title: Discrimination of single-copy IS6110 DNA fingerprints of Mycobacterium tuberculosis isolates by high-resolution minisatellite-based typing.
abstract: Seven isoniazid-resistant isolates with mutations in the NADH dehydrogenase (ndh) gene were molecularly typed by IS6110-based restriction fragment length polymorphism analysis. All seven isolates with the R268H mutation had identical 1.4-kb IS6110 fingerprints. High-resolution minisatellite-based typing discriminated five of these isolates; two isolates were identical.
authors.affiliation: Department of Clinical Research, Singapore General Hospital, Singapore 169608. dmslsg@nccs.com.sg
authors.forename: Ann S G
authors.initials: AS
authors.lastname: Lee
authors.forename: Lynn L H
authors.initials: LL
authors.lastname: Tang
authors.forename: Irene H K
authors.initials: IH
authors.lastname: Lim
authors.forename: Richard
authors.initials: R
authors.lastname: Bellamy
authors.forename: Sin-Yew
authors.initials: SY
authors.lastname: Wong
mesh_terms: D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004251:DNA Transposable Elements; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements