[A study on the loss of heterozygosity of multiple tumor suppressor genes in gastrointestinal metaplasia].
OBJECTIVE
To investigate the role of multiple tumor suppressor gene abnormality in gastrointestinal metaplasia (IM).


METHODS
The loss of heterozygosity (LOH) of YNZ22, APC, MCC and DCC genes was examined by PCR-RFLP technique in 60 IM cases.


RESULTS
LOH of YNZ22, APC, MCC and DCC genes were found in 19.4% (6/31), 12.0% (3/25), 13.0% (3/23) and 4.3% (2/47) respectively. IM was classified into types I, II and III. It was found that the LOH rates of each tumor suppressor gene of type III IM were higher than those of types I & II IM, but the difference was not significant (P > 0.05). Two cases of type III IM were found to also have 2 gene alterations (APC, MCC or YNZ22, MCC) respectively. All LOH were found in IM of adjacent carcinoma. None were found in IM of cancer-free patients.


CONCLUSIONS
The alteration of tumor suppressor gene may play a role in the development and carcinogenesis of IM.
Wang
D
D
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038.
Fang
D
D
Liu
W
W
Luo
Y
Y
Lu
R
R
D004247:DNA / Q000235:genetics / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005767:Gastrointestinal Diseases / Q000235:genetics / Q000473:pathology*; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008679:Metaplasia; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA; D015247:Deoxyribonuclease HindIII; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases