title: Developmentally programmed excision of internal DNA sequences in Paramecium aurelia.
abstract: The development of a new somatic nucleus (macronucleus) during sexual reproduction of the ciliate Paramecium aurelia involves reproducible chromosomal rearrangements that affect the entire germline genome. Macronuclear development can be induced experimentally, which makes P. aurelia an attractive model for the study of the mechanism and the regulation of DNA rearrangements. Two major types of rearrangements have been identified: the fragmentation of the germline chromosomes, followed by the formation of the new macronuclear chromosome ends in association with imprecise DNA elimination, and the precise excision of internal eliminated sequences (IESs). All IESs identified so far are short, A/T rich and non-coding elements. They are flanked by a direct repeat of a 5'-TA-3' dinucleotide, a single copy of which remains at the macronuclear junction after excision. The number of these single-copy sequences has been estimated to be around 60,000 per haploid genome. This review focuses on the current knowledge about the genetic and epigenetic determinants of IES elimination in P. aurelia, the analysis of excision products, and the tightly regulated timing of excision throughout macronuclear development. Several models for the molecular mechanism of IES excision will be discussed in relation to those proposed for DNA elimination in other ciliates.
authors.affiliation: Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 46, rue d'Ulm, 75005 Paris, France. gratias@wotan.ens.fr
authors.forename: A
authors.initials: A
authors.lastname: Gratias
authors.forename: M
authors.initials: M
authors.lastname: Bétermier
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D002875:Chromosomes; D053938:DNA Fragmentation; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D015321:Gene Rearrangement; D008957:Models, Genetic; D010247:Paramecium / Q000235:genetics* / Q000254:growth & development
chemical_list: D016054:DNA, Protozoan