title: Synergistic action of beta-glucan and platelets on interleukin-8 production by human peripheral blood leukocytes.
abstract: The effects of human platelets on interleukin (IL)-8 production from human peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes (PMNs) stimulated with the fungal (1-->3)-beta-D-glucan schizophyllan (SPG) were examined using ELISA. PBMCs/PMNs in the presence of platelets and SPG enhanced IL-8 production in comparison with those in the presence of either platelets or SPG. IL-8 production was dependent on the concentration of platelets and incubation time, and the activity reached the maximal level at 18 h of incubation. These activities were also observed with the addition of platelets prestimulated with SPG to PBMCs. Addition of SPG directly enhanced expression of P-selectin on platelet membrane surfaces. These results suggest that platelets play a key role in the cytokine production of leukocytes induced by fungal (1-->3)-beta-D-glucans and might be mediated, at least in part, by P-selectin.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Suzuki
authors.forename: Aiko
authors.initials: A
authors.lastname: Tsuzuki
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ohshima
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Yadomae
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001792:Blood Platelets / Q000502:physiology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005936:Glucans / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000096:biosynthesis*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D019007:P-Selectin / Q000096:biosynthesis; D012566:Sizofiran / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013329:Structure-Activity Relationship
chemical_list: D000276:Adjuvants, Immunologic; D005936:Glucans; D016209:Interleukin-8; D019007:P-Selectin; D012566:Sizofiran