A comprehensive karyotypic analysis on a newly established sarcomatoid hepatocellular carcinoma cell line SH-J1 by comparative genomic hybridization and chromosome painting.
We first established a sarcomatoid hepatocellular carcinoma cell line, designated as SH-J1, and applied comparative genomic hybridization and fluorescence in situ hybridization (FISH) with chromosome painting probes for the characterization of the chromosomal rearrangements. In the SH-J1 cell line, the pleomorphic spindle cells were arranged in bundles of interlacing patterns and were positive in immunohistochemical staining with hepatocyte-related markers. By G-banding and FISH, the chromosomal gains were detected at 6p and 17, whereas losses were observed at 3p21-pter, 3q27-qter, 4, 6q, 13pter-q11, 16, 18, 19p13, and Y.
Kim
Dae Ghon
DG
Research Institute of Clinical Medicine, Department of Internal Medicine, Chonbuk National University Medical School, Chonju, South Korea.
Park
Soo Yeun
SY
Kim
Hyun
H
Chun
Yong Hyuck
YH
Moon
Woo Sung
WS
Park
Sun Hwa
SH
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D020223:Chromosome Painting; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*; D014407:Tumor Cells, Cultured