Behavioural effects in rats after prenatal exposure to dearomatized white spirit.
The purpose of the study was to investigate the potential developmental neurotoxicity of the widely used organic solvent, white spirit. Rats (Mol:WIST) were exposed to 0 or 800 ppm dearomatized white spirit for 6 hr per day on gestation days 7-20. Developmental and neurobehavioural effects in the offspring were investigated using a test battery including assessment of physical development, reflex ontogeny, motor function, motor activity and, learning and memory. No significant effects were recorded on motor function and the activity in Open Field. In the initial learning period (age 1 month), the performance in a Morris water maze was similar in exposed and control animals. When testing for memory at the age of 2 months, the exposed male offspring used more time to locate the hidden platform. After platform relocation, impaired cognitive function was revealed in the exposed females. At the age of 5 months, learning and memory deficits were observed in exposed offspring. The differences were not related to poorer swimming capabilities, because swim speeds were similar to control values. The results show that prenatal exposure to 800 ppm white spirit caused long-lasting learning and memory deficits in rats.
Hass
U
U
Department of Chemical Working Environments, National Institute of Occupational Health, Copenhagen, Denmark. ulh@fdir.dk
Ladefoged
O
O
Lam
H R
HR
Ostergaard
G
G
Lund
S P
SP
Sinonsen
L
L
D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005106:Exploratory Behavior / Q000187:drug effects; D005260:Female; D006838:Hydrocarbons / Q000633:toxicity*; D018782:Maze Learning / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D012098:Reproduction / Q000187:drug effects; D012997:Solvents / Q000633:toxicity*; D013550:Swimming; D018675:Toxicity Tests
D006838:Hydrocarbons; D012997:Solvents