title: Sol-gel encapsulation of lactate dehydrogenase for optical sensing of L-lactate.
abstract: Sol-gel encapsulation of lactate dehydrogenase and its cofactor can be employed as a disposable sensor for L-lactate. The sensor utilized the changes in absorbance or fluorescence from the reduced cofactor nicotinamide adenine dinucleotide (NADH) upon exposure to L-lactate. Although, problems such as diminished enzymatic activity and/or leaching of enzyme from the sol-gel matrix occurred, the sol-gel process is sufficiently mild to permit retention of enzymatic activity. The apparent activity of LDH in the sensor is at least 10% of that of the dissolved enzyme. The sensor has a linear dynamic range over the normal physiological L-lactate level and has a long-term storage stability of at least 3 weeks.
authors.affiliation: Department of Chemistry, National Chung Cheng University, Ming-Hsiung, 621, Chia-Yi, Taiwan, ROC.
authors.forename: Chia I
authors.initials: CI
authors.lastname: Li
authors.forename: Yi Hua
authors.initials: YH
authors.lastname: Lin
authors.forename: Cheng Ling
authors.initials: CL
authors.lastname: Shih
authors.forename: Jeng Pyng
authors.initials: JP
authors.lastname: Tsaur
authors.forename: Lai Kwan
authors.initials: LK
authors.lastname: Chau
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D005782:Gels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D019344:Lactic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D017640:Silicates / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D017640:Silicates; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase