Preparative chromatography of xylanase using expanded bed adsorption.
Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification.
Silvino
DosSantosEveraldo
D
Biochemical Engineering Laboratory, School of Chemical Engineering, State University of Campinas (UNICAMP), SP, Brazil.
Guirardello
Reginaldo
R
Teixeira
Franco Telma
FT
D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000302:isolation & purification*
D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase