Inhibition of chlorogenic acid-induced cytotoxicity by CoCl2.
Chlorogenic acid (CGA) induced apoptotic cell death in human oral squamous cell carcinoma (HSC-2) and salivary gland tumor (HSG) cell lines. CGA exhibited oxidation potential in the culture medium, as demonstrated by NO monitor. Both cytotoxic activity and oxidation potential were significantly reduced by the addition of CoCl2. ESR spectroscopy showed that CGA produced seven peaks of radicals under alkaline condition, while addition of CoCl2 altered the spectral pattern and diminished the radical intensity of CGA. CoCl2 accelerated the CGA-induced coloration of the culture medium and modified the difference spectrum at around 325 nm, an absorption maximum characteristic of CGA. These data suggest that CoCl2 induced conformational changes in the CGA molecule.
Jiang
Y
Y
Department of Dental Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan.
Satoh
K
K
Watanabe
S
S
Kusama
K
K
Sakagami
H
H
D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D002726:Chlorogenic Acid / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology*; D004347:Drug Interactions; D004578:Electron Spin Resonance Spectroscopy; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000494:pharmacology; D012468:Salivary Gland Neoplasms / Q000188:drug therapy; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D016877:Oxidants; D002726:Chlorogenic Acid; D003035:Cobalt; C018021:cobaltous chloride