title: Ion/ion chemistry as a top-down approach for protein analysis.
abstract: Developing methodology for analyzing complex protein mixtures in a rapid fashion is one of the most challenging problems facing analytical biochemists today. Recent advances in mass spectrometry for the analysis of intact proteins (i.e. the top-down approach) show great promise for rapid protein identification. The ion/ion chemistry approach for the detection and identification of target proteins in complex matrices, determination of fragmentation channels as a function of precursor ion charge state, and post-translational modification characterization are discussed with particular emphasis on tandem mass spectrometry of intact proteins.
authors.affiliation: Research Triangle Institute, 3040 Cornwallis Road, PO Box 12194, Research Triangle Park, NC 27709-2194, USA. stephensonjl@rti.org
authors.forename: James L
authors.initials: JL
authors.lastname: Stephenson
authors.forename: Scott A
authors.initials: SA
authors.lastname: McLuckey
authors.forename: Gavin E
authors.initials: GE
authors.lastname: Reid
authors.forename: J Mitchell
authors.initials: JM
authors.lastname: Wells
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Bundy
mesh_terms: D007477:Ions / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D007477:Ions; D011506:Proteins