Neuropeptide Y-induced angiogenesis in aging.
Age-related changes in NPY-driven angiogenesis were investigated using Matrigel and aortic sprouting assays in young (2 months.) and aged (18 months.) mice. In both assays, NPY-induced vessel growth decreased significantly with age. In parallel, aged mice showed reduced expression (RT-PCR) of Y2 receptors and the NPY converting enzyme, dipeptidyl peptidase IV (DPPIV), in spleens. Aging of human microvascular endothelial cells in vitro led to a loss of their mitogenic responses to NPY accompanied by a lack of NPY receptor mRNAs. Thus, NPY-dependent angiogenesis is impaired with age, which is associated with a decreased expression of endothelial NPY receptors (Y2) and DPPIV.
Kitlinska
Joanna
J
Department of Physiology and Biophysics, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20007, USA. jbk4@georgetown.edu
Lee
Edward W
EW
Movafagh
Sharareh
S
Pons
Jennifer
J
Zukowska
Zofia
Z
D000375:Aging; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation / Q000378:metabolism; D018919:Neovascularization, Physiologic; D009478:Neuropeptide Y / Q000494:pharmacology*; D011509:Proteoglycans / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D013997:Time Factors
D004338:Drug Combinations; D007797:Laminin; D009478:Neuropeptide Y; D011509:Proteoglycans; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; C059485:matrigel; D003094:Collagen; D004247:DNA; D018819:Dipeptidyl Peptidase 4