Rapid determination of nevirapine in human plasma by gas chromatography.
A sensitive and rapid gas chromatographic method has been developed to determine the levels of the HIV-1 non-nucleoside reverse transcriptase inhibitor nevirapine in human plasma. Quantitative recovery following liquid-liquid-extraction with diethylether from 500 microl of human plasma was achieved. Subsequently, the assay was performed with a CP-Sil 5CB capillary column, 15 m x 0.32 mm x 1.0 microm film thickness with a nitrogen-phosphorous-detector (NPD), Helium 5.0 was used as carrier gas with a constant inlet pressure of 7 p.s.i. Linear standard curves were obtained for concentrations ranging from 10 to 20 000 ng/ml. The calculated intra- and inter-day coefficients of variation were below 8%.
Langmann
Peter
P
Department of Internal Medicine, University of Wuerzburg, Germany. p.langmann@medizin.uni-wuerzburg.de
Schirmer
Diana
D
Väth
Thomas
T
Desch
Steffen
S
Zilly
Michael
M
Klinker
Hartwig
H
D002849:Chromatography, Gas / Q000379:methods*; D015658:HIV Infections / Q000097:blood; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity
D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine