title: Screening approach for chiral separation of pharmaceuticals. Part I. Normal-phase liquid chromatography.
abstract: A strategy for rapid screening for the separation of chiral molecules of pharmaceutical interest by normal-phase liquid chromatography using three cellulose/amylose stationary phases is proposed. In a first step, the most important parameters for the separations were determined and studied for their effects by means of experimental designs. Results showed that the cellulose tris-(3,5-dimethylphenylcarbamate), the amylose tris-(3,5-dimethylphenylcarbamate) and the cellulose tris-(4-methylbenzoate) stationary phases have very broad and complementary enantiorecognition properties. The type of organic modifier used in the mobile phase appeared to have a dramatic influence on the quality of the separation. Based on the results of the preliminary study, a screening strategy was developed and successfully applied to a set of 36 diverse drugs. Enantiomeric separation was observed in 89% of cases and the analysis times were usually shorter than 20 min.
authors.affiliation: Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Perrin
authors.forename: V A
authors.initials: VA
authors.lastname: Vu
authors.forename: N
authors.initials: N
authors.lastname: Matthijs
authors.forename: M
authors.initials: M
authors.lastname: Maftouh
authors.forename: D L
authors.initials: DL
authors.lastname: Massart
authors.forename: Heyden Y
authors.initials: HY
authors.lastname: Vander
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations