[Experimental study of periosteal osteoblasts coculture with freeze-dried demineralized bone matrix].
OBJECTIVE
To investigate the feasibility of freeze-dried demineralized bone matrix (FDBM) as scaffold material in bone tissue engineering.


METHODS
Osteoblasts which were isolated from cranial periosteum of New Zealand rabbits were cultured as the seeding cells, then the cells were cocultured with heterogenous FDBM in vitro. The cell-material complex was observed under phase microscope, light microscope and electronic scanning microscope in order to evaluate the interaction between cells and FDBM.


RESULTS
Eight hours after coculture, the osteoblasts adhered to FDBM scaffolds. Seven days later, the osteoblasts differentiated and proliferated in FDBM network. Extracellular matrix was secreted and calcium nodes were formed among osteoblasts.


CONCLUSIONS
FDBM is a good scaffold material for the bone tissue engineering.
Li
Yu-biao
YB
Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu Sichuan, P. R. China, 610041.
Yang
Zhi-ming
ZM
Li
Xiu-qun
XQ
D000818:Animals; D001672:Biocompatible Materials; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D005612:Freeze Drying; D010006:Osteoblasts / Q000502:physiology*; D011817:Rabbits; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials