title: A flavonoid-rich diet increases nitric oxide production in rat aorta.
abstract: 1. Red wine intake is associated with a low risk of cardiovascular disease. This effect has been partly attributed to the action of polyphenolic compounds, which decrease the oxidation of plasma low density lipoproteins. Moreover, nitric oxide ((*)NO) is a vasodilator and polyphenolic compounds induce endothelium-dependent vasorelaxation in vitro. 2. Here we studied whether a diet rich in dealcoholated red wine (DRW) increases acetylcholine-induced vasorelaxation and whether ingestion of DRW-, quercetin- or catechin-rich diets modifies the (*)NO-cyclic guanosine-3',5'-monophosphate (cyclic GMP) pathway and superoxide anion (O2(.-)) release in aorta in a resting state in rats fed semi-purified diets containing either 35% (v w(-1)) DRW, 0.3% (w w(-1)) quercetin or 0.3% (w w(-1)) catechin for 10 days. 3. (*)NO-mediated vasorelaxation induced by acetylcholine was greater in rats fed the DRW-rich diet than in those that received the control diet. 4. Expression of endothelial (*)NO synthase (eNOS) was similar in the four dietary groups. The aortic rings of rats fed either the DRW-, quercetin-, or catechin-rich diets showed higher NOS activity, (*)NO production and cyclic GMP content than those of rats fed the control diet. No changes were observed in O2(.-) production. 5. In summary, diets rich in either DRW, quercetin or catechin induced endothelium-dependent vasorelaxation in rat aorta in a resting state through the enhancement of (*)NO production, without modifying O2(.-) generation, thus the bioavailability of (*)NO was increased. The increase in the (*)NO-cyclic GMP pathway explains the beneficial effect of flavonoids at vascular level.
authors.affiliation: Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Benito
authors.forename: D
authors.initials: D
authors.lastname: Lopez
authors.forename: M P
authors.initials: MP
authors.lastname: Sáiz
authors.forename: S
authors.initials: S
authors.lastname: Buxaderas
authors.forename: J
authors.initials: J
authors.lastname: Sánchez
authors.forename: P
authors.initials: P
authors.lastname: Puig-Parellada
authors.forename: M T
authors.initials: MT
authors.lastname: Mitjavila
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism* / Q000502:physiology; D002392:Catechin / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism; D004032:Diet; D004730:Endothelium, Vascular / Q000378:metabolism; D000431:Ethanol / Q000032:analysis; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D010636:Phenols / Q000032:analysis; D011108:Polymers / Q000032:analysis; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology; D014920:Wine
chemical_list: D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D014665:Vasodilator Agents; D013481:Superoxides; D009569:Nitric Oxide; D000431:Ethanol; D002392:Catechin; D011794:Quercetin; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D006152:Cyclic GMP; D000109:Acetylcholine