Evaluation of the energetic contribution of an ionic network to beta-sheet stability.
We have evaluated the interaction energy of a three-residue ionic network constructed on the beta-sheet surface of protein G using double mutant cycles. Although the two individual ion pairs were each stabilizing by 0.6 kcal/mol, the excess gain in stability for the triad was small (0.06 kcal/mol).
Lassila
Kirsten S
KS
Biochemistry Option, California Institute of Technology, MC 147-75, Pasadena, California 91125, USA.
Datta
Deepshikha
D
Mayo
Stephen L
SL
D000596:Amino Acids / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D013816:Thermodynamics
D000596:Amino Acids; C029152:G-substrate; D007477:Ions; D009419:Nerve Tissue Proteins