Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray.
Genes differentially expressed between parthenogenetic and androgenetic embryos are candidates for the identification of imprinted genes, which are expressed specifically from the maternal or paternal allele. To search for genes differentially expressed between parthenogenetic and androgenetic embryos, we used the RIKEN full-length enriched mouse cDNA microarray. The 25 candidates obtained included 8 known imprinted genes (such as IgfII, Snrpn, and Neuronatin) and 3 new ones--Asb4 (ankyrin repeat and SOCS box-containing protein 4), Ata3 (amino acid transport system A3), and Decorin--which were confirmed by using normal diploid embryos from the reciprocal F1 crosses of B6 and JF1 mice. The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray.
Mizuno
Yosuke
Y
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230-0045, Japan.
Sotomaru
Yusuke
Y
Katsuzawa
Yukiko
Y
Kono
Tomohiro
T
Meguro
Makiko
M
Oshimura
Mitsuo
M
Kawai
Jun
J
Tomaru
Yasuhiro
Y
Kiyosawa
Hidenori
H
Nikaido
Itoshi
I
Amanuma
Hiroshi
H
Hayashizaki
Yoshihide
Y
Okazaki
Yasushi
Y
D026924:Amino Acid Transport System A / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics*; D001324:Autoantigens / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D058575:Decorin; D004622:Embryo, Mammalian; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D005820:Genetic Testing / Q000379:methods*; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
D026924:Amino Acid Transport System A; D001324:Autoantigens; D002352:Carrier Proteins; C546879:Dcn protein, mouse; C546880:Dcn protein, rat; D058575:Decorin; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; D011509:Proteoglycans; D017411:Ribonucleoproteins, Small Nuclear; C420522:Slc38a4 protein, mouse; C486010:Slc38a4 protein, rat; D055517:snRNP Core Proteins; D007335:Insulin-Like Growth Factor II