title: A gene-driven approach to the identification of ENU mutants in the mouse.
abstract: The construction of parallel archives of DNA and sperm from mice mutagenized with ethylnitrosurea (ENU) represents a potentially powerful and rapid approach for identifying point mutations in any gene in the mouse genome. We provide support for this approach and report the identification of mutations in the gene (Gjb2) encoding connexin 26, using archives established from the UK ENU mutagenesis program.
authors.affiliation: MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell OX11 ORD, UK.
authors.forename: Emma L
authors.initials: EL
authors.lastname: Coghill
authors.forename: Alison
authors.initials: A
authors.lastname: Hugill
authors.forename: Nick
authors.initials: N
authors.lastname: Parkinson
authors.forename: Claire
authors.initials: C
authors.lastname: Davison
authors.forename: Peter
authors.initials: P
authors.lastname: Glenister
authors.forename: Sian
authors.initials: S
authors.lastname: Clements
authors.forename: Jackie
authors.initials: J
authors.lastname: Hunter
authors.forename: Roger D
authors.initials: RD
authors.lastname: Cox
authors.forename: Steve D M
authors.initials: SD
authors.lastname: Brown
mesh_terms: D000483:Alleles; D000818:Animals; D005038:Ethylnitrosourea / Q000494:pharmacology*; D005260:Female; D006579:Heterozygote; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation
chemical_list: D009153:Mutagens; D005038:Ethylnitrosourea