(+)-Alpha-viniferin, a stilbene trimer from Caragana chamlague, inhibits acetylcholinesterase.
In the course of screening natural products for anti-acetylcholinesterase (AChE) activity, we found that a total methanolic extract of the underground parts of Caragana chamlague (Leguminosae) had significant inhibition towards AChE. Bioactivity-guided fractionation of the total methanolic extract resulted in the isolation and identification of two active stilbene oligomers, (+)-alpha-viniferin (1) and kobophenol A (2). Both 1 and 2 inhibited AChE activity in a dose-dependent manner, and the IC50 values of 1 and 2 were 2.0 and 115.8 microM, respectively. The AChE inhibitory activity of 1 was specific, reversible and noncompetitive.
Sung
Sang Hyun
SH
College of Pharmacy, Seoul National University, Korea.
Kang
So Young
SY
Lee
Ki Yong
KY
Park
Mi Jung
MJ
Kim
Jeong Hun
JH
Park
Jong Hee
JH
Kim
Young Chul
YC
Kim
Jinwoong
J
Kim
Young Choong
YC
D000110:Acetylcholinesterase / Q000737:chemistry; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013267:Stilbenes / Q000302:isolation & purification / Q000494:pharmacology*
D001572:Benzofurans; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D013267:Stilbenes; C064176:alpha-viniferin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase