title: Use of surface-modified capillaries in the separation and characterization of metallothionein isoforms by capillary electrophoresis inductively coupled plasma mass spectrometry.
abstract: The separation of metallothionein (ML) isoforms using capillary electrophoresis (CE) has been improved by applying surface-modified capillaries, and the metal composition of MTs has been characterized by subsequent inductively coupled plasma sector field mass spectrometry (ICPSFMS). Nine MT complexes in a commercial preparation from rabbit liver were successfully separated on an anionic polymer-coated column, prepared by immobilizing poly(2-acrylamido-2-methyl-1-propanesulfonic acid) on the fused-silica surface via a linking agent. On uncoated capillaries or those coated dynamically with cationic materials, only three complexes could be separated. Online isotope dilution analysis combined with CE/ICPSFMS indicated the stoichiometric molar metal contents in the MT complexes.
authors.affiliation: GKSS Research Center, Institute for Coastal Research/Physical and Chemical Analysis, Geesthacht, Germany.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Wang
authors.forename: Andreas
authors.initials: A
authors.lastname: Prange
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D013058:Mass Spectrometry; D008668:Metallothionein / Q000032:analysis*; D011108:Polymers; D020033:Protein Isoforms / Q000032:analysis; D011817:Rabbits; D013499:Surface Properties
chemical_list: D011108:Polymers; D020033:Protein Isoforms; D008668:Metallothionein