Antimutagenic effects of garlic extract on chromosomal aberrations.
Garlic (Allium sativum) has been used since ancient times, as a spice and also for its medicinal properties. In present set of investigations antimutagenic effect of garlic extract (GE) has been evaluated using 'in vivo chromosomal aberration assay' in Swiss albino mice. Cyclophosphamide (CP), a well-known mutagen, was given at a single dose of 25 mg/kg b.w. intraperitoneally. Pretreatment with 1, 2.5 and 5% of freshly prepared GE was given through oral intubation for 5 days prior to CP administration. Animals from all the groups were sacrificed at sampling times of 24 and 48 h and their bone marrow tissue was analyzed for chromosomal damage. The animals of the positive control group (CP alone) shows a significant increase in chromosomal aberrations both at 24 and 48 h sampling time. GE, alone did not significantly induced aberrations at either sampling time, confirming its non-mutagenicity. However in the GE pre-treated and CP post-treated groups, a dose dependent decrease in cytogenetic damage was recorded. A significant suppression in the chromosomal aberrations was recorded following pretreatment with 2.5 and 5% GE administration. The anticytotoxic effects of GE were also evident, as observed by significant increase in mitotic index, when compared to positive control group. Reduction in CP induced clastogenicity by GE was evident at 24 h and to a much greater extent at 48 h of cell cycle. Thus results of the present investigations revealed that GE has chemopreventive potential against CP induced chromosomal mutations in Swiss albino mice.
Shukla
Yogeshwer
Y
Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, P.O. Box 80, MG Marg, Lucknow 226 001, India. yogeshwer_shukla@hotmail.com
Taneja
Pankaj
P
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations; D003520:Cyclophosphamide; D004305:Dose-Response Relationship, Drug; D005737:Garlic; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D010936:Plant Extracts / Q000627:therapeutic use*; D013997:Time Factors
D009153:Mutagens; D010936:Plant Extracts; D003520:Cyclophosphamide