Priming and activation of mouse macrophages by trehalose 6,6'-dicorynomycolate vesicles from Corynebacterium glutamicum.
Vesicles consisting of pure trehalose dicorynomycolate (TDCM), the corynebacterial analog of the most studied mycobacterial glycolipid 'cord factor', were isolated from Corynebacterium glutamicum cells by mild detergent treatment; these induced in vivo a macrophage priming similar to that obtained with mycobacterial-derived trehalose dimycolate. In vitro, both TDCM and bacterial lipopolysaccharide (LPS) induced in macrophages the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), endotoxin tolerance, and were primed for an enhanced secondary NO response to LPS. Interferon-gamma pretreatment did not influence the LPS-induced TNF-alpha response, but considerably increased the TDCM-induced response.
Chami
Mohamed
M
Laboratoire des Biomembranes, Institute de Biophysique et Biochimie Moléculaire et cellulaire, Centre de La Recherche Scientifique, Orsay, France.
Andréau
Karine
K
Lemassu
Anne
A
Petit
Jean-François
JF
Houssin
Christine
C
Puech
Virginie
V
Bayan
Nicolas
N
Chaby
Richard
R
Daffé
Mamadou
M
D000276:Adjuvants, Immunologic; D000818:Animals; D002478:Cells, Cultured; D003311:Cord Factors / Q000276:immunology* / Q000302:isolation & purification; D003352:Corynebacterium / Q000276:immunology*; D005260:Female; D008262:Macrophage Activation / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology*; D051379:Mice
D000276:Adjuvants, Immunologic; D003311:Cord Factors; C043399:6,6'-dicorynomycolyl trehalose