[A novel immunization schedule for obtaining anti-LPS Haemophilus influenzae type b antibodies].
A novel immunization schedule was tested to increase antibodies to Haemophilus influenzae type B in which a 0.5% formaldehyde-inactivated cellular antigen and a partially purified lipopolysacharid antigen obtained from a selective precipitation with organic solvents were combined as immunogens. High specificity and biological activity antibodies with titers up to 1:256 were obtained by counterinmunoelectrophoresis; they were purified through a selective precipitation with ammonium sulphate and an ion-exchange chromatography using a DEAE-Sapharose column. The method proposed in this paper facilitates the production of purified antibodies useful for the improvement of serological diagnosis and H. influenzae b strain serotyping techniques.
Falcón Márquez
R
R
ciipk@ipk.sld.cu
Toraño Peraza
G
G
Victoria Martínez
B
B
Tamargo Martínez
I
I
D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D019741:Haemophilus influenzae type b / Q000276:immunology*; D007115:Immunization Schedule; D008070:Lipopolysaccharides / Q000276:immunology*
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides