title: O-serogroups, eae gene and EAF plasmid in Escherichia coli isolates from cases of bovine mastitis in Brazil.
abstract: Mastitis has been recognized for some time as the most costly disease in dairy herds. From March 1997 to August 1998, 2144 samples of bovine mastitic milk were collected, from which 182 Escherichia coli isolates were made, and from which 141 isolates had the somatic antigen (serogroup) determined. Twelve different serogroups were isolated from mastitic milk, and among them were O26, O55, O111 and O119, all of them classic enteropathogenic E. coli (EPEC) serogroups. These represented 40.0% of the isolates. The 20 of 57 isolates tested had plasmids and in dot blot hybridization, nine isolates were positive for an EaeA probe and an EPEC adherence factor (EAF) probe while two isolates were negative for EaeA probe but positive for the EAF probe. The nine isolates were characterized as attaching and effacing (A/E) E. coli (AEEC) isolates.
authors.affiliation: Departamento de Microbiologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, SP, Ribeirao Preto, Brazil.
authors.forename: M G P
authors.initials: MG
authors.lastname: Correa
authors.forename: J M
authors.initials: JM
authors.lastname: Marin
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D001938:Brazil; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D015342:DNA Probes; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005260:Female; D015151:Immunoblotting; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D019081:O Antigens / Q000032:analysis / Q000276:immunology; D010957:Plasmids; D012703:Serotyping
chemical_list: D018829:Adhesins, Bacterial; D002352:Carrier Proteins; D015342:DNA Probes; D029968:Escherichia coli Proteins; D019081:O Antigens; C073819:eaeA protein, E coli