title: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule.
abstract: OBJECTIVE
  To study the expression of HLA-G on peripheral blood mononuclear cells (PBMC) from HIV-1-infected individuals in order to determine whether this molecule is induced as a consequence of HIV-1 infection.
  
  
  METHODS
  A total of 23 HIV-positive individuals in different stages of the disease were studied.
  
  
  METHODS
  Flow cytometric analysis and Western blot were used to measure HLA-G expression on PBMC obtained from HIV-positive and control individuals.
  
  
  RESULTS
  Most of the monocytes obtained from HIV-1-infected individuals express HLA-G, whereas only a very low proportion of monocytes from healthy individuals express this molecule. When T lymphocytes from HIV-1 infections were studied, it was found that 30% of them express HLA-G, whereas only 1% were HLA-G-positive in healthy individuals. HLA-G expression was also confirmed by Western blot using specific anti-HLA-G monoclonal antibodies.
  
  
  CONCLUSIONS
  The synthesis of HLA-G is increased in monocytes and certain T lymphocytes from HIV-1-infected individuals.
authors.affiliation: Service of Immunology, Reina Sofía University Hospital, University of Cordoba, School of Medicine, C. Menendez Pidal s/n, 14004 Cordoba, Spain. fi1pemaj@uco.es
authors.forename: José M
authors.initials: JM
authors.lastname: Lozano
authors.forename: Rafael
authors.initials: R
authors.lastname: González
authors.forename: José M
authors.initials: JM
authors.lastname: Kindelán
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rouas-Freiss
authors.forename: Rafael
authors.initials: R
authors.lastname: Caballos
authors.forename: Jean
authors.initials: J
authors.lastname: Dausset
authors.forename: Edgardo D
authors.initials: ED
authors.lastname: Carosella
authors.forename: José
authors.initials: J
authors.lastname: Peña
mesh_terms: D015153:Blotting, Western; D016022:Case-Control Studies; D002460:Cell Line; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006680:HLA Antigens / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I