Defective proliferative responses in B lymphocytes and thymocytes that lack neurofibromin.
Nf1(-/-) fetal liver cells were used to reconstitute B and T cells in Rag-1(-/-) mice. Lymphocyte development was largely unimpaired in the absence of neurofibromin. However antigen-receptor induced proliferation was defective in neurofibromin deficient peripheral B cells and CD4(+) single positive thymocytes. In contrast to its role as a negative regulator of proliferation in many other cell types, neurofibromin may be a positive regulator of lymphocyte proliferation. Peripheral B cells exhibited circumscribed defects in anti-IgM induced protein tyrosine phosphorylation, which may contribute to the unexpected proliferative defect seen in these cells.
Kim
Tae Jin
TJ
Cancer Center, Massachusetts General Hospital, Building 149, 13th Street, Charlestown Navy Yard, Boston, MA 02129, USA.
Cariappa
Annaiah
A
Iacomini
John
J
Tang
Mei
M
Shih
Shane
S
Bernards
Andre
A
Jacks
Tyler
T
Pillai
Shiv
S
D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D017353:Gene Deletion; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D025542:Neurofibromin 1 / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology
D025542:Neurofibromin 1