Simultaneous measurement of zolmitriptan and its major metabolites N-desmethylzolmitriptan and zolmitriptan N-oxide in human plasma by high-performance liquid chromatography with coulometric detection.
Zolmitriptan, N-desmethylzolmitriptan, zolmitriptan N-oxide and an internal standard (an analogue of zolmitriptan) were extracted from plasma by a solid-phase extraction (SPE). Chromatography was performed using isocratic reversed-phase high-performance liquid chromatography (HPLC) with coulometric end-point detection. The standard curves were linear over the range 2-20 ng/ml for zolmitriptan and its metabolites in plasma. The mean inter- and intra-assay coefficients of variation over the range of the standard curves were less than 11%. The absolute recovery averaged 87, 58 and 77% for zolmitriptan. N-desmethylzolmitriptan and zolmitriptan N-oxide, respectively. The assay sensitivity was 0.5 ng for each analyte.
Clement
E M
EM
University of Oxford, Department of Psychiatry, Warneford Hospital, UK.
Franklin
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D023303:Oxazolidinones / Q000097:blood* / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014363:Tryptamines
D023303:Oxazolidinones; D014363:Tryptamines; C089750:zolmitriptan