Targeting of liposomes to human keratinocytes through adhesive peptides from immunoglobulin domains in the presence of IFN-gamma.
T-cell adhesion is often dictated by the presence of intercellular adhesion molecule-1 (ICAM-1) on the target cell surface. Reconstitution of P(0) protein into liposomes increases adhesion to melanoma cells expressing ICAM-1. In our study, the effect of peptides derived from P(0) protein and leukocyte function associated-antigen 1 (LFA-1) on IFN-gamma-stimulated human keratinocytes was investigated. Covalently linked P(0)-peptide-1, from the Ig-like domain, increased specific liposome binding to IFN-gamma-stimulated keratinocytes in a dose-dependent manner. C-terminal-derived P(0)-peptide-3 increased liposome binding nonspecifically. LFA-1 and RGD peptides had no apparent effect. P(0)-peptide-1 is thus a potential targeting ligand for liposomal drug delivery to ICAM-1 expressing keratinocytes in inflammatory dermatoses.
Jaafari
M R
MR
College of Pharmacy & Nutrition, University of Saskatchewan, Saskatoon, SK, Canada.
Foldvari
M
M
D002448:Cell Adhesion; D004337:Drug Carriers; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D019169:Jurkat Cells; D015603:Keratinocytes / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism* / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D018993:Myelin P0 Protein / Q000378:metabolism; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D011994:Recombinant Proteins; D012867:Skin / Q000166:cytology
D004337:Drug Carriers; D008081:Liposomes; D016169:Lymphocyte Function-Associated Antigen-1; D018993:Myelin P0 Protein; D010455:Peptides; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma