Dietary adrenergic active compounds and the response of broilers to isoproterenol and cyclic adenosine monophosphate in vitro.
Broiler chickens, growing from 7-28 days of age, were fed diets containing 18% protein and 0, 1, 10 or 100 mg/kg yohimbine (alpha 2-adrenergic antagonist) or metaproterenol (beta-adrenergic agonist) to determine the role of adrenergic agents in the regulation of feeding behavior and metabolism. Data from this experiment suggest that beta-adrenergic agonists have slight effects on feed intake, growth and more pronounced effects on metabolism in the broiler chicken. In vitro lipogenesis (IVL) was determined by incubating liver explants for 2 h at 37 degrees C in the presence of cAMP or isoproterenol (ISO) and [2-14C]acetate and by measuring acetate incorporation into total hepatic lipid. Metaproterenol and yohimbine (100 mg/kg) depressed growth from 7 to 28 days. Both metaproterenol and yohimbine (100 mg/kg) decreased (P < 0.05) IVL compared to controls. These dietary additions also decreased (P < 0.05) hepatic malic enzyme activity without affecting the activities of either isocitrate dehydrogenase or aspartate aminotransferase.
Rosebrough
R W
RW
Growth Biology Laboratory, Livestock and Poultry Science Institute, Dortmund United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA. Rosebro@ANRI.BARC.USDA.GOV
D000317:Adrenergic alpha-Antagonists / Q000008:administration & dosage* / Q000494:pharmacology*; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage* / Q000494:pharmacology*; D000818:Animals; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009921:Metaproterenol / Q000008:administration & dosage* / Q000494:pharmacology*; D015016:Yohimbine / Q000008:administration & dosage* / Q000494:pharmacology*
D000317:Adrenergic alpha-Antagonists; D000318:Adrenergic beta-Agonists; D015016:Yohimbine; D009921:Metaproterenol; D000242:Cyclic AMP; D007545:Isoproterenol