Comparative mapping of RPL3, a gene overexpressed in multiple obesity models.
The ribosomal protein 3 gene is differentially expressed in hypothalamus and brown adipose tissue between mouse lines divergently selected for heat loss, and in skeletal muscle of the ob/ob mouse model. Unfortunately, multiple Rpl3-processed pseudogenes have hampered mapping of the functional gene copy in mammalian species. Using PCR amplification with intronic primer binding, we have mapped Rpl3 to MMU15, and have also localized RPL3 to BTA5 in cattle. Comparative mapping implicates a previously mapped copy of RPL3 on HSA22 as the functional copy of human RPL3, while predictive mapping places the porcine homologue on SSC5.
Allan
M F
MF
Animal Science Department, University of Nebraska, Lincoln 68583-0908, USA.
Potts
J K
JK
Pomp
D
D
D000818:Animals; D001833:Body Temperature Regulation / Q000235:genetics; D002874:Chromosome Mapping; D017931:DNA Primers; D004195:Disease Models, Animal; D005784:Gene Amplification; D051379:Mice; D018482:Muscle, Skeletal / Q000502:physiology; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics*
D017931:DNA Primers; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; C000716548:Rpl3 protein, mouse; C429752:ribosomal protein 3