title: [The lipase/lipoxygenase bienzyme system in AOT reversed micelles in octane].
abstract: In this work it is shown that the bienzyme lipase/lipoxygenase system can function in reversed micelles of bis(2-ethyl)hexyl sulfosuccinate (AOT) in octane. As a lipase substrate, a fish fat preparation (fat of sea mammals) with a high content of polyunsaturated fatty acids was used. It was demonstrated that the bienzyme reaction proceeded in a stationary mode and had a rate-limiting step catalyzed by lipase. Under optimal conditions, the efficacy of functioning of the bienzyme system was by an order of magnitude higher than that in water. The lipase/lipoxygenase bienzyme system can be used as a new method of spectrophotometric determination of lipase activity. The English version of the paper.
authors.affiliation: Moscow State University, Chemical Faculty, Vorob'evy gory, GSP-3 Moscow, 119899 Russia.
authors.forename: I M
authors.initials: IM
authors.lastname: Pavlenko
authors.forename: O S
authors.initials: OS
authors.lastname: Kuptsova
authors.forename: N L
authors.initials: NL
authors.lastname: Kliachko
authors.forename: A V
authors.initials: AV
authors.lastname: Levashov
mesh_terms: D000818:Animals; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D005395:Fish Oils / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008084:Lipoxygenase / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D009795:Octanes / Q000737:chemistry*
chemical_list: D005395:Fish Oils; D008823:Micelles; D009795:Octanes; D004143:Dioctyl Sulfosuccinic Acid; D008084:Lipoxygenase; D008049:Lipase; C026728:octane