Photochemistry of benzophenone immobilized in a major groove of DNA: formation of thermally reversible interstrand cross-link.
We here report a highly site and sequence selective formation of an interstrand cross-link of (BP)U-containing oligomer duplexes. The cross-link was found spontaneously reverted to original oligomers upon heating, providing a new method for the temporary connection of two DNA strands.
Nakatani
Kazuhiko
K
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.
Yoshida
Takashi
T
Saito
Isao
I
D001577:Benzophenones / Q000737:chemistry*; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010777:Photochemistry; D013379:Substrate Specificity; D014529:Uridine / Q000737:chemistry
D001577:Benzophenones; D003432:Cross-Linking Reagents; C047723:benzophenone; D004247:DNA; D014529:Uridine