Expression of matrix metalloproteinase-2, tissue inhibitors of metalloproteinase-1, -3 at the implantation site of rhesus monkey during the early stage of pregnancy.
We have examined the expression of MMP-2, TIMP-1, and TIMP-3 mRNA at the implantation site of rhesus monkeys during early stage of pregnancy using in situ hybridization and Northern blot analysis. The results indicate that MMP-2 mRNA was mainly localized in the chorinoic villi and epithelial plaque, suggesting that MMP-2 may be involved in the process of epithelial plaque and trophoblast invasion. TIMP-3 was specifically expressed in the cells around the spiral arteries and maternal-fetal interface. Therefore, TIMP-3 may be the main inhibitor that restricts the trophoblast invasion. The TIMP-1 mRNA was detected in trophoblast villi and maternal decidua; however, its distribution was not cell-specific, suggesting a general role in the protection of trophoblast villi and maternal decidua from proteolysis by the MMPs secreted by themselves.
Gao
F
F
State Key Laboratory of Reproductive Biology, Chinese Academy of Sciences, Beijing, PR China.
Chen
X L
XL
Wei
P
P
Gao
H J
HJ
Liu
Y X
YX
D000818:Animals; D015152:Blotting, Northern; D003656:Decidua / Q000378:metabolism; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D008253:Macaca mulatta / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D009215:Myometrium / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism
D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019717:Tissue Inhibitor of Metalloproteinase-3; D020778:Matrix Metalloproteinase 2