Aspirin-sensitive versus non-aspirin-sensitive nasal polyp patients: analysis of leukotrienes/Fas and Fas-ligand expression.
OBJECTIVE
The goal of the present study was to identify levels of leukotrienes released by leukocytes and nasal polyps from aspirin-sensitive patients (ASPs) and non-aspirin-sensitive patients (NASPs) after exposure to various concentrations of aspirin. Eosinophil expressions of apoptosis regulators were also compared.


METHODS
Leukotriene release from nasal polyps and leukocytes of ASPs and NASPs was measured after exposure to differing concentrations of aspirin. Eosinophils were analyzed for Fas and Fas ligand receptors using flow cytometry.


RESULTS
NASPs showed increased leukotriene release from nasal polyps on increased aspirin exposure. Polyps from ASPs did not show any change. ASPs showed greater leukotriene release from blood on increasing aspirin exposure compared with NASPs. Flow cytometric analysis showed eosinophils in the peripheral blood of ASPs have decreased Fas expression compared with those of NASPs.


CONCLUSIONS
This study showed differences in leukotriene release and eosinophil apoptosis between ASPs and NASPs.


CONCLUSIONS
These findings suggest a role of leukotrienes and eosinophils in aspirin sensitivity mechanisms in blood.
Ziroli
Nancy E
NE
Department of Otolaryngology-Head and Neck Surgery, Stritch School of Medicine, Loyola University Medical Center, Maywood, Illinois 60153, USA.
Na
Hanjo
H
Chow
James M
JM
Stankiewicz
James A
JA
Samter
Max
M
Young
M Rita I
MR
D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D017209:Apoptosis; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000276:immunology / Q000503:physiopathology; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D015289:Leukotrienes; D019014:fas Receptor; D001241:Aspirin