[Insect juvenile hormone enhancing gene expression in silkworm baculovirus vector system].
5th instar silkworms were infected with recombinant baculovirus containing phytase gene or wild type BmNPV at 48 hr after ecdysis, then treated with 100 ppm Juvenile hormone. It showed that the expression level of phytase gene and polyhedrin gene per silkworm was increased by 30% and 40%, respectively. The LT50 was lengthened for more than 4 h, and the average weight of sick silkworm was increased by 10%. The results indicated that the improvement of expression efficiency of phytase gene and polyhedrin gene was mainly caused by longer time of virus replication in silkworm after the treatment of Juvenile hormone.
Wang
H W
HW
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China.
Zhang
Z F
ZF
Xiao
Q L
QL
Li
W G
WG
He
J L
JL
D010832:6-Phytase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D012831:Bombyx / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors / Q000235:genetics; D007605:Juvenile Hormones / Q000494:pharmacology*; D000077183:Occlusion Body Matrix Proteins; D014162:Transfection; D014764:Viral Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins
D007605:Juvenile Hormones; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D010832:6-Phytase