Linkage of boronated polylysine to glycoside moieties of polyclonal antibody; boronated antibodies as potential delivery agents for neutron capture therapy.
Among the ways to deliver comparatively large amounts of boron to cells in vitro for boron neutron capture studies is the linkage of a boronated macromolecule such as polylysine to an antibody. In order to reduce interference with immunoreactivity, boronated polylysine (BPL) was linked to oligosaccharide moieties on the IgG molecule distant from the antibody combining sites. The resultant bioconjugate was chromatographically separated from free BPL and unconjugated antibody using a Sephacryl S300 column. The total measured boron per BPL-IgG conjugate, determined by direct current plasma atomic emission spectroscopy, was estimated to be approximately 6 x 10(3) atoms. This, together with molecular weight estimations, indicated conjugation of about 3 polylysines to each IgG molecule. Immunoreactivity of the conjugate was found to be the same as that of the unconjugated polyclonal antibody. This was based on its concentration dependent interference with immunometric reactions for an antigen (TSH), whereas heat inactivated or non-specific antibody had no such inhibitory effects. The results support the hypothesis that the binding affinity of the conjugate for antigen was preserved after its linkage to BPL under the conditions described. The methodology described in this report may have applicability for the preparation of boronated antibodies as delivery agents for BNCT.
Novick
Sara
S
Institute of Nuclear Medicine, Soroka Medical Center, Ben Gurion University, Negev, Beer Sheva, Israel.
Quastel
Michael R
MR
Marcus
Stephanie
S
Chipman
David
D
Shani
Gad
G
Barth
Rolf F
RF
Soloway
Albert H
AH
D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D001896:Boron Compounds / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry; D005453:Fluorescence; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D011107:Polylysine / Q000737:chemistry; D011879:Radiotherapy Dosage
D000906:Antibodies; D001896:Boron Compounds; D002352:Carrier Proteins; D007074:Immunoglobulin G; D011107:Polylysine