title: The 5-series F(2)-isoprostanes possess no vasomotor effects in the rat thoracic aorta, the human internal mammary artery and the human saphenous vein.
abstract: 1. Among the F(2)-isoprostanes, the 15- and the 5-series are currently used as markers of lipid peroxidation in vascular diseases. 15-F(2t)-IsoP (also named iPF(2 alpha)-III) exerts a vasoconstriction in most vessels, whereas no data is available concerning 5-F(2t)-IsoP (also named iPF(2 alpha)-VI), which is more abundant in plasma. 2. The aim of this study was to determine whether 5-F(2t)-IsoP possess any vascular effects on various vessels including the isolated rat thoracic aorta, the human internal mammary artery and the saphenous vein. 3. In organ baths, 5-F(2t)-IsoP and its 5-epimer did not affect the basal tone of any vessel, unlike 15-F(2t)-IsoP. These compounds possessed no antagonist effects on 15-F(2t)-IsoP-induced contractions, No dilator effect was observed in comparison with sodium nitroprusside and acetylcholine on the rat aorta. 4. In conclusion, we show that unlike 15-F(2t)-IsoP, 5-F(2t)-IsoP and its 5-epimer possess no vasomotor effects and as such are unlikely to be involved in the pathogenesis of vascular diseases. Further studies are required to test whether these mediators may have effects on systems not being measured in the current study.
authors.affiliation: Laboratoire de Pharmacologie, LSCPA EA2937, Faculté de Médecine et Centre Hospitalier de Grenoble, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Marlière
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cracowski
authors.forename: Thierry
authors.initials: T
authors.lastname: Durand
authors.forename: Olivier
authors.initials: O
authors.lastname: Chavanon
authors.forename: Janine
authors.initials: J
authors.lastname: Bessard
authors.forename: Alexandre
authors.initials: A
authors.lastname: Guy
authors.forename: Françoise
authors.initials: F
authors.lastname: Stanke-Labesque
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Rossi
authors.forename: Germain
authors.initials: G
authors.lastname: Bessard
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects*; D015415:Biomarkers; D015237:Dinoprost / Q000031:analogs & derivatives*; D004305:Dose-Response Relationship, Drug; D028441:F2-Isoprostanes / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008323:Mammary Arteries / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012501:Saphenous Vein / Q000187:drug effects*; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: C463000:5-F(2t)-isoprostane; D015415:Biomarkers; D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost