Inhibitory effect of extracts of Brazilian marine algae on human T-cell lymphotropic virus type 1 (HTLV-1)-induced syncytium formation in vitro.
Extracts from four species of Brazilian marine algae collected from the Rio de Janeiro State coast were screened to determine the inhibitory effect on HTLV-1-induced syncytium formation. Before performing the syncytium inhibition assay the 50% cytotoxic dose (CyD50) of the algal extracts was evaluated. The antiviral test was carried out in HeLa cells co-cultured with HTLV-I infected T-cell line (C91/PL cells) in the presence of marine algal extracts in the concentration inferior to that corresponding to the CyD50. It was observed that co-cultured cells exposed to Ulva fasciata extract showed 60.2% syncytium inhibition at a concentration of 2.5%. At 5% concentration, Sargassum vulgare and Vidalia obtusiloba extracts presented 78.8 and 76% syncytium inhibition, respectively. The best inhibitory activity was observed with Laminaria abyssalis that presented 100% syncytium inhibition at a concentration of 2.5%. This work shows that extracts of marine algae, mainly L. abyssalis extract, are able to inhibit the cell-to-cell contact essential for the spreading of the virus and could be useful to prevent the infection.
Romanos
MariaTeresaVillela
M
Departamento de Virologia do Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ, Brazil. teresa.romanos@micro.ufrj.br
Andrada-Serpa
Maria José
MJ
dos
SantosMartaGonçalvesMatos
S
Ribeiro
AnaCristinaF
A
Yoneshigue-Valentin
Yocie
Y
Costa
Sĵnia Soares
SS
Wigg
Marcia Dutra
MD
D020418:Algal Proteins / Q000494:pharmacology*; D001938:Brazil; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D003588:Cytopathogenic Effect, Viral; D016264:Dextran Sulfate / Q000494:pharmacology; D056890:Eukaryota / Q000737:chemistry*; D015726:Giant Cells / Q000187:drug effects* / Q000821:virology; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects; D015368:Human T-lymphotropic virus 1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D020014:K562 Cells / Q000166:cytology / Q000187:drug effects; D014779:Virus Replication
D020418:Algal Proteins; D016264:Dextran Sulfate