title: Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions.
abstract: We report that in vivo increased acetylation of the repressed Saccharomyces cerevisiae ADH2 promoter chromatin, as obtained by disrupting the genes for the two deacetylases HDA1 and RPD3, destabilizes the structure of the TATA box-containing nucleosome. This acetylation-dependent chromatin remodeling is not sufficient to allow the binding of the TATA box-binding protein, but facilitates the recruitment of the transcriptional activator Adr1 and induces faster kinetics of mRNA accumulation when the cells are shifted to derepressing conditions.
authors.affiliation: Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti and Centro di Studio per gli Acidi Nucleici, CNR, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, I-00185 Rome, Italy.
authors.forename: Loredana
authors.initials: L
authors.lastname: Verdone
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Wu
authors.forename: Kristen
authors.initials: K
authors.lastname: van Riper
authors.forename: Nataly
authors.initials: N
authors.lastname: Kacherovsky
authors.forename: Maria
authors.initials: M
authors.lastname: Vogelauer
authors.forename: Elton T
authors.initials: ET
authors.lastname: Young
authors.forename: Michael
authors.initials: M
authors.lastname: Grunstein
authors.forename: Ernesto
authors.initials: E
authors.lastname: Di Mauro
authors.forename: Micaela
authors.initials: M
authors.lastname: Caserta
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics*; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D008957:Models, Genetic; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D016385:TATA Box / Q000235:genetics*; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: C074682:ADR1 protein, S cerevisiae; D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D009707:Nucleosomes; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D000426:Alcohol Dehydrogenase; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D011494:Protein Kinases; C499673:HDA1 protein, S cerevisiae; C071665:RPD3 protein, S cerevisiae; D006655:Histone Deacetylases