High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products.
A HPLC procedure with a silica gel RP-18 reversed-phase column for the determination of aristolochic acids I, II in medicinal plants and slimming products was developed. The mobile system 0.3% ammonium carbonate solution-acetonitrile (75:25, v/v) with pH 7.5 was the optimal buffer to clearly separate aristolochic acids I, II within 20 min. The recovery of aristolochic acids I, II in medicinal plants and slimming products was better than 90% by extracting with methanol and purifying through a PHP-LH-20 column. The major component was aristolochic acid I in Aristolochia fangchi and the level ranged from 437 to 668 ppm. Aristolochic acid II was the major component for Aristolochia contorta and its range was <1-115 ppm. Twelve out of 16 samples of slimming pills and powders contained aristolochic acids I and/or II. The major component in most slimming products was aristolochic acid II and the level ranged from <1 to 148 ppm. It may indicate that slimming products were not mainly made of A. fangchi.
Lee
Tshung-Yei
TY
Department of Food Science, National Taiwan Ocean Univeristy, Keelung.
Wu
Ming-Lin
ML
Deng
Jou-Fang
JF
Hwang
Deng-Fwu
DF
D001067:Appetite Depressants / Q000737:chemistry*; D034341:Aristolochic Acids; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010616:Phenanthrenes / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D012680:Sensitivity and Specificity
D001067:Appetite Depressants; D034341:Aristolochic Acids; D010616:Phenanthrenes; C000228:aristolochic acid I