title: Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells.
abstract: OBJECTIVE
  To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells.
  
  
  METHODS
  Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2, were tested for HPV-16 E6 subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualize the expression of E6 subgene in the cells.
  
  
  RESULTS
  The HPV-16 E6 subgenetic fragment was found to be present in nuclear matrix-associated DNA, E6 oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. It was not detected, however, in EC/CUHK1 cells.
  
  
  CONCLUSIONS
  The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.
authors.affiliation: Department of Histology and Embryology, Shantou University Medical College, Guangdong Province, China. chenhb@stu.edu.cn
authors.forename: H B
authors.initials: HB
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: J K
authors.initials: JK
authors.lastname: Zhang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Shen
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Su
authors.forename: S B
authors.initials: SB
authors.lastname: Cheng
authors.forename: E C
authors.initials: EC
authors.lastname: Chew
mesh_terms: D034961:Antigens, Nuclear; D002277:Carcinoma / Q000378:metabolism*; D004938:Esophageal Neoplasms / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D012097:Repressor Proteins; D014407:Tumor Cells, Cultured
chemical_list: D034961:Antigens, Nuclear; C068651:E6 protein, Human papillomavirus type 16; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins