title: De novo production of (+)-aristolochene by sporulated surface cultures of Penicillium roqueforti.
abstract: The de novo production of the fungal metabolite, (+)-aristolochene by sporulated surface cultures of Penicillium roqueforti is reported for the first time. The biosynthesis of fungal volatiles by various sporulated surface cultures was monitored by solid phase micro-extraction (SPME). When comparing malt extract agar with sabouraud dextrose agar, the highest yield of the fungal metabolite (0.04 mg/ml of culture) was obtained with the latter medium. The biosynthesis of (+)-aristolochene showed a maximum during the fourth day after inoculation.
authors.affiliation: Department of Organic Chemistry, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. jan.demyttenaere@rug.ac.be
authors.forename: Jan C R
authors.initials: JC
authors.lastname: Demyttenaere
authors.forename: An
authors.initials: A
authors.lastname: Adams
authors.forename: Kristof
authors.initials: K
authors.lastname: Van Belleghem
authors.forename: Norbert
authors.initials: N
authors.lastname: De Kimpe
authors.forename: Wilfried A
authors.initials: WA
authors.lastname: König
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Tkachev
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques; D007700:Kinetics; D010407:Penicillium / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D013499:Surface Properties; D013997:Time Factors
chemical_list: D003470:Culture Media; D012717:Sesquiterpenes; C054277:aristolochene