title: [PCR walking in cotton genome using YADE method].
abstract: A Y-shaped adaptor dependent extension (YADE) method was developed to amplify the unknown genomic sequences adjacent to a known sequence, with the single-primer amplification completely suppressed. Using this method, two fragments, F027S and F027A, adjacent to a cotton ovule cDNA fragment (F027), were amplified using genomic DNA. It was demonstrated that F027S and F027A had 104 bp and 175 bp overlapped to F027, and contained 1 and 3 putative introns, respectively, all of which included conserved border sequence GT-AG and a putative branch site. The possible improvements and applications of YADE method are further discussed.
authors.affiliation: Biotechnology Research Center, Southwest Agricultural University, Chongqing 400716, China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Xiao
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Fang
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Hou
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary; D018744:DNA, Plant / Q000032:analysis; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant