[The role of bFGF in renal tubular injury and the interstitial lesion].
OBJECTIVE
The role of bFGF in renal tubular injury, regeneration and interstitial lesion of rats was studied.


METHODS
The renal tubular injury model of rat was induced by intraperitoneal injection of gentamicin. The expression of bFGF and FGFR1 was examined by Northern blot, in situ hybridization and immunohistochemistry. (3)H-TdR incorporation was used to detect the role of bFGF in promoting the proliferation of renal tubular epithelial cells and renal fibroblasts.


RESULTS
The expression of bFGF mRNA and protein synthesis of bFGF and FGFR1 increased during the process of injury and regeneration of tubular epithelial cells. In contrast, normal tubular epithelium failed to express bFGF, except renal interstitial fibroblasts and monocytes/macrophages. bFGF promoted proliferation of renal tubular epithelium as well as renal interstitial fibroblasts.


CONCLUSIONS
bFGF is secreted by tubular epithelial cells damaged by nephrotoxicity of gentamicin, and FGFR1 expressed in damaged renal tubules and interstitial fibroblasts. bFGF not only can promote tubular epithelia to proliferate, but can also stimulate interstitial fibroblasts to proliferate. Correlation exists between bFGF, renal tubular injury and interstitial lesions.
Du
A
A
Department of Pathology, Beijing Medical University, Beijing 100083, China.
Zou
W
W
D000818:Animals; D002455:Cell Division; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology; D005839:Gentamicins; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D051496:Receptor, Fibroblast Growth Factor, Type 1; D017468:Receptors, Fibroblast Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012038:Regeneration
D005839:Gentamicins; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; C496350:Fgfr1 protein, rat; D020794:Receptor Protein-Tyrosine Kinases; D051496:Receptor, Fibroblast Growth Factor, Type 1