Expression of Notch ligands, Jagged1, 2 and Delta1 in antigen presenting cells in mice.
Notch1 is indispensable for T cell development. It is anticipated that Notch1 and other Notch receptors expressed on the surface of thymic T cell precursors are activated by ligands present on environmental cells, including antigen presenting cells (APCs), and involved in positive and negative selections. Notch receptors on peripheral T cells may also be activated by ligands on APCs. Here, we examined the expression pattern of three Notch ligands, Jagged1, 2 and Delta1 in APCs by an immunofluorescence cell staining method and a reverse transcriptase-polymerase chain reaction (RT-PCR) method. Peritoneal macrophages were strongly positive for Jagged1 staining. In contrast, macrophages separated from spleen and dendritic cells (DCs) separated from spleen and thymus showed positive staining for all the three ligands at a similar intensity. An analysis by RT-PCR revealed that peritoneal and splenic macrophages and splenic and thymic DCs, show a distinct pattern in Notch ligand expression. These findings may represent that expression of various Notch ligands in APCs has a physiological relevance in each organ.
Yamaguchi
Etsuko
E
Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Chiba
Shigeru
S
Kumano
Keiki
K
Kunisato
Atsushi
A
Takahashi
Tokiharu
T
Takahashi
Tsuyoshi
T
Hirai
Hisamaru
H
D000818:Animals; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000276:immunology; D002469:Cell Separation; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007136:Immunoglobulins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008024:Ligands; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology; D018121:Receptors, Cytokine / Q000096:biosynthesis* / Q000276:immunology; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D007136:Immunoglobulins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; C490503:Jag2 protein, mouse; D000072100:Jagged-1 Protein; D000072101:Jagged-2 Protein; D008024:Ligands; D008565:Membrane Proteins; D011506:Proteins; D018121:Receptors, Cytokine; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; C410591:Tslpr protein, mouse