Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities.
A fucose-containing glycoprotein fraction which stimulates spleen cell proliferation and cytokine expression has been identified from the water-soluble extract of Ganoderma lucidum. Proteomic analysis of mouse spleen cells treated with this glycoprotein fraction showed approximately 50% change of the proteome. Further studies on the activities of this glycoprotein fraction through selective proteolysis and glycosidic cleavage indicate that a fucose containing polysaccharide fraction is responsible for stimulating the expression of cytokines, especially IL-1, IL-2 and INF-gamma.
Wang
Yuan-Yuan
YY
Laboratory of Bioorganic Chemistry, Institute of Chemistry, Academia Sinica, Taipei, Taiwan.
Khoo
Kay-Hooi
KH
Chen
Shui-Tein
ST
Lin
Chun-Cheng
CC
Wong
Chi-Huey
CH
Lin
Chun-Hung
CH
D000276:Adjuvants, Immunologic / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D016172:DNA Fingerprinting; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D005643:Fucose; D006023:Glycoproteins / Q000032:analysis / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000032:analysis / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D000939:Epitopes; D006023:Glycoproteins; D011134:Polysaccharides; D005643:Fucose