A simple and rapid assay of collagen-like polymer in crude lysate from Escherichia coli.
An assay for the quantification of collagen-like polymer (CLP) in Escherichia coli cells utilizing the specific reaction between collagenase and CLP is presented. It involves thermal treatment to precipitate non-CLP proteins, digestion of CLP by collagenase and detection of the absorbance of the liberated amino acids and peptides from CLP by a ninhydrin-based method. CLP concentration is determined from the absorbance measurement.
Yin
Jin
J
Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Building 16, Room 429, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Tomycz
Luke
L
Bonner
Gary
G
Wang
Daniel I C
DI
D003094:Collagen / Q000032:analysis*; D003124:Colorimetry; D004926:Escherichia coli / Q000737:chemistry* / Q000201:enzymology; D003012:Microbial Collagenase / Q000378:metabolism; D009555:Ninhydrin; D011108:Polymers / Q000032:analysis*
D011108:Polymers; D003094:Collagen; D003012:Microbial Collagenase; D009555:Ninhydrin