[Preparation and analysis of pure type II collagen from porcine articular cartilage].
This investigation was aimed at the preparation of pure type II collagen from porcine articular cartilage and the feasible method for producing type II collagen in bulk. After dispersal of the porcine articular cartilage, the proteoglycans were extracted by guanidinium hydrochloride and dissolved by pepsin in acid solvent. The contaminants including denatured and degraded protein and other collagen was removed via the repeated procedure of purification. For obtaining the purer type II collagen, the chromatography with sepharose H. P. Column was also used. The purity of the sample was compared with the type II collagen produced by Sigma Company. Both type II collagens were characterized by SDS-PAGE electrophoresis, amino acid analysis and maximal violet chromatography, and all of the results accorded with the standard photograph in the references. The purity of the sample was higher than that of the product of Sigma Company. This prepared collagen of type II is a product of high purity. The raw materials are the common porcine articular cartilage, which is rich in resource and low in cost. Therefore, it is suitable to produce type II collagen in batches.
Li
S
S
Guangzhou Institute of Traumatology, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220.
Ye
C
C
Zou
H
H
Peng
Y
Y
Yang
X
X
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry*; D024043:Collagen Type II / Q000032:analysis / Q000302:isolation & purification*; D013552:Swine
D000596:Amino Acids; D024043:Collagen Type II