High-performance liquid chromatographic assay for the simultaneous determination of sulfadoxine and pyrimethamine from whole blood dried onto filter paper.
A method using solid-phase extraction and high-performance liquid chromatography is evaluated for the simultaneous determination of sulfadoxine and pyrimethamine from 0.1 ml of whole blood dried onto filter paper. Extraction recoveries are about 60% for both drugs. The coefficient of variation for intra-assay precision, inter-assay precision and accuracy is less than 10% for sulfadoxine (10-100 microg/ml) and pyrimethamine (1-10 microg/ml).
Green
Michael D
MD
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, Atlanta, GA 30333, USA. mdg4@cdc.gov
Mount
Dwight L
DL
Nettey
Henry
H
D000962:Antimalarials / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011739:Pyrimethamine / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013413:Sulfadoxine / Q000097:blood*
D000962:Antimalarials; D013413:Sulfadoxine; D011739:Pyrimethamine