Cyclo-oxygenase- and capsaicin-sensitive afferent fibres affect Beta-adrenoceptor-evoked response in the rat urinary bladder.
As reported previously, the beta-adrenoceptor-evoked response in the in vitro rabbit detrusor is inhibited by non-steroidal anti-inflammatory drugs (NSAIDs). Prostaglandins can restore this response. This study was designed to better define physiological influences on beta-adrenoceptors in the urinary bladder and to ascertain whether the observations made in the rabbit urinary bladder in vitro were valid in vivo, as well as in a different animal species, i.e. in the rat. The NSAID indometacin partially inhibited the isoproterenol-evoked response in the rat detrusor muscle. When the neurokinin A (NKA) antagonist MEN-10376 was administered after indometacin, the beta-adrenoceptor-evoked response was completely abolished. The isoproterenol-evoked beta response was dose dependently reduced following treatment with the capsaicin antagonist ruthenium red. Taken together, these data indicate an involvement of prostanoids, NKA- and capsaicin-sensitive fibres in the beta-adrenoceptor-evoked response in the rat urinary bladder.
Tucci
Paolo
P
Dipartimento di Farmacologia delle Sostanze Naturali e Fisiologia Generale, Università degli Studi di Roma I (La Sapienza), Pie Aldo Moro, I-00185 Rome, Italy. Paolo.tucci@uniroma1.it
Bartocci
C
C
Bolle
P
P
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002211:Capsaicin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009130:Muscle, Smooth / Q000502:physiology; D015288:Neurokinin A / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000502:physiology; D012430:Ruthenium Red / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology; D017833:Visceral Afferents / Q000187:drug effects / Q000502:physiology
D000318:Adrenergic beta-Agonists; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010446:Peptide Fragments; D011453:Prostaglandins; D011943:Receptors, Adrenergic, beta; D012430:Ruthenium Red; C069192:neurokinin A(4-10), Tyr(5)-Trp(6,8,9)-Lys(10)-; D015288:Neurokinin A; D010208:Papaverine; D011451:Prostaglandin-Endoperoxide Synthases; D007545:Isoproterenol; D002211:Capsaicin; D007213:Indomethacin