Vibrio cholerae-loaded poly(DL lactide co-glycolide) microparticles.
Vibrio cholerae (VC)-loaded microparticles were prepared using poly(DL lactide-co-glycolide) with a water-in-oil-in-water emulsion/solvent extraction technique. Particle characteristics including size distribution, VC-loading efficiencies, and in-vitro release pattern were investigated. The dispersed phase was PLG dissolved in dichloromethane, and the continuous phase was water containing PVP as a stabilizer with varied sodium chloride concentrations. VC was successfully entrapped in the microparticles with trapping efficiencies up to 97.8%, a loading level of 55.4 g/mg, and particle size of 3.8 microm. Using 10% w/v PVP with 5% w/v NaCl in the continuous phase resulted in a higher loading level (55.4 +/- 6.9 g/mg), loading efficiency (97.8%), core region content (25.7 +/- 1.9 g/mg) and lower surface content (6.2 +/- 0.9 g/mg) than without NaCl (loading content: 40.7 +/- 5.1 g/mg; loading efficiency 52.1%; core region content: 8.3 +/- 0.5 g/mg; surface content: 19.5 +/- 1.1 g/mg). A linear release profile from VC-loaded microparticles was found. A preliminary animal oral administration study indicated that the VC-loaded microparticles, as an oral delivery system, have shown effective immunogencity in rats for 2 months. The VC incorporation and physicochemical characterization data obtained in this study may be relevant in optimising the vaccine incorporation and delivery properties of these potential vaccine targeting carriers.
Yeht
M K
MK
Department of Clinical Pharmacy, Tri-service General Hospital, National Defense Medical Center, Taipei, Taiwan. mkyeh@ndmctsgh.edu.tw
Chen
J L
JL
Chiang
C H
CH
D000284:Administration, Oral; D000818:Animals; D002771:Cholera / Q000188:drug therapy / Q000517:prevention & control; D004339:Drug Compounding / Q000379:methods*; D007700:Kinetics; D019344:Lactic Acid; D010316:Particle Size; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D014734:Vibrio cholerae / Q000276:immunology*
D011108:Polymers; D014612:Vaccines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012965:Sodium Chloride