Determination of phenolic xenoestrogens in water by liquid chromatography with coulometric-array detection.
A sensitive method for the simultaneous determination of phenolic xenoestrogens such as bisphenol A, 2,4-dichlorophenol, 4-tert,-butylphenol, 4-n2-pentylphenol, 4-n-hexylphenol, 4-n-heptylphenol, 4-octylphenol, 4-nonylphenol was developed using reversed-phase LC and coulometric-array detection. Stepwise gradient elution with phosphoric acid in water-acetonitrile was used. The calibration curves were linear in the range of 5.0 (or 10.0)-1000 ng ml(-1) with correlation coefficients of 0.9978-0.9999, the limits of detection were 0.01-0.02 ng ml(-1). Sample clean-up was performed by solid-phase extraction (SPE) using 3M Empore extraction disks. Three commercial sorbents, C18, SDB-XD (styrene-divinylbenzene polymer) and SDB-RPS (sulfonated styrene-divinylbenzene polymer) were compared. The highest recoveries were obtained with SDB-RPS. They were above 70% with a relative standard deviation of less than 15%. The proposed method was applied to the determination of phenolic xenoestrogens in various water samples.
Inoue
Koichi
K
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
Yoshie
Yuriko
Y
Kondo
Sachiko
S
Yoshimura
Yoshihiro
Y
Nakazawa
Hiroyuki
H
D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004968:Estrogens, Non-Steroidal; D010636:Phenols; D014874:Water Pollutants, Chemical