Detection of ethylene receptor protein Cm-ERS1 during fruit development in melon (Cucumis melo L.).
Antibodies against melon ethylene receptor, Cm- ERS1 was prepared. Cm-ERS1 protein formed a disulphide-linked homodimer and it was present in microsomal membranes but not in soluble fractions. Cm-ERS1 protein was present at high levels in melon fruit during early developmental stages. This transition pattern was also observed in another melon cultivar.
Takahashi
Hidenori
H
Plant Biotechnology Institute, Ibaraki Agricultural Centre, Ago 3165-1, Iwama, Nishi-ibaraki, Ibaraki, 319-0292 Japan.
Kobayashi
Toshihiro
T
Sato-Nara
Kumi
K
Tomita
Ken-o
KO
Ezura
Hiroshi
H
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D028462:Cucumis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; C036216:ethylene