title: Germ-line transformation of the Australian sheep blowfly Lucilia cuprina.
abstract: The Australian sheep blowfly, Lucilia cuprina, is the most important economic insect pest for the sheep industries in Australia and New Zealand. piggyBac-mediated germ-line transformation of L. cuprina was achieved with a helper plasmid that had the Drosophila melanogaster hsp70 promoter controlling expression of the transposase and a piggyBac vector with an EGFP marker gene. Two transformant lines were obtained, at a frequency of approximately 1-2% per fertile G0. One of these lines has a single copy of the transgene, the other most likely has four copies. This is the first report of germ-line transformation of L. cuprina and is an important step towards the generation of engineered strains that would be suitable for male-only release eradication/suppression programmes.
authors.affiliation: Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
authors.forename: J C
authors.initials: JC
authors.lastname: Heinrich
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: R A
authors.initials: RA
authors.lastname: Henry
authors.forename: N
authors.initials: N
authors.lastname: Haack
authors.forename: L
authors.initials: L
authors.lastname: Stringfellow
authors.forename: A C G
authors.initials: AC
authors.lastname: Heath
authors.forename: M J
authors.initials: MJ
authors.lastname: Scott
mesh_terms: D000818:Animals; D001315:Australia; D001483:Base Sequence; D018076:DNA, Complementary; D004175:Diptera / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D017344:Genes, Insect; D005854:Germ Cells; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D012756:Sheep; D014170:Transformation, Genetic
chemical_list: D018076:DNA, Complementary