Role of phospholipid transfer protein in rabbit lung development.
A 36-kDa phospholipid transfer protein (PLT-P(R)), which preferentially transfers phosphatidyl choline (PC) compared to phosphatidyl inositol (PI), was purified 827-fold from rabbit lung homogenate. Incorporation of cholesterol in unilamellar vesicles reduced the PC transfer activity of PLTP(R). Dipalmitoyl phosphatidyl choline uptake by alveolar type II cells was increased in the presence of the protein, and further enhanced in the presence of surfactant liposomes. However, a decrease in uptake was noted with cholesterol in host membranes. Incorporation of PI into host membranes had a low stimulatory effect on the process. All these effects were more pronounced in adult type II cells compared to premature, term and 3-day-old pups.
Kaushal
S
S
Department of Experimental Medicine & Biotechnology, PGIMER, Chandigarh, India.
Ghosh
S
S
Sharma
N
N
Sanyal
S N
SN
Majumdar
S
S
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism / Q000493:pharmacokinetics; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D002469:Cell Separation; D002784:Cholesterol / Q000737:chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000196:embryology / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D010713:Phosphatidylcholines / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits
D002352:Carrier Proteins; D008081:Liposomes; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D011663:Pulmonary Surfactants; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol