CK2(beta)tes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster.
An earlier described CK2(beta)tes gene of Drosophila melanogaster is shown to encode a male germline specific isoform of regulatory beta subunit of casein kinase 2. Western-analysis using anti-CK2(beta)tes Ig revealed CK2(beta)tes protein in Drosophila testes extract. Expression of a CK2(beta)tes-beta-galactosidase fusion protein driven by the CK2(beta)tes promoter was found in transgenic flies at postmitotic stages of spermatogenesis. Examination of biochemical characteristics of a recombinant CK2(beta)tes protein expressed in Escherichia coli revealed properties similar to those of CK2beta: (a) CK2(beta)tes protein stimulates CK2alpha catalytic activity toward synthetic peptide; (b) it inhibits phosphorylation of calmodulin and mediates stimulation of CK2alpha by polylysine; (c) it is able to form (CK2(beta)tes)2 dimers, as well as (CK2alpha)2(CK2(beta)tes)2 tetramers. Using the yeast two-hybrid system and coimmunoprecipitation analysis of protein extract from Drosophila testes, we demonstrated an association between CK2(beta)tes and CK2alpha. Northern-analysis has shown that another regulatory (beta') subunit found recently in D. melanogaster genome is also testis-specific. Thus, we describe the first example of two tissue-specific regulatory subunits of CK2 which might serve to provide CK2 substrate recognition during spermatogenesis.
Kalmykova
Alla I
AI
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Shevelyov
Yuri Y
YY
Polesskaya
Oksana O
OO
Dobritsa
Anna A
AA
Evstafieva
Alexandra G
AG
Boldyreff
Brigitte
B
Issinger
Olaf-Georg
OG
Gvozdev
Vladimir A
VA
D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D047390:Casein Kinase II; D002384:Catalysis; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D009928:Organ Specificity; D011107:Polylysine / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D021122:Protein Subunits; D012097:Repressor Proteins / Q000235:genetics; D013737:Testis / Q000201:enzymology; D020798:Two-Hybrid System Techniques
D002147:Calmodulin; D029721:Drosophila Proteins; D007527:Isoenzymes; D021122:Protein Subunits; D012097:Repressor Proteins; C106547:Ssl protein, Drosophila; D011107:Polylysine; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases