Spectrofluorimetric determination of hydrogen peroxide with 2-hydroxy-1-naphthaldehyde salicyloylhydrazone (HNSH) as the substrate for horseradish peroxidase (HRP).
The oxidation reaction of HNSH with H2O2 under the catalysis of HRP was studied in detail. The possible reaction mechanism was discussed. Under optimum experimental conditions, the oxidized product of HNSH had excitation and emission maxima at 296 and 414 nm, respectively. A study to prove the existence of -O-O-H in polyethylene glycols was carried out. The proposed method was successfully applied to the determination of -O-O-H in polyethylene glycols.
Tang
Bo
B
Department of Chemistry, Shandong Normal University, Jinan, PR China. toplet@jn-public.sd.cninfo.net
Wang
Yan
Y
Sun
Ying
Y
Shen
Han Xi
HX
D002384:Catalysis; D002855:Chromatography, Thin Layer; D006735:Horseradish Peroxidase / Q000737:chemistry; D006835:Hydrazones / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D009281:Naphthalenes / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D020959:Polyethylene / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011193:Potassium Iodide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors
D006835:Hydrazones; D009281:Naphthalenes; D013501:Surface-Active Agents; D011193:Potassium Iodide; D011092:Polyethylene Glycols; C060584:2-hydroxy-1-naphthalaldehyde salicyloylhydrazone; D020959:Polyethylene; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010100:Oxygen