Electrophoretic behavior of adamantane derivatives possessing antiviral activity and their determination by capillary zone electrophoresis with indirect detection.
Separation and determination of adamantane derivatives with antiviral activity, namely amantadine (1-adamantan amine), memantine (1-amino-3,5-dimethyl adamantane) and rimantadine (alpha-methyl-1-adamantane methylamine), were examined by capillary zone electrophoresis. After optimization, an indirect detection method using 5 mM 4-methylbenzylamine in ethanol/water solution (1:4) as simultaneously absorbing and buffering background electrolyte with detection at 210 nm was found suitable for determination of the individual compounds (limit of detection was 0.35 mg L(-1) for memantine hydrochloride, S/N = 3). Baseline separation of all the three compounds was reached by addition of alpha- or beta-cyclodextrins to the electrolyte in concentrations of 20 and 2 mM, respectively.
Reichová
Nad'a
N
Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Pazourek
Jirí
J
Polásková
Pavla
P
Havel
Josef
J
D000547:Amantadine / Q000032:analysis*; D000998:Antiviral Agents / Q000032:analysis*; D003505:Cyclodextrins; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D008559:Memantine / Q000032:analysis*; D012299:Rimantadine / Q000032:analysis*; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins
D000998:Antiviral Agents; D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D012299:Rimantadine; D000547:Amantadine; C031215:betadex; D008559:Memantine; C032613:alpha-cyclodextrin