title: Follicular dendritic cells and the persistence of HIV infectivity: the role of antibodies and Fcgamma receptors.
abstract: Large quantities of HIV are found trapped on the surface of follicular dendritic cells (FDCs), and virus persists on these cells until they ultimately die. We recently found that FDCs maintain HIV infectivity for long periods in vivo and in vitro. Because FDCs trap Ags (and virus) in the form of immune complexes and are rich in FcgammaRs, we reasoned that Ab and FcgammaRs may be required for FDC-mediated maintenance of HIV infectivity. To investigate this hypothesis, HIV immune complexes were formed in vitro and incubated for increasing times with or without FDCs, after which the remaining infectious virus was determined by HIV-p24 production in rescue cultures. FDCs maintained HIV infectivity in vitro in a dose-dependent manner but required the presence of specific Ab for this activity regardless of whether laboratory-adapted or primary X4 and R5 isolates were tested. In addition, Abs against either virally or host-encoded proteins on the virion permitted FDC-mediated maintenance of HIV infectivity. We found that the addition of FDCs to HIV immune complexes at the onset of culture gave optimal maintenance of infectivity. Moreover, blocking FDC-FcgammaRs or killing the FDCs dramatically reduced their ability to preserve virus infectivity. Finally, FDCs appeared to decrease the spontaneous release of HIV-1 gp120, suggesting that FDC-virus interactions stabilize the virus particle, thus contributing to the maintenance of infectivity. Therefore, optimal maintenance of HIV infectivity requires both Ab against particle-associated determinants and FDC-FcgammaRs.
authors.affiliation: Department of Microbiology/Immunology and Anatomy, Division of Immunobiology, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA.
authors.forename: Beverly A
authors.initials: BA
authors.lastname: Smith-Franklin
authors.forename: Brandon F
authors.initials: BF
authors.lastname: Keele
authors.forename: John G
authors.initials: JG
authors.lastname: Tew
authors.forename: Suzanne
authors.initials: S
authors.lastname: Gartner
authors.forename: Andras K
authors.initials: AK
authors.lastname: Szakal
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Estes
authors.forename: Tyler C
authors.initials: TC
authors.lastname: Thacker
authors.forename: Gregory F
authors.initials: GF
authors.lastname: Burton
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000502:physiology; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003713:Dendritic Cells / Q000276:immunology* / Q000821:virology; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D008221:Lymphoid Tissue / Q000276:immunology; D017452:Receptors, IgG / Q000502:physiology*; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D015699:HIV Envelope Protein gp120; D017452:Receptors, IgG