Oral flavonoids, chromocarb diethylamine salt and cyaninosides chloride, to eliminate lipoperoxidation postvitrectomy.
This study was undertaken to determine the concentration of malondialdehyde, an end product of lipoperoxidation, in lens and retinal tissue postvitrectomy associated with oral administration of antioxidant flavonoids cyaninosides chloride and chromocarb diethylamine salt or N -acetylcysteine. Fifty adult pigmented rabbits were divided into five groups: (1) controls (normal eyes, malondialdehyde concentration in lens and retina); (2) vitrectomy with BSS Plus (malondialdehyde level measured 2hr after vitrectomy); (3) vitrectomy with BSS Plus and pretreatment with oral cyaninosides chloride 100mg kg day(-1)for 3 weeks (malondialdehyde level measured 2hr after surgery); (4) vitrectomy with BSS Plus and pretreatment with oral chromocarb diethylamine salt 100 mg kg day(-1)for 3 weeks (malondialdehyde level measured 2hr after surgery); and (5) vitrectomy with BSS Plus and pretreatment with oral N -acetylcysteine 200 mg kg day(-1)for 3 weeks (malondialdehyde level measured 2hr after surgery). Lens and retina samples were used to determine malondialdehyde levels using ion-pairing high performance liquid chromatography. Statistical analysis was done using analysis of variance (P<0.05). The content of malondialdehyde in the normal lens was 0.036 +/- 0.017 microg g(-1); in the vitrectomized groups the malondialdehyde concentrations were as follows: (2) 0.027 +/- 0.013 microg g(-1); (3) under detection limit (detection limit=1.75x e-3 microg g(-1)); (4) under detection limit; and (5) 0.020 +/- 0.006 microg g(-1). The results showed that the malondialdehyde concentration in the normal retina was 1.160 +/- 0.361 microg g(-1), while in the vitrectomized groups with or without pretreatment (cyaninosides chloride, chromocarb diethylamine salt, and N -acetylcysteine) the malondialdehyde levels were 2.091 +/- 0.982 microg g(-1), 0.069 +/- 0.024 microg g(-1), 0.082 +/- 0.027 microg g(-1), and 0.215 +/- 0.134 microg/g(-1), respectively, all significantly different from the normal eyes (P<0.05). Vitrectomy induced increased malondialdehyde levels in the retina. Oral flavonoids are an effective protective therapy for surgically induced lipoperoxidation, especially in the retina.
Manzanas
Lucia
L
Instituto Universitario de Oftalmobiología Aplicada (IOBA), Facultad de Medicina, Universidad de Valladolid, c/Ramón y Cajal, 7, Valladolid 47010, Spain. luciamanzanas@hotmail.com
Jesús del Nozal
Maria
M
Marcos
Manuel Angel
MA
Cordero
Yolanda
Y
Bernal
José Luis
JL
Goldschmidt
Pablo
P
Pastor
José Carlos
JC
D000284:Administration, Oral; D000818:Animals; D000872:Anthocyanins; D000975:Antioxidants / Q000494:pharmacology*; D002867:Chromones / Q000494:pharmacology; D004048:Diethylamines / Q000494:pharmacology; D004338:Drug Combinations; D005419:Flavonoids / Q000494:pharmacology; D007908:Lens, Crystalline / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis; D011184:Postoperative Period; D011817:Rabbits; D012160:Retina / Q000737:chemistry; D014821:Vitrectomy / Q000009:adverse effects*
D000872:Anthocyanins; D000975:Antioxidants; D002867:Chromones; D004048:Diethylamines; D004338:Drug Combinations; D005419:Flavonoids; D008315:Malondialdehyde; C031334:chromocarb diethylamine; C020386:keracyanin