Gene expression of MMP8 and MMP13 during embryonic development of bone and cartilage in the rat mandible and hind limb.
Matrix metalloproteinases (MMPs) 8 and 13 comprise the collagenase subfamily in rats and mice, and only MMP13 has been implicated in degradation of the collagenous matrices during development of bone and cartilage. On the hypothesis that MMP8 is also involved in bone and cartilage development, the present study was designed to investigate gene expression of MMP8 in rat embryonic mandibles and hind limbs. Expression of MMP8 was examined with in situ hybridization and RT-PCR and was compared with that of MMP13. Osteoblastic and chondrocytic cells expressing collagenous matrix molecules were identified using in situ hybridization for collagen Types I and II. The results demonstrated that MMP8 is expressed by osteoblastic progenitors, differentiated osteoblasts, osteocytes, and chondrocytes in the growth plate for the first time. Furthermore, the expression of MMP8 is much broader than that of MMP13, for which expression is confined to differentiated phenotypes of osteoblastic and chondrocytic lineage.
Sasano
Yasuyuki
Y
Division of Oral Molecular Biology, Tohoku University Graduate School of Dentistry, Sendai, Japan. sasano@anat.dent.tohoku.ac.jp
Zhu
Jing-Xu
JX
Tsubota
Makoto
M
Takahashi
Ichiro
I
Onodera
Kazuyuki
K
Mizoguchi
Itaru
I
Kagayama
Manabu
M
D000818:Animals; D001842:Bone and Bones / Q000196:embryology*; D002356:Cartilage / Q000196:embryology*; D019902:Chondrocytes / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics; D017364:Collagenases / Q000235:genetics*; D015870:Gene Expression; D005865:Gestational Age; D006614:Hindlimb / Q000196:embryology; D017403:In Situ Hybridization; D008334:Mandible / Q000196:embryology; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8 / Q000235:genetics*; D010006:Osteoblasts / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000378:metabolism
D024042:Collagen Type I; D024043:Collagen Type II; D017364:Collagenases; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat; D020784:Matrix Metalloproteinase 8; C000613587:Mmp8 protein, rat