Preparative separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from crude alkaloids of sample Aconitum sinomontanum Nakai by high-speed counter-current chromatography.
Analytical high-speed counter-current chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate alkaloids from Aconitum sinomontanum Nakai. The optimum solvent systems CHCl3-MeOH-0.3 M/0.2 M HCl (4:1.5:2, v/v) thus obtained led to the successful separation of lappaconitine, ranaconitine, N-deacetyllappaconitine and N-deacetylranaconitine from 60 to 500 mg of crude alkaloid sample by preparative HSCCC separation.
Yang
Fuquan
F
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1676, USA.
Ito
Yoichiro
Y
D000157:Aconitine / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D000470:Alkaloids / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D029626:Ranunculaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D000470:Alkaloids; C460259:N-deacetylranaconitine; C055260:N-deacetyllappaconitine; D000157:Aconitine; C022150:lappaconitine