title: Small-scale batch crystallization of proteins revisited: an underutilized way to grow large protein crystals.
abstract: Growth of high-quality crystals is a major obstacle in many structural investigations. In recent years, the techniques for screening crystals have improved dramatically, whereas the methods for obtaining large crystals have progressed more slowly. This is an important issue since, although many structures can be solved from small crystals with synchrotron radiation, it is far easier to solve and refine structures when strong data is recorded from large crystals. In an effort to improve the size of crystals, a strategy for a small-scale batch method has been developed that in many cases yields far larger crystals than attainable by vapor diffusion.
authors.affiliation: Department of Biochemistry, University of Wisconsin, Madison, Madison, WI 53705, USA. ivan_rayment@biochem.wisc.edu
authors.forename: Ivan
authors.initials: I
authors.lastname: Rayment
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization; D003461:Crystallography / Q000379:methods*; D004058:Diffusion; D008970:Molecular Weight; D009821:Oils; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D009821:Oils; D011506:Proteins