[The effects of n-3 fatty acid, fructose-1, 6-diphosphate and glutamine on mucosal cell proliferation and apoptosis of small bowel graft after transplantation in rats].
OBJECTIVE
To evaluate the effects of n-3  fatty acid (n-3 FA), fructose-1, 6-diphosphate (FDP) and glutamine (GLN) on mucosal cell proliferation and apoptosis of small bowel graft.


METHODS
196 inbred strain Wistar rats underwent heterotopic small bowel transplantation (SBT) as donors and recipients respectively. n-3FA, FDP and GLN were given via gastrolavage as well as venous infusion within total parenteral nutrition (TPN) solution for 10 days before and after surgery. Flow cytometry and in situ cell death detection were used for analysing the changes of mucosal cell proliferation and apoptosis.


RESULTS
Fewer proliferation and more apoptosis took place in mucosal cells of small bowel graft after transplantation. A higher mucosal cell proliferative index and lower apoptotic index were found in all small bowel graft after supplying with n-3FA, FDP and GLN.


CONCLUSIONS
n-3FA, FDP and GLN specific nutritional support significantly promoted mucosal cell proliferation, and on the other hand inhibited mucosal cell from undergoing apoptosis with different degrees. The effects of regulation help improve the structure and absorption function of transplanted small bowel.
Wu
X
X
Institute of General Surgery, Nanjing General Hospital, People's Liberation Army, Nanjing 210002.
Li
J
J
Li
N
N
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005635:Fructosediphosphates / Q000494:pharmacology*; D005973:Glutamine / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology / Q000637:transplantation*; D008297:Male; D010289:Parenteral Nutrition, Total; D051381:Rats; D017208:Rats, Wistar; D014184:Transplantation, Homologous
D015525:Fatty Acids, Omega-3; D005635:Fructosediphosphates; D005973:Glutamine; C029063:fructose-1,6-diphosphate