Modification of capillary electrophoresis capillaries by poly(hydroxyethyl methacrylate), poly(diethylene glycol monomethacrylate) and poly(triethylene glycol monomethacrylate).
Modification of capillary electrophoresis (CE) capillaries by poly(hydroxyethyl methacrylate) (poly(HEMA), poly(diethylene glycol monomethacrylate) (poly(DEGMA) and poly(triethylene glycol monomethacrylate) (poly(TEGMA), was studied. Methods based on physical adsorption of the modifier and on its chemical binding were compared on the basis of the electroosmotic flow (EOF) reproducibility, the EOF dependence on the pH, the symmetry of the peak of positively charged tyramine, the stability of the coating and the separation of standard and milk proteins in the modified capillaries. Reproducible coatings were obtained by chemical binding of the polymers to the capillary walls and by coating with a solution of a polymer, as also demonstrated by the atomic force microscopy.
Strelec
Ivo
I
Charles University, Department of Analytical Chemistry, Prague, Czech Republic.
Pacáková
Vera
V
Bosáková
Zuzana
Z
Coufal
Pavel
P
Guryca
Vilém
V
Stulík
Karel
K
D000327:Adsorption; D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000493:pharmacokinetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D057927:Hydrophobic and Hydrophilic Interactions; D008894:Milk Proteins / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000493:pharmacokinetics; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry* / Q000493:pharmacokinetics; D011109:Polymethacrylic Acids / Q000737:chemistry* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D008894:Milk Proteins; D011109:Polymethacrylic Acids; C466018:poly(triethylene glycol monomethacrylate); D011102:Polyhydroxyethyl Methacrylate; D011092:Polyethylene Glycols