Towards disposable lab-on-a-chip: poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection.
A fully disposable microanalytical device based on combination of poly(methylmethacrylate) (PMMA) capillary electrophoresis microchips and thick-film electrochemical detector strips is described. Variables influencing the separation efficiency and amperometric response, including separation voltage or detection potential are assessed and optimized. The versatility, simplicity and low-cost advantages of the new design are coupled to an attractive analytical performance, with good precision (relative standard deviation RSD = 1.68% for n = 10). Applicability for assays of mixtures of hydrazine, phenolic compounds, and catecholamines is demonstrated. Such coupling of low-cost PMMA-based microchips with thick-film electrochemical detectors holds great promise for mass production of single-use micrototal analytical systems.
Wang
Joseph
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. joewang@nmsu.edu
Pumera
Martin
M
Chatrathi
Madhu Prakash
MP
Escarpa
Alberto
A
Konrad
Renate
R
Griebel
Anja
A
Dörner
Wolfgang
W
Löwe
Holger
H
D002395:Catecholamines / Q000032:analysis; D004209:Disposable Equipment; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods / Q000592:standards; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D006834:Hydrazines / Q000032:analysis; D008832:Microchemistry / Q000295:instrumentation; D010636:Phenols / Q000032:analysis; D019904:Polymethyl Methacrylate
D002395:Catecholamines; D006834:Hydrazines; D010636:Phenols; C029424:hydrazine; D019904:Polymethyl Methacrylate