title: Nef and LTR sequence variation from sequentially derived human immunodeficiency virus type 1 isolates.
abstract: Variation in HIV-1 nef and LTR DNA sequences was assessed longitudinally during disease progression in four HIV-1-infected subjects. Point mutations were found among quasispecies obtained at a single time point in each individual, with increasing diversity with disease progression in two of three patients for whom sufficient data were available for analysis. Deletions and rearrangements were more common in late than early stages of disease. Continued sequence evolution in HIV-1 quasispecies with nef deletions along with coexistence of nef-bearing quasispecies suggest that nef-deleted quasispecies are capable of replication in vivo, possibly complemented by quasispecies lacking such deletions and/or by adaptation to a specialized niche within the patients.
authors.affiliation: Department of Medicine,Washington University, St Louis, Missouri 63110, USA.
authors.forename: T
authors.initials: T
authors.lastname: McNearney
authors.forename: Z
authors.initials: Z
authors.lastname: Hornickova
authors.forename: A
authors.initials: A
authors.lastname: Templeton
authors.forename: A
authors.initials: A
authors.lastname: Birdwell
authors.forename: M
authors.initials: M
authors.lastname: Arens
authors.forename: R
authors.initials: R
authors.lastname: Markham
authors.forename: A
authors.initials: A
authors.lastname: Saah
authors.forename: L
authors.initials: L
authors.lastname: Ratner
mesh_terms: D000595:Amino Acid Sequence; D015693:Gene Products, nef / Q000235:genetics*; D014644:Genetic Variation; D015658:HIV Infections / Q000235:genetics / Q000821:virology*; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus