"De novo" sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry.
Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
Shevchenko
Andrej
A
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. shevchenko@mpi-cbg.de
Chernushevic
Igor
I
Shevchenko
Anna
A
Wilm
Matthias
M
Mann
Matthias
M
D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010103:Oxygen Isotopes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D010103:Oxygen Isotopes; D010446:Peptide Fragments; D014357:Trypsin