title: [Genetic subtyping of HIV-1 in Liaoning province of China].
abstract: OBJECTIVE
  To study the prevalence of HIV-1 in Liaoning province of China.
  
  
  METHODS
  Nuclear acids were extracted from blood samples of 16 HIV-1 infected individuals collected locally in Liaoning province of China from Jun. 1997 to Dec. 2000. The 0.7 kb or 1.2 kb segments of HIV-1 env gene were amplified using nested-PCR and the HIV-1 genetic subtypes were then assayed by heteroduplex mobility assay.
  
  
  RESULTS
  Fifteen of 16 samples were positive by PCR amplification of HIV-1 env region and samples were found to be genetic subtype A,B',C,E. The proportion due to sexual transmission in all HIV infection was 31.25% (5/15), among which subtype B' (3/5) was the majority. A man who returned from Africa together with his spouse both had type A (2/5) infection. Intravenous drug users (IDUs) took up 31.25% (5/15) of all the HIV infections. Subtype C (2/4) and E were predominant among intravenous drug users. However, there was one IDU with subtype B or E. Nearly all blood recipients and blood donors were B' (4/5) except one with C.
  
  
  CONCLUSIONS
  There have been several subtypes of HIV-1 existed in Liaoning province, demonstrating the complexity of HIV epidemology in Liaoning province and the difficulty conducting prevention and treatment.
authors.affiliation: P3 Laboratory, 1st Affiliated Hospital China Medical University, Shenyang 110001, China.
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Shang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D000328:Adult; D001803:Blood Transfusion; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012725:Sexual Behavior; D015819:Substance Abuse, Intravenous; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins