Avian anticoccidial activity of a novel membrane-interactive peptide selected from phage display libraries.
In the present work, we describe the discovery of PW2, a novel peptide presenting in vitro activity against Eimeria acervulina and E. tenella sporozoites. PW2 was selected from phage display (Ph.D.) peptide libraries by an alternative method of panning using living purified E. acervulina sporozoites as targets. Our results showed that the peptide disrupts the sporozoite pellicle, resembling the effect caused by most natural antimicrobial peptides. PW2 peptide was also effective against fungi and showed low activity against Toxoplasma gondii tachyzoites, but no activity against Trypanosoma cruzi, Crithidia fasciculata epimastigotes, and bacteria. Additionally, the parasiticidal concentrations of PW2 produced a very low lytic effect on mammalian and avian cells. The effectiveness against Eimeria sporozoites and the absence of adverse effects to host cells indicates that PW2 may be used as a model to generate new drugs for the control of avian coccidiosis.
da Silva
Arnaldo
A
Centro de Biologia Molecular e Engenharia Genetica, Universidade Estadual de Campinas, Cidade Universitaria Zeferino Vaz, 13083-970, SP, Campinas, Brazil.
Kawazoe
Urara
U
Freitas
Fabio F T
FF
Gatti
Maria Silvia V
MS
Dolder
Heidi
H
Schumacher
Robert Ivan
RI
Juliano
Maria Aparecida
MA
da Silva
Marcio Jose
MJ
Leite
Adilson
A
D000595:Amino Acid Sequence; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D002645:Chickens / Q000469:parasitology*; D003049:Coccidiostats / Q000494:pharmacology*; D004539:Eimeria / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D004912:Erythrocytes; D006461:Hemolysis; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000166:cytology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D021261:Parasitic Sensitivity Tests; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D011817:Rabbits
D003049:Coccidiostats; D036341:Intercellular Signaling Peptides and Proteins; C455319:PW2 peptide; D019151:Peptide Library; D010455:Peptides