title: Fibronectin peptides derived from two distinct regions stimulate adipocyte differentiation by preventing fibronectin matrix assembly.
abstract: Here, we show that fibronectin (FN) peptides derived from two distinct regions promote the insulin-induced adipocyte differentiation of ST-13 cells by preventing FN fibrillogenesis. ST-13 cells formed numerous FN fibrils under nonadipogenic conditions, whereas this FN fibrillogenesis was suppressed by adipose induction with insulin. The insulin-induced adipocyte differentiation was promoted by an amino-terminal 24-kDa fragment of FN, accompanied by further suppression of FN fibrillogenesis. The 24 K fragment prevented FN matrix assembly by direct incorporation into the FN matrix. Like the 24 K fragment, a peptide from the 14th type III repeat, termed FNIII14, which suppressed the integrin alpha 5 beta 1-mediated adhesion of ST-13 cells to FN, accelerated the adipocyte differentiation by preventing FN fibrillogenesis without direct incorporation into the FN matrix. FNIII14 induced the conformation change of beta1 integrins of K562 cells from active to resting, as judged by FACS analysis using a monoclonal antibody AG89 directed to an active beta1 integrin-dependent epitope. Binding of a (125)I-labeled FN fragment containing the RGD cell adhesive site to ST-13 cell surface was dissociated by FNIII14, with a concomitant binding of FNIII14 itself to the cell surface. The affinity labeling of ST-13 cells using biotinylated FNIII14 showed that FNIII14 specifically bound to a nonintegrin membrane protein with M(r) of around 50 kDa. Thus, the results indicated that prevention of FN fibrillogenesis by the 24 K Fib 1 fragment and FNIII14 caused the promotion of adipocyte differentiation of ST-13 cells and that the former was due to the direct incorporation into the FN matrix and that the latter might be interpreted by negative regulation of FN receptor alpha 5 beta 1 activity.
authors.affiliation: Department of Patho-Physiology and Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya Funagawara-Machi, Shinjuku-Ku, Tokyo 162-0826, Japan.
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Kamiya
authors.forename: Rina
authors.initials: R
authors.lastname: Kato
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Wakabayashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Tohyama
authors.forename: Isao
authors.initials: I
authors.lastname: Enami
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ueki
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yajima
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Ishii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Katayama
authors.forename: Junichi
authors.initials: J
authors.lastname: Takagi
authors.forename: Fumio
authors.initials: F
authors.lastname: Fukai
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D005353:Fibronectins; D007328:Insulin; D010446:Peptide Fragments