[Experimental study on carcinogenic activity of the organic extracts from water of the sifangtai section of songhua river].
OBJECTIVE
To study the carcinogenic activity and potential mechanism of the organic extracts from the Songhua River in vitro.


METHODS
The in vitro cell transformation and single cell gel electrophoresis (SCGE) tests were detected with the ether extracts of water. The expression of anticancer genes p53, p16, and p21 measured by immunohistochemical assays and the apoptosis by TUNEL assay were also studied.


RESULTS
The extracts from the water induced malignant transformation of BALB/C mouse embryo fibroblast cells and induce DNA damage. p53, p16 and p21 failed to express in transformed cells induced by the water extracts, and the apoptosis rate of transformed cells decreased.


CONCLUSIONS
This study demonstrates that the organic extracts from the Songhua River has potential carcinogenicity.
Fan
C
C
Department of Environmental Health, Harbin Medical University, Harbin 150001, China.
Yang
Y
Y
Tong
Y
Y
D000818:Animals; D017209:Apoptosis; D002273:Carcinogens / Q000633:toxicity*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000378:metabolism; D002478:Cells, Cultured; D002681:China; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004249:DNA Damage; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020868:Gene Silencing / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D002273:Carcinogens; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016159:Tumor Suppressor Protein p53; D014874:Water Pollutants, Chemical