[Effect of mouse p53 minigene on lung cancer cells with different 172 structures regulated by tetracycline].
OBJECTIVE
To investigate the effect of mouse 172 wild-type p53 (Arg), pseudo-wild-mutant-type p53 (Arg-->Leu) and mutant-type p53 (Arg-->His) induced by absence of tetracycline on the growth of PG cell line.


METHODS
Three variant types of p53 minigene were sub-cloned by gene recombination into an expression vector which was controlled by tetracycline. Through LipofectAMINE, the vectors were transfected into p53 defective PG (248CGG-->CTT) cells, and the transfectants were screened in the selecting medium containing puromycin. Tumor suppressing effects were studied by MTT absorption, flow cytometry and Western blotting.


RESULTS
Wild-type p53 and pseudo-wild-mutant-type p53 could lead cells to decrease their growth rates, arrest cell cycle and transactivation of p21(WAF1). Mutant-type p53 was defective in tumor suppression.


CONCLUSIONS
Wild-type p53 and pseudo-wild-type p53 may inhibit cell growth and induce cell cycle arrest. Some p53 variants such as 172 Arg-->Leu can still retain the tumor suppression function of the wild-type.
Ma
Y
Y
Department of Pathology, Health Science Center, Peking University, Beijing 100083, China.
Wu
B
B
Xie
J
J
You
J
J
Liu
J
J
Cui
X
X
Wang
J
J
Hui
P
P
D019943:Amino Acid Substitution; D000818:Animals; D001120:Arginine / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D009154:Mutation; D013752:Tetracycline / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000502:physiology
D016159:Tumor Suppressor Protein p53; D001120:Arginine; D013752:Tetracycline