[Detection of Escherichia coli strains harboring pathogenicity island of Yersinia enterolitica in diarrheal patients and animals in China].
OBJECTIVE
To investigate the prevalence of Escherichia coil strains harboring high pathogenicity island of Yersinia enterocolitica in diarrhea patients, animals, food samples and resulted clinical symptoms.


METHODS
Colony hybridization, DNA dot hybridization and polymerase chain reaction (PCR) were used.


RESULTS
HPI - harboring E. coli were found in the strains isolated in various provinces of China. The isolation rates were 27.05% (436/1,612) in the strains isolated from diarrhea patients, 10.23% (9/88) in the strains isolated from food samples and 5.71% (16/280) in the strains isolated from feces of cattle and pigs. Typical clinical symptoms of diarrhea caused by HPI - harboring E, coli were inappetency, abdominal pain, shiver and vapidity. Patient's temperature was usually normal or low. Over six episodes of diarrhea was frequently observed, of which most were unformed stools with mucous.


CONCLUSIONS
E. coli strains harboring high - pathogenicity - island of Yersinia species were frequently isolated from diarrhea patients, animals and food samples as well, which might be one of the major causes of diarrhea in China.
Cheng
B
B
Priority Laboratory of Molecular Medical Bacteriology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China.
Cui
S
S
Wen
X
X
Jiang
G
G
Li
L
L
Liu
Q
Q
Zhao
B
B
Ye
C
C
Xu
J
J
D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology*; D006801:Humans; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D016133:Polymerase Chain Reaction; D015008:Yersinia enterocolitica / Q000472:pathogenicity*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins