Large-scale plant proteomics.
Large-scale and high throughput approaches increasingly play an essential role in the study of biological systems, which are per se highly complex. Therefore, they need to be examined by these extensive methods to receive information about the large genomic and proteomic networks. In plant biology, this purpose has a strong support through the accessability of the complete genome sequence of the model plant Arabidopsis thaliana. This brief review intends to focus on the basics and the state-of-the-art of these high-throughput technologies and their application to plant proteomics. It describes protein microarrays, the use of antibodies, 2-DE and MS methods and the yeast two hybrid system, which are emerging as the major technologies for plant proteomics.
Kersten
Birgit
B
Max Planck Institute of Molecular Genetics, Berlin, Germany. kersten@molgen.mpg.de
Bürkle
Lukas
L
Kuhn
Eckehard J
EJ
Giavalisco
Patrick
P
Konthur
Zoltan
Z
Lueking
Angelika
A
Walter
Gerald
G
Eickhoff
Holger
H
Schneider
Ulrich
U
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D023281:Genomics; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D020798:Two-Hybrid System Techniques
D020543:Proteome