Genetic diversity among Geotrichum candidum strains from various substrates studied using RAM and RAPD-PCR.
OBJECTIVE
Assessment of genetic diversity within the species Geotrichum candidum and development of tools to trace the strains that play an important role in the agro food industry.


RESULTS
RAM-PCR and RAPD-PCR techniques were assessed for their ability to discriminate 57 strains of various morphotypes, substrates and geographical origin. The techniques were complementary and, when combined, allowed us to discriminate isolates. Moreover, we established a link between a taxon and its occupation of an ecological niche, which should not be confused with the substrate of isolation.


CONCLUSIONS
We observed a high degree of diversity, which could be linked to the variety of the ecological niches chosen and to the high degree of morphological polymorphism encountered within the species.


CONCLUSIONS
Used in combination, RAM-PCR and RAPD-PCR permit traceability and monitoring systems for G. candidum strains during food processing.
Gente
S
S
Laboratoire de Microbiologie Alimentaire EA 3213, Institut de Biochimie et Biologie Appliquée, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France.
Desmasures
N
N
Panoff
J-M
JM
Guéguen
M
M
D000818:Animals; D002417:Cattle; D004271:DNA, Fungal / Q000032:analysis; D004783:Environmental Microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D014644:Genetic Variation; D005848:Geotrichum / Q000145:classification* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal