title: Identification of genes essential for growth at high salt concentrations using salt-sensitive mutants of the cyanobacterium Synechocystis sp. strain PCC 6803.
abstract: A collection of 17 salt-sensitive mutants of the cyanobacterium Synechocystis sp. strain PCC 6803 was obtained by random cartridge mutagenesis. The genes coding for proteins essential for growth at high salt concentrations were mapped on the completely known genome sequence of this strain. The two genes coding for enzymes involved in biosynthesis of the osmolyte glucosylglycerol were affected in nine mutants. Two mutants defective in a glycoprotease encoding gene gcp showed a reduced salt resistance. Four genes were identified not previously known to be essential for salt tolerance in cyanobacteria. These genes (slr1799, slr1087, sll1061, and sll1062) code for proteins not yet functionally characterized.
authors.affiliation: Department of Genetics, Moscow State University, School of Biology, 119899 Moscow, Russia.
authors.forename: Inga
authors.initials: I
authors.lastname: Karandashova
authors.forename: Irina
authors.initials: I
authors.lastname: Elanskaya
authors.forename: Kay
authors.initials: K
authors.lastname: Marin
authors.forename: Josef
authors.initials: J
authors.lastname: Vinnemeier
authors.forename: Martin
authors.initials: M
authors.lastname: Hagemann
mesh_terms: D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005960:Glucosides / Q000096:biosynthesis; D016254:Mutagenesis, Insertional; D009154:Mutation; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D005960:Glucosides; C034727:glucosylglycerol; D012965:Sodium Chloride; D010450:Endopeptidases