title: The neutrophil-activating protein (HP-NAP) of Helicobacter pylori is a potent stimulant of mast cells.
abstract: Infection by Helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa. A neutrophil-activating protein (HP-NAP) has been identified in H.pylori, and its role in infection and immune response is currently under investigation. Here, we show that HP-NAP induces beta-hexosaminidase release and interleukin-6 production in peritoneal mast cells, two actions which are completely inhibited by pertussis toxin. We also show that in polarized epithelial cell monolayers HP-NAP translocates from the apical to the basolateral domain, where mast cells are located. These findings characterize HP-NAP as an inflammatory factor of H.pylori that is effective from the beginning of the inflammatory cascade.
authors.affiliation: Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, Padova, Italy.
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Montemurro
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishioka
authors.forename: William G
authors.initials: WG
authors.lastname: Dundon
authors.forename: Marina
authors.initials: M
authors.lastname: de Bernard
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Del Giudice
authors.forename: Rino
authors.initials: R
authors.lastname: Rappuoli
authors.forename: Cesare
authors.initials: C
authors.lastname: Montecucco
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D016764:Cell Polarity; D002630:Chemotactic Factors / Q000494:pharmacology / Q000502:physiology*; D003110:Colonic Neoplasms / Q000473:pathology; D003594:Cytoplasmic Granules / Q000378:metabolism; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D016480:Helicobacter pylori / Q000502:physiology*; D006636:Histamine Release / Q000187:drug effects; D007249:Inflammation; D016209:Interleukin-8 / Q000096:biosynthesis; D007476:Ionophores / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D010529:Peritoneal Cavity / Q000166:cytology; D037342:Pertussis Toxin; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002630:Chemotactic Factors; D016209:Interleukin-8; D007476:Ionophores; D010566:Virulence Factors, Bordetella; C092412:neutrophil-activating protein A, Helicobacter pylori; D000001:Calcimycin; D037342:Pertussis Toxin; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium