title: Immunohistochemical detection of hepatocellular carcinoma in the setting of ongoing necrosis after radiofrequency ablation.
abstract: After radiofrequency ablation (RFA), hepatocellular carcinoma undergoes complete necrosis and an ongoing necrosis that is irreversible and characterized histologically by disrupted cell outlines, homogenous cytoplasmic eosinophilia, and preserved nuclear staining, with the cells appearing quite distinct from viable cancer cells. Antibody to detect single-stranded DNA (ssDNA) specifically labeled nuclei in the setting of ongoing necrosis, but not viable tumor cells, whereas human mitochondrial antibody labeled the cytoplasm of viable cells but not cells of ongoing necrosis. The results demonstrate that RFA causes denaturation of both DNA and proteins and that the immunohistochemistry of ssDNA and mitochondrial protein is useful in detection of ongoing necrosis after RFA and provides pathological information on the validity of this procedure.
authors.affiliation: Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, Hokkaido, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Itoh
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Orba
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Takei
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ishida
authors.forename: Makoto
authors.initials: M
authors.lastname: Saitoh
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Meguro
authors.forename: Shoichi
authors.initials: S
authors.lastname: Horita
authors.forename: Miri
authors.initials: M
authors.lastname: Fujita
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagashima
mesh_terms: D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D017115:Catheter Ablation; D002467:Cell Nucleus / Q000737:chemistry / Q000473:pathology; D004277:DNA, Single-Stranded / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000737:chemistry / Q000473:pathology; D009336:Necrosis
chemical_list: D004277:DNA, Single-Stranded