Effects of posttraining treatments in the posterior cingulate cortex on short- and long-term memory for inhibitory avoidance in rats.
Adult male Wistar rats were bilaterally implanted with indwelling cannulae in the caudal region of the posterior cingulate cortex. After recovery, animals were trained in a step-down inhibitory avoidance task (3.0-s, 0.4-mA foot shock) and received, right after training, a 0.5-microl infusion of vehicle (phosphate-buffered saline, pH 7.4), of the GABA(A) receptor agonist muscimol (0.1 or 0.5 microg), of the cAMP-dependent protein kinase (PKA) stimulant Sp-cAMPS (0.1 or 0.5 microg), or of the PKA inhibitor Rp-cAMPS (0.1 or 0.5 microg). Animals were tested twice, 1.5 h and, again, 24 h after training, in order to examine the effects of these agents on short- and long-term memory, respectively. Muscimol (0.5 but not 0.1 microg) hindered retention for both short- and long-term memory (p <.05). Rp-cAMPS (0.1 or 0.5 microg) hindered retention for short-term memory (p <.05). In addition, these animals showed lower, but not significantly lower, latencies than controls in the test session for long-term memory (p >.10). A trend toward an amnesic effect on long-term memory was also observed after Sp-cAMPS infusion at 0.1 microg (p <.10). These results show that strong stimulation of GABAergic synapses in the caudal region of the rat posterior cingulate cortex right after training impairs short- and long-term memory (the latter less dramatically). The same occurs by inhibiting PKA activity with regard to STM and possibly to LTM.
Souza
M M
MM
Centro de Memória, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Ramiro Barcelos 2600, 90035-003 Porto Alegre, RS, Brazil. msouza@vortex.ufrgs.br
Mello e Souza
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T
Vinadé
E R
ER
Rodrigues
C
C
Choi
H K
HK
Dedavid e Silva
T L
TL
Medina
J H
JH
Izquierdo
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I
D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018755:GABA Agonists / Q000494:pharmacology; D006179:Gyrus Cinguli / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008568:Memory / Q000187:drug effects*; D008570:Memory, Short-Term / Q000187:drug effects; D017551:Microdialysis; D009118:Muscimol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D011963:Receptors, GABA-A / Q000187:drug effects; D012153:Retention, Psychology / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology
D004791:Enzyme Inhibitors; D018755:GABA Agonists; D011963:Receptors, GABA-A; D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D009118:Muscimol; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases