title: A rapid and sensitive high-performance liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry method for the quantitation of oxycodone in human plasma.
abstract: A sensitive method for the determination of oxycodone concentrations in plasma by high-performance liquid chromatography (HPLC)-electrospray ionization-triple quadrupole mass spectrometry is described. The method is rugged, reliable, selective, and rapid with a run time of 2 min. One milliliter of plasma is made basic and extracted with 2-mL duplicate portions of 2% isoamyl alcohol in n-butyl chloride. The combined extracts are then evaporated to dryness, reconstituted in 100 microL of the mobile phase (15% methanol-85% water containing 0.1% acetic acid), and injected onto the HPLC. The limit of quantitation is 1 ng/mL, and the estimated limit of detection is 33 pg/mL (signal-to-noise = 3). Standard curves are linear over the range of 1 to 100 ng/mL with all correlation coefficient values greater than 0.9989. The method is used to determine the concentration of oxycodone in human plasma following the intravenous infusion of doses ranging from 5 to 15 mg in which the analysis of over 3000 plasma samples is required.
authors.affiliation: Department of Chemistry, Materials and Forensic Science, University of Technology, Sydney, Broadway, NSW, Australia. michael.dawson@uts.edu.au
authors.forename: Michael
authors.initials: M
authors.lastname: Dawson
authors.forename: Bronwyn
authors.initials: B
authors.lastname: Fryirs
authors.forename: Tamsin
authors.initials: T
authors.lastname: Kelly
authors.forename: Jim
authors.initials: J
authors.lastname: Keegan
authors.forename: Laurence E
authors.initials: LE
authors.lastname: Mather
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010098:Oxycodone / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000701:Analgesics, Opioid; D010098:Oxycodone