title: Detection of feline leukaemia virus in blood and bone marrow of cats with varying suspicion of latent infection.
abstract: The purpose of this study was to determine if polymerase chain reaction (PCR) could be used to detect FeLV proviral DNA in bone marrow samples of cats with varying suspicion of latent infection. Blood and bone marrow samples from 50 cats and bone marrow from one fetus were collected, including 16 cats with diseases suspected to be FeLV-associated. Serum enzyme-linked immunosorbent assay (ELISA), blood and bone marrow immunofluorescent antibody test (IFA), and blood and bone marrow PCR were performed on each cat, and IFA and PCR on bone marrow of the fetus. Forty-one cats were FeLV negative. Five cats and one fetus were persistently infected with FeLV. Four cats had discordant test results. No cats were positive on bone marrow PCR only. It appears persistent or latent FeLV infection is not always present in conditions classically associated with FeLV.
authors.affiliation: Department of Small Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Herring
authors.forename: G C
authors.initials: GC
authors.lastname: Troy
authors.forename: T E
authors.initials: TE
authors.lastname: Toth
authors.forename: S D
authors.initials: SD
authors.lastname: Forrester
authors.forename: L A
authors.initials: LA
authors.lastname: Weigt
authors.forename: I P
authors.initials: IP
authors.lastname: Herring
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000821:virology; D002415:Cats; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005256:Leukemia Virus, Feline / Q000235:genetics* / Q000302:isolation & purification; D016582:Leukemia, Feline / Q000175:diagnosis* / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011237:Predictive Value of Tests
chemical_list: D017931:DNA Primers; D004279:DNA, Viral