Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies.
OBJECTIVE
The effect of probiotic lactobacilli is likely dependent on the indigenous Lactobacillus strains in the intestinal tract. Since a substantial number of probiotic studies is performed in rodents, we compared the Lactobacillus strains of different rat and mouse populations in three animal facilities.


RESULTS
SDS-PAGE and 16S rDNA analysis of cultured faecal lactobacilli revealed that different Lactobacillus strains were detected in genetically similar Wistar rats bred at different locations. Further, within the same animal facility host genetics did not affect the types of the predominant lactobacilli strains.


CONCLUSIONS
Our results show that the environmental background of laboratory animals rather than host genetics determines the indigenous Lactobacillus strains that are found.


CONCLUSIONS
Our findings underline the importance of microflora analysis in probiotic studies.
de Waard
R
R
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. waardr@dmv-international.com
Snel
J
J
Bokken
G C A M
GC
Tan
P S T
PS
Schut
F
F
Huis In't Veld
J H J
JH
D000818:Animals; D000830:Animals, Laboratory / Q000235:genetics; D001947:Breeding; D004275:DNA, Ribosomal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D006799:Housing, Animal; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D010802:Phylogeny; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S