Integrin alpha3beta1 (CD 49c/29) is a cellular receptor for Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) entry into the target cells.
Human herpesvirus-8 (HHV-8) is implicated in the pathogenesis of Kaposi's sarcoma. HHV-8 envelope glycoprotein B possesses the RGD motif known to interact with integrin molecules, and HHV-8 infectivity was inhibited by RGD peptides, antibodies against RGD-dependent alpha3 and beta1 integrins, and by soluble alpha3beta1 integrin. Expression of human alpha3 integrin increased the infectivity of virus for Chinese hamster ovary cells. Anti-gB antibodies immunoprecipitated the virus-alpha3 and -beta1 complexes, and virus binding studies suggest a role for alpha3beta1 in HHV-8 entry. Further, HHV-8 infection induced the integrin-mediated activation of focal adhesion kinase (FAK). These findings implicate a role for alpha3beta1 integrin and the associated signaling pathways in HHV-8 entry into the target cells.
Akula
Shaw M
SM
Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, KS 66160, USA.
Pramod
Naranatt P
NP
Wang
Fu Zhang
FZ
Chandran
Bala
B
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D019288:Herpesvirus 8, Human / Q000502:physiology*; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000032:analysis / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology*
D039201:Integrin alpha3beta1; D016023:Integrins; D011991:Receptors, Virus