[8-chloroadenosine inhibits the growth factor-induced proliferation of retinal pigment epithelial cells in vitro].
OBJECTIVE
To study the inhibitory effect of 8-chloroadenosine (8-Cl-A) on growth factor-induced proliferation of retinal pigment epithelial (RPE) cells in vitro.


METHODS
Primary culture and subculture of bovine RPE cells were established in vitro first and then tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), basic fibroblast growth factor (bFGF) plus different concentrations of 8-Cl-A (2-16 micromol/L) were added to the cells of third passage respectively. DNA synthesis in RPE cells was measured with (3)H-TdR incorporation.


RESULTS
Cells treated with TNF-alpha, IL-1beta, bFGF had a higher (3)H-TdR uptake than the basal. The increase was abolished by 8-Cl-A in a dose dependent manner (P < 0.05). At TNF-alpha, IL-1beta, bFGF with 8-Cl-A group, CPM was similar to basal at 16, 8, 2 micromol/L respectively (P > 0.05).


CONCLUSIONS
8-Cl-A can inhibit the three growth factor-induced proliferation of RPE cells.
Yang
P
P
Department of Ophthalmology, The Third Hospital, Beijing Medical University, Beijing 100083.
Zhang
H
H
D015762:2-Chloroadenosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2; D015762:2-Chloroadenosine; D004247:DNA; C075440:8-chloroadenosine