[Photodynamic therapy of hematoporphyrin derivative on human pancreatic carcinoma cell lines].
OBJECTIVE
To observe the effect of photodynamic therapy (PDT) on human pancreatic carcinoma cell lines in vitro.


METHODS
Two lines of human pancreatic carcinoma cell P3 and SW1990 were studied to investigate the killing role of PDT with hematoporphyrin derivative (HPD) as photosensitizer and sodium lamp as light source. The cultured cells were treated with serial concentrations of HPD followed by irradiation of different dosages of visible light, then MTT colorimetric assay was applied to measure the relative inhibitory rate of PDT for the cells.


RESULTS
The relative inhibitory rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of light. Under the low photodynamic dose, it increased rapidly, and gradually slowed down to reach the plateau. Under the same photodynamic dose, significant difference in the inhibitory rate between the two lines was observed (P < 0.01), and the LD90 was also different.


CONCLUSIONS
PDT has a significant killing effect on human pancreatic carcinoma cell lines in vitro, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and light irradiation. The sensitivity of the two cell lines to PDT is different.
Zhao
Y
Y
Department of Surgery, Peking Union Medical College Hospital, Beijing 100730, China.
Yang
B
B
Zhang
T
T
Cai
L
L
Zhu
Y
Y
D045744:Cell Line, Tumor; D017324:Hematoporphyrin Derivative / Q000008:administration & dosage; D006414:Hematoporphyrin Photoradiation; D006801:Humans; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017319:Photosensitizing Agents / Q000008:administration & dosage
D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative