title: Opsonophagocytosis of fluorescent polystyrene beads coupled to Neisseria meningitidis serogroup A, C, Y, or W135 polysaccharide correlates with serum bactericidal activity.
abstract: We developed a polysaccharide-specific flow cytometric opsonophagocytic assay (OPA) for the simultaneous measurement of functional antibody to Neisseria meningitidis serogroups A, C, Y, and W135. OPA titers significantly correlated with serum bactericidal assay titers for all serogroups tested (mean r = 0.96; P < 0.001). OPA could be used in meningococcal vaccine evaluation.
authors.affiliation: Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jmartinez@cdc.gov
authors.forename: Joseph
authors.initials: J
authors.lastname: Martinez
authors.forename: Tamara
authors.initials: T
authors.lastname: Pilishvili
authors.forename: Suzanne
authors.initials: S
authors.lastname: Barnard
authors.forename: Joseph
authors.initials: J
authors.lastname: Caba
authors.forename: Willie
authors.initials: W
authors.lastname: Spear
authors.forename: Sandra
authors.initials: S
authors.lastname: Romero-Steiner
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D001770:Blood Bactericidal Activity / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D008589:Meningococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022401:Meningococcal Vaccines; D008863:Microspheres; D009345:Neisseria meningitidis / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D011137:Polystyrenes; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D005456:Fluorescent Dyes; D022401:Meningococcal Vaccines; D011137:Polystyrenes