Polygalacturonase production by Aspergillus awamori on wheat in solid-state fermentation.
The production of exo-polygalacturonase (exo-PG) and endo-PG by Aspergillus awamori grown on wheat in solid-state fermentation was studied. Endo- and exo-PG activities were detected after 24 h of inoculation. Glucose released from starch hydrolysis acted as a catabolite repressor for the exo-PG enzyme. In contrast, endo-PG production was not affected by glucose repression. When milled grains were used, the particle-size distribution and the chemical composition of the medium influenced the rate of micro-organism growth and therefore the trend followed by endo- and exo-PG production. However, these two parameters did not affect the maximum production of exo-PG and endo-PG. For one of the milled samples, three different moisture contents were used (50, 55, 60%). Moisture contents of 60% provide a higher yield of pectinases by A. awamori.
Blandino
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Department of Chemical Engineering, Faculty of Sciences, University of Cádiz, Puerto Real, Spain. s.pandiella@umist.ac.uk
Iqbalsyah
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T
Pandiella
S S
SS
Cantero
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D
Webb
C
C
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D011096:Polygalacturonase / Q000096:biosynthesis*; D014908:Triticum / Q000378:metabolism
D003470:Culture Media; D011096:Polygalacturonase