Decrease in cytoplasmic pH-homeostastatic activity of the alkaliphile Bacillus lentus C-125 by a cell wall defect.
Cytoplasmic pH homeostatic activities of cell wall-defective derivatives of the alkaliphile Bacillus lentus C-125 were assessed using a pH-sensitive fluorescent probe, BCECF. It was shown that the acidic cell wall components took part in maintenance of the cytoplasmic pH neutrality at alkaline pH.
Ito
Masahiro
M
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Aono
Rikizo
R
D000468:Alkalies; D001407:Bacillus / Q000378:metabolism / Q000502:physiology*; D002473:Cell Wall / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006706:Homeostasis; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000378:metabolism
D000468:Alkalies; D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein