Characterization of a monomeric disintegrin, ocellatusin, present in the venom of the Nigerian carpet viper, Echis ocellatus.
Ocellatusin is a new RGD-containing short monomeric disintegrin. It is a better inhibitor of alpha(5)beta(1) integrin and a more potent inducer of the expression of a ligand-induced binding site epitope on beta(1) integrin subunit than echistatin. In further contrast to echistatin, ocellatusin has a direct chemotactic stimulus on human neutrophils in vitro. The distinct effects of these two close evolutionarily related disintegrins might be explained by the presence of methionine-22 and histidine-29 in the RGD loop of ocellatusin, which are arginine and aspartic acid, respectively, in echistatin. These mutations may modulate the conformation and/or recognition properties of the integrin-binding loop of ocellatusin.
Smith
J Bryan
JB
Department of Pharmacology, Sol Sherry Thrombosis Research Center, Temple University, School of Medicine, Philadelphia, PA 19140, USA.
Theakston
R David G
RD
Coelho
Ana Lucia J
AL
Barja-Fidalgo
Christina
C
Calvete
Juan J
JJ
Marcinkiewicz
Cezary
C
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D019483:Disintegrins / Q000302:isolation & purification* / Q000494:pharmacology; D000939:Epitopes; D006801:Humans; D020014:K562 Cells; D008024:Ligands; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects; D009842:Oligopeptides; D017469:Receptors, Fibronectin / Q000037:antagonists & inhibitors; D017386:Sequence Homology, Amino Acid; D014757:Viper Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D017819:Viperidae
D019483:Disintegrins; D000939:Epitopes; D008024:Ligands; D009842:Oligopeptides; D017469:Receptors, Fibronectin; D014757:Viper Venoms; C450420:ocellatusin; C047981:arginyl-glycyl-aspartic acid