title: Misoprostol Modulation of Dendritic Cell Function in Immune Responses.
abstract: Dendritic cells (DC) from blood and other tissues are potent accessory cells for primary immune responses. Because prostaglandins from monocytes and macrophages can suppress DC and T-cell function, we sought to investigate the binding properties of misoprostol (MPL), a prostaglandin E(1) analog, and its regulation of DC function. Results of mouse and human experiments have suggested 1) that MPL could significantly inhibit DC-induced T-cell proliferation in oxidative mitogenesis and allogeneic mixed leukocyte reactions by decreasing interleukin-2 production in DC-T cell cocultures, and 2) that MPL could bind to human peripheral blood mononuclear cells via specific high-affinity MPL binding sites as well as through nonspecific MPL uptake. Taken together, these data suggest that MPL can bind high-affinity and/or nonspecific cell surface receptors and subsequently regulate T-cell growth and cytokine production such as that induced by DC and associated with primary immune responses.
authors.affiliation: Department of Medicine, The University of Alabama at Birmingham, and Veterans Affairs Medical Center, Birmingham, USA.
authors.forename: Michael P.
authors.initials: MP
authors.lastname: Everson
authors.forename: Anand
authors.initials: A
authors.lastname: Lagoo
authors.forename: Deborrah S.
authors.initials: DS
authors.lastname: McDuffie
authors.forename: Dennis J.
authors.initials: DJ
authors.lastname: Pillion