title: Wax ester production from n-alkanes by Acinetobacter sp. strain M-1: ultrastructure of cellular inclusions and role of acyl coenzyme A reductase.
abstract: Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Ishige
authors.forename: Akio
authors.initials: A
authors.lastname: Tani
authors.forename: Keiji
authors.initials: K
authors.lastname: Takabe
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D017353:Gene Deletion; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D014885:Waxes / Q000378:metabolism*
chemical_list: D000473:Alkanes; D004952:Esters; D014885:Waxes; D000445:Aldehyde Oxidoreductases; C021351:hexadecanal dehydrogenase (acylating)