Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells.
OBJECTIVE
To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells.


METHODS
Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2, were tested for HPV-16 E6 subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualize the expression of E6 subgene in the cells.


RESULTS
The HPV-16 E6 subgenetic fragment was found to be present in nuclear matrix-associated DNA, E6 oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. It was not detected, however, in EC/CUHK1 cells.


CONCLUSIONS
The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.
Chen
H B
HB
Department of Histology and Embryology, Shantou University Medical College, Guangdong Province, China. chenhb@stu.edu.cn
Chen
L
L
Zhang
J K
JK
Shen
Z Y
ZY
Su
Z J
ZJ
Cheng
S B
SB
Chew
E C
EC
D034961:Antigens, Nuclear; D002277:Carcinoma / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D012097:Repressor Proteins; D014407:Tumor Cells, Cultured
D034961:Antigens, Nuclear; C068651:E6 protein, Human papillomavirus type 16; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins