A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae.
A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
Honoré
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Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris, France.
Roche
P W
PW
Grosset
J H
JH
Cole
S T
ST
D000818:Animals; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology*; D007918:Leprosy / Q000175:diagnosis* / Q000188:drug therapy; D051379:Mice; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology*
D007917:Leprostatic Agents; D015345:Oligonucleotide Probes; D012293:Rifampin