[Effects of IL-1 and TNF-alpha on the proliferation and cytosolic Ca2+ of lens epithelial cell in vitro].
OBJECTIVE
To study the effects of interleukin 1 (IL-1) and tumor necrosis factor alpha (TNF-alpha) on the proliferation and cytosolic Ca(2+) of bovine lens epithelial cells (BLEC) in vitro. The effects of IL-1 and TNF-alpha on posterior capsular opacification (PCO) and the mechanism of effect on the proliferation were investigated.


METHODS
The effects of IL-1 and TNF-alpha on the proliferation of BLEC were detected with MTT staining colorimetry, and cytosolic Ca(2+) was measured by fluorescence determination with Fura-2.


RESULTS
IL-1 10(2) - 10(5) ng/ml, TNF-alpha 10(2) - 10(4) U/ml obviously could promote BLEC proliferation (P < 0.05 - 0.01), and increase cytosolic Ca(2+) (P < 0.05 - 0.01) at the same doses.


CONCLUSIONS
IL-1, TNF-alpha contribute to the PCO. One of the mechanisms of IL-1 and TNF-alpha on proliferation is by cytosolic Ca(2+).
Zhang
F
F
Department of Ophthalmology, Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092.
Lu
D
D
Wang
L
L
Chen
C
C
Wu
N
N
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects; D007375:Interleukin-1 / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D002118:Calcium