Toxicity monitoring and classification of endocrine disrupting chemicals (EDCs) using recombinant bioluminescent bacteria.
A recombinant bioluminescent Escherichia coli, DPD2794, containing the recA promoter region fused to luxCDABE originating from Vibrio fischeri was used for detecting genotoxicity caused by endocrine disrupting chemicals (EDCs) to cells. As well, several other recombinant bioluminescent bacteria, including TV1061, which is sensitive to protein damage (grpE::luxCDA BE), DPD2511, sensitive to oxidative damage (katG::luxCDABE), and DPD2540, sensitive to membrane damage (fabA::luxCDABE), were used for evaluating other possible modes of toxicity. It was found that the recombinant bacteria could monitor the toxic effect, not estrogenic effect, due to the presence of various EDCs through the measurement of bioluminescence (BL) and cell growth rate, which depend upon the type of toxicity occurring and which of the four strains was used. In addition, it was found that the damage caused by EDCs can be classified into several groups upon their mechanisms of toxic action.
Gu
Man Bock
MB
Department of Environmental Science Engineering, ADvanced Environmental Monitoring Research Center, Kwangju Institute of Science and Technology (K-JIST), South Korea. mbgu@kjist.ac.kr
Min
Jiho
J
Kim
Eun Jin
EJ
D001426:Bacterial Proteins / Q000235:genetics*; D002462:Cell Membrane; D004703:Endocrine System / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D008163:Luminescent Measurements; D009152:Mutagenicity Tests / Q000379:methods; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic / Q000235:genetics; D014733:Vibrio / Q000187:drug effects* / Q000502:physiology; D015262:Xenobiotics / Q000009:adverse effects*
D001426:Bacterial Proteins; D015262:Xenobiotics