Biochemical and biological properties of Trimeresurus jerdonii venom and characterization of a platelet aggregation-inhibiting acidic phospholipase A2.
Several biochemical and biological activities such as phospholipase A2, arginine esterase, proteolytic, L-amino acid oxidase, 5'nucleotidase, acetylcholinesterase, thrombin-like, anticoagulant, and hemorrhagic activities were determined for whole desiccated venom of Trimeresurus jerdonii. An acidic phospholipase (named TJ-PLA2) was purified by anionic exchange chromatography, gel filtration, and reverse phase HPLC. TJ-PLA2 had a molecular weight of 16,000 and a pI of 4.8. TJ-PLA2 was non-lethal to mice up to an i.p. dose of 15 mg/kg body weight and lacked neurotoxicity and myotoxicity. It induced edema in the footpads of mice. The purified enzyme inhibited ADP- and collagen-induced human platelet aggregation in a manner which was both dose- and time-dependent.
Lu
Qui-Min
QM
Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan.
Jin
Yang
Y
Wei
Ji-Fu
JF
Wang
Wan-Yu
WY
Xiong
Yu-Liang
YL
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003435:Crotalid Venoms / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004798:Enzymes / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006614:Hindlimb / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010741:Phospholipases A / Q000302:isolation & purification* / Q000633:toxicity; D054467:Phospholipases A2; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000633:toxicity; D017841:Trimeresurus
D003435:Crotalid Venoms; D004798:Enzymes; D010975:Platelet Aggregation Inhibitors; C029748:Trimeresurus venoms; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D010741:Phospholipases A; D054467:Phospholipases A2