Effects of HgCl(2) on CO(2) dependence of leaf photosynthesis: evidence indicating involvement of aquaporins in CO(2) diffusion across the plasma membrane.
Experiments were conducted to examine whether mercury-sensitive aquaporins facilitate photosynthetic CO(2) diffusion across the plasma membrane of leaf mesophyll cells. Discs without abaxial epidermes from Vicia faba leaflets were treated with HgCl(2), an inhibitor of aquaporins. Hydraulic conductivity of the plasma membrane of these discs, measured as the weight loss of the discs in the 1 M sorbitol solution, was inhibited by sub-mM concentrations of HgCl(2) by 70 to 80%. Photosynthetic CO(2) fixation was also inhibited by the HgCl(2) treatment in a similar concentration range. When 0.3 mM HgCl(2) solution was fed to the V. faba leaflets with intact epidermes via the transpiration stream, the rate of photosynthesis on leaf area basis (A) measured at photosynthetically active photon flux density of 700 micromol m(-2) s(-1) and at leaf temperature of 25 degrees C, decreased by about 20 to 30% at any CO(2) concentration in the intercellular spaces (C(i)). However, when CO(2) concentration in the chloroplast stroma (C(c)) was calculated from fluorescence and gas exchange data and A was plotted against C(c), A at low C(c) concentrations did not differ before and after the treatment. The conductance for CO(2) diffusion from the intercellular spaces to the chloroplast stroma (g(i)) decreased to 40 and 30% of the control value, when the leaflets were fed with 0.3 mM and 1.2 mM HgCl(2), respectively. Similar results were obtained with leaves of Phaseolus vulgaris. Although effects of HgCl(2) were not specific, the present results showed that HgCl(2) consistently lowered g(i). It is, thus, probable that the photosynthetic CO(2) uptake across the plasma membrane of the mesophyll cells is facilitated by mercury-sensitive aquaporins.
Terashima
Ichiro
I
Department of Biology, Graduate School of Science, Osaka University, 1-16 Machikaneyama-cho, Toyonaka, Osaka, 560-0043 Japan. itera@bio.sci.osaka-u.ac.jp
Ono
Kiyomi
K
D000465:Algorithms; D020346:Aquaporins / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002245:Carbon Dioxide / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism; D005110:Extracellular Space / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects / Q000378:metabolism; D017669:Mercury Compounds / Q000494:pharmacology*; D010788:Photosynthesis / Q000187:drug effects* / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D014867:Water / Q000378:metabolism
D020346:Aquaporins; D017669:Mercury Compounds; D014867:Water; D002245:Carbon Dioxide; C015728:calomel