title: Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts.
abstract: An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di- O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity- guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
authors.affiliation: Instituto de Química, UNAM, Ciudad Universitaria, Coyoacán, CP 04510, Mexico DF, Mexico.
authors.forename: Daniel Arrieta
authors.initials: DA
authors.lastname: Baez
authors.forename: Gerardo Zepeda
authors.initials: GZ
authors.lastname: Vallejo
authors.forename: Patricia Cano
authors.initials: PC
authors.lastname: Sánchez
authors.forename: Matilde Breña
authors.initials: MB
authors.lastname: Valle
authors.forename: Ricardo Reyes
authors.initials: RR
authors.lastname: Chilpa
authors.forename: Manuel Jiménez
authors.initials: MJ
authors.lastname: Estrada
mesh_terms: D005591:Chemical Fractionation; D003460:Crystallization; D004926:Escherichia coli / Q000235:genetics; D008745:Methylation; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D029846:Senna Plant; D012996:Solutions
chemical_list: D009153:Mutagens; D010936:Plant Extracts; D012996:Solutions; D012431:Rutin; D011794:Quercetin