[Effect of wear particles on cell-to-cell communication of synoviocyte system in vitro and its significance in aseptic loosening of prosthesis].
OBJECTIVE
To study the biological reason of abnormal fiber proliferation at bone-implant interface of aseptically loosened prosthesis by observing cell-to-cell communication of synoviocyte system in vitro under the stimulation of wear particles.


METHODS
The synoviocyte system of normal human hip joint was established in vitro and the change of cell-to-cell communication was monitored by fluorescence redistribution after photobleaching technique (FRAP) and confocal laser scanning microscope (CLSM) after Ti alloy, CoCr alloy or UHMWPE particles suspension (1.5 mg/ml, W/V) was added into the system.


RESULTS
Cell-to-cell communication of synoviocyte system significantly decreased under the stimulation of three kinds of particles (P < 0.01). he decreased degree was maximal in UHMWPE group and minimal in Ti alloy group (P < 0.01). The cell-to-cell communication level of fibroblast-like-cells (FCs) was significantly lower than that of macrophage-like-cells (MCs) (P < 0.05).


CONCLUSIONS
Besides the effect of cytokines promoting fiber proliferation, the great amount of fibrous granuloma at bone-implant interface which has relations with aseptic loosening may be caused by the decrease of cell-to-cell communication of fibroblast under direct stimulation of wear particles.
Cai
X
X
General Hospital of Chinese People's Liberation Army, Beijing 100853.
Wang
J
J
Hu
Y
Y
Lu
S
S
Li
N
N
Huang
J
J
D000497:Alloys; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D002858:Chromium Alloys / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D036681:Fluorescence Recovery After Photobleaching; D006621:Hip Joint / Q000473:pathology*; D006622:Hip Prosthesis; D006801:Humans; D008264:Macrophages / Q000502:physiology; D018613:Microscopy, Confocal; D010316:Particle Size; D011095:Polyethylenes / Q000494:pharmacology*; D011475:Prosthesis Failure; D013583:Synovial Membrane / Q000166:cytology* / Q000502:physiology; D014025:Titanium / Q000494:pharmacology
D000497:Alloys; D002858:Chromium Alloys; D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; C031462:titanium alloy (TiAl6V4); D014025:Titanium