Inhibition of HIV-1 reverse transcriptase and HIV-1 replication by Calophyllum coumarins and xanthones.
As part of our continuing study on the Calophyllum species, a number of coumarins, xanthones and chromene acids from different Calophyllum species of Sri Lanka were tested for inhibitory activity against the HIV-1 and its virally-encoded reverse transcriptase (RT). These compounds were found to be inactive in both the HIV-1 RT and whole virus systems. In contrast, cordatolide A and B demonstrated IC(50) values of 19.3 and 11.7 microM, respectively, against HIV-1 replication in a novel green fluorescent protein (GFP)-based reporter cell assay (HOG.R5).
Dharmaratne
H R W
HR
1 Natural Products Programme, Institute of Fundamental Studies, Kandy, Sri Lanka. hrwd13@hotmail.com
Tan
G T
GT
Marasinghe
G P K
GP
Pezzuto
J M
JM
D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology; D029759:Calophyllum; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*; D014966:Xanthenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044004:Xanthones
D019380:Anti-HIV Agents; D003374:Coumarins; D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; D014966:Xanthenes; D044004:Xanthones; C009689:xanthone; C113860:cordatolide A; D054303:HIV Reverse Transcriptase