Determination of liposome/water partition coefficients of organic acids and bases by solid-phase microextraction.
The extraction of two methylated anilines and three chlorinated phenols by solid-phase microextraction (SPME) fibers coated with polyacrylate was investigated as a function of pH. Only the neutral species of the acids and bases partitioned into the polymer. Extraction kinetics were accelerated for the hydrophobic phenols at pH values around their acidity constant. This is presumably due to a reconstitution of the neutral species in the unstirred aqueous layer adjacent to the polymer surface by the charged species through the fast acid-base equilibrium. Although the charged species is not taken up into the polymer, liposome/water distribution ratios could be measured up to a pH value, where 99% of the compounds were present as charged species. The partition coefficients of the neutral and charged species were extrapolated from the pH profiles of the liposome/water distribution ratios. The resulting values were slightly lower than those measured with equilibrium dialysis. The discrepancies are discussed with respect to differences in the experimental conditions and the possibility of matrix effects during SPME measurements.
Escher
Beate I
BI
Swiss Federal Institute for Environmental Science and Technology (EAWAG), Dübendorf. escher@eawag.ch
Berg
Michael
M
Mühlemann
Jürg
J
Schwarz
Martin A A
MA
Hermens
Joop L M
JL
Vaes
Wouter H J
WH
Schwarzenbach
René P
RP