[Effect of protein expression on antisense c-myc and PCNA for vascular smooth muscle cells].
OBJECTIVE
To study the methods of inhibiting proliferation of vascular smooth muscle cells (VSMCs), we observed the change of protein expression after using antisense c-myc, PCNA for VSMCs.


METHODS
The sense, antisense, and mismatched oligomers for c-myc or PCNA were individually delivered to the cultured VSMCs. Using Western blot, immunohistochemistry assay and image analysis system, we observed the change of c-myc and PCNA protein expression in the VSMCs.


RESULTS
Antisense c-myc, PCNA at concentration of 10 micromol/L can successfully inhibit the expression of c-myc, PCNA protein in the VSMCs (P < 0.05), but the sense and the mismatched do not have this effect (P > 0.05).


CONCLUSIONS
The data suggested that the antisense c-myc, PCNA could attenuate the protein expression of c-myc, PCNA, and it is an effective method for the inhibition of VSMCs hyperplasia.
Bian
J
J
Department of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Zhang
B
B
Qian
H
H
Chen
S
S
D015153:Blotting, Western; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D016376:Oligonucleotides, Antisense; D018809:Proliferating Cell Nuclear Antigen; D016271:Proto-Oncogene Proteins c-myc