title: Glutathione-dependent metabolism of the antitumor agent sulofenur. Evidence for the formation of p-chlorophenyl isocyanate as a reactive intermediate.
abstract: The antitumor agent sulofenur (LY186641), which has shown promising activity against a wide range of cancers, causes hemolytic anemia and methemoglobinemia at dose-limiting toxicities. The antitumor and toxicological mechanism(s) of action of the drug is (are) not well understood, but unlike other antineoplastic agents, sulofenur does not interfere with DNA, RNA, or protein synthesis, or with polynucleotide function. In the present study, we evaluated the hypothesis that sulofenur undergoes bioactivation in vivo to generate p-chlorophenyl isocyanate (CPIC), which could carbamoylate biological macromolecules directly or form a conjugate with glutathione (GSH) which would serve as a latent form of CPIC. The objectives of this study, therefore, were to determine if the GSH and N-acetylcysteine conjugates of CPIC were excreted into bile and urine, respectively, after an i.p. dose of sulofenur to rats. In addition, the chemical stability and thiol exchange properties of these S-linked conjugates were determined. The results of this study indicate that sulofenur does undergo metabolism in vivo to yield the GSH conjugate of CPIC, and that this conjugation reaction is reversible and subject to thiol exchange in buffered aqueous solution (pH 7.4, 37 degrees C). In contrast, sulofenur itself was stable under these same conditions, even in the presence of GSH and glutathione-S-transferase (GST), thus raising the possibility that bioactivation of sulofenur is necessary for liberation of CPIC. These findings suggest that the generation of this isocyanate in vivo and subsequent carbamoylation of biological macromolecules may play a role in the toxicity and/or antitumor activity of sulofenur and related diarylsulfonylureas.
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Jochheim
authors.forename: Margaret R
authors.initials: MR
authors.lastname: Davis
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Baillie
authors.forename: William J
authors.initials: WJ
authors.lastname: Ehlhardt
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Baillie
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D017953:Isocyanates / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013453:Sulfonylurea Compounds / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D017953:Isocyanates; D013453:Sulfonylurea Compounds; D005978:Glutathione; C452229:chlorophenyl isocyanate; C058179:sulofenur