title: Ped3p is a peroxisomal ATP-binding cassette transporter that might supply substrates for fatty acid beta-oxidation.
abstract: Glyoxysomes, a group of specialized peroxisomes, are organelles that degrade fatty acids by the combination of fatty acid beta-oxidation and glyoxylate cycle. However, the mechanism underlying the transport of the fatty acids across the peroxisomal membrane is still obscure in higher plant cells. We identified and analyzed the PED3 gene and its gene product, Ped3p. The phenotype of the Arabidopsis ped3 mutant indicated that the mutation in the PED3 gene inhibits the activity of fatty acid beta-oxidation. Ped3p is a 149-kDa protein that exists in peroxisomal membranes. The amino acid sequence of Ped3p had a typical characteristic for "full-size" ATP-binding cassette (ABC) transporter consisting of two transmembrane regions and two ATP-binding regions. This protein was divided into two parts, that had 32% identical amino acid sequences. Each part showed a significant sequence similarity with peroxisomal "half" ABC transporters so far identified in mammals and yeast. Ped3p may contribute to the transport of fatty acids and their derivatives across the peroxisomal membrane.
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444-8585 Japan. makato@nibb.ac.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Nito
authors.forename: Rie
authors.initials: R
authors.lastname: Takei-Hoshi
authors.forename: Mina
authors.initials: M
authors.lastname: Yagi
authors.forename: Maki
authors.initials: M
authors.lastname: Kondo
authors.forename: Arata
authors.initials: A
authors.lastname: Suenaga
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Yamaya
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000251:Adenosine Triphosphatases; D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002874:Chromosome Mapping; D005227:Fatty Acids / Q000378:metabolism*; D006038:Glyoxylates / Q000378:metabolism; D020676:Glyoxysomes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000378:metabolism*; D010641:Phenotype; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014280:Triglycerides / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D029681:Arabidopsis Proteins; D005227:Fatty Acids; D006038:Glyoxylates; D014280:Triglycerides; D000251:Adenosine Triphosphatases; C456843:At4g39850 protein, Arabidopsis