title: Preliminary analysis of the mouse cerebellum proteome.
abstract: This paper reports on the initial analysis of protein expression in the mouse cerebellum with the proteomics approach. Proteins from cerebellar tissue homogenates were separated by two-dimensional gel electrophoresis, and the proteins were stained with colloidal Coomassie Blue to produce a high-resolution map of the cerebellum proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography-electrospray quadrupole ion trap mass spectrometry. The mass spectrometric data were used to identify the proteins through searches of the SWISSPROT protein sequence database. To date, 30 prominent proteins with various functional characteristics were identified. These data will be used for future studies of differential protein expression in mouse models of neurological disorders.
authors.affiliation: The Charles B. Stout Neuroscience Mass Spectrometry Laboratory, The University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: Sarka
authors.initials: S
authors.lastname: Beranova-Giorgianni
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pabst
authors.forename: Tara M
authors.initials: TM
authors.lastname: Russell
authors.forename: Francesco
authors.initials: F
authors.lastname: Giorgianni
authors.forename: Daniel
authors.initials: D
authors.lastname: Goldowitz
authors.forename: Dominic M
authors.initials: DM
authors.lastname: Desiderio
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000737:chemistry*; D004396:Coloring Agents; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice / Q000235:genetics / Q000378:metabolism*; D008815:Mice, Inbred Strains; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D010449:Peptide Mapping; D020543:Proteome; D012394:Rosaniline Dyes; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004396:Coloring Agents; D009419:Nerve Tissue Proteins; D020543:Proteome; D012394:Rosaniline Dyes; C048139:Coomassie blue