Differential display of vincristine-resistance-related genes in gastric cancer SGC7901 cell.
OBJECTIVE
To isolate and clone the vincristine-resistance-related genes in gastric cancer SGC7901 cell line and to clarify the multidrug-resistant molecular mechanism of gastric cancer cells.


METHODS
The modified differential-display polymerase chain reaction (DD-PCR) was used to examine differences in the mRNA composition of Vincristine-resistant gastric cancer SGC 7901 cells (SGC7901/VCR), induced by vincristine sulfate versus SGC7901cells. The differentially expressed cDNA fragments were confirmed by reverse Northern analysis, sequencing, BLAST analysis and Northern bolt analysis.


RESULTS
The DD-PCR identified that 54 cDNA fragments were preferentially expressed in SGC 7901/VCR cells. When these cDNA fragments were analyzed by reverse Northern blot, twenty were reproducibly expressed at high level in SGC7901/VCR. Sequencing and BLAST analysis revealed that seven of the genes were known genes:ADP-ribosylation factor 4, Cytochrome oxidase subunit II, Ss-A/Ro ribonucleoprtein autoantigen 60kd subunit,ribosomal protein S13, galaectin-8 gene, oligophrenin 1 mRNA, ribosomal protein L23 mRNA; thirteen of the genes were unknown genes. The length and abundance of the four unknown genes were further confirmed by Northern blot analysis.


CONCLUSIONS
The twenty differential known and unknown genes may be related to the vincristine-resistant mechanism in human gastric cancer SGC7901 cell line.
Wang
Xin
X
Institute of Digestive disease, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, Shaanxi Province, China.
Lan
Mei
M
Shi
Yong-Quan
YQ
Lu
Ju
J
Zhong
Yue-Xia
YX
Wu
Han-Ping
HP
Zai
Hui-Hong
HH
Ding
Jie
J
Wu
Kai-Cun
KC
Pan
Bo-Rong
BR
Jin
Jian-Ping
JP
Fan
Dai-Ming
DM
D020727:ADP-Ribosylation Factors / Q000235:genetics; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001324:Autoantigens / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D019008:Drug Resistance, Neoplasm; D003576:Electron Transport Complex IV / Q000235:genetics; D029968:Escherichia coli Proteins; D020690:GTPase-Activating Proteins; D037161:Galectins; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D037102:Lectins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics; D020733:RNA, Small Cytoplasmic; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D013274:Stomach Neoplasms; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014750:Vincristine / Q000494:pharmacology*; D000099207:SS-A Antigen
D020727:ADP-Ribosylation Factors; D000972:Antineoplastic Agents, Phytogenic; D001324:Autoantigens; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D003576:Electron Transport Complex IV; D029968:Escherichia coli Proteins; D020690:GTPase-Activating Proteins; D037161:Galectins; D037102:Lectins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D014750:Vincristine; D000099207:SS-A Antigen; C091991:LGALS8 protein, human; C111952:OPHN1 protein, human; C116006:RO60 protein, human; C043816:ribosomal protein L23, E coli