Preparation and catalytic performance of lipases encapsulated in sol-gel materials.
Three kinds of lipases (from Candida antarctica, Pseudomonas cepacia, and Pseudomonas fluorescens) were encapsulated in inorganic matrices by the sol-gel method in order to synthesize chiral compounds by kinetic resolution. Sol-gel lipases prepared with vinyltriethoxysilane had higher hydrolysis activity for 2-octyl acetate than those with other silane precursors: tetramethoxysilane, methyltrimethoxysilane, and propyltrimethoxysilane.
Kato
Katsuya
K
Bio-functional Ceramics Group, Ceramics Research Institute, National Institute of Advanced Industrial Science and Technology, Nagoya, Japan. katsuya-kato@aist.go.jp
Gong
Yuefa
Y
Saito
Takao
T
Yokogawa
Yoshiyuki
Y
D000085:Acetates / Q000378:metabolism; D016956:Burkholderia cepacia / Q000201:enzymology*; D002175:Candida / Q000201:enzymology*; D002384:Catalysis; D006868:Hydrolysis; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D000442:Octanols / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D012821:Silanes
D000085:Acetates; D000442:Octanols; D012821:Silanes; C071718:methyltrimethoxysilane; C061205:tetramethoxysilane; C052671:vinyltriethoxysilane; D008049:Lipase; C059676:octyl acetate