title: Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins.
abstract: We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.
authors.affiliation: Max Planck Institute for Molecular Genetics, Berlin, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Schmidt
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Eckhard
authors.initials: E
authors.lastname: Nordhoff
authors.forename: Johan
authors.initials: J
authors.lastname: Gobom
authors.forename: Joachim
authors.initials: J
authors.lastname: Klose
authors.forename: Harald
authors.initials: H
authors.lastname: Seitz
authors.forename: Volker
authors.initials: V
authors.lastname: Egelhofer
authors.forename: Holger
authors.initials: H
authors.lastname: Eickhoff
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Dolores J
authors.initials: DJ
authors.lastname: Cahill
mesh_terms: D001921:Brain / Q000196:embryology; D003001:Cloning, Molecular; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005333:Fetus; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D019151:Peptide Library; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D019151:Peptide Library; D011506:Proteins; D020543:Proteome; D011994:Recombinant Proteins