Induction of apoptosis in HL-60 cells by photochemically generated hydroxyl radicals.
Reactive oxygen species (ROS), especially hydroxyl radicals are postulated to mediate apoptosis of the cell. Here we demonstrate that hydroxyl radicals generated selectively by photolysis of a photo-Fenton reagent, N,N'-bis(2-hydroperoxy-2-methoxyethyl)-1,4,5,8-naphthaldiimide (NP-III), induce apoptosis in HL-60 (human promyelocytic leukemia) cells involving the activation of caspase-3.
Haruna
Sakiko
S
Department of Food Science and Nutrition, Nara Women's University, 630-8506, Nara, Japan.
Kuroi
Rie
R
Kajiwara
Kazumi
K
Hashimoto
Ryoko
R
Matsugo
Seiichi
S
Tokumaru
Sadako
S
Kojo
Shosuke
S
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D017665:Hydroxyl Radical / Q000494:pharmacology* / Q000528:radiation effects; D009281:Naphthalenes; D010777:Photochemistry; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species / Q000494:pharmacology / Q000528:radiation effects; D014466:Ultraviolet Rays
D009281:Naphthalenes; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; C067580:N,N'-bis(2-hydroxyperoxy-2-methoxyethyl)-1,4,5,8-naphthalenetetracarboxylic diimide; D017665:Hydroxyl Radical; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases