Anterior capsule dyes and labeled viscoelastic solutions to enhance contrast in EAS-1000 Scheimpflug images.
OBJECTIVE
To enhance contrast in images produced by the EAS-1000 to facilitate evaluation of the rheological properties of viscoelastic substances during simulated cataract surgery.


METHODS
Department of Preclinical Ophthalmology, Scientific Affairs, Pharmacia AB, Uppsala, Sweden.


METHODS
Cataract surgery was performed in 200 fresh porcine cadaver eyes and documented by EAS-1000 Scheimpflug photography. The light scattering caused by anterior capsule dyes, labeled irrigation solutions, and labeled viscoelastic solutions was statistically analyzed in the images.


RESULTS
Visualization by light scattering was significantly enhanced by a fluorescein labeled viscoelastic solution and by an eosin-Y- or phloxine-B-dyed capsule (P < .05). A side effect of eosin Y and phloxine B was that the capsule became fragile, making it difficult to perform a continuous curvilinear capsulorhexis.


CONCLUSIONS
Fluorescein-labeled viscoelastic solutions are recommended to enhance contrast in the images when retention capacity is evaluated. Light scattering from capsule dyeing facilitated anterior chamber depth measurements in the images.
Holmén
Jörgen B
JB
Department of Preclinical Ophthalmology, Scientific Affairs, Pharmacia AB, Uppsala, Sweden. jorgen.holmen@kirmed.slu.se
D000818:Animals; D000867:Anterior Chamber / Q000033:anatomy & histology; D004396:Coloring Agents; D003287:Contrast Media; D004338:Drug Combinations; D006820:Hyaluronic Acid; D007903:Lens Capsule, Crystalline / Q000033:anatomy & histology*; D018918:Phacoemulsification; D010781:Photography / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D013552:Swine
D004396:Coloring Agents; D003287:Contrast Media; D004338:Drug Combinations; D006820:Hyaluronic Acid