Atrophic change of rat salivary gland during adenovirus-induced hyperleptinemia.
Sustained hyperleptinemia in normal rats induced by infusing a recombinant adenovirus containing the rat leptin cDNA (AdCMV-leptin) exhibited a remarkable reduction in food intake (AdCMV-leptin, 9.3 +/- 2.6 vs untreated, 20.6 +/- 1.0 g/day) and ablated body fat without any significant changes in wet weight of liver and left ventricle. In those hyperleptinemic rats, we found a 52% reduction in wet weight of salivary gland compared with that in the pair-fed AdCMV-beta-gal-treated rats, which received a recombinant virus containing the beta-galactosidase gene (AdCMV-beta-gal) and were fed on the same amount of food as had been consumed by the AdCMV-leptin-treated group on the previous day. Microscopic examination with hematoxylin-eosin staining revealed that atrophic change was induced in both serous and mucous gland only in the AdCMV-leptin-treated group, but not in the pair-fed controls. Thus, the atrophic changes in hyperleptinemic rats were due to neither a decrease of food intake nor disuse of the salivary gland related with anorexia. Our data suggested that size of the salivary gland was controlled, at lease in part, by "non-anorexic" effect of leptin.
Higa
Moritake
M
Second Department of Internal Medicine, University of the Ryukyus, Okinawa, 903-0215, Japan. hiji85f@ryukyu.ne.jp
Shimabukuro
Michio
M
Fukuchi
Mari
M
Komiya
Ichiro
I
Takasu
Nobuyuki
N
D000256:Adenoviridae / Q000235:genetics*; D000273:Adipose Tissue / Q000473:pathology; D000818:Animals; D001284:Atrophy / Q000473:pathology; D004435:Eating; D004822:Epididymis / Q000473:pathology; D005822:Genetic Vectors; D006352:Heart Ventricles / Q000473:pathology; D007700:Kinetics; D020738:Leptin / Q000097:blood / Q000235:genetics*; D008099:Liver / Q000473:pathology; D008297:Male; D009929:Organ Size; D051381:Rats; D011924:Rats, Zucker; D012466:Salivary Gland Diseases / Q000097:blood / Q000209:etiology* / Q000473:pathology; D012469:Salivary Glands / Q000473:pathology*
D020738:Leptin