Optimizing scale-up fermentation processes.
There are many aims associated with the optimization of fermentation processes. Optimization is expected to increase the yield of the final product but the process must be compliant with good manufacturing practices, the available equipment and the expected final scale of operation. Dealing with genetically modified microorganisms that overproduce recombinant protein has the advantage that the vast majority of the processes use only three different species, namely Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris. Standard processes for each organism are described in textbooks and serve as a basis for the development of a tailored process. This article outlines the general philosophy that we have devised to ensure an efficient approach of scaling up fermentation processes for biopharmaceutical purposes, in a multidisciplinary environment.
Thiry
Michel
M
Eurogentec S.A., Parc scientifique du Sart Tilman, B-4102 Seraing, Belgium. m.thiry@eurogenetics.com
Cingolani
Doriano
D
D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D003470:Culture Media / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D025461:Feedback, Physiological; D005285:Fermentation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000502:physiology; D010461:Peptones / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D010461:Peptones; D011994:Recombinant Proteins; D005947:Glucose; D010100:Oxygen