title: Release of TNF-alpha from lipopolysaccharide (LPS)-stimulated Kupffer cells in serum- and nutrient-free medium.
abstract: In monocytes/macrophages LPS stimulation occurs by the binding of LPS and the serum component LPS-binding protein (LBP) to CD14. This study was conducted to investigate whether this mechanism also occurs in Kupffer cells. Rat Kupffer cells were stimulated for up to 8 h by LPS (0, 100 ng/ml, 10 microg/ml) in RPMI medium or in nutrient-free Krebs-Henseleit (KH) buffer. Some incubations were performed without serum, while in others serum was provided. TNF-alpha concentrations of the supernatants were measured by ELISA. LPS stimulation of Kupffer cells yielded the following results. In KH without any additives a considerable amount of TNF-alpha was released. Incubation in RPMI without serum caused twice as much TNF-alpha to be released as when KH was used. The addition of autologous serum to RPMI did not increase TNF-alpha response. These results provide evidence that a substantial part of TNF-alpha release by LPS-stimulated Kupffer cells occurs in a serum- and thus LBP-independent way.
authors.affiliation: University of Tübingen, Department of General Surgery, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Zipfel
authors.forename: M
authors.initials: M
authors.lastname: Schenk
authors.forename: B
authors.initials: B
authors.lastname: Metzdorf
authors.forename: C
authors.initials: C
authors.lastname: Bode
authors.forename: R
authors.initials: R
authors.lastname: Viebahn
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D012709:Serum Albumin / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D008070:Lipopolysaccharides; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D012710:Serum Albumin, Bovine