PRINS tandem labeling of satellite DNA in the study of chromosome damage.
Tandem labeling of satellite DNA was proposed a few years ago (1) for evaluating preferential chromosome breaks in the pericentromeric regions of mammalian chromosomes, and (2) for distinguishing chromosome breaks from chromosome segregation errors in interphase cells. In the presence of primers and modified nucleotides, primed in situ labeling (PRINS) tags repetitive DNA sequences, and serves as a useful alternative to fluorescence in situ hybridization (FISH). We developed a two-color method for PRINS tandem labeling of centromeric and pericentromeric sequences. The method, which appears to be more sensitive than FISH, was used to assay micronuclei in mouse splenocytes and early spermatids, and it provided insight into mechanisms of induction of chromosome damage in these cells. We compared the sensitivity of this method and of a different two-color approach, based on simultaneous labeling of centromeric and telomeric sequences.
Russo
Antonella
A
Department of Structural and Functional Biology, University of Insubria, Varese, Italy. russo@bio.unipd.it
D000818:Animals; D002503:Centromere; D002869:Chromosome Aberrations; D004276:DNA, Satellite / Q000737:chemistry*; D051379:Mice; D020401:Primed In Situ Labeling / Q000379:methods*; D012680:Sensitivity and Specificity; D016615:Telomere
D004276:DNA, Satellite