[p15 gene expression in acute lymphoblastic leukemia cell line Molt4 induced by arsenic trioxide].
OBJECTIVE
To explore the correlation between arsenic trioxide and gene methylation in an acute lymphoblastic cell line.


METHODS
Methylation of p15 gene in Molt4 cell line was detected by polymerase chain reaction (PCR) using methylation specific primer (MSP) and the expression of this gene after arsenic trioxide treatment was detected by reverse transcriptase PCR (RT-PCR). The cell cycle and cell growth curve were also observed by flow cytometry.


RESULTS
p15 gene failed to express in molt4 after methylation. The expression was recovered, cell growth was inhibited, and G(1) cell cycle arrest was observed, when the cells exposed to arsenic.


CONCLUSIONS
Arsenic trioxide could activate the expression of p15 gene and reverse cell cycle negative regulation.
Jin
H
H
Hematological Department, General Hospital of Armed Police Forces, Beijing 100853, China.
Lou
F
F
Yu
L
L
D000208:Acute Disease; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D010087:Oxides / Q000494:pharmacology*
D001152:Arsenicals; D050762:Cyclin-Dependent Kinase Inhibitor p15; D010087:Oxides; D000077237:Arsenic Trioxide