Impact of the lymphoma idiotype on in vivo tumor protection in a vaccination model based on targeting antigens to antigen-presenting cells.
Trioma cell vaccination is a potent new immunologic approach for the therapy of malignant B-cell lymphoma. It is based on targeting tumor antigens to internalizing receptors on antigen-presenting cells (APCs). Tumor cells are fused to an APC-specific hybridoma, where they are converted to trioma cells that include potentially all lymphoma-derived antigens and that express the APC-binding arm. In this study, the mechanisms of trioma-mediated tumor immunity in immunocompetent mice were dissected, and it was shown in this model system that humoral anti-idiotypic immunity is indeed detectable after idiotype-specific immunization but that it does not reflect the degree of tumor protection obtained in vivo. Immunization against the idiotype alone was not sufficient for efficient tumor rejection in vivo. Targeting tumor antigens to APCs is only successful in terms of inducing tumor protection when designed as a polyvalent vaccination protocol.
Kronenberger
Konrad
K
GSF-Institut für Molekulare Immunologie, Munich, Germany.
Dieckmann
Andreas
A
Selmayr
Michael
M
Strehl
John
J
Wahl
Ulrich
U
Lindhofer
Horst
H
Kraal
Georg
G
Mocikat
Ralph
R
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000592:standards; D005260:Female; D006825:Hybridomas / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011961:Receptors, Fc / Q000276:immunology; D015996:Survival Rate; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology / Q000637:transplantation
D000888:Antibodies, Anti-Idiotypic; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D011961:Receptors, Fc