Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors.
The effects of acute physiological stressors (5 s tail pinch, handling and forced swimming at +25 and +5 degrees C for 3 min each) on serotonin (5-HT) release in the mouse brain were investigated using in vivo microdialysis. The extracellular 5-HT levels were determined by a newly developed highly-sensitive and selective high-performance liquid chromatography method based on derivatization with benzylamine and fluorescence detection. The basal levels of 5-HT in 3 min microdialysates from the ventral hippocampus and frontal cortex were 0.68+/-0.21 and 0.75+/-0.28 fmol/6 microl (n=24), respectively. All three stressors caused an immediate, significant and reversible increase (handling: 150%; swimming: 240%) of extracellular 5-HT levels in both brain structures, suggesting a more dynamic role played by the serotonergic system in response to acute stress.
Fujino
Kaoru
K
Chemicals Evaluation and Research Institute, 3-822 Ishii Machi, Hita, Oita 877-0061, Japan.
Yoshitake
Takashi
T
Inoue
Osamu
O
Ibii
Nobuhiro
N
Kehr
Jan
J
Ishida
Junichi
J
Nohta
Hitoshi
H
Yamaguchi
Masatoshi
M
D000208:Acute Disease; D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D005625:Frontal Lobe / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D017551:Microdialysis; D009474:Neurons / Q000378:metabolism*; D010146:Pain / Q000378:metabolism / Q000503:physiopathology; D010812:Physical Stimulation; D012149:Restraint, Physical; D012701:Serotonin / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D013550:Swimming; D009435:Synaptic Transmission / Q000502:physiology
D012701:Serotonin