title: [Molecular cloning of specific porcine adipocyte membrane protein cDNA].
abstract: Specific porcine adipocyte membrane protein (PAMP), which was recently reported, is probably associated with the growth and development of adipocyte and regulator of adipose tissue. Using RT-PCR and RACE methods, and primer designed according to published sequence of PAMP gene 5' end, we have successfully amplified about a 500 bp and a 400 bp from pig adipose tissue. A 500 bp fragment was retrieved, cloned and sequenced. The sequencing result showed that we have got the clone and sequence of cDNA 5'-end for PAMP gene.
authors.affiliation: College of Animal Husbandry and Veterinary, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ju-Qiang
authors.initials: JQ
authors.lastname: Lin
authors.forename: Si-Wen
authors.initials: SW
authors.lastname: Jiang
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.forename: Yuan-Zhu
authors.initials: YZ
authors.lastname: Xiong
mesh_terms: D017667:Adipocytes / Q000502:physiology*; D000273:Adipose Tissue / Q000254:growth & development*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins