[Homozygous deletion of p16 and p15 genes in laryngeal squamous cell carcinoma].
OBJECTIVE
To assess the relationship of homozygous deletion status of p16 (MTS1/INK4a/CDKN2A), p15(MTS2/INK4b/CDKN2B) genes and laryngeal squamous cell carcinoma(LSCC) progression.


METHODS
DNA was extracted from fresh tumors. Homozygous deletion of p16 exon 2(p16E2) in 80 cases of LSCC and p15 exon 2(p15E2) in 67 cases of LSCC were detected by the polymerase chain reaction technique.


RESULTS
The p16E2 deletion rate in 80 cases was 12.5%(10/80); the p15E2 deletion rate in 67 cases was 11.94%(8/67); the p16E2 and p15E2 codeletion rate in 67 cases was 5.97%(4/67).


CONCLUSIONS
Homozygous deletion of p16E2 and p15E2 is related with LSCC oncogenesis, and it may play a role to some extent in LSCC malignant progression.
Li
Fucai
F
Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 P. R. China. Fucaili@hotmail.com
Kang
Ning
N
Li
Yinghui
Y
He
Guang
G
Lin
Changkun
C
Sun
Xinghe
X
Gao
Hongming
H
Sun
Kailai
K
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D017353:Gene Deletion; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D007438:Introns / Q000235:genetics; D007822:Laryngeal Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005819:Genetic Markers; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins