title: Application of random amplified polymorphic DNA (RAPD) analysis for identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets.
abstract: A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
authors.affiliation: Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Asensio
authors.forename: Isabel
authors.initials: I
authors.lastname: González
authors.forename: Alicia
authors.initials: A
authors.lastname: Fernández
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: Esther
authors.initials: E
authors.lastname: Lobo
authors.forename: Pablo E
authors.initials: PE
authors.lastname: Hernández
authors.forename: Teresa
authors.initials: T
authors.lastname: García
authors.forename: Rosario
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D017931:DNA Primers; D005399:Fishes / Q000235:genetics*; D005515:Food Labeling; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017747:Seafood / Q000592:standards*; D013045:Species Specificity
chemical_list: D017931:DNA Primers