[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice].
OBJECTIVE
To observe the influence of transfected soluble vascular endothelial growth factor (VEGF) receptor (sFlt-1) gene on K562 leukemia cell growth in vivo.


METHODS
(1) The binding region of VEGF receptor (Flt-1) ligand was combined with fragment of IgH stable region to construct Flt-Ig fusion gene and insert into pcDNA3 vector. (2) By using electroporation, the pcDNA3/Flt-Ig was transfected into K562 leukemia cells, and selected by G418. Flt-Ig mRNA expression was detected by RT-PCR. (3) The transfected pcDNA3/Flt-Ig and pcDNA3-Ig K562 cells were respectively transplanted into nude mice and the tumor volume was dynamically measured.


RESULTS
Five subclones of K562 cells with high expression of Flt-Ig gene have been established, one of them was transplanted into 6 nude mice. The tumor volume of experimental mice was obviously smaller than that of control mice, about one half of the control group (P < 0.05).


CONCLUSIONS
The growth of transfected pcDNA3/Flt-Ig K562 cells was significantly inhibited. It is possible that soluble Flt-Ig protein secreted from K562/Flt-Ig cells neutralized VEGF produced from tumor cells, therefore inhibited the tumor angiogenesis.
Zhao
J
J
Institute of Hematology, CAMS & PUMC, State key Lab. of Experimental Hematology, Tianjin 300020, China.
Chu
J
J
Wang
S
S
Xu
S
S
D000818:Animals; D049109:Cell Proliferation; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics* / Q000378:metabolism; D020014:K562 Cells; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D047368:Tumor Burden; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics* / Q000378:metabolism
D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1