Novel functional biodegradable polymer: synthesis and anticoagulant activity of poly(gamma-glutamic acid)sulfonate (gamma-PGA-sulfonate).
gamma-Poly(glutamic acid) (gamma-PGA), which is produced by Bacillus subtilis, was sulfonated using 2-aminoethane-1-sulfonic acid (taurine) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (WSC) to give sulfonated gamma-PGA (gamma-PGA-sulfonate). From (1)H NMR spectroscopy and IR spectroscopy, it was confirmed that taurine was introduced to the side chain of gamma-PGA via an amide linkage. By altering the synthetic conditions, it was possible to control the content of sulfonate in gamma-PGA-sulfonate. Anticoagulant activity was investigated in order to evaluate the biological activity of gamma-PGA-sulfonate by the Lee-White test. The clotting time was prolonged when the concentration of gamma-PGA-sulfonate on the degree of sulfonation was increased. It becomes clear that gamma-PGA-sulfonate is potentially useful for various medical applications, such as drug delivery, tissue engineering, and medical materials.
Matsusaki
Michiya
M
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, I-21-40 Korimoto, Kagoshima 890-0065, Japan.
Serizawa
Takeshi
T
Kishida
Akio
A
Endo
Takeshi
T
Akashi
Mitsuru
M
D000925:Anticoagulants / Q000138:chemical synthesis* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D008970:Molecular Weight; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology*; D012995:Solubility; D012996:Solutions; D013654:Taurine / Q000737:chemistry
D000925:Anticoagulants; D004952:Esters; D012996:Solutions; C451199:poly(glutamic acid)sulfonate; D013654:Taurine; D011099:Polyglutamic Acid