title: Caveolin-1 expression in ovarian carcinoma is MDR1 independent.
abstract: We studied the role of caveolin-1 in tumor progression and prognosis in serous ovarian carcinoma and the association between caveolin-1 and MDR1 expression. The study involved immunohistochemical analysis for caveolin-1 and P-glycoprotein (P-gp) expression in 75 effusions and 90 solid lesions from ovarian and primary peritoneal carcinoma; in situ hybridization for MDR1 messenger RNA (mRNA) expression in 62 effusions and all 90 tumors; and reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 mRNA expression in 23 effusions. Immunohistochemical analysis localized caveolin-1 to the cell membrane in 43 effusions and 24 tumors. P-gp membrane expression was detected in 14 effusions and 11 tumors; MDR1 mRNA, in 20 effusions and 30 tumors. Caveolin-1 mRNA was expressed in 19 effusions. Caveolin-1 protein expression showed no association with that of P-gp protein or MDR1 mRNA. The expression of all markers was similar in carcinoma cells in pleural and peritoneal effusions. Caveolin-1 is a novel diagnostic marker for effusions; expression is moderately elevated in tumor cells in effusions, possibly owing to altered signal transduction and metabolism in cancer cells at this site. Expression seems MDR1 independent.
authors.affiliation: Department of Pathology, Norwegian Radium Hospital, Oslo.
authors.forename: Ben
authors.initials: B
authors.lastname: Davidson
authors.forename: Iris
authors.initials: I
authors.lastname: Goldberg
authors.forename: Vered
authors.initials: V
authors.lastname: Givant-Horwitz
authors.forename: Jahn M
authors.initials: JM
authors.lastname: Nesland
authors.forename: Aasmund
authors.initials: A
authors.lastname: Berner
authors.forename: Magne
authors.initials: M
authors.lastname: Bryne
authors.forename: Bjørn
authors.initials: B
authors.lastname: Risberg
authors.forename: Juri
authors.initials: J
authors.lastname: Kopolovic
authors.forename: Gunnar B
authors.initials: GB
authors.lastname: Kristensen
authors.forename: Claes G
authors.initials: CG
authors.lastname: Tropé
authors.forename: Gregg
authors.initials: G
authors.lastname: van de Putte
authors.forename: Reuven
authors.initials: R
authors.lastname: Reich
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis* / Q000235:genetics; D000328:Adult; D000368:Aged; D001202:Ascitic Fluid / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002277:Carcinoma / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D051242:Caveolin 1; D022461:Caveolins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000175:diagnosis; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000378:metabolism / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000473:pathology; D010997:Pleural Neoplasms / Q000378:metabolism* / Q000556:secondary; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D014408:Biomarkers, Tumor; C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D012333:RNA, Messenger