[The mechanism of p16 gene inactivation in human bladder transitional cell carcinoma].
OBJECTIVE
To figure out the mechanism of p16 gene inactivation in human bladder transitional cell carcinoma (BTCC).


METHODS
p16 gene was analyzed for genetic alterations by Southern blot analysis, PCR-SSCP and DNA methylation analysis in 52 cases of transitional cell carcinoma of bladder.


RESULTS
p16 gene deletion was found in 11 of 52 (21.15%) BTCCs, only one tumor showed point mutation on p16 exon 2, nineteen cases of BTCCs (36.53%) showed p16 gene 5' CpG island methylation.


CONCLUSIONS
There was a statistically significant association between p16 gene deletion and early stage, well differentiated tumors. Additionally, a statistically significant correlation was also noticed between p16 gene 5' CpG island methylation and tumors in late stage with poor cell differentiation.
Wu
Y
Y
Tianjin Institute of Urological Surgery, Tianjin 300211.
Wu
H
H
Ma
T
T
Wu
C
C
D015139:Blotting, Southern; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020868:Gene Silencing; D006801:Humans; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm