Determination of triton X-100 in plasma-derived coagulation factor VIII and factor IX products by reversed-phase high-performance liquid chromatography.
Plasma protein pools are often virus-inactivated by the solvent-detergent method, using tri-n-butyl phosphate and Triton X-100, followed by removal and determination of these compounds. We used reversed-phase high-performance liquid chromatography for the determination of Triton X-100 in coagulation factor VIII and factor IX products, Octonativ-M and Nanotiv, respectively (Pharmacia, Stockholm, Sweden). The chromatographic system included a C18 silica column and a linear acetonitrile gradient. The advantage of this method is the low detection limit (0.3 microg/ml) combined with detection at 280 nm, which gives a more stable baseline and has less interference from other compounds. As compared to other methods, where shorter wavelengths are used.
Karlsson
Göran
G
Plasma R&D, Biovitrum, Stockholm, Sweden. goran.karlsson@biovitrum.com
Hinz
Ann-Charlotte
AC
Henriksson
Elisabeth
E
Winge
Stefan
S
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005164:Factor IX / Q000737:chemistry*; D005169:Factor VIII / Q000737:chemistry*; D017830:Octoxynol / Q000032:analysis*
D005169:Factor VIII; D005164:Factor IX; D017830:Octoxynol