title: Proliferation in the posterior region of the lens of c-maf-/- mice.
abstract: OBJECTIVE
  To examine the involvement of the c-maf gene in the proliferation of the lens cells.
  
  
  METHODS
  Eyes of the E13 and E18 stages of the wild-type and c-maf-/- mice were analyzed by BrdU incorporation assay, TUNEL assay and immunocytochemistry using a anti-P27(KIP1) and a anti-P57(KIP2) antibody.
  
  
  RESULTS
  In the E13 and E18 c-maf mutant lens, BrdU-positive cells were detected at the posterior region of the lens. Cell-cycle inhibitor P27(KIP1) and P57(KIP2) were expressed in the equatorial and posterior region of the lens of both wild-type and c-maf-/- lenses.
  
  
  CONCLUSIONS
  These results suggest that the expression of c-maf is required for differentiation and cell cycle arrest of lens fiber cells. It is also suggested that P27(KIP1) and P57(KIP2) were not involved in the continued proliferation of posterior region of the c-maf-/- lens.
authors.affiliation: Department of Ophthalmology, Hokkaido University School of Medicine, Kita-ku, Sapporo, Japan. kyoshida@med.hokudai.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: J I
authors.initials: JI
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Imaki
authors.forename: I
authors.initials: I
authors.lastname: Hiromi
authors.forename: S
authors.initials: S
authors.lastname: Nishi
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: C
authors.initials: C
authors.lastname: Harada
authors.forename: S
authors.initials: S
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Sakai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27; D050761:Cyclin-Dependent Kinase Inhibitor p57; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000502:physiology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D020287:In Situ Nick-End Labeling; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000378:metabolism; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D051257:Proto-Oncogene Proteins c-maf; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: C493961:Cdkn1b protein, mouse; C493965:Cdkn1c protein, mouse; D018797:Cell Cycle Proteins; D050761:Cyclin-Dependent Kinase Inhibitor p57; D004268:DNA-Binding Proteins; C495608:Maf protein, mouse; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D051257:Proto-Oncogene Proteins c-maf; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004247:DNA; D001973:Bromodeoxyuridine