Reversible control of enzymatic transglycosylations in supercritical fluoroform using a lipid-coated beta-D-galactosidase.
The transgalactosylation rate catalyzed by the lipid-coated beta-d-galactosidase in supercritical fluoroform (scCHF3) can be reversibly controlled by changing temperature or pressure (reflecting polarity changes) without damaging enzymes.
Mori
Toshiaki
T
Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuda, Midori-ku, Yokohama 226-8501, Japan.
Li
Ming
M
Kobayashi
Atsushi
A
Okahata
Yoshio
Y
D005617:Chlorofluorocarbons, Methane / Q000737:chemistry*; D006031:Glycosylation; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D011312:Pressure; D012995:Solubility; D013696:Temperature; D001616:beta-Galactosidase / Q000737:chemistry*
D005617:Chlorofluorocarbons, Methane; D008055:Lipids; D001616:beta-Galactosidase; C009554:fluoroform