Variability in fatty acid and triacylglycerol composition of the oil of coconut (Cocos nucifera L.) hybrids and their parentals.
The fatty acid profiles and triacylglycerol (TAG) compositions of oils from the solid endosperm of different Philippine coconut hybrids and their parentals were determined by using gas chromatography (GC) and high-performance liquid chromatography (HPLC). In general, varietal differences in fatty acid composition were observed. Lauric acid (C12) content was significantly higher in the hybrids PCA 15-8 (50.45%) and PCA 15-9 (50.26%) by about 3.16% points as compared to other hybrids, and higher in Tacunan Green Dwarf (50.50%) among the parentals. Among the fatty acids, lauric acid exhibited the least variation. In general, none of the hybrids had higher fatty acid content than their parentals. The HPLC chromatogram of triacylglycerols (TAG) showed 8 major peaks which differ in carbon number (CN) by two: identified as TAG CN 30, 32, 34, 36, 38, 40, 42, and 44. TAGs CN 30 (4.08%) and CN 34 (19.20%) were found to be significantly higher in PCA 15-9 than in the other hybrids. CN 36 was highest (21.94-23.66%) in all hybrids and parentals. The TAG CNs varied significantly among hybrids and parents, i.e., in CN 30, 32, and 34, which are high in medium chain triacylglycerols (MCTs), and in CN 30 (for parentals only), 40, 42, and 44 (the latter two for parentals only), and none in CN 36. MCTs calculated for two hybrids and their parents ranged from 13.81% to 20.55%.
Laureles
Lucita R
LR
Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Baños, College 4031, Laguna, Philippines.
Rodriguez
Felicito M
FM
Reaño
Consorcia E
CE
Santos
Gerardo A
GA
Laurena
Antonio C
AC
Mendoza
Evelyn Mae Tecson
EM
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D000074263:Coconut Oil; D003059:Cocos / Q000737:chemistry* / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D006824:Hybridization, Genetic; D010938:Plant Oils / Q000737:chemistry*; D013045:Species Specificity; D014280:Triglycerides / Q000032:analysis*
D005227:Fatty Acids; D010938:Plant Oils; D014280:Triglycerides; D000074263:Coconut Oil