Comparison of blood markers for the longitudinal monitoring of osteoclastic activity in the pig.
In the present study the osteoclast activity was monitored longitudinally in porcine blood samples by measuring the tartrate-resistant acidic phosphatase (TRAP) activity with several methods described for human samples. These methods differed in their specificity for bone-specific TRAP and in their practicability. The validity of TRAP measurements was evaluated by comparison with the peripheral concentrations of the N-terminal fragments of type I collagen with attached cross-links (NTx), a highly bone-specific parameter of bone collagen degradation, using a commercially available test kit developed for human samples. On selected days urine samples were collected for the determination of pyridinium cross-links. The determinations of cross-links in urine were normalized for the creatinine concentrations. However, they were not related to fluoride-sensitive TRAP (fsTRAP) and NTx measurements in serum. The fsTRAP activity in serum, which is assumed to be highly bone-specific, was highly correlated with the NTx concentrations in serum under different experimental conditions. As measurements in blood may be more easily standardized than those in urine, fsTRAP measurements in serum seem to be a highly practicable method to characterize osteoclastic activity in the pig.
Weile
U
U
Universität Hohenheim, Institut für Tierhaltung und Tierzüchtung, Stuttgart, Germany. thsekret@uni-hohenheim.de
Finsler
S
S
Wehr
U
U
Claus
R
R
D000135:Acid Phosphatase / Q000097:blood*; D000596:Amino Acids / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001842:Bone and Bones / Q000378:metabolism*; D003094:Collagen / Q000097:blood*; D024042:Collagen Type I; D007527:Isoenzymes / Q000097:blood*; D008137:Longitudinal Studies; D008297:Male; D010010:Osteoclasts / Q000378:metabolism*; D010455:Peptides / Q000097:blood*; D013552:Swine / Q000097:blood* / Q000254:growth & development / Q000652:urine; D000071681:Tartrate-Resistant Acid Phosphatase
D000596:Amino Acids; D015415:Biomarkers; D024042:Collagen Type I; D007527:Isoenzymes; D010455:Peptides; C052929:collagen type I trimeric cross-linked peptide; C015484:pyridinoline; C036020:deoxypyridinoline; D003094:Collagen; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase