Morphologic phenotyping with MR microscopy: the visible mouse.
A method for rapid morphologic phenotyping is demonstrated by using magnetic resonance microscopy. Whole fixed C57BL/6J mice were imaged at 110-microm isotropic resolution; limited volumes of the intact specimen, at 50-microm isotropic resolution; and isolated organs, at 25-microm isotropic resolution. The three-dimensional imaging technique was applied to uricase knockout mice to demonstrate the method for the evaluation of morphologic phenotype.
Johnson
G Allan
GA
Center for In Vivo Microscopy, Duke University Medical Center, Rm 141, D Bryan Neuroscience Bldg, Research Dr, Durham, NC 27710, USA. gaj@orion.mc.duke.edu
Cofer
Gary P
GP
Gewalt
Sally L
SL
Hedlund
Laurence W
LW
D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D019786:Gadolinium DTPA; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice / Q000033:anatomy & histology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008853:Microscopy / Q000379:methods*; D010641:Phenotype; D014503:Urate Oxidase / Q000235:genetics
D003287:Contrast Media; D014503:Urate Oxidase; D019786:Gadolinium DTPA