Inhibition of heat shock-induced apoptosis by peppermint oil in astrocytes.
Exposure to environmental stresses and toxins is linked to the pathogenesis of neuropsychiatric disorders. Astrocytes, the most abundant glial-cell type in the brain, are considered to have physiological and pathological roles in neuronal activities. We have investigated whether peppermint oil inhibits heat shock-induced apoptosis of astrocytes. We found that peppermint oil inhibits the heat shock-induced apoptosis in both human astrocyte CCF-STTG1 cells and rat astrocytes. Pretreatment of the cells with peppermint oil inhibited the heat shock-induced DNA fragmentation and condensation of nuclear chromatin. Peppermint oil also inhibited the caspase-3 activation and poly-ADP-ribose polymerase fragmentation in CCF-STTG1 cells. These results suggest that peppermint oil may modulate the apoptosis of astrocytes via the activation of the caspase-3.
Koo
H N
HN
College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
Jeong
H J
HJ
Kim
C H
CH
Park
S T
ST
Lee
S J
SJ
Seong
K K
KK
Lee
S K
SK
Lyu
Y S
YS
Kim
H M
HM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D053938:DNA Fragmentation / Q000187:drug effects; D004586:Electrophoresis; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D036142:Mentha piperita; D010938:Plant Oils / Q000494:pharmacology*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D051381:Rats
D010938:Plant Oils; D011506:Proteins; C015424:peppermint oil; C499320:PARP1 protein, human; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases