[Using Hsp70 promoter to regulate target gene expression in tumor].
OBJECTIVE
To regulate gene expression in the desired tumor cells both in vitro and in vivo.


METHODS
Heat inducible green fluorescent protein (GFP) expression plasmid and adenovirus were built by using different sizes of 5' end regulating sequence from human heat shock protein (Hsp) gene as promoter and GFP cDNA as report gene. GFP expression turned on by heating was observed in the cultured cells and the tumor grown in the dorsal skin window chamber.


RESULTS
A 400 bp of Hsp gene 5' end regulating sequence can be activated by heating and it can drive report gene expression effectively both in vitro and in vivo.


CONCLUSIONS
Heating can selectively induce gene expression in the targeted tumor. It provides an useful tool for cancer gene therapy because it possibly maximizes tumor cell killing and minimizes normal tissue damage.
Huang
Q
Q
Central Laboratory, The First People's Hospital of Shanghai, Shanghai 200080, China. hangq@online.sh.cn
Li
C
C
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D011401:Promoter Regions, Genetic; D051381:Rats; D011916:Rats, Inbred F344
D018840:HSP70 Heat-Shock Proteins; D049452:Green Fluorescent Proteins