title: Spontaneous axonal regeneration in rodent spinal cord after ischemic injury.
abstract: Here we present evidence for spontaneous and long-lasting regeneration of CNS axons after spinal cord lesions in adult rats. The length of 200 kD neurofilament (NF)-immunolabeled axons was estimated after photochemically induced ischemic spinal cord lesions using a stereological tool. The total length of all NF-immunolabeled axons within the lesion cavities was increased 6- to 10-fold at 5, 10, and 15 wk post-lesion compared with 1 wk post-surgery. In ultrastructural studies we found the putatively regenerating axons within the lesion to be associated either with oligodendrocytes or Schwann cells, while other fibers were unmyelinated. Immunohistochemistry demonstrated that some of the regenerated fibers were tyrosine hydroxylase- or serotonin-immunoreactive, indicating a central origin. These findings suggest that there is a considerable amount of spontaneous regeneration after spinal cord lesions in rodents and that the fibers remain several months after injury. The findings of tyrosine hydroxylase- and serotonin-immunoreactivity in the axons suggest that descending central fibers contribute to this endogenous repair of ischemic spinal cord injury.
authors.affiliation: Department of NEUROTEC, Huddinge University Hospital, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: von Euler
authors.forename: A M
authors.initials: AM
authors.lastname: Janson
authors.forename: J O
authors.initials: JO
authors.lastname: Larsen
authors.forename: A
authors.initials: A
authors.lastname: Seiger
authors.forename: L
authors.initials: L
authors.lastname: Forno
authors.forename: M B
authors.initials: MB
authors.lastname: Bunge
authors.forename: E
authors.initials: E
authors.lastname: Sundström
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence; D016900:Neurofilament Proteins / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012038:Regeneration / Q000502:physiology*; D012583:Schwann Cells / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D013119:Spinal Cord Injuries / Q000503:physiopathology*
chemical_list: D016900:Neurofilament Proteins; C051774:neurofilament protein H; D012701:Serotonin