title: Cellular localization of orexin receptors in human adrenal gland, adrenocortical adenomas and pheochromocytomas.
abstract: Orexin-A and -B are hypothalamic peptides derived from a precursor called prepro-orexin and related with the regulation of the energy balance and arousal. They act on G protein receptors named orexin receptor 1 (OX1R) and orexin receptor 2 (OX2R). In the present study, we used immunohistochemical techniques to detect the distribution of OXR in normal human adrenal gland and adrenal tumours (adrenocortical adenomas and pheochromocytomas). OX1R was expressed in the cortex of the normal human adrenal gland (glomerulosa, fasciculata and reticular zones) and OX2R was located in the medulla (epinephrine and norepinephrine cells). By the double immunofluorescence techniques, we demonstrated that virtually all medullar cells (epinephrine and norepinephrine cells) expressed OX2R. As was expected, according to the results obtained in normal tissues, cortical tumours (adrenocortical adenomas) were positive for OX1R but not for OX2R and conversely, medullar tumours (pheochromocytomas) expressed only OX2R.
authors.affiliation: Department of Morphological Sciences, Faculty of Medicine, Clinical University Hospital, University of Santiago de Compostela, c/ San Francisco s/n, E-15782, Santiago de Compostela, Spain.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Blanco
authors.forename: Tomás
authors.initials: T
authors.lastname: García-Caballero
authors.forename: Máximo
authors.initials: M
authors.lastname: Fraga
authors.forename: Rosalía
authors.initials: R
authors.lastname: Gallego
authors.forename: Juan
authors.initials: J
authors.lastname: Cuevas
authors.forename: Jerónimo
authors.initials: J
authors.lastname: Forteza
authors.forename: Andrés
authors.initials: A
authors.lastname: Beiras
authors.forename: Carlos
authors.initials: C
authors.lastname: Diéguez
mesh_terms: D000293:Adolescent; D000302:Adrenal Cortex / Q000378:metabolism; D000311:Adrenal Glands / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism; D018246:Adrenocortical Adenoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D064446:Orexin Receptors; D010673:Pheochromocytoma / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide / Q000096:biosynthesis / Q000378:metabolism*; D014018:Tissue Distribution / Q000502:physiology
chemical_list: C578626:HCRTR2 protein, human; D064446:Orexin Receptors; D043562:Receptors, G-Protein-Coupled; D018013:Receptors, Neuropeptide