Water deficit during root development: effects on the growth of roots and osmotic water permeability of isolated root protoplasts.
The effect of low water potentials on root growth of flax (Linum usitatissimum L. cv. Ariane), rape (Brassica napus L. de Candolle, cv. Bristol), hard wheat (Triticum turgidum L. cv. Cham1) and soft wheat (Triticum aestivum L. cv. Ritmo) was studied by measuring the osmotic water permeability (Pos) of root protoplasts and the protein abundance of PIP1 and PIP2 aquaporins. These different species require more or less water, the most sensitive to water deficit being flax and rape. Ritmo, is a cultivar of wheat adapted to temperate zones, while the other cultivar Cham1 is adapted to low-rainfall areas. The seedlings were germinated and grown in water, salt or sugar solutions at different water potentials. The values of Pos for flax, rape and Chaml wheat were normally distributed and could be characterized by mean +/- SD. Root protoplasts from water-grown seedlings had Pos values of 485+/-159 microm s(-1) (flax), 582+/-100 microm s(-1) (rape), and 6.3+/-3.5 microm s(-1) (Cham1). At the same age, the protoplasts from Ritmo exhibited a much wider range of values than the protoplasts of Cham1. When seedlings were grown under conditions of osmotic or salt stress, the mass of the roots was reduced for all species. With 0.25 mol kg(-1) sorbitol or 0.125 M NaCl, the Pos for flax, rape and Cham1 remained constant or slightly increased, while for Ritmo the reduction in the mass of the roots was paralleled by a reduction in Pos. Only Cham1 and Ritmo were able to germinate at a lower potential (0.5 mol kg(-1) sorbitol). For Ritmo the reduction in the mass of the roots was paralleled by a reduction in Pos when grown in this stress condition and both wheats exhibited low Pos values. The expression of the PIP1 and PIP2 aquaporins families was also studied by immunoblotting. We did not observe any difference in protein expression for any of the species, whatever the growing conditions. We suggest that the high Pos values for flax and rape could play a role in the sensitivity of these plants at low water potential. The low native Pos for Cham1 in spite of the expression of both families of aquaporins may reflect its adaptation to low-rainfall conditions by a functional regulation of the water channels. For a similar reason, the low-water-potential-induced Pos of Ritmo may also correspond to a down-regulation of the aquaporins, reflecting adaptation of this wheat to water-deficit conditions.
Morillon
Raphaël
R
UMR CNRS 6037, Université de Rouen, Faculté des Sciences, Mont-Saint-Aignan, France.
Lassalles
Jean-Paul
JP
D000222:Adaptation, Physiological / Q000502:physiology; D020346:Aquaporins / Q000378:metabolism; D029688:Brassica napus / Q000187:drug effects / Q000254:growth & development; D002463:Cell Membrane Permeability / Q000502:physiology*; D002478:Cells, Cultured; D019597:Flax / Q000187:drug effects / Q000254:growth & development; D015151:Immunoblotting; D019684:Magnoliopsida / Q000187:drug effects / Q000254:growth & development*; D009997:Osmotic Pressure / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D011523:Protoplasts / Q000187:drug effects*; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects / Q000254:growth & development; D014867:Water / Q000378:metabolism* / Q000494:pharmacology
D020346:Aquaporins; D014867:Water; D012965:Sodium Chloride; D013012:Sorbitol