Antimicrobial characteristics of chitosans against food spoilage microorganisms in liquid media and mayonnaise.
Four different kinds of chitosans were prepared by treating crude chitin with various NaOH concentrations. The antimicrobial activities of the chitosans were tested against four species of food spoilage microorganisms (Lactobacillus plantarum, Lactobacillus fructivorans, Serratia liquefaciens, and Zygosaccharomyces bailii). The initial effect of the chitosans was biocidal, and counts of viable cells were significantly reduced. After an extended lag phase, some strains recovered and resumed growth. The activities of chitosan against these microorganisms increased with the concentration. Chitosan-50 was most effective against L. fructivorans, but inhibition of L. plantarum was greatest with chitosan-55. There was no significant difference among the chitosans in their antimicrobial activity against S. liquefaciens and Z. bailii. The addition of chitosan to mayonnaise significantly decreased the viable cell counts of L. fructivorans and Z. bailii during storage at 25 degrees C. These results suggest that chitosan can be used as a food preservative to inhibit the growth of spoilage microorganisms in mayonnaise.
Oh
H I
HI
Department of Food Science and Technology, Sejong University, Seoul, Korea. ohhi@sejong.ac.kr
Kim
Y J
YJ
Chang
E J
EJ
Kim
J Y
JY
D000107:Acetylation; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D048271:Chitosan; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005520:Food Preservatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008970:Molecular Weight; D012705:Serratia / Q000187:drug effects / Q000254:growth & development; D020068:Zygosaccharomyces / Q000187:drug effects / Q000254:growth & development
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005520:Food Preservatives; D002686:Chitin; D048271:Chitosan