The relevance of paternity analysis in Romanian population using the D1S80 locus.
At present, DNA fingerprinting for human identification and paternity testing is a necessary and usual procedure. D1S80 is one of the best known polymorphic loci showing a VNTR, and exhibiting a high heterozygosity. This genetic locus, with a Tsp 509 I polymorphism of its 5' flanking sequence (1, 9), have been successfully amplified from human genomic DNA isolated from blood. The Tsp 509 I polymorphism was detected by restriction after PCR amplification. We tested the relevance of paternity analysis using the D1S80 locus considering the allele frequency distribution characteristic for our country. Paternal and maternal bands were compared with the children's DNA patterns. Our data include a comparison between D1S80 alleles amplified from mother, child and the supposed father for three tested families. This study was the first of this type made in Romania. We concluded a good power of discrimination and exclusion for this locus. It can be used successfully in the case of subtypes with low frequencies, and this is frequent for our population because of the high heterozygosity of D1S80 subtypes in Romanian population. We recommend the D1S80 use for exclusion paternity tests in Romanian population, as a very useful molecular tool, but we also recommend a complete set of molecular markers for confirmation paternity test in the same population.
Ceacăreanu
A C
AC
Cantacuzino Institute, Bucharest, Romania.
Ceacăreanu
B
B
D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012383:Romania