title: Crystallization and preliminary X-ray crystallographic analysis of beta-xylosidase from Thermoanaerobacterium saccharolyticum, a thermophilic anaerobe.
abstract: beta-Xylosidases are involved in the breakdown of xylans into xylose and belong to either family 39 or 43 of the glycosyl hydrolases. At present, no structural information is available for any member of these families. beta-Xylosidase from the thermophilic anaerobe Thermoanaerobacterium saccharolyticum, a member of glycosyl hydrolase family 39, has been crystallized at 296 K using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.4 A resolution with synchrotron X-rays and belongs to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 92.75, c = 241.37 A. The asymmetric unit contains two monomers of the recombinant enzyme, giving a corresponding V(M) of 2.21 A(3)Da(-1) and a solvent content of 44.3%.
authors.affiliation: National Research Laboratory of Structural Proteomics, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Jin Kuk
authors.initials: JK
authors.lastname: Yang
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Yoon
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Ahn
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Maris
authors.initials: M
authors.lastname: Laivenieks
authors.forename: Claire
authors.initials: C
authors.lastname: Vieille
authors.forename: J Gregory
authors.initials: JG
authors.lastname: Zeikus
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003013:Clostridium / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D014995:Xylosidases / Q000737:chemistry*
chemical_list: D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase