title: A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells.
abstract: Myosin-1c (also known as myosin-Ibeta) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N(6)-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.
authors.affiliation: Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Holt
authors.forename: Susan K H
authors.initials: SK
authors.lastname: Gillespie
authors.forename: D William
authors.initials: DW
authors.lastname: Provance
authors.forename: Kavita
authors.initials: K
authors.lastname: Shah
authors.forename: Kevan M
authors.initials: KM
authors.lastname: Shokat
authors.forename: David P
authors.initials: DP
authors.lastname: Corey
authors.forename: John A
authors.initials: JA
authors.lastname: Mercer
authors.forename: Peter G
authors.initials: PG
authors.lastname: Gillespie
mesh_terms: D000244:Adenosine Diphosphate; D000818:Animals; D006198:Hair Cells, Auditory / Q000502:physiology*; D006309:Hearing / Q000235:genetics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D018995:Myosin Heavy Chains / Q000737:chemistry* / Q000235:genetics / Q000502:physiology
chemical_list: D020409:Molecular Motor Proteins; C491827:Myo1a protein, mouse; D000244:Adenosine Diphosphate; D018995:Myosin Heavy Chains