title: Monoamine oxidase inhibitory coumarin from Zanthoxylum schinifolium.
abstract: A methanol extract of Zanthoxylum schinifolium stems at a concentration of 250 microg/ml showed potent inhibitory activity against monoamine oxidase (MAO) in a mouse brain. Activity-guided separation and purification of the extract yielded lacinartin (1) as an active coumarin compound. Lacinartin showed significant inhibitory effects on MAO in a dose-dependent manner. The IC(50) value on MAO activity was 9.2 microM. The MAO-A (IC(50) value, 5.7 microM) sensitivity to lacinartin was greater than that of MAO-B (IC(50) value, 28.6 microM). An enzyme kinetic study revealed that lacinartin inhibited MAO activity by a non-competitive mode.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, Korea.
authors.forename: Young Su
authors.initials: YS
authors.lastname: Jo
authors.forename: Dang Thi Lan
authors.initials: DT
authors.lastname: Huong
authors.forename: KiHwan
authors.initials: K
authors.lastname: Bae
authors.forename: Myung Koo
authors.initials: MK
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D008297:Male; D051379:Mice; D008995:Monoamine Oxidase / Q000187:drug effects / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027821:Rutaceae
chemical_list: D003374:Coumarins; D008996:Monoamine Oxidase Inhibitors; D010936:Plant Extracts; C452988:lacinartin; C030123:coumarin; D008995:Monoamine Oxidase