Molecular aspects of photodynamic therapy: low energy pre-sensitization of hypericin-loaded human endometrial carcinoma cells enhances photo-tolerance, alters gene expression and affects the cell cycle.
Photosensitization of HEC1-B cells with a low concentration of hypericin and doses of light below 10 J/cm(2) caused cell death (apoptosis occurred mainly at doses between 2 and 5 J/cm(2), whereas necrosis prevailed above 6 J/cm(2)). However, pre-exposure of cells to innocuous irradiation (2 J/cm(2)) and successive challenge with a light dose that normally induced apoptosis (5 J/cm(2)) altered the expression of the proteins involved in the regulation of apoptosis, stress response and cell cycle. This change resulted in a significant increase in cell photo-tolerance.
Varriale
Linda
L
Dipartimento di Biologia e Patologia Cellulare e Molecolare 'L. Califano' and CEOS/CNR, Universitá di Napoli Federico II, Via S. Pansini 5, 80131, Naples, Italy.
Coppola
Eva
E
Quarto
Maria
M
Veneziani
Bianca Maria
BM
Palumbo
Giuseppe
G
D000873:Anthracenes; D017209:Apoptosis; D001692:Biological Transport; D002277:Carcinoma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D009336:Necrosis; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D010778:Photochemotherapy; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D011838:Radiation-Sensitizing Agents / Q000378:metabolism / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
D000873:Anthracenes; C494811:BCL2L1 protein, human; D018840:HSP70 Heat-Shock Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011838:Radiation-Sensitizing Agents; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D010569:Perylene; C004965:hypericin