title: Comprehensive gene expression analysis by transcript profiling.
abstract: After the completion of the genomic sequence of Arabidopsis thaliana, it is now a priority to identify all the genes, their patterns of expression and functions. Transcript profiling is playing a substantial role in annotating and determining gene functions, having advanced from one-gene-at-a-time methods to technologies that provide a holistic view of the genome. In this review, comprehensive transcript profiling methodologies are described, including two that are used extensively by the authors, cDNA-AFLP and cDNA microarraying. Both these technologies illustrate the requirement to integrate molecular biology, automation, LIMS and data analysis. With so much uncharted territory in the Arabidopsis genome, and the desire to tackle complex biological traits, such integrated systems will provide a rich source of data for the correlative, functional annotation of genes.
authors.affiliation: Ceres, Inc., Malibu, CA 90265, USA. jdonson@ceres-inc.com
authors.forename: Jonathan
authors.initials: J
authors.lastname: Donson
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Fang
authors.forename: Gregg
authors.initials: G
authors.lastname: Espiritu-Santo
authors.forename: Weimei
authors.initials: W
authors.lastname: Xing
authors.forename: Andres
authors.initials: A
authors.lastname: Salazar
authors.forename: Susie
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Veronica
authors.initials: V
authors.lastname: Armendarez
authors.forename: Wayne
authors.initials: W
authors.lastname: Volkmuth
mesh_terms: D020869:Gene Expression Profiling; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D010944:Plants / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D014158:Transcription, Genetic