title: Molecular analysis of Neorickettsia risticii in adult aquatic insects in Pennsylvania, in horses infected by ingestion of insects, and isolated in cell culture.
abstract: Upon ingestion of adult aquatic insects, horses developed clinical signs of Potomac horse fever, and Neorickettsia risticii was isolated from the blood. 16S rRNA and 51-kDa antigen gene sequences from blood, isolates, and caddis flies fed to the horses were identical, proving oral transmission of N. risticii from caddis flies to horses.
authors.affiliation: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1092, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Mott
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Seaton
authors.forename: Carol
authors.initials: C
authors.lastname: Martin
authors.forename: Stephen
authors.initials: S
authors.lastname: Reed
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Rikihisa
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D005247:Feeding Behavior; D006734:Horse Diseases / Q000382:microbiology* / Q000635:transmission; D006736:Horses / Q000502:physiology*; D007313:Insecta / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D010414:Pennsylvania; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012287:Rickettsiaceae / Q000145:classification* / Q000235:genetics*; D012288:Rickettsiaceae Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*
chemical_list: C415977:51-kDa antigen, Ehrlichia risticii; D000947:Antigens, Helminth; D003470:Culture Media; D012336:RNA, Ribosomal, 16S