Ligand-inducible transgene regulation for gene therapy.
A synthetic ligand regulable system for gene transfer and expression has been developed in our laboratory based on mechanistic studies of steriod hormone receptor and transcriptional regulation. This gene switch system possesses most of the important features that are required for application of the system in biological research and clinical gene therapy in the future. As the primary ligand tested in this system, mifepristone can effectively turn on the regulatory circuit at doses much lower than those used in the clinic. By modification of the chimeric regulator and its feedback regulatory mode, this system has been optimized to produce very low basal activity with high inducibility in the presence of mifepristone. Also, improvements in regulator composition have been made to minimize immunogenicity and make the system more amenable to human gene therapy. Moreover, incorporation of this gene switch system into the HC-Ad vector system has further enhanced the efficiency of gene transfer and the long-term inducible expression of transgenes. However, for each application within a different biological system, the gene switch needs to be optimized to achieve appropriate inductions. In particular, the method used to deliver the transgenes and adjustment of ligand dosage are critical for in vivo gene expression.
Ye
Xiangcang
X
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Schillinger
Kurt
K
Burcin
Mark M
MM
Tsai
Sophia Y
SY
O'Malley
Bert W
BW
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005786:Gene Expression Regulation / Q000187:drug effects; D015316:Genetic Therapy; D005822:Genetic Vectors; D013006:Growth Hormone; D006801:Humans; D008024:Ligands; D051379:Mice; D008822:Mice, Transgenic; D015735:Mifepristone / Q000494:pharmacology; D019076:Transgenes
D017931:DNA Primers; D008024:Ligands; D015735:Mifepristone; D013006:Growth Hormone