Study on the mechanism of regulation on the peritoneal lymphatic stomata with Chinese herbal medicine.
OBJECTIVE
To study the mechanism of Chinese herbal medicine (CHM), the prescription consists of Radix Salviae Miltiorrhizae, Radix Codonopsitis Pilosulae, Rhizoma Atractylodis Alba and Rhizoma Alismatis, Leonurus Heterophyllus Sweet,etc on the regulation of the peritoneal lymphatic stomata and the ascites drainage.


METHODS
The mouse model of live fibrosis was established with the application of intragastric installations of carbon tetrachloride once every three days; scanning electron microscope and computer image processing were used to detect the area and the distributive density of the peritoneal lymphatic stomata; and the concentrations and NO in the serum were measured and analyzed in the experiment.


RESULTS
Two different doses of CHM could significantly increase the area of the peritoneal lymphatic stomata, promote its distributive density and enhance the drainage of urinary ion such as sodium, potassium and chlorine. Meanwhile, the NO concentration of two different doses of CHM groups was 133.52+/-23.57 micromol/L and 137.2+/-26.79 micromol/L respectively. In comparison with the control group and model groups (48.36+/-6.83 micromol/L and 35.22+/-8.94 micromol/L, P<0.01),there existed significantly marked difference, this made it clear that Chinese herbal medicine could induce high endogenous NO concentration. The effect of Chinese herbal medicine on the peritoneal lymphatic stomata and the drainage of urinary ion was altered by adding NO donor(sodium nitropurruside,SNP) or NO synthase (NOS) inhibitor (N(G)-monomethyl-L-arginine, L-NMMA) to the peritoneal cavity.


CONCLUSIONS
There existed correlations between high NO concentration and enlargement of the peritoneal lymphatic stomata, which result in enhanced drainage of ascites. These data supported the hypothesis that Chinese herbal medicine could regulate the peritoneal lymphatic stomata by accelerating the synthesis and release of endogenous NO.
Ding
Shi-Ping
SP
Department of Lymphology, Department of Histology and Embryology, Medical College of Zhejiang University School of Medicine, Hangzhou 310031, China.
Li
Ji-Cheng
JC
Xu
Jian
J
Mao
Lian-Gen
LG
D000818:Animals; D001201:Ascites / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002712:Chlorides / Q000652:urine; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008208:Lymphatic System / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009569:Nitric Oxide / Q000097:blood; D010537:Peritoneum / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000652:urine; D012964:Sodium / Q000652:urine
D002712:Chlorides; D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide; D012964:Sodium; D011188:Potassium