Early progression from dimethyl sulfoxide-induced G(0)/G(1) arrest in L(1210) cells.
Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G(0)/G(1) arrest in mouse leukemia L(1210) cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA-synthesizing enzymes thymidylate synthase and thymidine kinase within 6h, and the cells entering into S phase within 12h. The sequence was followed by the marked activation of both enzymes within 24h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36 h. In conclusion, mouse leukemia L(1210) cells, which were treated with 1.5% DMSO for 96 h, tolerated the treatment and reversed the cell cycle arrest within 36 h.
Kudo
Hideki
H
Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510.
Nakayama
Tohru
T
Mano
Yoshihiro
Y
Suzuki
Satoe
S
Sassa
Shuji
S
Sakamoto
Shinobu
S
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects*; D007150:Immunohistochemistry; D051379:Mice; D012313:RNA / Q000378:metabolism; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D013937:Thymidine Kinase / Q000378:metabolism; D013940:Thymidylate Synthase / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D012313:RNA; D004247:DNA; D013940:Thymidylate Synthase; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine; D004121:Dimethyl Sulfoxide