[The diagnostic value of perforin and granzyme B genes mRNA expression in acute rejection after hamster to rat orthotopic liver transplantation].
OBJECTIVE
To explore the mRNA expression of perforin and granzyme B genes activated by cytotoxic T lymphocytes (CTL) for monitoring early acute rejection after liver transplantation.


METHODS
The mRNA expression of perforin and granzyme B genes in grafts after gold hamster to rat liver transplantation was examined by reverse transcriptase polymerase chain reaction. Histological findings were studied comparatively, and isogenic rat liver transplantation was the control.


RESULTS
The mRNA expression of perforin and granzyme B genes began to be up-regulated on the day 3 after transplantation, when no histologic evidence of rejection was found. Levels of expression significantly increased after day 4 and persisted through the whole course of rejection. In contrast, the control group showed no expression of mRNA for perforin and granzyme B.


CONCLUSIONS
The determination of mRNA expression of perforin and granzyme B genes are useful to early diagnosis of acute rejection of liver transplantation.
Zhang
S
S
Orient Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438.
Wu
M
M
Tan
J
J
Yang
J
J
D000208:Acute Disease; D000818:Animals; D006224:Cricetinae; D042241:Early Diagnosis; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000175:diagnosis*; D053804:Granzymes; D016031:Liver Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008647:Mesocricetus; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D014183:Transplantation, Heterologous
D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes; C509215:Gzmb protein, rat; D012697:Serine Endopeptidases