title: [Effect of hypoxia on proliferation and differentiation of the pulmonary vascular pericytes].
abstract: OBJECTIVE
  To study the effect of hypoxia on proliferation and differentiation of the pulmonary vascular pericytes (PC).
  
  
  METHODS
  Cell culture, (3)H-thymidine incorporation ((3)H-TdR), immunocytochemistry and image analysis methods were used.
  
  
  RESULTS
  It was found that the (3)H-thymidine incorporation, the expression amounts of proliferating cell nuclear antigen (PCNA) and alpha-smooth muscle actin (alpha-SM-Actin) in direct hypoxia (H) group was 2.16 times (P < 0.01), 1.16 times(P < 0.01) and 1.11 times (P < 0.05) that of normoxia (N) group, in hypoxic endothelial cell conditioned medium (HECCM) group was 1.8 times (P < 0.01), 1.15 times (P < 0.01) and 1.12 times (P < 0.05) that of normoxic endothelial cell conditioned medium (NECCM) group.
  
  
  CONCLUSIONS
  It was suggested that hypoxia can stimulate pulmonary artery endothelial cells (PAEC) to secrete some growth factors to enhance PC proliferation and transformation of smooth muscle-like cells.
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Xiong
authors.forename: D
authors.initials: D
authors.lastname: Che
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.forename: S
authors.initials: S
authors.lastname: Liu
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006976:Hypertension, Pulmonary / Q000209:etiology; D020286:Pericytes / Q000166:cytology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011651:Pulmonary Artery / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine
chemical_list: D000199:Actins; D018809:Proliferating Cell Nuclear Antigen