Identification of rat EMAP, a delta-glutamate receptor binding protein.
While most subtypes of glutamate receptors have been studied extensively, less is known about the delta-glutamate receptors, delta1 and delta2. Although neither forms functional channels when expressed in heterologous cells, genetic analyses have demonstrated the physiological significance of delta2. We used the cytosolic C-terminus of the delta2 glutamate receptor subunit in a yeast two-hybrid screen of a rat brain cDNA library to identify delta-glutamate receptor binding proteins. We isolated rat EMAP, the rat homolog of a microtubule-associated protein initially isolated and characterized in echinoderms. Rat EMAP contains 10 WD-repeats, which are domains important for mediating protein-protein interactions in a wide variety of proteins. Rat EMAP binds to delta-glutamate receptor subunits within a 50-amino-acid segment of the delta C-terminus. It is widely expressed in both brain and peripheral tissues, including high expression in brainstem and enrichment in the postsynaptic density.
Ly
C Dune
CD
Laboratory of Neurochemistry, National Institutes of Health, Bethesda, Maryland 20892, USA.
Roche
Katherine W
KW
Lee
Ho-Ki
HK
Wenthold
Robert J
RJ
D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D017403:In Situ Hybridization; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding / Q000502:physiology; D021122:Protein Subunits; D051381:Rats; D017470:Receptors, Glutamate / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C115941:EMAP protein, Strongylocentrotus purpuratus; C485473:Eml2 protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D021122:Protein Subunits; D017470:Receptors, Glutamate