[Mechanism of abnormal scars with treatment of steroid].
OBJECTIVE
To investigate the mechanism of steroid in treatment of abnormal scars.


METHODS
Apoptosis of different fibroblasts from 6 samples with keloid, 6 samples with hypertrophic scar, and 6 samples of normal skin was observed under the condition of the media containing steroid in vitro. Proliferation, biosynthesis and apoptosis of fibroblasts of 6 samples of hypertrophic scars treated with intralesional injection of steroid were studied in vivo.


RESULTS
Steroid could induce apoptosis of different fibroblasts in vitro in correspondence with increasing ratio of Bax/Bcl-2 proteins. Intralesional injection of steroid could inhibit proliferation of fibroblasts from hypertrophic scars by inhibiting PDGF-BB gene expression in vivo. Intralesional injection of steroid could inhibit procollagen gene expression to prohibit type I and III protein syntheses of fibroblasts from hypertrophic scars in vivo by inhibiting gene transcription. Intralesional injection of steroid could increase c-myc and p53 gene expression of hypertrophic scars in vivo, which induced apoptosis of cells.


CONCLUSIONS
The effects of steroid on abnormal scars were achieved by inhibiting proliferation and biosyntheses of fibroblasts and promoting apoptosis of fibroblasts.
Bao
W
W
Research Center of Plastic Surgery, Third Clinical School, Beijing Medical University, Beijing 100083, China.
Xu
S
S
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D000077214:Becaplermin; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002921:Cicatrix / Q000188:drug therapy* / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D020574:Proto-Oncogene Proteins c-sis; D013256:Steroids / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D010982:Platelet-Derived Growth Factor; D019253:Proto-Oncogene Proteins c-bcl-2; D016271:Proto-Oncogene Proteins c-myc; D020574:Proto-Oncogene Proteins c-sis; D013256:Steroids; D016159:Tumor Suppressor Protein p53; D000077214:Becaplermin; D003094:Collagen