[Long-term expression of MHC-I gene in recipient mice].
OBJECTIVE
To explore the possibility of long-term expression of transferred MHC-I gene in recipient mice.


METHODS
MHC-I gene of the donor mouse (C(57)BL/6) was transferred into the recipient (BALB/c) hematopoietic cells by retrovirus-mediated gene transfer technique, and the gene transferred cells were reinfused into the recipient mice. PCR, RT-PCR and FACS were used to assay the long-term expression of MHC-I gene in the recipient mice.


RESULTS
The gene transferred mice hematopoietic cells and their G418-resistant CFU-GM were demonstrated to be able to express the MHC-I gene. The cells proliferation and differentiation had no change. The MHC-I gene could be expressed for a longer period in the recipient mice.


CONCLUSIONS
This experiment makes ready for the further research on inducing transplantation tolerance through MHC-I gene transfer.
Li
Z
Z
Laboratory of Surgery, The First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China.
Li
S
S
Xu
H
H
Zeng
W
W
Chen
G
G
Zhu
Z
Z
D000818:Animals; D003114:Colony-Forming Units Assay; D005260:Female; D015870:Gene Expression; D006183:H-2 Antigens / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012190:Retroviridae / Q000235:genetics; D013997:Time Factors; D014162:Transfection; D023001:Transplantation Tolerance / Q000235:genetics; D014184:Transplantation, Homologous
D006183:H-2 Antigens