Sexually dimorphic expression of Gata-2 during mouse gonad development.
We report that Gata-2 is expressed in a sexually dimorphic fashion during mouse gonadogenesis. Gata-2 transcripts accumulate rapidly in the fetal ovary from 11.5 days post coitum (dpc) onwards, but are not detected in the fetal testis throughout the period studied (10.5-15.5 dpc). Ovarian expression of Gata-2 ceases by 15.5 dpc. Examination of ovaries from embryos homozygous for the extreme allele of c-kit(W(e)) (Nature, 335, 88; Cell, 55, 185) demonstrates that ovarian Gata-2 expression is dependent upon the presence of germ cells. Comparative in situ hybridisation using the germ cell marker Oct4 (EMBO J., 8, 2543) indicates that Gata-2 transcripts are restricted to the germ cell lineage at 13.5 dpc.
Siggers
Pam
P
MRC Mammalian Genetics Unit, Harwell, OX11 0RD, Didcot, UK.
Smith
Lee
L
Greenfield
Andy
A
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D050989:GATA2 Transcription Factor; D018507:Gene Expression Regulation, Developmental; D006720:Homozygote; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D050814:Octamer Transcription Factor-3; D010053:Ovary / Q000196:embryology / Q000254:growth & development*; D013737:Testis / Q000196:embryology / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D014157:Transcription Factors