Approaches toward the directed design of a vaccine against Borrelia burgdorferi.
The overall efficacy of a recombinant vaccine for Lyme disease that is effective worldwide will depend upon the selection of one or more immunoprotective target(s) and the frequency of genetic variation, which can alter the antigenicity of the immunoprotective epitopes of the target proteins. Careful delineation of these protective epitopes on target antigens is essential for the development of vaccine candidates as well as for understanding the limitations of such vaccines. Structural models of these targets will provide critical information about conformation and specific residue surface accessibility for defining protective epitopes. Co-crystal structures with Fab fragments of protective antibodies will further delineate critical antigen surfaces. Population genetics will provide vital information on the heterogeneity of these proteins. Detailed epitope mapping will provide the information needed for the bioengineering of antigens needed to expand the specificity of a candidate vaccine.
Luft
Benjamin J
BJ
Department of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-8160, USA. bluft@mail.som.sunysb.edu
Dunn
John J
JJ
Lawson
Catherine L
CL
D000954:Antigens, Surface / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines; D025065:Borrelia burgdorferi / Q000276:immunology*; D015195:Drug Design; D008074:Lipoproteins; D008193:Lyme Disease / Q000517:prevention & control*; D022123:Lyme Disease Vaccines / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008958:Models, Molecular; D011487:Protein Conformation
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D022123:Lyme Disease Vaccines; C072045:OspA protein