title: Structure determination of the constitutive 20S proteasome from bovine liver at 2.75 A resolution.
abstract: The crystal structure of the 20S proteasome from bovine liver was determined by the molecular replacement method using the structure of the 20S proteasome from the yeast Sacccharomyces cerevisiae. The initial phases were refined by density modification coupled with non-crystallographic symmetry averaging. The structural model was refined with the program CNS. The final R-factor and R(free) were 0.25 and 0.29, respectively. The constitutive proteasome without any contamination by the immunoproteasome was identified in the crystal structure.
authors.affiliation: Institute for Protein Research, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Unno
authors.forename: Tsunehiro
authors.initials: T
authors.lastname: Mizushima
authors.forename: Yukio
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tomisugi
authors.forename: Keiji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Noritake
authors.initials: N
authors.lastname: Yasuoka
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004563:Electrochemistry; D008099:Liver / Q000201:enzymology*; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex