title: Steps toward mapping the human vasculature by phage display.
abstract: The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand-receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.
authors.affiliation: Department of Genito-Urinary Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA. warap@notes.mdacc.tmc.edu
authors.forename: Wadih
authors.initials: W
authors.lastname: Arap
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Kolonin
authors.forename: Martin
authors.initials: M
authors.lastname: Trepel
authors.forename: Johanna
authors.initials: J
authors.lastname: Lahdenranta
authors.forename: Marina
authors.initials: M
authors.lastname: Cardó-Vila
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Giordano
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Mintz
authors.forename: Peter U
authors.initials: PU
authors.lastname: Ardelt
authors.forename: Virginia J
authors.initials: VJ
authors.lastname: Yao
authors.forename: Claudia I
authors.initials: CI
authors.lastname: Vidal
authors.forename: Limor
authors.initials: L
authors.lastname: Chen
authors.forename: Anne
authors.initials: A
authors.lastname: Flamm
authors.forename: Heli
authors.initials: H
authors.lastname: Valtanen
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Weavind
authors.forename: Marshall E
authors.initials: ME
authors.lastname: Hicks
authors.forename: Raphael E
authors.initials: RE
authors.lastname: Pollock
authors.forename: Gregory H
authors.initials: GH
authors.lastname: Botz
authors.forename: Corazon D
authors.initials: CD
authors.lastname: Bucana
authors.forename: Erkki
authors.initials: E
authors.lastname: Koivunen
authors.forename: Dolores
authors.initials: D
authors.lastname: Cahill
authors.forename: Patricia
authors.initials: P
authors.lastname: Troncoso
authors.forename: Keith A
authors.initials: KA
authors.lastname: Baggerly
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Pentz
authors.forename: Kim-Anh
authors.initials: KA
authors.lastname: Do
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Logothetis
authors.forename: Renata
authors.initials: R
authors.lastname: Pasqualini
mesh_terms: D001808:Blood Vessels / Q000502:physiology*; D014644:Genetic Variation; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry; D009928:Organ Specificity; D019151:Peptide Library; D015203:Reproducibility of Results; D012984:Software
chemical_list: D009842:Oligopeptides; D019151:Peptide Library