Geraniol-inducible glutathione S-transferase in cultured soybean cells.
When the cultured cells of Glycine max (soybean) were treated with 5 mM geraniol as a chemical stress, an mRNA level was elevated in a rapid but transient increase. The mRNA was cloned and sequenced, and found to correspond to the mRNA encoding glutathione S-transferase (GST). The GST mRNA level and GST activity were elevated to maxima at 4-6 h and 8 h, respectively, after treatment of the cultures with geraniol. These indicate that GST is one of the geraniol-responsive factors in soybean cells.
Ashida
Yoshiyuki
Y
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Matsushima
Akihito
A
Hirota
Tomoko
T
Watanabe
Junko
J
Hirata
Toshifumi
T
D000080462:Acyclic Monoterpenes; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology*; D013729:Terpenes / Q000494:pharmacology*
D000080462:Acyclic Monoterpenes; D018076:DNA, Complementary; D012333:RNA, Messenger; D013729:Terpenes; D005982:Glutathione Transferase; C007836:geraniol