title: Assessment of proliferative activity of canine dermal mast cells by bromodeoxyuridine and proliferating cell nuclear antigen labelling.
abstract: Cutaneous mast cells from skin biopsies of three healthy dogs and three dogs with atopic dermatitis were assessed for their proliferative potential using bromodeoxyuridine and proliferating cell nuclear antigen labelling. Mast cells isolated from the skin of two healthy dogs were also studied using bromodeoxyuridine labelling. Mast cells in skin biopsy specimens and mast cells isolated from the skin of healthy dogs did not incorporate bromodeoxyuridine. Two mast cells expressing proliferating cell nuclear antigen were seen around two superficial vessels in the dermis of one atopic dog. Epidermal cells, glandular epithelial cells, fibroblasts and endothelial cells incorporated bromodeoxyuridine and showed positive staining for proliferating cell nuclear antigen. These results suggest that canine mature mast cells do not proliferate in the dermis.
authors.affiliation: Departament de Medicina i Cirurgia Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. laura.ordeix@uab.es
authors.forename: L
authors.initials: L
authors.lastname: Ordeix
authors.forename: D
authors.initials: D
authors.lastname: Fondevila
authors.forename: F
authors.initials: F
authors.lastname: de Mora
authors.forename: A
authors.initials: A
authors.lastname: Fondati
authors.forename: L
authors.initials: L
authors.lastname: Ferrer
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002454:Cell Differentiation; D003876:Dermatitis, Atopic / Q000503:physiopathology / Q000662:veterinary*; D004283:Dog Diseases / Q000503:physiopathology*; D004285:Dogs / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D009687:Nuclear Proteins / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen; D012879:Skin Physiological Phenomena
chemical_list: D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine