[Studies on the manufacture and immunogenicity of purified rabies vaccines on humans Vero cell].
OBJECTIVE
Using Vero cell as basic cultural material to improve the quality of rabies vaccines and to produce rabies vaccines for humans.


METHODS
CTN-1V10 strain were used for production. Vero cell of 150th generation were used for cultivation. Rotating cultivatal method with rotating bottle was used. Fluids with virus were collected at different time. Puritied rabies vaccine was produced on Vero cell after clarification, condensation, purification and extermination. A batch of vaccines made by this techniques were used for immunological observation. Sixty-three people were injected with this rabies vaccine according to the procedure of time of exposure. Thirty of them were injected with vaccines made in France (Verorab) while the others were injected with vaccines to be tested. Side effect and neutralizing antibody were recorded.


RESULTS
The quality of this newly developed rabies vaccines has met the quality set by WHO. After all dosages of injection, the rates of positive antibody were both 100% in two groups. The neutralizing antibody among testing group was 11.94 IU/ml comparing with control as 11.69 IU/ml.


CONCLUSIONS
Purified rabies vaccines on Vero cell for humans had reasonable manufacture technique and less little effect with good technological imnunogenicity.
Yu
W
W
Shenyang Ante Biology High-grade Science Technology Company, Shenyang 110005, China.
Liu
Z
Z
Qian
H
H
Lu
H
H
Wang
H
H
Li
F
F
Dou
Z
Z
D000818:Animals; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D006801:Humans; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology* / Q000517:prevention & control; D011819:Rabies Vaccines / Q000009:adverse effects / Q000276:immunology* / Q000592:standards; D011820:Rabies virus / Q000276:immunology*; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011819:Rabies Vaccines