[The influence of hTGFbeta(1) gene transfection on the proliferation of NIH 3T3 fibroblast].
OBJECTIVE
To investigate the influence of hTGFbeta(1) ene transfection on the proliferation of in vitro cultured NIH 3T3 fibroblast.


METHODS
Lipofictin mediated co-transfected method was employed to transfect hTGFbeta(1) plasmid DNA into NIH 3T3 fibroblasts of the rats. Cell growth counting method, MTT, flow cytometry (FCM) and soft agar clone forming method were adopted to examine the biology of the transfected cells.


RESULTS
(1) Fibroblast growth slowed down after hTGFbeta(1) transfection, especially on 4th to 6th days after the transfection, which was accompanied by decreased synthesis of DNA as indicated by the increase of G(1) phase percentage (from 39.9% to 66.2% P < 0.05) and the decrease of S phase percentage (from 40.2% to 26.8%, P < 0.05); (2) There was no evident change of all the phases in a cell cycle in the blank load transfection group and in control group. (3) There was a good correlation between MTT and cell counting method (co-efficient 0.992) (4) The formation rate of the soft agar clone of fibroblast decreased from 1.18% to 0.55% (P < 0.05) after hTGFbeta(1) transfection.


CONCLUSIONS
hTGFbeta(1) gene transfection could inhibit fibroblast proliferation, which might be related to the interference of TGFbeta(1) with the DNA synthesis of fibroblasts.
Jia
C
C
Center of Burns and Plastic Surgery of PLA, Xijing Hospital, Fourth Military Medical University 710032, P. R. China.
Chen
B
B
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000502:physiology*; D051379:Mice; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000502:physiology*
D016212:Transforming Growth Factor beta; D004247:DNA