[Changes of PMN apoptosis in rabbits after burn].
OBJECTIVE
To study the dynamic changes of apoptosis of PMN from rabbits in the early stage after severe burn in vivo and investigate the effect of burn serum and wound exudate on PMN apoptosis in vitro.


METHODS
12 rabbits were randomly divided into 2 groups: control group (37 degrees C) and burn group (95 degrees C, 15 sec, III degrees 30% TBSA). Whole blood from the burn group at 0, 4, 8, 12, 24 h postburn was stained with acridine orange/ethidium bromide (AO + EB), and observed under UV microscopy. PMNs were isolated from both groups and cultured for 24 h (37 degrees C, 5% CO(2)) with normal serum (NS), burn serum (BS), and subeschar tissue fluid (STF) respectively. Morphological assessment and quantitation of apoptosis were performed with AO + EB stain and flow cytometric analysis.


RESULTS
The number of apoptotic PMN in vivo decreased in the early stage postburn. In the BS + PMN group and STF + PMN group, the percentage of apoptotic PMN reduced (vs NS + PMN group, P < 0.05) and the specific DNA "ladder" pattern for apoptotic cells was not detected by agarose gel electrophoresis. We observed that both burn serum and STF could similarly suppressed PMN apoptosis while there was no significant difference between NS + normal PMN group and NS + burn PMN group.


CONCLUSIONS
Apoptosis is inhibited in PMN from rabbits postburn in the early stage. Both burn serum and STF from rabbits postburn can inhibit PMN apoptosis in vitro.
Zhang
P
P
Department of Burns, Xiangya Hospital, Hunan Medical University, Changsha 410078.
Ma
E
E
Huang
X
X
Luo
Z
Z
Zhang
S
S
D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000097:blood*; D004249:DNA Damage; D005122:Exudates and Transudates / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D011817:Rabbits; D011897:Random Allocation; D044967:Serum / Q000502:physiology