Isolation and expression of a novel neuron-specific onecut homeobox gene in zebrafish.
A complete cDNA of a novel zebrafish gene named onecut has been isolated; this gene encodes a protein of 446 amino acids with a Cut domain (73 amino acid residues) and a homeodomain. The Cut domain of zebrafish Onecut is highly similar to those in mammalian hepatocyte nuclear factor-6 and Drosophila Onecut, sharing 90 and 88% amino acid identity, respectively. The expression of zebrafish onecut is restricted to neuronal cells, being first detected in trigeminal ganglia neurons at the end of gastrulation. By the 1-somite stage, onecut expression has begun in primary neurons of the lateral stripes in the neural plate, and appeared in neuronal cells of the medial stripes at the 2-somite stage. By the 4-somite stage, onecut expression expanded to the intermediate stripes and to subsets of neuronal cells in the midbrain and hindbrain. Subsequently, onecut expression intensified in the lateral region of midbrain and hindbrain, yet no onecut-positive cells were seen in the telencephalon. By 24hpf, onecut transcripts remained abundant in the spinal cord but were no longer detectable in differentiated Rohon-Beard sensory neurons. The expression of onecut was greatly increased in the neural mutant mindbomb, while being decreased in narrowminded.
Hong
Sung-Kook
SK
Department of Genetic Engineering, Kyungpook National University, Daegu 702-701, South Korea.
Kim
Cheol-Hee
CH
Yoo
Kyeong-Won
KW
Kim
Hyung-Seok
HS
Kudoh
Tetsuhiro
T
Dawid
Igor B
IB
Huh
Tae-Lin
TL
D000818:Animals; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics*; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology; D013997:Time Factors; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
D018076:DNA, Complementary; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D029961:Zebrafish Proteins; C418486:onecut protein, Drosophila; C499617:onecut1 protein, zebrafish; C461685:onecutl protein, zebrafish