title: Binding inhibition of type 1 fimbriae to human granulocytes: a flow cytometric inhibition assay using trivalent cluster mannosides.
abstract: The binding of type 1 fimbriae from Escherichia coli to vital human neutrophilic granulocytes was inhibited by synthetic trivalent cluster mannosides. Binding of type 1 fimbriae was measured in a flow cytometric assay. Based on the molarity of mannosyl residues, the clusters exceed the inhibitory potency of methyl alpha-D-mannoside by a factor of more than 1,000 and the inhibitory potency of p-nitrophenyl alpha-D-mannoside by a factor of more than 10. The inhibition studies indicate that the trivalent cluster mannosides are very potent inhibitors of the binding of type 1 fimbriae to human neutrophilic granulocytes. Based on their defined structure, cluster mannosides are well suited for characterizing the molecular interactions of mannose-sensitive fimbriae with their cell membrane receptors.
authors.affiliation: University Hospital Eppendorf, Department of Clinical Chemistry, Hamburg, Germany. ahorst@med.mcgill.ca
authors.forename: A K
authors.initials: AK
authors.lastname: Horst
authors.forename: S
authors.initials: S
authors.lastname: Kötter
authors.forename: T K
authors.initials: TK
authors.lastname: Lindhorst
authors.forename: A
authors.initials: A
authors.lastname: Ludwig
authors.forename: E
authors.initials: E
authors.lastname: Brandt
authors.forename: C
authors.initials: C
authors.lastname: Wagener
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008362:Mannosides / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009504:Neutrophils / Q000378:metabolism
chemical_list: D008362:Mannosides