title: Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants.
abstract: This study explored the possibility of using non-viral, plant-based gene sequences to create strong and constitutive expression vectors. Replacement histone H3 genes are highly and constitutively expressed in all plants. Sequences of the cloned alfalfa histone H3.2 gene MsH3gl were tested. Constructs of the beta-glucuronidase (GUS) reporter gene were produced with H3.2 gene promoter and intron sequences. Their efficiency was compared with that of the commonly used strong 35S cauliflower mosaic virus promoter in transgenic tobacco plants. Combination of the H3.2 promoter and intron produced significantly higher GUS expression than the strong viral 35S promoter. Histochemical GUS analysis revealed a constitutive pattern of expression. Thus, alfalfa replacement H3 gene sequences can be used instead of viral promoters to drive heterologous gene expression in plants, avoiding perceived risks of viral sequences.
authors.affiliation: Institute of Plant Biology, Biological Research Center Hungarian Academy of Sciences, Szeged.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Kelemen
authors.forename: Antal
authors.initials: A
authors.lastname: Mai
authors.forename: Tamás
authors.initials: T
authors.lastname: Kapros
authors.forename: Attila
authors.initials: A
authors.lastname: Fehér
authors.forename: János
authors.initials: J
authors.lastname: Györgyey
authors.forename: Jakob H
authors.initials: JH
authors.lastname: Waterborg
authors.forename: Dénes
authors.initials: D
authors.lastname: Dudits
mesh_terms: D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics; D017931:DNA Primers; D017930:Genes, Reporter; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics; D006657:Histones / Q000235:genetics*; D007438:Introns; D000455:Medicago sativa / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D017931:DNA Primers; D006657:Histones; D014764:Viral Proteins; D005966:Glucuronidase