title: Cerd4, third member of the d4 gene family: expression and organization of genomic locus.
abstract: Two members of the d4 family of presumptive transcription modulators, neuro-d4 (Neud4) and ubi-d4/Requiem (Req), have been characterized previously. We cloned and characterized the third member of this gene family, cer-d4 (Cerd4), from chicken and mouse cDNA libraries. The expression patterns of Cerd4 gene in both species are similar and more restricted than expression patterns of other two d4 genes. The main sites of Cerd4 expression are retina and cerebellum, where multiple transcripts could be detected. Two major types of Cerd4 proteins are a full-length isoform possessing all domains characteristic to the d4 family and truncated XZ isoform without C-terminal tandem of PHD fingers. The developmental kinetics of expression of these isoforms is different. The intron/exon structure of human Cerd4 gene is similar to that of neuro-d4 and ubi-d4/Requiem genes, but most introns of Cerd4 gene are much larger than the corresponding introns of the other two genes.
authors.affiliation: Department of Preclinical Veterinary Sciences, University of Edinburgh, Summerhall, Edinburgh, EH9 1QH, Scotland, UK.
authors.forename: N N
authors.initials: NN
authors.lastname: Ninkina
authors.forename: I B
authors.initials: IB
authors.lastname: Mertsalov
authors.forename: D A
authors.initials: DA
authors.lastname: Kulikova
authors.forename: M V
authors.initials: MV
authors.lastname: Alimova-Kost
authors.forename: O B
authors.initials: OB
authors.lastname: Simonova
authors.forename: L I
authors.initials: LI
authors.lastname: Korochkin
authors.forename: S L
authors.initials: SL
authors.lastname: Kiselev
authors.forename: V L
authors.initials: VL
authors.lastname: Buchman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D007438:Introns; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C452161:DPF3 protein, human; C486736:Dpf3 protein, mouse; D014157:Transcription Factors; D004247:DNA