Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex in solution: the protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5' side.
Photoirradiation of 5-iodouracil-containing DNA, d(GTAAT(I)UAC)(2) with Sso7d protein, possessing significant kink in DNA in the crystal structure induces an unprecedented intrastrand H abstraction at the methyl group of T(5), together with selective photooxidations at Met29 of Sso7d. The reactivity of the deoxyuridin-5-yl radical can be explained by the crystal structure of the d(GTAATTAC)(2)-Sso7d complex, suggesting that the interaction of DNA-Sso7d in solution is substantially similar to its crystal structure.
Oyoshi
Takanori
T
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Surugadai, Kanda, Chiyoda, Tokyo 101-0062, Japan.
Wang
Andrew H-J
AH
Sugiyama
Hiroshi
H
D019843:Archaeal Proteins; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010777:Photochemistry; D011487:Protein Conformation; D012996:Solutions; D016944:Sulfolobus / Q000737:chemistry; D013941:Thymine / Q000737:chemistry* / Q000378:metabolism; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism
D019843:Archaeal Proteins; D018736:DNA Adducts; D004268:DNA-Binding Proteins; D012996:Solutions; C093228:Sso7d protein, Sulfolobus; D014498:Uracil; D006859:Hydrogen; D004247:DNA; C048747:5-iodouracil; D013941:Thymine