[The inhibitory effect of antisense adenovirus vector of telomerase on telomerase activity in breast cancer cells MCF-7].
OBJECTIVE
To inhibit telomerase activity in breast cancer cells MCF-7 by antisense ribonucleotide technique.


METHODS
Recombinant adenovirus was chosen as gene delivery vector to transfer and express the antisense RNA component of telomerase in tumor cells. The recombinant adenovirus of anti-telomerase RNA was obtained through gene recombination and co-transfection with lipofectin. The integrated potential of antisense virus was certified by Southern blot and the telomerase activity of MCF-7 was analysed by TRAP-silver staining.


RESULTS
The MCF-7 cell line is a typical model for malignant breast cancer. In this study, the telomerase activity of MCF-7 was significantly inhibited after antisense virus infection when compared with the control cell lines.


CONCLUSIONS
The introduction of antisense cDNA of telomerase RNA can significantly inhibit the activity of telomerase in MCF-7 cells.
Zhang
X
X
Beijing Medical University, Beijing 100853, China.
Tong
T
T
D000256:Adenoviridae / Q000235:genetics*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007668:Kidney / Q000166:cytology; D016372:RNA, Antisense / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D016372:RNA, Antisense; D019098:Telomerase