Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast.
Yeast produce two classes of secretory vesicles (SVs) that differ in both density and cargo protein content. In late-acting secretory mutants (e.g. snc1(ala43) and sec6-4), both low- (LDSV) and high-density (HDSV) classes of vesicles accumulate at restrictive temperatures. Here, we have found that disruptions in the genes encoding a dynamin-related protein (VPS1) or clathrin heavy chain (CHC1) abolish HDSV production, yielding LDSVs that contain all secreted cargos. Interestingly, disruption of the PEP12 gene, which encodes the t-SNARE that mediates all Golgi to pre-vacuolar compartment (PVC) transport, also abolishes HDSV production. In contrast, deletions in genes that selectively confer vacuolar hydrolase sorting to the PVC or protein transport to the vacuole (i.e. VPS34 and VAM3, respectively) have no effect. Thus, one branch of the secretory pathway in yeast involves an intermediate sorting compartment and has a specific requirement for clathrin and a dynamin-related protein in SV biogenesis.
Gurunathan
Sangiliyandi
S
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
David
Doris
D
Gerst
Jeffrey E
JE
D002352:Carrier Proteins / Q000235:genetics; D002966:Clathrin / Q000502:physiology*; D034281:Dynamins; D011992:Endosomes / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000502:physiology*; D019204:GTP-Binding Proteins; D005800:Genes, Fungal; D006056:Golgi Apparatus / Q000378:metabolism; D008854:Microscopy, Electron; D009154:Mutation; D015388:Organelles / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D013696:Temperature; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D002966:Clathrin; D033921:Vesicular Transport Proteins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C064553:VPS1 protein, S cerevisiae; D034281:Dynamins