title: Interaction of PRMT1 with BTG/TOB proteins in cell signalling: molecular analysis and functional aspects.
abstract: BACKGROUND
  Several recent reports have connected protein methylation with differentiation. Furthermore, the BTG/TOB proteins have also been implicated in such control. BTG1 and 2 have been shown to interact with PRMT1 (predominant cellular arginine N-methyltransferase of type I).
  
  
  RESULTS
  First, we have studied the interaction between PRMT1 and the proteins of the BTG/TOB family. We show that boxC, a sequence present only in BTG1 and BTG2, is essential for this association. Using boxC peptide, we have investigated the importance of PRMT1/BTG protein association during type I protein methylation reactions. Finally, we show that the addition of boxC fused to penetratin interferes with the neuronal differentiation of PC12 cells and ES cell-derived neurones.
  
  
  CONCLUSIONS
  Taken together, these results indicate that PRMT1/BTG proteins could play a key role in the arginine methylation-mediated signalling pathway as well as in neuronal differentiation.
authors.affiliation: Unité INSERM U453, affiliée au CNRS, Centre Léon Bérard, Le Cheney, 28 rue Laënnec, 69373 Lyon Cedex 08, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Berthet
authors.forename: Fabienne
authors.initials: F
authors.lastname: Guéhenneux
authors.forename: Valérie
authors.initials: V
authors.lastname: Revol
authors.forename: Christiane
authors.initials: C
authors.lastname: Samarut
authors.forename: Agnès
authors.initials: A
authors.lastname: Lukaszewicz
authors.forename: Colette
authors.initials: C
authors.lastname: Dehay
authors.forename: Charles
authors.initials: C
authors.lastname: Dumontet
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Magaud
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Rouault
mesh_terms: D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D057846:Cell-Penetrating Peptides; D019175:DNA Methylation; D017874:Immediate-Early Proteins / Q000502:physiology*; D009363:Neoplasm Proteins / Q000502:physiology*; D009474:Neurons; D016716:PC12 Cells; D011484:Protein-Arginine N-Methyltransferases / Q000502:physiology*; D051381:Rats; D020539:Sequence Analysis, Protein; D015398:Signal Transduction; D013234:Stem Cells
chemical_list: C489553:Btg1 protein, rat; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D017874:Immediate-Early Proteins; D009363:Neoplasm Proteins; C414409:penetratin; D011484:Protein-Arginine N-Methyltransferases