Expression of luteinizing hormone genes in bovine conceptuses.
RT-PCR analysis demonstrated that bovine conceptuses at days 16, 23 and 30 expressed LH-beta-like and glycoprotein hormone alpha-like transcript sequences; adult kidney, liver and brain produced predominantly unspliced products. Sequencing of the LH-beta-like fragment (from conceptuses at day 30) indicated complete homology with the published sequence. In addition, ribonuclease protection assay of RNA samples from bovine conceptuses at day 30 with a bovine LH-beta probe revealed the presence of protected molecules that appeared to be full length. Northern blot analysis of total RNA from conceptuses at day 30 failed to demonstrate the presence of LH-beta or glycoprotein alpha subunit transcripts, whereas both transcripts were readily detected in adult pituitary RNA. Administration of hCG into the uterus of heifers from day 14 to day 16 of the oestrous cycle did not affect circulating progesterone concentrations, whereas the same dose increased progesterone concentrations (P < 0.05) when administered intravenously. These results indicate that the early bovine conceptus transcribes genes encoding LH-alpha and -beta subunits, but at a level unlikely to be of physiological consequence.
Otieno
C J
CJ
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
Wood
C M
CM
Tallam
S K
SK
Verrinder Gibbins
A M
AM
Walton
J S
JS
D000818:Animals; D015152:Blotting, Northern; D002417:Cattle / Q000097:blood / Q000196:embryology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D005260:Female; D005333:Fetus / Q000502:physiology*; D015870:Gene Expression; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics; D007275:Injections, Intravenous; D008163:Luminescent Measurements; D007986:Luteinizing Hormone / Q000235:genetics*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus
D006063:Chorionic Gonadotropin; D015292:Glycoprotein Hormones, alpha Subunit; D011374:Progesterone; D007986:Luteinizing Hormone