title: Expression of Hqk encoding a KH RNA binding protein is altered in human glioma.
abstract: The quaking gene family encodes single KH domain RNA-binding proteins that play vital roles in cell differentiation, proliferation, and apoptotic processes. The human quaking gene, Hqk, maps to 6q25-q26, where cytogenetic alterations associated with a variety of human malignancies, including gliomas have been reported. To assess possible relationships of Hqk with human diseases such as glial tumors, we first isolated the Hqk gene, characterized its structure and expression pattern, and carried out mutational analysis of Hqk in primary tumor samples. The Hqk gene contains 8 exons spanning a approximately 200 kb genomic region, and generating at least four alternatively spliced transcripts, Hqk-5, Hqk-6, Hqk-7 and Hqk-7B, of which Hqk-7 is abundantly expressed in brain. Analysis of primary tumors demonstrated a high incidence of expression alterations of Hqk in gliomas (30%; 6/20), but not in other tumors such as schwannomas (0/3), or meningiomas (0/8). Among the tumor samples showing expression alterations, two were devoid of all three major transcripts, one was missing only the Hqk-5 message, and only the Hqk-7 message was absent in two cases. Our results thus imply the involvement of Hqk in human glial tumor progression.
authors.affiliation: Department of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862-0976, Japan.
authors.forename: Zheng Zhe
authors.initials: ZZ
authors.lastname: Li
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Murata
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Ebersole
authors.forename: Toru
authors.initials: T
authors.lastname: Nishi
authors.forename: Kenji
authors.initials: K
authors.lastname: Tada
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ushio
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yamamura
authors.forename: Kuniya
authors.initials: K
authors.lastname: Abe
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics*; D006801:Humans; D007438:Introns; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C490832:QKI protein, human; C098217:Qk protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins