title: HIV-1 infection of primary effusion lymphoma cell line triggers Kaposi's sarcoma-associated herpesvirus (KSHV) reactivation.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus etiologically linked to primary effusion lymphoma (PEL), to a subset of multicentric Castleman's disease and to Kaposi's sarcoma (KS), the most common neoplasm associated with AIDS. Among KSHV-infected individuals, the risk of KS is much higher in those with human immunodeficiency-1 (HIV-1) infection than among those with other types of immunosuppression, suggesting a direct action of HIV-1 on KSHV replication. We show in our report that BC-3 cells, a chronically KSHV-infected B-cell line of a PEL origin, are permissive to HIV-1, offering a new tool for studying the interactions between these 2 viruses. In these cells, HIV-1 infection leads to reactivation of latent KSHV genomes, as demonstrated by the expression of KSHV lytic viral mRNAs. Although recombinant HIV-1 gp120 fails to enhance herpesvirus expression, transient transfection of the HIV-1 trans-activator Tat suffices to reactivate latent KSHV. By showing that HIV-1 infection directly reactivates latent KSHV, our results suggest a direct role of HIV-1 in the onset of KS in coinfected individuals.
authors.affiliation: Policlinique de Dermatologie, Hôpital Saint-Louis, Paris, France. rastine.merat@chu.stlouis.fr
authors.forename: Rastine
authors.initials: R
authors.lastname: Merat
authors.forename: Ali
authors.initials: A
authors.lastname: Amara
authors.forename: Celeste
authors.initials: C
authors.lastname: Lebbe
authors.forename: Hugues
authors.initials: H
authors.lastname: de The
authors.forename: Patrice
authors.initials: P
authors.lastname: Morel
authors.forename: Ali
authors.initials: A
authors.lastname: Saib
mesh_terms: D001426:Bacterial Proteins; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D019288:Herpesvirus 8, Human / Q000254:growth & development*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000821:virology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D012514:Sarcoma, Kaposi / Q000821:virology*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000378:metabolism; D014775:Virus Activation; D014779:Virus Replication; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D015696:Gene Products, tat; D015699:HIV Envelope Protein gp120; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C406264:orf29 protein, Listeria monocytogenes; D054322:tat Gene Products, Human Immunodeficiency Virus