Tonin and kallikrein in the brain of transgenic rat line expressing human tissue kallikrein.
A transgenic rat line harboring the human tissue kallikrein gene was investigated for expression and activity of tonin and kallikrein in different regions of the brain. The introduction of the transgene into the rat genome produced a significant augmentation of the expression levels and activity of rat tissue kallikrein. The possibility that human kallikrein does not hydrolyze the rat substrate is probably responsible for the augmented expression of the rat enzyme. On the other hand, although expression of tonin was significantly reduced, tonin activity was not altered in most brain structures, except for cerebellum and neurohypophysis.
Lomez
Eliane S L
ES
Department of Physiology, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Araujo
Ronaldo C
RC
Bader
Michael
M
Pesquero
Joao B
JB
Pesquero
Jorge L
JL
D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007610:Kallikreins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020840:Tissue Kallikreins / Q000235:genetics* / Q000378:metabolism
D012313:RNA; D007610:Kallikreins; C117017:tonin; D020840:Tissue Kallikreins