title: Neutrophil free oxygen radical production and blood total antioxidant capacity in patients with coronary heart disease using various medications.
abstract: Despite convincing results of studies in vitro, less is known about the effects of antioxidants on in vivo redox balance in humans. We developed a novel parameter of in vivo redox balance, and studied it and its relation to dental infections in 51 patients on medication for coronary heart disease (CHD) and 39 random controls matched for age group, sex, social class and locality. In vivo redox balance was the ratio of plasma antioxidant capacity, as measured with radical-trapping assay, to neutrophil respiratory burst capacity, as measured with whole blood chemiluminescence assay. Dental infections were quantitated with four rating scales. CHD patients had higher values than controls. Patients on acetosalicylic acid (ASA), diuretics or beta blockers, but not the ones on calcium channel blocker, had significantly higher redox balance than non-users. Combination of calcium channel blockers and ASA was associated with redox balance similar to taking beta blockers or diuretics. Diuretics and ASA were independent determinants of redox balance in multivariate analyses. Redox balance did not correlate with severity of dental infections (Spearman's r 0.06 to 0.11). The results contrast experimental data indicating that calcium channel blockers are as antioxidants superior to other cardiovascular drugs. Total antioxidant capacity in parallel with oxygen species production capacity should be considered in attempts to solve the antioxidant paradox.
authors.affiliation: Division of Infectious Diseases, Helsinki University Central Hospital, Institute of Dentistry, University of Helsinki, Finland. kimmoma2@saunalahti.fi
authors.forename: K
authors.initials: K
authors.lastname: Mattila
authors.forename: M
authors.initials: M
authors.lastname: Ristola
authors.forename: H
authors.initials: H
authors.lastname: Repo
authors.forename: S
authors.initials: S
authors.lastname: Asikainen
authors.forename: T
authors.initials: T
authors.lastname: Metsä-Ketelä
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000627:therapeutic use; D000368:Aged; D000975:Antioxidants / Q000032:analysis*; D001241:Aspirin / Q000627:therapeutic use; D002121:Calcium Channel Blockers / Q000627:therapeutic use; D003327:Coronary Disease / Q000097:blood* / Q000188:drug therapy*; D004232:Diuretics / Q000627:therapeutic use; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000032:analysis*; D016897:Respiratory Burst
chemical_list: D000319:Adrenergic beta-Antagonists; D000975:Antioxidants; D002121:Calcium Channel Blockers; D004232:Diuretics; D017382:Reactive Oxygen Species; D001241:Aspirin