title: Evolution of the regulators of G-protein signaling multigene family in mouse and human.
abstract: The regulators of G-protein signaling (RGS) proteins are important regulatory and structural components of G-protein coupled receptor complexes. RGS proteins are GTPase activating proteins (GAPs) of Gi-and Gq-class Galpha proteins, and thereby accelerate signaling kinetics and termination. Here, we mapped the chromosomal positions of all 21 Rgs genes in mouse, and determined human RGS gene structures using genomic sequence from partially assembled bacterial artificial chromosomes (BACs) and Celera fragments. In mice and humans, 18 of 21 RGS genes are either tandemly duplicated or tightly linked to genes encoding other components of G-protein signaling pathways, including Galpha, Ggamma, receptors (GPCR), and receptor kinases (GPRK). A phylogenetic tree revealed seven RGS gene subfamilies in the yeast and metazoan genomes that have been sequenced. We propose that similar systematic analyses of all multigene families from human and other mammalian genomes will help complete the assembly and annotation of the human genome sequence.
authors.affiliation: Pharmacology Department, UT Southwestern, Dallas, Texas 75390-9041, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Sierra
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: Deborah
authors.initials: D
authors.lastname: Householder
authors.forename: Nick V
authors.initials: NV
authors.lastname: Grishin
authors.forename: Kan
authors.initials: K
authors.lastname: Yu
authors.forename: Pallavi
authors.initials: P
authors.lastname: Ukidwe
authors.forename: Sheryll A
authors.initials: SA
authors.lastname: Barker
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Theodore G
authors.initials: TG
authors.lastname: Wensel
authors.forename: Glen
authors.initials: G
authors.lastname: Otero
authors.forename: Greg
authors.initials: G
authors.lastname: Brown
authors.forename: Neal G
authors.initials: NG
authors.lastname: Copeland
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Wilkie
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D005810:Multigene Family; D010802:Phylogeny; D020710:RGS Proteins / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D020710:RGS Proteins