[An experimental study of human trabecular cell growth on a filter and hydraulic conductivity].
OBJECTIVE
To search a method of culturing human trabecular meshwork cells (HTC) on a filter support and provide a model to study the hydraulic conductivity of HTC in vitro.


METHODS
The 3rd passage of HTC was cultured on a nylon filter, after that we measured the rate of different irrigation through the filter with HTC [Lp, microl/(min x mmHg x cm(2))].


RESULTS
HTC could continuously grow on the filter. The normal Lp was 10.45 microl/(min x mmHg x cm(2)). When the model was irrigated respectively by the solution containing epinephrine (EPI) and dexamethasone (DEX), the Lp value of HTC cultured in the same period was significantly higher than that of the normal group. However, after HTC exposed to DEX for a few days, Lp was significantly decreased.


CONCLUSIONS
(1) Better information of hydraulic conductivity and the effects of medicine on HTC can be obtained from the dynamic filter model; (2) EPI can improve the conductivity of HTC, and DEX can do the same in early period.
Dai
W
W
Department of Ophthalmology, First Hospital, Beijing Medical University, Beijing 100034.
Li
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D051381:Rats; D014129:Trabecular Meshwork / Q000166:cytology* / Q000502:physiology; D014872:Water Movements
D003907:Dexamethasone; D004837:Epinephrine