TRPC4 can be activated by G-protein-coupled receptors and provides sufficient Ca(2+) to trigger exocytosis in neuroendocrine cells.
Transient receptor potential (TRP) channels form a large family of plasma membrane cation channels. Mammalian members of the "short" TRP family (TRP channel (TRPC) 1-7 are Ca(2+)-permeant, non-selective cation channels that are widely expressed in various cell types, including neurons. TRPC activity is linked through unknown mechanisms to G-protein-coupled receptors or receptor tyrosine kinases that activate phospholipase C. To investigate the properties and function of TRPC4 in neuronally derived cells, we transiently expressed mouse TRPC4 and histamine H(1) receptor in mouse adrenal chromaffin cells and PC12 cells. Histamine, but not thapsigargin, stimulated Mn(2+) influx in transfected cells. In the whole-cell patch clamp mode, histamine triggered a transient current in TRPC4-expressing cells. No current was evoked by perfusion with inositol 1,4,5-trisphosphate. When exocytosis was monitored with the capacitance detection technique, the magnitude of the membrane capacitance increase (Delta C(m)) on application of histamine in H(1) receptor/TRPC4-expressing chromaffin cells was comparable with that triggered by a train of depolarizing pulses. Our results indicate that TRPC4 channels behave as receptor, but not store-operated, channels in neuronally derived cells. TRPC4 channels can provide sufficient Ca(2+) influx to trigger a robust secretory response in voltage-clamped neurosecretory cells. Similar mechanisms may modulate exocytosis in other neuronal systems.
Obukhov
Alexander G
AG
Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA.
Nowycky
Martha C
MC
D000302:Adrenal Cortex / Q000502:physiology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology / Q000502:physiology*; D005089:Exocytosis / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D008345:Manganese / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D009490:Neurosecretory Systems / Q000502:physiology*; D016716:PC12 Cells; D020033:Protein Isoforms / Q000502:physiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology; D050052:TRPC Cation Channels; D019284:Thapsigargin / Q000494:pharmacology; D014162:Transfection
D015220:Calcium Channels; D007473:Ion Channels; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D050052:TRPC Cation Channels; D008345:Manganese; D019284:Thapsigargin; D006632:Histamine; D019204:GTP-Binding Proteins; D002118:Calcium