[Genetic aspects of species structure of the compost worm Eisenia foetida (Sav.) (Oligochaeta, Lumbricidae)].
Distribution of frequencies alleles of polymorphous loci of peroxidase (Pox), leucineaminopeptidase (Lap), phosphoglucomutase (Pgm) and octanoldehydrogenase (Odh) were studied by electrophoresis in polyacrylamide gel in 22 local samples of Esenia foetida in Russia (European part), Ukraine, Kazakhstan and Kirghizia. The samples form two spatial groups--"northern" and "southern", distinguished by set of alleles in every studied locus. The "northern" groups is formed by local populations of European Russia from Murmansk region on the north to Smolensk region on the south, and also by cultivated population of selection line "red California hybrid". The "southern" group is formed by local populations on the territory of Russia from middle Volga to the North Caucasus, Ukraine, Kazakhstan, Kirghizia, cultivated populations from Kirghizia and Portugal. High degree of genetic difference between samples and independence of alleles frequencies distribution from geographical location and habitat allows to consider almost all studied groups as separate populations. Statistical processing of Nei genetic distances (Nei, 1972) revealed reliable differences between averages of within- and intergroup distances. Besides, discrete differences between intervals of significance of genetic distances were revealed. The results indicate that on the studied territory E. foetida has hierarchical two level structure. The first level is formed by local populations differed by frequency of the same alleles. The second level is formed by local populations, united into spatial groups, that are qualitatively distinguished by the set of alleles in the same loci.
Bolotetskiĭ
N M
NM
Laboratory of Animal Development, Biological Faculty, M.V. Lomonosov Moscow University, Vorob'evy Gory, Moscow 119899, Russia.
Kodolova
O P
OP
D000429:Alcohol Oxidoreductases / Q000235:genetics; D000483:Alleles; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005828:Genetics, Population; D007623:Kazakhstan; D007707:Kyrgyzstan; D007931:Leucyl Aminopeptidase / Q000235:genetics; D009835:Oligochaeta / Q000201:enzymology / Q000235:genetics*; D009195:Peroxidase / Q000235:genetics; D010733:Phosphoglucomutase / Q000235:genetics; D011110:Polymorphism, Genetic; D012426:Russia; D013045:Species Specificity; D014455:Ukraine
D000429:Alcohol Oxidoreductases; C021902:octanol dehydrogenase; D009195:Peroxidase; D007931:Leucyl Aminopeptidase; D010733:Phosphoglucomutase