[Effect of glycogen on calcium of donor liver during ischemia-reperfusion period].
OBJECTIVE
To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.


METHODS
Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.


RESULTS
Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.


CONCLUSIONS
Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.
Wang
Yu
Y
PLA Center of General Surgery, General Hospital of Chengdu Military Command, Chengdu 610083, China.
Tian
Fuzhou
F
Tang
Lijun
L
Huang
Darong
D
Li
Xiaojun
X
Yin
Zhiliang
Z
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D003600:Cytosol / Q000737:chemistry; D005260:Female; D006003:Glycogen / Q000378:metabolism*; D008107:Liver Diseases / Q000201:enzymology / Q000378:metabolism*; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D011817:Rabbits; D015427:Reperfusion Injury / Q000201:enzymology / Q000378:metabolism*
D000255:Adenosine Triphosphate; D006003:Glycogen; D000252:Calcium-Transporting ATPases; D002118:Calcium