hKAP1.6 and hKAP1.7, two novel human high sulfur keratin-associated proteins are expressed in the hair follicle cortex.
Hair fiber differentiation involves the expression of both hair keratin intermediate filament proteins and their associated proteins, termed keratin-associated proteins. In this study, cDNA clones encoding two novel keratin-associated proteins were isolated from human hair follicle mRNA. The predicted amino acid sequence derived from these clones revealed that these proteins represent members of the human keratin-associated protein 1 family. They show strong sequence homology to two previously described keratin-associated protein 1 family members hKAP1.1 A and hKAP1.1B. We have called these new proteins hKAP1.6 and hKAP1.7, respectively. RNA in situ hybridization studies of human anagen hair follicles using a conserved probe for these four keratin-associated protein 1 members demonstrated the expression of this group in the differentiated portions of the hair cortex.
Shimomura
Yutaka
Y
Department of Dermatology, Niigata University School of Medicine, Asahimachi-dori, Niigata, Japan.
Aoki
Noriaki
N
Rogers
Michael A
MA
Langbein
Lutz
L
Schweizer
Jürgen
J
Ito
Masaaki
M
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000302:isolation & purification; D018859:Hair Follicle / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007633:Keratins / Q000235:genetics / Q000378:metabolism*; D053564:Keratins, Hair-Specific; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013455:Sulfur / Q000378:metabolism*; D014018:Tissue Distribution
D018076:DNA, Complementary; C450379:KRTAP1-1 protein, human; D053564:Keratins, Hair-Specific; D007633:Keratins; D013455:Sulfur