title: High-performance liquid chromatography with spectrophotometric and electrochemical detection of a series of manganese(III) cationic porphyrins.
abstract: Recent studies have revealed potent pharmacological activities of manganese-containing cationic porphyrins. An analytical method employing high-performance liquid chromatography with spectrophotometric and electrochemical detection (HPLC-UV/EC) suitable for in vivo applications is described for a series of manganese(III) cationic porphyrins with good separation and resolution. In particular, this method resolved the four atropisomers of manganese(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP5+ or AEOL-10113), verified by mass spectrometry. Electrochemical and spectrophotometric methods of detection were compared using manganese(III) meso-tetrakis(1,3-diethylimidazolium-2-yl)porphyrin (MnTDE-2-ImP5+ or AEOL-10150), the lead catalytic antioxidant of this series. Both methods of detection were quantitative, but electrochemical detection, although less specific for in vivo applications, appears to be considerably more sensitive than spectrophotometric detection.
authors.affiliation: Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA
authors.forename: Remy
authors.initials: R
authors.lastname: Kachadourian
authors.forename: Ramil
authors.initials: R
authors.lastname: Menzeleev
authors.forename: Bushra
authors.initials: B
authors.lastname: Agha
authors.forename: Steve B
authors.initials: SB
authors.lastname: Bocckino
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Day
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008345:Manganese / Q000737:chemistry*; D008665:Metalloporphyrins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D008665:Metalloporphyrins; D008345:Manganese