Electrical contacting of glucose oxidase by surface-reconstitution of the apo-protein on a relay-boronic acid-FAD cofactor monolayer.
A new methodology for the surface-reconstitution of apo-flavoenzymes on a relay unit (pyrroloquinoline quinone, PQQ) functionalized with a boronic acid ligand as a linker to native FAD was developed. The reconstitution of apo-glucose oxidase (apo-GOx) on the PQQ-FAD monolayer yields an electrically contacted enzyme-electrode.
Zayats
Maya
M
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Katz
Eugenii
E
Willner
Itamar
I
D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D010084:Oxidation-Reduction
D001897:Boronic Acids; D005182:Flavin-Adenine Dinucleotide; D005949:Glucose Oxidase