Wdr12, a mouse gene encoding a novel WD-Repeat Protein with a notchless-like amino-terminal domain.
The WD-repeat protein family consists of a large group of structurally related yet functionally diverse proteins found predominantly in eukaryotic cells. These factors contain several (4-16) copies of a recognizable amino-acid sequence motif (the WD unit) thought to be organized into a "propeller-like" structure involved in protein-protein regulatory interactions. Here, we report the cloning of a mouse cDNA, referred to as Wdr12, which encodes a novel WD-repeat protein of 423 amino acids. The WDR12 protein was predicted to contain seven WD units and a nuclear localization signal located within a protruding peptide between the third and fourth WD domains. The amino-terminal region shows similarity to that of the Notchless WD repeat protein. Sequence comparisons revealed WDR12 orthologs in various eukaryotic species. Wdr12 seems to correspond to a single-copy gene in the mouse genome, located within the C1-C2 bands of chromosome 1. These data, together with the results of Wdr12 gene expression studies and evidence of in vitro binding of WDR12 to the cytoplasmic domain of Notch1, led us to postulate a function for the WDR12 protein in the modulation of Notch signaling activity.
Nal
Béatrice
B
Centre d'Immunologie de Marseille-Luminy (CIML), INSERM-CNRS-Université de la Méditerranée, 13288 Marseille, France.
Mohr
Elodie
E
Silva
Maria-Isabel Da
MI
Tagett
Rebecca
R
Navarro
Christel
C
Carroll
Patrick
P
Depetris
Danielle
D
Verthuy
Christophe
C
Jordan
Bertrand R
BR
Ferrier
Pierre
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D020449:Repetitive Sequences, Amino Acid / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; C117007:Nle protein, Drosophila; D011506:Proteins; D016601:RNA-Binding Proteins; C454694:Wdr12 protein, mouse