title: Reversible control of enzymatic transglycosylations in supercritical fluoroform using a lipid-coated beta-D-galactosidase.
abstract: The transgalactosylation rate catalyzed by the lipid-coated beta-d-galactosidase in supercritical fluoroform (scCHF3) can be reversibly controlled by changing temperature or pressure (reflecting polarity changes) without damaging enzymes.
authors.affiliation: Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mori
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Okahata
mesh_terms: D005617:Chlorofluorocarbons, Methane / Q000737:chemistry*; D006031:Glycosylation; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D011312:Pressure; D012995:Solubility; D013696:Temperature; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D005617:Chlorofluorocarbons, Methane; D008055:Lipids; D001616:beta-Galactosidase; C009554:fluoroform