High-performance liquid chromatographic determination of tocopherols in infant formulas.
A method for the simultaneous determination of alpha-tocopherol acetate and alpha-, delta-, and gamma-tocopherols by normal-phase high-performance liquid chromatography (HPLC) with a fluorescent detector in infant formula is proposed. The values obtained in the determination of the analytical parameters: linearity, precision, limit of detection and accuracy (analysis of a standard reference material, SRM 1846), confirm the quality of the method. The proposed method is useful for the determination of alpha-, delta-, and gamma-tocopherols and alpha-tocopherol acetate in infant formulas at a low cost and in a total time of 2 h.
Rodrigo
N
N
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Burjassot, Spain.
Alegría
A
A
Barberá
R
R
Farré
R
R
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D024505:Tocopherols / Q000032:analysis*
D024505:Tocopherols