title: Furanoligularenone, an eremophilane from Ligularia fischeri, inhibits the LPS-induced production of nitric oxide and prostaglandin E2 in macrophage RAW264.7 cells.
abstract: Furanoligularenone (1), a known eremophilane, was identified from Ligularia fischeri (Compositae) together with 3-oxo-8alpha-hydroxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide (2) and 3-oxo-8alpha-methoxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide (3), by its potent inhibition of LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW 264.7 cells. Compound 1 also suppressed the expression of iNOS and COX-2 mRNA and protein in a dose-dependent manner. Taken together, compound 1 inhibits the production of NO and PGE2 at the transcription of iNOS and COX-2 genes, and would be responsible for the anti-inflammatory activities of the Ligularia fischeri.
authors.affiliation: Anticancer Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Jeong-Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Koo
authors.forename: Hang Sub
authors.initials: HS
authors.lastname: Kim
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Hong
authors.forename: Jai Seup
authors.initials: JS
authors.lastname: Ro
authors.forename: Kyong Soon
authors.initials: KS
authors.lastname: Lee
authors.forename: Jung Joon
authors.initials: JJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D019659:Asteraceae; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051546:Cyclooxygenase 2; D015237:Dinoprost / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D016328:NF-kappa B / Q000378:metabolism; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D010936:Plant Extracts / Q000494:pharmacology; D000081223:Polycyclic Sesquiterpenes; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C455060:3-oxo-8alpha-hydroxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide; C455059:3-oxo-8alpha-methoxy-10alphaH-eremophila-1,7(11)-dien-12,8beta-olide; D007527:Isoenzymes; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009281:Naphthalenes; D010936:Plant Extracts; D000081223:Polycyclic Sesquiterpenes; D012333:RNA, Messenger; D012717:Sesquiterpenes; C018286:eremophilane compounds; C455061:furanoligularenone; D009569:Nitric Oxide; D015237:Dinoprost; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases