Induction of mammalian cell death by a plant Bax inhibitor.
Arabidopsis thaliana AtBI-1 is an orthologue of mammalian Bax inhibitor-1 capable of suppressing Bax-induced cell death in yeast as well as mammalian cells. Here we investigated whether or not AtBI-1 suppresses Bax-induced cell death using human fibrosarcoma HT1080 cells. Surprisingly, AtBI-1 did not block Bax-induced cell death, but it triggered apoptotic cell death in mammalian cells. The proapoptotic effect of AtBI-1 was blocked by the X-linked caspase inhibitor XIAP, suggesting that the cell death caused by AtBI-1 is similar to that caused by Bax.
Yu
Li Hua
LH
Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-0032, Tokyo, Japan.
Kawai-Yamada
Maki
M
Naito
Mikihiko
M
Watanabe
Kaori
K
Reed
John C
JC
Uchimiya
Hirofumi
H
D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D029681:Arabidopsis Proteins / Q000235:genetics / Q000494:pharmacology*; D061945:Caspase Inhibitors; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000494:pharmacology*; D008954:Models, Biological; D011506:Proteins / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D051636:X-Linked Inhibitor of Apoptosis Protein; D051028:bcl-2-Associated X Protein
C491862:ATBI-1 protein, Arabidopsis; D051017:Apoptosis Regulatory Proteins; D029681:Arabidopsis Proteins; C494826:BAX protein, human; D061945:Caspase Inhibitors; D008565:Membrane Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; C484209:TMBIM6 protein, human; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D051028:bcl-2-Associated X Protein