title: Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography was applied to the isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude salvianolic acid B was obtained by extraction with ethanol-water from S. miltiorrhiza Bunge. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (3:7:1:9, v/v) was successfully performed yielding 342 mg salvianolic acid B at 98% purity from 500 mg of the crude extract in a one-step separation.
authors.affiliation: Department of Botany, The University of Hong Kong, China.
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Li
authors.forename: Jia-Ping
authors.initials: JP
authors.lastname: Lai
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D027544:Salvia / Q000737:chemistry*; D053630:Depsides
chemical_list: D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides