Mechanism of response enhancement in evaporative light scattering detection with the addition of triethylamine and formic acid.
Triethylamine with an equimolar amount of formic acid added to the mobile phase provides an enhancement of the evaporative light scattering detector (ELSD) response. After characterization of the influence of various parameters on the ELSD response, a sequential strategy was defined to elucidate this response enhancement. The response enhancement was more marked at low mobile phase flow rate, and was highly dependent on solutes and solvents. The influence of drift tube temperature on response enhancement with various solutes demonstrated that triethylamine and formic acid mainly act as mass amplifiers by the inclusion of triethylamine-formic acid clusters inside the droplets.
Deschamps
Frantz S
FS
Laboratoire de Chimie Analytique, Faculté de Pharmacie, Châtenay-Malabry, France. Frantz.Deschamps@cep.u-psud.fr
Baillet
Arlette
A
Chaminade
Pierre
P
D002853:Chromatography, Liquid / Q000379:methods; D005021:Ethylamines; D017022:Flow Injection Analysis; D005561:Formates; D008027:Light; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012542:Scattering, Radiation
D005021:Ethylamines; D005561:Formates; D004364:Pharmaceutical Preparations; C030544:formic acid; C016162:triethylamine