Ion/ion chemistry as a top-down approach for protein analysis.
Developing methodology for analyzing complex protein mixtures in a rapid fashion is one of the most challenging problems facing analytical biochemists today. Recent advances in mass spectrometry for the analysis of intact proteins (i.e. the top-down approach) show great promise for rapid protein identification. The ion/ion chemistry approach for the detection and identification of target proteins in complex matrices, determination of fragmentation channels as a function of precursor ion charge state, and post-translational modification characterization are discussed with particular emphasis on tandem mass spectrometry of intact proteins.
Stephenson
James L
JL
Research Triangle Institute, 3040 Cornwallis Road, PO Box 12194, Research Triangle Park, NC 27709-2194, USA. stephensonjl@rti.org
McLuckey
Scott A
SA
Reid
Gavin E
GE
Wells
J Mitchell
JM
Bundy
Jonathan L
JL
D007477:Ions / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
D007477:Ions; D011506:Proteins