[The role of Mac-1 and ICAM-1 molecules in adhesion of cells on fibronogen and its degradation products].
Monocytic cell adhesion to immobilized fibrinogen and fibrinogen degradation products, and involvement of integrins Mac-1 and immunoglobulin-like ICAM-1 adhesion molecules in these processes were investigated. Fibrinogen cleavage with plasmin down-regulated adhesion of cells with predominant Mac-1 expression; in contrast, the attachment of ICAM-1-expressing was up-regulated. By means of function-blocking anti-Mac-1 and anti-ICAM-1 antibodies, and immobilization of known fibrinogen degradation products, it was shown that Mac-1 molecules mediated cell adhesion predominantly to fibrinogen, and its early degradation products, fragments X and Y, while ICAM-1 participated in cell attachment to X- and Y-fragments, rather than to intact fibrinogen or late degradation products, fragments D and E.
Aref'eva
T I
TI
Institute of Experimental Cardiology, Russian Cardiology Research Complex, Moscow.
Baĭdun
L V
LV
Aref'eva
A M
AM
Krasnikova
T L
TL
D002448:Cell Adhesion; D002460:Cell Line; D005338:Fibrin Fibrinogen Degradation Products; D005340:Fibrinogen; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D015854:Up-Regulation
D005338:Fibrin Fibrinogen Degradation Products; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1; D005340:Fibrinogen