Cell surface expression of a specific antigenic site on the catalytic subunit of (Na(+) + K(+))-ATPase.
Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na(+) + K(+))-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na(+) + K(+))-ATPase is expressed on the cellular membrane surface.
Myers
Allen C
AC
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21224, USA.
Bochner
Bruce S
BS
Tomaselli
Gordon F
GF
Fedarko
Neal
N
Hudson
Sherry A
SA
Rohde
Holly
H
Huang
Shau-Ku
SK
Xu
Kai Y
KY
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification / Q000378:metabolism; D000918:Antibody Specificity / Q000276:immunology; D020134:Catalytic Domain / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology*; D002522:Chlorocebus aethiops; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D007668:Kidney / Q000166:cytology / Q000201:enzymology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D021122:Protein Subunits; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D021122:Protein Subunits; D000254:Sodium-Potassium-Exchanging ATPase