Molecular characterization of Penicillium expansum MUCL mutants.
The modern biomolecular analysis of DNA was carried out to determine the identity of Penicillium expansum MUCL V1-V9 Variants with parental strain of Penicillium expansum MUCL 29412 and to compare the results with Penicillium verrucosum, a related species. The extracted DNAs were fragmented by digestion with restriction endonuclease Hind III and the fragments were separated by agarose gel electrophoresis. The DNAs were then denaturated in the gel after partial depurination with dilute acid and were transferred to a nylon membrane. The membrane was incubated with 32P-labeled probe, which was a DNA having a base sequence complementary to the DNA that was to be detected on the filter. The hybridization of the restriction fragments was performed for a highly qualitative comparison of the digested fragments. The analysis of the DNA profile, the most important stage in DNA identity testing, confirms the identity of the DNA for all strains of Penicillium expansum MUCL, except for V5 Variant.
Cercel
M
M
National Institute for Chemical-Pharmaceutical Research and Development (ICCF), 112 Calea Vitan, Bucharest 3, 74373, Romania.
Mouillard
C
C
Detertre
M
M
D018533:Biomass; D015139:Blotting, Southern; D004271:DNA, Fungal / Q000302:isolation & purification; D010407:Penicillium / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D004271:DNA, Fungal