[Changes of elastase in gingival crevicular fluid after periodontal treatment of adult periodontitis patients].
OBJECTIVE
To examine the changes of elastase in the supernatant (EA-s) and in the pellet (EA-p) after centrifuge in gingival crevicular fluid (GCF) before and after periodontal treatment.


METHODS
GCF was sampled with filter paper strips by intra-pocket method to determine EA levels. Forty-three teeth of eight subjects were included. EA-s and EA-p were determined separately using the subtract Meosuc-ala-ala-val-pro-NA.


RESULTS
EA-s per site and per ml, EA-p per site and the ratio of EA-s/EA-p (S/P) decreased significantly after non-surgical periodontal treatment (P < 0.001), while EA-p concentration didn't change (P > 0.05).


CONCLUSIONS
GCF-EA-s and EA-p could act as objective parameters to evaluate the effect of periodontal therapy, especially EA-s and S/P, which may be sensitive diagnostic parameters for determining periodontal disease activity.
He
L
L
Department of Periodontology, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Cao
C
C
Sha
Y
Y
D000328:Adult; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000378:metabolism*; D010518:Periodontitis / Q000628:therapy*
D010196:Pancreatic Elastase