title: SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro.
abstract: SEL1L, the human orthologue of the Caenorhabditis elegans sel-1 gene, is differentially expressed in breast primary tumors and in normal breast tissues. Analysis of a series of human primary breast carcinomas, using a monoclonal antibody raised against a SEL1L recombinant protein, revealed down-modulation or absence of SEL1L expression in about two-thirds of the tumors as compared with normal breast epithelial cells. Overall survival analysis of breast carcinoma patients indicated a statistically significant correlation between SEL1L down-modulation and poor prognosis. MCF-7, human breast carcinoma cells, were transfected with a construct containing the entire SEL1L cDNA driven by an inducible promoter and showed a dramatic reduction in anchorage-dependent growth and colony formation in soft agar. Growth of the transfected cells in Matrigel, an extracellular matrix rich with laminin, restored colony-formation ability. These results point to the role for SEL1L in breast tumor growth and aggressiveness, possibly involving cell-matrix interactions.
authors.affiliation: Molecular Targeting Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, 20133 Milan, Italy.
authors.forename: Rosaria
authors.initials: R
authors.lastname: Orlandi
authors.forename: Monica
authors.initials: M
authors.lastname: Cattaneo
authors.forename: Flavia
authors.initials: F
authors.lastname: Troglio
authors.forename: Patrizia
authors.initials: P
authors.lastname: Casalini
authors.forename: Chiara
authors.initials: C
authors.lastname: Ronchini
authors.forename: Sylvie
authors.initials: S
authors.lastname: Ménard
authors.forename: Ida
authors.initials: I
authors.lastname: Biunno
mesh_terms: D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018275:Carcinoma, Lobular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016133:Polymerase Chain Reaction; D011379:Prognosis; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D011506:Proteins; C117384:SEL1L protein, human; D003907:Dexamethasone