[The effect of early escheractomy on expression of ICAM-1 and E-selectin mRNA of endothelial cells].
OBJECTIVE
To examine the effect of burned serum on change in endothelial cell ICAM-1 and E-selectin mRNA expression and whether these alterations can be corrected by the means of escharectomy during burn shock period.


METHODS
One hundred and seventy-six Wistar rats with 30% III degree TBSA on back were studied. Endothelial cell ICAM-1 and E-selectin mRNA were tested with the method of RT-PCR.


RESULTS
ICAM-1 and E-selectin expression showed an increase after stimulation by serum of 4 hours post burn, reaching peak levels 168 hours after injury. ICAM-1 and E-selectin expression levels could decrease to baseline within 168 hours in the rats which underwent escharectomy during shock stage, but kept higher than normal in rats without escharectomy or underwent 96 hours post burn.


CONCLUSIONS
These findings suggest that eschar can induce endothelial cells to express adhesion molecule. Early escharectomy is important to prevent adhesion molecule expression and SIRS.
He
L
L
Burns Institute, 304th Hospital, People's Liberation Army, Beijing 100037.
Guo
Z
Z
Lu
Y
Y
Hao
D
D
Chai
J
J
Sheng
Z
Z
D000818:Animals; D002056:Burns / Q000097:blood* / Q000601:surgery; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis* / Q000235:genetics; D042783:Endothelial Cells / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000098:blood supply / Q000166:cytology; D008297:Male; D008833:Microcirculation / Q000166:cytology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D044967:Serum
D019040:E-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1