A three-residue signal confers localization of a reporter protein in the inner nuclear membrane.
By alignment to the carboxy-terminal-deduced aa sequence of human cytomegalovirus glycoprotein B (gB), conserved hexameric aa motifs with putative function for localization in the inner nuclear membrane (INM) were identified in the nucleoplasmic tails of herpes simplex virus type 1 gB and of the cellular lamin B receptor. Fusion of the respective hexamers to CD8 as a reporter redirected transport of the chimeras into the INM, suggesting their functioning as modular signal motifs. Consecutive experiments showed that the three-residue motif RxR represents a consensus sequence which is sufficient for localization of the CD8 reporter in the INM.
Meyer
Grit
G
Institut für Virologie, Philipps-Universität, Robert-Koch-Strasse 17, DE-35037 Marburg, Germany.
Gicklhorn
Dorothee
D
Strive
Tanja
T
Radsak
Klaus
K
Eickmann
Markus
M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000032:analysis / Q000235:genetics; D019556:COS Cells; D016384:Consensus Sequence; D017930:Genes, Reporter; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000737:chemistry*; D019913:Nuclear Localization Signals; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000032:analysis; D016415:Sequence Alignment; D014162:Transfection; D014759:Viral Envelope Proteins / Q000737:chemistry; D000097682:Lamin B Receptor
D016827:CD8 Antigens; D019913:Nuclear Localization Signals; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D000097682:Lamin B Receptor; C040495:glycoprotein B, Simplexvirus