title: Early activation of hepatic stellate cells and perisinusoidal extracellular matrix changes during ex vivo pig liver perfusion.
abstract: In previous works, we observed during liver transplantation procedure, the early activation of hepatic stellate cells (HSC) which acquire alpha-smooth muscle (SM) actin expression. In this study, we evaluated changes in HSC and in perisinusoidal extracellular matrix during ex vivo pig liver perfusion. Under general anesthesia, pig livers were flushed and removed, and then perfused ex vivo for 6 h with homologous blood. Liver biopsies were taken before and after washout, at 5 min perfusion, and then hourly. Tissues were processed for immunohistochemistry, immunofluorescence, confocal microscopy, in situ hybridization and electron microscopy. Before and after liver washout, alpha-SM actin was present in vessel walls but in very few lobular HSC. After 1 h perfusion, a strong reactivity for alpha-SM actin was present in HSC, particularly along dilated sinusoids. At the ultrastructural level, numerous microfilament bundles appeared in HSC cytoplasmic processes. During perfusion, type I and type IV collagens, type III procollagen, and fibronectin acquired a looser organisation in relation with the enlargement of perisinusoidal spaces; laminin appeared in perisinusoidal spaces around portal areas and fibrillin deposits increased. In situ hybridization studies showed an increase of the type I procollagen mRNA expression mainly in portal tracts and septa. Ex vivo liver perfusion induces: 1) an early activation of HSC which acquire the expression of alpha-SM actin, and 2) significant changes in the perisinusoidal extracellular matrix. These results are compatible with the view that HSC function as liver specific pericytes participating in the regulation of sinusoidal blood pressure.
authors.affiliation: Research Group for Liver Study, INSERM E99 17, Victor Segalen University, Bordeaux, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Costa
authors.forename: B
authors.initials: B
authors.lastname: Tuchweber
authors.forename: L
authors.initials: L
authors.lastname: Rubbia-Brandt
authors.forename: S
authors.initials: S
authors.lastname: Peyrol
authors.forename: M
authors.initials: M
authors.lastname: Chevallie
authors.forename: M
authors.initials: M
authors.lastname: Adham
authors.forename: G
authors.initials: G
authors.lastname: Gabbiani
authors.forename: J
authors.initials: J
authors.lastname: Rosenbaum
authors.forename: A
authors.initials: A
authors.lastname: Desmoulière
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000818:Animals; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D018613:Microscopy, Confocal; D010477:Perfusion; D012333:RNA, Messenger / Q000378:metabolism; D013552:Swine
chemical_list: D000199:Actins; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger