Phage antibodies from combinatorial library neutralize vaccinia virus.
The library of human scFv antibodies displayed on the surface of bacteriophages was panned against Vaccinia virus (VACV), strain Elstree. 75% binding with Vaccinia virus. 5 clones were characterized for their binding with VACV and their ability to neutralize VACV in plaque reduction neutralization test (PRNT). Antibodies from the clones were obtained as soluble individual molecules and their binding activities were confirmed in ELISA.
Tikunova
N V
NV
Research Center of Virology and Biotechnology, Vector, Koltsovo, Novosibirsk region, 630559, Russia. tikunova@vector.nsk.su
Morozova
V V
VV
Batanova
T A
TA
Belanov
E F
EF
Bormotov
N I
NI
Ilyichev
A A
AA
D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D020650:Combinatorial Chemistry Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000302:isolation & purification; D019151:Peptide Library; D011994:Recombinant Proteins / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology*
D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D011994:Recombinant Proteins; C055966:immunoglobulin Fv