[Anaerobic infection and its rapid detection in surgical patients].
OBJECTIVE
To investigate anaerobic infection and its rapid detection in surgical patients.


METHODS
372 specimens were collected from surgical patients for anaerobe detection. To find a rapid detectable method, 110 specimens were collected for comparative studies by using indirect immunofluorescent antibody technique (IFA), enzyme labeled antibody technique (ELAT) and general cultural method (CM).


RESULTS
The isolation rate of anaerobes was rather high in pyothorax (88.9%), abdominal abscess (86.7%), appendicitis (83.9%) and cerebral abscess (all five cases were positive). In burn wounds, the rate was about 23.6% especially in deep burn wounds. Among 212 strains detected, the predominant anaerobes were as follows B. melaninogenicus (28.8%), B. fragilis (25%), F. nucleatum (7.5%), C. perfringens (7.5%), and peptostreptococcus (5.7%); most of them (87.8%) were non-sporing anaerobes. Mixed infection with aerobes accounted for 64%. The survey of rapid detection was conducted in B. fragilis, B. melaninogenicus, C. perfringens, F. nucleatum and peptostreptococcus. The detectable rate was higher in IFA (79.1%) and ELAT (80.9%) than in CM (37.3%).


CONCLUSIONS
IFA and ELAT might be rapid, specific, sensitive, and simple for detecting anaerobes.
Zhang
Y
Y
Institute of Burn Research, Southwestern Hospital, Third Military Medical College, Chongqing 400038.
Xiao
G
G
Qin
X
X
Li
A
A
D018784:Abdominal Abscess / Q000382:microbiology*; D001064:Appendicitis / Q000382:microbiology; D016866:Bacteroidaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D001442:Bacteroides Infections / Q000175:diagnosis / Q000382:microbiology; D001441:Bacteroides fragilis / Q000302:isolation & purification; D002056:Burns / Q000382:microbiology*; D016724:Empyema, Pleural / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D001443:Prevotella melaninogenica / Q000302:isolation & purification*