A site-specific low-enrichment (15)N,(13)C isotope-labeling approach to unambiguous NMR spectral assignments in nucleic acids.
We demonstrate that base and sugar protons within a DNA oligomer can be assigned unambiguously following site-specific 15N,13C isotope incorporation at levels as low as 1% enrichment. This simple and cost-effective methodology is demonstrated on the d(GGGTTCAGG) DNA sequence, which forms a dimeric G-quadruplex containing G.G.G.G tetrads sandwiched between G.(C-A) triads.
Phan
Anh Tuân
AT
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center New York, New York 10021, USA. phantuan@sbnmr1.mskcc.org
Patel
Dinshaw J
DJ
D002247:Carbon Isotopes / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D009587:Nitrogen Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009943:Organophosphorus Compounds / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA