title: HIV-1: a case of RT67 deletion in a multi-treated non responder patient.
abstract: The early detection of mutations in the HIV-1 polymerase is a key point in the management of anti-retroviral therapy. While nucleotide substitutions and insertions have been well and frequently desribed in literature as linked to drug resistance, deletions have been rarely observed and desribed (ART67, Imamichi et al.). The aim of this study is to describe a case of deletion of three nucleotides in the RT gene (ART67) of a multi-treated HIV-1 infected patient. As this deletion has not been detected by the oligoprobe assay, the phenotyping test was used to support therapy but without an appreciable success in terms of viral load. Then a sequencing based genotyping system was used to analyse the viral polymerase and a novel deletion was found at codon 67 of RT gene.
authors.affiliation: Servizio di Virologia, Azienda Ospedaliera Pugliese Ciaccio, Catanzaro, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Masciari
authors.forename: L
authors.initials: L
authors.lastname: Cosco
authors.forename: M C
authors.initials: MC
authors.lastname: Diaco
authors.forename: Dora N
authors.initials: DN
authors.lastname: Della
authors.forename: T
authors.initials: T
authors.lastname: Ferraro
authors.forename: T
authors.initials: T
authors.lastname: Raimondi
authors.forename: B
authors.initials: B
authors.lastname: Ruperti
authors.forename: E
authors.initials: E
authors.lastname: Santandrea
mesh_terms: D000328:Adult; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D024882:Drug Resistance, Viral / Q000235:genetics*; D004359:Drug Therapy, Combination; D005260:Female; D015658:HIV Infections / Q000175:diagnosis / Q000188:drug therapy*; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D018894:Reverse Transcriptase Inhibitors / Q000627:therapeutic use; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D017211:Treatment Failure; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase