title: Crystal structure of a novel carboxypeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.
abstract: The structure of Pyrococcus furiosus carboxypeptidase (PfuCP) has been determined to 2.2 A resolution using multiwavelength anomalous diffraction (MAD) methods. PfuCP represents the first structure of the new M32 family of carboxypeptidases. The overall structure is comprised of a homodimer. Each subunit is mostly helical with its most pronounced feature being a deep substrate binding groove. The active site lies at the bottom of this groove and contains an HEXXH motif that coordinates the metal ion required for catalysis. Surprisingly, the structure is similar to the recently reported rat neurolysin. Comparison of these structures as well as sequence analyses with other homologous proteins reveal several conserved residues. The roles for these conserved residues in the catalytic mechanism are inferred based on modeling and their location.
authors.affiliation: Department of Chemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Arndt
authors.forename: Bing
authors.initials: B
authors.lastname: Hao
authors.forename: Vijay
authors.initials: V
authors.lastname: Ramakrishnan
authors.forename: Timothy
authors.initials: T
authors.lastname: Cheng
authors.forename: Sunney I
authors.initials: SI
authors.lastname: Chan
authors.forename: Michael K
authors.initials: MK
authors.lastname: Chan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002268:Carboxypeptidases / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008666:Metalloendopeptidases / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D020027:Pyrococcus furiosus / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D002268:Carboxypeptidases; D008666:Metalloendopeptidases; C062316:neurolysin