[The development of immune-mediated aplastic anemia in mice can be blocked by cimetidine].
OBJECTIVE
To investigate the effect of H(2) receptor antagonist cimetidine on the development of immune-mediated aplastic anemia (AA) in mice.


METHODS
The immune-mediated aplastic anemia mice model was induced by irradiation and half-matched allogeneic lymphocytes transfusion (ALT). The mice were divided into 3 groups: the irradiation alone control group, the irradiation + ALT group (AA group) and the irradiation + ALT + cimetidine group. In cimetidine group, each mouse was intraperitoneally injected cimetidine at different doses once a day for 8 days. On day 14, the bone marrow histology was examined.


RESULTS
In 0.5 mg, 1.0 mg and 2.0 mg x (10g)(-1) x d(-1) cimetidine groups, the percentages of hematopoietic tissue volume in bone marrow were (46.58 +/- 20.41)%, (51.88 +/- 24.94)% and (59.12 +/- 32.48)%, respectively, and all much higher than that in AA group [(19.99 +/- 10.98)%, P < 0.01], but were not different from that in irradiated control group [(53.81 +/- 31.72)%, P > 0.05].


CONCLUSIONS
The development of immune-mediated aplastic anemia in irradiated mice by half-matched allogeneic lymphocytes transfusion was blocked by H(2) receptor antagonist cimetidine.
Wang
M
M
Shenzhen Institute of Hematology, Department of Hematology of Shenzhen Red Cross Hospital, Shenzhen 518035, China.
Liao
J
J
Zhao
X
X
Lin
C
C
Jiang
Z
Z
Ma
X
X
D000741:Anemia, Aplastic / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D002927:Cimetidine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D006635:Histamine H2 Antagonists; D002927:Cimetidine