title: Two forms of NAD-dependent D-mandelate dehydrogenase in Enterococcus faecalis IAM 10071.
abstract: Two forms of NAD-dependent D-mandelate dehydrogenase (D-ManDHs) were purified from Enterococcus faecalis IAM 10071. While these two enzymes consistently exhibited high activity toward large 2-ketoacid substrates that were branched at the C3 or C4 position, they gave distinctly different K(m) and V(max) values for these substrates and had distinct molecular weights by gel electrophoresis and gel filtration.
authors.affiliation: Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tamura
authors.forename: Atsuko
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Saho
authors.initials: S
authors.lastname: Iwai
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Wada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shinoda
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Arai
authors.forename: Shigeru
authors.initials: S
authors.lastname: Mineki
authors.forename: Mitsugi
authors.initials: M
authors.lastname: Iida
authors.forename: Hayao
authors.initials: H
authors.lastname: Taguchi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D007778:Lactobacillus / Q000201:enzymology; D013294:Lactococcus lactis / Q000201:enzymology; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism*
chemical_list: D009243:NAD; D000429:Alcohol Oxidoreductases; C043630:D-mandelate dehydrogenase