[CDKN2/p16 gene cloning and its probe preparation and application in lung cancer].
OBJECTIVE
To clone CDKN2/p16(INK4a) gene, prepare its probe, and to study the change of CDKN2/p16(INK4a) gene in lung cancers.


METHODS
Total RNA of normal lung tissue was extracted, CDKN2/p16(INK4a) gene cDNA synthesized, and CDKN2/p16(INK4a) gene recombinant vector, constructed. Southern blot was used to study CDKN2/p16(INK4a) gene in 46 cases of lung cancers, 3 cases of normal lung tissues, 6 cases of lung tissues near cancer, and 3 cases of lymph nodes with lung cancer metastasis.


RESULTS
Cloned CDKN2/p16(INK4a) cDNA was proved by enzyme digestion and sequencing. Southern blot showed 4.3 kb band in normal lung tissues and lung tissues near cancers, and deletion of CDKN2/p16(INK4a) gene in cancer tissues and lymph nodes with lung cancer metastasis, with a deletion rate of 17.4% (8/46).


CONCLUSIONS
CDKN2/p16(INK4a) gene may play a role to some extent in progression of lung cancers.
Su
C
C
Cancer Center of People's Liberation Army, Nanjing 81st Hospital, Nanjing 210002, China.
Ye
Y
Y
Wang
D
D
Cao
X
X
Shan
X
X
D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D017353:Gene Deletion; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D025803:Tumor Suppressor Protein p14ARF / Q000235:genetics*
D015342:DNA Probes; D025803:Tumor Suppressor Protein p14ARF