[A preliminary study of loss of heterozygosity on chromosome 1p in primary hepatocellular carcinoma].
OBJECTIVE
Ten (10) loci on chromosome 1p were analyzed to detect LOH in 38 cases of hepatocellular carcinomas (HCC) in order to locate the deletion area and the possible deleted tumor suppressor gene (TSG) in HCC.


METHODS
PCR based microsatellite instability analysis were used to detect LOH on chromosome 1p31 and 1p35-p36 in HCC.


RESULTS
LOH were detected on chromosome 1p in 84.2% of HCC. High frequency of LOH (> 40%) were occurred at locus D1S186 on 1p31, locus D1S482 on 1p35 and loci D1S243, D1S160, D1S165 and D1S170 on 1p36.


CONCLUSIONS
LOH occurred mostly on chromosome 1p31 and 1p35-p36 in HCC. There may be more than two TSGs which associated with development of HCC on chromosome 1p31 and 1p35-p36.
Shao
J
J
Department of Pathology, Cancer Center, Sun Yat-Set University of Medical Sciences, Guangzhou 510060.
Li
H
H
Liew
C T
CT
Wu
Q
Q
Liang
X
X
Hou
J
J
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged
D004273:DNA, Neoplasm