title: Improved high performance liquid chromatographic determination of ginsenosides in Panax ginseng-based pharmaceuticals using a diol column.
abstract: A reversed-phase high-performance liquid chromatographic assay for the simultaneous quantitative determination of seven ginsenosides, Rb(1), Rb(2), Rc, Rd, Rg(1), Re and Rf in pharmaceutical preparations is described. Chromatographic separation was achieved in less than 20 min using a 250 x 4 mm Lichrospher, 5 microm, 100 A diol column with detection at 203 nm. The method was validated over the range of 2.5-20 ng/microL using a 20 microL sample volume. The average accuracy at five concentrations was 90-100%, and the within-day and between-day precision ranged from 1 to 7% expressed as coefficient of variation. The detection limit and the quantitation limit of the method were 20 and 50 ng injected for each ginsenoside, respectively.
authors.affiliation: Universitat de Barcelona, Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Avda Diagonal no. 643, 08028 Barcelona, Catalonia, Spain. bonfill@farmacia.far.ub.es
authors.forename: Mercè
authors.initials: M
authors.lastname: Bonfill
authors.forename: Isidre
authors.initials: I
authors.lastname: Casals
authors.forename: Javier
authors.initials: J
authors.lastname: Palazón
authors.forename: Anna
authors.initials: A
authors.lastname: Mallol
authors.forename: Carmen
authors.initials: C
authors.lastname: Morales
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides; D005894:Panax / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D036145:Ginsenosides; D004364:Pharmaceutical Preparations; D012503:Saponins