title: Rapid and sensitive method for the analysis of carbon monoxide in blood using gas chromatography with flame ionisation detection.
abstract: In order to measure changes in physiological CO concentrations in blood with good accuracy, a method was developed using gas chromatography with flame ionisation detection (250 degrees C). A nickel catalyst system was fitted to convert CO to methane at 375 degrees C after separation with a molecular sieve column at 35 degrees C. Helium was used as carrier at 30 ml/min. Porcine or human blood (400 microl) was sampled in gastight tubes and treated with sulfuric acid and saponin (800 microl). Accuracy was 1.4% and 1.5% (RSD), respectively. Precision was 2.8% (porcine blood). Limit of detection was 0.01 nmol/ml gas and limit of quantification 12 nmol/ml blood. Calibration was made in the interval 12-514 nmol/ml blood (corresponding to 0.1-6% COHb). Samples were stable for at least a month at +4 degrees C. This paper describes a method with high sensitivity and good accuracy, suitable for analysis of low CO concentrations.
authors.affiliation: Department of Surgery and Perioperative Sciences, Umea University Hospital, Sweden. annamaja.sundin@anestesi.umu.se
authors.forename: Anna-Maja
authors.initials: AM
authors.lastname: Sundin
authors.forename: Jan Erik
authors.initials: JE
authors.lastname: Larsson
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D002248:Carbon Monoxide