[Expression of bFGF in primary human lens epithelial cells].
OBJECTIVE
To study the mechanism of after cataract formation and investigate the expression of basic fibroblast growth factor (bFGF) in primary human lens epithelial cells (PHLECs).


METHODS
Human lens epithelial cells were cultured in vitro, and the expression of bFGF was detected with immunohistochemistry and in situ hybridization.


RESULTS
PHLECs express bFGF in protein and mRNA levels.


CONCLUSIONS
Based on bFGF promoting PHLEC growth and differentiation, the results suggest that bFGF from PHLECs take part in promoting such cell growth and differentiation.
Liu
D
D
Department of Ophthalmology, First Hospital, Beijing Medical University, Beijing 100034.
Wu
J
J
D004847:Epithelial Cells / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007223:Infant; D007231:Infant, Newborn; D007908:Lens, Crystalline / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2