title: Analysis of the human herpesvirus 8 (HHV-8) genome and HHV-8 vIL-6 expression in archival cases of castleman disease at low risk for HIV infection.
abstract: Lymph nodes from 44 patients with Castleman disease (CD) without risk factors for HIV infection were analyzed with polymerase chain reaction (PCR), in situ hybridization (ISH), and immunohistochemical analysis for human herpesvirus 8 (HHV-8) and viral interleukin-6 (vIL-6). PCR detected HHV-8 genome in 2 of 4 cases; ISH detected it in 9 of 16 cases. HHV-8 vIL-6 peptides were detected in 2 of 44 cases. vIL-6- and ISH-positive cells were found in large transformed and small lymphocytes of the follicular mantle, respectively. Of 9 cases of plasma cell (PC) CD that demonstrated HHV-8 genome by PCR or ISH, 1 expressed vIL-6. Clonal populations of PCs in CD by immunohistochemical analysis or immunoelectrophoresis of serum and urine were associated with neuropathy. HHV-8 vIL-6 detection was associated with poor survival and lack of HHV-8 IL-6, with low risk for subsequent lymphoma. Although HHV-8 genome was detected in a considerable number of patients with PC CD, vIL-6 expression was infrequent. Expression of HHV-8 vIL-6 in CD may indicate poor prognosis in patients at risk for lymphoma who may prospectively require more aggressive treatment. The lack of vIL-6 expression in CD with HHV-8 genome suggests that human IL-6 rather than vIL-6 may be the principal pathogenic cytokine.
authors.affiliation: Department of Pathology, Mayo Clinic, Jacksonville, FL 32224, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Menke
authors.forename: Amy
authors.initials: A
authors.lastname: Chadbum
authors.forename: Ethel
authors.initials: E
authors.lastname: Cesarman
authors.forename: Eric
authors.initials: E
authors.lastname: Green
authors.forename: James
authors.initials: J
authors.lastname: Berenson
authors.forename: Jonathan
authors.initials: J
authors.lastname: Said
authors.forename: Markus
authors.initials: M
authors.lastname: Tiemann
authors.forename: Reza
authors.initials: R
authors.lastname: Parwaresch
authors.forename: Stephan D
authors.initials: SD
authors.lastname: Thome
mesh_terms: D005871:Castleman Disease / Q000453:epidemiology / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D015897:Comorbidity; D018450:Disease Progression; D015658:HIV Infections / Q000453:epidemiology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D010950:Plasma Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D018570:Risk Assessment; D012307:Risk Factors; D012680:Sensitivity and Specificity; D015996:Survival Rate; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015850:Interleukin-6; D014764:Viral Proteins