Improvement of the electro-transformation efficiency of facultatively alkaliphilic Bacillus pseudofirmus OF4 by high osmolarity and glycine treatment.
An electro-transformation method was established for facultatively alkaliphilic Bacillus pseudofirmus OF4. A high osmolarity electroporation medium with a high electric field strength was effective. Transformation efficiency improved 110-fold in glycine-treated cells compared to non-glycine-treated cells. Under optimum conditions, the transformation efficiency was 1.69 x 10(6) transformants per microg of pUB110.
Ito
M
M
Faculty of Life Sciences, Toyo University, Oura-gun, Gunma, Japan. ito@itakura.toyo.ac.jp
Nagane
M
M
D001407:Bacillus / Q000378:metabolism*; D003470:Culture Media; D018274:Electroporation; D005998:Glycine / Q000378:metabolism*; D009994:Osmolar Concentration
D003470:Culture Media; D005998:Glycine