Lack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.
Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.
Murphy
Mark
M
Division of Molecular Microbiology, Biozentrum of the University of Basel, CH-4056 Basel, Switzerland.
Schmid Nuoffer
Stefanie
S
Bickle
Thomas A
TA
D015075:2-Aminopurine / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D016296:Mutagenesis
D002135:Calcium-Binding Proteins; D029968:Escherichia coli Proteins; D015075:2-Aminopurine; D004262:DNA Restriction Enzymes; C059678:mcrB protein, E coli