title: Kinetics of cytokine expression in mice with invasive aspergillosis: lethal infection and protection.
abstract: Aspergillus fumigatus causes lethal invasive aspergillosis in cortisone-treated mice while immunized mice are protected. Kinetics of interferon (IFN)-gamma, tumor necrosis factor-alpha, interleukin (IL)-12p40, IL-4 and IL-10 expression were assessed by reverse transcription-PCR in the spleen and lungs of mice. After immunization, mice were protected from otherwise lethal infection, with concomitant high expression levels of IFN-gamma early in the lungs and later in the spleen. In the lethal infection model cortisone-treated infected mice presented high expression levels of IL-10 in the spleen and in the lungs. Immunocompetent mice were able to control the disease and presented earlier expression of IL-12p40 and IFN-gamma in lungs and spleen. These results confirm the importance of Th1 dependent immunity against invasive aspergillosis.
authors.affiliation: Centro de Maláraia e Outras Doenças Tropicais/Instituto de Higiene e Medicina Tropical/Universidade Nova de Lisboa, Lisboa, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Centeno-Lima
authors.forename: Henrique
authors.initials: H
authors.lastname: Silveira
authors.forename: Catarina
authors.initials: C
authors.lastname: Casimiro
authors.forename: Pedro
authors.initials: P
authors.lastname: Aguiar
authors.forename: Virgílio E
authors.initials: VE
authors.lastname: do Rosário
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000276:immunology*; D001232:Aspergillus fumigatus / Q000276:immunology*; D016207:Cytokines / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D007114:Immunization; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D018664:Interleukin-12 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D007700:Kinetics; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma