Experimentally determined sequence requirement of ACGT-containing abscisic acid response element.
The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.
Hattori
Tsukaho
T
Center for Molecular Biology and Genetics, Mie University, Tsu, 514-8507 Japan. hattori@gene.mie-u.ac.jp
Totsuka
Masako
M
Hobo
Tokunori
T
Kagaya
Yasuaki
Y
Yamamoto-Toyoda
Akiko
A
D000040:Abscisic Acid / Q000494:pharmacology*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D051790:G-Box Binding Factors; D005786:Gene Expression Regulation / Q000187:drug effects; D005966:Glucuronidase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; C094468:Osem protein, Oryza sativa; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D000040:Abscisic Acid; D005966:Glucuronidase