title: Reproducibility of Bordetella pertussis genomic DNA fragments generated by XbaI restriction and resolved by pulsed-field gel electrophoresis.
abstract: The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified.
authors.affiliation: Meningitis and Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
authors.forename: Terri H
authors.initials: TH
authors.lastname: Hardwick
authors.forename: Brian
authors.initials: B
authors.lastname: Plikaytis
authors.forename: Pamela K
authors.initials: PK
authors.lastname: Cassiday
authors.forename: Gary
authors.initials: G
authors.lastname: Cage
authors.forename: Mark S
authors.initials: MS
authors.lastname: Peppler
authors.forename: Deborah
authors.initials: D
authors.lastname: Shea
authors.forename: David
authors.initials: D
authors.lastname: Boxrud
authors.forename: Gary N
authors.initials: GN
authors.lastname: Sanden
mesh_terms: D001886:Bordetella pertussis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D015203:Reproducibility of Results
chemical_list: D004269:DNA, Bacterial; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific