title: [Human trabecular cell culture in vitro].
abstract: OBJECTIVE
  To search the method of human trabecular cell (HTC) cultured in vitro, which could provide the necessary condition of experimental study on primary open angle glaucoma.
  
  
  METHODS
  Organ and tissue culture were taken to get the different passages of human trabecular cells. In the meantime, human scleral fibrous cells were also cultured to contrast with HTC in morphology.
  
  
  RESULTS
  (1) It took rather shorter time to get HTC by using organ culture method than simple tissue culture. (2) The 3rd to 5th passage of HTC was the most stable growth period, at that time they could be used in experiment.
  
  
  CONCLUSIONS
  Stable growth shape and characteristic HTC could be obtained by HTC culture in vitro.
authors.affiliation: Department of Ophthalmology, First Hospital, Beijing Medical University, Beijing 100034.
authors.forename: W
authors.initials: W
authors.lastname: Dai
authors.forename: M
authors.initials: M
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D012590:Sclera / Q000166:cytology; D014129:Trabecular Meshwork / Q000166:cytology*