Modulation of the maitotoxin response by intracellular and extracellular cations.
The aim of the present study was to characterize the role played by intracellular and extracellular calcium and sodium on the maitotoxin (MTX) response in Chinese hamster ovary (CHO) cells. The results presented here indicated that: (1) MTX activates calcium and sodium influx in a concentration-dependent manner; (2) extracellular calcium is required for the sodium influx; (3) removal of the extracellular sodium did not prevent the MTX-induced calcium influx; (4) elevation in the intracellular calcium concentration potentiates the MTX response; and (5) MTX, at the concentrations tested, did not compromise cell viability.
Morales-Tlalpan
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Departamento de Biología Celular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de Mexico, 04510, Mexico, Mexico.
Vaca
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D000465:Algorithms; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space / Q000737:chemistry; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D008387:Marine Toxins / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D035964:Oxocins; D012964:Sodium / Q000378:metabolism; D000095702:Polyether Toxins
D002118:Calcium; D002412:Cations; D007770:L-Lactate Dehydrogenase; D008387:Marine Toxins; D035964:Oxocins; D012964:Sodium; C020394:maitotoxin; D000095702:Polyether Toxins