title: Focal cerebral ischemia in rats produced by intracarotid embolization with viscous silicone.
abstract: Many factors contribute to the severity of neuronal cell death and the functional outcome in stroke. We describe an embolic model of focal cerebral ischemia in the rat that does not require craniotomy and is compatible with continuous measurement of regional CBF using multichannel laser Doppler flow (LDF) technique. Either a 22 microliters (large lesion) or 11 microliters (small lesion) bolus of viscous silicone was injected cephalad into the internal carotid artery. Upon injection, LDF decreased abruptly, most severely in the parietal cortex (-74% +/- 5%) in the large lesion and in the occipital cortex (-69% +/- 10%) in the small lesion model. Over the first hour, post-embolization LDF improved in most areas (e.g. -48% +/- 9% parietal, large lesion) but declined in the small lesion group in the occipital region (-81% +/- 8%). CBF measured by [C]14-IAP autoradiography 1 h post-embolization in the large lesion model demonstrated near-hemispheric ischemia (70% of hemisphere) with sparing of cingulate cortex. Autoradiography demonstrated that ischemia in the small lesion was largely cortical. Light microscopy of brains embolized with 11 microliters of dyed silicone showed filling of pial vessels with no silicone in the Circle of Willis or parenchyma. No animals in the large lesion group survived 24 h. Thirteen of 15 animals in the small lesion group survived for two weeks with resolution of initial hemiplegia, ocular asymmetry and weight loss. Hematoxylin-eosin staining two weeks post-embolization showed signs of severe hypoxia and infarction. In conclusion, the intracarotid silicone embolization technique produces a titrable, reproducible permanent ischemic injury by blocking perfusion in the pial circulation, and is amenable to multisite monitoring with laser Doppler flowmetry. The smaller embolus produces cortical infarction with high rate of survival and neurological recovery.
authors.affiliation: Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA. klauer@mcw.edu
authors.forename: Kathryn K
authors.initials: KK
authors.lastname: Lauer
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.forename: Elliot A
authors.initials: EA
authors.lastname: Stein
authors.forename: Khang-cheng
authors.initials: KC
authors.lastname: Ho
authors.forename: John P
authors.initials: JP
authors.lastname: Kampine
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Hudetz
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D002545:Brain Ischemia / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D002339:Carotid Arteries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D002544:Cerebral Infarction / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000502:physiology; D002561:Cerebrovascular Disorders / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D004396:Coloring Agents; D004195:Disease Models, Animal; D018450:Disease Progression; D020766:Intracranial Embolism / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017655:Silicon Compounds / Q000737:chemistry; D020521:Stroke / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors; D014656:Vascular Surgical Procedures / Q000295:instrumentation* / Q000379:methods
chemical_list: D004396:Coloring Agents; D017655:Silicon Compounds