Emerging array-based technologies in proteomics.
Microarray format is the method of choice for high-throughput hybridization of nucleic acids. Lately, microarray strategy has been applied to broad studies of interactions of proteins with other molecules. The diversity of these interactions and variations of the properties of proteins present new challenges to microchip technology. Significant achievements have been reported. In particular, three-dimensional (gel-based) chips can be manufactured with limited resources and offer flexibility, capacity, homogeneous solvent environment and the ability to record processes in real time and in variable conditions.
Mirzabekov
Andrei
A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova street 32, Moscow 119991, Russia. amir@genome.eimb.relarn.ru
Kolchinsky
Alexander
A
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D004268:DNA-Binding Proteins / Q000032:analysis; D016678:Genome; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008832:Microchemistry; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D020543:Proteome / Q000032:analysis*
D004268:DNA-Binding Proteins; D020543:Proteome