title: Measurement of blood volume after haemodilution with haemoglobin-based oxygen carriers by a radiolabelled-albumin method.
abstract: Recent studies have shown that the use of haemoglobin-based oxygen-carrying solutions (HBOCs) for perioperative haemodilution could significantly reduce the need for packed red blood cells in clinical practice. Though the effects of HBOCs on plasma volume have been characterized in experimental models of volume resuscitation from hypovolaemic shock, little is known about their action in normovolaemic haemodilution conditions. We therefore applied a radiolabelled serumalbumin method to determine blood volume after haemodilution with crosslinked or conjugated haemoglobin, in comparison with a reference solution of hydroxyethyl starch (HES). Three groups of New Zealand white rabbits were studied (n = 7 each group) subjected to moderate exchange transfusion with low molecular weight HES, bis(3,5-dibromosalicyl)fumarate crosslinked haemoglobin (alphaalpha-Hb), or dextran-conjugated haemoglobin (Hb-Dex-BTC). HES induced no changes in heart rate and blood pressure. The amplitude and duration of blood pressure increase and bradycardia were similar in both haemoglobin groups. A significant contraction of blood volume (12%) was observed 60 min after haemodilution with alphaalpha-Hb, compared to HES and Hb-Dex-BTC. At the same time point, a decrease in absolute haemoglobin (plasma haemoglobin x plasma volume) was also noted. This study suggests that in haemodilution conditions, the specific oncotic properties and circulating persistence of crosslinked and conjugated haemoglobin solutions affect the pattern of blood volume distribution differently.
authors.affiliation: Department of Haematology and Physiology, University Henri Poincaré-Nancy 1, 5 rue Albert Lebrun, 54001 Nancy cedex, France. caron@pharma.u-nancy.fr
authors.forename: A
authors.initials: A
authors.lastname: Caron
authors.forename: J C
authors.initials: JC
authors.lastname: Mayer
authors.forename: P
authors.initials: P
authors.lastname: Menu
authors.forename: A
authors.initials: A
authors.lastname: Alayash
authors.forename: P Y
authors.initials: PY
authors.lastname: Marie
authors.forename: C
authors.initials: C
authors.lastname: Vigneron
mesh_terms: D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects; D001802:Blood Substitutes / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D001810:Blood Volume; D003911:Dextrans / Q000494:pharmacology; D005078:Exchange Transfusion, Whole Blood / Q000379:methods; D006339:Heart Rate / Q000187:drug effects; D006438:Hemodilution / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis / Q000494:pharmacology; D011817:Rabbits; D011849:Radioactive Tracers; D012709:Serum Albumin
chemical_list: D001802:Blood Substitutes; D003911:Dextrans; D006454:Hemoglobins; D011849:Radioactive Tracers; D012709:Serum Albumin; C053936:hemoglobin XL99alpha; C095659:hemoglobin-dextran 10-benzene-tetracarboxylate; D001241:Aspirin