An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases.
A new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-microm, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.
Ceriotti
Laura
L
SAMLAB, Institute of Microtechnology, University of Neuchâtel, Switzerland. laura.ceriotti@unine.ch
de
RooijNicoF
R
Verpoorte
Elisabeth
E
D000596:Amino Acids / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes
D000596:Amino Acids; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate