[The inhibition of prourokinase gene transfer on deposition of platelets on rabbit carotid artery intima].
OBJECTIVE
To observe the expression of pro-urokinase (proUK) gene in rabbit carotid artery transfected with replication-deficient adenovirus vector containing proUK gene (Ad/prouk) and the deposition of platelet on the same injured vessel.


METHODS
Ad/proUK (Ad/proUK, 3 x 10(10) pfu/ml) was injected into the right carotid artery locally. Wild type adenovirus (Ad) was locally injected into the left carotid artery as self-control. The expression of proUK gene was investigated by immunohistochemistry assay. After injury of the gene-transfected vessel by electric stimulation, the deposition of (111) In-labeled platelet was quantitatively observed. The thrombosis was observed with HE-stained vessel section.


RESULTS
There were lots of proUK granules in the endothelium of Ad/proUK gene-transfected vessel. The differences in (111) In-platelets deposition per gram dry weight vessel segment were significant between Ad/proUK and Ad transfected control vessels [(4.60 +/- 0.93) x 10(7)/g vs control (27.95 +/- 4.93) x 10(7)/g, P < 0.01)]. The HE-stained vessel section showed that there were only small thrombi in proUK gene-transfected vessel but massive thrombi almost blocked up the whole vessel in control vessel.


CONCLUSIONS
The proUK gene-transfected vessel can obviously inhibit (111) In-labeled platelet deposition on injured vessel and thus thrombosis.
Zhu
F
F
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Chen
G
G
Fu
A
A
Tang
C
C
Zhou
A
A
Tang
J
J
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002339:Carotid Arteries / Q000473:pathology; D022761:Gene Transfer, Horizontal; D015316:Genetic Therapy; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics*; D013927:Thrombosis / Q000517:prevention & control*; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics* / Q000502:physiology
D011994:Recombinant Proteins; D014568:Urokinase-Type Plasminogen Activator; C067551:saruplase