title: Intramedullary anchoring strength of titanium rod with mixed beta-tricalcium phosphate and fibrin adhesive.
abstract: Histological and biomechanical studies were conducted in Japanese white rabbits to evaluate the effect of mixed beta-tricalcium phosphate (beta-TCP) and fibrin adhesive on bone formation around a titanium rod in femoral bone marrow. The animals received injections of fibrin adhesive with beta-TCP or hydroxyapatite into the femoral bone marrow from the distal end of the femur, where a titanium rod was inserted. Group I received fibrin adhesive only, group II received fibrin adhesive and hydroxyapatite (HA), and group III received fibrin adhesive and beta-TCP. On the examination of nondecalcified specimens, group III showed cross-bridging bone formation between beta-TCP particles at week 8, this being observed earlier than in group II. On mechanical evaluation, group III showed significant differences in maximum pull-out force at week 8, and in modulus of rigidity at week 24, compared with the other groups. On the examination of decalcified specimens, chronic inflammation was clearly identified in group I at week 8, and residual beta-TCP particles were found in group III at week 24. These results suggest that the mixture given to group III filled bone defects around the rod and promoted bone formation at a relatively early stage. Inflammation and delayed absorption of beta-TCP, which appeared to be a reaction of heterologous fibrins, caused delays in bone formation promoted by beta-TCP. Nevertheless, the use of fibrin adhesive appears to be a feasible method for loading powdered beta-TCP.
authors.affiliation: Department of Orthopaedic Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamaeku, Kawasaki, Kanagawa 216-8511, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Oyake
authors.forename: Moroe
authors.initials: M
authors.lastname: Beppu
authors.forename: Shoji
authors.initials: S
authors.lastname: Ishii
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takagi
authors.forename: Masahisa
authors.initials: M
authors.lastname: Takashi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D001860:Bone Plates; D002130:Calcium Phosphates / Q000494:pharmacology*; D004195:Disease Models, Animal; D017886:Durapatite / Q000494:pharmacology; D005260:Female; D005264:Femoral Fractures / Q000473:pathology* / Q000601:surgery*; D005594:Fracture Fixation, Intramedullary / Q000295:instrumentation* / Q000379:methods; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000502:physiology*; D011336:Probability; D011817:Rabbits; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite; D014025:Titanium; C018392:tricalcium phosphate