Preliminary evaluation of mast cells in rats with an experimental fibrosarcoma induced by 3-methylcholanthrene.
The aim of the study was the evaluation of mast cells in experimental fibrosarcoma induced in the rats' skin. Experiments were carried out on 50 male Wistar rats divided into three groups: 1. The cancer was induced in 34 rats' by onefold subcutaneous injection of 0.2 mg 3-methylcholanthrene in 0. 25 ml of olive oil; 2.--8 rats received subcutaneous injection of 0.25 ml olive oil; and 3.--8 rats, no treatment. The tumors developed after 14-35 weeks. The examined tumors had the mass of 1 g to 176 g--mean 15 g. Tissue material was fixed in Carnoy's or Bouin's fluid. Paraffin sections were stained with hematoxylin and eosin and using Azan methods. Mast cells were stained with alcjan blue+saphranin and with toluidine blue in pH 1.5. Immunohistochemical reactions detecting tryptase in mast cells and von Willebrand Factor in endothelial cells were also performed--using specific antibodies (DAKO) and ABC complex. We have found a very significant growth of the quantity of mast cells in the connective tissue of tumours. The distinct increase in immunostaining was found for tryptase in mast cells of the tumor periphery zone, where most areas of strong neoangiogenesis were found.
Sawick
B
B
Department of Histology and Embryology, Medical Academy, Białystok, Poland. sawboghe@amb.ac.bialystok.pl
Kasacka
L
L
Chyczewski
L
L
Sobolewski
K
K
D000818:Animals; D003594:Cytoplasmic Granules / Q000473:pathology; D005354:Fibrosarcoma / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000473:pathology*; D008748:Methylcholanthrene; D009372:Neoplasms, Connective Tissue / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000032:analysis; D053802:Tryptases
D008748:Methylcholanthrene; D012697:Serine Endopeptidases; C509223:Tpsab1 protein, rat; C509221:Tpsb2 protein, rat; D053802:Tryptases