[Sequencing of inhA in drug-resistant Mycobacterium tuberculosis isolates].
OBJECTIVE
To determine and characterize inhA mutations of drug-resistant isolates of Mycobacterium tuberculosis.


METHODS
PCR products of inhA of drug-resistant and susceptible isolates and H(37)R(v) were purified, cloned and analyzed by ABI 377 auto-DNA sequencing system.


RESULTS
11 out of 17 drug-resistant M. Tuberculosis isolates were detected having inhA variations. 10 out of the 11 strains with variations had at least one missense mutation. The variations consisted of point mutation (transition, transversion and deletion). Molecular mechanisms were different from strain to strain. 8 of 13 (62%) strains had both katG and inhA mutations. 18 variation positions and mechanisms different from previous report have been described.


CONCLUSIONS
These results suggested that binding sites decrease caused by inhA point mutations be associated with M. tuberculosis' resistance to INH and EMB.
Zhu
Z
Z
The Attached Xinhua Hospital of Hainan Medical College, Haikou 570311, China.
Shao
H
H
Chen
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D024881:Drug Resistance, Bacterial / Q000235:genetics* / Q000502:physiology; D004977:Ethambutol / Q000493:pharmacokinetics; D006801:Humans; D007538:Isoniazid / Q000493:pharmacokinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004977:Ethambutol; D010088:Oxidoreductases; D010544:Peroxidases; C076136:catalase HPI; C085257:InhA protein, Mycobacterium; D007538:Isoniazid