Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells.
The Golgi apparatus is partitioned during mitosis in animal cells by a process of fragmentation, dispersal, and reassembly in each daughter cell. We fractionated the Golgi apparatus in vivo using the drug brefeldin A or a dominant-negative mutant of the Sar1p protein. After these treatments, Golgi enzymes moved back to the endoplasmic reticulum, leaving behind a matrix of Golgi structural proteins. Under these conditions, cells still entered and exited mitosis normally, and their Golgi matrix partitioned in a manner very similar to that of the complete organelle. Thus, the matrix may be the partitioning unit of the Golgi apparatus and may carry the Golgi enzyme-containing membranes into the daughter cells.
Seemann
Joachim
J
Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.
Pypaert
Marc
M
Taguchi
Tomohiko
T
Malsam
Jorg
J
Warren
Graham
G
D000705:Anaphase; D000818:Animals; D001324:Autoantigens; D020126:Brefeldin A / Q000494:pharmacology; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008361:Mannosidases / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008677:Metaphase; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008938:Mitosis; D020559:Monomeric GTP-Binding Proteins / Q000494:pharmacology; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D019704:Protein Disulfide-Isomerases / Q000378:metabolism; D051381:Rats; D029701:Saccharomyces cerevisiae Proteins; D013692:Telophase; D033921:Vesicular Transport Proteins
D001324:Autoantigens; C108705:GORASP1 protein, human; D000077614:Golgi Matrix Proteins; C097741:Golgin subfamily A member 2; C491577:Gorasp1 protein, rat; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D020126:Brefeldin A; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae; D019704:Protein Disulfide-Isomerases