title: Expression and self-assembly of norwalk virus capsid protein from venezuelan equine encephalitis virus replicons.
abstract: The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.
authors.affiliation: Department of Epidemiology, Program in Infectious Diseases, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400, USA. rbaric@sph.unc.edu
authors.forename: Ralph S
authors.initials: RS
authors.lastname: Baric
authors.forename: Boyd
authors.initials: B
authors.lastname: Yount
authors.forename: Lisa
authors.initials: L
authors.lastname: Lindesmith
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Harrington
authors.forename: Shermalyn R
authors.initials: SR
authors.lastname: Greene
authors.forename: Fan-Chen
authors.initials: FC
authors.lastname: Tseng
authors.forename: Nancy
authors.initials: N
authors.lastname: Davis
authors.forename: Robert E
authors.initials: RE
authors.lastname: Johnston
authors.forename: David G
authors.initials: DG
authors.lastname: Klapper
authors.forename: Christine L
authors.initials: CL
authors.lastname: Moe
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D009663:Norwalk virus / Q000378:metabolism*; D012093:Replicon; D019065:Virus Assembly