Nitric oxide regulates matrix metalloprotease-13 expression and activity in endothelium.
BACKGROUND
Matrix metalloproteinases (MMPs) are synthesized in response to diverse stimuli including cytokines, growth factors, hormones, and oxidative stress.


METHODS
Bovine aortic endothelial cells (BAEC) were stimulated with nitric oxide (NO) and MMP-13 expression and activity was assayed.


RESULTS
NO transcriptionally regulated matrix metalloproteinase-13 (MMP-13) expression in BAEC, while the cGMP analog 8Br-cGMP mimicked the effect of NO. In addition NO also stimulated the proteolytic processing of MMP-13 from the pro-enzyme to the final active form in a dose dependent manner.


CONCLUSIONS
NO transcriptionally regulates MMP expression and activity in the vascular endothelium. This effect of NO may be of pathophysiological importance in the context of angiogenesis, inflammation or atherogenesis.
Zaragoza
Carlos
C
Centro de Investigaciones Biológicas, Instituto "Reina Sofía" de Investigaciones Nefrológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006 Madrid, Spain. carlosz@cib.csic.es
Balbín
Milagros
M
López-Otín
Carlos
C
Lamas
Santiago
S
D000818:Animals; D002417:Cattle; D017364:Collagenases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D006834:Hydrazines / Q000008:administration & dosage / Q000494:pharmacology; D053509:Matrix Metalloproteinase 13; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000008:administration & dosage / Q000494:pharmacology; D009589:Nitrogen Oxides
D006834:Hydrazines; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; D009569:Nitric Oxide; C084012:1,1-diethyl-2-hydroxy-2-nitrosohydrazine; D017364:Collagenases; D053509:Matrix Metalloproteinase 13