title: DRB1*03 diversity and DRB3 associations in five major population groups in the United States.
abstract: One hundred sixty-one DRB1*03 positive individuals from each of five U.S. population groups (Caucasoids, African Americans, Asians/Pacific Islanders, Hispanics, and Native Americans) were randomly selected from a database of 82,979 individuals. DRB1*03 alleles were identified by polymerase chain reaction-sequence-specific oligonucleotide probe typing. A total of six DRB1*03 alleles out of 21 known alleles were detected. DRB1*03011 was the predominant DRB1*03 allele in all populations. Caucasoids were found to be the least diversified; only DRB1*03011 was observed. African Americans carried DRB1*03021 at a high frequency. This allele was observed in three other populations. DRB1*0304 was found in Asians/Pacific Islanders and DRB1*0305, DRB1*0307 and a new allele, DRB1*0316, was found in Hispanics. A subset of individuals was also typed for DRB3 alleles. DRB3*0101, DRB3*0202, and DRB3*0301 were detected and seven DRB1-DRB3 haplotypes were defined. Testing of other individuals not included in the DRB1*03 frequency study identified a variation of a common extended haplotype, A1, B8, DR3, which carries DRB1*0304 and two previously unreported DRB1*03 alleles, DRB1*0311 and *0320, are also described.
authors.affiliation: Department of Pediatrics, Georgetown University Medical Center, Washington, DC 20007, USA.
authors.forename: Ting F
authors.initials: TF
authors.lastname: Tang
authors.forename: Jiun
authors.initials: J
authors.lastname: Wang
authors.forename: Rebecca
authors.initials: R
authors.lastname: Slack
authors.forename: Yu Su
authors.initials: YS
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Uwe
authors.initials: U
authors.lastname: Heine
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ng
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hartzman
authors.forename: Carolyn
authors.initials: C
authors.lastname: Katovich Hurley
mesh_terms: D001741:Black or African American; D000483:Alleles; D001213:Asian / Q000235:genetics; D044383:Black People / Q000235:genetics; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics / Q000378:metabolism*; D059811:HLA-DRB1 Chains; D059826:HLA-DRB3 Chains; D006239:Haplotypes; D006630:Hispanic or Latino / Q000235:genetics; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D014481:United States; D044465:White People / Q000235:genetics
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D059826:HLA-DRB3 Chains