[Application of cerium cytochemical method in observation of renal injury caused by oxygen free radical with cadmium induction].
OBJECTIVE
To study acute renal injury caused by oxygen free radical with cadmium induction.


METHODS
Electron microscopy cytochemical and energy dispersive X-ray (EDX) analysis techniques were used.


RESULTS
Cadmium ions could enter the epithelial cells of renal proximal convoluted tubules. Excessive production of oxygen free radical was found in the kidney tissues, which located mainly in the endothelial cells of blood vessels, mitochondria of epithelial cells of renal proximal convoluted tubules, microvilli and basement membrane. Swelling of epithelial cells and mitochondria and drop of epithelial cells could occur in renal proximal convoluted tubules.


CONCLUSIONS
It suggests that cadmium ions can induce the formation of large amount of oxygen free radical and cause renal injury.
Ye
X
X
Institute for Biophysics, The Second Military Medical University, Shanghai 200433.
Sha
J
J
Tang
Y
Y
Zheng
Z
Z
Yu
B
B
Yang
Y
Y
Wu
Y
Y
D058186:Acute Kidney Injury / Q000209:etiology*; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002563:Cerium; D004577:Electron Probe Microanalysis; D005609:Free Radicals; D006651:Histocytochemistry / Q000379:methods; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000473:pathology; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D051381:Rats
D005609:Free Radicals; D002104:Cadmium; D002563:Cerium; D006861:Hydrogen Peroxide; D010100:Oxygen