Toward synthetic transcription activators: recruitment of transcription factors to DNA by a PNA-peptide chimera.
A PNA-peptide chimera designed to mimic the biochemical function of transcription activators has been synthesized and characterized. The bis-PNA segment binds specifically to a DNA site while the 20-residue peptide is capable of binding to the transcription factors Gal11 and Gal80. The PNA-peptide chimera thus mimics one of the central functions of a native transcription activator, recruitment of transcription factors to a specific DNA site.
Liu
Bo
B
Department of Internal Medicine and the Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, Texas 75390-8573, USA.
Han
Ying
Y
Corey
David R
DR
Kodadek
Thomas
T
D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018716:Molecular Mimicry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis* / Q000378:metabolism*; D015534:Trans-Activators / Q000138:chemical synthesis* / Q000378:metabolism*
D020135:Peptide Nucleic Acids; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D004247:DNA