title: Evaluation of bedmaking-related airborne and surface methicillin-resistant Staphylococcus aureus contamination.
abstract: The number of airborne methicillin-resistant Staphylococcus aureus (MRSA) before, during and after bedmaking was investigated. Air was sampled with an Andersen air sampler in the rooms of 13 inpatients with MRSA infection or colonization. Sampling of surfaces, including floors and bedsheets, was performed by stamp methods. MRSA-containing particles were isolated on all the sampler stages-stage 1 (>7 microm diameter) to stage 6 (0.65-1.1 microm). The MRSA-containing particles were mostly 2-3 microm diameter before bedmaking and >5 microm during bedmaking. The number was significantly higher 15 min after bedmaking than during the resting period, but the differences in counts after 30 and 60 min were not significant. MRSA was detected on many surfaces. The results suggest that MRSA was recirculated in the air, especially after movement. To prevent airborne transmission, healthcare staff should exercise great care to disinfect inanimate environments. Further studies will be needed to confirm the level of MRSA contamination of air during bedmaking and establish measures for prevention of airborne transmission.
authors.affiliation: Department of Otorhinolaryngology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shiomori
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: K
authors.initials: K
authors.lastname: Makishima
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Fujiyoshi
authors.forename: T
authors.initials: T
authors.lastname: Udaka
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: N
authors.initials: N
authors.lastname: Hiraki
mesh_terms: D000391:Air Microbiology; D001512:Bedding and Linens; D003428:Cross Infection / Q000453:epidemiology; D004781:Environmental Exposure; D006797:Housekeeping, Hospital; D006801:Humans; D016106:Methicillin Resistance; D010362:Patients' Rooms; D012307:Risk Factors; D013203:Staphylococcal Infections / Q000453:epidemiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*