Application of random amplified polymorphic DNA (RAPD) analysis for identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets.
A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
Asensio
Luis
L
Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
González
Isabel
I
Fernández
Alicia
A
Rodríguez
Miguel A
MA
Lobo
Esther
E
Hernández
Pablo E
PE
García
Teresa
T
Martín
Rosario
R
D000818:Animals; D017931:DNA Primers; D005399:Fishes / Q000235:genetics*; D005515:Food Labeling; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017747:Seafood / Q000592:standards*; D013045:Species Specificity
D017931:DNA Primers