title: Membrane reorganization during chilling: implications for long-term stabilization of platelets.
abstract: This essay is a review of the various biophysical and biochemical events that make up the factors responsible for platelet cold-induced activation. It describes the formation of large membrane domains composed of raft aggregates that occur during chilling and storage. It also presents strong evidence that platelet membranes undergo lateral phase separation during prolonged storage in the cold and suggests that raft aggregation and lateral phase separation are key events which must be obviated to stabilize platelets and store them either in the frozen or in the dry state.
authors.affiliation: Biostabilization Program, University of California, Davis, CA 95616, USA.
authors.forename: F
authors.initials: F
authors.lastname: Tablin
authors.forename: W F
authors.initials: WF
authors.lastname: Wolkers
authors.forename: N J
authors.initials: NJ
authors.lastname: Walker
authors.forename: A E
authors.initials: AE
authors.lastname: Oliver
authors.forename: N M
authors.initials: NM
authors.lastname: Tsvetkova
authors.forename: K
authors.initials: K
authors.lastname: Gousset
authors.forename: L M
authors.initials: LM
authors.lastname: Crowe
authors.forename: J H
authors.initials: JH
authors.lastname: Crowe
mesh_terms: D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D001793:Blood Preservation / Q000379:methods*; D002152:Calorimetry, Differential Scanning; D002462:Cell Membrane / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D004705:Endocytosis; D005612:Freeze Drying; D006801:Humans; D066298:In Vitro Techniques; D021962:Membrane Microdomains / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D015539:Platelet Activation / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D013997:Time Factors; D014199:Trehalose / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D014199:Trehalose