Cloning and sequence analysis of the complementary DNA for feline preproparathyroid hormone.
OBJECTIVE
To clone and sequence the cDNA for feline preproparathyroid hormone (preproPTH) and to compare that sequence with other known parathyroid hormone (PTH) sequences.


METHODS
Parathyroid glands from 1 healthy cat.


METHODS
A cDNA library was constructed in lambda phage from feline parathyroid gland mRNA and screened with a radiolabeled canine PTH probe. Positive clones were sequenced, and nucleic acid and deduced amino acid sequences were analyzed and compared with known preproPTH and PTH sequences.


RESULTS
Screening of approximately 2 X 10(5) recombinant plaques revealed 3 that hybridized with the canine PTH probe; 2 clones comprised the complete sequence for feline preproPTH. Feline preproPTH cDNA consisted of a 63-base pair (bp) 5'-untranslated region (UTR), a 348-bp coding region, and a 326-bp 3'-UTR. The coding region encoded a 115-amino acid peptide. Mature feline PTH consisted of 84 amino acids. Amino acid sequence analysis revealed that feline PTH was > 83% identical to canine, bovine, swine, equine, human, and macaque PTH and 69, 71, and 44% identical to mouse, rat, and chicken PTH, respectively. Within the region responsible for hormonal activity (amino acids 1 to 34), feline PTH was > 79% identical to other mammalian PTH sequences and 64% identical to the chicken sequence.


CONCLUSIONS
The amino acid sequence of PTH is conserved among mammalian species. Knowledge of the cDNA sequence for feline PTH may be useful to investigate disturbances of calcium metabolism and alterations in PTH expression in cats.
Toribio
Ramiro E
RE
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus 43210, USA.
Kohn
Catherine W
CW
Chew
Dennis J
DJ
Capen
Charles C
CC
Rosol
Thomas J
TJ
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D010582:Bacteriophage lambda; D001483:Base Sequence; D002415:Cats / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D006736:Horses; D006801:Humans; D008251:Macaca; D008969:Molecular Sequence Data; D010280:Parathyroid Glands / Q000737:chemistry; D010281:Parathyroid Hormone / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D013552:Swine
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D010281:Parathyroid Hormone; D011498:Protein Precursors; D012333:RNA, Messenger; C019056:preproparathormone