title: Outer and central charged residues in DIVS4 of skeletal muscle sodium channels have differing roles in deactivation.
abstract: We tested the effects of charge-neutralizing mutations of the eight arginine residues in DIVS4 of the rat skeletal muscle sodium channel (rNa(V)1.4) on deactivation gating from the open and inactivated states. We hypothesized that neutralization of outer or central charges would accelerate the I-to-C transition as measured by recovery delay because these represent a portion of the immobilizable charge. R1Q abbreviated recovery delay as a consequence of reduced charge content. R4Q increased delay, whereas R5Q abbreviated delay, and charge-substitutions at these residues indicated that each effect was allosteric. We also hypothesized that neutralization of any residue in DIVS4 would slow the O-to-C transition with reduction in positive charge. Reduction in charge at R1, and to a lesser extent at R5, slowed open-state deactivation, while charge neutralizations at R2, R3, R4, R6, and R7 accelerated open-state deactivation. Our findings suggest that arginine residues in DIVS4 in rNa(V)1.4 have differing roles in channel closure from open and inactivated states. Furthermore, they suggest that deactivation in DIVS4 is regulated by charge interaction between the electrical field with the outermost residue, and by local allosteric interactions imparted by central charges.
authors.affiliation: Department of Biology, Harvey Mudd College, Claremont, California 91711, USA.
authors.forename: James
authors.initials: J
authors.lastname: Groome
authors.forename: Esther
authors.initials: E
authors.lastname: Fujimoto
authors.forename: Lisa
authors.initials: L
authors.lastname: Walter
authors.forename: Peter
authors.initials: P
authors.lastname: Ruben
mesh_terms: D000495:Allosteric Site; D000818:Animals; D001120:Arginine / Q000737:chemistry; D004594:Electrophysiology; D007700:Kinetics; D018482:Muscle, Skeletal / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051381:Rats; D015222:Sodium Channels / Q000737:chemistry* / Q000502:physiology; D013997:Time Factors; D014982:Xenopus laevis
chemical_list: D015222:Sodium Channels; D001120:Arginine