Effects of pH and temperature on benzo[a]pyrene-DNA, -protein, and -lipid adducts in primary rat hepatocytes.
The effects of pH and temperature on benzo[a]pyrene(BaP)-DNA, -protein, and -lipid adducts were compared in rat hepatocytes treated with [3H]-BaP. In primary rat hepatocytes, BaP-DNA, -protein, and -lipid adducts were formed in a concentration-dependent manner (0.31-2.80 mmol BaP), and the formation of BaP-lipid adducts, specifically triglyceride (TG) adducts, correlated well with that of DNA or protein adducts. As incubation time increased (1-24 h), the formation of DNA or protein adducts was also elevated, while BaP-TG adducts decreased after 3 h of reaction. pH had no effect on the formation of [3H]-BaP-DNA, and -protein adducts, and the TG adducts decreased with increasing pH (3.4-11.4). With an increase in incubation temperature (30-45 degrees C), on the other hand, the adduct formation was increased with increasing temperature up to 40 degrees C and then decreased sharply. These results suggest that the physicochemical properties of BaP-DNA, -protein, and -TG adducts might be important to consider when cells or humans who have been exposed to BaP are monitored for biomarkers.
Park
Hyun Sun
HS
Division of Toxicology, School of Pharmacy, Sungkyunkwan University, Kyunggi-Do, Suwon, Korea.
Park
Yo An
YA
Lee
Byung Mu
BM
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D015415:Biomarkers; D002478:Cells, Cultured; D018736:DNA Adducts / Q000187:drug effects* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature
D015415:Biomarkers; D018736:DNA Adducts; D011506:Proteins; D001564:Benzo(a)pyrene