[Apoptosis as a measure of chemosensitivity to anti-cancer drugs in gynecological cancer].
OBJECTIVE
To examine apoptosis as a measure of chemosensitivity to anti-cancer drugs in gynecological cancer xenografted into nude mice.


METHODS
The TUNEL method was used to investigate the induction of apoptosis after exposure to anti-cancer drugs in thirteen gynecological cancers xenografted into nude mice. Moreover, in vivo sensitivities of gynecologic cancer to anti-cancer drugs were determined according to the protocol developed by Battle Laboratories, Columbus, Ohio USA. Both methods were examined for correlation.


RESULTS
The apoptosis index by the TUNEL method showed a correlation with the findings using the protocol developed by Battle Laboratories, Columbus, Ohio USA.


CONCLUSIONS
Our findings indicate the ability to determine the measure of chemosensitivity by the apoptosis index.
Fushiki
Hiroshi
H
Dept. of Obstetrics and Gynecology, Tonami General Hospital.
Yuuki
Hiroyoshi
H
Nakashima
Akitoshi
A
Fujimura
Masaki
M
Saito
Shigeru
S
Izumi
Rikuichi
R
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D000077146:Irinotecan; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin / Q000494:pharmacology; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000473:pathology*; D017239:Paclitaxel / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000473:pathology
D000970:Antineoplastic Agents; D016685:Mitomycin; D000077146:Irinotecan; D017239:Paclitaxel; D002945:Cisplatin; D002166:Camptothecin