[Experimental condition for WST-1 assay of skin viability].
OBJECTIVE
To investigate a new tetrazoliium salt assay (WST-1) of detecting tissue and cells of preserved skin.


METHODS
With WST-1, viability of porcine and human skin split were assessed. The experimental condition such as dosage of agents, thickness of skin split, time of reaction, area of skin split and weight of skin split were determined.


RESULTS
Optimal experimental condition of skin split should be 10 mm diameter and 0.3 mm thick, incubation for 3 hours at 37 degrees C, and reagent dosage of 20 microl.


CONCLUSIONS
As a reagent for detecting cell viability, WST-1 is successfully applied to the assessment of skin viability.
Jia
X
X
Burn Institute, 304th Hospital, People's Liberation Army, Beijing 100037.
Yang
H
H
Liss
K
K
McGann
L E
LE
Tredget
E E
EE
D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D012867:Skin / Q000737:chemistry*; D012869:Skin Absorption; D013524:Surgical Flaps / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature; D013778:Tetrazolium Salts / Q000032:analysis*; D014022:Tissue Survival
C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D013778:Tetrazolium Salts