A proteomic approach to rapidly elucidate oligodendrocyte-associated proteins expressed in the myelinating rat optic nerve.
The lack of cDNA libraries derived from myelinating oligodendrocytes has hampered attempts to identify proteins associated with myelination during normal development or with remyelination after insult or disease. We, therefore, established a new method to elucidate such proteins by coupling the techniques of X-irradiation, two-dimensional (2-D) gel electrophoresis, and mass spectrometry. Specifically, 2-D gel protein profiles of normal optic nerves were compared with those of X-irradiated rat optic nerves, which were absent of oligodendrocytes, to elucidate oligodendrocyte-associated proteins. The subsequent identification of these oligodendrocyte-associated proteins was accomplished by mass spectrometry. The results presented in this paper demonstrate the potential of the X-irradiated optic nerve model system combined with proteomic techniques to rapidly elucidate oligodendrocyte-associated proteins expressed in vivo.
Colello
Raymond J
RJ
Department of Anatomy, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA. rcolello@hsc.vcu.edu
Fuss
Babette
B
Fox
Michael A
MA
Alberti
James
J
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D009413:Nerve Fibers, Myelinated / Q000737:chemistry / Q000528:radiation effects; D009836:Oligodendroglia / Q000737:chemistry* / Q000528:radiation effects; D009900:Optic Nerve / Q000737:chemistry / Q000166:cytology* / Q000528:radiation effects; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014965:X-Rays
D011506:Proteins; D020543:Proteome