Results of human papillomavirus DNA testing with the hybrid capture 2 assay are reproducible.
Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.
Castle
Philip E
PE
Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, USA. pc95p@nih.gov
Lorincz
Attila T
AT
Mielzynska-Lohnas
Iwona
I
Scott
David R
DR
Glass
Andrew G
AG
Sherman
Mark E
ME
Schussler
John E
JE
Schiffman
Mark
M
D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D015203:Reproducibility of Results; D019562:Viral Load
D004279:DNA, Viral