title: [Comparison of three ELISA techniques for the evaluation of IgG seroprevalence against Bordetella pertussis in children vaccinated with three doses of DTPwc].
abstract: BACKGROUND
  Although there are several ELISA procedures to determine IgG against Bordetella pertussis, there are scarce data that allow to compare the seroprevalence detected using different kits. The objective of this study was to evaluate and to compare by three ELISA techniques the seroprevalence against B. pertussis in children.
  
  
  METHODS
  172 children 6-10 years old, vaccinated with three dose of DTPwc were studied. IgG against Bordetella pertussis was determined by two quantitative techniques (Labsystems and Serion). The qualitative detection of IgG against FHA and PT was performed by a semiquantitative method (Pertusscan).
  
  
  RESULTS
  The Labsystems ELISA classified a 42.4% of the samples as positive and a 32% as borderline. The Serion method showed 52.9% of positive and 20.9% of borderline results. IgG-FHA was detected in 81.4% and IgG-PT in 75% of the samples by Pertusscan. The level of "immunity" recommended by this method was of 21.5%. The agreement between Labsystems and Serion was 45.9% (kappa index 5 0.157; p < 0.01). In the IgG-TP or IgG-FHA positive samples, the proportion of positive results obtained by Serion was superior to those obtained by Labsystems while the number of borderline results were inferior (p < 0.05).
  
  
  CONCLUSIONS
  Both quantitative techniques showed a high rate of not conclusive results and a very weak concordance. The large number of doubtful results obtained by Labsystems and its worse concordance with the other techniques dissuades its employment in inmunogenicity studies. The results obtained by the other two techniques present a problematic interpretation in terms of seroprevalence.
authors.affiliation: Laboratorio Regional de Salud Pública, Dirección General de Salud Pública. Consejería de Sanidad de la Comunidad de Madrid. jcsanz1@teleline.es
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Sanz
authors.forename: Marisa
authors.initials: M
authors.lastname: Fernández
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Sagües
authors.forename: Rosa
authors.initials: R
authors.lastname: Ramírez
authors.forename: Rosario
authors.initials: R
authors.lastname: Castañeda
authors.forename: Dolores
authors.initials: D
authors.lastname: Barranco
authors.forename: Fernando
authors.initials: F
authors.lastname: de Ory
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology*; D002648:Child; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007115:Immunization Schedule; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D016036:Seroepidemiologic Studies; D014611:Vaccination; D010566:Virulence Factors, Bordetella / Q000276:immunology
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D006388:Hemagglutinins; D007074:Immunoglobulin G; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis