The role of donor CD4(+) T cells in the reconstitution of oral immunity by herpes simplex virus type 1 in severe combined immunodeficiency mice.
Severe combined immunodeficiency (SCID) mice with ill-developed Peyer's patches develop neither antibodies nor protection against lethal herpes simplex virus type 1 (HSV-1) infection by oral immunization. However, SCID mice carrying spleen cells from immunocompetent BALB/c mice had serum anti--HSV-1 antibody; anti--HSV-1 IgA antibody was detected in eye wash samples, and the mice were protected against lethal HSV-1 infection (88% survival rate). Western blotting showed that antibodies in SCID mice carrying spleen cells from BALB/c mice recognized 60-kDa HSV-1. The effector cells in transferred spleen cells were CD4(+), not CD8(+), T cells. Donor T cells were detected in the submucosal layer of the gut in SCID mice 1 day after transfer. Rapid movement of donor T cells to the gut may have a role in mucosal immunity to HSV-1. Thus, the normal environment for mucosal immunity develops in SCID mice without prior presence of CD4(+) T cells.
Irie
Hiroshi
H
Department of Pathology, Teikyo University School of Medicine, Tokyo, 173-8605, Japan. h-irie@rb3.so-net.ne.jp
Aita
Kiyoshi
K
Koyama
A Hajime
AH
Fukuda
Akio
A
Yoshida
Takeshi
T
Shiga
Junji
J
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018928:Immunity, Mucosal; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D010581:Peyer's Patches / Q000502:physiology*; D013154:Spleen / Q000473:pathology
D000914:Antibodies, Viral