[Assessment of six drug sensitivities in established cell lines of SO-Rb50 and SO-Rb70].
OBJECTIVE
With retinoblastoma (Rb) cell lines cultured in vitro to screen chemotherapeutic drugs that are effective on Rb.


METHODS
Sensitivity experiments were performed with the 3, -4, 5 Dimethyliazol-2, 5 diphenyl tetrazolium bromide (MTT) assay for the drugs: dactinomycin (DACT), vincristine (VCR), VM-26, daunorubicin (DNR), DDP, bleomycin (BLMA5) against the tumor cell lines of SO-Rb50 and SO-Rb70.


RESULTS
The IC(50) values (microg/ml) in order at 48 hours of the above pharmaceuticals against SO-Rb50 were respectively 0.0004, 0.0016, 0.0389, 0.047, 0.29, 0.44, and those at 72 hours were 0.00025, 0.00081, 0.0151, 0.0192, 0.097, 0.11. And the IC(50) values (microg/ml) in order at 48 hours of the above pharmaceuticals against SO-Rb70 were respectively 0.00065, 0.00149, 0.0282, 0.043, 0.37, 0.215, and those at 72 hours were 0.00042, 0.00082, 0.0146, 0.0176, 0.035, 0.084.


CONCLUSIONS
The tumor cell lines of SO-Rb50 and SO-Rb70 were sensitive to the above six pharmaceuticals. By their IC(50) values, the sensitivity order to the drugs, from higher to lower, was DACT, VCR, VM-26, DNR, DDP, BLMA5.
Zhang
P
P
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060.
Feng
G
G
Yi
Y
Y
Wu
Z
Z
Li
Y
Y
Zheng
J
J
D000970:Antineoplastic Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D019572:Retinal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D012175:Retinoblastoma / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents