A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts.
A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCl to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38 +/- 4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.
Guerrieri
A
A
Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy.
Monaci
L
L
Quinto
M
M
Palmisano
F
F
D015374:Biosensing Techniques; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004209:Disposable Equipment; D004563:Electrochemistry; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds; D012989:Soil Pollutants / Q000032:analysis*
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D012989:Soil Pollutants