title: GATA-1 binding sites mapped in the beta-globin locus by using mammalian chIp-chip analysis.
abstract: The expression of the beta-like globin genes is intricately regulated by a series of both general and tissue-restricted transcription factors. The hemapoietic lineage-specific transcription factor GATA-1 is important for erythroid differentiation and has been implicated in regulating the expression of the erythroid-specific genes including the genes of the beta-globin locus. In the human erythroleukemic K562 cell line, only one DNA region has been identified previously as a putative site of GATA-1 interaction by in vivo footprinting studies. We mapped GATA-1 binding throughout the beta-globin locus by using chIp-chip analysis of K562 cells. We found that GATA-1 binds in a region encompassing the HS2 core element, as was previously identified, and an additional region of GATA-1 binding upstream of the gammaG gene. This approach will be of general utility for mapping transcription factor binding sites within the beta-globin locus and throughout the genome.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Horak
authors.forename: Milind C
authors.initials: MC
authors.lastname: Mahajan
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Luscombe
authors.forename: Mark
authors.initials: M
authors.lastname: Gerstein
authors.forename: Sherman M
authors.initials: SM
authors.lastname: Weissman
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002843:Chromatin; D002874:Chromosome Mapping; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D008322:Mammals; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D011233:Precipitin Tests; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; C494688:GATA1 protein, human; D014157:Transcription Factors; D005914:Globins