Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays.
PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3' end.
Nielsen
H B
HB
Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark. hbjorn@cbs.dtu.dk
Knudsen
S
S
D000818:Animals; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D030541:Databases, Genetic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data*; D016133:Polymerase Chain Reaction; D012984:Software
D017931:DNA Primers