title: The vaccinia virus soluble interferon-gamma receptor is a homodimer.
abstract: The vaccinia virus (VV) interferon (IFN)-gamma receptor (IFN-gammaR) is a 43 kDa soluble glycoprotein that is secreted from infected cells early during infection. Here we demonstrate that the IFN-gammaR from VV, cowpox virus and camelpox virus exists naturally as a homodimer, whereas the cellular IFN-gammaR dimerizes only upon binding the homodimeric IFN-gamma. The existence of the virus protein as a dimer in the absence of ligand may provide an advantage to the virus in efficient binding and inhibition of IFN-gamma in solution.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK1.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alcamí
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK1.
authors.forename: Geoffrey L
authors.initials: GL
authors.lastname: Smith
mesh_terms: D000818:Animals; D002162:Camelus / Q000821:virology; D002460:Cell Line; D015608:Cowpox virus / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000378:metabolism; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D017471:Receptors, Interferon / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins; D012995:Solubility; D012996:Solutions; D014616:Vaccinia virus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*; D000096743:Interferon gamma Receptor
chemical_list: D003432:Cross-Linking Reagents; D004220:Disulfides; D007371:Interferon-gamma; D021122:Protein Subunits; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D012996:Solutions; D014764:Viral Proteins; D000096743:Interferon gamma Receptor