A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression.
Recent molecular insights have established the podocyte as a key component of the glomerular filtration barrier, and hence an important common pathway in proteinuric diseases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the "permissive" temperature (33 degrees C). After transfer to the "nonpermissive" temperature (37 degrees C), they entered growth arrest and expressed markers of differentiated in vivo podocytes, including the novel podocyte proteins, nephrin, podocin, CD2AP, and synaptopodin, and known molecules of the slit diaphragm ZO-1, alpha-, beta-, and gamma-catenin and P-cadherin. The differentiation was accompanied by a growth arrest and the upregulation of cyclin-dependent kinase inhibitors, p27 and p57, as well as cyclin D(1), whereas cyclin A was downregulated. These data are consistent with cell cycle protein expression during podocyte maturation in vivo. In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease.
Saleem
Moin A
MA
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
O'Hare
Michael J
MJ
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Reiser
Jochen
J
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Coward
Richard J
RJ
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Inward
Carol D
CD
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Farren
Timothy
T
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Xing
Chang Ying
CY
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Ni
Lan
L
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Mathieson
Peter W
PW
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
Mundel
Peter
P
*Children's Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; LICR/UCL Breast Cancer Laboratory, London, UK; Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.
D015415:Biomarkers; D015820:Cadherins / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002461:Cell Line, Transformed; D002675:Child, Preschool; D003584:Cytological Techniques; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000166:cytology* / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
D015415:Biomarkers; D015820:Cadherins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C407223:NPHS2 protein; D011506:Proteins; C113267:nephrin