Production, characterization and properties of polysaccharide depolymerizing enzymes from a strain of Curvularia inaequalis.
Xylanase, beta-glucosidase, beta-xylosidase, endoglucanase and polygalacturonase production from Curvularia inaequalis was carried out by means of solid-state and submerged fermentation using different carbon sources. beta-Glucosidase, beta-xylosidase, polygalacturonase and xylanase produced by the microorganisms were characterized. beta-Glucosidase presented optimum activity at pH 5.5 whereas xylanase, polygalacturonase and beta-xylosidase activities were optimal at pH 5.0. Maximal activity of beta-glucosidase was determined at 60 degrees C, beta-xylosidase at 70 degrees C, and polygalacturonase and xylanase at 55 degrees C. These enzymes were stable at acidic to neutral pH and at 40-45 degrees C. The crude enzyme solution was studied for the hydrolysis of agricultural residues.
Gomes
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E
Laboratório de Bioquímica e Microbiologia Aplicada, Universidade Estadual Paulista (UNESP), São José do Rio Preto/São Paulo, 15 054-000 Brasil. eleni@bio.ibilce.unesp.br
Iembo
T
T
Da Silva
R
R
D001203:Ascomycota / Q000201:enzymology* / Q000254:growth & development; D018556:Crops, Agricultural / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011134:Polysaccharides / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D011134:Polysaccharides; D006026:Glycoside Hydrolases