title: Induction of dnaK through its native heat shock promoter is necessary for intramacrophagic replication of Brucella suis.
abstract: The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37 degrees C, with a thermal cutoff at 39 degrees C. However, the constitutive dnaK mutant, which showed high sensitivity to H(2)O(2)-mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France. kohler@crit.univ-montp2.fr
authors.forename: Stephan
authors.initials: S
authors.lastname: Köhler
authors.forename: Euloge
authors.initials: E
authors.lastname: Ekaza
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Paquet
authors.forename: Karl
authors.initials: K
authors.lastname: Walravens
authors.forename: Jacques
authors.initials: J
authors.lastname: Teyssier
authors.forename: Jacques
authors.initials: J
authors.lastname: Godfroid
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Liautard
mesh_terms: D000818:Animals; D002002:Brucella / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D002006:Brucellosis; D004338:Drug Combinations; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009821:Oils; D018384:Oxidative Stress; D010636:Phenols; D011401:Promoter Regions, Genetic; D013154:Spleen / Q000382:microbiology
chemical_list: D004338:Drug Combinations; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D009821:Oils; C027222:P & S Liquid; D010636:Phenols; C063842:dnaK protein, E coli