title: Detection of Epstein-Barr virus and human T-cell lymphotropic virus type 1 in malignant nodal lymphoma, studied in Okinawa, a subtropical area in Japan.
abstract: Formalin-fixed paraffin-embedded lymph node samples were collected from 100 cases of malignant nodal lymphoma documented in Okinawa in the period from 1973 through 1998. According to the new World Health Organization classification, 12 cases were Hodgkin's lymphoma (HL). Eighty-eight cases of non-Hodgkin's lymphoma (NHL) included 54 cases of T-cell type and 34 cases of B-cell type. Using polymerase chain reaction (PCR), Epstein-Barr virus (EBV) was detected in 11 cases (91.7%) of HL and in 57 cases (64.8%) of NHL, and human T-cell lymphotropic virus type 1 (HTLV-1) was detected in 23 cases (26.1%) of NHL. Clonal integration of HTLV-1 was detected in 10 (43.5%) of 23 HTLV-1 PCR-positive cases by the inverse PCR technique. The EBV-infected cells were detected by EBER-1 in situ hybridization in 11 (91.7%) of 12 HL cases and in 64 (72.7%) of 88 NHL cases. Irrespective of phenotype and tissue type of the malignant lymphoma, the rate of EBV-positive infection in Okinawa was higher than that in any other districts reported in Japan. This characteristic high rate of EBV-positive infection in Okinawa can be ascribed to various factors, such as racial and geographical differences.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Miyagi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Toda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uezato
authors.forename: Kouichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyakuni
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takasu
authors.forename: Masato
authors.initials: M
authors.lastname: Masuda
mesh_terms: D002999:Clone Cells / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification; D020031:Epstein-Barr Virus Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D015490:HTLV-I Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D007564:Japan / Q000453:epidemiology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000453:epidemiology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology*; D008228:Lymphoma, Non-Hodgkin / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D014329:Tropical Climate; D016662:Virus Integration
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral