[Effects of EB virus LMP on the differentiation and growth of human nasopharyngeal carcinoma cell line CNE1].
OBJECTIVE
To study the effects of EBV-LMP gene on differentiation and growth of human nasopharyngeal carcinoma (NPC) cell.


METHODS
With NPC cell line (CNE1) as target, electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells. The proliferation changes of transfectants were measured in vitro by proliferation experiment. FCM method and transplantation in nude mice were used.


RESULTS
LMP promoted the growth ability of NPC CNE1 cells in vitro. The average A ratio (3.98 +/- 0.11) in the treatment group was higher than in the control group (2.36 +/- 0.05) and also in the negative control group (2.75 +/- 0.07, P < 0.01). Clone forming test (25.2%, 378/1 500) in the treatment group was higher than in the control groups (11.2%, 168/1 500) and negative control groups (13.4%, 201/1 500). When FCM method was used, the keratin positive rate (82.7%) in the treatment group was markedly decreased as compared with the control (92.5%) and negative control groups (95.7%, P < 0. 05). Latent period (26.6 +/- 7.7) days and internal double time (2.51 +/- 0.18) days of transplant tumor of the treatment group were markedly shortened, tumor take rate (5/6) was increased, cells of the transplant tumor in the treatment group showed an image of small size, great allotype, and a trend of low differentiation.


CONCLUSIONS
The EBV-LMP can promote the growth and inhibit the differentiation of NPC CNE1 cells. EBV-LMP may play an important role in the development of NPC and in studying its mechanism.
Zhang
Q
Q
Department of Pathology, Guangdong Medical College, Zhanjiang 524023.
Sun
N
N
Chen
X
X
Mo
M
M
Li
F
F
Cai
Q
Q
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D013997:Time Factors; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000502:physiology
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins