IFN-gamma-producing CD45RA+CD8+ and IL-10-producing CD45RA-CD4+ T cells generated in response to LACK in naive subjects never exposed to Leishmania.
Leishmania guyanensis (L.g.)-specific CD8+ T cells can be isolated from PBMC of subjects who have never been previously exposed to Leishmania. Cells that produce IFN-gamma in response to live L.g. are generated from naive CD45RA+CD8+ T cells. The generation of L.g.-specific CD8+ T cells requires the presence of whole L.g. or UV-irradiated parasite but not the soluble antigens from L.g. promastigotes. The IFN-gamma-producing T cells recognize a specific antigen, the Leishmania homologue of receptors of activated C kinases (LACK) and this antigen but not live L.g. can produce a strong IL-10 response in CD45RA-CD4+ memory T cells from naive subjects. A single epitope (amino acid 156-173) is found to induce the IL-10 synthesis. While the IFN-gamma-producing cells are present among CD45RA+CD8+ T cells that are CD62L-CDR7- and CLA-, the LACK-reactive IL-10-producing cells are CD4+ T cells that are CD62L+CCR7- and CLA-.
Bourreau
Eliane
E
Immunologie des Leishmanioses, Institut Pasteur de la Guyane Française, BP 6010, 97304 Cayenne, French Guiana.
Collet
Marion
M
Prévot
Ghislaine
G
Milon
Geneviève
G
Ashimoff
Dominique
D
Hasagewa
Hitoshi
H
Parra-Lopez
Carlos
C
Launois
Pascal
P
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D018334:Leishmania guyanensis / Q000276:immunology* / Q000472:pathogenicity; D007897:Leishmaniasis, Mucocutaneous / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation; D010641:Phenotype; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000953:Antigens, Protozoan; D016207:Cytokines; D015800:Protozoan Proteins; D016753:Interleukin-10; C093139:LACK antigen, Leishmania; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens