title: Effects of hypothermia on excitatory amino acids and metabolism in stroke patients: a microdialysis study.
abstract: OBJECTIVE
  The objective of this study was to assess the effect of therapeutic moderate hypothermia on excitatory amino acids and metabolism by applying cerebral microdialysis in patients suffering from space-occupying middle cerebral artery infarction.
  
  
  METHODS
  This was an open, prospective, observational study of 12 patients undergoing moderate hypothermia (33 degrees C) as rescue therapy for large, life-threatening middle cerebral artery infarction. Microdialysis probes were placed concomitantly with intracranial pressure (ICP) measuring devices in the frontal lobe of the infarcted and/or noninfarcted hemisphere. Using the CMA 600 Microdialysis Autoanalyzer, we analyzed glutamate, glycerol, pyruvate, and lactate.
  
  
  RESULTS
  According to follow-up cranial CT scans, 3 different compartments of microdialysis measurements could be defined. First, noninfarcted brain tissue had stable dialysate concentrations but a significant effect of hypothermia on glutamate (2.6 versus 3.6 micromol/L), lactate (1.8 versus 3 mmol/L), and pyruvate (50 versus 95.8 micromol/L). Second, measurements from peri-infarct tissue had a significant effect of hypothermia on glutamate (4.8 versus 12.6 micromol/L), glycerol (58 versus 82 micromol/L), lactate (0.7 versus 1.3 mmol/L), and pyruvate (13.3 versus 36.8 micromol/L). Third, dialysate concentrations obtained from irreversibly damaged tissue were excessive for glutamate (453 micromol/L), glycerol (1187 micromol/L), lactate (12 micromol/L), and pyruvate (4 micromol/L). In this extreme compartment, no effect of hypothermia was observed.
  
  
  CONCLUSIONS
  Cerebral microdialysis is a safe and feasible bedside method for neurochemical monitoring indicating normal brain tissue, potentially salvageable brain tissue, and irreversibly damaged areas in stroke. We could demonstrate that hypothermia decreases glutamate, glycerol, lactate, and pyruvate in the "tissue at risk" area of the infarct but not within the infarct core. Thus, future treatment strategies for life-threatening stroke should be guided by close neurochemical monitoring.
authors.affiliation: Department of Neurology, University of Heidelberg, Heidelberg, Germany. christian_berger@med.uni-heidelberg.de
authors.forename: Christian
authors.initials: C
authors.lastname: Berger
authors.forename: Wolf-Rüdiger
authors.initials: WR
authors.lastname: Schäbitz
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Georgiadis
authors.forename: Thorsten
authors.initials: T
authors.lastname: Steiner
authors.forename: Alfred
authors.initials: A
authors.lastname: Aschoff
authors.forename: Stefan
authors.initials: S
authors.lastname: Schwab
mesh_terms: D000328:Adult; D000368:Aged; D001921:Brain / Q000503:physiopathology; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007036:Hypothermia, Induced; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D007427:Intracranial Pressure; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D019289:Pyruvic Acid / Q000378:metabolism; D012044:Regression Analysis; D015996:Survival Rate; D014057:Tomography, X-Ray Computed
chemical_list: D019344:Lactic Acid; D018698:Glutamic Acid; D019289:Pyruvic Acid; D005990:Glycerol