Determination of tylosin residues in different animal tissues by high performance liquid chromatography.
A HPLC method to determine and quantify tylosin residues from calves, pigs and poultry is reported. This procedure permitted tylosin to be separated from muscle, liver, kidney and fat after a simple extraction with chloroform or ethyl acetate under basic conditions. The analytical methodology showed a high specificity and sensitivity and an adequate precision and accuracy with a limit of quantification of 50 microg/kg. Eight calves were administered 20 mg/kg/day of tylosin for 5 days and slaughtered at 7 and 14 days post-administration. Results showed that at the 14th day tylosin levels were lower than the MRL in all target tissues.
Prats
C
C
Department de Farmacologia i Terapèutica, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain. carme.prats@uab.es
Francesch
R
R
Arboix
M
M
Pérez
B
B
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D011200:Poultry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine; D014018:Tissue Distribution; D015645:Tylosin / Q000032:analysis* / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D015645:Tylosin