Arabidopsis seedling growth, storage lipid mobilization, and photosynthetic gene expression are regulated by carbon:nitrogen availability.
The objective of the current work was to establish the degree to which the effects of carbon and nitrogen availability on Arabidopsis seedling growth and development are due to these nutrients acting independently or together. Growth of seedlings on low (0.1 mM) nitrogen results in a significant reduction of seedling and cotyledon size, fresh weight, chlorophyll, and anthocyanin content but a slight increase in endogenous sugars. The addition of 100 mM sucrose (Suc) to the nitrogen-depleted growth media results in a further reduction in cotyledon size and chlorophyll content and an overall increase in anthocyanins and endogenous sugars. Storage lipid breakdown is almost completely blocked in seedlings grown on low nitrogen and 100 mM Suc and is significantly inhibited when seedlings are grown on either low nitrogen or high Suc. Carbohydrate repression of photosynthetic gene expression can only be observed under low nitrogen conditions. Low (0.1 mM) nitrogen in the absence of exogenous carbohydrate results in a significant decrease in chlorophyll a/b-binding protein and ribulose bisphosphate carboxylase small subunit gene transcript levels. Thus, carbon to nitrogen ratio rather than carbohydrate status alone appears to play the predominant role in regulating various aspects of seedling growth including storage reserve mobilization and photosynthetic gene expression.
Martin
Thomas
T
Plant Molecular Science Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
Oswald
Oliver
O
Graham
Ian A
IA
D000872:Anthocyanins / Q000378:metabolism; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004537:Eicosanoic Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D045342:Light-Harvesting Protein Complexes; D050356:Lipid Metabolism; D009584:Nitrogen / Q000494:pharmacology*; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics*; D063245:Plant Development; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D010944:Plants / Q000187:drug effects / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D015398:Signal Transduction; D013395:Sucrose / Q000494:pharmacology*; D014280:Triglycerides / Q000378:metabolism
D000872:Anthocyanins; D029681:Arabidopsis Proteins; C423618:CAB2 protein, Arabidopsis; D002352:Carrier Proteins; D004537:Eicosanoic Acids; D045342:Light-Harvesting Protein Complexes; D014280:Triglycerides; D002734:Chlorophyll; D013395:Sucrose; D012273:Ribulose-Bisphosphate Carboxylase; D005947:Glucose; D009584:Nitrogen