Discrimination of single-copy IS6110 DNA fingerprints of Mycobacterium tuberculosis isolates by high-resolution minisatellite-based typing.
Seven isoniazid-resistant isolates with mutations in the NADH dehydrogenase (ndh) gene were molecularly typed by IS6110-based restriction fragment length polymorphism analysis. All seven isolates with the R268H mutation had identical 1.4-kb IS6110 fingerprints. High-resolution minisatellite-based typing discriminated five of these isolates; two isolates were identical.
Lee
Ann S G
AS
Department of Clinical Research, Singapore General Hospital, Singapore 169608. dmslsg@nccs.com.sg
Tang
Lynn L H
LL
Lim
Irene H K
IH
Bellamy
Richard
R
Wong
Sin-Yew
SY
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting / Q000379:methods*; D004251:DNA Transposable Elements; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D004251:DNA Transposable Elements