[Autoantibodies to human thyroid peroxidase in immunoassay and immunoaffinity chromatography].
A biochemical system of radioimmunoassay of human thyroid peroxidase (TPO) with the use of specific autoantibodies was developed for the first time. This system includes lyophilized preparations of human autoantibodies to TPO, radioiodinated TPO, standards prepared from pure TPO, and the solid-phase protein A as a precipitating agent. An analytical system was used to study the TPO distribution in fractions of thyroid tissue homogenate. Differences in TPO concentrations in mitochondria, microsomes, and supernatant fluid depending on thyroid pathology and tissue storage conditions were found. The behavior of immobilized anti-TPO-autoantibodies in immunoaffinity chromatography of this microsomal antigen was studied.
Vashkevich
I I
II
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141 Belarus.
Kiseleva
E P
EP
Sviridov
O V
OV
Survilo
L I
LI
Strel'chenok
O A
OA
D001323:Autoantibodies / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods; D006801:Humans; D007453:Iodide Peroxidase / Q000032:analysis* / Q000276:immunology; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*; D013347:Subcellular Fractions / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology
D001323:Autoantibodies; D007457:Iodine Radioisotopes; D007453:Iodide Peroxidase