[Apoptosis increased by bcl-2 antisense ODN in MCF-7 cell line].
OBJECTIVE
To study the effects of bcl-2 antisense oligonucleotides (ODN) on the expression of bcl-2 and apoptosis in MCF-7 cell line.


METHODS
Artificially synthesized bcl-2 specific antisense OND was introduced into MCF-7 cells by gene pulser electroprotocol. Fluorescence microscope, electron microscope, immunocytochemical staining assay, and FCM were used to detect the effects on cell apoptosis, expressional level of bcl-2 protein and cell cycle after transfecting bcl-2 antisense ODN.


RESULTS
The number of apoptotic cells was increased and expression of bcl-2 protein was declined (P < 0.05). FCM showed that the number of G(0)-G(1) phase cells was decreased and the number of G(2)-M, S phase cells were increased.


CONCLUSIONS
The effective transient expression of bcl-2 antisense ODN can be obtained by gene pulser electroprotocol in MCF-7 cell, the function of bcl-2 gene was declined and the number of apoptotic cells was increased after transfecting bcl-2 antisense ODN. bcl-2 may be used as an useful target in tumor research.
Zheng
J
J
Department of General Surgery, Clinical College, Chongqing Medical University, Chongqing 400016.
Yao
Z
Z
D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D005260:Female; D019254:Genes, bcl-2; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000235:genetics*
D020319:Oligodeoxyribonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2