Wax ester production from n-alkanes by Acinetobacter sp. strain M-1: ultrastructure of cellular inclusions and role of acyl coenzyme A reductase.
Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
Ishige
Takeru
T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
Tani
Akio
A
Takabe
Keiji
K
Kawasaki
Kazunori
K
Sakai
Yasuyoshi
Y
Kato
Nobuo
N
D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D017353:Gene Deletion; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D014885:Waxes / Q000378:metabolism*
D000473:Alkanes; D004952:Esters; D014885:Waxes; D000445:Aldehyde Oxidoreductases; C021351:hexadecanal dehydrogenase (acylating)