Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation.
After radiofrequency ablation (RFA), hepatocellular carcinoma undergoes complete necrosis and an ongoing necrosis that is irreversible and characterized histologically by disrupted cell outlines, homogenous cytoplasmic eosinophilia, and preserved nuclear staining, with the cells appearing quite distinct from viable cancer cells. Antibody to detect single-stranded DNA (ssDNA) specifically labeled nuclei in the setting of ongoing necrosis, but not viable tumor cells, whereas human mitochondrial antibody labeled the cytoplasm of viable cells but not cells of ongoing necrosis. The results demonstrate that RFA causes denaturation of both DNA and proteins and that the immunohistochemistry of ssDNA and mitochondrial protein is useful in detection of ongoing necrosis after RFA and provides pathological information on the validity of this procedure.
Itoh
Tomoo
T
Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Hokkaido, Japan.
Orba
Yasuko
Y
Takei
Hidehiro
H
Ishida
Yusuke
Y
Saitoh
Makoto
M
Nakamura
Hideaki
H
Meguro
Takashi
T
Horita
Shoichi
S
Fujita
Miri
M
Nagashima
Kazuo
K
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D017115:Catheter Ablation; D002467:Cell Nucleus / Q000737:chemistry / Q000473:pathology; D004277:DNA, Single-Stranded / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry / Q000473:pathology; D009336:Necrosis
D004277:DNA, Single-Stranded