[Effects of thrombin peptides on wound healing and proliferation and migration of normal human epidermal keratinocyte (NHEK)].
OBJECTIVE
To define the effects of thrombin peptides on wound healing and NHEK proliferation and migration.


METHODS
A wound model was made with four 1.5 cm circular full thickness dermal excisions on the back of each Sprague-Dawley rat. 0.1 microgram (40 microliter) TP508 was applied to each circular excisional wound in 9 rats, the other 9 received saline only. Wound area was calculated with JAVA Jandel and IMAGE PRO software. NHEK945 proliferation was assessed by MTT assay and direct cell count with a Coulter Counter. Cell migration was determined by 48-well Boyden Chamber. Cells migrated onto the lower surface of the filter were assessed by a Chemi Imager 4000 Image Analyzer and expressed as spot density.


RESULTS
Wound area in rats treated with TP508 was 73.7% and 45.4% of saline control on day 7 and 14, respectively. NHEK945 proliferation was accelerated after adding thrombin and TP508. The spot density of migrated cells was 76.7 plus minus 13.8 in medium alone. After adding 1 microgram/ml of thrombin and 10 microgram/ml of TP508, the spot density was 104.4 plus minus 12.2 and 109.4 plus minus 14.6, respectively.


CONCLUSIONS
Results of this study suggest that both thrombin and TP508 have significant actions on wound healing and NHEK proliferation and migration, which is important in wound repair.
Huang
Y
Y
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038.
Yang
Z
Z
Carney
D
D
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013917:Thrombin / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D013917:Thrombin