Detection of a quantitative trait locus for intramuscular fat accumulation using the OLETF rat.
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an animal model for obese type 2 diabetes. We showed that the OLETF rat exhibits higher levels of intramuscular fat content in Musculus longissimus as compared to the Fischer-344 (F344) rat. Our investigation was designed to identify quantitative trait loci (QTLs) contributing to the increased levels of intramuscular fat content by performing a whole-genome search using 108 F2 intercross obtained by mating the OLETF and the F344 rats. We identified one QTL responsible for intramuscular fat accumulation on rat chromosome 1 with a maximum lod score of 3.4, which accounts for 5% of the total variance. As expected, the OLETF allele corresponds to the increased levels of intramuscular fat content.
Tanomura
Hideki
H
Laboratory of Animal Breeding and Genetics, Graduate School of Agriculture, Kyoto University, Japan.
Miyake
Takeshi
T
Taniguchi
Yukio
Y
Manabe
Noboru
N
Kose
Hiroyuki
H
Matsumoto
Kozo
K
Yamada
Takahisa
T
Sasaki
Yoshiyuki
Y
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D019655:Quantitative Trait, Heritable; D051381:Rats; D011916:Rats, Inbred F344 / Q000235:genetics* / Q000378:metabolism; D020334:Rats, Inbred OLETF / Q000235:genetics* / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms