[Association of tumor necrosis factor microsatellite polymorphism with chronic atrophic gastritis and gastric adenocarcinoma].
OBJECTIVE
To investigate whether TNFd and TNFe microsatellite polymorphisms correlate with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population.


METHODS
In 164 healthy controls, 53 patients with chronic atrophic gastritis, and 56 patients with gastric adenocarcinoma, TNFd and TNFe microsatellite alleles were typed using PCR technique, followed by high-voltage denaturing PAGE, with silver staining. At the same time, the PCR products were cloned and sequenced.


RESULTS
Fight alleles at the TNFd locus and four alleles at the TNFe locus were detected. A new allele was found and identified, its accession number of Genebank is AF315593. Statistical analysis showed that there existed a very strong linkage equilibrium between TNFd8 and TNFe4 allele. No difference was observed between patients and healthy controls in the distribution of alleles at the TNFd and TNFe loci (P > 0.05). The frequence of d2/d6 genotype was significantly higher in patients with gastric adenocarcinoma than in healthy individuals (P = 0.028). d2/d6 genotype wasn't related to age or gender or grade of differentiation or clinicopathologic stage in gastric adenocarcinoma.


CONCLUSIONS
d8e4 haplotype appears to be specific of Chinese population. d2/d6 genotype is associated with the susceptibility to gastric adenocarcinoma.
Fei
B
B
Gastroenterology Department, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
Deng
C
C
Xia
B
B
D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D005260:Female; D005757:Gastritis, Atrophic / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013274:Stomach Neoplasms / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha