title: Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.
abstract: Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats.
authors.affiliation: Department of Genetics, Lund University, Sweden.
authors.forename: Monika
authors.initials: M
authors.lastname: Rosén
authors.forename: Jan-Erik
authors.initials: JE
authors.lastname: Edström
mesh_terms: D000818:Animals; D001483:Base Sequence; D002683:Chironomidae / Q000235:genetics*; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics*; D005090:Exodeoxyribonucleases; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides; D016615:Telomere / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III