title: Characterization of a novel testicular form of human hormone-sensitive lipase.
abstract: Hormone-sensitive lipase (HSL) is an esterase and lipase, which are essential for spermatogenesis. Two HSL mRNAs are expressed in human testis. A long form is encoded by a testis-specific exon and nine exons common to testis and adipocyte HSL. Here we show that the short-form 3.3-kb mRNA possesses a unique 5' end that is transcribed from a novel testis-specific exon. The corresponding protein is similar to the 775-amino-acid-long adipocyte HSL. Immunohistochemistry experiments on human testis sections revealed that the long form is strictly expressed in haploid germ cells whereas the short form is expressed in interstitial and tubular somatic cells as well as premeiotic germ cells.
authors.affiliation: INSERM Unit 317, Institut Louis Bugnard, Université Paul Sabatier, Hôpital Rangueil, Toulouse, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Mairal
authors.forename: Nathalie
authors.initials: N
authors.lastname: Melaine
authors.forename: Henrik
authors.initials: H
authors.lastname: Laurell
authors.forename: Jacques
authors.initials: J
authors.lastname: Grober
authors.forename: Lena Stenson
authors.initials: LS
authors.lastname: Holst
authors.forename: Thierry
authors.initials: T
authors.lastname: Guillaudeux
authors.forename: Cecilia
authors.initials: C
authors.lastname: Holm
authors.forename: Bernard
authors.initials: B
authors.lastname: Jégou
authors.forename: Dominique
authors.initials: D
authors.lastname: Langin
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008954:Models, Biological; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013091:Spermatogenesis; D002787:Sterol Esterase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D013737:Testis / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D002787:Sterol Esterase