Simple procedure for rapid identification of Vibrio cholerae from the aquatic environment.
Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae. This technique provided 100% sensitivity and > or =70% specificity.
Choopun
Nipa
N
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt Street, Baltimore, MD 21202.
Louis
Valérie
V
Huq
Anwar
A
Colwell
Rita R
RR
D000818:Animals; D015373:Bacterial Typing Techniques; D003470:Culture Media; D010461:Peptones; D010933:Plankton / Q000382:microbiology; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014734:Vibrio cholerae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D003470:Culture Media; D010461:Peptones