title: Effects of pH and temperature on benzo[a]pyrene-DNA, -protein, and -lipid adducts in primary rat hepatocytes.
abstract: The effects of pH and temperature on benzo[a]pyrene(BaP)-DNA, -protein, and -lipid adducts were compared in rat hepatocytes treated with [3H]-BaP. In primary rat hepatocytes, BaP-DNA, -protein, and -lipid adducts were formed in a concentration-dependent manner (0.31-2.80 mmol BaP), and the formation of BaP-lipid adducts, specifically triglyceride (TG) adducts, correlated well with that of DNA or protein adducts. As incubation time increased (1-24 h), the formation of DNA or protein adducts was also elevated, while BaP-TG adducts decreased after 3 h of reaction. pH had no effect on the formation of [3H]-BaP-DNA, and -protein adducts, and the TG adducts decreased with increasing pH (3.4-11.4). With an increase in incubation temperature (30-45 degrees C), on the other hand, the adduct formation was increased with increasing temperature up to 40 degrees C and then decreased sharply. These results suggest that the physicochemical properties of BaP-DNA, -protein, and -TG adducts might be important to consider when cells or humans who have been exposed to BaP are monitored for biomarkers.
authors.affiliation: Division of Toxicology, School of Pharmacy, Sungkyunkwan University, Kyunggi-Do, Suwon, Korea.
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Park
authors.forename: Yo An
authors.initials: YA
authors.lastname: Park
authors.forename: Byung Mu
authors.initials: BM
authors.lastname: Lee
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers; D002478:Cells, Cultured; D018736:DNA Adducts / Q000187:drug effects* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature
chemical_list: D015415:Biomarkers; D018736:DNA Adducts; D011506:Proteins; D001564:Benzo(a)pyrene