Analysis of amlodipine in human plasma by liquid chromatography-mass spectrometry.
A simple, sensitive, and specific liquid chromatographic method coupled with electrospray ionization (ESI)-mass spectrometry for the determination of amlodipine is developed. After extraction by ethyl acetate using nicardipine as the internal standard, solutes are separated on a C18 column with a mobile phase of methanol-1% HAc (65:35). Detection is performed on an air pressure ionization single quadruple mass spectrometer equipped with an ESI interface and operated in positive-ionization mode. Amlodipine quantitation is realized by computing the peak-area ratio (amlodipine-nicardipine) of the extracts analyzed in single ion monitoring mode (m/z 431 and m/z 480 for amlodipine and nicardipine, respectively) and comparing them with the calibration curve (r = 0.9991).
Feng
Yan
Y
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, People's Republic of China. purplefeng2000@yahoo.com
Zhang
Liang
L
Shen
Zheng
Z
Pan
Fengyun
F
Zhang
Zhengxing
Z
D017311:Amlodipine / Q000097:blood*; D002121:Calcium Channel Blockers / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002121:Calcium Channel Blockers; D017311:Amlodipine