title: Immuno-capture PCR for detection of Aeromonas hydrophila.
abstract: In this report, we describe the use of universal primer PCR (UPPCR) for the detection of 16S ribosomal RNA (rRNA) genes from Aeromonas hydrophila captured by anti-A. hydrophila antibody coupled to a microplate. The approach combining immuno-capture with UPPCR provides a quick, sensitive, and reproducible way for the detection of bacterial cells.
authors.affiliation: Department of Biology and the Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, PR China. wangpeng@xmu.edu.cn
authors.forename: Xuanxian
authors.initials: X
authors.lastname: Peng
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhang
authors.forename: Sanying
authors.initials: S
authors.lastname: Wang
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Lin
authors.forename: Weiyi
authors.initials: W
authors.lastname: Zhang
mesh_terms: D016980:Aeromonas hydrophila / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000907:Antibodies, Bacterial; D017931:DNA Primers; D007118:Immunoassay; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000907:Antibodies, Bacterial; D017931:DNA Primers