Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism.
S. latifolia is a dioecious plant with morphologically distinct sex chromosomes. To genetically map the sex determination loci on the male-specific Y chromosome, we identified X-ray-induced sex determination mutants that had lost male traits. We used male-specific AFLP markers to characterize the extent of deletions in the Y chromosomes of the mutants. We then compared overlapping deletions to predict the order of the AFLP markers and to locate the mutated sex-determining genes. We found three regions on the Y chromosome where frequent deletions were significantly associated with loss of male traits. One was associated with hermaphroditic mutants. A second was associated with asexual mutants that lack genes needed for early stamen development and a third was associated with asexual mutants that lack genes for late stages of stamen development. Our observations confirmed a classical genetic prediction that S. latifolia has three dispersed male-determining loci on the Y chromosome, one for carpel suppression, one for early stamen development, and another for late stamen development. This AFLP map provides a framework for locating genes on the Y chromosome and for characterizing deletions on the Y chromosomes of potentially interesting mutants.
Lebel-Hardenack
Sabine
S
Department of Biology and Curriculum in Molecular Biology and Genetics, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Hauser
Elizabeth
E
Law
Teresa F
TF
Schmid
Jurg
J
Grant
Sarah R
SR
D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017353:Gene Deletion; D005819:Genetic Markers; D009154:Mutation / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D019849:Sex Determination Processes; D029751:Silene / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
D005819:Genetic Markers