title: Crystallization and preliminary X-ray characterization of the acylphosphatase-like domain from the Escherichia coli hydrogenase maturation factor HypF.
abstract: Maturation of prokaryotic hydrogenase involves several protein factors, among which is the accessory protein HypF, which hosts the consensus sequence of acylphosphatases and a sequence motif common to proteins catalyzing O-carbamoylations. The specific functions of HypF are largely unknown, although it has been observed that CN(-) and CO ligands at the hydrogenase Ni,Fe active centre originate from carbamoylphosphate. The HypF N-terminal domain (91 residues, acylphosphatase-like domain) has been crystallized in two different crystal forms belonging to the orthorhombic P2(1)2(1)2(1) space group (unit-cell parameters a = 35.5, b = 59.8, c = 87.6 A) and to the rhombohedral space group R32 (unit-cell parameters a = b = 58.1, c = 155.6 A in the hexagonal setting).
authors.affiliation: Advanced Biotechnology Center--IST, Largo Rosanna Benzi 10, 16132 Genoa, Italy.
authors.forename: Camillo
authors.initials: C
authors.lastname: Rosano
authors.forename: Simone
authors.initials: S
authors.lastname: Zuccotti
authors.forename: Massimo
authors.initials: M
authors.lastname: Stefani
authors.forename: Monica
authors.initials: M
authors.lastname: Bucciantini
authors.forename: Giampietro
authors.initials: G
authors.lastname: Ramponi
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000737:chemistry; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D000097024:Acylphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D001426:Bacterial Proteins; D000097024:Acylphosphatase; C108878:HypF protein, Bacteria