Determination of a non-methylated deoxycytidine residue in the recognition site of DNA-methyltransferases.
A method for determination of a non-methylated deoxycytidine (dC) residue in the recognition site of 5-cytosine DNA-methyltransferases is suggested. The method is based on treatment of methylated DNA by sodium bisulfite and successive reaction of the thus modified DNA with a repair enzyme, uracil-DNA glycosylase. This method was successfully applied to identify NlaX methyltransferase specificity.
Kubareva
E A
EA
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119899 Russia. kubareva@genebee.msu.su
Walter
J
J
Vorob'eva
O V
OV
Razumikhin
M V
MV
Karyagina
A S
AS
Lau
P C
PC
Trautner
T
T
D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D045647:DNA Glycosylases; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000378:metabolism*; D003841:Deoxycytidine / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009343:Neisseria / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D013447:Sulfites / Q000737:chemistry; D051981:Uracil-DNA Glycosidase
D011994:Recombinant Proteins; D013447:Sulfites; D003841:Deoxycytidine; D004247:DNA; D015254:DNA Modification Methylases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; C009279:sodium bisulfite