title: [A novel immunization schedule for obtaining anti-LPS Haemophilus influenzae type b antibodies].
abstract: A novel immunization schedule was tested to increase antibodies to Haemophilus influenzae type B in which a 0.5% formaldehyde-inactivated cellular antigen and a partially purified lipopolysacharid antigen obtained from a selective precipitation with organic solvents were combined as immunogens. High specificity and biological activity antibodies with titers up to 1:256 were obtained by counterinmunoelectrophoresis; they were purified through a selective precipitation with ammonium sulphate and an ion-exchange chromatography using a DEAE-Sapharose column. The method proposed in this paper facilitates the production of purified antibodies useful for the improvement of serological diagnosis and H. influenzae b strain serotyping techniques.
authors.affiliation: ciipk@ipk.sld.cu
authors.forename: R
authors.initials: R
authors.lastname: Falcón Márquez
authors.forename: G
authors.initials: G
authors.lastname: Toraño Peraza
authors.forename: B
authors.initials: B
authors.lastname: Victoria Martínez
authors.forename: I
authors.initials: I
authors.lastname: Tamargo Martínez
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D019741:Haemophilus influenzae type b / Q000276:immunology*; D007115:Immunization Schedule; D008070:Lipopolysaccharides / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides