Rapid determination of caffeine in tea leaves.
For the purpose of efficient screening of low-caffeine tea shoots, a method for the rapid determination of caffeine was developed using high-performance liquid chromatography. Polyvinylpolypyrrolidone was packed in a pre-column and used to remove polyphenols from tea extracts on-line. The concentrations of caffeine extracted from powdered tea leaves at 50 degrees C during 1 day could be analyzed in 2-5 min intervals. The pre-column and the analytical column could be used for the analysis of more than 2000 samples.
Horie
Hideki
H
National Institute of Vegetable and Tea Science, Shizuoka, Japan. horie@affrc.go.jp
Nesumi
Atsushi
A
Ujihara
Tomomi
T
Kohata
Katsunori
K
D002110:Caffeine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry*; D011205:Povidone / Q000031:analogs & derivatives* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013662:Tea / Q000737:chemistry*
D013662:Tea; C077842:polyvinylpolypyrrolidone; D002110:Caffeine; D011205:Povidone