MMP-7 (matrilysin) accelerated growth of human umbilical vein endothelial cells.
Matrix metalloproteinases (MMP) are considered to play important roles in angiogenesis. In angiogenic processes, endothelial cells secrete MMP-2 or MMP-1 to dissolve the basement membrane or connective tissue around the vessels. MMP-7 (matrilysin) is secreted from the neovasculars induced by cancer and is a metastatic factor of colorectal cancer. The effect of matrilysin on angiogenesis is still unclear, however. We therefore examined the effect of MMP-7 on the proliferation of human umbilical vein endothelial cells (HUVECs) in vitro. Our results showed that recombinant MMP-7 (rMMP-7) accelerated the proliferation of endothelial cells dose-dependently, and did so for endothelial cells cultured not only on type IV collagen, but also on type I collagen. MMP-7 also upregulated MMP-1, -2 secretion, but did not stimulate vascular endothelial growth factor (VEGF) secretion. From this study, we conclude that MMP-7 directly induces angiogenesis, and that therefore MMP-7 would be a good target of cancer therapy.
Huo
Nailin
N
Department of Surgery-2, Yokohama City University School of Medicine, 3-9 Fukuura kanazawa-ku, Yokohama 236-0004, Japan.
Ichikawa
Yasushi
Y
Kamiyama
Masako
M
Ishikawa
Takashi
T
Hamaguchi
Yohei
Y
Hasegawa
Satoshi
S
Nagashima
Yoji
Y
Miyazaki
Kaoru
K
Shimada
Hiroshi
H
D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000378:metabolism; D016228:Endothelial Growth Factors / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005780:Gelatin / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D008222:Lymphokines / Q000378:metabolism; D020783:Matrix Metalloproteinase 7 / Q000494:pharmacology*; D011994:Recombinant Proteins; D014471:Umbilical Veins / Q000166:cytology; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D017077:Culture Media, Conditioned; D016228:Endothelial Growth Factors; D008222:Lymphokines; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D005780:Gelatin; D003094:Collagen; D020783:Matrix Metalloproteinase 7