rpoB genotypes of Mycobacterium tuberculosis Beijing family isolates from East Asian countries.
The 81-bp region of the rpoB gene in 66 Rif(r) Mycobacterium tuberculosis isolates from China, Japan, Korea, and Taiwan was analyzed. Twelve single-nucleotide substitutions in the rpoB gene were detected. The most prevalent mutations were at Ser-531 (52%), Asp-516 (17%), and His-526 (11%). Mutations were not found in seven (11%) of the isolates. Higher mutation rates in 50 Beijing family isolates were found than in other isolates for mutations at Asp-516 (18 and 12.5%, respectively) and His-526 (12 and 6.3%, respectively). The different rates of mutation may reflect the choice of rifamycin analogs.
Qian
Lishi
L
Department of Microbiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA.
Abe
Chiyoji
C
Lin
Tao-Ping
TP
Yu
Ming-Chih
MC
Cho
Sang-Nae
SN
Wang
Sumin
S
Douglas
James T
JT
D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D005838:Genotype; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin