Effects of S-adenosyl-L-methionine on liver damage in experimental obstructive jaundice.
S-adenosyl-l-methionine (SAMe) is a naturally occurring molecule distributed throughout the body tissues, including the liver. It acts as a methyl group donor and as an enzyme activator in a number of biochemical reactions. Methionine is metabolized in the liver, where it is converted to SAMe by SAMe-synthetase. In patients with liver diseases, these pathways are impaired because of the decreased contents of glutathione, the major abnormality being a reduction in SAMe-synthetase activity. Exogenous SAMe may overcome the results of impaired SAMe-synthetase activities. We conducted this study to evaluate the effect of SAMe administration on liver damage induced by biliary obstruction. Rats with common bile duct ligation exhibited abnormal liver functions, increased lipid peroxide levels, and decreased reduced glutathione contents when compared with the shammed-controls, which indicated that there was oxidative stress in rats with obstructive jaundice; however, SAMe application improved these injuries. There were significant alterations of the levels of amino acid profiles in animals with obstructive jaundice. The ratio between branch chain and aromatic amino acid was depressed, which indicated that the condition of liver was worsening, but SAMe administration improved these alterations significantly. In conclusion, SAMe administration alleviated the liver damage, indicating an important hepatoprotective effect of this methyl donor.
Tsai
S M
SM
Department of Health Public, School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Lee
K T
KT
Tsai
L Y
LY
D000596:Amino Acids / Q000097:blood; D000818:Animals; D002779:Cholestasis / Q000097:blood / Q000188:drug therapy*; D005978:Glutathione / Q000032:analysis; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012436:S-Adenosylmethionine / Q000627:therapeutic use*
D000596:Amino Acids; D012436:S-Adenosylmethionine; D005978:Glutathione