[Effects of selective preoperative intra-arterial infusion chemotherapy on apoptosis and proliferation of rectal cancer cells].
OBJECTIVE
To study the effects of anti-cancer drugs on apoptosis and proliferation of rectal cancer cells in vivo.


METHODS
114 patients with rectal cancer were treated by selective preoperative intra-arterial infusion chemotherapy. The rectal tumor tissues were derived before chemotherapy and 7 - 12 days after this treatment. In the tissues, apoptotic cells were examined by TUNEL method, and the expression of PCNA was examined by S-P staining.


RESULTS
Before chemotherapy, the mean AI of tumor cells was 5.81 per thousand before chemotherapy and 10.99 per thousand 7 - 12 days after chemotherapy (P < 0.01). The mean PI of tumor cells before chemotherapy and 7 - 12 days after chemotherapy was 49.27% and 57.38% (P < 0.01). The mean AI of the high PI group increased more evidently than that of the low PI group.


CONCLUSIONS
This treatment induced apoptosis of rectal cancer cells and inhibit tumor cell proliferation. The mean AI of the high PI group was higher than that of the low PI group. It is suggested that this operation should be performed shortly after chemotherapy.
Qi
X
X
Institute of General Surgery, Nanjing General Hospital, People's Liberation Army, Nanjing 210002, China.
Liu
F
F
Li
J
J
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D007261:Infusions, Intra-Arterial; D008297:Male; D008875:Middle Aged; D012004:Rectal Neoplasms / Q000188:drug therapy* / Q000473:pathology
D002945:Cisplatin; D005472:Fluorouracil