title: [Humoral response to the injection of acellular Pertussis vaccine].
abstract: The humoral response of mice and rabbits to the injection of whole-cell pertussis vaccine (PV) and acellular pertussis vaccine (APV), developed at the Mechnikov Research Institute for Vaccines and Sera (Russian Acad. Med. Sci.) in Moscow, was studied. In the sera of immunized animals antibodies to the antigenic complex were determined in the direct hemagglutination (DHA) test, specific antibodies to filamentous hemagglutinin (FHA) and pertussis toxin (PT)--in the enzyme immunoassay (EIA) and antibodies neutralizing PT in a cytopathogenic dose (CPD)--in neutralization test on Chinese hamster ovary (CHO) cells. In mice and rabbits immunized with APV the antibody titers determined in the DHA test were higher than those in the animals immunized with PV. Specific antibodies titers to FHA and PT in the sera of rabbits immunized with APV were also higher than those in the sera of rabbits immunized with PV. High dilutions of sera taken from the animals immunized with APC neutralized 4-16 doses of PT in the neutralization test on CHO cells. The most important result of this study was the detection of a more pronounced immune response in the animals immunized with APV in comparison with that induced by PV according to the results obtained in EIA and in the test of PT CPD neutralization on CHO cells.
authors.affiliation: Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.
authors.forename: N S
authors.initials: NS
authors.lastname: Zakharova
authors.forename: T N
authors.initials: TN
authors.lastname: Remova
authors.forename: M V
authors.initials: MV
authors.lastname: Britsina
authors.forename: E I
authors.initials: EI
authors.lastname: Shmeleva
authors.forename: N U
authors.initials: NU
authors.lastname: Mertsalova
authors.forename: E M
authors.initials: EM
authors.lastname: Zaĭtsev
authors.forename: M N
authors.initials: MN
authors.lastname: Ozeretskovskaia
authors.forename: I G
authors.initials: IG
authors.lastname: Bazhanova
authors.forename: E V
authors.initials: EV
authors.lastname: Ermolova
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D001886:Bordetella pertussis / Q000276:immunology*; D016466:CHO Cells; D006224:Cricetinae; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000276:immunology; D007114:Immunization; D007124:Immunoenzyme Techniques; D051379:Mice; D009500:Neutralization Tests; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D014611:Vaccination; D022282:Vaccines, Acellular / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000517:prevention & control
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D015164:Vaccines, Inactivated; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D037342:Pertussis Toxin