Steps toward mapping the human vasculature by phage display.
The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47,160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand-receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.
Arap
Wadih
W
Department of Genito-Urinary Medical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA. warap@notes.mdacc.tmc.edu
Kolonin
Mikhail G
MG
Trepel
Martin
M
Lahdenranta
Johanna
J
Cardó-Vila
Marina
M
Giordano
Ricardo J
RJ
Mintz
Paul J
PJ
Ardelt
Peter U
PU
Yao
Virginia J
VJ
Vidal
Claudia I
CI
Chen
Limor
L
Flamm
Anne
A
Valtanen
Heli
H
Weavind
Lisa M
LM
Hicks
Marshall E
ME
Pollock
Raphael E
RE
Botz
Gregory H
GH
Bucana
Corazon D
CD
Koivunen
Erkki
E
Cahill
Dolores
D
Troncoso
Patricia
P
Baggerly
Keith A
KA
Pentz
Rebecca D
RD
Do
Kim-Anh
KA
Logothetis
Christopher J
CJ
Pasqualini
Renata
R
D001808:Blood Vessels / Q000502:physiology*; D014644:Genetic Variation; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry; D009928:Organ Specificity; D019151:Peptide Library; D015203:Reproducibility of Results; D012984:Software
D009842:Oligopeptides; D019151:Peptide Library