Comparative evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syncytial virus (BRSV) in dairy cattle.
Seroprevalence of bovine respiratory syncytial virus (BRSV) infection in both exotic and crossbred cattle were described. A baculovirus expressed recombinant purified nucleocapsid (N) protein was used in indirect and sandwich ELISA for screening of 499 bovine sera samples from all over the state for the presence of BRSV antibodies. The seroprevalence rate of BRSV was found to be 46.09% through indirect ELISA while it would found to be 65.33% by sandwich ELISA. The result also indicated that exotic breeds were more susceptible to BRSV infection compared to crossbred cattle. A comprehensive analysis on susceptibility to BRSV as regards to various factors like age and sex was also summarized.
Hazari
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Department of Bacteriology and Virology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Orissa, India. sidharthah@yahoo.com
Panda
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Kar
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Das
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D007194:India / Q000453:epidemiology; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*; D016036:Seroepidemiologic Studies
D000914:Antibodies, Viral