[The experimental research of purification and character of cultured retinal ganglion cell].
OBJECTIVE
To establish a cell line and purification model of retinal ganglion cells (RGCs) in vitro.


METHODS
RGCs from Sprague Dawley neonatal rats (postnatal 1 - 3 days) were cultured in basal medium eagle (BME) basal medium. The growth regularity of RGCs in vitro was observed under phase-contrast microscope. RGCs were purified by Thy 1.1 with FITC antibody and detected under fluorescent microscope and phase-contrast microscope.


RESULTS
Higher density of retinal cells and tectal extract facilitate cultured RGCs to survive. The purification rate of retinal ganglion cells in the experiment arrived at 95 percent.


CONCLUSIONS
Cytokine and trophic factors from other cells in the retina and tectal extract can promote RGCs to survive, and they can be purified by Thy 1.1 antibody.
Wu
Y
Y
Zhongshan Ophthalmic Center, Sun Yat-sun University of Medical Sciences, Guangzhou 510060.
Ge
J
J
Qiu
P
P
Li
Y
Y
Lin
M
M
Guo
Y
Y
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000166:cytology*