Identification of RNA species in the RNA-toxin complex and structure of the complex in Clostridium botulinum type E.
Clostridium botulinum type E toxin was isolated in the form of a complex with RNA(s) from bacterial cells. Characterization of the complexed RNA remains to be elucidated. The RNA is identified here as ribosomal RNA (rRNA) having 23S and 16S components. The RNA-toxin complexes were found to be made up of three types with different molecular sizes. The three types of RNA-toxin complex are toxin bound to both the 23S and 16S rRNA, toxin bound to the 16S rRNA and a small amount of 23S rRNA, and toxin bound only to the 16S rRNA.
Kitamura
Masaru
M
Laboratory of Bacterial Toxins, Department of Bacteriology, National Institute of Infectious Diseases (formerly National Institute of Health), 1-23-1, Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan. kmasaru@nih.go.jp
D001905:Botulinum Toxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D003014:Clostridium botulinum / Q000737:chemistry* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005779:Immunodiffusion; D046911:Macromolecular Substances; D008970:Molecular Weight; D012329:RNA, Bacterial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D001905:Botulinum Toxins