title: Antinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals.
abstract: In the present study, we have attempted to elucidate the active components for rheumatoidal arthritis using chloroform (CHCl(3)), ethylacetate (EtOAc) and n-butanol (BuOH) fractions of the methanol extract (MeOH) of Kalopanax pictus. Kalopanaxsaponin-A and -I (KPS-A and -I, hederagenin monodesmoside) were isolated from EtOAc fraction and kalopanaxsaponin-B, -H and -K (KPS-B, -H and -K, hederagenin bisdesmosides) obtained from BuOH fraction, respectively. MeOH extract, EtOAc fraction (250, 500 mg/kg, p.o.) and KPS-A and -I (5, 10, 20 mg/kg, i.p.) exhibited significant antinociceptive effects, which were determined by acetic acid-induced writhing test and hot plate test. On Freund's complete adjuvant reagent-induced rheumatoidal arthritis in rats, the administration of EtOAc fraction and KPS-A and -I inhibited edema, agglutination, vascular permeability and trypsin inhibitor. In addition, LD(50) of the MeOH extract was shown to be 4.033 mg/kg. These results suggest that anti-rheumatoidal effects of KPS-A and -I contribute to the inhibition of kinin formation by suppression of trypsin inhibitor activity.
authors.affiliation: College of Pharmacy, Kyungsung University, Pusan 608-736, South Korea.
authors.forename: Jongwon
authors.initials: J
authors.lastname: Choi
authors.forename: Keun
authors.initials: K
authors.lastname: Huh
authors.forename: Suk-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Kyung-Tae
authors.initials: KT
authors.lastname: Lee
authors.forename: Hee-Juhn
authors.initials: HJ
authors.lastname: Park
authors.forename: Yong Nam
authors.initials: YN
authors.lastname: Han
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D018501:Antirheumatic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*; D027481:Araliaceae / Q000737:chemistry*; D001169:Arthritis, Experimental / Q000188:drug therapy; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010147:Pain Measurement / Q000187:drug effects / Q000706:statistics & numerical data; D008517:Phytotherapy / Q000379:methods / Q000706:statistics & numerical data; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D000700:Analgesics; D018501:Antirheumatic Agents; D010936:Plant Extracts; D012503:Saponins; C010177:beta-hederin; C112799:kalopanax saponin H; C117027:kalopanax saponin I; C064714:kalopanax saponin B; D009828:Oleanolic Acid