Heparan sulfate-mediated binding of infectious dengue virus type 2 and yellow fever virus.
Dengue virus type 2 and Yellow fever virus are arthropod-borne flaviviruses causing hemorrhagic fever in humans. Identification of virus receptors is important in understanding flavivirus pathogenesis. The aim of this work was to study the role of cellular heparan sulfate in the adsorption of infectious Yellow fever and Dengue type 2 viruses. Virus attachment was assessed by adsorbing virus to cells, washing unbound virus away, releasing cell-bound virus by freezing/thawing, and then titrating the released infectious virus. Treatment of cells by heparin-lyase, desulfation of cellular heparan sulfate, or treatment of the virus with heparin inhibited cell binding of both viruses. Heparin also inhibited Yellow fever virus infection by 97%. Using infectious virus, the present work shows the importance of heparan sulfate in binding and infection of these two flaviviruses.
Germi
Raphaële
R
Laboratoire de Virologie Moléculaire et Structurale EA 2939, Université Joseph Fourier, Grenoble, La Tronche, 38700, France.
Crance
Jean-Marc
JM
Garin
Daniel
D
Guimet
Josette
J
Lortat-Jacob
Hugues
H
Ruigrok
Rob W H
RW
Zarski
Jean-Pierre
JP
Drouet
Emmanuel
E
D000327:Adsorption; D000818:Animals; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003715:Dengue / Q000821:virology; D003716:Dengue Virus / Q000378:metabolism* / Q000472:pathogenicity*; D006497:Heparan Sulfate / Q000378:metabolism*; D014709:Vero Cells; D015004:Yellow Fever / Q000821:virology; D015005:Yellow fever virus / Q000378:metabolism* / Q000472:pathogenicity*
D006497:Heparan Sulfate