[The effect of protein kinase C on the invasive behavior of human salivary adenoid cystic carcinoma in vitro].
OBJECTIVE
To explore the relationship between protein kinase C (PKC) and invasion/metastasis of salivary adenoid cystic carcinoma and to explore the possibility of treatment against invasion/metastasis of the tumor cell by PKC inhibitor.


METHODS
SACC-83 cell line cells were treated by endogenous PKC inhibitor BS-PKCI extracted from bovine spermatozoa and exogenous PKC inhibitor H(7), while the adhesion to basement membrane, movement (expressed by the cell numbers moving out from agarose drop and the furthest distance) and invasive capacity in vitro were observed.


RESULTS
(1) The two inhibitors suppressed significantly adhesion of the tumor cell to basement membrane (P < 0.05). (2) The two inhibitors suppressed significantly movement of the tumor cells (P < 0.05). (3) The two inhibitors suppressed significantly invasive capacity of the tumor cells in vitro (P < 0.01).


CONCLUSIONS
There was a close relationship between PKC and invasion/metastasis of salivary adenoid cystic carcinoma; the two PKC inhibitor suppressed significantly adhesion, movement and invasive capacity of SACC - 83 cell line cells in vitro.
Sun
C
C
Department of Oral and Maxillofacial Surgery, the First Hospital, China Medical University, Shenyang 110001, China.
Wang
Z
Z
Wang
Y
Y
D003528:Carcinoma, Adenoid Cystic / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D006801:Humans; D009361:Neoplasm Invasiveness; D011493:Protein Kinase C / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012468:Salivary Gland Neoplasms / Q000201:enzymology / Q000473:pathology*
D047428:Protein Kinase Inhibitors; D011493:Protein Kinase C