title: Suppression of tumor growth and pulmonary metastasis in murine osteosarcoma using gene therapy.
abstract: We evaluated the effect of gene therapy in the murine osteosarcoma cell line, LM8, which preferentially metastasizes to the lungs. LM8 cells were transduced with the gene for a herpes simplex virus thymidine kinase (HSV-tk) or Escherichia coli beta-galactosidase (lacZ). We investigated the cytotoxicity of LM8 cells bearing an HSV-tk gene after treatment with ganciclovir (GCV). LM8 cells bearing an HSV-tk gene were more sensitive than non-transduced cells. The remarkable inhibition of tumor growth and pulmonary metastases was confirmed in vivo. Our findings indicated that GCV kills tumor cells transduced with HSV-tk in vitro and in vivo.
authors.affiliation: Department of Orthopaedic Surgery, Mie University School of Medicine, Tsu city, Mie prefecture 514, Japan. m-seto@clin.medic.mie-u.ac.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Seto
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Jun
authors.initials: J
authors.lastname: Sonoda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Matsumine
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Shinto
authors.forename: Atsumasa
authors.initials: A
authors.lastname: Uchida
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D015774:Ganciclovir / Q000627:therapeutic use; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000556:secondary / Q000628:therapy*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000235:genetics / Q000556:secondary / Q000628:therapy*; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D015996:Survival Rate; D013937:Thymidine Kinase / Q000235:genetics; D014161:Transduction, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir