The role of kinin B1 in the plasma extravasation of carrageenin-induced pleurisy.
The role of des-Arg9-bradykinin (des-Arg9-BK) and kinin B1 receptor in the plasma extravasation of rat carrageenin-induced pleurisy was investigated employing B1 receptor agonist and antagonists and kininogen-deficient rats. Expression of the B1 receptor mRNA in pleura was induced from 3 to 5 h after the injection of carrageenin into the pleural cavity of Sprague-Dawley rats. Exogenous injection of des-Arg9-BK into the pleural cavity provoked a significant increase in plasma extravasation in 5 h carrageenin-induced pleurisy, but not in 20 min kaolin-induced pleurisy. The level of immunoreactive des-Arg9-BK in the exudate of 5 h carrageenin-induced pleurisy was higher than that of bradykinin (BK). Administration of the B1 receptor antagonists, des-Arg9-[Leu8]-BK or des-Arg9-D-Arg-[Hyp3, Thi5, D-Tic7,Oic8]-BK significantly reduced the exudation rate. However, intrapleural administration of des-Arg9-BK to plasma kininogen-deficient. Brown Norway-Katholiek rats did not result in a further increase in the plasma extravasation. In conclusion, endogenously generated des-Arg9-BK could contribute to the plasma extravasation in carrageenin-induced pleurisy via mediation of the inducible B1 receptor.
Hayashi
Izumi
I
Department of Pharmacology, Kitasato University School of Medicine, Sagamihara-shi, Kanagawa, Japan. hayashii@med.kitasato-u.ac.jp
Amano
Hideki
H
Ishihara
Keiko
K
Kumagai
Yuji
Y
Yoshimura
Hirokuni
H
Majima
Masataka C
MC
D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000633:toxicity*; D065168:Bradykinin Receptor Antagonists; D002351:Carrageenan / Q000494:pharmacology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007616:Kaolin / Q000494:pharmacology; D007704:Kininogens / Q000494:pharmacology; D008297:Male; D051379:Mice; D010949:Plasma / Q000378:metabolism*; D010994:Pleura / Q000166:cytology; D010998:Pleurisy / Q000139:chemically induced* / Q000188:drug therapy / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin / Q000819:agonists / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000930:Antidiarrheals; D065168:Bradykinin Receptor Antagonists; D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens; D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin; C024626:bradykinin, des-Arg(9)-; D007616:Kaolin; D002351:Carrageenan; D001920:Bradykinin