[Clone of a novel liver cancer associated gene and analysis of the secondary structures of the predicted protein].
OBJECTIVE
To clone a novel liver cancer associated gene, and to explore the molecular basis of liver cancer genesis.


METHODS
Using mRNA differential display polymerase chain reaction (DDPCR) and screening the human placenta cDNA library, we got a full-length cDNA of the gene. We prepared and purified the GST fusion protein and the special polyclonal antibody, engaged in the Western blot and immunohistochemical staining analysis, and analyzed the second structures and predicted the function of the protein by the computer soft.


RESULTS
We have got a full-length cDNA of the liver cancer associated gene and identified that the full-length cDNA of the gene could be expressed in 293 eukaryocytes by Western blot assay. We localized the target protein in cytoplasm using the immunohistochemical staining methods, and found two SH3 binding domains and several protein kinase phosphorylation sites by analyzing the second structures.


CONCLUSIONS
We have got a novel full-length cDNA of human liver cancer associated gene.
Wang
Zhengxu
Z
Department of General Surgery of Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, China.
Hu
Guifang
G
Wang
Hongyang
H
Wu
Mengchao
M
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D002460:Cell Line; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D004273:DNA, Neoplasm; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D009687:Nuclear Proteins; D010766:Phosphorylation; D016133:Polymerase Chain Reaction / Q000379:methods; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D015534:Trans-Activators; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011506:Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C456659:YY1AP1 protein, human