title: A mutational analysis of the active site of human type II inosine 5'-monophosphate dehydrogenase.
abstract: The oxidation of IMP to XMP is the rate-limiting step in the de novo synthesis of guanine ribonucleotides. This NAD-dependent reaction is catalyzed by the enzyme inosine monophosphate dehydrogenase (IMPDH). Based upon the recent structural determination of IMPDH complexed to oxidized IMP (XMP*) and the potent uncompetitive inhibitor mycophenolic acid (MPA), we have selected active site residues and prepared mutants of human type II IMPDH. The catalytic parameters of these mutants were determined. Mutations G326A, D364A, and the active site nucleophile C331A all abolish enzyme activity to less than 0.1% of wild type. These residues line the IMP binding pocket and are necessary for correct positioning of the substrate, Asp364 serving to anchor the ribose ring of the nucleotide. In the MPA/NAD binding site, significant loss of activity was seen by mutation of any residue of the triad Arg322, Asn303, Asp274 which form a hydrogen bonding network lining one side of this pocket. From a model of NAD bound to the active site consistent with the mutational data, we propose that these resides are important in binding the ribose ring of the nicotinamide substrate. Additionally, mutations in the pair Thr333, Gln441, which lies close to the xanthine ring, cause a significant drop in the catalytic activity of IMPDH. It is proposed that these residues serve to deliver the catalytic water molecule required for hydrolysis of the cysteine-bound XMP* intermediate formed after oxidation by NAD.
authors.affiliation: Vertex Pharmaceuticals, Inc., 130 Waverly Street, Cambridge, MA 02139-4242, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Futer
authors.forename: Michael D
authors.initials: MD
authors.lastname: Sintchak
authors.forename: Paul R
authors.initials: PR
authors.lastname: Caron
authors.forename: Elmar
authors.initials: E
authors.lastname: Nimmesgern
authors.forename: Maureen T
authors.initials: MT
authors.lastname: DeCenzo
authors.forename: David J
authors.initials: DJ
authors.lastname: Livingston
authors.forename: Scott A
authors.initials: SA
authors.lastname: Raybuck
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D006868:Hydrolysis; D007168:IMP Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D007291:Inosine Monophosphate / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D009243:NAD / Q000737:chemistry; D012265:Ribonucleotides / Q000737:chemistry; D019820:Xanthine
chemical_list: D012265:Ribonucleotides; D009243:NAD; D007291:Inosine Monophosphate; D019820:Xanthine; C011141:xanthosine monophosphate; D007168:IMP Dehydrogenase