[Effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice].
OBJECTIVE
To investigate the effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice (Renca).


METHODS
The model of renal tumor-bearing mice was constructed by subcutaneous injection of Renca cells. The tumor-bearing mice were divided into 5 groups at random three days after injection of Renca cells. Different groups of mice were given different treatments. The antitumor immune responses and the survival time of mice were tested. Histopathologic analysis of tumor tissue was done.


RESULTS
Enhancement of antitumor immune responses and prolongation of survival period were observed more obviously in tumor-bearing mice after combined therapy than in those of the other four groups.


CONCLUSIONS
p16 and GM-CSF combined gene therapy can enhance the antitumor immune responses of mice and inhibit the growth of pre-established renal cell carcinoma.
Wang
L
L
Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Sun
Y
Y
Yang
Z
Z
Qian
S
S
Zheng
J
J
Ma
Y
Y
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D019942:Genes, p16; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D007680:Kidney Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
D016178:Granulocyte-Macrophage Colony-Stimulating Factor