title: Light-induced unfolding of photoactive yellow protein mutant M100L.
abstract: Light-activation of the PAS domain protein photoactive yellow protein (PYP) is believed to trigger a negative phototactic response in the phototropic bacterium Halorhodospira halophila. To investigate transient conformational changes of the PYP photocycle, we utilized the PYP mutant M100L that displays an increased lifetime of the putative signaling-state photointermediate PYP(M) by 3 orders of magnitude, as previously reported for the M100A mutant [Devanathan, S., Genick, U. K., Canestrelli, I. L., Meyer, T. E., Cusanovich, M. A., Getzoff, E. D., and Tollin, G. Biochemistry (1998) 37, 11563-11568]. The FTIR difference spectrum of PYP(M) and the ground state of M100L demonstrated extensive peptide-backbone structural changes as observed in the FTIR difference spectrum of the wild-type protein and PYP(M). The conformational change investigated by CD spectroscopy in the far-UV region showed reduction of the alpha-helical content by approximately 40%, indicating a considerable amount of changes in the secondary structure. The optical activity of the p-coumaric acid chromophore completely vanished upon PYP(M) in contrast to the dark state, indicating deformation of the binding pocket structure in PYP(M). The tertiary structural changes were further monitored by small-angle X-ray scattering measurements, which demonstrated a significant increase of the radius of gyration of the molecule by approximately 5% in PYP(M). These structural changes were reversed concomitantly with the chromophore anionization upon the dark state recovery. The observed changes of the quantities provided a more vivid view of the structural changes of the mutant PYP in going from PYP(M) to PYP(dark), which can be regarded as a process of folding of the secondary and the tertiary structures of the "PAS" domain structure, coupled with the p-coumaric acid chromophore deprotonation and isomerization.
authors.affiliation: Department of Earth & Space Science, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan, and Life & Environment Division, Japan Synchrotron Radiation Research Institute (JASRI), Mikazuki, Sayo, Hyogo, 679-5198, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Sasaki
authors.forename: Masato
authors.initials: M
authors.lastname: Kumauchi
authors.forename: Norio
authors.initials: N
authors.lastname: Hamada
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Oka
authors.forename: Fumio
authors.initials: F
authors.lastname: Tokunaga
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000528:radiation effects; D002942:Circular Dichroism; D020607:Halorhodospira halophila / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010777:Photochemistry; D020283:Photoreceptors, Microbial; D011487:Protein Conformation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects; D012542:Scattering, Radiation; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D014965:X-Rays
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D011994:Recombinant Proteins; C050162:photoactive yellow protein, Bacteria