title: Diazoxide and pinacidil uncouple pyruvate-malate-induced mitochondrial respiration.
abstract: We investigated the effects of K(ATP) channel openers diazoxide and pinacidil on the respiration rate and membrane potential (deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 microM) increased the V2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and decreased the deltapsi by 2-17% and 6-55%. These effects were also similar in the respiration medium without K+. Moreover, carboxyatractyloside completely abolished diazoxide- and pinacidil-induced uncoupling, indicating a role for the mitochondrial adenine nucleotide translocase in this process.
authors.affiliation: Institute for Biomedical Research, Kaunas University of Medicine, Lithuania. bioch@kmu.lt
authors.forename: Dalia M
authors.initials: DM
authors.lastname: Kopustinskiene
authors.forename: Jovita
authors.initials: J
authors.lastname: Jovaisiene
authors.forename: Julius
authors.initials: J
authors.lastname: Liobikas
authors.forename: Adolfas
authors.initials: A
authors.lastname: Toleikis
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D003981:Diazoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects*; D020110:Pinacidil / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014475:Uncoupling Agents / Q000494:pharmacology*
chemical_list: D000959:Antihypertensive Agents; D008293:Malates; D014475:Uncoupling Agents; D020110:Pinacidil; C030298:malic acid; D019289:Pyruvic Acid; D000226:Mitochondrial ADP, ATP Translocases; D003981:Diazoxide