title: Results of human papillomavirus DNA testing with the hybrid capture 2 assay are reproducible.
abstract: Reproducibility of the Hybrid Capture 2 Test (HC 2) for human papillomavirus (HPV) DNA detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. Using a cutoff point of 1.0 pg of HPV DNA/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). Using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive and between low positive and high positive, respectively, the kappa was 0.68 and the linear-weighted kappa was 0.76. The results of this study indicate that HC 2 testing is reproducible even among cytologically normal women with low test values.
authors.affiliation: Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, USA. pc95p@nih.gov
authors.forename: Philip E
authors.initials: PE
authors.lastname: Castle
authors.forename: Attila T
authors.initials: AT
authors.lastname: Lorincz
authors.forename: Iwona
authors.initials: I
authors.lastname: Mielzynska-Lohnas
authors.forename: David R
authors.initials: DR
authors.lastname: Scott
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Glass
authors.forename: Mark E
authors.initials: ME
authors.lastname: Sherman
authors.forename: John E
authors.initials: JE
authors.lastname: Schussler
authors.forename: Mark
authors.initials: M
authors.lastname: Schiffman
mesh_terms: D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D015203:Reproducibility of Results; D019562:Viral Load
chemical_list: D004279:DNA, Viral