title: Genomic organization of profilin-III and evidence for a transcript expressed exclusively in testis.
abstract: Profilins are small, widely expressed actin binding proteins, thought to be key regulators of actin dynamics in living cells. So far, three profilin-genes have been described: profilin-I (PFN1), profilin-II (PFN2) with two splice variants and the recently identified profilin-III (PFN3). Here we describe the genomic organization of the genes encoding human and mouse profilin-III. Both are single exon genes and lie in close vicinity to the renal sodium-phosphate transport gene 2 (SLC34A1, NPT2) which is highly expressed in kidney. Northern hybridization to rat tissues has previously demonstrated expression of an approximately 4.5 kb long profilin-III mRNA transcript in kidney and a mRNA transcript of approximately 1 kb in length in testis. Here we show that mouse profilin-III expression is restricted to testis and that the 4.2 kb profilin-III mRNA in kidney is the result of a slc34a1 transcript which includes the antisense profilin-III open reading frame in its 3'-untranslated region. Finally, we demonstrate by in situ hybridization that profilin-III mRNA is localized to cells in the late stage of spermatogenesis.
authors.affiliation: Department of Experimental Pathology, Lund University, S-22185, Lund, Sweden.
authors.forename: Attila
authors.initials: A
authors.lastname: Braun
authors.forename: Attila
authors.initials: A
authors.lastname: Aszódi
authors.forename: Heide
authors.initials: H
authors.lastname: Hellebrand
authors.forename: Alejandro
authors.initials: A
authors.lastname: Berna
authors.forename: Reinhard
authors.initials: R
authors.lastname: Fässler
authors.forename: Oliver
authors.initials: O
authors.lastname: Brandau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003285:Contractile Proteins; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D051304:Profilins; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D003285:Contractile Proteins; D018076:DNA, Complementary; D008840:Microfilament Proteins; C495738:PFN1 protein, human; C495741:PFN2 protein, human; C495739:Pfn1 protein, mouse; C495740:Pfn1 protein, rat; C495742:Pfn2 protein, mouse; C495743:Pfn2 protein, rat; C495744:Pfn3 protein, mouse; D051304:Profilins; D020033:Protein Isoforms; D012333:RNA, Messenger; D004247:DNA