title: [Bone scintigraphy in evaluating revascularization of the heat-devitalised autografts in adult dogs].
abstract: OBJECTIVE
  To establish an animal model of microwave hyperthermic devitalization of long bones and compare bone scintigraphy of the devitalised bone with pathologic examination.
  
  
  METHODS
  16 Mongrel adult dogs were used. Enght 8 cm of one femur of each dog was exposed and isolated by heat-proof material. Two microwave antennas were inserted into the medullary cavity at a distance of 2.5 cm, and the bone was heated intermittently to maintain a surface temperature of 50 degrees C to 60 degrees C for 30 minutes. Bone scintigraphy was carried out at different postoperative time, combined with tetracycline labeling, microangiography with Indian ink and HE staining.
  
  
  RESULTS
  About 5 cm - 6 cm of segmental femur was deprived of blood supply after devitalization which was confirmed by microangiography and negative labeling of tetracyclines. At two weeks, the devitalised bone demonstrated a cold region on the scintiscan and no revascularization in microangiography. 12 - 16 weeks after the operation, partial devitalised bone was revascularized and HE staining showed widel bone resorption and minimum formation of new bone. However, the radionuclide uptakes of the devitalised segments were greater than those of the normal femurs. At one year, the devitalised segment was well (not completely) revascularised and showed evident appositional new bone formation. The scintigraphic bone-imaging was slightly lower than that of the normal femur.
  
  
  CONCLUSIONS
  A strong positive scintiscan in early stage might demonstrate a good potential of revascularization of the heat-devitalised cortical bone rather than its viability. And the scintiscans of long-term follow-up more than one year should be more informative.
authors.affiliation: Department of Orthopaedics, Second Affiliated Hospital, Fourth Military Medical University, Xi'an 710038.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Fan
authors.forename: B
authors.initials: B
authors.lastname: Ma
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: X
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000792:Angiography / Q000379:methods; D000818:Animals; D004285:Dogs; D005269:Femur / Q000098:blood supply* / Q000000981:diagnostic imaging* / Q000601:surgery; D006358:Hot Temperature; D008297:Male; D011877:Radionuclide Imaging; D013997:Time Factors