title: Custom DNA-binding proteins come of age: polydactyl zinc-finger proteins.
abstract: Artificial transcription factors based on modified zinc-finger DNA-binding domains have been shown to activate or repress the transcription of endogenous genes in multiple organisms. Advances in both the construction of novel zinc-finger proteins and our understanding of the characteristics of a productive regulatory site have fueled these achievements.
authors.affiliation: The Skaggs Institute for Chemical Biology, Scripps Research Institute, BCC-550, North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Segal
authors.forename: C F
authors.initials: CF
authors.lastname: Barbas
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000737:chemistry*; D015723:Gene Library; D016678:Genome; D015894:Genome, Human; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D008024:Ligands; D011993:Recombinant Fusion Proteins