[The osteoinductivity and the dose-effect relationship with implantation of reconstituted bone xenograft: experimental study].
OBJECTIVE
To observe osteoinductivity and the dose-effect relationship with administration of reconstituted bone xenograft (RBX).


METHODS
A posterior thigh muscle pouch model with RBX transplantation was established in BALB/C mice. Samples were treated at regular postoperative intervals for radiologic, histomorphologic and ALP examinations.


RESULTS
(1) In the groups that had been implanted with RBX, the osteoinductivity was positively correlated with the bBMP contents in RBX (r = 0.7204, P < 0.01), showing a dose-dependent relationship; (2) satisfactory osteogenesis was noted in the RBX I group, whereas no substantial bone formation was seen in the group implanted with bBMP alone apart from some evidence of osteogenic effect; (3) the alkaline phosphatase activity attained its peak on the 7th day postoperatively and kept still higher to the 42nd day.


CONCLUSIONS
RBX is a highly osteoinductive grafting material with the treated homologous cancellous bone being a good slow-delivery carrier which can enhance the osteoinductive capacity of bBMP.
Zhao
C
C
Department of Orthopedics, 202nd Hospital, People's Liberation Army, Shenyang 110003.
Hu
Y
Y
Lu
R
R
Liu
J
J
Jin
G
G
Wang
Y
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000187:drug effects / Q000201:enzymology; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects*; D014183:Transplantation, Heterologous
D019485:Bone Morphogenetic Proteins; D000469:Alkaline Phosphatase