Differences between the prion protein and its homolog Doppel: a partially structured state with implications for scrapie formation.
The key event in the pathogenesis of prion diseases is a conformational change in the prion protein (PrP). Models for conversion of PrP(C) into PrP(Sc) typically implicate an, as yet, unidentified intermediate. In an attempt to identify such an intermediate, we used native-state hydrogen exchange monitored with NMR. Although we were unable to detect an intermediate directly, we observed substantial protection above that expected based upon measurements of the global stability of PrP (>2 kcal mol(-1) super protection). This super protection implicates either structure in the denatured state or the presence of an intermediate. Similar experiments with Doppel, a homolog of PrP that does not form infectious prions, failed to demonstrate such super protection. This suggests that the partially structured state of PrP encompassing portions of the B and C helices, may be a significant factor in the ability of PrP to convert from PrP(C) to PrP(Sc).
Nicholson
Eric M
EM
Department of Molecular & Cell Biology, University of California, 229 Stanley Hall, Berkeley, CA 94720, USA.
Mo
Huaping
H
Prusiner
Stanley B
SB
Cohen
Fred E
FE
Marqusee
Susan
S
D000595:Amino Acid Sequence; D000818:Animals; D002942:Circular Dichroism; D006224:Cricetinae; D058851:GPI-Linked Proteins; D006859:Hydrogen / Q000378:metabolism; D008647:Mesocricetus; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D012608:Scrapie / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
D058851:GPI-Linked Proteins; D018620:PrPSc Proteins; D011328:Prions; C122036:Prnd protein, mouse; D006859:Hydrogen; D014508:Urea