Characterization of the petI and res operons of Acidithiobacillus ferrooxidans.
DNA sequence analysis and bioinformatic interpretations have identified two adjacent clusters of genes potentially involved in the formation of a bc1 complex and in the maturation of a cytochrome c-type protein in two strains (ATCC 19859 and ATCC 33020) of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans). Reverse transcriptase-PCR experiments suggest that the two clusters are organized as operons, and +1 start sites of transcription for the operons have been determined by primer extension experiments. Potential promoters have been identified. The presence of these operons lends support to a recent model of reverse electron flow and is consistent with previous reports of phenotypic switching in this bacterium.
Levicán
Gloria
G
Laboratory of Bioinformatics and Molecular Microbiology, University of Santiago, 3363 Alameda, Santiago, Chile.
Bruscella
Patrice
P
Guacunano
Maritza
M
Inostroza
Carolina
C
Bonnefoy
Violaine
V
Holmes
David S
DS
Jedlicki
Eugenia
E
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003574:Cytochrome c Group / Q000235:genetics / Q000378:metabolism*; D014450:Electron Transport Complex III / Q000235:genetics / Q000378:metabolism*; D020563:Gammaproteobacteria / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D014450:Electron Transport Complex III