title: Alicyclobacillus herbarius sp. nov., a novel bacterium containing omega-cycloheptane fatty acids, isolated from herbal tea.
abstract: A thermo-acidophilic gram-positive bacterium, strain CP-1T, which grows aerobically at 35-65 degrees C (optimum 55-60 degrees C) and at pH 3.5-6.0 (optimum pH 4.5-5.0), was isolated from a herbal tea made from the dried flowers of hibiscus. Phylogenetic analysis of the 16S rRNA gene sequences showed that this bacterium was clearly distinguishable from previously described species of the genera Alicyclobacillus and Sulfobacillus. Strain CP-1T had unique omega-cycloheptane fatty acids as the major membrane lipid component, a characteristic which is peculiar to Alicyclobacillus cycloheptanicus. However, phenotypic and chemotaxonomic characteristics of strain CP-1T were different from those of the type strain of A. cycloheptanicus. DNA-DNA hybridization between the type strains of Alicyclobacillus species and Sulfobacillus disulfidooxidans was <20%, indicating that strain CP-1T represents a distinct species. On the basis of these results, the name Alicyclobacillus herbarius is proposed for this organism. The type strain is strain CP-1T (= DSM 13609T = IAM 14883T = NRIC 0477T).
authors.affiliation: Food Research Laboratories, Mitsui Norin Co. Ltd, Fujieda, Shizuoka, Japan. kgoto@mnk.co.jp
authors.forename: Keiichi
authors.initials: K
authors.lastname: Goto
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Matsubara
authors.forename: Kaoru
authors.initials: K
authors.lastname: Mochida
authors.forename: Terumi
authors.initials: T
authors.lastname: Matsumura
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Hara
authors.forename: Motohiro
authors.initials: M
authors.lastname: Niwa
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Yamasato
mesh_terms: D001628:Beverages / Q000382:microbiology*; D003508:Cycloheptanes / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D016937:Gram-Positive Endospore-Forming Rods / Q000737:chemistry* / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019660:Malvaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018514:Plant Structures / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D003508:Cycloheptanes; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S