Identification of Salmonella enteritidis outer membrane proteins expressed during attachment to human intestinal epithelial cells.
OBJECTIVE
To express and identify the Salmonella enterica ser. Enteritidis outer membrane proteins that are involved in the attachment to human intestinal epithelial cells.


RESULTS
Salmonella Enteritidis attachment proteins were expressed by infection of human intestinal epithelial cells (int-407) with Salm. Enteritidis strain SE28, followed by pulse labelling with [35S]-methionine. Outer membrane proteins were separated by TritonX-114 phase partitioning, and were detected by Salm. Enteritidis-specific polyclonal antibody. Outer membrane proteins of molecular weights 82.3, 75.6, 49.3, 35.5 and 19.3 kDa were newly synthesized. Expression of 31.2 and 16 kDa proteins was up-regulated, whereas the expression of 40.7 kDa protein was down-regulated. Polyclonal antibodies against the 82.3 and 75.6 kDa proteins significantly (P < 0.05) reduced the binding of Salm. Enteritidis to int-407 cells in vitro.


CONCLUSIONS
Outer membrane proteins 82.3 and 75.6 kDa are potentially involved in the attachment of Salm. Enteritidis to the intestinal mucosa.


CONCLUSIONS
Outer membrane proteins 82.3 and 75.6 kDa identified in this study could be used as potential vaccines to block or reduce Salm. Enteritidis colonization in chickens.
Fadl
A A
AA
Department of Pathobiology, University of Connecticut, Storrs, CT 06269, USA.
Venkitanarayanan
K S
KS
Khan
M I
MI
D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D012480:Salmonella Infections / Q000382:microbiology; D012477:Salmonella enteritidis / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology
D001425:Bacterial Outer Membrane Proteins