Rapid accumulation and internalization of radiolabeled herceptin in an inflammatory breast cancer xenograft with vasculogenic mimicry predicted by the contrast-enhanced dynamic MRI with the macromolecular contrast agent G6-(1B4M-Gd)(256).
The rapid blood flow and perfusion of macromolecules in the inflammatory breast cancer xenograft (WIBC-9), which exhibits a "vasculogenic mimicry" type of angiogenesis without the participation of endothelial cells and expresses high levels of the HER-2/neu antigen, was evaluated in mice using 3D-micro-MR angiography using a novel macromolecular MR contrast agent [G6-(1B4M-Gd)(256)]. Herceptin, which recognizes the HER-2/neu antigen and has similar size (10 nm) to G6-(1B4M-Gd)(256), accumulated and internalized in the WIBC-9 tumors more quickly than in the control MC-5 tumors that progress with normal angiogenesis. Three dimensional micro-MRI with the G6-(1B4M-Gd)(256) macromolecular MRI contrast agent distinguishes between the different types of angiogenesis and is predictive of the rapid accumulation and internalization of Herceptin in the WIBC-9 inflammatory breast cancer xenograft.
Kobayashi
Hisataka
H
Chaired Department of Diagnostic and Interventional Imagiology, Hitachi Medical Co., Kyoto 606-8507, Japan.
Shirakawa
Kazuo
K
Kawamoto
Satomi
S
Saga
Tsuneo
T
Sato
Noriko
N
Hiraga
Akira
A
Watanabe
Ichiro
I
Heike
Yuji
Y
Togashi
Kaori
K
Konishi
Junji
J
Brechbiel
Martin W
MW
Wakasugi
Hiro
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D061067:Antibodies, Monoclonal, Humanized; D001943:Breast Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism*; D003287:Contrast Media; D006801:Humans; D007205:Indium Radioisotopes; D018810:Magnetic Resonance Angiography / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging / Q000378:metabolism; D009942:Organometallic Compounds; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D000068878:Trastuzumab
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D003287:Contrast Media; C448324:G6-((4-isothiocyanatobenzyl)-gadolinium)256; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D000068878:Trastuzumab