Production of linolenic acid by Mortierella isabellina grown on octadecanol.
The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23 degrees C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitable concentration of Mg2+ in the medium caused an increase in mycelial mass as well as linolenic acid production; (ii) when incubated at 23 degrees C, maximal linolenic acid productivity was reached, although a higher content of the acid in total fatty acids was found at the lower temperature; (iii) the effect of substrate concentration on linolenic acid yield showed that the latter increased with concentration of substrate, and maximal linolenic acid yield was obtained with concentrations of 2% octadecanol and 1% yeast extract.
Xian
Mo
M
Department of Chemistry, Jilin University, Changchun 130023, China. byl@mail.jlu.edu.cn
Kang
Yijian
Y
Yan
Jicang
J
Liu
Junhong
J
Bi
Yingli
Y
Zhen
Kaiji
K
D002851:Chromatography, High Pressure Liquid; D005233:Fatty Alcohols / Q000378:metabolism*; D020094:Mortierella / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000096:biosynthesis*
D005233:Fatty Alcohols; D017962:alpha-Linolenic Acid