title: Interaction of fibronectin and aggregation substance promotes adherence of Enterococcus faecalis to human colon.
abstract: This in vitro study investigates the interaction between aggregation substance (AS), a virulence factor of Enterococcus faecalis, and colonic mucosal fibronectin in normal colon and colon from patients with Crohn's disease. Fibronectin was found to be overexpressed in Crohn's disease compared to normal colon. Compared to E. faecalis OG1X:pAM944 (AS-negative), E. faecalis OG1X:pAM721 (expressing AS) showed a significantly enhanced adhesion to human colonic mucosa in normal colon and in colon from patients with Crohn's disease. Double-staining of fibronectin and AS-positive enterococci showed that colocalization of bacteria and fibronectin was significantly more frequent in Crohn's disease than in normal colon. Preincubation of bacteria with soluble fibronectin caused a significant reduction in the adherence to fibronectin. In conclusion, the interaction between AS and fibronectin plays is an important factor that mediates adhesion of Enterococcus faecalis to colonic mucosa. This might be one of the mechanisms responsible for bacterial translocation of Enterococcus faecalis.
authors.affiliation: Department of General Surgery, University of Ulm, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Isenmann
authors.forename: M
authors.initials: M
authors.lastname: Schwarz
authors.forename: E
authors.initials: E
authors.lastname: Rozdzinski
authors.forename: C
authors.initials: C
authors.lastname: Christ
authors.forename: E
authors.initials: E
authors.lastname: Schmidt
authors.forename: P
authors.initials: P
authors.lastname: Augat
authors.forename: R
authors.initials: R
authors.lastname: Marre
authors.forename: H G
authors.initials: HG
authors.lastname: Beger
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000494:pharmacology*; D018988:Bacterial Translocation; D003106:Colon / Q000382:microbiology*; D003424:Crohn Disease / Q000378:metabolism / Q000382:microbiology; D013293:Enterococcus faecalis / Q000472:pathogenicity*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D001426:Bacterial Proteins; D005353:Fibronectins; C065937:aggregation substance, Enterococcus faecalis