[Measurement of tumor angiogenesis in primary lung cancer].
OBJECTIVE
To study tumor angiogenesis in primary lung cancer and its relation with lymph node metastasis, tumor cell apoptosis, and cell proliferation.


METHODS
Intratumor microvessel was detected by factor VIII polyclonal antibody using immunohistochemistry. Tumor cell apoptosis was detected by TUNEL method. Cell proliferation was analyzed by flow cytometry.


RESULTS
Intratumor microvessel density (MD) was significantly lower in stage I lung cancer than in stage II and stage III (P < 0.01). MD was significantly higher in the tumors with lymph node metastasis (P < 0.01). With the increase in MD, there would be a 1.2-fold increase in the risk of lymph node metastasis (P < 0.01). With the increase of MD, apoptosis index (AI) of the tumor significantly decreased, but no relationship between MD and cell proliferation index (PI) was observed.


CONCLUSIONS
Tumor angiogenesis promotes growth and metastasis of lung cancer. Tumor angiogenesis also can inhibit the apoptosis of lung cancer.
Shi
E
E
Department of Cardiac Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Jiang
X
X
Li
Y
Y
Xu
S
S
Yin
H
H
D000328:Adult; D000368:Aged; D017209:Apoptosis; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000098:blood supply* / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic