Enhancement of bile tolerance in lactococci by Tween 80.
OBJECTIVE
The aim of this study is to clarify the effect of Tween 80 on bile tolerance of lactococci.


RESULTS
Four out of the six strains of lactococci could grow in broth containing 0.3% bile in the presence of 1% Tween 80, but grew slightly or not at all in the absence of Tween 80. Growing with Tween 80 altered the fatty acid composition of all three strains tested, but it is not clear which fatty acid influences bile tolerance. Material that absorbed light at 260 nm leaked from the cells tested with bile, but the leakage was decreased by addition of 1% Tween 80. Coincidentally, the decrease in the cell count by exposure to bile was suppressed by addition of Tween 80.


CONCLUSIONS
Tween 80 enhances bile tolerance of some strains of lactococci.


CONCLUSIONS
It is clarified that Tween 80 in the broth contributes to the bile tolerance of lactococci by reducing the cellular leakage caused by bile.
Kimoto
H
H
Department of Animal Products, National Institute of Livestock and Grassland Science, Tsukuba Norin-danchi, Ibaraki, Japan. anne@affrc.go.jp
Ohmomo
S
S
Okamoto
T
T
D001646:Bile; D002463:Cell Membrane Permeability / Q000187:drug effects; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D017045:Lactococcus / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D011136:Polysorbates / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003470:Culture Media; D005227:Fatty Acids; D011136:Polysorbates; D013501:Surface-Active Agents