title: [IL-12 gene treatment of hepatocellular carcinoma: experimental study].
abstract: OBJECTIVE
  To investigate the inhibitory effects of retrovirus vector containing IL-12 gene on hepatoma growth in vivo and to explore a new approach of gene therapy to hepatocellular carcinoma (HCC).
  
  
  METHODS
  Retrovirus vector containing IL-12 gene was constructed and transfected into packaged cell PA317. Positive PA317 was injected into the rat which suffered from experimental HCC and its anti-tumor effects and immunity changes were recorded.
  
  
  RESULTS
  The packaged cell PA317 containing IL-12 gene could inhibit the proliferation of hepatocellular cell line CBRH3. The rats injected at day 1 or 3 can survive permanently, while those injected at day 5 or 7 can survive longer than those not injected. However, tumor generated in rats injected with blank control or package cells containing retroviral vector without IL-12 gene (P < 0.01).
  
  
  CONCLUSIONS
  Package cells transfected with retroviral vector containing IL-12 either injected to the hepatoma tissue locally or given splenic exercises anti-hepatoma effects efficiently. The direct intrasplenic injection route is new, safe and effective.
authors.affiliation: Tumor Immunology and Gene Therapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai 200438.
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: Q
authors.initials: Q
authors.lastname: Qian
authors.forename: H
authors.initials: H
authors.lastname: Xue
authors.forename: H
authors.initials: H
authors.lastname: Cao
authors.forename: Z
authors.initials: Z
authors.lastname: Cui
authors.forename: W
authors.initials: W
authors.lastname: Shi
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors; D015552:Injections, Intralesional; D018664:Interleukin-12 / Q000235:genetics* / Q000627:therapeutic use; D008114:Liver Neoplasms, Experimental / Q000628:therapy*; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D012190:Retroviridae / Q000235:genetics; D014162:Transfection / Q000379:methods
chemical_list: D018664:Interleukin-12