[Bioactivity and test grafting of acellular dermal matrix containing fibroblasts].
OBJECTIVE
To investigate the bioactivity of acellular dermal matrix with fibroblasts and its role as dermal skeleton.


METHODS
Human fibroblasts (HFs) were planted onto the surface of acellular dermal matrix (ADM) to form living dermal substitute. The IL -- 6, IL -- 8 and TGF contents in the supernatant of the culture of HF -- ADM were determined with ELISA method, and the secretion of hyaluronic acid and laminin from extracellular matrix was measured with RIA method. The speed of vascularization and the wound contracture rate were observed after the dermal substitute was grafted on the full skin loss wound of Balb/c-nu mice (nude mice).


RESULTS
HFs grew very well after being planted onto ADM so as to form a single layer of cellular membrane. Many kinds of cytokines and extra cellular matrix components were secreted. Compared with simple acellular dermal grafting, the vascularization was accelerated, and the wound contracture rate decreased, after the living dermal substitute being grafted on the wound.


CONCLUSIONS
The ADM seeded with HFs exhibited excellent bioactivity and might be an optimal dermal substitute.
Xiao
S
S
Department of Burn, The Second Military Medical University, Shanghai, 200433 P. R. China.
Xia
Z
Z
Yang
J
J
D000818:Animals; D001808:Blood Vessels / Q000254:growth & development; D020405:Dermis / Q000098:blood supply / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016038:Skin Transplantation / Q000379:methods*; D017768:Skin, Artificial; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1; D014945:Wound Healing
D015850:Interleukin-6; D016209:Interleukin-8; D007797:Laminin; C508960:TGFB1 protein, human; C508961:Tgfb1 protein, mouse; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D006820:Hyaluronic Acid