Characteristic biological effects of itraconazole on L929 fibroblasts and their cell membrane.
Itraconazole (ITCZ), a triazole antifungal agent, was studied for its effects on the morphology and function of L929 fibroblasts. L929 fibroblasts were cultured for 20 h with ITCZ or one of several other triazoles (fluconazole, ketoconazole, and hydroxy-itraconazole [ITCZ-OH]) at the concentration of 0.5 microg/ml. Among these agents, only ITCZ and its metabolite ITCZ-OH markedly elongated the cells bidirectionally. Scanning electron microscopy studies showed that the surface of the elongated cells was smoother than that of the untreated cells. The viability of L929 cells cultured with 0.5 microg/ml of ITCZ for 20 h was not lowered. However, after treatment with 0.0375% sodium deoxycholate (DOC) solution, the viability of the cells treated with ITCZ, as evaluated by the 3-(4,5-dimethyl-2thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) proliferation assay or the release of lactic dehydrogenase from cytoplasm, was decreased. When L929 cells were cultured in the presence of a combination of ITCZ and vincristine, their growth was synergistically inhibited. This synergism was also observed when ITCZ was replaced by ITCZ-OH, but not by the other azoles. These findings suggest that the exposure of L929 fibroblasts to low ITCZ concentrations affects the physiological nature of their cell membrane.
Abe
S
S
Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. sabe@med.teikyo-u.ac.jp
Ochi
H
H
Takahashi
Y
Y
Ishijima
S A
SA
Osumi
M
M
Yamaguchi
H
H
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D066298:In Vitro Techniques; D017964:Itraconazole / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D014750:Vincristine / Q000494:pharmacology
D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D003902:Detergents; D003840:Deoxycholic Acid; D017964:Itraconazole; D014750:Vincristine