Reassessment of CD62L as a marker of pre-effector T cells in the tumor draining lymph nodes of head and neck cancer patients.
OBJECTIVE
CD62L was evaluated as a determinant of human pre-effector T cells.


METHODS
Phenotype and cytokine secretion profiles of CD62L cells were determined based on activation status.


RESULTS
CD62L(Low) T cells demonstrated significantly higher secretion of interleukin (IL)-10 and interferon (IFN)-gamma than did CD62L(High) T cells. After activation, the majority of cells expressed high levels of the CD62L surface marker. Postactivation levels of IL-10 production remained elevated or unchanged. In a murine B16 melanoma model, freshly isolated CD62L(Low) tumor draining lymph nodes (TDLN) T cells showed increased secretion of IL-2 and IL-4 but not of IL-10 or IFN-gamma. The surface expression of CD62L and cytokine secretion patterns were maintained after activation with concomitant increases in IL-10.


CONCLUSIONS
Our results provide evidence that CD62L(Low) T cells in TDLNs of progressively growing squamous cell carcinoma of the head and neck differ phenotypically and functionally from those of mouse origin.


CONCLUSIONS
Characterization of this human CD62L(Low) T cell population provides initial insight regarding novel surface markers in TDLN T cells that might correlate with antitumor reactivity.
Strome
Scott E
SE
Department of Otorhinolaryngology, Mayo Graduate and Medical Schools, Mayo Clinic, Rochester, Minnesota 55905, USA. strome.scott@mayo.edu
Martin
Elizabeth
E
Flies
Dallas
D
Khurana
Dianne
D
Salomao
Diva
D
Tamada
Koji
K
Chapoval
Andrei I
AI
Kasperbauer
Jan L
JL
Olsen
Kerry D
KD
Chen
Lieping
L
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006258:Head and Neck Neoplasms / Q000276:immunology*; D007378:Interleukins / Q000276:immunology; D019041:L-Selectin / Q000276:immunology*; D008213:Lymphocyte Activation; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014407:Tumor Cells, Cultured
D007378:Interleukins; D019041:L-Selectin