CD56+, NKp46+ cell line (MZ93) expressing T-cell and myeloid antigens.
The MZ93 cell line, established from a patient with CML, expressed CD4, CD7, CD13, CD25, CD33, CD34, CD56 and NKp46. The additional karyotype abnormality of the Ph-positive leukemia cells in vivo, 6p+, was also observed in MZ93. The early passages of MZ93 expressed CD3 in the cytoplasm, but the late passages did not. The cells did not express mature NK-markers as expected. The messenger RNAs of CD2 and NKp46 were detected and those of CD3varepsilon and CD3zeta were absent in the cells. Therefore, the cell line has the immunophenotype likely to NK and/or T cell precursor.
Hashimoto
Shigeo
S
First Department of Internal Medicine, School of Medicine, Niigata University, Asahimachi-dori 1, Niigata City 951-8520, Japan.
Toba
Ken
K
Tsuchiyama
Junjiro
J
Abe
Takashi
T
Yano
Toshio
T
Momoi
Akihito
A
Okazuka
Kiyoshi
K
Kanazawa
Naoko
N
Takahashi
Masuhiro
M
Aizawa
Yoshifusa
Y
D015703:Antigens, CD / Q000032:analysis; D015415:Biomarkers; D017252:CD3 Complex / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis*; D003593:Cytoplasm / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology*; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D019002:CD56 Antigen; C112260:NCR1 protein, human; D055627:Natural Cytotoxicity Triggering Receptor 1; D011971:Receptors, Immunologic