title: Unloaded shortening velocity and myosin heavy chain variations in human laryngeal muscle fibers.
abstract: Myosin description in human laryngeal muscles is incomplete, but evidence suggests the presence of type I, IIA, IIX, and tonic myosin heavy chain (MHC) fibers. This study describes the unloaded shortening velocity (V0) of chemically skinned laryngeal muscle fibers measured by the slack test method in relation to MHC content. Skeletal fibers from human laryngeal and limb muscle biopsy specimens were obtained for determination of V0, and subsequently, glycerol-sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to determine the MHC isoform content. The fibers from human limb muscle had shortening speeds similar to those in previous reports on human skeletal fibers. Type I, IIA, and IIX fibers of laryngeal muscle had shortening speeds similar to those of fibers from limb muscle, but laryngeal fibers with heterogeneous MHC expression had a wide range of shortening speeds, some being nearly twice as fast as limb fibers. In addition, MHC isoform bands from human extraocular muscle comigrated with some bands from laryngeal muscle--a finding suggesting that extraocular myosin may also be expressed.
authors.affiliation: Department of Orthodontics, University of Pittsburgh School of Dental Medicine, Pennsylvania 15261-1032, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Sciote
authors.forename: Terence J
authors.initials: TJ
authors.lastname: Morris
authors.forename: Carla A
authors.initials: CA
authors.lastname: Brandon
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Horton
authors.forename: Clark
authors.initials: C
authors.lastname: Rosen
mesh_terms: D000328:Adult; D000368:Aged; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D007821:Laryngeal Muscles / Q000737:chemistry / Q000502:physiology*; D007866:Leg; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology; D018995:Myosin Heavy Chains / Q000032:analysis*; D020033:Protein Isoforms
chemical_list: D020033:Protein Isoforms; D018995:Myosin Heavy Chains