Selective binding of albumin on stearyl poly(ethylene oxide) coupling polymer-modified poly(ether urethane) surfaces.
A tri-block-coupling polymer of stearyl poly(ethylene oxide)-4,4'-methylene diphenyl diisocyanate-stearyl poly(ethylene oxide) (MSPEO), was used as a surface modifying additive (SMA) and the MSPEO-modified poly(ether urethane) (PEU) surfaces were prepared by the process of dip-coating. The surface analysis by XPS revealed the surface enrichment of poly(ethylene oxide) (PEO). On the coating-modified surfaces, the bovine serum albumin (BSA) adsorption, respectively, from the low and high BSA bulk concentration solutions was correspondingly characterized by the methods of radioactive 125I-probe and ATR-FTIR. The bovine serum fibrinogen (Fg)-adsorption from the Fg bulk solution and the BSA-Fg competing adsorption from the BSA-Fg binary solutions were also characterized by radioactive 125I-probe. The reversible BSA-selective in situ adsorption on MSPEO-modified PEU surfaces were achieved, and the performance of blood compatibility on the coating-modified surfaces was also confirmed, respectively, by plasma recalcification time (PRT) and prothrombin time (PT) tests.
Wang
D A
DA
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, PR China. Jianji@mail.hz.zj.cn
Ji
J
J
Feng
L X
LX
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001769:Blood; D002417:Cattle; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008422:Materials Testing; D011092:Polyethylene Glycols; D011140:Polyurethanes; D011485:Protein Binding; D011517:Prothrombin Time; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013228:Stearates; D013499:Surface Properties
D001672:Biocompatible Materials; D007457:Iodine Radioisotopes; D011140:Polyurethanes; D013228:Stearates; C115016:polyetherurethane; C439572:stearyl poly(ethylene oxide); D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols