Monitoring of basophil activation using CD63 and CCR3 in allergy to muscle relaxant drugs.
Allergic or pseudoallergic reactions that occur during anesthesia have been increasing for the last few years. To date, the diagnosis of allergy to muscle relaxants remains difficult. In this respect, we developed a flow cytometric method for the study of drug-induced basophil degranulation using CD63 and CCR3. Fifty patients who developed clinical features evocative of allergic reactions immediately after induction of anesthesia were included and classified into two groups. Group 1 (n = 39) comprised true allergic patients, who developed typical signs of shock associated to positive skin testing. Group 2 (n = 11) consisted of patients whose clinical history was not typical and skin testing was negative or nonconclusive. Seventeen control subjects were also studied in this report. We compared data from flow cytometry to skin tests, specific IgE, and histamine release results. Flow cytometry showed a sensitivity of 54%, while that of specific IgE was similar, at 62%. Interestingly, when considering the sensitivity of IgE + CD63 for diagnosis, we reached a sensitivity value of 80%. Of 15 negative results for specific IgE, we found 7 positive CD63 tests, while histamine release gave positive results in only 2 cases. Furthermore, the CD63 protocol showed good specificity (100%). We conclude that our flow cytometry protocol is a promising tool in allergy diagnosis since it is specific and complementary to specific IgE detection.
Monneret
Guillaume
G
Immunology Laboratory, Lyon-Sud University Hospital, Pierre-Bénite, France.
Benoit
Yves
Y
Debard
Anne Lise
AL
Gutowski
Marie C
MC
Topenot
Ingrid
I
Bienvenu
Jacques
J
D000768:Anesthesia, General / Q000009:adverse effects*; D015703:Antigens, CD / Q000276:immunology*; D001491:Basophils / Q000276:immunology*; D015550:Cell Degranulation / Q000276:immunology*; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000276:immunology*; D012680:Sensitivity and Specificity; D060149:Tetraspanin 30
D015703:Antigens, CD; C515350:CCR3 protein, human; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D060149:Tetraspanin 30