Automated measurements of antilisterial activities of lactate and diacetate in ready-to-eat meat.
Standard procedures to enumerate Listeria organisms rely on plating food samples on selective agar media. The procedures are labor-intensive, and because of the limited sensitivity, pre-enrichment step is required for the detection of low numbers of the pathogen. In the present study, an automated rapid optic procedure and the standard procedure were used to determine the behavior of the pathogen in ready-to-eat (RTE) meat and to test the effect of antilisterial agents. Listeria monocytogenes strain Scott A or a six-strain mixture of Listeria was studied using lactate (2.5%), diacetate (0.2%) and their combination in beef bologna and in sterile beef emulsion. Samples stored for up to 60 days at 5 and 10 degrees C were tested at time intervals during storage. Using the plate count method, each of the salts caused a delay in growth of the pathogen, and the salt combination was most effective causing listeriostatic effects and decline in growth of the pathogen at 5 degrees C. High negative correlation (r), ranging from 0.92 to 0.99, was obtained between the detection time (DT) recorded by the optic procedure (BioSys instrument) and cell numbers determined by the plate count procedure. The rapid (< 24 h) optic procedure was reliable in assessing the efficacy of antimicrobials and in rapid detection of low levels of listeriae that are undetectable by direct plating procedure.
Mbandi
Evelyne
E
Department of Nutrition and Food Science, College of Science, Wayne State University, Detroit, MI 48202, USA.
Shelef
Leora A
LA
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000472:pathogenicity; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology*; D055095:Optics and Photonics; D019354:Sodium Lactate / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
D000085:Acetates; D019354:Sodium Lactate