title: Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia.
abstract: OBJECTIVE
  Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis.
  
  
  METHODS
  Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. aureus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, alpha-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls.
  
  
  RESULTS
  Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid,alpha-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia.
authors.affiliation: Department of Infectious Diseases, Ostersund Hospital, Ostersund, Sweden. ulf.ryding@jll.se
authors.forename: U
authors.initials: U
authors.lastname: Ryding
authors.forename: F
authors.initials: F
authors.lastname: Espersen
authors.forename: B
authors.initials: B
authors.lastname: Söderquist
authors.forename: B
authors.initials: B
authors.lastname: Christensson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000097:blood; D016470:Bacteremia / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004697:Endocarditis, Bacterial / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008875:Middle Aged; D012189:Retrospective Studies; D012698:Serologic Tests; D013203:Staphylococcal Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000907:Antibodies, Bacterial