Expression and functional role of bTRPC1 channels in native endothelial cells.
We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpc1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells.
Antoniotti
Susanna
S
Department of Animal and Human Biology, University of Torino, Italy.
Lovisolo
Davide
D
Fiorio Pla
Alessandra
A
Munaron
Luca
L
D017398:Alternative Splicing; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000918:Antibody Specificity; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002451:Cell Compartmentation / Q000502:physiology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D007150:Immunohistochemistry; D007473:Ion Channels / Q000235:genetics; D017136:Ion Transport / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050052:TRPC Cation Channels
D000906:Antibodies; D015220:Calcium Channels; D007473:Ion Channels; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; C096351:transient receptor potential cation channel, subfamily C, member 1; D016222:Fibroblast Growth Factor 2; D002118:Calcium