title: Induction of apoptosis in HL-60 cells by photochemically generated hydroxyl radicals.
abstract: Reactive oxygen species (ROS), especially hydroxyl radicals are postulated to mediate apoptosis of the cell. Here we demonstrate that hydroxyl radicals generated selectively by photolysis of a photo-Fenton reagent, N,N'-bis(2-hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthaldiimide (NP-III), induce apoptosis in HL-60 (human promyelocytic leukemia) cells involving the activation of caspase-3.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, 630-8506, Nara, Japan.
authors.forename: Sakiko
authors.initials: S
authors.lastname: Haruna
authors.forename: Rie
authors.initials: R
authors.lastname: Kuroi
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kajiwara
authors.forename: Ryoko
authors.initials: R
authors.lastname: Hashimoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Matsugo
authors.forename: Sadako
authors.initials: S
authors.lastname: Tokumaru
authors.forename: Shosuke
authors.initials: S
authors.lastname: Kojo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D017665:Hydroxyl Radical / Q000494:pharmacology* / Q000528:radiation effects; D009281:Naphthalenes; D010777:Photochemistry; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species / Q000494:pharmacology / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D009281:Naphthalenes; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; C067580:N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic diimide; D017665:Hydroxyl Radical; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases