A calcium-responsive transcription factor, CaRF, that regulates neuronal activity-dependent expression of BDNF.
Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, CaRF, that activates transcription from BDNF promoter III in a CaRE1-dependent manner. The transcriptional activity of CaRF is regulated in a calcium- and neuron-selective manner, suggesting that CaRF may confer selectivity upon the activity-dependent induction of BDNF exon III expression.
Tao
Xu
X
Division of Neuroscience, Children's Hospital, Boston, MA 02115, USA.
West
Anne E
AE
Chen
Wen G
WG
Corfas
Gabriel
G
Greenberg
Michael E
ME
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D004268:DNA-Binding Proteins; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter; D006801:Humans; D017403:In Situ Hybridization; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020218:Response Elements / Q000235:genetics*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D019208:Brain-Derived Neurotrophic Factor; C486724:CaRF protein, human; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010726:Phosphodiesterase Inhibitors; D014157:Transcription Factors; D000242:Cyclic AMP; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine