title: Fas ligand mRNA expression in the mouse central nervous system.
abstract: Fas ligand (FasL) expressing cells delete Fas bearing T cells, thereby enabling privileged immune status in the brain. Although the presence of FasL immunoreactivity has been shown in various cell types in the central nervous system, the precise in vivo distribution of FasL mRNA in mammals is not known. Accordingly, we localized intense FasL mRNA signals in neuroglial cells mainly within the white matter regions. Using a combined labeling technique of immunocytochemistry and in situ hybridization, we confirmed that FasL signals were due to neuroglial cells rather than neurons. This study shows that FasL mRNA is constitutively expressed in the normal mouse brain, and suggests that the Fas/FasL system protects the CNS from immunological damage.
authors.affiliation: Department of Anatomy, Dankook University College of Medicine, Chonan, South Korea.
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Eunju
authors.initials: E
authors.lastname: Lee
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seonghan
authors.initials: S
authors.lastname: Kim
authors.forename: Sa Sun
authors.initials: SS
authors.lastname: Cho
authors.forename: Ka Young
authors.initials: KY
authors.lastname: Chang
authors.forename: Wang Jae
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D053222:Fas Ligand Protein; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger