title: The developmental expression of vasoactive intestinal peptide (VIP) in cholinergic sympathetic neurons depends on cytokines signaling through LIFRbeta-containing receptors.
abstract: Sympathetic ganglia are composed of noradrenergic and cholinergic neurons. Cholinergic sympathetic neurons are characterized by the expression of choline acetyl transferase (ChAT), vesicular acetylcholine transporter (VAChT) and the vasoactive intestinal peptide (VIP). To investigate the role of cytokine growth factor family members in the development of cholinergic sympathetic neurons, we interfered in vivo with the function of the subclass of cytokine receptors that contains LIFRbeta as essential receptor subunit. Expression of LIFRbeta antisense RNA interfered with LIFRbeta expression and strongly reduced the developmental induction of VIP expression. By contrast, ganglion size and the number of ChAT-positive cells were not reduced. These results demonstrate a physiological role of cytokines acting through LIFRbeta-containing receptors in the control of VIP expression in sympathetic neurons.
authors.affiliation: Max-Planck-Institut für Hirnforschung, Abteilung Neurochemie, Deutschordenstr. 46, 60528 Frankfurt / Main, Germany.
authors.forename: Chi Vinh
authors.initials: CV
authors.lastname: Duong
authors.forename: Markus
authors.initials: M
authors.lastname: Geissen
authors.forename: Hermann
authors.initials: H
authors.lastname: Rohrer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002642:Chick Embryo; D002794:Choline / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003001:Cloning, Molecular; D050822:Cytokine Receptor gp130; D016207:Cytokines / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism*; D053606:Receptors, OSM-LIF; D016415:Sequence Alignment; D015398:Signal Transduction; D013564:Sympathetic Nervous System / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
chemical_list: D015703:Antigens, CD; D002352:Carrier Proteins; D016207:Cytokines; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D050822:Cytokine Receptor gp130; D014660:Vasoactive Intestinal Peptide; D002795:Choline O-Acetyltransferase; D002794:Choline