Pancreatic phospholipase A2--mediated enhancement of the respiratory burst response of human neutrophils.
The aim of this study was to investigate the effects of exogenously added pancreatic phospholipase A2 (pPLA2) on the production of reactive oxygen species by human polymorphonuclear leukocytes (PMNs). Pancreatic PLA2 was used because PMNs do not possess a receptor for that enzyme and, therefore, the receptor-mediated effects could be excluded. Respiratory burst activity of PMNs was monitored by luminol-amplified chemiluminescence and the lipid composition of neutrophils after treatment with pPLA2 was determined by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Our results show that the products of the pPLA2 digestion of the PMN membrane--lysophospholipids and the corresponding free fatty acids--significantly enhanced the respiratory burst response of human neutrophils.
Müller
J
J
Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Germany.
Petković
M
M
Schiller
J
J
Arnhold
J
J
D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D010179:Pancreas / Q000201:enzymology*; D010741:Phospholipases A / Q000097:blood*; D054467:Phospholipases A2; D016897:Respiratory Burst / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010741:Phospholipases A; D054467:Phospholipases A2