title: Purification and quantification of lactoferrin in equine seminal plasma.
abstract: Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Purified lactoferrin was found to be highly homogeneous on the bases of its migration as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and of the monospecificity of rabbit antibodies to the purified protein in immunoblotting of seminal plasma proteins. A sandwich enzyme-linked immunosorbent assay was developed for quantifying lactoferrin in equine seminal plasma. Seminal plasma lactoferrin concentrations in 23 normal stallions ranged from 42 to 453 microg/ml, with a mean value of 157 +/- 118 microg/ml (S.D.).
authors.affiliation: Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Inagaki
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Orino
authors.forename: Yohji
authors.initials: Y
authors.lastname: Ohnami
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006736:Horses / Q000378:metabolism*; D007781:Lactoferrin / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D008970:Molecular Weight; D012661:Semen / Q000378:metabolism*
chemical_list: D007781:Lactoferrin