Deformin, a substance found in Bartonella bacilliformis culture supernatants, is a small, hydrophobic molecule with an affinity for albumin.
Culture supernatants of Bartonella bacilliformis were previously shown to contain a factor, called deforming factor or deformin, which causes deformation and invagination of red cell membranes and formation of intracellular vacuoles. This factor is here shown to be a small water-soluble molecule, approximately 1400 Da as estimated by gel-filtration chromatography. Deforming factor binds tightly to albumin, especially albumin dimers and multimers, present in the growth medium. It can be released from albumin with 50% ethanol and has been partially purified by filtration and HPLC.
Derrick
S C
SC
Laboratory of Mycobacteria, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA.
Ihler
G M
GM
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism; D001473:Bartonella / Q000737:chemistry*; D002845:Chromatography; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D019281:Dimerization; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D004913:Erythrocytes, Abnormal / Q000382:microbiology / Q000473:pathology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D012709:Serum Albumin; C092807:deformin protein, Bartonella bacilliformis