Identification of lactic acid bacteria from spoiled, vacuum-packaged 'gravad' rainbow trout using ribotyping.
A total of 296 lactic acid bacteria (LAB) isolated from spoiled, vacuum-packaged 'gravad' rainbow trout stored at 3 and 8 degrees C were characterised and identified using a molecular approach. The isolates were initially grouped according to their HindIII restriction endonuclease profiles and further identified to species level using an rRNA gene restriction pattern (ribotype) identification database. Lactobacillus sakei, L. curvatus and Carnobacterium piscicola were the three main species detected. Only one isolate was identified as C. divergens. Most of the carnobacteria were found in the samples stored at 3 degrees C. The relative proportion of L. sakei was higher in the samples stored at 8 degrees C.
Lyhs
Ulrike
U
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Finland. ulrike.lyhs@helsinki.fi
Korkeala
Hannu
H
Björkroth
Johanna
J
D000818:Animals; D015247:Deoxyribonuclease HindIII / Q000737:chemistry; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D018857:Food Packaging; D005519:Food Preservation; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000737:chemistry; D015183:Restriction Mapping; D021521:Ribotyping; D013696:Temperature; D014618:Vacuum
D012335:RNA, Ribosomal; D015247:Deoxyribonuclease HindIII