Colony-blot assay with anti-p60 antibodies as a method for quick identification of Listeria in food.
The present study evaluated the ability to isolate Listeria from foods, using shortened procedure of sample enrichment followed by immunomagnetic separation or filtration methods, and serological identification of isolated bacteria by colony-blot and Western blot methods with anti-p60 antibodies. By these rapid methods, identification of Listeria was achieved in much shorter time (40-48 h) than with standard cultivation and biochemical identification procedures. The rapid methods used are easy to perform and, what is most important, their specificity is very high and fulfills the expectations. The possibility to select Listeria colonies growing on non-selective media by blotting with anti-p60 antiserum seems to be particularly valuable in examination of food samples containing/not too many Listeria (1-10 CFU/25 g). However, the blot method using anti-PepD mAb specific to unique region of L. monocytogenes p60 is necessary to distinguish L. monocytogenes from other Listeria species.
Wieckowska-Szakiel
Marzena
M
Department of Infectious Biology, Institute of Microbiology and Immunology, University of Lódź, Poland.
Bubert
Andreas
A
Rózalski
Marek
M
Krajewska
Urszula
U
Rudnicka
Wiesława
W
Rózalska
Barbara
B
D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D015169:Colony Count, Microbial; D005506:Food Contamination; D005516:Food Microbiology; D018189:Immunomagnetic Separation / Q000379:methods; D008087:Listeria / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013997:Time Factors
C078516:60 kDa protein, Listeria monocytogenes; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins