title: Core 2 oligosaccharides mediate eosinophil and neutrophil peritoneal but not lung recruitment.
abstract: We have investigated the importance of cell-surface serine- and/or threonine-linked oligosaccharide adhesion molecules synthesized by the Golgi enzyme core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GlcNAcT) in mediating eosinophil trafficking to the lung in studies utilizing C2GlcNAcT-I-deficient mice. The number of bronchoalveolar eosinophils, the number of lung eosinophils, and airway responsiveness to methacholine were not significantly different in C2GlcNAcT-I-deficient compared with wild-type mice sensitized and challenged by inhalation with ovalbumin. C2GlcNAcT-I-deficient mice do not demonstrate defects in neutrophil trafficking to the lung in response to lipopolysaccharide (LPS). In contrast, ragweed-sensitized C2GlcNAcT-I-deficient mice exhibit significantly reduced eosinophil trafficking to the peritoneal cavity in response to ragweed peritoneal challenge. C2GlcNAcT-I-deficient mice also have significantly reduced neutrophil trafficking to the peritoneal cavity in response to LPS challenge. Overall, these studies demonstrate an important role for serine/threonine-linked oligosaccharides synthesized by the Golgi enzyme C2GlcNAcT-I in eosinophil and neutrophil trafficking to the peritoneum but not for eosinophil or neutrophil trafficking to the lung.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla, California 92093, USA. dbroide@ucsd.edu
authors.forename: David H
authors.initials: DH
authors.lastname: Broide
authors.forename: Marina
authors.initials: M
authors.lastname: Miller
authors.forename: Diego
authors.initials: D
authors.lastname: Castaneda
authors.forename: Jyothi
authors.initials: J
authors.lastname: Nayar
authors.forename: Jae Youn
authors.initials: JY
authors.lastname: Cho
authors.forename: Mark
authors.initials: M
authors.lastname: Roman
authors.forename: Lesley G
authors.initials: LG
authors.lastname: Ellies
authors.forename: P
authors.initials: P
authors.lastname: Sriramarao
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology / Q000378:metabolism; D001853:Bone Marrow / Q000201:enzymology; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000378:metabolism; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D002465:Cell Movement / Q000276:immunology*; D004804:Eosinophils / Q000166:cytology* / Q000201:enzymology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006056:Golgi Apparatus / Q000201:enzymology; D008168:Lung / Q000166:cytology / Q000276:immunology; D016210:Methacholine Chloride / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology* / Q000201:enzymology; D009844:Oligosaccharides / Q000378:metabolism*; D010537:Peritoneum / Q000166:cytology / Q000276:immunology; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism; D011014:Pneumonia / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016085:Bronchoconstrictor Agents; D009844:Oligosaccharides; D016210:Methacholine Chloride; D017351:N-Acetylglucosaminyltransferases; C053024:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase