[The effects of ecdysterone on the apoptosis and necrosis of cultured HUVEC induced by endotoxin].
OBJECTIVE
To study the protective effects of ecdysterone (EDS) on the apoptosis and necrosis of cultured HUVEC induced by endotoxin.


METHODS
HUVEC was cultured in vitro. Apoptosis and necrosis of HUVEC were induced by LPS, and EDS was added in test specimen. Apoptosis was determined by means of flow cytometry and fluorescent microscopy.


RESULTS
There was relatively less number of apoptosis and necrosis of in vitro cultured normal HUVEC than that after LPS stimulation, whereas apoptosis dominated over necrosis. After EDS was added to the culture of HUVEC, cell apoptosis and necrosis decreased simultaneously.


CONCLUSIONS
EDS might exert protective effects on cultured HUVEC against apoptosis and necrosis induced by LPS.
Shi
F
F
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038 P.R. China.
Zhang
J
J
Zhang
M
M
D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004441:Ecdysterone / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D014471:Umbilical Veins / Q000166:cytology
D004731:Endotoxins; D008070:Lipopolysaccharides; D004441:Ecdysterone