title: [Study on structure change and hypermethylation of p16 gene in multiple myeloma].
abstract: OBJECTIVE
  To illustrate the role of structure and hypermethylation of p16 gene in the pathogenesis of multiple myeloma(MM).
  
  
  METHODS
  By using PCR-single strand conformation polymorphisms(PCR-SSCP) and methylation-specific PCR(MSP) techniques, the structure and hypermethylation status of p16 gene in MM cell lines and patients were analysed.
  
  
  RESULTS
  Homozygous deletion of p16 exon 2 was found in KM3 cells. The completely methylated p16 gene and hypermethylation of CPG island were observed in U266, LP1 cell lines and 55.56% of MM patients.
  
  
  CONCLUSIONS
  Methylation of p16 gene is important in the pathogenesis of MM and may provide a new drug target for the treatment of MM.
authors.affiliation: Department of Hematology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: W
authors.initials: W
authors.lastname: Fu
authors.forename: J
authors.initials: J
authors.lastname: Hou
authors.forename: D
authors.initials: D
authors.lastname: Wang
mesh_terms: D000368:Aged; D018899:CpG Islands / Q000235:genetics*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D005091:Exons; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16