title: [Effect of protein expression on antisense c-myc and PCNA for vascular smooth muscle cells].
abstract: OBJECTIVE
  To study the methods of inhibiting proliferation of vascular smooth muscle cells (VSMCs), we observed the change of protein expression after using antisense c-myc, PCNA for VSMCs.
  
  
  METHODS
  The sense, antisense, and mismatched oligomers for c-myc or PCNA were individually delivered to the cultured VSMCs. Using Western blot, immunohistochemistry assay and image analysis system, we observed the change of c-myc and PCNA protein expression in the VSMCs.
  
  
  RESULTS
  Antisense c-myc, PCNA at concentration of 10 micromol/L can successfully inhibit the expression of c-myc, PCNA protein in the VSMCs (P < 0.05), but the sense and the mismatched do not have this effect (P > 0.05).
  
  
  CONCLUSIONS
  The data suggested that the antisense c-myc, PCNA could attenuate the protein expression of c-myc, PCNA, and it is an effective method for the inhibition of VSMCs hyperplasia.
authors.affiliation: Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
authors.forename: J
authors.initials: J
authors.lastname: Bian
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Qian
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D015153:Blotting, Western; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D016376:Oligonucleotides, Antisense; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc