title: Deletion pattern of the STS gene in X-linked ichthyosis in a Mexican population.
abstract: BACKGROUND
  X-linked ichthyosis (XLI) is an inherited disorder due to steroid sulfatase deficiency (STS). Most XLI patients (>90%) have complete deletion of the STS gene and flanking sequences. The presence of low copy number repeats (G1.3 and CRI-S232) on either side of the STS gene seems to play a role in the high frequency of these interstitial deletions. In the present study, we analyzed 80 Mexican patients with XLI and complete deletion of the STS gene.
  
  
  METHODS
  STS activity was measured in the leukocytes using 7-[(3)H]-dehydroepiandrosterone sulfate as a substrate. Amplification of the regions telomeric-DXS89, DXS996, DXS1139, DXS1130, 5' STS, 3' STS, DXS1131, DXS1133, DXS237, DXS1132, DXF22S1, DXS278, DXS1134-centromeric was performed through PCR.
  
  
  RESULTS
  No STS activity was detected in the XLI patients (0.00 pmoles/mg protein/h). We observed 3 different patterns of deletion. The first two groups included 25 and 32 patients, respectively, in which homologous sequences were involved. These subjects showed the 5' STS deletion at the sequence DXS1139, corresponding to the probe CRI-S232A2. The group of 32 patients presented the 3' STS rupture site at the sequence DXF22S1 (probe G1.3) and the remaining 25 patients had the 3' STS breakpoint at the sequence DXS278 (probe CRI-S232B2). The third group included 23 patients with the breakpoints at several regions on either side of the STS gene. No implication of the homologous sequences were observed in this group.
  
  
  CONCLUSIONS
  These data indicate that more complex mechanisms, apart from homologous recombination, are occurring in the genesis of the breakpoints of the STS gene of XLI Mexican patients.
authors.affiliation: Servicio de Genetica, Hospital General de Mexico, Facultad de Medicina, UNAM, Mexico D.F., MX.
authors.forename: A L
authors.initials: AL
authors.lastname: Jimenez Vaca
authors.forename: M del R
authors.initials: Mdel R
authors.lastname: Valdes-Flores
authors.forename: M R
authors.initials: MR
authors.lastname: Rivera-Vega
authors.forename: L M
authors.initials: LM
authors.lastname: González-Huerta
authors.forename: S H
authors.initials: SH
authors.lastname: Kofman-Alfaro
authors.forename: S A
authors.initials: SA
authors.lastname: Cuevas-Covarrubias
mesh_terms: D001192:Arylsulfatases / Q000172:deficiency / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D016114:Ichthyosis, X-Linked / Q000201:enzymology / Q000235:genetics*; D008800:Mexico; D043266:Steryl-Sulfatase
chemical_list: D001192:Arylsulfatases; D043266:Steryl-Sulfatase