title: Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.
abstract: The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.
authors.affiliation: Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. hirosek@nih.go.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Hirose
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Itoh
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakajima
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kurazono
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Moriya
authors.forename: Takayuki
authors.initials: T
authors.lastname: Ezaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Tamura
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D006801:Humans; D010284:Paratyphoid Fever / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012485:Salmonella typhi / Q000145:classification* / Q000235:genetics / Q000276:immunology; D000637:Transaminases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014435:Typhoid Fever / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D014157:Transcription Factors; C098634:tviA protein, Salmonella typhi; D005408:Flagellin; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061271:paratose synthase; C061272:perosamine synthetase