Differentiation of Trypanosoma rangeli: high production of infective Trypomastigote forms in vitro.
In the present study, we report a simple method to induce high Trypanosoma rangeli differentiation in vitro, producing a large number of infective trypomastigote forms. Parasites from SC-58 (Brazil) and Choachi (Colombia) strains were cultivated at 27 degrees C in TC-100, Grace and DMEM media, each supplemented with 5% fetal bovine serum and prepared at three distinct pHs (6.0, 7.0, 8.0). Differentiation was microscopically evaluated at 0, 3 and 6 days after cultivation in each medium by determining the percentage of trypomastigotes in Giemsa-stained smears. Our data revealed similar results for both T. rangeli strains, showing (after 6 days of cultivation in DMEM medium, pH 8.0) the presence of about 80% of trypomastigotes. These culture-derived trypomastigotes proved to be infective to both Balb-C mice and Rhodnius spp, reaching the triatomine's salivary glands. Our results describe a new and easy method to induce high T. rangeli differentiation in vitro, allowing further studies on the antigenic constitution of trypomastigotes.
Koerich
Leonardo Barbosa
LB
Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Emmanuelle-Machado
Priscilla
P
Santos
Kelly
K
Grisard
Edmundo C
EC
Steindel
Mário
M
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D010274:Parasitology / Q000379:methods*; D012239:Rhodnius / Q000469:parasitology; D012469:Salivary Glands / Q000469:parasitology; D014345:Trypanosoma / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D014352:Trypanosomiasis / Q000469:parasitology
D003470:Culture Media