Regeneration of neomucosa using cell-seeded collagen-GAG matrices in athymic mice.
Tissue engineering of oral mucosa could allow improved reconstructive options for intraoral tissue defects. Porous collagen-glycosaminoglycan (CG) matrices coated with a silicone elastomer were seeded by centrifugation with cultured human oral mucosal epithelial cells (strain OKG4 gingival keratinocytes) at a density of 250,000 cells per square centimeter. Full-thickness dorsal wounds (1.5 x 1.5 cm) were created surgically on each athymic mouse and were treated with either a seeded matrix or an unseeded matrix, or they were left open as a control. The CG matrices reduced the degree of wound contraction at day 14 compared with open wounds. The epithelial thickness of seeded matrices at day 14 was significantly greater (p = 0.0001) than that of unseeded matrices. Seeded matrices had more rapid degradation at 14 days compared with unseeded matrices. Human oral mucosal cells seeded into CG matrices contribute to form a stratified and differentiated epithelial layer during revascularization, cellular infiltration, and degradation of the CG matrix.
Butler
Charles E
CE
Department of Plastic Surgery, University of Texas, M.D. Anderson Cancer Center, Houston, TX, USA.
Navarro
Fernando A
FA
Park
Christine S
CS
Orgill
Dennis P
DP
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D024042:Collagen Type I; D006025:Glycosaminoglycans; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000166:cytology / Q000637:transplantation*; D012038:Regeneration; D023822:Tissue Engineering; D014945:Wound Healing
D024042:Collagen Type I; D006025:Glycosaminoglycans