title: Effect of a regulatory mutation on the rat atrial natriuretic peptide gene transcription.
abstract: To investigate the functional relevance of a regulatory mutation affecting the enhancer element PEA2 of the rat ANP gene we transfected rat cardiomyocytes and aortic endothelial cells with either the mutant or the wild-type ANP promoter construct (-683 +54) and performed CAT assays both at baseline and in response to Phenylephrine and Angiotensin II. In the myocardial cells we also determined the DNA/nuclear protein interaction through electrophoretic mobility shift assay. These studies showed a significantly lower degree of ANP transcription in the presence of the mutant PEA2 site, thus demonstrating its functional significance and the biological relevance of ANP gene structural alterations.
authors.affiliation: Istituto Neuromed, Polo Molisano dell'Universita' La Sapienza di Roma, Pozzilli, Italy. rubattu.speranza@neuromed.it
authors.forename: Speranza
authors.initials: S
authors.lastname: Rubattu
authors.forename: Rosangela
authors.initials: R
authors.lastname: Giliberti
authors.forename: Paola
authors.initials: P
authors.lastname: De Paolis
authors.forename: Rosita
authors.initials: R
authors.lastname: Stanzione
authors.forename: Paola
authors.initials: P
authors.lastname: Spinsanti
authors.forename: Vanessa
authors.initials: V
authors.lastname: Venturelli
authors.forename: Massimo
authors.initials: M
authors.lastname: Volpe
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors; D009320:Atrial Natriuretic Factor