title: Molecular characterization of Candida spp. isolated from the oral cavities of patients from diverse clinical settings.
abstract: Infections by Candida spp. have increased in medical importance over the past few decades. Our understanding of species identification, commensalisms, pathogenicity, person-to-person spread, and the development of antifungal resistance within specific strains has been greatly enhanced by the utilization of molecular epidemiological methodology. The aim of the current research was to assess the quantity, species and molecular characterization of oral yeast isolates from well-defined cohorts of immunocompetent patients from a diverse range of clinical settings. Oral rinse samples were assessed for the growth of yeast and degree of colonization. Isolates were defined to the species level by both phenotypic and molecular methods and strains were further genotypically subtyped. Significant variation was shown to exist in the number, species and genotypic subgroups of yeast isolated from the oral cavity in different patient groups. This variation could be attributed to the local oral conditions unique to these patient groups.
authors.affiliation: Oral Medicine Department, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Grays Inn Road, London WC1 9LD, UK.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Al-Karaawi
authors.forename: M
authors.initials: M
authors.lastname: Manfredi
authors.forename: A C W
authors.initials: AC
authors.lastname: Waugh
authors.forename: Michael J
authors.initials: MJ
authors.lastname: McCullough
authors.forename: J
authors.initials: J
authors.lastname: Jorge
authors.forename: C
authors.initials: C
authors.lastname: Scully
authors.forename: S R
authors.initials: SR
authors.lastname: Porter
mesh_terms: D000328:Adult; D000368:Aged; D002175:Candida / Q000145:classification* / Q000235:genetics*; D002180:Candidiasis, Oral / Q000453:epidemiology; D016009:Chi-Square Distribution; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D009061:Mouth Mucosa / Q000382:microbiology*; D016533:Mycological Typing Techniques
chemical_list: D004271:DNA, Fungal