title: Proline metabolism and NAD kinase activity in greenbean plants subjected to cold-shock.
abstract: The aim of the present work was to examine the relationship between proline metabolism and NAD kinase activity in greenbeans submitted to cold-shock. For this, 15-day-old greenbean plants were subjected to a temperature of 4 degrees C (cold shock) for 180 min. Our results indicate that the plants showed foliar accumulation of proline, with the enzymes ornithine-delta-aminotransferase (OAT) and proline dehydrogenase (PDH) appearing as determinant in this accumulation under cold-shock. Also, we found a close relationship between the Ca(2+)-CaM-dependent NAD kinase activity and proline metabolism, suggesting that the adaptive responses or acclimation of plants to cold stress are preceded by increased [Ca(2+)](cyt).
authors.affiliation: Department of Plant Biology, Faculty of Sciences, University of Granada, 18071, Granada, Spain. jruizsaez@ucdavis.edu
authors.forename: Juan M
authors.initials: JM
authors.lastname: Ruiz
authors.forename: Esteban
authors.initials: E
authors.lastname: Sánchez
authors.forename: Pablo C
authors.initials: PC
authors.lastname: García
authors.forename: Luis R
authors.initials: LR
authors.lastname: López-Lefebre
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Rivero
authors.forename: Luis
authors.initials: L
authors.lastname: Romero
mesh_terms: D003080:Cold Temperature; D007887:Fabaceae / Q000201:enzymology / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011392:Proline / Q000378:metabolism*
chemical_list: D011392:Proline; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase