Effects of Misoprostol with Interleukin-1 on Proteoglycan Metabolism of Cultured Articular Chondrocytes.
Bovine and normal human articular chondrocytes in suspension culture were treated with misoprostol (an analog of prostaglandin E(1) [PGE(1)]), alone and in combination with interleukin-1 (IL-1) and/or diclofenac sodium, to study effects on proteoglycan metabolism. A concentrations of 50 ng ml(minus sign1) and above, misoprostol suppressed synthesis of proteoglycans but did not affect their rate of catabolism. The mild inhibitory effect of misoprostol on proteoglycan synthesis was additive to that of IL-1, especially in chondrocytes from the superficial zone of bovine articular cartilage. In IL-1-treated cultures, diclofenac NA caused a modest improvement in proteoglycan synthesis, but this beneficial effect was diminished by the simultaneous addition of misoprostol. Cartilage or chondrocyte cultures treated with IL-1, in which proteoglycan synthesis is suppressed, serve as model systems in which to study metabolic responses of chondrocytes to potential therapeutic agents, but in these experiments, no chondroprotective effects of misoprostol were observed in IL-1-activated chondrocytes.
Aydelotte
Margaret B.
MB
Department of Biochemistry, Rush Medical College at Rush--Presbyterian--St. Luke's Medical Center, Chicago, USA.
Mok
Su S.
SS
Michal
Lincoln
L
Häuselmann
Hans J.
HJ