Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.
We characterized the metastatic ability and mortality of four different mouse melanoma cell lines, B16-F0, -F1, -F10 and -BL6. B16-F0 is the parent cell line. B16-F1 was obtained by a one-time selective procedure and B16-F10 by a ten-time selective procedure using Fidler's method. B16-BL6 derived from B16-F10 has much more invasive activity than B16-F10. To investigate the difference in mortal malignancy among B16-F0, -F1, -F10 and -BL6, we examined the survival time of syngeneic C57BL/6Cr mice intravenously inoculated with these cells. As a control, we used the C57BL/6J-embryo mouse fibroblast-like semi-normal cell line. The ability to form lung metastatic nodules in mice gradually increased in the order: B16-F0, -F1, and -F10 (=-BL6). C57BL/6J-embryo cell (1 x 10(5)/mouse)-inoculated mice survived for over 46 days. B16-F0, -F1, -F10 and -BL6 (1 x 10(5)/mouse)-inoculated mice survived 31.4+/-4.4 (7), 25.7+/-2.8 (7), 23.6+/-1.5 (7) and 25.3+/-2.3 (7) days [mean+/-S.D. (number of mice)], respectively. According to the Mann-Whitney test, the B16-F0 inoculated group versus -F1 inoculated group (P<0.05), -F0 inoculated group versus -BL6 inoculated group (P<0.05), and -F0 inoculated group versus -F10 inoculated group (P<0.01) were significantly different, but the B16-F1 group versus -F10 group, -F1 group versus -BL6 group, and -F10 group versus -BL6 group were not. These results suggest that mortal malignancy is not necessarily correlated with lung-colonizing potential and even only one-time selected B16-F0 mouse melanoma cells are useful as an experimental metastatic model in vivo.
Nakamura
Kazuki
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan. lfp51193@mwu.mukogawa-u.ac.jp
Yoshikawa
Noriko
N
Yamaguchi
Yu
Y
Kagota
Satomi
S
Shinozuka
Kazumasa
K
Kunitomo
Masaru
M
D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D006403:Hematologic Tests; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000401:mortality / Q000556:secondary*; D008545:Melanoma / Q000401:mortality / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation / Q000473:pathology; D009929:Organ Size; D012878:Skin Neoplasms / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D015996:Survival Rate; D014407:Tumor Cells, Cultured