title: Physical and transcript map of the hereditary prostate cancer region at xq27.
abstract: We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an approximately 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at approximately 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
authors.affiliation: Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA. dstephan@cnmcresearch.org
authors.forename: Dietrich A
authors.initials: DA
authors.lastname: Stephan
authors.forename: Gareth R
authors.initials: GR
authors.lastname: Howell
authors.forename: Tanya M
authors.initials: TM
authors.lastname: Teslovich
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Coffey
authors.forename: Lorie
authors.initials: L
authors.lastname: Smith
authors.forename: Joan E
authors.initials: JE
authors.lastname: Bailey-Wilson
authors.forename: Lindsay
authors.initials: L
authors.lastname: Malechek
authors.forename: Derek
authors.initials: D
authors.lastname: Gildea
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Smith
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Gillanders
authors.forename: Johanna
authors.initials: J
authors.lastname: Schleutker
authors.forename: Ping
authors.initials: P
authors.lastname: Hu
authors.forename: Helen E
authors.initials: HE
authors.lastname: Steingruber
authors.forename: Pawandeep
authors.initials: P
authors.lastname: Dhami
authors.forename: Christiane M
authors.initials: CM
authors.lastname: Robbins
authors.forename: Izabela
authors.initials: I
authors.lastname: Makalowska
authors.forename: John D
authors.initials: JD
authors.lastname: Carpten
authors.forename: Raman
authors.initials: R
authors.lastname: Sood
authors.forename: Steve
authors.initials: S
authors.lastname: Mumm
authors.forename: Rolland
authors.initials: R
authors.lastname: Reinbold
authors.forename: Tom I
authors.initials: TI
authors.lastname: Bonner
authors.forename: Agnes
authors.initials: A
authors.lastname: Baffoe-Bonnie
authors.forename: Lukas
authors.initials: L
authors.lastname: Bubendorf
authors.forename: Mervi
authors.initials: M
authors.lastname: Heiskanen
authors.forename: Olli P
authors.initials: OP
authors.lastname: Kallioneimi
authors.forename: Andreas D
authors.initials: AD
authors.lastname: Baxevanis
authors.forename: Shirin S
authors.initials: SS
authors.lastname: Joseph
authors.forename: Ileana
authors.initials: I
authors.lastname: Zucchi
authors.forename: Robert D
authors.initials: RD
authors.lastname: Burk
authors.forename: William
authors.initials: W
authors.lastname: Isaacs
authors.forename: Mark T
authors.initials: MT
authors.lastname: Ross
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Trent
mesh_terms: D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D027042:Chromosomes, Artificial, P1 Bacteriophage / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000209:etiology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014960:X Chromosome / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D012333:RNA, Messenger