Imaging of the fluorescence spectrum of a single fluorescent molecule by prism-based spectroscopy.
We have devised a novel method to visualize the fluorescence spectrum of a single fluorescent molecule using prism-based spectroscopy. Equipping a total internal reflection microscope with a newly designed wedge prism, we obtained a spectral image of a single rhodamine red molecule attached to an essential light chain of myosin. We also obtained a spectral image of single-pair fluorescence resonance energy transfer between rhodamine red and Cy5 in a double-labeled myosin motor domain. This method could become a useful tool to investigate the dynamic processes of biomolecules at the single-molecule level.
Suzuki
Yoshikazu
Y
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro, 153-8902, Tokyo, Japan.
Tani
Tomomi
T
Sutoh
Kazuo
K
Kamimura
Shinji
S
D002232:Carbocyanines / Q000737:chemistry; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D020409:Molecular Motor Proteins / Q000737:chemistry; D018995:Myosin Heavy Chains / Q000737:chemistry; D015879:Myosin Subfragments / Q000737:chemistry; D024462:Myosin Type II / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
D002232:Carbocyanines; D005456:Fluorescent Dyes; D020409:Molecular Motor Proteins; D015879:Myosin Subfragments; D012235:Rhodamines; C085321:cyanine dye 5; D024462:Myosin Type II; D018995:Myosin Heavy Chains