[Distribution of genotypes of alcohol dehydrogenase 2 and aldehyde dehydrogenase 2 in Japanese twin children].
OBJECTIVE
In order to prevent alcohol related deseases, this study investigated the distribution of the genes controlling alcohol metabolism in Japan's twin.


METHODS
Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) technique was used to measure the control gene of alcohol metabolized enzymes and the genotypes of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2), which were distributed in Japan's twins. At the same time, according to the difference in genotypes, the sensitive individuals were screened from the study subjects.


RESULTS
The distribution of ADH2 and ALDH2 genes were consistent with the Hardy-weinberg equation. The three genotypes of ADH2 gene were ADH2(1)/ADH2(1) (1.1%), ADH2(1)/ADH2(2) (44.6%) and ADH2(2)/ADH2(2) (54.3%). And those of ALDH2 gene were ALDH2(1)/ALDH2(1) (41.3%), ALDH2(1)/ALDH2(2) (39.1%) and ALDH2(2)/ALDH2(2) (19.6%). The frequency of ADH2 and ALDH2 genes was 0.255, 0.745 and 0.609, 0.391 respectively.


CONCLUSIONS
Not only the distribution of genotypes of ADH2 and ALDH2 is known, but also the sensitive individuals are found, which can help prevent alcohol related disease.
Qu
W
W
Department of Environmental Health, School of Public Health, Shanghai Medical University, Shanghai 200032.
Yamagata
Z
Z
Wu
D
D
Zhang
B
B
Zhang
Y
Y
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000435:Alcoholic Intoxication / Q000235:genetics / Q000517:prevention & control; D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D002648:Child; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018433:Twin Studies as Topic; D014427:Twins
D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase