title: The human T-type amino acid transporter-1: characterization, gene organization, and chromosomal location.
abstract: System T is a Na+-independent transport system that selectively transports aromatic amino acids. Here, we determined the structure of the human T-type amino-acid transporter-1 (TAT1) cDNA and gene (SLC16A10). The human TAT1 cDNA encoded a 515-amino-acid protein with 12 putative membrane-spanning domains. Human SLC16A10 was localized on human chromosome 6, mapped to 6q21-q22. SLC16A10 contains six exons spanning 136 kb. In contrast to rat TAT1, which is mainly present in the intestine, human TAT1 was strongly expressed in human kidney as well as in human intestine. Expression of human TAT1 in Xenopus laevis oocytes demonstrated the Na+-independent transport of tryptophan, tyrosine, phenylalanine, and L-dopa, indicating that human TAT1 is a transporter subserving system T. Because human TAT1 is proposed to be crucial to the efficient absorption of aromatic amino acids from intestine and kidney, its defect could be involved in the disruption of aromatic amino-acid transport, such as in blue diaper syndrome.
authors.affiliation: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka, Tokyo 181-8611, Japan.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Kanai
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsuo
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Arthit
authors.initials: A
authors.lastname: Chairoungdua
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Enomoto
authors.forename: Seok Ho
authors.initials: SH
authors.lastname: Cha
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Goya
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Endou
mesh_terms: D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D006801:Humans; D008969:Molecular Sequence Data; D009865:Oocytes; D051381:Rats; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014982:Xenopus laevis
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; C498964:SLC16A10 protein, human; C429535:Slc16a10 protein, rat