[V-ATPase inhibitor baflomycine A1 inhibits bone resorption by osteoclast-like cells].
OBJECTIVE
In order to compare the role of osteoclast-like cells (OLCs) originating from giant cell tumor of bone and the osteoclasts (OCs) on bone resorption, as well as to study which kind of proton pump is associated with bone resorption by the OLCs and its role related.


METHODS
Culture of OLCs together with the bone slices, the number of pits and the surface areas formed by OLCs was counted. The effects of F0F1-ATPase inhibitor, oligmycine V-ATPase inhibitor, baflomycine of OLCs on bone resorption were compared.


RESULTS
Baflomycine are considered effective in inhibiting bone resorption but no definited effect obtained with oligmycine.


CONCLUSIONS
OLCs bear the similar bone resorption mechanism as that of OCs. It's considered that OLCs and OCs are derived from the same origin.
Zhao
N
N
Department of Pathology, School of Stomatology, Beijing Medical University, Beijing 100081.
Yu
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001862:Bone Resorption / Q000517:prevention & control*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005870:Giant Cell Tumors / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D018942:Macrolides; D008854:Microscopy, Electron; D009840:Oligomycins / Q000494:pharmacology; D010010:Osteoclasts / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D025262:Vacuolar Proton-Translocating ATPases / Q000037:antagonists & inhibitors*
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D018942:Macrolides; D009840:Oligomycins; C040929:bafilomycin A1; D025262:Vacuolar Proton-Translocating ATPases