[Fluorescence in situ hybridization study on the chromosome 17 aneuploidy of human oocytes failing to fertilize in vitro].
OBJECTIVE
Compare two methods of preparing human unfertilized oocytes interphase nucleus and analyze the relationship between the different in vitro fertilization(IVF) indications, ovarian stimulation protocols, women's age and frequency of chromosome 17 aneuploidy.


METHODS
Tarkowski's air-drying method(3:1 methanol:acetic acid) and Coonen's 0.1% Tween 20/0.01 mol/L HCl method were used to fix human unfertilized oocytes interphase nucleus, and telomeric probe of 17qter was used by standard fluorescence in situ hybridization(FISH) procedures to confirm chromosome 17qter aneuploidy.


RESULTS
Of 36 human unfertilized oocytes, 24 were haploid (66.7%), 7 were disomic (19.44%), 5 were trisomic (13.89%). The overall frequency of aneuploidy was 33.3%. There were no differences between the protocols characterized by different maternal age, IVF indication, ovarian stimulation.


CONCLUSIONS
Tarkowski's air dry method is as good as the method of Coonen's, but the latter method can avoid the smell pollution of the methanol and acetic acid, and it is easy to operate. The chromosome 17 aneuploidy is one of the factors to cause in vitro fertilization failure of human oocytes.
Zhang
Qunfang
Q
Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South University, Changsha, Hunan, 410078 P. R. China. lgxdirector@sina.com
Lu
Guangxiu
G
D000328:Adult; D000782:Aneuploidy; D002467:Cell Nucleus / Q000235:genetics / Q000648:ultrastructure; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics* / Q000648:ultrastructure; D015342:DNA Probes; D004171:Diploidy; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006238:Haploidy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D007621:Karyotyping; D008856:Microscopy, Fluorescence / Q000379:methods; D010062:Ovulation Induction / Q000379:methods; D010063:Ovum / Q000502:physiology*; D014314:Trisomy
D015342:DNA Probes