Expression of the aromatase cytochrome P450 encoding gene in cattle and sheep.
During this report the tissue-specific expression and promoter usage of the aromatase cytochrome P450 encoding gene, Cyp19, are compared between cattle and sheep. In addition, data will be presented on the identification of cis-acting regulatory sequences located in the bovine placenta-specific promoter 1.1. In cattle and sheep Cyp19 is mainly expressed in the foetal placental layer and ovarian granulosa cells but also in other organs as brain or testis. Differently spliced transcripts of Cyp19 which include an invariable coding region but a variable 5'-untranslated region could be detected in tissues of both species. However, in contrast to ovary and brain which express homologous transcript variants, different transcripts are present in placentae suggesting that also different placenta-specific promoter regions are active in cattle and sheep. The analysis of the chromatin structure of the main placental promoter 1.1 in different bovine tissues revealed that hypomethylation and the occurrence of DNaseI hypersensitive sites (HS) within this region are associated with promoter activity. Active regulatory elements were identified in reporter gene studies in JEG-3 choriocarcinoma cells. The co-localisation of an E-box element within one of the placenta-specific HS suggests that this element is important for Cyp19 expression in the bovine placenta.
Vanselow
J
J
Research Institute for the Biology of Farm Animals, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany. vanselow@fbn-dummerstorf.de
Fürbass
R
R
Zsolnai
A
A
Kalbe
C
C
Said
H M
HM
Schwerin
M
M
D017398:Alternative Splicing; D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002843:Chromatin / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013045:Species Specificity
D002843:Chromatin; D004247:DNA; D001141:Aromatase