title: Heme redox properties of S-nitrosated hemoglobin A0 and hemoglobin S: implications for interactions of nitric oxide with normal and sickle red blood cells.
abstract: S-Nitrosated hemoglobin is remarkably stable and can be cycled between deoxy, oxygenated, or oxidized forms without significant loss of NO. Here we show that S-nitrosation of adult human hemoglobin (Hb A(0)) or sickle cell Hb (Hb S) results in an increased ease of anaerobic heme oxidation, while anions cause redox shifts in the opposite direction. The negatively charged groups of the cytoplasmic domain of Band 3 protein also produce an allosteric effect on S-nitrosated Hb. Formation and deoxygenation of a SNO-Hb/Band 3 protein assembly does not in itself cause NO release, even in the presence of glutathione; however, this assembly may play a role in the migration of NO from the red blood cells to other targets and may be linked to Heinz body formation. Studies of the anaerobic oxidation of Hb S revealed an altered redox potential relative to Hb A(0) that favors met-Hb formation and may therefore underlie the increased rate of autoxidation of Hb S under aerobic conditions, the increased formation of Heinz bodies in sickle cells, and the decreased lifetime of red cells containing Hb S. A model for the interrelationships between the deoxy, oxy, and met forms of Hb A(0) and Hb S, and their S-nitrosated counterparts, is presented.
authors.affiliation: Nicholas School of the Environment, Duke University Marine Laboratory, Beaufort, North Carolina 28516-9721, USA.
authors.forename: Celia
authors.initials: C
authors.lastname: Bonaventura
authors.forename: Celine H
authors.initials: CH
authors.lastname: Taboy
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
authors.forename: Robert D
authors.initials: RD
authors.lastname: Stevens
authors.forename: Celine
authors.initials: C
authors.lastname: Lafon
authors.forename: Alvin L
authors.initials: AL
authors.lastname: Crumbliss
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D004913:Erythrocytes, Abnormal / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006451:Hemoglobin, Sickle / Q000378:metabolism*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D006451:Hemoglobin, Sickle; D009569:Nitric Oxide; D006418:Heme