Effects of 2-deoxy-D-glucose on DNA repair and mutagenesis in UV-irradiated yeast.
We have studied the effects of 2-deoxy-D-Glucose (2-DG) on the recovery of potentially lethal damage (PLDR), repair of chromosomal DNA, cyclobutane pyrimidine dimers (CPDs), reverse mutation and gene-conversion in UVC (254 nm) irradiated yeast. As studied by pulsed-field gel electrophoresis, post-irradiation chromosomal DNA repair kinetics in a phosphate buffer (PB) with 10 mM glucose (G) was biphasic, where the first phase exhibited a decrease and the second phase showed an increase in the band intensities. A post-irradiation treatment in PB + G (10 mM) with 2-DG (10, 20, 50 mM) reduced the decrease in the DNA band intensities in the first phase of DNA repair. As compared to a post-irradiation (125 J/m2) treatment in PB + G (10 mM), a treatment in PB + G (10 mM) + 2-DG (10 mM) showed a decreased PLDR, but increased revertants and gene-convertants.
Bala
M
M
Department of Molecular Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi-110054, India. balainma44@hotmail.com
Sharma
A K
AK
Goel
H C
HC
D002875:Chromosomes / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D016296:Mutagenesis / Q000187:drug effects*; D012441:Saccharomyces cerevisiae; D014466:Ultraviolet Rays
D003847:Deoxyglucose