[Involvement of mitochondrial membrane potential in the homoharringtonine induced apoptosis of leukemic T-cells].
OBJECTIVE
Investigation of the role of mitochondrial membrane potential (MMP) in the homoharringtonine (HHT)-induced apoptosis.


METHODS
Annexin V staining, flow cytometry and confocal laser scan microscopy were used to observe the relationship between Bax, cytochrome C and MMP in the HHT-induced apoptosis of leukemic T lymphocytic line Molt-3.


RESULTS
The induction of apoptosis by HHT resulted in the translocation of Bax from cytosol to mitochondrial membrane and the decrease of cellular MMP, followed by the release of cytochrome C from mitochondria to cytosol.


CONCLUSIONS
Changes of mitochondrial membrane potential might play a critical role in the HHT-induced apoptosis of leukemic T-cells.
Cai
Z
Z
Department of Hematology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
Lin
M
M
Ludwig
W D
WD
Karawajew
L
L
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006248:Harringtonines / Q000494:pharmacology*; D000077863:Homoharringtonine; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000503:physiopathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051336:Mitochondrial Membranes / Q000378:metabolism; D021381:Protein Transport; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D006248:Harringtonines; D051028:bcl-2-Associated X Protein; D000077863:Homoharringtonine; D045304:Cytochromes c