Correlation between in vitro and in vivo infectivity of Leishmania infantum clones.
Eleven clones of a single strain of Leishmania infantum (MCAN/ES/88/ISS441, Doba) were analyzed for biological behavior in vivo and in vitro. Different clones showed differences in growth dependent upon the two culture media employed. All clones displayed only slight differences in H2O2 and NaNO2 sensitivity compared to the original strain, whereas in vitro infectivity for mouse peritoneal macrophages differed significantly among the clones. In vivo infections in hamsters correlated strongly with in vitro infectivity. The phenotypic differences found suggest a polyclonal structure for the Leishmania infantum strain studied.
Méndez
S
S
National Institute of Allergy and Infectious Disease, The National Institutes of Health, Bethesda, Maryland 20892, USA.
Fernández-Pérez
F J
FJ
Santín
M
M
De La Fuente
C
C
Cuquerella
M
M
Gómez-Muñoz
M T
MT
Alunda
J M
JM
D000818:Animals; D002999:Clone Cells; D006224:Cricetinae; D003470:Culture Media; D004285:Dogs; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018314:Leishmania infantum / Q000254:growth & development* / Q000472:pathogenicity*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D017737:Macrophages, Peritoneal / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000494:pharmacology
D003470:Culture Media; D009569:Nitric Oxide; D006861:Hydrogen Peroxide