Simultaneous determination of endogenous and orally administered (15)N-labeled polyamines in rat organs.
A method for the simultaneous determination of polyamines (putrescine, spermidine, and spermine) by ionspray ionization-mass spectrometry was modified to determine (15)N-labeled polyamines together with unlabeled polyamines using (13)C,(15)N double-labeled polyamines as internal standards. This technique permitted the use of (15)N-labeled polyamines as tracer compounds to follow polyamine biosynthesis, interconversion, and absorption. The method was used to examine the organ distribution of orally administered (15)N-labeled polyamines in rats. Each (15)N-labeled polyamine was taken up by the three organs tested: the small intestine, liver, and kidney. The uptake of (15)N-labeled spermidine was greater than that of (15)N-labeled spermine and putrescine. Administration of a mixture of (15)N-labeled polyamines was useful for determining the disposition of each (15)N-polyamine absorbed from the intestinal tract.
Xu
Yong Ji
YJ
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama, 350-0290, Japan.
Hara
Takeshi
T
Samejima
Keijiro
K
Sasaki
Hitomi
H
Kobayashi
Masaki
M
Takahashi
Aki
A
Niitsu
Masaru
M
D000284:Administration, Oral; D000818:Animals; D007421:Intestine, Small / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D009587:Nitrogen Isotopes / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis* / Q000378:metabolism; D011700:Putrescine / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013095:Spermidine / Q000032:analysis / Q000378:metabolism; D013096:Spermine / Q000032:analysis / Q000378:metabolism
D009587:Nitrogen Isotopes; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine