[Regulation of c-myc expression in leukemic cell line Meg-01 by the death domain of Fas gene].
OBJECTIVE
To investigate the mechanism of disordered proliferation of leukemic cells and the proliferation inhibition by the Fas death domain (FASDD) in human leukemia cell line Meg-01.


METHODS
The chimerical receptors (Fas/190, Fas/130) were constructed by Fas cytoplasmic domain with the leukemic inhibitory factor (LIF) receptor subunits gp190 and gp130, another chimerical receptor was constructed by replacing the gp130 in Fas/130 with Fas death domain (FAS/130f). The chimerical receptors were separately expressed on the membrane of Meg-01, and activated with anti-Fas antibodies to induce the oligomerics of the cytoplasmic regions (190cyt-190cyt-190cyt, 130cyt-130cyt-130cyt or FASDD-FASDD-FASDD) for initiating the intracellular signal transduction. c-myc expression level was assayed by means of immunoblotting and immunobiochemistry.


RESULTS
Increased c-myc expression and cell proliferation were observed in the group of Fas/130 as compared with the other two groups.


CONCLUSIONS
The cytoplasmic domain of gp130 might involve the induction of c-myc expression and the cell proliferation of Meg-01 cell. The Fas death domain may be a medium in the apoptosis induction against the effect of gp130.
Liu
H
H
Histology and Embryology Department, Second Military Medical University, Shanghai 200433, China.
Shan
J
J
Tang
S
S
Liu
S
S
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050822:Cytokine Receptor gp130 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D053222:Fas Ligand Protein / Q000378:metabolism; D053200:Fas-Associated Death Domain Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D053606:Receptors, OSM-LIF / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D053222:Fas Ligand Protein; D053200:Fas-Associated Death Domain Protein; D016271:Proto-Oncogene Proteins c-myc; D053606:Receptors, OSM-LIF; D050822:Cytokine Receptor gp130