Expression of telomerase genes as potential marker of neoplastic changes.
We conducted a quantitative analysis of TERT, TP1 and hTR mRNA expression in various types of endometrial hyperplasia in perimenopausal women, taking advantage of the real-time PCR assay. All women underwent hysterectomy for gynecological reasons. Endometrial dating was determined from the patomorphology of the endometrium and classified into endometrial hyperplasia: simplex, complex and atypica. Our data suggest that only hTR was observed in each normal and hyperplastic endometrium specimens, suggesting that this factor constitutively expressed in endometrium. The results obtained indicate that the expression activity of the TERT subunit changes but not significantly, depending on the stage of development of the hyperplasia.
Mazurek
U
U
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Katowice. umazurek@farmant.slam.katowice.pl
Witek
A
A
Olejek
A
A
Paul
M
M
Skałba
P
P
Wilczok
T
T
D000328:Adult; D000368:Aged; D017398:Alternative Splicing; D014408:Biomarkers, Tumor / Q000235:genetics; D002471:Cell Transformation, Neoplastic; D002979:Climacteric; D004714:Endometrial Hyperplasia / Q000235:genetics* / Q000473:pathology; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D019098:Telomerase / Q000235:genetics*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D019098:Telomerase