[Immunohistochemical findings on liver tissue in patients with hepatitis C].
OBJECTIVE
To investigate the distributions and infer relations of HCV antigens in the liver tissues.


METHODS
Immunohistochemical techniques as polyclonal antibody to HCV, monoclonal antibody to HCV - NS3 and monoclonal antibody to HCV - NS5 were used to detect HCV antigens in 107 patients with hepatitis C.


RESULTS
HCV antigens in liver tissues were identified on these three kinds of antibodies. The positive rates were 38.3%, 28.0% and 52.3% respectively. The positive granules were located in the cytoplasma of liver cells. The distributions of positive cells were scattered, diffusive and clustered. No obvious anatomic association was observed between the expression of HCV - NS3 and inflammatory reaction. HCV - NS5 positive cells were more commonly seen around or in the flammatory focci.


CONCLUSIONS
The expressions of various components of HCV were demonstrated in different concentrations. The expressions of HCV - NS5 might be related to the pathogenesis of hepatitis C.
Chen
X
X
Taishan Medical College, Taian 271000, China.
Xuan
M
M
Yin
Y
Y
Li
L
L
Wei
Y
Y
Zhao
X
X
D000328:Adult; D000368:Aged; D005260:Female; D006526:Hepatitis C / Q000175:diagnosis / Q000276:immunology*; D018936:Hepatitis C Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000821:virology*; D008297:Male; D008875:Middle Aged; D017361:Viral Nonstructural Proteins / Q000032:analysis*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; D012324:RNA-Dependent RNA Polymerase
D018936:Hepatitis C Antigens; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase