Automated sample preparation and LC-MS for high-throughput ADME quantification.
Bioanalytical groups in the pharmaceutical industry provide quantitative data to support all stages of drug discovery. The increased use of 96-well plates and robotic liquid handling systems, the availability of robust triple quadruple mass spectrometers, and developments in chromatographic and samples preparation techniques, have all increased the rate at which this data can be generated. This review describes currently used methods and emerging technologies for automation of high-throughput quantitative bioanalysis. The focus is on recent applications of sample preparation and chromatography techniques compatible with detection by triple quadruple mass spectrometers.
O'Connor
Desmond
D
Merck Sharp & Dohme Research Laboratories, Department of Medicinal Chemistry (Drug Metabolism Section), Neuroscience Research Centre, Terlings Park, Eastwick Road, Harlow, Essex, CM20 2QR, UK. desmond_oconnor@merck.com
D000042:Absorption; D000818:Animals; D001331:Automation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010599:Pharmacokinetics; D011506:Proteins / Q000737:chemistry; D014018:Tissue Distribution
D004364:Pharmaceutical Preparations; D011506:Proteins