Establishment and characterization of a cell line from a malignant fibrous histiocytoma of bone developing in a patient with multiple fibrous dysplasia.
OBJECTIVE
In order to provide material for genetic analysis of fibrous dysplasia (FD), a cell line designated GBS-1 was established from a secondary bone malignant fibrous histiocytoma (MFH) developing in a rib of a 44-year-old male polyostotic FD patient.


RESULTS
The GBS-1 cells are characterized by a pleomorphic spindle cell morphology with abundant mucus production. On transplantation to nude mouse subcutis the cell line forms myxoid-spindle cell sarcomas with giant cells, the myxoid product being positive for periodic acid-Schiff (PAS) and alcian blue (Al-B) stains and completely digested by hyaluronidase, mimicking the original tumor. Chromosome and genetic analyses revealed multiple structural and numerical abnormalities of chromosomes with a large number of unidentifiable chromosomes and p53 mutation in exon 7 with LOH in the counterpart.


CONCLUSIONS
Since cell lines for FD have hitherto not been available, the GBS-1 cells should prove useful for genetic analyses of FD and also MFH of bone origin.
Fang
Z
Z
Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan.
Mukai
H
H
Nomura
K
K
Shinomiya
K
K
Matsumoto
S
S
Kawaguchi
N
N
Kitagawa
T
T
Kanda
H
H
D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002874:Chromosome Mapping; D018450:Disease Progression; D005357:Fibrous Dysplasia of Bone / Q000473:pathology*; D016158:Genes, p53; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000473:pathology*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured