[Effects of Asiaticoside on hypertrophic scars in a nude mice model].
OBJECTIVE
To study the effect of Asiaticoside on the fibroblast collagen biosynthesis in vitro culture and on the hypertrophic scar in a nude mice model, and its mechanism.


METHODS
Light microscope (LM) and electron microscope (EM) were employed to study the morphological changes in fibroblast before and after the application of Asiaticoside. The fibroblast collagen synthesis was assayed by (3)H-proline incorporation. A nude mice model with hypertrophic scar was established for observing the effect of Asiaticoside given by local injection.


RESULTS
It was found that Asiaticoside could significantly affect the ultrastructure of fibroblast and inhibit its collagen synthesis in vitro culture (P < 0.01) in a manner of dose-effect relationship. Local injection of Asiaticoside into the nude mouse body could inhibit the proliferation of scar without any toxic effect.


CONCLUSIONS
The possible mechanism of the effect of Asiaticoside on hypertrophic scars is related to its inhibitory action on the fibroblast proliferation and collagen synthesis.
Qi
S
S
Department of Burn, First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China.
Xie
J
J
Li
T
T
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy* / Q000473:pathology; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014315:Triterpenes / Q000627:therapeutic use*
D014315:Triterpenes; D003094:Collagen; C004446:asiaticoside