Quantitative analysis of estrogen receptor-alpha and -beta and exon 5 splicing variant mRNA in endometrial hyperplasia in perimenopausal women.
We used real-time reverse transcription-polymerase chain reaction (QRT-PCR) to detect wild-type ERalpha and ERbeta and their isoforms as well as exon 5 splicing variant mRNA ERalpha in various types of endometrial hyperplasia in perimenopausal women. Our data suggest that the exon 2 and 7 deletion ERalpha mRNA isoforms are present in endometrial tissue undergoing various hyperplastic states. Wild type ERbeta mRNA and their isoforms 2, 4, 5, 6 were observed in each type of assessed endometrial specimens, and ERalpha/delta5 was found only in hyperplasia complex group.
Witek
A
A
Department of Gynecological Endocrinology, Medical University of Silesia, Katowice, Poland. andwit@poczta.onet.pl
Mazurek
U
U
Paul
M
M
Bierzyńska-Macyszyn
G
G
Wilczok
T
T
D000328:Adult; D000368:Aged; D017398:Alternative Splicing / Q000235:genetics*; D002979:Climacteric; D004714:Endometrial Hyperplasia / Q000503:physiopathology*; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics*
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen