title: A proteome database of human primary T helper cells.
abstract: We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4+ human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [35S]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROTand NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.
authors.affiliation: Centre for Biotechnology, University of Turku and Abo Akademi University, Finland. tuula.nyman@btk.utu.fi
authors.forename: T A
authors.initials: TA
authors.lastname: Nyman
authors.forename: A
authors.initials: A
authors.lastname: Rosengren
authors.forename: S
authors.initials: S
authors.lastname: Syyrakki
authors.forename: T P
authors.initials: TP
authors.lastname: Pellinen
authors.forename: K
authors.initials: K
authors.lastname: Rautajoki
authors.forename: R
authors.initials: R
authors.lastname: Lahesmaa
mesh_terms: D030562:Databases, Protein; D006801:Humans; D020543:Proteome; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
chemical_list: D020543:Proteome