Comparative studies of various run buffers for chiral capillary electrophoresis using chiral crown ether as a chiral selector.
In the capillary electrophoretic separation of primary amine enantiomers using (+)-(18-crown-6)-tetracarboxylic acid (18C6H4) as a chiral selector, the presence of run buffer constituents such as tris(hydroxymethyl)aminomethane (Tris) or Na+ competing with analytes for 18C6H4, diminishes the effectiveness of 18C6H4. In order to determine appropriate buffer systems for 18C6H4, various run buffer cationic components including Tris, 1,3-bis[tris(hydroxymethyl)methylamino]propane, bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane, triethanolamine, tetramethylammonium, and Na+ were compared. Quantitative studies of the effects of the competitive constituents were carried out by measuring the electrophoretic mobilities of histidine as a function of the 18C6H4 concentration. We also derived a simple equation to estimate the optimal chiral selector concentration for a maximum mobility difference in the presence of a competitive inhibitor.
Jang
J
J
Division of Chemistry and Molecular Engineering, School of Chemistry, Seoul National University, Korea.
Cho
S I
SI
Chung
D S
DS
D000588:Amines / Q000737:chemistry*; D002021:Buffers; D002412:Cations; D019075:Electrophoresis, Capillary / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry; D013237:Stereoisomerism
D000588:Amines; D002021:Buffers; D002412:Cations; D004988:Ethers, Cyclic; D007202:Indicators and Reagents; D012964:Sodium