title: Comparison of three dsDNA-ELISAs with regard to their efficiency in the diagnosis of systemic lupus erythematosus.
abstract: We compared the performance of three commercially available anti-double-stranded DNA antibody ELISA kits with respect to their precision, accuracy, linearity, and the detection limit. We tested six sets of patients (lupus erythematosus, scleroderma, rheumatoid arthritis, psoriatic arthritis, hepatitis C, malignancies) to assess specificity, sensitivity, and diagnostic efficiency of the three assays. The diagnostic efficiency of the Crithidia luciliae immunofluorescence test was analyzed as a reference. The sensitivity of the evaluated enzyme immunoassays ranged from 55.6 to 66.7%, the specificity from 98.4 to 100%, and the diagnostic efficiency from 90.2-92.7%. All ELISAs were superior to the immunofluorescence test, both with respect to sensitivity, specificity, and diagnostic efficiency. The commercial kits tested were comparable with respect to their performance characteristics.
authors.affiliation: Institute of Clinical Chemistry, University of Cologne, Köln, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Linnemann
authors.forename: Christoph
authors.initials: C
authors.lastname: Geisen
authors.forename: Sabine
authors.initials: S
authors.lastname: Menn
authors.forename: Christian
authors.initials: C
authors.lastname: Herzberg
authors.forename: Robert
authors.initials: R
authors.lastname: Dinser
authors.forename: Klaus
authors.initials: K
authors.lastname: Wielckens
mesh_terms: D000974:Antibodies, Antinuclear; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000235:genetics; D002138:Calibration; D016022:Case-Control Studies; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000235:genetics; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000974:Antibodies, Antinuclear; D011933:Reagent Kits, Diagnostic; D004247:DNA