Opsonophagocytosis of fluorescent polystyrene beads coupled to Neisseria meningitidis serogroup A, C, Y, or W135 polysaccharide correlates with serum bactericidal activity.
We developed a polysaccharide-specific flow cytometric opsonophagocytic assay (OPA) for the simultaneous measurement of functional antibody to Neisseria meningitidis serogroups A, C, Y, and W135. OPA titers significantly correlated with serum bactericidal assay titers for all serogroups tested (mean r = 0.96; P < 0.001). OPA could be used in meningococcal vaccine evaluation.
Martinez
Joseph
J
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jmartinez@cdc.gov
Pilishvili
Tamara
T
Barnard
Suzanne
S
Caba
Joseph
J
Spear
Willie
W
Romero-Steiner
Sandra
S
Carlone
George M
GM
D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis*; D001770:Blood Bactericidal Activity / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D008589:Meningococcal Infections / Q000276:immunology* / Q000517:prevention & control; D022401:Meningococcal Vaccines; D008863:Microspheres; D009345:Neisseria meningitidis / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D011137:Polystyrenes; D012703:Serotyping
D000907:Antibodies, Bacterial; D005456:Fluorescent Dyes; D022401:Meningococcal Vaccines; D011137:Polystyrenes