title: Synergistic effect of nisin and heat treatment on the growth of Escherichia coli O157:H7.
abstract: A combination of nisin and heat treatment was found to inhibit Escherichia coli O157:H7 effectively. After organisms were heated at 50, 52.5, and 55 degrees C for 5, 10, and 15 min, respectively, nisin was incorporated into the plates of E. coli O157:H7 at 0, 25, 50, and 100 IU/ml. The concentration of 100 IU/ml nisin significantly inhibited the growth of E. coli O157:H7 heated at 50 and 52.5 degrees C for 15 min. Nisin treatment at 100 IU/ml for 6 h resulted in the elimination of E. coli O157:H7 heated at 55 degrees C for 10 and 15 min.
authors.affiliation: Department of Environmental Health, Graduate School of Public Health, Seoul National University, Korea.
authors.forename: Jeong-In
authors.initials: JI
authors.lastname: Lee
authors.forename: Hu-Jang
authors.initials: HJ
authors.lastname: Lee
authors.forename: Mun-Han
authors.initials: MH
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D006358:Hot Temperature / Q000009:adverse effects*; D009561:Nisin / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D009561:Nisin