title: Three classes of proteinase inhibitor gene have distinct but overlapping patterns of expression in Pisum sativum plants.
abstract: Genes representative of three gene classes encoding proteinase inhibitor proteins, with distinct spatial expression patterns, were isolated and characterized from Pisum. Under standard plant growth conditions, one class is expressed exclusively in seeds, whereas the other two make minor contributions to seed inhibitor proteins but are also expressed in other organs, predominantly in root endodermal and floral reproductive tissues. Two of the gene classes contain few genes and are genetically linked at the Tri locus, whereas the third class displays complex hybridization patterns to genomic DNA and maps to diverse genetic loci. Expression analysis of this last class suggests that only a small number of these genes are expressed. The quantitative effect of the Tri locus on root and floral inhibitor gene expression was examined in near-isogenic lines of pea. The proteins encoded by the three classes are all members of the same family (Bowman-Birk) of enzyme inhibitors but are distinct in terms of overall sequence, active site sequences and inhibitor function.
authors.affiliation: John Innes Centre, Norwich Research Park, UK. claire.domoney@bbsrc.ac.uk
authors.forename: Claire
authors.initials: C
authors.lastname: Domoney
authors.forename: Tracey
authors.initials: T
authors.lastname: Welham
authors.forename: Noel
authors.initials: N
authors.lastname: Ellis
authors.forename: Philippe
authors.initials: P
authors.lastname: Mozzanega
authors.forename: Lynda
authors.initials: L
authors.lastname: Turner
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics*; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D018514:Plant Structures / Q000201:enzymology / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000235:genetics*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D018749:RNA, Plant; D015842:Serine Proteinase Inhibitors