A novel endocrine-disrupting agent in corn with mitogenic activity in human breast and prostatic cancer cells.
Housing adult rats on ground corncob bedding impedes male and female mating behavior and causes acyclicity in females. The suppressive effects on ovarian cyclicity are mimicked by a mitogenic agent purified from the ground corncob bedding material (corn mitogen; CM), which stimulates the proliferation of estrogen receptor (ER)-positive (MCF-7 cells) and ER-negative (MDA-MD-231 cells) breast cancer cells. Purified CM does not compete for [(3)H]estradiol binding to ER or nuclear type II sites, and its effects on MCF-7 breast cancer cell proliferation are not blocked by the antiestrogen ICI-182,780. These results suggest that the active component is unlikely to be a phytoestrogen, bioflavonoid, mycotoxin, or other known endocrine-disrupting agent that modifies cell growth via ER or type II [(3)H]estradiol binding sites. CM also stimulates the proliferation of PC-3 human prostatic cancer cells in vitro, and the growth rate of PC-3 cell xenografts is accelerated in nude male mice housed on ground corncob as opposed to pure cellulose bedding. Consequently, this endocrine-disrupting agent in ground corncob bedding may influence behavioral and physiologic reproductive response profiles and malignant cell proliferation in experimental animals. Fresh corn (kernels and cob) or corn tortillas also contain CM, indicating that human exposure is likely; consequently, CM and/or related mitogens in corn products may influence human health and development.
Markaverich
Barry
B
Department of Molecular and Cellular Biology, Center for Comparative Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA. barrym@bcm.tmc.edu
Mani
Shaila
S
Alejandro
Mary Ann
MA
Mitchell
Andrea
A
Markaverich
David
D
Brown
Trellis
T
Velez-Trippe
Claudia
C
Murchison
Chris
C
O'Malley
Bert
B
Faith
Robert
R
D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division; D004703:Endocrine System / Q000187:drug effects; D004971:Estrus; D005260:Female; D005506:Food Contamination; D006799:Housing, Animal; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008934:Mitogens / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000502:physiology; D015203:Reproducibility of Results; D012726:Sexual Behavior, Animal / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014599:Uterus / Q000166:cytology; D003313:Zea mays / Q000737:chemistry*
D008934:Mitogens; D011960:Receptors, Estrogen