The glucose transport facilitator GLUT8 is predominantly associated with the acrosomal region of mature spermatozoa.
The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides with the appearance of mature spermatozoa. Furthermore, immunohistochemistry with antiserum against the C-terminus of GLUT8 indicated that the protein was associated with spermatozoa within the seminiferous and the epididymal tubules. The GLUT8 immunoreactivity was detected within the head of mouse and human spermatozoa in the acrosomal region, and appeared to be located at the plasma membrane as well as within the cells. This specific expression and localization of GLUT8 suggests that the transport facilitator plays a major role in the fuel supply of mature spermatozoa, and that it is a potential target for inhibition of sperm cell function.
Schürmann
Annette
A
Institut of Pharmacology and Toxicology, Medical Faculty, Tecnical University of Aachen, Germany. aschuermann@ukaachen.de
Axer
Hubertus
H
Scheepers
Andrea
A
Doege
Holger
H
Joost
Hans-Georg
HG
D000177:Acrosome / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative; D006801:Humans; D008297:Male; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012671:Seminiferous Tubules / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013997:Time Factors
D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C405325:SLC2A8 protein, human; C498628:Slc2a8 protein, mouse