Translocation t(5;14)(q35;q32) in three cases of childhood T cell acute lymphoblastic leukemia: a new recurring and cryptic abnormality.
We report three cases of T-ALL in which conventional cytogenetic analysis yielded normal karyotypes, but for which a new M-FISH technique (IPM-FISH) was able to detect a translocation. For these patients this technique highlighted a new, recurring and cryptic translocation t(5;14)(q35;q32) in childhood T-ALL which might be phenotypically restricted. The most innovative part of this technique is the use of interspersed polymerase chain reaction (IRS-PCR) painting probes that show an R-band pattern simultaneous with the combinatorial labeling. Contrary to the DOP-PCR, IRS-PCR-derived probes provide stronger hybridization signals at the telomeric ends that potentially increase the possibility of detecting cryptic translocations. All the IPM-FISH findings were validated by FISH with whole chromosome painting and unique sequence probes. These results demonstrate the efficient use of IPM-FISH as an improved, single-step method for the identification of cryptic chromosomal abnormalities. This new IPM-FISH technique is a good tool to display cryptic chromosomal abnormalities.
Hélias
C
C
Laboratoire d'Hématologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
Leymarie
V
V
Entz-Werle
N
N
Falkenrodt
A
A
Eyer
D
D
Costa
J Aurich
JA
Cherif
D
D
Lutz
P
P
Lessard
M
M
D002648:Child; D002675:Child, Preschool; D020223:Chromosome Painting / Q000379:methods*; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics / Q000648:ultrastructure*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics / Q000648:ultrastructure*; D015342:DNA Probes; D006801:Humans; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D016615:Telomere / Q000235:genetics / Q000648:ultrastructure; D014178:Translocation, Genetic
D015342:DNA Probes