title: Inhibition of lens epithelial cell migration by an acrylic intraocular lens in vitro.
abstract: We developed a new in vitro system to evaluate the effect of intraocular lenses (IOLs) on the migration of lens epithelial cells (LECs) and determined how acrylic and other IOLs influence LEC migration using this model. In an in vitro system, porcine LECs were cultured in a cell culture chamber insert, containing a collagen membrane, for 10 days with no IOL or with various types of IOLs. Migration of LECs beneath each IOL optic was observed with an inverted-phase microscope. The cell-free areas under the IOL optic, where the LECs had not migrated, were measured. Without IOL, LECs completely covered the collagen membrane within 5 days after plating (5.0 +/- 0.0 days). Complete coverage was slowed by a silicone IOL (6.7 +/- 1.2 days, p = 0.0305). LECs cultured with acrylic or with round- or sharp-edged polymethylmethacrylate (PMMA) IOLs did not completely cover the area. Ten days after initiating cultures, the cell-free areas under IOLs with sharp edges (acrylic, 41.1 +/- 8.0%; sharp-edged PMMA, 60.9 +/- 39.0%) were significantly larger than under IOLs with round edges (silicone, 0.0 +/- 0.0%; round-edged PMMA, 1.5 +/- 1.2%). A sharp edge may act as a barrier to LEC migration. Moreover, LEC migration under the acrylic IOL slowed after the LECs had crossed the barrier of the optic edge, perhaps due to acrylic adhesive properties. Only a few LECs reached the collagen membrane beneath the central 3 mm of the acrylic IOL. This new in vitro model was useful in evaluating the effect of various IOLs on LEC migration. Acrylic IOLs inhibited LEC migration by not only a sharp edge but also other factors, such as adhesive properties.
authors.affiliation: Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan. kurosaka@sc.itc.keio.ac.jp
authors.forename: Daijiro
authors.initials: D
authors.lastname: Kurosaka
authors.forename: Minoru
authors.initials: M
authors.lastname: Obasawa
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Kurosaka
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D002448:Cell Adhesion; D002464:Cell Migration Inhibition; D002465:Cell Movement; D004847:Epithelial Cells / Q000166:cytology*; D007908:Lens, Crystalline / Q000166:cytology*; D007910:Lenses, Intraocular; D008954:Models, Biological; D019904:Polymethyl Methacrylate; D012826:Silicone Elastomers; D013552:Swine
chemical_list: D000180:Acrylic Resins; D012826:Silicone Elastomers; D019904:Polymethyl Methacrylate