Rosemary-stimulated reduction of DNA strand breaks and FPG-sensitive sites in mammalian cells treated with H2O2 or visible light-excited Methylene Blue.
In this study possible protective effects of rosemary against oxidative DNA damage induced by H2O2- and visible light-excited Methylene Blue in colon cancer cells CaCo-2 and hamster lung cells V79 were investigated. The level of DNA damage (DNA strand breaks) was measured using the classical and modified single cell gel electrophoresis, so-called comet assay. Our findings showed that an ethanol extract from rosemary reduced the genotoxic activity of both agents after a long-term (24 h; 0.3 microg/ml) or short-term (2 h; 30 microg/ml) pre-incubation of cells. We suggest that the extract of rosemary exhibits a protective effect against oxidative damage to DNA as a consequence of scavenging of both *OH radicals and singlet oxygen ((1)O2).
Slamenova
Darina
D
Department of Mutagenesis and Carcinogenesis, Cancer Research Institute of Slovak Academy of Sciences, Vlarska 7, 833 91 Bratislava, Slovak Republic. exonslam@savba.sk
Kuboskova
Katarijna
K
Horvathova
Eva
E
Robichova
Sona
S
D000818:Animals; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects*; D043382:DNA-Formamidopyrimidine Glycosylase; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008027:Light; D008751:Methylene Blue; D009699:N-Glycosyl Hydrolases; D016877:Oxidants / Q000633:toxicity*; D028321:Plant Preparations / Q000494:pharmacology*; D027542:Rosmarinus
D016877:Oxidants; D028321:Plant Preparations; D006861:Hydrogen Peroxide; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D008751:Methylene Blue