Engineered BGMK cells for sensitive and rapid detection of enteroviruses.
Decay-accelerating factor (DAF) has been reported to be a cellular receptor for several enteroviruses. Buffalo green monkey kidney (BGMK) cells expressing human DAF (BGMK-hDAF cells) showed increased susceptibility and sensitivity to several types of enteroviruses compared to wild-type BGMK cells. When 17 frozen positive clinical samples were tested, BGMK cells detected 8 and BGMK-hDAF cells detected 16. Since the CaCo-2 cell line has been documented to support the replication of most enteroviruses, CaCo-2 cells were mixed with BGMK-hDAF cells in order to increase the number of viruses detected. Thirty-four frozen clinical samples that previously had tested positive for enteroviruses were tested, and the following numbers were detected: 33 of 34 by CaCo-2/BGMK-hDAF cells, 29 of 34 by CaCo-2/BGMK cells, 28 of 34 by H292/RD (E-mix A) and A-549/BGMK (E-mix B) cells, and 26 of 34 by MRC-5 and pRhMK cells.
Huang
Yung T
YT
Department of Pathology, University Hospital of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106, USA. yth@po.cwru.edu
Yam
Paul
P
Yan
Huimin
H
Sun
Yan
Y
D000818:Animals; D018958:CD55 Antigens / Q000235:genetics / Q000378:metabolism*; D018938:Caco-2 Cells; D002460:Cell Line; D029822:Enterovirus B, Human / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology*; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D007668:Kidney; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013997:Time Factors; D014162:Transfection
D018958:CD55 Antigens; D011991:Receptors, Virus