[Clean-up procedure with ion-exchange mini column in the analysis of flusulfamide in agricultural products by HPLC].
A clean-up procedure with an ion-exchange column in the analysis of flusulfamide by HPLC was examined. Pesticide in the sample was extracted with methanol following liquid-liquid partition with ethyl acetate. The ethyl acetate fraction was cleaned up with silica gel column chromatography. The eluate from the silica gel column was further cleaned up with SAX + PSA mini column, then determined by HPLC. Carotenoids and interfering peaks were removed by washing the combined mini columns with 10 mL of 20% acetone-containing n-hexane and 5 mL of acetone, and flusulfamide was eluted with 35 mL of acetone.
Nakamura
Y
Y
National Institute of Health Sciences, Osaka Branch: 1-1-43, Hoenzaka, Chuo-ku, Osaka 540-0006, Japan.
Ishimitsu
S
S
Tsumura
Y
Y
Yoshii
K
K
Kaihara
A
A
Tonogai
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D018556:Crops, Agricultural / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007474:Ion Exchange; D010573:Pesticide Residues / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D013449:Sulfonamides
D010573:Pesticide Residues; D010575:Pesticides; D013449:Sulfonamides; C450479:flusulfamide