[The isolation and characterization of dentin phosphoprotein from human dentine].
OBJECTIVE
To establish the method of isolation and characterization of dentin phosphoprotein (DPP) from human dentine.


METHODS
Dentin powder was placed in dialysis tube after demineralized to completion with 0.6 mol/L HCl, then isolated DPP through DEAE-Sepharose CL6B anion exchange chromatography. Its protein composition, organic phosphorus content, molecular weight and amino acid were analyzed.


RESULTS
DPP contained high organic phosphorus, 23.8% of aspartic residue and 19.1% serine residue; their molecular weight were 141, 000, 124, 000, 108, 000 respectively.


CONCLUSIONS
DPP can be extracted from human dentine with 0.6 mol/L HCl demineralization. and DEAE-Sepharose CL6B anion exchange chromatography. It is a group of proteins which have a high content of organic phosphorus and is found to be rich in serine and aspartic acid residues.
Ouyang
Y
Y
Beijing Stomatological Hospital, Capital University of Medical Sciences, Beijing 100050.
Li
Y
Y
Su
Y
Y
D000596:Amino Acids / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification*; D010758:Phosphorus / Q000032:analysis
D000596:Amino Acids; D010750:Phosphoproteins; D010758:Phosphorus