Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage-gated K(+) channel Kv2.1.
We report identification and characterization of Kv6.3, a novel member of the voltage-gated K(+) channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K(+) channel which functions as a modulatory subunit.
Sano
Yorikata
Y
Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Ibaraki 305-8585, Tsukuba, Japan. sano.yorikata@yamanouchi.co.jp
Mochizuki
Shinobu
S
Miyake
Akira
A
Kitada
Chika
C
Inamura
Kohei
K
Yokoi
Hiromichi
H
Nozawa
Katsura
K
Matsushime
Hitoshi
H
Furuichi
Kiyoshi
K
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D051668:Delayed Rectifier Potassium Channels; D004553:Electric Conductivity; D006801:Humans; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D017386:Sequence Homology, Amino Acid; D051669:Shab Potassium Channels; D014018:Tissue Distribution
D051668:Delayed Rectifier Potassium Channels; C496988:KCNB1 protein, human; C450424:KCNG3 protein, human; C499182:Kcng3 protein, rat; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D021122:Protein Subunits; D051669:Shab Potassium Channels