8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine levels in human urine do not depend on diet.
In the present study, we used the method involving HPLC pre-purification followed by gas chromatography with isotope dilution mass spectrometric detection for the determination of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydroguanine (8-oxoGua) in human urine. The mean levels of 8-oxoGua and 8-oxodGuo in the urine samples of the subjects on unrestricted diet were respectively 1.87 nmol/kg 24 h (+/-0.90) and 0.83 nmol/kg 24 h (+/-0.49), and in the case of the groups studied, they did not depend on the applied diet. The sum of the amounts of both compounds in urine can give information about the formation rate of 8-oxoGua in cellular DNA. It is also likely that the levels of modified nucleo-base/side in urine sample are reflective of the involvement of different repair pathways responsible for the removal of 8-oxodGuo from DNA, namely base excision repair (BER) and nucleotide excision repair (NER).
Gackowski
D
D
Department of Clinical Biochemistry, The Ludwik Rydygier Medical University, Bydgoszcz, Poland.
Rozalski
R
R
Roszkowski
K
K
Jawien
A
A
Foksiński
M
M
Olinski
R
R
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine*; D004032:Diet; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009696:Nucleic Acids / Q000378:metabolism
D009696:Nucleic Acids; C024829:8-hydroxyguanine; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine