title: Genomic structure of the human beta-PIX gene and its alteration in gastric cancer.
abstract: beta-PIX, a newly identified p21-activated kinase (PAK)-interacting exchange factors (PIX), encodes a guanine nucleotide exchange factor for Rho guanosine triphosphatases. Characterization of beta-PIX gene was performed using the BAC Library method. The beta-PIX gene has 17 exons and an A/T polymorphism at the 32nd base upstream of the intron/exon junction of exon 7. The frequencies of genotypes A/T, A/A and T/T were 23.6% (13/55), 72.7% (40/55) and 3.6% (2/55), respectively; these frequencies are in Hardy-Weinberg equilibrium. Two out of 14 informative tumors (14.3%) were shown to have lost their heterozygosity at this locus, but no mutations in the remaining alleles were detected. In addition, we examined the gene-expression profile in another set of 30 gastric samples, but no significant over-expression of either the beta-PIX gene or the alpha-PIX gene was found. Though the beta-PIX gene has been speculated to potentially have tumor-related biological characteristics, the findings of the present study suggest that the involvement of beta-PIX gene in human gastric carcinogenesis is minimal.
authors.affiliation: First Department of Pathology, Hamamatsu University School of Medicine, 1-20-1, Handayama, Hamamatsu 431-3192, Japan.
authors.forename: Zhong you
authors.initials: Zy
authors.lastname: Li
authors.forename: You jie
authors.initials: Yj
authors.lastname: Wang
authors.forename: Jian ping
authors.initials: Jp
authors.lastname: Song
authors.forename: Hideki
authors.initials: H
authors.lastname: Kataoka
authors.forename: Shigeto
authors.initials: S
authors.lastname: Yoshii
authors.forename: Chang ming
authors.initials: Cm
authors.lastname: Gao
authors.forename: Ya ping
authors.initials: Yp
authors.lastname: Wang
authors.forename: Jian nong
authors.initials: Jn
authors.lastname: Zhou
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ota
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
mesh_terms: D000483:Alleles; D018797:Cell Cycle Proteins / Q000235:genetics*; D015894:Genome, Human; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D011110:Polymorphism, Genetic; D064067:Rho Guanine Nucleotide Exchange Factors; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*
chemical_list: C576606:ARHGEF7 protein, human; D018797:Cell Cycle Proteins; D020662:Guanine Nucleotide Exchange Factors; D064067:Rho Guanine Nucleotide Exchange Factors