A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer.
Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope.
Sellrie
F
F
Institute of Biochemistry and Biology, Biotechnology, Potsdam University, Karl-Liebknecht-Str. 24-25, 14476 Golm, Germany.
Schenk
J A
JA
Behrsing
O
O
Böttger
V
V
Micheel
B
B
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002417:Cattle; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D006241:Haptens / Q000032:analysis*; D006735:Horseradish Peroxidase; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D006241:Haptens; D009842:Oligopeptides; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate