Preparation of an active recombinant peptide of crustacean androgenic gland hormone.
In crustaceans, male sexual characteristics are induced by a hormone referred to as androgenic gland hormone. We have recently cloned a candidate cDNA in the terrestrial isopod Armadillidium vulgare. In order to prove that this cDNA encodes the hormone, recombinant single-chain precursor molecules consisting of B chain, C peptide and A chain were produced using both baculovirus and bacterial expression systems. Neither recombinant precursors showed activity. Digestion of only the precursor carrying a glycan moiety with lysyl endopeptidase gave a heterodimeric peptide with hormonal activity by removing a part of C peptide. These results indicate that the cDNA encodes the hormone.
Okuno
Atsuro
A
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan.
Hasegawa
Yuriko
Y
Nishiyama
Makoto
M
Ohira
Tsuyoshi
T
Ko
Rinkei
R
Kurihara
Masaaki
M
Matsumoto
Shogo
S
Nagasawa
Hiromichi
H
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003445:Crustacea / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
D004220:Disulfides; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones; D011134:Polysaccharides; D011994:Recombinant Proteins; C053644:androgenic gland hormone (Armadillidium)