What is the value added by human intervention in protein structure prediction?
This article presents results of blind predictions submitted to the CASP4 protein structure prediction experiment. We made two sets of predictions: one using the fully automated SAM-T99 server and one using the improved SAM-T2K method with human intervention. Both methods use iterative hidden Markov model-based methods for constructing protein family profiles, using only sequence information. Although the SAM-T99 method is purely sequence based, the SAM-T2K method uses the predicted secondary structure of the target sequence and the known secondary structure of the templates to improve fold recognition and alignment. In this article, we try to determine what aspects of the SAM-T2K method were responsible for its significantly better performance in the CASP4 experiment in the hopes of producing a better automatic prediction server. The use of secondary structure prediction seems to be the most valuable single improvement, though the combined total of various human interventions is probably at least as important.
Karplus
K
K
Computer Engineering Department, University of California, Santa Cruz, 95064, USA. karplus@soe.ucsc.edu
Karchin
R
R
Barrett
C
C
Tu
S
S
Cline
M
M
Diekhans
M
M
Grate
L
L
Casper
J
J
Hughey
R
R
D000251:Adenosine Triphosphatases / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D003198:Computer Simulation; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry; D008190:Lyases / Q000737:chemistry; D008958:Models, Molecular; D051719:MutS DNA Mismatch-Binding Protein; D016571:Neural Networks, Computer; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000737:chemistry; D012107:Research Design; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C057119:FadR protein, Bacteria; D012097:Repressor Proteins; D004706:Endodeoxyribonucleases; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli; D008190:Lyases; C086788:magnesium chelatase