Modulation of c-myc and c-fos gene expression in regenerating rat liver by 2-mercaptopropionylglycine.
Expression of the two proto-oncogenes, c- myc and c- fos, in proliferating liver, and the modulation of their expression by 2-mercaptopropionylglycine (MPG) has been investigated. A significant increase in the expression of both c-myc and c-fos was observed, attaining a peak at 30 min, followed by a gradual decline for 8h after partial hepatectomy. Treatment of partially hepatectomized animals with MPG resulted in a significant decrease in the expression of both genes, the decrease being more marked in c- myc mRNA levels. At later time points, there was a very small rise in the mRNA levels of these genes in MPG-treated animals but they still remained much lower than the peak levels observed in both genes 30 min after surgery. A decline in the recovery of liver weight in MPG-treated animals was also observed.
Ohri
Sujata
S
Gene Regulation Laboratory, Centre for Biotechnology, Jawaharlal Nehru University, New Delhi, India.
Sharma
Dipali
D
Dixit
Aparna
A
D000818:Animals; D002455:Cell Division; D015536:Down-Regulation; D005786:Gene Expression Regulation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D009929:Organ Size; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012038:Regeneration; D013997:Time Factors; D008625:Tiopronin / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D008625:Tiopronin