Evaluation of the Abbott LCx Mycobacterium tuberculosis assay for direct detection of Mycobacterium bovis in bovine tissue samples.
The commercial LCx amplification assay, usually employed to detect the Mycobacterium tuberculosis complex in respiratory specimens, was evaluated by comparing the results it gave with those obtained using Lowenstein-Jensen solid medium and pathological findings in 55 lymph nodes from cattle with positive and 10 lymph nodes from cattle with negative skin tests for tuberculosis. Fifty-three cultures (51 and 2, respectively) were positive for M. bovis, while the results for the LCx assay and the histological method were positive in 48 (45, 3) and 24 (20, 4) samples, respectively. None of the samples from cattle from certified tuberculosis-free herds were positive by any of the procedures. The results obtained with the LCx assay, compared with the culture procedure, regarded as the gold standard among the diagnostic techniques, gave a specificity of 91.6% and sensitivity of 90.5%. Although the sensitivity of LCx was suboptimal, DNA of M. bovis was detected in 81.8% of the skin test-positive animals. Amplification techniques could provide a rapid and reasonably reliable tool for detecting bovine tuberculosis.
Valente
C
C
Infectious Diseases, School of Veterinary Medicine, University of Perugia, Italy. valente@unipg.it
Cuteri
V
V
Ausili
E
E
Piersimoni
C
C
D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D022521:Ligase Chain Reaction; D008198:Lymph Nodes / Q000382:microbiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000382:microbiology*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic