title: Approaches to interval mapping of QTL in a multigeneration pedigree: the example of porcine chromosome 4.
abstract: Quantitative trait loci (QTLs) have been mapped in many studies of F2 populations derived from crosses between diverse lines. One approach to confirming these effects and improving the mapping resolution is genetic chromosome dissection through a backcrossing programme. Analysis by interval mapping of the data generated is likely to provide additional power and resolution compared with treating data marker by marker. However, interval mapping approaches for such a programme are not well developed, especially where the founder lines were outbred. We explore alternative approaches to analysis using, as an example, data from chromosome 4 in an intercross between wild boar and Large White pigs where QTLs have been previously identified. A least squares interval mapping procedure was used to study growth rate and carcass traits in a subsequent second backcross generation (BC2). This procedure requires the probability of inheriting a wild boar allele for each BC2 animal for locations throughout the chromosome. Two methods for obtaining these probabilities were compared: stochastic or deterministic. The two methods gave similar probabilities for inheriting wild boar alleles and, hence, gave very similar results from the QTL analysis. The deterministic approach has the advantage of being much faster to run but requires specialized software. A QTL for fatness and for growth were confirmed and, in addition, a QTL for piglet growth from weaning at 5 weeks up to 7 weeks of age and another for carcass length were detected.
authors.affiliation: Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, UK. s.knott@ed.ac.uk
authors.forename: S A
authors.initials: SA
authors.lastname: Knott
authors.forename: P E
authors.initials: PE
authors.lastname: Nyström
authors.forename: L
authors.initials: L
authors.lastname: Andersson-Eklund
authors.forename: S
authors.initials: S
authors.lastname: Stern
authors.forename: L
authors.initials: L
authors.lastname: Marklund
authors.forename: L
authors.initials: L
authors.lastname: Andersson
authors.forename: C S
authors.initials: CS
authors.lastname: Haley
mesh_terms: D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D002875:Chromosomes; D005260:Female; D005838:Genotype; D006579:Heterozygote; D007178:Inbreeding; D016018:Least-Squares Analysis; D008297:Male; D010375:Pedigree; D019655:Quantitative Trait, Heritable; D012044:Regression Analysis; D013269:Stochastic Processes; D013552:Swine / Q000235:genetics*