Evidence that nitric oxide is involved in autoregulation in optic nerve head of rabbits.
OBJECTIVE
To determine the role played by nitric oxide (NO) in the autoregulation of circulation in the optic nerve head (ONH).


METHODS
The intraocular pressure (IOP) was increased and maintained at 50 mm Hg by the infusion of balanced saline solution (BSS) into the anterior chamber of albino rabbits. Experiments were performed with or without an intravenous injection of 10, 20, or 50 mg/kg N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase (NOS) inhibitor. The blood flow in the ONH was evaluated by the hydrogen clearance method, and NO metabolites (nitrite and nitrate) were measured in the ONH under the same experimental conditions in other rabbits. Visual evoked potentials (VEPs) were recorded before the IOP elevation and every 15 minutes during the 60 minutes of elevation. The effect of elevated IOP on the VEPs and the hemodynamics and NO levels in the ONH were determined. The effect of pretreatment with a NOS inhibitor on the IOP-induced changes was also investigated.


RESULTS
The implicit time of the VEP was prolonged after L-NAME in a dose-dependent manner, whereas the implicit time in the control group (saline) was less affected. Blood flow in the ONH was not reduced by an elevation of the IOP (50 mm Hg) but was significantly reduced by L-NAME (20 mg/kg). The NO metabolites, which were elevated in the ONH during IOP elevation in the control, were also depressed by L-NAME pretreatment.


CONCLUSIONS
These results indicate that NO may play a role in the autoregulation of circulation in the ONH during elevated IOP. This would mean that NO provides some neuroprotection during an acute phase of ischemia in the ONH.
Okuno
Takashi
T
Department of Ophthalmology, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki, Osaka, 569-8686 Japan.
Oku
Hidehiro
H
Sugiyama
Tetsuya
T
Yang
Ying
Y
Ikeda
Tsunehiko
T
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005074:Evoked Potentials, Visual / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D007429:Intraocular Pressure; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009798:Ocular Hypertension / Q000503:physiopathology; D009898:Optic Disk / Q000098:blood supply*; D011817:Rabbits; D012039:Regional Blood Flow / Q000502:physiology
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester