title: Aspirin-sensitive versus non-aspirin-sensitive nasal polyp patients: analysis of leukotrienes/Fas and Fas-ligand expression.
abstract: OBJECTIVE
  The goal of the present study was to identify levels of leukotrienes released by leukocytes and nasal polyps from aspirin-sensitive patients (ASPs) and non-aspirin-sensitive patients (NASPs) after exposure to various concentrations of aspirin. Eosinophil expressions of apoptosis regulators were also compared.
  
  
  METHODS
  Leukotriene release from nasal polyps and leukocytes of ASPs and NASPs was measured after exposure to differing concentrations of aspirin. Eosinophils were analyzed for Fas and Fas ligand receptors using flow cytometry.
  
  
  RESULTS
  NASPs showed increased leukotriene release from nasal polyps on increased aspirin exposure. Polyps from ASPs did not show any change. ASPs showed greater leukotriene release from blood on increasing aspirin exposure compared with NASPs. Flow cytometric analysis showed eosinophils in the peripheral blood of ASPs have decreased Fas expression compared with those of NASPs.
  
  
  CONCLUSIONS
  This study showed differences in leukotriene release and eosinophil apoptosis between ASPs and NASPs.
  
  
  CONCLUSIONS
  These findings suggest a role of leukotrienes and eosinophils in aspirin sensitivity mechanisms in blood.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Stritch School of Medicine, Loyola University Medical Center, Maywood, Illinois 60153, USA.
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Ziroli
authors.forename: Hanjo
authors.initials: H
authors.lastname: Na
authors.forename: James M
authors.initials: JM
authors.lastname: Chow
authors.forename: James A
authors.initials: JA
authors.lastname: Stankiewicz
authors.forename: Max
authors.initials: M
authors.lastname: Samter
authors.forename: M Rita I
authors.initials: MR
authors.lastname: Young
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D017209:Apoptosis; D001241:Aspirin / Q000009:adverse effects*; D001249:Asthma / Q000276:immunology / Q000503:physiopathology; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015289:Leukotrienes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015289:Leukotrienes; D019014:fas Receptor; D001241:Aspirin