title: Nucleic acid supercoiling as a means for ionic switching of DNA--nanoparticle networks.
abstract: Oligomeric nanoparticle networks, generated by the self-assembly of bis-biotinylated double-stranded DNA fragments and streptavidin, have been studied by scanning force microscopy (SFM). SFM imaging revealed the presence within the networks of irregular thick DNA molecules, which were often associated with distinct, Y-shaped structural elements. Closer analysis revealed that the Y structures are formed by condensation (thickening and shortening) of two DNA fragments, most likely through the supercoiling of two DNA molecules bound to adjacent binding sites of the streptavidin particle. The frequency of supercoiling was found to be dependent on the ionic strength applied during the immobilization of the oligomeric networks on mica surfaces. Potential applications of the structural changes as a means for constructing ion-dependent molecular switches in nanomaterials are discussed.
authors.affiliation: Universität Bremen, FB2-UFT Biotechnologie und Molekulare Genetik, Leobener Strasse, 28359 Bremen, Germany. cmn@biotec.uni-bremen.de
authors.forename: C M
authors.initials: CM
authors.lastname: Niemeyer
authors.forename: M
authors.initials: M
authors.lastname: Adler
authors.forename: S
authors.initials: S
authors.lastname: Lenhert
authors.forename: S
authors.initials: S
authors.lastname: Gao
authors.forename: H
authors.initials: H
authors.lastname: Fuchs
authors.forename: L
authors.initials: L
authors.lastname: Chi
mesh_terms: D019921:Biotinylation; D004278:DNA, Superhelical / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology / Q000379:methods*; D019809:Streptavidin
chemical_list: D004278:DNA, Superhelical; D019809:Streptavidin