Production of 8-OHdG and cytochrome c by cultured human mononuclear cells in patients with autoimmune thyroid disease.
Since oxidative stress is related to autoimmune thyroid disease, we studied the production of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and cytochrome c by culture of mononuclear cells from patients with Graves' disease and Hashimoto's thyroiditis. In patients with untreated Graves' disease, 8-OHdG and cytochrome c levels in culture supernatant of mononuclear cells were significantly higher than those of healthy control subjects, while the cytochrome c levels were significantly higher in patients with untreated Graves' disease and Hashimoto's thyroiditis than those of control subjects. Significant correlations between 8-OHdG and FT4, and cytochrome c were found. These results indicated that thyroid function has a potent influence on oxidative stress.
Hara
H
H
The 3rd Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.
Sato
R
R
Ban
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D034961:Antigens, Nuclear; D003574:Cytochrome c Group / Q000032:analysis / Q000096:biosynthesis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D006111:Graves Disease / Q000097:blood* / Q000201:enzymology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis / Q000096:biosynthesis; D013967:Thyroiditis, Autoimmune / Q000097:blood* / Q000201:enzymology; D013974:Thyroxine / Q000032:analysis / Q000096:biosynthesis
D034961:Antigens, Nuclear; D003574:Cytochrome c Group; D009687:Nuclear Proteins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D013974:Thyroxine