Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture.
Yarrowia lipolytica LGAM S(7)1 presented remarkable growth on industrial glycerol used as sole carbon substrate. Nitrogen-limited flask cultures were accompanied by restricted synthesis of reserve lipid, whilst amounts of citric acid were produced extracellularly. On the contrary, high amounts of reserve lipid (up to 3.5 g/l, 43% w/w of lipids in dry biomass) were produced in highly aerated continuous cultures. Lipid production was favoured at low specific dilution rates whilst fat-free material yield increased over the whole range of D (h(-1)). The maximum volumetric productivity obtained was 0.12 g lipid/1 h. Storage lipid composition did not present remarkable changes in the specific dilution rates tested. Oleate and linoleate were the dominant cellular fatty acids.
Papanikolaou
Seraphim
S
Laboratory of General and Agricultural Microbiology, Department of Agricultural Biotechnology, Agricultural University of Athens, Votanikos, Greece.
Aggelis
George
G
D018533:Biomass; D019343:Citric Acid / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D019787:Linoleic Acid / Q000096:biosynthesis / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D019301:Oleic Acid / Q000096:biosynthesis / Q000378:metabolism; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D008055:Lipids; D019343:Citric Acid; D019301:Oleic Acid; D019787:Linoleic Acid; D005990:Glycerol