[The study of low dose radiation inducing hormesis effect on hematopoietic system].
OBJECTIVE
To study the hormesis effect on hematopoietic system induced by low dose radiation.


METHODS
CFU-GM and BFU-E were cultured in methylcellulose semi-solid culture system, levels of GM-CSF and IL-3 were assayed by ELISA and mRNA levels of GM-CSF, G-CSF, IL-3 by in situ hybridization, narrow line hybridization and Northern blot.


RESULTS
(1) The in vitro yields of CFU-GM and BFU-E from radiated mice was higher than that from the control. (2) The serum protein level of GM-CSF increased obviously than that of controls; (3) mRNA levels of GM-CSF and G-CSF were also increased.


CONCLUSIONS
There is hormesis effect on hematopoietic system induced by low dose radiation, which may be related to the increasing of cytokines.
Wang
G
G
Department of Hematology, The First Hospital of Norman Bethune Medical University, Changchun 130021, China.
Tan
Y
Y
Zhang
F
F
Li
W
W
Yang
L
L
Ma
L
L
D000818:Animals; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D055014:Granulocyte-Macrophage Progenitor Cells / Q000378:metabolism / Q000528:radiation effects*; D006706:Homeostasis / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014965:X-Rays
D016178:Granulocyte-Macrophage Colony-Stimulating Factor