Characteristics and potency of an acellular pertussis vaccine composed of pertussis toxin, filamentous hemagglutinin, and pertactin.
In an attempt to develop a safer pertussis vaccine, we successfully purified 3 pertussis protective antigens-pertussis toxin, filamentous hemagglutinin, and a 69-kDa outer membrane protein (also named pertactin), from Bordetella pertussis strain ATCC 9340. The toxicity of pertussis toxin could be effectively reduced by the treatment with formaldehyde 0.07% while preserving of a high degree of immunogenicity. By mixing purified pertussis antigens with diphtheria and tetanus toxoids (DT), we have formulated a DT acellular pertussis (DTaP) vaccine. Toxicity studies on body-weight gain in mouse, histamine sensitization, lymphocyte promoting, and Chinese hamster ovary cell clustering tests suggested that this DTaP vaccine is safer than a whole cell vaccine produced in France (DTP[F]). The formulated vaccine elicited high levels of anti-pertussis toxin antibodies in both mice and monkeys. In mice, a 2-fold neutralization of anti-pertussis toxin antibodies was produced by DTaP compared with DTP(F) vaccine and an acellular vaccine manufactured in Japan (DTaP[J]). More importantly, in intracerebral challenge assay in mouse, this vaccine also provided a better protection than DTaP(J).
Sheu
G C
GC
Vaccine Development Center, Taipei, Taiwan, ROC.
Wo
Y Y
YY
Yao
S M
SM
Chou
F Y
FY
Hsu
T C
TC
Ju
C L
CL
Cheng
Y
Y
Chang
S N
SN
Lu
C H
CH
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D001886:Bordetella pertussis / Q000737:chemistry; D004167:Diphtheria Toxin / Q000737:chemistry / Q000633:toxicity*; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology*; D006388:Hemagglutinins / Q000737:chemistry; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000737:chemistry; D013744:Tetanus Toxin / Q000737:chemistry / Q000633:toxicity*; D018675:Toxicity Tests; D022282:Vaccines, Acellular / Q000009:adverse effects* / Q000627:therapeutic use*; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins; D004167:Diphtheria Toxin; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D013744:Tetanus Toxin; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C068929:pertactin; D037342:Pertussis Toxin