Quantification of P21 mRNA in biopsy specimens of colon tissue at different stages of neoplastic transformation.
Induction of the P21 protein is an integral part of cell growth arrest associated with the cellular response to senescence or damage. Changes in the P21 protein level are often observed in tumour cells and it is suggested, that they correlate with malignancy. We studied the P21 gene expression at the mRNA level in biopsy specimens from colitis ulcerosa, adenomas and adenocarcinomas using TaqMan assay. Our results showed, that quantity of the P21 transcript was evidently lower in the cases with adenocarcinomas than in the cases with colitis ulcerosa or adenomas.
Dzierzewicz
Z
Z
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Sosnowiec, Poland. zofiadz@farmant.slam.katowice.pl
Kwapisz
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I
Orchel
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Hudyka
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Wilczok
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D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D001706:Biopsy; D002471:Cell Transformation, Neoplastic; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D019914:Taq Polymerase
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D012333:RNA, Messenger; D019914:Taq Polymerase