Restricted-access media high pressure liquid chromatography vs fluorescence polarization immunoassay for analysis of carbamazepine in human plasma.
OBJECTIVE
To compare restricted-access media high performance liquid chromatographic (RAM-HPLC) method with fluorescence polarization immunoassay (FPIA) for analysis of carbamazepine (CBZ) in human blood.


METHODS
An RAM-HPLC method was established for the determination of CBZ in plasma.


RESULTS
The two methods do not need sample clean-up prior to analysis and they have almost 100 % recovery and good reproducibility. There is a good correlation between the CBZ concentration in venous plasma samples determined by FPIA and that in both venous and fingertip plasma samples obtained by RAM-HPLC, the correlation coefficients being 0.989 and 0.995, respectively. It is shown by t-test that the data sets of venous and fingertip plasma samples given by RAM-HPLC are consistent with each other but significantly different from the results obtained by FPIA.


CONCLUSIONS
Both direct injection RAM-HPLC and FPIA may be applied in determining CBZ in therapeutic drug monitoring (TDM). FPIA is well-suited to the routine TDM. RAM-HPLC is more useful in TDM related research and especial cases.
Ma
Jun
J
Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Zhu
Peng-Ling
PL
Xie
Jing-Wen
JW
Jia
Zheng-Ping
ZP
Yang
Jin-Sheng
JS
D000927:Anticonvulsants / Q000097:blood; D002220:Carbamazepine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D004827:Epilepsy / Q000097:blood / Q000188:drug therapy; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D006801:Humans
D000927:Anticonvulsants; D002220:Carbamazepine