title: Longitudinal analysis of feline leukemia virus-specific cytotoxic T lymphocytes: correlation with recovery from infection.
abstract: Feline leukemia virus (FeLV) is a common naturally occurring gammaretrovirus of domestic cats that is associated with degenerative diseases of the hematopoietic system, immunodeficiency, and neoplasia. Although the majority of cats exposed to FeLV develop a transient infection and recover, a proportion of cats become persistently viremic and many subsequently develop fatal diseases. To define the dominant host immune effector mechanisms responsible for the outcome of infection, we studied the longitudinal changes in FeLV-specific cytotoxic T lymphocytes (CTLs) in a group of naïve cats following oronasal exposure to FeLV. Using (51)Cr release assays to measure ex vivo virus-specific cytotoxicity, the emerging virus-specific CTL response was correlated with modulations in viral burden as assessed by detection of infectious virus, FeLV p27 capsid antigen, and proviral DNA in the blood. High levels of circulating FeLV-specific effector CTLs appeared before virus neutralizing antibodies in cats that recovered from exposure to FeLV. In contrast, persistent viremia was associated with a silencing of virus-specific humoral and cell-mediated host immune effector mechanisms. A single transfer of between 2 x 10(7) and 1 x 10(8) autologous, antigen-activated lymphoblasts was associated with a downmodulation in viral burden in vivo. The results suggest an important role for FeLV-specific CTLs in retroviral immunity and demonstrate the potential to modulate disease outcome by the adoptive transfer of antigen-specific T cells in vivo.
authors.affiliation: Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden, Glasgow G61 1QH, Scotland. n.flynn@vet.gla.ac.uk
authors.forename: J Norman
authors.initials: JN
authors.lastname: Flynn
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Dunham
authors.forename: Vivien
authors.initials: V
authors.lastname: Watson
authors.forename: Oswald
authors.initials: O
authors.lastname: Jarrett
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002415:Cats; D004279:DNA, Viral / Q000097:blood; D015683:Gene Products, gag / Q000097:blood; D005256:Leukemia Virus, Feline / Q000276:immunology* / Q000302:isolation & purification; D011533:Proviruses; D012192:Retroviridae Infections / Q000276:immunology* / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014412:Tumor Virus Infections / Q000276:immunology* / Q000821:virology; D014766:Viremia / Q000276:immunology* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015683:Gene Products, gag; C074662:p24 protein, Feline immunodeficiency virus