Comparison of the potentiometric, (31)P NMR, and zero-point titration methods of determining ionized magnesium in erythrocytes.
A simple and rapid method of determining ionized magnesium in erythrocytes using a potentiometric clinical analyzer, Microlyte 6 (Kone, Finland), was investigated. The erythrocyte cell membranes were destroyed using ultrasound. The results were compared with those obtained with the (31)P nuclear magnetic resonance spectroscopy method and the zero-point titration method using atomic absorption spectrometry. The results obtained from potentiometry and from the other methods did not differ significantly (Student t test, alpha = 0.01). Total magnesium concentration was determined using atomic absorption spectrometry.
Malon
Adam
A
Faculty of Chemistry, Warsaw University, Pasteura 1, 02093 Warsaw, Poland.
Wagner
Barbara
B
Bulska
Ewa
E
Maj-Zurawska
Magdalena
M
D000328:Adult; D002413:Cations, Divalent / Q000032:analysis; D004912:Erythrocytes / Q000737:chemistry*; D006801:Humans; D017736:Ion-Selective Electrodes; D008274:Magnesium / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010759:Phosphorus Isotopes; D011199:Potentiometry / Q000379:methods*; D017186:Titrimetry / Q000379:methods
D002413:Cations, Divalent; D010759:Phosphorus Isotopes; D008274:Magnesium