title: Mast cell chymase regulates dermal mast cell number in mice.
abstract: Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These results suggest that chymase released by mast cells may participate in local mast cell accumulation in a positive feedback fashion. Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor (SCF) on surface of the skin keratinocytes. In addition, incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium. Since soluble SCF is thought to regulate mast cell number, the chymase-induced mast cell accumulation may occur via the ability of chymase to process membrane-bound SCF on the epidermal keratinocytes.
authors.affiliation: Suntory Biomedical Research Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tomimori
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Muto
authors.forename: Harukazu
authors.initials: H
authors.lastname: Fukami
authors.forename: Kayo
authors.initials: K
authors.lastname: Saito
authors.forename: Chika
authors.initials: C
authors.lastname: Horikawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saito
authors.forename: Namino
authors.initials: N
authors.lastname: Sugiura
authors.forename: Motoo
authors.initials: M
authors.lastname: Sumida
authors.forename: Saki
authors.initials: S
authors.lastname: Kakutani
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D053818:Chymases; D003877:Dermatitis, Contact / Q000201:enzymology / Q000473:pathology; D020405:Dermis / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D006801:Humans; D007271:Injections, Intradermal; D015603:Keratinocytes / Q000201:enzymology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000008:administration & dosage / Q000502:physiology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012995:Solubility; D019089:Stem Cell Factor / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D019089:Stem Cell Factor; D004139:Dinitrofluorobenzene; D012697:Serine Endopeptidases; D053818:Chymases