title: Chemokine and cytokine expression in murine intestinal epithelium following Nippostrongylus brasiliensis infection.
abstract: Infection of mice with the nematode parasite Nippostrongylus brasiliensis results in a well characterized intestinal mastocytosis with intraepithelial migration of mucosal mast cells (MMC). The molecules mediating this response are unknown. We examined expression of several putative mast cell chemoattractants in intestinal epithelium following N. brasiliensis infection. Expression of the chemokines monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1alpha), RANTES (regulated on activation normal T-cell expressed and secreted), fractalkine, and thymocyte expressed chemokine (TECK); and the cytokines stem cell factor (SCF) and transforming growth factor beta1 (TGFbeta1), was constitutive and no alteration was detected following infection. MCP-1 expression was also constitutive but at much lower levels and increased expression was detected on days 7 and 14 postinfection. Expression of MCP-1 in whole jejunum was at much higher levels than in epithelium. Constitutive expression of MCP-1, MIP-1alpha and TGFbeta1 was also detected in cultured bone marrow-derived homologues of MMC. In an intestinal epithelial cell line (CMT-93), there was constitutive expression of SCF, TGFalpha1, fractalkine and MCP-1. The results show that, in vivo, epithelium is a potentially important source of mast cell chemoattractants.
authors.affiliation: Department of Veterinary Pathology, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Rosbottom
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Knight
authors.forename: Gerry
authors.initials: G
authors.lastname: McLachlan
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Thornton
authors.forename: Steven W
authors.initials: SW
authors.lastname: Wright
authors.forename: Hugh R P
authors.initials: HR
authors.lastname: Miller
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Scudamore
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology / Q000648:ultrastructure; D007583:Jejunum / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009559:Nippostrongylus / Q000502:physiology*; D010270:Parasite Egg Count; D017385:Sequence Homology; D017206:Strongylida Infections / Q000276:immunology* / Q000469:parasitology / Q000473:pathology
chemical_list: D018925:Chemokines; D016207:Cytokines