Highly selective excitation in biomolecular NMR by frequency-switched single-transition cross-polarization.
A new method for selective excitation in biomolecular NMR uses two-fold single-transition cross-polarization between protons and nitrogen-15 or carbon-13 nuclei. Switching the frequencies between the forward and backward transfer steps allows one to select a multiplet pattern that is associated with a single pair of spins in a medium-size protein. The efficiency of the transfer benefits from so-called TROSY line-narrowing effects which arise from interference between relaxation mechanisms.
Ferrage
Fabien
F
Département de Chimie, associé au CNRS, Ecole normale supérieure, 24, rue Lhomond, 75231 Paris Cedex 05, France.
Eykyn
Thomas R
TR
Bodenhausen
Geoffrey
G
D004926:Escherichia coli / Q000201:enzymology; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D016914:Ribonuclease H / Q000737:chemistry
D009587:Nitrogen Isotopes; D016914:Ribonuclease H