Expression of a decorin-like moleculein human melanoma.
Decorin, a member of the family of small leucin-rich proteoglycans, has originally been described as a secreted proteoglycan component of the connective tissues, and has been implicated in the negative regulation of cell proliferation directly or via interactions with TGF-beta. It was reported to be generally absent from tumor cells. Here we show that human melanoma cell lines express a decorin-like molecule. We detected decorin mRNA by RT-PCR in 7 out 7 human melanoma lines characterized by various metastatic potential. Using polyclonal antiserum against the core protein of decorin, the typical 80-120 kD glycanated form as well as a high molecular weight aberrant version (200-210 kD) of decorin were demonstrated by Western blot technique in the culture supernatants as well as in lysates of human melanoma cells. Finally, decorin epitope was also demonstrated immunohistochemically in human melanoma xenografts, as well as in tumor cells of surgically resected melanomas but not in melanocytes of nevi. The expression of this aberrant decorin did not inhibit the in vitroor in vivogrowth of human melanoma cells, and it was independent of their metastatic potential. Human melanoma cell lines expressing aberrant decorin retained sensitivity to the antiproliferative and gelatinase-stimulatory effects of exogenous TGF-beta.
Ladányi
A
A
National Institute of Oncology, Department of Tumor Progression Ráth Gy. u.7-9., Budapest, H-1122, Hungary.
Gallai
M
M
Paku
S
S
Nagy
J O
JO
Dudás
J
J
Tímár
J
J
Kovalszky
I
I
D000818:Animals; D015139:Blotting, Southern; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D017364:Collagenases / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000166:cytology / Q000187:drug effects
C546878:DCN protein, human; D017931:DNA Primers; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D017364:Collagenases