title: A simple and rapid assay of collagen-like polymer in crude lysate from Escherichia coli.
abstract: An assay for the quantification of collagen-like polymer (CLP) in Escherichia coli cells utilizing the specific reaction between collagenase and CLP is presented. It involves thermal treatment to precipitate non-CLP proteins, digestion of CLP by collagenase and detection of the absorbance of the liberated amino acids and peptides from CLP by a ninhydrin-based method. CLP concentration is determined from the absorbance measurement.
authors.affiliation: Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Building 16, Room 429, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Yin
authors.forename: Luke
authors.initials: L
authors.lastname: Tomycz
authors.forename: Gary
authors.initials: G
authors.lastname: Bonner
authors.forename: Daniel I C
authors.initials: DI
authors.lastname: Wang
mesh_terms: D003094:Collagen / Q000032:analysis*; D003124:Colorimetry; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology; D003012:Microbial Collagenase / Q000378:metabolism; D009555:Ninhydrin; D011108:Polymers / Q000032:analysis*
chemical_list: D011108:Polymers; D003094:Collagen; D003012:Microbial Collagenase; D009555:Ninhydrin