title: Effects of heat and high-pressure treatments on antigenicity of beef extract.
abstract: The sera of bovine gamma globulin (BGG) positive beef allergic patients were used in this study in order to investigate changes in IgE-specific binding activity with regard to beef extract altered by heat or high-pressure treatment. In inhibition-ELISA, the sample treated at 60 degrees C did not show any significant changes in the antigenicity of BGG, but the sample treated at 100 degrees C showed a decrease of the antigenicity. In the case of the treatment with heating at 100 degrees C, heat-coagulation occurred in the beef extract. The resulting supernatant and precipitate of the sample by centrifugation were analyzed by immunoblotting. Only the fraction of precipitate showed a specific binding activity with the sera. Based on this result, it was speculated that the persistent antigenicity found even after the treatment at 100 degrees C in inhibition-ELISA remained principally in the heat-coagulated fraction, which indicated the importance of the method of handling the heat-coagulation in heat treatment. High-pressure treatments (200 MPa-600 MPa) of beef extract did not show any significant changes in the binding with the sera.
authors.affiliation: Doctor's Program in Functional Biology, Graduate School of Science and Technology, University of Niigata, Japan.
authors.forename: Gi Dong
authors.initials: GD
authors.lastname: Han
authors.forename: Masatomo
authors.initials: M
authors.lastname: Matsuno
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Ikeuchi
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D006361:Heating; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008460:Meat; D011312:Pressure; D014020:Tissue Extracts; D005719:gamma-Globulins / Q000276:immunology*
chemical_list: D000485:Allergens; D014020:Tissue Extracts; D005719:gamma-Globulins; D007073:Immunoglobulin E