title: Development of antigen induced colitis in SCID mice reconstituted with spleen derived memory type CD4(+) CD45RB(+) T cells.
abstract: OBJECTIVE
  Enteric bacterial and/or food antigens may be crucial in the development of colitis but little is known of the exact mechanism of antigen specific reactions in this condition. The aim of this study was to determine whether systemically primed antigen specific CD4(+) T cells containing both CD45RB(high) and CD45RB(low) populations participate as a pathogenic subset that in turn leads to inflammatory reactions selectively in the large intestine.
  
  
  METHODS
  SCID mice were reconstituted with splenic CD4(+) CD45RB(+) T cells or CD4(+) CD45RB(low) T cells isolated from donor mice systemically primed with ovalbumin (OVA) plus CFA. The reconstituted mice were then fed OVA for several weeks.
  
  
  RESULTS
  Reconstitution of SCID mice with OVA primed splenic CD4(+) T cells, containing populations of CD45RB(high) and CD45RB(low), resulted in the development of colitis by 4-5 weeks following repeated administration of oral OVA. Histopathological study revealed thickened wall, inflammatory cell infiltration, crypt elongation, and loss of goblet cells in the large intestine. The CD4(+) CD45RB(low) population of cells extracted from the affected large intestine secreted high levels of interferon gamma (IFN-gamma) and tumour necrosis factor alpha (TNF-alpha) at the protein and mRNA levels. Administration of neutralising antibodies to TNF-alpha, but not to IFN-gamma, prevented the development of colitis. Furthermore, adoptive transfer with OVA primed splenic CD4(+) CD45RB(low) T cells evoked severe colitis.
  
  
  CONCLUSIONS
  These results demonstrate that systemically primed activated/memory CD4(+) CD45RB(low) T cells can mediate the development of specific antigen induced colitis in SCID mice, and also that TNF-alpha is critical in the induction of this type of colitis. Our results contrast with those from studies in some colitis models in which CD45RB(low) T cells appeared to prevent colitis through secretion of immunosuppressive cytokines.
authors.affiliation: Department of Mucosal Immunology, Research Institute for Microbial Diseases, Osaka University, Osaka 565, Japan.
authors.forename: M-N
authors.initials: MN
authors.lastname: Kweon
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: M H
authors.initials: MH
authors.lastname: Jang
authors.forename: N
authors.initials: N
authors.lastname: Suenobu
authors.forename: H
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003092:Colitis / Q000276:immunology* / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D018450:Disease Progression; D007420:Intestine, Large / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000032:analysis; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D010047:Ovalbumin / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015431:Weight Loss
chemical_list: D000941:Antigens; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D010047:Ovalbumin; D017493:Leukocyte Common Antigens