title: Electrical contacting of glucose oxidase by surface-reconstitution of the apo-protein on a relay-boronic acid-FAD cofactor monolayer.
abstract: A new methodology for the surface-reconstitution of apo-flavoenzymes on a relay unit (pyrroloquinoline quinone, PQQ) functionalized with a boronic acid ligand as a linker to native FAD was developed. The reconstitution of apo-glucose oxidase (apo-GOx) on the PQQ-FAD monolayer yields an electrically contacted enzyme-electrode.
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Maya
authors.initials: M
authors.lastname: Zayats
authors.forename: Eugenii
authors.initials: E
authors.lastname: Katz
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D001897:Boronic Acids; D005182:Flavin-Adenine Dinucleotide; D005949:Glucose Oxidase