Lack of a consistent relationship between demethylation of the CD44 promoter and CD44 expression.
Methylation of the CD44 promoter at one or more CpG dinucleotides has been proposed as being important in the control of CD44 expression. In vitro methylation of all CpGs occurring between bases -1 to -1374 upstream of the position of translation initiation repressed the promoter activity of mouse CD44 4.5- to 12-fold in transient transfection experiments. Assaying the methylation of these 29 CpGs by genomic sequencing using differential base modification by sodium bisulfite indicated that a cluster of three CpGs immediately upstream of the position of translation initiation was heavily methylated in the mouse CD44-negative T-cell lymphoma AKR1. All 19 CpGs between bases -4 and -447, including the cluster heavily methylated in AKR1, were demethylated in the CD44-positive T-cell lymphoma BW5147, while CpGs further upstream showed no change in methylation pattern. The cluster of heavily methylated CpGs remained methylated, however, when CD44 was activated by transient transfection of c-jun into an AKR1 subline expressing polyoma large-T or by treatment of this subline with sodium butyrate, and no significant demethylation of other CpGs was observed. It is concluded, therefore, that no consistent demethylation event in the promoter-containing region encompassing the 1374 base pairs upstream of the position of CD44 translation initiation is required for CD44 expression.
Hyman
Robert
R
Molecular and Cell Biology Laboratory, Salk Institute, Box 85800, San Diego, CA 92186-5800, USA. rhyman@dcbsun.salk.edu
D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D020148:Butyric Acid / Q000494:pharmacology; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects; D000077209:Decitabine; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D018960:Hyaluronan Receptors / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D018960:Hyaluronan Receptors; D016755:Proto-Oncogene Proteins c-jun; D020148:Butyric Acid; D000077209:Decitabine; D001374:Azacitidine