title: Susceptibility of Helicobacter pylori and its urease activity to the peroxidase-hydrogen peroxide-thiocyanate antimicrobial system.
abstract: The susceptibility of Helicobacter pylori to the antimicrobial system involving lactoperoxidase, hydrogen peroxide and thiocyanate was investigated. The inhibitory effect of the system on the urease activity of H. pylori, which plays a role in its colonisation of the stomach, was also investigated. Twelve H. pylori strains examined, including 10 clinical isolates, were all inhibited by the peroxidase system in brain-heart infusion broth supplemented with fetal calf serum, but to different extents. The killing effect was observed within 3 h. Although bacterial viability recovered afterwards, there was still a clear difference between cultures incubated in the presence of the complete system and control cultures incubated in the absence of lactoperoxidase, after incubation for 24 h. The urease activity and viability of H. pylori were both inactivated by this system in phosphate buffer. These effects were dependent on the concentrations of both lactoperoxidase and hydrogen peroxide and were abolished by the addition of cysteine. Furthermore, these effects were observed when bovine lactoperoxidase was replaced by recombinant human lactoperoxidase or native or recombinant human myeloperoxidase. The peroxidase system found in saliva and milk may contribute to the host defence against H. pylori infection and inhibition of transmission via the oral route.
authors.forename: K
authors.initials: K
authors.lastname: Shin
authors.forename: K
authors.initials: K
authors.lastname: Yamauchi
authors.forename: S
authors.initials: S
authors.lastname: Teraguchi
authors.forename: H
authors.initials: H
authors.lastname: Hayasawa
authors.affiliation: Nutritional Science Laboratory, Morinaga Milk Industry Co. Ltd, 5-1-83 Higashihara, Zama, Kanagawa 228-8583 and *Third Department of Internal Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Imoto
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002021:Buffers; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007784:Lactoperoxidase / Q000494:pharmacology; D009195:Peroxidase / Q000494:pharmacology; D012463:Saliva / Q000502:physiology; D013861:Thiocyanates / Q000494:pharmacology*; D013997:Time Factors; D014510:Urease / Q000037:antagonists & inhibitors*
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D004791:Enzyme Inhibitors; D013861:Thiocyanates; C014607:hypothiocyanite ion; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D009195:Peroxidase; D014510:Urease; C031760:thiocyanate