title: Purification, crystallisation and X-ray diffraction study of fully functional laccases from two ligninolytic fungi.
abstract: Laccase isozymes from the white-rot basidiomycete fungi Trametes versicolor and Pycnoporus cinnabarinus were purified to apparent iso-electric homogeneity and crystallised. T. versicolor laccase crystallises in two crystal forms, both with the orthorhombic space group P2(1)2(1)2(1), which diffract to 1.9 and 2.95 A resolution, respectively. The crystals of P. cinnabarinus laccase belong to the monoclinic space group C2 and diffract to at least 2.2 A resolution. All the laccase crystals are suitable for X-ray structure determination and contain a full complement of copper ions.
authors.affiliation: Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), Universitätstrasse 16, CH-8092 Zürich, Switzerland.
authors.forename: Matteo
authors.initials: M
authors.lastname: Antorini
authors.forename: Isabelle
authors.initials: I
authors.lastname: Herpoël-Gimbert
authors.forename: Thomas
authors.initials: T
authors.lastname: Choinowski
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Sigoillot
authors.forename: Marcel
authors.initials: M
authors.lastname: Asther
authors.forename: Kaspar
authors.initials: K
authors.lastname: Winterhalter
authors.forename: Klaus
authors.initials: K
authors.lastname: Piontek
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003300:Copper / Q000737:chemistry; D003460:Crystallization; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification; D042845:Laccase; D008031:Lignin / Q000378:metabolism; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification*; D014961:X-Ray Diffraction
chemical_list: D007527:Isoenzymes; D003300:Copper; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase