title: Species-level identification of orthopoxviruses with an oligonucleotide microchip.
abstract: A method for species-specific detection of orthopoxviruses pathogenic for humans and animals is described. The method is based on hybridization of a fluorescently labeled amplified DNA specimen with the oligonucleotide DNA probes immobilized on a microchip (MAGIChip). The probes identify species-specific sites within the crmB gene encoding the viral analogue of tumor necrosis factor receptor, one of the most important determinants of pathogenicity in this genus of viruses. The diagnostic procedure takes 6 h and does not require any sophisticated equipment (a portable fluorescence reader can be used).
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Lapa
authors.forename: Maxim
authors.initials: M
authors.lastname: Mikheev
authors.forename: Sergei
authors.initials: S
authors.lastname: Shchelkunov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mikhailovich
authors.forename: Alexander
authors.initials: A
authors.lastname: Sobolev
authors.forename: Vladimir
authors.initials: V
authors.lastname: Blinov
authors.forename: Igor
authors.initials: I
authors.lastname: Babkin
authors.forename: Alexander
authors.initials: A
authors.lastname: Guskov
authors.forename: Elena
authors.initials: E
authors.lastname: Sokunova
authors.forename: Alexander
authors.initials: A
authors.lastname: Zasedatelev
authors.forename: Lev
authors.initials: L
authors.lastname: Sandakhchiev
authors.forename: Andrei
authors.initials: A
authors.lastname: Mirzabekov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D015342:DNA Probes; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D018155:Orthopoxvirus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D015342:DNA Probes; D018124:Receptors, Tumor Necrosis Factor; D014764:Viral Proteins; C089458:crmB protein, Orthopoxvirus