title: The susceptibility of inbred mice to infection with Brachylaima cribbi (Digenea: Brachylaimidae).
abstract: Susceptibility to infection with Brachylaima cribbi was studied in eight strains of inbred mice (AKR, C3H/HeJ, CBA/CaH, BALB/c, DBA/2J, SJL/J, A/J, C57BL/6J) and Swiss albino outbred mice by quantifying faecal egg excretion over the period of the infection. Preliminary experiments indicated that a combination of filtration/sedimentation/diethyl ether sedimentation was the most sensitive and reliable technique for quantification of eggs in faeces. Mice were infected with 13-15 wild-type B. cribbi metacercariae from naturally infected Cernuella virgata and in a second experiment with human-derived B. cribbi from laboratory-reared Helix aspersa. In both experiments C57BL/6J mice were the most susceptible having the highest egg excretion levels and the longest duration of infection. Worm burdens were assessed at 12 wpi for the wild-type and at 9 wpi for the human-derived infections, when the majority of mice were no longer excreting eggs. The numbers of worms recovered from the small intestine were few and there were no significant differences among the inbred or outbred groups of mice. We have found that C57BL/6J mice were the most susceptible to Brachylaima cribbi infection as assessed by excretion of eggs and provide a suitable model for a laboratory life-cycle.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, Institute of Medical and Veterinary Science, The Queen Elizabeth Hospital, 28 Woodville Road, Woodville 5011, South Australia, Australia. andrew.butcher@imvs.sa.gov.au
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Butcher
authors.forename: Helen M
authors.initials: HM
authors.lastname: Palethorpe
authors.forename: David I
authors.initials: DI
authors.lastname: Grove
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D005260:Female; D006790:Host-Parasite Interactions; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010270:Parasite Egg Count; D014200:Trematoda / Q000472:pathogenicity*; D014201:Trematode Infections / Q000276:immunology / Q000469:parasitology*