[Molecular cloning of specific porcine adipocyte membrane protein cDNA].
Specific porcine adipocyte membrane protein (PAMP), which was recently reported, is probably associated with the growth and development of adipocyte and regulator of adipose tissue. Using RT-PCR and RACE methods, and primer designed according to published sequence of PAMP gene 5' end, we have successfully amplified about a 500 bp and a 400 bp from pig adipose tissue. A 500 bp fragment was retrieved, cloned and sequenced. The sequencing result showed that we have got the clone and sequence of cDNA 5'-end for PAMP gene.
Lin
Ju-Qiang
JQ
College of Animal Husbandry and Veterinary, Huazhong Agricultural University, Wuhan 430070, China.
Jiang
Si-Wen
SW
Zheng
Rong
R
Peng
Jian
J
Xiong
Yuan-Zhu
YZ
D017667:Adipocytes / Q000502:physiology*; D000273:Adipose Tissue / Q000254:growth & development*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
D018076:DNA, Complementary; D008565:Membrane Proteins