title: [Effect of physical and chemical factors on Macaca Mulatta's pulp and change of the collagen in pulp].
abstract: OBJECTIVE
  To investigate the change of pulp and its collagen to outside stimuli.
  
  
  METHODS
  51 permanent teeth from 10 adult healthy Macaca Mulatta's monkeys were chosen as experimental teeth. 39 of them were prepared Class V cavities. The teeth were divided into two groups, one was filled with zinc phosphate cement and another group with zinc oxide eugenol. A blank control group of 12 teeth was set at the same time. Just after the operation, 3 d, 7 d, 1 month and 3 months later, the animals were executed. Histologic changes were evaluated by means of HE and collagen staining method.
  
  
  RESULTS
  Local inflammation was the major representation in the pulps of the zinc phosphate cement group from 7 d to 3 months after operation. The percentage of collagen decrease dropped sharply, and there was no inflammation cells infiltration in the pulps of zinc oxide eugenol group from 1 to 3 months after operation. By means of collagen staining method, the morphological characters, distribution and the change of pulpal collagen during a certain pathologic process were clearly represented in histologic slices. The change of collagen appeared earlier than the inflammation cells infiltration.
  
  
  CONCLUSIONS
  Zinc phosphate cement can lead to medium inflammation of the pulp. The change of collagen appears earlier than that of WBC, which can be used as a sensitive indicator.
authors.affiliation: Department of Operative Dentistry, Cariology and Endodontology, Peking University School of Stomatology, Beijing 100081, China.
authors.forename: L
authors.initials: L
authors.lastname: Yuan
authors.forename: L
authors.initials: L
authors.lastname: Yue
authors.forename: X
authors.initials: X
authors.lastname: Mao
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis*; D003782:Dental Pulp / Q000737:chemistry / Q000187:drug effects*; D005260:Female; D008253:Macaca mulatta; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology*; D015036:Zinc Phosphate Cement / Q000494:pharmacology*
chemical_list: D015035:Zinc Oxide-Eugenol Cement; D015036:Zinc Phosphate Cement; D003094:Collagen