title: Rat monoclonal antibodies differentiating between the Epstein-Barr virus nuclear antigens 2A (EBNA2A) and 2B (EBNA2B).
abstract: Rat monoclonal antibodies were produced against the C-terminus of Epstein-Barr virus nuclear antigens 2A (EBNA2A) and 2B (EBNA2B) expressed as bacterial trpE fusion proteins. The initial screening was performed using a soluble bacterial extract containing the fusion proteins. Positive hybridomas were confirmed by immunofluorescence on SF158 (Spodoptera frugiperda) insect cells infected with recombinant baculovirus (Autographa californica nuclear polyhedrosis virus) and expressing the complete EBNA2A or EBNA2B genes. We selected a panel of antibodies which reacted either with both antigens or specifically with EBNA2A or with EBNA2B. The antibodies were extensively characterized using immunoprecipitation, Western blotting, epitope mapping on synthesized peptide segments of EBNA2A, immunocytology, and immunohistology on both cryostat sections and paraffin sections of AIDS-associated primary central nervous system lymphomas.
authors.affiliation: Institut für Immunologie, Hämatologikum, GSF, München, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Kremmer
authors.forename: B R
authors.initials: BR
authors.lastname: Kranz
authors.forename: A
authors.initials: A
authors.lastname: Hille
authors.forename: K
authors.initials: K
authors.lastname: Klein
authors.forename: M
authors.initials: M
authors.lastname: Eulitz
authors.forename: G
authors.initials: G
authors.lastname: Hoffmann-Fezer
authors.forename: W
authors.initials: W
authors.lastname: Feiden
authors.forename: K
authors.initials: K
authors.lastname: Herrmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Delecluse
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: G W
authors.initials: GW
authors.lastname: Bornkamm
authors.forename: N
authors.initials: N
authors.lastname: Mueller-Lantzsch
authors.forename: F A
authors.initials: FA
authors.lastname: Grässert
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D018604:Epitope Mapping; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D006825:Hybridomas; D051381:Rats; D014764:Viral Proteins
chemical_list: D000911:Antibodies, Monoclonal; C402641:EBNA-2 protein, Human herpesvirus 4; D019309:Epstein-Barr Virus Nuclear Antigens; D014764:Viral Proteins