Sperm aneuploidy in fathers of children with paternally and maternally inherited Klinefelter syndrome.
BACKGROUND
It is unclear whether frequency of sperm aneuploidy is associated with risk of fathering children with trisomy.


METHODS
We recruited 36 families with a boy with Klinefelter syndrome (KS), interviewed the fathers about their exposures and medical history, received a semen sample from each father, and collected blood samples from the mother, father and child. We applied a multicolour fluorescent in-situ hybridization assay to compare the frequencies of sperm carrying XY aneuploidy and disomies X, Y and 21 in fathers of maternally and paternally inherited KS cases.


RESULTS
Inheritance of the extra X chromosome was paternal in 10 and maternal in 26 families. Fathers of paternal KS cases produced higher frequencies of XY sperm (P = 0.02) than fathers of maternal KS cases. After controlling for age, the major confounding variable, the difference between the two groups was no longer significant (P less-than-or-equal 0.2). Also, there were no significant differences between the parental origin groups for disomy X, Y or 21.


CONCLUSIONS
Men who fathered a child with a Klinefelter syndrome produced higher frequencies of XY sperm aneuploidy, which is explained, in part, by both paternal age and parent of origin.
Eskenazi
B
B
School of Public Health, University of California, Berkeley, CA 94720, USA. eskenazi@uclink4.berkeley.edu
Wyrobek
A J
AJ
Kidd
S A
SA
Lowe
X
X
Moore
D
D
Weisiger
K
K
Aylstock
M
M
D000328:Adult; D000782:Aneuploidy; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D005220:Fathers; D006801:Humans; D007713:Klinefelter Syndrome / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009035:Mothers; D010331:Paternal Age; D012044:Regression Analysis; D013094:Spermatozoa / Q000502:physiology*; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics