[Changes of vWF in vascular endothelial cells with acute hypoxia].
OBJECTIVE
To investigate changes of vWF in vascular endothelial cells with acute hypoxia and assess its significance in the study of vascular injury and hypercoagulability.


METHODS
vWF in vascular endothelial cells and in blood were measured by immunohistochemical staining image analysis and ELISA technique. Fibrin was displayed by phosphotungstic hematoxylin staining.


RESULTS
The amount of vWF in vascular endothelial cells decreased progressively, while in blood increased progressively with hypoxia. Fibrin prominently increased with hypoxia.


CONCLUSIONS
Acute hypoxia caused severe injury of vascular endothelial cells which resulted in release of vWF into blood from endothelial cells. The vWF amounts in blood may reflect the hypercoagulability, and may serve as a useful indicator of vascular injury and hypercoagulability.
Song
L
L
Department of Pathological Anatomy, Capital University of Medical Sciences, Beijing 100054, China.
Dong
X
X
Zhou
Y
Y
D000818:Animals; D004730:Endothelium, Vascular / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000032:analysis; D000860:Hypoxia / Q000097:blood / Q000503:physiopathology*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014841:von Willebrand Factor / Q000032:analysis*
D014841:von Willebrand Factor; D005337:Fibrin