The human T-type amino acid transporter-1: characterization, gene organization, and chromosomal location.
System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A10). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.
Kim
Do Kyung
DK
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
Kanai
Yoshikatsu
Y
Matsuo
Hirotaka
H
Kim
Ju Young
JY
Chairoungdua
Arthit
A
Kobayashi
Yukari
Y
Enomoto
Atsushi
A
Cha
Seok Ho
SH
Goya
Tomoyuki
T
Endou
Hitoshi
H
D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes; D051381:Rats; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014982:Xenopus laevis
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; C498964:SLC16A10 protein, human; C429535:Slc16a10 protein, rat