title: Inhibition of murine corneal allograft rejection by treatment with antibodies to CD80 and CD86.
abstract: The purpose of this study was to investigate the role of CD80 and CD86 costimulatory molecules in corneal allograft rejection. Anti-CD80 and anti-CD86 monoclonal antibodies (mAbs) were administered after orthotopic corneal allograft transplantation. Graft rejection was observed by biomicroscopy. Population and localization of CD80(+)and CD86(+)cells in the cornea, cervical lymph nodes, and spleen were examined by flow cytometry and immunohistochemistry. The combined use of anti-CD80 and anti-CD86 mAbs was effective in prolonging corneal allograft survival. In the untreated mice bearing rejected graft, many CD86(+)and CD80(+)cells were found around the host-graft junctional area in the cornea, and CD86(high)cells were found in the cervical lymph node and spleen. In contrast, few CD86(+)or CD80(+)cells were observed in the cornea, cervical lymph node, and spleen from the mice treated with anti-CD80/CD86 mAbs. These results demonstrated a significant role of CD80 and CD86 costimulatory molecules in corneal allograft rejection.
authors.affiliation: Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan.
authors.forename: Fumie
authors.initials: F
authors.lastname: Kagaya
authors.forename: Junko
authors.initials: J
authors.lastname: Hori
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Kamiya
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kaji
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Oshika
authors.forename: Shiro
authors.initials: S
authors.lastname: Amano
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamagami
authors.forename: Tadahiko
authors.initials: T
authors.lastname: Tsuru
authors.forename: Sumiyoshi
authors.initials: S
authors.lastname: Tanaka
authors.forename: Hironori
authors.initials: H
authors.lastname: Matsuda
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D018122:B7-1 Antigen / Q000032:analysis / Q000276:immunology*; D051940:B7-2 Antigen; D016039:Corneal Transplantation / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D006085:Graft Survival / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011184:Postoperative Period; D013154:Spleen / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins