[Co-transplantation of islet and allogeneic testicular cell induced immune privilege].
OBJECTIVE
To induce islet allograft long-term survival through co-transplantation islet with sertoli cell.


METHODS
Testicular sertoli cell was prepared by digestion of collagenase, trypsin and Dnase, and cultured for 48 hours. Purified donor (Wistar rat) islet was co-transplanted with allergic sertoli cell, in the absence of systemic immunosuppression. TUNEL (terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling) was used to label the apoptosis of lymphocytes surrounding the islet graft. The SABC method was used to label the expression of Fas ligand in co-transplantation.


RESULTS
Islet co-transplantation with 1 x 10(7) sertoli cell reversed the diabetic state for more than 60 days in 100% (6/6) of the chemically diabetic SD recipients. Similar grafts consisting of islet alone or islet plus 1 x 10(5)-sertoli cell survived only for 5 - 6 days. Apoptosis of the lymphocytes surrounding the islet was quite clear.


CONCLUSIONS
Co-transplantation islet with FasL(+) sertoli cell induces local immune privilege and allows long-term graft survival without systemic immunosuppression.
Lan
P
P
Department of Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041.
Yan
L
L
Zhan
W
W
Xiao
L
L
D000818:Animals; D017209:Apoptosis; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000601:surgery; D053222:Fas Ligand Protein; D006085:Graft Survival; D007108:Immune Tolerance; D016381:Islets of Langerhans Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000637:transplantation*; D048069:Tumor Necrosis Factors / Q000378:metabolism
D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D048069:Tumor Necrosis Factors