[Morphometrical study on inhibitory effect of vitamin B6 and banana-stem extract on calcium crystallization].
OBJECTIVE
To search for the anticrystallization mechanism by vitamin B(6) and banana stem extract.


METHODS
Thirty-six male China-1 mice were divided into 4 groups: normal, crystallization, vitamin B(6) and banana-stem extract groups. Calcium oxalate crystallization was induced by 1% glycol and 1% ammonium chloride in the last 3 groups. Vitamin B(6) was then given to the 3rd group and banana stem extract to the 4th group for 3 weeks. At the end of 3 weeks, all the mice were sacrificed and the kidneys were taken for morphometrical studies using image analysis system and assayed for calcium oxalate.


RESULTS
Morphometrical area density and numerical density of crystallization was significantly less in the vitamin B(6) group and the banana-stem extract group as compared with the crystallization group, being the least in the banana-stem extract group. Oxalate in renal tissue was also significantly reduced. Both vitamin B(6) and banana stem extract had no effect on renal tissue calcium.


CONCLUSIONS
Vitamin B(6) and banana-stem extract may be useful agents in the treatment of patients with hyperocaluric urolithiasis.
Li
S
S
Department of Pathology, Guangxi Medical University, Nanning 530021.
Wu
C
C
Nong
H
H
Deng
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002129:Calcium Oxalate / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D028521:Musa / Q000737:chemistry*; D010070:Oxalates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D018547:Plant Stems / Q000737:chemistry*; D014545:Urinary Calculi / Q000737:chemistry / Q000378:metabolism / Q000517:prevention & control; D025101:Vitamin B 6 / Q000494:pharmacology*
D010070:Oxalates; D010936:Plant Extracts; D002129:Calcium Oxalate; D025101:Vitamin B 6; D002118:Calcium