Evaluation of select variables in the ion chromatographic determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples.
A full experimental design at two levels is applied for the estimation of the significance of select factors that may influence the ion chromatography (IC) determination of F-, Cl-, Br-, NO(-)3, SO(-2)4, and PO(-3)4 in serum samples. The factors studied are various sample deproteinization procedures, eluent composition, and flow rates. Deproteinization using either acetonitrile-NaOH or ultrafiltration can be used in order to obtain a significant protein removal before IC analysis; however, the former is recommended because it is less time-consuming and cheaper. Better resolution is obtained when a sodium hydroxide solution is used as the eluent. There is no influence of the sample's deproteinization procedures on the chromatographic resolution.
Benzo
Z
Z
Centro de Química, Instituto Venezolano de Investigaciones Científicas, IVIC, Caracas, Venezuela. zbenzo@ivic.ve
Escalona
A
A
Salas
J
J
Gómez
C
C
Quintal
M
M
Marcano
E
E
Ruiz
F
F
Garaboto
A
A
Bartoli
F
F
D000838:Anions / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans
D000838:Anions