title: An NMR approach to structural proteomics.
abstract: The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
authors.affiliation: Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, Canada M5G 1L7.
authors.forename: Adelinda
authors.initials: A
authors.lastname: Yee
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chang
authors.forename: Antonio
authors.initials: A
authors.lastname: Pineda-Lucena
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Anthony
authors.initials: A
authors.lastname: Semesi
authors.forename: Brian
authors.initials: B
authors.lastname: Le
authors.forename: Theresa
authors.initials: T
authors.lastname: Ramelot
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Lee
authors.forename: Sudeepa
authors.initials: S
authors.lastname: Bhattacharyya
authors.forename: Pablo
authors.initials: P
authors.lastname: Gutierrez
authors.forename: Aleksej
authors.initials: A
authors.lastname: Denisov
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: John R
authors.initials: JR
authors.lastname: Cort
authors.forename: Guennadi
authors.initials: G
authors.lastname: Kozlov
authors.forename: Jack
authors.initials: J
authors.lastname: Liao
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Finak
authors.forename: Limin
authors.initials: L
authors.lastname: Chen
authors.forename: David
authors.initials: D
authors.lastname: Wishart
authors.forename: Weontae
authors.initials: W
authors.lastname: Lee
authors.forename: Lawrence P
authors.initials: LP
authors.lastname: McIntosh
authors.forename: Kalle
authors.initials: K
authors.lastname: Gehring
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: Aled M
authors.initials: AM
authors.lastname: Edwards
authors.forename: Cheryl H
authors.initials: CH
authors.lastname: Arrowsmith
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D019992:Databases as Topic; D004926:Escherichia coli / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D016366:Open Reading Frames; D011506:Proteins / Q000737:chemistry*; D012984:Software; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D011506:Proteins