Synthesis and application of a chain-terminating dinucleotide mRNA cap analog.
[structure: see text] We describe the synthesis of a chain-terminating mRNA cap dinucleotide and its use in the in vitro transcription of homogeneously capped RNA. Computer modeling strongly indicates that RNA capped with the new compound will be a substrate for cap-dependent translation.
Peng
Zheng-Hong
ZH
Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, Texas 77030, USA.
Sharma
Vivek
V
Singleton
Scott F
SF
Gershon
Paul D
PD
D015226:Dinucleoside Phosphates / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D008780:Methyltransferases / Q000378:metabolism; D008958:Models, Molecular; D014176:Protein Biosynthesis / Q000187:drug effects; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D015226:Dinucleoside Phosphates; D012314:RNA Cap Analogs; D012333:RNA, Messenger; D008156:Luciferases; D008780:Methyltransferases