title: Proteomics and immunomapping of reactive lymph-node and lymphoma.
abstract: In the present study we show that two-dimensional (2-D) maps together with immuno-detection allow the precise identification of important leukocyte differentiation and tumor markers (e.g., CD3 and CD5), and important cell cycle regulatory molecules such as cyclin dependent kinases, notably CDK6. In addition, the comparative evaluation of molecular expression (e.g., CD5) in maps developed with normal and lymphoma samples can provide reproducible and precise information regarding the molecular expression in different cell populations. Accordingly, we could detect a much increased level of expression of CD5 in mantle cell lymphoma, up to ten times higher than in the control. In addition, CD5 in tumor tissues seems to be microheterogeneous as compared to normal samples.
authors.affiliation: Department of Agricultural and Industrial Biotechnologies, University of Verona, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Antonucci
authors.forename: Marco
authors.initials: M
authors.lastname: Chilosi
authors.forename: Maria
authors.initials: M
authors.lastname: Santacatterina
authors.forename: Ben
authors.initials: B
authors.lastname: Herbert
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008198:Lymph Nodes / Q000737:chemistry; D008223:Lymphoma / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome