[Experimental study of the transferred keratinocytes transplanted on biomembrane].
OBJECTIVE
To search an ideal carrier of transferred keratinocytes for transplantation.


METHODS
The transferred keratinocytes were seeded on the surfaces of the artificial dermis and the silicone membrane and cultured in vitro for 2 weeks. The growth of the keratinocytes was observed by microscope and scanning electron microscope.


RESULTS
The keratinocytes implanted on the artificial dermis began to rupture and died after 2 to 3 days. While the keratinocytes adhered well on the surface of silicone membrane with pseudopodia formation after 1 week under scanning electron microscope, and the cells kept normal morphological and proliferative properties 2 weeks later.


CONCLUSIONS
The silicone membrane can be applied as an useful carrier for the keratinocytes transplantation.
Dong
Zhao-yang
ZY
Department of Burns and Plastic Surgery, Shanghai Corps Hospital, Chinese People's Armed Police Force, Shanghai, P. R. China 201103.
Chen
Yu-lin
YL
Kopp
Yeurgen
Y
Zhang
Wen-kui
WK
Yu
Jun-yi
JY
Wang
Lu
L
Fang
zhi-yang
zY
D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D008567:Membranes, Artificial; D012828:Silicones; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods
D008567:Membranes, Artificial; D012828:Silicones