[Studies on apoptosis of human lung adenocarcinoma cells induced by oltipraz].
OBJECTIVE
To study whether oltipraz can induce apoptosis of human lung adenocarcinoma (GLC-82) cells and its possible mechanism.


METHODS
Light microscopy, DNA electrophoresis, flow cytometry and video time-lapse monitoring were used to observe apoptosis of GLC-82 induced by oltipraz.


RESULTS
GLC-82 cells treated with oltipraz underwent the arrest of cell mitosis at metaphase, higher percentage of G(2)/M and peak of apoptosis were found, and cell body shrunk, nuclear chromosome condensed or fragmented and nuclear DNA fragment displayed "laden" bands in apoptic cells. Apoptosis of GLC-82 induced by oltipraz was more obvious at a concentration of 120 microg/ml.


CONCLUSIONS
Oltipraz could induce apoptosis of GLC-82 at certain concentrations, which associated closely with the arrest of mitotic cycle.
Bai
H
H
Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021.
Luo
X
X
Hu
G
G
Wei
H
H
Chen
F
F
Zhu
M
M
D000230:Adenocarcinoma / Q000473:pathology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008938:Mitosis / Q000187:drug effects; D011719:Pyrazines / Q000494:pharmacology*; D013871:Thiones; D013876:Thiophenes; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D011719:Pyrazines; D013871:Thiones; D013876:Thiophenes; C026209:oltipraz