An additional 2'-ribofuranose residue at a specific position of the DNA primer prevents Its elongation by HIV-1 reverse transcriptase.
Oligodeoxynucleotides containing 2'-O-beta-D-ribofuranosyladenosine were prepared and used as modified primers in RNA-templated DNA synthesis catalyzed by HIV reverse transcriptase. It was shown that the additional 2'-ribofuranose residue in specific position of primer prevents its elongation.
Andreeva
O I
OI
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991, Moscow, Russia.
Golubeva
A S
AS
Kochetkov
S N
SN
Van Aerschot
A
A
Herdewijn
P
P
Efimtseva
E V
EV
Ermolinsky
B S
BS
Mikhailov
S N
SN
D020134:Catalytic Domain / Q000187:drug effects; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008958:Models, Molecular; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012266:Ribose; D013329:Structure-Activity Relationship
D017931:DNA Primers; D009841:Oligonucleotides; D012266:Ribose; D054303:HIV Reverse Transcriptase