[The significance of the adhesion of CD(34)(+) stem/progenitor cells to fibronectin in the pathogenesis of chronic myelogenous leukemia].
OBJECTIVE
To investigate the significance of the adhesion of CD(34)(+) stem/progenitor cells to fibronectin in the pathogenesis of chronic myelogenous leukemia (CML).


METHODS
(1) Integrin beta(1) chain (CD(29)) and alpha(4) chain (CD(49d)) expressions on CD(34)(+) cells were measured by flow cytometry in 30 untreated CML patients in chronic phase (CML-CP) and 10 healthy donors. (2) The adhesion function of immunomagnetic beads selected CD(34)(+) cells to fibronectin was detected by crystal violet staining in 5 untreated CML-CP patients and 5 healthy donors. (3) The effect of fibronectin on CFU-GM colony formation of bone marrow cells in 3 CML patients and 3 healthy donors was observed by limited dilution liquid microculture.


RESULTS
(1) There was no significant difference in CD(29) and CD(49d) expressions on CD(34)(+) bone marrow cells between CML patients and healthy donors. (2) The adhesion of CD(34)(+) bone marrow cells to fibronectin decreased significantly in CML patients as compared with that in healthy donors (P < 0.01). (3) The CFU-GM colony formation was significantly inhibited by fibronectin in healthy donors (P < 0.01), but not in CML patients.


CONCLUSIONS
The adhesion function abnormality of CML CD(34)(+) bone marrow cells to fibronectin may underlie the abnormal proliferation of CML progenitors.
Li
Z
Z
Institute of Hematology, People's Hospital, Beijing Medical University, Beijing 100034, China.
Li
J
J
Fu
J
J
Chen
S
S
D018952:Antigens, CD34 / Q000032:analysis*; D002448:Cell Adhesion; D005353:Fibronectins / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D039441:Integrin alpha4 / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000209:etiology*
D018952:Antigens, CD34; D005353:Fibronectins; D019012:Integrin beta1; D039441:Integrin alpha4