ELISA assays for the detection of Bothrops lanceolatus venom in envenomed patient plasmas.
A double antibody sandwich enzyme linked immunosorbant assay (ELISA) was carried out to detect Bothrops Ianceolatus venom in plasma from envenomed patients at various time intervals (0, 6, 12, 18 and 24 hrs). The test could detect Bothrops lanceolatus levels up to 12 ng/mL of envenomed patient plasmas. Elaboration of an easy, fast and species-diagnostic based on this ELISA technique useful to physicians is discussed.
Rodriguez-Acosta
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A
Universidad Central de Venezuela, Seccion de Immunoquimica, Instituto de Medicina Tropical, Venezuela. rodriguf@camelot.rect.ucv.ve
Uzcategui
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W
Azuaje
R
R
Giron
M E
ME
Aguilar
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I
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D012909:Snake Bites / Q000097:blood* / Q000175:diagnosis; D013045:Species Specificity; D013997:Time Factors
D003435:Crotalid Venoms