title: Fumarate respiration of Wolinella succinogenes: enzymology, energetics and coupling mechanism.
abstract: Wolinella succinogenes performs oxidative phosphorylation with fumarate instead of O2 as terminal electron acceptor and H2 or formate as electron donors. Fumarate reduction by these donors ('fumarate respiration') is catalyzed by an electron transport chain in the bacterial membrane, and is coupled to the generation of an electrochemical proton potential (Deltap) across the bacterial membrane. The experimental evidence concerning the electron transport and its coupling to Deltap generation is reviewed in this article. The electron transport chain consists of fumarate reductase, menaquinone (MK) and either hydrogenase or formate dehydrogenase. Measurements indicate that the Deltap is generated exclusively by MK reduction with H2 or formate; MKH2 oxidation by fumarate appears to be an electroneutral process. However, evidence derived from the crystal structure of fumarate reductase suggests an electrogenic mechanism for the latter process.
authors.affiliation: Institüt für Mikrobiologie, Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany. a.kroeger@em.uni-frankfurt.de
authors.forename: Achim
authors.initials: A
authors.lastname: Kröger
authors.forename: Simone
authors.initials: S
authors.lastname: Biel
authors.forename: Jörg
authors.initials: J
authors.lastname: Simon
authors.forename: Roland
authors.initials: R
authors.lastname: Gross
authors.forename: Gottfried
authors.initials: G
authors.lastname: Unden
authors.forename: C Roy D
authors.initials: CR
authors.lastname: Lancaster
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism; D001665:Binding Sites; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004579:Electron Transport; D004734:Energy Metabolism; D005560:Formate Dehydrogenases / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D024482:Vitamin K 2 / Q000378:metabolism; D016968:Wolinella / Q000201:enzymology / Q000378:metabolism*
chemical_list: D005650:Fumarates; D024482:Vitamin K 2; C032005:fumaric acid; D006864:Hydrogenase; D005560:Formate Dehydrogenases; D013385:Succinate Dehydrogenase