Distribution of ClC-2 chloride channel in rat and human epithelial tissues.
The ubiquitous ClC-2 Cl(-) channel is thought to contribute to epithelial Cl(-) secretion, but the distribution of the ClC-2 protein in human epithelia has not been investigated. We have studied the distribution of ClC-2 in adult human and rat intestine and airways by immunoblotting and confocal microscopy. In the rat, ClC-2 was present in the lateral membranes of villus enterocytes and was predominant at the basolateral membranes of luminal colon enterocytes. The expression pattern of ClC-2 in the human intestine differed significantly, because ClC-2 was mainly detected in a supranuclear compartment of colon cells. We found significant expression of ClC-2 at the apex of ciliated cells in both rat and human airways. These results show that the distribution of ClC-2 in airways is consistent with participation of ClC-2 channels in Cl(-) secretion and indicate that extrapolation of results from studies of ClC-2 function in rat intestine to human intestine is not straightforward.
Lipecka
Joanna
J
Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 467, Faculté de Médecine Necker-Enfants Malades, 75015 Paris, France.
Bali
Moëz
M
Thomas
Annick
A
Fanen
Pascale
P
Edelman
Aleksander
A
Fritsch
Janine
J
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D003106:Colon / Q000737:chemistry; D020895:Enterocytes / Q000737:chemistry; D015870:Gene Expression / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry*; D007421:Intestine, Small / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D020545:Respiratory Mucosa / Q000737:chemistry*
D000906:Antibodies; D000090007:CLC-2 Chloride Channels; D018118:Chloride Channels