title: Derivatization for liquid chromatography/electrospray mass spectrometry: synthesis of tris(trimethoxyphenyl)phosphonium compounds and their derivatives of amine and carboxylic acids.
abstract: A simple method for the derivatization of primary amines and carboxylic acids with tris(trimethoxyphenyl)phosphonium (TMPP) reagents to enhance their detection by electrospray mass spectrometry (ESI-MS) has been developed. The synthesis of novel TMPP reagents and their stable isotopically labelled analogues is described. Through the use of stable isotopically labelled TMPP "tags", incorporation of a doublet (1:1, (1)H/(2)H or (12)C/(13)C) into the target molecule can be achieved, enabling the use of isotopic target analysis to detect compounds of unknown molecular weight but with a characteristic isotope pattern and accurate mass difference.
authors.affiliation: Department of Metabolism and Pharmacokinetics, Glaxo Research and Development Ltd., Park Road, Ware, Hertfordshire SG12 0DP, UK. wjl34493@gsk.com
authors.forename: William J
authors.initials: WJ
authors.lastname: Leavens
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Lane
authors.forename: Richard M
authors.initials: RM
authors.lastname: Carr
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Lockie
authors.forename: Ian
authors.initials: I
authors.lastname: Waterhouse