Construction of low-resolution x-ray crystallographic electron density maps of the ribosome.
Advances in X-ray crystallography now allow biological macromolecules of almost any size to be imaged at atomic resolution. Here, I outline the strategy that allowed for the solution of the 70S ribosome structure to 7.8-A resolution. The most important factors involve the effective use of synchrotron radiation and the application of existing crystallographic software to very large structures.
Cate
J H
JH
Departments of Chemistry and Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA. jcate@lbl.gov
D000465:Algorithms; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D004583:Electrons; D008958:Models, Molecular; D016011:Normal Distribution; D017785:Photons; D012270:Ribosomes / Q000737:chemistry* / Q000648:ultrastructure*; D012542:Scattering, Radiation; D012984:Software