title: [Immunohistochemical investigations of congenital pseudarthrosis of the tibia].
abstract: OBJECTIVE
  To investigate the etiology and pathology of congenital pseudarthrosis.
  
  
  METHODS
  Sixty-three specimens were taken from 21 CPT patients. The antibodies, which were used for immunohistochemical studies, were produced against (1) NF-200 or S-100 protein; (2) type I or III collagen; (3) PCNA. The control specimens were taken from neurofibromatosis and traumatic pseudarthrosis. SABC staining was performed.
  
  
  RESULTS
  Low positive expression of NF-200 (3.2%) and S-100 (4.8%) was noted in the CPT lesion, whereas high expression of NF-200 (85.2%) and S-100 (88.9%) in the lesion of neurofibromatosis. The soft tissues in CPT had a higher ratio of type III to I collagens in comparison with traumatic pseudarthrosis. Positive expression of PCNA (95.2%) was significantly higher in the soft tissues than that (2.5%) in traumatic pseudarthrosis.
  
  
  CONCLUSIONS
  CPT is a disease of non-neuro origin. Its occurrence has no direct relation with neurofibromatosis. CPT has its characteristic pathology in the periosteum rather than in the bone. The pathological fracture or non-union of CPT is caused by fibromatosis, which has a strong activity of cellular proliferation and corrosion. CPT has its pathological property similar to a tumor.
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
authors.forename: W
authors.initials: W
authors.lastname: Lei
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Lu
authors.forename: G
authors.initials: G
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D016900:Neurofilament Proteins / Q000032:analysis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011542:Pseudarthrosis / Q000151:congenital / Q000378:metabolism / Q000473:pathology*; D009418:S100 Proteins / Q000032:analysis; D013978:Tibial Fractures / Q000151:congenital / Q000378:metabolism / Q000473:pathology*
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D016900:Neurofilament Proteins; D018809:Proliferating Cell Nuclear Antigen; D009418:S100 Proteins