Opposite actions of brain-derived neurotrophic factor and neurotrophin-3 on firing features and ion channel composition of murine spiral ganglion neurons.
It is now well established that sensory neurons and receptors display characteristic morphological and electrophysiological properties tailored to their functions. This is especially evident in the auditory system, where cells are arranged tonotopically and are highly specialized for precise coding of frequency- and timing-dependent auditory information. Less well understood, however, are the mechanisms that give rise to these biophysical properties. We have provided insight into this issue by using whole-cell current-clamp recordings and immunocytochemistry to show that BDNF and NT-3, neurotrophins found normally in the cochlea, have profound effects on the firing properties and ion channel distribution of spiral ganglion neurons in the murine cochlea. Exposure of neurons to BDNF caused all neurons, regardless of their original cochlear position, to display characteristics of the basal neurons. Conversely, NT-3 caused cells to show the properties of apical neurons. These results are consistent with oppositely oriented gradients of these two neurotrophins and/or their high-affinity receptors along the tonotopic map, and they suggest that a combination of neurotrophins are necessary to establish the characteristic firing features of postnatal spiral ganglion neurons.
Adamson
Crista L
CL
W. M. Keck Center for Collaborative Neuroscience and Department of Cell Biology and Neuroscience, Nelson Laboratories, Rutgers University, Piscataway, New Jersey 08854-8082, USA.
Reid
Michael A
MA
Davis
Robin L
RL
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002478:Cells, Cultured; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D051662:Kv1.1 Potassium Channel; D051036:Large-Conductance Calcium-Activated Potassium Channels; D051379:Mice; D008808:Mice, Inbred CBA; D009474:Neurons / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D009479:Neuropeptides / Q000187:drug effects / Q000378:metabolism; D020933:Neurotrophin 3 / Q000494:pharmacology*; D009928:Organ Specificity; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism; D024681:Potassium Channels, Calcium-Activated; D024642:Potassium Channels, Voltage-Gated; D011930:Reaction Time / Q000187:drug effects; D051672:Shal Potassium Channels; D051670:Shaw Potassium Channels; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D007473:Ion Channels; C496947:Kcna1 protein, mouse; D051036:Large-Conductance Calcium-Activated Potassium Channels; D009479:Neuropeptides; D020933:Neurotrophin 3; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels; D051670:Shaw Potassium Channels; D051662:Kv1.1 Potassium Channel