Analysis of genetic aberrations in uterine adenomyosis using comparative genomic hybridization.
OBJECTIVE
To use comparative genomic hybridization (CGH) to analyzefrozen tissuesfrom adenomyosis cases to discover and map genomic regions for chromosomal gains and losses.


METHODS
In a retrospective study, upper and lower threshold values of 1.20 and 0.80, respectively, were used to define positive findings.


RESULTS
No positive recurrent gene copy number alterations were detected in the 25 cases of pathologically proven adenomyosis.


CONCLUSIONS
Although CGH is extremely useful in investigating candidate genes in the development of adenomyosis, CGH was not useful in this study. Genetic changes might be indeed extremely rare in adenomyosis, or CGH was not sensitive enough to detect candidate genes.
Wang
Peng-Hui
PH
Department of Obstetrics and Gynecology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan. phwang@vghtpe.gov.tw
Shyong
Wen-Yuann
WY
Lin
Chi-Hung
CH
Chen
Yann-Jang
YJ
Li
Ywan Feng
YF
Chao
Hsiang-Tai
HT
Yuan
Chiou-Chung
CC
D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis*; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D005260:Female; D018628:Gene Dosage; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D012189:Retrospective Studies; D014591:Uterine Diseases / Q000235:genetics* / Q000473:pathology / Q000601:surgery
D004247:DNA