Preparation of glycosylated amino acid derivatives for glycoprotein synthesis by in vitro translation system.
General preparation of glycosylated amino acylated nucleotide for in vitro peptide synthesis was described. Both O-glycosylated amino acyl nucleotides and C-glycosylated amino acyl nucleotide were synthesized by choosing the appropriate protecting group.
Manabe
Shino
S
RIKEN (The Institute of Physical and Chemical Research) and CREST, Japan Science and Technology Corporation (JST), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Sakamoto
Kimitoshi
K
Nakahara
Yoshiaki
Y
Sisido
Masahiko
M
Hohsaka
Takahiro
T
Ito
Yukishige
Y
D000596:Amino Acids / Q000138:chemical synthesis* / Q000737:chemistry; D002474:Cell-Free System / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D008358:Mannose / Q000737:chemistry; D014176:Protein Biosynthesis; D012694:Serine / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
D000596:Amino Acids; D006023:Glycoproteins; D012694:Serine; D014364:Tryptophan; D005947:Glucose; D008358:Mannose