Proline metabolism and NAD kinase activity in greenbean plants subjected to cold-shock.
The aim of the present work was to examine the relationship between proline metabolism and NAD kinase activity in greenbeans submitted to cold-shock. For this, 15-day-old greenbean plants were subjected to a temperature of 4 degrees C (cold shock) for 180 min. Our results indicate that the plants showed foliar accumulation of proline, with the enzymes ornithine-delta-aminotransferase (OAT) and proline dehydrogenase (PDH) appearing as determinant in this accumulation under cold-shock. Also, we found a close relationship between the Ca(2+)-CaM-dependent NAD kinase activity and proline metabolism, suggesting that the adaptive responses or acclimation of plants to cold stress are preceded by increased [Ca(2+)](cyt).
Ruiz
Juan M
JM
Department of Plant Biology, Faculty of Sciences, University of Granada, 18071, Granada, Spain. jruizsaez@ucdavis.edu
Sánchez
Esteban
E
García
Pablo C
PC
López-Lefebre
Luis R
LR
Rivero
Rosa M
RM
Romero
Luis
L
D003080:Cold Temperature; D007887:Fabaceae / Q000201:enzymology / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*
D011392:Proline; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase