[Study of graft-versus -leukemia activity of gamma-ray irradiated haplomismatched donor lymphocytes infusion].
OBJECTIVE
To explore graft-versus-leukemia activity of 7.5 Gy gamma-ray irradiated haplomismatched donor lymphocytes infusion (DLI).


METHODS
L615 cells loaded 615 (H2(k))/BALB/c (H-2(d)) F(1) mice were infused with irradiated haplo-mismatched donor lymphocytes at 3, 5, 7 days after CTX (200 mg/kg) treatment which was given at the 3rd day after L615 cells inoculation.


RESULTS
T-cells irradiated with 7.5 Gy gamma-ray lost their proliferative capacity but preserved their cytotoxic activity against allogeneic targets. Survival of these recipient mice was longer than that of control (t = 10.03, P < 0.001) with no severe graft-versus-host disease (GVHD).


CONCLUSIONS
7.5 Gy gamma irradiation attenuated the GVHD inducing potential of allogeneic lymphocytes while preserving their graft-versus-leukemia activity. It provides a simple modality to postpone and prevent leukemia relapse.
Zhang
L
L
Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Guo
K
K
Dong
B
B
Luo
Q
Q
D000818:Animals; D005720:Gamma Rays / Q000627:therapeutic use*; D006086:Graft vs Host Disease / Q000517:prevention & control; D020544:Graft vs Leukemia Effect / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D014019:Tissue Donors