[Effects of different catechins on cell cycle arrest and induction of apoptosis in LoVo cells].
OBJECTIVE
To investigate the effects of green tea (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin-3-gallate (ECG)and (-)-epicatechin (EC) on the cell cycle arrest and induction of apoptosis in human colon carcinoma LoVo cell line.


METHODS
The cytotoxicity, cell cycle arrest and apoptosis-associated alterations in morphology and biochemistry were examined using the tetrazolium salt reduction (MTT) assay, gel electrophoresis, transmission electron microscope and flow cytometry after treatment of LoVo cells with the four catechins.


RESULTS
Treatment of LoVo cells with EGCG and EGC resulted in the growth suppression and induction of apoptosis in a time- and concentration-dependent manner. ECG and EC, however, did not have the same effects. In addition, the four catechins had different effects on the cell cycle of LoVo cells. Treatment with EGCG, EGC and ECG caused LoVo cells arrest at G1 phase in the cell cycle progression, whereas EC resulted in an arrest at S phase.


CONCLUSIONS
The cytotoxicity of the four catechins to LoVo cells is significantly different. The difference is associated with their effects on cell cycle and induction of apoptosis in LoVo cells.
Tan
X
X
Institute for Hepatic Diseases, Beijing Army General Hospital, Beijing 100700, China.
Zhou
D
D
Zhang
Y
Y
Jiang
B
B
D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans
D002392:Catechin; C062669:epicatechin gallate; C045651:epigallocatechin gallate