Hyperproliferative response of a monoclonal memory CD8 T cell population is characterized by an increased frequency of clonogenic precursors.
Strong memory T cell responses result partly from the selection of Ag-specific clones during immunization. In this study, we show that a monoclonal CD8 T cell population expressing a unique TCR is heterogeneous in terms of clonogenic potential following activation under optimal conditions. More importantly, the frequency of clonogenic cells is strongly increased among Ag-experienced cells, indicating that these cells were either generated or selected during the in vivo primary response. Moreover, strong proliferative responses of primed cells result from this enhanced frequency, as proliferating naive and primed cells display the same cycling parameters, i.e., lag time and intermitotic interval. Hence, these results suggest that the clonogenic potential of individual cells is imprinted before Ag encounter and that clonogenic precursors are selected or generated following in vivo activation.
Arpin
Christophe
C
Immuno-Apoptose, Institut National de la Santé et de la Recherche Médicale, Unité 503, Centre d'Etudes et de Recherche en Virologie et Immunologie, Avenue Tony Garnier, 69365 Lyon Cedex 07, France.
Angelov
Georgi
G
Walzer
Thierry
T
Tomkowiak
Martine
M
Beloeil
Laurent
L
Marvel
Jacqueline
J
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002999:Clone Cells; D016654:Genes, RAG-1; D019672:Genes, T-Cell Receptor; D007114:Immunization; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology; D013234:Stem Cells / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014758:Viral Core Proteins / Q000276:immunology
D010446:Peptide Fragments; D014758:Viral Core Proteins; C102366:nucleoprotein (366-374), influenza virus