[The induction of high-level expression of a novel VL-30 like gene during the chemical-inducing erythroid differentiation of murine erythroleukemia cells (MEL)].
OBJECTIVE
To study the gene expression during the chemical inducing erythroid differentiation of murine erythroleukemia cells (MEL).


METHODS
Differential display assay was used to analyze the gene expression before and after the induction differentiation of MEL cells by DMSO and hemin; and then, the flanking sequences of one of the cDNA fragment was amplified by modified rapid amplification of cDNA end (RACE) method, Northern blot analysis was adopted to characterize the expression of this gene.


RESULTS
The expression of a new transcript similar to VL-30 retrotransposon family increased significantly during the chemical inducing erythroid differentiation in MEL cells. The cloned cDNA is 1883bp, and it is highly homologous to the internal region of murine BVL-1 (1,955-4,000 nt). At least three transcripts in MEL cells were detected by Northern hybridization and all of them increased after the induction.


CONCLUSIONS
A new VL-30-like gene is identified for the first time in MEL cells during chemical inducing erythroid differentiation.
Chen
Y
Y
Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005, China.
Liu
D
D
Xue
S
S
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004121:Dimethyl Sulfoxide / Q000009:adverse effects; D015973:Gene Expression Regulation, Leukemic; D004915:Leukemia, Erythroblastic, Acute / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D051379:Mice; D008969:Molecular Sequence Data; D018626:Retroelements
D018626:Retroelements; D004121:Dimethyl Sulfoxide