title: Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.).
abstract: Three ethylene receptor genes, DC-ERS1, DC-ERS2 and DC-ETR1, were previously identified in carnation (Dianthus caryophyllus L.). Here, the presence of mRNAs for respective genes in flower tissues and their changes during flower senescence are investigated by Northern blot analysis. DC-ERS2 and DC-ETR1 mRNAs were present in considerable amounts in petals, ovaries and styles of the flower at the full-opening stage. In the petals the level of DC-ERS2 mRNA showed a decreasing trend toward the late stage of flower senescence, whereas it increased slightly in ovaries and was unchanged in styles throughout the senescence period. However, DC-ETR1 mRNA showed no or little changes in any of the tissues during senescence. Exogenously applied ethylene did not affect the levels of DC-ERS2 and DC-ETR1 mRNAs in petals. Ethylene production in the flowers was blocked by treatment with 1,1-dimethyl-4-(phenylsulphonyl)semicarbazide (DPSS), but the mRNA levels for DC-ERS2 and DC-ETR1 decreased in the petals. DC-ERS1 mRNA was not detected in any cases. These results indicate that DC-ERS2 and DC-ETR1 are ethylene receptor genes responsible for ethylene perception and that their expression is regulated in a tissue-specific manner and independently of ethylene in carnation flowers during senescence.
authors.affiliation: Laboratory of Bio-adaptation, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shibuya
authors.forename: Masayasu
authors.initials: M
authors.lastname: Nagata
authors.forename: Natsu
authors.initials: N
authors.lastname: Tanikawa
authors.forename: Toshihito
authors.initials: T
authors.lastname: Yoshioka
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Hashiba
authors.forename: Shigeru
authors.initials: S
authors.lastname: Satoh
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D029749:Dianthus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005030:Ethylenes / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018514:Plant Structures / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D012663:Semicarbazides / Q000494:pharmacology
chemical_list: C114574:ERS2 protein, Dianthus; D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D012663:Semicarbazides; C083750:ethylene receptors, plant; C036216:ethylene