title: In vitro susceptibility testing of levofloxacin and ofloxacin by microtiter plate Alamar blue against multidrug and non multidrug resistant Mycobacterium tuberculosis in Thailand.
abstract: Antituberculous drugs for current therapy of multidrugs resistant tuberculosis (MDR-TB) are limited. The in vitro susceptibility of Mycobacterium tuberculosis (MTB) as well as MDR-TB with other antibiotic drugs were determined in order to find alternative drugs for the treatment of MDR-TB. Forty-seven MDR-TB and 62 MTB of clinical isolates were tested against levofloxacin and ofloxacin. The MDR-TB at the MIC90 of levofloxacin and ofloxcain were 1 microg/ml and 2 microg/ml, respectively. Of these MTB, the MIC90 of both drugs were 0.5 microg/ml and 1 microg/ml, respectively. It seemed that levofloxacin MICs of both MDR-TB and MTB were one dilution less than ofloxcin. The promising activity of ofloxacin and levofloxacin against MDR-TB and MTB suggest that both drugs could be used as second-line drugs for MDR-TB or as a good alternative antituberculous drug for patients who have intolerance to the first line drug.
authors.affiliation: Department of Pathology, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Pracharktam
authors.forename: K
authors.initials: K
authors.lastname: Angkananukool
authors.forename: A
authors.initials: A
authors.lastname: Vibhagool
mesh_terms: D004396:Coloring Agents; D003470:Culture Media; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D015242:Ofloxacin / Q000494:pharmacology*; D010078:Oxazines; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000188:drug therapy; D014966:Xanthenes
chemical_list: D004396:Coloring Agents; D003470:Culture Media; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D064704:Levofloxacin; D015242:Ofloxacin