title: A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts.
abstract: A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCl to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38 +/- 4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.
authors.affiliation: Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Guerrieri
authors.forename: L
authors.initials: L
authors.lastname: Monaci
authors.forename: M
authors.initials: M
authors.lastname: Quinto
authors.forename: F
authors.initials: F
authors.lastname: Palmisano
mesh_terms: D015374:Biosensing Techniques; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004209:Disposable Equipment; D004563:Electrochemistry; D007306:Insecticides / Q000032:analysis*; D009943:Organophosphorus Compounds; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D012989:Soil Pollutants