Gas-phase reactions of charged phenyl radicals with neutral biomolecules evaporated by laser-induced acoustic desorption.
A generally applicable method for the study of phenyl radicals' reactions with neutral biomolecules in the gas phase is demonstrated. Neutral biomolecules were evaporated into a Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR) by means of laser-induced acoustic desorption (LIAD) and subsequently reacted with trapped charged phenyl radicals. The structural integrity of the evaporated alanylalanine molecules was verified by reaction with dichlorophosphenium ions. Examination of the reactions of charged phenyl radicals with alanylalanine and thymidine evaporated via LIAD revealed hydrogen atom abstraction for both alanylalanine and thymidine as well as an addition/elimination product for the reaction with thymidine. These reactions are consistent with the results obtained by others in solution. Further, a previously unstudied reaction of the nucleotide of thymine (T1) with charged phenyl radical was found to yield analogous products as the reaction with thymidine.
Petzold
Christopher J
CJ
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393, USA.
Ramírez-Arizmendi
Luis E
LE
Heidbrink
Jenny L
JL
Pérez
James
J
Kenttämaa
Hilkka I
HI
D017357:Cyclotrons; D004151:Dipeptides / Q000737:chemistry; D005583:Fourier Analysis; D005609:Free Radicals / Q000737:chemistry*; D007202:Indicators and Reagents; D007834:Lasers; D013058:Mass Spectrometry; D010455:Peptides / Q000737:chemistry*; D013936:Thymidine / Q000737:chemistry
D004151:Dipeptides; D005609:Free Radicals; D007202:Indicators and Reagents; D010455:Peptides; C002956:alanylalanine; D013936:Thymidine