Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes.
The identification of two Arabidopsis thaliana genes involved in determining recessive resistance to several strains of the causal agent of bacterial wilt, Ralstonia solanacearum, is reported. Dominant (RRS1-S) and recessive (RRS1-R) alleles from susceptible and resistant accessions encode highly similar predicted proteins differing in length and which present a novel structure combining domains found in plant Toll-IL-1 receptor-nucleotide binding site-leucin-rich repeat resistance proteins and a WRKY motif characteristic of some plant transcriptional factors. Although genetically defined as a recessive allele, RRS1-R behaves as a dominant resistance gene in transgenic plants. Sequence analysis of the RRS1 genes present in two homozygous intragenic recombinant lines indicates that several domains of RRS1-R are essential for its resistance function. Additionally, RRS1-R-mediated resistance is partially salicylic acid- and NDR1-dependent, suggesting the existence of similar signaling pathways to those controlled by resistance genes in specific resistance.
Deslandes
Laurent
L
Max-Planck-Institut fur Züchtungsforschung, Abteilung Biochemie, Carl-von-Linne-Weg 10, D-50829 Cologne, Germany.
Olivier
Jocelyne
J
Theulieres
Frederic
F
Hirsch
Judith
J
Feng
Dong Xin
DX
Bittner-Eddy
Peter
P
Beynon
Jim
J
Marco
Yves
Y
D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D005808:Genes, Recessive; D016946:Gram-Negative Aerobic Rods and Cocci / Q000472:pathogenicity*; D007113:Immunity, Innate / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D020156:Salicylic Acid / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014157:Transcription Factors / Q000502:physiology
D029681:Arabidopsis Proteins; C109369:NDR1 protein, Arabidopsis; D010940:Plant Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D020156:Salicylic Acid