[The invasive ability of bladder cancer].
OBJECTIVE
To find a direct method for testing the invasive ability of bladder cancer.


METHODS
The invasive ability of 49 specimens of primarily cultured bladder cancer, transfected BIU-87 cells with pcDNA3-E1AF and untransfected BIU-87 cells was evaluated by Matrigel invasion assay.


RESULTS
Sixteen of the 49 specimens were found to penetrate the matrigel barrier, and the result of the Matrigel invasion was related to the grade of the tumor cells. The number of positive cells of transfected BIU-87 was remarkably larger than that of untransfected BIU-87.


CONCLUSIONS
Matrigel invasion assay is a simple, direct and dependable method, and could be used clinically to test the invasive ability of bladder cancer.
Gu
Y
Y
Department of Urology, People's Hospital, Beijing Medical University, Beijing 100044, China.
Hou
S
S
Li
Y
Y
Zhang
X
X
D017500:Adenovirus E1A Proteins / Q000235:genetics; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D014162:Transfection; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D017500:Adenovirus E1A Proteins; C079853:ETV4 protein, human; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets