Entrainment in solution of an oscillating NADH oxidase activity from the bovine milk fat globule membrane with a temperature-compensated period length suggestive of an ultradian time-keeping (clock) function.
Entrainment in solution of an oscillating activity with a temperature compensated period of 24 min is described for a NADH oxidase (NOX) activity of the bovine milk fat globule membrane, a derivative of the mammary epithelial cell plasma membrane. The period of 24 min remained unchanged at 17 degrees C, 27 degrees C and 37 degrees C whereas the amplitude approximately doubled with each 10 degree C rise in temperature (Q(10)congruent with 2). The periodicity was observed with both intact milk fat globule membranes and with detergent-solubilized membranes, demonstrating that the oscillations did not require an association with membranes. The periodicity was not the result of instrument variation or of chemical interactions among reactants in solution. Preparations with different periodicities entrained (autosynchronized) when mixed. Upon mixing, the preparations exhibited two oscillatory patterns but eventually a single pattern representing the mean of the farthest separated maxima of the two preparations analyzed separately emerged. The cell surface NOX protein is the first reported example of an entrainable biochemical entity with a temperature-compensated periodicity potentially capable of functioning as an ultradian or circadian clock driver.
Morré
D James
DJ
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette. IN 47907, USA. morre@pharmacy.purdue.edu
Lawler
Juliana
J
Wang
Sui
S
Keenan
Thomas W
TW
Morré
Dorothy M
DM
D000818:Animals; D001683:Biological Clocks; D002417:Cattle; D005583:Fourier Analysis; D008566:Membranes; D008892:Milk / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D000074624:NADPH Oxidase 1; D010084:Oxidation-Reduction; D010507:Periodicity; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D009097:Multienzyme Complexes; D012996:Solutions; D009243:NAD; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D000074624:NADPH Oxidase 1