title: Priming and activation of mouse macrophages by trehalose 6,6'-dicorynomycolate vesicles from Corynebacterium glutamicum.
abstract: Vesicles consisting of pure trehalose dicorynomycolate (TDCM), the corynebacterial analog of the most studied mycobacterial glycolipid 'cord factor', were isolated from Corynebacterium glutamicum cells by mild detergent treatment; these induced in vivo a macrophage priming similar to that obtained with mycobacterial-derived trehalose dimycolate. In vitro, both TDCM and bacterial lipopolysaccharide (LPS) induced in macrophages the production of nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), endotoxin tolerance, and were primed for an enhanced secondary NO response to LPS. Interferon-gamma pretreatment did not influence the LPS-induced TNF-alpha response, but considerably increased the TDCM-induced response.
authors.affiliation: Laboratoire des Biomembranes, Institute de Biophysique et Biochimie Moléculaire et cellulaire, Centre de La Recherche Scientifique, Orsay, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Chami
authors.forename: Karine
authors.initials: K
authors.lastname: Andréau
authors.forename: Anne
authors.initials: A
authors.lastname: Lemassu
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Petit
authors.forename: Christine
authors.initials: C
authors.lastname: Houssin
authors.forename: Virginie
authors.initials: V
authors.lastname: Puech
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bayan
authors.forename: Richard
authors.initials: R
authors.lastname: Chaby
authors.forename: Mamadou
authors.initials: M
authors.lastname: Daffé
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002478:Cells, Cultured; D003311:Cord Factors / Q000276:immunology* / Q000302:isolation & purification; D003352:Corynebacterium / Q000276:immunology*; D005260:Female; D008262:Macrophage Activation / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000276:immunology*; D051379:Mice
chemical_list: D000276:Adjuvants, Immunologic; D003311:Cord Factors; C043399:6,6'-dicorynomycolyl trehalose