Purification and some properties of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1.
A novel alkaliphilic Nocardiopsis sp., strain TOA-1, was isolated from a tile-joint of a bathroom. Strain TOA-1 produced a variety of alkaline hydrolytic enzymes. An alkaline protease, designated NAPase, was purified and characterized. NAPase had a very high keratinolytic activity and high stability under acidic conditions.
Mitsuiki
Shinji
S
Department of Industrial Chemistry, Kyushu Sangyo University, Fukuoka, Japan. mitsuiki@ip.kyusan-u.ac.jp
Sakai
Masashi
M
Moriyama
Yasushi
Y
Goto
Masatoshi
M
Furukawa
Kensuke
K
D000192:Actinomycetales / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D002364:Caseins / Q000378:metabolism*; D004269:DNA, Bacterial; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007531:Isoflurophate / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity; D013696:Temperature; D020245:p-Chloromercuribenzoic Acid / Q000494:pharmacology
D002364:Caseins; D004269:DNA, Bacterial; D011480:Protease Inhibitors; D007531:Isoflurophate; D012967:Sodium Dodecyl Sulfate; D010664:Phenylmethylsulfonyl Fluoride; D020245:p-Chloromercuribenzoic Acid; D007633:Keratins; D004492:Edetic Acid; D012697:Serine Endopeptidases