[A preliminary study on placenta hematopoiesis].
OBJECTIVE
To study the role of placenta on fetal hematopoiesis during embryo ontogeny, so as to find a new source of hematopoietic stem/progenitor cells (HSC/HPC) for clinical transplantation.


METHODS
Cord blood was collected separately from cord vein and artery. CD(34)(+) cells were detected by FACS, CFU-GM semisolid agar culture, and hematopoietic growth factors (HGFs) by ELISA. The results of HE stain and CD(34) McAb immunohistochemistry stain of placenta chorionic villi were investigated.


RESULTS
The CFU-GM yields and the percentage of CD(34)(+) and CD(34)(+)/CD(38)(minus sign) cells in cord vein were higher than that in artery. The cord vein contained larger amount of hematopoietic stimulating factors and similar level of hematopoietic inhibitory factors as compared with that in artery. Blood island, reticular cells and scattered CD(34)(+) cells were found in the mesenchyme of placenta chorionic villi.


CONCLUSIONS
The placenta might be an another hematopoietic organ in ontogeny. It could be applied to clinical hematopoietic cell transplantation.
Zeng
F
F
Department of Pediatrics, Affiliated Hospital of Shandong Medical University, Jinan 250012, China.
Shen
B
B
Wang
H
H
Shi
Q
Q
Hou
H
H
Ma
X
X
D018952:Antigens, CD34 / Q000032:analysis; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000502:physiology*; D011247:Pregnancy
D018952:Antigens, CD34