title: Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells.
abstract: Dendritic cells (DC), the most potent APC, are central to antimicrobial immunity. Because of evolutionary pressure, it is reasonable that pathogens have evolved strategies to also subvert this host-defense mechanism. In the present study, we describe a novel way of bacterial interference with DC maturation. The bacterial metabolite n-butyrate, which occurs physiologically in high concentrations in the gastrointestinal tract and has well-known anti-inflammatory effects, is able to prevent LPS-induced maturation of DC resulting in a reduced capability to stimulate T cells. In particular, n-butyrate prevents homotypic DC clustering, inhibits IL-12 while sparing IL-10 production, and at the molecular level, blocks NF-kappa B translocation. These results demonstrate efficient targeting of DC function by a bacterial metabolite, which might explain the particular type of immune responsiveness in the presence of this bacterial agent as exemplified in the gastrointestinal tract.
authors.affiliation: Institute of Immunology, University of Vienna, Austria.
authors.forename: Marcus D
authors.initials: MD
authors.lastname: Säemann
authors.forename: Ornella
authors.initials: O
authors.lastname: Parolini
authors.forename: Georg A
authors.initials: GA
authors.lastname: Böhmig
authors.forename: Peter
authors.initials: P
authors.lastname: Kelemen
authors.forename: Peter-Michael
authors.initials: PM
authors.lastname: Krieger
authors.forename: Josef
authors.initials: J
authors.lastname: Neumüller
authors.forename: Katharina
authors.initials: K
authors.lastname: Knarr
authors.forename: Willibald
authors.initials: W
authors.lastname: Kammlander
authors.forename: Walter H
authors.initials: WH
authors.lastname: Hörl
authors.forename: Christos
authors.initials: C
authors.lastname: Diakos
authors.forename: Karl
authors.initials: K
authors.lastname: Stuhlmeier
authors.forename: Gerhard J
authors.initials: GJ
authors.lastname: Zlabinger
mesh_terms: D000328:Adult; D001419:Bacteria / Q000276:immunology* / Q000378:metabolism; D002087:Butyrates / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007109:Immunity; D009000:Monocytes / Q000166:cytology / Q000276:immunology
chemical_list: D002087:Butyrates