title: Large offspring syndrome and other consequences of ruminant embryo culture in vitro: relevance to blastocyst culture in human ART.
abstract: In vitro production of embryos from domestic animals is used to augment conventional genetic improvement programmes in agriculture and to facilitate advances in gene transfer and cloning. However, production of embryos in vitro exposes them to hazards not normally encountered in vivo and, as a result, there have been unforeseen consequences including the large offspring syndrome. This syndrome is manifest as abnormal growth and development at fetal, neonatal and later stages after transfer of embryos cultured in vitro for up to 1 week after fertilization. Our embryo culture and fetal development studies have begun to characterize many of the genetic, metabolic and developmental features associated with the syndrome. This review considers the findings of these studies in the context of blastocyst production in vitro, emphasizing the impact of culture strategies on ruminant (cattle and sheep) embryo composition and developmental competence. The need to alter in vitro production strategies to safeguard oocytes and embryos during culture is discussed. Finally, the implications of experiences gained in domestic animal studies are considered in the context of current options for human embryo culture. The need for an appreciation of the sensitivity of the embryo to its environment and the possible short- and long-term consequences of inappropriate in vitro production strategies are considered.
authors.affiliation: Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK.
authors.forename: Thomas G.
authors.initials: TG
authors.lastname: McEvoy
authors.forename: Kevin D.
authors.initials: KD
authors.lastname: Sinclair
authors.forename: Lorraine E.
authors.initials: LE
authors.lastname: Young
authors.forename: Ian
authors.initials: I
authors.lastname: Wilmut
authors.forename: John J.
authors.initials: JJ
authors.lastname: Robinson