Profile of genes showing increased expression following dopamine and methamphetamine exposure in an immortalized neuronal cell line.
Methamphetamine (METH) is a drug of abuse with well-described neurodegenerative effects. Some of the METH-induced degenerative manifestations are thought to be due to increased dopamine (DA) release in the cytoplasm of nerve terminals and subsequent extravasation in the synaptic cleft. Using an immortalized neural cell line, we have made use of the comprehensive cDNA array technology in order to compare and contrast the molecular effects of DA and METH. We found that the two compounds do have many similar but also different effects. Since these neural cells produce no DA, these results demonstrate that many of the METH-induced responses attributed to DA might, in fact, be intrinsic to METH itself. More biochemical studies are needed to investigate DA-independent METH deleterious events in the central nervous system.
Cai
N S
NS
Molecular Neuropsychiatry Section, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, 5500 Nathan Shock Drive, Baltimore, MD 21224, USA.
McCoy
M T
MT
Cadet
J L
JL
D000818:Animals; D002461:Cell Line, Transformed; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004298:Dopamine / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D008694:Methamphetamine / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats
D000697:Central Nervous System Stimulants; D008694:Methamphetamine; D004298:Dopamine