title: In vitro high frequency regeneration of plantlets of Vigna mungo and their ex vitro growth.
abstract: Of the five explants of V. mungo var. T9 used, the excised shoot tips gave best response with regard to offshoot formation followed by the embryonal axis explants. While a treatment comprising 0.5 mgL(-1) BAP, 0.5 mgL(-1) 2iP and 0.1 mgL(-1) NAA induced differentiation of an average 10 offshoots in shoot tip explants, only 3 offshoots were formed in the explants of embryonal axis in a treatment containing 0.5 mgL(-1) BAP and 0.1 mgL(-1) NAA, found optimum for them. Multiple shoots differentiated when explants with earlier regenerated and growing offshoots were first cultured in a treatment containing 0.1 mgL(-1) BAP, 0.25 mgL(-1) IAA and 5 mgL(-1) CCC and then subcultured in the same treatment but having only 1 mgL(-1) CCC. The isolated shoots rooted in 0.5 mgL(-1) IAA resulted in the formation of complete plantlets of an average height of 15 cm in 20 days. The in vitro-regenerated plants grew normally under field conditions and came to flowering as well.
authors.affiliation: Tissue Culture Laboratory, National Botanical Research Institute, Lucknow, India.
authors.forename: S
authors.initials: S
authors.lastname: Agnihotri
authors.forename: R R
authors.initials: RR
authors.lastname: Singh
authors.forename: H C
authors.initials: HC
authors.lastname: Chaturvedi
mesh_terms: D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D007887:Fabaceae / Q000502:physiology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D012038:Regeneration / Q000502:physiology*
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators