[Zymographic analysis of matrix metalloproteinases in mouse carcinoma cell lines with different lymphatic metastasis potential].
OBJECTIVE
To investigate the correlation between lymphatic metastasis and matrix metalloproteinases (MMPs) activity.


METHODS
Zymographic analysis was used to detect the difference of MMPs product and activities between high lymphatic metastatic cell line (HCa-F) and low lymphatic metastatic cell line (HCa-P), that were cultured in the medium including homogenate of lymph node or liver or spleen tissue.


RESULTS
HCa-F and HCa-P cells produced small quantities of MMP-9 in 1640 medium. After adding lymph node homogenate to the culture medium, F cells produced a large quantity of MMP-9 as well as active MMP-9 and MMP-2. P cells also produced MMP-9, active MMP-9 and MMP-2, but were much lower than that of F cells. Neither F cells nor P cells produced MMPs in the media with homogenate of liver or spleen added.


CONCLUSIONS
The metastatic potential of F cells is stronger than that of P cells may be due to F cell's ability to secrete MMPs is stronger than P cell's, therefore the production of MMPs of hepatocarcinoma cells is closely correlated to their metastatic potential.
Hou
L
L
Department of Pathology, Dalian Medical University, Dalian 116027, China.
Ling
M
M
Lu
S
S
Xin
Y
Y
Li
Y
Y
Wang
H
H
D000818:Animals; D008114:Liver Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D008207:Lymphatic Metastasis; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9