title: [Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs].
abstract: OBJECTIVE
  To evaluate the effect of autogenous periodontal ligament cell (PDL) transplantation in periodontal tissue regeneration.
  
  
  METHODS
  PDL cells derived from the same dog were cultured with alpha-MEM. 1 x 10(5) cells of first passage were allowed to attach to the collagen membrane for 24 hours. The membrane-cells were transplanted into periodontal defect in the same dog. Then the defects were covered with e-PTFE membranes. The defects covered only with e-PTFE were the controls. Eighteen teeth of 6 dogs for each group were included. The dogs were sacrificed after 6 weeks.
  
  
  RESULTS
  The results showed that new bone formation (4.00 +/- 0.13) mm in test group was significantly higher than that of in control group (3.09 +/- 0.28) mm, P < 0.05. The new cementum formation in test group was better than control group (P < 0.05).
  
  
  CONCLUSIONS
  The results suggested that PDL cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs.
authors.affiliation: Department of Stomatology, General Hospital of PLA, Beijing 100853, China.
authors.forename: L
authors.initials: L
authors.lastname: Ou
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Pang
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal; D008297:Male; D008567:Membranes, Artificial; D010513:Periodontal Ligament / Q000166:cytology*; D014182:Transplantation, Autologous
chemical_list: D008567:Membranes, Artificial