title: [Establishment and identification of B cell lines with PNH phenotype].
abstract: OBJECTIVE
  To establish B cell lines from patients with paroxysmal nocturnal haemoglobinuria (PNH) and normal controls.
  
  
  METHODS
  B lymphoid cells prepared from PNH patients and normal controls were infected with EB virus. Phenotype of the immortal cells was analyzed by flow cytometry. The immunomagnetic beads were used to select CD(55)(-) and CD(59)(-) B lymphoid cells, which were cloned by limiting dilution. The clonality of the cloned cells was confirmed by testing the pattern of Ig rearrangements using polymerase chain reaction (PCR).
  
  
  RESULTS
  From 2 PNH patients and 2 normal controls, CD(55)(-) and CD(59)(-) B lymphoblastoid cell lines were established which had PNH phenotype and normal phenotype, respectively.
  
  
  CONCLUSIONS
  B lymphoid cell lines from PNH patients can be obtained by EB virus infection.
authors.affiliation: Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Shao
authors.forename: T
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Hao
mesh_terms: D001402:B-Lymphocytes / Q000502:physiology*; D018958:CD55 Antigens / Q000032:analysis; D018957:CD59 Antigens / Q000032:analysis; D002460:Cell Line; D018920:Coculture Techniques; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D010641:Phenotype
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens