[A study of Na(+)-K(+)-ATP ase activity in erythrocyte membrane from diabetic retinopathy patients].
OBJECTIVE
To study the changes of Na(+)-K(+)-ATPase activity in erythrocyte membrane and its significance.


METHODS
Na(+)-K(+)-ATPase activity in erythrocyte membrane was investigated with malachite green colorimetric analysis in 40 patients with diabetic retinopathy (DR), 20 diabetic patients with non DR (NDR), and 20 sex and age metched normal control subjects. The relations of Na(+)-K(+)-ATPase activity with content of Na(+) and K(+) erythrocyte deformability index (EDI), levels of fasting blood glucose (FBG), glycosylated hemoglobin (HbA(1)c), and the course of disease were also studied.


RESULTS
Na(+)-K(+)-ATPase activity in erythrocyte membrane was significantly decreased in diabetic patients (P < 0.01). The decrease was most significantly in patients with DR (P < 0.01). The decreased Na(+)-K(+)-ATPase activity was related to the content of Na(+) and K(+) in erythrocyte, the changes of EDI, FBG, HbA(1)c, and the course of disease (P < 0.01).


CONCLUSIONS
The decreased Na(+)-K(+)-ATPase activity in erythrocyte membrane affects the deformability of erythrocyte. It may contribute to the occurrence and development of DR.
He
J
J
Department of Ophthalmology, The Affiliated Hospital of Qingdao Medical College, Qingdao 266003.
Qiu
Y
Y
Zhou
X
X
D000328:Adult; D000368:Aged; D003930:Diabetic Retinopathy / Q000097:blood / Q000201:enzymology*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D000254:Sodium-Potassium-Exchanging ATPase