title: Expression of the stress-induced p8 mRNA is transiently activated after culture medium change.
abstract: We report here that the mere fact of changing culture medium for fresh medium induced in several cell lines the expression of stress-activated genes including protein kinases p38, JNK and ERK1/2 and the transcription factor C/EBPbeta. As a consequence, p8, a gene induced by stress in several tissues, was strongly up-regulated. Induction did not occur after change for cell-conditioned medium. Induction was however transient, with a peak at 60 min for p38, at 15-30 min for JNK and at 15 min for ERK1/2, at 2-3 hours for C/EBPbeta and at 4-6 hours for p8. Repression of the induction was due to the secretion of thermolabile molecule(s) that progressively conditioned the medium. As low as 25% of conditioned medium added to fresh culture medium was sufficient to abolish the stress response. Taken together, our data indicate that the renewal of culture medium induces a transient cellular stress that may be a source of artifacts in experiments performed shortly after a change of culture medium.
authors.affiliation: Centre de Recherche INSERM, EMI 0116, Marseille/France.
authors.forename: A
authors.initials: A
authors.lastname: Garcia-Montero
authors.forename: S
authors.initials: S
authors.lastname: Vasseur
authors.forename: G V
authors.initials: GV
authors.lastname: Mallo
authors.forename: P
authors.initials: P
authors.lastname: Soubeyran
authors.forename: J C
authors.initials: JC
authors.lastname: Dagorn
authors.forename: J L
authors.initials: JL
authors.lastname: Iovanna
mesh_terms: D016475:3T3 Cells; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects* / Q000502:physiology; D006133:Growth Substances / Q000235:genetics*; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009363:Neoplasm Proteins; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D017077:Culture Media, Conditioned; D004268:DNA-Binding Proteins; D006133:Growth Substances; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020929:Mitogen-Activated Protein Kinase Kinases; D020928:Mitogen-Activated Protein Kinases; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D048051:p38 Mitogen-Activated Protein Kinases; C083058:C-EPB transcription factor; C491643:NUPR1 protein, human; C491644:Nupr1 protein, mouse