Whole genome amplification--applications and advances.
The concept of whole genome amplification is something that has arisen in the past few years as the polymerase chain reaction (PCR) has been adapted to replicate regions of genomes that are of biological interest. The applications are many--forensic science, embryonic disease diagnosis, bioterrorism genome detection, "immortalization" of clinical samples, microbial diversity, and genotyping. Several recent papers suggest that whole genomes can be replicated without bias or non-random distribution of the target, these findings open up a new avenue to molecular biology.
Hawkins
Trevor L
TL
US DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA. tlhawkins@lbl.gov
Detter
John C
JC
Richardson
Paul M
PM
D000818:Animals; D016678:Genome; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000639:trends*; D016133:Polymerase Chain Reaction / Q000639:trends*