title: Cloning, characterization, and expression of calcyphosine 2, a novel human gene encoding an EF-hand Ca(2+)-binding protein.
abstract: Calcyphosine is a calcium-binding protein containing four EF-hand domains, initially identified as thyroid protein p24. It was first cloned and its counterparts in rabbit, human, and mouse, crayfish and lobster of invertebrate were also cloned. Here we describe the cloning and characterization of a novel human calcyphosine gene. The 3829-bp cDNA encodes a EF-hand Ca(2+)-binding protein homologous to the dog calcyphosine. It also contains two EF-hand Ca(2+)-binding motif. It is abundantly expressed in many tissues including by RT-PCR analysis and believed to play important role in calcium signaling. It was mapped to human genome 12q15.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Jin-zhong
authors.initials: JZ
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qingshan
authors.initials: Q
authors.lastname: Huang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Yumin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004285:Dogs; D020832:EF Hand Motifs; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: C059605:CAPS protein, human; C449654:CAPS2 protein, human; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger