Taxonomic study of extreme halophilic archaea isolated from the "Salar de Atacama", Chile.
A large number of halophilic bacteria were isolated in 1984-1992 from the Atacama Saltern (North of Chile). For this study 82 strains of extreme halophilic archaea were selected. The characterization was performed by using the phenotypic characters including morphological, physiological, biochemical, nutritional and antimicrobial susceptibility test. The results, together with those from reference strains, were subjected to numerical analysis, using the Simple Matching (S(SM)) coefficient and clustered by the unweighted pair group method of association (UPGMA). Fifteen phena were obtained at an 70% similarity level. The results obtained reveal a high diversity among the halophilic archaea isolated. Representative strains from the phena were chosen to determine their DNA base composition and the percentage of DNA-DNA similarity compared to reference strains. The 16S rRNA studies showed that some of these strains constitutes a new taxa of extreme halophilic archaea.
Lizama
C
C
Department of Microbiology, Faculty of Pharmacy, University of Granada, Spain.
Monteoliva-Sánchez
M
M
Prado
B
B
Ramos-Cormenzana
A
A
Weckesser
J
J
Campos
V
V
D001105:Archaea / Q000145:classification* / Q000302:isolation & purification; D002677:Chile; D019641:DNA, Archaeal / Q000032:analysis; D010641:Phenotype; D010802:Phylogeny
D019641:DNA, Archaeal