[A preliminary study of pentanucleotide repeat polymorphism of apolipoprotein(a) gene in coronary atherosclerotic heart disease].
OBJECTIVE
This study inquired into the relationship between a pentanucleotide repeats(PNR) polymorphism of the apolipoprotein(a)[apo(a)] gene and coronary atherosclerotic heart disease(CHD) in Chinese Han nationality.


METHODS
PNR polymorphism of the apo(a) gene from 165 cases of CHD and 153 normal individuals were analyzed by polymerase chain reaction(PCR)-denature polyacrylamide gel electrophoresis-silver stain.


RESULTS
The frequencies of (TTTTA)(5/8) genotype (0.188) and (TTTTA)(5) allele (0.115) in CHD group were remarkably higher than those in control group (0.039, 0.026)(P<0.01, P<0.05).


CONCLUSIONS
These findings indicate that the PNR polymorphism of apo(a) is associated with the susceptibility to CHD, which may be involved in the development of CHD.
Hu
Bo
B
Department of Clinical Laboratory, the Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou, Guangdong, 510630 P. R. China. HuBo-Bo@21cn.com
Zhou
Xin
X
Li
Lin
L
Li
Xi
X
D000368:Aged; D001053:Apolipoproteins / Q000235:genetics*; D053241:Apoprotein(a); D044466:Asian People / Q000235:genetics; D003324:Coronary Artery Disease / Q000235:genetics*; D003327:Coronary Disease / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005787:Gene Frequency; D006801:Humans; D017270:Lipoprotein(a) / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D009841:Oligonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D001053:Apolipoproteins; D017270:Lipoprotein(a); D009841:Oligonucleotides; D053241:Apoprotein(a)