title: [Relationship between PDGF-A, bFGF and proliferation of smooth muscle cells in vein grafts].
abstract: OBJECTIVE
  To detect the relationship between platelet-derived growth factor-A (PDGF-A), basic fibroblast growth factor (bFGF), and proliferation of smooth muscle cells in vein grafts.
  
  
  METHODS
  An animal model of autogenous vein graft was established by transplanting internal branch of the jugular vein to the common iliac artery by end-to-end anastomosis. 25 rats were used. The grafted veins were harvested at 6 h, 2 d, 1 w, 2 w and 4 w respectively after the operation. The expression of PDGF-A and bFGF in different stages after grafting procedure was observed immunohistochemically.
  
  
  RESULTS
  The expression of PDGF-A peaked at 1 w, paralleled with proliferation of SMC, and was significantly higher than that at 6 h (P < 0.01). The expression of PDGF-A diminished gradually. At 4 w, it was significantly lower than that at 1 w (P < 0.01). bFGF demonstrated bimodal pattern of the expression: the first peak of expression occurred by 2 d and the second peak by 2 w. The difference was significantly compared with other stages.
  
  
  CONCLUSIONS
  The expression of PDGF-A 1 contributed to migration and proliferation of SMC. Except for contributing to proliferation of SMC, bFGF perhaps took part in endothelization.
authors.affiliation: Department of General Surgery, First Clinical College, China Medical University, Shenyang 110001.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Duan
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Xin
authors.forename: C
authors.initials: C
authors.lastname: Wei
authors.forename: C
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004730:Endothelium, Vascular / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007083:Iliac Artery / Q000601:surgery; D007601:Jugular Veins / Q000166:cytology / Q000637:transplantation*; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats; D014182:Transplantation, Autologous
chemical_list: D010982:Platelet-Derived Growth Factor; D016222:Fibroblast Growth Factor 2