[The effect of 1, 25 (OH)(2)D(3)on growth and apoptosis in breast cancer cell line MCF-7].
OBJECTIVE
To study the effect of 1, 25-dihydroxyvitamin D(3) [1, 25 (OH)(2)D(3)] on growth and apoptosis in breast cancer cell line MCF-7.


METHODS
We compared cell numbers by using MTT method, analyzed cell cycle and apoptosis percentage by flow cytometric, observed cellular structure and ultrastructure, determined quantitatively apoptosis cells by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and detected expression of bcl-2 protein by Western Blot.


RESULTS
Incubation with 1, 25 (OH)(2)D(3) 10(-7) mol/L, MCF-7 cells exhibited significant growth inhibition. Flow cytometric analysis indicated cell's G(0)/G(1) arrest along with increasing apoptotic peak and percentage. The action of cytotoxic drug Adr was strengthened. Western Blot showed that 1, 25 (OH)(2)D(3) could down-regulate bcl-2 protein expression.


CONCLUSIONS
1, 25 (OH)(2)D(3) could be a new hormone for the treatment of breast cancer.
Zhang
J
J
Department of General Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016.
Yao
Z
Z
D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
D018931:Antineoplastic Agents, Hormonal; D019253:Proto-Oncogene Proteins c-bcl-2; D002117:Calcitriol