title: [HIV-1 Tat protein induces IL-10 production by human monocytes: implications of the PKC and calcium pathway].
abstract: In asymptomatic patients infected by HIV-1, the level of IL-10, a cytokine with immunosuppressive activity, is associated with the course of HIV infection towards AIDS. We show that HIV-1 Tat, a viral protein secreted by infected cells, induces IL-10 production by human peripheral blood monocytes. The analysis of the signal transduction pathways strongly suggests that the protein kinase C may play an essential role in this induction. Stimulation by Tat induces nuclear translocation of the transcription factor NFkB the activation of which seems to be necessary for IL-10 production. Using microspectrofluorimetry and confocal microscopy, we also show that Tat induces a calcium influx.
authors.affiliation: Laboratoire d'Immuno-Virologie, Université Paul Sabatier 118, route de Narbonne, 31062 Toulouse.
authors.forename: Y
authors.initials: Y
authors.lastname: Bennasser
authors.forename: B
authors.initials: B
authors.lastname: Yamina
authors.forename: X
authors.initials: X
authors.lastname: Contreras
authors.forename: C
authors.initials: C
authors.lastname: Xavier
authors.forename: M
authors.initials: M
authors.lastname: Moreau
authors.forename: M
authors.initials: M
authors.lastname: Marc
authors.forename: C
authors.initials: C
authors.lastname: Le Clerc
authors.forename: L
authors.initials: L
authors.lastname: Catherine
authors.forename: A
authors.initials: A
authors.lastname: Badou
authors.forename: B
authors.initials: B
authors.lastname: Abdallah
authors.forename: E
authors.initials: E
authors.lastname: Bahraoui
mesh_terms: D002118:Calcium / Q000378:metabolism*; D015696:Gene Products, tat / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D016328:NF-kappa B; D054322:tat Gene Products, Human Immunodeficiency Virus; D016753:Interleukin-10; D011493:Protein Kinase C; D002118:Calcium