[A comparison of polymerase chain reaction with other two widely used methods in detection of Porphyromonas gingivalis].
OBJECTIVE
To compare the efficiency of polymerase chain reaction (PCR) with cultural method and indirect immunofluorescence (IF) in detection of Porphyromonas gingivalis (P.g) in oral plaque samples.


METHODS
92 subgingival plaque samples were collected from patients of three kinds of periodontal disease. A program using PCR to detect Fimbrilin gene (Fim A) was designed to detect P.g. The efficiency of this PCR method was compared with cultural method and IF.


RESULTS
The positive-rate of PCR was 94.6%, while that of cultural method and IF was only 48.9% and 68.5% respectively. The difference was significant in statistical analysis (P < 0.001).


CONCLUSIONS
PCR is more sensitive than culture method and IF in detecting pathogens of periodontal diseases. The study provides a simplified and efficient method for oral clinical use.
Jin
X
X
Beijing Stomatology Hospital, Capital University of Medical Science, Beijing 100050, China.
Yang
S
S
Wang
S
S
D000328:Adult; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*