Methods for the analysis of protein-chromatin interactions.
The analysis of protein interactions with chromatin is vital for the understanding of DNA sequence recognition in vivo. Chromatin binding requires the interaction of proteins with DNA lying on the macromolecular protein surface of nucleosomes, a situation that can alter factor binding characteristics substantially when compared with naked DNA. It is therefore important to study these protein-DNA interactions in the context of a chromatin substrate, the more physiologically relevant binding situation. In this article we review techniques used in the investigation of protein interactions with defined nucleosomal templates.
Brickwood
Sarah J
SJ
Sangamo Biosciences Inc., Point Richmond Tech Center, Richmond, CA 04804, USA.
Myers
Fiona A
FA
Chandler
Simon P
SP
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D009865:Oocytes; D011485:Protein Binding / Q000502:physiology; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism*; D014981:Xenopus
D002843:Chromatin; D006657:Histones; D014157:Transcription Factors