14-3-3 Protein isoforms and atypical patterns of the 14-3-3 assay in the diagnosis of Creutzfeldt-Jakob disease.
A positive 14-3-3 assay is a criterion for probable Creutzfeldt-Jakob disease (CJD). Cerebrospinal fluid (CSF) 14-3-3 is usually detected by immunoblot using an antibody that recognizes all of the 14-3-3 isoforms. In a few cases, the antibody recognizes an inferior band and this pattern is associated with false positive results. We analyzed 43 CSF (26 CJD, 17 controls) samples using antibodies against specific isoforms (beta, epsilon, gamma, tau, xi) and compared the results with those obtained with the standard antibody. The anti-gamma and anti-beta antibody achieved similar results but the presence of atypical patterns made the standard antibody more accurate for the CJD diagnosis. To study the nature of the inferior band, CSF samples were probed with antibodies against light chain immunoglobulins, and immunoblots of human IgG with the standard antibody. The experiments suggested a cross-reaction of the anti-14-3-3 antibody with light chain immunoglobulins.
Sánchez-Valle
Raquel
R
Unidad de Biodiagnóstico de la Enfermedad de Creutzfeldt-Jakob, Service of Neurology, Institut d'Investigació Biomèdica August Pi i Sunyer (IDIBAPS), Hospital Clinic, University of Barcelona, Villarroel 170, 08036 Barcelona, Spain.
Saiz
Albert
A
Graus
Francesc
F
D048948:14-3-3 Proteins; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid*; D003429:Cross Reactions; D005189:False Positive Reactions; D006801:Humans; D015151:Immunoblotting; D007147:Immunoglobulin Light Chains / Q000134:cerebrospinal fluid*; D020033:Protein Isoforms / Q000134:cerebrospinal fluid*; D014446:Tyrosine 3-Monooxygenase / Q000134:cerebrospinal fluid*
D048948:14-3-3 Proteins; D007147:Immunoglobulin Light Chains; D020033:Protein Isoforms; D014446:Tyrosine 3-Monooxygenase