title: Solution structure of the eukaryotic pore-forming cytolysin equinatoxin II: implications for pore formation.
abstract: Sea anemones produce a family of 18-20 kDa proteins, the actinoporins, that lyse cells by forming pores in cell membranes. Sphingomyelin plays an important role in their lytic activity, with membranes lacking this lipid being largely refractory to these toxins. The structure of the actinoporin equinatoxin II in aqueous solution, determined from NMR data, consists of two short helices packed against opposite faces of a beta-sandwich structure formed by two five-stranded beta-sheets. The protein core has extensive hydrophobic interfaces formed by residues projecting from the internal faces of the two beta-sheets. 15N relaxation data show uniform backbone dynamics, implying that equinatoxin II in solution is relatively rigid, except at the N terminus; its inferred rotational correlation time is consistent with values for monomeric proteins of similar mass. Backbone amide exchange rate data also support the view of a stable structure, even though equinatoxin II lacks disulfide bonds. As monitored by NMR, it unfolds at around 70 degrees C at pH 5.5. At 25 degrees C the structure is stable over the pH range 2.5-7.3 but below pH 2.5 it undergoes a slow transition to an incompletely unfolded structure resembling a molten globule. Equinatoxin II has two significant patches of positive electrostatic potential formed by surface-exposed Lys and Arg residues, which may assist its interaction with charged regions of the lipid head groups. Tyr and Trp residues on the surface may also contribute by interacting with the carbonyl groups of the acyl chains of target membranes. Data from mutational studies and truncated analogues identify two regions of the protein involved in membrane interactions, the N-terminal helix and the Trp-rich region. Once the protein is anchored, the N-terminal helix may penetrate the membrane, with up to four helices lining the pore, although other mechanisms of pore formation cannot be ruled out.
authors.affiliation: Biomolecular Research Institute, 343 Royal Parade, Parkville 3052, Australia.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Hinds
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Gregor
authors.initials: G
authors.lastname: Anderluh
authors.forename: Poul Erik
authors.initials: PE
authors.lastname: Hansen
authors.forename: Raymond S
authors.initials: RS
authors.lastname: Norton
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003064:Cnidarian Venoms / Q000737:chemistry* / Q000378:metabolism*; D003603:Cytotoxins / Q000737:chemistry* / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000737:chemistry* / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012615:Sea Anemones / Q000737:chemistry* / Q000166:cytology; D012996:Solutions; D055672:Static Electricity; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics
chemical_list: D003064:Cnidarian Venoms; D003603:Cytotoxins; D012996:Solutions; C012307:equinatoxin