Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica serovars Typhi and Paratyphi A.
The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.
Hirose
Kenji
K
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. hirosek@nih.go.jp
Itoh
Ken-Ichiro
K
Nakajima
Hiroshi
H
Kurazono
Takayuki
T
Yamaguchi
Masanori
M
Moriya
Kazuo
K
Ezaki
Takayuki
T
Kawamura
Yoshiaki
Y
Tamura
Kazumichi
K
Watanabe
Haruo
H
D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D006801:Humans; D010284:Paratyphoid Fever / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012485:Salmonella typhi / Q000145:classification* / Q000235:genetics / Q000276:immunology; D000637:Transaminases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014435:Typhoid Fever / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D014157:Transcription Factors; C098634:tviA protein, Salmonella typhi; D005408:Flagellin; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061271:paratose synthase; C061272:perosamine synthetase