Isolation and characterization of an unusual 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase gene from Enterobacter cloacae UW4.
A genomic library of the 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing plant growth-promoting bacterium Enterobacter cloacae UW4 in pUC19 in Escherichia coli was screened for the ability to utilize ACC as a sole source of nitrogen. One of the clones that was isolated contained a plasmid with an insert of approximately 0.8 kb that conferred ACC deaminase activity. Sequence analysis revealed that this DNA fragment contains an open-reading frame of 696 nucleotides predicted to encode a protein of 232 amino acids, a member of the amidohydrolase protein superfamily, i.e., a deaminase that contains a mononuclear or binuclear metal center as compared to the canonical ACC deaminase which contains pyridoxal phosphate as a co-factor.
Li
J
J
Department of Biology, University of Waterloo, Ontario, Canada.
Shah
S
S
Moffatt
B A
BA
Glick
B R
BR
D000595:Amino Acid Sequence; D000598:Amino Acids, Cyclic / Q000378:metabolism; D001483:Base Sequence; D019755:Carbon-Carbon Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D015698:Genomic Library; D008969:Molecular Sequence Data; D063245:Plant Development; D010944:Plants / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
D000598:Amino Acids, Cyclic; D004269:DNA, Bacterial; C023863:1-aminocyclopropane-1-carboxylic acid; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases