title: High prevalence of autoantibodies against the nuclear high mobility group (HMG) protein SSRP1 in sera from patients with systemic lupus erythematosus, but not other rheumatic diseases.
abstract: OBJECTIVE
  To evaluate the presence of autoantibodies to the high mobility group (HMG) structure specific recognition protein I (SSRP1) in sera from patients with systemic lupus erythematosus (SLE) or other rheumatic diseases.
  
  
  METHODS
  Antibodies to SSRP1(anti-SSRP1) were measured in sera from patients with SLE, Sjogren's syndrome (SS), ulcerative colitis (UC), systemic sclerosis (SSc), rheumatoid arthritis (RA), and sera from healthy individuals by both an enzyme-linked immunoassay (ELISA) and Western blotting (WB) using the recombinant SSRP1 N-terminus as antigen.
  
  
  RESULTS
  We found 28.8% of the sera from patients with SLE contained anti-SSRPI by both ELISA and WB assay, compared to 8.3% of the sera from healthy individuals. When the 40 sera from patients with other autoimmune diseases were tested, only 2 sera (5%) from individuals with SS showed a moderate reactivity to SSRPI in both ELISA and WB assays.
  
  
  CONCLUSIONS
  The results show that anti-SSRPI can be identified in sera from patients with SLE, but not with other rheumatic diseases and may thus help the diagnosis of SLE in the presence of appropriate clinical findings.
authors.affiliation: From the Immunogenetics and Experimental Oncology Center, CNR, University of Turin, Italy.
authors.forename: Piera
authors.initials: P
authors.lastname: Santoro
authors.forename: Marco
authors.initials: M
authors.lastname: De Andrea
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Migliaretti
authors.forename: Claudio
authors.initials: C
authors.lastname: Trapani
authors.forename: Santo
authors.initials: S
authors.lastname: Landolfo
authors.forename: Marisa
authors.initials: M
authors.lastname: Gariglio
mesh_terms: D000918:Antibody Specificity / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology*; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000097:blood / Q000276:immunology; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006609:High Mobility Group Proteins / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis / Q000276:immunology*; D017434:Protein Structure, Tertiary / Q000502:physiology; D012595:Scleroderma, Systemic / Q000097:blood / Q000276:immunology; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology; D035602:Transcriptional Elongation Factors
chemical_list: D001323:Autoantibodies; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; C073537:SSRP1 protein, human; D035602:Transcriptional Elongation Factors