Granulocyte-colony stimulating factor enhances the expression of transforming growth factor-beta mRNA in CD4-positive peripheral blood lymphocytes in the donors for allogeneic peripheral blood stem cell transplantation.
The degree of acute graft-versus-host disease (GVHD) after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) has been observed to be, unexpectedly, of an equal level to that after bone marrow transplantation. To explain this phenomenon, we hypothesized that granulocyte-colony stimulating factor (G-CSF) administration may induce transforming growth factor (TGF)-beta producing T cells in the donors. Five donors received 10 microg/kg G-CSF subcutaneously for 4 days. The TGFbeta mRNA expression in CD4(+) cells as measured by real time reverse transcription-polymerase chain reaction increased after G-CSF administration. This elevation is considered to be one additive mechanism of repression of acute GVHD after allo-PBSCT.
Hirayama
Yasuo
Y
Fourth Department Internal Medicine, Sapporo Medical University, Sapporo, Japan. hira950712@aol.com
Sakamaki
Sumio
S
Matsunaga
Takuya
T
Kuroda
Hiroyuki
H
Kusakabe
Toshiro
T
Akiyama
Takehide
T
Kato
Junji
J
Kogawa
Katsuhisa
K
Koyama
Ryuzo
R
Nagai
Tadanori
T
Ohta
Hidetoshi
H
Niitsu
Yoshiro
Y
D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D006086:Graft vs Host Disease / Q000517:prevention & control; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D018655:Lymphocyte Count; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018417:Th1 Cells; D018418:Th2 Cells; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014184:Transplantation, Homologous
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016179:Granulocyte Colony-Stimulating Factor