De novo expression of calretinin in trimethyltin-induced degeneration of developing rat hippocampus.
In the model of trimethyltin (TMT)-induced neurodegeneration in developing rat hippocampus, calretinin (CR)-immunoreactive neurons are selectively spared and even more numerous than in controls. We investigated the possibility of an additional synthesis of CR using RT-PCR. The amount of CR mRNA increased significantly after TMT treatment. CR mRNA production after TMT treatment could hypothetically be regarded as a compensatory phenomenon in developing rats.
Businaro
Rita
R
Department of Cardiovascular Sciences, University La Sapienza, Rome, Italy.
Corvino
Valentina
V
Geloso
Maria Concetta
MC
De Santis
Elena
E
Fumagalli
Lorenzo
L
Michetti
Fabrizio
F
D000818:Animals; D064032:Calbindin 2; D002118:Calcium / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D006624:Hippocampus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009410:Nerve Degeneration / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009498:Neurotoxins / Q000633:toxicity*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis* / Q000235:genetics; D014298:Trimethyltin Compounds / Q000633:toxicity*
C576512:Calb2 protein, rat; D064032:Calbindin 2; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D014298:Trimethyltin Compounds; C046488:trimethyltin; D002118:Calcium