Quencher as leaving group: efficient detection of DNA-joining reactions.
We describe a new fluorescence reporting strategy in which dabsyl, a well-known quencher, activates a hydroxyl group in a probe to convert it to a leaving group. When a nucleophilic phosphorothioate probe binds adjacent to a dabsyl quenched probe, autoligation occurs, releasing the quencher, and lighting up the probes, This signal change can be used to detect single nucleotide differences in DNA without enzymes or reagents.
Sando
Shinsuke
S
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Kool
Eric T
ET
D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011905:Genes, ras; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
D015342:DNA Probes; D005456:Fluorescent Dyes; D004247:DNA