title: A recombinant envelope protein from Dengue virus purified by IMAC is bioequivalent with its immune-affinity chromatography purified counterpart.
abstract: Semi-purified DEN-4 envelope protein, obtained in Pichia pastoris, was capable of generating neutralising and protecting antibodies after immunisation in mice. Here we compared two purification processes of this recombinant protein using two chromatographic steps: immune-affinity chromatography and immobilised metal ion adsorption chromatography (IMAC). The protein purified by both methods produced functional antibodies reflected by titres of haemagglutination inhibition and neutralisation. IMAC could be used as an alternative for high scale purification.
authors.affiliation: División de Vacunas, Centro de Ingeniería Genética y Biotecnología, Apdo 6162, La Habana, Cuba. lisset.hermida@cigb.edu.cu
authors.forename: L
authors.initials: L
authors.lastname: Hermida
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
authors.forename: L
authors.initials: L
authors.lastname: Lazo
authors.forename: C
authors.initials: C
authors.lastname: López
authors.forename: G
authors.initials: G
authors.lastname: Márquez
authors.forename: R
authors.initials: R
authors.lastname: Páez
authors.forename: C
authors.initials: C
authors.lastname: Suárez
authors.forename: R
authors.initials: R
authors.lastname: Espinosa
authors.forename: J
authors.initials: J
authors.lastname: García
authors.forename: G
authors.initials: G
authors.lastname: Guzmán
authors.forename: G
authors.initials: G
authors.lastname: Guillén
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D006385:Hemagglutination Inhibition Tests; D007116:Immunization, Passive; D008670:Metals; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D008670:Metals; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins