[Protective effect of c-fos antisense oligonucleotides on brain damage induced by glutamate].
OBJECTIVE
To investigate the relation between glutamate neurotoxicity and c-fos gene expression.


METHODS
c-fos antisense oligonucleotides (AS ODN) was injected into the right lateral ventricles of 9 SD rats to block the c-fos gene expression in brain tissue. c-fos sense oligonucleotides (S ODN) was used a control. The numbers and morphology of neurons in both cerebral cortex and hippocampal CA1 were detected by MIAS-300 image analysing instrument. c-fos gene expression in brain was observed by immunohistochemical method. The content of water and electrolytes in the brain tissue and Ca(2+) in the synapse were measured.


RESULTS
The c-fos AS ODN blocked the c-fos gene expression and reduced the content of both water and sodium in brain tissue and Ca(2+) in symptosome, thus alleviating the morphological damage in neuron. S ODN did not have such effect.


CONCLUSIONS
c-fos gene expression plays an important role in mediating the effect of glutamate neurotoxicity. Blocking the c-fos gene expression could antagonize glutamate neurotoxicity.
Yue
S
S
Department of Pediatrics, Xiangya Hospital, Xiangya Medical College of Zhongnan University, Changsha 410008, China.
Luo
Z
Z
Feng
D
D
D000818:Animals; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D002118:Calcium / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D018698:Glutamic Acid; D008297:Male; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000627:therapeutic use*; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism; D014867:Water / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D016760:Proto-Oncogene Proteins c-fos; D014867:Water; D018698:Glutamic Acid; D012964:Sodium; D002118:Calcium