[The effects of rhGH on macrophages in scalded mice].
OBJECTIVE
To investigate the effects of rhGH and IGF-1 on the CD14 mRNA expression and cytokine secretion of peritoneal macrophages (Mphi) in scalded rats.


METHODS
Reverse-transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosordent assay (ELISA) were employed to observe the postburn changes of CD14 expression and cytokines secretion of the Mphi treated in vivo and in vitro by rhGH and IGF-1.


RESULTS
It was demonstrated that in vivo postburn application of rhGH and IGF-1 could enhance the transcription of CD14 mRNA and the secretion of cytokines from the peritoneal Mphi in mice. But it seemed that there was no additive effect between the two factors. Additionally, rhGH (at 40 ng/ml) and IGF-1 (at all tested concentrations) could activate the secretion of TNF and IL-6 from cultured mice Mphi in vitro.


CONCLUSIONS
Postburn application of rhGH might activate Mphi by IGF-1 induction, enhancing Mphi CD14 expression, which promoted more secretion of cytokines, and promote the enhancement of immune function.
Ben
D
D
Department of Burns, Changhai Hospital, The Second Military Medical University, Shanghai 200433 P.R. China.
Huan
J
J
Yang
L
L
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D019410:Interleukin-16 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D019410:Interleukin-16; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone