Comparison of two methods for serotyping Ureaplasma urealyticum clinical isolates.
A newly developed enzyme linked immunosorbent assay (ELISA) method using monoclonal antibodies (MAbs) to the 14 serotypes of Ureaplasma urealyticum was compared to immunofluorescence assay (IFA) for serotyping U. urealyticum clinical isolates. Of the 102 vaginal isolates of U. urealyticum, five strains were lost and were excluded from analysis. Of the 97 strains analysed, a total of 86 (89%) strains were typeable by ELISA and a total of 89 (92%) strains were typeable by IFA. Eighty-six strains were typeable by both methods, three by IFA only and eight strains were not typeable neither by ELISA nor by IFA. Of the 86 strains typeable by both methods, complete concordance in serotyping results was found. The three strains not typeable by ELISA were typeable as serotype 4 by IFA. These three strains were reanalysed by ELISA after major modifications of the antigen preparation and were typeable as serotype 4. In conclusion, the ELISA was found suitable for serotyping clinical isolates. However, since the ELISA had a somewhat lower performance than IFA, strains not typeable by ELISA, should be retested by another technique such as IFA.
Echahidi
Fedoua
F
Department of Microbiology, Academisch Ziekenhuis, Vrije Universiteit Brussel, Laarbeeklaan 101 1090, Brussels, Belgium. labomicro@az.vub.ac.be
van Geel
Katrien
K
Lauwers
Sabine
S
Naessens
Anne
A
D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011247:Pregnancy; D016869:Ureaplasma Infections / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D014621:Vagina / Q000382:microbiology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal