WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35.
WW domains mediate protein-protein interactions in many intracellular processes. In pre-mRNA splicing, WW domains participate in cross-intron bridging. These WW domains are characterized by a central aromatic block of three tyrosine residues. We identified a novel protein containing the same type of WW domain. The gene encoding the protein, named WAC, is located in human chromosome 10p11.2-10p12.1. A Drosophila melanogaster WAC homolog (CG8949) was identified as a Rosetta stone protein. Domain fusion analysis of the Rosetta stone protein linked WAC to splicing factor SNRP70. WAC existed mainly in a tyrosine-phosphorylated form. Immunofluorescence analysis colocalized WAC with SC35, the marker for pre-mRNA splicing machinery. Our analysis suggests that WAC represents a novel member of WW-domain-containing proteins for RNA processing.
Xu
G Mark
GM
Polycystic Kidney Disease Program, Renal Unit, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA. gxu@helix.mgh.harvard.edu
Arnaout
M Amin
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D007690:Polycystic Kidney Diseases / Q000235:genetics; D017433:Protein Structure, Secondary / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012261:Ribonucleoproteins; D020539:Sequence Analysis, Protein; D000068103:Serine-Arginine Splicing Factors; D050396:TRPP Cation Channels
D009687:Nuclear Proteins; D011506:Proteins; D012261:Ribonucleoproteins; D000068103:Serine-Arginine Splicing Factors; D050396:TRPP Cation Channels; C499372:SRSF2 protein, mouse; C073288:SRSF2 protein, human