Effects of GroESL coexpression on the folding of nicotinoprotein formaldehyde dismutase from Pseudomonas putida F61.
The overexpression of fdm, which encodes the formaldehyde dismutase from Pseudomonas putida F61, resulted in the formation of inclusion bodies made up of aggregated enzyme, leaving little activity in the soluble fraction of the transformant cells. On the other hand, coexpression of groESL along with fdm facilitated in vivo solubilization of the enzyme protein in its active form. When coexpressed with groESL, formaldehyde dismutase purified from E. coli had the same crystalline form (i.e., a regular octahedron) as the native enzyme, and like the native enzyme, it bound 1 mol of NAD(H) and 2 mol of zinc in each subunit.
Yanase
Hideshi
H
Department of Biotechnology, Faculty of Engineering, Tottori University, Japan. yanase@bio.tottori-u.ac.jp
Moriya
Keishi
K
Mukai
Norihiko
N
Kawata
Yasushi
Y
Okamoto
Kenji
K
Kato
Nobuo
N
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002455:Cell Division; D018833:Chaperonins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D015870:Gene Expression; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; C097970:GroESL protein, Bacteria; D000429:Alcohol Oxidoreductases; C048530:formaldehyde dismutase; D018833:Chaperonins