[Influence of chemotherapy on hematopoietic microenvironment and effect of autologous bone marrow stromal cell infusion on the recovery of hematopoiesis after chemotherapy].
OBJECTIVE
To observe the influence of chemotherapy on hematopoietic progenitor cells (HPC) and hematopoietic microenvironment (HME). To observe whether intravenous infusion of autologous bone marrow stromal cells (ABMSC) expanded in vitro can improve the hematopoietic function.


METHODS
Cultures of CFU-GM, BFU-E, CFU-E and stromal progenitor cells (CFU-F) from normal control and chemotherapeutic patients were performed. The stromal function was analyzed by the assessment of the area of flask surface covered by stromal cells (ASSC) and the time when stromal cells reach confluence (TC). The recovery of hematopoietic function in short term chemotherapy group and long term chemotherapy, with or without ABMSC infusion (1.1 - 8.7) x 10(8) post chemotherapy groups was observed.


RESULTS
The yields of CFU-GM, BFU-E, CFU-E and CFU-F in long term chemotherapy group were significantly lower than that in normal group or in short term chemotherapy group. There was no significant difference among three groups in the ASSC and TC. In long term chemotherapy group, the yields of CFU-GM, CFU-E, BFU-E and CFU-F after chemotherapy with BMSC infusion were significantly higher than that without BMSC infusion. In long term chemotherapy group, the lowest value of white blood cell (WBC) and platelet after chemotherapy with BMSC infusion was significantly higher than that without BMSC infusion. The times for WBC and platelets recovered to normal were significantly shorter in BMSC group than in without BMSC. No adverse reaction was observed with ABMSC infusion.


CONCLUSIONS
Long term chemotherapy results in severe impairment in HPC and mesenchymal progenitor cell (MPC), but has no obvious influence on the in vitro BMSC confluent layer formation. Intravenous infusion of expanded ABMSC can accelerate the recovery of hematopoiesis after chemotherapy.
Liu
J
J
Department of Hematology, Air General Hospital, Beijing 100036, China.
Ji
S
S
Zhou
L
L
Chen
H
H
D000293:Adolescent; D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000627:therapeutic use*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D006413:Hematopoietic System / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*; D014182:Transplantation, Autologous; D055815:Young Adult