[The effects of escharectomy en masse during shock stage on the secretion of cytokines from macrophage in scalded rats].
OBJECTIVE
To explore the effects of the plasma from rats undergoing escharectomy en masse during shock stage on the secretion of TNFalpha, IL-6 and IL-8 by macrophage.


METHODS
Rats inflicted by 30% III degree scald were employed as the model. Sixty-three rats were randomly divided into escharectomy (E) and scald (C) groups. The plasma contents of TNFalpha, IL-6 and IL-8 in rats in two groups were determined. The plasma from rats in two groups was respectively co-cultured with rat peritoneal macrophages for 6 hours and the TNFalpha, IL-6 and IL-8 in the supernatants of the culture were assayed.


RESULTS
Plasma contents of TNFalpha, IL-6 and IL-8 were evidently increased in both E and C groups. But the contents of these cytokines in E group decrease and were much lower than those in C group at 12 postburn hour. The sera from two groups of rats could all stimulate the secretion of TNFalpha, IL-6 and IL -- 8 from peritoneal macrophages, but the serum from C group possessed stronger stimulating ability than that from E group.


CONCLUSIONS
Escharectomy en masse during shock stage could weaken the ability of burn sera to induce the secretion of TNFalpha, IL-6 and IL-8 by macrophages. It was therefore beneficial to the prevention of postburn development of systemic inflammatory response syndrome.
Li
Z
Z
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
Huang
Y
Y
Yang
Z
Z
D000818:Animals; D002056:Burns / Q000097:blood / Q000276:immunology / Q000601:surgery*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012774:Shock, Traumatic / Q000276:immunology*
D016207:Cytokines