[Influence of a G/A substitution at -75 bp promoter region of apolipoprotein A I gene and wine consumption on the plasma apolipoprotein A I levels in Yi and Han Chinese].
OBJECTIVE
To study the influence of a common polymorphism of the human apolipoprotein A I gene promoter at a position of -75 bp upstream of the transcription start site and wine consumption on plasma lipid levels.


METHODS
742 healthy Yi and Han healthy subjects all above 15 years old to be the total population which was divided into three samples, namely, Yi-farmer sample, Yi-emigrant sample and Han-resident sample for this study. Estimations of plasma lipids and apolipoproteins were carried on through an auto-analyzer. Genetic DNA was prepared from the frozen blood clot using Triton x-100 lysis technique. Amplification of a 432 bp fragment of the apoA I gene promoter was performed via the PCR, followed by restriction digestion, electrophoresis and identification of the genotypes involved. Data analysis was done at last.


RESULTS
Four groups of alcohol consumption were defined: the non-drinkers, 1 - 25 g alcohol intake/day, 26 - 75 g alcohol/day and > 75 g alcohol/day. A tendency of a mild persistent elevation of plasma HDL-C and apoA I was noticed corresponding to an increase of the amount of alcohol intake, and with no evidence of threshold effect observed in the samples of both the Yi-farmers and the Han people. Similar phenomenon was obtained in the sample of Yi-emigrants, but no statistical significance. The frequencies of the A allele of all 3 samples were similar. In Han and Yi-emigrant samples, the drinkers with genotypes of GG had a higher plasma HDL-C and apoA I level than that of the non-drinkers with the same genotypes. Drinkers with A allele had a lower plasma HDL-C and apoA I level than that of drinkers without A allele, and the non-drinkers with A allele had a higher levels of apoA I than of non-drinkers with genotypes of GG. It is estimated that 18% of the variability of plasma apoA I level could be explained by the G/A polymorphism in non-drinkers of Yi-emigrants (F = 8.94, P < 0.01).


CONCLUSIONS
Current data suggest that a moderate alcohol consumption or a G to A substitution could make a lower incidence of CHD, but the beneficial effect of one will be negated by the other when both factors occur simultaneously. This finding is seemed valuable for a further study on the effect of the environmental factor or genetic factor in effecting the plasma apoA I level afterwards.
Zhao
Q
Q
Department of Pathology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100005, China.
Shen
P
P
Zhang
H
H
Zhou
M
M
She
M
M
D000293:Adolescent; D000328:Adult; D000428:Alcohol Drinking / Q000097:blood*; D016632:Apolipoprotein A-I / Q000097:blood / Q000235:genetics*; D002681:China; D008076:Cholesterol, HDL / Q000097:blood; D004247:DNA / Q000235:genetics / Q000378:metabolism; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*
D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D004247:DNA; D019003:Deoxyribonuclease HpaII