title: [Classification of genotyping hepatitis B virus with multiplex PCR].
abstract: OBJECTIVE
  To establish a simple and practicable method to identify the different genotypes of hepatitis B virus (HBV).
  
  
  METHODS
  Based on the alignment of 114 complete nucleotide sequence for HBV DNA of different genotypes, the specific sequence of each genotype was found. Six primer sets were designed for each of the six genotypes according to the genotype-specific sequence, and used separately for PCR. The genotype of HBV was identified according to the positive result of PCR. Three primer sets for B, C and D genotypes were added into a single tube for PCR reaction, and HBV was genotyped according to the length of the amplified DNA.
  
  
  RESULTS
  There was no difference in the genotyping result of PCR by single or multiplex primers, which was identical to the PCR-RFLP method.
  
  
  CONCLUSIONS
  This multiplex PCR method is simple, precise, sensitive, and easy to use.
authors.affiliation: Nanfang Hospital, the First Military Medical University, Guangzhou 510515, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Kangxian
authors.initials: K
authors.lastname: Luo
authors.forename: Yabing
authors.initials: Y
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Dai
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Jinlin
authors.initials: J
authors.lastname: Hou
mesh_terms: D017931:DNA Primers; D030561:Databases, Nucleic Acid; D005838:Genotype; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment / Q000379:methods
chemical_list: D017931:DNA Primers