[The dynamic changes of postburn complement activation and its effects on the secretory function of macrophages in severely burned mice].
OBJECTIVE
To study the dynamic changes of postburn complement activation and its effects on the secretory function of macrophages in severely burned mice, so as to help explore the mechanism of postburn immunological disturbance.


METHODS
The mice inflicted with 18% TBSA III degree scalding were employed as the model and randomly divided into normal control, decomplementizing control, scalding and decomplementizing scalding groups. The chemotactic activity of plasma, the production of nitride oxide (NO) and tumor necrosis factor alpha (TNFalpha) by peritoneal macrophages (MPhi) cultured by plasma from every groups were examined.


RESULTS
The chemotactic activity of plasma complements increased quickly within 30 min. postburn, reached the summit level at 6 postburn hour (PBH) and declined since 12 PBH. Plasma from injured mice could induce peritoneal MPhis to produce more NO and TNFalpha.


CONCLUSIONS
Complements were immediately activated postburn. The MPhis were activated by active complements. As a result, immunological active products increased significantly.
Hu
Y
Y
Institute of Burn Research, Southwestern Hospital, The Third Military Meaical University, Chongqing 400038, P.R. China.
Peng
D
D
Huang
W
W
D000818:Animals; D002056:Burns / Q000276:immunology*; D002478:Cells, Cultured; D003167:Complement Activation; D005260:Female; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide