Detection of IL-2 receptor gene expression in peripheral blood from renal transplant patients.
Cytokine monitoring is a potentially useful tool in the management of transplant patients. This study was conducted to analyze interleukin (IL)-1, tumor necrosis factor (TNF), IL-2, and IL-2 receptor gene expression by reverse transcriptase-polymerase chain reaction (RT-PCR) in peripheral whole blood from healthy volunteers and patients who underwent laparotomy or renal transplantation. In the patients who underwent laparotomy, IL-1 and TNF expression was detected postoperatively, but IL-2 and IL-2 receptor expression was not. IL-1, TNF, and IL-2 receptor gene expression was detected in all the renal transplant patients, including those with signs of rejection. No cytokine gene expression was detected preoperatively, pretransplant, or in the healthy volunteers. We were able to discriminate transplantation from laparotomy by the cytokine profile. These results indicate that our method may be a useful tool for the immunological monitoring of transplant patients.
Shimizu
S
S
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
Ueda
M
M
Ozawa
S
S
Wakabayashi
G
G
Endo
M
M
Hayakawa
K
K
Hata
M
M
Murai
M
M
Kitajima
M
M
D000328:Adult; D000368:Aged; D016207:Cytokines / Q000097:blood*; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D007813:Laparotomy; D008297:Male; D008875:Middle Aged; D015166:Monitoring, Immunologic / Q000379:methods*; D015375:Receptors, Interleukin-2 / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D015375:Receptors, Interleukin-2