Hydrolytic activity and ultrastructural changes in fruit skins from two prickly pear (Opuntia sp.) varieties during storage.
The activity of four cell wall hydrolases, pectinmethylesterase (PME), polygalacturonase (PG), cellulase, and beta-galactosidase (beta-Gal), was measured in fruit skins of two prickly pear varieties, Naranjona and Charola, during storage at 18 degrees C and 85-95% relative humidity (RH). In Naranjona (Opuntia ficus indica), of short postharvest life (ca. 2 weeks), PG, cellulase, and beta-Gal increased their activity more than twice, whereas PME activity tended to increase only slightly during storage. In Charola (Opuntia sp.), of long postharvest life (ca. 2 months), only beta-Gal increased its activity (77%), showing a high PG activity from the beginning of storage. Transmission electron microscopy observations showed middle lamella dissolution at the end of storage for both varieties. Naranjona showed a higher cell wall enzymatic activity than Charola, in agreement with their storability differences. Our results suggest that PG and cellulase in Naranjona and PG and beta-Gal in Charola are the main enzymes responsible for cell wall hydrolytic and ultrastructural changes in skins of stored prickly pears.
Carrillo-López
Armando
A
Departamento de Biotecnología y Bioquímica, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Libramiento Norte Km 9.6, Carretera Irapuato-León, Apdo. Postal 629, 36500 Irapuato, Gto., México.
Cruz-Hernández
Andrés
A
Cárabez-Trejo
Alfonso
A
Guevara-Lara
Fidel
F
Paredes-López
Octavio
O
D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D002473:Cell Wall / Q000201:enzymology / Q000648:ultrastructure; D002480:Cellulase / Q000032:analysis; D005519:Food Preservation; D005638:Fruit / Q000201:enzymology* / Q000648:ultrastructure; D006867:Hydrolases / Q000032:analysis*; D008854:Microscopy, Electron; D029747:Opuntia / Q000201:enzymology*; D011096:Polygalacturonase / Q000032:analysis; D013997:Time Factors; D001616:beta-Galactosidase / Q000032:analysis
D006867:Hydrolases; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase; D001616:beta-Galactosidase; D002480:Cellulase