title: Chemical incorporation of 1-methyladenosine into oligonucleotides.
abstract: The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.
authors.affiliation: Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Mikhailov
authors.forename: Jef
authors.initials: J
authors.lastname: Rozenski
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Efimtseva
authors.forename: Roger
authors.initials: R
authors.lastname: Busson
authors.forename: Arthur
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: Piet
authors.initials: P
authors.lastname: Herdewijn
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006358:Hot Temperature; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis
chemical_list: D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; C002230:1-methyladenosine; D000241:Adenosine