Effect of hyperthermic preconditioning on the survival of ischemia-reperfused skin flaps: a new skin-flap model in the mouse.
To investigate whether hyperthermic preconditioning can actually protect skin flaps against ischemia/reperfusion injury, the authors first developed a new skin-flap model in 15 mice, a dorsal bipedicle island skin-flap model. Then, another 75 mice were separated into five groups. Mice in Groups 1 to 4 received the same hyperthermic preconditioning, but had different recovery times of 6 hr, 24 hr, 48 hr, and 72 hr, respectively. Mice in Group 5 served as control. Island skin flaps were elevated in all groups, and then were subjected to 8 hr of ischemia and subsequent reperfusion. Flap survival was statistically significantly higher than in controls in animals in Groups 1 and 3, with recovery times of 6 hr and 48 hr, respectively. Mice in Groups 2 and 4 had recovery times of 24 hr and 72 hr, respectively. Hyperthermic preconditioning could thus protect skin flaps against ischemia/reperfusion injury, and there were two optimal periods for such a protective effect.
Minh
Trinh Cao
TC
Department of Plastic and Reconstructive Surgery, University of Tokyo, Tokyo, Japan.
Ichioka
Shigeru
S
Nakatsuka
Takashi
T
Kawai
Junsuke
J
Shibata
Masahiro
M
Ando
Joji
J
Harii
Kiyonori
K
D000704:Analysis of Variance; D000818:Animals; D006085:Graft Survival; D006979:Hyperthermia, Induced; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015427:Reperfusion Injury / Q000517:prevention & control*; D012867:Skin / Q000098:blood supply*; D013524:Surgical Flaps