Use of Protein-A column and porcine factor VIII.
Extracorporeal immunoadsorption of factor VIII (FVIII) antibodies using Sepharose matrix columns coupled with staphylococcal Protein-A was reported two decades ago. The efficiency of this technique for removing FVIII antibodies of the IgG subtypes was clearly demonstrated. The recent widespread use of a variety of apheresis techniques for the management of a multitude of haematological and oncological conditions has made this technology more accessible and affordable. For the treatment of patients with FVIII inhibitors, the use of porcine FVIII makes it possible to control haemostasis with a therapeutic product for which in vitro testing can help predict the in vivo efficacy. By lowering the level of FVIII inhibitors, immunoadsorption can make the use of pFVIII concentrate possible in situations otherwise untreatable with FVIII preparations. Moreover, lowering the level of FVIII inhibitors by immunoadsorption allows adequate haemostasis to be achieved with much lower doses of FVIII leading to significant saving. Our preliminary data suggest that immunoadsorption combined with the use of pFVIII should be considered early in the treatment plan for controlling haemostasis in patients with FVIII inhibitors.
Rivard
G E
GE
Hemophilia Program, Hôpital Ste-Justine, 3175 Côte-Sainte-Catherine, Montreal, Quebec, Canada H3T 1C5.
D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D005169:Factor VIII / Q000008:administration & dosage* / Q000276:immunology*; D005260:Female; D006467:Hemophilia A / Q000139:chemically induced / Q000209:etiology / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D007166:Immunosuppressive Agents / Q000009:adverse effects; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000276:immunology; D012685:Sepharose / Q000031:analogs & derivatives* / Q000627:therapeutic use*; D013552:Swine; D013205:Staphylococcal Protein A
D001323:Autoantibodies; D005169:Factor VIII; D007166:Immunosuppressive Agents; D007518:Isoantibodies; D012685:Sepharose; C042030:Staphylococcal protein A-sepharose; D013205:Staphylococcal Protein A