Evaluation of a system producing the hemopoietic factor. WEHI-3B cell line function, when encapsulated in a polypropylene hollow fibre.
The purpose of our study was evaluation of functioning of WEHI-3B (an mouse cell line producing IL-3) cells encapsulated in hollow fibers (HF). In vitro: the WEHI-3B cells were encapsulated in HF of polypropylene K600 silikonized, and cultured over two weeks. In vivo: the encapsulated WEHI-3B after weeks culture, were implanted subcutaneously into mice for 1 week. After explantation encapsulated WEHI 3-B were cultured again in culture medium for one week. The production of IL-3 by encapsulated WEHI-3B cells was assessed by evaluation of IL-3 dependent, BaF3 cells viability. The percent number of one day survival of BaF3 cells in the culture medium supplemented with 15% of encapsulated WEHI-3B in vitro or encapsulated WEHI-3B after in vivo conditioned medium was comparable with positive control. Possible replacement of recombinant cytokines with HF encapsulated cytokine-producing cells may be a chance for continous supplementation of the factors for hematopoietic stem cells differentiation.
Granicka
L H
LH
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw.
Hoser
G
G
Weryński
A
A
Kawiak
J
J
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002470:Cell Survival; D017077:Culture Media, Conditioned / Q000378:metabolism; D006410:Hematopoiesis; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000378:metabolism; D015479:Leukemia, Myelomonocytic, Acute; D051379:Mice; D011126:Polypropylenes; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism
D017077:Culture Media, Conditioned; D007377:Interleukin-3; D011126:Polypropylenes