[An experimental study of antisense TGF-beta 1 inhibiting keloid fibroblast proliferation in vitro].
OBJECTIVE
To study the effect of antisense TGF-beta 1 on keloid fibroblast proliferation in vitro.


METHODS
The antisense TGF-beta 1 was transfected into the keloid fibroblast by liposome. The cell count technique was used to test the effect of antisense TGF-beta 1 on keloid fibroblast proliferation in vitro. Cell apoptosis was tested by FCM.


RESULTS
Antisense TGF-beta 1 can inhibit the proliferation of keloid fibroblast in vitro; Antisense TGF-beta 1 can increase the apoptosis ratio of keloid fibroblast in vitro.


CONCLUSIONS
Antisense TGF-beta 1 can induce keloid fibroblast apoptosis and inhibit keloid fibroblast proliferation in vitro, which may contribute to the inhibition of keloid formation in vivo.
Shang
Q
Q
Shanghai Institute of Plastic and Reconstructive Surgery, Department of Plastic and Reconstructive Surgery, Shanghai The Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Yuan
R
R
Wang
W
W
D016375:Antisense Elements (Genetics); D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007627:Keloid / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D016375:Antisense Elements (Genetics); C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1