Barley viability during storage: use of magnetic resonance as a potential tool to study viability loss.
Malting-quality barley samples of the varieties Harrington, Manley, and TR118, each from two locations in Saskatchewan, were collected directly from the producers and sent to China for storage. At regular intervals samples were shipped back to Canada for analysis consisting of germination studies, alpha-amylase tests, magnetic resonance imaging (MRI), and magnetic relaxation (NMR) studies. Samples showing a decrease in germinative energy and elevated levels of alpha-amylase also showed a rapid uptake of water in the area between the embryo and the endosperm as observed by MRI. Using NMR relaxation experiments, viable and nonviable barley samples could be distinguished after 2 h of imbibition.
Gruwel
Marco L H
ML
National Research Council, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, SK, Canada. marco.gruwel@nrc.ca
Yin
Xiang S
XS
Edney
Michael J
MJ
Schroeder
Steve W
SW
MacGregor
Alex W
AW
Abrams
Suzanne
S
D005519:Food Preservation; D018525:Germination; D001467:Hordeum / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D012639:Seeds / Q000502:physiology; D014867:Water / Q000378:metabolism; D000516:alpha-Amylases / Q000032:analysis
D014867:Water; D000516:alpha-Amylases