Crystallization and preliminary X-ray diffraction studies of human angiostatin.
Angiostatin is an inhibitor of angiogenesis, the process by which new blood vessels are formed from pre-existing ones. The fact that tumor growth and metastasis dissemination are angiogenesis-dependent processes has awakened interest in angiogenesis inhibitors as anticancer treatment drugs. Angiostatin, a potent angiogenesis inhibitor, is currently in phase I clinical trials. Human angiostatin containing the first three kringle domains of plasminogen (K1-3) has been crystallized and high-resolution data were collected. The crystals belong to the P4(1)2(1)2 space group, with unit-cell parameters a = b = 56.94, c = 192.97 A. A data set to a resolution of 1.75 A with an overall R(merge) and I/sigma(I) of 7% and 19.5, respectively, was obtained.
Abad
Marta C
MC
Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Geiger
James
J
D043168:Angiostatins; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics; D010958:Plasminogen / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation
D010446:Peptide Fragments; D043168:Angiostatins; D010958:Plasminogen