title: p16 gene methylation in colorectal cancers associated with Duke's staging.
abstract: OBJECTIVE
  To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.
  
  
  METHODS
  Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.
  
  
  RESULTS
  p16 methylation was detected in 42% of the tumors.Dukes'staging was associated with p16 methylation status.p16 methylation occurred more frequently in Dukes'C and D patients (75.9%) than in Dukes'A and B patients (12.1%).
  
  
  CONCLUSIONS
  p16 methylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis.
authors.affiliation: Department of Cell Biology, Shanghai Second Medical University, 280 Chongqing South Road, Shanghai 200025, China. yijing@shsmu.edu.cn
authors.forename: J
authors.initials: J
authors.lastname: Yi
authors.forename: Z W
authors.initials: ZW
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Cang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: B M
authors.initials: BM
authors.lastname: Yu
authors.forename: X M
authors.initials: XM
authors.lastname: Tang
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16 / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction