title: Fast microwave-assisted dansylation of N-nitrosamines. Analysis by high-performance liquid chromatography with fluorescence detection.
abstract: A fast microwave-assisted dansylation procedure has been developed for the derivatization of N-nitrosamines prior to high-performance liquid chromatography determination. N-Nitrosomorpholine, N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosopyrrolidine and N-nitrosopiperidine are first denitrosated by hydrobromic acid-acetic acid to produce secondary amines, which are then quantitatively dansylated in 5 min using radiation power of 378 W and a maximum pressure of 1.4 bar inside the reactor. The reaction mixture is separated on a C18 column with acetonitrile-water (55:45, v/v) as mobile phase with fluorimetric detection at 531 nm (excitation at 339 nm). The detection limits range from 8 to 75 pg for N-nitrosomorpholine and N-nitrosodiethylamine, respectively. The method was applied to study the recoveries of N-nitrosamines in beer and their determination in cigarette smoke.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, University of La Laguna, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Cárdenes
authors.forename: Juan H
authors.initials: JH
authors.lastname: Ayala
authors.forename: Venerando
authors.initials: V
authors.lastname: González
authors.forename: Ana M
authors.initials: AM
authors.lastname: Afonso
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry*; D008872:Microwaves; D009602:Nitrosamines / Q000032:analysis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D003619:Dansyl Compounds; D009602:Nitrosamines