Shift toward T lymphocytes with Th1 and Tc1 cytokine-secterion profile in the joints of patients with osteoarthritis.
Osteoarthritis (OA), although a heterogeneous disease, is generally believed by rheumatologists to be primarily a disease generated by biomechanical alterations. In order to determine the role of T cells, the pattern of T lymphocyte cytokines and to characterize the mononuclear cells from both peripheral blood (PB) and synovial fluid (SF) from patients with OA flow cytometry with a panel of monoclonal antibodies recognising CD4, CD8 and intracellular cytokines (IL2, IL4, IL10, gamma-IFN) was employed. The coexpression of CD4 and CD8 markers only on the SF T cells surface after in vitro stimulation by phorbol-miristate acetate and ionomicine, but not on PBL or in vitro unstimulated SFL was noted. The intracellular IFN-gamma was detected in enhanced levels in both CD4+ and CD8+ SF T cells, while the IL2 contents was not different in PB and SF samples. The Th2/Tc2 cytokines (IL4 and IL10) were detected in low amounts in both PBL and SFL. This study shows the role of some T cell populations and cytokines in the generation of joint destruction in osteoarthritis.
Dolganiuc
A
A
Cantacuzino Institute, Bucharest, Romania.
Stăvaru
C
C
Anghel
M
M
Georgescu
E
E
Chichoş
B
B
Olinescu
A
A
D000368:Aged; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002470:Cell Survival; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D015759:Ionomycin; D007719:Knee Joint / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D010003:Osteoarthritis / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives*; D018417:Th1 Cells / Q000276:immunology
D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D015759:Ionomycin; C016658:phorbolol myristate acetate; D013755:Tetradecanoylphorbol Acetate