[Determination of ferulic acid absorbed into human serum from oral decoction of rhizoma chuanxiong and pharmacokinetic study of ferulic acid].
OBJECTIVE
To improve the HPLC method for determining ferulic acid(FA) in the human serum and to research FA clinical pharmacokinetics.


METHODS
Serum concentrations of FA were determined by HPLC using methanol-water-acetic acid (40:59.7:0.3, v/v) as the mobile phase and the column packed with ODS (150 mm x 4.6 mm, 5 microns) as a fixed phase and the flow rate was 1.0 ml/min. FA was detected at 320 nm wave length. The internal standard was coumarin. The serum samples were treated by the water-boiling method. Peak of FA in serum was recognized by photodiode-array detection.


RESULTS
FA and internal standard were separated completely under the condition described as above. FA was linear in the range of (40.16-8032 ng/ml) (r = 0.9975). The CV was less than 10%, the average recovery was 100.3%, and the limit concentration in serum was 25.1 ng/ml. PK was in accordance with open bicameral model.


CONCLUSIONS
This method can be applied to clinical pharmacokinetic study of FA.
Sun
S
S
Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Huang
X
X
Zhang
L
L
Ren
P
P
Chang
Z
Z
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003373:Coumaric Acids / Q000097:blood / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005260:Female; D006801:Humans; D030004:Ligusticum; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C004999:ferulic acid; C101574:chuangxiong extract