Preparative electrophoresis: a general method for the purification of polyclonal antibodies.
Antibodies were purified from normal rabbit, sheep, goat, rat, human and bovine serum using preparative electrophoresis on a Gradiflow in a single-step process using an asymmetrical cartridge with three different pore size polyacrylamide membranes. Recoveries in each case were over 80% and were higher than those obtained using affinity chromatography on protein A, protein G or protein L. Degree of purity was at least comparable with these methods. These results suggest that preparative electrophoresis can be considered a general method for the purification of research quantities of antibodies from multiple serum sources and may be particularly useful where the reactivity with protein A, G or L is unknown.
Thomas
T M
TM
Gradipore Ltd., Frenchs Forest, NSW, Australia. tthomas@gradipore.com
Shave
E E
EE
Bate
I M
IM
Gee
S C
SC
Franklin
S
S
Rylatt
D B
DB
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans
D000906:Antibodies