Urinary 19-norandrosterone purification by immunoaffinity chromatography: application to gas chromatography/combustion/isotope ratio mass spectrometric analysis.
The detection of exogenous 19-norandrosterone (19-NA) in urines was investigated by using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). 19-NA is, for the first time to our knowledge, isolated from urinary matrix by specific immunoaffinity chromatography (IAC) before analysis. The sample preparation consisted of a preliminary purification of urine by solid-phase extraction after hydrolysis by beta-glucuronidase. Unconjugated 19-NA was thus isolated by IAC and directly analysed by GC/C/IRMS. Optimisation of IAC purification was achieved and the reliability of the technique for anti-doping control is discussed.
Desroches
M C
MC
Laboratoire National de Dépistage du Dopage, 143 avenue Roger Salengro, 92290 Châtenay-Malabry, France.
Mathurin
J C
JC
Richard
Y
Y
Delahaut
P
P
de Ceaurriz
J
J
D000107:Acetylation; D002846:Chromatography, Affinity; D004962:Estranes / Q000276:immunology / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007120:Immunochemistry; D007202:Indicators and Reagents; D012997:Solvents
D004962:Estranes; D007202:Indicators and Reagents; D012997:Solvents; C036015:19-norandrosterone