title: Molecular characterization of frog chromogranin B reveals conservation of selective sequences encoding potential novel regulatory peptides.
abstract: Chromogranin B (CgB) is a member of the granin family of neuroendocrine secretory proteins, which has been proposed to play a role in secretory granule biogenesis and as a precursor to bioactive peptides. The cloning of CgB in a phylogenetically distant vertebrate, the frog Rana ridibunda, reveals a modest overall homology (35-40%) with mammalian CgB. However, the sequences of the N- and C-terminal regions are more highly conserved (57-65% amino acid identity) and may give rise to novel regulatory peptides. In frog, intense expression of CgB mRNA was observed in particular structures of the brain and in the distal lobe of the pituitary.
authors.affiliation: European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France.
authors.forename: Djida
authors.initials: D
authors.lastname: Aït-Ali
authors.forename: Valérie
authors.initials: V
authors.lastname: Turquier
authors.forename: David
authors.initials: D
authors.lastname: Alexandre
authors.forename: Luca
authors.initials: L
authors.lastname: Grumolato
authors.forename: Sylvie
authors.initials: S
authors.lastname: Jégou
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Youssef
authors.initials: Y
authors.lastname: Anouar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D053378:Chromogranin B; D002864:Chromogranins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D010455:Peptides; D012333:RNA, Messenger