title: Comparative study of the high molecular mass fraction and low molecular mass fraction of Sho-saiko-to in a murine immunologically induced liver injury model.
abstract: We compared the pharmacological actions of the high and low molecular mass fractions of Sho-saiko-to using a murine immunologically induced liver injury model to estimate the roles of these fractions in the expression of the pharmacological action. In a Bacillus Calmette-Guerin (BCG)/lipopolysaccharide (LPS)-induced liver injury model, Sho-saiko-to and both of its fractions significantly reduced the increases in the aminotranseferase levels in serum. They also reduced the increase in the nitric oxide (NOx) level in serum. On the other hand, Sho-saiko-to and its high molecular mass fraction suppressed the increase in plasma NOx level in an LPS-induced endotoxin shock model but its low molecular mass fraction did not. These results suggest the possibility that both fractions act hepatoprotectively in a different manner. We believe that these results can help to elucidate the mechanism of action of ingredients in Sho-saiko-to.
authors.affiliation: Laboratory of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan. nose@phar.nagoya-cu.ac.jp
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Nose
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Terawaki
authors.forename: Naomi
authors.initials: N
authors.lastname: Iwahashi
authors.forename: Kanayo
authors.initials: K
authors.lastname: Oguri
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ogihara
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009569:Nitric Oxide / Q000097:blood; D012772:Shock, Septic / Q000139:chemically induced / Q000188:drug therapy
chemical_list: D001500:BCG Vaccine; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D008070:Lipopolysaccharides; D009569:Nitric Oxide; C038044:baicalin; C018142:saiko-keishi-to; D019695:Glycyrrhizic Acid