title: Intra-amniotic injection of IL-1 induces inflammation and maturation in fetal sheep lung.
abstract: Antenatal inflammation may be an important triggering event in the pathogenesis of bronchopulmonary dysplasia but may also accelerate fetal lung maturation. We examined the effects of intra-amniotic (IA) interleukin (IL)-1 alpha and IL-1 beta on maturation of the fetal sheep lung. These cytokine effects were compared with IA endotoxin, a potent proinflammatory stimulus that accelerated lung maturation. Date-bred ewes received 15 or 150 microg recombinant ovine IL-1 alpha or IL-1 beta or 10 mg Escherichia coli endotoxin by IA injection at 118 days gestation (term = 150 days), and fetuses were delivered at 125 days. IL-1 alpha and IL-1 beta improved lung function and increased alveolar saturated phosphatidylcholine (Sat PC) and surfactant protein mRNA expression at the higher dose. The maturation response to IL-1 alpha was greater than that to IL-1 beta, which was similar to endotoxin response. Inflammation was also more pronounced after IL-1 alpha treatment. Only endotoxin animals had residual inflammation of the fetal membranes at 7 days. Lung compliance, lung volume, and alveolar Sat PC were positively correlated with residual alveolar wash leukocyte numbers 7 days after IL-1 treatment, suggesting a link between lung inflammation and maturation.
authors.affiliation: Division of Clinical Sciences, Center for Child Health Research, Perth, Australia.
authors.forename: Karen E
authors.initials: KE
authors.lastname: Willet
authors.forename: Boris W
authors.initials: BW
authors.lastname: Kramer
authors.forename: Suhas G
authors.initials: SG
authors.lastname: Kallapur
authors.forename: Machiko
authors.initials: M
authors.lastname: Ikegami
authors.forename: John P
authors.initials: JP
authors.lastname: Newnham
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Moss
authors.forename: Peter D
authors.initials: PD
authors.lastname: Sly
authors.forename: Alan H
authors.initials: AH
authors.lastname: Jobe
mesh_terms: D000650:Amnion; D000653:Amniotic Fluid / Q000166:cytology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D036861:Delivery, Obstetric; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005325:Fetal Organ Maturity; D005333:Fetus / Q000187:drug effects / Q000502:physiology; D005544:Forecasting; D007267:Injections; D007375:Interleukin-1 / Q000008:administration & dosage* / Q000494:pharmacology; D007958:Leukocyte Count; D008168:Lung / Q000196:embryology* / Q000502:physiology; D011014:Pneumonia / Q000139:chemically induced*; D011663:Pulmonary Surfactants / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012119:Respiration; D012756:Sheep
chemical_list: D007375:Interleukin-1; D011663:Pulmonary Surfactants; D012333:RNA, Messenger