title: [Detection of trisomy 8 with interphase fluorescence in situ hybridization in myelodysplastic syndromes].
abstract: OBJECTIVE
  To explore the value of interphase fluorescence in situ hybridization (FISH) in the detection of trisomy 8 in myelodysplastic syndromes (MDS).
  
  
  METHODS
  Conventional cytogenetics (CC) and interphase FISH using SpectrumGreen labelled chromosome 8 centromere specific probe were simultaneously carried out to detect trisomy 8 in 69 MDS and 6 normal individuals.
  
  
  RESULTS
  Two hundred interphase cells were counted and cells with three green hybridization spots > 3% was assigned. Eleven cases displayed trisomy 8 by CC and were confirmed in 10 by FISH. In 7 cases, the percentage of trisomy 8 cells was significantly lower by FISH than by CC. Seven cases displayed trisomy 8 by FISH in 58 cases who did not show trisomy 8 by CC. Of the 7 cases, two had 2 and 3 marker chromosomes respectively, 4 had normal karyotypes.
  
  
  CONCLUSIONS
  Interphase FISH was a useful method for the detection of trisomy 8 in MDS, especially in patients with normal karyotype or marker chromosome. It was a important complement to CC.
authors.affiliation: First Affiliated Hospital of Suzhou Medical College, Jiangsu Institute of Hematology, Suzhou 215006, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: Y
authors.initials: Y
authors.lastname: Xue
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: C
authors.initials: C
authors.lastname: Ruan
mesh_terms: D000328:Adult; D000368:Aged; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D014314:Trisomy