Direct detection of isotopically labeled metabolites bound to a protein microarray using a charge-coupled device.
A charge-coupled device (CCD) was used to quantitatively detect isotope-labeled ligands bound to a protein microarray. Protein microarrays with protein dots, 10-50 microm in diameter, were fabricated on an aluminized Mylar film using an electrospray deposition technique. Proteins in dots were immobilized by cross-linking in glutaraldehyde vapor. After contact with solutions of isotope-labeled metabolites, the protein microarrays were washed, dried and placed face down onto the surface of a standard B/W video CCD chip with the protective window removed. We show here that such a simple inexpensive CCD detector can be used to quantify distribution of 14C and other radioactive isotopes on microarrays.
Morozov
Victor N
VN
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia. morozov@pbc.iteb.serpukhov.su
Gavryushkin
Alexander V
AV
Deev
Alexander A
AA
D001331:Automation; D001709:Biotechnology / Q000379:methods; D019992:Databases as Topic; D004563:Electrochemistry; D004583:Electrons; D007554:Isotopes / Q000494:pharmacology*; D008024:Ligands; D018715:Microscopy, Video; D036103:Nanotechnology; D020411:Oligonucleotide Array Sequence Analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*
D007554:Isotopes; D008024:Ligands; D011506:Proteins