title: Separation of human serum transferrins with different iron-binding states by high-performance liquid chromatography using a pyridinium polymer column.
abstract: Four molecular forms of transferrins with different iron-binding states were separated by HPLC using a pyridinium polymer column. The elution order was monoferric transferrin bound to the C-site, holotransferrin, apotransferrin and monoferric transferrin bound to the N-site. Human sera were also analyzed with the column, and ICP-MS combined with HPLC was used to detect iron in each peak. Transferrin peaks separated by HPLC were also confirmed by an immunological method. The percentages of iron saturation in transferrins obtained by the HPLC method were compared with the values calculated from clinical data.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kumamoto University, Japan. kumiko@gpo.kumamoto-u.ac.jp
authors.forename: Kumiko
authors.initials: K
authors.lastname: Harad
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kuniyasu
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakayama
authors.forename: Morio
authors.initials: M
authors.lastname: Nakayama
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Uji
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugiuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Okabe
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007501:Iron / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D011108:Polymers; D011485:Protein Binding; D011725:Pyridines / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D014168:Transferrin / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D011108:Polymers; D011725:Pyridines; D014168:Transferrin; D007501:Iron