title: The diagnostic value of kappa/lambda ratios determined by flow cytometric analysis of biopsy specimens in B-cell lymphoma.
abstract: Flow cytometry (FC) is widely utilized in the diagnosis of lymphoma and the light chain ratio (LCR) is especially useful in the diagnosis of B-cell malignancy. In this study we analysed, retrospectively, the predictive value of the LCR in the diagnosis of B-cell lymphoma in 105 consecutive patients with persistent lymph node enlargement or extranodal masses who underwent biopsy. We used a receiver-operating characteristic curve to establish a LCR threshold value of 2.0. The specificity, sensitivity, positive and negative predictive values were 92.3%, 73.1%, 90% and 77%, respectively. We concluded that determination of LCR is a useful adjunct to pathological diagnosis.
authors.affiliation: Department of Haematology, International Medical Center of Japan, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Chizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Kanda
authors.forename: Y
authors.initials: Y
authors.lastname: Nannya
authors.forename: K
authors.initials: K
authors.lastname: Oshima
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: R
authors.initials: R
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Suguro
authors.forename: T
authors.initials: T
authors.lastname: Hamaki
authors.forename: T
authors.initials: T
authors.lastname: Matsuyama
authors.forename: N
authors.initials: N
authors.lastname: Takezako
authors.forename: A
authors.initials: A
authors.lastname: Miwa
authors.forename: A
authors.initials: A
authors.lastname: Togawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis* / Q000145:classification; D007145:Immunoglobulin kappa-Chains / Q000032:analysis; D007146:Immunoglobulin lambda-Chains / Q000032:analysis; D016393:Lymphoma, B-Cell / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains