Soymilk products affect ethanol absorption and metabolism in rats during acute and chronic ethanol intake.
In this study we evaluated the effects of soy products on ethanol metabolism during periods of acute and chronic consumption in rats. Gastric ethanol content and blood ethanol and acetaldehyde concentrations were investigated after the oral administration of ethanol (34 mmol/kg) plus soy products such as soymilk (SM) or fermented soymilk (FSM). The gastric ethanol concentration of the FSM group was greater than that of the control group, whereas portal and aortal blood ethanol concentrations of the FSM group were lower than in controls. The aortal acetaldehyde concentration in the FSM group was lower than that of the control group. The direct effect of isoflavones on liver function was investigated by using hepatocytes isolated from untreated rats. Genistein (5 micromol/L) decreased ethanol (P = 0.045) and tended to decrease acetaldehyde (P = 0.10) concentrations in the culture filtrate. Some variables of ethanol metabolism in the liver were investigated after chronic ethanol exposure for 25 d. Rats consumed a 5% ethanol fluid plus the SM diet, the FSM diet or a control diet. Microsomal ethanol oxidizing activity was significantly lower in the FSM group than the control group. Furthermore, cytosolic glutathione S-transferase activity was higher in the SM and FSM groups than in the control group. Acetaldehyde dehydrogenase activity (low K(m)) in the FSM group (P = 0.15), but not in the SM group (P = 0.31), tended to be greater than in the control group. The amount of thiobarbituric acid reacting substances in the liver of the SM and FSM groups tended to be less than that of the control group (P = 0.18 and 0.10, respectively). These results demonstrate that soymilk products inhibit ethanol absorption and enhance ethanol metabolism in rats.
Kano
M
M
Yakult Central Institute for Microbiological Research, Yaho 1796, Kunitachi, Tokyo 186-8650, Japan. mitsuyoshi-kano@yakult.co.jp
Ishikawa
F
F
Matsubara
S
S
Kikuchi-Hayakawa
H
H
Shimakawa
Y
Y
D000042:Absorption; D000079:Acetaldehyde / Q000032:analysis*; D000284:Administration, Oral; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D005285:Fermentation; D019833:Genistein / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max; D013270:Stomach / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D007529:Isoflavones; D017392:Thiobarbituric Acid Reactive Substances; D000431:Ethanol; C004742:daidzein; D019833:Genistein; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+); D005982:Glutathione Transferase; D000079:Acetaldehyde