Regulation of calcium currents by chemokines and their receptors.
We investigated the modulation of voltage dependent Ca(2+) currents by chemokine receptors in heterologous expression systems and neurons. Fractalkine, SDF-1alpha, RANTES and MDC inhibited the I(Ba) in CX3CR1-, CXCR4-, CCR5- and CCR4-expressing G1A1 cells, respectively. The I(Ba) inhibition was voltage-dependent, exhibited prepulse facilitation, and was blocked by N-ethylmaleimide and pertussis toxin pretreatment, indicating that it was mediated by Gi/Go. Some chemokines also inhibited the I(Ba) in subpopulations of dorsal root ganglion neurons and area postrema/nucleus tractus solitarius neurons. These data provide evidence that chemokines can potentially modulate neuronal signaling through the inhibition of neuronal Ca(2+) currents.
Oh
Seog Bae
SB
Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, 303 E. Chicago Avenue, Chicago, IL 60611, USA.
Endoh
Takayuki
T
Simen
Arthur A
AA
Ren
Dongjun
D
Miller
Richard J
RJ
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects*; D002460:Cell Line; D018925:Chemokines / Q000494:pharmacology*; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology; D020962:Heterotrimeric GTP-Binding Proteins / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology; D019707:Receptors, Chemokine / Q000502:physiology*
D015220:Calcium Channels; D018925:Chemokines; D011956:Receptors, Cell Surface; D019707:Receptors, Chemokine; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins