Highly sensitive chemiluminescence detection of copper(II) in capillary electrophoresis with field-amplified sample injection.
Field-amplified sample injection of copper(II) was investigated using capillary electrophoresis with chemiluminescence detection. The sensitivity of copper(II) has been improved markedly by the field-amplified sample injection technique and the detection limit reaches 2 x 10(-11) M. By injection of a short plug of water before sample introduction, the sensitivity can be further improved 5-fold and the detection limit reaches 4 x 10(-12) M. The relative standard deviations (n = 6) of the migration time and the peak height are 0.61% and 4.7% at 1.0 x 10(-9) M Cu(II), respectively. Parameters affecting the field-amplified sample injection, such as separation voltage and concentration of electrophoretic buffer, have been investigated.
Liu
Yan-Ming
YM
Department of Chemistry, Wuhan University, Wuhan, China.
Cheng
Jie-Ke
JK
D003300:Copper / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D008163:Luminescent Measurements; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003300:Copper