Cashew-tree (Anacardium occidentale L.) exudate gum: a novel bioligand tool.
The potential of bioaffinity as a tool for the study of biological-recognition mechanisms is gaining increasing value. The search continues for alternative products that can be obtained from renewable sources, such as the bark exudate gum from the cashew tree (Anacardium occidentale L.), which grows wild in many tropical and subtropical countries. Its potential use as a chromatographic matrix and/or for bioaffinity ligand for proteins (lectins) has been investigated. The crude gum was cross-linked in order to obtain a kind of chromatographic matrix (gel). To evaluate the gum's ability to retain glycoproteins (lectins), affinity chromatography was performed and, in addition, the reological behaviour of the gum was characterized.
da Silveira Nogueira Lima
Raquel
R
Lectins and glycoconjugates Laboratory (LABLEC), Federal University of Ceará, Campus do Pici, Block 907, P.O. Box 6020, Fortaleza, Ceará 60455-900, Brazil. rsnlima@yahoo.com
Rabelo Lima
Jacira
J
Ribeiro De Salis
Celio
C
de Azevedo Moreira
Renato
R
D027926:Anacardiaceae / Q000737:chemistry*; D001709:Biotechnology; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D008024:Ligands; D008055:Lipids / Q000032:analysis; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D037121:Plant Lectins; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012212:Rheology; D012639:Seeds / Q000737:chemistry; D012995:Solubility
D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D008055:Lipids; D010936:Plant Extracts; D037121:Plant Lectins; D011134:Polysaccharides