title: Performance of industrial strains of Saccharomyces cerevisae during wine fermentation is affected by manipulation strategies based on sporulation.
abstract: Genetic manipulation of industrial wine yeast strains has become an essential tool for both the study of the molecular mechanisms underlaying their physiology and the improvement of their fermentative properties. The construction of null mutants for any gene in these usually diploid strains, by using a procedure based on sporulation of a heterozygote lacking one copy of the gene of interest, has been tested as an alternative to the tedious work of sequential disruption of the complete set of copies. Our results indicate that most of the homozygotes resulting from sporulation of wine yeast strains are defective in glucose consumption under microvinification conditions in synthetic must and produce stuck fermentations. These kinds of defects are observed even for strains derived from sporulation of wild type. Alteration of genomic features of wine strains by sporulation is responsible for these defects.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universitat de València, Spain.
authors.forename: J V
authors.initials: JV
authors.lastname: Gimren-Alcañiz
authors.forename: E
authors.initials: E
authors.lastname: Matallana
mesh_terms: D015139:Blotting, Southern; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal / Q000235:genetics / Q000378:metabolism; D014920:Wine / Q000382:microbiology*; D043324:beta-Fructofuranosidase
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C085976:MIG1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005947:Glucose