A gene encoding a protein modified by the phytohormone indoleacetic acid.
We show that the expression of an indole-3-acetic acid (IAA)-modified protein from bean seed, IAP1, is correlated to the developmental period of rapid growth during seed development. Moreover, this protein undergoes rapid degradation during germination. The gene for IAP1, the most abundant protein covalently modified by IAA (iap1, GenBank accession no. ) was isolated and cloned from bush bean (Phaseolus vulgaris) seeds. The 957-bp sequence encodes a 35-kDa polypeptide. IAA-modified proteins represent a distinct class of conjugated phytohormones and appear in bean to be the major form of auxin in seeds. IAA proteins also are found at other stages of development in bean plants. Our immunological and analytical data suggest that auxin modification of a small class of proteins may be a feature common to many plants.
Walz
Alexander
A
Department of Horticultural Science, University of Minnesota, Saint Paul, MN 55108, USA.
Park
Seijin
S
Slovin
Janet P
JP
Ludwig-Müller
Jutta
J
Momonoki
Yoshie S
YS
Cohen
Jerry D
JD
D000595:Amino Acid Sequence; D003227:Conjugation, Genetic; D017931:DNA Primers; D007887:Fabaceae / Q000235:genetics; D015723:Gene Library; D015151:Immunoblotting; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011994:Recombinant Proteins; C030737:indoleacetic acid