title: Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex.
abstract: Histone N-terminal tails are post-translationally modified in many ways. At lysine residues, histones can be either acetylated or methylated. Both modifications lead to the binding of specific proteins; bromodomain proteins, such as GCN5, bind acetyl lysines and the chromodomain protein, HP1, binds methyl lysine 9 of histone H3. Here we show that the previously characterized transcriptional repressor complex NuRD (nucleosome remodeling and deacetylase) binds to the histone H3 N-terminal tail and that methylation at lysine 4, but not lysine 9, prevents binding. Given that lysine 4 methylation is found at sites of active transcription, these results suggest that a function of lysine 4 methylation is to disrupt the association of histones with a repressor complex.
authors.affiliation: Wellcome/Cancer Research UK Institute, Tennis Court Rd., Cambridge, United Kingdom.
authors.forename: Philip
authors.initials: P
authors.lastname: Zegerman
authors.forename: Benito
authors.initials: B
authors.lastname: Canas
authors.forename: Darryl
authors.initials: D
authors.lastname: Pappin
authors.forename: Tony
authors.initials: T
authors.lastname: Kouzarides
mesh_terms: D000107:Acetylation; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006655:Histone Deacetylases / Q000737:chemistry* / Q000378:metabolism*; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000737:chemistry*; D013058:Mass Spectrometry; D008745:Methylation; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D010455:Peptides / Q000737:chemistry; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D010455:Peptides; D006655:Histone Deacetylases; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D008239:Lysine