[Effects of different cryoprotectants on biological properties of hematopoietic cells derived from cord blood].
OBJECTIVE
To search for effective cryoprotectants that can be used in cord blood bank to cryopreserve hematopoietic cells.


METHODS
Four kinds of combined reagents were used to cryopreserve cord blood cells. The effects of these cryoprotectants on biological properties of hematopoietic cells were monitored and compared after the cells were refrigerated for 1 to 4 months. The detected biological properties included the number of total nucleated cells (TNC), CD(34)(+) cells and CFCs.


RESULTS
The cryoprotectants of Dextran-40 + 10%DMSO resulted in the highest recovery rates, TNC, CD(34)(+) cells and CFCs were 77.8%, 78.5% and 87.2%, respectively. The combination of 10%DMSO + 6% AB serum + 5%HES led to the lowest recovery rates.


CONCLUSIONS
Dextran-40 + 10%DMSO is an ideal cryoprotectant combination for cryopreserving hematopoietic cells in the cord blood bank.
Chen
R
R
Baoan Blood Center and Cord Blood Bank, Shenzhen 518101, China.
Zhu
M
M
Huang
L
L
Liu
Y
Y
Xi
Y
Y
D018952:Antigens, CD34 / Q000032:analysis; D002452:Cell Count; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D013997:Time Factors
D018952:Antigens, CD34; D003451:Cryoprotective Agents; D003911:Dextrans; D004121:Dimethyl Sulfoxide