Physical and transcript map of the hereditary prostate cancer region at xq27.
We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an approximately 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at approximately 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
Stephan
Dietrich A
DA
Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA. dstephan@cnmcresearch.org
Howell
Gareth R
GR
Teslovich
Tanya M
TM
Coffey
Alison J
AJ
Smith
Lorie
L
Bailey-Wilson
Joan E
JE
Malechek
Lindsay
L
Gildea
Derek
D
Smith
Jeffrey R
JR
Gillanders
Elizabeth M
EM
Schleutker
Johanna
J
Hu
Ping
P
Steingruber
Helen E
HE
Dhami
Pawandeep
P
Robbins
Christiane M
CM
Makalowska
Izabela
I
Carpten
John D
JD
Sood
Raman
R
Mumm
Steve
S
Reinbold
Rolland
R
Bonner
Tom I
TI
Baffoe-Bonnie
Agnes
A
Bubendorf
Lukas
L
Heiskanen
Mervi
M
Kallioneimi
Olli P
OP
Baxevanis
Andreas D
AD
Joseph
Shirin S
SS
Zucchi
Ileana
I
Burk
Robert D
RD
Isaacs
William
W
Ross
Mark T
MT
Trent
Jeffrey M
JM
D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D027042:Chromosomes, Artificial, P1 Bacteriophage / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000209:etiology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014960:X Chromosome / Q000235:genetics* / Q000648:ultrastructure
D012333:RNA, Messenger