In vitro induction of bilirubin conjugation in primary rat hepatocyte culture.
UDP-glucuronosyltransferase (UGT1A1) is a critical enzyme in the elimination of bilirubin. The aim of our study was to investigate bilirubin conjugation in primary rat hepatocyte culture and the in vitro inducibility of this isoenzyme by inducing compounds of different classes: dexamethasone, clofibrate, rifampicin, and methylcholanthrene. Hepatocytes exhibited a marked decline in UGT1A1 activity in the first 4 h of culturing (10% of initial activity) and the recovery took 72 h. Immunoblot analysis proved that the loss of enzyme activity was associated with the decrease of protein concentration. Marked induction was detected in the cases of dexamethasone, clofibrate, and rifampicin treatments for 96 h both in enzyme activity (178, 176, and 168%) and in UGT1A1 protein level (362, 328, and 250%). The effects of dexamethasone and clofibrate were additive (210%). Methylcholanthrene had no influence on bilirubin conjugation in our system.
Jemnitz
Katalin
K
Institute of Chemistry Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary. jemnitz@chemres.hu
Lengyel
György
G
Vereczkey
László
L
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D002478:Cells, Cultured; D002994:Clofibrate / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D014453:Glucuronosyltransferase / Q000378:metabolism*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000960:Hypolipidemic Agents / Q000494:pharmacology; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012293:Rifampin / Q000494:pharmacology; D000099148:UGT1A1 Enzyme
D001663:Bilirubin; D002994:Clofibrate; D003907:Dexamethasone; D004791:Enzyme Inhibitors; D005938:Glucocorticoids; D014453:Glucuronosyltransferase; D000960:Hypolipidemic Agents; D007527:Isoenzymes; D008748:Methylcholanthrene; D012293:Rifampin; D000099148:UGT1A1 Enzyme; C499946:Ugt1a1 protein, rat