A DMSO-sensitive conditional mutant of the fission yeast orthologue of the Saccharomyces cerevisiae SEC13 gene is defective in septation.
Dissection of complex processes using model organisms such as yeasts relies heavily upon the use of conditional mutants. We have generated a collection of fission yeast mutants sensitive to dimethylsulphoxide (DMSO). Among these we have found a mutant in the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae SEC13 gene, which fails to cleave the division septum. Generation of a null allele demonstrates that the S. pombe sec13 gene is essential.
Poloni
Deborah
D
Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), 1066 Epalinges, Switzerland.
Simanis
Viesturs
V
D000595:Amino Acid Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D003001:Cloning, Molecular; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D020043:Genes, Essential / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D009154:Mutation / Q000187:drug effects / Q000235:genetics*; D028861:Nuclear Pore Complex Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; C079620:SEC13 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D004121:Dimethyl Sulfoxide