title: Synthetic peptide-based vaccine and diagnostic system for effective control of FMD.
abstract: We have designed synthetic peptides corresponding to two different regions of the genome of foot-and-mouth disease virus (FMDV) that are effective as (a) a vaccine or (b) a diagnostic reagent which differentiates convalescent from vaccinated animals, respectively. The peptide vaccine is based on a sequence from the prominent G-H loop of VP1, one of the four capsid proteins. The sequence was optimized by the inclusion of a cyclic constraint and adjoining sequences, and broader immunogenicity was obtained by the incorporation of consensus residues at hypervariable positions. The peptide also included a promiscuous T-helper epitope for effective immunogenicity in outbred populations of large animals.The diagnostic reagent, a peptide based on non-structural (NS) protein 3B, is used in immuno-assays for the detection of antibodies. Antibodies to this NS protein are present in the sera of infected animals but not in the sera of vaccinated animals. The VP1 peptide can be used in complementary immuno-assays for confirmation of NS test results and to monitor for vaccination. This system for differential diagnosis is important to establish the disease-free status of a country.
authors.affiliation: United Biomedical Inc., 25 Davids Drive, Hauppauge, NY 11788, U.S.A. cywang@unitedbiomedical.com
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.forename: T Y
authors.initials: TY
authors.lastname: Chang
authors.forename: A M
authors.initials: AM
authors.lastname: Walfield
authors.forename: J
authors.initials: J
authors.lastname: Ye
authors.forename: M
authors.initials: M
authors.lastname: Shen
authors.forename: M L
authors.initials: ML
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Lubroth
authors.forename: S P
authors.initials: SP
authors.lastname: Chen
authors.forename: M C
authors.initials: MC
authors.lastname: Li
authors.forename: Y L
authors.initials: YL
authors.lastname: Lin
authors.forename: M H
authors.initials: MH
authors.lastname: Jong
authors.forename: P C
authors.initials: PC
authors.lastname: Yang
authors.forename: N
authors.initials: N
authors.lastname: Chyr
authors.forename: E
authors.initials: E
authors.lastname: Kramer
authors.forename: F
authors.initials: F
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D010455:Peptides