title: EGb761 blocks MPP+-induced lipid peroxidation in mouse corpus striatum.
abstract: EGb761 has been suggested to be an antioxidant and free radical scavenger. Excess generation of free radicals, leading to lipid peroxidation (LP), has been proposed to play a role in the damage to striatal neurons induced by 1-methyl-4-phenylpyridinium (MPP+). We investigated the effects of EGb761 pretreatment on MPP+ neurotoxicity. C-57 black mice were pretreated with EGb761 for 17 days at different doses (0.63, 1.25, 2.5, 5 or 10 mg/kg) followed by administration of MPP+, (0.18, 0.36 or 0.72 mg/kg). LP was analyzed in corpus striatum at 30 min, 1 h, 2 h and 24 h after MPP+ administration. Striatal dopamine content was analyzed by HPLC at the highest EGb761 dose at 2 h and 24 h after MPP+ administration. MPP+-induced LP was blocked (100%) by EGb761 (10 mg/kg). Pretreatment with EGb761 partially prevented (32%) the dopamine-depleting effect of MPP+ at 24 h. These results suggest that supplements of EGb761 may be effective at preventing MPP+-induced oxidative stress.
authors.affiliation: Laboratory of Neurotoxicology, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez SS, México, DF, México.
authors.forename: P
authors.initials: P
authors.lastname: Rojas
authors.forename: B
authors.initials: B
authors.lastname: Garduño
authors.forename: C
authors.initials: C
authors.lastname: Rojas
authors.forename: R M
authors.initials: RM
authors.lastname: Vigueras
authors.forename: J
authors.initials: J
authors.lastname: Rojas-Castañeda
authors.forename: C
authors.initials: C
authors.lastname: Rios
authors.forename: N
authors.initials: N
authors.lastname: Serrano-Garcia
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology*; D020441:Ginkgo biloba; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009498:Neurotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D000096983:Ginkgo Extract
chemical_list: D015655:1-Methyl-4-phenylpyridinium; D009498:Neurotoxins; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract