Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA.
Novel redox-active DNA labeling tags with tunable electrochemical potentials are modularly synthesized using (a) bis-substituted Ru2+ or Os2+ precursors (R2bpy)2ML2, (b) substituted 2,4-pentanediones or hydroxamic acids bearing a functionalized linker, and (c) modified nucleotides. DNA polymerase efficiently incorporates the metal-containing nucleotide triphosphate into DNA oligonucleotides.
Weizman
Haim
H
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
Tor
Yitzhak
Y
D015082:2,2'-Dipyridyl / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D004563:Electrochemistry; D009711:Nucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D012428:Ruthenium / Q000737:chemistry*; D013942:Thymine Nucleotides / Q000138:chemical synthesis / Q000737:chemistry
D009711:Nucleotides; D009942:Organometallic Compounds; D013942:Thymine Nucleotides; D009992:Osmium; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C024157:thymidine 5'-triphosphate