Boron compounds against human leukemic cells.
Two new boron compoumds, dihydroxy(oxybiguanido) boron (iii) hydrochloride monohydrate (HB) and guanidine biboric acid adduct (GB) were used in this study to observe the antitumor effect. Leukemic blast cells isolated from chronic myeloid leukemia (CML) patients showed significant cell growth inhibition within twentyfour hours. IC50 of GB and HB was 2mg/ml. The metabolically active cells were found to be inhibited by drug treatment as assessed by MTT test. Inhibition of 3H Thymidine incorporation also supported the above result. In this study we investigated the molecular mechanisms by which HB and GB induce apoptosis in immature blast cells.
Murmu
N
N
Chittaranjan National Cancer Institute, Calcutta, India.
Mitra
S
S
Das
M
M
Gomes
A
A
Vedasiromoni
J R
JR
Ghosh
M
M
Bhattacharya
M
M
Ghosh
P
P
Biswas
J
J
Bhattacharya
S
S
Sur
P
P
D017209:Apoptosis / Q000187:drug effects*; D001896:Boron Compounds / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects; D020298:U937 Cells / Q000187:drug effects
D001896:Boron Compounds; D004273:DNA, Neoplasm; D006146:Guanidines; D013778:Tetrazolium Salts; D013844:Thiazoles; C121738:dihydroxy(oxybiguanido)boron (III); C117044:guanidine biboric acid adduct; C022616:thiazolyl blue; D013936:Thymidine