Partial filling micellar electrokinetic chromatography optimization studies of ibuprofen, codeine and degradation products, and coupling to mass spectrometry.
Studies have been performed to evaluate whether an on-line partial filling-micellar electrokinetic chromatography (PF-MEKC) system could be applied to a recently developed MEKC method for the separation of ibuprofen, codeine and one of the degradation products. Attempts to couple the PF-MEKC system to MS have also been performed. SDS concentration, micellar zone length and concentration of acetonitrile in the buffer were optimized using factorial design. When a small micelle zone was injected directly after the sample introduction, the results improved markedly. The MS parameters have not been optimized, but the studies show promising results for the use of PF-MEKC-mass spectrometry for identification of the degradation products.
Stubberud
Karin
K
Analytical Development, AstraZeneca R&D Södertälje, Södertälje, Sweden. karin.stubberuf@astrazeneca.com
Forsberg
Anna
A
Callmer
Karin
K
Westerlund
Douglas
D
D000097:Acetonitriles / Q000494:pharmacology; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D003061:Codeine / Q000032:analysis* / Q000378:metabolism; D004867:Equipment Design; D007052:Ibuprofen / Q000032:analysis* / Q000378:metabolism; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D012967:Sodium Dodecyl Sulfate
D000097:Acetonitriles; D012967:Sodium Dodecyl Sulfate; D003061:Codeine; D007052:Ibuprofen; C032159:acetonitrile