Kinetic measurements using EPR imaging with a modulated field gradient.
EPR imaging with modulated field gradient was applied for the investigation of fast diffusion processes. Three different imaging methods are possible: spectral-temporal, spatio-temporal, and spectral-spatial imaging. The time resolution is on the order of seconds and the spatial resolution is in the micrometer region. The efficiency of this imaging technique is demonstrated for the penetration of the spin probe Tempol in the skin of hairless mice biopsies. The skin is normally protected against the penetration of water soluble substances by the horny layer, a resistive thin lipophilic layer. Overcoming this horny layer for water soluble ingredients is one of the main practical problems for the topical application of pharmaceutics which could be investigated by EPR imaging. Different images represent the penetration behavior of the water soluble Tempol in the skin after treatment with the penetration enhancer DMSO (Dimethylsulfoxide) and after removing the horny layer.
Herrling
Thomas
T
FOM Institute, University of Applied Science and Technology, TFH Berlin, Germany.
Fuchs
Jürgen
J
Groth
Norbert
N
D000818:Animals; D004058:Diffusion; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000378:metabolism*
D004121:Dimethyl Sulfoxide