Molecular characterisation and chromosomal mapping of transcripts having tissue-specific expression in the malaria mosquito Anopheles gambiae: possible involvement in visual or olfactory processes.
We have compared the transcriptional activity of heads, antennae + palps, and carcasses in the mosquito Anopheles gambiae by means of differential display PCR (DD-PCR). Three transcripts specifically or preferentially expressed in the heads and in the antennae + palps have been selected. All are very similar to genes related to visual and olfactory mechanisms of several different organisms. They have been named Ag arrestin, Ag rLDL, and Ag dynamin. The potential of the DD-PCR technique in identifying genes involved in mosquito behaviour and the usefulness of the molecular characterisation of these transcripts are discussed.
Ricci
Irene
I
Istituto di Parassitologia, Università La Sapienza, Rome, Italy
Santolamazza
Federica
F
Costantini
Carlo
C
Favia
Guido
G
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D019393:Arrestin / Q000235:genetics / Q000502:physiology; D002874:Chromosome Mapping; D034281:Dynamins; D005136:Eye Proteins / Q000235:genetics*; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000502:physiology; D017344:Genes, Insect; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction / Q000379:methods; D011973:Receptors, LDL / Q000235:genetics / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D012903:Smell; D014158:Transcription, Genetic; D014785:Vision, Ocular
D019393:Arrestin; D005136:Eye Proteins; D019476:Insect Proteins; D011973:Receptors, LDL; C464401:arrestin, Anopheles gambiae; D020558:GTP Phosphohydrolases; C464402:dynamin, Anopheles gambiae; D034281:Dynamins