[Research on genetic variation of heart fatty acid-binding protein gene in ten pig breeds].
The genetic variation of heart fatty acid-binding protein (H-FABP) gene in 561 pigs including Duroc, Landrace, Large Yorkshire, Nanchang white pig, Erhualian, Meishan, Yushan black pig, Leping spotted pig, Jinhua black head-hind pig and Shanggao black head-hind pig were detected by PCR-RFLP with Hinf I, Hae III and Msp I. The results showed as follows: (1) Nanchang white pig presented only HH genotype while other breeds varied at the Hinf I-RFLP site; (2) The exotic breeds including Duroc, Landrace, Large Yorkshire and the native breed Nanchang white pig were proved to be polymorphic while the five Chinese local breeds presented no variation at the Hae III-RFLP site; (3) Among the tested breeds only Duroc presented variation at the Msp I-RFLP site. It is noted that all the Chinese local breeds present as AADD--genotypes.
Lin
Wan-Hua
WH
Jiangxi Provincial Key Laboratory for Animal Biotechnology, Jiangxi Agricultural University, Nanchang 330045, China.
Huang
Lu-Sheng
LS
Ren
Jun
J
Deng
Su-Hua
SH
Wang
Wen-Jun
WJ
Liu
Bao-Sheng
BS
Zhou
Li-Hua
LH
Chen
Cong-Ying
CY
D000818:Animals; D021461:Animals, Outbred Strains / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins; D014644:Genetic Variation; D005838:Genotype; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D013552:Swine / Q000235:genetics*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins