Expression and immunolocalization of the 14-3-3 protein of Schistosoma mansoni.
The 14-3-3 protein is a key player in signal transduction processes in various species. We have previously cloned and expressed the 14-3-3 of Schistosoma mansoni. Using the purified protein we have now raised antibodies against it. A highly specific, affinity-purified antibody preparation was employed for the localization of the 14-3-3 protein in the parasite, by both immunohistochemistry and immunoelectron microscopy. The results demonstrate wide distribution of this protein. It was observed in the female excretory system, the nephridia as well as in the genital systems of both sexes, namely in the vitelline gland of female and in the testis of the male. It is also present in the parenchyma and muscle of both male and female worms. Immunoelectron microscopy demonstrated the presence of immunogold-labelled protein in the tegument, subtegument, muscle, parenchyma and in the female reproductive system, in both the cytoplasm and nucleus of vitelline cells, and oocytes. The possible role of the 14-3-3 protein in the genital organs is discussed.
Schechtman
D
D
Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
Winnen
R
R
Tarrab-Hazdai
R
R
Ram
D
D
Shinder
V
V
Grevelding
C G
CG
Kunz
W
W
Arnon
R
R
D048948:14-3-3 Proteins; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D015153:Blotting, Western; D018509:DNA, Helminth / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012550:Schistosoma mansoni / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D015398:Signal Transduction / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000378:metabolism
D048948:14-3-3 Proteins; D000909:Antibodies, Helminth; D018509:DNA, Helminth; D014446:Tyrosine 3-Monooxygenase