title: Proliferation of intestinal crypt cells by gastrin-induced ornithine decarboxylase.
abstract: OBJECTIVE
  To determine whether the gastrin stimulated intestinal crypt cell (IEC-6) proliferation by induction of ornithine decarboxylase (ODC).
  
  
  METHODS
  IEC-6 cells were grown in DMEM containing 50 mL x L(-1) dialyzed fetal bovine serum for 24h and then were treated with gastrin. The proliferative capability of the cells was monitored subsequently on d 1, 2, 3, and 4 after treatment with MTT assay at aborbance 570 nm.The cellular ODC mRNA expression, ODC activity, and putrescine content were examined by RT-PCR method, radiometric technique and high-performance liquid chromatography(HPLC) analysis respectively after 12h of treatment.
  
  
  RESULTS
  On d1 after exposure of IEC-6 cells to pentagastrin, the proliferation increased initially and reached a peak on d3 at 250 microg x L(-1) concentration. Pentagastrin 500microg x L(-1) increased cell proliferation on day 1 and day 2, and then decreased. Compared with control group, pentagastrin 250 microg x L(-1) increased ODC mRNA level by 1.09-fold (P<0.05), ODC activity by 1.71-fold(P<0.01), and putrescine content 5.30-fold (P<0.01), respectively. Similarly, pentagastrin of 500 microg x L(-1) also increased ODC mRNA level by 1.16-fold (P<0.05), ODC activity 1.63-fold(P<0.05), and putrescine content 4.41-fold (P<0.01), respectively. But there was not significant difference between them.
  
  
  CONCLUSIONS
  Gastrin is an agent which promotes IEC-6 cell proliferation involved in regulating ODC activity mechanism.
authors.affiliation: Piei Institute,Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
authors.forename: Zi-Li
authors.initials: ZL
authors.lastname: Zhang
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D005755:Gastrins / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007413:Intestinal Mucosa / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D010418:Pentagastrin / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
chemical_list: D005755:Gastrins; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase; D010418:Pentagastrin; D011700:Putrescine