Cl- channels in basolateral TAL membranes. XVI. MTAL and CTAL cells each contain the mRNAs encoding mmClC-Ka and mcClC-Ka.
BACKGROUND
Our prior data indicate that two separate but homologous basolateral chloride (Cl-) channels, mmClC-Ka and mcClC-Ka, are the principal mediators of net Cl- absorption in mouse medullary thick ascending limb (MTAL) and cortical thick ascending limb (CTAL) cells, respectively. In the present studies, we evaluated the possibility that there might be translational or post-translational suppression of mmClC-Ka and mcClC-Ka activity in CTAL and MTAL cells, respectively.


METHODS
Polymerase chain reaction (PCR) fragments were prepared that were highly specific for either mmClC-Ka or mcClC-Ka, the cDNAs encoding mmClC-Ka and mcClC-Ka, respectively.


RESULTS
Using reverse transcription (RT)-PCR with these highly specific products, mRNAs specific for non-homologous channel sequences in either mmClC-Ka or mcClC-Ka were present in both MTAL and CTAL cells.


CONCLUSIONS
Both mouse MTAL and CTAL cells contain the mRNAs encoding mmClC-Ka and mcClC-Ka. There may be translational or post-translational suppression of mmClC-Ka activity in CTAL cells, and of mcClC-Ka activity in MTAL cells.
Mikhailova
Marina V
MV
Division of Nephrology, Department of Internal Medicine, University of Arkansas College of Medicine, 4301 West Markham, Little Rock, AR 72205, USA.
Winters
Christopher J
CJ
Andreoli
Thomas E
TE
D000818:Animals; D002460:Cell Line; D018118:Chloride Channels / Q000378:metabolism*; D007672:Kidney Cortex; D007679:Kidney Medulla; D008138:Loop of Henle / Q000166:cytology / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D018118:Chloride Channels; D012333:RNA, Messenger