Rhythmic expression of ROR beta mRNA in the mice suprachiasmatic nucleus.
The expression of the brain rich orphan nuclear receptor ROR beta (retinoid-related orphan receptor beta) was investigated in the mouse brain by in situ hybridization using antisense cRNA probe. Positive ROR beta mRNA signals were detected in various parts of the brain with high expression in the suprachiasmatic nucleus (SCN). In the SCN, ROR beta mRNA signals showed a peak at early daytime (ZT/CT4) and a trough at early nighttime (ZT/CT16) in both light-dark and constant dark conditions. Light exposure at subjective night did not alter the expression level. These findings suggest that ROR beta is a new member of a transcription factor possibly related to the circadian pacemaking system.
Sumi
Yasuo
Y
Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Yagita
Kazuhiro
K
Yamaguchi
Shun
S
Ishida
Yoshiki
Y
Kuroda
Yoshikazu
Y
Okamura
Hitoshi
H
D000818:Animals; D001683:Biological Clocks / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000378:metabolism; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000502:physiology*; D015536:Down-Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000033:anatomy & histology / Q000378:metabolism*; D009043:Motor Activity / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism; D057095:Nuclear Receptor Subfamily 1, Group F, Member 2; D056950:Period Circadian Proteins; D010775:Photic Stimulation; D012333:RNA, Messenger / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics*; D018160:Receptors, Cytoplasmic and Nuclear; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors; D015854:Up-Regulation / Q000502:physiology
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D057095:Nuclear Receptor Subfamily 1, Group F, Member 2; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors