Primary culture of porcine pancreatic acinar cells.
OBJECTIVE
To develop a method for the primary culture of porcine pancreatic acinar cells.


METHODS
Dispersed pancreatic acinar cells available utilizing RPMI-1640 medium containing collagenase III. After purification, the isolated acinar cells were cultured in RPMI-1640 medium with the addition of 2.5% fetal bovine serum.


METHODS
The morphological characteristics of acinar cells were described. (3)H-thymidine incorporation of acinar cells and the activity of amylase or lipase were determined during the culture process.


RESULTS
There were no remarkable morphological changes in the pancreatic acinar cells during the 20 days culture. The acini showed a tendency to gather but did not attach to the walls of the culture disks. A good (3)H-thymidine incorporation of acinar cells in the primary culture was maintained. The secretion of amylase or lipase from the acini decreased with the length of time of the culture.


CONCLUSIONS
The primary culture of acinar cells from a porcine pancreas which was carried out in this study maintained the normal morphology of the acinar cells and their ability to grow but not their secretion of amylase or lipase. The method would benefit by the further experiments on acini of porcine pancreas.
Zhao
X
X
Department of Gastroenterology, The First Hospital, Chongqing University of Medical Sciences. Chongqing, P. R. China.
Han
J
J
Tang
C
C
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D010189:Pancreatic Juice / Q000378:metabolism; D013552:Swine