Anticoagulant effects on plasma coenzyme Q(10) estimated by HPLC with coulometric detection.
BACKGROUND
The proportion of reduced coenzyme Q(10) (Q(10)H(2)) in total coenzyme Q(10) (TQ(10)), referred to as the Q(10)H(2):TQ(10) ratio, may be used as a possible marker of in vivo oxidative stress. However, the ranges for Q(10)H(2):TQ(10) ratio from previous reports are quite variable. Sample handling and preparation appear to have a profound effect on the stability of Q(10)H(2).


METHODS
Paired tests were used to estimate TQ(10), Q(10)H(2), oxidized coenzyme Q(10) (Q(10)), and Q(10)H(2):TQ(10) ratio in patient samples collected in vacutainers containing heparin or EDTA. Sample tubes were immediately placed on ice and promptly centrifuged. After harvesting plasma, 100 microl of plasma was extracted with 1-propanol and centrifuged. The supernatant was injected directly into a high-performance liquid chromatography (HPLC) system.


RESULTS
Significantly higher values (p=0.0015) of TQ(10), Q(10)H(2), and Q(10)H(2):TQ(10) ratio were noted in heparinized plasma as compared to EDTA plasma; Q(10) concentrations were lower in heparinized plasma. When vacutainers containing specimen were opened and kept refrigerated, the Q(10)H(2):TQ(10) ratios in heparinized samples were stable over 7 h with variation <3%. Blood Q(10)H(2) in closed heparin vacutainers kept refrigerated was stable up to 24 h.


CONCLUSIONS
Our results indicate that the heparinized plasma is superior to the EDTA plasma in all measurements for coenzyme Q(10).
Tang
Peter H
PH
Division of Pediatric Neurology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA. tangp0@chmcc.org
Miles
Michael V
MV
Steele
Paul
P
DeGrauw
Antonius
A
Chuck
Gail
G
Schroer
Laura
L
Pesce
Amadeo
A
D000925:Anticoagulants / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D001793:Blood Preservation; D002851:Chromatography, High Pressure Liquid; D003067:Coenzymes; D004492:Edetic Acid / Q000494:pharmacology; D004563:Electrochemistry; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D013048:Specimen Handling; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood*
D000925:Anticoagulants; D000975:Antioxidants; D003067:Coenzymes; D007202:Indicators and Reagents; D014451:Ubiquinone; D006493:Heparin; D004492:Edetic Acid; C024989:coenzyme Q10