOS cells secreting a soluble osteoclast inducing factors(s) expressed TNF-alpha converting enzyme mRNA. Furthermore, OCIF/OPG inhibited HOS cell-induced osteoclastogenesis and soluble RANKL could be detected in the culture fluids of HOS cells expressing TACE, suggesting that one of soluble osteoclast-inducing factor(s) is soluble RANKL. When blood monocytes were indirectly cocultured with HSOS-1 cells or cloned no. 9 cells in the presence of OCIF for 14 days, HOS cell-mediated osteoclastogenesis was suppressed, indicating that RANK-RANKL system is involved in the HOS cell-mediated osteoclastogenesis.
Miyamoto
Noriki
N
Department of Microbiology, Mie University School of Medicine, Tsu, Japan.
Higuchi
Yasumitsu
Y
Mori
Kouki
K
Ito
Morihiro
M
Tsurudome
Masato
M
Nishio
Machiko
M
Yamada
Hiroyuki
H
Sudo
Akihiro
A
Kato
Ko
K
Uchida
Atsumasa
A
Ito
Yasuhiko
Y
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors; D000293:Adolescent; D000818:Animals; D001685:Biological Factors / Q000096:biosynthesis* / Q000502:physiology; D001859:Bone Neoplasms / Q000378:metabolism*; D001862:Bone Resorption / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D002459:Cell Fusion; D002460:Cell Line; D002648:Child; D002999:Clone Cells; D018920:Coculture Techniques; D004396:Coloring Agents; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D051379:Mice; D009000:Monocytes / Q000502:physiology*; D010010:Osteoclasts / Q000502:physiology*; D012516:Osteosarcoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase; D014407:Tumor Cells, Cultured
D001685:Biological Factors; D004396:Coloring Agents; D018076:DNA, Complementary; D007527:Isoenzymes; D012333:RNA, Messenger; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase