title: Necrotic seminoma of the testis: establishing the diagnosis with Masson trichrome stain and immunostains.
abstract: We describe an infarcted mass in the testis containing "ghost" cells suspicious for neoplasm. The entire lesion was necrotic. A Masson trichrome stain greatly improved nuclear and cytologic detail, confirming the suspicion of neoplasm. Placental alkaline phosphatase revealed specific membrane staining of the neoplastic cells and established a diagnosis of seminoma. Masson trichrome plus selected immunostains offer a promising approach to the diagnosis of certain necrotic neoplasms.
authors.affiliation: Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Florentine
authors.forename: Arno A
authors.initials: AA
authors.lastname: Roscher
authors.forename: Jerry
authors.initials: J
authors.lastname: Garrett
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Warner
mesh_terms: D006801:Humans; D008297:Male; D008875:Middle Aged; D009336:Necrosis; D018239:Seminoma / Q000175:diagnosis / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*; D013736:Testicular Neoplasms / Q000175:diagnosis / Q000473:pathology*