[Mutation BRCA1 gene in 186 breast cancer patients].
OBJECTIVE
To analysis the mutation of BRCA1 gene in 186 breast cancer patients.


METHODS
Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and sequential were used in this analysis.


RESULTS
13/186 of patients showed mutation which comprised 7% of the total number of patients. In 8 patients, the mutation was observed in the intron splicing region and in 5 patients, the mutation was on the exon.


CONCLUSIONS
Mutation analysis of BRCA1 gene is helpful in the determination of developmental potential, early diagnosis and gene therapy for breast cancer.
Lai
C
C
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Jiang
Z
Z
Song
S
S
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D017422:Sequence Analysis, DNA