Human oviductin mRNA expression is not maintained in oviduct mucosal cell culture.
OBJECTIVE
To determine whether oviduct mucosal cell culture supports the continued production of oviductin, a putative embryotrophic protein.


METHODS
Semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR) analysis of oviductin messenger RNA (mRNA) expression after oviduct mucosal cell culture.


METHODS
University-based obstetrics and gynecology department.


METHODS
Ten women undergoing laparoscopy for tubal sterilization or hysterectomy for uterine fibroids.


METHODS
The mucosal layer was isolated from the oviduct tissue and subjected to routine culture conditions; semiquantitative RT-PCR was performed.


METHODS
The relationship between duration of cell culture and expression of oviductin mRNA.


RESULTS
There was a significant reduction in oviductin mRNA expression after 3 days in culture, with a complete loss after 6 days in 70% of the samples and after 12 days in the remaining 30%. CONLCUSION(S): This is the first study to investigate whether oviductin mRNA continues to be expressed in cultured human oviduct mucosal cells. Our results suggest that oviduct mucosal cells lose their ability to produce oviductin after short-term culture. This method of culture does not appear to be appropriate for a coculture system reliant upon oviductal secretion of oviductin.
Briton-Jones
Christine
C
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. chrisbj@cuhk.edu.hk
Lok
Ingrid Hung
IH
Yuen
Pong Mo
PM
Chiu
Tony Tak Yu
TT
Cheung
Lai Ping
LP
Haines
Christopher
C
D046508:Culture Techniques; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005498:Follicular Phase / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008183:Luteal Phase / Q000378:metabolism; D009092:Mucous Membrane / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics; D018709:Statistics, Nonparametric
D018076:DNA, Complementary; D012333:RNA, Messenger; D012697:Serine Endopeptidases; C074599:oviductin