Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells.
Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 cells, no induction was observed under either set of conditions. These results strongly indicate that Bach1 and ARA70 have valuable roles at the onset of adipocyte differentiation.
Nishizuka
Makoto
M
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-Oka, Suita, Osaka 565-0871, Japan.
Tsuchiya
Tomoko
T
Nishihara
Tsutomu
T
Imagawa
Masayoshi
M
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D056918:Nuclear Receptor Coactivators; D009693:Nucleic Acid Hybridization; D015513:Oncogene Proteins; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis*
C486316:Bach1 protein, mouse; D050976:Basic-Leucine Zipper Transcription Factors; D018076:DNA, Complementary; C111382:NCOA4 protein, human; D056918:Nuclear Receptor Coactivators; D015513:Oncogene Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D012313:RNA