[Studies on changes in levels of hormone and expression of ob gene during induction of obesity in rats].
OBJECTIVE
To study the rules of changes in ob mRNA and its relation to hormone level in vivo in rats during their dynamic process to obesity induced by high-caloric fodder.


METHODS
Level of ob mRNA in adipose tissues of rats was determined with dot-hybridization techniques, and levels of serum insulin and growth hormone were measured with radioimmunoassay.


RESULTS
Level of ob mRNA in the fatty tissues and serum insulin level increased, but serum level of growth hormone decreased, with the increase in body weight of rats.


CONCLUSIONS
Increase in expression of ob mRNA could be the result, but not the cause, of obesity. Changes in the levels of ob mRNA, serum insulin and growth hormone indicated that they could affect each other and could be regulated by common metabolic signals.
Zhang
H
H
Institute for Environmental Health Inspection and Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021.
Sun
C
C
Li
C
C
Wang
S
S
Chen
B
B
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002149:Energy Intake; D005260:Female; D013006:Growth Hormone / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D020738:Leptin; D012333:RNA, Messenger; D013006:Growth Hormone