Regulation of jejunal glucose transporter expression by forskolin.
We have investigated the effects of forskolin on enterocyte membrane expression of the glucose transporters, SGLT1 and GLUT2, which are thought to be the main entry and efflux pathways for glucose, respectively. Forskolin treatment increased SGLT1 but decreased GLUT2 expression in mid and lower villus enterocytes. No change in transporter expression was noted in upper villus cells. Likewise, cyclic AMP levels were raised in mid and lower but not upper villus cells. The implications of these data for glucose transport are discussed.
Williams
Mark
M
School of Biological Sciences, University of East Anglia, Norwich, UK.
Sharp
Paul
P
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D020895:Enterocytes / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D051273:Glucose Transporter Type 2; D015151:Immunoblotting; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D051269:Sodium-Glucose Transporter 1
D051273:Glucose Transporter Type 2; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D005576:Colforsin; D000242:Cyclic AMP