Discovering the sweeping power of point mutations using a GIRAFF.
In pathogenic bacteria, point and other simple mutations can provide a strong selective advantage during the course of a single infection. Our understanding of the importance of these randomly occurring mutations has been hampered by a lack of technologies allowing mutation scanning on a genomic scale. Here, a novel technology is described that makes it possible to scan, in a single Southern blot experiment, the sequence identity of genomic regions with a combined length of hundreds of kilobases.
Sokurenko
E V
EV
Dept of Microbiology, University of Washington, Seattle 98195, USA. evs@u.washington.edu
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis; D017354:Point Mutation; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods
D009692:Nucleic Acid Heteroduplexes