title: Peptidases affecting recombinant protein production by Streptomyces lividans.
abstract: The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.
authors.affiliation: Department of Chemical Engineering, University of Waterloo, ON, Canada.
authors.forename: S I
authors.initials: SI
authors.lastname: Yun
authors.forename: A R
authors.initials: AR
authors.lastname: Yahya
authors.forename: M
authors.initials: M
authors.lastname: Malten
authors.forename: D
authors.initials: D
authors.lastname: Cossar
authors.forename: W A
authors.initials: WA
authors.lastname: Anderson
authors.forename: J M
authors.initials: JM
authors.lastname: Scharer
authors.forename: M
authors.initials: M
authors.lastname: Moo-Young
mesh_terms: D000626:Aminopeptidases; D003470:Culture Media; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D010450:Endopeptidases / Q000494:pharmacology*; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013291:Streptococcus / Q000235:genetics*
chemical_list: D003470:Culture Media; D007377:Interleukin-3; D011994:Recombinant Proteins; D010450:Endopeptidases; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases