title: Recurrent involvement of the REL and BCL11A loci in classical Hodgkin lymphoma.
abstract: Comparative genomic hybridization studies have shown gains in chromosome region 2p as the most common imbalance in classical Hodgkin lymphoma (cHL). The minimal region of gain contained 2 candidate oncogenes, REL and BCL11A. This study examined the involvement of REL and BCL11A loci in 44 primary cases of cHL by combined immunophenotyping and interphase cytogenetics (FICTION). A median 2p13 copy number above the tetraploid range was detected in 24 (55%) cases. Adjustment for centromere 2 copy number indicated gains of 2p13 in 11 of 31 cHLs (35%) with 8 (26%) high-level amplifications. One cHL displayed selective amplification of the REL locus not affecting BCL11A; another case studied by FICTION and a cHL with cytogenetic 2p change investigated by fluorescence in situ hybridization showed signal patterns suggesting breakpoints in the region spanned by the REL probe. These data indicate that REL rather than BCL11A may be the target of the 2p13 alterations in cHL.
authors.affiliation: Institute of Human Genetics, University Hospital Kiel, Germany.
authors.forename: José I
authors.initials: JI
authors.lastname: Martín-Subero
authors.forename: Stefan
authors.initials: S
authors.lastname: Gesk
authors.forename: Lana
authors.initials: L
authors.lastname: Harder
authors.forename: Takashi
authors.initials: T
authors.lastname: Sonoki
authors.forename: Philip W
authors.initials: PW
authors.lastname: Tucker
authors.forename: Brigitte
authors.initials: B
authors.lastname: Schlegelberger
authors.forename: Werner
authors.initials: W
authors.lastname: Grote
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Novo
authors.forename: María J
authors.initials: MJ
authors.lastname: Calasanz
authors.forename: Martin L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: Martin J S
authors.initials: MJ
authors.lastname: Dyer
authors.forename: Reiner
authors.initials: R
authors.lastname: Siebert
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002352:Carrier Proteins; D002648:Child; D019457:Chromosome Breakage; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D005260:Female; D018628:Gene Dosage; D020573:Genes, rel / Q000235:genetics*; D006689:Hodgkin Disease / Q000209:etiology / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D012097:Repressor Proteins
chemical_list: C486563:BCL11A protein, human; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins