title: Purification of Food Color Red No. 106 (acid red) using pH-zone-refining counter-current chromatography.
abstract: pH-Zone-refining counter-current chromatography was successfully applied to the separation of the main components of Food Color Red No. 106 (R-106, acid red, Color Index No. 45100). A 300-mg quantity of sample was separated using the following two-phase solvent system: n-butanol-water, 40 mM sulfuric acid in organic stationary phase and 30 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and fast atom bombardment mass spectrometry. The separation yielded 261.9 mg of main component of acid red with purity of 99.9%.
authors.affiliation: Aichi Prefectural Institute of Public Health, Nagoya, Japan. hisaooka@alles.or.jp
authors.forename: Hisao
authors.initials: H
authors.lastname: Oka
authors.forename: Masanao
authors.initials: M
authors.lastname: Suzuki
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Harada
authors.forename: Masato
authors.initials: M
authors.lastname: Iwaya
authors.forename: Kiyonaga
authors.initials: K
authors.lastname: Fujii
authors.forename: Tomomi
authors.initials: T
authors.lastname: Goto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012235:Rhodamines / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D012235:Rhodamines; C022027:lissamine rhodamine B