Identifying and modifying protein-DNA and protein-protein interactions using a bacterial two-hybrid selection system.
A bacterial two-hybrid system based on transcriptional activation in E. coli has recently been described. A variety of different protein-DNA and protein-protein interactions from bacteria, yeast, and humans have been studied using this bacterial-based system. The method, because it is based in bacteria, offers significant advantages relative to its yeast counterpart including the ability to analyze complex libraries > 10(8) in size, ease of use, and speed. The ability to easily and rapidly process very large libraries make this system a powerful tool for identifying, modifying, or optimizing protein-DNA and protein-protein interactions.
Joung
J K
JK
Department of Pathology, Division of Molecular Pathology and Research, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA. jjoung@partners.org
D000818:Animals; D050882:CREB-Binding Protein; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D005544:Forecasting; D015723:Gene Library; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D019151:Peptide Library; D015534:Trans-Activators; D004247:DNA; D050882:CREB-Binding Protein; C494363:CREBBP protein, human