Demonstration of placental vascular anatomy in monochorionic twin gestations.
Invasive treatment modalities for severe chronic twin-to-twin transfusion syndrome (TTTS), such as fetoscopic laser coagulation of communicating vessels, have revived the need for detailed studies of placental angioarchitecture. We describe a practical placental vascular injection technique using alcohol-resistant tissue-staining dyes. Injection of color-coded gelatin-dye mixtures effectively delineated the intertwin vasculature, and allowed unequivocal macroscopic classification of vascular communications as artery-to-artery, vein-to-vein, or deep artery-to-vein anastomoses. The existence of deep artery-to-vein anastomoses was further confirmed by light microscopic demonstration of venous dye of one twin and arterial dye of the opposite twin within the same stem villus. Furthermore, the injection technique allowed determination of the caliber of the anastomoses, the direction of the artery-to-vein anastomoses, and the relative vascular territory of each twin. Documenting the vascular communications in monochorionic twin placentas with and without TTTS may enhance our understanding of the pathogenesis of chronic TTTS. Correlating the anastomotic patterns and location of the laser coagulation scars with post-ablation outcome will aid in the design of rational therapeutic methods for this often lethal condition.
De Paepe
Monique E
ME
Department of Pathology, Women and Infants' Hospital, 101 Dudley Street, Providence, RI 02905, USA.
Burke
Sarah
S
Luks
Francois I
FI
Pinar
Halit
H
Singer
Don B
DB
D000328:Adult; D001163:Arteriovenous Anastomosis / Q000473:pathology; D002823:Chorion / Q000098:blood supply; D004200:Diseases in Twins; D005260:Female; D005330:Fetofetal Transfusion / Q000209:etiology / Q000473:pathology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D010920:Placenta / Q000098:blood supply*; D021041:Placental Circulation / Q000502:physiology*; D011247:Pregnancy; D014429:Twins, Dizygotic