title: [Anti-CD40 McAb induces CD40+ malignant B cell proliferation arrest and apoptosis].
abstract: OBJECTIVE
  To study the expression of CD(40) molecule and the biological effects mediated by CD(40) molecules on malignant B cells.
  
  
  METHODS
  Agonistic anti-human CD(40) monoclonal antibody (5C11) was added to the cell culture system. Cell counting, PI staining, Annexin-V staining and flow cytometric analysis were used to study the biological effects of 5C11 on malignant B cell lines.
  
  
  RESULTS
  5C11 induced homotypic aggregation, proliferation arrest and apoptosis in the multiple myeloma cell line XG2 highly expressed CD(40). 5C11 induced B lymphoma cell line Daudi homotypic aggregation and proliferation arrest but no apoptosis.
  
  
  CONCLUSIONS
  5C11 can inhibit proliferation of malignant B cells by inducing apoptosis or arresting the cells at G(2)/M interphase.
authors.affiliation: Department of Immunology, Suzhou Medical College, Suzhou 215007, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: X
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D017209:Apoptosis; D001402:B-Lymphocytes / Q000502:physiology*; D019013:CD40 Antigens / Q000502:physiology*; D002449:Cell Aggregation; D002455:Cell Division; D006801:Humans; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000628:therapy*; D009101:Multiple Myeloma / Q000628:therapy*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D019013:CD40 Antigens