Safety of dietary supplements: chronotropic and inotropic effects on isolated rat atria.
We investigated the effects of dietary supplements on atria isolated from male Wistar rats. The examined supplements, which are increasingly used in Japan, those were Ginkgo biloba extract (GBE), catechins, isoflavones, sodium iron chlorophyllin and sodium copper chlorophyllin. GBE at 100-1000 microg/ml significantly increased the beat rate and the contractile force. Catechins at 1-100 microg/ml significantly potentiated the contractile force but did not effect the beat rates. However, isoflavones, sodium iron and sodium copper chlorophyllins did not change the contractile force or the beat rates. To identify the active ingredient of GBE, ginkgolide B, quercetin and amentoflavone on the atria were tested. Ginkgolide B weakened the contractile force. Quercetin potentiated the contractile force at only 30 microg/ml. Amentoflavone significantly increased the beat rate. From these findings, amentoflavone and quercetin were considered to be the principal ingredients of GBE producing the positive chronotropic and inotropic actions, respectively. In the case of catechins, (-)-epigallocatechin gallate (EGCg), one of the principal ingredients, produced inotropic actions. These findings suggest that there are some dietary supplements which affect cardiac function, such GBE and catechins.
Kubota
Yoko
Y
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Umegaki
Keizo
K
Tanaka
Naoko
N
Mizuno
Hideya
H
Nakamura
Kazuki
K
Kunitomo
Masaru
M
Shinozuka
Kazumasa
K
D000818:Animals; D002392:Catechin / Q000009:adverse effects; D019587:Dietary Supplements / Q000009:adverse effects*; D020441:Ginkgo biloba; D006339:Heart Rate / Q000187:drug effects*; D066298:In Vitro Techniques; D007529:Isoflavones / Q000009:adverse effects; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D007529:Isoflavones; D002392:Catechin