Novel approaches for identifying genes regulating lymphocyte development and function.
The draft sequence of the human and mouse genomes provides an unparalleled opportunity for understanding the genetic control of immune-cell development. Strategies can begin with a gene sequence and pursue a putative immune-system function by employing mRNA-expression profiling or creating gene knockouts in embryonic stem cells. The latter can be produced by utilising the Cre/Lox system, a tetracycline operon, a gene-trap method or chemical mutagenesis. Alternatively, mutant phenotypes (derived using the mutagen ethylnitrosourea) can be traced back to gene sequences.
Loy
Adèle L
AL
Australian Cancer Research Foundation (ACRF) Genetics Laboratory and Medical Genome Centre, John Curtin School of Medical Research, Australian National University, Canberra, Australia. adele.loy@anu.edu.au
Goodnow
Christopher C
CC
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics* / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D016678:Genome; D006801:Humans; D007107:Immune System / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D051379:Mice