title: Ex vivo evaluation of human fetal membrane closure.
abstract: OBJECTIVE
  This study compares stress and leak point pressure of human fetal membranes following closure with suture, laser welding, or a new tissue sealant, SynthaSeal.
  
  
  METHODS
  Samples of fetal membrane were bisected and repaired with suture, laser welding with albumin solders, or a tissue sealant. Representative tissue samples were assessed by histology. Anastomotic strength was evaluated by tensiometry. Additionally, leak pressure was measured after membrane samples were placed onto an infusion pump/pressure transducer, incised, and repaired as above.
  
  
  RESULTS
  Membrane stress was strongest (P<0.001) for the tissue sealant group. Sutured samples were stronger than laser-welded samples. Membrane leak pressure was greatest for the tissue sealant group. The suture group demonstrated a higher leak pressure than the laser-welded group.
  
  
  CONCLUSIONS
  Human fetal membranes were not suited for repair with laser welding under the conditions tested. A new tissue sealant, SynthaSeal, may provide a simple, effective method for membrane closure following fetoscopy, amniocentesis, and open fetal surgery.
authors.affiliation: Center for Pediatric Urology and Laboratory for Minimal Invasive Urologic Surgery, Children's Hospital of New York, Weill Medical College of Cornell University, New York, New York 10021, USA.
authors.forename: Peter B
authors.initials: PB
authors.lastname: Petratos
authors.forename: Rebecca N
authors.initials: RN
authors.lastname: Baergen
authors.forename: Clifford B
authors.initials: CB
authors.lastname: Bleustein
authors.forename: Diane
authors.initials: D
authors.lastname: Felsen
authors.forename: Dix P
authors.initials: DP
authors.lastname: Poppas
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D001696:Biomechanical Phenomena; D005321:Extraembryonic Membranes / Q000502:physiology* / Q000601:surgery*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D017075:Laser Coagulation; D011247:Pregnancy; D011312:Pressure; D013314:Stress, Mechanical; D013537:Sutures; D013718:Tensile Strength; D014014:Tissue Adhesives / Q000627:therapeutic use
chemical_list: D014014:Tissue Adhesives