title: Spatial distribution of the 26S proteasome in meristematic tissues and primordia of rice (Oryza sativa L.).
abstract: The 26S proteasome is known to play central roles in the growth of many eukaryotes. However, little is known regarding its distribution in higher plants. Here, we report the spatial distribution pattern of Rpn3 (a regulatory PA700 subunit) and C2 (a subunit of the 20S proteasome) in rice ( Oryza sativa L.) seedlings as determined by in situ hybridization. The transcripts were abundantly co-expressed in the apical and marginal meristems of shoots and roots. Interestingly, these transcripts also accumulated in the leaf and ligule primordia of the shoot apex. Our results suggest that the 26S proteasome is spatially distributed among various tissues and may be involved not only in cell division but also in organ formation in higher plants.
authors.affiliation: Department of Bioproduction Science, Faculty of Horticulture, Chiba University, Matsudo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yanagawa
authors.forename: Seisuke
authors.initials: S
authors.lastname: Kimura
authors.forename: Tomomasa
authors.initials: T
authors.lastname: Takase
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Masaki
authors.initials: M
authors.lastname: Umeda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Komamine
authors.forename: Keiji
authors.initials: K
authors.lastname: Tanaka
authors.forename: Junji
authors.initials: J
authors.lastname: Hashimoto
authors.forename: Takahide
authors.initials: T
authors.lastname: Sato
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D018797:Cell Cycle Proteins / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D018519:Meristem / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010447:Peptide Hydrolases / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex; D021122:Protein Subunits; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D021122:Protein Subunits; C403291:RPN3 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease