Mast cell dynamics and involvement in the development of peritoneal adhesions in the rat.
Mast cells have been implicated in the ethiopathology of post-operative peritoneal adhesions. However an evaluation of their role in this condition is missing. Adhesions were induced in rats using small intestinal scraping. These rats or rats injected ip with either Stem Cell Factor (SCF) or nedocromil sodium or compound 48/80 (day 0-20) were sacrificed for grading of peritoneal adhesions, for evaluating mast cells and inflammatory cells in adhesions and peritoneal lavage (histochemical staining) and for histamine content (peritoneal lavage, radioenzymatic assay) on days 1-21. Mast cell sonicate was added to intestinal fibroblast and their proliferation was assessed (cell counting). All the rats developed adhesions (day 1) and after 3 days the adhesion score remained constant. Early adhesions were avascular and made of fibrinous exudate containing many mast cells. Thereafter adhesions became denser, and the number of stainable mast cells decreased and then stabilized. On the first few days, inflammatory cells in the peritoneal lavage increased while mast cells and histamine content were significantly reduced indicating their activation. Injection of SCF for 1 week slightly increased peritoneal adhesion formation while nedocromil sodium reduced their development. Compound 48/80 had no significant influence. Addition of mast cell sonicate to normal intestine or to peritoneal adhesion fibroblasts resulted in a significant increase of fibroblast proliferation. In conclusion, mast cell presence correlated with the establishment of peritoneal adhesions, and their pharmacological modulation influenced adhesion formation. In vitro mast cell induced fibroplasia. Therefore, mast cells have a profibrogenic role in this model of peritoneal adhesions.
Xu
Xiang
X
Department of Pharmacology, School of Pharmacy, The Hebrew Universiry-Hadassah Medical School, Jerusalem, Israel.
Pappo
Orit
O
Garbuzenko
Ekaterina
E
Bischoff
Stephan C
SC
Rivkind
Abraham
A
Levi-Schaffer
Francesca
F
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D006632:Histamine / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D017835:Nedocromil / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D010532:Peritoneal Diseases / Q000209:etiology* / Q000517:prevention & control; D051381:Rats; D019089:Stem Cell Factor / Q000494:pharmacology; D000267:Tissue Adhesions / Q000209:etiology / Q000517:prevention & control; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D019089:Stem Cell Factor; D017835:Nedocromil; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine