Identification of genes with higher expression in human uterine leiomyomas than in the corresponding myometrium.
We used a PCR-based subtraction method, representational difference analysis of cDNA (cDNA--RDA), to identify genes with a higher expression in leiomyomas in comparison with the corresponding myometrium during the proliferative phase of the menstrual cycle. Increased expression of the genes for pregnancy-associated plasma protein A (PAPPA), tomoregulin, cellular retinoid acid binding protein 1 (CRABP1), zinc finger protein 185 (ZFP 185) and latent transforming growth factor beta binding protein 2 (LTBP2) was demonstrated in individual leiomyoma samples compared with corresponding myometrium. Additionally, a specific positive immunostaining of LTBP2 was found in the smooth muscle cells of both leiomyomas and myometrium. These genes may be part of previously unidentified molecular mechanisms responsible for the selective growth advantage of leiomyomas compared with myometrium. This work expands our knowledge about the molecular nature of leiomyomas and provides novel candidate genes to further explore in relation to their function during leiomyoma growth.
Wu
Xuxia
X
Division for Reproductive Endocrinology, Department of Woman and Child Health, Karolinska Hospital L5:01, S-171 76 Stockholm, Sweden.
Blanck
Agneta
A
Norstedt
Gunnar
G
Sahlin
Lena
L
Flores-Morales
Amilcar
A
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D010450:Endopeptidases / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D060588:LIM Domain Proteins; D053779:Latent TGF-beta Binding Proteins; D007889:Leiomyoma / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D009215:Myometrium / Q000378:metabolism*; D009363:Neoplasm Proteins; D011257:Pregnancy Proteins / Q000235:genetics*; D011266:Pregnancy-Associated Plasma Protein-A; D012177:Retinol-Binding Proteins / Q000235:genetics*; D054840:Retinol-Binding Proteins, Cellular; D054839:Retinol-Binding Proteins, Plasma; D016212:Transforming Growth Factor beta; D014594:Uterine Neoplasms / Q000235:genetics*; D014599:Uterus; D016335:Zinc Fingers
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C509035:LTBP2 protein, human; D053779:Latent TGF-beta Binding Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011257:Pregnancy Proteins; C518456:RBP1 protein, human; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D054839:Retinol-Binding Proteins, Plasma; C423567:TMEFF2 protein, human; D016212:Transforming Growth Factor beta; C108627:ZNF185 protein, human; D010450:Endopeptidases; C430889:PAPPA2 protein, human; D011266:Pregnancy-Associated Plasma Protein-A