title: Participation of Fas-mediated apoptotic pathway in KB, a human head and neck squamous cell carcinoma cell line, after irradiation.
abstract: In head and neck clinical oncology, recurrent cancer after initial irradiation therapy is no longer sensitive to irradiation. To explore the irradiation resistance in head and neck squamous cell carcinoma, a human cell line, KB, derived from the floor of the oral cavity was used. The participation of the Fas-mediated apoptotic pathway was suggested by the upregulation of the surface Fas molecule, the reduction of the apoptotic cell fraction after inhibition of caspase 8 which is a Fas-related initiator caspase, and the changes in Fas-related genes after irradiation. Therefore, it is suggested that disruption of the Fas-mediated apoptotic pathway participates in the acquisition of irradiation-resistance in HNSCC.
authors.affiliation: Department of Otorhinolaryngology, Kawasaki Medical School, Okayama 701-0192, Japan. unomako@med.kawasaki-m.ac.jp
authors.forename: Masako
authors.initials: M
authors.lastname: Uno
authors.forename: Takemi
authors.initials: T
authors.lastname: Otsuki
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Yata
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Fujii
authors.forename: Haruko
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Akisada
authors.forename: Junichi
authors.initials: J
authors.lastname: Hiratsuka
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Imajo
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Harada
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000532:radiotherapy*; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases / Q000096:biosynthesis; D002469:Cell Separation; D018076:DNA, Complementary / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000473:pathology* / Q000532:radiotherapy*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D019014:fas Receptor / Q000096:biosynthesis*
chemical_list: D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor; C505530:CASP8 protein, human; C506433:CASP9 protein, human; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases