[The effect of dactinomycin on retinal injury induced by argon laser in mouse].
OBJECTIVE
To determine that the administration of RNA synthesis inhibitor (dactinomycin) prevents retinal photoreceptor cell injury induced by green wavelength of argon laser in SD mice.


METHODS
At various time (0, 8, 20 h) after the exposure to laser, eyes of the mice were injected intraocularly with dactinomycin in experimental eyes, and saline in control ones, and the concentration of the drug were 10 g/L, 5 g/L, 2.5 g/L. The eyes were taken at 24 h after the exposure, the slices of the retina were stained with methyl green-pyronin (MG-P), and pyknotic (violet) as well as surviving cells (green) were counted in the laser burns.


RESULTS
Intraocular administration of dactinomycin diminished the degeneration induced by argon laser, there were marked differences between the experimental group and the control group, dactinomycin showed to be more effective if administered immediately after exposure and 8 h later, after this time the higher concentration of the drug could not result in higher effect.


CONCLUSIONS
Dactinomycin can inhibit the occurrence of the laser injury, but the time selected for the administration is critical for its efficacy.
Liu
Y
Y
Department of Ophthalmology, China PLA General Hospital, Beijing 100853, China.
He
S
S
D000818:Animals; D001128:Argon / Q000009:adverse effects; D003609:Dactinomycin / Q000008:administration & dosage* / Q000627:therapeutic use; D005131:Eye Injuries / Q000188:drug therapy* / Q000209:etiology; D007834:Lasers / Q000009:adverse effects; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000528:radiation effects*; D013997:Time Factors
D019384:Nucleic Acid Synthesis Inhibitors; D003609:Dactinomycin; D001128:Argon