title: The regulatory effects of whey retentate from bifidobacteria fermented milk on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME).
abstract: OBJECTIVE
  To investigate the effects of whey retentate from Bifidobacteria fermented milk.
  
  
  RESULTS
  The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within 15 days of the treatment in the vessels representing the ileum and the large intestine. The whey retentate favoured the growth of endogenous bifidobacteria and induced a decrease in Bacteroides fragilis and in sulphite-reducing clostridia, especially Clostridium perfringens. After the administration was stopped, these populations tended to revert to their original levels, except for the streptococci and the staphylococci populations. The treatment also led to an increase in acetic acid, CH4 and CO2 production, suggesting overgrowth of some anaerobic bacteria. Ammonium, generally considered as undesirable, declined.
  
  
  CONCLUSIONS
  The whey retentate clearly altered the microbial community in the SHIME.
  
  
  CONCLUSIONS
  Whey retentate appears to exert a beneficial effect on the in vitro gastrointestinal system; these findings warrant confirmation by in vivo studies.
authors.affiliation: Laboratoire d'Ecologie Microbienne et de Microbiologie, Faculté de Pharmacie, 1 rue des Louvels, 80000-Amiens, France. aicha_tirtouil@hotmail.com
authors.forename: A T
authors.initials: AT
authors.lastname: Meddah
authors.forename: A
authors.initials: A
authors.lastname: Yazourh
authors.forename: I
authors.initials: I
authors.lastname: Desmet
authors.forename: B
authors.initials: B
authors.lastname: Risbourg
authors.forename: W
authors.initials: W
authors.lastname: Verstraete
authors.forename: M B
authors.initials: MB
authors.lastname: Romond
mesh_terms: D000818:Animals; D001419:Bacteria / Q000254:growth & development; D001644:Bifidobacterium / Q000378:metabolism*; D019149:Bioreactors; D017753:Ecosystem; D005285:Fermentation; D006801:Humans; D007422:Intestines / Q000187:drug effects* / Q000382:microbiology*; D008892:Milk / Q000378:metabolism / Q000382:microbiology*; D008894:Milk Proteins / Q000494:pharmacology*; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins