Purification and preliminary crystallographic study of Trametes versicolor laccase in its native form.
Laccases are multi-copper oxidases that catalyse the oxidation of a wide range of phenols and their use in industrial oxidative processes is increasing. A laccase has been purified from the fungus Trametes versicolor and crystallized using the hanging-drop method. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 87.7, b = 110.5, c = 123.2 A, beta = 103.4 degrees. A complete data set was collected to 2.4 A resolution on a Cu K(alpha) rotating-anode X-ray source. Molecular replacement was performed and the initial electron-density maps indicate that the four expected Cu atoms are all present.
Bertrand
Thomas
T
Laboratoire d'Enzymologie et de Biochimie Structurales, UPR 9063 du CNRS, 91198 Gif-sur-Yvette CEDEX, France.
Jolivalt
Claude
C
Caminade
Eliane
E
Joly
Nathalie
N
Mougin
Christian
C
Briozzo
Pierre
P
D001487:Basidiomycota / Q000201:enzymology*; D003300:Copper / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D042845:Laccase; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D011487:Protein Conformation
D003300:Copper; D010088:Oxidoreductases; D042845:Laccase