[A study on the mechanism of the biological roles of danshensu on fibroblast].
OBJECTIVE
To explore the effects of danshensu on fibroblast apoptosis and the expression of procollagen gene.


METHODS
Danshensu was added to in vitro culture of human cutaneous fibroblasts, and the nuclear proteins and total RNA were harvested from the cells. Electrophoretic mobility shift assay (EMSA) was adopted to determine the combining activity of nuclear transcription factors NF-kB and NF-1. Cell apoptosis was analyzed by DNA ladder fragmentation. The procollagen gene expression was analyzed by RT-PCR.


RESULTS
Eight hours after the action of danshensu on the culture fibroblasts, the NF-kB combining activity was almost inhibited completely and the NF-1 combining activity decreased by about 50%. In addition, ladder-like DNA fragments were revealed clearly by sepharose electrophoresis. The mRNA levels of type I procollagen alpha1 and alpha2 decreased by 56% and 59%, respectively.


CONCLUSIONS
Danshensu could inhibit the nuclear transcription factor NF-kB activity of fibroblast and induce the occurrence of its apoptosis. Furthermore, danshensu could also inhibit the nuclear transcription factor NF-1 activity of fibroblast and modulate the synthesis and secretion of collagen.
Jiang
H
H
Department of Burns and Plastic Surgery, Nanjing Army General Hospital, Nanjing, 210002 P. R. China.
Ha
T
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Wei
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D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D053938:DNA Fragmentation / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D024042:Collagen Type I; D004268:DNA-Binding Proteins; D007773:Lactates; D016328:NF-kappa B; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C080333:transcription factor nuclear factor 1; C035055:3,4-dihydroxyphenyllactic acid