title: Effects of interleukin-8 and Helicobacter pylori on histamine release from isolated canine gastric mucosal mast cells.
abstract: BACKGROUND
  In recent studies, the involvement of mast cells in the pathogenesis of Helicobacter pylori infection was suggested. In the present study, using isolated canine gastric mucosal mast cells, we undertook to elucidate the effects of interleukin-8 (IL-8) and H. pylori on histamine release from these cells.
  
  
  METHODS
  Enriched canine gastric mucosal mast cells (50% target cells) were incubated in Hanks medium with IL-8, or water extract or sonicate of H. pylori for 15 min at 37 degrees C. The content of histamine in the supernatants and the cell pellets after centrifugation was assayed with a histamine radioimmunoassay (RIA) kit.
  
  
  RESULTS
  IL-8 (50 ng/ml) and concanavalin A (20 microg/ml) significantly increased histamine release from enriched gastric mucosal mast cells. Dose-dependent stimulation of histamine release by IL-8 (5-50 ng/ml) was also seen. Water extract and sonicate of H. pylori (10(8) bacteria) increased histamine release from mast cells. A concentration-dependent stimulation of histamine release by water extract or sonicate was also seen. The maximal response of histamine release was seen at the highest concentration of the water extract or sonicate.
  
  
  CONCLUSIONS
  The results indicated that IL-8 and H. pylori had stimulatory effects on histamine release from canine gastric mucosal mast cells. The results imply that IL-8 and soluble factors of H. pylori may accelerate inflammation of the gastric mucosa via histamine release from mast cells.
authors.affiliation: Third Department of Internal Medicine, Teikyo University Ichihara Hospital, Anesaki, Ichihara, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yakabi
authors.forename: Takashi
authors.initials: T
authors.lastname: Arimura
authors.forename: Mitsuhito
authors.initials: M
authors.lastname: Koyanagi
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Uehigashi
authors.forename: Shoki
authors.initials: S
authors.lastname: Ro
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minagawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004285:Dogs; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D016481:Helicobacter Infections / Q000503:physiopathology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D006636:Histamine Release / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D013272:Stomach Diseases / Q000382:microbiology / Q000503:physiopathology
chemical_list: D001426:Bacterial Proteins; D016209:Interleukin-8; D003208:Concanavalin A