[Comparison of serological and DNA typings for HLA-AB of cord blood samples].
OBJECTIVE
To evaluate the practical value of serological method for HLA-AB typing of cord blood samples.


METHODS
Serological typing results of 1800 cord blood samples were analyzed and 36 of them were compared with that of PCR-SSP (polymerase chain reaction-sequence specific primers). Lymphocytes in cord blood samples were isolated by T-immunomegnetic beads or Ficoll.


RESULTS
T lymphocytes isolated by fluorobeads have better activity than that in mononuclear cells isolated by Ficoll. In order to get the more active cord blood cells, samples stored in vitro over 24 hours must be isolated with fluorobeads. Its serological reaction is lower as compared with that of peripheral mononuclear cells. Except 28 samples which had too many dead cells or few cells, there were 310 (17.5%) unconfirmed antigens among 1772 cases by serological typing. The main causes were low specificity of typing reagents, cross reaction, weak serological reaction, high background of dead cells and extra reaction. Confirmed by PCR-SSP, the serological typing had a wrong result rate of 13.88%.


CONCLUSIONS
Although serological typing is rapid and easy, it is necessary to use genetic typing as a complement to it.
Li
D
D
People's Hospital, Beijing Medical University, Beijing 100044, China.
Liu
H
H
Yu
Y
Y
Xi
B
B
D004247:DNA / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D015234:HLA-A Antigens / Q000097:blood* / Q000235:genetics / Q000276:immunology; D015235:HLA-B Antigens / Q000097:blood* / Q000235:genetics / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012698:Serologic Tests; D013601:T-Lymphocytes / Q000276:immunology
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D004247:DNA