title: Preparation of an active recombinant peptide of crustacean androgenic gland hormone.
abstract: In crustaceans, male sexual characteristics are induced by a hormone referred to as androgenic gland hormone. We have recently cloned a candidate cDNA in the terrestrial isopod Armadillidium vulgare. In order to prove that this cDNA encodes the hormone, recombinant single-chain precursor molecules consisting of B chain, C peptide and A chain were produced using both baculovirus and bacterial expression systems. Neither recombinant precursors showed activity. Digestion of only the precursor carrying a glycan moiety with lysyl endopeptidase gave a heterodimeric peptide with hormonal activity by removing a part of C peptide. These results indicate that the cDNA encodes the hormone.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan.
authors.forename: Atsuro
authors.initials: A
authors.lastname: Okuno
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ohira
authors.forename: Rinkei
authors.initials: R
authors.lastname: Ko
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kurihara
authors.forename: Shogo
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003445:Crustacea / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism
chemical_list: D004220:Disulfides; D042341:Gonadal Hormones; D012739:Gonadal Steroid Hormones; D011134:Polysaccharides; D011994:Recombinant Proteins; C053644:androgenic gland hormone (Armadillidium)