title: Microglial derived nitric oxide decreases serotonin content in rat basophilic leukemia (RBL-2H3) cells.
abstract: Nitric oxide (NO) and serotonin (5-hydroxytryptamine; 5-HT) are important neuromodulators that are involved in a myriad of biochemical reactions. In this work, we describe a novel model co-culture system to study the interactions between NO and 5-HT. NO derived from cytokine stimulated Bv2 microglial cells depleted 5-HT from RBL-2H3 cells. Reduction of 5-HT content by NO derived from the NO donor S-nitroso-N-acetylpenicillamine (SNAP) was concentration-dependent, independent of intracellular Ca(2+) and inhibited by reduced glutathione (GSH). Collectively, these data indicate that this cell co-culture system is a viable model to study the mechanisms of interaction between nitrergic and serotonergic pathways.
authors.affiliation: Division of Neuroscience Research in Psychiatry, University of Minnesota, Minneapolis, MN, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Linden
authors.forename: Esam E
authors.initials: EE
authors.lastname: El-Fakahany
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002956:Citrulline / Q000187:drug effects / Q000378:metabolism; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D017628:Microglia / Q000166:cytology / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: C110881:S-nitro-N-acetylpenicillamine; D002956:Citrulline; D009569:Nitric Oxide; D012701:Serotonin; D005978:Glutathione; D010396:Penicillamine; D002118:Calcium