title: Troponin-I interacts with the Met47 region of skeletal muscle actin. Implications for the mechanism of thin filament regulation by calcium.
abstract: Striated muscles are regulated by Ca(2+) via the thin filament proteins troponin (Tn) and tropomyosin (Tm). In the absence of Ca(2+), contraction is inhibited, whereas myosin-actin interaction and contraction can take place in its presence. Although it is well established that the interaction of troponin-I (TnI), the inhibitory subunit of Tn, with actin is required for the inhibition process and that there are two separate actin-binding regions in TnI that interact with actin, the molecular mechanism of this inhibition process is still not clear. Using TnI mutants with photocrosslinking probes attached to genetically engineered cysteine residues in each of the two actin-binding regions, we show that both regions are close to Met47 of actin in its outer domain. It has been proposed that the Ca(2+)-induced activation of contraction involves the movement of Tm from the outer to the inner domain of the actin filament. On the basis of our results presented here, we propose that the position of Tm at the outer domain of actin in the Ca(2+)-free state is stabilized by the presence of TnI over actin's outer domain via mutual interactions of all three components. In the presence of Ca(2+), TnI's actin-binding regions dissociate from actin allowing Tm to move toward actin's inner domain.
authors.affiliation: Muscle Research Group, Boston Biomedical Research Institute, Watertown, MA 02472, USA. yinluo@bbri.org
authors.forename: Yin
authors.initials: Y
authors.lastname: Luo
authors.forename: John
authors.initials: J
authors.lastname: Leszyk
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Li
authors.forename: John
authors.initials: J
authors.lastname: Gergely
authors.forename: Terence
authors.initials: T
authors.lastname: Tao
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D019811:Hydroxylamine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009154:Mutation; D010449:Peptide Mapping; D011485:Protein Binding; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011817:Rabbits; D019210:Troponin I / Q000235:genetics / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D000199:Actins; D019210:Troponin I; D019811:Hydroxylamine; D008715:Methionine; D014357:Trypsin; D002118:Calcium