Simple method for reduction of autofluorescence in fluorescence microscopy.
Autofluorescence of aldehyde-fixed neural tissue often complicates the use of fluorescence microscopy. Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes, resulting in a higher contrast without adversely affecting the staining probabilities.
Neumann
Michael
M
Department of Chemistry, University of Bremen, Bremen, Germany.
Gabel
Detlef
D
D000818:Animals; D001932:Brain Neoplasms / Q000737:chemistry; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods* / Q000592:standards*; D011786:Quality Control; D016610:Tissue Embedding; D014466:Ultraviolet Rays
D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein