Normal development of serotonergic neurons in mice lacking S100B.
S100B, a glia-derived calcium binding protein, exhibits strong neurite extension activity in cultured serotonergic neurons. Using S100B-knockout mice, we examined whether this protein possesses in vivo serotonergic trophic activity. The distribution of serotonergic fibers, determined by immunohistochemistry, in the brains of S100B-knockout mice was quite similar to that of wild-type mice. Furthermore, the content of serotonin and its metabolite 5-hydroxyindole-3-acetic acid in knockout mice was also indistinguishable from those of wild-type mice. Our findings argue against the hypothesis that S100B has a crucial role in neurite extension of serotonergic neurons.
Nishiyama
Hiroshi
H
Laboratory for Behavioral Genetics, Brain Science Institute (BSI), RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Takemura
Masaaki
M
Takeda
Takehiro
T
Itohara
Shigeyoshi
S
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D001921:Brain / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D002135:Calcium-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D018345:Mice, Knockout; D009414:Nerve Growth Factors / Q000172:deficiency* / Q000235:genetics; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011903:Raphe Nuclei / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579159:S100b protein, mouse; D050486:Serotonin Plasma Membrane Transport Proteins; C493231:Slc6a4 protein, mouse; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid