[Development of enzyme immunoassay for the herbicide chlorsulfuron].
Antibodies against the herbicide chlorsulfuron have been raised and characterized. Enzyme immunoassays (EIAs) for chlorsulfuron, involving labeled antigen or labeled antibodies, have been developed. The kinetics of antigen-antibody interactions in the EIA systems developed has been studied. Both systems exhibit equal sensitivity (1 ng/ml). The values of the coefficient of variation (CV), determined within the range of chlorsulfuron concentrations 1-100 ng/ml to be measured by the systems, are not in excess of 8%. The possibility of using glucose oxidase as a label in EIAs for chlorsulfuron has been demonstrated. Lack of cross-reactivity with a series of sulfonyl- and arylurea derivatives and triazines makes it possible to recommend the EIA systems developed for chlorsulfuron determination in the environment.
Iazynina
E V
EV
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071 Russia.
Zherdev
A V
AV
Eremin
S A
SA
Popova
V A
VA
Dzantiev
B B
BB
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000276:immunology; D003429:Cross Reactions; D005949:Glucose Oxidase; D006540:Herbicides / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000379:methods*; D011817:Rabbits; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013449:Sulfonamides; D014227:Triazines / Q000032:analysis* / Q000276:immunology
D006540:Herbicides; D013449:Sulfonamides; D014227:Triazines; D005949:Glucose Oxidase; C037137:chlorsulfuron