Genomic organization of the genes Gtf2ird1, Gtf2i, and Ncf1 at the mouse chromosome 5 region syntenic to the human chromosome 7q11.23 Williams syndrome critical region.
We have recently isolated a mouse ortholog of human GTF2IRD1 that is related to GTF2I. GTF2IRD1 and GTF2I proteins are characterized by the presence of multiple helix-loop-helix domains and a leucine zipper motif. Both paralogs are closely linked and deleted hemizygously in individuals with Williams syndrome, a dominant genetic condition characterized by unique neurocognitive and behavioral features. We have isolated and analyzed the sequence of bacterial artificial chromosome clones from the syntenic mouse chromosome 5 region that contains Gtf2ird1 and Gtf2i as well as a neighboring gene, Ncf1. Gtf2ird1 is composed of 31 exons spanning >100 kb on mouse chromosome 5 and is located between Cyln2 and Gtf2i. Gtf2i is composed of 34 exons spanning about 77 kb. Ncf1, located downstream of Gtf2i, consists of 11 exons that extend over 8 kb. The gene organization of Gtf2ird1, Gtf2i, and Ncf1 is conserved in mice and humans, although the intronic regions are more compact in the mouse genome. The helix-loop-helix repeats of Gtf2ird1 and Gtf2i are encoded separately on adjacent exons and were generated by independent genomic rearrangements. These studies contribute to our knowledge of transcription factor defects and their pathogenesis in haploinsufficiency conditions.
Bayarsaihan
Dashzeveg
D
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Dunai
Judit
J
Greally
John M
JM
Kawasaki
Kazuhiko
K
Sumiyama
Kenta
K
Enkhmandakh
Badam
B
Shimizu
Nobuyoshi
N
Ruddle
Frank H
FH
D000595:Amino Acid Sequence; D000818:Animals; D005091:Exons; D016678:Genome; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D019255:NADPH Oxidases; D010750:Phosphoproteins / Q000235:genetics*; D016415:Sequence Alignment; D026801:Synteny; D014157:Transcription Factors / Q000235:genetics*; D019473:Transcription Factors, TFII; D018980:Williams Syndrome / Q000235:genetics
C104259:GTF2I protein, human; C486330:Gtf2i protein, mouse; D010750:Phosphoproteins; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1