Anti-idiotype antibodies abrogate the tissue deposition of anti-RNP human autoantibodies injected into neonatal BALB/c mice.
OBJECTIVE
The goals of the current work are: 1) to examine the epidermal deposition of anti-RNP IgG human autoantibodies in neonatal BALB/c mice; 2) to look for immunoregulatory effects of anti-idiotypes allowing one to inhibit the epidermal deposition of anti-RNP antibodies; and 3) to elicit antinuclear antibodies in adult BALB/c mice by internal images of anti-idiotypes.


METHODS
Anti-idiotype antibodies were produced with human anti-RNP IgG obtained by ion exchange chromatography; F(ab')2 fragments were recovered from pepsin digestion and were purified using Sephacryl S-300. F(ab')2 fragments were then used to immunize New Zealand rabbits.


RESULTS
The anti-RNP IgG recognized the 70 kDa protein and the A (31 kDa) and C (19 kDa) proteins, while the anti-idiotype antibody specifically recognized the light or heavy chain of the anti-RNP (Fab')2 fragments. Additionally, anti-idiotypes recognized the anti-RNP IgG from some sera, but not the IgG from other specificities or from normal IgG. When anti-RNP IgG was injected intraperitoneally into BALB/c mice it induced immune complex deposition in the epidermis and at the dermal-epidermal junction. Previous injection of anti-idiotype antibodies abrogated the anti-RNP IgG deposits. Vaccination with anti-idiotypes elicit antinuclear antibodies in adult BALB/c mice.


CONCLUSIONS
Anti-idiotype antibodies abrogate in vitro the antinuclear antibody deposition in neonatal BALB/c mice. Anti-idiotype antibodies elicit antinuclear antibodies in adult BALB/c mice.
Cabral-Arellano
Francisco
F
Center for experimental biology, Universidad Autonoma de Zacatecas, Guadalupe, Mexico.
Avalos-Díaz
Esperanza
E
Sánchez-Rodríguez
Sergio H
SH
Villalobos
Ricardo
R
Herrera-Esparza
Rafael
R
D000818:Animals; D000831:Animals, Newborn; D000974:Antibodies, Antinuclear / Q000008:administration & dosage / Q000276:immunology*; D001324:Autoantigens / Q000008:administration & dosage / Q000276:immunology*; D003872:Dermatitis / Q000276:immunology* / Q000517:prevention & control; D006801:Humans; D007105:Immune Complex Diseases / Q000276:immunology* / Q000517:prevention & control; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology*; D007130:Immunoglobulin Idiotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012867:Skin / Q000276:immunology / Q000473:pathology; D055517:snRNP Core Proteins
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D055517:snRNP Core Proteins