[Timing changes of apoptosis and proliferating cells nuclear antigen after intra-arterial infusion chemotherapy for rectal cancer].
OBJECTIVE
To investigate the timing changes of apoptosis (APO) and PCNA after intra-arterial infusion chemotherapy for rectal cancer.


METHODS
Twelve patients were subjected to percutaneous arterial femoralis catheterization by Seldinger's technique and infusion of anti-cancer drugs: 5-fluorouracil (5-Fu) 600 mg/m(2), mitomycin (MMC) C5 mg/m(2) and epirubicin (EDR) 35 mg/m(2). The biopsy of rectal tumor tissues was done before chemotherapy, and 24, 48, 72 hours and 7-10 days after chemotherapy. Apoptotic cells were examined by terminal-deoxynucleotidyl-transferase (TdT)-mediated dUTP-fluorescein and labeling. The expression of proliferating cells nuclear antigen (PCNA) was detected by immunohistologic staining.


RESULTS
The apoptosis index (AI) of rectal cancer cells before chemotherapy, and 24, 48, 72 hours, and 7-10 days after chemotherapy was 6.84 per thousand, 19.53 per thousand, 13.15 per thousand, 11.85 per thousand and 11.86 per thousand respectively. The PCNA index (PI) was 45.56%, 40.68%, 39. 87%, 51.28% and 63.75% before and 24, 48, 72 hours, and 7-10 days after chemotherapy.


CONCLUSIONS
Intra-arterial infusion chemotherapy not only induced apoptosis effectively, but also inhibited temporarily tumor cells proliferation in patients. The curative surgical treatment should be performed as soon as possible after chemotherapy.
Liu
F
F
Department of Surgery, Nanjing General Hospital, People's Liberation Army, Nanjing 210002.
Qi
X
X
Chen
X
X
Xu
Z
Z
Li
J
J
D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D017024:Chemotherapy, Adjuvant; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D007261:Infusions, Intra-Arterial; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D018809:Proliferating Cell Nuclear Antigen