[Immunodiagnosis of neurocysticercosis: comparative study of antigenic extracts from Cysticercus cellulosae and Taenia crassiceps].
Different antigenic extracts of Taenia solium and Taenia crassiceps were evaluated in connection with the detection of antibodies in patients with neurocysticercosis aimed at selecting immunorelevant antigens for the diagnosis of neurocysticercosis by means of the immunoenzymatic assay and immunoblotting. The vesicular fluid of T. crassiceps proved to be more sensitive (100%) and specific (86%). On using the immunoblotting technique it was also observed that this extract was the most sensitive and specific. Within the protein profile of the antigen the band of 18 kDa was mostly recognized by the serum and cerebrospinal fluid of patients with neurocysticercosis. The vesicular fluid of T. crassiceps represents an alternative in the optimization of the diagnosis of neurocysticercosis in the serum and cerebrospinal fluid and in the substitution of T. solium antigens due to its high sensitivity and specificity and to its easy obtention under controlled laboratory conditions.
Rossi
N
N
Instituto de Inmunología Clínica, Universidad de los Andes, Mérida, Venezuela.
Rivas
I
I
Hernández
M
M
Urdaneta
H
H
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D003552:Cysticercus / Q000276:immunology*; D006801:Humans; D007159:Immunologic Tests; D020019:Neurocysticercosis / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth