Typing of Pasteurella multocida isolated from pigs with and without porcine dermatitis and nephropathy syndrome.
Porcine dermatitis and nephropathy syndrome (PDNS) is a sporadic, usually fatal disease of growing and finishing pigs that has been recognized in many pig-producing countries. Pasteurella multocida strains isolated from 15 pigs with PDNS and 51 pigs without PDNS were characterized by capsule and somatic antigen typing, random amplified polymorphic DNA (RAP-D) typing, and restriction analysis of genomic DNA using pulsed-field gel electrophoresis (PFGE). While capsular, somatic, and RAP-D typing did not discriminate PDNS isolates from non-PDNS isolates, all of the isolates from PDNS cases showed an identical ApaI PFGE restriction pattern. This pattern was also found in a high proportion (36%) of P. multocida strains isolated from non-PDNS cases. Isolation of a single variant of P. multocida from tissues of pigs with PDNS warrants further investigation into the possible role of these bacteria in the etiology of the disease.
Lainson
F A
FA
Moredun Research Institute, Pentland Science Park, Bush Loan, Edinburgh, EH26 0PZ, Scotland. laina@mri.sari.ac.uk
Aitchison
K D
KD
Donachie
W
W
Thomson
J R
JR
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology*
D000942:Antigens, Bacterial