BRCA1 gene sequence variation in centenarians.
With the ample gene sequence information that has become available with the human genome project virtually completed, it has become possible to identify functional gene variants and their frequencies in elderly populations with different aging-related characteristics. Such a genetic epidemiological approach could lead to new insights with respect to the basic mechanisms of aging and longevity as well as the identification of new targets to prevent or retard some of the late-age adverse effects. Using our recently developed two-dimensional gene scanning (TDGS) technology platform we demonstrate the feasibility of this approach by screening two different populations of centenarians for polymorphic variation in the BRCA1 breast cancer susceptibility gene, one of the many genes involved in genome maintenance. The initial results obtained with this approach suggest differences in BRCA1 genotype frequencies between the centenarian populations and controls.
Vijg
J
J
University of Texas Health Science Center, San Antonio 78245, USA. vijg@uthscsa.edu
Perls
T
T
Franceschi
C
C
van Orsouw
N J
NJ
D000368:Aged; D000369:Aged, 80 and over; D001057:Apolipoproteins E / Q000235:genetics; D020029:Base Pairing; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics; D004247:DNA / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D005240:Feasibility Studies; D005787:Gene Frequency; D019398:Genes, BRCA1; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008136:Longevity / Q000235:genetics*; D009154:Mutation; D009691:Nucleic Acid Denaturation; D010641:Phenotype; D011110:Polymorphism, Genetic; D012306:Risk; D012680:Sensitivity and Specificity
D001057:Apolipoproteins E; D004247:DNA