Expression of TrkB receptors in developing visual cortex is not regulated by light.
1. Neurotrophins are very good candidates which relate electrical activity to molecular changes in activity-dependent phenomena. They exert their action through binding to specific tyrosine-kinase receptors: Trk receptors. It is important to consider Trk distribution in order to understand better the role of neurotrophins in the Central Nervous System (CNS). We focused our attention on brain-derived neurotrophic factor (BDNF) Trk receptors (TrkB) during development of the rat visual cortex, since this neurotrophin has been shown to play an important role in visual system development and plasticity. 2. We investigated the full length form of TrkB receptors considering both its total amount and its cellular distribution. To address this issue we used an antibody that recognizes the full length form of TrkB and we used it both in Western blot and immunohistochemistry. 3. We found that the expression of TrkB receptor increases during development, but that there is no effect on visual experience, since dark-reared animals show the same protein level and pattern of TrkB expression compared to age-matched, normally reared controls.
Tropea
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D
Scuola Internazionale Supériore di Studi Avanzati, Trieste, Italy.
Domenici
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L
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000528:radiation effects; D003624:Darkness; D018507:Gene Expression Regulation, Developmental / Q000502:physiology* / Q000528:radiation effects; D066298:In Vitro Techniques; D008027:Light; D051379:Mice; D051381:Rats; D020813:Receptor, trkB / Q000235:genetics* / Q000528:radiation effects; D014793:Visual Cortex / Q000254:growth & development / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D020813:Receptor, trkB