title: Visualization of functionally activated circuitry in the brain.
abstract: We have used a transgenic approach to visualize functionally activated neurons and their projections. The transgenic mice contain a tau-lacZ fusion gene regulated by the promoter for c-fos, an immediate early gene that is rapidly induced in neurons after functional stimulation. Constitutive expression of beta-galactosidase (beta-gal), the lacZ product, was low and in accord with previous reports of c-fos expression. However, expression of beta-gal in positive neurons was clearly in cell bodies, axons, and dendrites. Treatment of the mice with kainic acid, a strong inducer of c-fos expression, resulted in high induction of beta-gal. beta-gal was induced in the same defined populations of neurons in the brain as those that express c-fos after kainic acid induction. Furthermore, the pattern of beta-gal expression within the neurons changed over time after kainic acid treatment. Early after kainate treatment, beta-gal was found mainly in cell bodies; at later times, expression extended further along the neuronal processes. This expression pattern is consistent with induction and anterograde transport of the Fos-Tau-beta-gal protein in the neurons. To test whether a functionally activated pathway could be visualized, transgenic mice were deprived of water, which activates nuclei involved in body fluid homeostasis. beta-gal induction was traced in neurons and their processes in the lamina terminalis, in magnocellular neurons of the supraoptic and paraventricular nuclei, and in their projections to the posterior pituitary gland. This strategy allowed the mapping of an activated osmoregulatory pathway. This transgenic approach may have general application in the mapping of functionally activated circuitry in the brain.
authors.affiliation: Department of Anatomy and Cell Biology and Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, 3010, Australia.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Wilson
authors.forename: Nupur
authors.initials: N
authors.lastname: Nag
authors.forename: Pamela
authors.initials: P
authors.lastname: Davern
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Oldfield
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McKinley
authors.forename: Ursula
authors.initials: U
authors.lastname: Greferath
authors.forename: Mark
authors.initials: M
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001931:Brain Mapping; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage; D015870:Gene Expression; D017930:Genes, Reporter; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D007608:Kainic Acid / Q000008:administration & dosage; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016716:PC12 Cells; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014867:Water; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018690:Excitatory Amino Acid Agonists; D016760:Proto-Oncogene Proteins c-fos; D016875:tau Proteins; D014867:Water; D001616:beta-Galactosidase; D007608:Kainic Acid