title: Entamoeba histolytica: transferrin binding proteins.
abstract: Entamoeba histolytica trophozoites depend on iron for their growth; thus, they must use some host iron-containing molecules to fulfill this requirement. In this work we report that amoebas are able to utilize human holo-Tf as iron source and to recognize it through transferrin binding proteins. By use of an anti-human transferrin antiserum in an immunoblotting assay, two main polypeptides with apparent molecular masses of 70 and 140 kDa were found in total extract of trophozoites cultured in vitro. However, when a monoclonal anti-human transferrin receptor antibody was used, only one band with molecular mass of 140 kDa was observed. Both the human transferrin and the monoclonal antibody recognized a protein on the amoebic surface, demonstrated by confocal microscopy. Furthermore, the complex transferrin-transferrin binding protein was internalized by an endocytic process and probably dissociated inside the cell. This mechanism could be one manner in which E. histolytica acquires iron from the human host transferrin.
authors.affiliation: Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del I.P.N., México, D.F., México.
authors.forename: M
authors.initials: M
authors.lastname: Reyes-López
authors.forename: J J
authors.initials: JJ
authors.lastname: Serrano-Luna
authors.forename: E
authors.initials: E
authors.lastname: Negrete-Abascal
authors.forename: N
authors.initials: N
authors.lastname: León-Sicairos
authors.forename: A L
authors.initials: AL
authors.lastname: Guerrero-Barrera
authors.forename: M
authors.initials: M
authors.lastname: de la Garza
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004705:Endocytosis; D004748:Entamoeba histolytica / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D033862:Iron-Binding Proteins; D018613:Microscopy, Confocal; D015800:Protozoan Proteins / Q000032:analysis* / Q000378:metabolism; D011990:Receptors, Transferrin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*; D033863:Transferrin-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D007106:Immune Sera; D033862:Iron-Binding Proteins; D015800:Protozoan Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D033863:Transferrin-Binding Proteins