[Research on the oncogenesis of cervical epithelial cells co-induced by human papillomavirus and human cytomegalovirus].
OBJECTIVE
The carcinogenesis of the human cervical carcinoma is known closely associated with human papillomavirus (HPV). The purpose of this article is to identify whether another sexually transmitted factor, human cytomegalovirus (HCMV) plays as a co-factor role in the carcinogenesis.


METHODS
Human cervical epithelial cells immortalized by HPV16 were transfected with IE gene of HCMV. The condition and efficiency of IE gene were examined.


RESULTS
It was showed that the cervical epithelial cells could be completely transformed into malignant by HPV 16 and IE gene of HCMV and tumor was formed by injecting the transformed cells in nude mice.


CONCLUSIONS
The co-operation effect of HPV and HCMV auailed the oncogenesis of human cervical carcinoma and moreover, the cellular and molecular biological mechanism was discussed.
Liu
C
C
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Han
G
G
Li
K
K
Si
J
J
Liu
S
S
Song
G
G
D000818:Animals; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics*; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005260:Female; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015534:Trans-Activators; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D015534:Trans-Activators