title: Noradrenergic content and turnover rate in kidney and heart shows gender and strain differences.
abstract: The objective of this study was to compare strain and gender differences in kidney and heart norepinephrine (NE) content and turnover rate in normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR, SHR/a, and SHR/y). Our laboratory has shown that the Y chromosome has a significant effect on blood pressure in the SHR model of hypertension through the use of two new rat stains, SHR/a and SHR/y, to study the Y chromosome. SHR/a have a SHR autosomal genetic background with a WKY Y chromosome, whereas the SHR/y rats have a WKY autosomal genetic background with a SHR Y chromosome. Tissues were homogenized after alpha-methyl-DL-p-tyrosine injection and analyzed for NE. The male kidney NE content was significantly lower in the WKY compared with the SHR, SHR/y, and SHR/a. Kidney and heart NE content was significantly higher in females compared with males in all strains except the SHR/y. The WKY and SHR/y females had significantly lower kidney NE turnover rates, and the SHR and SHR/a females had significantly higher kidney NE turnover rates than strain-matched males. This study suggests both a strain and gender difference in sympathetic nervous system activity through noradrenergic neurotransmission.
authors.affiliation: Department of Biology, The University of Akron, Akron, Ohio 44325-3908, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Caplea
authors.forename: Darcie
authors.initials: D
authors.lastname: Seachrist
authors.forename: Hamid
authors.initials: H
authors.lastname: Daneshvar
authors.forename: Gail
authors.initials: G
authors.lastname: Dunphy
authors.forename: Daniel
authors.initials: D
authors.lastname: Ely
mesh_terms: D000818:Animals; D005260:Female; D006973:Hypertension / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics / Q000378:metabolism*; D011921:Rats, Inbred WKY / Q000235:genetics / Q000378:metabolism*; D012727:Sex Characteristics; D013045:Species Specificity; D014998:Y Chromosome / Q000502:physiology
chemical_list: D009638:Norepinephrine