title: Determination of amitriptyline in plasma samples by high-performance liquid chromatography.
abstract: A simple and selective high-performance liquid chromatographic method for the determination of amitriptyline in human plasma has been developed. For plasma samples, the protein was removed with 1 M NaOH and 0.7 M ZnSO4. aqueous solutions. The chromatographic separation was performed on an analytical mbondapak C18 column (250 3.9 mm, i.d) with an isocratic mobile phase consisting of phosphate buffer-acetonitrile-triethylamine (65:35:0.1 v/v/v) adjusted to pH 5.1. Clomipramine was used as an internal standard. Using ultraviolet detection at 239 nm, the detection limit for amitriptyline in plasma was 5 ng/ml. No interferences were found with tricyclic antidepressant drugs, which allows this method to be used in clinical studies. The calibration curve was linear over the concentration range 5-200 ng/ml. The recovery was complete for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 10%.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Shaheed Beheshti, University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zarghi
authors.forename: S
authors.initials: S
authors.lastname: Dadashzadeh
authors.forename: Z
authors.initials: Z
authors.lastname: Kiayi
mesh_terms: D000639:Amitriptyline / Q000097:blood*; D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000929:Antidepressive Agents, Tricyclic; D012996:Solutions; D000639:Amitriptyline