title: Effect of dietary carbamyl phosphate on dentine apposition in rat molars.
abstract: In rats, sucrose increases dental caries and impairs odontoblastic function by reducing dentine apposition during primary dentinogenesis. A high-sucrose diet also affects negatively a pulp or dentine function that appears to regulate solute or fluid movement within rat dentinal tissue. In earlier work it was found that carbamyl phosphate could significantly reverse sucrose-induced cariogenesis and also stimulate sucrose-depressed movement of dentinal fluid through a mechanism involving parotid function(s). In the current study, the possibility that carbamyl phosphate could overcome the sucrose-induced reduction in dentine apposition was examined. Weanling rats were fed a high-sucrose diet supplemented or not with carbamyl phosphate for 5 weeks. Dentine apposition was measured planimetrically in sagittal sections of the molars. The effect of carbamyl phosphate was similarly tested in parotidectomized animals. Carbamyl phosphate significantly reduced the deleterious effect of sucrose on dentine apposition by 58% in the first molars. However, the reduction in dentine apposition that followed parotidectomy was not altered by carbamyl phosphate supplementation. The possibility that the beneficial effect of carbamyl phosphate on dentinogenesis involves a parotid function is entertained.
authors.affiliation: Department of Physiology/Pharmacology, Loma Linda University, Loma Linda, CA 92350, USA. jleonora@som.llu.edu
authors.forename: John
authors.initials: J
authors.lastname: Leonora
authors.forename: Leo
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Tieche
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002221:Carbamyl Phosphate / Q000494:pharmacology*; D003810:Dentinogenesis / Q000187:drug effects*; D019422:Dietary Sucrose / Q000009:adverse effects; D008297:Male; D010306:Parotid Gland / Q000502:physiology* / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
chemical_list: D019422:Dietary Sucrose; D002221:Carbamyl Phosphate