[The biological effect of verapamil on hypertrophic scar fibroblast].
OBJECTIVE
To study the mechanism of calcium channel antagonist Verapamil on the treatment of hypertrophic scar and explore the possibility of further clinical application.


METHODS
After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot.


RESULTS
Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil.


CONCLUSIONS
By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.
Wang
Z
Z
Center of Plastic Surgery, Xijing Hospital, Xi'an 710032, China.
Guo
S
S
Lu
K
K
D000293:Adolescent; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008297:Male; D014700:Verapamil / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D014700:Verapamil