Protective immunity against Mycobacterium tuberculosis induced by dendritic cells pulsed with both CD8(+)- and CD4(+)-T-cell epitopes from antigen 85A.
Immunization with DNA followed by modified vaccinia virus Ankara strain, both expressing the antigen 85A, induced both CD4(+)- and CD8(+)-T-cell responses in BALB/c mice. Following challenge with Mycobacterium tuberculosis, this prime-boost regimen produced protection equivalent to that conferred by Mycobacterium bovis BCG. Following immunization with dendritic cells pulsed with an antigen 85A CD4(+)- or CD8(+)-restricted epitope, alone or in combination, copresentation of both epitopes on the same dendritic cell was required for protection, demonstrating that induced CD8(+) T cells can play a protective role against tuberculosis.
McShane
Helen
H
Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. helen.mcshane@ndm.ox.ac.uk
Behboudi
Shahriar
S
Goonetilleke
Nilu
N
Brookes
Roger
R
Hill
Adrian V S
AV
D000217:Acyltransferases; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000627:therapeutic use*; D001500:BCG Vaccine / Q000627:therapeutic use*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000637:transplantation*; D000939:Epitopes; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014376:Tuberculosis / Q000517:prevention & control*; D014611:Vaccination
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D000939:Epitopes; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis