NGF modulates CGRP synthesis in human B-lymphocytes: a possible anti-inflammatory action of NGF?
We investigated whether the sensory neuropeptide, calcitonin gene-related peptide (CGRP), could be synthesised by human lymphocytes. Our results indicate that in activated B-cells, there is a strong expression of CGRP gene transcripts, which is almost absent in resting cells. Since B-cells autocrinally produce NGF, the neutralisation of endogenous NGF by anti-NGF antibodies resulted in a marked reduction in CGRP expression in both resting and activated B-cells. Thus, NGF appears to directly affect the synthesis of CGRP in B-cells as in sensory neurons. By regulating CGRP synthesis in lymphocytes and neuronal cells, NGF can influence the intensity and duration of the immune response.
Bracci-Laudiero
Luisa
L
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Aloe
Luigi
L
Buanne
Pasquale
P
Finn
Anja
A
Stenfors
Carina
C
Vigneti
Eliana
E
Theodorsson
Elvar
E
Lundeberg
Thomas
T
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000378:metabolism*; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D006801:Humans; D020932:Nerve Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis
D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D015740:Calcitonin Gene-Related Peptide