Immunohistological and morphometric analysis of inflammatory cells in rapidly progressive periodontitis and adult periodontitis.
The purpose of this study was to localize, characterize, and quantify in situ the inflammatory cells in the gingival connective tissue prior and subsequent to the initial therapy of ten patients with rapidly progressive periodontitis (RPP) and five patients with adult periodontitis (AP). Using immunohistological techniques, the amount of T lymphocytes, alphabeta-T lymphocytes, gammadelta-T lymphocytes, B lymphocytes, and plasma cells was determined at the beginning of the periodontal therapy (baseline) and at the time of periodontal surgery. Furthermore, the distribution of collagen types I, III, V, and VI was investigated using transmission electron microscopy. At baseline, patients with RPP revealed much higher numbers of inflammatory cells than patients with AP. During initial therapy of patients with RPP, the amount of T cells, alphabeta-T cells, and gammadelta-T cells was reduced significantly (P<0.05). Biopsies of patients with AP revealed a statistically significant reduction of all cell types, except alphabeta-T cells and gammadelta-T cells in the deep connective tissue. The transmission electron microscopy of biopsies from patients with RPP and AP with severe inflammation taken at baseline revealed that collagen types I and III were destroyed nearly completely in areas with leukocyte infiltration, whereas collagen types V and VI revealed a more pronounced labeling reaction. The results revealed that, during initial therapy, the amount of inflammatory cells was reduced significantly more in biopsies of patients with AP than in patients with RPP. At baseline, the inflamed gingival tissue consists mainly of collagen types V and VI in areas with infiltrates of inflammatory cells.
Hillmann
G
G
Department of Conservative Dentistry and Periodontology, Dental School, Medical University of Hannover, Germany. Hillmann.Georg@MH-Hannover.de
Krause
S
S
Ozdemir
A
A
Dogan
S
S
Geurtsen
W
W
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology; D001706:Biopsy; D002634:Chemotaxis, Leukocyte; D024042:Collagen Type I / Q000648:ultrastructure; D024061:Collagen Type III / Q000648:ultrastructure; D024062:Collagen Type V / Q000648:ultrastructure; D024142:Collagen Type VI / Q000648:ultrastructure; D003238:Connective Tissue / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000473:pathology; D005891:Gingivitis / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000145:classification / Q000473:pathology*; D008297:Male; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D010518:Periodontitis / Q000145:classification / Q000473:pathology* / Q000601:surgery / Q000628:therapy; D010950:Plasma Cells / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013601:T-Lymphocytes / Q000473:pathology
D024042:Collagen Type I; D024061:Collagen Type III; D024062:Collagen Type V; D024142:Collagen Type VI; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta