Detection of the gp43 gene and (1-3)-beta-D-glucan of Paracoccidioides brasiliensis in the blood of experimentally infected mice.
Paracoccidioidomycosis (PCM) is a deep mycosis caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. Diagnosis of PCM is sometimes difficult outside the endemic countries, thus a rapid and conclusive method for diagnosis of PCM has been anticipated. We compared the sensitivities of a nested PCR method for detecting the gp43 gene and a commercial kit for detecting (1-3)-beta-D-glucan in the blood of experimentally infected mice. Blood samples were collected from mice at 0 (soon after inoculation), 6, 12, 24, 48, and 72 hours and 5, 7, 10, 14, 17, 21, 24, 28 and 56 days after the intravenous inoculation of 10(6) yeast cells of P. brasiliensis, and were separated into clots and plasma. The (1-3)-beta-D-glucan detection kit in the plasma showed positive reactions in some samples within 7 days and 28 and 56 days after infections. In contrast, the PCR method was more sensitive than the (1-3)-beta-D-glucan detection kit throughout the observation period. The clot samples yielded more sensitive PCR-results than did the plasma samples. Although 24 hours is required for the PCR detection, it was confirmed to provide an accurate diagnosis of PCM.
Nakagawa Itano
Eiko
E
Universidade Estadual de Londrina, CCB-PAT-Campus Universitario, CEP: 86051-970, Londrina, Parana, Brazil.
Uno
Jun
J
Sano
Ayako
A
Yarita
Kyoko
K
Kamei
Katsuhiko
K
Miyaji
Makoto
M
Nishimura
Kazuko
K
Mikami
Yuzuru
Y
D000328:Adult; D000368:Aged; D000818:Animals; D000946:Antigens, Fungal; D015415:Biomarkers / Q000097:blood; D002648:Child; D011756:Diphosphates; D004195:Disease Models, Animal; D004338:Drug Combinations; D005260:Female; D005656:Fungal Proteins; D005800:Genes, Fungal; D005936:Glucans / Q000097:blood*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008875:Middle Aged; D009566:Nitrates; D010228:Paracoccidioides / Q000235:genetics*; D010229:Paracoccidioidomycosis / Q000175:diagnosis*; D011095:Polyethylenes; D016133:Polymerase Chain Reaction / Q000379:methods; D012969:Sodium Fluoride; D047071:beta-Glucans
C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D015415:Biomarkers; D011756:Diphosphates; D004338:Drug Combinations; D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; D009566:Nitrates; D011095:Polyethylenes; C097426:Sensitive Tartar Control Dentifrice; D047071:beta-Glucans; D012969:Sodium Fluoride; C033363:beta-1,3-glucan