Inhibition of copper amine oxidases by pyridine-derived aldoximes and ketoximes.
The reactions of pea diamine oxidase (PSAO) and 2-phenylethylamine oxidase from Arthrobacter globiformis (AGAO) with pyridine-derived oximes were studied. Pyridine carbaldoximes and alkyl pyridyl ketoximes act as strong non-competitive inhibitors of the enzymes. The inhibition constants K(i) of these compounds vary between 10(-4) and 10(-5) M, for AGAO and some of the studied oximes were found even micromolar K(i) values. The presence of pyridine moiety in the studied compounds has remarkable influence on the inhibition potency. Elementary oximes lacking the heterocyclic ring, i.e., aliphatic (acetone oxime), alicyclic (cyclohexanone oxime) and aromatic (benzaldoxime), are considerably weaker non-competitive inhibitors (K(i) similar to 10(-3) or 10(-2) M). The position of the pyridine ring substitution by -C(R)=NOH group does not play a significant role for the inhibition potency of the studied oxime compounds. If the pyridine nitrogen is quaternised (in hydroxyiminomethyl-1-methylpyridinium iodides), the compound looses its inhibitory properties. Extended length of alkyl substituents on the ketoxime group of alkyl pyridyl ketoximes increases the K(i) value. The enzyme-bound copper represents one of possible target sites for pyridine-derived oxime inhibitors. The addition of an alkyl pyridyl ketoxime or a pyridine carbaldoxime to a native PSAO sample perturbs the absorption spectrum of the enzyme (by an absorption increase in the region 300-400 nm) that is not observed in the spectrum of reacted PSAO apoenzyme. However, an additional formation of hydrogen bonds with amino acid side-chains at the active site should be considered, namely for 3- and 4-substituted pyridine derivatives.
Mlícková
K
K
Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, 783 71 Olomouc, Czech Republic.
Sebela
M
M
Cibulka
R
R
Frébort
I
I
Pec
P
P
Liska
F
F
Tanizawa
K
K
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors*; D001173:Arthrobacter / Q000201:enzymology*; D007700:Kinetics; D010091:Oximes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018532:Pisum sativum / Q000201:enzymology*; D013329:Structure-Activity Relationship
D010091:Oximes; D006631:Amine Oxidase (Copper-Containing); C023728:acetaldehyde oxime