Catalytic antibody therapy against the insecticide carbaryl.
Catalytic antibodies have been studied widely, but little is known about their applicability as therapeutic reagents in vivo. Here we report that carbaryl, a widely used broad-spectrum carbamate insecticide that is toxic to animals and humans, is hydrolyzed by polyclonal catalytic antibodies induced in vivo by a phosphate immunogen. To test the efficacy of the in vivo-induced polyclonal antibodies, we immunized mice with the phosphate immunogen and assayed their sensitivity to carbaryl by determining the ED(50) value, the dose that produces lowest-grade tremors in 50% of animals. We found that the ED(50) for immunized mice was 43% higher than that for nonimmunized mice and that this increase in ED(50) probably resulted from the hydrolysis of carbaryl by the catalytic antibodies in vivo. Our results suggest that polyclonal catalytic antibodies can be used as therapeutic reagents in vivo.
Wang
Jun
J
Department of Immunology, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA.
Han
Yunqing
Y
Liang
Shizhong
S
Wilkinson
Miles F
MF
D000818:Animals; D017151:Antibodies, Catalytic / Q000627:therapeutic use*; D000918:Antibody Specificity; D012721:Carbaryl / Q000276:immunology / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006241:Haptens / Q000276:immunology; D007306:Insecticides / Q000276:immunology / Q000378:metabolism / Q000633:toxicity*; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008956:Models, Chemical; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D014202:Tremor / Q000517:prevention & control
D017151:Antibodies, Catalytic; D006241:Haptens; D007306:Insecticides; D012721:Carbaryl