A novel two-enzyme amperometric electrode for lactose determination.
Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.
Gülce
Handan
H
Department of Chemistry, Suleyman Demirel University, Isparta, Turkey.
Gülce
Ahmet
A
Yildiz
Attila
A
D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D005949:Glucose Oxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007785:Lactose / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D011145:Polyvinyls; D001616:beta-Galactosidase / Q000737:chemistry*
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D010710:Phosphates; D011145:Polyvinyls; C451099:polyvinylferrocenium; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D001616:beta-Galactosidase; D007785:Lactose