A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection.
The immunogenicity and protective efficacy of overlapping regions of the protective antigen (PA) polypeptide, cloned and expressed as glutathione S-transferase fusion proteins, have been assessed. Results show that protection can be attributed to individual domains and imply that it is domain 4 which contains the dominant protective epitopes of PA.
Flick-Smith
Helen C
HC
Dstl, Chemical and Biological Sciences, Porton Down, Salisbury, Wiltshire SP4 0JQ, United Kingdom.
Walker
Nicola J
NJ
Gibson
Paula
P
Bullifent
Helen
H
Hayward
Sarah
S
Miller
Julie
J
Titball
Richard W
RW
Williamson
E Diane
ED
D000818:Animals; D000881:Anthrax / Q000401:mortality / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000276:immunology*; D000942:Antigens, Bacterial; D001427:Bacterial Toxins / Q000276:immunology*; D005260:Female; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology; D017434:Protein Structure, Tertiary; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
D022122:Anthrax Vaccines; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D010446:Peptide Fragments; D014614:Vaccines, Synthetic; C030325:anthrax toxin