Chemical incorporation of 1-methyladenosine into oligonucleotides.
The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.
Mikhailov
Sergey N
SN
Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Rozenski
Jef
J
Efimtseva
Ekaterina V
EV
Busson
Roger
R
Van Aerschot
Arthur
A
Herdewijn
Piet
P
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006358:Hot Temperature; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C002230:1-methyladenosine; D000241:Adenosine