Production of teicoplanin by valine analogue-resistant mutant strains of Actinoplanes teichomyceticus.
Teicoplanin is a glycopeptide antibiotic produced by Actinoplanes teichomyceticus. A strain improvement to increase the productivity of the major component, teicoplanin A2-2, was carried out. As the fatty moiety of teicoplanin A2-2 is derived from L-valine, L-valine analogue (valine hydroxamate)-resistant mutants were derived. One of the mutants, 98-1-227, overproduced valine and produced a higher titer of total teicoplanin with higher A2-2 content. In a pilot fermentor (7 m3), the total productivity of teicoplanin was 1,800 units/ml and the A2-2 content was 58%.
Jin
Z H
ZH
Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou, PR China. zjujzh@yahoo.com
Wang
M R
MR
Cen
P L
PL
D000192:Actinomycetales / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005285:Fermentation; D009154:Mutation; D017334:Teicoplanin / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000494:pharmacology; D014633:Valine / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D017334:Teicoplanin; D014633:Valine