Identification and molecular characterization of a bovine G3 rotavirus which causes age-independent diarrhea in cattle.
G3 rotaviruses have been reported rarely in cattle, and none have been characterized. We report the first genomic characterization of a bovine G3 rotavirus, CP-1, which had been biologically characterized in vivo and shown to cause age-independent diarrhea. CP-1 was a G3 rotavirus as its VP7 had 92 to 96% deduced amino acid identity to those of G3 rotaviruses. However, initially, CP-1 was identified as a G10 rotavirus by RT-PCR even though the CP-1 VP7 had only 81 to 85% deduced amino acid identity to those of G10 rotaviruses. Rotavirus CP-1 was of P[5] specificity, a type common in cattle, and had a bovine NSP1 and NSP4. These results added another animal species to those in which G3 rotaviruses have been found, characterized a bovine rotavirus which caused age-independent diarrhea in calves, and raised the possibility that bovine G3 rotaviruses may be misdiagnosed as G10 rotaviruses.
El-Attar
L
L
Department of Pathology and Infectious Diseases, The Royal Veterinary College, London NW1 0TU, United Kingdom.
Dhaliwal
W
W
Iturriza-Gómara
M
M
Bridger
J C
JC
D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D003967:Diarrhea / Q000662:veterinary*; D006023:Glycoproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014118:Toxins, Biological; D017361:Viral Nonstructural Proteins / Q000737:chemistry
D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus