Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex.
OBJECTIVE
To isolate the G4 and G1 molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs.


METHODS
Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and G1 molecular forms of AChE.


RESULTS
After size exclusion chromatography, two AChE active peaks were gotten. The putative molecular weights of the two highest AChE active fractions were around Mr 239 000 and Mr 68 000 respectively, which represented G4 and G1 AChE isoforms. The sedimentation coefficients of the Mr 239 000 and Mr 68 000 fractions were 10 S and 4 S, which also assigned to G4 and G1 AChE isoforms.


CONCLUSIONS
The G4 and G1 isoforms of AChE could be gotten by size exclusion chromatography method.
Zhao
Qin
Q
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Tang
Xi-Can
XC
D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D002850:Chromatography, Gel; D007527:Isoenzymes / Q000302:isolation & purification*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D000110:Acetylcholinesterase