title: Preparative electrophoresis: a general method for the purification of polyclonal antibodies.
abstract: Antibodies were purified from normal rabbit, sheep, goat, rat, human and bovine serum using preparative electrophoresis on a Gradiflow in a single-step process using an asymmetrical cartridge with three different pore size polyacrylamide membranes. Recoveries in each case were over 80% and were higher than those obtained using affinity chromatography on protein A, protein G or protein L. Degree of purity was at least comparable with these methods. These results suggest that preparative electrophoresis can be considered a general method for the purification of research quantities of antibodies from multiple serum sources and may be particularly useful where the reactivity with protein A, G or L is unknown.
authors.affiliation: Gradipore Ltd., Frenchs Forest, NSW, Australia. tthomas@gradipore.com
authors.forename: T M
authors.initials: TM
authors.lastname: Thomas
authors.forename: E E
authors.initials: EE
authors.lastname: Shave
authors.forename: I M
authors.initials: IM
authors.lastname: Bate
authors.forename: S C
authors.initials: SC
authors.lastname: Gee
authors.forename: S
authors.initials: S
authors.lastname: Franklin
authors.forename: D B
authors.initials: DB
authors.lastname: Rylatt
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans
chemical_list: D000906:Antibodies