[Profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis of rats induced by N, N'dinitrosopiperazine (DNP)].
OBJECTIVE
To investigate the profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis (NPC) induced by N, N'dinitrosopiperazine (DNP) and examined histolofically.


METHODS
Nasopharyngeal carcinomas of rats were induced by DNP and examined histologically. PCR-ELISA and nested RT-PCR were used to assay telomerase and telomerase RNA expression at different stages in the nasopharyngeal tissues of rats.


RESULTS
During the carcinogenesis process, telomerase activity increased along with the formation of a nasopharyngeal carcinoma. Telomerase expression was positively related with nasopharyngeal carcinogenesis. Telomerase RNA expression was present and did not change during the NPC process. Expression of telomease RNA was earlier than telomerase activation. Telomerase activation and telomerase RNA expressin were also detected in the pre-cancerous nasopharyngeal lesions.


CONCLUSIONS
Telomerase activation may participate in the onset and progression of NPC, and is an early step in NPC.
Tang
F
F
Xiangya Hospital, Hunan Medical University, Changsha 410078, China. yacao98@public.cs.hn.cn
Jiang
H
H
Duan
Z
Z
Chen
B
B
Jing
Z
Z
Wu
S
S
D000818:Animals; D018450:Disease Progression; D005260:Female; D008297:Male; D009303:Nasopharyngeal Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D009602:Nitrosamines; D011230:Precancerous Conditions / Q000139:chemically induced / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
D009602:Nitrosamines; D012333:RNA, Messenger; D019098:Telomerase; C026156:N,N'-dinitrosopiperazine