Functional dissection of a Rice Dr1/DrAp1 transcriptional repression complex.
We characterized rice cDNA sequences for OsDr1 and OsDrAp1, which encode structural homologs of the eukaryotic general repressors Dr1 and DrAp1, respectively. OsDr1 and OsDrAp1 are nuclear proteins that interact with each other and with the TATA binding protein/DNA complex. In vitro and in vivo functional analyses showed that OsDrAp1 functions as a repressor, unlike its role in other eukaryotic systems, in which DrAp1 is a corepressor. OsDr1 and OsDrAp1 functioned together as a much stronger repressor than either one alone. Functional dissections revealed that the N-terminal histone-fold domains of OsDr1 and OsDrAp1 were necessary and sufficient for their repression and protein-protein interaction with each other. The unique glutamine- and proline-rich domain of OsDr1 had no repression activity. The basic amino acid-rich region and an arginine and glycine repeat domain of OsDrAp1 enhanced its repression activity. Thus, although OsDr1 and OsDrAp1 function as repressors, the functions of the two components are reversed compared with those of their nonplant counterparts.
Song
Wen
W
Central Research and Development, DuPont Company, P.O. Box 80402, Wilmington, DE 19880-0402, USA.
Solimeo
Harry
H
Rupert
Ross A
RA
Yadav
Narendra S
NS
Zhu
Qun
Q
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012097:Repressor Proteins / Q000235:genetics*; D016385:TATA Box / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection / Q000379:methods
D018076:DNA, Complementary; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; C075995:down-regulator of transcription 1