Weight loss-induced rise in plasma pollutant is associated with reduced skeletal muscle oxidative capacity.
In this study, we examined whether weight loss-induced changes in plasma organochlorine compounds (OC) were associated with those in skeletal muscle markers of glycolytic and oxidative metabolism. Vastus lateralis skeletal muscle enzyme activities and plasma OC (Aroclor 1260, polychlorinated biphenyl 153, p,p'-DDE, beta-hexachlorocyclohexane, and hexachlorobenzene) were measured before and after a weight loss program in 17 men and 20 women. Both sexes showed a similar reduction in body weight (approximately 11 kg) in response to treatment, although men lost significantly more fat mass than women (P < 0.05). Enzymatic markers of glycolysis, phosphofructokinase (PFK) activity, and oxidative metabolism, beta-hydroxyacyl-CoA dehydrogenase (HADH), citrate synthase (CS), and cytochrome c oxidase (COX) activities, remained unchanged after weight loss. A significant increase in plasma OC levels was observed in response to weight loss, an effect that was more pronounced in men. No relationship was observed between changes in OC and those in PFK activity in either sex [-0.31 < r < 0.12, not significant (NS)]. However, the greater the increase in plasma OC levels, the greater the reduction in oxidative enzyme (HADH, CS, COX) activities was in response to weight loss in men (-0.75 < r < -0.50, P < 0.05) but not in women (-0.33 < r < 0.33, NS). These results suggest that the weight loss-induced increase in plasma pollutant levels is likely to be associated with reduced skeletal muscle oxidative metabolism in men but not in women.
Imbeault
Pascal
P
Department of Social and Preventive Medicine, Laval University, Ste-Foy, Quebec, Canada. pascal.imbeault@kin.msp.ulaval.ca
Tremblay
Angelo
A
Simoneau
Jean-Aimé
JA
Joanisse
Denis R
DR
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000273:Adipose Tissue; D001140:Aroclors / Q000097:blood; D001823:Body Composition; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003633:Dichlorodiphenyl Dichloroethylene / Q000097:blood; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006019:Glycolysis; D006801:Humans; D007306:Insecticides / Q000097:blood*; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D025221:Phosphofructokinases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000097:blood; D012727:Sex Characteristics; D015431:Weight Loss
D001140:Aroclors; D007306:Insecticides; C026987:aroclor 1260; D003633:Dichlorodiphenyl Dichloroethylene; D011078:Polychlorinated Biphenyls; D015094:3-Hydroxyacyl CoA Dehydrogenases; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D025221:Phosphofructokinases; C014024:2,4,5,2',4',5'-hexachlorobiphenyl