title: Porcine hepatic phospholipid efflux during reperfusion after cold ischemia.
abstract: BACKGROUND
  Cold preservation produces hepatic injury that is difficult to assess during early reperfusion. The value of reperfusion plasma choline phospholipid in predicting subsequent organ function is documented in these studies.
  
  
  METHODS
  Livers of female Yorkshire pigs were prepared for transplantation. After 2 h of cold ischemia the reperfusion plasma was evaluated for choline phospholipid and cholesterol. These values were correlated with bile secretion, hepatic hemodynamics, oxygen uptake, and plasma sorbitol dehydrogenase levels.
  
  
  RESULTS
  The isolated porcine liver demonstrates a rapid efflux of choline phospholipids into plasma during early reperfusion after cold preservation. After this initial efflux no subsequent plasma increment occurred. These choline-phospholipid increments were isolated in plasma higher density (d > 1.063) lipoproteins and were not accompanied by equivalent increases in cholesterol. Neither biliary reflux nor lecithin cholesterol acyl transferase abnormalities contributed appreciably to the phospholipid increments in reperfusion plasma. Livers with the largest efflux of choline phospholipids had the most impaired circulatory and bile secretory function at 4 h of reperfusion.
  
  
  CONCLUSIONS
  The immediate increase of choline phospholipids, particularly lysophosphatidylcholine, in reperfusion plasma after cold ischemia provides an index of the injury occurring during this interval and correlates with early organ function.
authors.affiliation: Department of Surgery, UMass Memorial Medical Center, Worcester, Massachusetts, 01655, USA.
authors.forename: Rocco
authors.initials: R
authors.lastname: Ricciardi
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Veal
authors.forename: Saif
authors.initials: S
authors.lastname: Anwaruddin
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Wheeler
authors.forename: David P
authors.initials: DP
authors.lastname: Foley
authors.forename: Susan E
authors.initials: SE
authors.lastname: Donohue
authors.forename: Steven H
authors.initials: SH
authors.lastname: Quarfordt
authors.forename: William C
authors.initials: WC
authors.lastname: Meyers
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003080:Cold Temperature; D005260:Female; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008102:Liver Circulation; D016031:Liver Transplantation; D008244:Lysophosphatidylcholines / Q000097:blood; D010101:Oxygen Consumption; D010477:Perfusion; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D010713:Phosphatidylcholines / Q000097:blood*; D015427:Reperfusion Injury / Q000378:metabolism*; D013552:Swine
chemical_list: D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D002784:Cholesterol; D007064:L-Iditol 2-Dehydrogenase; D007862:Phosphatidylcholine-Sterol O-Acyltransferase