title: Cell surface phenotype and cytokine secretion in Caco-2 cell cultures: increased RANTES production and IL-2 transcription upon stimulation with IL-1beta.
abstract: Caco-2 is a colonic tumour cell line which, when cultured, spontaneously exhibits enterocyte-like characteristics. Given the difficulties in maintaining long-lasting cultures of enterocytes, this cell line may be a suitable in vitro model to carry out experiments trying to delineate the involvement of enterocytes in local immune responses, and their role in pathology. It seems then reasonable to obtain a detailed immune analysis of Caco-2, and compare it with available data on enterocytes. Cytofluorometry revealed several leukocyte markers on Caco-2, present also on human enterocytes. These markers include surface proteases (CD10, CD13 and CD26), antigen-presenting cell markers (CD13, CD14, CD35 and CD63), integrins (CD18 and CD61), epithelial/endothelial markers (CD21, CD31, CD47 and CD59) and finally, CD25 and CD28. In contrast to enterocytes, HLA-class 11 molecules are not found on Caco-2, whether resting or gamma-IFN-stimulated. Moreover, culture experiments with allogeneic lymphocytes revealed that Caco-2 cells were unable to induce their proliferation. Cytokine analysis showed an increased RANTES synthesis and IL-2 transcription upon stimulation with IL-1beta. Finally, amongst RANTES receptors, CCR1 is found on Caco-2 cells, whereas CCR3 and CCR5 are not.
authors.affiliation: Inmunologia, Fac Medicina, Universidad Complutense de Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Rodriguez-Juan
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Blas
authors.forename: A P
authors.initials: AP
authors.lastname: Valeri
authors.forename: N
authors.initials: N
authors.lastname: Aguilera
authors.forename: A
authors.initials: A
authors.lastname: Arnaiz-Villena
authors.forename: A
authors.initials: A
authors.lastname: Pacheco-Castro
authors.forename: J M
authors.initials: JM
authors.lastname: Martin-Villa
mesh_terms: D000938:Antigen-Presenting Cells / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D018938:Caco-2 Cells / Q000276:immunology* / Q000378:metabolism; D018946:Chemokine CCL5 / Q000096:biosynthesis*; D010450:Endopeptidases / Q000032:analysis; D004727:Endothelium / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit; D010641:Phenotype; D018123:Receptors, Interleukin / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D018946:Chemokine CCL5; D006684:HLA-DR Antigens; C508017:IL2RA protein, human; D016023:Integrins; D007375:Interleukin-1; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D018123:Receptors, Interleukin; D007371:Interferon-gamma; D010450:Endopeptidases