title: Response of Sod-2 enzyme activity to selection for high voluntary wheel running.
abstract: The objective of this study was to examine the correlated response of anti-oxidant enzyme activity to selective breeding for increased voluntary wheel running in house mice. Activity of liver superoxide dismutase-2 (Sod-2), a free radical scavenger, was measured in four groups of mice. 'Active' individuals were housed in cages with attached wheels for 8 weeks beginning at weaning; 'sedentary' individuals were housed in cages with attached wheels that were prevented from rotating. Both of these treatments were applied to male and female mice from generation 14 of a replicated artificial selection experiment, which is composed of four lines selected for high wheel running and four randomly bred lines that serve as controls. In females, Sod-2 activity was significantly lower in selected vs control animals, regardless of presence/absence of a free-turning wheel. This difference suggests a trade-off between early-age voluntary wheel-running activity and Sod-2 activity. In males, Sod-2 activity was significantly affected by an interaction between selection group and activity group, with males from selected lines having lower Sod-2 activity relative to control males only in the sedentary treatment. These negative correlated responses of Sod-2 activity to selection on wheel running are discussed in the context of antagonistic pleiotropy models of aging and with respect to potential effects on lifespan.
authors.affiliation: School of Biological Sciences, Washington State University, Pullman, WA 99164, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Thomson
authors.forename: T
authors.initials: T
authors.lastname: Garland
authors.forename: J G
authors.initials: JG
authors.lastname: Swallow
authors.forename: P A
authors.initials: PA
authors.lastname: Carter
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001947:Breeding; D005260:Female; D008124:Locomotion; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D005082:Physical Exertion; D012641:Selection, Genetic; D013482:Superoxide Dismutase / Q000378:metabolism*; D000099156:Superoxide Dismutase 2
chemical_list: D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2