Magneto-optical measurements of the pigments in fully active photosystem II core complexes from plants.
Preparation of a minimum PSII core complex from spinach is described, containing four Mn per reaction center (RC) and exhibiting high O2 evolving activity [approximately 4000 micromol of O2 (mg of chl)(-1) x h(-1)]. The complex consists of the CP47 and CP43 chlorophyll binding proteins, the RC D1/D2 pair, the cytochrome b559 subunits, and the Mn-stabilizing psbO (33 kDa) protein, all present in the same stoichiometric amounts found in the parent PSII membranes. Several small subunits are also present. The cyt b559 content is 1.0 per RC in core complexes and PSII membranes. The total chlorophyll content is 32 chl a and <1 chl b per RC, the lowest yet reported for any active PSII preparation. The core complex exhibits the characteristic EPR signals seen in the S2 state of higher plant PSII. A procedure for preparing low-temperature samples of very high optical quality is developed, allowing detailed optical studies in the S1 and S2 states of the system to be made. Optical absorption, CD, and MCD spectra reveal unprecedented detail, including a prominent, well-resolved feature at 683.5 nm (14630 cm(-1)) with a weaker partner at 187 cm(-1) to higher energy. On the basis of band intensity, CD, and MCD arguments, these features are identified as the exciton split components of P680 in an intact, active reaction center special pair. Comparisons are made with solubilized D1/D2/cyt b559 material and cyanobacterial PSII.
Smith
Paul J
PJ
Faculties Chemistry, Research School of Chemistry, and Research School of Biological Sciences, The Australian National University, Canberra, ACT 0200, Australia.
Peterson
Sindra
S
Masters
Vanessa M
VM
Wydrzynski
Tom
T
Styring
Stenbjörn
S
Krausz
Elmars
E
Pace
Ron J
RJ
D002942:Circular Dichroism; D003573:Cytochrome b Group / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D045342:Light-Harvesting Protein Complexes; D008280:Magnetics; D008345:Manganese / Q000737:chemistry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification; D045332:Photosystem II Protein Complex; D013053:Spectrophotometry; D018724:Spinacia oleracea / Q000737:chemistry
D003573:Cytochrome b Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D008345:Manganese; C019313:cytochrome b559