title: Tumour necrosis factor-alpha-induced migration of human Langerhans cells: the influence of ageing.
abstract: BACKGROUND
  Langerhans cells (LCs) play essential roles in the initiation and regulation of cutaneous immune responses mediated through their successful migration from the epidermis to draining lymph nodes while carrying antigen. Tumour necrosis factor (TNF)-alpha, a keratinocyte-derived cytokine, has recently been shown to play an important role in the mobilization of LCs from human epidermis. Although it is known that with age the immune system changes, the influence of increasing age on the function of human LCs has not been defined clearly.
  
  
  OBJECTIVE
  To examine the influence of age on the ability of TNF-alpha to induce LC migration.
  
  
  METHODS
  Ten elderly (six men, four women; mean age 76 years, range 72-79) and 10 young (six men, four women; mean age 23 years, range 18-35) volunteers received intradermal injections of 200 U of human recombinant TNF-alpha diluted in sterile saline, and control injections of sterile saline alone, at each of two paired sites identified on photoprotected buttock skin. Two hours later, paired injection sites were excised by punch biopsy. One set of paired biopsies was processed for assessment of the frequency and morphology of epidermal LCs, following preparation of epidermal sheets and immunofluorescence staining for the LC marker CD1a. The remaining paired biopsies were processed in formalin and the inflammatory response to TNF-alpha was assessed by standard histological examination.
  
  
  RESULTS
  Mean +/- SEM baseline values for LC frequency within epidermal sheets were significantly different between young (1156.3 +/- 38.5 cells mm(-2)) and elderly subjects (835.7 +/- 48.2 cells mm(-2); P < 0.01). Intradermal injections of 200 U of TNF-alpha caused a significant reduction in the frequency of LCs in both elderly and young subjects (P < 0.01). However, the extent of TNF-alpha-induced LC migration was substantially different between the two groups, with a mean 9% reduction in LC frequency in elderly volunteers compared with a mean 23% decrease in young subjects. Exposure to TNF-alpha was associated with a perivascular polymorphonuclear infiltrate at 2 h in all young subjects; in contrast, only 50% of the elderly individuals showed evidence of such a response.
  
  
  CONCLUSIONS
  There are significant differences between young and old skin with respect to both resting LC numbers and their response to TNF-alpha. These age-related changes in LC frequency and function may contribute to the altered cutaneous immune function observed in the elderly.
authors.affiliation: Dermatopharmacology Unit, The Dermatology Centre, University of Manchester, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bhushan
authors.forename: M
authors.initials: M
authors.lastname: Cumberbatch
authors.forename: R J
authors.initials: RJ
authors.lastname: Dearman
authors.forename: S M
authors.initials: SM
authors.lastname: Andrew
authors.forename: I
authors.initials: I
authors.lastname: Kimber
authors.forename: C E M
authors.initials: CE
authors.lastname: Griffiths
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D003872:Dermatitis / Q000503:physiopathology; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D007801:Langerhans Cells / Q000502:physiology*; D008297:Male; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D014409:Tumor Necrosis Factor-alpha