Charged amino acids conserved in the aromatic acid/H+ symporter family of permeases are required for 4-hydroxybenzoate transport by PcaK from Pseudomonas putida.
Charged amino acids in the predicted transmembrane portion of PcaK, a permease from Pseudomonas putida that transports 4-hydroxybenzoate (4-HBA), were required for 4-HBA transport, and they were also required for P. putida to have a chemotactic response to 4-HBA. An essential amino acid motif (DGXD) containing aspartate residues is located in the first transmembrane segment of PcaK and is conserved in the aromatic acid/H+ symporter family of the major facilitator superfamily of transporters.
Ditty
Jayna L
JL
Department of Microbiology, The University of Iowa, Iowa City, IA 52242, USA.
Harwood
Caroline S
CS
D000595:Amino Acid Sequence; D024322:Amino Acids, Aromatic / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002633:Chemotaxis; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010226:Parabens / Q000378:metabolism*; D016958:Pseudomonas putida / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D027981:Symporters / Q000145:classification
D024322:Amino Acids, Aromatic; D001426:Bacterial Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D010226:Parabens; C090106:PcaK protein, Pseudomonas; D027981:Symporters; C038193:4-hydroxybenzoic acid