[Production and characterization of monoclonal antibodies directed agains the major outer membrane protein and lipopolysaccharide of Chlamydia trachomatis].
Two monoclonal antibodies (MnAb) directed against the lipopolysaccharide (LPS) and major outer membrane protein (MOMP) of Chlamydia trachomatis were produced for use in indirect immunofluorescence (IFI). The specificity of the antibodies was determined by Dot-blot, Immunoblotting (IB) and IFI onto culture cells infected with C. trachomatis and IFI onto commercial swabs (MRL). The MnAb 2D3 and 3C2 detected LPS and MOMP of C. trachomatis, respectively, by different methods. Neither MnAb showed cross-reactions when other gram-negative bacteria were used as antigens.
Cuffini
C
C
Laboratorio de Inmunología, Instituto de Virología, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Calle Enfermera Gordillo, Ciudad Universitaria, Agencia 4, 5000 Córdoba, Argentina. ccuffini@cmefcm.uncor.edu.ar
Becher
S
S
Adamo
P
P
Pedranti
M
M
Zapata
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006090:Gram-Negative Bacteria / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting / Q000379:methods; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018272:Porins / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis