Novel action of lignans isolated from Hernandia nymphaeifolia on Ca2+ signaling in human neutrophils.
The effects of five lignans (epi-aschantin, epi-magnolin, epi-yangambin, deoxypodophyllotoxin, yatein) isolated from Hernandia nymphaeifolia (Presl.) Kubitzki (Hernandiaceae) on intracellular Ca2+ levels ([Ca2+]i) in human neutrophils were investigated by using fura-2 as a fluorescent probe. In both Ca2+-containing and Ca2+-free media, the lignans (50-100 microM) did not alter basal [Ca2+]i but inhibited the [Ca2+]i increase induced by platelet activating factor (PAF, 10 microM), leukotriene B4 (LTB4, 0.2 microM), and thapsigargin (1 microM) to different extents. In Ca2+-free medium, after depleting stores of Ca2+ with PAF, LTB4 or thapsigargin, addition of 3 mM Ca2+ induced Ca2+ influx. Each of the lignans (50-100 microM) caused 39-89% inhibition of PAF-induced Ca2+ influx; whereas only epi-aschantin was able to inhibit LTB4- and thapsigargin-induced Ca2+ influx by 54-79%. Together, the results suggest that in human neutrophils, these lignans did not alter basal [Ca2+]i but inhibited Ca2+ movement induced by Ca2+ mobilizing agents.
Chao
Yu-Ying
YY
Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical University, Taiwan.
Su
Warren
W
Jan
Chung-Ren
CR
Ko
Ying-Chin
YC
Chen
Jih-Jung
JJ
Cheng
Jin-Shiung
JS
Liu
Chun-Peng
CP
Lo
Yuk-Keung
YK
Chou
Kang-Ju
KJ
Lee
Kam-Chung
KC
Chen
Wei-Chung
WC
Chen
Ih-Sheng
IS
D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D006801:Humans; D007975:Leukotriene B4 / Q000494:pharmacology; D017705:Lignans / Q000494:pharmacology*; D019684:Magnoliopsida / Q000737:chemistry*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology
D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; D017705:Lignans; D010972:Platelet Activating Factor; D007975:Leukotriene B4; D019284:Thapsigargin; D002118:Calcium; D016257:Fura-2