Autocrine production of epithelial cell-derived neutrophil attractant-78 induced by granulocyte colony-stimulating factor in neutrophils.
Whereas mobilization to inflammatory sites is an important function of neutrophils, it remains to be determined whether granulocyte colony-stimulating factor (G-CSF) stimulates the mobilization of neutrophils to the inflammatory sites. This study compared the expression of more than 9000 genes in neutrophils treated with and without G-CSF with the use of a DNA microarray system to determine the effects of G-CSF on the function of neutrophils. It was found that messenger RNA expression of epithelial cell-derived neutrophil attractant-78 (ENA-78), which has been reported to be a chemotactic factor for neutrophils, was induced by G-CSF in neutrophils. The study demonstrated that the supernatant of G-CSF-treated neutrophils induced the chemotaxis of neutrophils and that anti-ENA-78 antibody and anti-CXCR-2 antibody inhibited the chemotaxis. These data suggest that G-CSF may enhance the mobilization of neutrophils and consequently augment the accumulation of neutrophils in the inflammatory sites through the secretion of ENA-78.
Suzuki
Sachiko
S
Division of Cancer Pathobiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
Kobayashi
Masanobu
M
Chiba
Kouji
K
Horiuchi
Iori
I
Wang
Jingxin
J
Kondoh
Takeshi
T
Hashino
Satoshi
S
Tanaka
Junji
J
Hosokawa
Masuo
M
Asaka
Masahiro
M
D019898:Autocrine Communication / Q000187:drug effects*; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects
C486263:CXCL5 protein, human; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D017077:Culture Media, Conditioned; D016209:Interleukin-8; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor