[PCR in detecting the correlation between infection of HBV and cholelithiasis].
OBJECTIVE
To study the correlation between cholelithiasis and the infection of HBV.


METHODS
32 formalin-fixed and paraffin-embedded gallbladder samples of cholelithiasis patients and 20 gallbladder samples of non-cholelithiasis patients were investigated using the polymerase chain reaction-Ethidium bromide (PCR-EB) assay. The 52 patients were positive to HBV serologic markers.


RESULTS
The results showed that HBV-DNA was found in 13 gallbladder samples of 32 cholelithiasis patients (40.63%), significantly higher than that in 3 gallbladder samples of 20 non-cholelithiasis patients (15%).


CONCLUSIONS
The infection of HBV and the formation of cholelithiasis are correlated.
Liu
X
X
Department of Surgery, First Clinical Hospital, China Medical University, Shenyang 110001.
Guo
R
R
Tian
Y
Y
Guo
K
K
Tong
Y
Y
Yu
K
K
D000328:Adult; D000368:Aged; D002769:Cholelithiasis / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005704:Gallbladder / Q000473:pathology / Q000821:virology; D006509:Hepatitis B / Q000175:diagnosis* / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*
D004279:DNA, Viral