Cysteine proteinases Fas1 and Fas2 are diagnostic markers for Fasciola hepatica infection in alpacas (Lama pacos).
Circulating antibody against Fasciola hepatica antigens was determined by enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis in alpacas naturally exposed to F. hepatica. Serological assay parameters were established by using sera from eight infected animals and seven controls with no record of this parasitic infection. Excretory--secretory (ES-) products, Fas1- and Fas2-ELISA were used to survey 307 alpacas from a F. hepatica endemic area in the Peruvian Andes. Seroprevalence of F. hepatica infection varied from 56.7, 64.8 and 66.8% measured by Fas1-, Fas2- and ES-ELISA, respectively. The sensitivity for ES-ELISA was 95%, corresponding Fas1- and Fas2-ELISA sensitivity values were 90 and 95%. In this population, 7% of animals were positive for F. hepatica eggs in faeces, other parasites detected were Trichuris sp. (40%), Nematodirus sp. (34.6%), Lamanema sp. (12.8%) and Eimeria sp. (11.8%). The results show that F. hepatica infected animals elicit circulating antibodies against ES, Fas1 and Fas2. Fas2-ELISA may be proposed as a sensitive assay for the immunodiagnosis of fasciolosis in alpacas.
Neyra
Victor
V
Molecular Biotechnology Unit, Laboratories for Research and Development of Science and Technology, P.O. Box 4314, 100, Lima, Peru.
Chavarry
Elizabeth
E
Espinoza
Jose R
JR
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology; D002161:Camelids, New World / Q000097:blood / Q000469:parasitology*; D003546:Cysteine Endopeptidases / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005210:Fasciola hepatica / Q000201:enzymology* / Q000276:immunology; D005211:Fascioliasis / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D005243:Feces / Q000469:parasitology; D005260:Female; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D010568:Peru / Q000453:epidemiology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003546:Cysteine Endopeptidases