[Apoptosis of endothelial cells in alteration of microvascular permeability in lung during sepsis].
OBJECTIVE
To investigate the apoptosis of endothelial cells in the alteration of microvascular permeability in lung during sepsis.


METHODS
Twenty-four male NIH mice were subjected to cecal ligation and puncture (CLP) or sham operation (SO). The microvascular endothelial cells of the lungs were harvested at 3 hours and 12 hours after operation. The apoptosis of endothelial cells (VIII factor related antigen positive cells) in the lungs was determined by 2-color flow cytometry (FITC-labeled VIII factor related antigen and PI) and Apop Tag) in situ apoptosis detection kit in situ. The apoptosis related gene (bcl-2) was detected by RT-PCR. The microvascular permeability in lung tissue was also examined.


RESULTS
The apoptosis of microvascular endothelial cells was increased significantly at 12 hours after CLP (5.03 +/- 0.92 vs. 3.48 +/- 1.21, P < 0.05). The increased apoptosis was paralleled with the decrease in bcl-2 gene expression. The microvascular permeability in lung tissue was slightly increased at 3 hours after CLP, but significantly increased at 12 hours after CLP (0.106 +/- 0.008 vs 0.047 +/- 0.003, P < 0.01).


CONCLUSIONS
Apoptosis in microvascular endothelial cells seems to be an important reason for the alteration of microvascular permeability.
Wu
R
R
Institute of General Surgery, General Hospital of People's Liberation Army, Beijing 100853, China.
Song
X
X
Xu
Y
Y
Meng
X
X
D000818:Animals; D017209:Apoptosis; D002199:Capillary Permeability; D004847:Epithelial Cells / Q000473:pathology*; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D008168:Lung / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018805:Sepsis / Q000473:pathology / Q000503:physiopathology*