Experimental study on the apoptosis of the astrocytes and the oligodendrocytes induced by Ca(2+) reperfusion.
OBJECTIVE
To investigate the apoptosis rules of the astrocytes and oligodendrocytes induced by Ca(2+) reperfusion.


METHODS
The apoptosis of purified cultured astrocytes and oligodendrocytes induced by Ca(2+) reperfusion and the relationship between the development of the cell apoptosis and post-reperfusion time was observed.


RESULTS
Both the astrocytes and oligodendrocytes were obviously in a time-dependent fashion, and the apoptosis ratios of the oligodendrocytes (39.73%+/-4.16%) were higher than the astrocytes (19.64%+/-4.67%) 24 hours after Ca(2+) reperfusion. The TUNEL positive cells were 13.6+/-1.82 and 21.4+/-1.95 at every visual field of astrocytes and oligodendrocytes respectively 24 hours after Ca(2+) reperfusion.


CONCLUSIONS
The astrocytes and oligodendrocytes are similar wi th the development rules on apoptosis and have different susceptiveness to the situation.
Ma
Haihan
H
Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China. yhchenmhh@163.net
Shao
Yang
Y
Wu
Yamin
Y
Li
Hongyun
H
Liao
Weihong
W
Liu
Yuan
Y
Yang
Hengwen
H
Zeng
Lin
L
Long
Zaiyun
Z
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001253:Astrocytes / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D009836:Oligodendroglia / Q000166:cytology* / Q000473:pathology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D002118:Calcium