title: Elliptically polarized magnetic fields do not alter immediate early response genes expression levels in human glioblastoma cells.
abstract: Expression of immediate early response genes such as c-fos, c-jun, and c-myc in response to 1-500 microT resultant (r) 60 Hz elliptically polarized (EP) magnetic fields (MFs), typical of environmental MFs polarization under overhead power lines, was analyzed in both at transcriptional and translational levels using human glioblastoma (T98G) cells. Pseudo synchronized T98G cells at G1 phase were exposed to EP-MFs (1, 20, 100, and 500 microTr) for up to 3 h, but produced no statistical difference (P>0.05) in the levels of expression ratio at both the transcriptional and translational levels at 30 min for c-fos and c-jun and at 180 min for c-myc after serum stimulation. In addition, exposure of T98G cells to linearly (vertical and horizontal) and/or circularly polarized MFs (500 microTr) produced no significant change (P>0.05) in the expression ratio at both transcriptional and post-transcriptional levels. Thus, there was no evidence that linearly or rotating polarized MFs enhanced early response gene expression in these studies. These results suggest that environmental MFs at 1-500 microT flux density are unlikely to induce carcinogenesis through a mechanism involving altered expression of the immediate early response genes.
authors.affiliation: Technical Research Center, The Kansai Electric Power Company, Inc., Hyogo, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yomori
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Yasunaga
authors.forename: Chie
authors.initials: C
authors.lastname: Takahashi
authors.forename: Ayako
authors.initials: A
authors.lastname: Tanaka
authors.forename: Shinji
authors.initials: S
authors.lastname: Takashima
authors.forename: Masaru
authors.initials: M
authors.lastname: Sekijima
mesh_terms: D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000235:genetics*; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects*; D016762:Genes, fos / Q000235:genetics / Q000528:radiation effects; D016758:Genes, jun / Q000235:genetics / Q000528:radiation effects; D016259:Genes, myc / Q000235:genetics / Q000528:radiation effects; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D012323:RNA Processing, Post-Transcriptional / Q000528:radiation effects; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000528:radiation effects*; D014407:Tumor Cells, Cultured
chemical_list: D012335:RNA, Ribosomal