title: The effects of folic acid application on IL-1beta levels of human gingival fibroblasts stimulated by phenytoin and TNFalpha in vitro: a preliminary study.
abstract: Folic acid (FA), that is required for the integrity of gingival tissues, was found to decrease in patients using phenytoin (PHT). Interleukin-1 beta (IL-1beta) was reported to enhance the extracellular matrix synthesis in fibroblasts. The purpose of this study was to assess the role of FA supplementation on PHT-induced overgrowth by investigating its effect on IL-1beta production of human gingival fibroblasts induced by tumor necrosis factor alfa (TNFalpha) in cell culture. PHT (20 microg/ml), FA (20 or 40 ng/ml) + PHT, PHT + TNF (10 ng/ml), FA (20 or 40 ng/ml) + PHT + TNF, or only culture medium (control) was added to 24-well plates containing fibroblasts. After an incubation period of 72 h, culture medium and cells were harvested separately. Then, IL-1beta levels in cell lysate were measured using enzyme-linked immunosorbent assay. The cellular IL-1beta level in the PHT group was 1 pg/ml. In PHT + 20 or 40 ng/ml FA-added cultures, the results obtained respectively were 0.8 and 0.7 pg/ml, whereas the control group value was 0.7 pg/ml. IL-1beta level was 4 pg/ml in the cultures that PHT and TNFalpha were applied simultaneously (P < 0.05). When PHT and either 20 or 40 ng/ml FA were simultaneously added into TNFalpha-induced cultures, the IL-1beta levels were 1.8 and 1.3 pg/ml, respectively. IL-1beta level in gingival tissues might play a role in PHT-induced overgrowth by increasing in the gingival tissues, and FA application might play a role in decreasing gingival tissues. However, further studies are needed for a more complete understanding of PHT-induced gingival overgrowth at the cellular level.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. doganaltan@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Doğan
authors.forename: Y
authors.initials: Y
authors.lastname: Tunca
authors.forename: A
authors.initials: A
authors.lastname: Ozdemir
authors.forename: A
authors.initials: A
authors.lastname: Sengül
authors.forename: N
authors.initials: N
authors.lastname: Imirzalioğlu
mesh_terms: D000927:Anticonvulsants / Q000494:pharmacology*; D018929:Cell Culture Techniques; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005492:Folic Acid / Q000494:pharmacology*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019214:Gingival Overgrowth / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D010672:Phenytoin / Q000494:pharmacology*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D000927:Anticonvulsants; D003470:Culture Media; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D010672:Phenytoin; D005492:Folic Acid