[Surgical injuries influence on release of endothelin-1 and local blood perfusion of amputated extremities].
OBJECTIVE
To observe influence of amputations and surgical injuries on synthesis and release of endothelium-derived endothelin-1, and local blood perfusion of amputated extremities.


METHODS
The plasma endothelin-1 concentrations of 18 patients preoperatively and postoperatively and the endothelin-1 concentrations of femoral artery wall in 80 SD rats were determined using radioimmunoassay technique before and after amputation. Local blood perfusion of amputated extremities in rats were determined using laser Doppler flowmetry.


RESULTS
The plasma endothelin-1 concentrations in the patients with surgical injuries increased obviously, reached peak value at fourth hour postoperatively, being approximately 3 times of that of the control group. The endothelin-1 concentrations of femoral artery wall in amputated rats also increased, reached peak value at second hour after amputation, being approximately 2 times of that of the control group, and then decreased gradually, at the same time the local blood perfusion decreased, reached its lowest value at second hour after amputation, being only 25 percent of the control value. The endothelin-1 concentration of rat femoral artery wall was negatively relative to local blood perfusion (r = -0.83).


CONCLUSIONS
Injuries enhance synthesis and release of endothelin-1. It is probably one of the main causes of vasospasm after injuries.
Wang
T
T
Department of Hand Surgery, Huashan Hospital, Shanghai Medical University, Shanghai 200040.
Gu
Y
Y
Tan
W
W
Jin
H
H
D000293:Adolescent; D000328:Adult; D000671:Amputation, Surgical; D000818:Animals; D019332:Endothelin-1 / Q000097:blood*; D005260:Female; D005542:Forearm / Q000098:blood supply / Q000601:surgery*; D006614:Hindlimb / Q000098:blood supply / Q000601:surgery*; D006801:Humans; D017078:Laser-Doppler Flowmetry; D007866:Leg / Q000098:blood supply / Q000601:surgery*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
D019332:Endothelin-1