title: Differences in editing of mitochondrial nad3 transcripts from CMS and fertile carrots.
abstract: A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
authors.affiliation: Department of Plant Molecular Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznań, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Rurek
authors.forename: M
authors.initials: M
authors.lastname: Szklarczyk
authors.forename: N
authors.initials: N
authors.lastname: Adamczyk
authors.forename: B
authors.initials: B
authors.lastname: Michalik
authors.forename: H
authors.initials: H
authors.lastname: Augustyniak
mesh_terms: D000595:Amino Acid Sequence; D003062:Codon / Q000235:genetics; D018552:Daucus carota / Q000166:cytology / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins; D010940:Plant Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D017393:RNA Editing / Q000235:genetics*; D000077278:RNA, Mitochondrial; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003062:Codon; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D000077278:RNA, Mitochondrial; D018749:RNA, Plant; C103919:Rps12 protein, plant; D012313:RNA