Rapid and sensitive method for the analysis of carbon monoxide in blood using gas chromatography with flame ionisation detection.
In order to measure changes in physiological CO concentrations in blood with good accuracy, a method was developed using gas chromatography with flame ionisation detection (250 degrees C). A nickel catalyst system was fitted to convert CO to methane at 375 degrees C after separation with a molecular sieve column at 35 degrees C. Helium was used as carrier at 30 ml/min. Porcine or human blood (400 microl) was sampled in gastight tubes and treated with sulfuric acid and saponin (800 microl). Accuracy was 1.4% and 1.5% (RSD), respectively. Precision was 2.8% (porcine blood). Limit of detection was 0.01 nmol/ml gas and limit of quantification 12 nmol/ml blood. Calibration was made in the interval 12-514 nmol/ml blood (corresponding to 0.1-6% COHb). Samples were stable for at least a month at +4 degrees C. This paper describes a method with high sensitivity and good accuracy, suitable for analysis of low CO concentrations.
Sundin
Anna-Maja
AM
Department of Surgery and Perioperative Sciences, Umea University Hospital, Sweden. annamaja.sundin@anestesi.umu.se
Larsson
Jan Erik
JE
D000818:Animals; D002248:Carbon Monoxide / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
D002248:Carbon Monoxide