title: Targeted inactivation of p53 in human cells does not result in aneuploidy.
abstract: Because p53 mutation and aneuploidy usually coexist, it has been suggested that p53 inactivation leads to aneuploidy. We have rigorously tested this hypothesis in diploid human cell lines in which p53 was experimentally inactivated by targeted homologous recombination. Cells completely deficient in p53 did not become aneuploid, although a slight tendency toward tetraploidization was observed. No increased rates of numerical or structural chromosomal instabilities were observed in the p53-deficient cells. Rates of sister chromatid exchange and homologous recombination were also unaffected by p53 status. These results show that inactivation of p53 does not, in and of itself, lead to the development of aneuploidy.
authors.affiliation: The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, Maryland 21231, USA.
authors.forename: Fred
authors.initials: F
authors.lastname: Bunz
authors.forename: Christine
authors.initials: C
authors.lastname: Fauth
authors.forename: Michael R
authors.initials: MR
authors.lastname: Speicher
authors.forename: Annie
authors.initials: A
authors.lastname: Dutriaux
authors.forename: John M
authors.initials: JM
authors.lastname: Sedivy
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Kinzler
authors.forename: Bert
authors.initials: B
authors.lastname: Vogelstein
authors.forename: Christoph
authors.initials: C
authors.lastname: Lengauer
mesh_terms: D000483:Alleles; D000782:Aneuploidy; D002872:Chromosome Deletion; D003110:Colonic Neoplasms / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D009154:Mutation; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53