Inactivation of infectious salmon anaemia virus, viral haemorrhagic septicaemia virus and infectious pancreatic necrosis virus in water using UVC irradiation.
The UVC irradiation doses necessary for a 99.9% (3-log) inactivation of 3 different fish pathogenic viruses diluted in freshwater/seawater and wastewater from a fish processing plant were determined. The results showed that both infectious salmon anaemia virus (ISAV) and viral haemorrhagic septicaemia virus (VHSV) were very sensitive to UVC irradiation, showing a 3-log reduction of infectivity in freshwater of 33 +/- 3.5 and 7.9 +/- 1.5 J m(-2), respectively, while that of infectious pancreatic necrosis virus (IPNV) was substantially higher, 1188 +/- 57 J m(-2). Using ISAV as a model, a comparison of the effect of UVC irradiation on virus isolation versus reverse transcription polymerase chain reaction (RT-PCR) showed that considerably higher UVC doses, depending on the length of the amplified product, were necessary to abolish RT-PCR detection of viral RNA.
Oye
A K
AK
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian School of Veterinary Science, Oslo.
Rimstad
E
E
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005393:Fish Diseases / Q000517:prevention & control* / Q000821:virology; D005398:Fisheries; D005399:Fishes; D005618:Fresh Water / Q000821:virology; D018061:Infectious pancreatic necrosis virus / Q000528:radiation effects*; D029141:Novirhabdovirus / Q000528:radiation effects*; D009975:Orthomyxoviridae / Q000528:radiation effects*; D012367:RNA, Viral / Q000528:radiation effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012623:Seawater / Q000821:virology; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays; D014871:Water Microbiology
D012367:RNA, Viral