[Effect of transforming growth factor - beta1 on expression of CD69(+) and CD25(+) T cell induced by allo - corneal epithelial cells in vitro].
OBJECTIVE
To study the effect of transforming growth factor - beta(1) (TGF-beta1) on changes of CD(69)(+) and CD(25)(+) T cell population induced by allo - corneal epithelial cells in culture.


METHODS
The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was identified by using indirect immune fluorescence flow cytometry analysis system.


RESULTS
The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was significantly increased after peripheral blood had been cultured with allo - corneal epithelial cells for 3 hours However, the expression of CD(69)(+) was significantly decreased while TGF - beta(1) was added to the culture.


CONCLUSIONS
TGF - beta(1) significantly inhibits the expression of CD(69)(+) and CD(25)(+) in peripheral T cells induced by allo-corneal epithelial cells in vitro.
Wu
J
J
Department of Ophthalmology, Medical College, Jinan University, Guangzhou 510632, China. zxk@gdnmc.guangzhou.qd.cn
Xu
J
J
D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002478:Cells, Cultured; D003315:Cornea / Q000502:physiology*; D016039:Corneal Transplantation / Q000276:immunology; D004847:Epithelial Cells / Q000502:physiology; D006801:Humans; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013601:T-Lymphocytes / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000099192:CD69 Antigens; C508960:TGFB1 protein, human