title: Simple procedure for rapid identification of Vibrio cholerae from the aquatic environment.
abstract: Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae. This technique provided 100% sensitivity and > or =70% specificity.
authors.affiliation: Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt Street, Baltimore, MD 21202.
authors.forename: Nipa
authors.initials: N
authors.lastname: Choopun
authors.forename: Valérie
authors.initials: V
authors.lastname: Louis
authors.forename: Anwar
authors.initials: A
authors.lastname: Huq
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D003470:Culture Media; D010461:Peptones; D010933:Plankton / Q000382:microbiology; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D010461:Peptones