[Effect of serum and wound exudates on PMN apoptosis in burned patients].
OBJECTIVE
To study in vitro the effect of burn serum and wound exudates on PMN apoptosis in burned patients.


METHODS
The burn serum was obtained from patients within 3 days postburn. The blister fluid was collected from intact blisters in burn wound within 24 hours postburn, and subeschar tissue fluid underneath III degree burn wound during escharectomy. PMNs were isolated from healthy human donors and cultured for 24 hours at 37 degreeC and 5% CO(2) with normal serum (NS), burn serum (BS), burn blister fluid (BBF) and subeschar tissue fluid (STF), respectively. Morphological assessment and quantification of PMN apoptosis were performed with AO + EB stain and flow cytometric analysis.


RESULTS
The number of apoptotic PMN and DNA fragmentation of PMN significantly decreased (P < 0.05) when PMN were cultured with BS, BBF, and STF when compared with that with NS. In addition, the specific DNA "ladder" pattern for apoptotic cells couldn't be found on the agarose gel electrophoresis in this case. However, BBF exhibited weaker inhibiting effect than BS and STF on PMN apoptosis.


CONCLUSIONS
PMN apoptosis could be inhibited in vitro by BS, STF and BBF from burned patients.
Zhang
P
P
Department of Burns and Plastic Surgery, XiangYa Hospital, Hunan Medical University, Changsha 410078, P.R. China.
Ma
E
E
Huang
X
X
D017209:Apoptosis; D002056:Burns / Q000097:blood*; D053938:DNA Fragmentation; D005122:Exudates and Transudates / Q000502:physiology*; D006801:Humans; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000502:physiology*