Genomic diversity of human immunodeficiency virus type-1 in India.
Surveillance of HIV-1 subtypes has important implications for the development of candidate vaccine and understanding the possible differences in the transmission and natural history of different subtypes. In this study, HIV-1 subtypes were determined for homologies in the C2-V3-V5 region by heteroduplex mobility assay (HMA) in HIV-1 seropositive patients referred to the National HIV/AIDS Reference Centre, All India Institute of Medical Sciences in New Delhi, India. Of the 125 samples analysed, 98 (78.4%) were HIV-1 subtype C, 11 (8.8%) were subtype B', 3 (2.4%) were subtype A and 2 (1.6%) were subtype E. In 11 samples, subtype determination was not clear-cut. It is possible that these individuals may be infected with recombinant strains of HIV-1. These findings may have significant implications for the designing and testing of effective HIV-1 vaccine candidate in India.
Sahni
A K
AK
National HIV/AIDS Reference Centre, Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Dehli, India.
Prasad
V V S P
VV
Seth
P
P
D005260:Female; D014644:Genetic Variation; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D020180:Heteroduplex Analysis; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D010446:Peptide Fragments / Q000235:genetics
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments