Modulation of rat liver cytochrome P450 activity by prolonged red wine consumption.
Cytochrome P450-dependent oxidation of lauric acid, p-nitrophenol and ethanol by liver microsomal fractions were studied in control rats and in animals given either ethanol, red wine, or alcohol-free red wine for 10 weeks. Ethanol increased the total cytochrome P450 and the isoenzyme 2E1 content, as well as the p-nitrophenol hydroxylation and ethanol oxidation. These effects of ethanol treatment were attenuated by red wine administration. Red wine increased the total antioxidant capacity of plasma, whereas the alcohol-free red wine decreased the cytochrome P450 content and decreased the oxidation of lauric acid, p-nitrophenol and ethanol to values lower than control. It is concluded that red wine administration attenuates the ethanol-induced enhancement in liver microsomal parameters dependent on cytochrome P450 2E1 activity, an affect that seems to be accomplished by the non-alcoholic constituents of red wine known to have antioxidant properties.
Orellana
Myriam
M
ICBM, Programa de Farmacología Molecular y Clínica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile. morellan@machi.med.uchile.cl
Varela
Nelson
N
Guajardo
Viviana
V
Araya
Julia
J
Rodrigo
Ramón
R
D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D000431:Ethanol / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D005419:Flavonoids; D007850:Lauric Acids / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D010084:Oxidation-Reduction; D010636:Phenols / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism; D011108:Polymers / Q000008:administration & dosage / Q000378:metabolism; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014920:Wine
D000975:Antioxidants; D005419:Flavonoids; D007850:Lauric Acids; D009596:Nitrophenols; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; C030358:lauric acid; D000431:Ethanol; D019392:Cytochrome P-450 CYP2E1; C024836:4-nitrophenol