title: Differential activation of influenza A virus endonuclease activity is dependent on multiple sequence differences between the virion RNA and cRNA promoters.
abstract: Influenza virus endonuclease activity was studied in vitro with model virion RNA (vRNA) and cRNA molecules. We show that endonuclease activity can be partially rescued by transplanting vRNA-like promoter features into the model cRNA promoter. This study defines three distinctive features within the vRNA promoter--absent in the cRNA promoter--that are required for endonuclease cleavage.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Leahy
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Zecchin
authors.forename: George G
authors.initials: GG
authors.lastname: Brownlee
mesh_terms: D001483:Base Sequence; D004720:Endonucleases / Q000378:metabolism*; D004789:Enzyme Activation; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology* / Q000235:genetics; D007251:Influenza, Human / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018075:RNA, Complementary / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014771:Virion / Q000235:genetics*
chemical_list: D018075:RNA, Complementary; D012367:RNA, Viral; D004720:Endonucleases