title: Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors.
abstract: The effects of acute physiological stressors (5 s tail pinch, handling and forced swimming at +25 and +5 degrees C for 3 min each) on serotonin (5-HT) release in the mouse brain were investigated using in vivo microdialysis. The extracellular 5-HT levels were determined by a newly developed highly-sensitive and selective high-performance liquid chromatography method based on derivatization with benzylamine and fluorescence detection. The basal levels of 5-HT in 3 min microdialysates from the ventral hippocampus and frontal cortex were 0.68+/-0.21 and 0.75+/-0.28 fmol/6 microl (n=24), respectively. All three stressors caused an immediate, significant and reversible increase (handling: 150%; swimming: 240%) of extracellular 5-HT levels in both brain structures, suggesting a more dynamic role played by the serotonergic system in response to acute stress.
authors.affiliation: Chemicals Evaluation and Research Institute, 3-822 Ishii Machi, Hita, Oita 877-0061, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Fujino
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshitake
authors.forename: Osamu
authors.initials: O
authors.lastname: Inoue
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Ibii
authors.forename: Jan
authors.initials: J
authors.lastname: Kehr
authors.forename: Junichi
authors.initials: J
authors.lastname: Ishida
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nohta
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yamaguchi
mesh_terms: D000208:Acute Disease; D000818:Animals; D005110:Extracellular Space / Q000378:metabolism*; D005625:Frontal Lobe / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D017551:Microdialysis; D009474:Neurons / Q000378:metabolism*; D010146:Pain / Q000378:metabolism / Q000503:physiopathology; D010812:Physical Stimulation; D012149:Restraint, Physical; D012701:Serotonin / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D013550:Swimming; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D012701:Serotonin