title: A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae.
abstract: A genotypic method for predicting rifampicin resistance in Mycobacterium leprae has been developed and rigorously tested on mouse footpad-derived and clinical specimens. A series of immobilized oligonucleotide capture probes can discriminate between wild type and mutant rpoB alleles, and positive controls are available for the most frequent mutation affecting Ser425. Two different non-radioactive detection formats have been tested with comparable success in both an industrialized and a developing country. The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients.
authors.affiliation: Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Honoré
authors.forename: P W
authors.initials: PW
authors.lastname: Roche
authors.forename: J H
authors.initials: JH
authors.lastname: Grosset
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
mesh_terms: D000818:Animals; D001483:Base Sequence; D004352:Drug Resistance, Microbial; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology*; D007918:Leprosy / Q000175:diagnosis* / Q000188:drug therapy; D051379:Mice; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011237:Predictive Value of Tests; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D007917:Leprostatic Agents; D015345:Oligonucleotide Probes; D012293:Rifampin