Cryopreservation of axillary buds of a Eucalyptus grandis x eucalyptus camaldulensis hybrid.
This paper presents preliminary results from a study to develop methodology for the cryopreservation of axillary buds from an in vitro hybrid Eucalyptus grandis (W. Hill ex Maiden.) x Eucalyptus camaldulensis (Dehnnh.), maintained for use in a genetic modification programme. Axillary buds were encapsulated in an alginate gel, precultured on media containing elevated levels of sucrose, or a combination of sucrose and glycerol. Encapsulated buds were then dehydrated by evaporation prior to a two-step freezing process in liquid nitrogen. Eighteen percent of shoot explants survived freezing when sucrose alone was used as a protectant against dehydration and cryopreservation. Significantly higher survival (49%) was obtained with the incorporation of glycerol into the protocol. Following cryopreservation, shoots appeared to develop normally, with no evidence of adventitious meristems.
Blakesley
D
D
Department of Biology and Biochemistry, Faculty of Sciences, University of Bath, Claverton Down, UK.
Kiernan
R J
RJ
D015925:Cryopreservation; D005052:Eucalyptus / Q000737:chemistry; D005990:Glycerol / Q000032:analysis; D018520:Plant Shoots / Q000737:chemistry; D017694:Rewarming; D013395:Sucrose / Q000032:analysis
D013395:Sucrose; D005990:Glycerol