Muscle type-specific response of HSP60, HSP72, and HSC73 during recovery after elevation of muscle temperature.
An original method to induce heat stress was used to clarify the time course of changes in heat shock proteins (HSPs) in rat skeletal muscles during recovery after a single bout of heat stress. One hindlimb was inserted into a stainless steel can and directly heated by raising the air temperature inside the can via a flexible heater twisted around the steel can. Muscle temperature was increased gradually and maintained at 42 degrees C for 60 min. Core rectal and contralateral muscle temperatures were increased <1.5 degrees C during the heat stress. HSP60, HSP72, and heat shock cognate (HSC) 73 content in the slow soleus and fast plantaris in both limbs were determined immediately (0 h) and 2, 4, 8, 12, 24, 36, 48, or 60 h after heat stress. Within 0-4 h, all HSPs were approximately 1.5- to 2.2-fold higher in heat-stressed than contralateral soleus. Compared with the contralateral plantaris, the heat-stressed plantaris had a higher (1.5-fold) HSP60 content immediately and 2 h after heat stress and a higher (2.5- to 6.8-fold) HSP72 content between 24 and 48 h after heat stress. Plantaris HSC73 content was not affected by heat stress. This unique heat-stress method provides advantages over existing systems; muscle temperature can be controlled precisely during heating and the HSP response can be compared between muscles in heat-stressed and contralateral limbs of individual rats. Results show a differential response of HSPs in the soleus and plantaris during recovery after heat stress; soleus demonstrated a more rapid and broader HSP response to heat stress than plantaris.
Oishi
Yasuharu
Y
Laboratory of Muscle Physiology, Faculty of Education, Kumamoto University, Kumamoto 860-8555, Japan. oishi@gpo.kumamoto-u.ac.jp
Taniguchi
Kouhachi
K
Matsumoto
Hisahiro
H
Ishihara
Akihiko
A
Ohira
Yoshinobu
Y
Roy
Roland R
RR
D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D018834:Chaperonin 60 / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D018882:Heat Stress Disorders / Q000503:physiopathology; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006614:Hindlimb; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012007:Rectum / Q000502:physiology
D018834:Chaperonin 60; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C494373:Hspa8 protein, rat