Characterization of glycans in a lactoferrin isoform, lactoferrin-a.
The presence of glycan at Asn-281 in bovine lactoferrin-a, which has a higher molecular weight than regular lactoferrin-b, was found in our previous study. The present work was performed to clarify the structures of the glycans linked to the five N-glycosylation sites in lactoferrin-a and to compare them with those of glycans linked to lactoferrin-b. In lactoferrin-a, the glycans linked to Asn-233 and Asn-545 were of the high-mannose type, whereas those present at Asn-368 and Asn-476 were complex-type ones. These glycans possessed heterogeneous structures. A comparative study of the glycans on bovine lactoferrin-a and bovine lactoferrin-b by HPLC showed that the structures of the glycans linked to Asn-368, Asn-476, and Asn-545 were very similar, the exception being the glycan linked to Asn-233. In addition, analysis of the structure of the glycan bound to Asn-281 present only in lactoferrin-a showed it possessed the heterogeneous structure of a complex-type glycan in which the structures Man3GlcNAc2, Man3GlcNAc4, Man3GlcNAc4Fuc are suggested to be present based on HPLC retention times only.
Wei
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Z
Faculty of Applied Biological Science, Hiroshima University, Higashi-hiroshima, Japan.
Nishimura
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T
Yoshida
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D000818:Animals; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D007781:Lactoferrin / Q000032:analysis / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D020033:Protein Isoforms
D011134:Polysaccharides; D020033:Protein Isoforms; D007781:Lactoferrin