Abnormal FHIT expression profiles in cervical intraepithelial neoplastic (CIN) lesions.
Abnormal fragile histidine triad transcripts were found in 20-30% of CIN2/3 lesions and 11% of normal cervical biopsies by RT-PCR. Bi-allelic loss of the fragile histidine triad gene and the loss of fragile histidine triad protein expression detectable by immunochemical staining with a polyclonal fragile histidine triad specific antibody was rare. The genomic changes showed no association with the presence of human papillomavirus types which carry high risk for cervical cancer (high risk human papillomavirus) as assessed by a type-specific multiplex PCR. The presence of abnormal fragile histidine triad transcripts in a subset of CIN2/3 lesions with no high risk human papillomavirus suggests that this could be an independent risk factor associated with an alternative carcinogenic pathway.
Terry
G
G
Department of Molecular Pathology, Windeyer Building, University College London, 46 Cleveland Street, London W1T 4JF, UK.
Ho
L
L
Londesborough
P
P
Cuzick
J
J
D017766:Acid Anhydride Hydrolases; D017398:Alternative Splicing; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D009367:Neoplasm Staging; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000235:genetics* / Q000473:pathology; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase