Essential oil composition of sachalinmint from Norway detected by solid-phase microextraction and gas chromatography-mass spectrometry analysis.
The essential oil of leaves and flowers of sachalinmint [Mentha sachalinensis (Briq.) Kudô] grown in Norway (Trondheim) has been studied by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry analysis (GC-MS). The essential oil content increased linearly in acropetal direction from 1.08% (0-20 cm plant height) to 1.75% (60-80 cm; young leaves and flowers). The steam-distilled samples showed a minor complex matrix with a very high menthol and a much lower menthone content (87.89 and 4.05%, respectively). From testing of HS-SPME unequilibrated exposure times ranging from 10 s to 5 min, an extraction time of 30 s was found to be sufficient to detect both low- and high-eluting compounds. Comparison of HS-SPME and steam-distilled samples established that the same tendencies of increasing menthol/menthone content in the basipetal/acropetal direction could be detected by both analysis methods. With regard to the extraction efficiency, HS-SPME gave additional detailed information about less important terpenic compounds.
Rohloff
Jens
J
The Plant Biocentre, Department of Botany, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway. jens.rohloff@chembio.ntnu.no
D008401:Gas Chromatography-Mass Spectrometry; D027541:Mentha / Q000737:chemistry*; D008610:Menthol / Q000032:analysis; D009664:Norway; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D018514:Plant Structures / Q000737:chemistry; D013227:Steam; D013729:Terpenes / Q000032:analysis
D010938:Plant Oils; D013227:Steam; D013729:Terpenes; D008610:Menthol; C019466:menthone