title: Nitric oxide regulates matrix metalloprotease-13 expression and activity in endothelium.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) are synthesized in response to diverse stimuli including cytokines, growth factors, hormones, and oxidative stress.
  
  
  METHODS
  Bovine aortic endothelial cells (BAEC) were stimulated with nitric oxide (NO) and MMP-13 expression and activity was assayed.
  
  
  RESULTS
  NO transcriptionally regulated matrix metalloproteinase-13 (MMP-13) expression in BAEC, while the cGMP analog 8Br-cGMP mimicked the effect of NO. In addition NO also stimulated the proteolytic processing of MMP-13 from the pro-enzyme to the final active form in a dose dependent manner.
  
  
  CONCLUSIONS
  NO transcriptionally regulates MMP expression and activity in the vascular endothelium. This effect of NO may be of pathophysiological importance in the context of angiogenesis, inflammation or atherogenesis.
authors.affiliation: Centro de Investigaciones Biológicas, Instituto "Reina Sofía" de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006 Madrid, Spain. carlosz@cib.csic.es
authors.forename: Carlos
authors.initials: C
authors.lastname: Zaragoza
authors.forename: Milagros
authors.initials: M
authors.lastname: Balbín
authors.forename: Carlos
authors.initials: C
authors.lastname: López-Otín
authors.forename: Santiago
authors.initials: S
authors.lastname: Lamas
mesh_terms: D000818:Animals; D002417:Cattle; D017364:Collagenases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D006834:Hydrazines / Q000008:administration & dosage / Q000494:pharmacology; D053509:Matrix Metalloproteinase 13; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000008:administration & dosage / Q000494:pharmacology; D009589:Nitrogen Oxides
chemical_list: D006834:Hydrazines; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D017364:Collagenases; D053509:Matrix Metalloproteinase 13