Role of integrins and intracellular adhesion molecule-1 in lung injury after intestinal ischemia-reperfusion.
BACKGROUND
We tested the hypothesis that lung injury after intestinal ischemia-reperfusion (IR) requires the activation of CD11/CD18 glycoprotein complex and its ligand, intracellular adhesion molecule-1 (ICAM-1), on pulmonary endothelial surface.


METHODS
Rats were assigned to one of six groups including sham operation, intestinal IR (60/120 min) and IR plus treatment with one of the following monoclonal antibodies against CD11a, CD11b, CD18, and ICAM-1. Pulmonary microvascular permeability, neutrophil accumulation, and expression of adhesion molecules were evaluated.


RESULTS
Intestinal IR resulted in lung injury characterized by a marked increase in microvascular permeability, neutrophil accumulation and upregulated expression of leukocyte integrins and ICAM-1. The increase in pulmonary microvascular permability and neutrophil accumulation elicited by intestinal reperfusion was effectively prevented by administration of blocking antibodies against ICAM-1, CD11, and CD18.


CONCLUSIONS
These results indicate that adhesion molecules contribute to the lung injury after intestinal IR. Immunoneutralization of certain of these adhesion molecules may prevent intestinal IR-induced lung injury.
Kuzu
M Ayhan
MA
Department of Surgery, Ankara University Medical School, Ivedik Caddesi 81/32 Demetevler, 06200 Ankara, Turkey.
Köksoy
Cüneyt
C
Kuzu
Işinsu
I
Gürhan
Ismet
I
Ergün
Hakan
H
Demirpence
Ediz
E
D000818:Animals; D002199:Capillary Permeability; D016023:Integrins / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007422:Intestines / Q000098:blood supply*; D008024:Ligands; D008168:Lung / Q000098:blood supply / Q000473:pathology*; D008297:Male; D008833:Microcirculation; D009504:Neutrophils; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology*; D015854:Up-Regulation
D016023:Integrins; D008024:Ligands; D018799:Intercellular Adhesion Molecule-1