Genetic identification of cinnamon (Cinnamomum spp.) based on the trnL-trnF chloroplast DNA.
Genetic identification among cinnamon species was studied by analyzing nucleotide sequences of chloroplast DNA from four species (Cinnamomum cassia, C. zeylanicum, C. burmannii and C. sieboldii). The two regions studied were the intergenic spacer region between the trnL 3'exon and trnF exon (trnL -trnF IGS) and the trnL intron region. We found nucleotide variation at one site in the trnL-trnF IGS, and at three sites in the trnL intron. With the sequence data from analysis of these regions, the four Cinnamomum species used in this study were correctly identified. Furthermore, single-strand conformation polymorphism (SSCP) analysis of PCR products from the trnL-trnF IGS and the trnL intron resulted in different SSCP band patterns among C. cassia, C. zeylanicum and C. burmannii.
Kojoma
Mareshige
M
Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Shizuoka, Japan. kojoma@postman.riken.go.
Kurihara
Kogo
K
Yamada
Kazuya
K
Sekita
Setsuko
S
Satake
Motoyoshi
M
Iida
Osamu
O
D001483:Base Sequence; D002935:Cinnamomum zeylanicum / Q000145:classification / Q000235:genetics*; D018742:DNA, Chloroplast / Q000737:chemistry / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012360:RNA, Transfer, Phe / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D012356:RNA, Transfer, Leu; D012360:RNA, Transfer, Phe