In vitro regeneration of Hypericum patulum Thunb.--a medicinal plant.
A protocol was developed for high frequency plant regeneration in H. patulum by shoot-tip culture. H. patulum plants were collected from a wild source growing at high altitude in the eastern Himalayas. Multiple buds were initiated from shoot-tips cultured on Murashige and Skoog's medium supplemented with BAP, kinetin. Addition of thiamin HCI, Ca-pantothenate and biotin enhanced multiple shoot formation. Upon transfer to phytohormone free liquid medium following a brief exposure to auxin, root formation occurred from the micro shoots . Rooted plants were hardened and transferred to soil. Regeneration potentiality was found to be constant throughout the year in long term cultures.
Baruah
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Plant Sciences and Ecology Division, Regional Research Laboratory, Jorhat, India. ipmd@vsnl.net
Sarma
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Saud
J
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Singh
R S
RS
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001710:Biotin / Q000378:metabolism; D003470:Culture Media; D020902:Hypericum / Q000502:physiology*; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D010205:Pantothenic Acid / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D010946:Plants, Medicinal / Q000502:physiology*; D011687:Purines / Q000494:pharmacology; D012038:Regeneration / Q000502:physiology*; D013831:Thiamine / Q000378:metabolism
D003470:Culture Media; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D010205:Pantothenic Acid; D001710:Biotin; D000225:Adenine; D007701:Kinetin; D013831:Thiamine