title: Evaluation of select variables in the ion chromatographic determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples.
abstract: A full experimental design at two levels is applied for the estimation of the significance of select factors that may influence the ion chromatography (IC) determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples. The factors studied are various sample deproteinization procedures, eluent composition, and flow rates. Deproteinization using either acetonitrile-NaOH or ultrafiltration can be used in order to obtain a significant protein removal before IC analysis; however, the former is recommended because it is less time-consuming and cheaper. Better resolution is obtained when a sodium hydroxide solution is used as the eluent. There is no influence of the sample's deproteinization procedures on the chromatographic resolution.
authors.affiliation: Centro de Química, Instituto Venezolano de Investigaciones Científicas, IVIC, Caracas, Venezuela. zbenzo@ivic.ve
authors.forename: Z
authors.initials: Z
authors.lastname: Benzo
authors.forename: A
authors.initials: A
authors.lastname: Escalona
authors.forename: J
authors.initials: J
authors.lastname: Salas
authors.forename: C
authors.initials: C
authors.lastname: Gómez
authors.forename: M
authors.initials: M
authors.lastname: Quintal
authors.forename: E
authors.initials: E
authors.lastname: Marcano
authors.forename: F
authors.initials: F
authors.lastname: Ruiz
authors.forename: A
authors.initials: A
authors.lastname: Garaboto
authors.forename: F
authors.initials: F
authors.lastname: Bartoli
mesh_terms: D000838:Anions / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans
chemical_list: D000838:Anions