title: [Molecular and genetic polymorphism in three cattle breeds].
abstract: Determination of cattle breed and assignment of individual animals to that or another group based on distinctions of genotype is of great importance in genetics and selection. Using of PCR-analysis opened new possibilities for typing DNA. The procedure of PCR was optimized for animal DNA. Two methods of PCR, RAPD and SSRP, were applied for investigation of molecular-genetical polymorphism in three cattle breeds (Ukrainian red-motley lactescent, Golshtine and Simmental). A dendrogram reflecting genetical mutual relations between analyzed animals was drawn. The level of homo-heterozygosity and the distribution of microsatellite alleles were determined. System for investigation of molecular-genetical polymorphism in the cattle breeds and characterization of genotype of individual animals were developed and tested on a real population.
authors.forename: M V
authors.initials: MV
authors.lastname: Zubets
authors.forename: V P
authors.initials: VP
authors.lastname: Burkat
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Sivolap
authors.forename: V E
authors.initials: VE
authors.lastname: Kuznetsov
authors.forename: I N
authors.initials: IN
authors.lastname: Lovenchuk
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D013045:Species Specificity; D014455:Ukraine
chemical_list: D004247:DNA