title: ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation.
abstract: Data-dependent external m/z selection and accumulation of ions is demonstrated in use with ESI-FTICR instrumentation, with two different methods for ion selection being explored. One method uses RF/DC quadrupole filtering and is described in use with an 11.5 tesla (T) FTICR instrument, while the second method employs RF-only resonance dipolar excitation selection and is described in use with a 3.5 T FTICR instrument. In both methods ions are data-dependently selected on the fly in a linear quadrupole ion guide, then accumulated in a second linear RF-only quadrupole trap that immediately follows. A major benefit of ion preselection prior to external accumulation is the enhancement of ion populations for low-level species. This development is expected to expand the dynamic range and sensitivity of FTICR for applications including analysis of complex polypeptide mixtures (e.g., proteomics).
authors.affiliation: The Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Harkewicz
authors.forename: Mikhail E
authors.initials: ME
authors.lastname: Belov
authors.forename: Gordon A
authors.initials: GA
authors.lastname: Anderson
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Pasa-Tolić
authors.forename: Christophe D
authors.initials: CD
authors.lastname: Masselon
authors.forename: David C
authors.initials: DC
authors.lastname: Prior
authors.forename: Harold R
authors.initials: HR
authors.lastname: Udseth
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith