[The effect of TGF-beta1 on the expression of plasminogen activator inhibitors 1 and matrix metalloproteinases in cultured rat mesangial cells transfected with antisense-TGF-beta1 vector].
OBJECTIVE
To study the plasminogen activator inhibitors 1 (PAI-1) and matrix metalloproteinases (MMPs) expression on the cultured rat mesangial cells (MsC) transfected with antisense-TGF-beta1 vector.


METHODS
Lipofectin method was used to transfect antisense-TGF-beta1 vector into MsC, Western blot and Northern blot analysis for detecting TGF-beta1 peptides and antisense TGF-beta1 RNA level. The expression of PAI-1 and MMPs were determined by Northern blot, Western bolt and zymography assay.


RESULTS
The MsC clone (MAT1) which blocked the expression of TGF-beta1 peptides was successfully established. The MsC clone showed increased expression of MMP-2 mRNA protein and enzyme activity of MMP-2, 3, 7. However, expression of PAI-1 mRNA of MsC clone was suppressed.


CONCLUSIONS
Blocked expression of TGF-beta1 can upregulate the expression of MMP-2, 3, 7 and downregulate the expression of PAI-1 mRNA in MsC. It is possible that antisense-TGF-beta1 may be used for gene therapy in glomerular diseases.
Lin
W
W
Department of Pathology, School of Basic Medical Science, Shanghai Medical University, Shanghai 200032, China.
Zhang
N
N
Zhang
S
S
Gu
J
J
Guo
M
M
D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D005920:Glomerular Mesangium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*; D051381:Rats; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors*; D053773:Transforming Growth Factor beta1
D016376:Oligonucleotides, Antisense; D017395:Plasminogen Activator Inhibitor 1; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D020782:Matrix Metalloproteinases