[Antigen loading on dendritic cells affects the cell function in stimulating T cells].
OBJECTIVE
To study the effect of antigen loading on dendritic cells (DC).


METHODS
DCs collected from peripheral blood monocytes were loaded with a tumor antigen from XG-7 cell line. These DCs were then co-cultured with allogeneic T cells and were compared with those DCs without antigen exposure.


RESULTS
Although DCs showed no change in their phenotypes after cultured with the antigen, they secreted more IL-12, and became more powerful in allogenic mixed lymphocyte reaction (MLR). Antigen-loaded DC stimulated more CD(4)(+) cells to proliferate than nonantigen-loaded DCs did. These CD(4)(+) cells did not kill XG-7 cells, but promoted CD(8)(+) cells' ability to inhibit the XG-7 proliferation.


CONCLUSIONS
The proliferation of CD(4)(+) cells after cultured with DC may become an indicator for the function of antigen loaded DC and for the efficiency of DC immunotherapy.
Wang
Y
Y
Department of Immunology, Suzhou Medical University, Suzhou 215007, China.
Xie
W
W
Qiu
Y
Y
Gu
Z
Z
Zhang
X
X
D000951:Antigens, Neoplasm / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D000951:Antigens, Neoplasm; D018664:Interleukin-12