title: Characterization of mutations in DNA gyrase and topoisomerase IV Involved in quinolone resistance of Mycoplasma gallisepticum mutants obtained in vitro.
abstract: Mycoplasma gallisepticum enrofloxacin-resistant mutants were generated by stepwise selection in increasing concentrations of enrofloxacin. Alterations were found in the quinolone resistance-determining regions of the four target genes encoding DNA gyrase and topoisomerase IV from these mutants. This is the first description of such mutations in an animal mycoplasma species.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches Avicoles et Porcines, Unité de Mycoplasmologie-Bactériologie, 22440 Ploufragan, France.
authors.forename: A K
authors.initials: AK
authors.lastname: Reinhardt
authors.forename: C M
authors.initials: CM
authors.lastname: Bébéar
authors.forename: M
authors.initials: M
authors.lastname: Kobisch
authors.forename: I
authors.initials: I
authors.lastname: Kempf
authors.forename: A V
authors.initials: AV
authors.lastname: Gautier-Bouchardon
mesh_terms: D042462:4-Quinolones; D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D002645:Chickens; D027081:DNA Gyrase / Q000235:genetics* / Q000378:metabolism; D027101:DNA Topoisomerase IV / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D009174:Mycoplasma / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D042462:4-Quinolones; D000890:Anti-Infective Agents; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase