title: Cellular fatty acid composition from Sarcobium lyticum (Legionella lytica comb. nov.)--an intracellular bacterial pathogen of amoebae.
abstract: Legionella lytica comb. nov. an intracellular bacterial pathogen of small free-living amoebae was subjected to cellular fatty acid (FA) analysis employing base and acid catalyzed cleavage, gas-liquid chromatography and mass spectrometry. Both unbranched and branched (iso and anteiso) FA of chains ranging from 14 to 30 carbon atoms occurred. The presence of two long-chain FA: 27-oxo-octacosanoic acid and heptacosane-1,27-dioic acid, characteristic for legionellae, was found. Nine amide-linked 3-hydroxy-FA were revealed. The main 3-hydroxy-fatty acids comprise: 3-OH-14:0, 3-OH-16:0, 3-OH-18:0, 3-OH-i18:0, 3-OH-15:OH, 3-OH-i16:0 amd 3-OH-i17:0. The profile of hydroxy FAs permits allocation of L. lytica to group 3 of legionellae which comprise blue-white fluorescent species.
authors.affiliation: Department of General Microbiology, Maria Curie-Skłododska University, Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Palusińska-Szysz
authors.forename: A
authors.initials: A
authors.lastname: Choma
authors.forename: R
authors.initials: R
authors.lastname: Russa
authors.forename: W J
authors.initials: WJ
authors.lastname: Drozański
mesh_terms: D000656:Amoeba / Q000382:microbiology*; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007875:Legionella / Q000378:metabolism*
chemical_list: D005227:Fatty Acids