HPLC analysis of manno-oligosaccharides derived from Saccharomyces cerevisiae mannan using an amino column or a graphitized carbon column.
The chromatographic behavior of manno-oligosaccharides derived from Saccharomyces cerevisiae mannan on two kinds of HPLC columns, an aminopropyl-silica column or a graphitized carbon column (GCC), was investigated. The order of elution of manno-oligosaccharides on both columns with acetonitrile-water was almost the same, that is, the retention increased with increasing molecular size. However, the GCC made it possible to isolate completely two isomers of mannotrioses (M(3)-1 and M(3)-2) with different linkage positions. We reinvestigated the structures of mannobiose (M(2)), M(3)s, and mannotetraose (M(4)) that were completely isolated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and NMR spectroscopy.
Tanimoto
Toshiko
T
School of Pharmaceutical Sciences, Mukogawa Women s University, Nishinomiya, Japan. tanimoto@mwu.mukogawa-u.ac.jp
Ikuta
Akiko
A
Sugiyama
Mayumi
M
Koizumi
Kyoko
K
D002851:Chromatography, High Pressure Liquid; D006108:Graphite; D008351:Mannans / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D008351:Mannans; D009844:Oligosaccharides; C014647:mannobiose; C039922:mannotetraose; D006108:Graphite