[Upregulation of costimulatory adhesion molecule (CD80) in rat kidney with ischemia/reperfusion injury].
OBJECTIVE
Ischemia/reperfusion injury associated with organ transplantation affects early graft function and acute rejection. Recently, it has been suggested that ischemic insult increases the immunogenicity of the organ. The purpose of this study was to determine the expression of a cell adhesion molecule, CD80, which plays an important role in the costimulatory pathway for full T-cell activation in organ transplantation.


METHODS
Wistar male rats weighing 150-180 g were divided into control group and ischemia groups with temporary clamping of the left renal artery for 30, 60 and 180 minutes. The left kidneys obtained at 1 hour, 1, 3, 7 and 14 days after reperfusion were subjected to total RNA extraction followed by reverse transcriptase-PCR (RT-PCR), fluorescence immunostaining and histological staining.


RESULTS
The expression of CD80 by RT-PCR was not observed in the control group, but was observed in the ischemia groups from 1 to 14 days after reperfusion. Fluorescence immunostaining of CD80 was positive in the ischemia groups, but not in the control group. CD80 expression was detected mainly in the endothelium of the glomeruli and the peritubular vessels.


CONCLUSIONS
These results indicate that CD80 is upregulated by ischemic insult, and that the costimulatory pathway may be enhanced by ischemia/reperfusion injury without alloantigens.
Suzuki
Akira
A
Department of Urology, Akita University School of Medicine, Akita, Japan.
Satoh
Shigeru
S
Tsuchiya
Norihiko
N
Kato
Tetsuro
T
Sato
Mitsuru
M
Senoo
Haruki
H
D000818:Animals; D018122:B7-1 Antigen / Q000096:biosynthesis*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D004727:Endothelium / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D015854:Up-Regulation
D018122:B7-1 Antigen; D015815:Cell Adhesion Molecules