title: Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk.
abstract: A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of metformin in human plasma and breast milk is described. After proteins were precipitated with acetonitrile, metformin and the internal standard buformin were resolved on a cation-exchange column and detected by UV detection at 236 nm. Standard curves were linear over the concentration range 20.0-4000 microg/l. Intra- and inter-day coefficients of variation were <9.0% and the limit of quantification was around 20 microg/l.
authors.affiliation: Department of Clinical Pharmacology, Christchurch Hospital, New Zealand. mei.zhang@cdhb.govt.nz
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Grant A
authors.initials: GA
authors.lastname: Moore
authors.forename: Michael
authors.initials: M
authors.lastname: Lever
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Gardiner
authors.forename: Carl M J
authors.initials: CM
authors.lastname: Kirkpatrick
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Begg
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000097:blood; D008687:Metformin / Q000032:analysis* / Q000097:blood; D008895:Milk, Human / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007004:Hypoglycemic Agents; D008687:Metformin