Construction and characterization of a full-length HIV-1(92UG001) subtype D infectious molecular clone.
Here we report the construction, sequencing, and biological characterization of a molecular clone of HIV-1(92UG001), a virus representative of subtype D strains circulating in Uganda. The virus produced by the clone has an aggressive syncytium-inducing phenotype, which matches that of the parental virus. This phenotype may be related to duplication of a binding site for a transcription factor, T cell factor 1alpha (TCF-1alpha), in the long terminal repeat of the virus.
Novelli
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P
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Vella
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Oxford
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J
Daniels
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RS
D000328:Adult; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D016679:Genome, Viral; D015726:Giant Cells / Q000821:virology; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D051821:Lymphoid Enhancer-Binding Factor 1; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D014157:Transcription Factors / Q000378:metabolism; D014454:Uganda / Q000453:epidemiology
D004268:DNA-Binding Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D014157:Transcription Factors