title: Comparative analysis of sequences expressed during the liquid-cultured mycelia and fruit body stages of Pleurotus ostreatus.
abstract: To characterize genes involved in fruit body development, two complementary DNA (cDNA) libraries were constructed from RNA isolated from liquid-cultured mycelia and fruit bodies of Pleurotus ostreatus. Using single-pass sequencing of cDNA clones, 952 and 1069 expressed sequence tags (ESTs) were generated from liquid-cultured mycelia and fruit body cDNA library, respectively. A BLASTX search revealed that 390 of the liquid-cultured mycelia ESTs (41%) and 531 of the fruit body ESTs (50%) showed significant similarity to protein sequences described in the nonredudant database (E values < or =1 x 10(-5)). When liquid-cultured mycelia and fruit body ESTs were compared by the SeqMan II program, among the total of 2021 ESTs, 1256 ESTs were unigenes, and 66 unigenes (5.3%) were commonly expressed during both stages. The functional catalogs of the ESTs were made by comparison with functionally identified Saccharomyces cerevisiae genes. Liquid-cultured mycelium ESTs were compared with fruit body ESTs and changes of the expressed genes during fruit body development were analyzed.
authors.affiliation: Applied Microbiology Division, Cytogenetics Division, National Institute of Agricultural Science and Technology, 249 Seodun-dong, Suwon, 441-707, South Korea.
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Beom-Gi
authors.initials: BG
authors.lastname: Kim
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Jin-Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Doh-Won
authors.initials: DW
authors.lastname: Yun
authors.forename: Jang-Ho
authors.initials: JH
authors.lastname: Hahn
authors.forename: Gyu-Hyun
authors.initials: GH
authors.lastname: Kim
authors.forename: Kang-Hyo
authors.initials: KH
authors.lastname: Lee
authors.forename: Dong-Sang
authors.initials: DS
authors.lastname: Suh
authors.forename: Suk-Tae
authors.initials: ST
authors.lastname: Kwon
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Young-Bok
authors.initials: YB
authors.lastname: Yoo
mesh_terms: D003470:Culture Media; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags; D015723:Gene Library; D025282:Mycelium / Q000235:genetics; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development; D012331:RNA, Fungal / Q000737:chemistry; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D018076:DNA, Complementary; D012331:RNA, Fungal