Polymerase chain reaction-restriction fragment length polymorphism of ribosomal internal transcribed spacer region analysis on polyacrylamide gel electrophoresis reveals two haplotypes coexisting in Myzus persicae.
Aphids are geographically and economically the most important group of phytophagous insects. They are responsible for various damage on cultivated plants. In our laboratory, we are interested in their ability to transmit phytoviruses such as Rhabdovirus, Cucumovirus, Potyvirus, and Caulimavirus which cause many crop diseases. The present work deals with the study of vectors as analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of internal transcribed spacer (ITS) regions. Electrophoresis of PCR-RFLP products using EcoRV, AluI and DraI restriction enzymes on 10% polyacrylamid gel reveals the coexistence of two different haplotypes in the same individual genome. This result seems to explain some features of aphid evolution.
Faten
Raboudi
R
Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Tunis, Tunisie. faten_rf@prontomail.com
Mohamed
Marrakchi
M
Mohamed
Makni
M
D000818:Animals; D001042:Aphids / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D017344:Genes, Insect; D006239:Haplotypes; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
D021903:DNA, Ribosomal Spacer