Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium.
The strictly anaerobic gram-negative bacterium strain Fru22T grows at 50 degrees C in media containing up to 75 g NaCl l(-1). Hexoses and pentoses are fermented to equal molar amounts of acetate, propionate and succinate, and no CO2 is formed. An orange-red pigment similar to flexirrubin is produced during stationary phase upon exposure to light for several days. Cells also produce a surface-active extracellular compound which lowers the surface tension of the medium. This tenside is heat-tolerant up to 70 degrees C and is destroyed by treatment with proteinase K or trypsin, but not by lipase. Comparative 16S rDNA sequence analysis confirmed a phylogenetic affiliation of strain Fru22T to the phylum Bacteroides (Cytophaga/Flavobacterium/Bacteroides), moderately related to the genus Marinilabilia. Therefore, on the basis of phylogenetic, phenotypic and physiological evidence, a new genus, Anaerophaga, is proposed to harbour strain Fru22T (DSM 12881T, OCM 798T) which is described as the type strain of a new species, Anaerophaga thermohalophila gen. nov., sp. nov.
Denger
Karin
K
Warthmann
Rolf
R
Ludwig
Wolfgang
W
Schink
Bernhard
B
D004275:DNA, Ribosomal / Q000032:analysis; D005285:Fermentation; D006089:Gram-Negative Anaerobic Bacteria / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013501:Surface-Active Agents / Q000378:metabolism; D013696:Temperature
D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents; D012965:Sodium Chloride