[Stimulation of peripheral delta1-opioid receptors as a method of preventing ischemic and reperfusion arrhythmia: role of K(ATP)-channels].
Preliminary administration of the delta 1-opioid receptor (delta 1-OR) peptide agonist DPDPE (0.5 mg/kg, i.v.) decreased the incidence of occlusion (10 min) and reperfusion (10 min) arrhythmias in rats. The delta 2-OR agonist DSLET did not affect arrhythmias upon the coronary artery occlusion and reperfusion. Pretreatment with the selective delta-antagonists ICI 174,864 (2.5 mg/kg) or TIPP[psi] (0.5 mg/kg) completely eliminated the antiarrhythmic effect of DPDPE. Uncapable of crossing the blood brain barrier, the nonselective OR antagonist naloxone methiodide (5 mg/kg) also abolished this effect. At the same time, hexametonium (10 mg/kg) did not antagonize the antiarrhythmic effect of DPDPE. Pretreatment with the KATP channel blocker glibenclamide (0.3 mg/kg) completely eliminated the protective effect of the delta 1-OR stimulation. It was concluded that the delta 1-OR stimulation prevents the ischemic and reperfusion arrhythmias by means of the KATP channel activation.
Maslov
L N
LN
Laboratory of Experimental Cardiology, Institute of Cardiology, Tomsk Scientific Center, Siberian Division, Russian Academy of Medical Sciences, ul. Shevchenko 24, Tomsk, 634050 Russia.
Krylatov
A V
AV
Lishmanov
A Iu
AIu
Podoksenov
Iu K
IuK
Solenkova
N V
NV
Bogomaz
S A
SA
Tam
S W
SW
Gross
G J
GJ
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000503:physiopathology / Q000517:prevention & control*; D020881:Enkephalin, D-Penicillamine (2,5)- / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D017465:Receptors, Opioid, delta / Q000819:agonists* / Q000037:antagonists & inhibitors; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*
D009842:Oligopeptides; D015221:Potassium Channels; D017465:Receptors, Opioid, delta; D009270:Naloxone; D020881:Enkephalin, D-Penicillamine (2,5)-; C092825:ICI 174865