Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule.
OBJECTIVE
To study the expression of HLA-G on peripheral blood mononuclear cells (PBMC) from HIV-1-infected individuals in order to determine whether this molecule is induced as a consequence of HIV-1 infection.


METHODS
A total of 23 HIV-positive individuals in different stages of the disease were studied.


METHODS
Flow cytometric analysis and Western blot were used to measure HLA-G expression on PBMC obtained from HIV-positive and control individuals.


RESULTS
Most of the monocytes obtained from HIV-1-infected individuals express HLA-G, whereas only a very low proportion of monocytes from healthy individuals express this molecule. When T lymphocytes from HIV-1 infections were studied, it was found that 30% of them express HLA-G, whereas only 1% were HLA-G-positive in healthy individuals. HLA-G expression was also confirmed by Western blot using specific anti-HLA-G monoclonal antibodies.


CONCLUSIONS
The synthesis of HLA-G is increased in monocytes and certain T lymphocytes from HIV-1-infected individuals.
Lozano
José M
JM
Service of Immunology, Reina Sofía University Hospital, University of Cordoba, School of Medicine, C. Menendez Pidal s/n, 14004 Cordoba, Spain. fi1pemaj@uco.es
González
Rafael
R
Kindelán
José M
JM
Rouas-Freiss
Nathalie
N
Caballos
Rafael
R
Dausset
Jean
J
Carosella
Edgardo D
ED
Peña
José
J
D015153:Blotting, Western; D016022:Case-Control Studies; D002460:Cell Line; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006680:HLA Antigens / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I