Expression of the histone H3 gene in the evaluation of cellular proliferation in colitis ulcerosa.
A comparison of the number of mRNA molecules of histone H3 in 1 microg total RNA extracted from colon sections sampled during colonoscopy was used to evaluate cellular proliferation activity in the rectum and sigmoid, in normal tissue and in colitis ulcerosa. Samples with similar intensity of the disease were selected for the study. Statistically significant differences between both groups of rectal sections were found in the expression of histone H3 encoding genes. The statistically significant result (p = 0.0485) indicates a more active division of cells in the healthy rectum, with no statistically significant differences in the sigmoid (p=0.9575).
Orchel
J
J
Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Katowice, Poland.
Mazurek
U
U
Bierzyńska-Macyszyn
G
G
Cholewa
K
K
Pastucha
E
E
Stadnicki
A
A
Wilczok
T
T
D015415:Biomarkers; D002455:Cell Division / Q000235:genetics; D003093:Colitis, Ulcerative / Q000473:pathology* / Q000503:physiopathology; D015870:Gene Expression; D006657:Histones / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000473:pathology; D016133:Polymerase Chain Reaction; D019914:Taq Polymerase
D015415:Biomarkers; D006657:Histones; D019914:Taq Polymerase