title: Induction of global stress response in Saccharomyces cerevisiae cells lacking telomerase.
abstract: Cellular senescence is a major intermediate step from healthy cells toward tumor cells. By using microarrays that simultaneously examine the transcription levels of 6,200 Saccharomyces cerevisiae genes, we show that 45 gene transcript levels are increased and 11 are decreased after exposure to telomere shortening and cellular senescence in a telomerase-deficient mutant. About half of the genes that showed increased expression were found induced under stress, consistent with the notion that critical short telomeres cause stress to cells. Surprisingly, the expression level of telomere recombination genes was not altered suggesting that even though recombination is a means to rescue critically short telomeres, its machinery was not controlled by telomere shortening. The expression of telomere-proximal genes was also analyzed. The possibility of induction of a program to cope with cellular senescence and active telomere-telomere recombination is discussed.
authors.affiliation: Department of Microbiology, National Taiwan University College of Medicine, Taipei, 10018, Taiwan. scteng@ha.mc.ntu.edu.tw
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Teng
authors.forename: Charles
authors.initials: C
authors.lastname: Epstein
authors.forename: Yun-Luen
authors.initials: YL
authors.lastname: Tsai
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Cheng
authors.forename: Hung-Lin
authors.initials: HL
authors.lastname: Chen
authors.forename: Jing-Jer
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D016922:Cellular Senescence; D015536:Down-Regulation; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012331:RNA, Fungal / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics; D019098:Telomerase / Q000235:genetics / Q000502:physiology*; D016615:Telomere; D015533:Transcriptional Activation
chemical_list: D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D019098:Telomerase