title: A novel two-enzyme amperometric electrode for lactose determination.
abstract: Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.
authors.affiliation: Department of Chemistry, Suleyman Demirel University, Isparta, Turkey.
authors.forename: Handan
authors.initials: H
authors.lastname: Gülce
authors.forename: Ahmet
authors.initials: A
authors.lastname: Gülce
authors.forename: Attila
authors.initials: A
authors.lastname: Yildiz
mesh_terms: D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007785:Lactose / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011145:Polyvinyls; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D010710:Phosphates; D011145:Polyvinyls; C451099:polyvinylferrocenium; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D007785:Lactose