title: Abnormal "low grade" transformation zone: current diagnostic gold standard.
abstract: The aim of this work was to examine different methods of investigation in the diagnosis of the abnormal "low grade" transformation zone of the portio. Over a period of one year 41 patients subjected to colposcopic examination underwent exo-endocervical sampling for oncologic evaluation and for detection of viral and bacterial infections (HPV, HSV, adenovirus, mycoplasmas and chlamydia trachomatis), as well as portio biopsy. A 65.8% correlation was found between cytology and the HPV-DNA test results, while histology and the presence of the HPV virus agreed in 51.4% of cases. In those cases in which minimal histological alterations were found (koilocytosis) a high percentage of HPV negativity was found. In discordant negative cytologic tests that were however positive for HPV by PCR, the genotypes identified were always 6 and 11.
authors.affiliation: Institute of Gynecology and Obstetrics, University of Rome La Sapienza, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lukic
authors.forename: M
authors.initials: M
authors.lastname: Signore
authors.forename: F
authors.initials: F
authors.lastname: Nobili
authors.forename: A M
authors.initials: AM
authors.lastname: Degener
authors.forename: P L
authors.initials: PL
authors.lastname: Alò
authors.forename: M R
authors.initials: MR
authors.lastname: Giovagnoli
authors.forename: G
authors.initials: G
authors.lastname: Vetrano
authors.forename: F
authors.initials: F
authors.lastname: Chiarini
mesh_terms: D001483:Base Sequence; D001707:Biopsy, Needle; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002690:Chlamydia Infections / Q000175:diagnosis / Q000473:pathology; D003127:Colposcopy; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D011230:Precancerous Conditions / Q000382:microbiology* / Q000473:pathology*; D012680:Sensitivity and Specificity; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology*; D014626:Vaginal Smears / Q000592:standards