Analysis of the p53 gene and papillomavirus detection in smears from cervical lesions.
BACKGROUND
Alterations of the p53 tumor suppressor gene are correlated with a critical step in the development of many human cancers. The tumor suppressor gene functions include regulation of the cell cycle and the cellular response to DNA damage, initiation of DNA repair and replication, induction of apoptosis and promotion of cell differentiation.


METHODS
Smears from ten cases of cervical lesions were analyzed for status of exons 5-8 of the p53 gene using PCR/SSCP. HPV infection was also screened by the PCR method using two PCR primer sets. Changes in the p53 gene were observed in a case of squamous carcinoma and a case of asymptomatic cervical intraepithelial neoplasia grade III (CIN III). High-risk HPV was detected in both cases showing that HPV infection and p53 mutation are not exclusive events.
dos Santos Oliveira
Ledy do Horto
Ldo H
Department of Microbiology and Parasitology, Biomedical Institute, Universidade Federal Fluminense, Rio de Janeiro, Brazil. mipledy@centrain.com.br
Fernandez
André de Paula
Ade P
Xavier
Brunno Lessa Saldanha
BL
Machado Rodrigues
Eliana de Vasconcelos
Ede V
Cavalcanti
Silvia Maria Baeta
SM
D005091:Exons / Q000235:genetics; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications* / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014412:Tumor Virus Infections / Q000150:complications* / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000821:virology*; D014626:Vaginal Smears