Bacillus cereus cellulitis from contaminated heroin.
Concern exists over recent unexplained deaths among intravenous drug users. This report describes a patient with crepitant cellulitis who was admitted complaining of severe pain in the right forearm. Ultrasonography demonstrated gas in the tissues and he was referred for early surgical debridement of the arm. He was treated with intravenous benzyl penicillin, gentamicin and metronidazole and made a full recovery. Aspirate samples grew Bacillus cereus, morphologically similar to the isolate obtained from a sample of the patient's own heroin. Antibiogram and API 50CHB profiles were also similar. Further typing included 'H' flagellar serotyping, which found both blood and heroin strains to be non-typable, and amplified fragment polymorphism analysis, which showed that the strains were indistinguishable. Genotyping of two selected genes from B. cereus confirmed almost certain identity between the two strains. This case illustrates the potential virulence of B. cereus when inoculated into tissues, and to our knowledge, is the first report to demonstrate a conclusive microbiological link between contaminated heroin and serious sepsis in a drug user due to B. cereus.
Dancer
S J
SJ
McNAIR
D
D
Finn
P
P
Departments of Microbiology and Surgery*, Vale of Leven Hospital, Alexandria, Dunbartonshire, G83 OUA and †Biotechnology Centre of Oslo and Institute of Pharmacy, University of Oslo, 1125 Blindern, 0316 Oslo, Norway.
Kolsto
A-B
AB
Departments of Microbiology and Surgery*, Vale of Leven Hospital, Alexandria, Dunbartonshire, G83 OUA and †Biotechnology Centre of Oslo and Institute of Pharmacy, University of Oslo, 1125 Blindern, 0316 Oslo, Norway.
D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D002481:Cellulitis / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D005542:Forearm / Q000000981:diagnostic imaging; D005838:Genotype; D005839:Gentamicins / Q000627:therapeutic use; D003932:Heroin; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D008795:Metronidazole / Q000627:therapeutic use; D010146:Pain / Q000000981:diagnostic imaging / Q000209:etiology; D010406:Penicillins / Q000627:therapeutic use; D015819:Substance Abuse, Intravenous / Q000150:complications*; D014463:Ultrasonography
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D010406:Penicillins; D008795:Metronidazole; D003932:Heroin