Determination of some anti-human immunodeficiency virus nucleosides by capillary zone electrophoresis-tandem mass spectrometry.
We describe the analysis of two potent anti-human immunodeficiency virus reverse transcriptase (RT-HIV) inhibitors, zidovudine (AZT) and stavudine (d4T), among a pool of natural nucleosides (A, C, G, U, T) by capillary zone electrophoresis (CZE)-ionspray-tandem mass spectrometry (MS/MS) in positive ion mode. Several volatile formic acid-ammonia buffers having the same ionic strength (50 mM) but different pH values varying in the 9-11 pH range were prepared and tested to determine the best electrophoretic migration conditions. Quantitative CZE-MS/MS analysis was performed using selected reaction monitoring (SRM) mode. Finally, this CZE-MS/MS procedure opens the possibility for future determination of several nucleoside RT-HIV inhibitors in cell pool samples.
Cahours
Xavier
X
Institut de Chimie Organique et Analytique, CNRS UMR 6005, Orleans, France.
Morin
Philippe
P
Dessans
Helene
H
Agrofoglio
Luigi A
LA
D019380:Anti-HIV Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards*; D006863:Hydrogen-Ion Concentration; D009705:Nucleosides / Q000032:analysis*; D009994:Osmolar Concentration; D012015:Reference Standards; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards; D018119:Stavudine / Q000032:analysis; D015215:Zidovudine / Q000032:analysis
D019380:Anti-HIV Agents; D009705:Nucleosides; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D018119:Stavudine