Single-copy nuclear DNA sequences obtained from noninvasively collected primate feces.
Noninvasively collected primate feces have been shown to provide a useful source of mitochondrial DNA for sequencing and nuclear microsatellite DNA for size analysis. In this study, single-copy nuclear DNA sequences were obtained from noninvasively collected fecal samples of two species of wild tamarins, Saguinus fuscicollis and S. mystax, in the context of a project on the functional utility of color vision. Noninvasive genotyping of the X-linked opsin gene is important for future studies of selection and adaptation at this locus in a number of primate species. The wide range of techniques that can now be applied successfully to DNA extracted from feces introduces a broad spectrum of potential genetic studies that can be undertaken on primates, without the need for intrusive or invasive methods.
Surridge
Alison K
AK
Institute of Biological Anthropology, University of Oxford, Oxford, United Kingdom.
Smith
Andrew C
AC
Buchanan-Smith
Hannah M
HM
Mundy
Nicholas I
NI
D000818:Animals; D000835:Animals, Wild; D002467:Cell Nucleus / Q000235:genetics; D003118:Color Perception; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005243:Feces; D005260:Female; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D012452:Saguinus / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000662:veterinary*; D013048:Specimen Handling
D017931:DNA Primers; D004272:DNA, Mitochondrial