title: An ankyrin repeat-containing protein plays a role in both disease resistance and antioxidation metabolism.
abstract: The Arabidopsis ankyrin repeat-containing protein AKR2 was identified as a GF14(lambda)-interacting protein in a yeast two-hybrid screening (GF14(lambda) is a 14-3-3 protein). Reduced expression of AKR2 by using the antisense technique results in small necrotic areas in leaves accompanied by higher production of H2O2, similar to the hypersensitive response to pathogen infection in plant disease resistance. Transcripts of genes encoding pathogen-induced protein PR-1 (pathogen-related protein 1) and stress-responsive protein GST6 (glutathione S-transferase 6) are increased in antisense plants. The resistance to a bacterial pathogen infection was also increased by at least 10-fold in antisense plants. AKR2 also interacts with another GF14(lambda)-interacting protein, the ascorbate peroxidase 3 that scavenges H2O2 in plant cells. These data suggest that AKR2 may be a negative regulator of PR-1 expression, and is probably involved in the regulation of antioxidation metabolism that is shared by both disease resistance and stress responses.
authors.affiliation: Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA.
authors.forename: Juqiang
authors.initials: J
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D017089:Ankyrin Repeat / Q000235:genetics* / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000502:physiology; D060387:Ascorbate Peroxidases; D001665:Binding Sites; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D020672:I-kappa B Proteins; D007113:Immunity, Innate; D008565:Membrane Proteins; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D019151:Peptide Library; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C451699:AKR2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D016328:NF-kappa B; D019151:Peptide Library; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D000072000:NF-KappaB Inhibitor alpha; C077262:PR-1 protein, Arabidopsis; D006861:Hydrogen Peroxide; D010544:Peroxidases; C555294:APX3 protein, Arabidopsis; D060387:Ascorbate Peroxidases