Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts.
An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di- O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity- guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
Baez
Daniel Arrieta
DA
Instituto de Química, UNAM, Ciudad Universitaria, Coyoacán, CP 04510, Mexico DF, Mexico.
Vallejo
Gerardo Zepeda
GZ
Sánchez
Patricia Cano
PC
Valle
Matilde Breña
MB
Chilpa
Ricardo Reyes
RR
Estrada
Manuel Jiménez
MJ
D005591:Chemical Fractionation; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D008745:Methylation; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D029846:Senna Plant; D012996:Solutions
D009153:Mutagens; D010936:Plant Extracts; D012996:Solutions; D012431:Rutin; D011794:Quercetin