title: Reversed-phase liquid chromatographic determination of phytometallophores from Strategy II Fe-uptake species by 9-fluorenylmethyl chloroformate fluorescence.
abstract: An HPLC method to quantitate phytometallophores (phytosiderophores) exuded from roots of barley (Hordeum vulgare L.) growing in nutrient solution culture was developed. 9-Fluorenylmethyl chloroformate (FMOC) derivatives of phytometallophores were separated on a C18 reverse-phase column using a sodium acetate (pH 7.2) and acetonitrile-methanol gradient over 20 min followed by fluorescence detection. Detection limits ranged from 15 to 370 pmol depending on the particular phytometallophore. The effectiveness of this method was demonstrated using the response of barley seedlings to Fe-sufficient and Fe-deficient nutrient solution conditions. Phytometallophores collected in root washings of Fe-deficient barley seedlings increased with plant age while phytometallophore release from Fe-adequate roots was negligible.
authors.affiliation: Department of Crop and Soil Sciences, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Matthew S
authors.initials: MS
authors.lastname: Wheal
authors.forename: Laurence I
authors.initials: LI
authors.lastname: Heller
authors.forename: Wendell A
authors.initials: WA
authors.lastname: Norvell
authors.forename: Ross M
authors.initials: RM
authors.lastname: Welch
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry*; D001467:Hordeum / Q000737:chemistry / Q000196:embryology; D012639:Seeds / Q000737:chemistry; D017262:Siderophores / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005449:Fluorenes; D017262:Siderophores; C054007:1-(9-fluorenyl)methyl chloroformate