Characterization of Symbiobacterium toebii, an obligate commensal thermophile isolated from compost.
A symbiotic thermophilic bacterium, strain SC-1, was isolated from hay compost (toebi) in Korea. The new isolate exhibited an obligate commensal interaction with a thermophilic Geobacillus strain and required crude extracts and/or culture supernatant from Geobacillus sp. SK-1 for axenic growth. The growth factors from Geobacillus sp. SK-1 were irreversibly inactivated by phenol or protease treatment, suggesting that they might be proteins. The cells of strain SC-1 were non-spore forming, nonmotile rods that were stained Gram-negatively. The isolate was a microaerophilic heterotroph. Growth was observed between 45 degrees and 70 degrees C (optimum: 60 degrees C; 2.4-h doubling time) and pH 6.0 and 9.0 (optimum: pH 7.5). The G+C content of the genomic DNA was 65 mol%, and the major quinones were MK-6 and MK-7. A phylogenetic analysis of its 16S rDNA sequence indicated that strain SC-1 is closely related to Symbiobacterium thermophilum and so was named Symbiobacterium toebii on the basis of its physiological and molecular properties.
Rhee
Sung-Keun
SK
Microbial Genomics Lab., Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Daejeon.
Jeon
Che Ok
CO
Bae
Jin-Woo
JW
Kim
Kwang
K
Song
Jae Jun
JJ
Kim
Joong-Jae
JJ
Lee
Seung-Goo
SG
Kim
Hong-Ik
HI
Hong
Seung-Pyo
SP
Choi
Yoon-Ho
YH
Kim
Su-Mi
SM
Sung
Moon-Hee
MH
D039903:Actinobacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006358:Hot Temperature; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010802:Phylogeny; D012037:Refuse Disposal
D004269:DNA, Bacterial