title: Exercise training-induced changes in sensitivity to endothelin-1 and aortic and cerebellum lipid profile in rats.
abstract: The purpose of this work was to study whether exercise training induces changes in the lipid profile of rat aorta and nervous system and in the in vitro intrinsic responsiveness of these tissues to endothel in-1 (ET-1) treatment. The exercise program performed successfully produced the characteristic metabolic alterations of the trained state. Exercise training induced a large and significant increase in the levels of both aortic ethanolamine plasmalogens (PlasEtn) and glucosylceramides. In contrast, a decrease of aortic ceramide and cholesterol levels was evoked by exercise training. ET-1 increased PlasEtn content only in sedentary animals. An exercise-induced increase in cerebellum levels of ceramides and ceramide monohexosides was found. The cerebellum ceramide content was increased by ET-1 more noticeably in sedentary rats than in trained animals. In contrast, cerebral cortex was observed to be largely insensitive to both exercise training and ET-1 treatment. It was concluded that exercise training (i) induces changes in both vascular and cerebellar lipid profiles, the former being much more pronounced than the latter, and (ii) diminishes the aortic and cerebellar sensitivity to ET-1 action.
authors.affiliation: Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Latorre
authors.forename: Maria
authors.initials: M
authors.lastname: Morán
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Aragonés
authors.forename: Ana
authors.initials: A
authors.lastname: Saborido
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Fernández
authors.forename: Jerónimo
authors.initials: J
authors.lastname: Delgado
authors.forename: R Edgardo
authors.initials: RE
authors.lastname: Catalán
authors.forename: Alicia
authors.initials: A
authors.lastname: Megías
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004351:Drug Resistance; D019332:Endothelin-1 / Q000494:pharmacology*; D005963:Glucosylceramides / Q000378:metabolism; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010955:Plasmalogens / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D002518:Ceramides; D019332:Endothelin-1; D005963:Glucosylceramides; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D002784:Cholesterol; D002950:Citrate (si)-Synthase