title: Selective in vivo depletion of CD4(+) T lymphocytes with anti-CD4 monoclonal antibody during acute infection of calves with Anaplasma marginale.
abstract: To investigate the in vivo role of CD4(+) T lymphocytes during acute anaplasmosis, thymectomized calves were selectively depleted of CD4(+) T lymphocytes by treatment with anti-CD4 monoclonal antibody (MAb) and were then infected with the Florida strain of Anaplasma marginale in two sequential experiments (experiments 1 and 2). Treatment of thymectomized calves with a total of 5.0 mg of anti-CD4 MAb/kg of body weight during the 1st week followed by 0.3-mg/kg doses administered twice weekly for 7 weeks resulted in significant depletion of CD3(+) CD4(+) and CD4(+) CD45R(+) (naive) T lymphocytes from blood, spleen, and peripheral lymph nodes for the duration of the 8-week study, compared to the results for thymectomized control calves treated with a subclass-matched MAb. All calves became parasitemic and pyretic following experimental infection with A. marginale, and decreases in packed cell volume (PCV) coincided with peak parasitemia. No significant differences in PCV or parasitemia were observed between treatment groups. Thymectomized calves treated with anti-CD4 MAb were able to mount an anti-A. marginale antibody response, although in experiment 2, anti-CD4 MAb-treated calves had four- to sixfold lower immunoglobulin G1 (IgG1) and no detectable IgG2 anti-A. marginale major surface protein 2-specific antibody titers compared to thymectomized control calves treated with a subclass-matched MAb. At the level of CD4(+)-T-lymphocyte depletion achieved and experimental anaplasmosis induced, thymectomized anti-CD4 MAb-treated calves were able to control acute anaplasmosis. This was in contrast to the prediction that significant depletion of CD4(+) T lymphocytes would abrogate resistance to acute infection.
authors.affiliation: Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Pullman, Washington 99163, USA. reg@vetmed.wsu.edu
authors.forename: Reginald A
authors.initials: RA
authors.lastname: Valdez
authors.forename: Travis C
authors.initials: TC
authors.lastname: McGuire
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Brown
authors.forename: William C
authors.initials: WC
authors.lastname: Davis
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Jordan
authors.forename: Donald P
authors.initials: DP
authors.lastname: Knowles
mesh_terms: D000208:Acute Disease; D000712:Anaplasmosis / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004912:Erythrocytes / Q000382:microbiology; D006400:Hematocrit; D007074:Immunoglobulin G / Q000097:blood; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D013154:Spleen / Q000166:cytology; D013934:Thymectomy
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G