title: Comparative study of enterostatin sequence in five rat strains and enterostatin binding proteins in rat and chicken serum.
abstract: Enterostatin, a pentapeptide derived from the precursor protein procolipase has been shown to inhibit dietary fat intake and to reduce body fat after chronic administration in rats. We repeat that the enterostatin amino acid sequence from the genomic DNA of 5 different rat strains is APGPR. 125I-APGPR bound to three proteins (300, 205 and 60 kDa) in rat serum and one 60 kDa protein in chicken serum. These serum binding proteins were also eluted by APGPR affinity chromatography. Western blot analysis of serum protein identified enterostatin-like immunoreactivity associated with the same molecular weight bands. Our results demonstrate the enterostatin sequence in rat is APGPR and suggest the presence of enterostatin binding proteins in rat and chicken serum.
authors.affiliation: Experimental Obesity Laboratory, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA.
authors.forename: Yingjen Jeffrey
authors.initials: YJ
authors.lastname: Wu
authors.forename: David
authors.initials: D
authors.lastname: Hughes
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.forename: Doug H
authors.initials: DH
authors.lastname: Braymer
authors.forename: David A
authors.initials: DA
authors.lastname: York
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002645:Chickens; D002846:Chromatography, Affinity; D003089:Colipases / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004792:Enzyme Precursors; D016678:Genome; D007457:Iodine Radioisotopes; D008297:Male; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D011924:Rats, Zucker
chemical_list: D001798:Blood Proteins; D003089:Colipases; D004792:Enzyme Precursors; D007457:Iodine Radioisotopes; D011498:Protein Precursors; C037107:procolipase; D004247:DNA