title: A cell line with multinucleated giant cell formation established from a human giant cell tumor of tendon sheath--preliminary report.
abstract: We first established a cell line with unique giant cell formation properties from a human giant cell tumor of tendon sheath (GCTTS) arising in the right ankle of a 7-year-old girl. The specimen for cell culture taken from the tumor was heterotransplanted into the back of a BALB/c (nu/nu) nude mouse. An in-vitro cell line was established from a tumor that grew after this heterotransplantation. Only mononuclear cells were observed in the primary culture, and these remained constant in growth. Multinucleated giant cells appeared at passage 3 and were constantly observed thereafter. The fusion of mononuclear cells into giant cells was verified by light and phase-contrast microscopy. This cell line was confirmed to be derived from a human by karyotype analysis and DNA fingerprinting. The cell-doubling time was 150 h. This cell line should be useful for studies of the mechanism of multinucleation in giant cell tumors.
authors.affiliation: Department of Orthopaedic Surgery, Tohoku University School of Medicine, Seiryomachi, Aobaku, Sendai 980-8574, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hosaka
authors.forename: M
authors.initials: M
authors.lastname: Hatori
authors.forename: R A
authors.initials: RA
authors.lastname: Smith
authors.forename: S
authors.initials: S
authors.lastname: Kokubun
mesh_terms: D000818:Animals; D000842:Ankle; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D005870:Giant Cell Tumors / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009372:Neoplasms, Connective Tissue / Q000473:pathology*; D013710:Tendons; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000473:pathology*