Cloning and expression of a full-length cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase B.
Inositol 1,4,5-trisphosphate (InsP(3)) 3-kinase catalyzes the phosphorylation of InsP(3) to inositol 1,3,4,5-tetrakisphosphate (InsP(4)). cDNAs encoding three isoenzymes of InsP(3) 3-kinase (3-kinases A, B, and C) have been previously reported; however, a demonstrably full-length cDNA encoding human InsP(3) 3-kinase B was still lacking. Here we report the cloning of a full-length 2841-bp cDNA encoding human InsP(3) 3-kinase B. Northern blot analysis shows the presence of an ubiquitous transcript of approximately 7.2 kb in a large number of human tissues. InsP(3) 3-kinase activity measured in COS-7 cells transfected with InsP(3) 3-kinase B shows an activity that was 8-fold increased upon the addition of Ca(2+)/calmodulin in the assay mixture.
Dewaste
Valérie
V
Interdisciplinary Research Institute (IRIBHN), Université Libre de Bruxelles, Campus Erasme, 808 Route de Lennik, Brussels, Belgium.
Roymans
Dirk
D
Moreau
Colette
C
Erneux
Christophe
C
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase; D002118:Calcium