Interference of MI-D, a new mesoionic compound, on artificial and native membranes.
MI-D (4-phenyl-5-(4-nitrocinnamoyl)-1,3,4-thiadiazolium-2-phenylamine chloride), a new mesoionic compound, decreased the rate of swelling induced by valinomycin-K+, as well as induced swelling in the presence of nigericin-K+. Shrinkage was also affected, suggesting interference with the inner mitochondrial membrane, which would affect both fluidity and elasticity. Fluorescence polarization of DPH and DPH-PA, probing the core and outer regions respectively, of the DMPC and native membranes, indicated that MI-D shifts the midpoint of phase transition to higher values and orders of the fluid phase. These alterations in membrane fluidity are thus related to MI-D effects on the energy-linked functions of mitochondria.
Cadena
Silvia M S C
SM
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, Brasil. silvia.cadena@utp.br
Carnieri
Eva G S
EG
Echevarria
Aurea
A
de Oliveira
Maria Benigna Martinelli
MB
D000818:Animals; D002934:Cinnamates / Q000494:pharmacology*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives / Q000378:metabolism; D005454:Fluorescence Polarization; D007425:Intracellular Membranes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D007476:Ionophores / Q000037:antagonists & inhibitors; D007700:Kinetics; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008567:Membranes, Artificial; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D009550:Nigericin / Q000037:antagonists & inhibitors; D009994:Osmolar Concentration; D010539:Permeability / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013830:Thiadiazoles; D013844:Thiazoles / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000494:pharmacology*; D014634:Valinomycin / Q000037:antagonists & inhibitors
C116348:4-phenyl-5-(4-nitrocinnamoyl)-1,3,4-thiadiazolium-2-phenylamine; D002934:Cinnamates; D007476:Ionophores; D008567:Membranes, Artificial; D013830:Thiadiazoles; D013844:Thiazoles; D014475:Uncoupling Agents; D004161:Diphenylhexatriene; D014634:Valinomycin; D009550:Nigericin; D011188:Potassium