Region-specific ontogeny of alpha-2,6-sialyltransferase during normal and cortisone-induced maturation in mouse intestine.
Regional differences in the ontogeny of mouse intestinal alpha-2,6-sialyltransferase activities (alpha-2,6-ST) and the influence of cortisone acetate (CA) on this expression were determined. High ST activity and alpha-2,6-ST mRNA levels were detected in immature small and large intestine, with activity increasing distally from the duodenum. As the mice matured, ST activity (predominantly alpha-2,6-ST) in the small intestine decreased rapidly to adult levels by the fourth postnatal week. CA precociously accelerated this region-specific ontogenic decline. A similar decline of ST mRNA levels reflected ST activity in the small, but not the large, intestine. Small intestinal sialyl alpha-2,6-linked glycoconjugates displayed similar developmental and CA induced-precocious declines when probed using Sambucus nigra agglutinin (SNA) lectin. SNA labeling demonstrated age-dependent diminished sialyl alpha2,6 glycoconjugate expression in goblet cells in the small (but not large) intestine, but no such regional specificity was apparent in microvillus membrane. This suggests differential regulation of sialyl alpha-2,6 glycoconjugates in absorptive vs. globlet cells. These age-dependent and region-specific differences in sialyl alpha-2,6 glycoconjugates may be mediated in part by altered alpha-2,6-ST gene expression regulated by trophic factors such as glucocorticoids.
Dai
Dingwei
D
Shanghai Institute for Pediatric Research, Xinhua Hospital and Shanghai Second Medical University, Shanghai, People's Republic of China 200092.
Nanthakumar
N Nanda
NN
Savidge
Tor C
TC
Newburg
David S
DS
Walker
W Allan
WA
D000375:Aging; D000818:Animals; D000833:Animals, Suckling; D001212:Asialoglycoproteins / Q000378:metabolism; D002236:Carbohydrate Conformation; D003106:Colon / Q000201:enzymology; D003348:Cortisone / Q000494:pharmacology*; D004386:Duodenum / Q000201:enzymology; D060748:Fetuins; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006001:Glycoconjugates / Q000032:analysis; D007082:Ileum / Q000201:enzymology; D007422:Intestines / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development*; D007583:Jejunum / Q000201:enzymology; D037102:Lectins; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D037121:Plant Lectins; D012333:RNA, Messenger / Q000032:analysis; D054788:Ribosome Inactivating Proteins; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D000509:alpha-Fetoproteins / Q000378:metabolism
D001212:Asialoglycoproteins; D060748:Fetuins; D005456:Fluorescent Dyes; D006001:Glycoconjugates; D037102:Lectins; D037121:Plant Lectins; D012333:RNA, Messenger; C050600:Sambucus nigra lectins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012799:Sialyltransferases; D054788:Ribosome Inactivating Proteins; D019158:N-Acetylneuraminic Acid; D016650:Fluorescein-5-isothiocyanate; D003348:Cortisone