title: Leishmania-induced cellular recruitment during the early inflammatory response: modulation of proinflammatory mediators.
abstract: This study investigated whether Leishmania species, the etiologic agent of cutaneous (Leishmania major) and visceral (Leishmania donovani) leishmaniasis, could differentially elicit early inflammatory events in vivo correlating with the subsequent development of their reciprocal pathogenesis. By use of the murine air pouch system, injection of Leishmania led to a rapid and transient accumulation of a mixed population of leukocytes, and L. major recruited 31-fold more leukocytes than did controls, compared with 7-fold more leukocytes for L. donovani. L. major promastigotes were better than L. donovani promastigotes at inducing proinflammatory cytokine secretion and chemokine gene expression in pouch exudates. L. major infection elicited significantly increased chemokine receptor gene expression, compared with L. donovani infection. Collectively, the data reveal that L. major is a strong inducer of the early inflammatory response, compared with L. donovani, and suggest that such an immunologic event potentially could restrain this parasite to the inoculation site, favoring the development of local swelling and cutaneous lesions.
authors.affiliation: Centre de Recherche en Infectiologie, CHU de Québec, Pavillon Centre Hospitalier de l'Université Laval, Faculté de Médecine, Université Laval, 2705 Boulevard Laurier, Sainte-Foy, Québec, Canada G1V 4G2. claudine.matte@yahoo.com
authors.forename: Claudine
authors.initials: C
authors.lastname: Matte
authors.forename: Martin
authors.initials: M
authors.lastname: Olivier
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D018836:Inflammation Mediators / Q000378:metabolism; D007893:Leishmania donovani / Q000276:immunology* / Q000472:pathogenicity; D018320:Leishmania major / Q000276:immunology* / Q000472:pathogenicity; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology; D007962:Leukocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*
chemical_list: D018836:Inflammation Mediators; D019707:Receptors, Chemokine