Detection and typing of respiratory adenoviruses in a single-tube multiplex polymerase chain reaction.
A multiplex polymerase chain reaction (PCR) assay that is capable of detecting and typing six serotypes of respiratory adenovirus (Ad) was developed, using multiple sets of type-specific primers. The detection of each different serotype depended on distinguishing different numbers and sizes of amplification products on agarose gels following PCR. The multiplex PCR was tested with 26 clinical Ad isolates and other respiratory viruses including influenza viruses, parainfluenza viruses, and respiratory syncytial viruses as well as respiratory bacterial pathogens such as Chlamydia pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, and Mycoplasma pneumoniae. The multiplex PCR for the detection and typing of Ads gave an excellent correlation with the results by conventional typing with type-specific antisera. This assay may serve as a rapid means of confirming Ad with simultaneous serotype identification of the isolates. It will also have relevance as an adjunctive tool to conventional serotyping for diagnostic and epidemiological purposes.
Na
Byoung-Kuk
BK
Laboratory of Respiratory Viruses, Department of Virology, National Institute of Health, Seoul, Korea.
Kim
Jee-Hee
JH
Shin
Gu-Choul
GC
Lee
Joo-Yeon
JY
Lee
Jin-Soo
JS
Kang
Chun
C
Kim
Woo-Joo
WJ
D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012141:Respiratory Tract Infections / Q000821:virology*; D012680:Sensitivity and Specificity
D004279:DNA, Viral