Glutathione S-transferase-pi in malignant tissues and plasma of human colorectal and gastric cancers.
OBJECTIVE
In this study, 16 paired samples of colorectal and gastric cancers and adjacent non-neoplastic tissues were analysed for the determination of glutathione S-transferase (GST) activities and the expression of GST-pi.


METHODS
Western blotting procedure as well as plasma GST-pi levels were used.


RESULTS
GST activities were found to be increased in malignant tissues of patient compared with adjacent normal tissues. A significant correlation was detected between GST activity and GST-pi expression in malignant tissues of patients. Plasma GST-pi levels increased in patients compared to aged-matched control subjects. When the patients were grouped according to TNM stage, GST-pi expression in malignant tissues as well as plasma GST-pi levels were higher in patients with more advanced tumor stages.


CONCLUSIONS
Our results indicate that GST-pi expression in malignant tissues and plasma GST-pi levels in human colorectal and gastric cancers increased depending on the stages of tumor.
Doğru-Abbasoğlu
S
S
Department of Biochemistry, Istanbul Medical Faculty, University of Istanbul, Capa, 34390, Istanbul, Turkey. abbasoglusl@hotmail.com
Mutlu-Türkoğlu
U
U
Türkoğlu
S
S
Erbil
Y
Y
Barbaros
U
U
Uysal
M
M
Aykaç-Toker
G
G
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase