Oligomerization of the Bacillus subtilis division protein DivIVA.
DivIVA appears to be a mediator of inhibition by MinCD of division at the cell poles in Bacillus subtilis. Gel permeation and ultracentrifugation techniques were used to show self-association of DivIVA into a form consisting of 10-12 monomers in vitro. Western blot analysis of non-denaturating polyacrylamide gels confirms the presence of similar oligomers in B. subtilis cell lysates. These oligomers persist in a B. subtilis strain containing the divIVA1 mutation, in which proper vegetative septum positioning is abolished. In contrast, the divIVA2 mutation, which has a similar biological impact, appears to produce a protein with different oligomerization properties. The results of the present study suggest that oligomerization of DivIVA is important, but not sufficient for its function in the cell division process.
Muchová
Katarı Na
KN
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
Kutejová
Eva
E
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
Scott
David J
DJ
Department of Biology, University of York, Heslington YO10 5YW, UK3.|Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
Brannigan
James A
JA
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
Lewis
Richard J
RJ
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
Wilkinson
Anthony J
AJ
Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
Barák
Imrich
I
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012394:Rosaniline Dyes; D014461:Ultracentrifugation
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C104636:DivIVA protein, bacteria; D012394:Rosaniline Dyes; C048139:Coomassie blue