title: Crystal structure of a synthetic cyclodecapeptide for template-assembled synthetic protein design.
abstract: The structural prototype of a new generation of regioselectively addressable functionalized templates (RAFTs) for use in protein de novo design has been synthesized and crystallized. The structure of the aromatically substituted cyclodecapeptide was determined by X-ray diffraction; it consists of an antiparallel beta sheet spanned by heterochirally induced type IIprime prime or minute beta turns, similar to that observed in gramicidin S. The three-dimensional structure of the artificial template was also examined by an NMR spectroscopic analysis in solution and shown to be compatible with a beta-sheet plane suitable for accommodating secondary functional peptide fragments for the synthesis of template-assembled synthetic proteins (TASPs).
authors.affiliation: Institute of Organic Chemistry, University of Lausanne, BCH-Dorigny, 1015 Lausanne-Dorigny, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Peluso
authors.forename: T
authors.initials: T
authors.lastname: Rückle
authors.forename: C
authors.initials: C
authors.lastname: Lehmann
authors.forename: M
authors.initials: M
authors.lastname: Mutter
authors.forename: C
authors.initials: C
authors.lastname: Peggion
authors.forename: M
authors.initials: M
authors.lastname: Crisma
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary
chemical_list: D010456:Peptides, Cyclic