[The effect of medium viscosity on actin polymerization].
A comparative study of the influence of two factors--excluded volume effect, and medium viscosity--on actin polymerization was carried out. Dextrane--500 (1%), poly(ethylene)glycol--6000 (2.7%), Ficol (1.8%), methyl cellulose (0.04%), saccharose (10%), and glycerine (10%) were used for creating a viscous medium. The concentrations of these agents in actin solution made approximately the same viscosity commensurable with the cytoplasm viscosity. By this it was possible to bring conditions of actin polymerization in vitro closer to those in vivo. It is shown that the medium viscosity in contrast to the excluded volume effect, prolongs the nucleation phase during actin polymerization. A conclusion is made that in the cell both factors can be involved in the regulation of actin filament formation.
Pershina
V P
VP
Institute of Cytology RAS, St. Petersburg.
Petrov
Iu P
IuP
Pinaev
G P
GP
D000199:Actins / Q000737:chemistry*; D000818:Animals; D019281:Dimerization; D011108:Polymers; D011817:Rabbits; D014783:Viscosity
D000199:Actins; D011108:Polymers