[The effect of viper venom on the attachment, migration and proliferation of Tenon's capsular fibroblasts].
OBJECTIVE
To observe the effect of viper (Ahylysantipfarciasi) venom on attachment to collagen, migration and proliferation of rabbit Tenon's capsular fibroblast (TFs) in tissue culture.


METHODS
Anti-adhesion experiment: The 3 -- 5 passage TFs suspended in DMEM medium were pre-incubated for 30 minutes in the presence of various concentrations of viper venom (0, 2.5 x 10(-4), 5.0 x 10(-4), 1.0 x 10(-3) and 5.0 x 10(-3) U/ml) at 37C, 5% CO(2), then inoculated in 24-well plate coated with rat-tail collagen. After incubation for 90 minutes, the floating cells were removed. The attached cells in each well were enumerated microscopically and determined by the value of absorption (A) of 3-(4, 5-dimethylthiazolzyl)-2, 5-diphenyl tetrazodium bromide (MTT). Anti-migration experiment: A denuded area was made when the 3rd passage of TFs was confluent into a monolayer. Cells were then exposed to viper venom (0 -- 5.0 x 10(-3) U/ml) at 37C, 5% CO(2), and the cells having migrated into the denuded area were enumerated every 6 hours. Anti-proliferative experiment: Two hours after the 3rd passage of TFs was incubated in the 24-well plate at 37C, 5% CO(2), they were exposed to different concentrations of viper venom drug (0 -- 5.0 x 10(-3) U/ml). Twenty-four and 48 hours later, the number of cells was determined by MTT method.


RESULTS
Viper venom inhibited TFs from attaching to collagen in a dose-dependent manner and the ID(50) was 1.0 x 10(-3) U/ml. Only did 5.0 x 10(-3) U/ml of viper venom show significant difference from the control during 6 -- 48 hours. The difference of A value was not significant among all groups at 24 and 48 hr.


CONCLUSIONS
In vitro, viper venom (> 1.0 x 10(-3) U/ml) can significantly inhibit the attachment of TFs to collagen, and 5.0 x 10(-3) U/ml can inhibit the migration, but can not affect their proliferation.
Liu
J
J
Department of Ophthalmology, First Affiliated Hospital, Guangxi Medical University, Nanning 530020, China.
Wei
H
H
Lu
Y
Y
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D003228:Conjunctiva / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008297:Male; D011817:Rabbits; D013997:Time Factors; D014757:Viper Venoms / Q000494:pharmacology*
D014757:Viper Venoms