Inability to clonally expand fetal progenitors from maternal blood.
OBJECTIVE
To confirm the recent report of the culture of single clones of fetal progenitor cells from maternal blood.


METHODS
Hemopoietic progenitor cells were cultured from 16 blood samples obtained from women pregnant with a male singleton fetus. Single colonies were isolated by micro-manipulation and examined by multiplex real-time PCR.


RESULTS
Of a total of 1,674 colonies examined, 1,648 were identified as being maternal. No colonies were detected of fetal origin.


CONCLUSIONS
The clonal expansion of single fetal colonies from the maternal circulation is not feasible by current methods.
Zimmermann
Bernhard
B
Department of Obstetrics and Gynaecology, University of Basel, Switzerland.
Holzgreve
Wolfgang
W
Zhong
Xiao Yan
XY
Hahn
Sinhue
S
D002452:Cell Count; D018929:Cell Culture Techniques; D002469:Cell Separation; D002999:Clone Cells / Q000166:cytology*; D004268:DNA-Binding Proteins / Q000235:genetics; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005865:Gestational Age; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D008297:Male; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases