title: Decreased expression of L-selectin on peripheral blood polymorphonuclear leukocytes in neonates with severe asphyxia.
abstract: The expression of adherent leukocyte surface molecules, L-selectin (CD62L), and Mac-1 (CD11b/CD18) in peripheral blood polymorphonuclear leukocytes (PMNs) was studied in 48 neonates and 24 healthy adults using fluorescence flow cytometry. L-selectin expression was decreased significantly in neonates, especially those with severe asphyxia (n = 10) compared with adults. Mac-1 expression was significantly increased in neonates compared with adults, but did not differ between 24 normal neonates, 14 neonates with mild asphyxia, and 10 neonates with severe asphyxia. These results suggest that the magnitude of the decrease in L-selectin expression reflects the severity of asphyxia in neonates.
authors.affiliation: Neonatal Unit, Children's Hospital Hachioji, Hachioji, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Nishida
authors.forename: Hisanori
authors.initials: H
authors.lastname: Minakami
authors.forename: Yukio
authors.initials: Y
authors.lastname: Takashima
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kato
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Okada
authors.forename: Yunosuke
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Susumu
authors.initials: S
authors.lastname: Itoh
authors.forename: Hiroazu
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000328:Adult; D001238:Asphyxia Neonatorum / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D019041:L-Selectin / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: D016177:Macrophage-1 Antigen; D019041:L-Selectin