title: Identification of a novel WT1-derived peptide which induces human leucocyte antigen-A24-restricted anti-leukaemia cytotoxic T lymphocytes.
abstract: We previously reported the establishment of a Wilms' tumour (WT)1-derived peptide (CMTWNQMNL)-specific and human leucocyte antigen (HLA)-A24-restricted anti-leukaemia cytotoxic T lymphocyte (CTL) line, TAK-1. In this study, we have established a novel WT1-derived peptide (RWPSCQKKF)-specific CD8+ CTL line, designated NIM-1. NIM-1 lysed HLA-A24-positive leukaemia cells, but not HLA-A24-negative leukaemia cells or normal cells. The effects of TAK-1 and NIM-1 on cytotoxicity against leukaemia cells were not synergistic, suggesting that recognition of a single epitope on the tumour-specific antigen by CTLs is sufficient to exert maximal cytotoxic activity against tumour cells.
authors.affiliation: First Department of Internal Medicine, Ehime University School of Medicine, Shinegobu, Ehime, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Azuma
authors.forename: Masanori
authors.initials: M
authors.lastname: Makita
authors.forename: Kanji
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Shigeru
authors.initials: S
authors.lastname: Fujita
authors.forename: Mine
authors.initials: M
authors.lastname: Harada
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasukawa
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D015234:HLA-A Antigens / Q000276:immunology*; D059871:HLA-A24 Antigen; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D014407:Tumor Cells, Cultured; D025721:WT1 Proteins / Q000276:immunology*
chemical_list: D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D025721:WT1 Proteins