title: LC determination of praziquantel in human plasma.
abstract: A simple high-performance liquid chromatographic (HPLC) method for the determination of praziquantel in human plasma was developed and validated. The present method was described by adding drop-wise 0.2 M Zinc sulfate and acetonitrile to plasma sample for deproteinization. This method used a reversed-phase Spherisorb ODS 2 column (5 microm), 250 x 4.6 mm i.d. as a stationary phase with a mobile phase consisting of acetonitrile- methanol-water (36:10:54, v/v/v), a flow rate of 1.5 ml/min and UV detection wavelength of 217 nm. Diazepam was used as internal standard. The standard calibration curve was linear over the concentration range of 100-2000 ng/ml (r=0.999). The equation of a linear regression line was y=8.05E-04+7.25E-04x with slope and intercept values of 0.0007 and 0.0008, respectively. The limit of detection was 12.25 ng/ml and the limit of quantification was set at 100 ng/ml. The intra- and inter-day assay coefficients of variation (CV) were 3.0+/-1.7 and 6.3+/-1.9%, respectively. The percentage of recovery was 102.1+/-5.6. Therefore, the HPLC method described here was simple, rapid and reproducible since it did not require extraction and evaporation processes in sample preparation, which will reduce time-consuming or expensive sample preparation.
authors.affiliation: Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla, Thailand. rwibool@ratree.psu.ac.th
authors.forename: Wibool
authors.initials: W
authors.lastname: Ridtitid
authors.forename: Malinee
authors.initials: M
authors.lastname: Wongnawa
authors.forename: Werawath
authors.initials: W
authors.lastname: Mahatthanatrakul
authors.forename: Jarurat
authors.initials: J
authors.lastname: Punyo
authors.forename: Methi
authors.initials: M
authors.lastname: Sunbhanich
mesh_terms: D000871:Anthelmintics / Q000097:blood; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D004355:Drug Stability; D006207:Half-Life; D006801:Humans; D011223:Praziquantel / Q000097:blood*
chemical_list: D000871:Anthelmintics; D011223:Praziquantel