Species-level identification of orthopoxviruses with an oligonucleotide microchip.
A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedure takes 6 h and does not require any sophisticated equipment (a portable fluorescence reader can be used).
Lapa
Sergey
S
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Mikheev
Maxim
M
Shchelkunov
Sergei
S
Mikhailovich
Vladimir
V
Sobolev
Alexander
A
Blinov
Vladimir
V
Babkin
Igor
I
Guskov
Alexander
A
Sokunova
Elena
E
Zasedatelev
Alexander
A
Sandakhchiev
Lev
L
Mirzabekov
Andrei
A
D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D018155:Orthopoxvirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
D015342:DNA Probes; D018124:Receptors, Tumor Necrosis Factor; D014764:Viral Proteins; C089458:crmB protein, Orthopoxvirus