Quantitation of DNA sequences in environmental PCR products by a multiplexed, bead-based method.
A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.
Spiro
Alexander
A
Physics Department, Loyola College in Maryland, 4501 N. Charles Street, Baltimore, MD 21210, USA.
Lowe
Mary
M
D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D005434:Flow Cytometry; D005618:Fresh Water / Q000382:microbiology*; D008863:Microspheres; D016133:Polymerase Chain Reaction; D011137:Polystyrenes; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014877:Water Pollution, Chemical
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011137:Polystyrenes; D012336:RNA, Ribosomal, 16S