title: The glycoconjugate sugar residues of the sessile and motile cells in the thymus of normal and cyclosporin-A-treated rats: lectin histochemistry.
abstract: It is well known that cell surface glycoconjugates play a determinant role in cellular recognition, cell-to-cell adhesion and serve as receptor molecules. T-lymphocytes are in strict contact with the thymic epithelial cells, which control their process of maturation and proliferation. On the other hand the normal maturation of the epithelial cells is believed to be induced by T-lymphocytes. For these reasons we have studied the glycoconjugates saccharidic moieties of the sessile and motile cells in the thymus of normal male albino Wistar rats and their changes following cyclosporin-A treatment, using a battery of seven HRP-lectins. Cytochemical controls were performed for specificity of lectin-sugar reaction. Some sections were pre-treated with neuraminidase prior to staining with HRP-lectins. Our results have demonstrated, in the control rats, a large amount and a variety of terminal and subterminal oligosaccharides within and/or on the epithelial thymic cells and in macrophages. After cyclosporin-A treatment, among the thymic epithelial cells, the subcapsular, paraseptal and perivascular cells showed the loss of some sugar residues, which characterized the same cells in the intact thymus. Some hypotheses are reported on the role played by the glycoconjugate sugar residues in control and cyclosporin-A treated rats.
authors.affiliation: Department of Anatomy, Histology and Forensic Medicine, Policlinico di Careggi, Florence, Italy. gheri@unifi.it
authors.forename: G
authors.initials: G
authors.lastname: Gheri
authors.forename: S
authors.initials: S
authors.lastname: Gheri Bryk
authors.forename: R
authors.initials: R
authors.lastname: Riccardi
authors.forename: E
authors.initials: E
authors.lastname: Sgambati
authors.forename: M B
authors.initials: MB
authors.lastname: Cirri Borghi
mesh_terms: D000818:Animals; D001284:Atrophy; D050260:Carbohydrate Metabolism; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D016572:Cyclosporine / Q000494:pharmacology*; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D037102:Lectins; D008297:Male; D009439:Neuraminidase / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D016707:Tissue Fixation
chemical_list: D006001:Glycoconjugates; D007166:Immunosuppressive Agents; D037102:Lectins; D016572:Cyclosporine; D006735:Horseradish Peroxidase; D009439:Neuraminidase