Characterization of the ves gene, which is expressed at a low temperature in Escherichia coli.
A gene, designated ves, that is expressionally responsive to temperature was found in Escherichia coli. Experiments with a single-copy lacZ operon fusion and primer extension analysis revealed that ves was expressed at a low temperature with a peak around 25 degrees C but was hardly expressed at 42 degrees C. After a temperature downshift, the mRNA level increased until 6 to 12 h and then decreased. Consistently, an A + T-rich sequence similar to UP elements seen in cold-shock inducible cold-shock protein (Csp) genes was found up-stream of the ves promoter, and its 5'-untranslated region was found to share similarity with those of the cold-shock inducible and cold-adaptive cspA and cspB genes. Additionally, a putative down-stream box, which also exists in cold-inducible proteins, was found. The ves product was identified by overproduction and determination of its N-terminal sequence. Similarity of the C-terminal portion of Ves to the CspA family suggests that Ves belongs to this family. The results of gene-disruption experiments suggest that ves is not essential for E. coli.
Yamada
Mamoru
M
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. yamada@agr.yamaguchi-u.ac.jp
Nagamitsu
Hiroshi
H
Izu
Hanae
H
Nakamura
Kazunori
K
Talukder
Ali Azam
AA
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003080:Cold Temperature; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013696:Temperature; D014158:Transcription, Genetic; D014764:Viral Proteins
D001426:Bacterial Proteins; D002352:Carrier Proteins; C491179:CspB protein, E coli; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014764:Viral Proteins; C062260:cold shock protein CS7.4, Bacteria; C077188:cold-shock protein CspB, Bacteria; C464972:ves protein, E coli; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases