title: Cortical focus drives widespread corticothalamic networks during spontaneous absence seizures in rats.
abstract: Absence seizures are the most pure form of generalized epilepsy. They are characterized in the electroencephalogram by widespread bilaterally synchronous spike-wave discharges (SWDs), which are the reflections of highly synchronized oscillations in thalamocortical networks. To reveal network mechanisms responsible for the initiation and generalization of the discharges, we studied the interrelationships between multisite cortical and thalamic field potentials recorded during spontaneous SWDs in the freely moving WAG/Rij rat, a genetic model of absence epilepsy. Nonlinear association analysis revealed a consistent cortical "focus" within the peri-oral region of the somatosensory cortex. The SWDs recorded at other cortical sites consistently lagged this focal site, with time delays that increased with electrode distance (corresponding to a mean propagation velocity of 1.4 m/sec). Intra-thalamic relationships were more complex and could not account for the observed cortical propagation pattern. Cortical and thalamic sites interacted bi-directionally, whereas the direction of this coupling could vary throughout one seizure. However, during the first 500 msec, the cortical focus was consistently found to lead the thalamus. These findings argue against the existence of one common subcortical pacemaker for the generation of generalized spike-wave discharges characteristic for absence seizures in the rat. Instead, the results suggest that a cortical focus is the dominant factor in initiating the paroxysmal oscillation within the corticothalamic loops, and that the large-scale synchronization is mediated by ways of an extremely fast intracortical spread of seizure activity. Analogous mechanisms may underlie the pathophysiology of human absence epilepsy.
authors.affiliation: Department of Comparative and Physiological Psychology, Nijmegen Institute of Cognition and Information, University of Nijmegen, 6500 HE Nijmegen, The Netherlands. h.meeren@vumc.nl.
authors.forename: Hanneke K M
authors.initials: HK
authors.lastname: Meeren
authors.forename: Jan Pieter M
authors.initials: JP
authors.lastname: Pijn
authors.forename: Egidius L J M
authors.initials: EL
authors.lastname: Van Luijtelaar
authors.forename: Anton M L
authors.initials: AM
authors.lastname: Coenen
authors.forename: Fernando H
authors.initials: FH
authors.lastname: Lopes da Silva
mesh_terms: D000200:Action Potentials; D000818:Animals; D001683:Biological Clocks; D001931:Brain Mapping; D002540:Cerebral Cortex / Q000503:physiopathology*; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004569:Electroencephalography; D004828:Epilepsies, Partial / Q000150:complications / Q000503:physiopathology*; D004832:Epilepsy, Absence / Q000150:complications / Q000503:physiopathology*; D005071:Evoked Potentials; D005073:Evoked Potentials, Somatosensory; D009415:Nerve Net / Q000503:physiopathology*; D017711:Nonlinear Dynamics; D051381:Rats; D011919:Rats, Inbred Strains; D012815:Signal Processing, Computer-Assisted; D013788:Thalamus / Q000503:physiopathology*; D013997:Time Factors; D014851:Wakefulness