Structural studies on kappa-carrageenan derived oligosaccharides.
Oligosaccharides were prepared through mild hydrochloric acid hydrolysis of kappa-carrageenan from Kappaphycus striatum carrageenan. Three oligosaccharides were purified by strong-anion exchange high-performance chromatography. Their structure was elucidated using mass spectral and NMR data. Negative-ion electrospray ionization (ESI) mass spectra at different fragmentor voltages provided the molecular weight of the compounds and unraveled the fragmentation pattern of the kappa-carrageenan oligosaccharides. 2D NMR techniques, including 1H-(1)H COSY, 1H-(1)H TOCSY and 13C-(1)H HMQC, were performed to determine the structure of a trisulfated pentasaccharide. 1D NMR and ESIMS were used to determine the structures of a kappa-carrageenan-derived pentasaccharide, heptasaccharide, and an undecasaccharide. All the oligosaccharides characterized have a 4-O-sulfo-D-galactopyranose residue at both the reducing and nonreducing ends.
Yu
Guangli
G
Marine Drug and Food Institute, Ocean University of Qingdao, 266003, Qingdao, People's Republic of China.
Guan
Huashi
H
Ioanoviciu
Alexandra S
AS
Sikkander
Sulthan A
SA
Thanawiroon
Charuwan
C
Tobacman
Joanne K
JK
Toida
Toshihiko
T
Linhardt
Robert J
RJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002351:Carrageenan / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D000461:Rhodophyta / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D009844:Oligosaccharides; D002351:Carrageenan