Establishment of a convenient system for the long-term culture and study of non-neoplastic human salivary gland epithelial cells.
Epithelial cells appear to play an important role in the initiation and maintenance of autoimmune lesions in the salivary glands of patients with Sjogren's syndrome. Therefore, the detailed study of immunological function of salivary gland epithelial cells (SGEC) may provide useful information for the understanding of Sjögren's syndrome pathogenesis. In this report we aimed to formulate a protocol for the establishment of human non-neoplastic SGEC lines as a tool for the study of the physiology and pathophysiology of these cells. Pointing towards a practical approach, we sought to establish SGEC lines from quite a limited amount of biopsy tissue obtained during the diagnostic evaluation of patients. Herein, the favorable conditions for the long-term maintenance of human non-neoplastic SGEC lines are presented and involve the successive application of a serum-containing and a serum-free culture medium, supplemented with essential epithelial growth factors. This protocol has been found reliable and convenient, as attested by the reproducible establishment of non-neoplastic SGEC lines. The analysis of SGEC phenotypic features, as well as a coculture system for the study of interactions between epithelial cells and lymphocytes, are also described. Such techniques may provide valuable means for the functional and molecular investigation of human SGEC and particularly for the study of Sjögren's syndrome and other disorders of glandular epithelia.
Dimitriou
Ioannis D
ID
Department of Pathophysiology, School of Medicine, National University of Athens, Greece.
Kapsogeorgou
Efstathia K
EK
Abu-Helu
Rasmi F
RF
Moutsopoulos
Haralampos M
HM
Manoussakis
Menelaos N
MN
D017209:Apoptosis; D001327:Autoimmune Diseases / Q000473:pathology; D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002450:Cell Communication; D018929:Cell Culture Techniques; D002455:Cell Division; D018920:Coculture Techniques; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology; D006801:Humans; D015458:Leukemia, T-Cell / Q000473:pathology; D010641:Phenotype; D015203:Reproducibility of Results; D012469:Salivary Glands / Q000166:cytology* / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology; D013997:Time Factors
D003470:Culture Media; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor