Membrane anchors for vacuolar targeting: application in plant bioreactors.
Transgenic plants are attractive expression systems for producing recombinant proteins. Plant cells compartmentalize and store metabolites and proteins in vacuoles, but foreign proteins need to be targeted to the correct compartments if they are to accumulate in a stable fashion. Here we present a general strategy in which unique transmembrane and cytoplasmic tail sequences are used as anchors for delivering recombinant proteins via distinct vesicular transport pathways to specific vacuolar compartments where stable accumulation can occur.
Jiang
Liwen
L
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. ljiang@cuhk.edu.hk
Sun
Samuel S M
SS
D019149:Bioreactors; D008565:Membrane Proteins / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D010940:Plant Proteins; D021382:Protein Sorting Signals; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D033921:Vesicular Transport Proteins; C078044:alpha-TIP protein, Phaseolus vulgaris