title: Reliable ELISAs showing differences between resistant and susceptible lines in hens orally inoculated with Salmonella Enteritidis.
abstract: Reliable ELISAs were investigated with the aim to select hen lines resistant to Salmonella Enteritidis and producing high levels of antibodies. In the first experiment, the relation between the humoral response and the bacteriological results was assessed on hens from the Y11 resistant line and the L2 susceptible line, orally inoculated with 10(8) CFU S. Enteritidis per animal. Anti-lipopolysaccharide (LPS) IgG titres were higher but the liver and spleen were less contaminated in hens from the Y11 line than in hens from the L2 line (p = 0.013, 0.031 and 0.026 respectively). In the second experiment, the hens were inoculated orally with 1.7 x 10(8) CFU S. Enteritidis per animal in order to select the ELISA methods showing the more significant differences. ELISAs were based on LPS, flagella, LPS from rough (LPS-R) and smooth strains (LPS-S) and detected IgG and IgM antibodies from sera and yolks. No between-line host response variation was observed in the yolk, with LPS-S and R antigens nor with anti-LPS IgM in the sera. Otherwise, significant differences were encountered between hen lines with the ELISAs performed on the sera detecting anti-LPS IgG, anti-flagella IgG or IgM (p = 0.017, 0.017 and p < 0.001 respectively). When comparing the kinetics of the selected ELISAs, the IgG antibodies against LPS detected between-line variations as early as 1 to 4 weeks pi, whereas with IgG against flagella, the differences were only detected at 1 and 2 weeks pi and with IgM against flagella, the differences were significant at 1, 2, 4 and 8 weeks pi. In conclusion, resistant hen lines producing higher levels of antibodies than the susceptible hen lines may be selected with these ELISAs.
authors.affiliation: AFSSA Ploufragan, BP, France. k.proux@ploufragan.afssa.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Proux
authors.forename: Eric
authors.initials: E
authors.lastname: Jouy
authors.forename: Catherine
authors.initials: C
authors.lastname: Houdayer
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Protais
authors.forename: Mike
authors.initials: M
authors.lastname: Dibb-Füller
authors.forename: Evelyne
authors.initials: E
authors.lastname: Boscher
authors.forename: Angélique
authors.initials: A
authors.lastname: Gillard
authors.forename: Patrice
authors.initials: P
authors.lastname: Gracieux
authors.forename: Florence
authors.initials: F
authors.lastname: Gilbert
authors.forename: Catherine
authors.initials: C
authors.lastname: Beaumont
authors.forename: Marion
authors.initials: M
authors.lastname: Duchet-Suchaux
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D002645:Chickens; D015169:Colony Count, Microbial; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D005407:Flagella / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology*; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D012680:Sensitivity and Specificity; D013047:Specific Pathogen-Free Organisms
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides