Preliminary research on myosin light chain kinase in rabbit liver.
OBJECTIVE
To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver.


METHODS
The expression of MLCK was detected by reverse transcription-polymerase chain reaction(RT-PCR); the MLCK was obtained from rabbit liver, and its activity was analyzed by gamma-(32)P incorporation technique to detect the phosphorylation of myosin light chain.


RESULTS
MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg x L(-1), the activity was at the highest level.


CONCLUSIONS
MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.
Ren
B
B
Department of Biochemistry and Molecular Biology, University of Science and Technology of China, Hefei 230027, Anhui Province, China.
Zhu
H Q
HQ
Luo
Z F
ZF
Zhou
Q
Q
Wang
Y
Y
Wang
Y Z
YZ
D000818:Animals; D022781:Hepatocytes / Q000201:enzymology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits
D018994:Myosin Light Chains; D009219:Myosin-Light-Chain Kinase