Lysophosphatidic acid induces glycodelin gene expression in cancer cells.
Glycodelin is a glycoprotein that has been suggested to be important in normal pregnancy and in malignancy. The regulation of its synthesis has not been studied. In this study, we report the induction of glycodelin gene expression by lysophosphatidic acid (LPA). We studied the effect of LPA (5, 10 and 25 microM) on glycodelin production in breast (MDA-MB-231), cervical (Hela), endometrial (RL-95), ovarian cancer (OVCAR-3) and erythroleukemia (K562) cells. There was a dose-dependent (5-25 microM) induction of glycodelin gene and protein expression in these cell types. LPA is a mimic of phorbol myristate acetate (PMA) action and is found to be elevated in high concentrations in the serum of cancer subjects. As glycodelin is an angiogenic protein with a potential immunosuppressive role, control of LPA synthesis might offer a potential target for intervention.
Ramachandran
Sumathi
S
Emory Center for Advanced Research on Women's Health, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA 30322, USA.
Ramaswamy
Sreemathy
S
Cho
Chi heum
Ch
Parthasarathy
Sampath
S
D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D011257:Pregnancy Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000071816:Glycodelin; D006023:Glycoproteins; D008246:Lysophospholipids; C045246:PAEP protein, human; D011257:Pregnancy Proteins