title: Cryopreservation of plumular explants of coconut (Cocos nucifera L.) to support programmes for mass clonal propagation through somatic embryogenesis.
abstract: Callus growth has been observed from plumules of ecotype Laguna Tall after cryopreservation using an encapsulation/dehydration protocol. Sucrose preculture treatment and silica gel dehydration both significantly influenced the frequency of callus formation from non-frozen and frozen plumules. The greatest frequency of post-thaw callus growth occurred after incubation of the encapsulated plumules for 72-96 h in medium containing 0.75 M sucrose followed by desiccation over silica gel for 7-8 h down to approximately 30% moisture content (fresh weight basis). Freezing and thawing were carried out rapidly. Post-thaw recovery rates in excess of 80% were recorded.
authors.affiliation: Plant Biotechnology Laboratories, Imperial College at Wye, T.H. Huxley School, Wye, Ashford, Kent, TN25 5AH, UK.
authors.forename: R
authors.initials: R
authors.lastname: Hornung
authors.forename: R
authors.initials: R
authors.lastname: Domas
authors.forename: P T
authors.initials: PT
authors.lastname: Lynch
mesh_terms: D001947:Breeding; D019976:Cloning, Organism; D003059:Cocos; D015925:Cryopreservation; D003470:Culture Media; D046508:Culture Techniques; D012639:Seeds; D013395:Sucrose
chemical_list: D003470:Culture Media; D013395:Sucrose