Inexpensive holder for hand-trimming resin-embedded tissues.
A simple and inexpensive mount for securely holding resin-embedded tissues for trimming prior to sectioning for light or electron microscopy is described. The unit is composed of a base plate from which a 12.7-mm drill chuck protrudes. A tissue block is clamped into a microtome chuck and that is mounted in the drill chuck. A standard dissecting microscope is placed over the unit and used to magnify the block for trimming. Total cost of materials is estimated to be $20-40 USD.
Wise
Robert R
RR
Department of Biology, University of Wisconsin-Oshkosh, Oshkosh, Wisconsin 54901, USA. wise@uwosh.edu
D008854:Microscopy, Electron; D012116:Resins, Plant; D016610:Tissue Embedding / Q000295:instrumentation*
D012116:Resins, Plant