[The relationship between postburn gene expression of modulators in gut associated lymph tissue and the change in IgA plasma cells].
OBJECTIVE
To investigate the relationship between the postburn change in IgA plasma cells and those of IL- 4 and IL-6 in gut associated lymph tissue (GALT).


METHODS
One hundred and twenty -- five SPF mice were enrolled in the study and randomly divided into three groups -- normal control (A), microbial inoculation (B) and microbial inoculation followed by burn injury. (C) In groups Band C, the mice were gavaged with candida albicans, and the mice in group C, were inflicted with 20% TBSA III degree burn at the 14th day after inoculation. The mice were sacrificed thereafter on 1, 2 and 3 postburn days and the samples were obtained aseptically. The number of candida albicans adhering to intestinal mucosal membrane was counted. The number of IgA plasma cells in lamina propria was determined by immunohistochemical staining. The gene expression of IL-4 in Peyer patch was detected by dothybridization and that of IL-6 in lamina propria was determined by insitu hybridization.


RESULTS
(1) The postburn gene expression of IL-4 in Peyer patch decreased, but the IL-6 level in intestinal lamina propria increased. (2) The number of IgA plasma cells in lamina propria decreased significantly, but the adherence of candida albicans to intestinal mucous membrane increased obviously.


CONCLUSIONS
The decrement of postburn gene expression of IL-4 in Peyer patch might lead to IgA plasma cell decrease, resulting in the increase of candida albicans adhering to intestinal mucosal membrane.
Bai
X
X
Institute of Burn Research, Southwestern Hospital, Third Military Medical Vniversity, Chongqing, 400038, China.
Xiao
G
G
Tian
X
X
D000818:Animals; D002056:Burns / Q000276:immunology*; D002176:Candida albicans / Q000302:isolation & purification; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis*; D015847:Interleukin-4 / Q000235:genetics*; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics*; D007422:Intestines / Q000276:immunology*; D008221:Lymphoid Tissue / Q000378:metabolism*; D008297:Male; D051379:Mice; D010950:Plasma Cells / Q000502:physiology*
D007070:Immunoglobulin A; D015850:Interleukin-6; D015847:Interleukin-4