title: IL-10 production in circulating T cells differs between allergen-induced isolated early and dual asthmatic responders.
abstract: BACKGROUND
  IL-10 is an anti-inflammatory cytokine released from various cells, including T cells. The role of IL-10 in asthma pathogenesis remains uncertain. Allergen inhalation by atopic asthmatic subjects results in 2 bronchoconstrictor phenotypes: isolated early response and dual response. Persistence of allergen-induced airway inflammation is a feature of dual responders.
  
  
  OBJECTIVE
  The kinetics of IL-10 production in circulating T cells were investigated to examine a potential role of IL-10 in allergen-induced responses and airway inflammation.
  
  
  METHODS
  Fourteen subjects with mild asthma (7 isolated early and 7 dual responders) were challenged with allergen. PBMCs taken before and 24 hours after allergen challenge were processed for intracellular IL-10 staining with fluorescent-conjugated anti-IL-10 antibody. The frequency of IL-10-producing cells was assessed for CD4(+) and CD8(+) T-cell subsets by using flow cytometry.
  
  
  RESULTS
  Before allergen administration, the frequency of IL-10-producing CD4(+) cells was significantly higher in dual than in isolated early responders. IL-10-producing CD4(+) cells significantly increased after allergen in early responders, whereas IL-10-producing CD4(+) cells significantly decreased in dual responders. Simultaneous assessments of IL-5-producing T cells did not show any differences between each group before or after allergen administration.
  
  
  CONCLUSIONS
  These results suggest that the contrasting profiles of IL-10 production may be associated with the different time course of allergen-induced airway inflammation between allergen-induced early and dual responders.
authors.affiliation: Asthma Research Group, St Joseph's Healthcare and McMaster University, Hamilton, Ontario, Canada.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Gail M
authors.initials: GM
authors.lastname: Gauvreau
authors.forename: Tracy
authors.initials: T
authors.lastname: Rerecich
authors.forename: Richard M
authors.initials: RM
authors.lastname: Watson
authors.forename: Lorna J
authors.initials: LJ
authors.lastname: Wood
authors.forename: Paul M
authors.initials: PM
authors.lastname: O'Byrne
mesh_terms: D000280:Administration, Inhalation; D000328:Adult; D000485:Allergens / Q000009:adverse effects / Q000276:immunology*; D001249:Asthma / Q000209:etiology / Q000276:immunology*; D001985:Bronchial Provocation Tests; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D013183:Sputum / Q000166:cytology / Q000276:immunology
chemical_list: D000485:Allergens; D016753:Interleukin-10