[Study on the abnormal gene expression of inducible nitric oxide synthase (iNOS) in rabbit myocardium after high voltage electrical injury].
OBJECTIVE
To elucidate the effects of high voltage electrical injury on the mRNA expression of iNOS And endothelin-1 (ET-1) in rabbit myocardium.


METHODS
Rabbits inflicted by high voltage electrical injury were employed as the model. Reverse transcription PCR (RT-PCR) and serum enzyme measurement were used in the detection of the expression levels of iNOS mRNA and ET-1 mRNA of rabbit myocardial tissue and plasma creatine kinase (CK) content.


RESULTS
The expression of iNOS mRNA in rabbit myocardial tissue after high voltage electrical injury increased at 6 to 8 postburn hours (PBH) and decreased thereafter. The plasma contents of CK and ET-1 mRNA reached their peak values at 2 PBH.


CONCLUSIONS
The increase of iNOS mRNA might protect myocardial tissue from electrical injury.
Feng
Z
Z
Department of Burn Surgery, He Nan Provincial Electrical Power Hospital, Zhengzhou 450052, He Nan P. R. China.
Li
J
J
Jiang
G
G
D000818:Animals; D002058:Burns, Electric / Q000201:enzymology*; D019332:Endothelin-1 / Q000235:genetics; D009206:Myocardium / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits
D019332:Endothelin-1; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II