title: Use of molecular-genetically bred Coprinus cinereus strains for an efficient isolation of cellulose from rice straw.
abstract: Molecular-bred Coprinus cinereus monokaryotic strains with high lignin- and xylan-degrading activities were mixed-cultured at 27 degrees C in the liquid medium containing 0.5% (w/v) cut rice straw and 0.025% MnCl2. After 3 weeks, the culture supernatant was extensively treated with crude cellulase, showing the presence in it of 9.3% of the total cellulose of rice straw. When rice straw treated with 0.1 N NaOH or cultured with Ganoderma applanatum were used, the recoveries of the cellulose increased up to 29%. The same experiments were done by using a non-bred control strain, showing the recoveries of the cellulose from the treated or cultured rice straw to be 8%.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. mkikuchi@bio.titech.ac.jp
authors.forename: Madoka
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Ogawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Kajiwara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shishido
mesh_terms: D002482:Cellulose / Q000302:isolation & purification*; D003302:Coprinus / Q000235:genetics / Q000378:metabolism*; D012275:Oryza
chemical_list: D002482:Cellulose