title: Inhibition of alpha(1-6)-linked fucose decreases inner retinal cells and increases photoreceptors in chicken retinal reaggregates.
abstract: We investigated the developmental role of alpha(1-6)-linked fucose, applying Aleuria aurantia lectin to a specific retinal regeneration system. Thereby, dissociated retinal cells of chicken embryos reaggregate, proliferate, and differentiate in vitro into histotypical spheres, so-called retinospheroids. Under the influence of A. aurantia lectin, processes of proliferation, differentiation and histogenesis of retinospheroids were disturbed. Extending these in vitro studies, we here show that A. aurantia lectin treatment decreases cells of the inner half retina and their processes into inner plexiform layer areas, as revealed by quantitative enzyme histochemistry for butyryl- and acetylcholinesterase, and immunohistochemistry using antibodies to acetylcholinesterase, Pax-6, calbindin-D, and F11. Concomitantly, the number of rod and red/green photoreceptors dramatically increases, using the antibodies rho4D2 and CERN901 (both specific for rods) and CERN906 (specific for red/green cones). These findings show that glycoproteins exhibiting fucose in alpha(1-6)-linkage are involved in processes determining retinal cell fate, strongly shifting the relative ratio of cells of the inner towards cells of the outer retina.
authors.affiliation: Technische Universität Darmstadt, Institut für Zoologie, Darmstadt, Germany. willbold@bio.tu-darmstadt.de
authors.forename: E
authors.initials: E
authors.lastname: Willbold
authors.forename: A
authors.initials: A
authors.lastname: Rothermel
authors.forename: J
authors.initials: J
authors.lastname: Huhn
authors.forename: S
authors.initials: S
authors.lastname: Stelck
authors.forename: P G
authors.initials: PG
authors.lastname: Layer
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D025042:Amacrine Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism; D064026:Calbindins; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D005136:Eye Proteins; D005643:Fucose / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D010786:Photoreceptor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012097:Repressor Proteins; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017299:Rod Opsins / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D064026:Calbindins; D005136:Eye Proteins; D006023:Glycoproteins; D018398:Homeodomain Proteins; D037102:Lectins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D017299:Rod Opsins; D064030:S100 Calcium Binding Protein G; C064549:lectin, Aleuria aurantia; D005643:Fucose; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase