[Construction of linkage map of chromosomes 1 and 3 in large white x Meishan reference family].
A three-generation family of pigs has been constructed by using three Large White boars and seven sows of Meishan pigs as parents. In this family, five F1 males and twenty-three F1 females were intercrossed to generate 147 F2 offspring. According to the pig linkage map of USDA-MARC, eight and nine microsatellite markers selected on chromosomes 1 and 3 were chosen to span the entire chromosomes. The members of this family were genotyped. The characterization of these microsatellites was shown that they were polymorphic and could be used to construct linkage map and detect quantitative trait loci (QTLs). Linkage analyses were performed using the CRI-MAP software package. The lengths of the sex-averaged linkage map were 182.3 cM and 180.2 cM on chromosomes 1 and 3, respectively. There were some differences between the linkage maps in this study and of USDA-MARC. The linkage map of chromosome 1 in female was found to be shorter than in male, and the contrary was on chromosome 3.
Su
Yu-Hong
YH
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Xiong
Yuan-Zhu
YZ
Zhang
Qin
Q
Xia
Xuan-Yan
XY
Jiang
Si-Wen
SW
Deng
Chang-Yan
CY
Zheng
Rong
R
Lei
Ming-Gang
MG
Yang
Ze-Ming
ZM
D000818:Animals; D021461:Animals, Outbred Strains / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes; D018895:Microsatellite Repeats / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable; D013552:Swine / Q000235:genetics*