Production of bioactive triterpenes by Eriobotrya japonica calli.
Callus tissue cultures induced from an axenic leaf of Eriobotrya japonica (Rosaceae) produced triterpenes in large amounts (ca. 50 mg/g dry wt). Nine triterpenes were characterized as ursolic acid, oleanolic acid, 2alpha-hydoxyursolic acid, maslinic acid, tormentic acid, 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid, hyptadienic acid and a mixture of 3-O-cis-p-coumaroyltormentic acid and 3-O-trans-p-coumaroyltormentic acid. The triterpene composition in the callus tissues was noticeably different from that in intact leaves. The contents of tormentic acid with antidiabetic action, and 2alpha, 19alpha-dihydroxy-3-oxo-urs-12-en-28-oic acid with anti-HIV activity, were much larger than those in the intact leaves. All of the triterpenes isolated from the callus tissues showed an inhibitory effect comparable to (-)-epigallocatechin gallate (EGCG) of green tea on the activation of Epstein-Barr virus early antigen (EBV-EA) induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 2alpha, 19alpha-Dihydroxy-3-oxo-urs-12-en-28-oic acid was the most potent inhibitor among them and caused a significant delay of two-stage carcinogenesis on mouse skin.
Taniguchi
Shoko
S
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Imayoshi
Yoko
Y
Kobayashi
Eri
E
Takamatsu
Yoshie
Y
Ito
Hideyuki
H
Hatano
Tsutomu
T
Sakagami
Hiroshi
H
Tokuda
Harukuni
H
Nishino
Hoyoku
H
Sugita
Daigo
D
Shimura
Susumu
S
Yoshida
Takashi
T
D000818:Animals; D000956:Antigens, Viral / Q000187:drug effects; D000970:Antineoplastic Agents / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009062:Mouth Neoplasms / Q000473:pathology; D027824:Rosaceae / Q000378:metabolism*; D012468:Salivary Gland Neoplasms / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D000970:Antineoplastic Agents; C033305:Epstein-Barr virus early antigen; D014315:Triterpenes