Hydration effects of heparin on antithrombin probed by osmotic stress.
Antithrombin is a key inhibitor of blood coagulation proteases and a prototype metastable protein. Heparin binding to antithrombin induces conformational transitions distal to the binding site. We applied osmotic stress techniques and rate measurements in the stopped flow fluorometer to investigate the possibility that hydration changes are associated with these transitions. Water transfer was identified from changes in the free energy of activation, Delta G(++), with osmotic pressure pi. The Delta G(++) was determined from the rate of fluorescence enhancement/decrease associated with heparin binding/release. The volume of water transferred, Delta V, was determined from the relationship, Delta G/pi = Delta V. With an osmotic probe of 4 A radius, the volumes transferred correspond to 158 +/- 11 water molecules from reactants to bulk during association and 162 +/- 22 from bulk to reactants during dissociation. Analytical characterization of water-permeable volumes in x-ray-derived bound and free antithrombin structures were correlated with the volumes measured in solution. Volume changes in water permeable pockets were identified at the loop-insertion and heparin-binding regions. Analyses of the pockets' atomic composition indicate that residues Ser-79, Ala-86, Val-214, Leu-215, Asn-217, Ile-219, and Thr-218 contribute atoms to both the heparin-binding pockets and to the loop-insertion region. These results demonstrate that the increases and decreases in the intrinsic fluorescence of antithrombin during heparin binding and release are linked to dehydration and hydration reactions, respectively. Together with the structural analyses, results also suggest a direct mechanism linking heparin binding/release to loop expulsion/insertion.
McGee
Maria P
MP
Wake Forest University Medical School, Medicine Department, Medical Center Boulevard, Winston-Salem, North Carolina 27157, USA. mmcgee@wfubmc.edu
Liang
Jie
J
Luba
James
J
D000409:Alanine / Q000737:chemistry; D000991:Antithrombins / Q000737:chemistry* / Q000378:metabolism; D001216:Asparagine / Q000737:chemistry; D001665:Binding Sites; D006493:Heparin / Q000737:chemistry / Q000378:metabolism*; D007532:Isoleucine / Q000737:chemistry; D007700:Kinetics; D007930:Leucine / Q000737:chemistry; D008958:Models, Molecular; D009997:Osmotic Pressure; D011485:Protein Binding; D012694:Serine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D013912:Threonine / Q000737:chemistry; D013997:Time Factors; D014364:Tryptophan / Q000737:chemistry; D014633:Valine / Q000737:chemistry; D014867:Water / Q000737:chemistry / Q000378:metabolism*; D014965:X-Rays
D000991:Antithrombins; D007532:Isoleucine; D014867:Water; D013912:Threonine; D012694:Serine; D001216:Asparagine; D014364:Tryptophan; D006493:Heparin; D007930:Leucine; D014633:Valine; D000409:Alanine