title: Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma.
abstract: Viral respiratory tract infections are associated frequently with acute exacerbations of asthma. Nasopharyngeal aspirates and bronchoalveolar lavage specimens are used extensively for detecting viral respiratory tract infections, but not sputum. The aim of the study was to determine the efficiency of viral detection in induced sputum versus nasopharyngeal aspirate obtained during acute exacerbations of asthma, comparing three laboratory methods of viral diagnosis. Paired samples of induced sputum and nasopharyngeal aspirate obtained from 32 adults admitted to hospital with acute asthma were subjected to reverse transcription-polymerase chain reaction (RT-PCR), viral culture, and immunofluorescence assay. The results show that RT-PCR was associated with significantly higher rates of viral detection than culture (P=0.005) or immunofluorescence (P=0.001), without significant differences in the rates of viral detection between induced sputum and nasopharyngeal aspirate. It is concluded that induced sputum specimens are feasible for detection of viral respiratory tract infections by RT-PCR during acute exacerbations of asthma.
authors.affiliation: Department of Medicine, Respiratory Division, National University of Singapore, 119260, Singapore, Singapore.
authors.forename: Xueyu
authors.initials: X
authors.lastname: Xiang
authors.forename: Diwen
authors.initials: D
authors.lastname: Qiu
authors.forename: Kwai Peng
authors.initials: KP
authors.lastname: Chan
authors.forename: Soh Ha
authors.initials: SH
authors.lastname: Chan
authors.forename: Richard G
authors.initials: RG
authors.lastname: Hegele
authors.forename: Wan C
authors.initials: WC
authors.lastname: Tan
mesh_terms: D000208:Acute Disease; D000328:Adult; D001249:Asthma / Q000150:complications*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D009305:Nasopharynx / Q000821:virology*; D012327:RNA Virus Infections / Q000150:complications / Q000175:diagnosis*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D012141:Respiratory Tract Infections / Q000150:complications / Q000175:diagnosis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013183:Sputum / Q000821:virology*
chemical_list: D012367:RNA, Viral