title: [Important bio-thermal physical problems and latest advancement in laser cell engineering].
abstract: The recently emerging technique of laser microsurgery (optical tweezers, optical scissors, etc.) is providing a new precise, sterile method for the cell engineering practices such as introduction of external gene into an object cell, cell-fusion, and trapping or transportation of microscopic objects (cells or chromosomes etc.). The thermal effects thus induced usually proved to be critical factors for successful operation of this method. In order to meet the requirement for the rapid development in this territory, some important bio-thermal physical problems and the corresponding research subjects in this area were comprehensively summarized. Difficulties and critical issues were discussed. The latest advancement of the laser cell engineering was also described. This review is attempted to bridge up the gap between bioengineering and thermal science fields and then to enhance the rapid progress of laser microsurgery.
authors.affiliation: Cryogenic Laboratory, Chinese Academy of Sciences, Beijing, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D002459:Cell Fusion / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D004735:Energy Transfer; D005818:Genetic Engineering / Q000295:instrumentation / Q000379:methods; D006358:Hot Temperature; D053685:Laser Therapy / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*