Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.
OBJECTIVE
The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.


RESULTS
Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cultures of 492 intestinal homogenates and 27 drag swabs from 47 poultry flocks. The number of positive individual samples by real-time PCR and culture method was 65 (12.5%) and 35 (6.8%), respectively. The number of Salmonella-positive flocks was 13 (27.7%) by both methods. PCR detection required 25 min for up to 32 samples. Melting curve analysis revealed the Tm for Salmonella-specific PCR product as 87 +/- 1 degrees C.


CONCLUSIONS
Implementation of real-time PCR to tetrathionate broth enrichment step reduces the Salmonella detection time to 18 h and 25 min. Isolation of Salmonella should be carried out with PCR to determine the serovar.


CONCLUSIONS
Real-time PCR is a powerful tool in rapid and accurate Salmonella monitoring in poultry companies, together with standard bacteriology.
Eyigor
A
A
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Uludag University, Gorukle Kampusu, 16043 Bursa, Turkey.
Carli
K T
KT
Unal
C B
CB
D000818:Animals; D003470:Culture Media; D016133:Polymerase Chain Reaction / Q000379:methods*; D011200:Poultry / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D003470:Culture Media