title: Extraction of natural vitamin E from wheat germ by supercritical carbon dioxide.
abstract: An efficient supercritical fluid extraction (SFE) process with carbon dioxide (SFE-CO(2)) was developed for the extraction of natural vitamin E (V(E)) from wheat germ. Both the pretreatment of extracted wheat germ and extraction conditions were optimized to ensure maximal V(E) yield. The extraction was undertaken at the extracting pressure of 4000-5000 psi, the extracting temperature of 40-45 degrees C, and the carbon dioxide flow rate of 2.0 mL/min for 90 min. An optimized pretreatment of wheat germ was usually necessary with a particle size of 30 mesh and a moisture content of 5.1%. A yield comparison of V(E) and its isomers extracted by supercritical CO(2) with those by conventional solvent extraction suggested that this SFE process was a practical process prospectively superior to conventional solvent extraction to prepare V(E) from wheat germ.
authors.affiliation: College of Food Science, China Agricultural University (CAU), 2 Yuanmingyuan Road-West, Beijing 100094, People's Republic of China. yiqiangge@hotmail.com
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Ge
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Bodi
authors.initials: B
authors.lastname: Hui
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
authors.forename: Shaoxiao
authors.initials: S
authors.lastname: Wang
authors.forename: Tongyi
authors.initials: T
authors.lastname: Cai
mesh_terms: D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D010316:Particle Size; D012997:Solvents; D014908:Triticum / Q000737:chemistry*; D014810:Vitamin E / Q000302:isolation & purification*
chemical_list: D012997:Solvents; D014810:Vitamin E; D002245:Carbon Dioxide