Quantitative determination of the dual COX-2/5-LOX inhibitor tryptanthrin in Isatis tinctoria by ESI-LC-MS.
Isatis tinctoria L. is an old European and Chinese dye plant and anti-inflammatory herb from which the potent cyclooxygenase-2 and 5-lipoxygenase inhibitor tryptanthrin (1) (indolo-[2,1-b]-quinazoline-6,12-dione) was recently isolated as one of the active principles. An HPLC method for the quantitative analysis of the compound in plant material was developed. Reproducible extraction was achieved by accelerated solvent extraction (ASE). Detection by UV at 254 and 387 nm and by electrospray-MS were compared. The low tryptanthrin content in the herb and possible interferences required isocratic high-performance liquid chromatography coupled with electrospray-MS in single ion mode. More than 70 Isatis samples of different origin were analyzed. The tryptanthrin content in leaf samples varied from 0.56 to 16.74 x 10(-3) %.
Danz
Henning
H
Institute of Pharmacy, Friedrich-Schiller-University, Jena, Germany.
Baumann
Dietmar
D
Hamburger
Matthias
M
D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D019607:Brassicaceae; D002851:Chromatography, High Pressure Liquid; D051546:Cyclooxygenase 2; D004365:Drugs, Chinese Herbal; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011799:Quinazolines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013057:Spectrum Analysis
D004365:Drugs, Chinese Herbal; D007527:Isoenzymes; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D011799:Quinazolines; C046243:tryptanthrine; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases