[The expression of laminin in the intermingled skin transplantation of allograft and autograft].
OBJECTIVE
To explore the healing mechanism of full-thickness wound treating by the intermingled skin transplantation of large sheet allograft with autograft through studying the expression of laminin (LN).


METHODS
Thirty-six SD rats with 10% to 15% of total body surface area (TBSA) full-thickness were made. After 3 days, the devitalized tissue were excised and transplanted a large sheet of allograft from Wistar rats and islets of autografts were implanted 3 days later. On day 3, 5, 7, 14, 21 after allografting, the expression of LN in the grafts were detected by immunohistochemistry.


RESULTS
On the 7th day postallografting, LN, which played positive action of epidermal cell adhesion, still retained in the allodermis after the rejection of alloepidermis occurred. On the 14th day postallografting, there appeared scattered LN underneath the epidermal cells migrating from islets of autografts. On the 21st day postallografting, LN in the basement membrane of skin grafts had completely formed.


CONCLUSIONS
The intermingled transplantation of large sheet allograft with autograft may provide components of basement membrane for wound healing, which may help to improve the appearance and function of skin.
Ding
Ren
R
Shanghai Burn Institute, Ruijin Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200025.
Jia
Sheng-xian
SX
Zhao
Yan-fei
YF
Chen
Yu-ying
YY
Yi
Jing
J
Tang
Xue-ming
XM
D000818:Animals; D005260:Female; D007797:Laminin / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism*; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014945:Wound Healing
D007797:Laminin