title: Sequence analysis of the complete genome of an iridovirus isolated from the tiger frog.
abstract: We have isolated a tiger frog virus (TFV) from diseased tiger frogs, Rana tigrina rugulosa. The genome was a linear double-stranded DNA of 105,057 basepairs in length with a base composition of 55.01% G+C. About 105 open reading frames were identified with coding capacities for polypeptides ranging from 40 to 1294 amino acids. Computer-assisted analyses of the deduced amino acid sequences revealed that 39 of 105 putative gene products showed significant homology to functionally characterized proteins of other species in the GenBank/EMBL/DDBJ databases. These proteins included enzymes and structural proteins involved in virus replication, transcription, modification, and virus--host interaction. The deduced amino acid sequences of TFV gene products showed more than 90% identity to FV3, but a low degree of similarity among TFV, ISKNV, and LCDV-1. The results from this study indicated that TFV may belong to the genus Ranavirus of the family Iridoviridae.
authors.affiliation: State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, People's Republic of China. lsbrc05@zsu.edu.cn
authors.forename: Jian G
authors.initials: JG
authors.lastname: He
authors.forename: Ling
authors.initials: L
authors.lastname: Lü
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Hua H
authors.initials: HH
authors.lastname: He
authors.forename: Shao P
authors.initials: SP
authors.lastname: Weng
authors.forename: Xiao H
authors.initials: XH
authors.lastname: Wang
authors.forename: Song Y
authors.initials: SY
authors.lastname: Zhou
authors.forename: Qin X
authors.initials: QX
authors.lastname: Long
authors.forename: Xun Z
authors.initials: XZ
authors.lastname: Wang
authors.forename: Siu M
authors.initials: SM
authors.lastname: Chan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016679:Genome, Viral; D006801:Humans; D007497:Iridoviridae / Q000235:genetics; D017994:Iridovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011898:Ranidae / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins