[Study on expression of P53mt protein in skin of patient with arseniasis caused by coal-burning].
OBJECTIVE
To study expression of P53mt protein in skin of patient with arseniasis caused by coal-burning.


METHODS
According to pathological diagnosis, the patients were divided into carcinoma group (A group, 18 cases), pre-carcinoma group (B group 11 cases) and general pathological change group (C group, 39 cases). Immuno-histochemical technique of two steps was used to detect expression of P53 mt protein.


RESULTS
Among A, B and C groups, the positive cell densities of P53mt protein were 38.07 +/- 29.00, 17.16 +/- 15.00 and 4.05 +/- 8.24 respectively, but no positive cell in normal group; there was statistical significance among A, B and C groups (A and C: P < 0.01, A and B: P < 0.01, B and C: P < 0.05); positive percentages of A, B and C groups were 88.89%, 72.73% and 25.64% (A and C: P < 0.01; B and C: P < 0.05), respectively, for difference of grade constitution, there was statistical significance (P < 0.01) among three groups; grade II and III made up 38.89% in A group, but 9.09% in B group and 2.56% in C group.


CONCLUSIONS
P53mt gene play a certain role in the development of skin cancer from arseniasis caused by coal-burning; the detection of P53mt protein can show whether pathological changes of skin due to the disease is malignant or benign.
Hu
C
C
Department of Public Health, Guiyang Medical College, Guiyang, 550004, China.
Zhang
A
A
Huang
X
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D020261:Arsenic Poisoning / Q000150:complications / Q000378:metabolism*; D003031:Coal / Q000009:adverse effects*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D012867:Skin / Q000737:chemistry*; D012878:Skin Neoplasms / Q000209:etiology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D003031:Coal; D016159:Tumor Suppressor Protein p53