Characterisation of soy isoflavones and screening for novel malonyl glycosides using high-performance liquid chromatography-electrospray ionisation-mass spectrometry.
HPLC combined with electrospray ionisation (ESI)-MS and photodiode array detection has been employed to study the isoflavone components of soy. All of the known soy isoflavones separated by HPLC were identified and characterised, and three novel isoflavones were detected and screened out. These minor isoflavones were deduced to be isomers of 6"-O-malonyl isoflavone glycosides, based on the ESI-MS and UV data, in which the malonyl group is attached at a position other than the 6" position of the glycosyl moiety of the molecule. These novel malonyl glycosides are as thermally labile as the 6"-O-malonyl glycosides, being converted into known isoflavone glycosides after heating in aqueous ethanol. The advantages of HPLC-ESI-MS in detection of novel isoflavones from plant extracts are reviewed.
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School of Food Science and Technology, Wuxi University of Light Industry, Huihe Road 170, Wuxi, Jiangsu, 214036, People's Republic of China.
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D012639:Seeds / Q000737:chemistry; D030262:Soybean Proteins / Q000737:chemistry / Q000302:isolation & purification; D013025:Glycine max; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005960:Glucosides; D006027:Glycosides; D007529:Isoflavones; D030262:Soybean Proteins; C459793:6''-O-malonyldaidzin; C086566:glycitein