Recent advances in DNA sequencing by capillary and microdevice electrophoresis.
A number of significant improvements in the electrophoretic performance and design of DNA sequencing devices have culminated in the introduction of truly industrial grade production scale instruments. These instruments have been the workhorses behind the massive increase in genomic sequencing data available in public and private databases. We highlight the recent progress in aspects of capillary electrophoresis (CE) that has enabled these achievements. In addition, we summarize recent developments in the use of microfabricated devices for DNA sequencing that promise to bring the next leap in productivity.
Mitnik
L
L
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. lmitnik-gankin@wi.mit.edu
Novotny
M
M
Felten
C
C
Buonocore
S
S
Koutny
L
L
Schmalzing
D
D
D019075:Electrophoresis, Capillary / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*