Antigenic cross-reactivity of nerve growth factors from diverse source: activity versus toxicity of NGF.
NGF used in these studies was from various sources: cobra venom (V-NGF), mouse submaxillary glands (M-NGF), honeybee venom (B-NGF), human serum (H-NGF), and cobra serum (CS-NGF). This investigation reports the antigenic cross-reactivity of NGFs from the above mentioned diverse sources. Antigenic cross-reactivity of NGFs was determined by immunological test, using anti-NGFs against V-NGF, M-NGF, B-NGF, and H-NGF. Results revealed that NGF was a conserved protein showing antigenic cross-reactivity among the NGFs from diverse source, except for NGFs from cobra and bee venoms. Anti B-NGF reacted poorly with cobra venom V-NGF and vice versa. Anti M-NGF showed higher antigenic reactivity with human serum H-NGF than with honeybee B-NGF and cobra serum CS-NGF. This research also reports that the toxicity of NGF is directly related to the biological activity of producing neurite outgrowth on PC12 cells. Cobra venom NGF produced neurites at 1-5 ng, and it was toxic at 10-20 ng on PC12 cells. After reducing the activity of NGF, it was found to be non-toxic to PC12 cells at 50 X concentration.
Lipps
Binie V
BV
Ophidia Products, Inc, Houston, Texas 77035, USA. bvl@ophidia.com
D000818:Animals; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D009414:Nerve Growth Factors / Q000276:immunology* / Q000633:toxicity*; D016501:Neurites / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D010673:Pheochromocytoma / Q000276:immunology / Q000473:pathology; D051381:Rats; D013045:Species Specificity
D009414:Nerve Growth Factors