[Inhibition of monocytes adhesion to the intima of arterial wall by local expression of antisense monocyte chemotactic protein-1].
OBJECTIVE
To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process.


METHODS
Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.


RESULTS
Expression of antisense MCP-1 mediated by recombinant LNCX plasmid/lipsomal complex gene transfer enabled to inhibit MCP-1 gene expression and adhesion of monocyte to the intima.


CONCLUSIONS
MCP-1 plays an important role on the recruitment of monocytes in the arterial wall, which provides a potential clue in developing a gene therapy project for the prevention and treatment of atherogenesis.
Wu
Q
Q
Department of Pathology, Institute of Basic Medical Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100005, China.
Qiao
H
H
Wang
Z
Z
Zhang
H
H
Liu
P
P
Xu
M
M
Ren
G
G
Zhao
S
S
She
M
M
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology*; D002448:Cell Adhesion; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D016373:DNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D005263:Femoral Artery / Q000648:ultrastructure*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D017539:Tunica Intima / Q000648:ultrastructure
D018932:Chemokine CCL2; D016373:DNA, Antisense; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein