title: [Inhibition of monocytes adhesion to the intima of arterial wall by local expression of antisense monocyte chemotactic protein-1].
abstract: OBJECTIVE
  To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process.
  
  
  METHODS
  Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.
  
  
  RESULTS
  Expression of antisense MCP-1 mediated by recombinant LNCX plasmid/lipsomal complex gene transfer enabled to inhibit MCP-1 gene expression and adhesion of monocyte to the intima.
  
  
  CONCLUSIONS
  MCP-1 plays an important role on the recruitment of monocytes in the arterial wall, which provides a potential clue in developing a gene therapy project for the prevention and treatment of atherogenesis.
authors.affiliation: Department of Pathology, Institute of Basic Medical Science, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100005, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Qiao
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.forename: G
authors.initials: G
authors.lastname: Ren
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: She
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology / Q000473:pathology*; D002448:Cell Adhesion; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D016373:DNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D005263:Femoral Artery / Q000648:ultrastructure*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D017539:Tunica Intima / Q000648:ultrastructure
chemical_list: D018932:Chemokine CCL2; D016373:DNA, Antisense; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein