title: Carbon-14 biolabelling of wine polyphenols in Vitis vinifera cell suspension cultures.
abstract: 14C-L-phenylalanine is incorporated into a range of polyphenolic compounds when fed to grape cell cultures. Optimisation of several parameters such as the quantity of precursor applied and the duration of metabolism led to incorporation yields of 15% and to specific activities of 875 mu Ci g(-1) in stilbenes. Purification of the products by several chromatographic steps is reported. Both trans- and cis-resveratrols were easily obtained by enzymatic hydrolysis of their corresponding glucosides, with specific activity of 1200-1400 mu Ci g(-1). The specific radioactivity obtained for all the compounds is suitable for in vivo feeding trials to trace their metabolic fate when consumed by animals and for in vitro activity mechanism studies. Indeed, these polyphenols seem to be implicated in the health benefits associated with regular and moderate wine consumption but little is known about their pharmacokinetics and cellular uptake.
authors.affiliation: GESNIT EA 491, Université de Bordeaux 2, Faculté des Sciences Pharmaceutiques 3 ter, Place de la Victoire, 33076 Bordeaux Cedex, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Vitrac
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Krisa
authors.forename: Alain
authors.initials: A
authors.lastname: Decendit
authors.forename: Joseph
authors.initials: J
authors.lastname: Vercauteren
authors.forename: Alain
authors.initials: A
authors.lastname: Nührich
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Monti
authors.forename: Gérard
authors.initials: G
authors.lastname: Deffieux
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis / Q000737:chemistry; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002839:Chromans / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids; D007553:Isotope Labeling / Q000379:methods*; D010636:Phenols / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism*; D013267:Stilbenes / Q000737:chemistry; D027843:Vitis / Q000737:chemistry / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D014920:Wine
chemical_list: D000872:Anthocyanins; D002250:Carbon Radioisotopes; D002839:Chromans; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D013267:Stilbenes