Reduction of marginal zone B cells in CD22-deficient mice.
CD22 is a B cell-specific member of the immunoglobulin superfamily and binds to sialic acid. CD22 inhibits B cell receptor signaling. Mice deficient for CD22 show a largely normal B cell development. Here, we have performed a detailed analysis of the splenic B cell population and found that the subset of marginal zone (MZ) B cells was selectively reduced in CD22-deficient mice. CD22-deficient mice showed a lack of TNP-ficoll capturing cells in the MZ and a reduced response to TNP-ficoll, particularly when the antigen was applied intravenously. CD22-deficient B cells showed both enhanced motility as well as enhanced chemotaxis to certain chemokines. The altered chemokine responsiveness or the higher signaling capacity of CD22-deficient B cells may lead to the compromised MZ B cell compartment, as both processes have previously been shown to affect MZ composition.
Samardzic
Tatjana
T
Department of Physiological Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
Marinkovic
Dragan
D
Danzer
Claus-Peter
CP
Gerlach
Judith
J
Nitschke
Lars
L
Wirth
Thomas
T
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics / Q000378:metabolism*; D000955:Antigens, T-Independent / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D015815:Cell Adhesion Molecules; D002465:Cell Movement / Q000276:immunology; D005362:Ficoll / Q000031:analogs & derivatives*; D037102:Lectins; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051918:Sialic Acid Binding Ig-like Lectin 2; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014301:Trinitrobenzenes
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000955:Antigens, T-Independent; C497868:Cd22 protein, mouse; D015815:Cell Adhesion Molecules; D037102:Lectins; D051918:Sialic Acid Binding Ig-like Lectin 2; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll