title: [Histological study of conjunctiva-associated lymphoid tissue in mice].
abstract: OBJECTIVE
  To investigate conjunctiva-associated lymphoid tissue (CALT) in mouse conjunctiva.
  
  
  METHODS
  We examined normal mice, ranging in age from 4 to 6 weeks, to investigate the presence of follicular tissue histologically. Next, we treated mice by topical instillation of ovalbumin and cholera toxin B, and then examined conjunctival follicles by immunohistological methods.
  
  
  RESULTS
  Follicular tissue was present at the nictitating membrane. Both size and amount of follicular tissue was increased by the topical ovalbumin treatment. Immunohistological examination revealed CD4, CD8, and S-100 protein positive cells in follicular tissue. The epithelial layer, corresponding to follicular tissue, had intraepithelial pockets and CD4 positive cells in the pockets.
  
  
  CONCLUSIONS
  It was concluded that the follicular tissue at the nictitating membrane is CALT in mice.
authors.affiliation: Department of Ophthalmology, Nihon University School of Medicine, 30-1 Ooyaguchikami-machi, Itabashi-ku, Tokyo 173-8610, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Sakimoto
authors.forename: Jun
authors.initials: J
authors.lastname: Shoji
authors.forename: Keiko
authors.initials: K
authors.lastname: Saito
authors.forename: Noriko
authors.initials: N
authors.lastname: Inada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Sawa
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D003228:Conjunctiva / Q000166:cytology* / Q000276:immunology; D005260:Female; D008221:Lymphoid Tissue / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009418:S100 Proteins / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D009418:S100 Proteins