The CFTR-mediated protein secretion defect: pharmacological correction.
The cystic fibrosis transmembrane conductance regulator (CFTR) mediates secretion of mucins and serous proteins. The aim was to correct pharmacologically the CFTR defect in protein secretion in airway gland cells and so to correct the viscous mucous secretions in cystic fibrosis (CF) airways and lungs. The strategies tested included direct activation of CFTR, bypass of CFTR-mediated protein secretion and movement of the mutated form of CFTR (DeltaF(508)-CFTR) to the cell membrane. Compounds related to 3-isobutyl-1-methylxanthine (IBMX), including a selective type-IV phosphodiesterase inhibitor and the adenosine receptor antagonists 8-cyclopentyltheophylline (CPT) and 8-cyclopentyl-1,3-dipropylxanthine (CPX), corrected the defective beta-adrenergic stimulation of mucin secretion in CFTR antibody-inhibited submandibular gland cells. CPT also corrected lactoferrin secretion in DeltaF(508)/DeltaF(508)-CFTR nasal gland cells. The data suggest that correction of CFTR protein secretion activity is not mediated by excessive increase in cyclic AMP, involves direct interaction with CFTR but does not require increase in CFTR Cl(-) channel activity. Regulated glycoprotein secretion was characterised in the airway gland cell line Calu-3 to investigate whether a CFTR bypass is present. Studies of DeltaF(508)-CFTR trafficking using confocal imaging showed that some DeltaF(508)-CFTR colocalised with the apical membrane protein CD59; however a large amount was mislocalised within the cell. The results showing pharmacological correction of the defective CFTR-mediated protein secretion afford promise for the development of a rational drug therapy for CF patients.
McPherson
M A
MA
Department of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK.
Pereira
M M
MM
Russell
D
D
McNeilly
C M
CM
Morris
R M
RM
Stratford
F L
FL
Dormer
R L
RL
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000188:drug therapy* / Q000378:metabolism*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000032:analysis / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007781:Lactoferrin / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D009297:Nasal Mucosa / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013363:Submandibular Gland / Q000378:metabolism; D013806:Theophylline / Q000031:analogs & derivatives* / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology*
D000318:Adrenergic beta-Agonists; C505032:CFTR protein, human; D004791:Enzyme Inhibitors; D009077:Mucins; D013873:Thionucleotides; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C053907:8-cyclopentyl-1,3-dimethylxanthine; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D013806:Theophylline; D000242:Cyclic AMP; D007781:Lactoferrin; D007545:Isoproterenol