[Detection and proliferation of hemorrhagic fever virus in chigger mites].
OBJECTIVE
To study the impact of Leptotromhidium scutellaris as a vector in transmission of hemorrhagic fever.


METHODS
Reverse transcription-polymerase chain reaction (PCR) and in situ hybridization technique were used to detect RNA of hemorrhagic fever with renal syndrome virus (HFRSV). TCID(50)/ml of HFRSV were titrated for larva and nymph of chigger mites periodically.


RESULTS
RNA of HFRSV was detected in the tissues of chigger mites, such as ovary cells, etc. by PCR and in situ hybridization. Titration of TCID(50)/ml showed that HFRSV could be transmitted transstadially and proliferated in chigger mites.


CONCLUSIONS
It provides direct evidence at molecular level for the role of chigger mite as a vector in transmission of HFRSV with theoretical and practical importance in prevention of HFRS.
Zhang
Y
Y
Institute for Military Medicine, Nanjing Command of PLA, Nanjing 210002.
Zhu
J
J
Tang
J
J
Li
X
X
Wu
G
G
D000818:Animals; D018063:Hantaan virus / Q000254:growth & development / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000517:prevention & control / Q000635:transmission*; D007303:Insect Vectors / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D014324:Trombiculidae / Q000821:virology*
D012367:RNA, Viral