title: Gastric hyperplasia and parietal cell loss in Taenia taeniaeformis inoculated immunodeficient mice.
abstract: Immunodeficient mice were studied to determine their suitability as models in investigating the role of Taenia taeniaeformis larval products in the development of gastric hyperplasia. Recombinant active gene 2 (RAG2)-deficient and severe combined immune-deficient (SCID) mice were studied as candidate animal models. RAG2-deficient mice inoculated orally with T. taeniaeformis eggs developed gastric hyperplasia with alcian blue-periodic acid-Schiff-positive cell proliferation similar to those of rats. SCID mice inoculated with different doses and routes of T. taeniaeformis in vitro-hatched oncospheres and those orally inoculated with eggs resulted also in different degrees of gastric hyperplasia. Influence of inoculation forms of parasite, doses and routes of inoculation on initiation of hyperplastic gastropathy was suggested to be dependent on number and size of developed larvae. Both RAG2-deficient and SCID mice with hyperplastic mucosa were observed with significant loss of parietal cells. Apparent decrease in parietal cell number was observed in SCID mice at 2 weeks after intraperitoneal inoculation with oncospheres before hyperplastic lesions developed. Earliest occurrence of gastric hyperplasia in SCID mice was observed at 3 weeks after oral inoculation of in vitro-hatched oncospheres, sooner than orally inoculated rats. The results suggested that these immunodeficient mice could be used as animal models to study factors involved in T. taeniaeformis-induced gastric mucous cell hyperplasia.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
authors.forename: Jose Trinipil
authors.initials: JT
authors.lastname: Lagapa
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Konno
authors.forename: Yuzaburo
authors.initials: Y
authors.lastname: Oku
authors.forename: Nariaki
authors.initials: N
authors.lastname: Nonaka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.forename: Masao
authors.initials: M
authors.lastname: Kamiya
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000473:pathology*; D006801:Humans; D006965:Hyperplasia / Q000473:pathology*; D007814:Larva / Q000254:growth & development / Q000472:pathogenicity; D051379:Mice; D018345:Mice, Knockout; D016513:Mice, SCID; D009687:Nuclear Proteins; D010295:Parietal Cells, Gastric / Q000473:pathology*; D013621:Taenia / Q000254:growth & development / Q000472:pathogenicity*; D013622:Taeniasis / Q000469:parasitology / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2