title: Testing of skeletal implant surfaces with human fetal osteoblasts.
abstract: The effect of standard orthopaedic implant materials on osteoblast proliferation and differentiation was investigated using a human osteoblast cell culture system. Human fetal osteoblasts 1.19 were cultured on stainless steel, cobalt-chrome-molybdenum, and commercially pure titanium for 12 days. Tissue culture polystyrene was used as a control. Cell proliferation was measured by electronic cell counting and by a colorimetric proliferation assay. To assess the degree of differentiation, levels of alkaline phosphatase activity, collagen Type I, and osteocalcin production were measured. Osteocalcin gene expression was measured by reverse transcriptase-polymerase chain reaction. Electronic cell counting and proliferation assays showed lower cell numbers and delayed proliferation on stainless steel and cobalt-chrome-molybdenum compared with titanium and polystyrene. Alkaline phosphatase and osteocalcin were measured higher on titanium than on stainless steel or cobalt-chrome-molybdenum. Differences in collagen Type I production were not found. Reverse transcriptase-polymerase chain reaction showed the highest osteocalcin gene expression on titanium. The human fetal osteoblast cell line 1.19 provides a rapidly proliferating and differentiating system for testing biomaterials in which differences in osteoblast proliferation and differentiation on orthopaedic implant materials could be revealed, suggesting that the chemistry of biomaterials has a dynamic effect on proliferation and differentiation of human osteoblasts.
authors.affiliation: Department of Orthopaedic Surgery, Julius Maximilians University, Würzburg, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Hendrich
authors.forename: Ulrich
authors.initials: U
authors.lastname: Nöth
authors.forename: Ulrich
authors.initials: U
authors.lastname: Stahl
authors.forename: Frank
authors.initials: F
authors.lastname: Merklein
authors.forename: Christoph P
authors.initials: CP
authors.lastname: Rader
authors.forename: Norbert
authors.initials: N
authors.lastname: Schütze
authors.forename: Roger
authors.initials: R
authors.lastname: Thull
authors.forename: Rocky S
authors.initials: RS
authors.lastname: Tuan
authors.forename: Jochen
authors.initials: J
authors.lastname: Eulert
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000704:Analysis of Variance; D001672:Biocompatible Materials; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006801:Humans; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D011336:Probability; D019736:Prostheses and Implants; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D001672:Biocompatible Materials; D015675:Osteocalcin; D000469:Alkaline Phosphatase