Comparative karyology of Brazilian vampire bats Desmodus rotundus and Diphylla ecaudata (Phyllostomidae, Chiroptera): banding patterns, base-specific fluorochromes and FISH of ribosomal genes.
This paper provides new data on chromosomes of Brazilian vampire bats Desmodus rotundus and Diphylla ecaudata. These species were analyzed by GTG, CBG- and CB-DAPI banding, AgNO3/CMA3 sequential staining, base-specific fluorochrome dyes and in situ hybridization with 18S rDNA probe. C-banding (CBG) revealed constitutive heterochromatin in the pericentromeric regions in all autosomes and the X and Y chromosomes appeared entirely heterochromatic in both species. CB-DAPI revealed a coincident banding pattern to that obtained by CBG. Triple staining CMA3/DA/DAPI revealed an R-banding and a weak G-banding pattern in the karyotypes. Sequential AgNO3/CMA3 staining showed a NOR located interstitially on the long arm of pair 8 in D. rotundus and on the short arm of pair 13 in D. ecaudata. FISH with a rDNA probe confirmed the location and number of NORs; a difference neither in intensity nor in size of hybridization signal was detected between homologues for both species.
Santos
N
N
Departamento de Genética/CCB, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, 50732-970, Brazil. nsantos@npd.ufpe.br
Fagundes
V
V
Yonenaga-Yassuda
Y
Y
De Souza
M J
MJ
D000818:Animals; D001938:Brazil; D002503:Centromere / Q000737:chemistry; D002685:Chiroptera / Q000235:genetics*; D002871:Chromosome Banding; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D020459:Genes, rRNA; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics; D012680:Sensitivity and Specificity; D016622:Silver Staining; D014960:X Chromosome; D014998:Y Chromosome
D005456:Fluorescent Dyes; D006570:Heterochromatin