[Determination of streptomycin and dihydrostreptomycin in meat by liquid chromatography/mass spectrometry].
A sensitive and selective method using liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) for the determination of aminoglycoside antibiotics, streptomycin and dihydrostreptomycin, in meat has been developed. The LC separation was performed on a TSK-gel Super ODS column (10 cm x 2 mm i.d.) using 5 mmol/L heptafluoro-n-butyric acid (HFBA)-acetonitrile (88:12) as the mobile phase at a flow rate of 0.18 mL/min. The positive ionization produced typical [M + H]+ molecular ions of both drugs (streptomycin m/z 582; dihydrostreptomycin m/z 584). The calibration graphs for streptomycin and dihydrostreptomycin were rectilinear from 0.25 to 25 ng with selected ion monitoring (SIM). The drugs were extracted with 1% metaphosphoric acid, and the extracts were added to 2 mL of 0.1 mol/L heptanesulfonic acid. The solution was cleaned up on a Bond Elut C18 (500 mg) cartridge. The recoveries of streptomycin and dihydrostreptomycin from swine and bovine muscle fortified at 0.2 microgram/g were 73.2-82.6%, and the detection limits were 0.01 microgram/g for both drugs.
Horie
M
M
Saitama Prefectural Institute of Public Health: 639-1, Kamiokubo, Saitama, Saitama 338-0824, Japan.
Yoshida
T
T
Kikuchi
Y
Y
Nakazawa
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013307:Streptomycin / Q000032:analysis*; D013552:Swine
D000900:Anti-Bacterial Agents; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin