[TNF-alpha, IL-8 and IL-6 in the early inflammatory stage of experimental PVR model induced by macrophages].
OBJECTIVE
To observe the levels of cytokines, tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8) and IL-6 in the early inflammatory stages of a rabbit proliferative vitreoretinopathy (PVR) model induced by macrophages.


METHODS
The vitreous and venous blood of rabbit eyes of the PVR model were obtained and tested with ELISA kits of TNF-alpha, IL-8 and IL-6 and analyzed statistically.


RESULTS
The TNF-alpha level increased at the 7th day after macrophage injection and reached its peak (309 pg/ml) on day 21. IL-8 and IL-6 maintained their higher levels during 7 to 21 days after macrophage injection and the peak levels were 1 325 pg/ml and 998 pg/ml on day 14 respectively. Those showed significant differences compared to control (P <or= 0.05).


CONCLUSIONS
The 3 cytokines, TNF-alpha, IL-8 and IL-6, present high levels in the early stage and proliferative phase of the PVR model and decrease to normal level in the regeneration (scarring) phase, indicating that these cytokines may play an important role in initiating and modulating the cellular proliferation.
Hui
Y
Y
Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032.
Shi
Y
Y
Zhang
X
X
Yang
K
K
Yu
C
C
D000818:Animals; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008264:Macrophages; D008297:Male; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism* / Q000473:pathology
D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha