[Evidence for TCR Vbeta clonal expansion T cells in a patient with cGVHD after unrelated donor BMT].
OBJECTIVE
To investigate the distribution and clonal expansion of TCR Vbeta subfamily T cells in patients with cGVHD after unrelated donor bone marrow transplantation (BMT).


METHODS
The CDR3 of 24 TCR Vbeta subfamily genes were amplified from peripheral mononuclear cells of a patient with cGVHD after unrelated BMT. RT-PCR was used for detection of the distribution of TCR Vbeta repertoire, and the PCR products were further analyzed by genescan technique for the CDR3 size, to evaluating clonality of the detectable TCR VbetaT cells.


RESULTS
Only 8 Vbeta subfamily T cells could be identified in the patient, clonal expansion T cells could be found of Vbeta2, 3, 8 and 13 subfamilies.


CONCLUSIONS
The skew distribution and clonal expansion of TCR Vbeta subfamily T cells could be found in the patient. It may relate to the initiation of cGVHD.
Du
X
X
Department of Hematology, Guangdong provincial People's Hospital, Guangzhou 510080, China.
Li
Y
Y
Weng
J
J
Lu
Z
Z
Su
J
J
Lin
W
W
Huang
Z
Z
D000208:Acute Disease; D000293:Adolescent; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology / Q000235:genetics*; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000628:therapy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D022801:Complementarity Determining Regions; D018076:DNA, Complementary; D016693:Receptors, Antigen, T-Cell, alpha-beta