title: Antiviral activity of seven iridoids, three saikosaponins and one phenylpropanoid glycoside extracted from Bupleurum rigidum and Scrophularia scorodonia.
abstract: As part of our screening of antiviral agents from medicinal plants, 11 compounds from plant origin (Bupleurum rigidum and Scrophularia scorodonia), three saikosaponins, seven iridoids and one phenylpropanoid glycoside were tested in vitro against herpes simplex type I (HSV-1), vesicular stomatitis virus (VSV) and poliovirus type 1. Five of these compounds showed antiviral activity against VSV. The percentages of cellular viability at the non-toxic limit concentrations of the active compounds were: verbascoside 53.6 % at 500 microg/ml, 8-acetylharpagide 32.1 % at 500 microg/ml, harpagoside 43.3 % at 450 microg/ml, scorodioside 47.8 % at 500 microg/ml and buddlejasaponin IV 56.9 % at 25 microg/ml. Although none of the saikosaponins were active against HSV-1, the iridoid scorodioside showed moderate in vitro anti-HSV-1 activity (30.6 % at 500 microg/ml). However, none of the compounds tested in this survey had any effect against poliovirus.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University Complutense, Madrid, Spain. naber@eucmax.sim.ucm.es
authors.forename: Paulina
authors.initials: P
authors.lastname: Bermejo
authors.forename: María José
authors.initials: MJ
authors.lastname: Abad
authors.forename: Ana María
authors.initials: AM
authors.lastname: Díaz
authors.forename: Lidia
authors.initials: L
authors.lastname: Fernández
authors.forename: Javier
authors.initials: J
authors.lastname: De Santos
authors.forename: Sandra
authors.initials: S
authors.lastname: Sanchez
authors.forename: Lucinda
authors.initials: L
authors.lastname: Villaescusa
authors.forename: Luis
authors.initials: L
authors.lastname: Carrasco
authors.forename: Alicia
authors.initials: A
authors.lastname: Irurzun
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D029972:Bupleurum; D002240:Carbohydrate Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D039823:Iridoids; D008519:Medicine, Traditional; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D017955:Poliovirus / Q000187:drug effects; D011714:Pyrans / Q000494:pharmacology*; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins; D028083:Scrophulariaceae; D014709:Vero Cells / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects*; D059808:Polyphenols
chemical_list: D000998:Antiviral Agents; D005960:Glucosides; D039823:Iridoids; D009828:Oleanolic Acid; D010636:Phenols; D010936:Plant Extracts; D011714:Pyrans; D012502:Sapogenins; D012503:Saponins; C058956:acteoside; D059808:Polyphenols; C025759:saikosaponin D