title: Lymphoid neoplasms in HIV-positive individuals in India.
abstract: The HIV epidemic in the Asian subcontinent has a significant impact on India. Patients with AIDS have an increased risk of developing non-Hodgkin lymphoma (NHL). In this study, we have investigated the pattern of distribution of lymphoid neoplasms and also studied the Epstein-Barr virus (EBV)-association and p53 expression in 35 HIV-positive patients from India. The biopsy samples were studied for histology and for expression of CD20, CD3, CD15, CD30, light chains, CD138, bcl-6, epithelial membrane antigen, EBV-latent membrane protein-1, and p53 protein. In situ hybridization was performed with digoxigenin-labeled anti-sense EBV-encoded nuclear RNA-1 (EBER-1) probe. Polymerase chain reaction (PCR) was performed on DNA extracted from paraffin sections for EBV-subtype analysis. The 35 cases included 7 cases of Hodgkin disease (HD), 4 cases of plasmacytoma (PL), and 24 cases of NHL. Among the cases of NHL, 3 were Burkitt lymphoma (BL), 4 were diffuse large B-cell lymphoma (DLBL) of centroblastic type (CBL), 10 were DLBL of immunoblastic type (IBL), 4 were high-grade B-cell lymphoma (unspecified) and the rest were other subtypes. EBV-association was noted in all cases of HD, 2 of 3 BL, and 3 of 10 IBL. PCR analysis of the EBNA-3C gene revealed amplimers corresponding to type A. A p53 protein overexpression was noted in 6 of 10 IBLs, 1 of 3 BLs, 2 of 3 CBLs, and 5 of 7 cases of HD. This is the first reported study of lymphoid malignancies in HIV-positive individuals from India.
authors.affiliation: Lymphoma Registry, Department of Pathology, Tata Memorial Hospital, Mumbai, India.
authors.forename: Beamon
authors.initials: B
authors.lastname: Agarwal
authors.forename: Uma
authors.initials: U
authors.lastname: Ramanathan
authors.forename: Nilesh
authors.initials: N
authors.lastname: Lokeshwas
authors.forename: Reena
authors.initials: R
authors.lastname: Nair
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Gopal
authors.forename: Kishor
authors.initials: K
authors.lastname: Bhatia
authors.forename: Kikkeri N
authors.initials: KN
authors.lastname: Naresh
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis; D002051:Burkitt Lymphoma / Q000145:classification / Q000150:complications; D005260:Female; D006679:HIV Seropositivity / Q000150:complications* / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D004854:Herpesvirus 4, Human; D006689:Hodgkin Disease / Q000145:classification / Q000150:complications; D006801:Humans; D007194:India / Q000453:epidemiology; D008223:Lymphoma / Q000145:classification / Q000150:complications* / Q000276:immunology / Q000821:virology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000145:classification / Q000150:complications; D016400:Lymphoma, Large-Cell, Immunoblastic / Q000145:classification / Q000150:complications; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000150:complications; D008297:Male; D010954:Plasmacytoma / Q000145:classification / Q000150:complications; D012367:RNA, Viral / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D014763:Viral Matrix Proteins / Q000096:biosynthesis
chemical_list: D015703:Antigens, CD; C037662:EBV-associated membrane antigen, Epstein-Barr virus; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral; D016159:Tumor Suppressor Protein p53; D014763:Viral Matrix Proteins