title: UbcD4, a ubiquitin-conjugating enzyme in Drosophila melanogaster expressed in pole cells.
abstract: The ability to destroy a particular protein at a particular time is central to the regulation of many cellular processes. Selective proteolysis in eukaryotic cells is carried out primarily by the ubiquitin-proteasome pathway. Attachment of a ubiquitin polymer to an unwanted protein causes it to be degraded by the proteasome. Several classes of enzyme, known as E1s, E2s and E3s, control the stepwise formation of a ubiquitin-protein conjugate. The specificity of substrate selection lies with the E2s and E3s. Here we describe the cloning of a Drosophila E2 gene, UbcD4, which is only expressed in embryos. Its expression pattern in stage 10-11 embryos suggests a role in germ cell development. UbcD4 can interact with the polyubiquitin-binding subunit of the proteasome.
authors.affiliation: Institute of Cell and Molecualr Biology, University of Edinburgh, UK.
authors.forename: M
authors.initials: M
authors.lastname: Canning
authors.forename: R
authors.initials: R
authors.lastname: Kirby
authors.forename: D
authors.initials: D
authors.lastname: Finnegan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid; D044763:Ubiquitin-Conjugating Enzymes
chemical_list: D029721:Drosophila Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; C453059:Ubc4 protein, Drosophila; D044763:Ubiquitin-Conjugating Enzymes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D008025:Ligases