title: "De novo" sequencing of peptides recovered from in-gel digested proteins by nanoelectrospray tandem mass spectrometry.
abstract: Proteins separated by one-dimensional or two-dimensional gel electrophoresis can be digested in-gel with trypsin and the recovered peptides can be sequenced de novo using triple quadrupole or hybrid quadrupole time-of-flight instruments equipped with a nanoelectrospray ion source. The peptide sequences determined provide useful information for identification of proteins by homology searching for cloning of the cognate genes by PCR based approaches.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. shevchenko@mpi-cbg.de
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Igor
authors.initials: I
authors.lastname: Chernushevic
authors.forename: Anna
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Matthias
authors.initials: M
authors.lastname: Wilm
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
mesh_terms: D000818:Animals; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010103:Oxygen Isotopes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
chemical_list: D010103:Oxygen Isotopes; D010446:Peptide Fragments; D014357:Trypsin