Structure of the major glycolipid from Rothia dentocariosa.
Structural studies of the major glycolipid isolated from Rothia dentocariosa were carried out by specific chemical degradation and nuclear magnetic resonance spectroscopy. The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside alpha-D-Manp-(1-->3)-alpha-D-Manp-(1-->3)-sn-Gro, and the internal mannose was esterified at C-6 by fatty acid residue. The other fatty acyl chain substituted the primary methylene position of glycerol. The occurrence of this glycolipid is limited to the related microorganisms. The structural characteristics can facilitate the differentiation of some genera.
Paściak
Mariola
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, PL-53-114 Wrocaw, Poland.
Ekiel
Irena
I
Grzegorzewicz
Anna
A
Mordarska
Halina
H
Gamian
Andrzej
A
D000096:Acetone; D000191:Actinomycetaceae / Q000145:classification; D001419:Bacteria / Q000737:chemistry* / Q000145:classification; D002725:Chloroform; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D000432:Methanol
D006017:Glycolipids; C031647:dimannosyl diacylglycerol; D000096:Acetone; D002725:Chloroform; D000432:Methanol