Macroporous poly(L-lactide) scaffold 1. Preparation of a macroporous scaffold by liquid--liquid phase separation of a PLLA--dioxane--water system.
A biodegradable poly(L-lactic acid) (PLLA) macroporous scaffold with a regular and highly interconnected structure in the size range from 50 to 150 mu m was fabricated from a PLLA--dioxane--water ternary system with the use of the thermally induced phase separation (TIPS) process. The phase diagram of PLLA with molecular weight above 200,000 was measured. It was found that a small change in the water content in the solvent caused a large shift in the cloud-point temperature. The porous morphology of the scaffold was closely related to the quenching route and formulation parameters, including polymer concentration, quenching temperature, aging time, and solvent composition of the ternary system. The porous morphology development in the scaffold was recorded as a function of aging time by scanning electronic microscopy (SEM). For systems with lower polymer concentrations (<4.5 wt%), polymer sedimentation occurred in the later stages of phase separation. A slight increase in the water content of the solvent mixture caused the sedimentation boundary to expand to higher polymer concentration. For systems with higher polymer concentrations (> or = 4.5 wt%), the development of phase separation was restricted by gelation that resulted from the crystallization of the PLLA chains. This gelation effect was greater at high polymer concentrations and low quenching temperatures. The macroporous expected scaffold could be optimized from the slow development of phase separation during the long coarsening process.
Hua
Feng Jun
FJ
Center for Advanced Functional Polymers, Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon, Kyungki 440-746, Korea.
Kim
Go Eun
GE
Lee
Jong Doo
JD
Son
Yong Keun
YK
Lee
Doo Sung
DS
D020341:Absorbable Implants / Q000592:standards*; D002152:Calorimetry, Differential Scanning; D004146:Dioxanes; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D012997:Solvents / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods; D014867:Water
D004146:Dioxanes; D011091:Polyesters; D012997:Solvents; D014867:Water; C033616:poly(lactide); C025223:1,4-dioxane