title: Acute effects of ozone exposure on lung function in mice sensitized to ovalbumin.
abstract: Pulmonary responses to ozone exposure (1.0 ppm) were investigated in mice sensitized to ovalbumin compared with control mice receiving saline. Pulmonary function parameters were measured by pneumotachography. Arterial blood gases and the concentrations of soluble intercellular adhesion molecule-1 (sICAM-1) and tumor necrosis factor-alpha (TNF-alpha) in bronchoalveolar lavage fluid were analyzed. Ozone exposure, when compared with filtered air exposure, caused significantly larger decreases in dynamic compliance (P<0.05) and minute ventilation (P<0.05) in ovalbumin-sensitized mice but not in control mice. Moreover, the decrease in minute ventilation in response to ozone exposure was significantly greater (P<0.01) in ovalbumin-sensitized mice than in control mice. Ozone exposure caused a significant decrease in PaO2 in ovalbumin-sensitized mice but not in control mice. PaO2 after ozone exposure tended to be smaller in ovalbumin-sensitized mice than in control mice. The concentration of sICAM-1 in bronchoalveolar lavage fluid increased in ovalbumin-sensitized mice, but effects of ozone exposure were not observed. These results indicated that sensitization of the immune system to ovalbumin might be a risk factor which aggravates the effects of ozone exposure on the respiratory system.
authors.affiliation: Department of Public Health, Chiba University School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. sabu-maru-yamauchi@nifty.com
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shima
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kuwaki
authors.forename: Michiko
authors.initials: M
authors.lastname: Ando
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Ohmichi
authors.forename: Yasuichiro
authors.initials: Y
authors.lastname: Fukuda
authors.forename: Motoaki
authors.initials: M
authors.lastname: Adachi
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D008168:Lung / Q000187:drug effects*; D008297:Male; D016210:Methacholine Chloride; D051379:Mice; D008810:Mice, Inbred C57BL; D018721:Muscarinic Agonists; D010047:Ovalbumin / Q000276:immunology*; D010083:Oxidants, Photochemical / Q000008:administration & dosage / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D012129:Respiratory Function Tests; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018721:Muscarinic Agonists; D010083:Oxidants, Photochemical; D014409:Tumor Necrosis Factor-alpha; D016210:Methacholine Chloride; D010126:Ozone; D010047:Ovalbumin