Using nonaqueous capillary electrophoresis to analyze several quinolones in pig kidney samples.
We show the potential of nonaqueous capillary electrophoresis (NACE) for analyzing enrofloxacin (ENR), ciprofloxacin (CPR), danofloxacin (DAN), difloxacin (DIF), marbofloxacin (MAR), flumequine (FLU), and oxolinic acid (OXA) in pig kidney samples. We have studied the effects of parameters such as the composition of the organic media, the choice of electrolyte, the pH* of the background electrolyte (BGE), the addition of modifiers, and the reversal of electroosmotic flow. Separation was good with 20 mM ammonium acetate, 0.004% polycation hexadimethrine bromide (HDB), and 4% acetic acid (pH* 5.4) in methanol/acetonitrile (50:50 v/v) medium. We used a quick and simple sample preparation method, hydrochloric acid as an extractant and solid-phase extraction (SPE) with Baker C18 cartridges as the cleanup step. Recoveries for all quinolones were over 80%.
Hernández
Margarita
M
Departament de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, Tarragona, Spain. socias@quimica.urv.es
Borrull
Francesc
F
Calull
Marta
M
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis; D002138:Calibration; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods / Q000592:standards; D000077422:Enrofloxacin; D024841:Fluoroquinolones; D007668:Kidney / Q000737:chemistry*; D015394:Molecular Structure; D010093:Oxolinic Acid / Q000032:analysis; D011807:Quinolizines / Q000032:analysis; D015363:Quinolones / Q000032:analysis*; D013552:Swine
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; D015363:Quinolones; C068581:danofloxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; C047225:difloxacin; C080260:marbofloxacin; D010093:Oxolinic Acid; C012976:flumequine