[Changes in myelin sheath and axon of spinal cord in X-irradiation injured rats].
OBJECTIVE
To investigate the X-irradiation effect on the myelin sheath and axon of the spinal cord.


METHODS
60 Wistar adult rats receiving hemisection in the thoracic spinal cord were made into models of spinal injury. Among these rats, 30 in the test group received X-irradiation of 35-Gy the day after operation, while 30 in the control group only received hemisection. Operative spinal cord was subjected to immunohistochemistry and hyper-microscopic observation.


RESULTS
In the test group, injury to the myelin sheath of the upper and lower lesion and the number of neuro-fibrotic positive axon increased. In the control group the number of myelin sheath below the injured section decreased. Under electronic microscope, demyelination was found in the spinal cord and new-born tiny neuro-fibrotic tracts were seen below the lesion.


CONCLUSIONS
X-irradiation can make spinal cord demyelinate and promote the increase of neuro-fibrotic positive axon.
Bai
X
X
Department of Orthopaedic Surgery, First Clinical College China Medical University, Shengyang 110001, China.
Fan
G
G
Yuan
Y
Y
D000818:Animals; D001369:Axons / Q000473:pathology / Q000528:radiation effects*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D009186:Myelin Sheath / Q000473:pathology / Q000528:radiation effects*; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000473:pathology / Q000532:radiotherapy*