Nisin and pediocin production on mussel-processing waste supplemented with glucose and five nitrogen sources.
OBJECTIVE
Optimization of bacteriocin production by L. lactis subsp. lactis CECT 539 and Ped. acidilactici NRRL B-5627 on mussel-processing wastes.


RESULTS
The concentrations of glucose and five nitrogen sources that optimize nisin and pediocin production were determined using a second order orthogonal factorial design. NH4Cl, glycine and glutamic acid were poor nitrogen sources. Enhanced pediocin and nisin productions were achieved in a medium supplemented with yeast extract or Bacto casitone.


CONCLUSIONS
Mussel-processing wastes could be successfully used as a culture medium for bacteriocin production at low production cost.


CONCLUSIONS
Average optimization led to a threefold increase in pediocin production (from 322 to 934 BU ml(-1)) and in nisin production (from 32 to 100 BU ml(-1)) when compared with the unsupplemented medium. For this reason, mussel-processing waste warrants further investigation due to its potential use as a cheap culture medium for upscaling bacteriocin production.
Guerra
N P
NP
Department of Biochemistry, Genetics and Immunology, Vigo University, Spain.
Pastrana
L
L
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods; D049872:Bivalvia; D003470:Culture Media; D005511:Food Handling; D005947:Glucose / Q000378:metabolism; D007220:Industrial Waste; D013294:Lactococcus lactis / Q000254:growth & development* / Q000378:metabolism; D009561:Nisin / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D010376:Pediococcus / Q000254:growth & development* / Q000378:metabolism
D001430:Bacteriocins; D003470:Culture Media; D007220:Industrial Waste; D009561:Nisin; D005947:Glucose; D009584:Nitrogen