Extracellular serine protease neuropsin (KLK8) modulates neurite outgrowth and fasciculation of mouse hippocampal neurons in culture.
A serine protease neuropsin expressed in the hippocampus of adult brain has been implicated in synaptic plasticity. We report here that endogenous neuropsin was localized extracellularly in neuronal cell bodies and their neurites in mouse hippocampal cultures. Furthermore, we found that, in cultured mouse hippocampal neurons, recombinant neuropsin enhanced neurite projection from soma after 14 h of culture and neuronal aggregation with neurite fascicles at 48 h. This suggests that neuropsin is involved in neurite outgrowth and fasciculation during the development of the nervous system.
Oka
Takuya
T
Division of Structural Cell Biology, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara, Japan.
Akisada
Morito
M
Okabe
Akihito
A
Sakurai
Katsutoshi
K
Shiosaka
Sadao
S
Kato
Keiko
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects / Q000201:enzymology*; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D007150:Immunohistochemistry; D007610:Kallikreins; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000378:metabolism* / Q000494:pharmacology
D008869:Microtubule-Associated Proteins; D011994:Recombinant Proteins; C094799:KLK8 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases