title: Keratocyte density of central human cornea after laser in situ keratomileusis.
abstract: OBJECTIVE
  To determine changes in keratocyte density in the first year after laser in situ keratomileusis (LASIK).
  
  
  METHODS
  Prospective interventional cohort study.
  
  
  METHODS
  Seventeen eyes of 11 patients received LASIK with a planned 180-microm flap to correct refractive errors between -2.0 diopters and -11.0 diopters. Images of the full-thickness cornea were obtained by using confocal microscopy in vivo before LASIK and at 1 week, 1, 3, 6, and 12 months after LASIK. Bright objects (that resembled keratocytes) in images without motion blur were manually counted by one observer. Cell densities were determined in anterior and posterior halves of the stromal flap, anterior and posterior halves of the layer 100 microm-thick immediately deep to the ablation (retroablation layer), and in the posterior third of the stroma. The region of stroma that was ablated (as measured 1 month after LASIK) was omitted from the preoperative analysis.
  
  
  RESULTS
  Keratocyte density in the anterior flap was 28,978 +/- 5849 cells/mm(3) (mean +/- SD) pre-LASIK, and was decreased at all postoperative examinations, but the difference was not significant until 12 months after LASIK (22% decrease). Keratocyte densities in the posterior flap were 20,397 +/- 4215 cells/mm(3) pre-LASIK and were decreased by 20%-40% at all postoperative examinations 1 week to 1 year after LASIK. Keratocyte densities in the anterior half of the retroablation layer were 16,605 +/- 3595 cells/mm(3) pre-LASIK and decreased by 16%-30% between 3 and 12 months after LASIK. Keratocyte densities in the posterior half of the retroablation layer and posterior stroma did not change.
  
  
  CONCLUSIONS
  Keratocyte densities in the posterior flap and anterior retroablation layer (regions adjacent to the lamellar cut) decrease at 1 week and 3 months, respectively, after LASIK and remain decreased in these regions at 12 months after LASIK. In the anterior flap, keratocyte density decreases 1 year after LASIK. The long-term effects of these cellular deficits, if any, require further study.
authors.affiliation: Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Mitooka
authors.forename: Manuel
authors.initials: M
authors.lastname: Ramirez
authors.forename: Leo J
authors.initials: LJ
authors.lastname: Maguire
authors.forename: Jay C
authors.initials: JC
authors.lastname: Erie
authors.forename: Sanjay V
authors.initials: SV
authors.lastname: Patel
authors.forename: Jay W
authors.initials: JW
authors.lastname: McLaren
authors.forename: David O
authors.initials: DO
authors.lastname: Hodge
authors.forename: William M
authors.initials: WM
authors.lastname: Bourne
mesh_terms: D000328:Adult; D001251:Astigmatism / Q000601:surgery; D002452:Cell Count; D003319:Corneal Stroma / Q000294:innervation / Q000473:pathology* / Q000601:surgery; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D020731:Keratomileusis, Laser In Situ; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009216:Myopia / Q000601:surgery; D009412:Nerve Fibers; D011446:Prospective Studies; D013524:Surgical Flaps; D013997:Time Factors