Evaluation of alpha-tocopherol, probucol and ascorbic acid as suppressors of digoxin induced lipid peroxidation.
Protective effects of three free radical scavengers, tocopherol (TOC), probucol (PR) and ascorbic acid (AA), on cardiotonic glycoside digoxin (DIG) induced lipid peroxidation in goat liver homogenate, have been studied by measuring malondialdehyde and glutathione contents as indicator parameters. The level of reduced glutathione decreased vis-a-vis malondialdehyde content increased in the drug treated samples in comparison with the controls. This suggests that DIG may have significant lipid peroxidation induction capacity. Considering lipid peroxidation as a toxicity mediating process, this may be related to the toxic potential of the drug. When the liver homogenate samples were incubated with antioxidant (TOC/PR/AA) in conjunction with the drug (DIG), lipid peroxidation was suppressed as indicated by increased level of reduced glutathione and decreased level of malondialdehyde in comparison with those of drug treated samples. This indicates that TOC, PR and AA may have considerable suppressive action on DIG induced lipid peroxidation. Thus, these antioxidants merit further extensive study to explore their potential in reducing DIG induced toxicity that may be mediated by free radical mediated process.
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Division of Pharmaceutical Chemistry, Semanta Institute of Pharmaceutical Sciences, Mayurbhanj, Orissa.
Roy
K
K
Saha
A
A
Sengupta
C
C
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000009:adverse effects; D004077:Digoxin / Q000009:adverse effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006041:Goats; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D011341:Probucol / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D002316:Cardiotonic Agents; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D004077:Digoxin; D005978:Glutathione; D024502:alpha-Tocopherol; D011341:Probucol; D001205:Ascorbic Acid