Differential staining of western blots of avian egg white glycoproteins using diverse lectins.
Avian egg white glycoproteins which differ in structure and carbohydrate composition, vary in their interactions with diverse lectins. Generally, wheat germ agglutinin (WGA) and concanavalin A (Con A) are used for the identification and separation of those of the chicken. In the present study, interactions of a battery of lectins, including: the above two, several galactophilic lectins (from Aplysia gonad (AGL), Erythrina corallodendron (ECorL), peanut (PNA) and Pseudomonas aeruginosa (PA-IL)), and fucose-binding lectins (from Ulex europaeus (UEA-I), Ulva lactuca (ULL) and P. aeruginosa (PA-IlL), which also binds mannose) with chicken, quail and pigeon egg white glycoproteins, were examined using both hemagglutination inhibition and Western blot analyses. The chicken egg white glycoproteins interacted most strongly with WGA, followed by Con A >> AGL = PA-IlL. The quail glycoprotein order of affinities was: Con A >> WGA = AGL = PA-IlL, while that of the pigeon was: AGL > PA-IL > WGA > Con A = PA-IlL. The blocking of the other lectins by the egg whites were insignificant. The results demonstrated the selectivity and efficiency of the five most reactive lectins for differential tagging of avian egg white glycoproteins and unveiled the profound heterogeneity of the latter, as well as the possible potential lectin usage for improving purification and quality control of the desired glycoproteins.
Lerrer
Batia
B
Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Gilboa-Garber
Nechama
N
D000818:Animals; D001717:Birds; D015153:Blotting, Western / Q000379:methods*; D004527:Egg Proteins / Q000032:analysis* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D037102:Lectins / Q000378:metabolism; D011485:Protein Binding; D013194:Staining and Labeling; D013379:Substrate Specificity
D004527:Egg Proteins; D006023:Glycoproteins; D037102:Lectins