A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus.
The deadly box jellyfish (Sea Wasp, Habu-kurage in Japanese) Chiropsalmus quadrigatus Haeckel (Cubozoa) is distributed widely in the tropical Pacific region. In Japan, three fatal cases due to stings from this species have been reported officially. We successfully isolated C. quadrigatus toxin-A (CqTX-A, 44 kDa), a major proteinaceous toxin, for the first time, from the nematocysts of C. quadrigatus. CqTX-A showed lethal toxicity to crayfish when administered via intraperitoneal injection (LD50 = 80 microg/kg) and hemolytic activity toward 0.8% sheep red blood cells (ED50 = 160 ng/ml). Furthermore, we sequenced the cDNA encoding CqTX-A. The deduced amino acid sequence of CqTX-A (462 amino acids) showed 25.2% and 21.6% sequence similarity to Carybdea rastoni toxins (CrTXs) and Carybdea alata toxin-A (CrTX-A), respectively, which are Cubozoan jellyfish toxins.
Nagai
Hiroshi
H
Suntory Institute for Bioorganic Research, Mishima, Osaka, Japan. nagai@tokyo-u-fish.ac.jp
Takuwa-Kuroda
Kyoko
K
Nakao
Masahiro
M
Oshiro
Naomasa
N
Iwanaga
Setsuko
S
Nakajima
Terumi
T
D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea; D001483:Base Sequence; D003064:Cnidarian Venoms / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D018076:DNA, Complementary; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D017433:Protein Structure, Secondary; D011506:Proteins / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity; D037401:Scyphozoa; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012756:Sheep
C465975:Chiropsalmus quadrigatus toxin-A; D003064:Cnidarian Venoms; D018076:DNA, Complementary; D011506:Proteins