Scintigraphic imaging using 99mTc-labeled PEG liposomes allows early detection of experimental invasive pulmonary aspergillosis in neutropenic rats.
The value of scintigraphic imaging using 99mTc labeled poly(ethyleneglycol) (PEG) -liposomes for detecting invasive pulmonary aspergillosis at different stages of the disease was investigated in a rat model. At 24, 48, 72, 120 and 168 h after fungal inoculation scintigraphic images were obtained and biodistribution of the radiolabel was determined. Findings were compared with serum galactomannan detection and other parameters of progression of fungal infection. At 48 h liposomal uptake in the infected left lung was increased significantly and 82% of the scintigraphic images was assessed positive. Serum galactomannan was only detected at 72 h and later. Liposomal uptake in the infected left lung increased over time and was significantly correlated with both the size of the pulmonary hemorrhagic lesion and the levels of circulating galactomannan. It was concluded that scintigraphic imaging using 99mTc-PEG-liposomes allows early detection of invasive pulmonary aspergillosis in this model, and that liposomal uptake in the infected lung was strongly associated with the severity of the disease.
Becker
Martin J
MJ
Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GE Rotterdam, 50, The, Dr Molewaterplein, Netherlands. becker@kmic.fgg.eur.nl
Dams
Els Th M
ET
de Marie
Siem
S
Oyen
Wim J G
WJ
Boerman
Otto C
OC
Fens
Marcel H A M
MH
Verbrugh
Henri A
HA
Bakker-Woudenberg
Irma A J M
IA
D000818:Animals; D001228:Aspergillosis / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D005690:Galactose / Q000031:analogs & derivatives; D007668:Kidney / Q000378:metabolism; D008081:Liposomes / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging* / Q000473:pathology; D008351:Mannans / Q000097:blood; D009503:Neutropenia / Q000150:complications*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D013154:Spleen / Q000378:metabolism; D013667:Technetium / Q000493:pharmacokinetics; D014018:Tissue Distribution
D008081:Liposomes; D008351:Mannans; D019275:Radiopharmaceuticals; C012990:galactomannan; D011092:Polyethylene Glycols; D013667:Technetium; D005690:Galactose