Iron induces proliferation and morphogenesis in primmorphs from the marine sponge Suberites domuncula.
Dissociated cells from marine demosponges retain their proliferation capacity if they are allowed to form special aggregates, the primmorphs. On the basis of incorporation studies and septin gene expression, we show that Fe3+ ions are required for the proliferation of cells in primmorphs from Suberites domuncula. In parallel, Fe3+ induced the expression of ferritin and strongly stimulated the synthesis of spicules. This result is supported by the finding that the enzymatic activity of silicatein, converting organosilicon to silicic acid, depends on Fe3+. Moreover, the expression of a scavenger receptor molecule, possibly involved in the morphology of spicules, depends on the presence of Fe3+. We conclude that iron is an essential factor in proliferative and morphogenetic processes in primmorphs.
Krasko
Anatoli
A
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Mainz, Germany.
Schröder
Heinz C
HC
Batel
Renato
R
Grebenjuk
Vladislav A
VA
Steffen
Renate
R
Müller
Isabel M
IM
Müller
Werner E G
WE
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D005293:Ferritins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006639:Histidine / Q000737:chemistry; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D008565:Membrane Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011161:Porifera / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D011971:Receptors, Immunologic / Q000378:metabolism; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D051122:Scavenger Receptors, Class B; D017386:Sequence Homology, Amino Acid; D017640:Silicates / Q000378:metabolism
D005656:Fungal Proteins; D008565:Membrane Proteins; C410684:PCD1 protein, Pyrenopeziza brassicae; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D011994:Recombinant Proteins; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; D017640:Silicates; C112571:silicatein alpha; D006639:Histidine; D004247:DNA; D005293:Ferritins; D007501:Iron; D002403:Cathepsins