[Priming-phenomenon of neutrophils in patients with Flexner infection].
OBJECTIVE
To study reproducibility of priming phenomenon of neutrophils in patients with acute Flexner's dysentery and its realization manifestation.


METHODS
A chemiluminescent response of peripheral blood neutrophils was studied in patients with acute mild and moderate dysentery in the presence of luminol. Lipopolysaccharide Salmonella typhimurium at a final concentration of 20 ng/ml served as a priming substance of S-chemotype. Neutrophils were stimulated with phorbol myristate acetate in concentration 10(-6) M and isolated on Histopaque double gradient (Sigma reagents, USA).


RESULTS
In reproduction of priming-phenomenon in vitro on neutrophils of patients with acute Flexner's dysentery chemiluminescence amplitude increased 1.29-1.69-fold vs control.


CONCLUSIONS
Priming-phenomenon on neutrophils in acute dysentery is reproducible. This confirms modulating properties of lipopolysaccharides in conditions of systemic nonphysiological endotoxinemia. Priming phenomenon may be involved in both maintenance of homeostasis and pathophysiological processes.
Gorobchenko
A N
AN
Malov
V A
VA
Grachev
S V
SV
Pak
S G
SG
D000208:Acute Disease; D004405:Dysentery, Bacillary / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D012485:Salmonella typhi; D012762:Shigella dysenteriae
D008070:Lipopolysaccharides