[Neurite-stimulating activity of secretions from the Hirudo medicinalis salivary gland in cultured sensory neurons].
Components of leech (Hirudo medicinalis) salivary gland secretion (high molecular mass bdelin B, bdellastasin, eglin C, destabilize) were used to investigate their neurotrophic effect on organotypic culture of dorsal root ganglia of the 10-11-day old chicken embryos. Destabilize and high molecular mass bdelin B in concentrations of 0.01, 0.02, 0.05 and 0.1 ng/ml, bdellastasin in concentrations of 0.02 and 0.05 ng/ml, eglin C in concentrations of 0.1 ng/ml exhibit neurite-stimulating effect as compared to the control.
Chalisova
N I
NI
I. P. Pavlov Institute of Physiology, Russian Acad. Sci., St. Petersburg, 199034, Nab. Makarova, 6, Russia.
Pennijajnen
V P
VP
Baskova
I P
IP
Zavalova
L L
LL
Bazanova
A V
AV
D000818:Animals; D002642:Chick Embryo; D010450:Endopeptidases / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000648:ultrastructure; D019476:Insect Proteins; D007865:Leeches; D008970:Molecular Weight; D016501:Neurites / Q000187:drug effects*; D009475:Neurons, Afferent / Q000187:drug effects* / Q000648:ultrastructure; D009930:Organic Chemicals; D011480:Protease Inhibitors / Q000737:chemistry / Q000494:pharmacology; D011506:Proteins; D012469:Salivary Glands / Q000737:chemistry; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D015843:Serpins / Q000494:pharmacology
D019476:Insect Proteins; D009930:Organic Chemicals; D011480:Protease Inhibitors; D011506:Proteins; D015842:Serine Proteinase Inhibitors; D015843:Serpins; C400221:bdellastasin; C028656:eglin proteinase inhibitors; C107927:bdellin; D010450:Endopeptidases; C045348:fibrin destabilase