[Investigations on Human ehrlichia infectious people in Daxingan Mountains].
OBJECTIVE
In order to investigate the prevalence of Human ehrlichia infectious people in Daxingan Mountains.


METHODS
Using PCR detection, followed by sequence analysis to detect EC and Human granulocytic ehrlichia from people in Daxingan Mountains applying the 16S rRNA gene primers of the two agents.


RESULTS
Specific 16S rRNA gene sequences of EC and HGE agent were amplified from human blood specimens of tick-bitted patients and woodsmen. The detected HGE agent 16S rRNA gene (1 433 bp) amplified from a blood specimen of a tick-bitted patient differed in only one base compared with that of an American HGE agent strain.


CONCLUSIONS
It was considered that Human ehrlichia infectious people did exist in Daxingan Mountains.
Gao
D
D
Epidemiological Department of Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Cao
W
W
Zhang
X
X
D000328:Adult; D004269:DNA, Bacterial / Q000097:blood; D004536:Ehrlichia / Q000235:genetics; D016873:Ehrlichiosis / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S