title: From anhydrobiosis to freeze-drying of eukaryotic cells.
abstract: Using what has been learned from nature, it has become possible to stabilize biological structures, including intact cells, in the dry state. Stabilization of cells or tissues in the dried state is of considerable practical significance, as is described in this review. The need for stabilization of cells in the dried state is particularly urgent in bloodbanks, where proper storage of blood cells (platelets and erythrocytes) is still a major problem. Human blood platelets are stored in blood banks for 5 days, after which they are discarded according to Federal regulation. This short lifetime has led to a chronic shortage of platelets. We report here that platelets can be preserved by freeze-drying them with trehalose, a sugar found at high concentrations in organisms that naturally survive drying. We suggest that this finding will obviate the storage problem with platelets and that the principles established here may be extended to more complex eukaryotic cells.
authors.affiliation: Department of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA. wfwolkers@ucdavis.edu
authors.forename: Willem F
authors.initials: WF
authors.lastname: Wolkers
authors.forename: Fern
authors.initials: F
authors.lastname: Tablin
authors.forename: John H
authors.initials: JH
authors.lastname: Crowe
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D005057:Eukaryotic Cells / Q000166:cytology*; D005612:Freeze Drying; D006801:Humans; D014199:Trehalose / Q000494:pharmacology*
chemical_list: D014199:Trehalose