[Culture of chondrocytes seeded onto chitin scaffoldings coating with different materials: experimental study].
OBJECTIVE
To find the possibility of chitin used as scaffolds in tissue engineering.


METHODS
Chondrocytes were cultured when they were seeded onto chitin meshcoated with polylactic acid (PLA) lecithin (LEC) and poly-l-lysine (PLYS). With light microscope and scanning electron microscope, the scaffolds in hydrophilia and absorptivity to chondrocytes and the function of the cells were observed.


RESULTS
LEC can change the hydrophilia of scaffolds. PLYS can strengthen the absorptivity of the scaffolds. and promote the matrices production of chondrocytes.


CONCLUSIONS
The chitin coated with LEC and PLYS can be used as scaffolds in tissue engineering.
Liu
Y
Y
Department of Plastic Surgery, Shanghai Ninth Hospital, Shanghai Second Medical University, Shanghai 200011.
Wang
W
W
Cao
Y
Y
Shang
Q
Q
Zhong
W
W
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002686:Chitin / Q000378:metabolism* / Q000494:pharmacology; D019902:Chondrocytes / Q000166:cytology*; D046508:Culture Techniques / Q000379:methods; D005260:Female; D019344:Lactic Acid / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism / Q000494:pharmacology; D011091:Polyesters; D011107:Polylysine / Q000378:metabolism / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits
D010713:Phosphatidylcholines; D011091:Polyesters; D011108:Polymers; D002686:Chitin; D011107:Polylysine; D019344:Lactic Acid; C033616:poly(lactide)