Response times of carbon fiber microelectrodes to dynamic changes in catecholamine concentration.
The electrode response time and the measured concentrations during dynamic catecholamine changes were compared using constant potential amperometry and fast-scan cyclic voltammetry. The amperometric response to a rectangular pulse of catecholamine is more rectangular than the cyclic voltammetric response; however, the response times are very similar when, during cyclic voltammetry, the temporal lag due to adsorption and desorption of catecholamine to the electrode is removed by deconvolution. Deconvolution of cyclic voltammetry data was applied to stimulated dopamine release in vivo, allowing for modeling of release and uptake kinetics and to measure catecholamine release from single cells, resulting in better resolution of peaks from single vesicles. In vitro postcalibrations were performed to calculate concentrations of catecholamine measured with cyclic voltammetry and amperometry. The addition of 600 microM ascorbic acid to the postcalibration buffer, allowing a catalytic reaction to regenerate dopamine, resulted in similar calculated concentrations for stimulated release of dopamine using amperometry and cyclic voltammetry. Using deconvoluted cyclic voltammetry to remove the response time lag and adding ascorbic acid to the calibration buffer, the shape and concentration of dynamic catecholamine changes are very similar when measured with constant potential amperometry and cyclic voltammetry.
Venton
B Jill
BJ
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290, USA.
Troyer
Kevin P
KP
Wightman
R Mark
RM
D000818:Animals; D001923:Brain Chemistry; D002244:Carbon; D002395:Catecholamines / Q000032:analysis* / Q000378:metabolism; D002417:Cattle; D019439:Chromaffin Cells / Q000378:metabolism; D004298:Dopamine / Q000032:analysis / Q000378:metabolism; D008297:Male; D051379:Mice; D008839:Microelectrodes / Q000592:standards; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D002395:Catecholamines; D002244:Carbon; D004298:Dopamine