[The feature of pancreatic microcirculatory impairment in caerulein induced acute pancreatitis].
OBJECTIVE
To study the feature of pancreatic microcirculatory impairment in caerulein-induced acute pancreatitis.


METHODS
The AP model of subcutaneous injection of caerulein was studied by intravital fluorescence microscopy of erythrocytes labeled by FITC (FITC-RBC) and by scanning electron microscopy of vascular corrosion casts and light microscopy of Chinese ink-injected/cleared tissues.


RESULTS
Animals treated with caerulein showed hyper-amylases. Contractions of intralobular arteriolar sphincter, presence of vacuoles in all the layers of sphincter, gross irregularity in capillary network of acini were observed in caerulein-induced groups. The decrease of pancreatic capillary blood flow (P < 0.01), reduction of functional capillary density, and irregular intermittent perfusion (P < 0.05) were found.


CONCLUSIONS
Impairment and contraction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis induced by Caerulein, and play a key role in the pancreatic ischemia and pancreatic microvascular failure in acute pancreatitis.
Zhou
Z
Z
Department of Hepato-Bili-Pancreato-Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041.
Zhang
Z
Z
Yan
L
L
Shu
Y
Y
Cheng
Z
Z
Zhao
J
J
Lan
P
P
Feng
X
X
Wang
R
R
D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D008297:Male; D008833:Microcirculation; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar
D002108:Ceruletide