[The significance and the role of TNFalpha and NO in the early renal damage in burned rats complicated with endotoxemia].
OBJECTIVE
To explore the role and the significance of TNFalpha and NO in the early renal damage in burned rats complicated with endotoxemia.


METHODS
The rats inflicted with 20% TBSA III degree burn and injected intraperitoneally with endotoxin (1 mg/kg) were employed as the model of MOD. The renal pathomorphology was observed at 0.5, 1, 3, 6, 12, 24, 48, 72 postburn hours. Concomitantly, the changes of renal and serum TNFalpha and NO were investigated and in situ hybridization for TNFalpha mRNA and immunohistochemical staining for iNOS were performed.


RESULTS
The expressions of renal iNOS and TNFalpha mRNA, the NO synthesis and the serum levels of NO and TNFalpha in BCEG (burns complicated with endotoxin group) were obviously higher and appeared earlier than those in SBG (simple burns group) and SEG(simple endotoxin group).


CONCLUSIONS
The renal TNFalpha and NO increased significantly in rats subjected to burn complicated with endotoxemia, which might be important factors causing renal damage and the alterations in renal hemodynamics.
Feng
J
J
Insititute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China.
Liu
Y
Y
Wang
X
X
D000818:Animals; D002056:Burns / Q000150:complications*; D019446:Endotoxemia / Q000473:pathology*; D005260:Female; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology*; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000032:analysis; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics / Q000502:physiology*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat