[The lipase/lipoxygenase bienzyme system in AOT reversed micelles in octane].
In this work it is shown that the bienzyme lipase/lipoxygenase system can function in reversed micelles of bis(2-ethyl)hexyl sulfosuccinate (AOT) in octane. As a lipase substrate, a fish fat preparation (fat of sea mammals) with a high content of polyunsaturated fatty acids was used. It was demonstrated that the bienzyme reaction proceeded in a stationary mode and had a rate-limiting step catalyzed by lipase. Under optimal conditions, the efficacy of functioning of the bienzyme system was by an order of magnitude higher than that in water. The lipase/lipoxygenase bienzyme system can be used as a new method of spectrophotometric determination of lipase activity. The English version of the paper.
Pavlenko
I M
IM
Moscow State University, Chemical Faculty, Vorob'evy gory, GSP-3 Moscow, 119899 Russia.
Kuptsova
O S
OS
Kliachko
N L
NL
Levashov
A V
AV
D000818:Animals; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry*; D005395:Fish Oils / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008084:Lipoxygenase / Q000737:chemistry* / Q000378:metabolism; D008823:Micelles; D009795:Octanes / Q000737:chemistry*
D005395:Fish Oils; D008823:Micelles; D009795:Octanes; D004143:Dioctyl Sulfosuccinic Acid; D008084:Lipoxygenase; D008049:Lipase; C026728:octane