Isomeric separation of methamphetamine by HPLC chiral column.
Methamphetamine and its active metabolite, amphetamine, are optically active compounds which, based upon synthetic routes, can be found in two forms; pure d-form and racemic mixture. Analysis of their isomers can help to identify which precursor is currently spreading widely in a given region. Since there are many drugs that can be metabolized to amphetamine/methamphetamine, isomeric separation can be a useful tool for evaluation of these drugs, as well. Indirect method by using N-trifluoroacetyl-1-prolyl chloride (1-TPC) was found to have limited accuracy due to the contribution effect. In this presentation a direct method using HPLC Chirex chiral column 3022 was studied. Although the method gave no base-line separation of two different isomer peaks, it gave good sensitivity, reliability, and linearity. No contribution effect was found in the method presented. It also gave excellent correlation with the 1-TPC method.
Lekskulchai
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Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007536:Isomerism; D008694:Methamphetamine / Q000302:isolation & purification* / Q000652:urine
D008694:Methamphetamine