title: Peptide-protein interactions studied by surface plasmon and nuclear magnetic resonances.
abstract: The structural features of the complexes that alpha-bungarotoxin forms with three different synthetic peptides, mimotopes of the nicotinic acetylcholine receptor binding site, have been compared to the corresponding nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) data. For the considered peptides, the observed different affinities towards the toxin could not be accounted simply by static structural considerations. A combined analysis of the SPR- and NMR-derived dynamic parameters shows new correlations between complex formation and dissociation and the overall pattern of intramolecular and intermolecular nuclear Overhauser effects. These features could be crucial for a rational design of protein ligands.
authors.affiliation: Biomolecular Structure Research Center and Department of Molecular Biology, University of Siena, Siena, Italy.
authors.forename: Ottavia
authors.initials: O
authors.lastname: Spiga
authors.forename: Andrea
authors.initials: A
authors.lastname: Bernini
authors.forename: Maria
authors.initials: M
authors.lastname: Scarselli
authors.forename: Arianna
authors.initials: A
authors.lastname: Ciutti
authors.forename: Luisa
authors.initials: L
authors.lastname: Bracci
authors.forename: Luisa
authors.initials: L
authors.lastname: Lozzi
authors.forename: Barbara
authors.initials: B
authors.lastname: Lelli
authors.forename: Daniela
authors.initials: D
authors.lastname: Di Maro
authors.forename: Duccio
authors.initials: D
authors.lastname: Calamandrei
authors.forename: Neri
authors.initials: N
authors.lastname: Niccolai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002038:Bungarotoxins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D018716:Molecular Mimicry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D002038:Bungarotoxins; D010455:Peptides; D011978:Receptors, Nicotinic