title: Direct role of human dermal fibroblasts and indirect participation of epidermal keratinocytes in MMP-1 production after UV-B irradiation.
abstract: In vivo, matrix metalloproteinases are produced in response to ultraviolet B (UV-B) irradiation and are considered to be involved in connective tissue alterations observed in photoaging. The respective roles of keratinocytes and fibroblasts in UV-B-induced MMP-1 production were investigated in monolayer cultures of keratinocytes and fibroblasts as well as in an epidermis model reconstructed in vitro. In contrast to fibroblasts, which secreted MMP-1 in response to UV-B irradiation, no accumulation of MMP-1 was observed after UV-B irradiation of keratinocytes. However, culture medium from UV-B-irradiated keratinocytes, which showed an increase in IL-1alpha and IL-6, induced MMP-1 production by human fibroblasts, suggesting that UV-B irradiation modulates MMP-1 production via both direct and indirect mechanisms.
authors.affiliation: L'OREAL, Advanced Research Laboratories, Centre Charles Zviak, Clichy, France. dfagot@recherche.loreal.com
authors.forename: Dominique
authors.initials: D
authors.lastname: Fagot
authors.forename: Daniel
authors.initials: D
authors.lastname: Asselineau
authors.forename: Françoise
authors.initials: F
authors.lastname: Bernerd
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D051379:Mice; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D020781:Matrix Metalloproteinase 1