title: Influence of physico-chemical parameters of some barbituric acid derivatives on their retention on an amide embedded RP silica column.
abstract: Retention parameters of 45 different barbituric acid derivatives were determined on an amide embedded RP silica column (Discovery RP-AmideC16) using non-buffered water-acetonitrile eluent systems. Linear correlation were calculated between the logarithm of the capacity factor and the acetonitrile concentration in the eluent. To determine the retention behavior of barbituric acid derivatives, stepwise regression analysis (SRA) and principal component analysis (PCA) followed by two-dimensional nonlinear and modified nonlinear mapping was used. It can be concluded, the retention of barbituric acid derivatives are governed mainly by the steric parameters of the substituents. Principal component analysis indicated that the barbituric acid derivatives have mixed retention on this amide embedded RP silica column in water-acetonitrile eluent.
authors.affiliation: Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, PO Box 17, 1525, Budapest, Hungary. janna@chemres.hu
authors.forename: Annamaria
authors.initials: A
authors.lastname: Jakab
authors.forename: Miklos
authors.initials: M
authors.lastname: Prodan
authors.forename: Esther
authors.initials: E
authors.lastname: Forgács
mesh_terms: D001463:Barbiturates / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid
chemical_list: D001463:Barbiturates; C032232:barbituric acid