High-throughput screening for biocatalysts.
Recent progress in high-throughput enzyme assays includes new examples of fluorogenic and chromogenic substrates, fluorescence resonance energy transfer substrates, and applications of the pH and pM indicator methods. Recent developments of Horeau's pseudo-enantiomer derivatisation method to screen enantioselectivities in high-throughput have also been reported.
Wahler
D
D
Departement für Chemie und Biochemie, Universität Bern, Freiestrasse 3, 3012 Bern, Switzerland.
Reymond
J L
JL
D001331:Automation; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods; D019281:Dimerization; D004735:Energy Transfer; D004798:Enzymes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D013050:Spectrometry, Fluorescence / Q000379:methods
D004798:Enzymes