[Human cytomegalovirus inhibits the proliferation of CFU-MK in vitro].
OBJECTIVE
To investigate the effect of human cytomegalovirus (HCMV) on the proliferation of colony forming unit-megakaryocyte (CFU-MK).


METHODS
Semi-solid CFU-MK culture system was used to observe the effect of HCMV AD169 strain on CFU-MK growth of 20 cord blood samples. HCMV DNA and immediate early antigen (IEA) mRNA in CFU-MK were detected by in situ-polymerase chain reaction (IS-PCR) and reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
HCMV AD169 suppressed the differentiation and proliferation of CFU-MK in vitro significantly. The suppression was in a dose-dependent fashion. HCMV DNA was successfully detected in colony cells from viral infection group, and did the expression of HCMV IEA mRNA.


CONCLUSIONS
HCMV AD169 can directly infect megakaryocyte progenitor and suppress their proliferation and differentiation.
Pan
S
S
Department of Pediatrics, The Third Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou, 510630, China.
He
Z
Z
Chen
F
F
Wang
Q
Q
Zou
X
X
Huang
L
L
Yang
M
M
D049109:Cell Proliferation; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000503:physiopathology* / Q000821:virology; D005260:Female; D006801:Humans; D055015:Megakaryocyte-Erythroid Progenitor Cells / Q000166:cytology* / Q000821:virology