Interaction of PRMT1 with BTG/TOB proteins in cell signalling: molecular analysis and functional aspects.
BACKGROUND
Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N-methyltransferase of type I).


RESULTS
First, we have studied the interaction between PRMT1 and the proteins of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell-derived neurones.


CONCLUSIONS
Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation-mediated signalling pathway as well as in neuronal differentiation.
Berthet
Cyril
C
Unité INSERM U453, affiliée au CNRS, Centre Léon Bérard, Le Cheney, 28 rue Laënnec, 69373 Lyon Cedex 08, France.
Guéhenneux
Fabienne
F
Revol
Valérie
V
Samarut
Christiane
C
Lukaszewicz
Agnès
A
Dehay
Colette
C
Dumontet
Charles
C
Magaud
Jean-Pierre
JP
Rouault
Jean-Pierre
JP
D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D057846:Cell-Penetrating Peptides; D019175:DNA Methylation; D017874:Immediate-Early Proteins / Q000502:physiology*; D009363:Neoplasm Proteins / Q000502:physiology*; D009474:Neurons; D016716:PC12 Cells; D011484:Protein-Arginine N-Methyltransferases / Q000502:physiology*; D051381:Rats; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D013234:Stem Cells
C489553:Btg1 protein, rat; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D017874:Immediate-Early Proteins; D009363:Neoplasm Proteins; C414409:penetratin; D011484:Protein-Arginine N-Methyltransferases