[Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs].
OBJECTIVE
To evaluate the effect of autogenous periodontal ligament cell (PDL) transplantation in periodontal tissue regeneration.


METHODS
PDL cells derived from the same dog were cultured with alpha-MEM. 1 x 10(5) cells of first passage were allowed to attach to the collagen membrane for 24 hours. The membrane-cells were transplanted into periodontal defect in the same dog. Then the defects were covered with e-PTFE membranes. The defects covered only with e-PTFE were the controls. Eighteen teeth of 6 dogs for each group were included. The dogs were sacrificed after 6 weeks.


RESULTS
The results showed that new bone formation (4.00 +/- 0.13) mm in test group was significantly higher than that of in control group (3.09 +/- 0.28) mm, P < 0.05. The new cementum formation in test group was better than control group (P < 0.05).


CONCLUSIONS
The results suggested that PDL cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs.
Ou
L
L
Department of Stomatology, General Hospital of PLA, Beijing 100853, China.
Liu
H
H
Pang
J
J
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal; D008297:Male; D008567:Membranes, Artificial; D010513:Periodontal Ligament / Q000166:cytology*; D014182:Transplantation, Autologous
D008567:Membranes, Artificial