Chromatographic characterization of synthetic peptides: SPf66 malaria vaccine.
The development and validation of a quantitative size-exclusion chromatography (SEC) method for SPf66 malaria vaccine was achieved. The results show the reliability of the analytical method for the intended use. SPf66 malaria vaccine characterization was perforrmed using both relative techniques such as the conventional SEC and absolute techniques: mass spectrometry and multi-angle laser-light scattering detection. The relative and absolute molecular masses were in the 4600-18,000 Da range. The results clearly indicate the presence of the monomer and dimer species, whereas the third species could be the trimer or tetramer.
Santoveña
Ana
A
Departamento de Ingeniería Química y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de La Laguna, Tenerife, Spain.
Oliva
Alexis
A
Guzman
Fanny
F
Patarroyo
Manuel E
ME
Llabrés
Matías
M
Fariña
José B
JB
D002850:Chromatography, Gel / Q000379:methods*; D017780:Malaria Vaccines / Q000737:chemistry*; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry*; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C066269:SPf66 protein, Plasmodium