Measuring proteins on microarrays.
A prerequisite of proteomics is the ability to quantify many selected proteins simultaneously. Immunoassays on microarrays are an attractive solution, as equipment and antibodies are available and assays are simple, scalable and reproducible. Recently, considerable progress has been made in this area as evidenced by increased sensitivity and coverage (degree of multiplexing). Routine use of antibody microarrays in research and diagnostic settings will require increased availability of binding reagents, novel signal amplification procedures, inexpensive and robust platforms for microarray production and detection, and turn-key systems for running high-throughput assays.
Schweitzer
Barry
B
Molecular Staging Inc., 300 George Street, Suite 701, New Haven, CT 06511, USA. barrys@molecularstaging.com
Kingsmore
Stephen F
SF
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D019151:Peptide Library; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D019151:Peptide Library; D011506:Proteins