[Protect intercellular communication of pigment epithelium after laser photocoagulation].
OBJECTIVE
To observe the protective effect on the intercellular communication of retinal pigment epithelial (RPE) cells of dexamethasone (Dex) and verapamil (Ver) at different concentrations after laser photocoagulation.


METHODS
Ten pig eyes were used to culture pig pigment epithelium cells. Use different concentrations of Dex and Ver to incubate cultured RPE cells 2 hours before laser coagulation, and then measure the intercellular communication function of confluent cells surrounding the coagulation spots after photocoagulation.


RESULTS
125 mg/L Dex and 20 mg/L Ver could protect the intercellular communication of RPE cells under laser photocoagulation. The high concentration of drugs had better result.


CONCLUSIONS
Both Dex and Ver can preserve the intercellular communication in vitro. The protective capacity is related to the concentration of drug.
He
S
S
Department of Ophthalmology, PLA General Hospital, Beijing 100853.
Wang
F
F
Gu
Z
Z
Huang
J
J
Li
N
N
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D017075:Laser Coagulation / Q000009:adverse effects; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000293:injuries; D012160:Retina / Q000166:cytology*; D013552:Swine; D014700:Verapamil / Q000494:pharmacology*
D003907:Dexamethasone; D014700:Verapamil