Detection and quantification of Pfiesteria piscicida by using the mitochondrial cytochrome b gene.
Mitochondrial cytochrome b was isolated from the dinoflagellate Pfiesteria piscicida, and the utility of the gene for species identification was examined. One of the primer sets designed was shown to be highly specific for P. piscicida. A time step PCR protocol was used to demonstrate the potential of this primer set for quantification of this species.
Zhang
Huan
H
Department of Marine Sciences, University of Connecticut, Groton, CT 06340, USA.
Lin
Senjie
S
D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D020045:Pfiesteria piscicida / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014867:Water / Q000469:parasitology
D003573:Cytochrome b Group; D017931:DNA Primers; D012337:RNA, Ribosomal, 18S; D014867:Water