title: Up-regulation of substance P and NMDA receptor mRNA in dorsal horn and preganglionic sympathetic neurons during adjuvant-induced noxious stimulation in rats.
abstract: Substance P (SP) and glutamate-containing terminals are found in the dorsal horn and preganglionic sympathetic neurons (PSNs) in the intermedio-lateral nucleus of the spinal cord. SP receptor (SPR) and N-methyl-D-aspartate type glutamate receptor (NMDAR) were also recognized in portions of the dorsal horn and PSNs. Primary sensory nerve fibers containing SP and glutamate terminated around PSNs, or partly on PSNs directly as well as on dorsal horn neurons (DHNs). The present study was performed to investigate the changes in SPR and NMDAR mRNA expressions during nociception in rats. Upon the injection of complete Freund's adjuvant (CFA) into the front paw, edema and hyperalgesia occurred immediately, with the difference in latency score between injected and non-injected paws continuing to day 10. The up-regulation of SPR and NMDAR mRNAs in DHNs and PSNs was recognized using in situ hybridization and northern blot techniques. CFA injection increased SPR mRNA expression in PSNs at days 1 and 4, and NMDAR mRNA expression at days 1, 4 and 7. At day 14, the mRNA expression of both receptors decreased to the control level. These changes in the amount of receptor mRNAs in DHNs and PSNs may cause hyperalgesia and sympathetically mediated pain.
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Chiba University, Japan. s.c.oh@pref-hosp.togane.chiba.jp
authors.forename: Seiji
authors.initials: S
authors.lastname: Ohtori
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Takahashi
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Ino
authors.forename: Tanemichi
authors.initials: T
authors.lastname: Chiba
authors.forename: Masatsune
authors.initials: M
authors.lastname: Yamagata
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sameda
authors.forename: Hideshige
authors.initials: H
authors.lastname: Moriya
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000503:physiopathology; D017403:In Situ Hybridization; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D010146:Pain / Q000235:genetics / Q000503:physiopathology*; D020671:Posterior Horn Cells / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D018040:Receptors, Neurokinin-1 / Q000235:genetics*; D013564:Sympathetic Nervous System / Q000502:physiology* / Q000503:physiopathology; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; D018040:Receptors, Neurokinin-1