Reversible CD8 expression induced by common cytokine receptor gamma chain-dependent cytokines in a cloned CD4(+) T(h)1 cell line.
T cells that are intrathymically lineage committed are believed to maintain their CD4 or CD8 co-receptor expression. Here, we investigated whether intrathymic lineage commitment involves irreversible genetic modification or whether co-receptor expression can be reprogrammed depending on external stimuli. The CD4(+) T(h)1 clone 2D6 established from splenic T cells as an IL-12-dependent line survived in culture with IL-2, IL-7 or IL-15 alone. Surprisingly, CD8 expression occurred in 2D6 cells upon replacement of IL-12 with any one of the three cytokines that stimulate the common cytokine receptor gamma chain, yielding CD4(+)CD8(+) 2D6 cells. CD8 expression declined when IL-2 was replaced with IL-12 and CD8 induction was inhibited when IL-12 was included in IL-2 or IL-7 culture. Our observations show that even a lineage-committed mature T cell can be reprogrammed for co-receptor expression in response to particular external stimuli.
Park
Cheung-Seog
CS
Department of Oncology, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Yang
Yi-Fu
YF
Zhou
Xu-Yu
XY
Toyooka
Kazuhito
K
Yashiro-Ohtani
Yumi
Y
Park
Woong-Ryeon
WR
Tomura
Michio
M
Tai
Xu-Guang
XG
Hamaoka
Toshiyuki
T
Fujiwara
Hiromi
H
D000818:Animals; D016827:CD8 Antigens / Q000096:biosynthesis* / Q000235:genetics; D002999:Clone Cells; D016207:Cytokines / Q000494:pharmacology*; D005786:Gene Expression Regulation; D053631:Interleukin Receptor Common gamma Subunit; D018664:Interleukin-12 / Q000037:antagonists & inhibitors / Q000494:pharmacology; D019409:Interleukin-15 / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism; D015847:Interleukin-4 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D020395:Receptors, Interleukin-7 / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology*
D016827:CD8 Antigens; D016207:Cytokines; C507942:Il2rg protein, mouse; D053631:Interleukin Receptor Common gamma Subunit; D019409:Interleukin-15; D007376:Interleukin-2; D015851:Interleukin-7; D020395:Receptors, Interleukin-7; D018664:Interleukin-12; D015847:Interleukin-4