[The hEGF expression of gene--transferred human epithelium after its grafting on porcine surgical wound].
OBJECTIVE
To investigate the hEGF expression of gene -- transferred human epithelium after its grafting on porcine surgical wound.


METHODS
Gene -- transferred and normal epithelia were implanted on the biological silicone membrane and then grafted on porcine surgical wound. The tissue samples were harvested from the wound and detected for the hEGF expression with immunohistochemical method on 1, 3, 5, 8 and 12 post -- grafting days.


RESULTS
There existed positive hEGF expression in the wound grafted with gene -- transferred epithelia and negative hEGF expression in the wound grafted with normal epithelium.


CONCLUSIONS
Gene -- transferred epithelium could express hEGF on the grafted wound.
Dong
Z
Z
Department of Burns and Plastic Surgery, Corps Hospital, Chinese People's Armed Police Force of Shanghai, Shanghai 201103 P.R. China.
Chen
Y
Y
Dai
F
F
D000818:Animals; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D004848:Epithelium / Q000378:metabolism / Q000637:transplantation*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016038:Skin Transplantation / Q000379:methods*; D013552:Swine; D014162:Transfection; D014945:Wound Healing / Q000235:genetics / Q000502:physiology
D004815:Epidermal Growth Factor