Shedding of immature germ cells.
The immature germ cells (IGC) constitute the highest percentage (90%) of nonsperm cells (NSpC) in ejaculates from fertile or infertile men. The objective of this study was to evaluate IGC concentration and the IGC/(IGC + Sp) ratio, in normozoospermia and dispermia. Normozoospermia from men with proven fertility (NPF). nonproven fertility (NNPF). dispermia (D) and semen samples with excessive shedding of immature germ cells (GI 1.7 x 10(6) to 5 x 10(6) IGC/mL and GII > 5.0 x 10(6) IGC/mL) were used in this study. The mean value +2 SD for the NNPF (1.7 x 10(6)/mL) and the value proposed by WHO (5 x 10(6)/mL) were employed to define GI and GII groups. IGC concentration is statistically different in the studied groups. The IGC/Sp ratio showed a significant difference only between the NNPF and the D. When comparing semen parameters (Sp/ejaculate. grade (a) motility and morphology) there was a highly significant difference between NNPF and GI and GII: no difference was found between GI and GII. While studying 200 cases of dispermias 83% showed a high shedding of immature germ cells. The cytological study of nonsperm cells and the count and identification of the immature germ cells could be used to evaluate the dispermic disorders.
Ariagno
J
J
Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Department of Clinical Biochemistry, University Clinical Hospital José de San Martin, Argentina. jiariagno@dbc.ffyb.uba.ar
Curi
S
S
Mendeluk
G
G
Grinspon
D
D
Repetto
H
H
Chenlo
P
P
Pugliese
N
N
Sardi
M
M
Blanco
A M
AM
D004542:Ejaculation; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000503:physiopathology; D012016:Reference Values; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology*