Molecular characterization of a Spanish isolate of Mycobacterium malmoense.
We present the genomic characterization of a Spanish isolate of Mycobacterium malmoense. Identification and molecular characterization of the microorganism were performed using the technique of PCR-restriction fragment length polymorphism analysis (PRA) and sequencing of the 16S and the ITS spacer (16S-23S rDNA intergenic spacer region). It was seen that our isolate is very similar to the strain described in Sweden, but differs in the sequencing of the ITS from the isolate in Great Britain. Molecular techniques facilitate a rapid diagnosis and enable a better understanding of the epidemiology of this microorganism.
Ruiz
Montserrat
M
Servicio de Microbiología, Hospital General Universitario de Elche, Alicante, Spain.
Rodríguez
Juan Carlos
JC
García-Martínez
Jesús
J
Escribano
Isabel
I
Sirvent
Elia
E
Gutiérrez
Félix
F
Rodríguez-Valera
Francisco
F
Royo
Gloria
G
D017088:AIDS-Related Opportunistic Infections / Q000453:epidemiology* / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000453:epidemiology* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain / Q000453:epidemiology
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S