title: Levels of GM-CSF, IL-3, and IL-6 in fluid and tissue from human radicular cysts.
abstract: Cytokines released by immune system cells play an important role in cyst enlargement. This study aimed to determine, by ELISA, the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), and IL-6 in fluid and tissue from human radicular cysts. GM-CSF was found in 42.8% of the fluid samples (164.3 pg/mL) and IL-6 in 92.8% (641.4 pg/mL). No IL-3 was detected in any fluid samples. In the tissue samples, 28.6% were positive for IL-3 (369.2 pg/mL), 86.4% for IL-6 (92.4 pg/mL), and 95.8% for GM-CSF (200.5 pg/mL). It can be concluded that GM-CSF and IL-6 were widely found in the fluid and tissue samples. In contrast, IL-3 was found only in the cystic tissue, even though in few lesions. These cytokines may contribute to the inflammation, cystic growth, and bone resorption that characterize cystic lesions.
authors.affiliation: Division of Immunology, Institute of Biomedical Sciences, Federal University of Uberlândia, MG, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Gervásio
authors.forename: D A O
authors.initials: DA
authors.lastname: Silva
authors.forename: E A
authors.initials: EA
authors.lastname: Taketomi
authors.forename: C J A
authors.initials: CJ
authors.lastname: Souza
authors.forename: S S J
authors.initials: SS
authors.lastname: Sung
authors.forename: A M
authors.initials: AM
authors.lastname: Loyola
mesh_terms: D016301:Alveolar Bone Loss / Q000276:immunology; D020420:Cyst Fluid / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis*; D006801:Humans; D007377:Interleukin-3 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D011842:Radicular Cyst / Q000737:chemistry / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D007377:Interleukin-3; D015850:Interleukin-6; D016178:Granulocyte-Macrophage Colony-Stimulating Factor