[The effect of bombesin on cyclin D1/CDK4 of immortalized human gastric epithelial cell line].
OBJECTIVE
To investigate the regulatory effect of bombesin on the growth of immortalized human gastric epithelial cell line GES-1, as well as on the cell cycle-regulating elements cyclin D1 and CDK4.


METHODS
GES-1 cells were treated with bombesin, alone or together with an bombesin antagonist. The growth effect was evaluated by MTT method, cyclin D1 expression by flow cytometry, CDK4 expression by Western blot plus enhanced chemoluminescence and CDK4 activity by immunoprecipitation with antibody-coated Sepharose beads.


RESULTS
Bombesin at 10(-7) mol/L significantly stimulated the growth of GES-1 cells, increased cyclin D1 expression which was inhibited by bombesin antagonist. Bombesin also increased CDK4 protein expression and CDK4 activity.


CONCLUSIONS
In an immortalized human gastric epithelial cell line, bombesin promotes cell cycle progression via an increase in the expression of cyclin D1/CDK4 and CDK4 activity.
Li
J
J
Department of Gastroenterology, Peking Union Medical College Hospital, Beijing 100730, China.
Chen
Y
Y
Qian
J
J
Chen
Y
Y
Sun
T
T
Ke
Y
Y
D001839:Bombesin / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D019938:Cyclin D1 / Q000378:metabolism*; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans
D019938:Cyclin D1; D051358:Cyclin-Dependent Kinase 4; D001839:Bombesin