Quantitative RT-PCR assay for mRNA of VEGF and histone H4 in the determination of proliferative and angiogenic activity in vulvar pathology.
Proliferative and angiogenic activity of tissue specimens taken from women with various vulvar pathologies were evaluated by determining the number of mRNA VEGF molecules and H4 histone mRNA molecules, by means of the QRT-PCR (TaqMan) technique. Following a cluster analysis the results, where normalised. Euclidean distances were used, all the cases were classified into three groups of pathologies. Group I included low degree vulvar pathologies, group II included high degree vulvar pathologies and group III included vulvar pathologies with high proliferative and angiogenic activity. Significant differences were found in the proliferative and angiogenic activity between groups I and III, and between groups II and III, while no statistically significant differences were found between groups I and II.
Michalski
B
B
Department of Obstetrics and Gynecology, Medical University of Silesia, Tychy, Poland. bogdan.michalski@proloc.com.pl
Mazurek
U
U
Olejek
A
A
Graniczka
M
M
Loch
T
T
Poreba
R
R
Wilczok
T
T
D002278:Carcinoma in Situ / Q000473:pathology* / Q000503:physiopathology; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis; D016228:Endothelial Growth Factors / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000235:genetics*; D006801:Humans; D008222:Lymphokines / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000473:pathology* / Q000503:physiopathology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019914:Taq Polymerase; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014846:Vulvar Neoplasms / Q000473:pathology* / Q000503:physiopathology
D004273:DNA, Neoplasm; D016228:Endothelial Growth Factors; D006657:Histones; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D019914:Taq Polymerase