Comparative study of the purification and characterization of the cytosolic glutathione S-transferases from two salmonid species: Atlantic salmon (Salmo salar) and brown trout (Salmo trutta).
In the present report, an efficient method for isolating multiple cytosolic forms of glutathione S-transferases from liver and kidney cytosolic samples of two salmonid species (brown trout and Atlantic salmon) is described, and some of the multiple properties of these enzymes are presented. Glutathione S-transferases were partially purified by low-pressure affinity chromatography on a column with glutathione coupled to agarose, which retained an average of 89.47% of the total activity. The GST activity was appropriated towards CDNB and ETHA as substrates. The application of an HPLC system associated to elestrospray ionization mass spectrometry allowed the identification of five GST cytosolic isoforms, corresponding to subunits with M(r) between 23,700 and 26,900 Da being the main form, with retention time of 17 min, a pi-class-related GST isoenzyme.
Nóvoa-Valiñas
M C
MC
Department of Toxicology, Facultad de Veterinaria, Avda. de Madrid s/n. 27002 Lugo, Spain.
Pérez-López
M
M
Melgar
M J
MJ
D000818:Animals; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D005982:Glutathione Transferase / Q000737:chemistry* / Q000302:isolation & purification; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D012474:Salmon / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D014337:Trout / Q000378:metabolism*
D007527:Isoenzymes; D005982:Glutathione Transferase