[Plasminogen activator inhibitor-1 mRNA expression by the iris and lens epithelial cells of rabbit eye with intraocular lens implantation].
OBJECTIVE
To investigate the change of plasminogen activator inhibitor-1 (PAI-1) synthesized by the iris and the lens epithelial cells (LECs) after extracapsular cataract extraction (ECCE) with intraocular lens (IOL) implantation and the role of PAI-1 in the occurrence of fibrin reaction and the formation of posterior capsular opacification.


METHODS
Eight adult rabbits were studied. In each rabbit, one eye underwent ECCE with IOL implantation and the other eye was normal without any surgical intervention. The iris tissue and the lens capsule of each two normal eyes and each two surgical eyes were excised on the postoperative 1, 7, 15 and 30 days respectively. The amount of PAI-1 mRNA expression in the specimens was detected by using reverse transcription-polymerase chain reaction (RT-PCR). The data were analyzed statistically.


RESULTS
The amount of PAI-1 mRNA expresson in the iris tissue and the LECs of the surgical eyes displayed a significant increase on the first day after operation. The high expression of PAI-1 mRNA by the iris might last 15 days (P < 0.001), but it declined to the normal range on the postoperative 30 days (P = 0.87). The high level of PAI-1 mRNA expresson by the LECs in the surgical eyes had significant difference when compared with that of the normal eyes at 1, 7, 15 and 30 days postoperatively (P < 0.001).


CONCLUSIONS
It is indicated that PAI-1 enzyme is a major cause for fibrin reaction after ECCE with IOL implantation, and may play critical roles in the development of posterior capsular opacification.
Wu
Q
Q
Department of Ophthalmology, Shanghai No.6 Hospital, Shanghai 200233.
Yang
G
G
Chen
G
G
Dai
Y
Y
D000818:Animals; D002387:Cataract Extraction; D004847:Epithelial Cells / Q000378:metabolism*; D007498:Iris / Q000378:metabolism*; D019654:Lens Implantation, Intraocular; D007908:Lens, Crystalline / Q000166:cytology*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger