title: [The change in resistance of Pseudomonas aeruginosa to gentamicin during in vitro subculturing].
abstract: OBJECTIVE
  To observe the change in resistance of Pseudomonas aeruginosa (PA) to gentamicin (GM) during in vitro subculturing.
  
  
  METHODS
  Twenty -- six strains of PA with resistance to GM isolated from burn patients were subcultured in vitro for 30 generations. The strains were also divided into high level (20 strains) and medium level (6 strains) of resistant groups according to their MIC (minimum inhibition concentration) values. The MIC and MBC (minimal bactericidal concentration) levels were examined in every strains of the 1st, 10th, 20th, and 30th filial generations of PA strain.
  
  
  RESULTS
  There was no significant general difference of MIC and MBC values among the filial generations in each group (P < 0.05) when multiple comparisons of MIC and MBC mean values were made among every filial generations in high and medium levels of GM resistant groups.
  
  
  CONCLUSIONS
  There was no evident difference in GM resistance among all the filial generations of PA. The result indicated that, the PA with different levels of GM resistance clinically found might come from different PA strains.
authors.affiliation: Department of Burns and Plastic Surgery, Guangzhou Red Cross Hospital (The Fourth Affiliated Hospital, Medical College of Jinan University), Guangzhou Research Institute of Traumatic Surgery, Guangzhou, 510220 Guang Dong Province, P.R. China.
authors.forename: L
authors.initials: L
authors.lastname: Dai
authors.forename: F
authors.initials: F
authors.lastname: Ma
authors.forename: Y
authors.initials: Y
authors.lastname: Peng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002056:Burns / Q000382:microbiology; D002455:Cell Division / Q000187:drug effects; D024881:Drug Resistance, Bacterial; D005839:Gentamicins / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins