Morphometric and biomechanical remodelling in the intestine after small bowel resection in the rat.
The short-bowel syndrome is a clinical condition caused by intestinal resection. As intestinal adaptation occurs after resection, it can be used as a model for studying morphometric and biomechanical remodelling in the small intestine and to get a better understanding of the pathophysiology of the short-bowel syndrome. The resected rats had a 67% resection of jejunum and ileum. Control animals underwent no operation (nonoperated controls) or an ileal transection with subsequent end-to-end anastomosis (sham-resected controls). The animals were followed for up to 4 weeks after the operation. Changes in biomechanical properties were studied in terms of residual strain (the internal strain remaining when all external loads are removed), opening angle and stress--strain relations referenced to the zero-stress state (the cut-open state where external and internal stresses are released). The resected animals gained less weight than the controls. The intestinal length and diameter increased more in the resected groups than the control groups (P < 0.05), resulting in a larger absorptive surface. Resection induced profound gross morphometric changes and histological alterations characterized by proliferative increases in the tissue layers. The opening angle, along with residual strain at the mucosal and serosal surface, increased in the remnant small intestine (P < 0.05). All changes increased as function of postoperative time and were most prominent in the remnant ileum. However, the stress-strain relationship remained unchanged. In conclusion, this study demonstrated that resection of the majority of the small bowel results in significant remodelling in structural and residual strain properties in the remnant small intestine. The remodelling seems to be guided by the need for a greater absorptive surface area rather than for a change in the stress-strain properties.
Dou
Y
Y
Institute of Experimental Clinical Research, Aarhus University, Denmark.
Lu
X
X
Zhao
J
J
Gregersen
H
H
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001696:Biomechanical Phenomena; D006652:Histological Techniques; D007413:Intestinal Mucosa / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D007421:Intestine, Small / Q000473:pathology* / Q000503:physiopathology* / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000473:pathology / Q000503:physiopathology; D013312:Stress, Physiological / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D013997:Time Factors