Cellular localization of orexin receptors in human adrenal gland, adrenocortical adenomas and pheochromocytomas.
Orexin-A and -B are hypothalamic peptides derived from a precursor called prepro-orexin and related with the regulation of the energy balance and arousal. They act on G protein receptors named orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). In the present study, we used immunohistochemical techniques to detect the distribution of OXR in normal human adrenal gland and adrenal tumours (adrenocortical adenomas and pheochromocytomas). OX1R was expressed in the cortex of the normal human adrenal gland (glomerulosa, fasciculata and reticular zones) and OX2R was located in the medulla (epinephrine and norepinephrine cells). By the double immunofluorescence techniques, we demonstrated that virtually all medullar cells (epinephrine and norepinephrine cells) expressed OX2R. As was expected, according to the results obtained in normal tissues, cortical tumours (adrenocortical adenomas) were positive for OX1R but not for OX2R and conversely, medullar tumours (pheochromocytomas) expressed only OX2R.
Blanco
Montserrat
M
Department of Morphological Sciences, Faculty of Medicine, Clinical University Hospital, University of Santiago de Compostela, c/ San Francisco s/n, E-15782, Santiago de Compostela, Spain.
García-Caballero
Tomás
T
Fraga
Máximo
M
Gallego
Rosalía
R
Cuevas
Juan
J
Forteza
Jerónimo
J
Beiras
Andrés
A
Diéguez
Carlos
C
D000293:Adolescent; D000302:Adrenal Cortex / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism; D018246:Adrenocortical Adenoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D064446:Orexin Receptors; D010673:Pheochromocytoma / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000096:biosynthesis / Q000378:metabolism*; D014018:Tissue Distribution / Q000502:physiology
C578626:HCRTR2 protein, human; D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide