title: Lipopolysaccharide-specific but not anti-flagellar immunoglobulin A monoclonal antibodies prevent Salmonella enterica serotype enteritidis invasion and replication within HEp-2 cell monolayers.
abstract: The protective potential of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis to prevent bacterial adhesion to and invasion of HEp-2 cells was evaluated. Although anti-flagellar IgA MAbs showed strong agglutinating capacities, they did not protect cell monolayers. In contrast, IgA MAbs specific for the O:9 epitope of Salmonella lipopolysaccharide antigen alone prevented S. enterica serotype Enteritidis entry and replication within HEp-2 cells, and the protection was not mediated by direct binding of antibodies to bacterial adhesins or by agglutination of microorganisms.
authors.affiliation: Department of Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria.
authors.forename: Ianko D
authors.initials: ID
authors.lastname: Iankov
authors.forename: Dragomir P
authors.initials: DP
authors.lastname: Petrov
authors.forename: Ivan V
authors.initials: IV
authors.lastname: Mladenov
authors.forename: Iana H
authors.initials: IH
authors.lastname: Haralambieva
authors.forename: Ivan G
authors.initials: IG
authors.lastname: Mitov
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000918:Antibody Specificity; D001422:Bacterial Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005407:Flagella / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology*; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D008070:Lipopolysaccharides