The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions.
In the present study, we show that the stress-inducible hsp70 gene in zebrafish is strongly and specifically expressed during normal lens formation from 28 to 38 hours post-fertilization, and is subsequently downregulated by 2 days of age. Only weak constitutive hsp70 mRNA signal was sporadically observed in other embryonic tissues. Similarly, transgenic fish carrying a 1.5 kb fragment of the hsp70 promoter linked to eGFP exhibited fluorescence only in the lens. In contrast, both the endogenous hsp70 gene and the transgene were strongly expressed throughout the embryo following heat shock at the same developmental stages.
Blechinger
Scott R
SR
Department of Anatomy and Cell Biology, Health Sciences Building, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan, Canada S7N 5E5.
Evans
Tyler G
TG
Tang
Ping Tao
PT
Kuwada
John Y
JY
Warren
James T
JT
Krone
Patrick H
PH
D000818:Animals; D030801:Animals, Genetically Modified; D015536:Down-Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics*; D006358:Hot Temperature; D017403:In Situ Hybridization; D007908:Lens, Crystalline / Q000196:embryology*; D008164:Luminescent Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D019076:Transgenes; D015027:Zebrafish
D018840:HSP70 Heat-Shock Proteins; D008164:Luminescent Proteins; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins