Herpes simplex virus type 1-mediated transfer of neurotrophin-3 stimulates survival of chicken auditory sensory neurons.
Neurotrophin-3 (NT-3) is one of the most potent stimulators for survival of auditory sensory neurons. Viral transfer of neurotrophins into auditory neurons may offer a route to provide a permanent supply of the growth factor and guarantee their long-term survival. Herpes simplex virus type 1 (HSV-1)-based vectors have demonstrated their effectiveness to transfer genes into peripheral sensory neurons. In the present report, we have produced a HSV-1-based amplicon vector expressing NT-3. This vector efficiently infects isolated auditory neurons and stimulates their survival during distinct developmental stages of the inner ear. Therefore, this vector may present a unique entry point to develop therapies preventing or treating hearing impairment caused by the degeneration of auditory neurons.
Carnicero
Estela
E
Instituto de Biología y Genética Molecular, Universidad de Valladolid y Consejo Superior de Investigaciones Cientificas, Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Medicina, 47005 Valladolid, Spain.
Knipper
Marlies
M
Tan
Justin
J
Alonso
Maria Teresa
MT
Schimmang
Thomas
T
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics*; D002478:Cells, Cultured; D002642:Chick Embryo; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics / Q000627:therapeutic use*; D006319:Hearing Loss, Sensorineural / Q000503:physiopathology / Q000517:prevention & control / Q000628:therapy; D018259:Herpesvirus 1, Human / Q000235:genetics*; D009410:Nerve Degeneration / Q000503:physiopathology / Q000517:prevention & control / Q000628:therapy; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D013136:Spiral Ganglion / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D014404:Tubulin / Q000378:metabolism
D020933:Neurotrophin 3; D014404:Tubulin