Integrating waveguide biosensor.
A capillary biosensor is demonstrated which uses the waveguiding properties of the capillary to integrate the signal over an increased surface area without simultaneously increasing the background noise from the detector. This biosensor achieves limits of detection of 30-50 pg/mL in immunoassays using a diode laser for excitation and a PMT for detection. This is approximately 2 orders of magnitude greater sensitivity than was achieved using the same immunoassay reagents in a fiber optic biosensor or a planar array biosensor. Two different approaches to using the capillaries as immunosensors are described, either of which could be adapted for multianalyte sensing.
Ligler
Frances S
FS
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA. fligler@cbmse.nrl.navy.mil
Breimer
Marc
M
Golden
Joel P
JP
Nivens
Delana A
DA
Dodson
James P
JP
Green
Tiffanee M
TM
Haders
Daniel P
DP
Sadik
Omowunmi A
OA
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D005336:Fiber Optic Technology; D007118:Immunoassay / Q000295:instrumentation; D007834:Lasers; D008832:Microchemistry; D055100:Optical Fibers; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity
D011506:Proteins