Transfer at the blastocyst stage of embryos derived from testicular round spermatid injection.
BACKGROUND
Intracytoplasmic injection of testicular round spermatids has been suggested as a salvage treatment in couples when testicular sperm extraction does not yield any mature sperm. However, the success of the procedure is debatable, and controversy surrounds issues such as the presence and (if present) identification of spermatids in testicular tissue. Progression rate to the blastocyst stage of spermatid-derived embryos appears to be low.


METHODS
In this study, we investigated the feasibility and outcome of blastocyst stage embryo transfer after round spermatid injection (ROSI). ROSI was undertaken in 58 couples who did not yield mature or elongated sperm to testicular sperm extraction.


RESULTS
The incidence of blastocyst formation from two pronuclear oocytes was 7.6%. A total of 16 blastocysts were transferred in 12 patients (20.7%). None of the patients conceived.


CONCLUSIONS
The results of this study indicate that the blastocyst stage is reached by only very few ROSI-derived embryos and these embryos do not implant.
Urman
Bulent
B
The Assisted Reproduction Unit, American Hospital of Istanbul, Guzelbahce sok. No:20, Nisantasi 80200, Istanbul, Turkey. burman@superonline.com
Alatas
Cengiz
C
Aksoy
Senai
S
Mercan
Ramazan
R
Nuhoglu
Alp
A
Mumcu
Alper
A
Isiklar
Aycan
A
Balaban
Basak
B
D048429:Cell Size; D004624:Embryo Transfer; D005260:Female; D005306:Fertilization; D006801:Humans; D008297:Male; D020554:Sperm Injections, Intracytoplasmic; D013087:Spermatids / Q000502:physiology*; D013737:Testis / Q000166:cytology*; D017211:Treatment Failure