title: Neospora caninum and Waddlia chondrophila strain 2032/99 in a septic stillborn calf.
abstract: Characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. The organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with Waddlia chondrophila. An ELISA specific for Chlamydia spp., immunofluorescence tests using antibodies directed against Chlamydia spp. or Simkania negevensis, and PCR using Chlamydia-specific primers showed that the agent was distinct from Chlamydiae or S. negevensis. Determination of 16S and partial 23S ribosomal RNA gene sequences in combination with the PCR results and the morphological, antigenic and developmental characteristics provided evidence that the isolate 2032/99 can be classified as W. chondrophila or a closely related organism.
authors.affiliation: Federal Research Centre of Virus Diseases of Animals, Seestrasse 55, D-16868, Wusterhausen, Germany. klaus.henning@wus.bfav.de
authors.forename: K
authors.initials: K
authors.lastname: Henning
authors.forename: G
authors.initials: G
authors.lastname: Schares
authors.forename: H
authors.initials: H
authors.lastname: Granzow
authors.forename: U
authors.initials: U
authors.lastname: Polster
authors.forename: M
authors.initials: M
authors.lastname: Hartmann
authors.forename: H
authors.initials: H
authors.lastname: Hotzel
authors.forename: K
authors.initials: K
authors.lastname: Sachse
authors.forename: M
authors.initials: M
authors.lastname: Peters
authors.forename: M
authors.initials: M
authors.lastname: Rauser
mesh_terms: D000034:Abortion, Veterinary / Q000209:etiology* / Q000382:microbiology / Q000469:parasitology; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002695:Chlamydiales / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003048:Coccidiosis / Q000662:veterinary*; D004351:Drug Resistance; D005260:Female; D005333:Fetus / Q000382:microbiology / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000662:veterinary*; D008854:Microscopy, Electron / Q000662:veterinary; D008969:Molecular Sequence Data; D018688:Neospora / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary; D015597:Pregnancy Complications, Parasitic / Q000662:veterinary