title: Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays.
abstract: PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3' end.
authors.affiliation: Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark. hbjorn@cbs.dtu.dk
authors.forename: H B
authors.initials: HB
authors.lastname: Nielsen
authors.forename: S
authors.initials: S
authors.lastname: Knudsen
mesh_terms: D000818:Animals; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D030541:Databases, Genetic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data*; D016133:Polymerase Chain Reaction; D012984:Software
chemical_list: D017931:DNA Primers