Engineering protein and cell adhesivity using PEO-terminated triblock polymers.
Previous studies on customizing cell culture environments have utilized a variety of microfabrication-based tools to control the spatial localization of adhesive proteins and subsequently mammalian cells. Others have used various methods to immobilize nonadhesive PEO-based polymers on surfaces to inhibit protein absorption and cell adhesion. In this study, we report the application of a well-characterized, commercially available, PEO-terminated triblock polymer (Pluronic F108) to create micropatterned nonadhesive domains on a variety of biomaterials that deter cell adhesion for up to 4 weeks in culture. The Pluronic can be applied using microfluidic tools or photolithographic techniques, and can be adsorbed to a variety of common surfaces including tissue culture polystyrene, methylated glass, silicone, and polylactic-co-glycolic acid. The effectiveness of the Pluronic in inhibiting cell adhesion in the presence of collagen I is also quantified. Finally, these patterning techniques are generalized to control tissue organization on a variety of common biomaterials. This simple method for micropatterning PEO and, therefore, proteins and cells should prove useful as a tool for biomolecular surface engineering.
Liu
Valerie A
VA
Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.
Jastromb
William E
WE
Bhatia
Sangeeta N
SN
D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008854:Microscopy, Electron; D036103:Nanotechnology / Q000295:instrumentation*; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D015202:Protein Engineering; D013499:Surface Properties
D016326:Extracellular Matrix Proteins; D020442:Poloxamer; D011092:Polyethylene Glycols