[Analysis of telomerase activity and telomere length in acute myelogenous leukemia].
OBJECTIVE
To study the changes and significance of telomerase activity and telomere length in acute myelogenous leukemia.


METHODS
TRAP-ELISA-PAGE was used to detect telomerase activity, Southern blot to estimate the length of telomere.


RESULTS
Telomerase activity was significantly higher in AML(Absorption(A): 2.298 +/- 1.059) than in normal control(A: 0.387 +/- 0.598) and the mean telomere length of AML [(7.6 +/- 2.1) kb] was significantly shorter than that of normal control[(9.3 +/- 1.9) kb]. The alteration of the telomere length was detected mainly in telomerase-positive AML.


CONCLUSIONS
It suggested that there was a close relationship between telomerase activity and telomere length in AML. The activation of telomerase might play an important role in the genesis and development of AML and telomere length changes may correlate with the activation of telomerase.
Liu
L
L
Department of Hematology, Tangdu Hospital, Fourth Millitary Medical University, Xi'an 710038, China.
Sun
B
B
Liang
Y
Y
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005347:Fibroblasts; D005819:Genetic Markers; D006367:HeLa Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology* / Q000235:genetics*; D012867:Skin / Q000166:cytology; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics*; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D019098:Telomerase