Study on the supramolecular interaction of curcumin and beta-cyclodextrin by spectrophotometry and its analytical application.
The supramolecular interaction of curcumin and beta-cyclodextrin (beta-CD) has been studied by spectrophotometry. The mechanism of the inclusion was studied and discussed based on the variations of pK(a), absorption intensity, and infrared spectrograms. The results show that beta-CD reacts with curcumin to form a 2:1 host-guest complex with an apparent formation constant of 5.53 x 10(5) mol(-2) x L2. Based on the enhancement of the absorbance of curcumin produced through complex formation, a spectrophotometric method for the determination of curcumin in bulk aqueous solution in the presence of beta-CD was developed. The linear relationship between the absorbance and curcumin concentration was obtained in the range of 0-15 microg/mL, with a correlation coefficient (r) of 0.9991. The detection limit was 0.076 microg/mL. The proposed method was used to determine the curcumin in curry and mustard with satisfactory results.
Tang
Bo
B
Department of Chemistry, Shandong Normal University, Jinan 250014, People's Republic of China. tangb@sdnu.com
Ma
Li
L
Wang
Huai-you
HY
Zhang
Guo-ying
GY
D000097:Acetonitriles; D003474:Curcumin / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004347:Drug Interactions; D006863:Hydrogen-Ion Concentration; D012996:Solutions; D012997:Solvents; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics; D047392:beta-Cyclodextrins
D000097:Acetonitriles; D003505:Cyclodextrins; D012996:Solutions; D012997:Solvents; D047392:beta-Cyclodextrins; D003474:Curcumin; C031215:betadex; C032159:acetonitrile