Analysis of artichoke (Cynara cardunculus L.) extract by means of micellar electrokinetic capillary chromatography.
The main constituents of artichoke extract were separated by micellar electrokinetic chromatography (MEKC), using a buffer consisting of 100 mM sodium dodecyl sulfate (SDS) in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate (pH 8.6) as background electrolyte. Optimum separation voltage of 28 kV (positive polarity) and a capillary temperature of 25 degrees C gave the best analysis. The UV detection was performed at 200 nm. The method was successfully used to analyze plant and drug samples as well as for the study of artichoke antioxidant activity. The quantitative MEKC results were in good agreement to those obtained previously by reversed-phase high-performance liquid chromatography (RP-HPLC).
Sevcíková
Petra
P
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Glatz
Zdenek
Z
Slanina
Jirí
J
D019659:Asteraceae / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry
D010936:Plant Extracts