title: [A gene study on multidrug resistance of retinoblastoma].
abstract: OBJECTIVE
  To investigate the expression of multidrug resistance gene (MDR1) and multidrug associated protein (MRP) gene in the retinoblastoma (Rb) cell line HXO-RB(44), and study the mechanism of multidrug resistance (MDR) phenotype occurring in retinoblastoma.
  
  
  METHODS
  Reverse transcriptase polymerase chain reaction (RT-PCR) was employed to detect the transcription of MDR1 and MRP gene in the HXO-RB(44) cell line, and immunohistochemical technique was used to detect their protein products P-glycoprotein and multidrug resistance associated protein (P190).
  
  
  RESULTS
  Both MDR1 and MRP genes were expressed in HXO-RB(44) cell line, as well as P-glycoprotein and P190 were over-expressed (96% - 97%) in this cell line.
  
  
  CONCLUSIONS
  Over-expression of MDR1 and MRP genes is significantly involved in the mechanism of MDR phenotype in retinoblastoma.
authors.affiliation: Department of Ophthalmology, Xiangya Second Hospital, Certral South University, Changsha 410011, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Gu
mesh_terms: D045744:Cell Line, Tumor; D019450:Genes, MDR / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger