Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver.
AIM:To improve the cultivation efficiency and yield of human liver cell line CL-1.METHODS:High-density cultivation of CL-1 on microcarriers was carried out with periodic observation of their growth and proliferation. The specific functions of human liver cell were also determined.RESULTS:Cells of CL-1 cell line grew well on microcarrier Cytodex-3 and on the 7th day the peak was reached. The amount of CL-1 cells was 2.13X10(8) and the total amount of albumin synthesis reached 71.23&mgr;g,urea synthesis 23.32mg and diazepam transformation 619.7ug respectively. The yield of CL-1 on microcarriers was 49.3 times that of conventional cultivation. The amounts of albumin synthesis, urea synthesis and diazepam transformation were 39.8 times, 41.6 times and 33.3 times those of conventional cultivation, respec-tively.CONCLUSION:The human liver cell line CL-1 can be cultivated to a high density with Cytodex-3 and has better biological functions. High-density cultivation of CL-1 on microcarriers can act as the biological material of bioartificial liver.
Gao
Yi
Y
Xu
Xiao-Ping
XP
Hu
Huan-Zhang
HZ
Yang
Ji-Zhen
JZ