Correlation of field-measured toxicity with chemical concentration and pollutant availability.
Direct field toxicity tests were performed in two Louisiana waterways, Bayous Trepagnier and St. John, on sediments containing organic/heavy metal mixtures. Our approach involved bioluminescent bacterial toxicity assays (using DeltaTox, which qualitatively identifies polluted areas, and Microtox, which quantifies toxicity). Samples were more completely analyzed in our laboratory using inductively coupled plasma atomic emission spectroscopy (ICP-AES) and gas chromatography/mass spectrometry (GC/MS). Results indicate that lead is the primary toxic metal at the sites examined, though concentrations of metals fluctuate due to spatial variation and the dynamic nature of the waterways. Polycyclic aromatic hydrocarbons (PAHs) are the most abundant group of organics measured and appear to contribute to the overall toxic response. DeltaTox located toxic hotspots where there was an average light loss of 53-100%. Toxicity results from both assays agree but are well correlated with concentration measurements only for certain sediment fractions. Overall, the DeltaTox/Microtox approach appears to be rapid and cost effective for on-site hotspot identification, and may increase understanding of hazards associated with heavy metal and organic contaminants in these waterways.
Mowat
F S
FS
Department of Biomedical Engineering and Centerfor Bioenvironmental Research, Tulane University, New Orleans, LA 70118, USA.
Bundy
K J
KJ
D001681:Biological Assay / Q000379:methods; D001682:Biological Availability; D004784:Environmental Monitoring; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000737:chemistry; D008163:Luminescent Measurements; D019216:Metals, Heavy / Q000493:pharmacokinetics / Q000633:toxicity*; D018675:Toxicity Tests; D014733:Vibrio / Q000187:drug effects / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
D019216:Metals, Heavy; D014874:Water Pollutants, Chemical