Antioxidant systems of the developing quail embryo are compromised by mycotoxin aurofusarin.
The effects of aurofusarin in the quail diet on the antioxidant systems of the developing embryo are investigated. Thirty eight 45-day-old Japanese quails (Coturnix japonica) were divided into two groups and were fed on a corn-soya diet or the same diet supplemented with aurofusarin at the level of 26.4 mg/kg feed in the form of Fusarium graminearum culture enriched with aurofusarin. Eggs obtained after 7 weeks of feeding were incubated. Samples of quail tissues were collected at day 17 of embryonic development and from day old hatchlings. Antioxidants and malondialdehyde were analysed by HPLC-based methods. Inclusion of aurofusarin in the maternal diet was associated with decreased concentrations of alpha- and gamma-tocopherols, alpha- and gamma-tocotrienols, retinol, lutein and zeaxanthin in egg yolk. The vitamin E (tocopherols and tocotrienols) concentration in the liver and yolk sac membrane (YSM) of the day 17 embryos and the hatchlings from aurofusarin-fed group was significantly decreased. Alpha-tocopherol concentration was also reduced in kidney, lung, heart, muscle and brain of day-old quails. In the liver of day-old quails, concentrations of lutein, zeaxanthin, retinol, retinyl linoleate, retinyl oleate, retinyl palmitate and retinyl stearate were also reduced. As a result of these diminished antioxidant concentrations, tissue susceptibility to lipid peroxidation was significantly increased. It is suggested that a compromised antioxidant system of the egg yolk and embryonic tissues could predispose quails to increased mortality at late stages of their embryonic development.
Dvorska
Julia E
JE
Department of Veterinary Medicine, Sumy State Agrarian University, Ukraine.
Surai
Peter F
PF
Speake
Brian K
BK
Sparks
Nick H C
NH
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D003370:Coturnix; D004530:Egg Yolk / Q000737:chemistry / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000737:chemistry / Q000196:embryology / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D009183:Mycotoxins / Q000633:toxicity*; D009285:Naphthoquinones / Q000633:toxicity*; D011784:Quail / Q000196:embryology*; D014018:Tissue Distribution; D014810:Vitamin E / Q000032:analysis / Q000378:metabolism; D015017:Yolk Sac / Q000737:chemistry / Q000378:metabolism
D000975:Antioxidants; D009183:Mycotoxins; D009285:Naphthoquinones; D014810:Vitamin E; D002338:Carotenoids; D008315:Malondialdehyde; C083578:aurofusarin