[Effects of cytokine gene therapy on prolonging survival time of allografted skin after scalding in a murine model].
OBJECTIVE
To explore the role of cytokine gene therapy on prolonging survival time of allografted skin after scalding in a murine model.


METHODS
Interleukin 10 (IL-10) gene was employed as therapeutic objective gene and fibroblast was as a carrier cell. Gene transcription technique was adopted to establish an experimental murine model in which fibroblast-mediated gene therapy was used to prolong allografted skin survival time after scalding. IL-10 was transferred into fibroblastocyte (NIH3T3) by reverse transcriptive virus vector. The mice were grafted with alloskin after scalding. In addition, collagen capsulized NIH3T-3-IL-10 cells were implanted intraperitoneally in the mice so as to observe its influence on allografted skin survival time and on the changes of their main internal organs.


RESULTS
Cytokine gene therapy could obviously prolong the survival time of allografted skin (P < 0.01) without any evident detrimental effect on the internal organs.


CONCLUSIONS
These results demonstrated that skin allograft rejection could be inhibited and the survival time be prolonged with the implantation of the fibroblastocyte transferred IL-10 gene.
Zheng
J
J
Department of Burn and Plastic Surgery, General Hospital of Urumqi, Urumqi 830000, China.
Gu
C
C
Lin
Z
Z
D000818:Animals; D002056:Burns / Q000601:surgery*; D015316:Genetic Therapy; D006085:Graft Survival; D016753:Interleukin-10 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D016753:Interleukin-10