title: Rapid determination of nevirapine in human plasma by gas chromatography.
abstract: A sensitive and rapid gas chromatographic method has been developed to determine the levels of the HIV-1 non-nucleoside reverse transcriptase inhibitor nevirapine in human plasma. Quantitative recovery following liquid-liquid-extraction with diethylether from 500 microl of human plasma was achieved. Subsequently, the assay was performed with a CP-Sil 5CB capillary column, 15 m x 0.32 mm x 1.0 microm film thickness with a nitrogen-phosphorous-detector (NPD), Helium 5.0 was used as carrier gas with a constant inlet pressure of 7 p.s.i. Linear standard curves were obtained for concentrations ranging from 10 to 20 000 ng/ml. The calculated intra- and inter-day coefficients of variation were below 8%.
authors.affiliation: Department of Internal Medicine, University of Wuerzburg, Germany. p.langmann@medizin.uni-wuerzburg.de
authors.forename: Peter
authors.initials: P
authors.lastname: Langmann
authors.forename: Diana
authors.initials: D
authors.lastname: Schirmer
authors.forename: Thomas
authors.initials: T
authors.lastname: Väth
authors.forename: Steffen
authors.initials: S
authors.lastname: Desch
authors.forename: Michael
authors.initials: M
authors.lastname: Zilly
authors.forename: Hartwig
authors.initials: H
authors.lastname: Klinker
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D015658:HIV Infections / Q000097:blood; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D018894:Reverse Transcriptase Inhibitors; D019829:Nevirapine