Ds-like restless deletion derivatives occur in Tolypocladium inflatum and two foreign hosts, Neurospora crassa and Penicillium chrysogenum.
Single copies of the transposon Restless from Tolypocladium inflatum were introduced into Neurospora crassa and Penicillium chrysogenum. Excision of Restless from its donor site was investigated in N. crassa and in P. chrysogenum using direct selective conditions. In N. crassa, forward selection was also analyzed. Deleted Restless elements were frequently obtained in addition to the expected complete removal of Restless from its donor site. Similar deleted elements were also identified in T. inflatum employing a PCR amplification strategy. These deleted Restless copies strongly resemble maize Ds elements of various types, and direct repeated sequences of 3 to 16 bp were found to flank the truncated regions. In addition Ds1-like Restless elements were identified that carried foreign sequences between the inverted repeats. We discuss how Ds-like Restless elements might be generated by inaccurate excision from an active transposon copy.
Windhofer
Frank
F
Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Bochum, Germany.
Hauck
Katarzyna
K
Catcheside
David E A
DE
Kück
Ulrich
U
Kempken
Frank
F
D004251:DNA Transposable Elements / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D014644:Genetic Variation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D014162:Transfection; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004271:DNA, Fungal