[Myocardial apoptosis induced by delayed fluid resuscitation in a burned rat model].
OBJECTIVE
To explore the possibility and the mechanism of myocardial apoptosis induced by delayed fluid resuscitation in a burned rat model and its relationship with nitric oxide (NO) and oxygen-derived free radicals.


METHODS
In a rat model with 30% III degree burn, the genomic DNA of the myocardial tissue was detected with ApoAlert(TMLM)-PCR Ladder Assay Kit and visualized with agarose gel electrophoresis. Meanwhile, the NO and the content of unsaturated fatty acids were measured.


RESULTS
In rats receiving delayed fluid resuscitation following burn, the myocardial genomic DNA exhibited DNA ladder-index of apoptosis, and the contents of myocardial NO and unsaturated fatty acid were much lower than those in rats receiving immediate resuscitation (P < 0.05).


CONCLUSIONS
The myocardial tissue undergoes apoptosis in burned rats receiving delayed fluid resuscitation, and the decreased NO and the production of oxygen-derived free radicals are also observed in this process.
Wang
G
G
Burns Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Yu
B
B
Xia
Z
Z
D000818:Animals; D017209:Apoptosis; D002056:Burns / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005440:Fluid Therapy; D008297:Male; D009206:Myocardium / Q000473:pathology*; D009569:Nitric Oxide / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D005231:Fatty Acids, Unsaturated; D009569:Nitric Oxide