title: Brucella abortus strain RB51 vaccination in elk. I. Efficacy of reduced dosage.
abstract: Bovine brucellosis is a serious zoonotic disease affecting some populations of Rocky Mountain elk (Cervus elaphus nelsoni) and bison (Bison bison) in the Greater Yellowstone Area, USA. The fear that elk and/or bison may spread Brucella abortus to livestock has prompted efforts to reduce or eliminate the disease in wildlife. Brucella abortus strain RB51 (RB51) vaccine has recently been approved for use in cattle. Unlike strain 19 vaccine, RB51 does not cause false positive reactions on standard brucellosis serologic tests. If effective, it may become the vaccine of choice for wildlife. In February 1995, 45 serologically negative female elk calves were trapped and taken to the Sybille Wildlife Research and Conservation Education Unit near Wheatland, Wyoming, USA. In May 1995, 16 of these elk calves were hand-vaccinated with 1 x 10(9) colony forming units (CFU) of RB51, 16 were vaccinated with 1 x 10(8) CFU RB51 by biobullet, and 13 were given a saline placebo. The elk were bred in fall of 1996 and they were challenged with 1 x 10(7) CFU of B. abortus strain 2308 by intraconjunctival inoculation in March 1997. Thirteen (100%) control elk aborted, 14 (88%) hand-vaccinated elk aborted, and 12 (75%) biobullet vaccinated elk aborted or produced nonviable calves. These results suggest that a single dose of 1 x 10(8) to 1 x 10(9) CFU RB51 does not provide significant protection against B. abortus induced abortion in elk. However, the vaccine appears to be safe at this dose and additional study may reveal a more effective RB51 vaccine regimen for elk.
authors.affiliation: Department of Veterinary Science, University of Wyoming, 1174 Snowy Range Road, Laramie, Wyoming 82070, USA. wecook@uwyo.edu
authors.forename: Walter E
authors.initials: WE
authors.lastname: Cook
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Williams
authors.forename: E Tom
authors.initials: ET
authors.lastname: Thorne
authors.forename: Terry J
authors.initials: TJ
authors.lastname: Kreeger
authors.forename: Glen
authors.initials: G
authors.lastname: Stout
authors.forename: Katie
authors.initials: K
authors.lastname: Bardsley
authors.forename: Hank
authors.initials: H
authors.lastname: Edwards
authors.forename: Gerhardt
authors.initials: G
authors.lastname: Schurig
authors.forename: Lesley A
authors.initials: LA
authors.lastname: Colby
authors.forename: Fred
authors.initials: F
authors.lastname: Enright
authors.forename: Philip H
authors.initials: PH
authors.lastname: Elzer
mesh_terms: D000034:Abortion, Veterinary / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D016164:Bison; D002004:Brucella Vaccine / Q000008:administration & dosage* / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary*; D002417:Cattle; D015169:Colony Count, Microbial; D003670:Deer; D004306:Dose-Response Relationship, Immunologic; D005189:False Positive Reactions; D005260:Female; D011247:Pregnancy; D011897:Random Allocation; D016896:Treatment Outcome; D014611:Vaccination / Q000662:veterinary; D015047:Zoonoses
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine