Participation of peroxynitrite in oxidative modification of LDL by aqueous extracts of cigarette smoke.
Aqueous extracts of cigarette smoke (CSE) can oxidatively modify plasma low-density lipoprotein (LDL). The aim of the present study was to elucidate the participation of peroxynitrite in LDL oxidation. When LDL was incubated with CSE, its oxidative modification was dependent on time and concentration. It could be effectively prevented by vitamin E, partially by superoxide dismutase, but hardly by catalase, mannitol and metal chelators. CSE also increased the 3-nitrotyrosine content in LDL. A similar increase of 3-nitrotyrosine occurred after incubation of LDL with a peroxynitrite generating agent, 3-morpholinosydnonimine, thus suggesting that prominent pro-oxidants in CSE are peroxynitrite-generating species.
Yamaguchi
Yu
Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, 663-8179, Nishinomiya, Japan. yusan@mwu.mukogowa-u.ac.jp
Kagota
Satomi
S
Haginaka
Jun
J
Kunitomo
Masaru
M
D000818:Animals; D001055:Apolipoproteins B / Q000737:chemistry / Q000187:drug effects*; D002614:Chelating Agents / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D030421:Peroxynitrous Acid / Q000494:pharmacology*; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000032:analysis*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D001055:Apolipoproteins B; D002614:Chelating Agents; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; D014028:Tobacco Smoke Pollution; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine