Sequence analysis of the complete genome of an iridovirus isolated from the tiger frog.
We have isolated a tiger frog virus (TFV) from diseased tiger frogs, Rana tigrina rugulosa. The genome was a linear double-stranded DNA of 105,057 basepairs in length with a base composition of 55.01% G+C. About 105 open reading frames were identified with coding capacities for polypeptides ranging from 40 to 1294 amino acids. Computer-assisted analyses of the deduced amino acid sequences revealed that 39 of 105 putative gene products showed significant homology to functionally characterized proteins of other species in the GenBank/EMBL/DDBJ databases. These proteins included enzymes and structural proteins involved in virus replication, transcription, modification, and virus--host interaction. The deduced amino acid sequences of TFV gene products showed more than 90% identity to FV3, but a low degree of similarity among TFV, ISKNV, and LCDV-1. The results from this study indicated that TFV may belong to the genus Ranavirus of the family Iridoviridae.
He
Jian G
JG
State Key Laboratory for Biocontrol, Zhongshan University, Guangzhou 510275, People's Republic of China. lsbrc05@zsu.edu.cn
Lü
Ling
L
Deng
Min
M
He
Hua H
HH
Weng
Shao P
SP
Wang
Xiao H
XH
Zhou
Song Y
SY
Long
Qin X
QX
Wang
Xun Z
XZ
Chan
Siu M
SM
D000595:Amino Acid Sequence; D000818:Animals; D016679:Genome, Viral; D006801:Humans; D007497:Iridoviridae / Q000235:genetics; D017994:Iridovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011898:Ranidae / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D014764:Viral Proteins