Pulmonary granulomas of guinea pigs induced by inhalation exposure of heat-treated BCG Pasteur, purified trehalose dimycolate and methyl ketomycolate.
This study was designed to determine the identity of granulomatogenic substances in Mycobacterium bovis BCG Pasteur. When heat-treated BCG Pasteur bacilli were introduced into the lungs of guinea-pigs by an inhalation exposure apparatus, pulmonary granulomas without necrosis developed. Furthermore, when four kinds of mycolates derived from M. tuberculosis Aoyama B strain were introduced into the lungs by the same method, only trehalose 6,6'-dimycolate (TDM) and methyl ketomycolate induced pulmonary granulomas without central necrosis. The pulmonary granulomas consisted of epithelioid macrophages and lymphocytes. When a mixture of TDM and anti-TDM antibody was introduced into the lungs, development of granulomatous lesions was reduced. These data indicate that TDM and methyl ketomycolate are potent granulomatogenic reagents.
Sugawara
I
I
Udagawa
T
T
Hua
S C
SC
Reza-Gholizadeh
M
M
Otomo
K
K
Saito
Y
Y
Department of Molecular Pathology, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, and *4th Department of Internal Medicine, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8603, Japan.
Yamada
H
H
D000280:Administration, Inhalation; D000818:Animals; D003311:Cord Factors / Q000633:toxicity*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006099:Granuloma / Q000209:etiology*; D006168:Guinea Pigs; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000209:etiology*; D009163:Mycobacterium bovis / Q000276:immunology / Q000472:pathogenicity*; D009171:Mycolic Acids / Q000633:toxicity*
D003311:Cord Factors; D004269:DNA, Bacterial; D009171:Mycolic Acids