Comparative analysis of sequences expressed during the liquid-cultured mycelia and fruit body stages of Pleurotus ostreatus.
To characterize genes involved in fruit body development, two complementary DNA (cDNA) libraries were constructed from RNA isolated from liquid-cultured mycelia and fruit bodies of Pleurotus ostreatus. Using single-pass sequencing of cDNA clones, 952 and 1069 expressed sequence tags (ESTs) were generated from liquid-cultured mycelia and fruit body cDNA library, respectively. A BLASTX search revealed that 390 of the liquid-cultured mycelia ESTs (41%) and 531 of the fruit body ESTs (50%) showed significant similarity to protein sequences described in the nonredudant database (E values < or =1 x 10(-5)). When liquid-cultured mycelia and fruit body ESTs were compared by the SeqMan II program, among the total of 2021 ESTs, 1256 ESTs were unigenes, and 66 unigenes (5.3%) were commonly expressed during both stages. The functional catalogs of the ESTs were made by comparison with functionally identified Saccharomyces cerevisiae genes. Liquid-cultured mycelium ESTs were compared with fruit body ESTs and changes of the expressed genes during fruit body development were analyzed.
Lee
Seung-Ho
SH
Applied Microbiology Division, Cytogenetics Division, National Institute of Agricultural Science and Technology, 249 Seodun-dong, Suwon, 441-707, South Korea.
Kim
Beom-Gi
BG
Kim
Kyung-Jin
KJ
Lee
Jin-Sung
JS
Yun
Doh-Won
DW
Hahn
Jang-Ho
JH
Kim
Gyu-Hyun
GH
Lee
Kang-Hyo
KH
Suh
Dong-Sang
DS
Kwon
Suk-Tae
ST
Lee
Chang-Soo
CS
Yoo
Young-Bok
YB
D003470:Culture Media; D018076:DNA, Complementary / Q000737:chemistry; D020224:Expressed Sequence Tags; D015723:Gene Library; D025282:Mycelium / Q000235:genetics; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development; D012331:RNA, Fungal / Q000737:chemistry; D017422:Sequence Analysis, DNA
D003470:Culture Media; D018076:DNA, Complementary; D012331:RNA, Fungal