KCO1 is a component of the slow-vacuolar (SV) ion channel.
The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels.
Schönknecht
Gerald
G
Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Molekulare Pfanzenphysiologie und Biophysik, Universität Würzburg, 97082 Würzburg, Germany.
Spoormaker
Petra
P
Steinmeyer
Ralf
R
Brüggeman
Lioubov
L
Ache
Peter
P
Dutta
Rajiv
R
Reintanz
Birgit
B
Godde
Matthias
M
Hedrich
Rainer
R
Palme
Klaus
K
D017360:Arabidopsis / Q000737:chemistry / Q000166:cytology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004553:Electric Conductivity; D017353:Gene Deletion; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D009928:Organ Specificity; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana; D014617:Vacuoles / Q000737:chemistry* / Q000378:metabolism
D029681:Arabidopsis Proteins; C503455:KCO1 protein, Arabidopsis; D008164:Luminescent Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D012333:RNA, Messenger; D018749:RNA, Plant; D049452:Green Fluorescent Proteins