Lipid modification in mouse peritoneal macrophages after chronic cadmium exposure.
The effect of cadmium (Cd) exposure through drinking water on lipid status in mouse peritoneal macrophages (pM) was studied. After 2 months, adult male Balb/c mice that had drunk water with 15 ppm of Cd, showed tissue damage mediated by oxidative stress, as assessed by serum measuring of tissue damage and lipoperoxidation indicators. Resident pM obtained from Cd-exposed mice showed diminution in total lipids, total cholesterol, free cholesterol/esterified cholesterol ratio (FC/EC) and phospholipids in relation to control pM. On a percentage basis, the phospholipid composition showed that phosphatidylcholine (PC) and phosphatidylglycerol decreased, phosphatidylethanolamine (PE) increased, while phosphatidylinositol, sphingomyeline and phosphatidylserine did not change. The incorporation in vitro of [14C]-methyl-choline and [14C]-phosphorylcholine, as well as the activity of regulatory enzyme CTP-phosphocholine cytidylyltransferase, decreased in PC after 60 min. The incorporation of [14C]-linoleic acid increased after 1 h and the incorporation of [14C]-ethanolamine increased after 90 min in PC. The incorporation in vitro of [3H]-cholesterol in total lipids decreased after 120 min of incubation. Besides, the stearic acid and arachidonic acid content increased, while the contents of palmitoleic acid and linoleic acid decreased. Chronic Cd exposure alters the lipid composition in resident pM of Balb/c mice.
Ramirez
Dario C
DC
Laboratory of Molecular Biochemistry (CONICET), Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Avenida Ejercito de los Andes 950, 5700 San Luis, Argentina. dramirez@unsl.edu.ar
Gimenez
María S
MS
D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002469:Cell Separation; D002784:Cholesterol / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D019787:Linoleic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009713:Nucleotidyltransferases / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D012316:RNA Nucleotidyltransferases; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014881:Water Supply
D004983:Ethanolamines; D005227:Fatty Acids; D007202:Indicators and Reagents; D008055:Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D002104:Cadmium; D002784:Cholesterol; D019787:Linoleic Acid; D009713:Nucleotidyltransferases; D012316:RNA Nucleotidyltransferases; C020126:Ethanolamine-phosphate cytidylyltransferase