Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR.
A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.
Nagao
Shoji
S
Department of Obstetrics and Gynecology, Okayama University Medical School, Okayama, Japan.
Yoshinouchi
Mitsuo
M
Miyagi
Yasunari
Y
Hongo
Atsushi
A
Kodama
Junichi
J
Itoh
Sachio
S
Kudo
Takafumi
T
D015139:Blotting, Southern; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology
D004279:DNA, Viral