Monoamine oxidase inhibitory coumarin from Zanthoxylum schinifolium.
A methanol extract of Zanthoxylum schinifolium stems at a concentration of 250 microg/ml showed potent inhibitory activity against monoamine oxidase (MAO) in a mouse brain. Activity-guided separation and purification of the extract yielded lacinartin (1) as an active coumarin compound. Lacinartin showed significant inhibitory effects on MAO in a dose-dependent manner. The IC(50) value on MAO activity was 9.2 microM. The MAO-A (IC(50) value, 5.7 microM) sensitivity to lacinartin was greater than that of MAO-B (IC(50) value, 28.6 microM). An enzyme kinetic study revealed that lacinartin inhibited MAO activity by a non-competitive mode.
Jo
Young Su
YS
College of Pharmacy, Chungnam National University, Daejeon, Korea.
Huong
Dang Thi Lan
DT
Bae
KiHwan
K
Lee
Myung Koo
MK
Kim
Young Ho
YH
D000818:Animals; D001921:Brain / Q000201:enzymology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008297:Male; D051379:Mice; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027821:Rutaceae
D003374:Coumarins; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; C452988:lacinartin; C030123:coumarin; D008995:Monoamine Oxidase