Fas ligand mRNA expression in the mouse central nervous system.
Fas ligand (FasL) expressing cells delete Fas bearing T cells, thereby enabling privileged immune status in the brain. Although the presence of FasL immunoreactivity has been shown in various cell types in the central nervous system, the precise in vivo distribution of FasL mRNA in mammals is not known. Accordingly, we localized intense FasL mRNA signals in neuroglial cells mainly within the white matter regions. Using a combined labeling technique of immunocytochemistry and in situ hybridization, we confirmed that FasL signals were due to neuroglial cells rather than neurons. This study shows that FasL mRNA is constitutively expressed in the normal mouse brain, and suggests that the Fas/FasL system protects the CNS from immunological damage.
Shin
Dong Hoon
DH
Department of Anatomy, Dankook University College of Medicine, Chonan, South Korea.
Lee
Eunju
E
Kim
Hyun Joon
HJ
Kim
Seonghan
S
Cho
Sa Sun
SS
Chang
Ka Young
KY
Lee
Wang Jae
WJ
D000818:Animals; D001921:Brain / Q000378:metabolism*; D053222:Fas Ligand Protein; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000032:analysis*
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger