Analytical and semipreparative enantiomeric separation of azole antifungal agents by high-performance liquid chromatography on polysaccharide-based chiral stationary phases. Application to in vitro biological studies.
High-performance liquid chromatography (HPLC) was used for the enantiomeric separation of chiral imidazole derivatives endowed with antimycotic activity. Enantioselective columns, containing carbamates of cellulose and amylose, were used. The influence of the nature and content of an alcoholic modifier in the mobile phase was studied. The isolated enantiomers, separated on semipreparative columns, were submitted to in vitro biological investigations.
Cirilli
R
R
Istituto Superiore di Sanità, Laboratorio di Chimica del Farmaco, Rome, Italy.
Costi
R
R
Di
Santo R
SR
Ferretti
R
R
La
Torre F
TF
Angiolella
L
L
Micocci
M
M
D000935:Antifungal Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D001393:Azoles / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013237:Stereoisomerism
D000935:Antifungal Agents; D001393:Azoles