[Restriction endonuclease Sst 12I from a strain of Streptomyces sp. recognizing the nucleotide sequence 5'-CTGCAG-3'].
A new restriction endonuclease Sst12I belonging to the II type and recognizing the sequence 5'-CTGCAG-3' was isolated from the bacterial strain Streptomyces sp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 degrees and 55 degrees C and remains active after long-term storage.
Ushakova
T A
TA
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Oblast, 630559 Russia.
Puchkova
L I
LI
Gutorov
V V
VV
Kuvshinov
V N
VN
Repin
V I
VI
D010582:Bacteriophage lambda / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000145:classification / Q000302:isolation & purification*; D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004795:Enzyme Stability; D006868:Hydrolysis; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific