Generating mouse models of retinal disease using ENU mutagenesis.
We used the chemical mutagen, N-ethyl-N-nitrosourea, to induce random point mutations in the germline of the mouse strain C57BL/6 in order to generate models of retinal diseases. 1163 mutagenised first generation mice produced using this approach were examined for eye abnormalities. Approximately one-third (412) presented with some form of ocular abnormality. Most changes were unilateral and confined to the anterior segment of the eye. Less than 10% (44) of identified changes affected the posterior segment of the eye. 21 mice with varying ocular abnormalities, including 17 with retinal changes, were bred to produce second generation mice to confirm genetic inheritance. Genetic inheritance was confirmed in several of these lines including three with retinal changes.
Baird
P N
PN
University of Melbourne, Centre for Eye Research Australia (CERA), University of Melbourne, 32 Gisborne Street, East Melbourne, 3002, Victoria, Australia. pnb@unimelb.edu.au
Guymer
R H
RH
Chiu
D
D
Vincent
A L
AL
Alexander
W S
WS
Foote
S J
SJ
Hilton
D J
DJ
D000818:Animals; D001947:Breeding; D005038:Ethylnitrosourea; D005124:Eye Abnormalities / Q000235:genetics*; D005260:Female; D018095:Germ-Line Mutation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009153:Mutagens; D010641:Phenotype; D017354:Point Mutation; D012164:Retinal Diseases; D013737:Testis / Q000187:drug effects
D009153:Mutagens; D005038:Ethylnitrosourea