Developmental changes in the fine structure and histochemical properties of mucous cells in the parotid gland of the infant Japanese macaque.
Mucous cells have been known to occur in the terminal portions of the parotid gland in a few species of mammals during a limited period of their development. The aim of this study was to examine the occurrence and features of mucous cells in the parotid gland of the infant Japanese macaque. Light microscopy revealed that mucous cells in the macaque parotid gland were present in the terminal clusters and acini at postnatal day 15, were less prevalent at day 30, and continued to decrease in number over 3 months. Mucous cells were no longer recognized in the parotid gland in 6-month-old macaques. Electron microscopy showed that the mucous cells contained electron-lucent secretory granules and bipartite or tripartite secretory granules. By 3 months of age, there was a scarcity of mucous cells and a concomitant increase in transitional cells. These transitional cells were intermediate in structure between mucous and serous cells, and contained three types of granules: electron-lucent, bipartite or tripartite, and electron-dense. None of the cells showed apoptotic figures. Lectin histochemistry indicated that the mucous cells in the early postnatal period had sugar residues identical in nature to those seen in the granules from mature serous cells in the glands of 3-month-old macaques. Immunohistochemistry using an antibody against human alpha-amylase showed a weakly positive reactivity in the secretory granules of the mucous cells, starting from day 15. In the transitional cells, the electron-dense granules showed a stronger immunoreactivity than either the electron-lucent granules or the heterogeneously structured granules. These results suggest that the secretory granules of mucous cells have characteristics in common with those of serous cells, and that during the transitional period the mucous granules change from the initial electron-lucent to hetorogenous forms, finally becoming the electron-dense granules. The mucous cells in the parotid gland of the juvenile Japanese macaque are therefore suggested to be converted into serous cells.
Ikeda
R
R
Department of Histology, The Nippon Dental University School of Dentistry at Tokyo, Japan. ikedarie@tokyo.ndu.ac.jp
Aiyama
S
S
Tsujimura
T
T
Okamoto
K
K
D000818:Animals; D000831:Animals, Newborn; D005260:Female; D007150:Immunohistochemistry; D037102:Lectins; D008251:Macaca / Q000033:anatomy & histology*; D008297:Male; D008709:Methenamine; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009093:Mucus / Q000166:cytology* / Q000378:metabolism; D010306:Parotid Gland / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D022142:Secretory Vesicles / Q000648:ultrastructure
D037102:Lectins; D008709:Methenamine