title: A simple formula useful for positional cloning.
abstract: We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and hence should be useful for planning positional cloning experiments. The predictions of this formula agree well with the results from a number of published experiments in Arabidopsis.
authors.affiliation: Department of Mathematics, Cornell University, Ithaca, New York 14853-4201, USA.
authors.forename: Richard T
authors.initials: RT
authors.lastname: Durrett
authors.forename: Kai-Yi
authors.initials: KY
authors.lastname: Chen
authors.forename: Steven D
authors.initials: SD
authors.lastname: Tanksley
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005821:Genetic Techniques; D008957:Models, Genetic; D015233:Models, Statistical