Screening of various glycosidases for the synthesis of octyl glucoside.
Thirteen glycosidases of microbial origin and almond beta-glycosidase were assayed in octanol/DMF (80:20, v/v), using a combination of hydrolysis, transglycosylation, and condensation reactions, in order to assess their potential for the production of alkyl glucosides. The two mesophile enzymes were highly impaired by the organic media. Three of the 11 thermophile enzymes gave interesting results in the hydrolysis and transglycosylation reactions, but they were highly inhibited by glucose. This made their use in a condensation reaction less interesting than the use of almond beta-glucosidase, which has a lower activity but shows less inhibition by the glucose.
Ducret
Amélie
A
Microbial and Enzyme Technology Group, Bioprocess Sector, Biotechnology Research Institute, National Research Council, Montreal, Quebec H4P 2R2 Canada.
Trani
Michael
M
Lortie
Robert
R
D000818:Animals; D001421:Bacteria, Anaerobic / Q000201:enzymology*; D002175:Candida / Q000201:enzymology*; D002417:Cattle; D005960:Glucosides / Q000032:analysis* / Q000096:biosynthesis*; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000378:metabolism; D006031:Glycosylation; D006868:Hydrolysis; D009754:Nuts / Q000201:enzymology*; D027861:Prunus; D013997:Time Factors
D005960:Glucosides; C018619:octyl-beta-D-glucoside; D006026:Glycoside Hydrolases