title: Interferon beta-1b modulates MCP-1 expression and production in relapsing-remitting multiple sclerosis.
abstract: Monocyte chemoattractant protein-1 (MCP-1) seems to be involved in the pathogenesis of multiple sclerosis (MS). We found that in unstimulated (PHA(-)) and PHA-stimulated (PHA(+)) peripheral blood mononuclear cells (PBMC), MCP-1 and TNFalpha levels are higher in stable untreated MS patients. Interferon gamma (IFNgamma) is higher in relapsing patients in PHA(-) cultures and in stable patients in PHA(+) cultures. Chronic IFNbeta-1b treatment down-regulates TNFalpha, IFNgamma and MCP-1 production except for TNFalpha in relapsing patients. IFNbeta-1b, in vitro, increases MCP-1, TNFalpha and IFNgamma spontaneous production in all patients. Multivariate analysis suggests that MCP-1 production is dependent from clinical status and not from TNFalpha and IFNgamma production. Logistic regression analysis shows that MCP-1 production is significantly modified by treatment. Further studies are needed to clarify the role of MCP-1 in MS.
authors.affiliation: Department of Oncology and Neuroscience, Neuroimmunology Unit, University "G. d'Annunzio", Via dei Vestini, I-66013, Chieti, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Iarlori
authors.forename: Marcella
authors.initials: M
authors.lastname: Reale
authors.forename: Giovanna
authors.initials: G
authors.lastname: De Luca
authors.forename: Angelo
authors.initials: A
authors.lastname: Di Iorio
authors.forename: Claudio
authors.initials: C
authors.lastname: Feliciani
authors.forename: Antonio
authors.initials: A
authors.lastname: Tulli
authors.forename: Pio
authors.initials: P
authors.lastname: Conti
authors.forename: Domenico
authors.initials: D
authors.lastname: Gambi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lugaresi
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology*; D015999:Multivariate Analysis; D012333:RNA, Messenger / Q000032:analysis; D012008:Recurrence
chemical_list: D018932:Chemokine CCL2; D012333:RNA, Messenger; D000068576:Interferon beta-1b; D016899:Interferon-beta; D000068556:Interferon beta-1a