Production of human papillomavirus type 16 E7 protein in Lactococcus lactis.
The E7 protein of human papillomavirus type 16 was produced in Lactococcus lactis. Secretion allowed higher production yields than cytoplasmic production. In stationary phase, amounts of cytoplasmic E7 were reduced, while amounts of secreted E7 increased, suggesting a phase-dependent intracellular proteolysis. Fusion of E7 to the staphylococcal nuclease, a stable protein, resulted in a highly stable cytoplasmic protein. This work provides new candidates for development of viral screening systems and for oral vaccine against cervical cancer.
Bermúdez-Humarán
L G
LG
Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
Langella
P
P
Miyoshi
A
A
Gruss
A
A
Guerra
R Tamez
RT
Montes de Oca-Luna
Roberto
R
Le Loir
Yves
Y
D003001:Cloning, Molecular; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D013294:Lactococcus lactis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D008836:Micrococcal Nuclease / Q000235:genetics / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics*; D050725:Papillomavirus E7 Proteins; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D003470:Culture Media; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D011993:Recombinant Fusion Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D008836:Micrococcal Nuclease