High-density cell microarrays for parallel functional determinations.
Whole-genome sequencing projects have generated a wealth of gene sequences from a variety of organisms. A major challenge is to rapidly uncover gene regulatory circuits and their functional manifestations at the cellular level. Here we report the coupled fabrication of nanocraters ranging in size from 100 pL to 1.5 nL on permeable membranes for culturing cells. Using this approach, we developed bacterial and yeast cell microarrays that allowed phenotypic determinations of gene activities and drug targets on a large scale. Cell microarrays will therefore be a particularly useful tool for studying phenotypes of gene activities on a genome-wide scale.
Xu
C Wilson
CW
Molecular Pharmacology Program, Memorial Sloan-Kettering Cancer Center and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA. w-xu@ski.mskcc.org
D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology; D016680:Genome, Bacterial; D016681:Genome, Fungal; D008567:Membranes, Artificial; D009172:Mycology / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods / Q000639:trends; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development
D008567:Membranes, Artificial