The effect of chemical blockade of PKC with Gö6976 and Gö6983 on proliferation and MAPK activity in IL-6-dependent plasmacytoma cells.
Interleukin-6 (IL-6) mediates growth of murine B9 hybridoma/plasmacytoma cells via Ras-dependent mitogen-activated protein kinase (MAPK) pathway. Preincubation of cells with selective protein kinase C (PKC) inhibitors Gö6976 and Gö6983 leads to enhancement of IL-6-induced p44/p42 MAPK activity. The basal p44/p42 MAPK activity is also stimulated in the presence of both inhibitors. On the other hand, Gö6976 completely blocks proliferation, but Gö6983 which does not inhibit PKC(mu) has no effect on the cell growth. These findings suggest that PKC(mu) is required for proliferation and other PKC isoenzymes are involved in regulation of IL-6-dependent growth of B9 cells by negative regulation of the MAPK pathway.
Iankov
Ianko
I
Division of Immunology, Department of Microbiology, Preclinical University Center, Medical University, Zdrave 2 Street, 1431 Sofia, Bulgaria. ianko@ns.medfac.acad.bg
Praskova
Maria
M
Kalenderova
Silvia
S
Tencheva
Zvetanka
Z
Mitov
Ivan
I
Mitev
Vanio
V
D002227:Carbazoles / Q000494:pharmacology*; D003864:Depression, Chemical; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D010954:Plasmacytoma / Q000378:metabolism* / Q000473:pathology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured
D002227:Carbazoles; D004791:Enzyme Inhibitors; D007211:Indoles; D015850:Interleukin-6; C081021:Go 6976; D011493:Protein Kinase C