Protective effects of puerariaeflos against ethanol-induced apoptosis on human neuroblastoma cell line SK-N-MC.
Puerariaeflos (PF) is an oriental medical herb for alcohol abuse. To investigate whether PF possesses protective effects against ethanol (EtOH)-induced cytotoxicity in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometric analysis, DNA fragmentation assay, and reverse transcription-polymerase chain reaction were performed on SK-N-MC human neuroblastoma cells. Cells treated with EtOH exhibited several apoptotic features, while those pre-treated with PF prior to EtOH exposure showed a decreased occurrence of apoptotic features. In addition, PF pre-treatment inhibited the EtOH-induced increase in caspase-3 mRNA expression. These results suggest that PF may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.
Jang
M H
MH
Departments of Physiology, College of Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, Korea.
Shin
M C
MC
Kim
Y J
YJ
Chung
J H
JH
Yim
S V
SV
Kim
E H
EH
Kim
Y
Y
Kim
C J
CJ
D000427:Alcohol Deterrents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001932:Brain Neoplasms / Q000473:pathology*; D053938:DNA Fragmentation / Q000187:drug effects; D000431:Ethanol / Q000037:antagonists & inhibitors*; D005434:Flow Cytometry; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D029908:Pueraria / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000427:Alcohol Deterrents; D010936:Plant Extracts; D000431:Ethanol