Mapping of genes encoding glycoside hydrolases on the chromosome of Cellulomonas fimi.
Cellulomonas fimi genomic DNA was digested with HpaI, MunI, HindIII, and NsiI, producing fragments ranging in size from 20 to 1400 kbp that were resolved by pulsed field gel electrophoresis. Genetic and physical linkages were determined by Southern blotting and were used to construct a genome map. Cellulomonas fimi has a single circular chromosome of approx. 4000 kbp. Except for two closely linked genes, cbh6A and cel5A, the genes known to encode glycoside hydrolases are scattered widely on the chromosome.
Stoll
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Biotechnology Laboratory and Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada. dominik.stoll@twinstrand.com
D000192:Actinomycetales / Q000235:genetics*; D015139:Blotting, Southern; D002875:Chromosomes / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000032:analysis* / Q000378:metabolism; D004269:DNA, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics*; D008957:Models, Genetic; D020161:Physical Chromosome Mapping; D010957:Plasmids
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D006026:Glycoside Hydrolases