title: In vivo GABA editing using a novel doubly selective multiple quantum filter.
abstract: A novel multiple quantum filtering method is proposed that uses a doubly selective pulse termed Delays Alternating with Nutations for Tailored Excitation (DANTE) for multiple quantum preparation. This method selectively prepares GABA-3 and GABA-4 into a multiple quantum state and suppresses all other resonances at 3.0 ppm in each single scan. Phantom tests demonstrated excellent GABA signal retention and complete suppression of overlapping metabolites. It is shown using numerical simulations that overlapping macromolecules are suppressed because the frequency of the first upfield 2pi rotation of the doubly selective DANTE pulse coincides with that of the macromolecules at 1.72 ppm. Excellent suppression of overlapping macromolecules was demonstrated in vivo. Using this method the concentration of GABA in the occipital lobe of healthy volunteers was measured to be 1.21 +/- 0.28 micromol/mL (mean +/-SD, N = 9).
authors.affiliation: Center for Advanced Brain Imaging, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, New York 10962, USA. shen@nki.rfmh.org
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Rothman
authors.forename: Peter
authors.initials: P
authors.lastname: Brown
mesh_terms: D000328:Adult; D001923:Brain Chemistry; D003198:Computer Simulation; D003401:Creatine / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019047:Phantoms, Imaging; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D005680:gamma-Aminobutyric Acid; D005978:Glutathione; D003401:Creatine