title: Neuropeptide Y administration into the third ventricle does not increase sucrose or ethanol self-administration but does affect the cortical EEG and increases food intake.
abstract: BACKGROUND
  Several studies have provided indirect evidence that neuropeptide Y (NPY) may play a role in the regulation of ethanol consumption. However, the direct effects of central NPY administration on ethanol drinking are unclear.
  
  
  OBJECTIVE
  This study examined the effects of NPY on ethanol, sucrose, and food consumption as well as its concomitant effects on the cortical EEG.
  
  
  METHODS
  Wistar rats were implanted with cortical recording electrodes and a cannula in the third ventricle after using a sucrose substitution procedure to establish ethanol self-administration. NPY (0-15 microg/3.0 microl) was infused into the third ventricle prior to drinking sessions, when 10% ethanol (10E), 2% sucrose (2S), 0.5% sucrose (0.5S), or food were available. Behavior and cortical EEG were monitored during the sessions.
  
  
  RESULTS
  NPY had no effect on the intake of 10E, 2S, or 0.5S, but NPY (15 microg/3.0 microl) significantly increased food intake. Under baseline drinking conditions, EEG power in the 6-8 Hz range was significantly greater when 2S was consumed compared to 10E. NPY decreased power in the 8-16 Hz range, decreased peak frequency in the 6-8 Hz range, and increased peak frequency in the 32-50 Hz range when 10E or 2S was available.
  
  
  CONCLUSIONS
  These data suggest that NPY administration into the third ventricle preferentially regulates feeding compared to ethanol or sucrose drinking. In addition, since NPY significantly altered the cortical EEG in the absence of effects on ethanol and sucrose consumption, these data may indicate that NPY's cortical EEG effects are more related to its sedative or anxiolytic properties, rather than any effect on consumption.
authors.affiliation: The Scripps Research Institute, Department of Neuropharmacology, CVN-14, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. skatner@scripps.edu
authors.forename: S N
authors.initials: SN
authors.lastname: Katner
authors.forename: C J
authors.initials: CJ
authors.lastname: Slawecki
authors.forename: C L
authors.initials: CL
authors.lastname: Ehlers
mesh_terms: D000428:Alcohol Drinking / Q000188:drug therapy / Q000503:physiopathology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology*; D004435:Eating / Q000187:drug effects / Q000502:physiology*; D004569:Electroencephalography / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage*; D007276:Injections, Intraventricular; D008297:Male; D009478:Neuropeptide Y / Q000008:administration & dosage / Q000494:pharmacology / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012646:Self Administration; D013395:Sucrose / Q000008:administration & dosage*
chemical_list: D009478:Neuropeptide Y; D000431:Ethanol; D013395:Sucrose