title: A 3.1-kb genomic fragment of Bacillus subtilis encodes the protein inhibiting growth of Xanthomonas oryzae pv. oryzae.
abstract: OBJECTIVE
  To clone genes of Bacillus subtilis encoding peptides that inhibit the growth of Xanthomonas orzae pv. oryzae (Xoo).
  
  
  RESULTS
  A 3.1-kb DNA fragment from B. subtilis SO113 encoding peptides that inhibit the growth of Xoo (anti-Xoo, showing an inhibition zone) was isolated from a plasmid library of B. subtilis 6 GM15. Sequence analysis revealed that it contained three complete open reading frames (ORFs): ybcO, ybcS and a novel ORF designated ybcPQ. Deleting the last 96 bp of ybcS from the plasmid eliminated the anti-Xoo activity, suggesting that ybcS is required for producing the anti-Xoo activity. However, no anti-Xoo activity could be detected for the plasmid with ybcS alone. Further analysis showed that ybcO, at least, was also required to obtain the anti-Xoo activity.
  
  
  CONCLUSIONS
  A fragment of B. subtilis has been cloned that expresses an anti-Xoo activity that requires ybcS and ybcO.
  
  
  CONCLUSIONS
  These genes could be useful for the genetic engineering of resistance to rice bacterial diseases and for the design of new anti-Xoo biocontrol agents.
authors.affiliation: Department of Biotechnology, College of Life Sciences, Peking University, Beijing, China.
authors.forename: D
authors.initials: D
authors.lastname: Lin
authors.forename: L J
authors.initials: LJ
authors.lastname: Qu
authors.forename: H
authors.initials: H
authors.lastname: Gu
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014974:Xanthomonas / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D001426:Bacterial Proteins