title: Simple method for reduction of autofluorescence in fluorescence microscopy.
abstract: Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.
authors.affiliation: Department of Chemistry, University of Bremen, Bremen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Neumann
authors.forename: Detlef
authors.initials: D
authors.lastname: Gabel
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000737:chemistry; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000592:standards*; D011786:Quality Control; D016610:Tissue Embedding; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein