Synergistic cytotoxic action of vitamin C and vitamin K3.
We investigated the combination effect of sodium ascorbate (vitamin C) and menadione (vitamin K3) on the viability of various cultured cells. Human oral squamous cell carcinoma (HSC-2, HSC-3) and human promyelocytic leukemia (HL-60) cells were more sensitive to these vitamins as compared to normal cells (human gingival fibroblast HGF, human periodontal ligament fibroblast HPLF, human pulp cell HPC). The combination of vitamin C and vitamin K3 produced synergistic cytotoxicity against all these 6 cell lines. Treatment with vitamin C or vitamin K3, or their combination, induced internucleosomal DNA fragmentation only in HL-60 cells, but not in the oral tumor cell lines (HSC-2, HSC-3, HSG). ESR spectroscopy showed that vitamins C and K3 produce radicals under alkaline conditions and that the combination of these two vitamins synergistically enhanced their respective radical intensities.
Zhang
W
W
Department of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing, China.
Negoro
T
T
Satoh
K
K
Jiang
Y
Y
Hashimoto
K
K
Kikuchi
H
H
Nishikawa
H
H
Miyata
T
T
Yamamoto
Y
Y
Nakano
K
K
Yasumoto
E
E
Nakayachi
T
T
Mineno
K
K
Satoh
T
T
Sakagami
H
H
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000378:metabolism; D014407:Tumor Cells, Cultured; D024483:Vitamin K 3 / Q000008:administration & dosage / Q000494:pharmacology*
D024483:Vitamin K 3; D001205:Ascorbic Acid