Protein nanoarrays generated by dip-pen nanolithography.
Dip-pen nanolithography was used to construct arrays of proteins with 100- to 350-nanometer features. These nanoarrays exhibit almost no detectable nonspecific binding of proteins to their passivated portions even in complex mixtures of proteins, and therefore provide the opportunity to study a variety of surface-mediated biological recognition processes. For example, reactions involving the protein features and antigens in complex solutions can be screened easily by atomic force microscopy. As further proof-of-concept, these arrays were used to study cellular adhesion at the submicrometer scale.
Lee
Ki-Bum
KB
Northwestern University, Department of Chemistry and Center for Nanofabrication and Molecular Self-Assembly, 2145 Sheridan Road, Evanston, IL 60208, USA.
Park
So-Jung
SJ
Mirkin
Chad A
CA
Smith
Jennifer C
JC
Mrksich
Milan
M
D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D022001:Focal Adhesions; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D008904:Miniaturization; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology; D010169:Palmitic Acids / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D011940:Receptor Aggregation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
C450319:16-mercaptohexadecanoic acid; D005353:Fibronectins; D007074:Immunoglobulin G; D010169:Palmitic Acids; D011506:Proteins; D011994:Recombinant Proteins; D009113:Muramidase