DNA double-strand breaks in mouse kidney cells with age.
A Biojector device fitted with a CO2 cartridge was used to prepare single cell suspensions from kidneys of 12-month- (middle-aged) and 24-month-old (old) C57B1/6 mice. Microgel electrophoresis of DNA from these cells revealed a modest but significant 7.3% increase (P = 0.04) in DNA double-strand breaks in old mice. This increase is equivalent to the DNA damage induced by 0.1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys.
Singh
N P
NP
Department of Bioengineering, University of Washington, Seattle 98195, USA. narendra@u.washington.edu
Ogburn
C E
CE
Wolf
N S
NS
van Belle
G
G
Martin
G M
GM
D000375:Aging / Q000235:genetics*; D000818:Animals; D004249:DNA Damage; D007668:Kidney / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL