title: Aberrant expression of complement regulatory proteins, membrane cofactor protein and decay accelerating factor, in the involved epidermis of patients with vitiligo.
abstract: BACKGROUND
  Vitiligo is a pigmentary disorder of the skin characterized by the complete absence of melanocytes from the lesion. Complement-activating antimelanocyte antibodies have been implicated in vitiligo pathogenesis. As membrane regulators of complement activation, membrane cofactor protein, decay accelerating factor and CD59 protect cells from elimination by autologous complement, their absence or downregulation on melanocytes may be associated with autoantibody and complement-mediated melanocyte destruction in vitiligo.
  
  
  OBJECTIVE
  We studied the expression of these regulatory proteins in non-lesional, perilesional and lesional vitiligo skin compared with those of control specimens.
  
  
  METHODS
  We used immunohistochemistry to study the expression of the regulatory proteins, and flow cytometric analysis of cultured melanocytes to investigate possible constitutive changes in the expression levels of these molecules. We also investigated whether melanocytes can influence keratinocyte susceptibility to autologous complement by regulating keratinocytic decay accelerating factor and membrane cofactor protein expression levels.
  
  
  RESULTS
  Immunohistochemical data showed that expression of membrane cofactor protein and decay accelerating factor in whole epidermis was lower in lesional and perilesional skin in comparison with non-lesional skin. The reduced in situ expression appeared to be specific to vitiligo. However, coculture experiments indicated that melanocytes do not influence keratinocyte susceptibility to autologous complement. Further, flow cytometric analysis of cultured melanocytes convincingly demonstrated that non-lesional vitiligo and control melanocytes have comparable decay accelerating factor, membrane cofactor protein and CD59 expression levels.
  
  
  CONCLUSIONS
  It is therefore concluded that there is no constitutive melanocyte defect per se that could be related to the in vivo expression of these molecules in vitiligo. Nevertheless, the present data suggest that both keratinocytes and melanocytes in the involved vitiliginous whole epidermis express lower levels of decay accelerating factor and membrane cofactor protein compared with controls that could render them more vulnerable to autologous complement attack.
authors.affiliation: Department of Pathology (Room L-2-258), Academic Medical Centre, Amsterdam University, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
authors.forename: R M J G J
authors.initials: RM
authors.lastname: van den Wijngaard
authors.forename: S S
authors.initials: SS
authors.lastname: Asghar
authors.forename: A C L M
authors.initials: AC
authors.lastname: Pijnenborg
authors.forename: A J
authors.initials: AJ
authors.lastname: Tigges
authors.forename: W
authors.initials: W
authors.lastname: Westerhof
authors.forename: P K
authors.initials: PK
authors.lastname: Das
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D018958:CD55 Antigens / Q000378:metabolism*; D018957:CD59 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D004817:Epidermis / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008544:Melanocytes / Q000378:metabolism; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000378:metabolism*; D014820:Vitiligo / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C497857:CD46 protein, human; D018958:CD55 Antigens; D018957:CD59 Antigens; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins