title: Antiplasmodial activity of Uvaria klaineana.
abstract: Crude extracts of Uvaria klaineana Engler and Diels (Annonaceae) stems showed in vitro activity against chloroquine-resistant K1 strain of Plasmodium falciparum. The most active extract was the basic dichloromethane extract containing crude alkaloids (IC50 = 3.55 microg/mL). The bioassay-guided fractionation of this extract led to the isolation of the major alkaloid crotsparine (1) which showed an antiplasmodial activity against the chloroquine-sensitive Thai strain of P. falciparum and the chloroquine-resistant K1 and FcB1 strains of P. falciparum. Two minor alkaloids were also identified as crotonosine (2) and zenkerine (3). Their structures were elucidated using 2D-NMR techniques.
authors.forename: B
authors.initials: B
authors.lastname: Akendengue
authors.forename: E
authors.initials: E
authors.lastname: Ngou-Milama
authors.forename: F
authors.initials: F
authors.lastname: Roblot
authors.forename: A
authors.initials: A
authors.lastname: Laurens
authors.forename: R
authors.initials: R
authors.lastname: Hocquemiller
authors.forename: Ph
authors.initials: P
authors.lastname: Grellier
authors.forename: F
authors.initials: F
authors.lastname: Frappier
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D029061:Annonaceae; D000962:Antimalarials / Q000494:pharmacology*; D001681:Biological Assay; D002738:Chloroquine / Q000494:pharmacology; D004351:Drug Resistance; D005681:Gabon; D016488:Medicine, African Traditional; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D010963:Plasmodium falciparum / Q000187:drug effects*
chemical_list: D000470:Alkaloids; D000962:Antimalarials; D010936:Plant Extracts; C455069:crotonosine; C455068:crotsparine; C455070:zenkerine; D002738:Chloroquine