Chlorogentisylquinone, a new neutral sphingomyelinase inhibitor, produced by a marine fungus.
Chlorogentisylquinone, a new inhibitor of neutral sphingomyelinase activity, was purified from the culture broth of a fungal strain FOM-8108 isolated from a marine environment by solvent extraction, silica gel chromatography and Sephadex LH-20 chromatography. Its chemical structure was elucidated by spectroscopic studies including 1H, 13C, DEPT, HMQC and HMBC NMR experiments. Chlorogentisylquinone inhibited neutral sphingomyelinase activity of rat brain membranes with an IC50 value of 1.2 microM.
Uchida
R
R
Kitasato Institute for Life Sciences, Kitasato University and The Kitasato Institute, Tokyo, Japan.
Tomoda
H
H
Arai
M
M
Omura
S
S
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005285:Fermentation; D005658:Fungi / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification*; D013108:Sphingomyelin Phosphodiesterase / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D011809:Quinones; C447409:gentisylquinone; C447440:chlorogentisylquinone; D013108:Sphingomyelin Phosphodiesterase