title: Ribosomal mutations in Streptococcus pneumoniae clinical isolates.
abstract: Eleven clinical isolates of Streptococcus pneumoniae, isolated in Finland during 1996 to 2000, had an unusual macrolide resistance phenotype. They were resistant to macrolides and streptogramin B but susceptible, intermediate, or low-level resistant to lincosamides. No acquired macrolide resistance genes were detected from the strains. The isolates were found to have mutations in domain V of the 23S rRNA or ribosomal protein L4. Seven isolates had an A2059C mutation in two to four out of the four alleles encoding the 23S rRNA, two isolates had an A2059G mutation in two alleles, one isolate had a C2611G mutation in all four alleles, and one isolate had a 69GTG71-to-69TPS71 substitution in ribosomal protein L4.
authors.affiliation: Antimicrobial Research Laboratory, National Public Health Institute, Turku, Finland. mapihla@utu.fi
authors.forename: Marja
authors.initials: M
authors.lastname: Pihlajamäki
authors.forename: Janne
authors.initials: J
authors.lastname: Kataja
authors.forename: Helena
authors.initials: H
authors.lastname: Seppälä
authors.forename: John
authors.initials: J
authors.lastname: Elliot
authors.forename: Maija
authors.initials: M
authors.lastname: Leinonen
authors.forename: Pentti
authors.initials: P
authors.lastname: Huovinen
authors.forename: Jari
authors.initials: J
authors.lastname: Jalava
mesh_terms: D004351:Drug Resistance / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D011008:Pneumococcal Infections / Q000382:microbiology*; D012335:RNA, Ribosomal; D012270:Ribosomes / Q000235:genetics*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal