title: Brown seaweed protein as an inhibitor of marine mollusk endo-(1-->3)-beta-D-glucanases.
abstract: Aqueous ethanol extracts from brown seaweed were found to contain substances inhibiting endo-(1-->3)-beta-D-glucanases, the digestive enzymes of marine mollusks. The inhibitors were detected in 70% of the brown seaweeds investigated. An irreversible protein inhibitor with high specificity for endo-(1-->3)-beta-D-glucanases of marine mollusks was isolated from the brown seaweed, Laminaria cichorioides. As determined by gel filtration, the molecular weight of the inhibitor was 46 kDa. The value of [I]50 (10(-8) M) for the inhibitor was comparable with the corresponding value for natural alpha-amylase inhibitors from terrestrial plants. Chemical modification results indicated that tryptophan, dicarboxylic acid, histidine and probably tyrosine residues of inhibitor molecule are important for interaction of the inhibitor with the enzyme.
authors.affiliation: Laboratory of Enzyme Chemistry, Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Prospect 100-Letiya 159, 690022, Vladivostok, Russia.
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Yermakova
authors.forename: Viktoriya V
authors.initials: VV
authors.lastname: Sova
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005936:Glucans; D007795:Laminaria / Q000737:chemistry; D008974:Mollusca / Q000201:enzymology*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011134:Polysaccharides / Q000378:metabolism; D012624:Seaweed / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D004791:Enzyme Inhibitors; D005936:Glucans; D010940:Plant Proteins; D011134:Polysaccharides; C008247:laminaran; D004693:Glucan Endo-1,3-beta-D-Glucosidase