title: Isolation and expression of a novel neuron-specific onecut homeobox gene in zebrafish.
abstract: A complete cDNA of a novel zebrafish gene named onecut has been isolated; this gene encodes a protein of 446 amino acids with a Cut domain (73 amino acid residues) and a homeodomain. The Cut domain of zebrafish Onecut is highly similar to those in mammalian hepatocyte nuclear factor-6 and Drosophila Onecut, sharing 90 and 88% amino acid identity, respectively. The expression of zebrafish onecut is restricted to neuronal cells, being first detected in trigeminal ganglia neurons at the end of gastrulation. By the 1-somite stage, onecut expression has begun in primary neurons of the lateral stripes in the neural plate, and appeared in neuronal cells of the medial stripes at the 2-somite stage. By the 4-somite stage, onecut expression expanded to the intermediate stripes and to subsets of neuronal cells in the midbrain and hindbrain. Subsequently, onecut expression intensified in the lateral region of midbrain and hindbrain, yet no onecut-positive cells were seen in the telencephalon. By 24hpf, onecut transcripts remained abundant in the spinal cord but were no longer detectable in differentiated Rohon-Beard sensory neurons. The expression of onecut was greatly increased in the neural mutant mindbomb, while being decreased in narrowminded.
authors.affiliation: Department of Genetic Engineering, Kyungpook National University, Daegu 702-701, South Korea.
authors.forename: Sung-Kook
authors.initials: SK
authors.lastname: Hong
authors.forename: Cheol-Hee
authors.initials: CH
authors.lastname: Kim
authors.forename: Kyeong-Won
authors.initials: KW
authors.lastname: Yoo
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Tetsuhiro
authors.initials: T
authors.lastname: Kudoh
authors.forename: Igor B
authors.initials: IB
authors.lastname: Dawid
authors.forename: Tae-Lin
authors.initials: TL
authors.lastname: Huh
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics*; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D029961:Zebrafish Proteins; C418486:onecut protein, Drosophila; C499617:onecut1 protein, zebrafish; C461685:onecutl protein, zebrafish