title: Integrated gene and species phylogenies from unaligned whole genome protein sequences.
abstract: BACKGROUND
  Most molecular phylogenies are based on sequence alignments. Consequently, they fail to account for modes of sequence evolution that involve frequent insertions or deletions. Here we present a method for generating accurate gene and species phylogenies from whole genome sequence that makes use of short character string matches not placed within explicit alignments. In this work, the singular value decomposition of a sparse tetrapeptide frequency matrix is used to represent the proteins of organisms uniquely and precisely as vectors in a high-dimensional space. Vectors of this kind can be used to calculate pairwise distance values based on the angle separating the vectors, and the resulting distance values can be used to generate phylogenetic trees. Protein trees so derived can be examined directly for homologous sequences. Alternatively, vectors defining each of the proteins within an organism can be summed to provide a vector representation of the organism, which is then used to generate species trees.
  
  
  RESULTS
  Using a large mitochondrial genome dataset, we have produced species trees that are largely in agreement with previously published trees based on the analysis of identical datasets using different methods. These trees also agree well with currently accepted phylogenetic theory. In principle, our method could be used to compare much larger bacterial or nuclear genomes in full molecular detail, ultimately allowing accurate gene and species relationships to be derived from a comprehensive comparison of complete genomes. In contrast to phylogenetic methods based on alignments, sequences that evolve by relative insertion or deletion would tend to remain recognizably similar.
authors.affiliation: Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, USA. G-Stuart@indstate.edu
authors.forename: Gary W
authors.initials: GW
authors.lastname: Stuart
authors.forename: Karen
authors.initials: K
authors.lastname: Moffett
authors.forename: Steve
authors.initials: S
authors.lastname: Baker
mesh_terms: D000465:Algorithms; D000818:Animals; D019295:Computational Biology; D030562:Databases, Protein; D016678:Genome; D008322:Mammals / Q000145:classification / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment / Q000706:statistics & numerical data; D012984:Software; D013045:Species Specificity
chemical_list: D024101:Mitochondrial Proteins; D011506:Proteins