Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat.
Homoeoalleles of Ncc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon of Aegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmplA locus, which is located on chromosome 1A of T timopheevi, was sequenced and converted to a PCR-based sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T timopheevi and T turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmplA locus would be informative for studies of the differentiation of chromosome 1A in wheat.
Asakura
N
N
Laboratory of Biology, Faculty of Engineering, Kanagawa University, Yokohama, Japan. asakura@cc.kanagawa-u.ac.jp
Mori
N
N
Ishido
T
T
Ohtsuka
I
I
Nakamura
C
C
D000483:Alleles; D001483:Base Sequence; D015415:Biomarkers; D002467:Cell Nucleus / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D015183:Restriction Mapping; D014908:Triticum / Q000235:genetics*
D015415:Biomarkers