[HIV-1 Tat protein induces IL-10 production by human monocytes: implications of the PKC and calcium pathway].
In asymptomatic patients infected by HIV-1, the level of IL-10, a cytokine with immunosuppressive activity, is associated with the course of HIV infection towards AIDS. We show that HIV-1 Tat, a viral protein secreted by infected cells, induces IL-10 production by human peripheral blood monocytes. The analysis of the signal transduction pathways strongly suggests that the protein kinase C may play an essential role in this induction. Stimulation by Tat induces nuclear translocation of the transcription factor NFkB the activation of which seems to be necessary for IL-10 production. Using microspectrofluorimetry and confocal microscopy, we also show that Tat induces a calcium influx.
Bennasser
Y
Y
Laboratoire d'Immuno-Virologie, Université Paul Sabatier 118, route de Narbonne, 31062 Toulouse.
Yamina
B
B
Contreras
X
X
Xavier
C
C
Moreau
M
M
Marc
M
M
Le Clerc
C
C
Catherine
L
L
Badou
A
A
Abdallah
B
B
Bahraoui
E
E
D002118:Calcium / Q000378:metabolism*; D015696:Gene Products, tat / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D016328:NF-kappa B; D054322:tat Gene Products, Human Immunodeficiency Virus; D016753:Interleukin-10; D011493:Protein Kinase C; D002118:Calcium