[The adhesive and migrating function of human epithelial cells opsonized by fibronectin].
OBJECTIVE
To investigate the adhesive and migrating function of human epithelial keratiocytes during in vitro culture.


METHODS
The adhesive and migrating function was determined in freshly isolated EKCs and those cultured for 7 similar 10 days after being opsonized by fibronectin (FN). The expressions of alpha(5)beta(1) receptors in EKC were examined with indirect immunofluorescence staining methods before and after culture.


RESULTS
(1) The adhesive and migrating indices after FN opsonization of EKCs freshly isolated EKCs were obviously lower than those cultured for 7 similar 10 days (P < 0.01). (2) There exhibited positive staining of the expression of alpha(5)beta(1) receptors in the EKC after 1 day culture and in the proliferative epithelia after in vitro culturing of a tissue mass. While there exhibited strongly positive staining of the peripheral proliferative epithelia of the EKCs and tissue mass after 7 days of culture, negative or weakly positive stainings were found in freshly isolated EKCs.


CONCLUSIONS
(1) There existed significant difference of biological function between the freshly isolated EKCs and those cultured for 7 similar 10 days. (2) The strongest expression of alpha(5)beta(1) receptors was observed at the active proliferative site (peripheral proliferative epithelia of a tissue mass) and in the biological active period (cultured for 7 days) of EKCs. (3) The adhesive and migrating function of EKCs could be effectively induced and activated by in vitro culture, which enabled the EKC to alter from a relatively biologically functional static state to an actively functional state.
Cao
Q
Q
Municipal Institute of Burn Research of Shanghai, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200025, P. R. China.
Xu
W
W
Jin
S
S
D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017469:Receptors, Fibronectin / Q000096:biosynthesis
D005353:Fibronectins; D017469:Receptors, Fibronectin