Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines.
The effects of the flavonoids from Scutellaria baicalensis Georgi (baicalein, baicalin and wogonin) in cultured human hepatoma cells (Hep G2, Hep 3B and SK-Hep1) were compared by MTT assay and flow cytometry. All three flavonoids dose-dependently decreased the cell viabilities accompanying the collapse of mitochondrial membrane potential and the depletion of glutathione content. However, the influence of baicalein, baicalin or wogonin on cell cycle progression was different. All three flavonoids resulted in prominent increase of G2/M population in Hep G2 cells, whereas an accumulation of sub G1 (hypoploid) peak in Hep 3B cells was observed. In SK-Hep1 cells, baicalein and baicalin resulted in dramatic boost in hypoploid peak, but wogonin made mainly in G1 phase accumulation. These data, together with the previous findings in other hepatoma cell lines, suggest that baicalein, baicalin and wogonin might be effective candidates for inducing apoptosis or inhibiting proliferation in various human hepatoma cell lines.
Chang
Wen-Huei
WH
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.
Chen
Ching-Hsein
CH
Lu
Fung-Jou
FJ
D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002453:Cell Cycle / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D005434:Flow Cytometry; D005978:Glutathione / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D019686:Lamiaceae; D008516:Medicine, Chinese Traditional; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D015394:Molecular Structure; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C038044:baicalin; C006680:baicalein; C022616:thiazolyl blue; D005978:Glutathione; C085514:wogonin