Characterization of a new extended-spectrum beta-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey.
A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of blaTEM-87 in Escherichia coli decreased the host susceptibility to these drugs.
Perilli
Mariagrazia
M
Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, L'Aquila, Italy.
Segatore
Bernardetta
B
De Massis
Maria Rosaria
MR
Franceschini
Nicola
N
Bianchi
Ciro
C
Rossolini
Gian Maria
GM
Amicosante
Gianfranco
G
D001398:Aztreonam / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007558:Italy / Q000453:epidemiology; D007700:Kinetics; D008969:Molecular Sequence Data; D008997:Monobactams / Q000494:pharmacology; D011512:Proteus Infections / Q000453:epidemiology* / Q000382:microbiology*; D011513:Proteus mirabilis / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D002511:Cephalosporins; D008997:Monobactams; C456083:beta-lactamase TEM-87; D001618:beta-Lactamases; D001398:Aztreonam