Cloning and identification of an angiostatic molecule IP10/crg -2.
AIM:To obtain human and murine cDNAs encoding IFN-gamma inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2).METHODS:The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-gamma and TNF-alpha,respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+).RESULTS:The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing.CONCLUSION:Recombinant IP-10/crg-2 gene clones with 306bp and 314bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2.
Liu
Zhi-Guo
ZG
Yang
Jing-Hua
JH
An
Hua-Zhang
HZ
Wang
Hai-Yan
HY
He
Feng-Tian
FT
Han
Zhe-Yi
ZY
Han
Ying
Y
Wu
Han-Ping
HP
Xiao
Bing
B
Fan
Dai-Ming
DM