A hydroxysteroid sulfotransferase, st2b2, is a skin cholesterol sulfotransferase in mice.
The mRNA of a sulfotransferase (St2b2) mediating cholesterol sulfation was detected in mouse skin. Recombinant St2b2 also mediated the sulfation of pregnenolone, 3beta-hydroxy-5-cholen-24-oic acid, and dehydroepiandrosterone. St2b2 protein was detected in skin cytosols on Western blotting. The addition of 10 nM TPA to skin epidermal cells from newborn mice resulted in a twofold increase in cholesterol sulfation and concomitantly enhanced the St2b2 content after 40 h. Other candidate cholesterol sulfotransferases, St2a4 and St2a9, were not detected in skin by RT-PCR. These results indicate that St2b2 is a cholesterol sulfotransferase in mouse skin.
Shimada
Miki
M
Division of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980-8578, Japan. shimada@mail.pharm.tohoku.ac.jp
Kamiyama
Yoshiteru
Y
Sato
Akira
A
Honma
Wataru
W
Nagata
Kiyoshi
K
Yamazoe
Yasushi
Y
D000276:Adjuvants, Immunologic / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002784:Cholesterol / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D003687:Dehydroepiandrosterone / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D011284:Pregnenolone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D015238:Sulfotransferases / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D017931:DNA Primers; D012333:RNA, Messenger; D003687:Dehydroepiandrosterone; D013455:Sulfur; D011284:Pregnenolone; D002784:Cholesterol; C534506:St2b2 protein, mouse; D015238:Sulfotransferases; C043456:cholesterol sulfotransferase; C028152:alcohol sulfotransferase; D013755:Tetradecanoylphorbol Acetate