Determination of the pesticide napropamide in soil, pepper, and tomato by micelle-stabilized room-temperature phosphorescence.
A selective and sensitive method for determining napropamide by room-temperature phosphorescence in SDS micelles is proposed and applied to the determination of this substance in a technical formulation and in spiked soil, pepper, and tomato samples. The use of phosphorescence enhancers such as sodium dodecyl sulfate (micellar agent), thallium (I) nitrate (external heavy atom), and sodium sulfite (deoxygenation agent) was studied and optimized to obtain maximum sensitivity. The determination was performed in 66 mM SDS, 30 mM thallium (I) nitrate, and 8 mM sodium sulfite. Taking into account both maximum phosphorescence intensity and the time required to reach that, a pH value of 7.2 was selected. After the samples were left standing at room temperature for 10 min, the phosphorescence was totally developed. The intensity was then measured at lambda(ex) = 282 nm and lambda(em) = 528 nm. The calibration graph was linear for 50-600 ng mL(-1) napropamide. The detection limit, according to the error propagation theory, was 16 ng mL(-1). The method has been demonstrated for the analysis of soils, peppers, and tomatoes, but, because of matrix interference, the method of standard additions was applied to determine napropamide in the vegetable samples. Recoveries from all these matrixes of added napropamide were near 100%.
Murillo Pulgarín
José A
JA
Department of Analytical Chemistry and Foods Technology, University of Castilla La Mancha, 13071 Ciudad Real, Spain. jamurill@qata-cr.uclm.es
García Bermejo
Luisa F
LF
D002138:Calibration; D006540:Herbicides / Q000032:analysis*; D008163:Luminescent Measurements; D018551:Solanum lycopersicum / Q000737:chemistry*; D008823:Micelles; D009281:Naphthalenes / Q000032:analysis*; D029222:Piper nigrum / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012987:Soil / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D006540:Herbicides; D008823:Micelles; D009281:Naphthalenes; D012987:Soil; C010909:devrinol