Relationship between genotype and phenotype of flagellin C in Salmonella.
OBJECTIVE
To discover the relationship between the genotype and antigen serotype of flagellin C among Salmonella strains.


METHODS
Fragment of Salmonella flagellin C in plasmid pLS408 was cloned, sequenced and compared with the corresponding sequence in other strains. Salmonella strains including two typhi strains, one paratyphoid strain, one enteritidis and one typhimurium strain were isolated from outpatients. Genome DNA was purified respectively from these clinical isolates, then the corresponding flagellin C fragment was amplified by polymerase chain reaction,and the amplification products were analyzed by agarose gel electrophoresis.


RESULTS
The cloned fragment includes 582 nucleotides encoding the variable region and partial conservative region of Salmonella flagellin C in plasmid pLS408. With comparison to the corresponding sequences reported previously, there is only a little difference from other strains with the same flagellar serotype in both nucleotide and amino acid level. Specific PCR products were amplified in Salmonella strains with flagellar serotype H-1-d including S. muenchen, typhi and typhimurium, but not in S. paratyphoid C or S. enteritidis strains.


CONCLUSIONS
In this experiment, the specificity of nucleotide sequence could be found in flagellin C central variable regions as it exists in flagellar serotypes in Salmonella. It may be helpful to developing a rapid, sensitive, accurate and PCR-based method to detect Salmonella strains with serotype H-1-d.
Ji
W S
WS
Chinese PLA Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China.
Hu
J L
JL
Qiu
J W
JW
Pan
B R
BR
Peng
D R
DR
Shi
B L
BL
Zhou
S J
SJ
Wu
K C
KC
Fan
D M
DM
D000595:Amino Acid Sequence / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D005408:Flagellin / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D010641:Phenotype; D020033:Protein Isoforms / Q000235:genetics; D012475:Salmonella / Q000235:genetics*; D013045:Species Specificity
D020033:Protein Isoforms; D005408:Flagellin