title: Impairment of vascular regeneration precedes progressive glomerulosclerosis in anti-Thy 1 glomerulonephritis.
abstract: BACKGROUND
  It has been proposed that glomerular hemodynamic changes or glomerular growth response may promote the development of glomerulosclerosis, irrespective of its etiology. Further experimental models are needed to clarify the cellular and molecular mechanisms that lead to progressive glomerulosclerosis with an irreversible course. We designed a model of irreversible glomerulosclerosis, using anti-Thy-1.1 injection followed by uninephrectomy, and examined the role of glomerular endothelial cell responses in the process of progressive sclerotic changes.
  
  
  METHODS
  Rats were injected with anti-Thy-1.1 monoclonal antibody, 1-22-3, and 30 minutes later, unilateral nephrectomy (one-kidney group) or sham operation (two-kidney group) was performed. Rats were sacrificed for histological examination on days 3, 14, 56, and 84 after injection. The density of the glomerular capillary tuft was assessed by immunofluorescent staining for endothelial specific antigens. The mRNA expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), platelet-endothelial cell adhesion molecule-1 (PECAM-1), and vascular endothelial growth factor (VEGF) also was followed up by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Semiquantitative analysis revealed that the capillary density and mRNA expression of PECAM-1, VCAM-1 and VEGF were significantly lower in the one-kidney group compared to the two-kidney group on day 14. On day 84, progressive glomerulosclerotic lesions were found, followed by a decrease of the capillary density in the one-kidney group, while the glomerular architecture recovered to an almost normal state in the two-kidney group.
  
  
  CONCLUSIONS
  Progressive glomerulosclerosis can be induced in the rat by a one shot injection of anti-Thy 1.1 monoclonal antibody followed by unilateral nephrectomy. This model shows that there is a positive association between impairment of vascular regeneration and the development of glomerulosclerosis.
authors.affiliation: Department of Cellular Physiology, Institute of Nephrology, Niigata University School of Medicine, and Gosen Jin-Ai Clinic, Niigata, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Wada
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Morioka
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Oyanagi-Tanaka
authors.forename: Jian
authors.initials: J
authors.lastname: Yao
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Fumitake
authors.initials: F
authors.lastname: Gejyo
authors.forename: Masaaki
authors.initials: M
authors.lastname: Arakawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Oite
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002196:Capillaries / Q000502:physiology; D004195:Disease Models, Animal; D018450:Disease Progression; D015870:Gene Expression; D005921:Glomerulonephritis / Q000473:pathology / Q000503:physiopathology*; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D007678:Kidney Glomerulus / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009392:Nephrectomy; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D011507:Proteinuria / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology; D018800:Thy-1 Antigens / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D018800:Thy-1 Antigens; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1