Pulmonary carbonic anhydrase in the garter snake, Thamnophis sirtalis.
This study examined whether the snake lung possesses intravascular carbonic anhydrase (CA). Lungs were perfused with control salines and with salines containing CA inhibitors. Perfusion with control salines resulted in a stable CO(2) excretion, whereas CA inhibitors significantly reduced pulmonary CO(2) excretion. Membrane-permeable and membrane-impermeable CA inhibitors produced comparable decreases in CO(2) excretion, suggesting that extracellular, intravascular CA participated in the pulmonary CO(2)-HCO3(-)-H(+) reactions. Treatment of lungs with phosphatidylinositol specific-phospholipase C (PI-PLC) significantly decreased CO(2) excretion, indicating that CA was connected to the luminal endothelial cell membrane by a phosphatidylinositol glycan linkage. Taken together, these results are the first to demonstrate the presence of membrane-bound, intravascular CA (CA IV) in the snake lung.
Stabenau
Erich K
EK
Department of Biology, Bradley University, 1501 W. Bradley Avenue, Peoria, IL 61625, USA. eks@bradley.edu
Vietti
Kimberly R N
KR
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism / Q000502:physiology*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000302:isolation & purification / Q000502:physiology; D017812:Colubridae / Q000378:metabolism / Q000502:physiology*; D005110:Extracellular Space / Q000201:enzymology / Q000502:physiology; D008168:Lung / Q000201:enzymology*; D010477:Perfusion / Q000379:methods; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D013990:Tidal Volume / Q000502:physiology*
D002257:Carbonic Anhydrase Inhibitors; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases; D000086:Acetazolamide