title: Asian domains of four major genotypes of JC virus, Af2, B1-b, CY and SC.
abstract: JC virus (JCV) strains worldwide can be classified into various genotypes based on DNA sequence variations. To define the domains of the four major JCV genotypes in Asia, we collected urine samples at six unstudied sites: three in southeastern Asia, two in the central highlands and one in central Asia. DNA was extracted from urine samples, and used to amplify a 610-bp region of the viral genome. For each geographical site, we determined 16 to 31 sequences, from which a phylogenetic tree was constructed to unambiguously classify detected JCV isolates into distinct genotypes. From JCV genotype profiles at the sites studied here and elsewhere, the following conclusions were drawn. Although Af2 is the major genotype in Africa, this genotype also occurs in western and central Asia. B1-b mainly occurs in western and central Asia, including the central highlands. CY occurs in northeastern Asia with the southern boundary between China and southeast Asian countries. Although SC predominates in southeastern Asia, it also occurs in northern and central Asia at lower frequencies. In addition, a few minor JCV genotypes (B1-a, B2 and B3) occur at many sites. We discuss here the anthropological and medical significance of the present findings.
authors.affiliation: Department of First Anatomy, School of Dentistry, Aichi-Gakuin University Japan, Nagoya.
authors.forename: L
authors.initials: L
authors.lastname: Saruwatari
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: E
authors.initials: E
authors.lastname: Sakai
authors.forename: P
authors.initials: P
authors.lastname: Shresta
authors.forename: B K
authors.initials: BK
authors.lastname: Hoa
authors.forename: P T P
authors.initials: PT
authors.lastname: Phi
authors.forename: H P H
authors.initials: HP
authors.lastname: An
authors.forename: N T A
authors.initials: NT
authors.lastname: Tuyet
authors.forename: T
authors.initials: T
authors.lastname: Honjo
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: H Y
authors.initials: HY
authors.lastname: Zheng
authors.forename: T
authors.initials: T
authors.lastname: Takasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Yogo
mesh_terms: D000328:Adult; D001208:Asia; D004279:DNA, Viral / Q000652:urine; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007577:JC Virus / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D027601:Polyomavirus Infections / Q000821:virology*; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014412:Tumor Virus Infections / Q000821:virology
chemical_list: D004279:DNA, Viral