[Positive immunoglobulin A expression in human epithelial carcinoma cell lines].
OBJECTIVE
In the present study, we try to further elucidate which class of immunoglobulin is expressed in epithelial carcinoma cell lines.


METHODS
Three assays, immunohistochemistry, Western blot and enzyme-linked immunosorbent assay (ELISA) were used to detect the cellular protein extracts and the cultivated supernatant.


RESULTS
Seven human epithelial carcinoma cell lines, MCF-7 (human breast carcinoma cell line), SW480 (human colon carcinoma cell line), MGC (human gastric carcinoma cell line), HeLa (human cervical carcinoma cell line), HNE2, CNE1-LMP1 and Tet-on-LMP1-HNE2 (three human nasopharyngeal carcinoma cells) expressed IgA protein in their cellular extracts and cultivated supernatants.


CONCLUSIONS
Immunoglobulin is expressed in epithelium-derived carcinoma cell lines, which is in conflict with the traditional immunoglobulin theory. Our findings imply that the immunoglobulin expressed in epithelial carcinoma cells might play a role in the carcinogenesis of epithelium-derived carcinoma. Understanding the mechanism of immunoglobulin expression by cancer cells may possibly further facilitate the improvement techniques in cancer diagnosis, therapy or the mechanism of carcinogenesis.
Li
M
M
Cancer Institute, Hunan Medical University, Changsha 410078, China.
Tang
M
M
Deng
X
X
D003470:Culture Media; D004847:Epithelial Cells / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000276:immunology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D007070:Immunoglobulin A