[Effect of bFGF, IGF-I and their synergy on the mitogenesis of bovine lens epithelial cells].
OBJECTIVE
To assess the effect of basic fibroblast growth factor (bFGF), insulin like growth factor-I (IGF-I) and their synergy on the mitosis of bovine lens epithelial cells (BLECs).


METHODS
BLECs were cultured in vitro. The fourth passage cells were inoculated into 24 well tissue culture plates, treated with different concentrations of bFGF, IGF-I, and bFGF + 20 microg/L IGF-I for 20 hours, (3)H-thymidine was added for 16 hours, then the radioactivity was determined by liquid scintillation counting.


RESULTS
Either bFGF (1 - 100 microg/L) or IGF-I (20 - 100 microg/L) increases mitosis of BLEC significantly, and 20 microg/L IGF-I may increase bFGF induced mitogenesis.


CONCLUSIONS
Growth factor and their synergic effect may play an important role in lens epithelial cell proliferation.
Sun
Z
Z
Department of Ophthalmology, The Second Affiliated Hospital of Zhejiang Medical University, Hangzhou 310009.
Yao
K
K
Wu
R
R
Shentu
X
X
Xu
W
W
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects*
D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I