title: Expression of Notch ligands, Jagged1, 2 and Delta1 in antigen presenting cells in mice.
abstract: Notch1 is indispensable for T cell development. It is anticipated that Notch1 and other Notch receptors expressed on the surface of thymic T cell precursors are activated by ligands present on environmental cells, including antigen presenting cells (APCs), and involved in positive and negative selections. Notch receptors on peripheral T cells may also be activated by ligands on APCs. Here, we examined the expression pattern of three Notch ligands, Jagged1, 2 and Delta1 in APCs by an immunofluorescence cell staining method and a reverse transcriptase-polymerase chain reaction (RT-PCR) method. Peritoneal macrophages were strongly positive for Jagged1 staining. In contrast, macrophages separated from spleen and dendritic cells (DCs) separated from spleen and thymus showed positive staining for all the three ligands at a similar intensity. An analysis by RT-PCR revealed that peritoneal and splenic macrophages and splenic and thymic DCs, show a distinct pattern in Notch ligand expression. These findings may represent that expression of various Notch ligands in APCs has a physiological relevance in each organ.
authors.affiliation: Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Etsuko
authors.initials: E
authors.lastname: Yamaguchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Keiki
authors.initials: K
authors.lastname: Kumano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kunisato
authors.forename: Tokiharu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hisamaru
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000276:immunology; D002469:Cell Separation; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007136:Immunoglobulins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008024:Ligands; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000276:immunology; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins
chemical_list: D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D007136:Immunoglobulins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; C490503:Jag2 protein, mouse; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008024:Ligands; D008565:Membrane Proteins; D011506:Proteins; D018121:Receptors, Cytokine; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; C410591:Tslpr protein, mouse