title: Viability of fibroblasts in cell culture after treatment with different chemical retraction agents.
abstract: Prior to fixed prosthodontic impression procedures, temporary horizontal retraction of the free gingival tissue should be accomplished apically to the preparation finishing line. The mechanical-chemical method using cotton retraction cords of various sizes impregnated with various retraction chemicals is the most commonly employed retraction technique. Most retraction agents have pH values from 0.8 to 0.3, and are therefore hazardous to the cut dentine and periodontal tissues. Sympathomimetic vasoconstrictors introduced recently have a pH of 5.6, and are free of systemic side-effects. The present study using the dye exclusion test, colony forming ability test and colorimetric assay was undertaken to evaluate cytotoxic effects of four chemical retraction agents on cultured V-79 fibroblasts, and the dependence of cytotoxicity on the agent concentration and time of exposure. Original concentrations of retraction agents produced stronger cytotoxic effects than dilutions of 1:1 and 1:10. The most aggressive agent, 25% aluminium chloride, took only 1 min to damage all cell cultures. The proportion of cells damaged after 10 min of exposure to tetrahydrozoline was 60%, which was significantly less compared with other chemicals tested. With the colony forming ability test using retraction agents diluted to 1:10 the greatest number of colonies emerged in samples treated with tetrahydrozoline (statistical significance: P < 0.01). The colorimetric assay showed equal cytotoxic effects for 25% aluminium sulphate and tetrahydrozoline. The colorimetric test used in the study has proved an ergonomic, accurate and reliable test for cytotoxicity determination.
authors.affiliation: Center of Fixed Prosthodontics and Occlusion, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia. igor.kopac@mf.uni-lj.si
authors.forename: I
authors.initials: I
authors.lastname: Kopac
authors.forename: U
authors.initials: U
authors.lastname: Batista
authors.forename: E
authors.initials: E
authors.lastname: Cvetko
authors.forename: L
authors.initials: L
authors.lastname: Marion
mesh_terms: D000534:Alum Compounds / Q000008:administration & dosage / Q000633:toxicity; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D001252:Astringents / Q000008:administration & dosage / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000008:administration & dosage / Q000633:toxicity; D003124:Colorimetry; D004396:Coloring Agents; D006224:Cricetinae; D003761:Dental Impression Technique; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000008:administration & dosage / Q000633:toxicity; D015203:Reproducibility of Results; D013566:Sympathomimetics / Q000008:administration & dosage / Q000633:toxicity; D013997:Time Factors; D014343:Trypan Blue
chemical_list: D000534:Alum Compounds; D017607:Aluminum Compounds; D001252:Astringents; D002712:Chlorides; D004396:Coloring Agents; D007093:Imidazoles; D013566:Sympathomimetics; C041524:aluminum sulfate; D000077410:Aluminum Chloride; D014343:Trypan Blue; C005810:tetrahydrozoline