A study to the fibroblast-populated collagen lattic es.
OBJECTIVE: To prepare fibroblast-populated col lagen lattices quickly and economically, and to observe the shapes and distribut ion of the fibroblasts and measure FPCLs contraction successfully. METHODS: Three different volumes of FPCLs were prepared with 24 -well plates and the best volume was chosen. RESULTS: The shapes and distribution of fibroblasts of three vo lumes of FPCLs of 24-well plates were all visible clearly; the shapes of FPCLs of plate 1 were circular dish-like and their contraction was measured. Because of plate 2 with folded edge the contraction could not be measured and plate 3 w ith thickest structure was found to have no change of contraction. CONCLUSIONS: The volume (2xDMEM: 0.16 ml) of plate 1 was t he best to be chosen.
Wu
Zhiqiang
Z
Department of Oral Maxillofacial Surgery, 2nd Clinical College of China Medical University, Shenyang 110003, China.
Davies
KJ
K
Thomas
DW
D