title: Peanut and Amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes.
abstract: Lectins are relevant tools to isolate and characterize different cellular sub-populations. In this work, we used the lectins Arachis hypogaea (Peanut agglutinin, PNA) and Amaranthus leucocarpus (ALL), specific for Galss1, 3GalNAc, to characterize naive and memory lymphocytes from pigs, experimentally infected with the porcine rubulavirus (RvP). Our results showed that both lectins recognized preferentially lymphocytes with the CD4(+)CD8(+) phenotype (P<0.05). The phenotypic analysis of the cells recognized by these lectins indicated that PNA(+) lymphocytes showed higher rate of the CD29 antigen (PNA(+)CD29(high)) than ALL(+) (ALL(+)CD29(low)). The number of PNA(+)CD29(high) lymphocytes increased after 8 weeks of experimental infection with RvP, and most of the ALL(+)CD29(low) cells became CD29(middle). PNA(+) lymphocytes isolated from infected pigs proliferated after stimulation with the RvP, whereas ALL(+) cells did not. In vitro assays indicated that the ALL(+) cells from previously infected pigs diminished from 7.5 +/- 2 to 0.5 +/- 0.3% after RvP stimulation; whereas PNA(+) cells increased from 4 +/- 1 to 42 +/- 2%, whereas no modification in ALL(+) or PNA(+) cellular population was identified in lymphocytes from naive animals after RvP stimulation. Our results suggest that the cellular distribution/organization of the O-glycosydically linked glycans on lymphocytes may correlate with biological functions, and that PNA could be a tool to isolate specifically porcine memory T cell subsets, whereas ALL could be useful to isolate naive/quiescent T lymphocytes.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 14080, Tlalpan, Mexico. jhdez@cascabel.ciad.mx
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.forename: Yonathan
authors.initials: Y
authors.lastname: Garfias
authors.forename: Julio
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: Raúl
authors.initials: R
authors.lastname: Chávez
authors.forename: Ricardo
authors.initials: R
authors.lastname: Lascurain
authors.forename: Javier
authors.initials: J
authors.lastname: Vargas
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006031:Glycosylation; D007156:Immunologic Memory; D037102:Lectins / Q000276:immunology*; D019887:Peanut Agglutinin / Q000276:immunology*; D037121:Plant Lectins; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: C121359:Amaranthus leucocarpus lectin; D000956:Antigens, Viral; D006023:Glycoproteins; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins