title: Absorption studies on aminoglycoside binding to the packaging region of human immunodeficiency virus type-1.
abstract: The binding of paromomycin, neomycin B, and three analogues of neomycin to a 176-mer RNA from the packaging region of HIV-1 (LAI) has been studied using UV absorption spectroscopy at wavelengths between 200 and 300 nm. From plots of absorption as a function of drug concentration, values of binding constants for these drugs on RNA were determined.
authors.affiliation: Department of Chemistry, CST 1-014, Syracuse University, Syracuse, NY 13244, USA.
authors.forename: Julie M
authors.initials: JM
authors.lastname: Sullivan
authors.forename: Jerry
authors.initials: J
authors.lastname: Goodisman
authors.forename: James C
authors.initials: JC
authors.lastname: Dabrowiak
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D005601:Framycetin / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009355:Neomycin / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010303:Paromomycin / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D013057:Spectrum Analysis; D019065:Virus Assembly / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D005601:Framycetin; D010303:Paromomycin; D012313:RNA; D009355:Neomycin