Identification and sequencing of the HLA-B*4106 allele.
We report a novel HLA-B*41 allele (HLA-B*4106) initially detected by an unusual sequence-specific oligonucleotide (SSO) hybridization pattern and identified by sequence-based HLA typing. Molecular cloning and sequencing determined that the new HLA-B allele was identical to the HLA-B*4101 in exon 2 and 3 except for a single nucleotide substitution in the exon 3 changing codon 204 from Glu to Gln (GAG-->CAG). In addition, intron 2 was identical to the published HLA-B*4104 intron 2, except for the base 509 (C-->G).
Vidal
S
S
Department of Immunology, Hospital Sant Pau and Inst. Rec. Sant Pau, Barcelona, Spain. Svidal@hsp.santpau.es
Morante
M P
MP
Mosquera
A M
AM
Moga
E
E
Querol
S
S
García
J
J
Rodríguez-Sánchez
J L
JL
D000483:Alleles; D001483:Base Sequence; D005312:Fetal Blood; D015235:HLA-B Antigens / Q000032:analysis* / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens