Effects of calcium phosphate ceramic bone graft materials on permanent teeth eruption in beagles.
OBJECTIVE
The aim of this study was to evaluate the use of calcium phosphate ceramic (CPC) materials as a potential alternative to autogenous secondary alveolar bone grafting in cleft lip and palate patients who are in mixed dentition.


METHODS
Four 12-week-old beagles and one 15-week-old beagle were used as subjects.


METHODS
In each experimental beagle, the third and fourth deciduous premolars were extracted. The sockets were filled with four different CPC materials and sutured. MAIN OUTCOME MEASURES The beagles were fed a soft diet for the following 8 weeks and then sacrificed for clinical, radiological, histological, transmission electron microscope (TEM), and infrared (IR) absorption analysis.


RESULTS
All four experimental graft materials allowed normal development and eruption of permanent premolars. In histological sections, small particles of biphasic calcium phosphate (BCP) and carbonate apatite (CO(3)-AP) were resorbed, and large particulate forms served as bone frames in cortical bones. Polymer coated with carbonate apatite (Poly/CO(3)-AP) did not cause inflammation but was pushed away to the soft tissue by erupting teeth. Alginate coated with carbonate apatite (alginate/CO(3)-AP) caused a severe inflammatory reaction to the point of destroying a part of the dental follicle and cortical bone. In TEM, resorption activity by phagocytic cells was observed only in CO(3)-AP. Direct bonding of CO(3)-AP to the bone was observed as the electron-dense interface between bone and CO(3)-AP.


CONCLUSIONS
BCP and CO(3)-AP proved to be suitable as alveolar bone graft materials in areas where tooth eruption occurs. Of the four materials tested, CO(3)-AP produced the best results.
Linton
Jina Lee
JL
Department of Orthodontics, College of Dentistry, Yonsei University, Seoul, South Korea. jinalinton@netsgo.com.
Sohn
Byung-Wha
BW
Yook
Jong-In
JI
Le Geros
Racquel Z
RZ
D000464:Alginates / Q000009:adverse effects / Q000737:chemistry; D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000187:drug effects / Q000648:ultrastructure; D000543:Alveoloplasty; D000818:Animals; D001031:Apatites / Q000737:chemistry / Q000627:therapeutic use; D001641:Bicuspid / Q000502:physiology / Q000601:surgery; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000737:chemistry / Q000627:therapeutic use*; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use*; D003795:Dental Sac / Q000187:drug effects; D004285:Dogs; D005500:Follow-Up Studies; D019344:Lactic Acid / Q000737:chemistry / Q000627:therapeutic use; D008854:Microscopy, Electron; D009805:Odontogenesis / Q000502:physiology; D010586:Phagocytes / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use; D011859:Radiography; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D014078:Tooth Eruption / Q000502:physiology*; D014081:Tooth Extraction; D020390:Tooth Socket / Q000601:surgery; D014094:Tooth, Deciduous / Q000601:surgery
D000464:Alginates; D001031:Apatites; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D011108:Polymers; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C030782:carboapatite; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous