A compact view of isochores in the draft human genome sequence.
Prior to genome sequencing, information on base composition (GC level) and its variation in mammalian genomes could be obtained using density gradient ultracentrifugation. Analyses using this approach led to the conclusion that mammalian genomes are organized into mosaics of fairly homogeneous regions, called isochores. We present an initial compositional overview of the chromosomes of the recently available draft human genome sequence, in the form of color-coded moving window plots and corresponding GC level histograms. Results obtained from the draft human genome sequence agree well with those obtained or deduced earlier from CsCl experiments. The draft sequence now permits the visualization of the mosaic organization of the human genome at the DNA sequence level.
Pavlícek
Adam
A
Laboratoire de Génétique Moléculaire, Institut Jacques Monod, 75005 Paris, France.
Paces
Jan
J
Clay
Oliver
O
Bernardi
Giorgio
G
D001482:Base Composition; D002499:Centrifugation, Density Gradient; D002877:Chromosomes, Human / Q000235:genetics; D003198:Computer Simulation; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000235:genetics*; D020862:GC Rich Sequence / Q000235:genetics*; D015894:Genome, Human; D006147:Guanine / Q000032:analysis; D016045:Human Genome Project; D006801:Humans
D006147:Guanine; D003596:Cytosine; D004247:DNA