title: Anti-inflammatory and anti-tumor-promoting effects of cucurbitane glycosides from the roots of Bryonia dioica.
abstract: Seven new triterpene glycosides, bryoniosides A-G (1-7), have been isolated along with two known triterpene glycosides, cabenoside D (8) and bryoamaride (9), from a methanol extract of the roots of Bryoniadioica. The structures of 1-7 were determined on the basis of spectroscopic and chemical methods. Six compounds, 2, 3, 5, and 7-9, and 11 compounds, 1-9, bryodulcosigenin (10), and bryosigenin (11), respectively, were evaluated for their inhibitory effects on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation (1 microg/ear) in mice and on Epstein-Barr virus early antigen (EBV-EA) activation induced by TPA. All compounds tested showed marked anti-inflammatory effects, with 50% inhibitory doses (ID(50)) of 0.2-0.6 mg per ear. In addition, all of the compounds tested except for compound 5 showed potent inhibitory effects on EBV-EA induction (100% inhibition at 1 x 10(3) mol ratio/TPA).
authors.affiliation: College of Science and Technology, Nihon University, 1-8 Kanda Surugadai, Chiyoda-ku, Tokyo 101-8308, Japan.
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ukiya
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akihisa
authors.forename: Ken
authors.initials: K
authors.lastname: Yasukawa
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Masakazu
authors.initials: M
authors.lastname: Toriumi
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Kimura
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Nikaido
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoi
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.forename: Michio
authors.initials: M
authors.lastname: Takido
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000956:Antigens, Viral / Q000032:analysis; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029772:Bryonia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004423:Ear; D005260:Female; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000276:immunology; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D007564:Japan; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000956:Antigens, Viral; D000972:Antineoplastic Agents, Phytogenic; C033305:Epstein-Barr virus early antigen; D006027:Glycosides; D014315:Triterpenes; D013755:Tetradecanoylphorbol Acetate