title: [The detection and its clinical significance of cancer cells in portal vein blood of patients with colorectal carcinoma].
abstract: OBJECTIVE
  To study the relation between cancer cells in portal vein blood and liver metastasis of colorectal carcinoma.
  
  
  METHODS
  The presence of CK20 mRNA was investigated by non-isotope RT-PCR in 54 cases.
  
  
  RESULTS
  The positive rate of CK2O mRNA in portal vein blood was 75.9%. The positive cases were significantly correlated with Dukes stage and live metastasis. There was a higher probability of liver metastasis in positive cases after operation.
  
  
  CONCLUSIONS
  The detection of CK20 in portal vein blood may improve the accuracy of clinical staging and diagnose liver micrometastasis of colorectal carcinoma.
authors.affiliation: Department of General Surgery, Beijing General Hospital of People's Leberation Army, Beijing 100700.
authors.forename: C
authors.initials: C
authors.lastname: Luo
authors.forename: S
authors.initials: S
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015179:Colorectal Neoplasms / Q000097:blood / Q000473:pathology*; D005260:Female; D006801:Humans; D053554:Keratin-20; D007633:Keratins / Q000097:blood* / Q000235:genetics; D008113:Liver Neoplasms / Q000556:secondary; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011169:Portal Vein; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C507499:KRT20 protein, human; D053554:Keratin-20; D012333:RNA, Messenger; D007633:Keratins