title: Clinical proof of principle for ChimeriVax: recombinant live, attenuated vaccines against flavivirus infections.
abstract: ChimeriVax is a live, attenuated recombinant virus constructed from yellow fever (YF) 17D in which the envelope protein genes of YF 17D are replaced with the corresponding genes of another flavivirus. A ChimeriVax vaccine was developed against Japanese encephalitis (JE). A randomized, double-blind, outpatient study was conducted to compare the safety and immunogenicity of ChimeriVax-JE and YF 17D. Six YF immune and six non-immune adults were randomized to receive a single SC inoculation of ChimeriVax-JE (5log(10)PFU), ChimeriVax-JE (4log(10)PFU) or YF-VAX((R)) (5log(10)PFU). Mild, transient injection site reactions and flu-like symptoms were noted in all treatment groups, with no significant difference between the groups. Nearly all subjects inoculated with ChimeriVax-JE at both dose levels developed a transient, low-level viremia which was similar in magnitude and duration to that following YF-VAX). Neutralizing antibody seroconversion rates to ChimeriVax-JE was 100% in the high and low dose groups in both naïve and YF immune subjects; seroconversion to wild-type JE strains was similar or lower than to the homologous (vaccine) virus. Mean neutralizing antibody responses were higher in the ChimeriVax-JE high dose groups (naïve subjects LNI 1.55, PRNT(50) 254; YF immune subjects LNI 2.23, PRNT(50) 327) than in the low dose groups (naïve subjects 1.38, PRNT(50) 128; YF immune subjects LNI 1.62, PRNT(50) 270). JE antibody levels were higher in YF immune than in naïve subjects, dispelling concerns about anti-vector immunity. The safety and immunogenicity profile of ChimeriVax-JE vaccine appears to be similar to that of YF 17D. The new vaccine holds promise for prevention of JE in travelers and residents of endemic countries. The ChimeriVax technology platform is being exploited for development of new vaccines against dengue and West Nile.
authors.affiliation: Research and Medical Affairs, Acambis Inc., 38 Sidney Street, Cambridge, MA 02139, USA. thomas.monath@acambis.com
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Monath
authors.forename: Karen
authors.initials: K
authors.lastname: McCarthy
authors.forename: Philip
authors.initials: P
authors.lastname: Bedford
authors.forename: Casey T
authors.initials: CT
authors.lastname: Johnson
authors.forename: Richard
authors.initials: R
authors.lastname: Nichols
authors.forename: Sutee
authors.initials: S
authors.lastname: Yoksan
authors.forename: Ron
authors.initials: R
authors.lastname: Marchesani
authors.forename: Michael
authors.initials: M
authors.lastname: Knauber
authors.forename: Keith H
authors.initials: KH
authors.lastname: Wells
authors.forename: Juan
authors.initials: J
authors.lastname: Arroyo
authors.forename: Farshad
authors.initials: F
authors.lastname: Guirakhoo
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D004311:Double-Blind Method; D005260:Female; D018177:Flavivirus Infections / Q000517:prevention & control*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000009:adverse effects / Q000276:immunology*; D014766:Viremia / Q000517:prevention & control; D022341:Yellow Fever Vaccine / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D022321:Japanese Encephalitis Vaccines; D011993:Recombinant Fusion Proteins; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; D022341:Yellow Fever Vaccine; D000410:Alanine Transaminase