title: Comparison of cryopreservation outcome with gonadotropin-releasing hormone agonists or antagonists in the collecting cycle.
abstract: OBJECTIVE
  To compare the pregnancy rates of frozen-thawed 2-pronucleate (2PN) oocytes obtained either in a long protocol or in an antagonist protocol and ovarian stimulation with either human menopausal gonadotropin (hMG) or recombinant follicular stimulating hormone (recFSH).
  
  
  METHODS
  Retrospective data analysis.
  
  
  METHODS
  Academic infertility center.
  
  
  METHODS
  Three hundred forty-two infertile couples who underwent a transfer of cryopreserved 2PN oocytes.
  
  
  METHODS
  hMG (n = 194) or recFSH (n = 92) in a long protocol or hMG (n = 16) or recFSH (n = 40) stimulation under pituitary suppression with the GnRH antagonist Cetrotide was used. The 2PN oocytes were transferred after endometrial preparation using E(2) valerate and vaginal progesterone (Crinone 8% vaginal gel).
  
  
  METHODS
  Implantation, pregnancy, and abortion rates.
  
  
  RESULTS
  Implantation rates in the freeze-thaw cycles were 5.6% (hMG) and 3.8% (recFSH) with 2PN oocytes from the long protocol and 7% from the antagonist cycles, irrespective of whether hMG or recFSH was used. Pregnancy rates were similar independent of whether they resulted from the long-protocol cycles with hMG (15.4%) and recFSH (13.1%) or from the antagonist protocol cycles with hMG (25.0%) and recFSH (17.5%).
  
  
  CONCLUSIONS
  The potential to implant is independent of the gonadotropin-releasing hormone analogue and gonadotropin chosen for the collection cycle when previously cryopreserved 2PN oocytes were replaced after thawing in the cleavage stage.
authors.affiliation: Department of Gynecology and Obstetrics, University Clinic Hospital, Lübeck, Germany. aseeling@hotmail.com
authors.forename: Anna Sophie
authors.initials: AS
authors.lastname: Seelig
authors.forename: Safa
authors.initials: S
authors.lastname: Al-Hasani
authors.forename: Alexander
authors.initials: A
authors.lastname: Katalinic
authors.forename: Beate
authors.initials: B
authors.lastname: Schöpper
authors.forename: Rita
authors.initials: R
authors.lastname: Sturm
authors.forename: Klaus
authors.initials: K
authors.lastname: Diedrich
authors.forename: Michael
authors.initials: M
authors.lastname: Ludwig
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006727:Hormone Antagonists / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009865:Oocytes; D010062:Ovulation Induction / Q000379:methods*; D011247:Pregnancy; D018873:Pregnancy Rate; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D006727:Hormone Antagonists; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D005640:Follicle Stimulating Hormone; C062876:cetrorelix