Genomic structure of the human beta-PIX gene and its alteration in gastric cancer.
beta-PIX, a newly identified p21-activated kinase (PAK)-interacting exchange factors (PIX), encodes a guanine nucleotide exchange factor for Rho guanosine triphosphatases. Characterization of beta-PIX gene was performed using the BAC Library method. The beta-PIX gene has 17 exons and an A/T polymorphism at the 32nd base upstream of the intron/exon junction of exon 7. The frequencies of genotypes A/T, A/A and T/T were 23.6% (13/55), 72.7% (40/55) and 3.6% (2/55), respectively; these frequencies are in Hardy-Weinberg equilibrium. Two out of 14 informative tumors (14.3%) were shown to have lost their heterozygosity at this locus, but no mutations in the remaining alleles were detected. In addition, we examined the gene-expression profile in another set of 30 gastric samples, but no significant over-expression of either the beta-PIX gene or the alpha-PIX gene was found. Though the beta-PIX gene has been speculated to potentially have tumor-related biological characteristics, the findings of the present study suggest that the involvement of beta-PIX gene in human gastric carcinogenesis is minimal.
Li
Zhong you
Zy
First Department of Pathology, Hamamatsu University School of Medicine, 1-20-1, Handayama, Hamamatsu 431-3192, Japan.
Wang
You jie
Yj
Song
Jian ping
Jp
Kataoka
Hideki
H
Yoshii
Shigeto
S
Gao
Chang ming
Cm
Wang
Ya ping
Yp
Zhou
Jian nong
Jn
Ota
Satoshi
S
Tanaka
Masamitsu
M
Sugimura
Haruhiko
H
D000483:Alleles; D018797:Cell Cycle Proteins / Q000235:genetics*; D015894:Genome, Human; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D011110:Polymorphism, Genetic; D064067:Rho Guanine Nucleotide Exchange Factors; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*
C576606:ARHGEF7 protein, human; D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D064067:Rho Guanine Nucleotide Exchange Factors