title: Identification of a satellite double-stranded RNA in the parasitic protozoan Trichomonas vaginalis infected with T. vaginalis virus T1.
abstract: Co-infection by a 0.5-kb small double-stranded (ds) RNA together with Trichomonas vaginalis virus (TVV) genomic 4.6-kb dsRNA is commonly observed in a number of T. vaginalis isolates. By molecular cloning and primer extension experiments, the 497-bp cDNA sequence of a 0.5-kb dsRNA co-infecting with TVV-T1 in T vagina/is T1 isolate was elucidated. Consistent with the replication cycle of a typical dsRNA virus, a plus-strand viral RNA beginning at +1 of the 0.5-kb dsRNA was identified in infected T. vaginalis T1 cells by primer extension and Northern hybridization studies. The 0.5-kb dsRNA was separately encased in TVV capsids from the viral genomic dsRNA, as shown by protein analysis and electron microscopic examination of viral particles purified by multiple rounds of CsCl gradient centrifugation. The riboprobes transcribed from a cloned cDNA of the 0.5-kb dsRNA exhibited strong hybridization to a small dsRNA in a T vaginalis T9 isolate, which harbors a TVV-T9 distantly related to TVV-T1, but the same probes showed very little hybridization to the viral genomic dsRNA of both TVV-T1 and TVV-T9. Very little sequence homology between the 0.5-kb dsRNA and the 4.6-kb dsRNA in TVV-T1 was found by computer-assisted analysis, suggesting that the small dsRNA in T. vaginalis T1 is not derived from the genome of TVV-T1 or other distantly related T. vaginalis viruses. These results suggest that the small dsRNAs in T vaginalis are satellite RNAs of T. vaginalis virus.
authors.affiliation: Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
authors.forename: J H
authors.initials: JH
authors.lastname: Tai
authors.forename: S C
authors.initials: SC
authors.lastname: Chang
authors.forename: C F
authors.initials: CF
authors.lastname: Ip
authors.forename: S J
authors.initials: SJ
authors.lastname: Ong
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D012328:RNA Viruses / Q000235:genetics* / Q000502:physiology; D012330:RNA, Double-Stranded / Q000235:genetics*; D019244:RNA, Satellite / Q000235:genetics*; D016415:Sequence Alignment; D014246:Trichomonas vaginalis / Q000821:virology*; D014779:Virus Replication / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D019244:RNA, Satellite