[Correlation between gene expression of CCK-A receptor and gallbladder emptying in gallstone patients].
OBJECTIVE
To investigate the expression of CCK-A receptor and its relation with gallbladder hypomotility in patients with gallstone.


METHODS
20 patients with gallstone and 10 normal subjects were studied and gallbladder emptying function was measured by B ultrasonography. The other 8 patients without gallstone who died of accident were selected as controls for measuring mRNA expression of CCK-A receptor of gallbladder; RT-PCR was used to explore gene expression of CCK-A receptor of gallbladder.


RESULTS
The gallbladder motility was significantly impaired in gallstone patients. The patients had lower mRNA expression of CCK-A receptor than control subjects (0.633 +/- 0.167 vs. 0.944 +/- 0.230, P < 0.01), and so did patients with impaired gallbladder motility then those with normal motility (0.544 +/- 0.124 vs. 0.768 +/- 0.131, P < 0.05). Furthermore, the change of expression of CCK-A receptors was markedly correlated with gallbladder emptying (r = 0.925, P < 0.01).


CONCLUSIONS
mRNA expression of CCK-A receptor in gallstone patients decreases, which was related to gallbladder hypomotility. The down-regulation of gene expression of CCK-A receptor plays an important role in gallbladder hypomotility in patients with gallstone.
Shuai
J
J
Department of Surgery, Ruijing Hospital, Shanghai Second Medical University, Shanghai 200025.
Zhang
S
S
Han
T
T
Jiang
Y
Y
Lei
R
R
Chen
S
S
D041761:Cholecystolithiasis / Q000235:genetics / Q000503:physiopathology*; D016473:Gallbladder Emptying; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D043886:Receptor, Cholecystokinin A / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D043886:Receptor, Cholecystokinin A