title: [Detection of hypermethylation of p15 gene in patients with acute leukemia by polymerase chain reaction].
abstract: OBJECTIVE
  To explore the role of hypermethylation of p15 gene in the pathogenesis of acute leukemias.
  
  
  METHODS
  DNA from 36 cases of acute leukemia were investigated using restriction enzyme digestion combined with polymerase chain reaction (PCR) technique.
  
  
  RESULTS
  Hypermethylations of p15 gene were found in 22 (61.1%) patients, including 17 cases of acute non-lymphoblastic leukemia (ANLL) and 5 of acute lymphoblastic leukemia (ALL). ANLL patients exhibited p15 gene hypermethylations had higher WBC in peripheral blood (P < 0.05) and higher percentages of leukemia cells in bone marrow (P < 0.01).
  
  
  CONCLUSIONS
  Inactivation of p15 gene due to hypermethylation may be involved in the pathogenesis of some acute leukemias.
authors.affiliation: Department of Hematology, the Second Affiliated Hospital, Hubei Medical University, Wuhan 430071, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D018797:Cell Cycle Proteins / Q000235:genetics*; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology; D025521:Tumor Suppressor Proteins
chemical_list: C493966:CDKN2B protein, human; D018797:Cell Cycle Proteins; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D025521:Tumor Suppressor Proteins