Separation of human serum transferrins with different iron-binding states by high-performance liquid chromatography using a pyridinium polymer column.
Four molecular forms of transferrins with different iron-binding states were separated by HPLC using a pyridinium polymer column. The elution order was monoferric transferrin bound to the C-site, holotransferrin, apotransferrin and monoferric transferrin bound to the N-site. Human sera were also analyzed with the column, and ICP-MS combined with HPLC was used to detect iron in each peak. Transferrin peaks separated by HPLC were also confirmed by an immunological method. The percentages of iron saturation in transferrins obtained by the HPLC method were compared with the values calculated from clinical data.
Harad
Kumiko
K
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan. kumiko@gpo.kumamoto-u.ac.jp
Kuniyasu
Akihiko
A
Nakayama
Hitoshi
H
Nakayama
Morio
M
Matsunaga
Toshiro
T
Uji
Yoshinori
Y
Sugiuchi
Hiroyuki
H
Okabe
Hiroaki
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D011108:Polymers; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000302:isolation & purification* / Q000378:metabolism
D011108:Polymers; D011725:Pyridines; D014168:Transferrin; D007501:Iron