Isolation of a new cyclophilin-like protein from chickpeas with mitogenic, antifungal and anti-HIV-1 reverse transcriptase activities.
A protein designated chickpea cyclophilin-like antifungal protein (CLAP) was isolated from seeds of the chickpea (Cicer arietinum). Chickpea CLAP was characterized by a molecular weight of 18 kDa and an N-terminal sequence homologous to cyclophilins. The protein was isolated with a procedure involving affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose. In addition to an inhibitory effect on the growth of fungi including Rhizoctonia solani, Mycosphaerella arachidicola and Botrytis cinerea, the protein was capable of inhibiting human immunodeficiency virus-1 reverse transcriptase. Chickpea CLAP did not possess lectin and ribonuclease activities but it weakly inhibited translation in a rabbit reticulocyte lysate system. The protein stimulated 3H-thymidine incorporation by mouse splenocytes.
Ye
X Y
XY
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, China.
Ng
T B
TB
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification*; D029863:Cicer / Q000737:chemistry*; D021983:Cyclophilins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D054303:HIV Reverse Transcriptase / Q000187:drug effects*; D008934:Mitogens / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D018894:Reverse Transcriptase Inhibitors / Q000302:isolation & purification*
D000935:Antifungal Agents; D008934:Mitogens; D010940:Plant Proteins; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase; D021983:Cyclophilins