title: Advances in proteome analysis by mass spectrometry.
abstract: Proteome characterization using mass spectrometry is essential for the systematic investigation of biological systems and for the study of gene function. Recent advances in this multifaceted field have occurred in four general areas: protein and peptide separation methodologies; selective labeling chemistries for quantitative measurement of peptide and protein abundances; characterization of post-translational protein modifications; and instrumentation.
authors.affiliation: Institute for Systems Biology, 4225 Roosevelt Way NE, Suite 200, Seattle, WA 98105, USA. tgriffin@systemsbiology.org
authors.forename: T J
authors.initials: TJ
authors.lastname: Griffin
authors.forename: D R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: R
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
chemical_list: D010455:Peptides; D011506:Proteins