[Genoprotective human blood activity and its mechanisms].
Anticlastogenic properties of plasma and proteins (albumin and gamma-globulin) of the human blood were studied using seeds of Crepis capillaris (chromosome aberration assay). Antimutagen p-amino-benzoic acid was used as a comparative reagent. Anticlastogenic activity dependent of processing conditions of the biosubstrate used; for the pre-processing and combined processing anticlastogenic effect was higher than for post-processing, the processing properties of the blood being higher than those of the blood proteins. Anticlastogenic potential of biosubstrates did not depend on mutation inductor. Complex-formimg properties of plasma and blood albumen have been revealed using spectrop-hotometry through the substantial spectral displacement--relative to the expected spectrum--for the mixture of biosubstrata and mutagens. Using chemoluminescence, all plasma, albumin and gamma-globulin concentrations have been shown to enhance generation of hydroxyl radical of the Fenton reagent, especially for albumin in 1.0 g/l concentration. The general trend for all experiments was that the said substances diminished the stimulating effect as their concentrations grew. Peroxidation of yolk lipoproteids showed that only high concentrations of blood's plasma and albumen have antioxidizing properties. gamma-Globulin did not reveal any ability to inhibit lipid peroxidation of yolk lipoproteids. Complex-forming mechanisms of blood's albumen and antioxidizing property of human plasma and proteins have been proved to form the blood's anticlastogenic potential.
Koshpaeva
E S
ES
Kazan State Medical University, 420012, Kazan, 49 Butlerov St., Russia.
Semenov
V V
VV
Semenov
A V
AV
D000418:Albumins / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D001769:Blood; D002869:Chromosome Aberrations / Q000139:chemically induced; D027468:Crepis; D005020:Ethyl Methanesulfonate; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D009152:Mutagenicity Tests; D009153:Mutagens; D010949:Plasma; D005719:gamma-Globulins / Q000494:pharmacology
D000418:Albumins; D016587:Antimutagenic Agents; D009153:Mutagens; D005719:gamma-Globulins; D017665:Hydroxyl Radical; D005020:Ethyl Methanesulfonate