Inhibitory effect of tumor cell proliferation and induction of G2/M cell cycle arrest by panaxytriol.
Panaxytriol, a polyacetylenic compound, isolated from red ginseng (Panax ginseng C.A. Meyer), was studied to determine its effects on the growth and cell cycle of tumor cell lines. The compound showed both significant cytotoxicity and inhibition of DNA syntheses in various tumor cells tested. For P388D1, a mouse lymphoma cell line, IC50 values for cytotoxicity and inhibition of DNA synthesis were 3.1 and 0.7 microg/ml, respectively. The cytotoxic effect of panaxytriol was both time- and dose-dependent. It also induced the cell cycle arrest of P388D1 at the G2/M phase, which was measured through flow cytometry. Particularly, the proportion of cells in the G2/M phase of the cell cycle increased from 9 % to 26 and 48 %, respectively, after 24 and 36 h exposure to panaxytriol at 5 microg/ml. There were corresponding decreases in the proportion of cells at the G0/G1 phase. The S phase also decreased during the 36-h treatment.
Kim
Ji Yeon
JY
Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.
Lee
Ki-Won
KW
Kim
Sun-Ho
SH
Wee
Jae Joon
JJ
Kim
Young-Suk
YS
Lee
Hyong Joo
HJ
D016475:3T3 Cells; D000480:Alkynes; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D053281:Enediynes; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008518:Medicine, East Asian Traditional; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D015394:Molecular Structure; D017239:Paclitaxel / Q000494:pharmacology; D005894:Panax; D010936:Plant Extracts / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D020298:U937 Cells
D000480:Alkynes; D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D053281:Enediynes; D005233:Fatty Alcohols; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C057682:panaxytriol; C022616:thiazolyl blue; D017239:Paclitaxel