A proteome database of human primary T helper cells.
We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4+ human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [35S]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROTand NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.
Nyman
T A
TA
Centre for Biotechnology, University of Turku and Abo Akademi University, Finland. tuula.nyman@btk.utu.fi
Rosengren
A
A
Syyrakki
S
S
Pellinen
T P
TP
Rautajoki
K
K
Lahesmaa
R
R
D030562:Databases, Protein; D006801:Humans; D020543:Proteome; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D020543:Proteome