title: Maillard reaction induced lactose attachment to bovine beta-lactoglobulin: electrospray ionization and matrix-assisted laser desorption/ionization examination.
abstract: Nonenzymatic attachment of lactose to beta-lactoglobulin (beta-Lg) was investigated under different conditions. Solubilized conditions, dry environment, and a combination of dry and solubilized environments, were examined for their effects on lactosylation. Temperatures ranging from 50 to 65 degrees C and time intervals between 1 h and 4 days were used. Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry were implemented to examine the reaction products. Maximum attachment efficiency occurred at 65 degrees C held for 3 h in dry-way conditions. Incubations held for long periods of time under dry-way conditions suggest possible denaturation. Both ESI and MALDI data suggest beta-Lg removal in the solubilized samples held for long periods of time. A combination of solubilized and dry environments led to very similar mass spectrogram results over time.
authors.affiliation: Department of Food Science and Technology, The Ohio State University, 2015 Fyffe Court, Columbus, Ohio 43210, USA. french.27@osu.edu
authors.forename: S J
authors.initials: SJ
authors.lastname: French
authors.forename: W J
authors.initials: WJ
authors.lastname: Harper
authors.forename: N M
authors.initials: NM
authors.lastname: Kleinholz
authors.forename: R B
authors.initials: RB
authors.lastname: Jones
authors.forename: K B
authors.initials: KB
authors.lastname: Green-Church
mesh_terms: D000818:Animals; D002417:Cattle; D006813:Humidity; D007782:Lactoglobulins / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D015416:Maillard Reaction; D012995:Solubility; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D013997:Time Factors
chemical_list: D007782:Lactoglobulins; D007785:Lactose