title: Use of pulsed-field gel electrophoresis, enterobacterial repetitive intergenic consensus typing, and automated ribotyping to assess genomic variability among strains of nontypeable Haemophilus influenzae.
abstract: We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques as the sole method to group NT H. influenzae strains.
authors.affiliation: Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109-2029, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Pettigrew
authors.forename: B
authors.initials: B
authors.lastname: Foxman
authors.forename: Z
authors.initials: Z
authors.lastname: Ecevit
authors.forename: C F
authors.initials: CF
authors.lastname: Marrs
authors.forename: J
authors.initials: J
authors.lastname: Gilsdorf
mesh_terms: D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D016680:Genome, Bacterial; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D021521:Ribotyping; D012371:Robotics