Immunostaining of von Willebrand factor multimers on agarose gels and nitrocellulose filters.
Human von Willebrand factor (VWF) multimeric analysis is commonly performed by agarose gel electrophoresis, electroblotting, and immunoenzymatic staining; however, high molecular weight (HMW) multimers are poorly transferred on nitrocellulose and should be visualized by direct gel staining with radiolabeled anti-VWF antibody and autoradiography or luminography.
Perutelli
Paolo
P
D000469:Alkaline Phosphatase / Q000032:analysis; D001704:Biopolymers / Q000032:analysis; D001797:Blood Protein Electrophoresis; D002863:Chromogenic Compounds / Q000032:analysis; D003101:Collodion; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D003720:Densitometry; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D009580:Nitroblue Tetrazolium / Q000032:analysis; D012680:Sensitivity and Specificity; D014462:Ultrafiltration; D014841:von Willebrand Factor / Q000032:analysis* / Q000737:chemistry
D001704:Biopolymers; D002863:Chromogenic Compounds; D014841:von Willebrand Factor; D009580:Nitroblue Tetrazolium; D003101:Collodion; D000469:Alkaline Phosphatase; D003894:Deamino Arginine Vasopressin