title: Mrnp41 (Rae 1p) associates with microtubules in HeLa cells and in neurons.
abstract: Mrnp41 (hRae1p) is an evolutionarily highly conserved protein, which is a potential component of mRNP particles and plays a role in nuclear mRNA export. The protein is mainly localized at the nuclear pore complex, but is also associated with distinct nuclear domains and with a meshwork of numerous small particles in the cytoplasm (Kraemer and Blobel (1997): Proc. Natl. Acad. Sci. USA 91, 1519-1523). We show that the cytoplasmic pattern of mrnp41 is sensitive to treatment with the microtubule (MT)-depolymerizing drug nocodazole which causes disappearance of mrnp41 from the cell periphery and concentration around the nucleus. By immunofluorescence we demonstrate that mrnp41 colocalizes with MT in HeLa cells and displays an MT-like distribution in cultured neurons. Association of mrnp41 with MT is further demonstrated by copurification with MT from pig brain throughout several steps of polymerization and depolymerization. Separation of MT-associated proteins (MAPs) by phosphocellulose (PC) chromatography showed copurification of mrnp41 with MAPs. These data show an association of mrnp41 with MT and, moreover, demonstrate that an intact MT system is necessary for dispersion of mrnp41-containing particles to the cellular periphery. The essential role of mrnp41 in spindle pole separation and cell cycle progression may also be related to its ability to bind to MTs.
authors.affiliation: Medizinische Poliklinik, Julius-Maximilians Universität, Würzburg, Germany. d.kraemer@medizin.uni-wuerzburg.de
authors.forename: D
authors.initials: D
authors.lastname: Kraemer
authors.forename: T
authors.initials: T
authors.lastname: Dresbach
authors.forename: D
authors.initials: D
authors.lastname: Drenckhahn
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism / Q000648:ultrastructure; D002453:Cell Cycle; D002478:Cells, Cultured; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D034521:Nuclear Matrix-Associated Proteins; D029543:Nucleocytoplasmic Transport Proteins; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
chemical_list: D034521:Nuclear Matrix-Associated Proteins; D029543:Nucleocytoplasmic Transport Proteins; C490522:RAE1 protein, human; D016601:RNA-Binding Proteins