Intestinal absorption and lymphatic transport of a high gamma-linolenic acid canola oil in lymph fistula Sprague-Dawley rats.
A new canola strain capable of producing >30% gamma-linolenic acid [GLA, 18:3(n-6)] in the seed oil has been developed in our laboratories. This study compares the intestinal absorption and lymphatic transport of this newly developed high GLA content canola oil (HGCO) with traditional GLA-rich borage oil (BO) using a lymph fistula rat model. To assess the extent that 1 mL of GLA in the supplemented oil was absorbed and transported, the fatty acid compositions of triglycerides in mesenteric lymph were compared over a 24-h collection period. The digestion, uptake and lymphatic transport of HGCO and the normal physiologic changes associated with fat absorption (e.g., lymph flow and an increase in lymphatic endogenous lipids outputs, triglycerides, cholesterol and phospholipids) were similar in the HGCO-and the BO-fed rats. The original differences in gamma-linolenic acid content in HGCO and BO were preserved in the fatty acid composition of the rats' lymph lipid. We conclude that the HGCO derived from the genetically modified canola plant is absorbed and transported into lymph similarly to BO.
Tso
Patrick
P
Department of Pathology, University of Cincinnati Medical Center, Cincinnati, OH 45267, USA. tsopp@email.uc.edu
Ding
Kexi
K
DeMichele
Stephen
S
Huang
Yung-Sheng
YS
D000818:Animals; D001692:Biological Transport; D005227:Fatty Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000493:pharmacokinetics*; D005402:Fistula; D007408:Intestinal Absorption; D050356:Lipid Metabolism; D008196:Lymph / Q000378:metabolism*; D008297:Male; D010938:Plant Oils; D030821:Plants, Genetically Modified; D000074262:Rapeseed Oil; D051381:Rats; D017207:Rats, Sprague-Dawley; D014280:Triglycerides / Q000032:analysis*; D017965:gamma-Linolenic Acid / Q000378:metabolism / Q000493:pharmacokinetics*
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D014280:Triglycerides; D017965:gamma-Linolenic Acid; C079363:borage oil