Phage-display antibody detection of Chlamydia trachomatis-associated antigens.
A phage-displayed human single-chain Fv antibody library (6.7x10(9) members) was used to select probes specific to components associated with the surface of Chlamydia trachomatis elementary bodies (EBs). Each of 15 antibodies was characterized by ELISA, dot-blot, immunoblot and immunocytochemistry, resulting in the identification of several new chlamydial components associated with the surface of EBs. In addition, six antibodies were specific for host-cell components associated with the surface of EBs. While phage display has been used effectively to produce specific antibodies for purified components, these data show that this technology is suitable for selection of specific probes from complex antigens such as the surface of a microbial pathogen.
Lindquist
Erika A
EA
Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA1.
Marks
James D
JD
Departments of Anesthesia and Pharmaceutical Chemistry2 and the Francis I. Proctor Foundation3, University of California, San Francisco, CA 94143, USA.
Kleba
Betsy J
BJ
Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA1.
Stephens
Richard S
RS
Departments of Anesthesia and Pharmaceutical Chemistry2 and the Francis I. Proctor Foundation3, University of California, San Francisco, CA 94143, USA.|Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360, USA1.
D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics; D019151:Peptide Library; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007128:Immunoglobulin Fragments; D019151:Peptide Library; C055966:immunoglobulin Fv