title: Induction of chemokines in rat pancreatic acinar cell injury.
abstract: BACKGROUND
  The pathogenesis of chronic pancreatitis (CP), especially of acinar cell injury, is still unclear. Interleukin (IL)-8 is a chemokine that is involved in various inflammatory diseases.
  
  
  OBJECTIVE
  To examine whether IL-8 and other chemokines are expressed in experimental acinar cell injury.
  
  
  METHODS
  IL-8 expression was analyzed in spontaneous CP in the WBN/Kob rat and in rat pancreatic acinar AR4-2J cells treated with various stimuli using reverse transcription-polymerase chain reaction (semiquantitative) and immunohistochemistry.
  
  
  RESULTS
  Chronic pancreatitis developed at 12 weeks in the WBN/Kob rats. IL-8, macrophage chemoattractant protein-1, and macrophage inflammatory protein-2 mRNA was expressed from 4 weeks and peaked at 12 weeks. Immunohistochemistry showed a strong expression of IL-8 in acinar cells, proliferating ductular cells, and interstitial infiltrating cells. In contrast, normal pancreatic tissues lacked IL-8 expression. Further, IL-8 mRNA and protein were detectable in AR4-2J cells treated with the various stimuli, such as menadione, tumor necrosis factor-alpha and transforming growth factor beta1.
  
  
  CONCLUSIONS
  These results suggest that IL-8 is expressed in the pancreatic parenchyma and infiltrates in CP and that it plays a role in the initial pathogenesis of CP together with other chemokines and cytokines.
authors.affiliation: Department of Internal Medicine and Medical Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-0934, Japan.
authors.forename: Min-Jue
authors.initials: MJ
authors.lastname: Xie
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Motoo
authors.forename: Shi-Bing
authors.initials: SB
authors.lastname: Su
authors.forename: Hisatsugu
authors.initials: H
authors.lastname: Mouri
authors.forename: Norio
authors.initials: N
authors.lastname: Sawabu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000235:genetics; D054426:Chemokine CXCL2; D018925:Chemokines / Q000235:genetics; D002908:Chronic Disease; D004195:Disease Models, Animal; D015870:Gene Expression / Q000276:immunology; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000032:analysis / Q000235:genetics*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D010179:Pancreas / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D010195:Pancreatitis / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018932:Chemokine CCL2; D054426:Chemokine CXCL2; D018925:Chemokines; C404915:Cxcl2 protein, mouse; D016209:Interleukin-8; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat