Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration.
We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca(2+)](i) and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCepsilon to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.
Throckmorton
Doug
D
Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, USA.
Kurscheid-Reich
Doris
D
Rosales
Oscar R
OR
Rodriguez-Commes
Jose
J
Lopez
Raquel
R
Sumpio
Bauer
B
Zhong
Qing
Q
Ding
Ke Hong
KH
McCarthy
Richard
R
Barrett
Paula Q
PQ
Isales
Carlos M
CM
D000331:Aequorin / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002460:Cell Line; D002518:Ceramides / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D010281:Parathyroid Hormone / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000096:biosynthesis / Q000737:chemistry; D021381:Protein Transport; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
D015220:Calcium Channels; D002518:Ceramides; D004075:Diglycerides; D010281:Parathyroid Hormone; D010455:Peptides; D010743:Phospholipids; D020033:Protein Isoforms; D001464:Barium; D000331:Aequorin; D011493:Protein Kinase C; D011188:Potassium; D002118:Calcium