[Isolation of a highly purified capsular polysaccharide from Salmonella enterica serovar Typhi (Salmonella typhi)--Vi-antigen and its use in serological diagnosis of typhoid fever].
For use in differential diagnostics of typhoid fever, samples of the capsular polysaccharide from Salmonella enterica serovar Typhi (usually named Vi-antigen) were isolated and characterized by physicochemical and serological methods. It was shown that only the sample of Vi-antigen with the minimal (0.57%) admixture of the corresponding lipopolysaccharide (LPS) from S. typhi retained a high serological activity in the tests with monoreceptor anti-Vi sera. However, it exhibited a substantially weaker reaction with sera from normal donors and patients with acute nontyphoid salmonelloses, than Vi-antigen preparations with a higher (0.8-1.2%) LPS content. The chromatographically pure Vi-antigen was purified by triple reprecipitation with hexadecyltrimethylammonium bromide. The content of the LPS admixture in the resulting Vi-antigen samples was quantitatively determined by GC. A high purification level of the Vi-antigen from the LPS admixture allows us to hope that this preparation could serve as a basic component of the test system for the diagnostics of typhoid fever. The English version of the paper.
Tesheva
A M
AM
State Research Center Institute of Immunology, Ministry of Public Health of the Russian Federation, Kashirskoe sh. 24-2, Moscow, 115478 Russia. teshu@mail.ru
Aparin
P G
PG
L'vov
V L
VL
D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D009682:Magnetic Resonance Spectroscopy; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D012016:Reference Values; D012485:Salmonella typhi / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*; D014435:Typhoid Fever / Q000175:diagnosis* / Q000382:microbiology
D007106:Immune Sera; D011135:Polysaccharides, Bacterial; C054154:capsular polysaccharide, Salmonella