Structure of the beta-chain (B29) gene of the chicken B-cell receptor and conserved collinearity with genes for potential skeletal muscle sodium channel and growth hormone.
Abstract. Reported here is the cloning of the gene for the beta-chain (Ig beta) of the chicken B-cell receptor for antigen. A cDNA library made from B lymphocyte-derived DT40 cells was first screened with a beta-chain cDNA clone from rat. A full-length clone (1556 bp) was obtained encoding a 226-amino-acid beta-chain having 40% sequence identity with the highly conserved beta-chains present in mammals and producing mRNA of 1.7 kb. Clones containing the full B29 gene encoding chicken Ig beta were isolated and fully sequenced. As in mammals, the chicken B29 gene consists of six exons. The chicken B29 gene is somewhat larger (4336 bp) than that of rat (3.1 kb) and human (3.6 kb). Genome collinearity was found in the region of the chicken B29 gene. Genes for potential skeletal muscle sodium channel (2.2 kb upstream) and for growth hormone (2.3 kb downstream) were found in the same order and transcriptional orientation as in mammals, though the genes are more closely positioned in the chicken.
Katsukura
H
H
Life Science Course, Department of Chemistry, College of Science, Rikkyo University, Toshima-ku, Tokyo 171-8501, Japan.
Murakami
R
R
Chijiiwa
Y
Y
Otsuka
A
A
Tanaka
M
M
Nakashima
K
K
Ono
M
M
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D051925:CD79 Antigens; D002645:Chickens / Q000235:genetics* / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics; D008040:Genetic Linkage; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats; D011947:Receptors, Antigen, B-Cell / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015222:Sodium Channels / Q000235:genetics*; D013045:Species Specificity
D015703:Antigens, CD; D051925:CD79 Antigens; C497883:CD79B protein, human; C497884:Cd79b protein, mouse; C497885:Cd79b protein, rat; D018076:DNA, Complementary; D011947:Receptors, Antigen, B-Cell; D015222:Sodium Channels; D013006:Growth Hormone