Participation of two N-terminal residues in LPS-neutralizing activity of sarcotoxin IA.
Sarcotoxin IA is a cecropin-type antibacterial peptide of flesh fly. Using a mutant sarcotoxin IA lacking two N-terminal residues, we demonstrated that these residues are indispensable for its antibacterial activity against Escherichia coli and LPS-binding. Contrary to the native sarcotoxin IA, the mutant sarcotoxin IA could not neutralize various biological activities of LPS. It was suggested that sarcotoxin IA firmly binds to the lipid A core of LPS via these two N-terminal residues and forms a stable binding complex that exhibits no appreciable biological activity like native LPS.
Okemoto
Kazuo
K
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
Nakajima
Yuki
Y
Fujioka
Toshiyuki
T
Natori
Shunji
S
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D015152:Blotting, Northern; D002478:Cells, Cultured; D004175:Diptera; D005947:Glucose / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009500:Neutralization Tests; D010743:Phospholipids / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000596:Amino Acids; D000890:Anti-Infective Agents; D019476:Insect Proteins; D008070:Lipopolysaccharides; D008081:Liposomes; D010743:Phospholipids; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; C054733:sarcotoxin IA protein, Sarcophaga peregrina; D005947:Glucose