A quantitative analysis of the kinetics of Gal4 activator and effector gene expression in the zebrafish.
Using a temperature-inducible hsp70:Gal4 activator and UAS:myc-notch1a-intra as effector, we determined quantitatively the kinetics of expression of both transgenes and analysed the effects of varying their expressivity on several phenotypic traits in the developing zebrafish. hsp70:Gal4 is transcribed within 15 min after temperature-mediated induction, but Gal4 RNA decays rapidly. The Gal4 protein was found to be quite stable, as functional Gal4, which was detectable 1.5 h after heat shock (HS), persisted for at least 13 h. myc-notch1a-intra RNA is expressed approximately 1.5 h after HS, but unlike the Gal4 RNA, it was found to be very stable; it continues to accumulate during the succeeding 17 h after HS. Fully penetrant phenotypic effects are obtained after a relatively long activator induction with a 30-min HS.
Scheer
Nico
N
Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Köln, Germany.
Riedl
Iris
I
Warren
J T
JT
Kuwada
John Y
JY
Campos-Ortega
José A
JA
D000818:Animals; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006358:Hot Temperature; D017403:In Situ Hybridization; D007700:Kinetics; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D013997:Time Factors; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D015027:Zebrafish
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012313:RNA