Population-based study of fecal shedding of Clostridium perfringens in broodmares and foals.
OBJECTIVE
To determine the percentage of broodmares and foals that shed Clostridium perfringens in their feces and classify the genotypes of those isolates.


METHODS
Prospective cross-sectional study.


METHODS
128 broodmares and their foals on 6 equine premises.


METHODS
Anaerobic and aerobic bacteriologic cultures were performed on feces collected 3 times from broodmares and foals. All isolates of C. perfringens were genotyped.


RESULTS
Clostridium perfringens was isolated from the feces of 90% of 3-day-old foals and 64% of foals at 8 to 12 hours of age. A lower percentage of broodmares and 1- to 2-month-old foals shed C. perfringens in their feces, compared with neonatal foals. Among samples with positive results, C. perfringens type A was the most common genotype identified (85%); C. perfringens type A with the beta2 toxin gene was identified in 12% of samples, C. perfringens type A with the enterotoxin gene was identified in 2.1% of samples, and C. perfringens type C was identified in < 1% of samples.


CONCLUSIONS
Clostridium perfringens was identified from the feces of all but 6 foals by 3 days of age and is likely part of the normal microflora of neonatal foals. Most isolates from broodmares and foals are C. perfringens type A; thus, the clinical relevance of culture results alone is questionable. Clostridium perfringens type C, which has been associated with neonatal enterocolitis, is rarely found in the feces of horses.
Tillotson
Kirsten
K
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Traub-Dargatz
Josie L
JL
Dickinson
Charles E
CE
Ellis
Robert P
RP
Morley
Paul S
PS
Hyatt
Doreene R
DR
Magnuson
Roberta J
RJ
Riddle
W Thomas
WT
Bolte
Denise
D
Salman
M D
MD
D000818:Animals; D000831:Animals, Newborn; D003015:Clostridium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003430:Cross-Sectional Studies; D004760:Enterocolitis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006734:Horse Diseases / Q000175:diagnosis / Q000382:microbiology*; D006736:Horses; D008297:Male; D011446:Prospective Studies; D012307:Risk Factors; D013997:Time Factors