title: Two neolignans from Perilla frutescens and their inhibition of nitric oxide synthase and tumor necrosis factor-alpha expression in murine macrophage cell line RAW 264.7.
abstract: Two neolignans were isolated from leaves of Perilla frutescens (Labiatae) as inhibitors of nitric oxide syntheses (IC50 5.9 microM and 53.5 microM, respectively) and tumor necrosis factor-alpha in lipopolysaccharide-activated RAW 264.7 cells. Their structures were identified as 1beta,2alpha,3beta,4alpha-1,2-dimethyl-3,4-bis-(2,4,5-trimethoxyphenyl)-cyclobutane (magnosalin), and 1alpha,2beta,3beta,4alpha-1,2-dimethyl-3,4-bis-(2,4,5-trimethoxyphenyl)-cyclobutane (andamanicin), and their activities were confirmed as resulted from the suppressed expression of inducible nitric oxide synthase enzyme and from the secretion of tumor necrosis factor-alpha from activated macrophages.
authors.affiliation: College of Pharmacy, Sookmyung Women's University, 140-742, Seoul, Republic of Korea. ryuha@sdic.sookmyung.ac.kr
authors.forename: Jae-Ha
authors.initials: JH
authors.lastname: Ryu
authors.forename: Haeng Ja
authors.initials: HJ
authors.lastname: Son
authors.forename: Sook Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D003503:Cyclobutanes / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D019686:Lamiaceae / Q000737:chemistry*; D017705:Lignans / Q000302:isolation & purification* / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: C475782:1alpha,2beta,3beta,4alpha-1,2-dimethyl-3,4-bis-(2,4,5-trimethoxyphenyl)-cyclobutane; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003503:Cyclobutanes; D017705:Lignans; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C069674:magnosalin; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse