Parentage analysis for three Romanian families by DNA-fingerprinting.
In forensic medicine, DNA fingerprinting for human identification and paternity testing is becoming a necessary procedure. The genetic locus D1S80 (MCT118) with Hinf I polymorphism of its 5' flanking sequence, HUMTH01 and D21S11 have been successfully amplified from human genomic DNA isolated from blood (50 ng from each sample) by the polymerase chain reaction (PCR) using oligonucleotide primers complementary to the flanking sequences as primers for amplification. DNA bands were detected by ethidium bromide staining after electrophoresis on agarose gels or high-resolution SDS-PAGE. Analysis of these VNTR loci was thus achieved without the need for Southern blot or radioactive material. The small size of the DNA fragments produced in the PCR amplification permitted good resolution of individual alleles. The precise specification of the number of tandem repeats present in each allelic fragment was reproducible from one analysis to another. The aim of this study includes three paternity testing cases; they are the first three human DNA-fingerprints performed in Romania.
Ceacareanu
A C
AC
Ceacareanu
B
B
Ofiteru
A
A
Szegli
G
G
D020121:5' Untranslated Regions / Q000235:genetics; D000483:Alleles; D002648:Child; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D010334:Paternity; D012150:Polymorphism, Restriction Fragment Length; D012383:Romania
D020121:5' Untranslated Regions; D004247:DNA