title: Detection of the gp43 gene and (1-3)-beta-D-glucan of Paracoccidioides brasiliensis in the blood of experimentally infected mice.
abstract: Paracoccidioidomycosis (PCM) is a deep mycosis caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. Diagnosis of PCM is sometimes difficult outside the endemic countries, thus a rapid and conclusive method for diagnosis of PCM has been anticipated. We compared the sensitivities of a nested PCR method for detecting the gp43 gene and a commercial kit for detecting (1-3)-beta-D-glucan in the blood of experimentally infected mice. Blood samples were collected from mice at 0 (soon after inoculation), 6, 12, 24, 48, and 72 hours and 5, 7, 10, 14, 17, 21, 24, 28 and 56 days after the intravenous inoculation of 10(6) yeast cells of P. brasiliensis, and were separated into clots and plasma. The (1-3)-beta-D-glucan detection kit in the plasma showed positive reactions in some samples within 7 days and 28 and 56 days after infections. In contrast, the PCR method was more sensitive than the (1-3)-beta-D-glucan detection kit throughout the observation period. The clot samples yielded more sensitive PCR-results than did the plasma samples. Although 24 hours is required for the PCR detection, it was confirmed to provide an accurate diagnosis of PCM.
authors.affiliation: Universidade Estadual de Londrina, CCB-PAT-Campus Universitario, CEP: 86051-970, Londrina, Parana, Brazil.
authors.forename: Eiko
authors.initials: E
authors.lastname: Nakagawa Itano
authors.forename: Jun
authors.initials: J
authors.lastname: Uno
authors.forename: Ayako
authors.initials: A
authors.lastname: Sano
authors.forename: Kyoko
authors.initials: K
authors.lastname: Yarita
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kamei
authors.forename: Makoto
authors.initials: M
authors.lastname: Miyaji
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nishimura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Mikami
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000946:Antigens, Fungal; D015415:Biomarkers / Q000097:blood; D002648:Child; D011756:Diphosphates; D004195:Disease Models, Animal; D004338:Drug Combinations; D005260:Female; D005656:Fungal Proteins; D005800:Genes, Fungal; D005936:Glucans / Q000097:blood*; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008875:Middle Aged; D009566:Nitrates; D010228:Paracoccidioides / Q000235:genetics*; D010229:Paracoccidioidomycosis / Q000175:diagnosis*; D011095:Polyethylenes; D016133:Polymerase Chain Reaction / Q000379:methods; D012969:Sodium Fluoride; D047071:beta-Glucans
chemical_list: C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D015415:Biomarkers; D011756:Diphosphates; D004338:Drug Combinations; D005656:Fungal Proteins; D005936:Glucans; D006023:Glycoproteins; D009566:Nitrates; D011095:Polyethylenes; C097426:Sensitive Tartar Control Dentifrice; D047071:beta-Glucans; D012969:Sodium Fluoride; C033363:beta-1,3-glucan