Protooncogene Bcl-2 induces apoptosis in several cell lines.
Using a recombinant vaccinia virus expressing protooncogene Bcl-2, we demonstrate opposite effects of the expressed Bcl-2 in two cell lines: apoptosis induction in BSC-40 cells and apoptosis prevention in HeLa G cells. The apparent molecular weight of the expressed Bcl-2, its amounts and its effects on the mitochondrial membrane potential are comparable in both cell lines, suggesting that the consequences of Bcl-2 expression depend on the cellular environment. To further support these findings we demonstrate the pro-apoptotic effect of the expressed Bcl-2 in several other cell lines.
Kalbácová
M
M
Department of Pathological Physiology, 1st Medical Faculty, Charles University, Prague, Czech Republic.
Vrbacký
M
M
Humlová
Z
Z
Melková
Z
Z
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D002460:Cell Line / Q000166:cytology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000166:cytology; D017930:Genes, Reporter; D019254:Genes, bcl-2; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology; D019169:Jurkat Cells / Q000166:cytology; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013045:Species Specificity; D014162:Transfection
D019253:Proto-Oncogene Proteins c-bcl-2; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase; D020169:Caspases