[An experimental investigation of inhibition of interferon on lens epithelial cell growth in vitro].
OBJECTIVE
To investigate the inhibition of alpha-interferon (alpha-IFN) and gamma-interferon (gamma-IFN) on rabbit lens epithelial cell (RLEC) proliferation in vitro and their effective concentrations.


METHODS
The second and third passage of RLEC were used for assay. Various concentrations of alpha-IFN or gamma-IFN were added into the culture medium and RLECs were exposed to these drugs. After 24 and 72 hours, the inhibition of RLECs was determined by counting the RLEC numbers on a counting plate and MTT colorimetric assay.


RESULTS
Alpha-IFN and gamma-IFN may reduce the proliferation of RLECs at the concentration of 10(3) - 10(4) IU/ml. The inhibition of gamma-IFN was a little stronger than that of alpha-IFN.


CONCLUSIONS
The experiment provides a scientific basis for selection of drugs to prevent after cataract.
Hu
N
N
Department of Ophthalmology, The Affiliated Hospital of Nantong Medical College, Nantong 226001.
Qiu
X
X
Gong
Q
Q
Guan
H
H
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology* / Q000187:drug effects; D011817:Rabbits; D011994:Recombinant Proteins
D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma