Do microtubules around the Toxoplasma gondii-containing parasitophorous vacuole in skeletal muscle cells form a barrier for the phagolysosomal fusion?
The intracellular fate of Toxoplasma gondii was studied in primary cultures of skeletal muscle cells (SMC). The labelling of secondary lysosomes with BSA-Au particles showed no phagolysosomal fusion with the vacuole containing the parasite. After internalization of the parasites, the parasitophorous vacuole became involved by closely apposed endoplasmic reticulum (ER) and mitochondria; within 18 h of interaction, microtubules were visualized in association with the parasitophorous vacuole, suggesting that they could form a barrier for the phagolysosomal fusion.
Andrade
E F
EF
Department of Ultrastructure and Cellular Biology, Oswaldo Cruz Institute, FIOCRUZ, RJ, Brazil.
Stumbo
A C
AC
Monteiro-Leal
L H
LH
Carvalho
L
L
Barbosa
H S
HS
D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D008870:Microtubules / Q000469:parasitology* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000469:parasitology* / Q000648:ultrastructure; D010588:Phagosomes / Q000469:parasitology* / Q000648:ultrastructure; D014122:Toxoplasma / Q000502:physiology* / Q000648:ultrastructure; D014124:Toxoplasmosis, Animal / Q000503:physiopathology*; D014617:Vacuoles / Q000469:parasitology* / Q000648:ultrastructure