Targeting, insertion, and localization of Escherichia coli YidC.
YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon. Little is known about the biogenesis and structural and functional properties of YidC, itself a polytopic IMP. Here we analyze the targeting and membrane integration of YidC using in vivo and in vitro approaches. The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxa1p. In addition, YidC is shown to be relatively abundant compared with other components involved in IMP assembly and is predominantly localized at the poles of the cell.
Urbanus
Malene L
ML
Department of Microbiology, Institute for Molecular Biological Sciences, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands.
Fröderberg
Linda
L
Drew
David
D
Björk
Petra
P
de Gier
Jan-Willem L
JW
Brunner
Josef
J
Oudega
Bauke
B
Luirink
Joen
J
D001426:Bacterial Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D011485:Protein Binding; D000069816:SEC Translocation Channels
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; C412443:YIDC protein, E coli