[Study on structure change and hypermethylation of p16 gene in multiple myeloma].
OBJECTIVE
To illustrate the role of structure and hypermethylation of p16 gene in the pathogenesis of multiple myeloma(MM).


METHODS
By using PCR-single strand conformation polymorphisms(PCR-SSCP) and methylation-specific PCR(MSP) techniques, the structure and hypermethylation status of p16 gene in MM cell lines and patients were analysed.


RESULTS
Homozygous deletion of p16 exon 2 was found in KM3 cells. The completely methylated p16 gene and hypermethylation of CPG island were observed in U266, LP1 cell lines and 55.56% of MM patients.


CONCLUSIONS
Methylation of p16 gene is important in the pathogenesis of MM and may provide a new drug target for the treatment of MM.
Fu
W
W
Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Hou
J
J
Wang
D
D
D000368:Aged; D018899:CpG Islands / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005091:Exons; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured
D019941:Cyclin-Dependent Kinase Inhibitor p16