Dipyridamole-induced increase in production of rat dopaminergic neurons from mesencephalic precursors.
Dipyridamole (DIP) was tested for its ability to induce dopaminergic (DA) phenotype in cultures from epidermal growth factor-derived mesencephalic precursor cells. When these cells were incubated in media containing serum, the DA phenotype was rarely expressed. The addition of DIP increased (about 350%) the number of DA cells per neurosphere. Treatment with interleukin-1 alpha also induced a significant increase (about 300%) in the number of tyrosine hydroxylase-positive cells. However, the mixture of the most effective doses of these compounds did not induce a further increase in the number of DA cells. The results suggest that DIP may contribute to more efficient production of DA neurons for transplantation therapies in neurodegenerative diseases, and that this may be related to an enhancement of generation and/or survival of DA cells.
Rodríguez-Pallares
Jannette
J
Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Caruncho
Héctor J
HJ
Guerra
María José
MJ
Labandeira-García
José Luis
JL
D000818:Animals; D016380:Brain Tissue Transplantation / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004176:Dipyridamole / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005333:Fetus; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000494:pharmacology; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010300:Parkinson Disease / Q000628:therapy; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D007375:Interleukin-1; D010726:Phosphodiesterase Inhibitors; D004176:Dipyridamole; D014446:Tyrosine 3-Monooxygenase