title: [The feature of pancreatic microcirculatory impairment in caerulein induced acute pancreatitis].
abstract: OBJECTIVE
  To study the feature of pancreatic microcirculatory impairment in caerulein-induced acute pancreatitis.
  
  
  METHODS
  The AP model of subcutaneous injection of caerulein was studied by intravital fluorescence microscopy of erythrocytes labeled by FITC (FITC-RBC) and by scanning electron microscopy of vascular corrosion casts and light microscopy of Chinese ink-injected/cleared tissues.
  
  
  RESULTS
  Animals treated with caerulein showed hyper-amylases. Contractions of intralobular arteriolar sphincter, presence of vacuoles in all the layers of sphincter, gross irregularity in capillary network of acini were observed in caerulein-induced groups. The decrease of pancreatic capillary blood flow (P < 0.01), reduction of functional capillary density, and irregular intermittent perfusion (P < 0.05) were found.
  
  
  CONCLUSIONS
  Impairment and contraction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis induced by Caerulein, and play a key role in the pancreatic ischemia and pancreatic microvascular failure in acute pancreatitis.
authors.affiliation: Department of Hepato-Bili-Pancreato-Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yan
authors.forename: Y
authors.initials: Y
authors.lastname: Shu
authors.forename: Z
authors.initials: Z
authors.lastname: Cheng
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: P
authors.initials: P
authors.lastname: Lan
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.forename: R
authors.initials: R
authors.lastname: Wang
mesh_terms: D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D004195:Disease Models, Animal; D007279:Injections, Subcutaneous; D008297:Male; D008833:Microcirculation; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002108:Ceruletide