Nitric oxide biosensors based on Hb/phosphatidylcholine films.
Hemoglobin (Hb) was entrapped in a phosphatidylcholine (PC) film and immobilized at a pyrolytic graphite (PG) electrode surface. Its electron-transfer reactivity and enzyme activity were characterized by employing electrochemical methods. It was observed that Hb exhibited direct electrochemistry as well as enzyme-like activity towards the electrocatalytic reduction of NO in PC film. An unmediated, reagentless nitrogen oxide (NO) biosensor was accordingly prepared. Experimental results revealed that the peak current related to NO was linearly proportional to its concentration in the range of 1.0 x 10(-7)-3.0 x 10(-4) mol/L. The detection limit was estimated to be 1.0 x 10(-7) mol/L. Considering its good stability, nice selectivity and easy construction, this biosensor shows great promise for the rapid determination of traces of NO.
Fan
Chunhai
C
Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, PR China.
Pang
Jiantao
J
Shen
Pingping
P
Li
Genxi
G
Zhu
Dexu
D
D000465:Algorithms; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D004563:Electrochemistry; D004583:Electrons; D006454:Hemoglobins / Q000737:chemistry*; D008567:Membranes, Artificial; D009569:Nitric Oxide / Q000032:analysis*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry*
D006454:Hemoglobins; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D009569:Nitric Oxide