Post-transfusion alloimmunization to granulocytes and platelets in Japanese patients as determined by the MPHA method.
The current occurrence of alloimmunization to granulocytes and platelets after blood transfusion is unclear due to the fact that antibody assays are cumbersome. Using the MPHA method with extracted granulocyte and platelet antigens, a randomized, blinded trial was conducted to investigate three types of alloantibodies in 431 Japanese patients receiving leukocyte-depleted blood transfusions prepared with or without our latest leukocyte-reduction filter. The frequency of granulocyte, platelet and HLA class I alloantibodies was 0.44%, 0.44% and 16.74%, respectively, in patients receiving non-filtered products and 0%, 0% and 0.49%, respectively, in patients receiving filtered products. The granulocyte antibody reacted with an antigen approximately of 51 KDa. The platelet-specific alloantibody was associated with GPIIb/IIIa and GPIa/IIa. The important factors affecting alloimmunization were the transfusion dose and the use of unfiltered platelet products.
Zhang
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X
Department of Transfusion Medicine, Kyoto University Hospital, Kyoto University, College of Medicine, Japan. zhangx0@email.uc.edu
Araki
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N
Ito
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K
D000917:Antibody Formation; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D007564:Japan; D008297:Male; D008875:Middle Aged; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D065227:Transfusion Reaction
D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; D019039:Platelet Glycoprotein GPIIb-IIIa Complex