[Molecular and genetic polymorphism in three cattle breeds].
Determination of cattle breed and assignment of individual animals to that or another group based on distinctions of genotype is of great importance in genetics and selection. Using of PCR-analysis opened new possibilities for typing DNA. The procedure of PCR was optimized for animal DNA. Two methods of PCR, RAPD and SSRP, were applied for investigation of molecular-genetical polymorphism in three cattle breeds (Ukrainian red-motley lactescent, Golshtine and Simmental). A dendrogram reflecting genetical mutual relations between analyzed animals was drawn. The level of homo-heterozygosity and the distribution of microsatellite alleles were determined. System for investigation of molecular-genetical polymorphism in the cattle breeds and characterization of genotype of individual animals were developed and tested on a real population.
Zubets
M V
MV
Burkat
V P
VP
Sivolap
Iu M
IuM
Kuznetsov
V E
VE
Lovenchuk
I N
IN
D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D013045:Species Specificity; D014455:Ukraine
D004247:DNA