Crystallization and preliminary X-ray diffraction analysis of chloroplastic ascorbate peroxidase of tobacco plants.
The stromal ascorbate peroxidase of tobacco plants was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 4000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 76.8, c = 98.8 A. The calculated V(M) value based on a monomer in the asymmetric unit is 2.2 A(3)Da(-1). A data set was successfully collected to 1.6 A resolution from a frozen crystal using synchrotron radiation of wavelength 1.0 A at KEK-PF, Japan.
Wada
Kei
K
Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan. keiwada@biochem.osakafu-u.ac.jp
Tada
Toshiji
T
Nakamura
Yoshihiro
Y
Yabuta
Yukinori
Y
Yoshimura
Kazuya
K
Takeda
Toru
T
Shigeoka
Shigeru
S
Nishimura
Keiichiro
K
D060387:Ascorbate Peroxidases; D002736:Chloroplasts / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010544:Peroxidases / Q000737:chemistry*; D011487:Protein Conformation; D014026:Nicotiana / Q000737:chemistry / Q000201:enzymology*
D010544:Peroxidases; D060387:Ascorbate Peroxidases