Spectrophotometric methods for the rapid determination of menadione and menadione sodium bisulphite and their application in pharmaceutical preparations.
Two simple, rapid and sensitive spectrophotometric determination of menadione and its sodium bisulphite derivative (MSB) have been carried out. The first method involves the reaction of menadione and its sodium bisulphite derivative with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) is sodium hydroxide medium to give blue coloured product having maximum absorption at 625 nm and the coloured species is stable for more than 1 h. The Beer's law is obeyed in the range 0.4-16 microg ml(-1). The second method proposes the reaction of menadione and its sodium bisulphite derivative with resorcinol in concentrated sulphuric acid medium to give red coloured product having maximum absorption at 520 nm and is stable for 3 h. The Beer's law was obeyed in the range of 1-24 microg ml(-1). Molar absorptivity for the above two methods were found to be 7.6 x 10(3) and 4.5 x 10(3) l mol(-1) cm(-1), respectively. All the measurements were carried out at room temperature. These two methods have been successfully applied for menadione and its sodium bisulphite derivatives in injections and tablets of pharmaceutical formulations. The results compare favourably with official method.
Nagaraja
P
P
Department of studies in Chemistry, Mysore University, Manasa Gangotri, India. nagaraja@mailcity.com
Vasantha
R A
RA
Yathirajan
H S
HS
D000933:Antifibrinolytic Agents / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D004355:Drug Stability; D016014:Linear Models; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods / Q000706:statistics & numerical data; D013696:Temperature; D024483:Vitamin K 3 / Q000032:analysis* / Q000737:chemistry
D000933:Antifibrinolytic Agents; D004364:Pharmaceutical Preparations; D024483:Vitamin K 3