Pigeon pea waste as a novel, inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp. JB-99.
Thermoalkaliphilic Bacillus sp. JB-99 was grown in a 250 ml Erlenmeyer flask containing 50 ml medium containing (g/l) Pigeon pea waste 10; NaNO3, 5.0; K2HPO4, 5.0; MgSO4 x 2H2O, 0.2 and Na2CO3, 10.0. Incubations were carried out at 50 degrees C on a rotary incubator shaker for 15 h. A high level of extra cellular thermostable protease activity was observed after 24 h incubation. The optimum temperature and pH for activity were 70 degrees C and 11, respectively, so this enzyme showed stable activity at high temperature and under alkaline conditions.
Johnvesly
B
B
Department of Biotechnology, Gulbarga University, India.
Manjunath
B R
BR
Naik
G R
GR
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D005110:Extracellular Space / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000493:pharmacokinetics / Q000494:pharmacology; D027805:Phaseolus / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D004791:Enzyme Inhibitors; D008670:Metals; D012965:Sodium Chloride; D012697:Serine Endopeptidases; C032923:microbial serine proteinases