title: Characterization of a new extended-spectrum beta-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey.
abstract: A new natural TEM derivative, named TEM-87, was identified in a Proteus mirabilis isolate from an Italian hospital. Compared to TEM-1, TEM-87 contains the following mutations: E104K, R164C, and M182T. Kinetic analysis of TEM-87 revealed extended-spectrum activity against oxyimino cephalosporins (preferentially ceftazidime) and aztreonam. Expression of blaTEM-87 in Escherichia coli decreased the host susceptibility to these drugs.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, L'Aquila, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: De Massis
authors.forename: Nicola
authors.initials: N
authors.lastname: Franceschini
authors.forename: Ciro
authors.initials: C
authors.lastname: Bianchi
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D001398:Aztreonam / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007558:Italy / Q000453:epidemiology; D007700:Kinetics; D008969:Molecular Sequence Data; D008997:Monobactams / Q000494:pharmacology; D011512:Proteus Infections / Q000453:epidemiology* / Q000382:microbiology*; D011513:Proteus mirabilis / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002511:Cephalosporins; D008997:Monobactams; C456083:beta-lactamase TEM-87; D001618:beta-Lactamases; D001398:Aztreonam