Isolation of porcine expressed sequence tags for the construction of a first genomic transcript map of the skeletal muscle in pig.
To identify genes with effects on meat quality and production traits we developed an adult porcine skeletal muscle cDNA library. After pre-screening this library with seven genes highly expressed in skeletal muscle, 385 non-hybridizing clones were sequenced from both ends to yield 510 expressed sequence tags (ESTs). Together with those ESTs previously generated from this library, we have produced 701 porcine skeletal muscle ESTs. These ESTs were grouped into 306 different cDNA species and compared with the human skeletal muscle transcriptional profiles obtained from different databases. Furthermore we mapped 107 of these cDNAs using a somatic cell hybrid panel with genes mapping over all the autosomes (except on chromosome 11) and on chromosome X. The mapping of these cDNAs contributed to the construction of a first genomic transcript map of the skeletal muscle tissue in pig.
Davoli
R
R
DIPROVAL - Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy. roberta.davoli@stpa.unibo.it
Fontanesi
L
L
Zambonelli
P
P
Bigi
D
D
Gellin
J
J
Yerle
M
M
Milc
J
J
Braglia
S
S
Cenci
V
V
Cagnazzo
M
M
Russo
V
V
D000818:Animals; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D016678:Genome; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D013552:Swine / Q000235:genetics*; D026801:Synteny; D014158:Transcription, Genetic
D018076:DNA, Complementary