Metabolism of D-[1-(13)C]fructose, D-[2-(13)C]fructose, and D-[6-(13)C]fructose in rat hepatocytes incubated in the presence of H(2)O or D(2)O.
Isolated hepatocytes from fed rats were exposed for 120 min to D-[1-(13)C]fructose, D-[2-(13)C]fructose, or D-[6-(13)C]fructose in the presence of H(2)O or D(2)O. The identification and quantification of (13)C-enriched metabolites (D-glucose, L-lactate) in the incubation medium and the measurement of their deuterated isotopomers indicated that the ketohexose was phosphorylated predominantly at the intervention of fructokinase and that the majority of the D-glyceraldehyde molecules generated from d-fructose 1-phosphate were further metabolized, e.g., after phosphorylation to D-glyceraldehyde 3-phosphate. It is proposed that the present procedure may help to further characterize the regulation of D-fructose metabolism in both hepatocytes and other cell types.
Malaisse
Willy J
WJ
Laboratory of Experimental Medicine, Brussels Free University, Belgium. malaisse@med.ulb.ac.be
Ladrière
Laurence
L
Verbruggen
Ingrid
I
Willem
Rudolph
R
D000818:Animals; D002247:Carbon Isotopes; D002478:Cells, Cultured; D005632:Fructose / Q000378:metabolism* / Q000493:pharmacokinetics; D022781:Hepatocytes / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D014867:Water
D002247:Carbon Isotopes; D014867:Water; D005632:Fructose; D019344:Lactic Acid