[Construction of porous hydroxyapatite (HA) block loaded with cultured chondrocytes].
OBJECTIVE
To construct a kind of bone healing enhancing implant with cultured chondrocytes bound to hydroxyapatite (HA).


METHODS
Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm x 3 mm x 4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with alpha1 (II) cDNA probe.


RESULTS
Scanning electron micrographs showed that the pores of the HA surface and inside of the blocks are filled with cultured cells, especially the longer cultured block. The cells were chondrocytes confirmed by in situ hybridization.


CONCLUSIONS
The porous HA can be used as cell cultured substrate and chondrocyte can adhere and proliferate inside the porous HA block.
Yan
M
M
Department of Orthopaedic Surgery, Third Teaching Hospital, Beijing Medical University, Beijing 100083.
Dang
G
G
D000818:Animals; D002356:Cartilage / Q000166:cytology*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D017886:Durapatite; D008297:Male; D016348:Osseointegration / Q000502:physiology*; D051381:Rats
D017886:Durapatite