Distribution of alpha-1 adrenoceptor subtypes in RNA and protein in rabbit eyes.
We investigated subtypes of alpha-1 adrenoceptor (AR) in rabbit ocular tissues using reverse transcription-polymerase chain reaction (RT - PCR), in situ hybridization (ISH) and binding studies. Competitive RT - PCR assays specific for the subtypes of alpha-1 AR revealed that the mRNA expression of alpha-1a AR was dominant, and that of each alpha-1b and alpha-1d was less than 10% and 0.5% of total alpha-1 ARs mRNA, respectively, in the iris, ciliary body, choroid and retina. In alpha-1a AR splice isoform-specific RT - PCR assays, we found a distinct proportion of each isoform mRNA in the iris, ciliary body and choroid. The results of the ISH assays for alpha-1a AR subtype showed that hybridization signals were clearly observed in the iris dilator muscle and in the epithelium of the ciliary processes. In binding studies, alpha-1A AR was a dominant subtype in the iris, choroid and retina in contrast to the ciliary body that had more alpha-1B than alpha-1A AR subtype at protein level.
Suzuki
Fumiko
F
Department of Pharmacology, School of Medicine, Fukui Medical University, Matsuoka, Fukui 910-1193 Japan.
Taniguchi
Takanobu
T
Nakamura
Seigo
S
Akagi
Yoshio
Y
Kubota
Chikara
C
Satoh
Makoto
M
Muramatsu
Ikunobu
I
D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D002829:Choroid / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005136:Eye Proteins / Q000378:metabolism*; D007498:Iris / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011224:Prazosin / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000317:Adrenergic alpha-Antagonists; D005136:Eye Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin