[Detection of hypermethylation of p15 gene in patients with acute leukemia by polymerase chain reaction].
OBJECTIVE
To explore the role of hypermethylation of p15 gene in the pathogenesis of acute leukemias.


METHODS
DNA from 36 cases of acute leukemia were investigated using restriction enzyme digestion combined with polymerase chain reaction (PCR) technique.


RESULTS
Hypermethylations of p15 gene were found in 22 (61.1%) patients, including 17 cases of acute non-lymphoblastic leukemia (ANLL) and 5 of acute lymphoblastic leukemia (ALL). ANLL patients exhibited p15 gene hypermethylations had higher WBC in peripheral blood (P < 0.05) and higher percentages of leukemia cells in bone marrow (P < 0.01).


CONCLUSIONS
Inactivation of p15 gene due to hypermethylation may be involved in the pathogenesis of some acute leukemias.
Chen
F
F
Department of Hematology, the Second Affiliated Hospital, Hubei Medical University, Wuhan 430071, China.
Zeng
X
X
D000328:Adult; D000368:Aged; D018797:Cell Cycle Proteins / Q000235:genetics*; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins
C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D025521:Tumor Suppressor Proteins