Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography.
High-speed counter-current chromatography was applied to the isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude salvianolic acid B was obtained by extraction with ethanol-water from S. miltiorrhiza Bunge. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (3:7:1:9, v/v) was successfully performed yielding 342 mg salvianolic acid B at 98% purity from 500 mg of the crude extract in a one-step separation.
Li
Hua-Bin
HB
Department of Botany, The University of Hong Kong, China.
Lai
Jia-Ping
JP
Jiang
Yue
Y
Chen
Feng
F
D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D027544:Salvia / Q000737:chemistry*; D053630:Depsides
D001572:Benzofurans; C076944:salvianolic acid B; D053630:Depsides