[Inhibitory effects of recombinant human neurotrophin-4/5 protein on neurotoxicity caused by arsenic trioxide].
OBJECTIVE
To study preliminarily the inhibitory effects of recombinant human neurotrophin (hNT)-4/5 protein on neurotoxicity caused by arsenic trioxide (As(2)O(3)).


METHODS
Nerve cells of the chicken embryonic forebrain and PC12 cells were co-cultured with recombinant hNT-4/5 protein and As(2)O(3) (0 - 16 micromol/L) for 24 and 48 hours, respectively, to observe the survival of nerve cells and the outgrowth of PC12 cells, with clone expression and recombination techniques.


RESULTS
There was significant difference in survival rate of nerve cells between the experimental and control groups (P < 0.01) after co-culture for 48 hours, and survival rate and number of neuron outgrowth increased with the concentration of hNT-4/5 protein.


CONCLUSIONS
Recombinant hNT-4/5 protein could inhibit neurotoxicity caused by As(2)O(3), which provided a basis to find an anti-toxic factor in the environment by genetic engineering.
Lou
Y
Y
Department of Environmental Health, Xinjiang Medical College, Urumqi 830054.
Wang
G
G
Huang
Y
Y
Yin
M
M
Dai
J
J
Ying
K
K
Gu
S
S
Liu
J
J
Xie
Y
Y
D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D002470:Cell Survival; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D004781:Environmental Exposure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010087:Oxides / Q000633:toxicity*; D016716:PC12 Cells; D016548:Prosencephalon / Q000166:cytology; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D001152:Arsenicals; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D010087:Oxides; D011994:Recombinant Proteins; C071951:neurotrophin 5; C073922:neurotrophin 4; D000077237:Arsenic Trioxide