[Bone tissue engineering seeded with bone marrow stromal cells].
OBJECTIVE
To assess the ability of bone graft substitutes to support the growth of cells in vitro and new bone formation in vivo.


METHODS
The methods of tissue engineering were employed, the bone marrow derived stromal cells were seeded onto blocks of hydroxypatite (HA), cultured for two weeks, and implanted intermuscularly into the rabbit back muscle, scanning electron microscopy and undecalcified bone tissue sections were used to evaluate the the growth of cells in vitro and new bone formation in vivo.


RESULTS
The stromal cells were attached to and grew upon the surface of the periphery HA pores, especially in the pores adjacent to the bottom of culture flasks. No cell growth was found on the surface of the central pores. At four weeks after implantation, new bone formation was observed in most HA blocks, almost all of the new bones were lamellar bone and deposited directly on the surface of the HA pore. Active osteoblast and osteoid were found on the surface of the newly formed bone, invasion of bone marrow was observed in some newly formed bone.


CONCLUSIONS
Bone marrow stromal cells are potentially the source of seeded cells for use in bone tissue engineering, and the HA may be considered as a suitable scaffold for these cells. The findings may be useful for detailed researches on seeded cells, scaffold and implanting fashion of bone tissue engineering.
Guo
Z
Z
Department of Orthopaedics, Third Teaching Hospital, Beijing Medical University, Beijing 100083.
Dang
G
G
Wang
Z
Z
Zhang
H
H
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D006882:Hydroxyapatites; D008297:Male; D010012:Osteogenesis; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D006882:Hydroxyapatites