The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance.
Plant disease resistance (R) genes trigger innate immune responses upon pathogen attack. RAR1 is an early convergence point in a signaling pathway engaged by multiple R genes. Here, we show that RAR1 interacts with plant orthologs of the yeast protein SGT1, an essential regulator in the cell cycle. Silencing the barley gene Sgt1 reveals its role in R gene-triggered, Rar1-dependent disease resistance. SGT1 associates with SKP1 and CUL1, subunits of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex. Furthermore, the RAR1-SGT1 complex also interacts with two COP9 signalosome components. The interactions among RAR1, SGT1, SCF, and signalosome subunits indicate a link between disease resistance and ubiquitination.
Azevedo
Cristina
C
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
Sadanandom
Ari
A
Kitagawa
Katsumi
K
Freialdenhoven
Andreas
A
Shirasu
Ken
K
Schulze-Lefert
Paul
P
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000075686:COP9 Signalosome Complex; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D005800:Genes, Fungal; D017343:Genes, Plant; D001467:Hordeum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D010447:Peptide Hydrolases; D010453:Peptide Synthases / Q000378:metabolism; D010935:Plant Diseases; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D044843:SKP Cullin F-Box Protein Ligases; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism
D029681:Arabidopsis Proteins; C401415:COP8 protein, Arabidopsis; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; C430705:MLA6 protein, Hordeum vulgare; D046912:Multiprotein Complexes; C499581:PBS2 protein, Arabidopsis; D010940:Plant Proteins; D011506:Proteins; C499582:Rar1 protein, Hordeum vulgare; D011993:Recombinant Fusion Proteins; C452191:SGT1a protein, Arabidopsis; C452192:SGT1b protein, Arabidopsis; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D044843:SKP Cullin F-Box Protein Ligases; D010447:Peptide Hydrolases; D000075686:COP9 Signalosome Complex; D010453:Peptide Synthases