title: (+)-Alpha-viniferin, a stilbene trimer from Caragana chamlague, inhibits acetylcholinesterase.
abstract: In the course of screening natural products for anti-acetylcholinesterase (AChE) activity, we found that a total methanolic extract of the underground parts of Caragana chamlague (Leguminosae) had significant inhibition towards AChE. Bioactivity-guided fractionation of the total methanolic extract resulted in the isolation and identification of two active stilbene oligomers, (+)-alpha-viniferin (1) and kobophenol A (2). Both 1 and 2 inhibited AChE activity in a dose-dependent manner, and the IC50 values of 1 and 2 were 2.0 and 115.8 microM, respectively. The AChE inhibitory activity of 1 was specific, reversible and noncompetitive.
authors.affiliation: College of Pharmacy, Seoul National University, Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
authors.forename: So Young
authors.initials: SY
authors.lastname: Kang
authors.forename: Ki Yong
authors.initials: KY
authors.lastname: Lee
authors.forename: Mi Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong Hee
authors.initials: JH
authors.lastname: Park
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Jinwoong
authors.initials: J
authors.lastname: Kim
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001572:Benzofurans; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D013267:Stilbenes; C064176:alpha-viniferin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase