Mast cell chymase regulates dermal mast cell number in mice.
Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These results suggest that chymase released by mast cells may participate in local mast cell accumulation in a positive feedback fashion. Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor (SCF) on surface of the skin keratinocytes. In addition, incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium. Since soluble SCF is thought to regulate mast cell number, the chymase-induced mast cell accumulation may occur via the ability of chymase to process membrane-bound SCF on the epidermal keratinocytes.
Tomimori
Yoshiaki
Y
Suntory Biomedical Research Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan.
Muto
Tsuyoshi
T
Fukami
Harukazu
H
Saito
Kayo
K
Horikawa
Chika
C
Tsuruoka
Nobuo
N
Yamashiro
Kyoko
K
Saito
Masayuki
M
Sugiura
Namino
N
Sumida
Motoo
M
Kakutani
Saki
S
Fukuda
Yoshiaki
Y
D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D053818:Chymases; D003877:Dermatitis, Contact / Q000201:enzymology / Q000473:pathology; D020405:Dermis / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D006801:Humans; D007271:Injections, Intradermal; D015603:Keratinocytes / Q000201:enzymology / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000008:administration & dosage / Q000502:physiology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012995:Solubility; D019089:Stem Cell Factor / Q000096:biosynthesis; D015854:Up-Regulation / Q000187:drug effects
D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; D019089:Stem Cell Factor; D004139:Dinitrofluorobenzene; D012697:Serine Endopeptidases; D053818:Chymases