Carbon-14 biolabelling of wine polyphenols in Vitis vinifera cell suspension cultures.
14C-L-phenylalanine is incorporated into a range of polyphenolic compounds when fed to grape cell cultures. Optimisation of several parameters such as the quantity of precursor applied and the duration of metabolism led to incorporation yields of 15% and to specific activities of 875 mu Ci g(-1) in stilbenes. Purification of the products by several chromatographic steps is reported. Both trans- and cis-resveratrols were easily obtained by enzymatic hydrolysis of their corresponding glucosides, with specific activity of 1200-1400 mu Ci g(-1). The specific radioactivity obtained for all the compounds is suitable for in vivo feeding trials to trace their metabolic fate when consumed by animals and for in vitro activity mechanism studies. Indeed, these polyphenols seem to be implicated in the health benefits associated with regular and moderate wine consumption but little is known about their pharmacokinetics and cellular uptake.
Vitrac
Xavier
X
GESNIT EA 491, Université de Bordeaux 2, Faculté des Sciences Pharmaceutiques 3 ter, Place de la Victoire, 33076 Bordeaux Cedex, France.
Krisa
Stéphanie
S
Decendit
Alain
A
Vercauteren
Joseph
J
Nührich
Alain
A
Monti
Jean-Pierre
JP
Deffieux
Gérard
G
Mérillon
Jean-Michel
JM
D000872:Anthocyanins / Q000096:biosynthesis / Q000737:chemistry; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002839:Chromans / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids; D007553:Isotope Labeling / Q000379:methods*; D010636:Phenols / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013267:Stilbenes / Q000737:chemistry; D027843:Vitis / Q000737:chemistry / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D014920:Wine
D000872:Anthocyanins; D002250:Carbon Radioisotopes; D002839:Chromans; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D013267:Stilbenes