title: Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells.
abstract: Recently, we investigated the inhibitory effects of 17 beta-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.
authors.affiliation: Kyoritsu College of Pharmacy, Shibakoen 1-chome, Minato-ku, Tokyo 105-8512, Japan. Oda-ti@kyoritsu-ph.ac.jp
authors.forename: Taiko
authors.initials: T
authors.lastname: Oda
authors.forename: Noriko
authors.initials: N
authors.lastname: Ikeda
authors.forename: Shoko
authors.initials: S
authors.lastname: Nakashima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sato
mesh_terms: D000077584:2-Methoxyestradiol; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004855:Equilenin / Q000737:chemistry / Q000633:toxicity*; D004857:Equilin / Q000737:chemistry / Q000633:toxicity*; D004958:Estradiol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity*; D004970:Estrone / Q000633:toxicity; D013329:Structure-Activity Relationship; D018675:Toxicity Tests
chemical_list: D004857:Equilin; D004970:Estrone; D004958:Estradiol; D000077584:2-Methoxyestradiol; D004855:Equilenin