title: The integrated genome map of Mycobacterium leprae.
abstract: The integrated map of the Mycobacterium leprae genome unveiled for the first time the genomic organization of this obligate intracellular parasite. Selected cosmid clones, isolated from a genomic library created in the cosmid vector Lorist6, were identified as representing nearly the complete genome and were subsequently used in the M. leprae genome sequencing project. Now a new version of the integrated map of M. leprae can be presented, combining the mapping results from the Lorist6 cosmids with data obtained from a second genomic library constructed in an Escherichia coli-mycobacterium shuttle cosmid, pYUB18. More than 98% of the M. leprae genome is now covered by overlapping large insert genomic clones representing a renewable source of well defined DNA segments and a powerful tool for functional genomics.
authors.affiliation: Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28, rue du Dr Roux, 75724 Paris, France. kei@pasteur.fr
authors.forename: K
authors.initials: K
authors.lastname: Eiglmeier
authors.forename: S
authors.initials: S
authors.lastname: Simon
authors.forename: T
authors.initials: T
authors.lastname: Garnier
authors.forename: S T
authors.initials: ST
authors.lastname: Cole
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016680:Genome, Bacterial; D006801:Humans; D007918:Leprosy / Q000382:microbiology*; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics*