[The modulating effect of panax pseudoginseng wall saponins on the DAG-PKC signal pathway and TNF secretion of macrophages].
OBJECTIVE
To explore the roles of panax pseudoginseng wall saponins on the DAG-PKC signal pathway and TNF secretion of macrophages.


METHODS
The changes of the activities of postburn inositol lipid signal system factors such as PLC, DAG, cytomembrane PKC, cytoplasma PKC, and intracellular calcium concentration and the secretory amount of macrophage TNF were observed.


RESULTS
Panax pseudoginseng wall saponins could reduce the increased PLC activity from 57.58 +/- 8.19 to 27.00 +/- 2.31 and the intracellular calcium concentration from 393.18 +/- 392.62 to 90.56 +/- 7.21. With the role of panax pseudoginseng wall saponins, DAG activity reduced from 488.10 +/- 40.20 to 288.30 +/- 30.00, cytomembrane PKC activity decreased from 3081.50 +/- 698.50 to 1699.50 +/- 218.50, and cytoplasmic PKC activity from 2 188.60 +/- 258.30 to 848.40 +/- 138.30. The secretory TNF amount of macrophage decreased by 55%.


CONCLUSIONS
Panax pseudoginseng wall saponins might play an very important role in the modulating of the DAG-PKC signal pathway and decreased TNF secretion of macrophage.
Luo
Z
Z
Burn Research Institute, Southwestern Hospital, Third Military Medical University, Chongqing, 400038, China.
Cai
S
S
Huang
W
W
He
B
B
Liu
J
J
Zhou
X
X
Fu
Y
Y
Chen
X
X
Li
A
A
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000503:physiopathology; D004075:Diglycerides / Q000378:metabolism*; D004195:Disease Models, Animal; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D005894:Panax; D008517:Phytotherapy; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
C011439:1,2-diacylglycerol; D004075:Diglycerides; D014409:Tumor Necrosis Factor-alpha; D011493:Protein Kinase C; D010738:Type C Phospholipases