[A study of lipid peroxide-induced damage and of ultrastructure of lens epithelial cells in lens organ culture in vitro].
OBJECTIVE
To understand the mechanism of the rabbit lens damage from lipid peroxidation (LPO) involved in cataract development.


METHODS
Ultraviolet (UV) radiation was used to induce the damage of peroxidation on cultured rabbit lens. Cultured rabbit lenses were exposed to UV radiation in MEM cultural medium without or with superoxide dismutase (SOD) added at 10 U/ml. The thiobarbituric acid (TBA) colorimetric method was used to detect the lens malondialdehyde (MDA) content produced by LPO, and electron microscopy was used to observe the ultrastructure of rabbit lens epithelium.


RESULTS
In the lens with UV radiation, MDA was increased as compared with that in non-irradiated one (P < 0.01) and in UV + SOD group (P < 0.01). The membranes of lens epithelial cells were damaged and mitochondria, etc. membranous structures disappeared with clumping pattern of chromatin, while the cells in UV + SOD and control groups appeared normal in structure.


CONCLUSIONS
UV radiation can induce lipid peroxide damage in lens culture of rabbit.
Kang
J
J
The PLA Dalian Junior College of Medicine, Dalian 116013.
Zhang
J
J
Zhao
Y
Y
D000818:Animals; D004847:Epithelial Cells / Q000528:radiation effects; D005260:Female; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D008054:Lipid Peroxides / Q000633:toxicity*; D008297:Male; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D011817:Rabbits; D014466:Ultraviolet Rays
D008054:Lipid Peroxides