title: Single-cell viability assessment with a novel spectro-imaging system.
abstract: Single-cell viability assessment by means of plural dye probes require the spectral and temporal analysis of microscopic images of the test cells. To meet this requirement, we have developed a simple and compact spectro-imaging system using an image slicer and a grism. The image slicer was made of a bundle of 100 optical fibers. The field of view is divided into 10 x 10 sections. The spectral data of each section could be recorded every 5 s in the range from 400 to 800 nm at 5 nm resolution. The viability changes of yeast or tobacco single-cells were measured with this system. Using BY-2 cells, for example, the response to a chemical stress of saponin was measured by means of two fluorescent probes. The spectral-spatial-temporal data of fluorescein and DNA bound ethidium bromide provided us with useful information about the dynamic change of cell membrane permeability from which the cell viability was assessed.
authors.affiliation: Department of Biotechnology and Life Science, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16, Nakamachi, Koganei, Tokyo 184-8588, Japan. bio-func@cc.tuat.ac.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuoka
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kosai
authors.forename: Mikako
authors.initials: M
authors.lastname: Saito
authors.forename: Norihide
authors.initials: N
authors.lastname: Takeyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Suto
mesh_terms: D000666:Amphotericin B / Q000633:toxicity; D000935:Antifungal Agents / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003592:Cytophotometry / Q000295:instrumentation* / Q000379:methods*; D004996:Ethidium / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology; D012503:Saponins / Q000633:toxicity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D012503:Saponins; D000666:Amphotericin B; D004996:Ethidium