Evidence for a light-induced H(+) conductance in the eye of the green alga Chlamydomonas reinhardtii.
Rhodopsin-mediated photoreceptor currents, I(P), of the unicellular alga Chlamydomonas reinhardtii were studied under neutral and acidic conditions. We characterized the kinetically overlapping components of the first, flash-induced inward current recorded from the eye, I(P1), as a low- and high-intensity component, I(P1a) and I(P1b), respectively. They peak between 1 and 10 ms after the light-flash and are both likely to be carried by Ca(2+). I(P1a) and I(P1b) exhibit half-maximal photon flux densities, Q(1/2), of approximately 0.14 and 58 microE m(-2), and maximal amplitudes of approximately 4.9 and 38 pA, respectively. At acidic extracellular pH values (pH 3-5), both I(P1) currents are followed by distinct H(+) currents, I(P2a) and I(P2b), with maxima after approximately 5 and 100 ms, respectively. Because the Q(1/2) values of I(P1b) and I(P2b) virtually coincide with Q(1/2) of rhodopsin bleaching, we suggest that the respective conductances G(1b) and G(2b) are closely coupled to the rhodopsin, whereas the low light-saturating conductances G(1a) and G(2a) reflect transducer-activated states of a second rhodopsin photoreceptor system.
Ehlenbeck
Sabine
S
Institut für Biochemie I, Universität Regensburg, 93040 Regensburg, Germany.
Gradmann
Dietrich
D
Braun
Franz-Josef
FJ
Hegemann
Peter
P
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002118:Calcium / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D004594:Electrophysiology; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012243:Rhodopsin / Q000378:metabolism; D013816:Thermodynamics
D006859:Hydrogen; D012243:Rhodopsin; D011188:Potassium; D002118:Calcium