Myristyl and palmityl acylation of pI 5.1 carboxylesterase from porcine intestine and liver.
Immunoblotting analyses revealed the presence of carboxylesterase in the porcine small intestine, liver, submaxillary and parotid glands, kidney cortex, lungs and cerebral cortex. In the intestinal mucosa, the pI 5.1 enzyme was detected in several subcellular fractions including the microvillar fraction. Both fatty monoacylated and diacylated monomeric (F1), trimeric (F3) and tetrameric (F4) forms of the intestinal protein were purified here for the first time by performing hydrophobic chromatography and gel filtration. The molecular mass of these three enzymatic forms was estimated to be 60, 180 and 240 kDa, respectively, based on size-exclusion chromatography and SDS/PAGE analysis. The existence of a covalent attachment linking palmitate and myristate to porcine intestinal carboxylesterase (PICE), which was suggested by the results of gas-liquid chromatography (GLC) experiments in which the fatty acids resulting from alkali treatment of the protein forms were isolated, was confirmed here by the fact that [3H]palmitic and [3H]myristic acids were incorporated into porcine enterocytes and hepatocytes in cell primary cultures. Besides these two main fatty acids, the presence of oleic, stearic, and arachidonic acids was also detected by GLC and further confirmed by performing radioactivity counts on the 3H-labelled PICE forms after an immunoprecipitation procedure using specific polyclonal antibodies, followed by a SDS/PAGE separation step. Unlike the F1 and F4 forms, which were both myristoylated and palmitoylated, the F3 form was only palmitoylated. The monomeric, trimeric and tetrameric forms of PICE were all able to hydrolyse short chain fatty acids containing glycerides, as well as phorbol esters. The broad specificity of fatty acylated carboxylesterase is discussed in terms of its possible involvement in the metabolism of ester-containing xenobiotics and signal transduction.
Smialowski-Fléter
Sylvie
S
Institut Méditerranéen de Recherche en Nutrition, UMR-INRA, Faculté des Sciences et Techniques de St-Jérôme, Marseille, France.
Moulin
André
A
Perrier
Josette
J
Puigserver
Antoine
A
D000215:Acylation; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D020895:Enterocytes / Q000166:cytology / Q000201:enzymology; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000166:cytology / Q000201:enzymology*; D007526:Isoelectric Point; D008099:Liver / Q000166:cytology / Q000201:enzymology; D019814:Myristic Acid / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity; D013552:Swine; D014018:Tissue Distribution
D019814:Myristic Acid; D019308:Palmitic Acid; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase