Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei.
OBJECTIVE
To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei.


RESULTS
Humicola grisea xylanase 2 (xyn2) cDNA was expressed in Trichoderma reesei under the main cellobiohydrolase I (cbh1) promoter (i) as a fusion to the cellobiohydrolase I (CBHI) secretion signal and (ii) the mature CBHI core-linker. The recombinant xylanase (HXYN2) was secreted into the cultivation medium and processed in a similar fashion to the endogenous T. reesei xylanases, resulting in an active enzyme.


CONCLUSIONS
HXYN2 was successfully processed in T. reesei. Composition of the culture medium affected the HXYN2 yields, favouring Avicel-lactose as a carbon source. Best yields (about 0.5 g l(-1)) in shake flask cultivations were obtained from a transformant where xyn2 was fused directly to the CBHI secretion signal.
de Faria
F P
FP
Departamento de Biotecnologia, FAENQUIL, Lorena-SP, Brazil.
Te'O
V S J
VS
Bergquist
P L
PL
Azevedo
M O
MO
Nevalainen
K M H
KM
D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media; D006358:Hot Temperature; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase