title: [Experimental study of the transferred keratinocytes transplanted on biomembrane].
abstract: OBJECTIVE
  To search an ideal carrier of transferred keratinocytes for transplantation.
  
  
  METHODS
  The transferred keratinocytes were seeded on the surfaces of the artificial dermis and the silicone membrane and cultured in vitro for 2 weeks. The growth of the keratinocytes was observed by microscope and scanning electron microscope.
  
  
  RESULTS
  The keratinocytes implanted on the artificial dermis began to rupture and died after 2 to 3 days. While the keratinocytes adhered well on the surface of silicone membrane with pseudopodia formation after 1 week under scanning electron microscope, and the cells kept normal morphological and proliferative properties 2 weeks later.
  
  
  CONCLUSIONS
  The silicone membrane can be applied as an useful carrier for the keratinocytes transplantation.
authors.affiliation: Department of Burns and Plastic Surgery, Shanghai Corps Hospital, Chinese People's Armed Police Force, Shanghai, P. R. China 201103.
authors.forename: Zhao-yang
authors.initials: ZY
authors.lastname: Dong
authors.forename: Yu-lin
authors.initials: YL
authors.lastname: Chen
authors.forename: Yeurgen
authors.initials: Y
authors.lastname: Kopp
authors.forename: Wen-kui
authors.initials: WK
authors.lastname: Zhang
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Yu
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: zhi-yang
authors.initials: zY
authors.lastname: Fang
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D008567:Membranes, Artificial; D012828:Silicones; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods
chemical_list: D008567:Membranes, Artificial; D012828:Silicones