title: Immunolocalization studies of an antisense protein in HIV-1-infected cells and viral particles.
abstract: The minus DNA strand of HIV-1 presents an open reading frame that is complementary to the HIV-1 envelope messenger, is highly conserved among HIV-1 isolates, and may encode a hydrophobic protein. In previous studies, the antisense transcript has been identified both in various HIV-infected cell lines and in leukocytes of HIV-1(+) patients. The expression of the corresponding antisense protein (ASP) during natural HIV-1 infection has been indirectly evidenced by the identification of anti-ASP antibodies in the sera of HIV(+) patients. We have used immunoelectron microscopy procedures (ultra-small gold particles coupled to silver enhancement) to establish direct evidence of ASP production. ASP has then been detected in chronically and acutely HIV-1-infected cell lines. The protein, found mostly associated with various cellular membranes as well as with the virions released from the cells, indicated that ASP is a bona fide component for the virions and may participate in the particle formation.
authors.affiliation: INSERM U511, CHU Pitié-Salpêtrière, 91 boulevard de l'Hôpital, Paris 75013, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Briquet
authors.forename: Catherine
authors.initials: C
authors.lastname: Vaquero
mesh_terms: D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D015686:Gene Products, env / Q000235:genetics / Q000378:metabolism; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
chemical_list: D015686:Gene Products, env; D016372:RNA, Antisense; D014764:Viral Proteins