PrP(Sc) is not detected in peripheral blood leukocytes of scrapie-infected sheep: determining the limit of sensitivity by immunohistochemistry.
Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrP(Sc) by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrP(Sc)-positive cells were detected in 2.05% +/- 0.28% of 3 x 10(6) DRLN cells, but were not detected in 3 x 10(6) PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrP(Sc)-positive cells in 3 x 10(6) PBLs.
Herrmann
Lynn M
LM
USDA Agricultural Research Service ADRU, Pullman, Washington 99164, USA. lherrman@vetmed.wsu.edu
Baszler
Timothy V
TV
Knowles
Donald P
DP
Cheevers
William P
WP
D000818:Animals; D000911:Antibodies, Monoclonal; D007150:Immunohistochemistry / Q000592:standards*; D007962:Leukocytes / Q000737:chemistry*; D018620:PrPSc Proteins / Q000032:analysis* / Q000276:immunology; D012608:Scrapie / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012756:Sheep
D000911:Antibodies, Monoclonal; D018620:PrPSc Proteins