[A study on expression of CD44V6 and PCNA in squamous cell carcinomas].
OBJECTIVE
To investigate the expression of cluster of differentiation 44 variant 6 (CD44V6) and proliferating cell nuclear antigen (PCNA) in squamous cell carcinomas (SCC) in eyes.


METHODS
Streptavidinbiotin peroxidase complex (SABC) immunohistochemical method was used to study the expression of CD44V6 and PCNA in 35 cases with squamous cell carcinoma in eyes, 20 cases with papilloma, 11 cases with normal eyelid tissue.


RESULTS
The CD44V6 positive rate was 62.9% (22/35) in squamous cell carcinomas in eyes, 15.0% (3/20) in papillomas, none in 11 cases with normal eyelid tissue. The positive expression rate of CD44V6 in the malignant group was significantly higher than that in the benign and control group, and the positive expression rate of CD44V6 in cases with metastasis was significantly higher than that without metastasis. PCNA labeling index (PI) in the CD44V6 positive expression of tumors was significantly higher than that in the CD44V6 negative expression of tumors (t = 20.21, P < 0.01).


CONCLUSIONS
The over-expression of CD44V6 is correlated with the progress and metastasis of SCC. CD44V6 protein expression is correlated positively with PCNA expression, and the evaluation of both CD44V6 and PCNA is useful for predicting the prognosis.
Niu
Y
Y
Department of Ophthalmology, Affiliated Hospital, Qingdao Medical College, Qingdao 266003, China.
Liu
F
F
Wang
H
H
D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism*; D018450:Disease Progression; D005134:Eye Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis*; D007150:Immunohistochemistry; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010212:Papilloma / Q000175:diagnosis / Q000378:metabolism; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*
D014408:Biomarkers, Tumor; C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D009363:Neoplasm Proteins; D018809:Proliferating Cell Nuclear Antigen