[Study on the relationship between apoptosis and reactive oxygen species of cancer cell lines induced by anticarcinogens].
OBJECTIVE
To investigate the relationship between apoptosis (AP) and fluorescent intensity of reactive oxygen species (ROS) in cancer cell lines induced by anticancer agents, and the mechanisms of drug sensitivity and resistance.


METHODS
K562 cells were treated respectively with each of the Vp16, ADR and DDP, the fluorescent intensity of ROS and AP percentage of K562 cells at different concentration of the drugs treated were assayed by flow cytometry (FCM).


RESULTS
(1) After treatment with these drugs for 24 hours, the fluorescent intensity of ROS and AP percentage of K562 cells increased obviously as compared with that of control groups. (2) The optimum concentrations of the drugs for ROS production and AP of K562 cells were determined.


CONCLUSIONS
Anticancer agents induced AP of K562 cells, which might be through stimulating ROS production.
Cai
L
L
China-Japan Union Hospital of Norman Bethune Medical University, Changchun 130031, China.
Gao
S
S
Yang
Y
Y
Zheng
D
D
Wang
W
W
Zhang
G
G
Fuyong
Q
Q
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D009369:Neoplasms / Q000378:metabolism* / Q000503:physiopathology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species