Platelet shape changes and adhesion under high shear flow.
Recent studies have revealed that the platelet adhesive process under flow is tightly regulated by multiple ligand-receptor interactions. However, platelet morphological changes during this process, particularly its physiological relevance, remain unknown under blood flow conditions. Using epifluorescence and scanning electron microscopy, we evaluated the real-time changes in platelet morphology during a platelet adhesive process on a von Willebrand factor-coated surface under physiological high shear flow in a perfusion chamber. Here, we show that dynamic platelet shape changes occurring during distinct phases of the adhesive process are precisely regulated by "inside-out" and "outside-in" integrin signals and are also a key regulatory element in successful platelet thrombogenesis opposing rapid blood flow in vivo.
Kuwahara
Mitsuhiro
M
Department of Pediatrics, Nara Medical University, Kashihara, Nara, Japan.
Sugimoto
Mitsuhiko
M
Tsuji
Shizuko
S
Matsui
Hideto
H
Mizuno
Tomohiro
T
Miyata
Shigeki
S
Yoshioka
Akira
A
D000730:Androstadienes / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D010875:Pipecolic Acids / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013314:Stress, Mechanical; D013449:Sulfonamides; D000077191:Wortmannin
D000730:Androstadienes; D010875:Pipecolic Acids; D013449:Sulfonamides; D004533:Egtazic Acid; C058180:5,5'-dimethyl-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate; D001120:Arginine; C031942:argatroban; D002118:Calcium; D000077191:Wortmannin