Resolution improvement from 'in situ annealing' of copper nitrite reductase crystals.
Significant improvement of the resolution of copper nitrite reductase crystals was achieved by using the in situ annealing technique. The effective resolution limits increased by 1.5 A from 2.5 to 1.0 A, the mosaicity value decreased from 1.5 to 0.3 degree and the spot shape changed from elliptical to circular.
Ellis
Mark J
MJ
Faculty of Applied Science, De Montfort University, Leicester LE1 9BH, England.
Antonyuk
Svetlana
S
Hasnain
S Samar
SS
D003460:Crystallization; D018360:Crystallography, X-Ray; D017403:In Situ Hybridization; D008958:Models, Molecular; D009572:Nitrite Reductases / Q000737:chemistry*; D011487:Protein Conformation
D009572:Nitrite Reductases; C052695:nitrite reductase, copper-containing