[Effects of antisense human telomerase RNA on growth of human gastric cancer cells].
OBJECTIVE
To observe the inhibitory effects of antisense human telomerase RNA (hTR) on growth of human gastric cancer cells.


METHODS
SGC7901 cell line was transfected with antisense hTR expression vector (pBBS-hTR) by lipofectAMINE. The gene transfected cells were cultured in vitro and then inoculated into nude mice.


RESULTS
Antisense hTR expression was enhanced and sense hTR expression inhibited in clones containing antisense hTR after hygromycin resistant selection which induced tumor cells apoptosis. The growth rate of the major gene transfected cells in culture and tumorigenesis of the transfected cells in nude mice were significantly reduced in comparison with those of controls. The survival time of nude mice inoculated with the gene transfected cells was markedly prolonged when compared to controls.


CONCLUSIONS
The malignant proliferation of gastric cancer cell can be reversed by antisense hTR induced cell apoptosis. Thus antisense hTR may serve as a potential therapeutic approach to the treatment of human cancer.
Zhang
F
F
Department of Gastroenterology, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
Zhang
X
X
Fan
D
D
Deng
Z
Z
Yan y
Wu
H
H
D000818:Animals; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000401:mortality / Q000473:pathology / Q000628:therapy; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology; D015996:Survival Rate; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
D016372:RNA, Antisense; D012313:RNA; D019098:Telomerase