title: A correlative study on antimutagenic and chemopreventive activity of Acacia auriculiformis A. Cunn. and Acacia nilotica (L.) Willd. Ex Del.
abstract: The present study provides a correlation of the antimutagenic and chemopreventive activity of the barks of two commonly observed plants viz. Acacia auriculiformis and Acacia nilotica. We used the Ames antimutagenicity assay and the mouse mammary gland organ culture (MMOC) model. The plants were extracted with organic solvents to obtain chloroform fractions and acetone extracts. The antimutagenic activity was determined in two different strains using both direct-acting [4-nitro-o-phenylenediamine (NPD) or sodium azide] and indirect-acting [2-aminofluorene (2AF)] mutagens. The anticarcinogenic activity was evaluated based on the development of preneoplastic lesions in response to the chemical carcinogen 7,12-dimethylbenz[a]anthracene (DMBA). The results showed that the activity resulting from the 2AF mutagen was selectively greater than the activity from the direct-acting mutagens. Moreover, in general, acetone extracts were more potent in suppressing mutagenesis than the chloroform extracts. The antimutagenicity results obtained with extracts using the 2AF--TA100 system were comparable to the chemopreventive results with DMBA-induced mammary lesions. The order of activity in both tests was A. nilotica > A. auriculiformis. These results exhibited a good correlation between the antimutagenesis assay and the MMOC model, suggesting that these plants may contain active chemopreventive agents.
authors.affiliation: Department of Botanical Sciences, Guru Nanak Dev University, Amritsar 143 005, India. kamal_rajput@hotmail.com
authors.forename: Kamaljit
authors.initials: K
authors.lastname: Kaur
authors.forename: Saroj
authors.initials: S
authors.lastname: Arora
authors.forename: Michael E
authors.initials: ME
authors.lastname: Hawthorne
authors.forename: Swayamjot
authors.initials: S
authors.lastname: Kaur
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
authors.forename: Rajendra G
authors.initials: RG
authors.lastname: Mehta
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000045:Acacia; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005449:Fluorenes / Q000633:toxicity; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens / Q000633:toxicity; D010655:Phenylenediamines / Q000633:toxicity; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011230:Precancerous Conditions / Q000139:chemically induced / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D019810:Sodium Azide / Q000633:toxicity
chemical_list: D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D005449:Fluorenes; D009153:Mutagens; D010655:Phenylenediamines; D010936:Plant Extracts; C012177:2-aminofluorene; D015127:9,10-Dimethyl-1,2-benzanthracene; C012277:1,2-diamino-4-nitrobenzene; D019810:Sodium Azide