[Apoptosis of HL60 cells induced by inhibition of bcl-2 with antisense phosphorothioate oligodeoxynucleotides].
OBJECTIVE
To observe the effects of different bcl-2 antisense phosphorothioate oligodeoxynucleotide (ASPO) concentrations in inducing apoptosis of HL60 cells.


METHODS
Signs of apoptotic cells were detected by acridine orange staining, transmission electron microscope, flow cytometry and DNA fragment electrophoresis.


RESULTS
In presence of ASPO, the apoptosis of HL60 cells could be induced after 24 hours of incubation. The apoptosis rate at ASPO 5, 10 and 20 micromol/L were (9.67 +/- 2.16)%, (18.25 +/- 3.01)% and (23.42 +/- 2.75)% respectively. There was a statistically significant increase in apoptosis as the dose of ASPO increased. After incubation for 72 hours, the apoptotic rates of the 3 different ASPO concentration groups were (9.58 +/- 2.96)%, (29.58 +/- 4.04)% and (36.33 +/- 3.66)% respectively, of which the apoptic rate of HL60 cells incubated with ASPO 5 micromol/L was highest at 48 hours.


CONCLUSIONS
Bcl-2 ASPO can specifically induce apoptosis of HL60 cells. Its effect depends upon the concentration of ASPO.
Lin
Y
Y
Affiliated Union Hospital, Fujian Medical University/Fujian Institute of Hematology, Fuzhou 350001.
Lu
L
L
Chen
Y
Y
D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D013873:Thionucleotides / Q000737:chemistry; D013997:Time Factors
D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D013873:Thionucleotides