[Aminopeptidase inhibitor Bestatin induces HL-60 cell apoptosis through activating caspase 3].
OBJECTIVE
To study the variation and significance of caspase 3 activity in the process of amino-peptidase inhibitor--bestatin (BS) inducing human leukemic cell apoptosis.


METHODS
Cell apoptosis was evaluated by light microscopy, TUNEL labeling and flow cytometry (FCM). Caspase 3 activity was detected by colorimetry. The mitochondrial transmembrane potentials (DeltaPsi(m)) were detected by Rhodamine123 staining.


RESULTS
The apoptotic morphology, apoptotic peak on FCM and positive Annexin V(FITC) on cell membrane showed that BS could induce HL-60 cell apoptosis in a dose- and time-dependent manner. Caspase 3 activity was significantly higher in the apoptotic cells than in control cells. The apoptosis induced by BS was inhibited by AC-DEVD-CHO. The DeltaPsi(m) of cells treated with BS declined.


CONCLUSIONS
BS induces apoptosis of human acute leukemic cells through activation of caspase 3.
Lin
M
M
Department of Hematology, First Hospital of Medical Institute of Zhejiang University, Hangzhou 310003, China.
He
J
J
Cai
Z
Z
Qian
W
W
D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism*; D003124:Colorimetry; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D020287:In Situ Nick-End Labeling; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D000626:Aminopeptidases; D053148:Caspase 3; D007930:Leucine; C012211:ubenimex