Effect of endothelin-1 on hepatic damage induced by endotoxin.
OBJECTIVE: To observe effect of endothelin-1 (ET-1) on hepatic damage induced by endotoxin. METHODS: A total of 90 rats were randomly divided into control group (group C), endotoxin treated group (group LPS) and endotoxin plus ET-1 antibody treated group (group LEA). An observation was done on the changes of ET-1 concentration, and transcription and expression of ET-1 mRNA. Plasma glutamic pyruvic-transaminase enzyme (GPT), hepatic lactate dehydrogenase (LDH), adenosine triphosphate (ATP) and malondialdehyde (MDA) were also observed at 3, 6, 9, 12, 24 hours after saline, endotoxin (10 mg/kg) and ET-1 antibody (dalubine 1:2000, 2 ml/kg) administration. RESULTS: The results indicated that the concentration of plasma and hepatic ET-1 and expression of ET-1 mRNA in liver significantly increased following endotoxemia. The hepatic ET-1 levels were inversely correlated with the ATP concentration, and positively related to the MDA concentration. ET-1 antibody could partially protect the liver against damage induced by endotoxin. CONCLUSIONS: These results suggest that endotoxin may, on transcription and translation level, lead to an increase of ET-1 in synthesis. ET-1 may contribute to hepatic damage during endotoxemia.
Liu
Baohua
B
Department of General Surgery, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Xiao
Nan
N
Chen
Huisun
H
Zhou
Jihong
J