title: Pulmonary granulomas of guinea pigs induced by inhalation exposure of heat-treated BCG Pasteur, purified trehalose dimycolate and methyl ketomycolate.
abstract: This study was designed to determine the identity of granulomatogenic substances in Mycobacterium bovis BCG Pasteur. When heat-treated BCG Pasteur bacilli were introduced into the lungs of guinea-pigs by an inhalation exposure apparatus, pulmonary granulomas without necrosis developed. Furthermore, when four kinds of mycolates derived from M. tuberculosis Aoyama B strain were introduced into the lungs by the same method, only trehalose 6,6'-dimycolate (TDM) and methyl ketomycolate induced pulmonary granulomas without central necrosis. The pulmonary granulomas consisted of epithelioid macrophages and lymphocytes. When a mixture of TDM and anti-TDM antibody was introduced into the lungs, development of granulomatous lesions was reduced. These data indicate that TDM and methyl ketomycolate are potent granulomatogenic reagents.
authors.forename: I
authors.initials: I
authors.lastname: Sugawara
authors.forename: T
authors.initials: T
authors.lastname: Udagawa
authors.forename: S C
authors.initials: SC
authors.lastname: Hua
authors.forename: M
authors.initials: M
authors.lastname: Reza-Gholizadeh
authors.forename: K
authors.initials: K
authors.lastname: Otomo
authors.affiliation: Department of Molecular Pathology, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, and *4th Department of Internal Medicine, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D003311:Cord Factors / Q000633:toxicity*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006099:Granuloma / Q000209:etiology*; D006168:Guinea Pigs; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000209:etiology*; D009163:Mycobacterium bovis / Q000276:immunology / Q000472:pathogenicity*; D009171:Mycolic Acids / Q000633:toxicity*
chemical_list: D003311:Cord Factors; D004269:DNA, Bacterial; D009171:Mycolic Acids