Sol-gel encapsulation of lactate dehydrogenase for optical sensing of L-lactate.
Sol-gel encapsulation of lactate dehydrogenase and its cofactor can be employed as a disposable sensor for L-lactate. The sensor utilized the changes in absorbance or fluorescence from the reduced cofactor nicotinamide adenine dinucleotide (NADH) upon exposure to L-lactate. Although, problems such as diminished enzymatic activity and/or leaching of enzyme from the sol-gel matrix occurred, the sol-gel process is sufficiently mild to permit retention of enzymatic activity. The apparent activity of LDH in the sensor is at least 10% of that of the dissolved enzyme. The sensor has a linear dynamic range over the normal physiological L-lactate level and has a long-term storage stability of at least 3 weeks.
Li
Chia I
CI
Department of Chemistry, National Chung Cheng University, Ming-Hsiung, 621, Chia-Yi, Taiwan, ROC.
Lin
Yi Hua
YH
Shih
Cheng Ling
CL
Tsaur
Jeng Pyng
JP
Chau
Lai Kwan
LK
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D017640:Silicates / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D004800:Enzymes, Immobilized; D005782:Gels; D017640:Silicates; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase