title: The effect of high salt concentration on detection of serum immune complexes and autoantibodies to C1q in patients with systemic lupus erythematosus.
abstract: OBJECTIVE
  To compare the binding of immune complexes and anti-Clq in systemic lupus erythematosus (SLE) sera to Clq and to the purified collagen-like region of Clq (CLR) in high and physiologic NaCl concentrations.
  
  
  METHODS
  Sera from patients with SLE were tested for binding of IgG to Clq and to CLR in physiologic (0.15 M NaCl) or high (1.0 M NaCl) salt concentration. Sera were ultracentrifuged to separate monomeric IgG and high molecular weight IgG (immune complexes), and fractions were tested for binding to C1q and to CLR in the presence of physiologic or high salt concentrations.
  
  
  RESULTS
  Decrease in binding of immune complexes to Clq in the presence of high salt ranged from 0 to 96.8%, with a median of 48.4%. Decrease of the binding of monomeric IgG to C1q in the presence of high salt ranged from 2.2 to 74.3%, with a median of 46.2%. Binding of monomeric autoantibodies to CLR also was decreased by high salt. Nevertheless, anti-Clq measured by binding to CLR in physiologic salt correlated highly with the binding to Clq in both physiologic (r = 0.978) and high salt (r = 0.983).
  
  
  CONCLUSIONS
  Clq-binding of immune complexes in SLE sera is not uniformly abrogated by high salt and binding of autoantibodies to CLR is variably reduced by high salt. Binding of IgG to Clq in high salt correlates with but is not equivalent to quantifying these autoantibodies by binding to CLR.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195-7110, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Kohro-Kawata
authors.forename: Mark H
authors.initials: MH
authors.lastname: Wener
authors.forename: Mart
authors.initials: M
authors.lastname: Mannik
mesh_terms: D000936:Antigen-Antibody Complex / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015922:Complement C1q / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000175:diagnosis / Q000276:immunology*; D011485:Protein Binding / Q000187:drug effects / Q000276:immunology; D012965:Sodium Chloride
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007074:Immunoglobulin G; D012965:Sodium Chloride; D015922:Complement C1q