Crystallization and preliminary X-ray crystallographic analysis of beta-xylosidase from Thermoanaerobacterium saccharolyticum, a thermophilic anaerobe.
beta-Xylosidases are involved in the breakdown of xylans into xylose and belong to either family 39 or 43 of the glycosyl hydrolases. At present, no structural information is available for any member of these families. beta-Xylosidase from the thermophilic anaerobe Thermoanaerobacterium saccharolyticum, a member of glycosyl hydrolase family 39, has been crystallized at 296 K using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.4 A resolution with synchrotron X-rays and belongs to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 92.75, c = 241.37 A. The asymmetric unit contains two monomers of the recombinant enzyme, giving a corresponding V(M) of 2.21 A(3)Da(-1) and a solvent content of 44.3%.
Yang
Jin Kuk
JK
National Research Laboratory of Structural Proteomics, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
Yoon
Hye-Jin
HJ
Ahn
Hyung Jun
HJ
Lee
Byung Il
BI
Kim
Hyung-Wook
HW
Laivenieks
Maris
M
Vieille
Claire
C
Zeikus
J Gregory
JG
Suh
Se Won
SW
D003013:Clostridium / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D014995:Xylosidases / Q000737:chemistry*
D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase