Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization.
We have applied the method of genomic microarray to investigate amplification of oncogenes throughout the genome of nasopharyngeal carcinoma (NPC). Array based comparative genomic hybridization (array CGH) allows simultaneous examination of 58 oncogenes commonly amplified in various human cancers. In the present study, we have examined 15 NPC samples including five cell lines, two xenografts and eight primary tumours with array CGH to reveal the particular oncogenes associated with this cancer. This is the first genome wide survey of multiple oncogene amplifications involved in the development of NPC. Non-random gene amplifications were identified for the first time in NPC on MYCL1 in 1p34.3 and on TERC and PIK3CA at 3q26.3. Other high level amplified oncogenes included NRAS, RAF1, MYB, EGFR, FGF4, EMS1, and D17S167. Highest frequencies of gain of novel oncogenes were detected on MYCL1 (66.7%), TERC (46.7%), ESR (46.7%), PIK3CA (40%), LAMC2 (33.3%), and CSE1L (33.3%).
Hui
Angela Bik-Yu
AB
Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T., Hong Kong, P.R. China. b677750@mailserv.cuhk.edu.hk
Lo
Kwok-Wai
KW
Teo
Peter M L
PM
To
Ka-Fai
KF
Huang
Dolly P
DP
D002277:Carcinoma / Q000235:genetics*; D002874:Chromosome Mapping; D015536:Down-Regulation; D005821:Genetic Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes / Q000235:genetics*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation