Decreased DNA repair rates and protection from heat induced apoptosis mediated by electromagnetic field exposure.
In this study, we demonstrate that electromagnetic field (EMF) exposure results in protection from heat induced apoptosis in human cancer cell lines in a time dependent manner. Apoptosis protection was determined by growing HL-60, HL-60R, and Raji cell lines in a 0.15 mT 60 Hz sinusoidal EMF for time periods between 4 and 24 h. After induction of apoptosis, cells were analyzed by the neutral comet assay to determine the percentage of apoptotic cells. To discover the duration of this protection, cells were grown in the EMF for 24 h and then removed for 24 to 48 h before heat shock and neutral comet assays were performed. Our results demonstrate that EMF exposure offers significant protection from apoptosis (P<.0001 for HL-60 and HL-60R, P<.005 for Raji) after 12 h of exposure and that protection can last up to 48 h after removal from the EMF. In this study we further demonstrate the effect of the EMF on DNA repair rates. DNA repair data were gathered by exposing the same cell lines to the EMF for 24 h before damaging the exposed cells and non-exposed cells with H2O2. Cells were allowed to repair for time periods between 0 and 15 min before analysis using the alkaline comet assay. Results showed that EMF exposure significantly decreased DNA repair rates in HL-60 and HL-60R cell lines (P<.001 and P<.01 respectively), but not in the Raji cell line. Importantly, our apoptosis results show that a minimal time exposure to an EMF is needed before observed effects. This may explain previous studies showing no change in apoptosis susceptibility and repair rates when treatments and EMF exposure were administered concurrently. More research is necessary, however, before data from this in vitro study can be applied to in vivo systems.
Robison
Jacob G
JG
Department of Microbiology, Brigham Young University, Provo, Utah 84602, USA.
Pendleton
Amanda R
AR
Monson
Kirk O
KO
Murray
Byron K
BK
O'Neill
Kim L
KL
D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D002452:Cell Count; D002455:Cell Division; D004260:DNA Repair / Q000502:physiology / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D018922:HL-60 Cells; D006358:Hot Temperature; D006801:Humans; D013997:Time Factors; D014407:Tumor Cells, Cultured