title: Platelet release of transforming growth factor-beta and beta-thromboglobulin after in vitro contact with acrylic bone cements.
abstract: Three methacrylate-based bone cements used for the fixation of joint prostheses were evaluated: Sulfix-60 (Sulzer Orthopedic Inc., Baar, Switzerland). CMW1 (DePuy International Ltd., England). and CMW2 (DePuy International Ltd., England). The cements after polymerization were put in contact in vitro with platelet-rich plasma. Plasma, in contact only with siliconized glass, was used as a negative control. After contact, platelet number. beta-thromboglobulin (beta-TG), and transforming growth factor-beta1 (TGF-beta1) were determined. The Student's paired t test showed that the ccments induced no significant modifications of platelet number. CMWI and Sulfix-60 determined a significant increase in beta-TG compared with the negative control. All cements determined a significant increase in TGF-beta1. Significant differences were also seen in the levels of beta-TG and TGF-beta1 between cements with a content of benzoyl peroxide < 1 (Sulfix-60) and those with a content > 1 (CMW1 and CMW2). The cement with zirconium dioxide (Sulfix-60) produced higher levels of beta-TG and TGF-beta1, compared to those with barium sulphate (CMW1 and CMW2). In conclusion, all the cements induced the secretion of TGF-beta1 CMW1 and Sulfix-60 determined also a significant release of beta-TG. Platelet activation induced by the cements from one side could contribute to the pathogenesis of deep venous thrombosis, that often occurs after prosthetic implant and is caused also by other factors, including surgical trauma and venous stasis. From the other side, activated platelets can release growth factors favoring bone formation.
authors.affiliation: Dipartimento Putti, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: D
authors.initials: D
authors.lastname: Granchi
authors.forename: M
authors.initials: M
authors.lastname: Vancini
authors.forename: A
authors.initials: A
authors.lastname: Pizzoferrato
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology; D001466:Barium Sulfate / Q000737:chemistry / Q000494:pharmacology*; D001585:Benzoyl Peroxide / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D001843:Bone Cements / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008768:Methylmethacrylates / Q000737:chemistry / Q000494:pharmacology*; D010949:Plasma / Q000187:drug effects; D015539:Platelet Activation / Q000187:drug effects; D019904:Polymethyl Methacrylate; D013927:Thrombosis; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015040:Zirconium / Q000737:chemistry / Q000494:pharmacology*; D001620:beta-Thromboglobulin / Q000378:metabolism*
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D001843:Bone Cements; D008768:Methylmethacrylates; C460782:Sulfix-60; D016212:Transforming Growth Factor beta; D001620:beta-Thromboglobulin; D001466:Barium Sulfate; C035124:CMW cement; D019904:Polymethyl Methacrylate; D015040:Zirconium; D001585:Benzoyl Peroxide