An NMR approach to structural proteomics.
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
Yee
Adelinda
A
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, ON, Canada M5G 1L7.
Chang
Xiaoqing
X
Pineda-Lucena
Antonio
A
Wu
Bin
B
Semesi
Anthony
A
Le
Brian
B
Ramelot
Theresa
T
Lee
Gregory M
GM
Bhattacharyya
Sudeepa
S
Gutierrez
Pablo
P
Denisov
Aleksej
A
Lee
Chang-Hun
CH
Cort
John R
JR
Kozlov
Guennadi
G
Liao
Jack
J
Finak
Grzegorz
G
Chen
Limin
L
Wishart
David
D
Lee
Weontae
W
McIntosh
Lawrence P
LP
Gehring
Kalle
K
Kennedy
Michael A
MA
Edwards
Aled M
AM
Arrowsmith
Cheryl H
CH
D001426:Bacterial Proteins / Q000737:chemistry; D019992:Databases as Topic; D004926:Escherichia coli / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D016366:Open Reading Frames; D011506:Proteins / Q000737:chemistry*; D012984:Software; D013045:Species Specificity
D001426:Bacterial Proteins; D011506:Proteins