[Effects of fasting on expression of insulin-like growth factor-I and IGF binding protein-1 genes in the liver of rats].
OBJECTIVE
To investigate the molecular mechanisms of body development and growth regulated by nutritional status.


METHODS
Effects of fasting on the expression of insulin-like growth factor-I (IGF-I) and IGF binding protein-1 (IGFBP-1) genes were analyzed by Northern blotting technique in rats.


RESULTS
Fasting for 48 hours, body weight of the rats decreased by 11.8%, level of blood glucose by 50.8% and content of IGF-I mRNA in the liver by 32.7% (P < 0.05). On the contrary, content of IGFBP-1 mRNA in the liver of fasted rats increased gradually, with a 2.5 time increase after 24 h fasting (P < 0.05). There was no significant change in the level of liver albumin mRNA after fasting.


CONCLUSIONS
Fasting could inhibit the expression of IGF-I gene and enhance the expression of IGFBP-1 gene.
Li
Q
Q
Department of Endocrinology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016.
Bréat
B
B
Czernichow
P
P
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015152:Blotting, Northern; D001835:Body Weight; D005215:Fasting; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009752:Nutritional Status; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D018970:Insulin-Like Growth Factor Binding Protein 1; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I