title: Expression, hormonal regulation, and cyclic variation of chemokines in the rat ovary: key determinants of the intraovarian residence of representatives of the white blood cell series.
abstract: A growing body of evidence suggests that mammalian ovulation bears similarities to local inflammatory reactions. Monocytes/macrophages, eosinophils, and neutrophils are known to infiltrate the area surrounding the dominant follicle before ovulation. Candidate local chemoattractants may include a family of small cytokines, also known as chemokines. In the present study, quantitative RT-PCR was used to initially identify and quantify the chemokines expressed in the preovulatory rat ovary. The chemokines monocyte chemotatic protein 1 (MCP-1), MCP-3, macrophage inflammatory protein 1alpha (MIP-1alpha), MIP-1beta, MIP-1gamma, regulated upon activation normal T cell expressed and secreted, eotaxin, interferon-inducible protein of 10 kDa, growth-regulated oncogene, lymphotactin, and fractalkine were all expressed in the PMSG-primed rat ovary 6 h post human CG. C10, T cell activation gene 3, exodus, exodus-2, cytokine-induced neutrophil chemoattractant-2, MIP-2, and lipopolysaccharide-induced C-X-C were not expressed in the PMSG-primed rat ovary 6 h post human CG. The cyclic variation of the ovary-positive chemokines was also evaluated throughout the course of a superovulated ovarian cycle. Significant preovulatory up-regulation relative to the untreated control state was documented for MCP-1 (18-fold), MCP-3 (12-fold), and growth-regulated oncogene (25-fold). In contrast, the preovulatory ovarian expression of eotaxin, fractalkine and regulated upon activation normal T cell expressed and secreted was not increased. These observations suggest that intraovarian chemokines may be responsible for the cyclic intraovarian residence of representatives of the white blood cell series.
authors.affiliation: Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Huntsman Cancer Institute, Salt Lake City, Utah 84112, USA.
authors.forename: Kenneth H H
authors.initials: KH
authors.lastname: Wong
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Negishi
authors.forename: Eli Y
authors.initials: EY
authors.lastname: Adashi
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D018925:Chemokines / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004967:Estrogens / Q000502:physiology*; D030762:Estrous Cycle / Q000378:metabolism*; D005260:Female; D007962:Leukocytes / Q000502:physiology*; D010053:Ovary / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013480:Superovulation / Q000502:physiology; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000502:physiology
chemical_list: D000199:Actins; D018925:Chemokines; D018076:DNA, Complementary; D004967:Estrogens