title: Cytoskeletal regulation by the Nedd8 ubiquitin-like protein modification pathway.
abstract: The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements during Caenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
authors.forename: Thimo
authors.initials: T
authors.lastname: Kurz
authors.forename: Lionel
authors.initials: L
authors.lastname: Pintard
authors.forename: John H
authors.initials: JH
authors.lastname: Willis
authors.forename: Danielle R
authors.initials: DR
authors.lastname: Hamill
authors.forename: Pierre
authors.initials: P
authors.lastname: Gönczy
authors.forename: Matthias
authors.initials: M
authors.lastname: Peter
authors.forename: Bruce
authors.initials: B
authors.lastname: Bowerman
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D044842:Cullin Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism; D017238:Genes, Helminth; D000074201:Katanin; D008540:Meiosis; D008870:Microtubules / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008938:Mitosis; D009154:Mutation; D015739:Nocodazole / Q000494:pharmacology; D018510:RNA, Helminth / Q000235:genetics; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure; D014452:Ubiquitins / Q000235:genetics / Q000378:metabolism*
chemical_list: C105153:CUL2 protein, human; C503645:CUL3 protein, human; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D044842:Cullin Proteins; D018510:RNA, Helminth; D014452:Ubiquitins; D000251:Adenosine Triphosphatases; C086678:MEI-1 protein, C elegans; D000074201:Katanin; D015739:Nocodazole