Sharpening spots: correcting for bleedover in cDNA array images.
For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.
Therneau
Terry
T
Department of Health Sciences Research, Mayo Foundation, Rochester, MN 55905, USA. therneau.terry@mayo.edu
Tschumper
Renee C
RC
Jelinek
Diane
D
D000465:Algorithms; D018076:DNA, Complementary / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D009101:Multiple Myeloma / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods* / Q000592:standards
D018076:DNA, Complementary