Decrease of the electroacupuncture-induced analgesic effects in nuclear factor-kappa B1 knockout mice.
To investigate the involvement of nuclear factor kappa B1 (NF-kappaB1; p50/p105) in electroacupuncture (EA)-induced analgesia, 2 and 100 Hz EA stimulations were applied at acupoint ST36 (Zusanli) in NF-kappaB1 knockout mice. EA was performed for 30 min and tail-flick latencies (TFLs) were evaluated every 15 min for 1 h. Wild-type mice displayed a 63.3% increase in TFLs compared to baseline after 2 Hz EA, whereas NF-kappaB1+/- mice exhibited a 41.8% increase and NF-kappaB1-/- mice showed only a 3.9% increase of TFLs. The TFLs of 100 Hz EA showed similar trends: a 72.6% increase of TFLs in wild-type, a 38.6% increase in NF-kappaB1+/- and a 9.3% increase in NF-kappaB1-/- mice. The present findings suggest that NF-kappaB1 may play a crucial role in both low and high frequency EA-induced analgesic effects.
Park
Hi-Joon
HJ
Research Group of Pain and Neuroscience, Kyung Hee University, Hoegidong, Dongdaemoongu, Seoul, South Korea.
Lee
Hyang-Sook
HS
Lee
Hye-Jung
HJ
Yoo
Yeong-Min
YM
Lee
Hee Jae
HJ
Kim
Soon Ae
SA
Leem
Kanghyun
K
Kim
Ho-Chul
HC
Seo
Jung-Chul
JC
Kim
Ee-Hwa
EH
Lim
Sabina
S
Chung
Joo-Ho
JH
D000698:Analgesia; D000818:Animals; D015671:Electroacupuncture; D005260:Female; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics / Q000502:physiology*; D010146:Pain / Q000503:physiopathology; D010147:Pain Measurement; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D011930:Reaction Time / Q000502:physiology; D013623:Tail / Q000503:physiopathology
D016328:NF-kappa B; D020033:Protein Isoforms