title: Effect of Helicobacter pylori infection on gastric cell proliferation and genomic instability in a paediatric population of southern Italy.
abstract: BACKGROUND
  The incidence of gastric cancer is high in areas with a high prevalence of Helicobacter pylori infection. Cell transformation and tumour progression occur over a long period of time and markers of genomic instability usually precede morphological changes.
  
  
  OBJECTIVE
  To evaluate the effect of Helicobacter pylori infection on cell proliferation, DNA status and oncogene expression in children.
  
  
  METHODS
  Morphometric and immunohistochemical techniques were used to analyse DNA content, p53 and c-myc oncogene expression and cell proliferation on gastric biopsies of 53 children (27 Helicobacter pylori-negative and 26 Helicobacter pylori-positive).
  
  
  RESULTS
  Gastric mucosa was normal in 11% of Helicobacter pylori-positive and in 33% of Helicobacter pylori-negative subjects. Most children had chronic non-atrophic gastritis regardless of Helicobacter pylori infection, and only a minority of children affected by Helicobacter pylori had mild atrophic gastritis. Cell proliferation was significantly higher in children with Helicobacter pylori-positive gastritis than in those with Helicobacter pylori-negative gastritis. No metaplasia, dysplasia, p53 overexpression or altered DNA content was found in any child. Interestingly, 46% of children with and 29% without Helicobacter pylori infection had c-myc overexpression closely related to the cell proliferation rate.
  
  
  CONCLUSIONS
  Helicobacter pylori infection in children may coexist with a normal gastric mucosa, and it is not associated with genomic instability markers in cases of chronic gastritis.
authors.affiliation: Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy. genardo@tin.it
authors.forename: G
authors.initials: G
authors.lastname: Nardone
authors.forename: S
authors.initials: S
authors.lastname: Staibano
authors.forename: A
authors.initials: A
authors.lastname: Rocco
authors.forename: E
authors.initials: E
authors.lastname: Mezza
authors.forename: T
authors.initials: T
authors.lastname: Balzano
authors.forename: G
authors.initials: G
authors.lastname: Salvatore
authors.forename: A
authors.initials: A
authors.lastname: Staiano
authors.forename: V
authors.initials: V
authors.lastname: Donofrio
authors.forename: B
authors.initials: B
authors.lastname: Grazioli
authors.forename: G
authors.initials: G
authors.lastname: De Rosa
authors.forename: G
authors.initials: G
authors.lastname: Budillon
mesh_terms: D000293:Adolescent; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000235:genetics* / Q000382:microbiology* / Q000473:pathology; D005757:Gastritis, Atrophic / Q000235:genetics; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics; D016481:Helicobacter Infections / Q000235:genetics / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D008297:Male