Effect of different solvents and crosslinkers on cytocompatibility of Type II collagen scaffolds for chondrocyte seeding.
Type II collagen extracted from porcine costal cartilage was evaluated as scaffolds for cartilage tissue engineering. Chemical crosslinkers were employed to improve the mechanical properties and the resistance toward degradation. Films and porous scaffolds were prepared from collagen solutions dissolved in 3% acetic acid (designated A) or in deionized water (designated W) and crosslinked by an epoxy (designated E) or by a carbodiimide (designated C). Immortalized rat chondrocytes and rabbit chondrocytes were used to assess cytocompatibility of crosslinked collagen matrices. Cell adhesion rate onto the films made by different preparations ranked in the order of WE > or = WC > AC > or = AE. Cell proliferation ranked in the order of AC > WC > AE > or = WE. Cells maintained round morphology only on AC and WC films. In 3-D seeding, AC scaffolds also were found to be the most cytocompatible. WC scaffolds, however, had better dimensional stability. It was concluded that Type II collagen scaffolds, when prepared by using deionized water as the solvent and carbodiimide as the crosslinker, could promote chondrocyte growth and matrix production.
Tsai
Ching-Lin
CL
Department of Orthopedics, College of Medicine, National Taiwan University, Taipei, Taiwan.
Hsu Sh
Shan-hui
SH
Cheng
Wen-Ling
WL
D000818:Animals; D002448:Cell Adhesion; D019902:Chondrocytes; D003094:Collagen; D003432:Cross-Linking Reagents; D005260:Female; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D011817:Rabbits; D012997:Solvents
D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D012997:Solvents; D003094:Collagen