title: Protective effect of Clostridium septicum alpha-toxoid vaccine against challenge with spores in guinea pigs.
abstract: The protective effect of an alpha-toxoid vaccine of Clostridium septicum purified alpha-toxin was investigated in guinea pigs. Purified alpha-toxin was treated with formalin to make toxoid, and alpha-toxoid vaccine was prepared by mixing alpha-toxoid (4 to 64 microg/dose) with an aluminum phosphate gel as adjuvant. Guinea pigs were immunized twice with different doses of alpha-toxoid vaccine, and challenged with spores of C. septicum. The guinea pigs surviving after challenge had been immunized with 8 microg/dose or more of alpha-toxoid. All these animals produced titers of 20 units or higher of antitoxin at the challenge. The results suggest that C. septicum alpha-toxin plays an important role in protection against challenge with spores in guinea pigs.
authors.affiliation: Division of Veterinary Microbiology, Kyoto Biken Laboratories, Uji, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Amimoto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Ohgitani
authors.forename: Osamu
authors.initials: O
authors.lastname: Sasaki
authors.forename: Eiji
authors.initials: E
authors.lastname: Oishi
authors.forename: Shigeji
authors.initials: S
authors.lastname: Katayama
authors.forename: Makoto
authors.initials: M
authors.lastname: Isogai
authors.forename: Sotoyuki
authors.initials: S
authors.lastname: Ota
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D003013:Clostridium / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D014121:Toxoids / Q000276:immunology* / Q000592:standards; D010738:Type C Phospholipases / Q000276:immunology; D014709:Vero Cells
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D014121:Toxoids; D010738:Type C Phospholipases