title: [Dynamic expression of tenascin in rat liver during liver fibrogenesis induced by CCl(4)].
abstract: OBJECTIVE
  To study the expression of tenascin in normal and fibrotic rat liver.
  
  
  METHODS
  Liver fibrosis induced in rat with CCl(4) were divided into three stages: the stage of hepatic injury (4 weeks), early stage of hepatic fibrosis (8 weeks) and later stage of hepatic fibrosis (12 weeks). Tenascin expression in liver tissue was observed by immunohistochemical method and in situ hybridization using digoxigenin-labelled DNA probe.
  
  
  RESULTS
  In normal rat liver there was a weak staining for tenascin. In both liver injury stage and early stage of hepatic fibrosis, both mRNA signal and immunostaining for tenascin were significantly increased as compared to that in normal liver. In later stage of hepatic fibrosis, mRNA signal and immunostaining for tenascin were decreased compared with that in early stage of hepatic fibrosis. The cellular source of tenascin in liver mainly restricted in mesenchymal cells.
  
  
  CONCLUSIONS
  Tenascin is a component of the extracellular matrix of liver tissue. Plays a role in early matrix organization during liver fibrogenesis.
authors.affiliation: Department of Gastroenterology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
authors.forename: Dingkang
authors.initials: D
authors.lastname: Yao
authors.forename: Shi
authors.initials: S
authors.lastname: Li
authors.forename: Xiantao
authors.initials: X
authors.lastname: Kong
authors.forename: Tingjun
authors.initials: T
authors.lastname: Ye
authors.forename: Jiangao
authors.initials: J
authors.lastname: Fan
authors.forename: Lan
authors.initials: L
authors.lastname: Zhong
authors.forename: Guoliang
authors.initials: G
authors.lastname: Wang
authors.forename: Liyan
authors.initials: L
authors.lastname: Tian
authors.forename: Wensen
authors.initials: W
authors.lastname: Wu
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019063:Tenascin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D019063:Tenascin; D002251:Carbon Tetrachloride