title: Replicon typing of F18 fimbriae encoding plasmids of enterotoxigenic and verotoxigenic Escherichia coli strains from porcine postweaning diarrhoea and oedema disease.
abstract: The presence of fimbrial adhesin F18 is frequently found in enterotoxigenic Escherichia coli (ETEC) and verotoxigenic E. coli (VTEC) strains responsible for diarrhoea and oedema disease of weaned pigs. The F18 adhesin occurs in two antigenic variants: F18ab is characteristic of VTEC while F18ac is more typical for ETEC. F18 encoding plasmids of 17 phenotypically characterized porcine E. coli isolates (10 ETEC, 6 VTEC and 1 ETEC/VTEC) were tested with a DNA probe for F18 fimbrial adhesin and with replicon probes for the RepFIa, RepFIb and for the RepFIc family of basic replicons. In all the cases, the F18 probe hybridized to only one plasmid band of size higher than 42MDa. All F18 plasmids were determined to be unireplicon plasmids belonging to the RepFIc replicon family of the F incompatibility complex. There was no difference between F18ac plasmids of ETEC and F18ab plasmids of VTEC strains in terms of replicon type or subtype. However, the size of F18ab plasmids of the VTEC strains varied between 42 and 98MDa, in contrast to F18ac plasmids of ETEC strains (constantly approximately 98MDa).
authors.affiliation: Veterinary Medical Research Institute of Hungarian Academy of Sciences, H-1143 Budapest, Hungária krt. 21, P.O. Box 18, H-1581, Budapest, Hungary.
authors.forename: P Zs
authors.initials: PZ
authors.lastname: Fekete
authors.forename: J
authors.initials: J
authors.lastname: Gerardin
authors.forename: E
authors.initials: E
authors.lastname: Jacquemin
authors.forename: J G
authors.initials: JG
authors.lastname: Mainil
authors.forename: B
authors.initials: B
authors.lastname: Nagy
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004488:Edema Disease of Swine / Q000382:microbiology*; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000235:genetics; D006824:Hybridization, Genetic; D010957:Plasmids / Q000145:classification; D012093:Replicon; D022603:Shiga Toxins / Q000235:genetics; D013552:Swine; D014886:Weaning
chemical_list: D018830:Adhesins, Escherichia coli; D004269:DNA, Bacterial; D004768:Enterotoxins; D022603:Shiga Toxins