Effects of prostaglandins on spontaneous apoptosis in gastric mucosal cells.
Prostaglandins have cytoprotective effects on gastric mucosa via the influence of various mechanisms. The purpose of this study is to examine the effects of prostaglandins on maturation-dependent spontaneous apoptosis in gastric mucosal cells in vitro, which mimics the apoptosis of gastric mucosal cells related with a rapid cell turnover rate in vivo. Both prostaglandin E1 and E2 inhibited spontaneous apoptosis in a dose-dependent manner and increased the viability of gastric mucosal cells in culture. A number of antiulcer drugs presently in clinical use were shown to increase the concentrations of prostaglandins in cells. All of the drugs tested clearly inhibited the spontaneous apoptosis in a dose-dependent manner. Based on these results, we propose that the cytoprotective effects of prostaglandins on gastric mucosa in vivo can be partially explained by an increase in the number of gastric mucosal cells present as a result of the inhibition of maturation-dependent spontaneous apoptosis.
Tsutsumi
Shinji
S
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka, Japan.
Haruna
Reiko
R
Tomisato
Wataru
W
Takano
Tatsunori
T
Hoshino
Tatsuya
T
Tsuchiya
Tomofusa
T
Mizushima
Tohru
T
D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation; D015232:Dinoprostone / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects*; D006168:Guinea Pigs; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*
D000897:Anti-Ulcer Agents; D011453:Prostaglandins; D000527:Alprostadil; D015232:Dinoprostone