Determination of fexofenadine in human plasma and urine by liquid chromatography-mass spectrometry.
A sensitive method was developed to determine fexofenadine in human plasma and urine by HPLC-electrospray mass spectrometry with MDL 026042 as internal standard. Extraction was carried out on C18 solid-phase extraction cartridges. The mobile phases used for HPLC were: (A) 12 mM ammonium acetate in water and (B) acetonitrile. Chromatographic separation was achieved on a LUNA CN column (10 cm x 2.0 mm I.D., particle size 3 microm) using a linear gradient from 40% B to 60% B in 10 min. The mass spectrometer was operated in the selected ion monitoring mode using the respective MH+ ions, m/z 502.3 for fexofenadine and m/z 530.3 for the internal standard. The limit of quantification achieved with this method was 0.5 ng/ml in plasma and 1.0 ng in 50 microl of urine. The method described was successfully applied to the determination of fexofenadine in human plasma and urine in pharmacokinetic studies.
Hofmann
Ute
U
Dr. Margarete Fischer-Bosch-Institut für Klinische Pharmakologie, Stuttgart, Germany. ute.hofmann@ikp-stuttgart.de
Seiler
Monika
M
Drescher
Siegfried
S
Fromm
Martin F
MF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D016593:Terfenadine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
D006634:Histamine H1 Antagonists; D016593:Terfenadine; C093230:fexofenadine