title: Anti-inflammatory effects of IL-4 and IL-10 on human polymorphonuclear leukocytes.
abstract: Inflammatory responses are strictly regulated by coordination of pro-inflammatory and anti-inflammatory mediators. Interleukin-4 (IL-4) and interleukin-10 (IL-10) have typically the biologic anti-inflammatory effects on monocytes, but uncertain effects on polymorphonuclear leukocytes (PMNs). The PMNs are the first line of cellular response for host defense during acute inflammation. To modify hyper-inflammatory reaction with biologic anti-inflammatory mediators, we have determined the biologic anti-inflammatory activities of IL-4 and IL-10 on human PMNs. Human PMNs were pretreated with IL-4 or IL-10 and then stimulated with formyl methionyl leucyl phenylalanine (fMLP) for times indicated. The level of H2O2, interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) were determined in the each cell free supernatants. fMLP plays the role of a typical pro-inflammatory agent and, at least in determined conditions, down-regulated TNF release. IL-4 acts as an anti-inflammatory mediator but IL-10 did not show its anti-inflammatory activities on fMLP-stimulated human PMNs. IL-4 and IL-10 have different anti-inflammatory mechanisms. Perhaps, IL-10 needs co-factors to act as an anti-inflammatory mediator.
authors.affiliation: Department of Emergency Medicine, College of Medicine, Korea University, Seoul, Korea. kuedlee@korea.ac.kr
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Yun Sik
authors.initials: YS
authors.lastname: Hong
authors.forename: Chung Min
authors.initials: CM
authors.lastname: Chun
authors.forename: Jun Dong
authors.initials: JD
authors.lastname: Moon
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: In Chul
authors.initials: IC
authors.lastname: Jung
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Yoon
authors.forename: Be An
authors.initials: BA
authors.lastname: Lee
authors.forename: Sung Woo
authors.initials: SW
authors.lastname: Moon
authors.forename: Sung Hyuk
authors.initials: SH
authors.lastname: Choi
authors.forename: Chul Kyu
authors.initials: CK
authors.lastname: Moon
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016753:Interleukin-10 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000378:metabolism; D007424:Intracellular Fluid; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide