Nitric oxide synthase upregulation and the predelivery blood pressure decrease in spontaneously hypertensive rats.
OBJECTIVE
The pregnant spontaneously hypertensive rat (SHR) exhibits a decrease in arterial blood pressure shortly before delivery; however, the mechanisms are unknown. Nitric oxide may be involved.


METHODS
Blood pressure in stroke-prone SHR (SHRSP) and Wistar-Kyoto control rats (WKY) was telemetrically measured. Four groups were studied: pregnant and non-pregnant WKY and SHRSP rats, respectively. Mean blood pressure in pregnant SHRSP rats decreased from 148 +/- 2 mmHg at conception to 120 +/- 4 mmHg at day 15, compared to 112 +/- 1 mmHg in pregnant WKY rats. At delivery, we determined the vasodilatory responses of isolated preconstricted aortic strips.


RESULTS
Vasodilatory responses from late-term SHRSP rats were significantly greater following acetylcholine than either those from non-pregnant SHRSP or pregnant and non-pregnant WKY rats (acetylcholine IC50: 5, 22.8, 398, 1000 nmol/l, respectively), while contractile responses to increasing doses of norepinephrine were not different. Similar results were obtained with substance P. Indomethacin had no effect on the relaxation responses. Relaxation in response to sodium nitroprusside was not different in the groups. Western blot analysis showed that endothelial nitric oxide synthase (eNOS) levels were significantly increased in the pregnant SHRSP vessels compared to non-pregnant SHRSP, pregnant WKY, and non-pregnant WKY vessels.


CONCLUSIONS
Increased NOS may explain the blood pressure decrease during late pregnancy in genetically hypertensive rats.
Gompf
Heinrich
H
HELIOS Klinikum-Berlin, Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charité, Humboldt University of Berlin, Germany.
Luft
Friedrich C
FC
Morano
Ingo
I
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology; D005260:Female; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D011247:Pregnancy / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*; D011921:Rats, Inbred WKY; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D019001:Nitric Oxide Synthase; D000109:Acetylcholine; D009638:Norepinephrine