title: [Single nucleotide polymorphism analysis in chicken insulin-like growth factor-II gene and its associations with growth and carcass traits].
abstract: In this experiment, F2 chicken derived from Broilers crossing with Silky was used to study the effect of insulin-like growth factor-II gene on growth and carcass traits. The partial gene was amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) was detected by the technique of restriction fragment length polymorphism (RFLP), and then confirmed by DNA sequencing. The mutation was found in the exon-2 of the gene, and can be clarified by cutting of restriction enzyme Aci-I. The result of least square analysis showed the gene was significantly related with growth and carcass traits. It implied that the insulin-like growth factor-II gene could be a genetic locus or linked to a major gene affecting greatly the growth and carcass traits in chicken.
authors.affiliation: National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
authors.forename: Bing-Xue
authors.initials: BX
authors.lastname: Yan
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Deng
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Hu
authors.forename: Zhao-Liang
authors.initials: ZL
authors.lastname: Liu
authors.forename: Xing-Bo
authors.initials: XB
authors.lastname: Zhao
authors.forename: Zheng-Xing
authors.initials: ZX
authors.lastname: Lian
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Wu
mesh_terms: D000818:Animals; D002645:Chickens; D008040:Genetic Linkage; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000502:physiology; D016018:Least-Squares Analysis; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable
chemical_list: D007335:Insulin-Like Growth Factor II