title: Urinary concentration and tissue messenger RNA expression of monocyte chemoattractant protein-1 as an indicator of the degree of hydronephrotic atrophy in partial ureteral obstruction.
abstract: OBJECTIVE
  To find a potential prognostic marker of the induction of hydronephrotic atrophy in congenital hydronephrosis we investigated whether the messenger (m)RNA expression and urinary concentration of monocyte chemoattractant protein-1 (MCP-1) correlated with the degree of partial ureteral obstruction, and subsequent hydronephrotic atrophy and interstitial fibrosis.
  
  
  METHODS
  We created left partial ureteral obstruction in 96 juvenile Wistar rats and complete ureteral obstruction in 18, while 16 underwent sham operation. Depending on excretion of contrast medium into the renal pelvis after 3 days we defined 2 degrees of hydronephrosis. Renal mRNA expression of MCP-1, and renal pelvic and bladder urinary concentrations of MCP-1 were measured after 1, 2 and 3 weeks, and compared with the degree of hydronephrotic atrophy.
  
  
  RESULTS
  Grade 1 partial ureteral obstruction resulted in mild histological changes. Grade 2 partial and complete obstruction resulted in significant hydronephrotic atrophy. MCP-1 mRNA expression in the kidney remained unchanged in grade 1 partial obstruction but was moderately increased in grade 2 partial obstruction and clearly over expressed in complete ureteral obstruction. The renal pelvic urinary concentration of MCP-1 was not higher in rats with grade 1 partial obstruction than in sham operated animals but it was significantly increased in those with grade 2 partial and complete obstruction.
  
  
  CONCLUSIONS
  mRNA expression and the urinary concentration of MCP-1 correlate with the degree of obstruction and subsequent renal damage in hydronephrosis. They may serve as prognostic markers in children with congenital hydronephrosis.
authors.affiliation: Department of Urology, University of Hamburg, Eppendorf University Hospital, Hamburg, Germany.
authors.forename: Miriam
authors.initials: M
authors.lastname: Stephan
authors.forename: Stefan
authors.initials: S
authors.lastname: Conrad
authors.forename: Thilo
authors.initials: T
authors.lastname: Eggert
authors.forename: Roman
authors.initials: R
authors.lastname: Heuer
authors.forename: Salvador
authors.initials: S
authors.lastname: Fernandez
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huland
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D018932:Chemokine CCL2 / Q000378:metabolism* / Q000652:urine; D006869:Hydronephrosis / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D014517:Ureteral Obstruction / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2; D012333:RNA, Messenger