Composition of a chemopreventive proanthocyanidin-rich fraction from cranberry fruits responsible for the inhibition of 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity.
Phenolics from the American cranberry (Vaccinium macrocarpon) were fractionated into a series of proanthocyanidins and other flavonoid compounds by vacuum chromatography on a hydrophilic, porous polyvinylic gel permeation polymer. Antioxidant activity was not restricted to a particular class of components in the extract but was found in a wide range of the fractions. Significant chemopreventive activity, as indicated by an ornithine decarboxylase assay, was localized in one particular proanthocyanidin-rich fraction from the initial fractionation procedure. Further fractionation of the active anticarcinogenic fraction revealed the following components: seven flavonoids, mainly quercetin, myricetin, the corresponding 3-O-glycosides, (-)-epicatechin, (+)-catechin, and dimers of both gallocatechin and epigallocatechin types, and a series of oligomeric proanthocyanidins.
Kandil
Fayez E
FE
Department of Plant Biology, University of Illinois, Urbana, IL 61801, USA.
Smith
Mary Ann L
MA
Rogers
Randy B
RB
Pépin
Marie-France
MF
Song
Lynda L
LL
Pezzuto
John M
JM
Seigler
David S
DS
D000818:Animals; D000872:Anthocyanins / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D019281:Dimerization; D004817:Epidermis / Q000201:enzymology; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D010936:Plant Extracts / Q000737:chemistry*; D044945:Proanthocyanidins; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D029799:Vaccinium macrocarpon / Q000737:chemistry*
D000872:Anthocyanins; D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D065108:Ornithine Decarboxylase Inhibitors; D010936:Plant Extracts; D044945:Proanthocyanidins; C013221:proanthocyanidin; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate