EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family.
The eds5 mutant of Arabidopsis (earlier named sid1) was shown previously to accumulate very little salicylic acid and PR-1 transcript after pathogen inoculation and to be hypersusceptible to pathogens. We have isolated EDS5 by positional cloning and show that it encodes a protein with a predicted series of nine to 11 membrane-spanning domains and a coil domain at the N terminus. EDS5 is homologous with members of the MATE (multidrug and toxin extrusion) transporter family. EDS5 expression is very low in unstressed plants and strongly induced by pathogens and UV-C light. The transcript starts to accumulate 2 hr after inoculation of Arabidopsis with an avirulent strain of Pseudomonas syringae or UV-C light exposure, and it stays induced for approximately 2 days. EDS5 also is expressed after treatments with salicylic acid, indicating a possible positive feedback regulation. EDS5 expression after infection by certain pathogens as well as after UV-C light exposure depends on the pathogen response proteins EDS1, PAD4, and NDR1, indicating that the signal transduction pathways after UV-C light exposure and pathogen inoculation share common elements.
Nawrath
Christiane
C
Department of Biology, Unit of Plant Biology, Route Albert Gockel 3, 1700 Fribourg, Switzerland. christiane.nawrath@unifr.ch
Heck
Silvia
S
Parinthawong
Nonglak
N
Métraux
Jean-Pierre
JP
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D001692:Biological Transport; D002265:Carboxylic Ester Hydrolases / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D007113:Immunity, Innate / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D011549:Pseudomonas / Q000254:growth & development; D020156:Salicylic Acid / Q000494:pharmacology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics; D014466:Ultraviolet Rays
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C118837:EDS1 protein, Arabidopsis; C455514:EDS5 protein, Arabidopsis; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C109369:NDR1 protein, Arabidopsis; D014157:Transcription Factors; D002265:Carboxylic Ester Hydrolases; C400766:PAD4 protein, Arabidopsis; D020156:Salicylic Acid