title: Isolation, characterization and diuron transformation capacities of a bacterial strain Arthrobacter sp. N2.
abstract: A bacterial strain able to transform diuron was isolated from a soil by enrichment procedures. Strain isolation was realized by plating on minimal-agarose medium spread with this herbicide and selecting the colonies surrounded by a clear thin halo. One strain was characterized and identified as an Arthrobacter sp. It metabolized diuron and the final transformation product, 3,4-dichloroaniline, was produced in stoichiometric amounts. The transformation of diuron at different concentrations was more efficient in the presence of alternative sources of carbon and nitrogen. The bacterial activity was also evaluated in soil microcosms with a consequent disappearance of diuron and concomitant appearance of 3,4-dichloroaniline, of which the concentration decreased thereafter. Bacterial cells inoculated in the microcosms survived as viable but eventually nonculturable cells.
authors.affiliation: Laboratoire de Génétique Microbienne, UMR 6022, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Widehem
authors.forename: Selim
authors.initials: S
authors.lastname: Aït-Aïssa
authors.forename: Céline
authors.initials: C
authors.lastname: Tixier
authors.forename: Martine
authors.initials: M
authors.lastname: Sancelme
authors.forename: Henri
authors.initials: H
authors.lastname: Veschambre
authors.forename: Nicole
authors.initials: N
authors.lastname: Truffaut
mesh_terms: D001173:Arthrobacter / Q000502:physiology*; D001673:Biodegradation, Environmental; D001711:Biotransformation; D004237:Diuron / Q000737:chemistry / Q000378:metabolism*; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D006540:Herbicides; D012989:Soil Pollutants; D004237:Diuron