Neither the density nor function of striatal dopamine transporters were influenced by chronic n-3 polyunsaturated fatty acid deficiency in rodents.
We hypothesized that the chronic dietary deficiency of n-3 polyunsaturated fatty acids (n-3 PUFAs) might affect the density and/or function of dopamine transporters (DAT), which have a major role in regulating the synaptic level of dopamine. This hypothesis was tested by investigating DAT in the striatum using three complementary methods in control and deficient rats. The density of DAT was determined by quantitative autoradiography using [(125)I]PE2I, a specific ligand of this transporter. Functional investigations were performed (i) in vitro by measuring [(3)H]dopamine uptake on synaptosomes, and (ii) in vivo using intracerebral microdialysis. The results demonstrated that neither the density nor the function of DAT were influenced by n-3 PUFA deficiency in the striatum. This suggests lower sensitivity to n-3 PUFA deficiency in the striatum than that previously observed in the frontal cortex.
Kodas
Ercem
E
INSERM U316, Laboratoire de Biophysique Médicale et Pharmaceutique, Université François Rabelais, UFR des Sciences Pharmaceutiques, 31 Avenue Monge, 37200 Tours, France. kodas@univ-tours.fr
Page
Guylène
G
Zimmer
Luc
L
Vancassel
Sylvie
S
Guilloteau
Denis
D
Durand
Georges
G
Chalon
Sylvie
S
D000818:Animals; D004041:Dietary Fats / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D050483:Dopamine Plasma Membrane Transport Proteins; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D005260:Female; D005526:Food, Formulated / Q000009:adverse effects*; D007457:Iodine Radioisotopes / Q000378:metabolism / Q000494:pharmacology; D008055:Lipids / Q000172:deficiency*; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins / Q000378:metabolism*; D017072:Neostriatum / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D017729:Presynaptic Terminals / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014316:Tritium / Q000378:metabolism
D004041:Dietary Fats; D050483:Dopamine Plasma Membrane Transport Proteins; D015525:Fatty Acids, Omega-3; D007457:Iodine Radioisotopes; D008055:Lipids; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493216:Slc6a3 protein, rat; D014316:Tritium; D004298:Dopamine