Determination of amprenavir, a HIV-1 protease inhibitor, in human seminal plasma using high-performance liquid chromatography-tandem mass spectrometry.
A HPLC-MS-MS method to measure amprenavir in human seminal plasma has been developed and validated. The procedure uses stable, isotopically labeled 13C6-amprenavir as an internal standard and 100 microl of sample. The method is accurate (bias less than or equal to 7.2%) and precise (within- and between-day RSDs less than or equal to 4.2%) over the dynamic range of 30-4,000 ng/ml. Recently, this simple and sensitive method was used to determine amprenavir concentrations in seminal samples collected from HIV-1 positive subjects receiving amprenavir antiretroviral therapy as part of a multicenter clinical trial.
Pereira
Arlene S
AS
Department of Pathology, School of Medicine, University of North Carolina, Chapel Hill 27599, USA.
Kenney
Kathryn B
KB
Cohen
Myron S
MS
Eron
Joseph J
JJ
Tidwell
Richard R
RR
Dunn
John A
JA
D002138:Calibration; D002219:Carbamates; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005663:Furans; D017320:HIV Protease Inhibitors / Q000032:analysis*; D015497:HIV-1; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012661:Semen / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013449:Sulfonamides / Q000032:analysis*
D002219:Carbamates; D005663:Furans; D017320:HIV Protease Inhibitors; D013449:Sulfonamides; C095108:amprenavir