Quantitative molecular characterization of bovine vitreous and lens with non-invasive dynamic light scattering.
The non-invasive technique of dynamic light scattering (DLS) was used to quantitatively characterize vitreous and lens structure on a molecular level by measuring the sizes of the predominant particles and mapping the three-dimensional topographic distribution of these structural macromolecules in three spatial dimensions. The results of DLS measurements in five fresh adult bovine eyes were compared to DLS measurements in model solutions of hyaluronan (HA) and collagen (Coll). In the bovine eyes DLS measurements were obtained from excised samples of gel and liquid vitreous and compared to the model solutions. Measurements in whole vitreous were obtained at multiple points posterior to the lens to generate a three-dimensional 'map' of molecular structure. The macromolecule distribution in bovine lens was similarly characterized.In each bovine vitreous (Bo Vit) specimen, DLS predominantly detected two distinct particles, which differed in diffusion properties and hence size. Comparisons with model vitreous solutions demonstrated that these most likely corresponded to the Coll and HA components of vitreous. Three-dimensional mapping of Bo Vit found heterogeneity throughout the vitreous body, with different particle size distributions for Coll and HA at different loci. In contrast, the three-dimensional distribution of lens macromolecules was more homogeneous. Thus, the non-invasive DLS technique can quantitate the average sizes of vitreous and lens macromolecules and map their three-dimensional distribution. This method to assess quantitatively the macromolecular structure of vitreous and lens should be useful for clinical as well as experimental applications in health and disease.
Ansari
R R
RR
NASA Glenn Research Center, Cleveland, OH, USA.
Suh
K I
KI
Dunker
S
S
Kitaya
N
N
Sebag
J
J
D000818:Animals; D002417:Cattle; D003094:Collagen; D006820:Hyaluronic Acid; D007908:Lens, Crystalline / Q000648:ultrastructure*; D008027:Light; D008958:Models, Molecular; D010316:Particle Size; D012542:Scattering, Radiation; D014822:Vitreous Body / Q000648:ultrastructure*
D006820:Hyaluronic Acid; D003094:Collagen