A multitechnique approach in protein/surfactant interaction study: physicochemical aspects of sodium dodecyl sulfate in the presence of trypsin in aqueous medium.
Interaction of sodium dodecyl sulfate (SDS) with a globular protein, trypsin, has been physicochemically studied in aqueous medium in detail using tensiometric, conductometric, calorimetric, fluorimetric, viscometric, and circular dichroism techniques. The results indicate that SDS-trypsin aggregates start to form at a surfactant concentration higher than the critical micelle concentration of pure SDS micelle. In contrast, the counterion binding decreases in the presence of trypsin. The free energies and enthalpies of micellization, interfacial adsorption, and entropy of micellization associated with the interaction have also been calculated. The values show that the interaction phenomenon is entropy controlled and endothermic in nature. The increase in viscosity is observed for the system of SDS-trypsin cluster above the critical micelle concentration of SDS micelle only. The aggregation number and interface polarity decrease compared to the values of micelles without protein. Circular dichroism spectra show the high alpha-helical content and unfolded structure of trypsin in the presence of SDS due to strong electrostatic repulsion leading to a probable "necklace and bead" model in the case of biopolymer-surfactant complexes.
Ghosh
Soumen
S
Department of Biophysics, Bose Institute, Centenary Building, P- 1/12, C.I.T. Scheme, VII M, Kankurgachi, Calcutta-700054, India. gsoumen70@hotmail.com
Banerjee
Asok
A
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D002151:Calorimetry; D002417:Cattle; D002942:Circular Dichroism; D003217:Conductometry; D005470:Fluorometry; D007700:Kinetics; D008823:Micelles; D010179:Pancreas / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry* / Q000378:metabolism; D013500:Surface Tension; D014357:Trypsin / Q000737:chemistry* / Q000378:metabolism
D008823:Micelles; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin