title: [Selection of ergot alkaloid producers by induced mutagenesis].
abstract: Using the induced mutagenesis technique, A series of genetically modified Claviceps sp. VKM F-2609 strains that display high levels of agroclavine and elymoclavine synthesis were selected by induced mutagenesis. Compared to the parent strain, c106 displayed a 40-fold higher level of agroclavine synthesis, and c66 displayed an eightfold higher level of elymoclavine synthesis. The levels of synthesis of other alkaloids were decreased in these strains. The effects of various carbohydrates on the strain growth and ergot alkaloid biosynthesis was then investigated in both the parent strain and c106. The largest amount of agroclavine was synthesized by c106 strain growing on a medium with maltose.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.
authors.forename: I G
authors.initials: IG
authors.lastname: Vepritskaia
authors.forename: L V
authors.initials: LV
authors.lastname: Boĭchenko
authors.forename: M U
authors.initials: MU
authors.lastname: Arinbasarov
authors.forename: N F
authors.initials: NF
authors.lastname: Zelenkova
authors.forename: N V
authors.initials: NV
authors.lastname: Bobkova
mesh_terms: D002967:Claviceps / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D004873:Ergolines / Q000032:analysis / Q000378:metabolism; D004876:Ergot Alkaloids / Q000032:analysis / Q000096:biosynthesis*; D008320:Maltose / Q000378:metabolism; D016296:Mutagenesis; D015854:Up-Regulation
chemical_list: D003470:Culture Media; D004873:Ergolines; D004876:Ergot Alkaloids; C007586:elymoclavine; D008320:Maltose; C004465:agroclavine