The A17L gene product of vaccinia virus is exposed on the surface of IMV.
The p21 membrane protein of vaccinia virus (VV), encoded by the A17L gene, has been reported to localize on the inner of the two membranes of the intracellular mature virus (IMV). It has also been shown that p21 acts as a membrane anchor for the externally located fusion protein p14 (A27L gene). Since p14 is located on the surface of IMVs, it is hard to envision that p21 should be located only on the inner membrane. Our results from (i) immunoelectron microscopy, (ii) biotinylation, and (iii) protease treatment of purified IMVs showed that the N-terminus of p21 is exposed on the surface of virus particles, while the C-terminus is embedded in the membrane. Mono-specific antibodies to the N-terminus of p21 neutralize infection of VV while antibodies to the C-terminal domain do not. We suggest that p21 molecules are located both in the inner and in the outer membrane of IMV.
Wallengren
K
K
Department of Biosciences at Novum, Karolinska Institute, S-141 57 Huddinge, Sweden.
Risco
C
C
Krijnse-Locker
J
J
Esteban
M
M
Rodriguez
D
D
D000818:Animals; D001665:Binding Sites; D001710:Biotin; D002460:Cell Line; D002522:Chlorocebus aethiops; D008565:Membrane Proteins; D016253:Microscopy, Immunoelectron / Q000379:methods; D009500:Neutralization Tests; D014357:Trypsin; D014616:Vaccinia virus / Q000737:chemistry* / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000276:immunology
C081257:A17L protein, Vaccinia virus; D008565:Membrane Proteins; D014759:Viral Envelope Proteins; D001710:Biotin; D014357:Trypsin