The carboxyl terminus of the epsilon subunit of the chloroplast ATP synthase is exposed during illumination.
The epsilon subunit of the chloroplast ATP synthase is an inhibitor of activity of the enzyme. Recombinant forms of the epsilon subunit from spinach chloroplasts lacking the last 10, 32, or 45 amino acids were immobilized onto activated Sepharose. A polyclonal antiserum raised against the epsilon subunit was passed over these immobilized protein columns, and the purified antibodies which were not bound recognized the portions of the epsilon subunit missing from the recombinant form present on the column. The full polyclonal antiserum can strip the epsilon subunit from the ATP synthase in illuminated thylakoid membranes [Richter, M. L., and McCarty, R. E. (1987) J. Biol. Chem. 262, 15037-15040]. Exposure of illuminated thylakoid membranes to antibodies recognizing the last 32 amino acids of the epsilon subunit collapses the proton gradient and hinders ATP synthesis with similar efficiency as the full polyclonal preparation. These results indicate that antibodies against the last 32 amino acids of the epsilon subunit are capable of stripping the subunit from the ATP synthase in illuminated membranes. Neither of these effects was seen when the membranes were exposed to the antibodies in the dark. This is direct evidence that the chloroplast ATP synthase undergoes a conformational shift during its activation by the electrochemical proton gradient which specifically alters the conformation of the carboxyl-terminal domain of the epsilon subunit from protected to solvent-exposed. The relation between this shift and activation of the enzyme by the electrochemical proton gradient is discussed.
Johnson
Eric A
EA
Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218-2685, USA.
McCarty
Richard E
RE
D000906:Antibodies / Q000032:analysis / Q000302:isolation & purification; D025222:Chloroplast Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation; D000939:Epitopes / Q000032:analysis; D008027:Light; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000276:immunology; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000276:immunology; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea; D020524:Thylakoids / Q000201:enzymology; D000097795:ATPase Inhibitory Protein
D000906:Antibodies; D025222:Chloroplast Proton-Translocating ATPases; D000939:Epitopes; D010446:Peptide Fragments; D011506:Proteins; D000097795:ATPase Inhibitory Protein