Mapping of Myxococcus xanthus social motility dsp mutations to the dif genes.
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes. Another dsp allele contains a mutation in the sglK gene. About 36.6 kb around the dsp-dif locus was sequenced and annotated, and 50% of the genes are novel.
Lancero
Hope
H
Molecular Biology Institute and School of Dentistry, University of California-Los Angeles, Los Angeles, CA 90095-1668, USA.
Brofft
Jennifer E
JE
Downard
John
J
Birren
Bruce W
BW
Nusbaum
Chad
C
Naylor
Jerome
J
Shi
Wenyuan
W
Shimkets
Lawrence J
LJ
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D017353:Gene Deletion; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009068:Movement; D016941:Myxococcus xanthus / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins