Development of a sandwich ELISA and comparison with PCR for the detection of F11 and F165 fimbriated Escherichia coli isolates from septicaemic disease in farm animals.
The P fimbriae F11 and F165 that have been demonstrated on Escherichia coli septicaemic strains in poultry and calves, respectively, possess a nearly identical major subunit that demonstrates a serological cross-reaction. A polyclonal antibody-based sandwich ELISA (sELISA) that was specific for both F11 and F165 fimbriated strains was compared with a PCR method to detect F11/F165 fimbriated strains, in a collection of E. coli strains isolated from diseased animals. Of 298 isolates tested, 36 were positive by PCR of which only 14 were sELISA positive. There were no sELISA positive but PCR negative results. The 36 PCR positive isolates comprised 11 avian strains of which 10 were sELISA positive, 20 bovine strains of which 4 were sELISA positive and 3 ovine strains, 1 porcine strain and 1 equine strain all of which were sELISA negative. The F11/F165 incidence of 10.7% in 103 poultry and 18.3% in 109 bovine isolates demonstrates a moderate level of these factors in E. coli septicaemic cases in Northern Ireland.
Bell
C J
CJ
Department of Agriculture and Rural Development, Veterinary Sciences Division, Stoney Road, Stormont, BT4 3SD, Northern Ireland, Belfast, UK. colinjbell@yahoo.co.uk
Finlay
D A
DA
Clarke
H J
HJ
Taylor
M J
MJ
Ball
H J
HJ
D000818:Animals; D000829:Animals, Domestic; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000276:immunology; D006736:Horses; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012756:Sheep; D013552:Swine