title: [Study of graft-versus -leukemia activity of gamma-ray irradiated haplomismatched donor lymphocytes infusion].
abstract: OBJECTIVE
  To explore graft-versus-leukemia activity of 7.5 Gy gamma-ray irradiated haplomismatched donor lymphocytes infusion (DLI).
  
  
  METHODS
  L615 cells loaded 615 (H2(k))/BALB/c (H-2(d)) F(1) mice were infused with irradiated haplo-mismatched donor lymphocytes at 3, 5, 7 days after CTX (200 mg/kg) treatment which was given at the 3rd day after L615 cells inoculation.
  
  
  RESULTS
  T-cells irradiated with 7.5 Gy gamma-ray lost their proliferative capacity but preserved their cytotoxic activity against allogeneic targets. Survival of these recipient mice was longer than that of control (t = 10.03, P < 0.001) with no severe graft-versus-host disease (GVHD).
  
  
  CONCLUSIONS
  7.5 Gy gamma irradiation attenuated the GVHD inducing potential of allogeneic lymphocytes while preserving their graft-versus-leukemia activity. It provides a simple modality to postpone and prevent leukemia relapse.
authors.affiliation: Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Guo
authors.forename: B
authors.initials: B
authors.lastname: Dong
authors.forename: Q
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D005720:Gamma Rays / Q000627:therapeutic use*; D006086:Graft vs Host Disease / Q000517:prevention & control; D020544:Graft vs Leukemia Effect / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000528:radiation effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D014019:Tissue Donors