title: [Mutation BRCA1 gene in 186 breast cancer patients].
abstract: OBJECTIVE
  To analysis the mutation of BRCA1 gene in 186 breast cancer patients.
  
  
  METHODS
  Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and sequential were used in this analysis.
  
  
  RESULTS
  13/186 of patients showed mutation which comprised 7% of the total number of patients. In 8 patients, the mutation was observed in the intron splicing region and in 5 patients, the mutation was on the exon.
  
  
  CONCLUSIONS
  Mutation analysis of BRCA1 gene is helpful in the determination of developmental potential, early diagnosis and gene therapy for breast cancer.
authors.affiliation: Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: C
authors.initials: C
authors.lastname: Lai
authors.forename: Z
authors.initials: Z
authors.lastname: Jiang
authors.forename: S
authors.initials: S
authors.lastname: Song
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D017422:Sequence Analysis, DNA