Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology.
A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.
Zhao
Yuqi
Y
Children's Memorial Institute for Education and Research, Northwestern University, Chicago, Illinois 60614, USA. yzhao@northwestern.edu
Yu
Min
M
Miller
Johann W
JW
Chen
Mingzhong
M
Bremer
Eric G
EG
Kabat
William
W
Yogev
Ram
R
D023241:Antiretroviral Therapy, Highly Active; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism
D004279:DNA, Viral; D012367:RNA, Viral; D019914:Taq Polymerase