Anti-hepatoma activity of resveratrol in vitro.
OBJECTIVE
To study the anti-tumor effect of resveratrol alone and the synergistic effects of resveratrol with 5-FU on the growth of H22 cells line in vitro.


METHODS
The number of cells was measured by MTT method the morphological changes of H22 cells were investigated under microscopy and electron microscopy examination.


RESULTS
Resveratrol inhibited the growth of hepatoma cells line H22 in a dose- and time-dependent manner, IC50 of the resveratrol on H22 cells was 6.57mg x L(-1),The synergistic anti-tumor effects of resveratrol with 5-FU increased to a greater extent than for H22 cells treated with 5-FU alone (70.2% vs 28.4%) P<0.05 .Under microscope and electron microscope, characteristics of apoptosis such as typical apoptotic bodies were commonly found in tumor cells in the drug-treated groups.


CONCLUSIONS
Resveratrol can suppresses the growth of H22 cells in vitro,its anti-tumor activity may occur through the induction of apoptosis.
Sun
Zhong-Jie
ZJ
Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi Province, China. szhjie@21cn.com
Pan
Cheng-En
CE
Liu
Hong-Shan
HS
Wang
Guo-Jun
GJ
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes; D000077185:Resveratrol