Ribosomal dynamics explored by cryo-electron microscopy.
Cryo-electron microscopy has furnished direct evidence for conformational changes of the ribosome as it proceeds, in a cyclic manner, through different functional states. Strategies to explore the ribosome dynamics include trapping of particular functionally meaningful states by chemical, genetic, or physical means. The new atomic information obtained by X-ray crystallography should make it possible to track conformational changes observed by cryo-electron microscopy to changes of the underlying structural framework of the ribosome.
Frank
J
J
Howard Hughes Medical Institute, Albany, New York 12201-0509, USA. joachim@wadsworth.org
D020285:Cryoelectron Microscopy / Q000379:methods*; D018360:Crystallography, X-Ray / Q000379:methods; D008024:Ligands; D008958:Models, Molecular; D015233:Models, Statistical; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*
D008024:Ligands