[Studies on micronuclei induced by colchicine and cyclophosphamide using multicolor fluorescence in situ hybridization].
OBJECTIVE
To study chromosomal composition of micronuclei (MN) induced by colchicine (COL) and cyclophosphamide (CP) in mouse bone marrow erythrocytes.


METHODS
Multicolor fluorescence in situ hybridization (FISH) with centromeric and telomeric DNA probes was applied to analyze chromosomal composition of micronuclei induced by COL and CP in mouse bone marrow erythrocytes.


RESULTS
About 83.5% of COL-induced MN revealed both centromeric and telomeric signals. Of the CP-induced MN, 74.5% showed telomeric signals only.


CONCLUSIONS
Majority of COL-induced MN contain whole chromosome and that of CP-induced MN mainly contain acentric fragments. Multicolor FISH with centromeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.
Yang
M
M
Molecular Toxicology Labortory, Third Military University, Chongqing 400038, China.
Cao
J
J
D000818:Animals; D001854:Bone Marrow Cells / Q000648:ultrastructure; D002875:Chromosomes / Q000737:chemistry; D003078:Colchicine / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity*; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000737:chemistry / Q000235:genetics*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity
D009153:Mutagens; D003520:Cyclophosphamide; D003078:Colchicine