Genetic and immunochemical characterization of thiocyanate-degrading bacteria in lake water.
Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from activated sludge.
Yamasaki
Manabu
M
Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Matsushita
Yasuhiko
Y
Namura
Motonobu
M
Nyunoya
Hiroshi
H
Katayama
Yoko
Y
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D015153:Blotting, Western; D003470:Culture Media; D004275:DNA, Ribosomal / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013461:Sulfur Oxides / Q000378:metabolism; D013861:Thiocyanates / Q000378:metabolism*
D003470:Culture Media; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013461:Sulfur Oxides; D013861:Thiocyanates; C010063:carbonyl sulfide; D006867:Hydrolases; C074369:thiocyanate hydrolase; C031760:thiocyanate