[An insoluble colored substrate for dextranase assay].
An assay of dextranase (EC 3.2.1.11) was developed by using Sephadex G-200 coupled with Remazol Brilliant Blue (RBB) as an insoluble substrate. The assay procedure included incubation of suspension of the colored substrate in buffer containing enzyme under study, removal of residual insoluble substrate, and measurement of the absorbance of supernatant fluid containing colored soluble hydrolysis products at 595 nm. The procedure was examined in the screening of dextranase-forming bacilli from the microbial collection of the Institute of Biology, Ufa Research Center, RAS.
Khalikova
E V
EV
Institute of Biology, Ufa Research Center, Russian Academy of Sciences, Ufa, 450054 Russia.
Usanov
N G
NG
D000880:Anthraquinones / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D003910:Dextranase / Q000032:analysis*; D003911:Dextrans / Q000737:chemistry; D005782:Gels; D013053:Spectrophotometry; D013379:Substrate Specificity
D000880:Anthraquinones; D004396:Coloring Agents; D003911:Dextrans; D005782:Gels; C025614:sephadex; D003910:Dextranase; C024758:Remazol Brilliant Blue R