[E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma].
OBJECTIVE
To study the significance of E-selectin and its ligand-sLeX in the metastasis of hepatocellular carcinoma (HCC).


METHODS
Flow cytometry and immunohistochemistry were used to detect the expression of E-selectin and its ligand-sLeX in HCC cell lines and in human HCC tissues.


RESULTS
The positive rate of E-selectin in vascular endothelial cells adjacent to cancer nest in tumors was 67.9% (19/28). In tumors accompanied with emboli or satellite foci, it was significantly higher than that without emboli or satellite foci (P < 0.05). The positive rate of E-selectin was not related to tumor size, tumor capsule, AFP, and the degree of differentiation. The positive expressions of sLeX in SMMU-7721, PLC/PRF/5 and HepGII cell lines were 7.03%, 63.35% and 97.29% respectively. The positive cells of sLeX were mainly distributed in the margin of tumors; the positive expression of sLeX in HCC cells in emboli or invasive tumor tissues was much higher than that in primary foci.


CONCLUSIONS
E-selectin and its ligand-sLeX are closely correlated with the metastasis of HCC.
Zhang
B
B
Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, People's Liberation Army, Shanghai 200438, China.
Chen
H
H
Yao
X
X
Cong
W
W
Wu
M
M
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D019040:E-Selectin / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009844:Oligosaccharides / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
D019040:E-Selectin; D008024:Ligands; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen