title: MRI volume measurements of hypointense objects. A phantom study using stereological methods.
abstract: Volume estimates made from thick slabs overestimate the volume of a positive contrast particle in a translucent matrix and underestimate its volume if the particle has negative contrast and the matrix is opaque. For T2-weighted MRI high signal objects the bias can be corrected according to simple geometric models. For negative contrast or hypointense objects the magnitude of the bias is unknown and no corrections have yet been put forward. We wanted to determine the bias of MRI measurements of hypointense objects of known diameter surrounded by a positive contrast solution. One, 3 and 5 mm slices with three different contrast concentrations were obtained according to a stereological MRI protocol. Results were compared with the true object size to estimate the bias. A simple correction for the bias could not be developed due to interslice cross-talk. Cross-talk makes an object appear in more MRI slices than corresponding to its physical size and larger than the true diameter. For phantom measurements an empirical correction could be developed, but for measurements of non-ideal objects such as brain structures the validity of the correction would be unpredictable. Besides avoiding or reducing cross-talk, the best way to deal with this bias is to measure the object's maximal contrast from edge to edge, a solution which is not perfect.
authors.affiliation: Department of Neurology, Aarhus University Hospital, Aarhus University, Aarhus, Denmark. paula@akhphd.au.dk
authors.forename: Paula
authors.initials: P
authors.lastname: Gadeberg
authors.forename: Hans J G
authors.initials: HJ
authors.lastname: Gundersen
authors.forename: Finn
authors.initials: F
authors.lastname: Taagehøj
authors.forename: Johannes
authors.initials: J
authors.lastname: Jakobsen
mesh_terms: D008279:Magnetic Resonance Imaging / Q000295:instrumentation* / Q000379:methods; D019047:Phantoms, Imaging / Q000706:statistics & numerical data; D013238:Stereotaxic Techniques / Q000295:instrumentation / Q000706:statistics & numerical data