[Effects of Repetitive Exposure to Hyperbaric Oxygen (HBO) on Leukocyte Function].
OBJECTIVE
Despite favourable clinical data on the successful use of hyperbaric oxygen (HBO), only limited investigations have been carried out to date regarding the influence of hyperoxia on leukocyte function. In a murine model, CD4+ T-cell population remained unchanged after repeated HBO exposure, however CD8+ cells were found to be increased. The aim of this study was to investigate whether repetitive exposure to hyperoxia would affect human monocyte and lymphocyte function.


METHODS
Methods: After Ethics Committee approval the effects of elevated partial oxygen pressure were studied in the course of a ten-day HBO therapy (2.5 atmospheres absolute over a daily period of 90 min). Monocytes and lymphocytes of 30 patients with acute hearing loss were determined by flow cytometry before, throughout and after HBO therapy using monoclonal antibodies to CD3, CD4, CD8, CD14, CD25, CD45 and HLA-DR. Statistical analysis was made by ANOVA (analysis of variance).


RESULTS
The relative percentage of CD3+, CD4+, CD8+, CD25+, CD14+, and HLA-DR+ cells remained unchanged during the course of and after HBO therapy.


CONCLUSIONS
We conclude that repetitive exposure to hyperoxia does not influence human monocyte and lymphocyte functions in contrast to experimental data.
Jaeger
K
K
Zentrum Anästhesiologie, Medizinische Hochschule Hannover. jaeger.karsten@mh-hannover.de
Jüttner
B
B
Sommer
C
C
Scheinichen
D
D
Ruschulte
H
H
Franko
W
W
Heine
J
J
D000328:Adult; D000911:Antibodies, Monoclonal; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D007962:Leukocytes / Q000187:drug effects*; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000187:drug effects
D000911:Antibodies, Monoclonal