Derivatization for liquid chromatography/electrospray mass spectrometry: synthesis of tris(trimethoxyphenyl)phosphonium compounds and their derivatives of amine and carboxylic acids.
A simple method for the derivatization of primary amines and carboxylic acids with tris(trimethoxyphenyl)phosphonium (TMPP) reagents to enhance their detection by electrospray mass spectrometry (ESI-MS) has been developed. The synthesis of novel TMPP reagents and their stable isotopically labelled analogues is described. Through the use of stable isotopically labelled TMPP "tags", incorporation of a doublet (1:1, (1)H/(2)H or (12)C/(13)C) into the target molecule can be achieved, enabling the use of isotopic target analysis to detect compounds of unknown molecular weight but with a characteristic isotope pattern and accurate mass difference.
Leavens
William J
WJ
Department of Metabolism and Pharmacokinetics, Glaxo Research and Development Ltd., Park Road, Ware, Hertfordshire SG12 0DP, UK. wjl34493@gsk.com
Lane
Stephen J
SJ
Carr
Richard M
RM
Lockie
Andrea M
AM
Waterhouse
Ian
I