title: [PCR in detecting the correlation between infection of HBV and cholelithiasis].
abstract: OBJECTIVE
  To study the correlation between cholelithiasis and the infection of HBV.
  
  
  METHODS
  32 formalin-fixed and paraffin-embedded gallbladder samples of cholelithiasis patients and 20 gallbladder samples of non-cholelithiasis patients were investigated using the polymerase chain reaction-Ethidium bromide (PCR-EB) assay. The 52 patients were positive to HBV serologic markers.
  
  
  RESULTS
  The results showed that HBV-DNA was found in 13 gallbladder samples of 32 cholelithiasis patients (40.63%), significantly higher than that in 3 gallbladder samples of 20 non-cholelithiasis patients (15%).
  
  
  CONCLUSIONS
  The infection of HBV and the formation of cholelithiasis are correlated.
authors.affiliation: Department of Surgery, First Clinical Hospital, China Medical University, Shenyang 110001.
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: R
authors.initials: R
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.forename: K
authors.initials: K
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Tong
authors.forename: K
authors.initials: K
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D002769:Cholelithiasis / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005704:Gallbladder / Q000473:pathology / Q000821:virology; D006509:Hepatitis B / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral