Depletion of human NK and CD8 cells prior to in vitro H1N1 flu vaccine stimulation increases the number of gamma interferon-secreting cells compared to the initial undepleted population in an ELISPOT assay.
In order to study the respective roles of CD4, CD8, and CD56 (NK) cells in gamma interferon (IFN-gamma) production after in vitro stimulation with flu vaccine in a healthy adult human population, we depleted these cellular subtypes before stimulation with antigen (inactivated split vaccine, A/Texas H1N1, or A/Sydney H3N2). We observed that while CD4 cells were required for IFN-gamma secretion in both conditions in vitro, CD56 (NK) cells and, to a lesser extent, CD8 cells had a negative effect on such synthesis upon H1N1 stimulation, as judged by an increased number of spots compared to the initial undepleted population. This regulation of IFN-gamma secretion was associated with an increase in ICAM-1 expression, in particular on T and B cells. This study points out the importance of evaluating in vitro immune responses on a whole-cell population in addition to isolated subtypes if one needs to address potential cellular interactions occurring in vivo in some situations (H1N1 stimulation in the present case). Such cross-regulations occur even in vitro during the antigenic stimulation step.
Dercamp
Christophe
C
Research Department, Aventis Pasteur, 69280 Marcy l'Etoile, France.
Sanchez
Violette
V
Barrier
Julie
J
Trannoy
Emanuelle
E
Guy
Bruno
B
D000328:Adult; D000956:Antigens, Viral / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002450:Cell Communication / Q000276:immunology; D002469:Cell Separation; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007252:Influenza Vaccines / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000166:cytology*
D000956:Antigens, Viral; D007252:Influenza Vaccines; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma