[Evaluation of p53, p21waf1, and PCNA in the diagnosis on lung cancer].
OBJECTIVE
To evaluate the diagnostic value of p53, p21waf1 and PCNA on diagnosis of lung cancer.


METHODS
One hundred and fourteen lung cancer patients and 89 cases with benign pulmonary diseases were studied on the expression of p53, p21waf1 and PCNA by immunohistochemical staining. The differences of p53, p21waf1 and PCNA expression status between these two groups were compared and the diagnostic value of these three markers was evaluated.


RESULTS
(1) The positive rates of p53, p21waf1 and PCNA expression in lung cancer were 47.37%, 75.44% and 80.70%, all significantly higher than that in benign pulmonary diseases (P < 0.001), with odds ratio 39.15, 5.75, 6.76 respectively. (2) The positive likelihood ratio of p53 was 21.08, higher than that of p21waf1 and PCNA. Specificity and Youden index were also higher than those of p21waf1 and PCNA, but the sensitivity of p53 was low. (3) The sensitivity and specificity of p53 and PCNA parallel test were 89.47% and 60.67% respectively.


CONCLUSIONS
(1) The expression of p53, p21waf1 and PCNA in lung cancer cases were all upregulated. These three markers all seemed to have strong links with lung cancer. (2) p53 was shown a good marker for lung cancer diagnosis. (3) The diagnostic value of p53 and PCNA parallel test was rather high.
Ding
X
X
Department of Respiratory Medicine, the First Affiliated Hospital of Hubei Medical University, Wuhan 430060, China.
Yang
J
J
Hu
S
S
Wu
X
X
Li
Q
Q
D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008171:Lung Diseases / Q000378:metabolism; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D016017:Odds Ratio; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012680:Sensitivity and Specificity; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D014408:Biomarkers, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53