[Induction of quinone reductase and glutathion-s-transferase by dimethyl fumarate in rats].
OBJECTIVE
To assess induction of quinone reductase (QR) and glutathion-s-transferases (GSTs) by dimethyl fumarate (DMF) in major viscera of rats.


METHODS
Fodder fed to male Wistar rats was supplemented with 2.0% DMF, and blood specimens were collected form the rats 6 h, 24 h, 72 h, 1 week and 2 weeks after feeding, respectively, and then animals were killed. QR and GSTs activities were determined in rats' gland stomach, liver, lung, kidney and heart, and also QR, LDH and GPT activities in their sera were measured with enzyme dynamic methods.


RESULTS
Administration of DMF for 24 h, 72 h, 1 week and 2 weeks in rats can significantly increase activities of QR and GSTs in their gland stomach, liver, lung and kidney, as compared with those in controls (P < 0.0001, or P < 0.01), and their serum QR also significantly increased (P < 0.0001, or P < 0.01). But, DMF had no effect on activities of serum GPT and LDH (P > 0.05).


CONCLUSIONS
DMF is a potent induction agent for QR and GSTs in the gland stomach, liver, lung and kidney of rats, which suggests it will hopefully be a protective and antidotic agent against human antioxidant injury.
Wang
L
L
Digestive Diseases Research Center, Beijing Medical University, Beijing 100083.
Lin
S
S
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D004126:Dimethylformamide / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016588:Anticarcinogenic Agents; D004126:Dimethylformamide; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase