Comparative distribution of short dystrophin superfamily products in various guinea pig spermatozoa domains.
In this study, the presence and cellular distribution of dystrophin family products (i.e. Dp71d, Dp71f-like protein and dystrobrevin) was examined by indirect immunofluorescence and Western blotting in guinea pig spermatozoa. Two dystrophin-associated proteins, beta-dystroglycan and alpha-syntrophin, and nNOS a protein frequently associated with alpha-syntrophin, were determined. In spermatozoa lacking plasma membrane and acrosome, Dp71f-like protein was found in the postacrosomal perinuclear theca and also in the middle piece of the flagellum. In the flagellum, Dp71f-like protein is localized together with alpha-syntrophin and nNOS. Dp71d was present in the plasma membrane of the middle piece with beta-dystroglycan, alpha-syntrophin and nNOS. Dp71d was also present in plasma membrane of the post acrosomal region, but only with nNOS. Finally, dystrobrevin was located all along skeletal flagellum structures and in the subacrosomal hemisphere of the perinuclear theca. This distinct and complementary distribution in various domains of spermatozoa may reveal a specific function for each short dystrophin family product, in the stabilization of the domains where they are located.
Hernández-González
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Dept of Cell Biology, CINVESTAV-IPN, Col. San Pedro Zacatenco, DF, México. eoton@cell.cinvestav.mx
Martínez-Rojas
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Mornet
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Rendon
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Mújica
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D000818:Animals; D002462:Cell Membrane / Q000235:genetics / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000032:analysis; D049030:Dystroglycans; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D049029:Dystrophin-Associated Proteins; D006168:Guinea Pigs; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D052248:Nitric Oxide Synthase Type I; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; C111539:DTNB protein, human; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D009479:Neuropeptides; C085339:apo-dystrophin 1; C091824:syntrophin; D049030:Dystroglycans; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I