title: [The growth inhibitory effects by transfection of p16 gene on human pancreatic cancer cell line].
abstract: OBJECTIVE
  To elevate the growth inhibitory effects by transfection of p16 gene on human pancreatic cancer cell line JF305.
  
  
  METHODS
  Recombinant eukaryotic expression vector pDOR-p16 containing exogenous human wt-p16 cDNA and vector containing neomycin resistance gene only were introduced by Liposomes-mediated gene transfection into JF305 cell line which did not express endogenous p16. By using PCR amplification, in situ hybridization, and immunocytochemistry, the clones obtained were detected for efficiency of transfection and effect of vector expression and observed for the changes of their biologic characteristics.
  
  
  RESULTS
  Exogenous wt-p16 was successfully transferred into JF305 cells and obtained permanent expression. The growth rate of these transfected JF305 cells in regular medium and soft agar was inhibited. The percentage of phage G(1) cells increased and that of phage S cells decreased by analysing cell cycle. The ultrastructural changes of the cells observed under electron-microscope revealed growth retardation.
  
  
  CONCLUSIONS
  p16 is a candidate for cancer gene replacement therapy of human pancreatic cancer.
authors.affiliation: Department of Surgery, First Clinical College, China Medical University, Shenyang 110001, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Zhang
mesh_terms: D002455:Cell Division; D045744:Cell Line, Tumor; D019942:Genes, p16; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection