The influence of deferiprone (L1) and deferoxamine on iron and essential element tissue level and parameters of oxidative status in dietary iron-loaded mice.
The seven week feeding of a diet enriched with 0.5% TMH-ferrocene to male mice was used in this study to produce an iron-overload model in experimental animals for evaluating the effect of deferoxamine (DFO) and deferiprone (L1) on tissue-stored iron, induced lipid peroxidation (LP) and parameters of oxidative status. The iron concentration in the liver reached 600% of the level in control animals. The administration of seven doses of deferoxamine (DFO) i.p. and deferiprone (L1) p.o. (0.72 mmol/kg b.w., every 48 h) during 9th and 10th week significantly decreased the liver, kidneys and heart iron level in both iron-loaded and control mice. The DFO and L1 treatment also equally attenuated lipid peroxidation and increased the GSH level in the liver of iron loaded mice. The glutathione peroxidase (GSH-Px) activity and catalase activity were not affected by iron loading, however, both DFO and L1 caused a decrease of GSH-Px activity.
Eybl
Vladislav
V
Department of Pharmacology and Toxicology, Charles University Faculty of Medicine in Pilsen, Karlovarská 48, 301 66 Pilsen, Czech Republic. vladislav.eybl@lfp.cuni.cz
Kotyzová
Dana
D
Kolek
Metodìj
M
Koutenský
Jaroslav
J
Nielsen
Peter
P
D000818:Animals; D002374:Catalase / Q000378:metabolism; D000077543:Deferiprone; D003676:Deferoxamine / Q000009:adverse effects / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007502:Iron Chelating Agents / Q000009:adverse effects / Q000494:pharmacology*; D019190:Iron Overload / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D019266:Iron, Dietary / Q000008:administration & dosage / Q000378:metabolism; D008054:Lipid Peroxides / Q000096:biosynthesis / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D011728:Pyridones / Q000009:adverse effects / Q000494:pharmacology*
D007502:Iron Chelating Agents; D019266:Iron, Dietary; D008054:Lipid Peroxides; D011728:Pyridones; D000077543:Deferiprone; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione; D003676:Deferoxamine