Accumulation of osmoprotectants and lipid pattern modulation in response to growth conditions by Halomonas pantelleriense.
The effects of salinity, growth temperature, pH and composition of the medium on the accumulation of intracellular organic solutes, by nuclear magnetic resonance spectroscopy (NMR) in Halomonas pantelleriense were examined. The modulation of lipid pattern in different growth conditions was also reported. H. pantelleriense accumulated glycine betaine, ectoine, hydroxyectoine and glutamate. The type of osmoprotectant and the relative proportion depended on growth conditions. The main lipids identified by NMR studies were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), 1,2 diacylglycero-3-phosphoryl-glycerol (PG) and cardiolipin, (DPG). The predominant fatty acids were C16:0 and C18:1, minor fatty acids were C16:1 and C18:0. The relative percentage of polar lipids and fatty acids were affected by growth conditions.
Romano
I
I
Istituto per la Chimica di Molecole di Interesse Biologico, CNR, Comprensorio ex-Olivetti Fabbricato 70, Pozzuoli, Na, Italy.
Nicolaus
B
B
Lama
L
L
Trabasso
D
D
Caracciolo
G
G
Gambacorta
A
A
D020632:Halomonas / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D014882:Water-Electrolyte Balance
D008055:Lipids; D012965:Sodium Chloride