title: Relevance of renal-specific oxidoreductase in tubulogenesis during mammalian nephron development.
abstract: Renal-specific oxidoreductase (RSOR), an enzyme relevant to diabetic nephropathy, is exclusively expressed in renal tubules. Studies were initiated to determine whether, like other tubule-specific proteins, it selectively modulates tubulogenesis. Northern blot analyses revealed a approximately 1.5-kb transcript, and RSOR expression was detectable in mice embryonic kidneys at day 13, gradually increased by day 17, and extended into neo- and postnatal periods. RSOR mRNA and protein expression was confined to proximal tubules, commencing at gestational day 17 and increasing subsequently, but remained absent in glomeruli and medulla. Treatment with RSOR antisense oligodeoxynucleotide resulted in a dose-dependent dysmorphogenesis of metanephric explants harvested at gestational day 13. The explants were smaller and had expanded mesenchyme, and the population of tubules was markedly decreased. The glomeruli were unaffected, as assessed by mRNA expression of glomerular epithelial protein 1 and reactivity with wheat germ agglutinin. Antisense treatment led to a selective reduction of RSOR mRNA. Immunoprecipitation also indicated a selective translational blockade of RSOR. These findings suggest that RSOR is developmentally regulated, exhibits a distinct spatiotemporal distribution, and probably plays a role in tubulogenesis.
authors.affiliation: Department of Pathology, Northwestern University, Chicago, Illinois 60611, USA. y-kanwar@northwestern.edu
authors.forename: Yashpal S
authors.initials: YS
authors.lastname: Kanwar
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Yang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Tian
authors.forename: Sun
authors.initials: S
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.forename: Sumant
authors.initials: S
authors.lastname: Chugh
authors.forename: Satish K
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D016375:Antisense Elements (Genetics); D001345:Autoradiography; D015152:Blotting, Northern; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007678:Kidney Glomerulus / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D007684:Kidney Tubules / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D009399:Nephrons / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
chemical_list: D016375:Antisense Elements (Genetics); D012333:RNA, Messenger; D014909:Wheat Germ Agglutinins; D000449:Aldehyde Reductase; C451746:renal-specific oxidoreductase, mouse