title: Successful pregnancy with intrauterine insemination using vasal sperm retrieved by electric stimulation.
abstract: OBJECTIVE
  To determine the feasibility of electric stimulation applied to the epididymis or vas deferens to retrieve vasal sperm.
  
  
  METHODS
  Two case reports.
  
  
  METHODS
  Assisted reproduction practice in a hospital.
  
  
  METHODS
  Two patients with retrograde ejaculation and severe asthenozoospermia.
  
  
  METHODS
  Pulse electric simulation was applied to the epididymis or vas deferens to retrieve sperm via a tube cannulated into the vas deferens. After diluting with culture medium, retrieved sperm were used for IUI.
  
  
  METHODS
  The volume of retrieved vasal fluid and motility parameters of the sperm, the achievement of pregnancy, and outcome of pregnancy.
  
  
  RESULTS
  In patient 1, the volumes of vasal fluids and numbers of sperm were 10-40 microL and 31.4-75.9 x 10(6), respectively, during two cycles. Sperm motility was 88.4%--93.2%. His wife became pregnant and was delivered of a healthy infant by IUI during the second cycle. In patient 2, 0.6 x 10(6) sperm with a motility of 70% were retrieved and used for IUI. However, his wife did not become pregnant.
  
  
  CONCLUSIONS
  Electric vasal sperm retrieval is a feasible method for collecting sperm from the vas deferens in selected patients with male factor infertility.
authors.affiliation: Department of Urology, Yokohama City University, Yokohama, Japan. zxb07166@nifty.ne.jp
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saito
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: Kotaro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sato
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuura
mesh_terms: D000328:Adult; D004599:Electric Stimulation Therapy; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007248:Infertility, Male / Q000628:therapy*; D007315:Insemination, Artificial; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D014649:Vas Deferens / Q000601:surgery*