Chromosomal effects of adeno-associated virus vector integration.
Adeno-associated virus (AAV) vectors are currently being used in several clinical gene-therapy trials (see the NIH OBA Human Gene Transfer Clinical Trials Database); however, little is known about the chromosomal effects of vector integration. Here we report that integrated vector proviruses are associated with chromosomal deletions and other rearrangements and are frequently located on chromosome 19 (although not at the wildtype AAV integration site).
Miller
Daniel G
DG
Department of Medicine, University of Washington, Seattle, Washington, 98195, USA.
Rutledge
Elizabeth A
EA
Russell
David W
DW
D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D004247:DNA / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016662:Virus Integration / Q000235:genetics
D004247:DNA