[Muscarinic receptor subtype-3 expression in trabecular meshwork of normal human eye and primary open-angle glaucoma].
OBJECTIVE
To identify the muscarinic receptor subtype-3 (M3-receptor) expression in trabecular meshworks (TM) of normal human eyes and eyes with primary open-angle glaucoma (POAG) and discuss the pathological changes of TM in POAG.


METHODS
TM specimens were taken from 5 normal persons and 10 patients with POAG at late stage. Then LSAB immunohistochemical technique was used to identify M3-receptors. The results were observed under microscope and compared with computer-image-analysis system.


RESULTS
(1) The trabecular meshwork cells in all the 5 normal persons expressed M3-receptors. The positive cells mainly existed in the uveal part of TM, from Schwalbe's line to scleral spur. (2) In TM from eyes with POAG, the amount of both TM cells and M3-receptor positive cells were reduced obviously, even some specimens had no such positive expression. The positive cells were correspondingly decreased in number and scattered in distribution.


CONCLUSIONS
The TM cells in normal human eyes express M3-receptors. The TM cells and M3-positive cells in eyes with late POAG are reduced in number and their distribution is irregular.
Luo
S
S
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Peng
D
D
Zeng
S
S
D000293:Adolescent; D000328:Adult; D005260:Female; D005902:Glaucoma, Open-Angle / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic / Q000096:biosynthesis*; D014129:Trabecular Meshwork / Q000378:metabolism* / Q000473:pathology
D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic