title: Transport of N-acetyl-D-mannosamine and N-acetyl-D-glucosamine in Escherichia coli K1: effect on capsular polysialic acid production.
abstract: N-Acetyl-D-mannosamine (ManNAc) and N-acetyl-D-glucosamine (GlcNAc) are the essential precursors of N-acetylneuraminic acid (NeuAc), the specific monomer of polysialic acid (PA), a bacterial pathogenic determinant. Escherichia coli K1 uses both amino sugars as carbon sources and uptake takes place through the mannose phosphotransferase system transporter, a phosphoenolpyruvate-dependent phosphotransferase system that shows a broad range of specificity. Glucose, mannose, fructose, and glucosamine strongly inhibited the transport of these amino-acetylated sugars and GlcNAc and ManNAc strongly affected ManNAc and GlcNAc uptake, respectively. The ManNAc and the GlcNAc phosphorylation that occurs during uptake affected NeuAc synthesis in vitro. These findings account for the low in vivo PA production observed when E. coli K1 uses ManNAc or GlcNAc as a carbon source for growth.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24007 León, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Revilla-Nuin
authors.forename: Angel
authors.initials: A
authors.lastname: Reglero
authors.forename: Honorina
authors.initials: H
authors.lastname: Martínez-Blanco
authors.forename: Ignacio G
authors.initials: IG
authors.lastname: Bravo
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Ferrero
authors.forename: Leandro B
authors.initials: LB
authors.lastname: Rodríguez-Aparicio
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D001692:Biological Transport; D050260:Carbohydrate Metabolism; D002455:Cell Division; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D006595:Hexosamines / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010770:Phosphotransferases / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D012794:Sialic Acids / Q000096:biosynthesis*; D013696:Temperature; D013997:Time Factors
chemical_list: D006595:Hexosamines; D011135:Polysaccharides, Bacterial; D012794:Sialic Acids; C021319:polysialic acid; D010770:Phosphotransferases; D000117:Acetylglucosamine; C002022:N-acetylmannosamine