title: Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology.
abstract: A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.
authors.affiliation: Children's Memorial Institute for Education and Research, Northwestern University, Chicago, Illinois 60614, USA. yzhao@northwestern.edu
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.forename: Johann W
authors.initials: JW
authors.lastname: Miller
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Chen
authors.forename: Eric G
authors.initials: EG
authors.lastname: Bremer
authors.forename: William
authors.initials: W
authors.lastname: Kabat
authors.forename: Ram
authors.initials: R
authors.lastname: Yogev
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D019914:Taq Polymerase