title: Rapid suppression of protein degradation in skeletal muscle after oral feeding of leucine in rats.
abstract: A diet containing adequate amounts of protein rapidly suppresses myofibrillar protein degradation in rats and mice. This study determined whether dietary amino acids inhibit postprandial protein degradation in rat skeletal muscle. When rats fed on a 20% casein diet for 1 h after 18 h starvation, the rate of myofibrillar protein degradation measured by N(tau)-methylhistidine release from the isolated extensor digitorum longus muscle was significantly (p < 0.05) decreased at 4 h after refeeding. A diet containing an amino acid mixture which is the same composition as casein also reduced myofibrillar protein degradation at 4 h after refeeding (p < 0.05). An essential amino acid mixture (15.1%, corresponding to casein composition) and a leucine (2.9%) diets reduced the rate of myofibrillar protein degradation after refeeding (p < 0.05), whereas a protein free diet did not. Administration of leucine alone (0.135 g/100 g body weight) by a feeding tube induced a decrease in the rate of myofibrillar protein degradation at 2 h after administration (p < 0.05), whereas the serum insulin concentration was constant after leucine administration. These results suggested that leucine is one of regulating factors of myofibrillar protein degradation after refeeding of a protein diet.
authors.affiliation: Food and Health Science, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagasawa
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Kido
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Yoshizawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ito
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Nishizawa