[Assembly and evaluation of tissue engineered human cellular alveolar bony implanting materials in vitro].
OBJECTIVE
To assembly human cellular alveolar bony implanting materials in vitro and evaluate their osteogenic activities.


METHODS
Human alveolar bone cells were separated from alveolar bone around the third impacted teeth of 3 patients by enzyme digestion and cultured in alpha-MEM containing beta-glycerophosphate and Dexamethasone at 5% CO2, 37 degrees C for 21-28 days. Confirmed osteoblasts-like cells were then seeded onto 3 kinds of degradable biomaterials of polyglycolic acid scaffold, collagen sponge, and L-lactic acid/epsilon-caprolactone to form the cell-scaffold complexes. The 3 types of complexes were continued to culture for 21-28 days at the same conditions. The cell proliferation, morphological changes, ALPase activity and mineral nodule formation on scaffolds were measured and observed at 3 days intervals.


RESULTS
The results indicated that the cultured human alveolar bone origin cells from 3 patients could successfully express the osteoblasts phenotype in single layered culturing after stimulated by beta-glycerophosphate and Dexamethasone. The cultured osteoblast-like cells seeded on PGAS matrix had the highest attachment, proliferative and osteogenic activities, suggesting a good bio-affinity between the human alveolar osteoblast-like cells and the PGAS matrix. The statistical analysis (ANOVA) showed that there were significant differences between PGAS- osteoblasts complex and CLGS or LACT complexes on osteogenic activities (P < 0.05).


CONCLUSIONS
PGAS-osteoblast complex is worth to be further developed into a tissue-engineered cellular artificial bony implant for reconstructing the oral-maxillofacial bony defects.
Yao
H
H
Department of Biomedical Engineering, Tianjin Stomatological Hospital, Tianjin, 300041 China.
D020341:Absorbable Implants; D000539:Alveolar Process / Q000166:cytology*; D002478:Cells, Cultured; D006801:Humans; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis; D023822:Tissue Engineering