title: The effects of excessive heat on heat-activated membrane currents in cultured dorsal root ganglia neurons from neonatal rat.
abstract: The effects of high temperature (53-61 degrees C) on membrane currents (I(heat)) or depolarization (V(heat)) induced by noxious heat were studied in cultured dorsal root ganglia neurons from neonatal rats using the whole cell patch clamp technique. I(heat) or V(heat) produced by 3 s ramps of increasing temperature between 43 and 50 degrees C exhibited a fast slope (Q10>10) that was similar both during rising and falling temperature (n=85). Temperatures exceeding 52 degrees C resulted in slowdown in the recovery of I(heat), and the threshold for inducing I(heat) was shifted to lower temperatures in successive trials. These high temperatures (54-60 degrees C) caused a linear and incomplete recovery of I(heat) (Q10 decreased to <5; 4.5 +/- 0.4; n=17) and in successive trials the threshold of I(heat) decreased to temperatures close to that in the bath. The neurons, however, remained sensitive to capsaicin and to decreased extracellular pH. It is suggested that exposure of nociceptive neurons to excessive noxious heat results in an irreversible decrease of the energy barrier between the resting and activated state of the protein structures responsible for generation of I(heat). This may explain the sensitization of nociceptors after heat injury.
authors.affiliation: Institute of Physiology, Academy of Sciences of Czech Republic, 142 20 Prague 4, Vï#x0301;deňská 1083, Czech Republic Institut f. Physiologie u. Experimentelle Pathophysiologie, Universitaetsstrasse 17, D-91054 Erlangen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Lyfenko
authors.forename: V
authors.initials: V
authors.lastname: Vlachová
authors.forename: L
authors.initials: L
authors.lastname: Vyklický
authors.forename: I
authors.initials: I
authors.lastname: Dittert
authors.forename: M
authors.initials: M
authors.lastname: Kress
authors.forename: P W
authors.initials: PW
authors.lastname: Reeh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002478:Cells, Cultured; D002794:Choline / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology*; D006358:Hot Temperature; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000166:cytology / Q000502:physiology*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology*; D018697:Nootropic Agents / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018697:Nootropic Agents; D002794:Choline; D002211:Capsaicin; D002118:Calcium