[Single nucleotide polymorphism analysis in chicken insulin-like growth factor-II gene and its associations with growth and carcass traits].
In this experiment, F2 chicken derived from Broilers crossing with Silky was used to study the effect of insulin-like growth factor-II gene on growth and carcass traits. The partial gene was amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) was detected by the technique of restriction fragment length polymorphism (RFLP), and then confirmed by DNA sequencing. The mutation was found in the exon-2 of the gene, and can be clarified by cutting of restriction enzyme Aci-I. The result of least square analysis showed the gene was significantly related with growth and carcass traits. It implied that the insulin-like growth factor-II gene could be a genetic locus or linked to a major gene affecting greatly the growth and carcass traits in chicken.
Yan
Bing-Xue
BX
National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Li
Ning
N
Deng
Xue-Mei
XM
Hu
Xiao-Xiang
XX
Liu
Zhao-Liang
ZL
Zhao
Xing-Bo
XB
Lian
Zheng-Xing
ZX
Wu
Chang-Xin
CX
D000818:Animals; D002645:Chickens; D008040:Genetic Linkage; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000502:physiology; D016018:Least-Squares Analysis; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable
D007335:Insulin-Like Growth Factor II