title: Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea.
abstract: Mosquitoes of the Anopheles bancroftii group collected from Northern Australia and Papua New Guinea (PNG) were investigated for sequence variation within the ribosomal DNA ITS2. Wing fringe morphology originally used to identify members of this group was compared to genotypes identified by restriction fragment length polymorphism analysis (RFLP) and heteroduplex analysis (HDA) of the rDNA ITS2. Members of this group separated into four RFLP genotypes (A, B, C and D) with some genotypes displaying wing fringe polymorphisms. Heteroduplex analysis of the ITS2 within and between populations identified genotype A as containing two geographically separate ITS2 sequences: A1 from the Northern Territory of Australia and A2 from Queensland and the Western Province of PNG. Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome.
authors.affiliation: Department of Cell and Molecular Biology, University of Technology Sydney, Australia. nigel.beebe@uts.edu.au
authors.forename: N W
authors.initials: NW
authors.lastname: Beebe
authors.forename: J
authors.initials: J
authors.lastname: Maung
authors.forename: A F
authors.initials: AF
authors.lastname: van den Hurk
authors.forename: J T
authors.initials: JT
authors.lastname: Ellis
authors.forename: R D
authors.initials: RD
authors.lastname: Cooper
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D001315:Australia; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010219:Papua New Guinea; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014921:Wings, Animal / Q000033:anatomy & histology
chemical_list: D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D009692:Nucleic Acid Heteroduplexes