[Adaptation of microtitration plate methods for quantification of the activity of esterases and glutathione-s-transferase in Aedes aegypti].
The detection techniques for the activity of non-specific esterases and glutathione-s-transferase in Culex quinquefasciatus were modified to detect such enzymes in Aedes aegypti(L). The optimal concentration values of substrate (saturating concentration) and the optimum reading time for reaction were determined for each enzyme by using 4 Aedes aegypti strains: one from Cuba 2 from Venezuela and one susceptible reference strain. The frequency of non-specific esterases turned out to be 0.76 in MIRANDA 0.42 in ARAGUA and 1 in SANTIAGO DE CUBA in which the highest frequency value of this mechanisms was reached. The frequency of glutathione-s-transferase mechanism was 0.45 in ARAGUA 0.043 in MIRANDA and 1 in SANTIAGO DE CUBA. For the first time in Cuba a set of biochemical techniques was available for detecting the resistance mechanisms of Aedes aegypti which made it possible to create sound foundations for the easy rapid detection of the resistance of this species the main dengue vector in the Americas.
Rodríguez
M M
MM
ciipk@ipk.sld.cu
Bisset
J A
JA
Molina
D
D
Díaz
C
C
Soca
L A
LA
D000330:Aedes / Q000201:enzymology*; D000818:Animals; D004950:Esterases / Q000032:analysis / Q000378:metabolism*; D005982:Glutathione Transferase / Q000032:analysis / Q000378:metabolism*; D017186:Titrimetry / Q000379:methods*
D005982:Glutathione Transferase; D004950:Esterases