[The regulating effects of dopamine receptors on the level of adenosine 3, 5-cyclic monophosphate in bovine trabecular cells in vitro].
OBJECTIVE
To assay the regulating effects of dopaminergic agonists and antagonists on adenosine 3, 5-cyclic monophosphate (cAMP) level in bovine trabecular cells (BTC) in vitro.


METHODS
H(3)-cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC.


RESULTS
Dopamine, a dopamine receptor agonist, and fenoldopam, a selective DA(1) receptor agonist, caused a fold increase of cAMP in cultured BTC in vitro. These effects were blocked by halporide, a non-selective dopamine antagonist, and SCH23309, a selective DA(1) antagonist, but not blocked by phenoxybenzamin, an alpha-adrenergic antagonist, and timolol, a beta-adrenergic antagonist.


CONCLUSIONS
Bovine trabecular cells contain not only beta-receptors but also DA(1) receptors.
Lu
Y
Y
Department of Ophthalmology, Beijing Xuanwu Hospital, Beijing 100053.
Li
M
M
D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis*; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D007429:Intraocular Pressure / Q000187:drug effects; D011943:Receptors, Adrenergic, beta / Q000032:analysis; D017447:Receptors, Dopamine D1 / Q000032:analysis / Q000502:physiology*; D014129:Trabecular Meshwork / Q000737:chemistry* / Q000166:cytology
D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D011943:Receptors, Adrenergic, beta; D017447:Receptors, Dopamine D1; D000242:Cyclic AMP