title: Luffangulin, a novel ribosome inactivating peptide from ridge gourd (Luffa acutangula) seeds.
abstract: A ribosome inactivating peptide, with an N-terminal sequence exhibiting pronounced similarity to that of the 6.5 kDa-arginine/glutamate-rich polypeptide from Luffa cylindrica seeds, was isolated from seeds of a closely related species, the ridge gourd Luffa acutangula. The 5.6 kDa-peptide designated luffangulin inhibited cell-free translation with an IC50 of 3.5 nM but lacked inhibitory activity toward HIV-1 reverse transcriptase. It was similar to luffaculin, the 28 kDa ribosome inactivating protein in being unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel. On CM-cellulose luffangulin and luffaculin appeared as two adjacent peaks.
authors.affiliation: Department of Microbiology, College of Biological Science, China Agricultural University, Beijing.
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D029776:Luffa / Q000737:chemistry*; D008969:Molecular Sequence Data; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*; D012639:Seeds / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D000998:Antiviral Agents; D006023:Glycoproteins; C074465:LRIP-I protein, Luffa cylindrica; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; C088243:luffaculin protein, Luffa acutangula; D054303:HIV Reverse Transcriptase; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase