title: A high-resolution reference map for cytoplasmic and membrane-associated proteins of Corynebacterium glutamicum.
abstract: We present a high-resolution reference map for soluble proteins obtained from Corynebacterium glutamicum cells grown in glucose minimal medium. The analysis window covers the pl range from 4-6 and the molecular mass range from 5-100 kDa. Using overlapping narrow immobilized pH gradients for isoelectric focusing, 970 protein spots were detected after second-dimensional separation on SDS-polyacrylamide gels and colloidal Coomassie-staining. By tryptic peptide mass fingerprinting 169 protein spots were identified, representing 152 different proteins including many enzymes involved in central metabolism (18), amino acid biosynthesis (24) and nucleotide biosynthesis (11). Thirty-five of the identified proteins have no known function. A comparison of the observed and the expected physicochemical properties of the identified proteins indicated that nine proteins were covalently modified, since variants with apparently identical molecular mass, but differing pl were detected. The N-termini of eight proteins were determined by post-source decay (PSD) analysis of selected peptides. In addition to the soluble proteins, a map of the membrane-bound proteins within the pl range 4-7 is presented, which contains 660 protein spots, 22 of which were identified, representing 13 different proteins.
authors.affiliation: Institut für Biotechnologie 1, Forschungszentrum Jülich, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schaffer
authors.forename: B
authors.initials: B
authors.lastname: Weil
authors.forename: V D
authors.initials: VD
authors.lastname: Nguyen
authors.forename: G
authors.initials: G
authors.lastname: Dongmann
authors.forename: K
authors.initials: K
authors.lastname: Günther
authors.forename: M
authors.initials: M
authors.lastname: Nickolaus
authors.forename: T
authors.initials: T
authors.lastname: Hermann
authors.forename: M
authors.initials: M
authors.lastname: Bott
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003352:Corynebacterium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins