Thyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis.
Remyelination in the adult central nervous system has been demonstrated in different experimental models of demyelinating diseases. However, there is no clear evidence that remyelination occurs in multiple sclerosis, the most diffuse demyelinating disease. In this article, we explore the possibility of promoting myelination in experimental allergic encephalomyelitis, a widely used experimental model of multiple sclerosis, by recruiting progenitors and channeling them into oligodendroglial lineage through administration of thyroid hormone (T4). A large number of proliferating cells (BrdUrd uptake and Ki67-IR) and the expression of markers for undifferentiated precursors (nestin) increased in the subventricular zone and spinal cord of experimental allergic encephalomyelitis animals. T4 administration reduces proliferation and nestin-immunoreactivity and up-regulates expression of markers for oligodendrocyte progenitors [polysialylated-neural cell adhesion molecule (PSA-NCAM), O4, A2B5] and mature oligodendrocytes (myelin basic protein) in the spinal cord, olfactory bulb, and subventricular zone.
Calza
Laura
L
Department of Veterinary Morphophysiology and Animal Production, University of Bologna, 40064 Ozzano Emilia, Bologna, Italy. lcalza@vet.unibo.it
Fernandez
Mercedes
M
Giuliani
Alessandro
A
Aloe
Luigi
L
Giardino
Luciana
L
D000818:Animals; D015415:Biomarkers; D002455:Cell Division; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005732:Gangliosides / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics; D012794:Sialic Acids / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013974:Thyroxine / Q000008:administration & dosage / Q000378:metabolism*
D015415:Biomarkers; D005732:Gangliosides; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D012794:Sialic Acids; C062875:ganglioside A2B5; C103469:polysialyl neural cell adhesion molecule; D020932:Nerve Growth Factor; D020796:Receptor, Platelet-Derived Growth Factor alpha; D013974:Thyroxine