title: Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray.
abstract: Genes differentially expressed between parthenogenetic and androgenetic embryos are candidates for the identification of imprinted genes, which are expressed specifically from the maternal or paternal allele. To search for genes differentially expressed between parthenogenetic and androgenetic embryos, we used the RIKEN full-length enriched mouse cDNA microarray. The 25 candidates obtained included 8 known imprinted genes (such as IgfII, Snrpn, and Neuronatin) and 3 new ones--Asb4 (ankyrin repeat and SOCS box-containing protein 4), Ata3 (amino acid transport system A3), and Decorin--which were confirmed by using normal diploid embryos from the reciprocal F1 crosses of B6 and JF1 mice. The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray.
authors.affiliation: Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230-0045, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sotomaru
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Katsuzawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kono
authors.forename: Makiko
authors.initials: M
authors.lastname: Meguro
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomaru
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kiyosawa
authors.forename: Itoshi
authors.initials: I
authors.lastname: Nikaido
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Amanuma
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D026924:Amino Acid Transport System A / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics*; D001324:Autoantigens / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D058575:Decorin; D004622:Embryo, Mammalian; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D005820:Genetic Testing / Q000379:methods*; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
chemical_list: D026924:Amino Acid Transport System A; D001324:Autoantigens; D002352:Carrier Proteins; C546879:Dcn protein, mouse; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; D011509:Proteoglycans; D017411:Ribonucleoproteins, Small Nuclear; C420522:Slc38a4 protein, mouse; C486010:Slc38a4 protein, rat; D055517:snRNP Core Proteins; D007335:Insulin-Like Growth Factor II