Effect of dimethoate on the function and expression of nicotinic acetylcholine receptor in primary skeletal muscle cell culture.
To investigate the molecular mechanism of intermediate myasthenia syndrome (IMS), we analyzed the toxic effects of the representative organophosphate dimethoate on the function and expression of the nicotinic acetylcholine receptor (nAChR) in primary skeletal muscle cell culture. The results showed that the expression of nAChR on the muscle cell membrane was significantly increased after cells were exposed to dimethoate (130 microM). AChR function measured by carbachol-induced (22)Na+ influx demonstrated that dimethoate may inhibit the nAChR function either by binding to a noncompetitive site and changing the conformational state of nAChR or by blocking the nAChR channel directly. This study also demonstrated that dimethoate could rapidly induce the expression of c-fos, with a maximal effect at about 40 min, and c-fos might act as a transcriptional factor in regulating the expression of nAChR in the primary skeletal muscle cell culture following organophosphate exposure.
Yang
D
D
Center for Research on Occupational and Environmental Toxicology, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201, USA. yangdo@ohsu.edu
Lu
X
X
Zhang
W
W
He
F
F
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004117:Dimethoate / Q000633:toxicity*; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011978:Receptors, Nicotinic / Q000378:metabolism*
D007306:Insecticides; D011978:Receptors, Nicotinic; D004117:Dimethoate