Application of Candida solubilized cell wall beta-glucan in antitumor immunotherapy against P815 mastocytoma in mice.
This study was designed to evaluate the antitumor activity of CSBG, purified from the cell wall of Candida albicans IFO1385. First, as an effect of CSBG on P815 mastocytoma, significant prolonged survival and suppression of the tumor growth were observed. Second, the transfer of spleen cells from CSBG-sensitized BALB/c mice to CDF1 mice led to further suppression of tumor growth as well as P815-immunized spleen cells. Third, CSBG enhanced antitumor immunity in gene therapy using B7-1-transfected P815 cells. These results strongly suggest that CSBG enhances the host defense response to tumor due in part to an adjuvant effect.
Tokunaka
Kazuhiro
K
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Ohno
Naohito
N
Adachi
Yoshiyuki
Y
Miura
Noriko N
NN
Yadomae
Toshiro
T
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics; D002175:Candida / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry*; D003131:Combined Modality Therapy; D005260:Female; D005434:Flow Cytometry; D015316:Genetic Therapy; D005936:Glucans / Q000737:chemistry / Q000627:therapeutic use*; D016219:Immunotherapy, Adoptive; D007202:Indicators and Reagents; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D012515:Mast-Cell Sarcoma / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D010957:Plasmids / Q000235:genetics; D016019:Survival Analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
D018122:B7-1 Antigen; D005936:Glucans; D007202:Indicators and Reagents