title: Do microtubules around the Toxoplasma gondii-containing parasitophorous vacuole in skeletal muscle cells form a barrier for the phagolysosomal fusion?
abstract: The intracellular fate of Toxoplasma gondii was studied in primary cultures of skeletal muscle cells (SMC). The labelling of secondary lysosomes with BSA-Au particles showed no phagolysosomal fusion with the vacuole containing the parasite. After internalization of the parasites, the parasitophorous vacuole became involved by closely apposed endoplasmic reticulum (ER) and mitochondria; within 18 h of interaction, microtubules were visualized in association with the parasitophorous vacuole, suggesting that they could form a barrier for the phagolysosomal fusion.
authors.affiliation: Department of Ultrastructure and Cellular Biology, Oswaldo Cruz Institute, FIOCRUZ, RJ, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Andrade
authors.forename: A C
authors.initials: AC
authors.lastname: Stumbo
authors.forename: L H
authors.initials: LH
authors.lastname: Monteiro-Leal
authors.forename: L
authors.initials: L
authors.lastname: Carvalho
authors.forename: H S
authors.initials: HS
authors.lastname: Barbosa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D008870:Microtubules / Q000469:parasitology* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000469:parasitology* / Q000648:ultrastructure; D010588:Phagosomes / Q000469:parasitology* / Q000648:ultrastructure; D014122:Toxoplasma / Q000502:physiology* / Q000648:ultrastructure; D014124:Toxoplasmosis, Animal / Q000503:physiopathology*; D014617:Vacuoles / Q000469:parasitology* / Q000648:ultrastructure