Differentiation of Herpes simplex virus types 1 and 2 in sera of patients with HSV central nervous system infections by type-specific enzyme-linked immunosorbent assay.
OBJECTIVE
To differentiate by enzyme-linked immunosorbent assay (ELISA) using type-specific glycoprotein G herpes simplex virus (HSV) type 1 and type 2 in serum collected from patients with HSV central nervous system (CNS)infections.


METHODS
HSV 1 and 2 typing in convalescent sera of 17 patients with HSV acute encephalitis, myelitis, or meningitis was determined by the type-specific IgG ELISA kit (Gull Laboratory, Inc.). HSV CNS infections were diagnosed by polymerase chain reaction (PCR) or conventional serologic tests from acute to convalescent stages.


RESULTS
In 13 of 17 patients, HSV type 1 and HSV type 2 antibodies were positive; 11 patients with HSV type 1 and 2 patients with HSV type 2 were found. All 10 patients with encephalitis showed equivocal or positive results for HSVtype 1. In two of three cases of myelitis, HSV type 1 was demonstrated. Each case of myelitis and meningitis reacted to both types 1 and 2.


CONCLUSIONS
These data suggest that the kit is useful for type differentiation of HSV CNS infections from convalescent sera, and can play a supplementary role in HSV typing by PCR or previous serologic tests.
Nishimura
Y
Y
First Department of Internal Medicine, Kurume University School of Medicine, Kurume, Japan.
Ayabe
M
M
Shoji
H
H
Hashiguchi
H
H
Eizuru
Y
Y
Kawana
T
T
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood* / Q000134:cerebrospinal fluid; D002494:Central Nervous System Infections / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012698:Serologic Tests; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C045464:glycoprotein G, herpes simplex virus type 2; C038535:glycoprotein gC, herpes simplex virus type 1