title: [Genescan analysis of non-small cell lung cancer in the long arm of chromosome 6].
abstract: OBJECTIVE
  To investigate if there are microsatellite loci in the long arm of chromosome 6 that have close relationship with non-small cell lung cancer.
  
  
  METHODS
  Multiple PCR approach was used to analyze the 18 loci in the long arm of chromosome 6. The PCR products were analyzed in PAGE, and then the electrophoresis maps were analyzed with Gene Scan(TM) and Genotyper(TM).
  
  
  RESULTS
  There were different frequencies of loss of heterozygosity (LOH) in different loci (varying from 3.85% to 38.45%). The total frequency of LOH in 41 gastric cancers was 58.5%(24/41). Eight loci with the LOH frequency higher than 20% were mainly located in 2 regions: 6q24 and 6q27. The accurate location is 6q24-6q25.3 [D6S1699(35%), D6S409(23.33%), D6S441(33.33%)] and 6q26-27 [D6S1550(38.45%), D6S264(20%), D6S1585(25%), D6S446(33.33%), D6S281(30.77%)].
  
  
  CONCLUSIONS
  There may be tumor suppressor genes located in the region of 6q24 and 6q27, which have close relationship with non-small cell lung cancer.
authors.affiliation: Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150086 P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Baiqiu
authors.initials: B
authors.lastname: Wang
authors.forename: Chenghui
authors.initials: C
authors.lastname: Yan
authors.forename: Shengfa
authors.initials: S
authors.lastname: Wang
authors.forename: Baichun
authors.initials: B
authors.lastname: Wang
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.forename: Chengbin
authors.initials: C
authors.lastname: Huang
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods