title: Dramatic increase in lymph node dendritic cell number during infection by the mouse mammary tumor virus occurs by a CD62L-dependent blood-borne DC recruitment.
abstract: Despite the information dealing with the differential phenotype and function of the main mouse dendritic cell (DC) subpopulations, namely, CD8alpha(-) and CD8alpha(+) DCs, their origin and involvement in antiviral immune responses in vivo are still largely unknown. To address these issues, this study used the changes occurring in DC subpopulations during the experimental infection by the Swiss (SW) strain of the mouse mammary tumor virus (MMTV). MMTV(SW) induced an 18-fold increase in lymph node DCs, which can be blocked by anti-CD62L treatment, concomitant with the presence of high numbers of DCs in the outer cortex, in close association with high endothelial venules. These data suggest that the DC increase caused by MMTV(SW) infection results from the recruitment of blood-borne DCs via high endothelial venules, by a CD62L-dependent mechanism. In addition, skin sensitization assays indicate that MMTV(SW) infection inhibits epidermal Langerhans cell migration to the draining lymph node. Moreover, data on the kinetics of MMTV(SW)-induced expansion of the different DC subsets support the hypothesis that CD8(-) and CD8(+) DCs represent different maturation stages of the same DC population, rather than myeloid- and lymphoid-derived DCs, respectively, as previously proposed. Finally, the fact that DCs were infected by MMTV(SW) suggests their participation in the early phases of infection.
authors.affiliation: Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Martín
authors.forename: Sara Ruiz
authors.initials: SR
authors.lastname: Ruiz
authors.forename: Gloria Martínez
authors.initials: GM
authors.lastname: del Hoyo
authors.forename: Fabienne
authors.initials: F
authors.lastname: Anjuère
authors.forename: Héctor Hernández
authors.initials: HH
authors.lastname: Vargas
authors.forename: María
authors.initials: M
authors.lastname: López-Bravo
authors.forename: Carlos
authors.initials: C
authors.lastname: Ardavín
mesh_terms: D000818:Animals; D016827:CD8 Antigens / Q000032:analysis; D002452:Cell Count; D002454:Cell Differentiation; D002633:Chemotaxis / Q000187:drug effects* / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology / Q000821:virology*; D016130:Immunophenotyping; D019041:L-Selectin / Q000494:pharmacology* / Q000502:physiology; D008198:Lymph Nodes / Q000473:pathology* / Q000821:virology; D008208:Lymphatic System; D008324:Mammary Tumor Virus, Mouse; D051379:Mice; D008807:Mice, Inbred BALB C; D012192:Retroviridae Infections / Q000276:immunology / Q000473:pathology*; D014412:Tumor Virus Infections / Q000276:immunology / Q000473:pathology*
chemical_list: D016827:CD8 Antigens; D019041:L-Selectin