title: The capsid of infectious bursal disease virus contains several small peptides arising from the maturation process of pVP2.
abstract: The capsid proteins VP2 and VP3 of infectious bursal disease virus, a birnavirus, are derived from the processing of a large polyprotein: NH2-pVP2-VP4-VP3-COOH. Although the primary cleavage sites at the pVP2-VP4 and VP4-VP3 junctions have been identified, the proteolytic cascade involved in the processing of this polyprotein is not yet fully understood, particularly the maturation of pVP2. By using different approaches, we showed that the processing of pVP2 (residues 1 to 512) generated VP2 and four small peptides (residues 442 to 487, 488 to 494, 495 to 501, and 502 to 512). We also showed that in addition to VP2, at least three of these peptides (residues 442 to 487, 488 to 494, and 502 to 512) were associated with the viral particles. The importance of the small peptides in the virus cycle was assessed by reverse genetics. Our results showed that the mutants lacking the two smaller peptides were viable, although the virus growth was affected. In contrast, deletions of the domain 442 to 487 or 502 to 512 did not allow virus recovery. Several amino acids of the peptide 502 to 512 appeared essential for virus viability. Substitutions of the P1 and/or P1" position were engineered at each of the cleavage sites (P1-P1": 441-442, 487-488, 494-495, 501-502, and 512-513). Most substitutions at the pVP2-VP4 junction (512-513) and at the final VP2 maturation cleavage site (441-442) were lethal. Mutations of intermediate cleavage sites (487-488, 494-495, and 501-502) led to viable viruses showing different but efficient pVP2 processing. Our data suggested that while peptides 488 to 494 and 495 to 501 play an accessory role, peptides 442 to 487 and 502 to 512 have an unknown but important function within the virus cycle.
authors.affiliation: Unité de Virologie et Immunologie Moléculaires. Unité de Biochimie et Structure des Protéines, Institut National de la Recherche Agronomique, F-78350 Jouy-en-Josas, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Da Costa
authors.forename: Christophe
authors.initials: C
authors.lastname: Chevalier
authors.forename: Celine
authors.initials: C
authors.lastname: Henry
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Huet
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Petit
authors.forename: Jean
authors.initials: J
authors.lastname: Lepault
authors.forename: Hein
authors.initials: H
authors.lastname: Boot
authors.forename: Bernard
authors.initials: B
authors.lastname: Delmas
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D007243:Infectious bursal disease virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism
chemical_list: D010455:Peptides; D011498:Protein Precursors; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins