Dye-ligand affinity adsorbents for enzyme purification.
Affinity chromatography is widely employed in laboratory and large-scale for the purification of biotherapeutics and diagnostics. Some of the most widely used ligands in affinity chromatography have been several reactive chlorotriazine dyes. In particular, immobilized anthraquinone dyes have found a plethora of applications in affinity chromatography because they are inexpensive, are resistant to chemical and biological degradation, are sterilizable and cleanable in situ, and are readily immobilized to generate affinity adsorbents which display high binding capacity for a broad spectrum of proteins. This article provides detailed protocols on the preparation of a dye-ligand affinity adsorbent. Also, detailed protocols for effective application of these media, emphasizing binding and elution conditions are presented.
Labrou
N E
NE
Department of Agricultural Biotechnology, The Agricultural University of Athens, Greece. lambrou@aua.gr
D000327:Adsorption; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents; D004798:Enzymes / Q000302:isolation & purification*; D006801:Humans; D008024:Ligands; D011506:Proteins / Q000302:isolation & purification*
D004396:Coloring Agents; D004798:Enzymes; D008024:Ligands; D011506:Proteins