[Detection of telomerase activity in prostate needle-biopsy samples].
OBJECTIVE
To detect telomerase activity inprostate needle-biopsy samples and its role in diagnosis and prognosis of prostate cancer.


METHODS
20 tissue samples of prostate cancer tissues and 16 samples of adjacent tissues were obtained by needle-biopsy guided by transrectal B ultrasonography. 16 samples of benign prostatic hyperplasia tissues were obtained by prostatectomy and were confirmed pathologically. Telomerase activity was revealed by modified PCR-based telomeric repeat amplication protocol (TRAP)-silver staining assay.


RESULTS
Telomerase activity was revealed in 18 cases of prostate cancer. In tissues adjacent to prostate cancer, telomerase activity was found in 7 of 11 cases of prostatic intraepithelial neoplasia (PIN), and in 2 of 5 cases of BPH. None of tissue samples from patients with BPH showed telomerase activity.


CONCLUSIONS
Telomerase activity in prostate needle-biopsy samples might be an useful marker for detecting prostate cancer and biological malignancy.
Wang
X
X
Department of Urology, Huashan Hospital, Shanghai 200040.
Zhang
Y
Y
Ding
Q
Q
Ye
L
L
Zhai
L
L
Jia
W
W
D000230:Adenocarcinoma / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000097:blood*; D001707:Biopsy, Needle; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood; D011470:Prostatic Hyperplasia / Q000201:enzymology / Q000473:pathology; D019048:Prostatic Intraepithelial Neoplasia / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000473:pathology; D019098:Telomerase / Q000378:metabolism*
D014408:Biomarkers, Tumor; D019098:Telomerase; D017430:Prostate-Specific Antigen