title: Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptors.
abstract: Changes in mRNA and protein expression profiles of Shewanella oneidenesis MR-1 during switch from aerobic to fumarate-, Fe(III)-, or nitrate-reducing conditions were examined using DNA microarrays and two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In response to changes in growth conditions, 121 of the 691 arrayed genes displayed at least a two-fold difference in transcript abundance as determined by microarray analysis. Genes involved in aerobic respiration encoding cytochrome c and d oxidases and TCA cycle enzymes were repressed under anaerobic conditions. Genes induced during anaerobic respiration included those involved in cofactor biosynthesis and assembly (moaACE, ccmHF, nosD, cysG), substrate transport (cysUP, cysTWA, dcuB), and anaerobic energy metabolism (dmsAB, psrC, pshA, hyaABC, hydA). Transcription of genes encoding a periplasmic nitrate reductase (napBHGA), cytochrome c552, and prismane was elevated 8- to 56-fold in response to the presence of nitrate, while cymA, ifcA, and frdA were specifically induced three- to eightfold under fumarate-reducing conditions. The mRNA levels for two oxidoreductase-like genes of unknown function and several cell envelope genes involved in multidrug resistance increased two- to fivefold specifically under Fe(III)-reducing conditions. Analysis of protein expression profiles under aerobic and anaerobic conditions revealed 14 protein spots that showed significant differences in abundance on 2-D gels. Protein identification by mass spectrometry indicated that the expression of prismane, dihydrolipoamide succinyltransferase, and alcaligin siderophore biosynthesis protein correlated with the microarray data.
authors.affiliation: Environmental Sciences Division, Oak Ridge National Laboratory, Tennessee 37831-6038, USA.
authors.forename: Alex S
authors.initials: AS
authors.lastname: Beliaev
authors.forename: Dorothea K
authors.initials: DK
authors.lastname: Thompson
authors.forename: Tripti
authors.initials: T
authors.lastname: Khare
authors.forename: Hanjo
authors.initials: H
authors.lastname: Lim
authors.forename: Craig C
authors.initials: CC
authors.lastname: Brandt
authors.forename: Guangshan
authors.initials: G
authors.lastname: Li
authors.forename: Alison E
authors.initials: AE
authors.lastname: Murray
authors.forename: John F
authors.initials: JF
authors.lastname: Heidelberg
authors.forename: Carol S
authors.initials: CS
authors.lastname: Giometti
authors.forename: John
authors.initials: J
authors.lastname: Yates
authors.forename: Kenneth H
authors.initials: KH
authors.lastname: Nealson
authors.forename: James M
authors.initials: JM
authors.lastname: Tiedje
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhoui
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001692:Biological Transport; D004269:DNA, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D020592:Shewanella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial