Peptidases affecting recombinant protein production by Streptomyces lividans.
The influence of peptidases on human interleukin-3 (rhIL-3) production by a recombinant Streptomyces lividans strain was investigated. The bacterium produced several general peptidases and tripeptidyl peptidases compromising the authenticity of rhIL-3. The level of peptidases depended on growth morphology. Growing S. lividans as compact pellets successfully reduced peptidase activity. Maximum general peptidase activity in pellet culture was delayed after maximum rhIL-3 concentration was achieved. The activity of the tripeptidyl peptidase was product (rhIL-3) associated.
Yun
S I
SI
Department of Chemical Engineering, University of Waterloo, ON, Canada.
Yahya
A R
AR
Malten
M
M
Cossar
D
D
Anderson
W A
WA
Scharer
J M
JM
Moo-Young
M
M
D000626:Aminopeptidases; D003470:Culture Media; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D010450:Endopeptidases / Q000494:pharmacology*; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013291:Streptococcus / Q000235:genetics*
D003470:Culture Media; D007377:Interleukin-3; D011994:Recombinant Proteins; D010450:Endopeptidases; D000626:Aminopeptidases; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases