Inhibition of translation and progesterone-induced maturation of Xenopus oocytes by expressing the amino-terminal portion of the eukaryotic translation initiation factor 4G.
The eukaryotic translation initiation factor 4G (eIF4G) plays a pivotal role in translation. EIF4G interacts with several other factors including eIF4E, which is a cap-binding protein, and the poly(A)-binding protein (PABP). In this work, we demonstrate that the expression of the amino-terminal one-third of eIF4G, which interacts with eIF4E and PABP, in Xenopus oocyte inhibits translation and progesterone-induced maturation.
Wakiyama
Motoaki
M
Institute for Biomolecular Science, Gakushuin University, Tokyo, Japan.
Miura
Kin-ichiro
K
D000818:Animals; D005057:Eukaryotic Cells; D039603:Eukaryotic Initiation Factor-4G; D015870:Gene Expression; D006801:Humans; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000235:genetics*; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger; D014981:Xenopus
D039603:Eukaryotic Initiation Factor-4G; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D011374:Progesterone