Fusogenic activity of reconstituted newcastle disease virus envelopes: a role for the hemagglutinin-neuraminidase protein in the fusion process.
Enveloped viruses, such as newcastle disease virus (NDV), make their entry into the host cell by membrane fusion. In the case of NDV, the fusion step requires both transmembrane hemagglutinin-neuraminidase (HN) and fusion (F) viral envelope glycoproteins. The HN protein should show fusion promotion activity. To date, the nature of HN-F interactions is a controversial issue. In this work, we aim to clarify the role of the HN glycoprotein in the membrane fusion step. Four types of reconstituted detergent-free NDV envelopes were used, on differing in their envelope protein contents. Fusion of the different virosomes and erythrocyte ghosts was monitored using the octadecyl rhodamine B chloride assay. Only the reconstituted envelopes having the F protein, even in the absence of HN protein, displayed residual fusion activity. Treatment of such virosomes with denaturing agents affecting the F protein abolished fusion, indicating that the fusion detected was viral protein-dependent. Interestingly, the rate of fusion in the reconstituted systems was similar to that of intact viruses in the presence of the inhibitor of HN sialidase activity 2,3-dehydro-2-deoxy-N-acetylneuraminic acid. The results show that the residual fusion activity detected in the reconstituted systems was exclusively due to F protein activity, with no contribution from the fusion promotion activity of HN protein.
Cobaleda
C
C
Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Plaza Doctores de la Reina s/n, Edificio Departamental, Lab109, 37007 Salamanca, Spain.
Muñoz-Barroso
I
I
Sagrera
A
A
Villar
E
E
D000818:Animals; D004910:Erythrocyte Membrane / Q000378:metabolism; D015707:HN Protein / Q000737:chemistry / Q000502:physiology*; D007700:Kinetics; D008081:Liposomes; D008561:Membrane Fusion; D009522:Newcastle disease virus / Q000502:physiology*; D013552:Swine; D014760:Viral Fusion Proteins / Q000502:physiology*
D015707:HN Protein; D008081:Liposomes; D014760:Viral Fusion Proteins