title: Reassessment of CD62L as a marker of pre-effector T cells in the tumor draining lymph nodes of head and neck cancer patients.
abstract: OBJECTIVE
  CD62L was evaluated as a determinant of human pre-effector T cells.
  
  
  METHODS
  Phenotype and cytokine secretion profiles of CD62L cells were determined based on activation status.
  
  
  RESULTS
  CD62L(Low) T cells demonstrated significantly higher secretion of interleukin (IL)-10 and interferon (IFN)-gamma than did CD62L(High) T cells. After activation, the majority of cells expressed high levels of the CD62L surface marker. Postactivation levels of IL-10 production remained elevated or unchanged. In a murine B16 melanoma model, freshly isolated CD62L(Low) tumor draining lymph nodes (TDLN) T cells showed increased secretion of IL-2 and IL-4 but not of IL-10 or IFN-gamma. The surface expression of CD62L and cytokine secretion patterns were maintained after activation with concomitant increases in IL-10.
  
  
  CONCLUSIONS
  Our results provide evidence that CD62L(Low) T cells in TDLNs of progressively growing squamous cell carcinoma of the head and neck differ phenotypically and functionally from those of mouse origin.
  
  
  CONCLUSIONS
  Characterization of this human CD62L(Low) T cell population provides initial insight regarding novel surface markers in TDLN T cells that might correlate with antitumor reactivity.
authors.affiliation: Department of Otorhinolaryngology, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, Minnesota 55905, USA. strome.scott@mayo.edu
authors.forename: Scott E
authors.initials: SE
authors.lastname: Strome
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Martin
authors.forename: Dallas
authors.initials: D
authors.lastname: Flies
authors.forename: Dianne
authors.initials: D
authors.lastname: Khurana
authors.forename: Diva
authors.initials: D
authors.lastname: Salomao
authors.forename: Koji
authors.initials: K
authors.lastname: Tamada
authors.forename: Andrei I
authors.initials: AI
authors.lastname: Chapoval
authors.forename: Jan L
authors.initials: JL
authors.lastname: Kasperbauer
authors.forename: Kerry D
authors.initials: KD
authors.lastname: Olsen
authors.forename: Lieping
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000276:immunology*; D007378:Interleukins / Q000276:immunology; D019041:L-Selectin / Q000276:immunology*; D008213:Lymphocyte Activation; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D007378:Interleukins; D019041:L-Selectin