Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography.
High-speed counter-current chromatography was successfully used for the isolation and purification of secoisolariciresinol diglucoside, a bioactive lignan from flaxseed (Linum usitatissimum L.). The solvent system consisted of tert.-butylmethyl ether-n-butanol-acetonitrile-water (1:3:1:5). The purity and identity of the isolated compound was checked by high-performance liquid chromatography analysis in combination with mass spectrometry and NMR measurements.
Degenhardt
Andreas
A
Institute of Food Chemistry, Technical University of Braunschweig, Germany.
Habben
Saskia
S
Winterhalter
Peter
P
D002072:Butylene Glycols / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D019597:Flax / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D002072:Butylene Glycols; D005960:Glucosides; C090142:secoisolariciresinol diglucoside