title: Development and validation of a reversed-phase high-performance liquid chromatographic method for the determination of ethyl-3-(N-n-butyl-N-acetyl)aminopropionate in an insect repellent semi-solid formulation.
abstract: A reversed-phase high-performance liquid chromatographic method with detection at 220 nm was developed and validated for the determination of ethyl-3-(N-n-butyl-N-acetyl)aminopropionate, IR 3535, in an insect repellent semi-solid product. A Hypersil ODS RP-C18 column (250 x 4.6 mm), 5 microm particle size, was equilibrated with a mobile phase consisted of water-acetonitrile (60:40, v/v). Its flow-rate was 1.0 ml/min. Excipients did not interfere with the determination of IR 3535 (Rs = 8.663). Intra- and inter-day relative standard deviations for samples were not higher than 0.61 and 1.2%, respectively. Mean recovery was found not lower than 98.5% and not higher than 100.3%. The method of external standard was adopted. Calibration curves were linear in the concentration range between 1.0 x 10(-6) and 5.0 x 10(-4) M. Limits of detection and quantitation were 65 and 196 ng/ml, respectively.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Greece.
authors.forename: S C
authors.initials: SC
authors.lastname: Marselos
authors.forename: H A
authors.initials: HA
authors.lastname: Archontaki
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007302:Insect Repellents / Q000737:chemistry*; D011422:Propionates / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007302:Insect Repellents; D011422:Propionates; C462400:ethyl-3-(N-n-butyl-N-acetyl)aminopropionate