[Study of the relation between mutant gene in bile and hepatic metastasis of large intestinal carcinoma].
OBJECTIVE
To study the value of point mutant genes in the diagnosis of hepatic metastasis of large intestinal carcinoma.


METHODS
Techniques of PCR-SSCP and DNA sequencing were used to detect point mutations of p53 and k-ras genes in primary focus, hepatic metastatic focus, and bile.


RESULTS
50 cases of large intestinal cancer were detected. 38 cases (76.0%) had p53 and k-ras gene mutations. p53 gene mutations were detected in 28 cases (56.0%) and k-ras gene mutations in 16 cases (32.0%). The same point mutant genes in bile with primary focus were detected in 10 out of 12 patients with hepatic metastasis (83.3%).


CONCLUSIONS
The tumor cells of hepatic metastasis show the same clonal origin with primary focal cells that had same point mutant genes. Detection of the point mutant genes in bile is of value in the diagnosis of hepatic metastasis of large intestinal carcinoma.
Li
S
S
Department of General Surgery, Beijing Military Area General Hospital of People's Liberation Army, Beijing 100700.
Yu
B
B
An
P
P
Yuan
S
S
Liang
Z
Z
D001646:Bile / Q000737:chemistry*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D005260:Female; D016158:Genes, p53 / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D017354:Point Mutation; D016133:Polymerase Chain Reaction