title: [E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma].
abstract: OBJECTIVE
  To study the significance of E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  Flow cytometry and immunohistochemistry were used to detect the expression of E-selectin and its ligand-sLeX in HCC cell lines and in human HCC tissues.
  
  
  RESULTS
  The positive rate of E-selectin in vascular endothelial cells adjacent to cancer nest in tumors was 67.9% (19/28). In tumors accompanied with emboli or satellite foci, it was significantly higher than that without emboli or satellite foci (P < 0.05). The positive rate of E-selectin was not related to tumor size, tumor capsule, AFP, and the degree of differentiation. The positive expressions of sLeX in SMMU-7721, PLC/PRF/5 and HepGII cell lines were 7.03%, 63.35% and 97.29% respectively. The positive cells of sLeX were mainly distributed in the margin of tumors; the positive expression of sLeX in HCC cells in emboli or invasive tumor tissues was much higher than that in primary foci.
  
  
  CONCLUSIONS
  E-selectin and its ligand-sLeX are closely correlated with the metastasis of HCC.
authors.affiliation: Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, People's Liberation Army, Shanghai 200438, China.
authors.forename: B
authors.initials: B
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.forename: W
authors.initials: W
authors.lastname: Cong
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D019040:E-Selectin / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009844:Oligosaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
chemical_list: D019040:E-Selectin; D008024:Ligands; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen