Transcription of the Drosophila CKI gene dacapo is regulated by a modular array of cis-regulatory sequences.
Organogenesis requires coordination between developmental programs and cell cycle progression. The Cip/Kip families of cyclin-dependent kinase inhibitor (CKI) are main effectors linking these two programs. In many instances, expression of Cip/Kip CKIs are increased abruptly in cells entering their last mitotic cycle, suggesting that CKI expression is developmentally regulated. Expression of Dacapo (Dap), a Drosophila Cip/Kip CKI, is transiently up-regulated immediately before cells enter G1 arrest. Here we report that dap transcription is controlled by modular arrays of tissues specific cis-regulatory elements. Furthermore, we identified pan-neural Prospero as a regulator of dap transcription in the developing nervous system, providing an example how tissue-specific developmental programs can be linked to cell cycle progression.
Liu
Te-Hui
TH
Department of Molecular Genetics, Neurobiotechnology Center, Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
Li
Ling
L
Vaessin
Harald
H
D000818:Animals; D002453:Cell Cycle; D002490:Central Nervous System / Q000196:embryology; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D004817:Epidermis / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000196:embryology; D014158:Transcription, Genetic
D029721:Drosophila Proteins; D009687:Nuclear Proteins; C103336:dap protein, Drosophila