Molecular cloning of SLC26A7, a novel member of the SLC26 sulfate/anion transporter family, from high endothelial venules and kidney.
A unique characteristic of endothelial cells from high endothelial venules (HEVEC) in lymphoid organs and chronically inflamed tissues is their capacity to incorporate large amounts of sulfate into sialomucin-type counter-receptors for the lymphocyte homing receptor L-selectin. We have previously shown that HEVEC express two functional classes of sulfate transporters: sodium/sulfate cotransporters and sulfate/anion exchangers. Here, we report the molecular cloning from human HEVEC of a 2.9-kb cDNA encoding SLC26A7, a novel member of the SLC26 (solute carrier 26) sulfate/anion exchanger family. SLC26A7 exhibits 30% identity with three known sulfate transporters from the SLC26 family: SLC26A2 (also known as DTDST), SLC26A1 (also known as SAT1), and SLC26A3 (also known as DRA). Northern blot analysis revealed specific expression of SLC26A7 mRNA in kidney. Alternative splicing and polyadenylation of SLC26A7 pre-mRNA in kidney suggest the existence of two protein isoforms, SLC26A7.1 and SLC26A7.2, differing in their carboxy termini.
Vincourt
Jean-Baptiste
JB
Laboratoire de Biologie Vasculaire, Institut de Pharmacologie et de Biologie Structurale du CNRS, 205 route de Narbonne, Toulouse, 31077, France.
Jullien
Denis
D
Kossida
Sophia
S
Amalric
François
F
Girard
Jean-Philippe
JP
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000032:analysis / Q000235:genetics*; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D027963:Chloride-Bicarbonate Antiporters; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004730:Endothelium, Vascular / Q000737:chemistry*; D006801:Humans; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D017385:Sequence Homology; D000076988:Sulfate Transporters; D014018:Tissue Distribution; D014699:Venules / Q000737:chemistry*
D017920:Antiporters; D002352:Carrier Proteins; D027963:Chloride-Bicarbonate Antiporters; D018076:DNA, Complementary; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C085609:SLC26A4 protein, human; C455626:SLC26A7 protein, human; C486743:Slc26a7 protein, mouse; D000076988:Sulfate Transporters