title: Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy.
abstract: An enzyme-linked immunosorbent assay (Elisa), using as antigen canine coronavirus-infected CrFK cell supernatant, was developed to detect antibodies against canine coronavirus (CCoV). Out of a total of 109 dog serum samples, 80 which were positive by routine virus neutralisation test were also Elisa positive. Seventeen samples which were negative by the virus neutralisation test, were positive by Elisa and by the confirmatory Western blotting test. The Elisa was substantially more sensitive than the virus neutralisation test in detecting antibodies to CCoV and may be used as an alternative technique to virus neutralisation.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010, Valenzano, Bari, Italy. a.pratelli@veterinaria.uniba.it
authors.forename: Annamaria
authors.initials: A
authors.lastname: Pratelli
authors.forename: Gabriella
authors.initials: G
authors.lastname: Elia
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Alessandra
authors.initials: A
authors.lastname: Palmieri
authors.forename: Francesco
authors.initials: F
authors.lastname: Cirone
authors.forename: Antonella
authors.initials: A
authors.lastname: Tinelli
authors.forename: Marialaura
authors.initials: M
authors.lastname: Corrente
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D018352:Coronavirus Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017939:Coronavirus, Canine / Q000276:immunology / Q000302:isolation & purification*; D004283:Dog Diseases / Q000453:epidemiology / Q000276:immunology / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007558:Italy / Q000453:epidemiology; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral