Identification of an apical Cl(-)/HCO3(-) exchanger in the small intestine.
HCO3(-) secretion is the most important defense mechanism against acid injury in the duodenum. However, the identity of the transporter(s) mediating apical HCO3(-) secretion in the duodenum remains unknown. A family of anion exchangers, which include downregulated in adenoma (DRA or SLC26A3), pendrin (PDS or SLC26A4), and the putative anion transporter (PAT1 or SLC26A6) has recently been identified. DRA and pendrin mediate Cl(-)/base exchange; however, the functional identity and distribution of PAT1 (SLC26A6) is not known. In these studies, we investigated the functional identity, tissue distribution, and membrane localization of PAT1. Expression studies in Xenopus oocytes demonstrated that PAT1 functions in Cl(-)/HCO3(-) exchange mode. Tissue distribution studies indicated that the expression of PAT1 is highly abundant in the small intestine but is low in the colon, a pattern opposite that of DRA. PAT1 was also abundantly detected in stomach and heart. Immunoblot analysis studies identified PAT1 as a approximately 90 kDa protein in the duodenum. Immunohistochemical studies localized PAT1 to the brush border membranes of the villus cells of the duodenum. We propose that PAT1 is an apical Cl(-)/HCO3(-) exchanger in the small intestine.
Wang
Zhaohui
Z
Department of Medicine, University of Cincinnati, Ohio 45267-0585, USA.
Petrovic
Snezana
S
Mann
Elizabeth
E
Soleimani
Manoocher
M
D000595:Amino Acid Sequence; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D002462:Cell Membrane / Q000737:chemistry; D027963:Chloride-Bicarbonate Antiporters / Q000032:analysis*; D003106:Colon / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000737:chemistry* / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D000076988:Sulfate Transporters; D014018:Tissue Distribution; D014162:Transfection; D014981:Xenopus
D001639:Bicarbonates; D002352:Carrier Proteins; D027963:Chloride-Bicarbonate Antiporters; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C439529:SLC26A6 protein, human; D000076988:Sulfate Transporters