[The effects of epinephrine and adrenergic antagonists on adenosine 3', 5'-monophosphate level of bovine trabecular cells in vitro].
OBJECTIVE
To determine the effects of epinephrine (EPI) and adrenergic antagonists on adenosine 3', 5'-monophosphate (cAMP) level of bovine trabecular cells (BTC) in vitro.


METHODS
(3)H-cAMP was used in protein binding assay for measuring the intracellular level of cAMP.


RESULTS
(1) 10(-5) mol/L EPI induced a fold increase of cAMP in cultured BTC in vitro; (2) Timilol and ICI 118, 551 blocked efficiently the effect of EPI at a lower concentration (10(-6) mol/L). (3) Bisoprolol did not efficiently block the effect of EPI unless at high concentrations (>or= 10(-5) mol/L).


CONCLUSIONS
The effects of EPI increasing outflow facility may be associated with its increase of cAMP in trabecular cells; BTC contains beta-adrenergic receptors, and beta(2)-adrenergic receptors are dominant.
Lu
Y
Y
Department of Ophthalmology, The First Clinical Hospital, Beijing Medical University, Beijing 100034.
Li
M
M
Shen
Y
Y
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D007429:Intraocular Pressure / Q000187:drug effects; D014129:Trabecular Meshwork / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*
D000319:Adrenergic beta-Antagonists; D000242:Cyclic AMP; D004837:Epinephrine