Overexpression of the secreted factor Mig30 expressed in the Spemann organizer impairs morphogenetic movements during Xenopus gastrulation.
The Spemann organizer secretes several antagonists of growth factors during gastrulation. We describe a novel secreted protein, Mig30, which is expressed in the anterior endomesoderm of the Spemann organizer. Mixer-inducible gene 30 (Mig30) was isolated as a target of Mixer, a homeobox gene required for endoderm development. The Mig30 gene encodes a secreted protein containing a cysteine-rich domain and an immunoglobulin-like domain that belongs to the insulin-like growth factor-binding protein family. Overexpression of Mig30 in the dorsal region results in the retardation of morphogenetic movements during gastrulation and leads to microcephalic embryos. Overexpression of Mig30 also inhibits activin-induced elongation of ectodermal explants without affecting gene expression patterns in mesoderm and endoderm. These results suggest that Mig30 is involved in the regulation of morphogenetic movements during gastrulation in the extracellular space of the Spemann organizer.
Hayata
Tadayoshi
T
Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Tanegashima
Kosuke
K
Takahashi
Shuji
S
Sogame
Asako
A
Asashima
Makoto
M
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003545:Cysteine / Q000737:chemistry; D018076:DNA, Complementary / Q000378:metabolism; D004475:Ectoderm / Q000378:metabolism; D005697:Galactosides / Q000378:metabolism; D005775:Gastrula / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007211:Indoles / Q000378:metabolism; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D020897:Organizers, Embryonic / Q000196:embryology*; D010957:Plasmids / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014981:Xenopus; D029867:Xenopus Proteins / Q000096:biosynthesis* / Q000235:genetics*
D018076:DNA, Complementary; D005697:Galactosides; D018398:Homeodomain Proteins; D007211:Indoles; D018969:Insulin-Like Growth Factor Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C461679:Mig30 protein, Xenopus; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D029867:Xenopus Proteins; C100866:cer1 protein, Xenopus; C405705:mixer protein, Xenopus; D003545:Cysteine; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside