[Effects of holothuria glycosaminoglycan on the expression of tissue factor and thrombomodulin in stimulated endothelial cells].
OBJECTIVE
To study the mechanism of antithrombotic effects of holothuria glycosaminoglycan (GAG).


METHODS
Endothelial cells from human umbilical vein which were pre-induced with 1 mg/L lipopolysaccharide, were treated by GAG (1 mg/L, 5 mg/L, and 10 mg/L, respectively) and 5 mg/L heparin as a control for 6 hours. Procoagulant activity (PCA), the expressions of tissue factor (TF) antigen and thrombomodulin (TM) antigen and their mRNA transcriptions were investigated.


RESULTS
GAG could down-regulate the expression of TF antigen and mRNA, up-regulate the expression of TM antigen and mRNA, down-regulate the PCA of stimulated endothelial cells.


CONCLUSIONS
Down-regulating TF expression and up-regulating TM expression of stimulated endothelial cells may be one of the mechanisms of GAG antithrombosis.
Li
Z
Z
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Wang
X
X
Wang
H
H
Guo
W
W
Liu
Y
Y
Qu
B
B
Guo
X
X
Chu
H
H
D000818:Animals; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D012616:Sea Cucumbers / Q000737:chemistry*; D018180:Thrombomodulin / Q000032:analysis* / Q000235:genetics*; D013925:Thromboplastin / Q000032:analysis* / Q000235:genetics
D006025:Glycosaminoglycans; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D018180:Thrombomodulin; D006493:Heparin; D013925:Thromboplastin