title: Effects of 2-deoxy-D-glucose on DNA repair and mutagenesis in UV-irradiated yeast.
abstract: We have studied the effects of 2-deoxy-D-Glucose (2-DG) on the recovery of potentially lethal damage (PLDR), repair of chromosomal DNA, cyclobutane pyrimidine dimers (CPDs), reverse mutation and gene-conversion in UVC (254 nm) irradiated yeast. As studied by pulsed-field gel electrophoresis, post-irradiation chromosomal DNA repair kinetics in a phosphate buffer (PB) with 10 mM glucose (G) was biphasic, where the first phase exhibited a decrease and the second phase showed an increase in the band intensities. A post-irradiation treatment in PB + G (10 mM) with 2-DG (10, 20, 50 mM) reduced the decrease in the DNA band intensities in the first phase of DNA repair. As compared to a post-irradiation (125 J/m2) treatment in PB + G (10 mM), a treatment in PB + G (10 mM) + 2-DG (10 mM) showed a decreased PLDR, but increased revertants and gene-convertants.
authors.affiliation: Department of Molecular Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi-110054, India. balainma44@hotmail.com
authors.forename: M
authors.initials: M
authors.lastname: Bala
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: H C
authors.initials: HC
authors.lastname: Goel
mesh_terms: D002875:Chromosomes / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D003847:Deoxyglucose / Q000494:pharmacology*; D016296:Mutagenesis / Q000187:drug effects*; D012441:Saccharomyces cerevisiae; D014466:Ultraviolet Rays
chemical_list: D003847:Deoxyglucose