Photofrin--factor of photodynamic therapy induces apoptosis and necrosis.
Photodynamic therapy (PDT) causes irreversible photodamage of tumor and other malignant tissues. The effect of reactive oxygen species generation in the presence of photofrin (HpD) was studied. The studies were performed on endothelial cell line from foetal aorta of calves and on normal fibroblasts cell line (3T3 -Balb) and also on malignant line (A431). The cells were grown in presence of photofrin at different time intervals. Time of interaction of photosensitiser with cells was very important. Short time of exposure of the cells to photofrin induced mostly apoptosis in normal cells and apoptotic or necrotic changes in malignant cells. Longer effect of these factors on cells provoked necrosis. The factors of PDT influence dynamic changes of SOD and CT activity. It was dependent on the intensity of factors. These results strongly suggest that HpD has an effect on generation of ROS, which are a signal for development of morphological changes (apoptosis or necrosis) in normal and malignant cells.
Marcinkowska
A
A
Departmentsof Medical Biochemistry, University of Medicine, Wrocław, Poland.
Malarska
A
A
Saczko
J
J
Chwiłkowska
A
A
Wysocka
T
T
Drag-Zalesińska
M
M
Wysocka
T
T
Banaś
T
T
D000818:Animals; D001011:Aorta / Q000166:cytology; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D003879:Dermatologic Agents / Q000494:pharmacology*; D017323:Dihematoporphyrin Ether / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009336:Necrosis; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D003879:Dermatologic Agents; D017382:Reactive Oxygen Species; D017323:Dihematoporphyrin Ether; D002374:Catalase; D013482:Superoxide Dismutase