[Types of chemokines expressed by murine dendritic cell line].
OBJECTIVE
To analyze the types of chemokines expressed in murine medullary thymic dendritic cell line (MTDC).


METHODS
A MTDC line was established. RT-PCR was used to amplify the cDNA fragments of SDF-1, IP-10, KC, MCP-1, and lymphotactin expressed by MTDC. Nucleotide sequencing and dot blotting analysis were performed to confirm their existence.


RESULTS
Five cDNA fragments SDF-1, KC, IP-10, MCP-1, and lymphotactin, were amplified from MTDC. The existence of SDF-1, KC, and lymphotactin was confirmed by nucleotide wequencing, and that of IP-10, MCP-1, and lymphotactin was confirmed by dot blotting. The supernatant of MTDC exhibited chemoattractant activities to CD(4)(-) approximately CD(8)(-) thymocytes with a chemotactic index (CI) of 3.4.


CONCLUSIONS
There is a lot of chemokines in MTDC, including C-X-C subfamily (SDF-1, KC, and IP-10) CC subfamily (MCP-1), and C subfamily (lymphotactin). The suparnatant of MTDC has moderately chemotactic activities to CD(4)(-) approximately CD(8)(-) thymocytes, consistent with the biological function of SDF-1.
Guo
J
J
Department of Pediatrics, People's Hospital, Peking Unviersity, Beijing 100044, China.
Gong
S
S
Xie
L
L
D000818:Animals; D002460:Cell Line; D018932:Chemokine CCL2 / Q000235:genetics; D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D018925:Chemokines / Q000235:genetics*; D019744:Chemokines, C; D019743:Chemokines, CXC / Q000235:genetics; D002633:Chemotaxis / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D008222:Lymphokines / Q000235:genetics; D051379:Mice; D012795:Sialoglycoproteins / Q000235:genetics; D013601:T-Lymphocytes / Q000187:drug effects; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D054357:Chemokine CXCL10; D054377:Chemokine CXCL12; D018925:Chemokines; D019744:Chemokines, C; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; C475895:Cxcl1 protein, mouse; C515256:Cxcl12 protein, mouse; D016207:Cytokines; D018076:DNA, Complementary; D008222:Lymphokines; D012795:Sialoglycoproteins; C486249:Xcl1 protein, mouse; C018997:lymphotactin; C074302:keratinocyte-derived chemokines