Production of new unsaturated lipids during wood decay by ligninolytic basidiomycetes.
Lipids were analyzed by gas chromatography-mass spectrometry for a 7-week in vitro decay of eucalypt wood by four ligninolytic basidiomycetes. The sound wood contained up to 75 mg of lipophilic compounds per 100 g of wood. Hydrolysis of sterol esters, which represented 38% of total wood lipids, occurred during the fungal decay. The initial increase of linoleic and other free unsaturated fatty acids paralleled the decrease of sterol esters. Moreover, new lipid compounds were found at advanced stages of wood decay that were identified from their mass spectra as unsaturated dicarboxylic acids consisting of a long aliphatic chain attached to the C-3 position of itaconic acid. These dicarboxylic acids were especially abundant in the wood treated with Ceriporiopsis subvermispora (up to 24 mg per 100 g of wood) but also were produced by Phlebia radiata, Pleurotus pulmonarius, and Bjerkandera adusta. We hypothesize that three main alkylitaconic acids (tetradecylitaconic, cis-7-hexadecenylitaconic, and hexadecylitaconic acids) are synthesized by fungi in condensation reactions involving palmitic, oleic, and stearic acids. We suggest that both wood unsaturated fatty acids (present in free form or released from esters during natural decay) and unsaturated metabolites synthesized by fungi could serve as a source for peroxidizable lipids in lignin degradation by white rot basidiomycetes.
Gutiérrez
Ana
A
Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, E-41080 Seville, Spain.
del Río
José C
JC
Martínez-Iñigo
María J
MJ
Martínez
María J
MJ
Martínez
Angel T
AT
D001487:Basidiomycota / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005052:Eucalyptus / Q000382:microbiology*; D050356:Lipid Metabolism; D014934:Wood