[Effect of various methods of tissue storage on split-skin viability at 4 degrees C].
OBJECTIVE
To study various tissues and organs storage solutions (liver, kidney and corneal) at 4 degrees C for the storage of skin and the viability of skins stored in these solutions at correspondent condition.


METHODS
Five experiment groups investigated for storing porcine split-skin at 4 degrees C were MEM, UW, MEM plus CS, UW-R and MEM-R. A new assay, WST-1, was applied to evaluate skin viability at 1, 3, 5, 7, 10 and 14 day during storage respectively.


RESULTS
Skin viability can all be protected by these three solutions. The effect of UW solution was superior to MEM. UW-R and MEM-R were superior to MEM and UW.


CONCLUSIONS
The method of UW-R which replaces medium routinely may be an optimal method of storing skin at 4 degrees C.
Jia
X
X
Burn institute, 304th Hospital of People's Liberation Army, Beijing 100037.
Yang
H
H
Liss
K
K
McGann
L E
LE
Tredget
E E
EE
D000818:Animals; D002470:Cell Survival; D012034:Refrigeration / Q000379:methods* / Q000592:standards; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D012879:Skin Physiological Phenomena; D013552:Swine; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods* / Q000592:standards