Modulation of neutrophil apoptosis by plasma and peritoneal fluid from patients with advanced endometriosis.
BACKGROUND
The increased production of pro-inflammatory chemoattractant cytokines for neutrophils in endometriosis suggests that changes in the immune system play an important role in the pathophysiology of endometriosis. The effects of plasma and peritoneal fluid from patients with advanced endometriosis on the apoptosis of neutrophils were investigated.


METHODS
Apoptotic changes of neutrophils were evaluated by morphological changes using Giemsa staining. Apoptosis was confirmed by DNA electrophoretic analysis.


RESULTS
Compared with the plasma (n = 20) and peritoneal fluid (n = 5) of healthy controls, the addition of 10% plasma (n = 20) and peritoneal fluid (n = 10) from patients with endometriosis to an in-vitro culture of neutrophils from healthy subjects reduced the percentage of apoptotic cells from 65.3 +/- 6.6 to 27.2 +/- 4.6% (P < 0.001) and from 45.3 +/- 4.8 to 10.5 +/- 4.3% (P < 0.001) respectively. Neutralizing interleukin-8 antibody abrogated the delay of neutrophil apoptosis induced by peritoneal fluid, but not in the plasma of endometriosis patients.


CONCLUSIONS
These findings show that interleukin-8 is one of the neutrophil survival factors in the peritoneal fluid of endometriosis patients and that an unidentified survival factor is also present in the plasma of patients with endometriosis.
Kwak
Jong-Young
JY
Dong-A University Institute of Medical Science, Pusan 602-103, Korea.
Park
Sang-Won
SW
Kim
Ki-Hyung
KH
Na
Yong-Jin
YJ
Lee
Kyu-Sup
KS
D000328:Adult; D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001202:Ascitic Fluid / Q000503:physiopathology*; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D003609:Dactinomycin / Q000494:pharmacology; D004715:Endometriosis / Q000503:physiopathology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012016:Reference Values; D013997:Time Factors
D000906:Antibodies; D016209:Interleukin-8; D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin