New lupane glycosides from Pulsatilla chinensis.
Two new lupane glycosides along with five known triterpenoids were isolated from the roots of Pulsatilla chinensis (Ranunculaceae). The structures of the new glycosides were determined to be 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside D, 6) and 3-O-[beta-D-glucopyranosyl(1-->4)][alpha-L-rhamnopyranosyl(1-->2)]-alpha-L-arabinopyranosyl-23-hydroxybetulinic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glucopyranosyl(1-->6)-beta-D-glucopyranosyl ester (pulsatilloside E, 7) by spectroscopic analysis and chemical methods. The compounds were evaluated for cytotoxic activities against K-562 human leukemia and HeLa cells.
Ye
Wencai
W
Zhang
Qingwen
Q
Hsiao
Wendy W
WW
Zhao
Shouxun
S
Che
Chun-Tao
CT
D001681:Biological Assay; D002240:Carbohydrate Sequence; D002681:China; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D029626:Ranunculaceae; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes; D000094062:Betulinic Acid
D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C455124:pulsatilloside D; D000094062:Betulinic Acid; C455125:pulsatilloside E