Immuno-capture PCR for detection of Aeromonas hydrophila.
In this report, we describe the use of universal primer PCR (UPPCR) for the detection of 16S ribosomal RNA (rRNA) genes from Aeromonas hydrophila captured by anti-A. hydrophila antibody coupled to a microplate. The approach combining immuno-capture with UPPCR provides a quick, sensitive, and reproducible way for the detection of bacterial cells.
Peng
Xuanxian
X
Department of Biology and the Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, PR China. wangpeng@xmu.edu.cn
Zhang
Jianying
J
Wang
Sanying
S
Lin
Zhilin
Z
Zhang
Weiyi
W
D016980:Aeromonas hydrophila / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000907:Antibodies, Bacterial; D017931:DNA Primers; D007118:Immunoassay; D016133:Polymerase Chain Reaction / Q000379:methods*
D000907:Antibodies, Bacterial; D017931:DNA Primers