Two antioxidative lactobacilli strains as promising probiotics.
Two antioxidative strains tentatively identified as Lactobacillus fermentum, E-3 and E-18, were isolated from intestinal microflora of a healthy child. Survival time of these strains in the presence of reactive oxygen species (ROS), like hydrogen peroxide, superoxide anions and hydroxyl radicals, was significantly increased compared with a non-antioxidative strain, and also was quite similar to a highly ROS resistant strain of Salmonella typhimurium. E-3 and E-8 contain a remarkable level of glutathione, express Mn-SOD, which is important for the prevention of lipid peroxidation, and secrete hydrogen peroxide. Their significant antimicrobial activity combined with antioxidative properties may serve as defensive principles in the intestinal microbial ecosystem and overcome exo- and endogenous oxidative stress.
Kullisaar
Tiiu
T
Department of Biochemistry, Medical Faculty, University of Tartu, Estonia. tiiukul@ut.ee
Zilmer
Mihkel
M
Mikelsaar
Marika
M
Vihalemm
Tiiu
T
Annuk
Heidi
H
Kairane
Ceslava
C
Kilk
Ann
A
D002648:Child; D004064:Digestive System / Q000382:microbiology; D005516:Food Microbiology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007223:Infant; D007778:Lactobacillus / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000502:physiology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D019936:Probiotics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D005978:Glutathione