title: High frequencies of HGV and TTV infections in blood donors in Hangzhou.
abstract: OBJECTIVE
  To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou.
  
  
  METHODS
  RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.
  
  
  RESULTS
  Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively.
  
  
  CONCLUSIONS
  The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.
authors.affiliation: Department of Pathogenic Biology, Medical School of Zhejiang University, 353 Yan An Road, Hangzhou 310006, Zhejiang Province,China. yanchen@mail.hz.zj.cn
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.forename: L L
authors.initials: LL
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Luo
authors.forename: Y F
authors.initials: YF
authors.lastname: Mao
authors.forename: M
authors.initials: M
authors.lastname: He
mesh_terms: D001483:Base Sequence; D001782:Blood Donors / Q000706:statistics & numerical data*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis / Q000097:blood; D029407:GB virus C / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000175:diagnosis / Q000453:epidemiology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000097:blood; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D065227:Transfusion Reaction
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral