[Prolonging the survival of transplanted alloskin by transferring the CTLA(4)Ig locally with recombinant adenovirus vector in mice].
OBJECTIVE
To find a simple and practical way to prolong the survival of transplanted alloskin to cover the burn and other wounds.


METHODS
A recombinant CTLA(4)Ig-Adenovirus expression vector was constructed by means of homo recombination, and then transferred either into cultured murine skin grafts ex vivo or local wound surface. The expression of CTLA(4)Ig in cultured skin was immunohistochemically determined. Moreover, the survival time of transplanted alloskin from Kunming mice to Balb/c mice was evaluated.


RESULTS
The CTLA(4)Ig-Adenovirus vector could be successfully transferred and expressed in the cultured murine skin, and the locally used recombinant CTLA(4)Ig-Adenovirus vector could significantly prolong the survival of the transplanted alloskin from 8 d to 21 d.


CONCLUSIONS
Local transfection of the CTLA(4)Ig-Adenovirus vector is a simple and effective way to prolong the survival of alloskin grafts.
Luo
G
G
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
Wu
J
J
Yi
S
S
D000069594:Abatacept; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics*; D060908:CTLA-4 Antigen; D015316:Genetic Therapy; D006085:Graft Survival; D018796:Immunoconjugates; D051379:Mice; D016038:Skin Transplantation; D014162:Transfection; D014184:Transplantation, Homologous
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556707:Ctla4 protein, mouse; D018796:Immunoconjugates; D000069594:Abatacept