Isolation of an esterase-producing Trichosporon brassicae and its catalytic performance in kinetic resolution of ketoprofen.
A yeast strain CGMCC 0574, identified as Trichosporon brassicae, was selected from 92 strains for its high (S) selectivity in the hydrolysis of ketoprofen ethyl ester. The effective strains of the microorganisms were isolated from soil samples with the ester as the sole carbon source. The ethyl ester proved to be the best substrate for resolution of ketoprofen among several ketoprofen esters examined. The resting cells of CGMCC 0574 could catalyze the hydrolysis of ketoprofen ethyl ester with an enantiomeric ratio of 44.9, giving (S)-ketoprofen an enantiomeric excess of 91.5% at 42% conversion.
Shen
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.
Xu
J H
JH
Gong
P F
PF
Wu
H Y
HY
Liu
Y Y
YY
D004950:Esterases / Q000096:biosynthesis; D004952:Esters / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000096:biosynthesis*; D006868:Hydrolysis; D007660:Ketoprofen / Q000493:pharmacokinetics*; D014250:Trichosporon / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism
D004952:Esters; D007660:Ketoprofen; D006867:Hydrolases; D004950:Esterases