Protective effects of Polygalae root in experimental TNBS-induced colitis in mice.
In this study, the protective effect of Polygalae root was evaluated in a murine model of experimental colitis by intrarectal injection with 2,4,6-trinitrobenzene sulfonic acid (TNBS). Polygalae root, given orally at 2, 4 g/kg body weight of herbs once a day consecutively for 9 days, could recover the lost body weight and decrease the gross rectal bleeding. Polygalae root also reduced the degree of inflammation and improved significantly the histological changes such as infiltration by polymorphonuclear leukocytes and multiple erosive lesions. Furthermore, the cytokine production of intraepithelial lymphocytes was analyzed. The results showed that IFN-gamma was increased, but IL-4 was decreased in TNBS-induced colitis, when those were compared with the sham controls. But the administration of Polygalae root to TNBS-induced colitis mice showed lower production of IFN-gamma and higher production of IL-4 than the TNBS-induced colitis. These results suggest that the protective effects of Polygalae root against the TNBS colitis may be associated with the regulation of cytokine production of intraepithelial lymphocytes.
Hong
Tie
T
Department of Bioregulatory Function, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyou-Ku, Tokyo 113-8655, Japan. tie-tky@umin.ac.jp
Jin
Guang Bi
GB
Yoshino
Gen
G
Miura
Myota
M
Maeda
Yoko
Y
Cho
Shigefumi
S
Cyong
Jong Chol
JC
D000818:Animals; D003092:Colitis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005260:Female; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy / Q000379:methods / Q000706:statistics & numerical data; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D029608:Polygalaceae; D013154:Spleen / Q000187:drug effects; D013950:Thymus Gland / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid
D016207:Cytokines; D010936:Plant Extracts; D014302:Trinitrobenzenesulfonic Acid