EGFR and p53 expression and proliferative activity in parathyroid adenomas; an immunohistochemical study.
EGFR (epidermal growth factor receptor), p53, and proliferative markers provide some clues as to the formation of several tumours. In this study the mechanism of the genesis of parathyroid adenomas was investigated using immunohistochemistry. Sections of parathyroid adenomas from 12 cases were stained using PCNA (proliferating cell nuclear antigen), EGFR, and p53 immunohistochemistry. Correlations between PCNA LI (labelling index), EGFR expression, p53 expression, age, serum parathormone, Ca and P levels, and tumour diameter were investigated. PCNA LI was 45.8+/-33.1 (mean+/-standard deviation) and all the cases were somewhat positive. Five cases (41.67 %) were EGFR positive. Maximum 10 % of the cells were positive in these cases. All the cases were p53 negative. There was a correlation between PCNA LI and serum parathormone level (r=0.607, p=0.036). According to these results, parathormone synthesis is high when the proliferative activity of parathyroid adenoma is high. Four of the five EGFR-positive patients were below 35 years of age. These data may indicate that formation of parathyroid adenoma in young patients is related to a mechanism involving EGFR. Absence of p53 expression suggests that p53 mutation is not a common component of parathyroid adenomas.
Gülkesen
K H
KH
Akdeniz University, Pathology Department, Antalya, Turkey. gulkesen@med.akdeniz.edu.tr
Kiliçarslan
B
B
Altunbaş
H A
HA
Karpuzoglu
G
G
D000236:Adenoma / Q000737:chemistry* / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division; D066246:ErbB Receptors / Q000032:analysis*; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009154:Mutation; D010281:Parathyroid Hormone / Q000097:blood; D010282:Parathyroid Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D010281:Parathyroid Hormone; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D066246:ErbB Receptors