title: [Quantitative determination of EAIgG by ABC-ELISA and its clinical significance].
abstract: OBJECTIVE
  To explore a method for diagnosis of autoimmune hemolytic anemia (AIHA) and evaluate its clinical significance.
  
  
  METHODS
  Erythrocyte associated IgG (EAIgG) was quantified in 60 healthy people, 44 patients with warm antibody AIHA (WAIHA), 108 non-immune anemia and 44 other immune diseases (total 256 cases) by using ABC-ELISA were estimated.
  
  
  RESULTS
  EAIgG in WAIHA patients were significantly higher than those in other groups (P < 0.01). In the 44 WAIHA patients, 38 were Coombs positive and 6 Coombs negative. EAIgG in Coombs negative was significantly high. The dynamic changes of EAIgG in 12 WAIHA patients were observed during treatment. When patient's condition got better, Hb and RBC increased and EAIgG decreased.
  
  
  CONCLUSIONS
  Quantitative detection of EAIgG is an index in the diagnosis of WAIHA, especially in AIHA with negative Coombs' test. It is also a useful factor for evaluating therapeutic effect and prognosis in WAIHA.
authors.affiliation: Jingzhou City Central Hospital, Jingzhou, Hubei 434100, China.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Deng
mesh_terms: D000293:Adolescent; D000328:Adult; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood* / Q000276:immunology; D003298:Coombs Test; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged
chemical_list: D007074:Immunoglobulin G