title: Structural analysis of the rDNA intergenic spacer of Tuber borchii.
abstract: The sequence and characterisation of the entire nuclear rDNA intergenic spacer (IGS) for the genus Tuber are presented. Sequence analyses showed that the organisation of the Tuber borchii rDNA IGS is typical of rDNA spacers, consisting of a central repetitive region and flanking unique sequences on either side. Direct repeats, symmetry elements, tandem repeats and possible areas of recombination were found. The putative ends of the 25S and 17S rDNA were identified. The presence of 5S rDNA in the IGS region was excluded.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Universit degli Studi di Urbino, Via A. Saffi 2, 61029 Urbino (PU), Italy.
authors.forename: Pasquapina
authors.initials: P
authors.lastname: Ciarmela
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Luigi
authors.initials: L
authors.lastname: Cucchiarini
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D004275:DNA, Ribosomal