[Expression of membrane-type I matrix metalloproteinase and its correlation with gelatinase A activation in cancer cell lines].
OBJECTIVE
To study the expression of membrane-type I matrix metalloproteinase (MT1-MMP) and its correlation with gelatinase A (MMP2) activation.


METHODS
Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1-MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines.


RESULTS
All the cancer cell lines expressed MT1-MMP mRNA, and the MT1-MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines. Activated MMP2 proteins were only detected in the melanoma cell lines, whereas PG, a lung cancer cell line, which secreted pro-MMP2 and expressed low level of MT1-MMP, could not produce activated MMP2.


CONCLUSIONS
The level of MT1-MMP expression was highly associated with MMP2 activation.
Gao
Q
Q
Department of Pathology, Beijing Medical University, Beijing 100083.
Wu
B
B
Zhang
J
J
You
J
J
Zhong
H
H
Fang
W
W
D015152:Blotting, Northern; D015153:Blotting, Western; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2