title: [The effects of epinephrine and adrenergic antagonists on adenosine 3', 5'-monophosphate level of bovine trabecular cells in vitro].
abstract: OBJECTIVE
  To determine the effects of epinephrine (EPI) and adrenergic antagonists on adenosine 3', 5'-monophosphate (cAMP) level of bovine trabecular cells (BTC) in vitro.
  
  
  METHODS
  (3)H-cAMP was used in protein binding assay for measuring the intracellular level of cAMP.
  
  
  RESULTS
  (1) 10(-5) mol/L EPI induced a fold increase of cAMP in cultured BTC in vitro; (2) Timilol and ICI 118, 551 blocked efficiently the effect of EPI at a lower concentration (10(-6) mol/L). (3) Bisoprolol did not efficiently block the effect of EPI unless at high concentrations (>or= 10(-5) mol/L).
  
  
  CONCLUSIONS
  The effects of EPI increasing outflow facility may be associated with its increase of cAMP in trabecular cells; BTC contains beta-adrenergic receptors, and beta(2)-adrenergic receptors are dominant.
authors.affiliation: Department of Ophthalmology, The First Clinical Hospital, Beijing Medical University, Beijing 100034.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Shen
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D007429:Intraocular Pressure / Q000187:drug effects; D014129:Trabecular Meshwork / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*
chemical_list: D000319:Adrenergic beta-Antagonists; D000242:Cyclic AMP; D004837:Epinephrine