[The determination of neuron-specific enolase of serum in the diagnosis and supervision of retinoblastoma].
OBJECTIVE
To investigate the value of determining quantity of neuron-specific enolase (NSE) in the diagnosis and supervision in patients with retinoblastoma (Rb).


METHODS
We used rapid electrophoretic and the fluorometer method in the 35 serum specimens from 35 patients with Rb during surgery, after enucleation, respectively, at 3, 6, 12, >or= 18 months and 31 serum specimens from 31 control cases to determine the quantity of NSE and enolase isoenzymes.


RESULTS
There were 207 +/- 65.9 u/L of all isoenzymes and 98.4 +/- 40.3 u/L of NSE from 35 patients with Rb. In comparison with the levels of control cases (respectively, 140.7 +/- 46.0 U//L of all isoenzymes, 41 +/- 10.9 u/L of NSE), both serum levels in patients with Rb increased obviously, the difference being significant. At 6, 12, and >or= 18 months after surgery, the determinations of all isoenzymes and NSE from 35 patients with Rb decreased step by step, the difference being significant in comparison with the values before the surgery. After surgery >or= 18 months, the levels of NSE and all enolase decreased to that of NSE and all enolase from control cases.


CONCLUSIONS
The determination of enolase level, especially NSE of serum, is valuable for the diagnosis of patients with Rb.
Wu
Z
Z
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060.
Mao
Y
Y
Yang
H
H
Pan
S
S
Chen
Z
Z
D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood*; D019572:Retinal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000601:surgery; D012175:Retinoblastoma / Q000097:blood / Q000175:diagnosis* / Q000601:surgery
D007527:Isoenzymes; D010751:Phosphopyruvate Hydratase