title: [Antigen loading on dendritic cells affects the cell function in stimulating T cells].
abstract: OBJECTIVE
  To study the effect of antigen loading on dendritic cells (DC).
  
  
  METHODS
  DCs collected from peripheral blood monocytes were loaded with a tumor antigen from XG-7 cell line. These DCs were then co-cultured with allogeneic T cells and were compared with those DCs without antigen exposure.
  
  
  RESULTS
  Although DCs showed no change in their phenotypes after cultured with the antigen, they secreted more IL-12, and became more powerful in allogenic mixed lymphocyte reaction (MLR). Antigen-loaded DC stimulated more CD(4)(+) cells to proliferate than nonantigen-loaded DCs did. These CD(4)(+) cells did not kill XG-7 cells, but promoted CD(8)(+) cells' ability to inhibit the XG-7 proliferation.
  
  
  CONCLUSIONS
  The proliferation of CD(4)(+) cells after cultured with DC may become an indicator for the function of antigen loaded DC and for the efficiency of DC immunotherapy.
authors.affiliation: Department of Immunology, Suzhou Medical University, Suzhou 215007, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Qiu
authors.forename: Z
authors.initials: Z
authors.lastname: Gu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D018664:Interleukin-12 / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D018664:Interleukin-12