title: Complement interaction with trypanosomatid promastigotes in normal human serum.
abstract: In normal human serum (NHS), axenic promastigotes of Crithidia, Phytomonas, and Leishmania trigger complement activation, and from 1.2 to 1.8 x 10(5) C3 molecules are deposited per promastigote within 2.5 min. In Leishmania, promastigote C3 binding capacity remains constant during in vitro metacyclogenesis. C3 deposition on promastigotes activated through the classical complement pathway reaches a 50% maximum after similar50 s, and represents >85% of total C3 bound. In C1q- and C2-deficient human sera, promastigotes cannot activate the classical pathway (CP) unless purified C1q or C2 factors, respectively, are supplemented, demonstrating a requirement for CP factor in promastigote C3 opsonization. NHS depleted of natural anti-Leishmania antibodies cannot trigger promastigote CP activation, but IgM addition restores C3 binding. Furthermore, Leishmania binds natural antibodies in ethylenediaminetetracetic acid (EDTA)-treated NHS; after EDTA removal, promastigote-bound IgM triggers C3 deposition in natural antibody-depleted NHS. Serum collectins and pentraxins thus do not participate significantly in NHS promastigote C3 opsonization. Real-time kinetic analysis of promastigote CP-mediated lysis indicates that between 85--95% of parasites are killed within 2.5 min of serum contact. These data indicate that successful Leishmania infection in man must immediately follow promastigote transmission, and that Leishmania evasion strategies are shaped by the selective pressure exerted by complement.
authors.affiliation: Servicio de Inmunología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, E-28220 Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Domínguez
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Moreno
authors.forename: Margarita
authors.initials: M
authors.lastname: López-Trascasa
authors.forename: Alfredo
authors.initials: A
authors.lastname: Toraño
mesh_terms: D000818:Animals; D003176:Complement C3 / Q000276:immunology*; D003171:Complement Pathway, Classical; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007700:Kinetics; D007891:Leishmania / Q000276:immunology*; D010587:Phagocytosis; D011485:Protein Binding; D014351:Trypanosomatina / Q000254:growth & development / Q000276:immunology*
chemical_list: D003176:Complement C3; D007075:Immunoglobulin M