Pharmacokinetic studies of methionine in rats using deuterium-labeled methionine quantitative assessment of remethylation.
The pharmacokinetics of methionine has been studied in rats by means of stable isotope methodology. After the i.v. bolus injection of [2H7]methionine (5 mg/kg body wt.), the plasma concentrations of [2H7]methionine, demethylated [2H4]homocysteine and remethylated [2H4]methionine were determined simultaneously with endogenous methionine and homocysteine by gas chromatography-mass spectrometry. The half-life for [2H7]methionine were 35.0 +/- 6.9 min. The appearance of the metabolites, [2H4]homocysteine and [2H4]methionine, in the plasma was very rapid. The fraction of [2H7]methionine that remethylated to [2H4]methionine through [2H4]homocysteine were 0.185 +/- 0.028. The administered [2H7]methionine did not influence the plasma levels of endogenous methionine and homocysteine. The present stable isotope methodology has made it possible to evaluate the pharmacokinetics of methionine, including the estimation of remethylation.
Shinohara
Y
Y
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan. sinohara@ps.toyaku.ac.jp
Hasegawa
H
H
Tagoku
K
K
Hashimoto
T
T
D000818:Animals; D019540:Area Under Curve; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D007275:Injections, Intravenous; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008745:Methylation; D051381:Rats; D017207:Rats, Sprague-Dawley
D008715:Methionine; D003903:Deuterium