title: Detection of Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii and group VS116 by PCR in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans.
abstract: OBJECTIVE: To evaluate the diagnostic performance of two polymerase chain reaction (PCR) procedures using skin biopsies of 20 erythema migrans (EM) and 24 acrodermatitis chronica atrophicans (ACA) patients. METHODS: One assay amplified a fragment of the outer surface protein (Osp) A gene. The second method amplified the spacer region between the 5S and 23S rRNA genes; hybridization of this fragment allowed identification of Borrelia burgdorferi sensu lato species. RESULTS: Among EM patients, both assays detected Borrelia DNA in 15 samples. Among ACA patients, the ospA PCR detected 15 positives and 10 samples were positive by 5S-23S PCR. In 19 samples one species was detected, 15 skin biopsies contained Borrelia afzelii, and Borrelia garinii was found in two patients. Group VS116 was detected in two EM patients, and therefore this group has pathogenic potential. Mixed infections of B. afzelii and B. garinii, group VS116 or B. burgdorferi sensu stricto were found in three EM and three ACA patients. CONCLUSIONS: Diagnosis of EM and ACA by PCR is useful and knowledge of the presence of species may be used to predict the course of disease or the need for further antibiotics.
authors.affiliation: Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands.
authors.forename: Sjoerd G. T.
authors.initials: SG
authors.lastname: Rijpkema
authors.forename: Dig J.
authors.initials: DJ
authors.lastname: Tazelaar
authors.forename: Marc J.C.H.
authors.initials: MJ
authors.lastname: Molkenboer
authors.forename: Gerda T.
authors.initials: GT
authors.lastname: Noordhoek
authors.forename: Grietsje
authors.initials: G
authors.lastname: Plantinga
authors.forename: Leo M.
authors.initials: LM
authors.lastname: Schouls
authors.forename: Joop F.P.
authors.initials: JF
authors.lastname: Schellekens