title: Effect of gravity on localization of chondrocytes implanted in cartilage defects.
abstract: The transplantation of autologous chondrocytes under a periosteal flap has been used to treat focal cartilage defects. Results have been promising but occasionally involve complications ranging from graft hypertrophy to detachment. The objective of this study was to determine if gravitational forces affect the uniformity of cell distribution within the defect. Using an ex vivo bovine model, the orientation relative to gravity of a repaired full-thickness articular cartilage defect was found to affect the initial distribution of transplanted chondrocytes, prelabeled with 3H-thymidine. After 4 hours, the injected cells (3H-radioactivity) were primarily at the base of the defect (79%) in samples oriented in the up position, primarily at the dependent semicylindrical half of the defect (83%) in samples oriented to the side, and primarily at the periosteal top of the defect (78%) in samples oriented upside-down. Subsequently, the cell distribution remained unchanged after reorientation into other positions. These results indicate that injected chondrocytes localize under the influence of gravity within the initial few hours after injection. Therefore, the defect orientation during this time can be an important factor in the uniformity of cell distribution in the autologous chondrocyte implantation procedure and may be an important determinant of the ultimate clinical outcome.
authors.affiliation: Department of Bioengineering, University of California-San Diego, La Jolla 92093-0412, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Sohn
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Lottman
authors.forename: Leon Y
authors.initials: LY
authors.lastname: Lum
authors.forename: Sang G
authors.initials: SG
authors.lastname: Kim
authors.forename: Robert A
authors.initials: RA
authors.lastname: Pedowitz
authors.forename: Richard D
authors.initials: RD
authors.lastname: Coutts
authors.forename: Robert L
authors.initials: RL
authors.lastname: Sah
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002358:Cartilage, Articular / Q000503:physiopathology*; D002417:Cattle; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D004195:Disease Models, Animal; D007592:Joint Diseases / Q000628:therapy; D007719:Knee Joint / Q000503:physiopathology; D011336:Probability; D012680:Sensitivity and Specificity