title: Small dose of exogenous surfactant combined with partial liquid ventilation in experimental acute lung injury: effects on gas exchange, haemodynamics, lung mechanics, and lung pathology.
abstract: A combination of exogenous surfactant and partial liquid ventilation (PLV) with perfluorocarbons should enhance gas exchange, improve respiratory mechanics and reduce tissue damage of the lung in acute lung injury (ALI). We used a small dose of exogenous surfactant with and without PLV in an experimental model of ALI and studied the effects on gas exchange, haemodynamics, lung mechanics, and lung pathology. ALI was induced by repeated lavages (PaO2/FIO2 less than 13 kPa) in 24 anaesthesized, tracheotomized and mechanically ventilated (FIO2 1.0) juvenile pigs. They were treated randomly with either a single intratracheal dose of surfactant (50 mg kg(-1), Curosurf, Serono AG, München, Germany) (SURF-group, n=8), a single intratracheal dose of surfactant (50 mg kg(-1), Curosurf) followed by PLV with 30 ml kg(-1) of perfluorocarbon (PF 5080, 3M, Germany) (SURF-PLV-group, n=8) or no further intervention (controls, n=8). Pulmonary gas exchange, respiratory mechanics, and haemodynamics were measured hourly for a 6 h period. In the SURF-group, the intrapulmonary right-to-left shunt (QS/QT) decreased significantly from mean 51 (SEM 5)% after lavage to 12 (2)%, and PaO2 increased significantly from 8.1 (0.7) to 61.2 (4.7) kPa compared with controls and compared with the SURF-PLV-group (P<0.05). In the SURF-PLV-group, QS/QT decreased significantly from 54 (3)% after induction of ALI to 26 (3)% and PaO2 increased significantly from 7.2 (0.5) to 30.8 (5.0) kPa compared with controls (P<0.05). Static compliance of the respiratory system (C(RS)), significantly improved in the SURF-PLV-group compared with controls (P<0.05). Upon histological examination, the SURF-group revealed the lowest total injury score compared with controls and the SURF-PLV-group (P<0.05). We conclude that in this experimental model of ALI, treatment with a small dose of exogenous surfactant improves pulmonary gas exchange and reduces the lung injury more effectively than the combined treatment of a small dose of exogenous surfactant and PLV.
authors.affiliation: Klinik für Anaesthesiologie und Operative Intensivmedizin, Charité, Medizinische Fakultaet der Humboldt-Universitaet, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.forename: H
authors.initials: H
authors.lastname: Lohbrunner
authors.forename: T
authors.initials: T
authors.lastname: Busch
authors.forename: A
authors.initials: A
authors.lastname: Sterner-Kock
authors.forename: M
authors.initials: M
authors.lastname: Deja
authors.forename: A
authors.initials: A
authors.lastname: Sarrafzadeh
authors.forename: U
authors.initials: U
authors.lastname: Neumann
authors.forename: U
authors.initials: U
authors.lastname: Kaisers
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D003131:Combined Modality Therapy; D006439:Hemodynamics; D021061:Liquid Ventilation; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D011659:Pulmonary Gas Exchange; D011663:Pulmonary Surfactants / Q000008:administration & dosage*; D012128:Respiratory Distress Syndrome / Q000473:pathology / Q000628:therapy*; D015656:Respiratory Mechanics; D013552:Swine
chemical_list: D011663:Pulmonary Surfactants; D002245:Carbon Dioxide; D010100:Oxygen