Syrian hamster 5S rRNA genes are assigned to 6q2 with PNA-FISH by an R-banded karyotype.
A karyotype for the Syrian hamster is proposed based on an R-banding pattern. R-bands were obtained by BrdU incorporation into the cells followed by a combined DAPI and propidium iodide staining of the fixed metaphase spreads. In situ hybridisation was performed with two biotinylated 18-mer PNA (peptide nucleic acid) probes complementary to sequences within the 5S rRNA gene. The 5S rRNA gene repeats map to chromosome 6q2. The present PNA-FISH procedure is an abbreviated and simpler version of that previously published.
Lomholt
B
B
Department of Medical Biochemistry and Genetics, Biochemistry Laboratory B, The Panum Institute, University of Copenhagen, Denmark.
Christensen
K
K
Frederiksen
S
S
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics*; D006224:Cricetinae; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007211:Indoles / Q000378:metabolism; D007621:Karyotyping; D008647:Mesocricetus / Q000235:genetics*; D015335:Molecular Probes / Q000235:genetics / Q000378:metabolism*; D020135:Peptide Nucleic Acids / Q000235:genetics / Q000378:metabolism*; D020161:Physical Chromosome Mapping / Q000379:methods; D011419:Propidium / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
D007211:Indoles; D015335:Molecular Probes; D020135:Peptide Nucleic Acids; D012341:RNA, Ribosomal, 5S; D011419:Propidium; C007293:DAPI; D001973:Bromodeoxyuridine