Functional cell-based uHTS in chemical genomic drug discovery.
The availability of genomic information significantly increases the number of potential targets available for drug discovery, although the function of many targets and their relationship to disease is unknown. In a chemical genomic research approach, ultra-high throughput screening (uHTS) of genomic targets takes place early in the drug discovery process, before target validation. Target-selective modulators then provide drug leads and pharmacological research tools to validate target function. Effective implementation of a chemical genomic strategy requires assays that can perform uHTS for large numbers of genomic targets. Cell-based functional assays are capable of the uHTS throughput required for chemical genomic research, and their functional nature provides distinct advantages over ligand-binding assays in the identification of target-selective modulators.
Croston
Glenn E
GE
ACADIA Pharmaceuticals, 3911 Sorrento Valley Blvd, San Diego, CA 92121, USA. gcroston@acadia-pharm.com
D001667:Binding, Competitive / Q000502:physiology; D002478:Cells, Cultured; D002621:Chemistry / Q000379:methods; D019295:Computational Biology / Q000379:methods; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004347:Drug Interactions / Q000502:physiology; D023281:Genomics / Q000379:methods*; D006801:Humans; D015398:Signal Transduction / Q000502:physiology