Deoxyhemoglobin-mediated lipid oxidation in washed fish muscle.
Deoxyhemoglobin-mediated lipid oxidation was studied by comparing the pro-oxidative activity of anodic and cathodic hemoglobins from trout in a washed cod muscle model system. At pH 6.3, cathodic hemoglobins were nearly fully oxygenated while anodic hemoglobins were poorly oxygenated. Anodic hemoglobins initiated lipid oxidation in washed cod muscle much more rapidly than cathodic hemoglobins, as measured by thiobarbituric acid reactive substances (TBARS) formation. Moreover, anodic hemoglobins appeared to oxidize more rapidly as compared to cathodic hemoglobins in the washed cod muscle model system, as measured by a decrease in redness (a value). A more pronounced pro-oxidative activity of deoxyhemoglobin as compared to oxyhemoglobin was confirmed by accelerated lipid hydroperoxide and TBARS formation in the washed cod muscle model system upon combined addition of anodic hemoglobins and adenosine triphosphate, which is known to lower the oxygenation of anodic hemoglobins at pH 7.2, as compared to only addition of anodic hemoglobins to the washed cod muscle. These studies suggest that deoxyhemoglobin is more pro-oxidative than its oxygenated counterpart at pH values found in postmortem fish muscle.
Richards
Mark P
MP
Muscle Biology and Meat Science Laboratory, University of Wisconsin-Madison, Madison, WI 53706-1284, USA. mprichards@facstaff.wisc.edu
Østdal
Henrik
H
Andersen
Henrik J
HJ
D000818:Animals; D006454:Hemoglobins / Q000378:metabolism*; D006461:Hemolysis; D007700:Kinetics; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D017686:Oncorhynchus mykiss; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D013053:Spectrophotometry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
D006454:Hemoglobins; D008054:Lipid Peroxides; D010108:Oxyhemoglobins; D017392:Thiobarbituric Acid Reactive Substances; C100003:deoxyhemoglobin