title: Large-scale plant proteomics.
abstract: Large-scale and high throughput approaches increasingly play an essential role in the study of biological systems, which are per se highly complex. Therefore, they need to be examined by these extensive methods to receive information about the large genomic and proteomic networks. In plant biology, this purpose has a strong support through the accessability of the complete genome sequence of the model plant Arabidopsis thaliana. This brief review intends to focus on the basics and the state-of-the-art of these high-throughput technologies and their application to plant proteomics. It describes protein microarrays, the use of antibodies, 2-DE and MS methods and the yeast two hybrid system, which are emerging as the major technologies for plant proteomics.
authors.affiliation: Max Planck Institute of Molecular Genetics, Berlin, Germany. kersten@molgen.mpg.de
authors.forename: Birgit
authors.initials: B
authors.lastname: Kersten
authors.forename: Lukas
authors.initials: L
authors.lastname: Bürkle
authors.forename: Eckehard J
authors.initials: EJ
authors.lastname: Kuhn
authors.forename: Patrick
authors.initials: P
authors.lastname: Giavalisco
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Konthur
authors.forename: Angelika
authors.initials: A
authors.lastname: Lueking
authors.forename: Gerald
authors.initials: G
authors.lastname: Walter
authors.forename: Holger
authors.initials: H
authors.lastname: Eickhoff
authors.forename: Ulrich
authors.initials: U
authors.lastname: Schneider
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D023281:Genomics; D013058:Mass Spectrometry / Q000379:methods; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D020798:Two-Hybrid System Techniques
chemical_list: D020543:Proteome