title: Biflavones of Ginkgo biloba stimulate lipolysis in 3T3-L1 adipocytes.
abstract: Ginkgo biloba L. biflavones were shown to increase cAMP phosphodiesterase activity and to stimulate skin microcirculation. The aim of this study was to investigate whether biflavones were able to stimulate lipolysis in adipocytes. Lipolysis was assayed in fully differentiated 3T3-L1 fat cells in the presence of biflavones at 0.005 - 100 microM. Cell viability was evaluated at 0.5 -100 microM. Theophylline and caffeine were used as reference compounds. Lipolytic activity in untreated cells was 0.62 +/- 0.15 micromoles glycerol/mg DNA/h. All biflavones except sciadopitysin stimulated lipolysis in a concentration-dependent fashion. Maximal stimulation was observed at 0.1 - 0.5 microM. At higher concentrations the effect diminished progressively and was lost at 100 microM. Only a partial loss of cell viability was observed with biflavones at 10 - 100 microM.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmacy, University of Milan, Milan, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Dell'Agli
authors.forename: Enrica
authors.initials: E
authors.lastname: Bosisio
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D044946:Biflavonoids; D002110:Caffeine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020441:Ginkgo biloba; D008066:Lipolysis / Q000187:drug effects*; D008516:Medicine, Chinese Traditional; D051379:Mice; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology
chemical_list: D044946:Biflavonoids; D005419:Flavonoids; D010936:Plant Extracts; C402623:bilobetin; C452984:isoginkgetin; C452983:sequoiaflavone; D002110:Caffeine; C077458:ginkgetin; C086243:sciadopitysin; C011164:amentoflavone; D013806:Theophylline