Amphiphilic block copolymers as bile acid sorbents: 2. Polystyrene-b-poly(N,N,N-trimethylammoniumethylene acrylamide chloride): self-assembly and application to serum cholesterol reduction.
This paper presents morphological studies and preliminary bile salt binding properties of the new amphiphilic diblock copolymer polystyrene-b-poly(N,N,N-trimethylammoniumethylene acrylamide chloride) (PS-b-PTMEACl)(1) (see Figure 1), a derivative of PS-b-poly(tert-butylacrylate) (PS-b-PtBuA). In an aqueous environment, PS-b-PTMEACl forms simple spheres (approximately 20 nm diameter), large compound micelles (>100 nm diameter), and larger, more complex architectures as presented and discussed below. The colloidal stability with respect to sodium chloride and as a function of particle concentration is also considered. Finally, PS-b-PTMEACl aggregates were prepared and tested as an alternative to the commercially available bile salt sequestrant resins that target coronary heart disease due to elevated cholesterol levels. Electron micrographs were employed to visualize the colloid-based polyelectrolyteminus signbiosurfactant interaction and chromatographic separation analytical methods were used to quantify the sequestration. The results indicate that although at this preliminary stage they require laborious preparation, self-assembled aggregates may present an interesting alternative to the clinically used bile salt sequestrants.
Cameron
Neil S
NS
Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montréal, Québec, Canada H3A 2K6.
Eisenberg
Adi
A
Brown
G Ronald
GR
D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D002784:Cholesterol / Q000097:blood*; D003432:Cross-Linking Reagents; D007700:Kinetics; D008823:Micelles; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism*; D011137:Polystyrenes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012116:Resins, Plant / Q000493:pharmacokinetics / Q000494:pharmacology; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry*
D001647:Bile Acids and Salts; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D008823:Micelles; D011108:Polymers; D011137:Polystyrenes; D012116:Resins, Plant; D013501:Surface-Active Agents; D002784:Cholesterol