title: A rapid ELISA for the diagnosis of intravascular catheter related sepsis caused by coagulase negative staphylococci.
abstract: OBJECTIVE
  To develop and evaluate a rapid enzyme linked immunosorbent assay (ELISA) for the diagnosis of intravascular catheter related sepsis caused by coagulase negative staphylococci.
  
  
  METHODS
  Forty patients with a clinical and microbiological diagnosis of intravascular catheter related sepsis and positive blood cultures, caused by coagulase negative staphylococci, and 40 control patients requiring a central venous catheter as part of their clinical management were recruited into the study. Serum IgG responses to a previously undetected exocellular antigen produced by coagulase negative staphylococci, termed lipid S, were determined in the patient groups by a rapid ELISA.
  
  
  RESULTS
  There was a significant difference (p = < 0.0001) in serum IgG to lipid S between patients with catheter related sepsis and controls. The mean antibody titre in patients with sepsis caused by coagulase negative staphylococci was 10 429 (range, no detectable serum IgG antibody to 99 939), whereas serum IgG was not detected in the control group of patients.
  
  
  CONCLUSIONS
  The rapid ELISA offers a simple, economical, and rapid diagnostic test for suspected intravascular catheter related sepsis caused by coagulase negative staphylococci, which can be difficult to diagnose clinically. This may facilitate treatment with appropriate antimicrobials and may help prevent the unnecessary removal of intravascular catheters.
authors.affiliation: Department of Clinical Microbiology, University Hospital, Edgbaston, Birmingham B15 2TH, UK.
authors.forename: T
authors.initials: T
authors.lastname: Worthington
authors.forename: P A
authors.initials: PA
authors.lastname: Lambert
authors.forename: A
authors.initials: A
authors.lastname: Traube
authors.forename: T S J
authors.initials: TS
authors.lastname: Elliott
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D016470:Bacteremia / Q000175:diagnosis*; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D003030:Coagulase / Q000378:metabolism; D003428:Cross Infection / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004866:Equipment Contamination; D005260:Female; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D013203:Staphylococcal Infections / Q000175:diagnosis*; D013210:Staphylococcus / Q000201:enzymology / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D003030:Coagulase; D006017:Glycolipids; D007074:Immunoglobulin G; C420742:lipid S, Staphylococcus epidermidis