[Biological behavior of 3 strains of the dengue-2 virus in 2 cell lines of mosquitoes].
Strains A-15 (isolated in Cuba, 1981), Jamaica (isolated in Jamaica, 1981) and Nueva Guinea "C" (standard) from dengue-2 virus were compared according to the time of appearance of the cytopathic effect (CPE), to the time of appearance of specific fluorescence and to the kynetics of viral multiplication on being innoculated in the cell lines AP-61 (Aedes pseudoscutellaris) and C6/36 HT (Aedes albopictus). The results showed that the CPE of highest intensity and earliest appearance was for A-15, followed by Jamaica and Nueva Guinea "C" (NGC). AP-61 seems to favor the CPE of Jamaica with respect to that of the same strain in C6/36 HT. The fluorescence was earlier for Jamaica and A-15 and more intensive for the latter, whereas NGC manifested late. This behaviour was similar in the 2 cellular systems. The greatest titres during the kinetics of viral multiplication were obtained from A-15 in both lines, although in AP-61 they tend to be equal from the 4th day on. The strain A-15 showed a particular behaviour of these biological properties on comparing them with the other strains under study, which may be related to changes found in its neucleotide sequence.
Morier
L
L
Laboratorio de referencia y diagnóstico de dengue y otras enfermedades virales (LARDIDEV), Maracay, Venezuela. ciipk@ipk.sld.cu
Camacho
D E
DE
Guzmán
M G
MG
Alvarez
M
M
Rodríguez
R
R
Comach
G
G
D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line / Q000821:virology; D003716:Dengue Virus / Q000145:classification* / Q000502:physiology*