title: [Effects of methyl tertiary butyl ether on cell cycle and cell apoptosis].
abstract: OBJECTIVE
  To explore the effects of the new gasoline additive, methyl tertiary butyl ether (MTBE) on cell cycle and cell apoptosis.
  
  
  METHODS
  Flow cytometry was used to evaluate the effect of MTBE (1, 2, 4 microl/ml, 24 h) on NIH/3T3 cell cycles; and the effect of MTBE on Hela cell apoptosis was evaluated by detecting cell survival using crystal violet staining.
  
  
  RESULTS
  Flow cytometry showed that MTBE could change NIH/3T3 cell cycles, decrease the number of cells in S stage, and arrest cells at G(2) + M stage. The results suggested that MTBE could affect NIH/3T3 cell cycles and induce cell proliferation. This situation existed 48 hours after the treatment, and cell cycles came back normal 96 hours after the treatment. By detecting cell survival using crystal violet staining, we found that MTBE could inhibit the apoptosis of Hela cells which was induced by tumor necrosis factor (TNF)alpha and cycloheximide.
  
  
  CONCLUSIONS
  MTBE's carcinogenicity to animals may relate to induction of cell proliferation and inhibition of cell apoptosis.
authors.affiliation: Department of Environmental Health, Shanghai Medical University, Shanghai 200032, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: G
authors.initials: G
authors.lastname: Huang
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Ye
mesh_terms: D016475:3T3 Cells; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008738:Methyl Ethers / Q000633:toxicity*; D051379:Mice
chemical_list: D000393:Air Pollutants; D002273:Carcinogens; D008738:Methyl Ethers; C043243:methyl tert-butyl ether