Ultra-wideband pulses increase nitric oxide production by RAW 264.7 macrophages incubated in nitrate.
The possible effects of ultra-wideband (UWB) pulses on cellular nitric oxide production were tested by measuring nitrite in the medium bathing UWB exposed RAW 264.7 macrophages. A 30 min exposure to 1 ns UWB pulses, repeated at 600 Hz with an estimated SAR of 0.106 W/kg, did not change nitric oxide production by RAW 264.7 cells, with or without stimulation by gamma interferon and lipopolysaccharide. However, when nitrate was added to the medium of stimulated cells, nitric oxide production increased after UWB exposure, indicating a possible action of UWB pulses on induced nitric oxide synthase under certain conditions.
Seaman
Ronald L
RL
McKessonHBOC Clinical and Biological Services, Microwave Bioeffects Branch, U.S. Army Medical Research Detachment, Brooks AFB, Texas 78235-5460, USA. ronald.seaman@brooks.af.mil
Parker
Jill E
JE
Kiel
Johnathan L
JL
Mathur
Satnam P
SP
Grubbs
Teri R
TR
Prol
H Kenneth
HK
D000818:Animals; D002460:Cell Line; D004574:Electromagnetic Fields / Q000009:adverse effects*; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009566:Nitrates / Q000494:pharmacology; D009569:Nitric Oxide / Q000096:biosynthesis*; D009573:Nitrites / Q000378:metabolism; D011994:Recombinant Proteins
D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; D011994:Recombinant Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma