title: Silence of chromosomal amplifications in colon cancer.
abstract: Oncogene activation by gene amplification is a major pathogenetic mechanism in human cancer. Using comparative genomic hybridization, we determined that metastatic human colon cancers commonly acquire numerous extra copies of chromosome arms 7p, 8q, 13q, and 20q. We then examined the consequence of these amplifications on gene expression using DNA microarrays. Of 55,000 transcripts profiled, 2,146 were determined to map to one of the four common colon cancer amplicons and to also be expressed in normal or malignant colon tissues. Of these, only 81 transcripts (3.8%) demonstrated a 2-fold increase over normal expression among cancers bearing the corresponding chromosomal amplification. Chromosomal amplifications are common in colon cancer metastasis, but increased expression of genes within these amplicons is rare.
authors.affiliation: Howard Hughes Medical Institute, Cleveland, Ohio 44106, USA.
authors.forename: Petra
authors.initials: P
authors.lastname: Platzer
authors.forename: Madhvi B
authors.initials: MB
authors.lastname: Upender
authors.forename: Keith
authors.initials: K
authors.lastname: Wilson
authors.forename: Joseph
authors.initials: J
authors.lastname: Willis
authors.forename: James
authors.initials: J
authors.lastname: Lutterbaugh
authors.forename: Arman
authors.initials: A
authors.lastname: Nosrati
authors.forename: James K V
authors.initials: JK
authors.lastname: Willson
authors.forename: David
authors.initials: D
authors.lastname: Mack
authors.forename: Thomas
authors.initials: T
authors.lastname: Ried
authors.forename: Sanford
authors.initials: S
authors.lastname: Markowitz
mesh_terms: D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005784:Gene Amplification; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009362:Neoplasm Metastasis; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D015854:Up-Regulation