A haplolethal locus uncovered by deletions in the mouse T complex.
Proper levels of gene expression are important for normal mammalian development. Typically, altered gene dosage caused by karyotypic abnormalities results in embryonic lethality or birth defects. Segmental aneuploidy can be compatible with life but often results in contiguous gene syndromes. The ability to manipulate the mouse genome allows the systematic exploration of regions that are affected by alterations in gene dosage. To explore the effects of segmental haploidy in the mouse t complex on chromosome 17, radiation-induced deletion complexes centered at the Sod2 and D17Leh94 loci were generated in embryonic stem (ES) cells. A small interval was identified that, when hemizygous, caused specific embryonic lethal phenotypes (exencephaly and edema) in most fetuses. The penetrance of these phenotypes was background dependent. Additionally, evidence for parent-of-origin effects was observed. This genetic approach should be useful for identifying genes that are imprinted or whose dosage is critical for normal embryonic development.
Browning
Victoria L
VL
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Bergstrom
Rebecca A
RA
Daigle
Sandra
S
Schimenti
John C
JC
D000818:Animals; D005260:Female; D017353:Gene Deletion; D018628:Gene Dosage; D006239:Haplotypes; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins / Q000235:genetics*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region; D000099156:Superoxide Dismutase 2
D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D013482:Superoxide Dismutase; D044767:Ubiquitin-Protein Ligases; D000099156:Superoxide Dismutase 2; C116196:PPP1R11 protein, human