title: Expression and regulation of the nuclear receptor RORalpha in human vascular cells.
abstract: Retinoic acid receptor-related orphan receptor alpha (RORalpha) is a member of the nuclear receptor superfamily. Using RT-PCR, RORalpha mRNA was identified in human aortic smooth muscle cells (hASMC), endothelial cells (EC), as well as in human mammary arteries and atherosclerotic plaques. We found a predominant expression of RORalpha1 in hASMC, and RORalpha4 in EC. RORalpha2 and RORalpha3 were not detected. In arteries, RORalpha4 was predominant compared with RORalpha1. In atherosclerotic plaques, RORalpha expression was significantly decreased. In hASMC stimulated with cytokines, RORalpha expression was increased by 2.5-fold. RORalpha mRNA was also significantly increased (approximately 2-fold) in hASMC and EC cultured under hypoxia.
authors.affiliation: INSERM U541, Hôpital Lariboisière, 75475 Paris Cedex 10, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Besnard
authors.forename: Christophe
authors.initials: C
authors.lastname: Heymes
authors.forename: Régine
authors.initials: R
authors.lastname: Merval
authors.forename: Marianne
authors.initials: M
authors.lastname: Rodriguez
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Galizzi
authors.forename: Jean A
authors.initials: JA
authors.lastname: Boutin
authors.forename: Jean
authors.initials: J
authors.lastname: Mariani
authors.forename: Alain
authors.initials: A
authors.lastname: Tedgui
mesh_terms: D001011:Aorta; D001161:Arteriosclerosis / Q000235:genetics / Q000378:metabolism; D016893:Carotid Stenosis / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008323:Mammary Arteries / Q000166:cytology / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D010100:Oxygen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; D012333:RNA, Messenger; C539738:RORA protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D015534:Trans-Activators; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen