title: Conservation of the T-cell receptor alpha/delta linkage in the teleost fish Tetraodon nigroviridis.
abstract: T-cell specific receptors (TCR) are present in all groups] from the jawed vertebrates to the mammals. In teleosts, however, the genes encoding the gamma- and delta-chains have not yet been found, the alpha- and beta-chains have been characterized mainly at the expression level, and genomic organization of these loci remains largely unknown. Here we describe both the genomic organization of the TCR alpha/delta locus in Tetraodon nigroviridis and the transcription of TCRA and TCRD. The TCR alpha/delta locus consists of 13 V alpha/delta segments, a Calpha gene, and 12 Jalpha segments, followed by a Cdelta gene, two Jdelta segments, and several Ddelta segments. However, the genomic organization found in this teleost differs significantly from that which has been observed in mammals and birds: a common set of V segments is used to generate either an alpha- or a delta-chain by genomic inversion, and the size of the locus is small in this vertebrate.
authors.affiliation: Genoscope/Centre national de séquençage et CNRS-UMR 8030, 2 rue Gaston Crémieux, Evry Cedex 06, F-91057, France. fischer@genoscope.cns.fr
authors.forename: Cécile
authors.initials: C
authors.lastname: Fischer
authors.forename: Laurence
authors.initials: L
authors.lastname: Bouneau
authors.forename: Catherine
authors.initials: C
authors.lastname: Ozouf-Costaz
authors.forename: Tatjana
authors.initials: T
authors.lastname: Crnogorac-Jurcevic
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
authors.forename: Alain
authors.initials: A
authors.lastname: Bernot
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D017124:Conserved Sequence; D004247:DNA; D019143:Evolution, Molecular; D005432:Flounder / Q000235:genetics; D015723:Gene Library; D019673:Genes, T-Cell Receptor alpha / Q000235:genetics*; D019676:Genes, T-Cell Receptor delta / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D023721:Tetraodontiformes / Q000235:genetics*
chemical_list: D004247:DNA