title: Comparison of molecular and conventional methods for typing of enteroviral isolates.
abstract: Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.
authors.affiliation: Department of Clinical Virology, Christian Medical College and Hospital, Vellore-632004, India.
authors.forename: Darly Joseph
authors.initials: DJ
authors.lastname: Manayani
authors.forename: R V
authors.initials: RV
authors.lastname: Shaji
authors.forename: G John
authors.initials: GJ
authors.lastname: Fletcher
authors.forename: Thomas
authors.initials: T
authors.lastname: Cherian
authors.forename: N
authors.initials: N
authors.lastname: Murali
authors.forename: N
authors.initials: N
authors.lastname: Sathish
authors.forename: Tilak
authors.initials: T
authors.lastname: Solomon
authors.forename: Chandran
authors.initials: C
authors.lastname: Gnanamuthu
authors.forename: Gopalan
authors.initials: G
authors.lastname: Sridharan
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins; D004770:Enterovirus / Q000145:classification*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D036022:Capsid Proteins