Galectin fingerprinting by immuno- and lectin histochemistry in cutaneous lymphoma.
Owing to their relevance for growth regulation and cell adhesion monitoring the expression of galectins (endogenous beta-galactoside-binding lectins) and their binding sites has relevance for tumor biology. Using galectin-type-specific reagents (non-crossreactive antibodies for proto-type galectin-1, chimera-type galectin-3 and tandem-repeat-type galectins-4 and -8, and labeled galectins-1, -3, and -4) we determined galectin expression in cutaneous T cell lymphomas (CTCL) and controls. In addition to commonly studied galectins-1 and -3, tandem-repeat-type galectins could be detected, i.e., galectin-8 in six from 15 cases and galectin-4 in one of 15 cases. In view of relevant ligands such as bcl-2 or integrins the presence of galectins-3 and -8 seems to be possibly related to loss of proliferation control and change in cell adhesion properties that are involved in clonal expansion and epidermal spread of malignant T cell clones. Successful chemotherapy of CTCL alters galectin expression selectively as shown for liposomal doxorubicin.
Wollina
U
U
Department of Dermatology and Dermatological Allergology, Friedrich-Schiller-University, Jena, Germany. Wollina-Uw@khdf.de
Graefe
T
T
Feldrappe
S
S
André
S
S
Wasano
K
K
Kaltner
H
H
Zick
Y
Y
Gabius
H-J
HJ
D000368:Aged; D000906:Antibodies; D000970:Antineoplastic Agents / Q000494:pharmacology; D001665:Binding Sites; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D004317:Doxorubicin / Q000494:pharmacology; D005260:Female; D037161:Galectins; D015972:Gene Expression Regulation, Neoplastic; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D008081:Liposomes; D016410:Lymphoma, T-Cell, Cutaneous / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis
D000906:Antibodies; D000970:Antineoplastic Agents; D037161:Galectins; D006388:Hemagglutinins; D008024:Ligands; D008081:Liposomes; D019253:Proto-Oncogene Proteins c-bcl-2; D004317:Doxorubicin