Hydrogen peroxide regulation of bovine endothelin-converting enzyme-1.
Vascular injury leads to the production of reactive oxygen species (ROS), but the mechanisms by which ROS contribute to vascular pathology are not completely understood. We hypothesized that ROS increase endothelin converting enzyme (ECE-1) expression. We found that glucose oxidase (GO) increases ECE-1 mRNA, protein, and activity in bovine aortic endothelial cells. Catalase abolishes this effect. Glucose oxidase treatment of endothelial cells transactivates the ECE-1 promoter. The ECE-1 promoter element that mediates this response to GO is located between -444 and -216 bp. This region contains a STAT response element, and GO activates STAT-3 binding to this STAT response element. Our data suggest that STAT3 mediates hydrogen peroxide induction of ECE-1 expression.
López-Ongil
S
S
Department of Physiology and IRSIN, Alcalá University, Madrid, Spain.
Saura
M
M
Zaragoza
C
C
Gónzalez-Santiago
L
L
Rodríguez-Puyol
M
M
Lowenstein
C J
CJ
Rodríguez-Puyol
D
D
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002467:Cell Nucleus; D002478:Cells, Cultured; D003600:Cytosol; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D019332:Endothelin-1 / Q000378:metabolism; D000072396:Endothelin-Converting Enzymes; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005949:Glucose Oxidase / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008156:Luciferases / Q000378:metabolism; D008666:Metalloendopeptidases; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D050796:STAT3 Transcription Factor; D017384:Sequence Deletion; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D000975:Antioxidants; D017931:DNA Primers; D004268:DNA-Binding Proteins; D019332:Endothelin-1; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D015534:Trans-Activators; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D002374:Catalase; D008156:Luciferases; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes