Ultra-low-temperature non-aqueous capillary electrophoretic separation--77 K fluorescence spectroscopic detection for the on-line identification of photo-converted analytes of trans-resveratrol.
We demonstrate here, for the first time, that non-aqueous capillary electrophoresis (NACE) can be interfaced with any ultra-low-temperature (ULT) separation method and 77 K fluorescence spectroscopy (FS). This novel ULT-NACE-FS system consists of a modular CE system, a dry ice bath, and instrumentation for 77 K fluorescence detection. The ULT-NACE method serves to separate structurally similar molecules by a combination of a low electrophoresis current and a high voltage at approximately -70 degrees C. When the ULT-NACE-separated analytes move into the quartz Dewar flask and traverse into the capillary detection window, liquid nitrogen was added, thus freezing the separating analyte zones, allowing the collection of 77 K fluorescence spectra for on-line spectral fingerprint identification. The first application of the ULT-NACE-FS system is described for the analysis of photo-converted analytes of trans-resveratrol; prospects and future applications of ULT-NACE-FS are also briefly addressed.
Chen
Yi-Hsiu
YH
Department of Chemistry, National Taiwan Normal University, Taipei.
Chung
Yu-Lin
YL
Lin
Cheng-Huang
CH
D003080:Cold Temperature; D019075:Electrophoresis, Capillary / Q000379:methods*; D010777:Photochemistry; D000077185:Resveratrol; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013267:Stilbenes / Q000032:analysis*
D013267:Stilbenes; D000077185:Resveratrol