Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut.
The objectives of the study were to identify the relevant hydraulic parameters associated with stomatal regulation during water stress and to test the hypothesis of a stomatal control of xylem embolism in walnut (Juglans regia x nigra) trees. The hydraulic characteristics of the sap pathway were experimentally altered with different methods to alter plant transpiration (Eplant) and stomatal conductance (gs). Potted trees were exposed to a soil water depletion to alter soil water potential (Psisoil), soil resistance (Rsoil), and root hydraulic resistances (Rroot). Soil temperature was changed to alter Rroot alone. Embolism was created in the trunk to increase shoot resistance (Rshoot). Stomata closed in response to these stresses with the effect of maintaining the water pressure in the leaf rachis xylem (P(rachis)) above -1.4 MPa and the leaf water potential (Psileaf) above -1.6 MPa. The same dependence of Eplant and gs on P(rachis) or Psileaf was always observed. This suggested that stomata were not responding to changes in Psisoil, Rsoil, Rroot, or Rshoot per se but rather to their impact on P(rachis) and/or Psileaf. Leaf rachis was the most vulnerable organ, with a threshold P(rachis) for embolism induction of -1.4 MPa. The minimum Psileaf values corresponded to leaf turgor loss point. This suggested that stomata are responding to leaf water status as determined by transpiration rate and plant hydraulics and that P(rachis) might be the physiological parameter regulated by stomatal closure during water stress, which would have the effect of preventing extensive developments of cavitation during water stress.
Cochard
Hervé
H
Unité Mixte de Recherche 547 Physiologie Intégrée de l'Arbre Fruitier et Forestier, Institut National de la Recherche Agronomique-Université Blaise Pascal, Site de Crouelle, 63039 Clermont-Ferrand cedex 02, France. cochard@clermont.inra.fr
Coll
Lluis
L
Le Roux
Xavier
X
Améglio
Thierry
T
D000064:Acclimatization / Q000502:physiology; D001696:Biomechanical Phenomena; D029525:Juglandaceae / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D009997:Osmotic Pressure / Q000187:drug effects; D018515:Plant Leaves / Q000378:metabolism / Q000502:physiology; D018517:Plant Roots / Q000378:metabolism / Q000502:physiology; D018547:Plant Stems / Q000378:metabolism / Q000502:physiology; D018514:Plant Structures / Q000378:metabolism / Q000502:physiology*; D018526:Plant Transpiration / Q000187:drug effects* / Q000502:physiology; D012987:Soil / Q000032:analysis; D014867:Water / Q000378:metabolism / Q000494:pharmacology*
D012987:Soil; D014867:Water