title: In vitro effect of bioactive compounds on influenza virus specific B- and T-cell responses.
abstract: In vitro studies have demonstrated positive effects of bioactive compounds on several functions of the immune system. In the present study, 25 of such compounds were tested for their immune modulating properties on influenza virus specific human B- and T-cell responses in vitro. One of these compounds, N-acetyl-L-cysteine was shown to increase influenza virus specific lymphocyte proliferation and interferon(IFN)-gamma production at a concentration of 1.0 mmol/l. Furthermore, N-acetyl-L-cysteine was found to enhance a specific activity of two influenza specific CD8+ cytotoxic T-lymphocyte clones directed towards HLA-A*0201 and HLA-B*2705 restricted epitopes. A second compound, chlorogenic acid, was shown to enhance antigen specific proliferation of lymphocytes in three out of four donors, at concentrations of 10-50 micromol/l. Neither of the two compounds exhibited a positive effect on the production of influenza virus specific antibodies by human peripheral blood mononuclear cells in vitro.
authors.affiliation: Erasmus University Rotterdam, Institute of Virology, Dr Molewaterplein 50, 3015 GE, Rotterdam, The Netherlands.
authors.forename: A C M
authors.initials: AC
authors.lastname: Boon
authors.forename: A P
authors.initials: AP
authors.lastname: Vos
authors.forename: Y M F
authors.initials: YM
authors.lastname: Graus
authors.forename: G F
authors.initials: GF
authors.lastname: Rimmelzwaan
authors.forename: A D M E
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D002726:Chlorogenic Acid / Q000494:pharmacology; D019587:Dietary Supplements / Q000032:analysis; D004353:Drug Evaluation, Preclinical; D005504:Food Analysis; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D018977:Micronutrients / Q000494:pharmacology*; D009975:Orthomyxoviridae / Q000276:immunology*; D010936:Plant Extracts / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D018977:Micronutrients; D010936:Plant Extracts; D002726:Chlorogenic Acid; D007371:Interferon-gamma; D000111:Acetylcysteine