[Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization].
OBJECTIVE
To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type.


METHODS
Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells.


RESULTS
Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases.


CONCLUSIONS
The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL.
Zhang
Y
Y
Department of Haematology, the Second Hospital of Kunming Medical College, Kunming 650101, China.
Ho
F C
FC
Shen
D
D
Xu
W
W
Chan
A C
AC
Srivastava
G
G
D003937:Diagnosis, Differential; D005753:Gastric Mucosa / Q000737:chemistry; D005756:Gastritis / Q000378:metabolism; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000032:analysis* / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000032:analysis* / Q000235:genetics; D017403:In Situ Hybridization; D018442:Lymphoma, B-Cell, Marginal Zone / Q000737:chemistry*; D010950:Plasma Cells / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D012333:RNA, Messenger