Specific attachment of aqueous-based microcapsules to macrophages, B cells, and dendritic cells in vitro.
Microcapsules were previously prepared composed of aqueous anionic polymers (e.g. alginate) and aqueous amines (e.g. spermine) and it was found that the aqueous-based microcapsules enhanced rotavirus-specific immune responses after oral or parenteral immunization of mice. In these studies, one has modified the amine moiety of aqueous-based microcapsules to bind covalently to avidin and the avidin-bearing microcapsules were linked to biotinylated antibodies specific for surface markers on murine macrophages, dendritic cells, or B cells. Using fluorescence flow cytometry, it was found that antibody-coated microcapsules bound specifically to antigen-presenting cells (APC) in vitro. The availability of APC-specific microcapsules should allow for the uptake of antigens by specific APC, and further one's understanding of the relative capacities of different APC to induce antigen-specific immune responses.
Brubaker
J O
JO
Division of Immunologic and Infectious Diseases, The Children's Hospital of Philadelphia, and The University of Pennsylvania School of Medicine, 19104, USA.
Macartney
K K
KK
Speaker
T J
TJ
Offit
P A
PA
D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D001360:Avidin / Q000737:chemistry; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D001710:Biotin / Q000737:chemistry; D002214:Capsules / Q000493:pharmacokinetics*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D007107:Immune System / Q000166:cytology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D014867:Water / Q000737:chemistry
D000906:Antibodies; D000954:Antigens, Surface; D002214:Capsules; D014867:Water; D001360:Avidin; D001710:Biotin