Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer.
OBJECTIVE
To investigate SBA2 expression in CRC cell lines and surgical specimens of CRC and autologous healthy mucosa.


METHODS
Reverse transcription-polymerase chain reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different grades of differentiation and 30 clinical samples. Normalization of the results was achieved by simultaneous amplification of beta-actin as an internal control.


RESULTS
In the exponential range of amplification, fairly good linearity demonstrated identical amplification efficiency for SBA2 and beta-actin (82%). Markedly lower levels of SBA2 mRNA were detectable in tumors, as compared with the coupled normal counterparts P<0.01). SBA2 expression was significantly (0.01>P < 0.05) correlated with the grade of differentiation in CRC, with relatively higher levels in well-differentiated samples and lower in poorly-differentiated cases. Of the 9 cases with lymph nodes affected, 78% (7/9) had reduced SBA2 mRNA expression in contrast to 24% (5/21) in non-metastasis samples 0.01>P<0.05).


CONCLUSIONS
SBA2 gene might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological features need further studies.
Li
X G
XG
Key Laboratory of Cell Biology, Ministry of Health of China, China Medical University, Shenyang 110001, Liaoning Province, China.
Song
J D
JD
Wang
Y Q
YQ
D014408:Biomarkers, Tumor; D002454:Cell Differentiation / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D009363:Neoplasm Proteins