title: Bactericidal activity of electrolyzed acid water from solution containing sodium chloride at low concentration, in comparison with that at high concentration.
abstract: Electrolyzed strong acid water (ESW) containing free chlorine at various concentrations is becoming to be available in clinical settings as a disinfectant. ESW is prepared by electrolysis of a NaCl solution, and has a corrosive activity against medical instruments. Although lower concentrations of NaCl and free chlorine are desired to eliminate corrosion, the germicidal effect of ESW with low NaCl and free-chlorine concentrations (ESW-L) has not been fully clarified. In this study, we demonstrated that ESW-L possesses bactericidal activity against Mycobacteria and spores of Bacillus subtilis. The effect was slightly weaker than that of ESW containing higher NaCl and free-chlorine concentrations (ESW-H), but acceptable as a disinfectant. To clarify the mechanism of the bactericidal activity, we investigated ESW-L-treated Pseudomonas aeruginosa by transmission electron microscopy, a bacterial enzyme assay and restriction fragment length polymorphism pattern (RFLP) assay. Since the bacterium, whose growth was completely inhibited by ESW-L, revealed the inactivation of cytoplasmic enzyme, blebs and breaks in its outer membrane and remained complete RFLP of DNA, damage of the outer membrane and inactivation of cytoplasmic enzyme are the important determinants of the bactericidal activity.
authors.affiliation: Department of Microbiology, Osaka Medical College, Takatsuki, Osaka 569-8686, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Kiura
authors.forename: Kouichi
authors.initials: K
authors.lastname: Sano
authors.forename: Shinichi
authors.initials: S
authors.lastname: Morimatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakano
authors.forename: Chizuko
authors.initials: C
authors.lastname: Morita
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Maeda
authors.forename: Yoji
authors.initials: Y
authors.lastname: Katsuoka
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects* / Q000502:physiology; D002713:Chlorine / Q000032:analysis; D004202:Disinfectants / Q000494:pharmacology*; D004572:Electrolysis / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009161:Mycobacterium / Q000187:drug effects*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology*; D012996:Solutions / Q000737:chemistry; D013171:Spores, Bacterial / Q000187:drug effects
chemical_list: D004202:Disinfectants; D012996:Solutions; D012965:Sodium Chloride; D002713:Chlorine