title: UVB-activated psoralen reduces luminal narrowing after balloon dilation because of inhibition of constrictive remodeling.
abstract: In this study we have explored the potential of PUVB (8-MOP + UVB) therapy for the reduction of luminal narrowing after arterial injury. In 15 rabbits, balloon dilation of iliac arteries was performed. In 20 arteries, dilation was combined with the delivery of pulsed ultraviolet light B (UVB) irradiation with 10 arteries being previously subjected to sensitizer infusion. Changes in vessel diameter, proliferation and extracellular matrix protein content at 6 weeks were evaluated by means of angiography and histomorphometry-immunohistochemistry. We found that PUVB, applied at the time of dilation, induced reduction in late loss (LL) at 6 weeks (percutaneous transluminal angioplasty vs UVB vs PUVB: 0.64 +/- 0.15 mm vs 0.61 +/- 0.05 mm vs 0.29 +/- 0.05 mm; p = 0.018). The same holds true for constrictive remodeling (0.53 +/- 0.15 mm vs 0.45 +/- 0.06 mm vs 0.15 +/- 0.05 mm; p = 0.016). In the irradiation groups, LL was independent of acute gain (AG), as opposed to the control. Collagen content increased significantly after PUVB in media and adventitia, without increased cellular proliferation in all vessel layers. Thus, PUVB at the time of dilation reduced luminal narrowing at follow-up without effecting proliferation. This effect was independent of AG and was associated with increased collagen content in media and adventitia.
authors.affiliation: Department of Cardiology, Heart-Lung Institute, University Medical Center Utrecht, The Netherlands.
authors.forename: Jop
authors.initials: J
authors.lastname: Perrée
authors.forename: Ton G
authors.initials: TG
authors.lastname: van Leeuwen
authors.forename: Evelyn
authors.initials: E
authors.lastname: Velema
authors.forename: Mirjam
authors.initials: M
authors.lastname: Smeets
authors.forename: Dominique
authors.initials: D
authors.lastname: de Kleijn
authors.forename: Cornelius
authors.initials: C
authors.lastname: Borst
mesh_terms: D015906:Angioplasty, Balloon, Coronary; D000818:Animals; D020212:Carotid Artery Injuries / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D002404:Catheterization; D003094:Collagen / Q000378:metabolism; D023903:Coronary Restenosis / Q000517:prevention & control; D011564:Furocoumarins / Q000627:therapeutic use; D011701:PUVA Therapy; D018462:Photobiology; D011817:Rabbits; D014467:Ultraviolet Therapy
chemical_list: D011564:Furocoumarins; D003094:Collagen