Enhanced expression of nitric oxide synthase in the early stage after increased pulmonary blood flow in rats.
OBJECTIVE
Evidence that vasodilator nitric oxide mediates normal pulmonary vascular tone has led to the hypothesis that endothelial injury induced by congenital heart disease with increased pulmonary blood flow disrupts these regulatory mechanisms and its associated altered vascular reactivity. Therefore, we hypothesized that increased pulmonary blood flow results in altered expression of endothelial nitric oxide synthase (eNOS).


METHODS
We created an arteriovenous shunt in female Wistar (5-week-old) and measured the change of pulmonary blood flow and pressure immediately after and 1 month after the shunt operation. The protein levels of eNOS in the lung tissues of rats were assessed.


RESULTS
The shunt immediately resulted in a significant increase in pulmonary blood flow (16.5 +/- 11.8% , pulmonary artery pressure (2.3 +/- 0.7 mm Hg), and blood O(2) saturation (16.1 +/- 11.8%) in the pulmonary artery. After 4 weeks, there was a significant increase in pulmonary blood flow (30.7 +/- 1.6%), pulmonary artery pressures (4.3 +/- 1.1 mm Hg), and blood O(2) content (43.3 +/- 17.5%). Western blot analysis demonstrated that eNOS protein was increased in the shunt lung 72 h after surgery and recovered to the control level 1 week later.


CONCLUSIONS
This simple shunt model can induce early upregulation of eNOS expression with increased pulmonary blood flow and pulmonary artery pressure in rats.
Chou
Tsai-Fwu
TF
Department of Cardiac surgery, Taipei Municipal Jen-Ai Hospital, Taipei, Taiwan ROC.
Wu
Ming-Shiou
MS
Chien
Chiang-Ting
CT
Yu
Chia-Cherng
CC
Chen
Chau-Fong
CF
D000704:Analysis of Variance; D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D015415:Biomarkers / Q000032:analysis; D001783:Blood Flow Velocity; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D006976:Hypertension, Pulmonary / Q000201:enzymology* / Q000503:physiopathology*; D007150:Immunohistochemistry; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000378:metabolism*; D011336:Probability; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
D015415:Biomarkers; D019001:Nitric Oxide Synthase