Novel cross-linked polyacrylamide matrices: an investigation using gradient gel electrophoresis.
Gradient gel electrophoresis was used to examine the separation properties of novel cross-linking compounds for polyacrylamide (PAAm). At low %T and at the same %C protein migration difference is accentuated for bismethacrylamide cross-linked networks relative to bisacrylamide cross-linked networks. Similar properties were observed for cyclic monomers at low %T. This trend is maintained throughout the gradient. However, at higher %T migration differential relative to N,N'-methylenebisacrylamide (Bis) was less pronounced. Evidence from gradient gels suggests that reactivity and functionality of vinyl groups impose an overriding control over network formation.
Caulfield
M J
MJ
Polymer Science Group, Department of Chemical Engineering, University of Melbourne, Victoria, Australia.
Purss
H K
HK
Solomon
D H
DH
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification
D011506:Proteins