title: New lupane glycosides from Pulsatilla chinensis.
abstract: Two new lupane glycosides along with five known triterpenoids were isolated from the roots of Pulsatilla chinensis (Ranunculaceae). The structures of the new glycosides were determined to be 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside D, 6) and 3-O-[beta-D-glucopyranosyl(1-->4)][alpha-L-rhamnopyranosyl(1-->2)]-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside E, 7) by spectroscopic analysis and chemical methods. The compounds were evaluated for cytotoxic activities against K-562 human leukemia and HeLa cells.
authors.forename: Wencai
authors.initials: W
authors.lastname: Ye
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Zhang
authors.forename: Wendy W
authors.initials: WW
authors.lastname: Hsiao
authors.forename: Shouxun
authors.initials: S
authors.lastname: Zhao
authors.forename: Chun-Tao
authors.initials: CT
authors.lastname: Che
mesh_terms: D001681:Biological Assay; D002240:Carbohydrate Sequence; D002681:China; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D029626:Ranunculaceae; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes; D000094062:Betulinic Acid
chemical_list: D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C455124:pulsatilloside D; D000094062:Betulinic Acid; C455125:pulsatilloside E