[Influence of exogenous RNA upon expression of mouse albumin gene and its sensitivity to DNase I].
Cultured mouse fibroblasts were treated with rabbit liver RNA. Rat liver RNA was injected into mouse prostates. Influence of exogenous RNA upon expression of mouse albumin gene was detected by immunohistochemical staining. Nucleuses of cultured mouse fibroblasts treated with different RNAs were isolated and digested with DNase I. Mouse albumin gene was amplified by PCR to detect levels of its digestion. It was found that exogenous RNAs could increase the sensitivity of mouse albumin gene to DNase I digestion and promote its expression.
Zhang
Hong-Yan
HY
Department of Laboratory Animal, Heibei Medical University, Shijiazhuang 050017, China.
Liu
Ai-Jing
AJ
Liu
Fu-Ying
FY
Wang
Xiu-Fang
XF
Lü
Zhan-Jun
ZJ
D000418:Albumins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D003850:Deoxyribonuclease I / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005399:Fishes; D015870:Gene Expression / Q000187:drug effects*; D005796:Genes / Q000187:drug effects; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D051379:Mice; D011467:Prostate / Q000166:cytology / Q000187:drug effects; D012313:RNA / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats
D000418:Albumins; D012313:RNA; D003850:Deoxyribonuclease I