title: Occurrence and transferability of beta-lactam resistance in Enterobacteriaceae isolated in Children's University Hospital in Bratislava.
abstract: Occurrence and transferability of beta-lactam resistance in 30 multi-resistant Escherichia coli, Klebsiella spp., Enterobacter spp., Pantoea agglomerans, Citrobacter freundii and Serratia marcescens strains isolated from children between 0 and 3 years of age is presented. The strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30), ceftriaxone (30), ceftazidime (30) and aztreonam (28), but susceptible to cefepime (30) and imipenem (26). Twenty-eight of 30 isolates possessed a transferable resistance confirmed by conjugation and isolation of 79-89-kb plasmids. The beta-lactam resistance was due to production of beta-lactamases and ceftazidime proved to be stronger beta-lactamase inductor than ceftriaxone. Twenty-five clinical isolates expressed transferable extended spectrum beta-lactamases, and chromosomally encoded AmpC beta-lactamase.
authors.affiliation: Department of Microbiology and Virology, Faculty of Science, Comenius University, 842 15 Bratislava, Slovakia. bujdakova@fns.uniba.sk
authors.forename: H
authors.initials: H
authors.lastname: Bujdáková
authors.forename: J
authors.initials: J
authors.lastname: Hanzen
authors.forename: S
authors.initials: S
authors.lastname: Jankovicová
authors.forename: J
authors.initials: J
authors.lastname: Klimácková
authors.forename: M
authors.initials: M
authors.lastname: Moravcíková
authors.forename: P
authors.initials: P
authors.lastname: Milosovic
authors.forename: D
authors.initials: D
authors.lastname: Michálková-Papajová
authors.forename: J
authors.initials: J
authors.lastname: Kallová
authors.forename: A
authors.initials: A
authors.lastname: Jakab
authors.forename: M
authors.initials: M
authors.lastname: Kettner
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology; D002675:Child, Preschool; D003227:Conjugation, Genetic; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D006785:Hospitals, University; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D018154:Slovakia / Q000453:epidemiology; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D002442:Ceftazidime; D001618:beta-Lactamases