MMA/MPEOMA/VSA copolymer as a novel blood-compatible material: effect of PEO and negatively charged side chains on protein adsorption and platelet adhesion.
This study was designed to evaluate the effect of polyethylene oxide (PEO) and negatively charged side chains on blood compatibility. For this, novel copolymers (MMA/MPEOMA/VSA copolymers) with both PEO and negatively chargeable side chains were synthesized by random copolymerization of methyl methacrylate (MMA), methoxy PEO monomethacrylate (MPEOMA; PEO mol wt 1000), and vinyl sulfonic acid sodium salt (VSA) monomers of different compositions. MMA/MPEOMA copolymer (with PEO side chains) and MMA/VSA copolymer (with negatively chargeable side groups) also were synthesized for purposes of comparison. The synthesized copolymers were characterized by 1H-nuclear magnetic resonance spectroscopy and gel permeation chromatography. They were coated onto polyurethane (PU) or polymethyl methacrylate (PMMA) films by spin coating. The surface properties of MMA/MPEOMA/VSA copolymers were compared by water contact angle and zeta potential with those of MMA/MPEOMA and MMA/VSA copolymers of similar MPEOMA or VSA composition. Using electron spectroscopy for chemical analysis and scanning electron microscopy, respectively, the behaviors of the adsorption of blood proteins (albumin, gamma-globulin, fibrinogen, and plasma proteins) and the adhesion of platelets on the copolymer-coated surfaces also were compared. Among the copolymers, the MMA/MPEOMA/VSA copolymer with a monomer molar ratio 8:1:1 was observed to be particularly effective in preventing both protein adsorption and platelet adhesion on the surfaces, probably owing to the combined effects of highly mobile, hydrophilic PEO side chains and negatively charged side groups in aqueous solution.
Lee
Jin Ho
JH
Department of Polymer Science and Engineering, Hannam University, 133 Ojeong Dong, Daedeog Ku, Taejon 306-791, Korea. jhlee@mail.hannam.ac.kr
Oh
Se Heang
SH
D000327:Adsorption; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001769:Blood / Q000187:drug effects*; D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D008970:Molecular Weight; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties
D001672:Biocompatible Materials; D001798:Blood Proteins; D008689:Methacrylates; C454117:poly(methyl methacrylate-co-methoxy polyethylene oxide monomethacrylate-co-vinyl sulfonic acid sodium salt); D011092:Polyethylene Glycols