Antioxidant effects of 1,5-anhydro-D-fructose, a new natural sugar, in vitro.
The antioxidant effects of 1,5-anhydro-D-fructose (1,5-AF), a unique anhydrohexulose, were studied in 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution, in human cells along with lipid peroxidation of low-density lipoprotein (LDL). We have confirmed that 1,5-AF scavenges DPPH radicals directly in solution and inhibits the formation of hydrogen peroxide and superoxide anion, typical reactive oxygen species (ROS), induced by phorbol myristate acetate (PMA) in a dose-dependent manner in THP-1 cells. We also observed the dose-dependent antioxidant effects of 1,5-AF on copper-mediated LDL oxidation. These findings suggest that 1,5-AF might play a role in reducing the risk of atherosclerosis and may help prevent coronary heart disease.
Yamaji
Kazuyo
K
Department of Laboratory and Molecular Medicine, Faculty of Medicine, Kagoshima University, Kagoshima City, Japan. yamaji@m3.kufm.kagoshima-u.ac.jp
Sarker
Krishna Pada
KP
Maruyama
Ikuro
I
Hizukuri
Susumu
S
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015764:Bepridil / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D001685:Biological Factors; D001713:Biphenyl Compounds; D002241:Carbohydrates / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000008:administration & dosage / Q000378:metabolism; D005632:Fructose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages; D015394:Molecular Structure; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010851:Picrates; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage
D000975:Antioxidants; D001685:Biological Factors; D001713:Biphenyl Compounds; D002241:Carbohydrates; D016166:Free Radical Scavengers; D005609:Free Radicals; D008077:Lipoproteins, LDL; D010851:Picrates; D017382:Reactive Oxygen Species; D013481:Superoxides; D005632:Fructose; D017665:Hydroxyl Radical; C048927:1,5-anhydrofructose; D015764:Bepridil; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D013755:Tetradecanoylphorbol Acetate