Influence of BOL on hyaluronic acid, laminin and hyperplasia in hepatofibrotic rats.
OBJECTIVE
To study the anti-hepatofibrosis mechanism of Bie Jia Jian oral liquid (BOL).


METHODS
The model was induced by subcutaneous injection of CCl(4). BOL was administered and the change of serum hyaluronic acid (HA) and laminin (LN) was observed and the degeneration of liver cells and the degree of fibre hyperplasia analyzed. Changes of ultra micro-structure in liver cells were observed in some samples.


RESULTS
HA was reduced in both the groups with low and high dosage of BOL, which showed a remarkable difference as compared with that of the model group (low dosage group: 376.15 microg/L+/-35.48 microg/L vs 806.07 microg/L+/-98.49 microg/L P<0.05; high dosage group: 340.14 microg/L+/-30.18 microg/L vs 806.07 microg/L+/-98.49 microg/L P<0.05). The LN content of low and high dosage group of BOL was lower than that of model group (low dosage group: 71.99 microg/L+/-8.15 microg/L vs 133.94 microg/L+/-14.45 microg/L P <0.01; high dosage group: 71.68 microg/L+/-11.62 microg/L vs 133.94 microg/L+/-14.45 microg/L P<0.01) and colchicine group (low dosage group: 71.99 microg/L+/-8.15 microg/L vs 118.28 microg/L+/-16.13 microg/L P < 0.05; high dosage group: 71.68 microg/L+/-11.62 microg/L vs 118.28 microg/L+/-16.13 microg/L P <0.05). Examined by Ridit, BOL could reduce the degeneration and necrosis of liver cells (chi(2)=11.99 P<0.05), the degree of fibre hyperplasia (chi(2)=13.24 P<0.05) and the pathological change of ultra micro-structure as well.


CONCLUSIONS
The BOL has certain therapeutic effect on the experiment hepatofibrosis. Its mechanisms might include: protecting the function of liver cells, inhibiting excessive synthesis and secretion of extracellular matrix from hepatic stellate cells, relieving the capillarization of hepatic sinusoid, improving liver micro-circulation, and regulating immune function.
Yao
L
L
Pharmacology Department of Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, Zhejiang Province, China. ylyj@mail.hz.zj.cn
Yao
Z M
ZM
Yu
T
T
D000818:Animals; D006820:Hyaluronic Acid / Q000097:blood*; D006965:Hyperplasia; D007797:Laminin / Q000097:blood*; D008103:Liver Cirrhosis / Q000097:blood* / Q000473:pathology*; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley
D007797:Laminin; D006820:Hyaluronic Acid