Isolation of lilin, a novel arginine- and glutamate-rich protein with potent antifungal and mitogenic activities from lily bulbs.
From the dried bulbs of the lily (Lilium brownii), a protein with strong antifungal and mitogenic activities was isolated. It also exhibited an inhibitory action on the activity of HIV-1 reverse transcriptase. The protein was single-chained and possessed a molecular weight of 14.4 kDa and an N-terminal sequence distinct from chitinases and antimicrobial proteins of garlic, leek and onion which belong to a family closely related to lily. However, there was a small degree of resemblance to cyclophilins and a considerable extent of identity to the 6.5 kDa arginine/glutamate-rich polypeptide from Luffa cylindrica seeds. A nearly homogeneous preparation was obtained after the extract was fractionated on DEAE-cellulose and Affi-gel Blue gel since subsequent chromatography on Mono S and Superdex 75 both yielded a single peak.
Wang
Hexiang
H
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing.
Ng
Tzi Bun
TB
D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002846:Chromatography, Affinity; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005658:Fungi / Q000187:drug effects / Q000254:growth & development; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006384:Hemagglutination / Q000187:drug effects; D027762:Lilium / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D008934:Mitogens / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D018749:RNA, Plant / Q000037:antagonists & inhibitors / Q000187:drug effects; D012343:RNA, Transfer / Q000187:drug effects
D000935:Antifungal Agents; D004365:Drugs, Chinese Herbal; D008934:Mitogens; D010940:Plant Proteins; D018749:RNA, Plant; C448822:lilin protein, Lilium brownii; D012343:RNA, Transfer; D054303:HIV Reverse Transcriptase