[Change in the dynamics of a spin probe in complement-dependent lysis of a liposome].
The mobility of phospholipid chains in membranes of liposomes consisting of egg lecitin, cholesterol, dicetylphosphate, sensitized by the lipopolysaccharide antigen F. tularensis by the action of a homologous antiserum and a rabbit complement preparation was studied using 5- and 16-doxylstearate spin probes. It was shown that, during the immune lysis of liposome membranes, changes in the dynamics of spin probes occur, which correlate with the formation of transmembrane channels and exit of the fluorescent marker from the interior of liposomes. It was found that the ratio of the intensities I1/I2 of two low-field extrema in the ESR spectrum is most sensitive to changes in the liposome membrane that are induced by immune components.
Iarkov
S P
SP
State Research Institute of Biological Engineering, State Research Center, Volokolamskoe shosse 75/1, Moscow, 123424 Russia.
Tret'iakov
S I
SI
Skopinskaia
S N
SN
Khramov
E N
EN
D000818:Animals; D003165:Complement System Proteins / Q000276:immunology*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005456:Fluorescent Dyes; D008081:Liposomes; D011817:Rabbits; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D008081:Liposomes; D013113:Spin Labels; C032363:16-nitroxystearic acid; D003165:Complement System Proteins