title: [Analysis of TCR-gamma gene rearrangement in single H/R-S cells of Hodgkin disease].
abstract: OBJECTIVE
  To detect the rearrangement of T cell receptor gamma chain gene in single H/R-S cell as an indication of early T lymphocyte differentiation.
  
  
  METHODS
  CD30 positive H/R-S cells were isolated with micromanipulator from the frozen sections and analyzed by single-cell PCR. TCR-gamma chain gene rearrangement was detected through semi-nested PCR. In the first round of PCR, a pair of TCR-gamma primers which amplify the segments between Vgamma and Jgamma region (including the N region) of TCR-gamma gene mixing with a pair of beta-globin primers were used. In the second round, only the products positive beta-globin were amplified with the primers for TCR-gamma gene.
  
  
  RESULTS
  A 268 bp beta-globin gene specific product was obtained in 24 out of 56 H/R-S cells from 6 cases of Hodgkin's disease. After the first round of PCR, no rearrangement of the TCR-gamma gene was noticed at a single cell level.
  
  
  CONCLUSIONS
  H/R-S cells from the selected cases were not derived from the T-cell lineage but possibly from the B-cell lineage.
authors.affiliation: Department of Pathology, The First University Hospital of West China University of Medical Sciences, Chengdu 610041, China.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000473:pathology; D002648:Child; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D019675:Genes, T-Cell Receptor gamma; D006689:Hodgkin Disease / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D006801:Humans; D017730:Ki-1 Antigen / Q000032:analysis; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000473:pathology*
chemical_list: D017730:Ki-1 Antigen