A gene-driven approach to the identification of ENU mutants in the mouse.
The construction of parallel archives of DNA and sperm from mice mutagenized with ethylnitrosurea (ENU) represents a potentially powerful and rapid approach for identifying point mutations in any gene in the mouse genome. We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program.
Coghill
Emma L
EL
MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell OX11 ORD, UK.
Hugill
Alison
A
Parkinson
Nick
N
Davison
Claire
C
Glenister
Peter
P
Clements
Sian
S
Hunter
Jackie
J
Cox
Roger D
RD
Brown
Steve D M
SD
D000483:Alleles; D000818:Animals; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D006579:Heterozygote; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation
D009153:Mutagens; D005038:Ethylnitrosourea