Fractionation of cytochromes of phototrophically grown Chloroflexus aurantiacus. Is there a cytochrome bc complex among them?
The cytochrome-containing membrane complexes of the phototrophically grown green non-sulfur bacterium Chloroflexus aurantiacus were fractionated by anion exchange chromatography. Three cytochrome b and four cytochrome c peaks were observed. None of the separated complexes met the features of the cytochrome bc complex. Two main cytochrome b-containing complexes were further purified: a dimer of identical subunits with unknown function and a succinate:quinone oxidoreductase containing three subunit species. Two novel multisubunit complexes, similar to each other, with two heme c-bearing subunits were also purified.
Yanyushin
Mikhail F
MF
Institute of Basic Biological Problems, Pushchino, 142 290, Moscow region, Russia. yanyushin@ibbp.psn.ru
D019414:Chlorobi / Q000737:chemistry* / Q000254:growth & development / Q000528:radiation effects; D003573:Cytochrome b Group / Q000302:isolation & purification*; D003574:Cytochrome c Group / Q000302:isolation & purification*; D003580:Cytochromes / Q000737:chemistry*
D003573:Cytochrome b Group; D003574:Cytochrome c Group; D003580:Cytochromes