A new type of allantoinase in amphibian liver.
Allantoinase and allantoicase are known to form a complex in amphibian liver. In this study, a new type of allantoinase that did not form a complex with allantoicase was found in the amphibian liver. Purified enzyme had a molecular mass of about 44 kDa both in SDS-PAGE and gel-filtrations. The enzyme cross-reacted with anti-sardine allantoinase polyclonal antibody, and it weakly cross-reacted with anti-bullfrog allantoinase polyclonal antibody.
Masuda
W
W
Department of Biochemistry, Kyushu Dental College, Kokura, Kitakyushu, Japan. wmasuda@kyu-dent.ac.jp
Fujiwara
S
S
Noguchi
T
T
D000581:Amidohydrolases / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D003429:Cross Reactions; D005399:Fishes; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011892:Rana catesbeiana / Q000378:metabolism*; D014512:Ureohydrolases / Q000302:isolation & purification
D046911:Macromolecular Substances; D000581:Amidohydrolases; C019659:allantoinase; D014512:Ureohydrolases; C019658:allantoicase