title: Dermal nevus cells from congenital nevi cannot penetrate the dermis in skin reconstructs.
abstract: Congenital nevi are composed of pigment cells bearing common features with melanocytes but showing altered differentiation which leads to nesting and dermal involvement. Using a dead de-epidermized dermis seeded with a combination of keratinocytes and various sources of pigment cells (normal melanocytes, dermal nevus cells from congenital nevi, Bowes melanoma cells), we have studied the formation of nests and the dermal migration of pigment cells together with their secretion profiles of matrix metalloproteinases (MMP). Dermal fibroblasts were also used as control cells in epidermal reconstructs. Besides their morphologic features, the absence of pigment donation to keratinocytes was the major characteristic of dermal nevus cells. A positive correlation was established between the increasing percentage of seeded nevus cells and the patchy pigmentation of reconstructs, as well as the clustering of cells in junctional nests. However, the presence of nevus cells in the dermis of reconstructs was never detected, whereas melanoma cells and dermal fibroblasts could invade the dermis during the time span of the experiments. MMP9 was never expressed in congenital dermal nevus cells but pro-MMP2 was constitutively expressed by all strains of congenital nevus cells and dermal fibroblasts. Melanocytes produced comparable amounts of both pro-MMP2 and pro-MMP9, and Bowes melanoma cells secreted a marginal level of pro-MMP2. In view of their three-dimensional behaviour and secretion of MMPs, we propose that dermal congenital nevus cells correspond to an intermediate status of differentiation between normal melanocytes and melanoma cells. Activation of MMPs by a cofactor or the activation of another signalling pathway seems necessary to induce the dermal passage of nevus cells.
authors.affiliation: Laboratoire PMTG, Unité de Dermatologie, Université Victor Ségalen, Bordeaux, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Gontier
authors.forename: Muriel
authors.initials: M
authors.lastname: Cario-André
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lepreux
authors.forename: Pierre
authors.initials: P
authors.lastname: Vergnes
authors.forename: Jozef
authors.initials: J
authors.lastname: Bizik
authors.forename: Jean-Etienne
authors.initials: JE
authors.lastname: Surlève-Bazeille
authors.forename: Alain
authors.initials: A
authors.lastname: Taïeb
mesh_terms: D000293:Adolescent; D002452:Cell Count; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D020405:Dermis / Q000503:physiopathology*; D004792:Enzyme Precursors / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009506:Nevus / Q000151:congenital / Q000473:pathology*; D010860:Pigments, Biological; D012867:Skin / Q000166:cytology; D012878:Skin Neoplasms / Q000151:congenital / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D004792:Enzyme Precursors; D010860:Pigments, Biological; D018093:Gelatinases; D008666:Metalloendopeptidases; C069860:progelatinase