[Transplantation of neural retina after photic injury of retina in mice].
OBJECTIVE
To examine the possibility of retinal photoreceptors rescued after photic injury by allogeneic neural retinal transplantation.


METHODS
The retinal photic injury model was established by a light-damage trunk designed by ourselves. After photo-illumination, 60 mice were divided into two groups: Group I (n = 20) was the photic injury group and group II (n = 40), the retinal transplantation group; then group II was subdivided into II(A) (n = 20) and II(B) (n = 20) subgroups. External approach for neural retinal transplantation was performed at 24hr (group II(A)) and day 7 (group II(B)) after photic injury respectively. The recipients were sacrificed at day 6, day 12, day 18 and day 30 post-operatively to examine whether the graft could rescue the injured retinal photoreceptors.


RESULTS
In group I, the inner and outer segment showed edema, disorganization and vesiculation, the outer nuclear layer became very thin and disorganized, and the cell nuclear chromatin showed pyknosis and margination. In group II, the graft survival rate in group II(A) was 50% (10/20) and in group II(B) was 80% (16/20), the difference being significant (chi(2) = 3.956, P < 0.05). Among the graft survival samples, we compared the rescue rate of photoreceptors between group II(A) 40% (4/10) and group II(B) 50% (8/16), the difference being not significant (P = 0.702).


CONCLUSIONS
The retinal photic injury may result in photoreceptor degeneration that can be rescued by retinal transplantation, and the successful rate of the transplantation performed at day 7 is higher than that at 24-hr after injury.
Peng
Q
Q
The First Hospital of Shanxi Medical University, Taiyuan 030001.
Ren
P
P
Liu
S
S
D000818:Animals; D005260:Female; D051379:Mice; D010786:Photoreceptor Cells / Q000473:pathology*; D011833:Radiation Injuries, Experimental / Q000601:surgery*; D012160:Retina / Q000637:transplantation*; D012162:Retinal Degeneration / Q000601:surgery