Lab-on-a-chip: applications in proteomics.
Recent advances in chip-based separation of proteins provide methods that are faster and more convenient than conventional gel electrophoresis. Rapid and automated protein sizing on a chip is at the commercial stage and first attempts have been made to perform two-dimensional separation on a chip. Numerous designs have been described to interface a microfluidic chip to a mass spectrometer. Impressive integration efforts are demonstrated by the ability to perform on-chip trypsin digestion, separation and injection into a mass spectrometer with a single device.
Mouradian
Stephane
S
Caliper Technologies Corp., 605 Fairchild Drive, Mountain View, California 94043, USA. stephane.mouradian@calipertech.com
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes; D006801:Humans; D008832:Microchemistry; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis*
D005456:Fluorescent Dyes; D011506:Proteins; D020543:Proteome