title: Urinary levels of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8), and renal injuries in patients with type 2 diabetic nephropathy.
abstract: We examined the correlation among the levels of urinary monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8), hyperglycemia, and renal injuries in patients with type 2 diabetic nephropathy. The levels of urinary MCP-1, IL-8, protein excretion, blood urea nitrogen (BUN), serum creatinine (s-Cr), glycohemoglobin A1c (HbA1c), and fasting plasma glucose (FPG) were measured in 24 patients with type 2 diabetic nephropathy and 14 healthy adults as controls. Diabetic nephropathy was classified into three stages: stage 1 = normoalbuminuric, stage 2 = microalbuminuric, and stage 3 = macroalbuminuric. All of the patients showed normal ranges in renal function tests. Levels of urinary MCP-1 in all patients with diabetic nephropathy were significantly higher than those in healthy adults (P < 0.05). The levels of urinary MCP-1 in patients with diabetic nephropathy increased gradually according to the clinical stage of this disease. In contrast, the levels of urinary IL-8 in patients with diabetic nephropathy increased in stages 2 and 3. There was a significant correlation between the levels of urinary IL-8 and those of HbA1c. High glucose may stimulate MCP-1 and/or IL-8 production and their excretion into the urine independently of the phases or pathological lesions of this disease. It appears that IL-8 increased in the early stage of diabetic nephropathy, and MCP-1 increased in the advanced stage of this disease. It was concluded that measurement of urinary MCP-1 and IL-8 may be useful for evaluating the degree of renal injuries in patients with type 2 diabetic nephropathy.
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Kyouichi
authors.initials: K
authors.lastname: Tashiro
authors.forename: Ichiro
authors.initials: I
authors.lastname: Koyanagi
authors.forename: Akemi
authors.initials: A
authors.lastname: Saitoh
authors.forename: Ayumi
authors.initials: A
authors.lastname: Shimizu
authors.forename: Toshihide
authors.initials: T
authors.lastname: Shike
authors.forename: Chizuru
authors.initials: C
authors.lastname: Ishiguro
authors.forename: Michiko
authors.initials: M
authors.lastname: Koizumi
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Funabiki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Horikoshi
authors.forename: Isao
authors.initials: I
authors.lastname: Shirato
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tomino
mesh_terms: D000704:Analysis of Variance; D001786:Blood Glucose; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000652:urine*; D003928:Diabetic Nephropathies / Q000652:urine*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016209:Interleukin-8 / Q000652:urine*
chemical_list: D001786:Blood Glucose; D018932:Chemokine CCL2; D016209:Interleukin-8