Proteomic analysis of human prostate cancer.
Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.
Ahram
Mamoun
M
Pathogenetics Unit, Laboratory of Pathology and Urologic Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Best
Carolyn J M
CJ
Flaig
Michael J
MJ
Gillespie
John W
JW
Leiva
Isabel M
IM
Chuaqui
Rodrigo F
RF
Zhou
Ge
G
Shu
Hungjun
H
Duray
Paul H
PH
Linehan
W Marston
WM
Raffeld
Mark
M
Ornstein
David K
DK
Zhao
Yingming
Y
Petricoin
Emanuel F
EF
Emmert-Buck
Michael R
MR
D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013058:Mass Spectrometry; D009363:Neoplasm Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D014335:Tropomyosin / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D009363:Neoplasm Proteins; D020543:Proteome; D014335:Tropomyosin; D014357:Trypsin