[Transformation of human bronchial epithelium by EB virus gene LMP1 and EBNA2].
OBJECTIVE
To observe Epstein-Barr virus (EBV)-dependent transformation of human bronchial epithelium.


METHODS
Eukaryotic expression plasmide pBS-LMP-Hyg and pSG5-EBNA2-Hyg were transfected into immortalized human bronchial epithelium cell line TR.


RESULTS
In situ hybridization and Western blot confirmed EBNA2 and LMP1 expression in one hygromycine B resistant cell clone TR/LMP1-EBNA2. Growth curve and MTT value showed that transformed cells grew faster. Soft agar plating rate of TR/LMP1-EBNA2 was 50.1per thousand, 68.2per thousand, empty vector PBs-Hyg transfected cell strain TR/Hyg 2.0per thousand, 3.0per thousand, TR 11.0per thousand, 0.7per thousand.


CONCLUSIONS
Expression of both LMP1 and EBNA2 led to decreased anchorage dependence and increased proliferation rate of TR, thereby further transformed immortalized human bronchial epithelium. Our results suggest that EBV may play a role in genesis of lung cancer.
Zhang
L
L
Department of Pathology, Peking Union Hospital, Chinese Academy of Medical sciences and Peking Union Medical College, Beijing 100730.
Liu
H
H
Liu
T
T
Cheng
S
S
D000818:Animals; D015153:Blotting, Western; D001980:Bronchi / Q000166:cytology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D002461:Cell Line, Transformed / Q000166:cytology / Q000378:metabolism*; D017690:Cell Transplantation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection; D014183:Transplantation, Heterologous; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins
C402641:EBNA-2 protein, Human herpesvirus 4; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D014763:Viral Matrix Proteins; D014764:Viral Proteins