Unexpected stability of mariner transgenes in Drosophila.
A number of mariner transformation vectors based on the mauritiana subfamily of transposable elements were introduced into the genome of Drosophila melanogaster and examined for their ability to be mobilized by the mariner transposase. Simple insertion vectors were constructed from single mariner elements into which exogenous DNA ranging in size from 1.3 to 4.5 kb had been inserted; composite vectors were constructed with partial or complete duplications of mariner flanking the exogenous DNA. All of the simple insertion vectors showed levels of somatic and germline excision that were at least 100-fold lower than the baseline level of uninterrupted mariner elements. Although composite vectors with inverted duplications were unable to be mobilized at detectable frequencies, vectors with large direct duplications of mariner could be mobilized. A vector consisting of two virtually complete elements flanking exogenous DNA yielded a frequency of somatic eye-color mosaicism of approximately 10% and a frequency of germline excision of 0.04%. These values are far smaller than those observed for uninterrupted elements. The results imply that efficient mobilization of mariner in vivo requires the presence and proper spacing of sequences internal to the element as well as the inverted repeats.
Lozovsky
Elena R
ER
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Nurminsky
Dmitry
D
Wimmer
Ernst A
EA
Hartl
Daniel L
DL
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D009030:Mosaicism / Q000235:genetics; D019076:Transgenes; D019895:Transposases
D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases