[Responses of normal rat kidney to different ischemic ways].
OBJECTIVE
To evaluate the effects of different ischemic ways on rat kidneys.


METHODS
Male Wistar rat kidneys were divided into sham operation (group A), continuous ischemia (group B), intermittent ischemia (group C), ischemic preconditioning (group D), and delayed effect of ischemic preconditioning group (group E). The functional and morphologic changes and levels of MDA of the kidneys which suffered from different ischemia were assessed.


RESULTS
The functional indexes of the group C were similar to that of the sham group. In the group B, D and E, the rats showed acute renal failure. Morphological findings showed that the groups B, D and E had severe tubular necrosis (+ + + - + + + +) 24 hours after operation. The group C showed light tubular necrosis (+). 30 minutes after reperfusion, the level of MDA was significantly lower in the group C than in the groups B, D and E (P < 0.01).


CONCLUSIONS
In a given ischemic period, the injury effect of intermittent ischemia-reperfusion was much lower than that of continuous ischemia-reperfusion. Experimentally, ischemic preconditioning did not have protective effect on rat kidneys, including "classic protection" and "protection of second window". Intermittent ischemia-reperfusion did not increase OFR and lipid peroxidation injury.
Yu
Z
Z
Department of Vascular Surgery, Peking Union Medical College Hospital, Beijing 100730.
Wu
Y
Y
Guan
H
H
D000818:Animals; D007511:Ischemia / Q000150:complications; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply*; D007683:Kidney Tubular Necrosis, Acute / Q000209:etiology; D007684:Kidney Tubules / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology
D008315:Malondialdehyde