Efficacy of novel culture environments on the ex vivo expansion kinetics of cord blood progenitor cells.
We demonstrate the efficacy of two cytokine-rich supernatants, one from peripheral blood cell cultures stimulated with anti-CD3 and the other from monocyte-conditioned media, in enhancing the rapid (within 6 days) expansion and clonogenicity of CD34+, progenitor cells. We, thereby,increased the generation of mostly myeloid precursors able to support stem cell transplants.
Cacoullos
Nike T
NT
Gritzapis
Angelos D
AD
Tsitsilonis
Athanasios E
AE
Tsiatas
Marinos L
ML
Baxevanis
Constantin N
CN
Papamichail
Michael
M
D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D017077:Culture Media, Conditioned; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007700:Kinetics; D013234:Stem Cells / Q000166:cytology*
D017077:Culture Media, Conditioned