Brain-derived neurotrophic factor and nitric oxide synthase inhibitor protect the vestibular organ against gentamicin ototoxicity.
In order to find a way to develop a new treatment for inner ear disorders, the effects of a nitric oxide synthase (NOS) inhibitor [N-nitro-L-arginine methylester (L-NAME)] and a neurotrophin [brain-derived neurotrophic factor (BDNF)] were investigated. The effect of L-NAME and BDNF on gentamicin-induced vestibular hair cell damage was investigated by using the in vitro LIVE/DEAD system. Both L-NAME and BDNF individually reduced the vestibular hair cell damage caused by gentamicin but the combination of L-NAME and BDNF was more successful in preventing damage. It is therefore suggested that treatment with a combination of an NOS inhibitor and a neurotrophin will help us to treat inner ear disorders.
Takumida
Masaya
M
Department of Otolaryngology, Hiroshima University Faculty of Medicine, Japan. masati@hiroshima-u.ac.jp
Anniko
Matti
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D014722:Vestibule, Labyrinth / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D019208:Brain-Derived Neurotrophic Factor; D004791:Enzyme Inhibitors; D005839:Gentamicins; D019331:NG-Nitroarginine Methyl Ester