Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins.
We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.
Schmidt
Frank
F
Max Planck Institute for Molecular Genetics, Berlin, Germany.
Lueking
Angelika
A
Nordhoff
Eckhard
E
Gobom
Johan
J
Klose
Joachim
J
Seitz
Harald
H
Egelhofer
Volker
V
Eickhoff
Holger
H
Lehrach
Hans
H
Cahill
Dolores J
DJ
D001921:Brain / Q000196:embryology; D003001:Cloning, Molecular; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005333:Fetus; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D019151:Peptide Library; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D010446:Peptide Fragments; D019151:Peptide Library; D011506:Proteins; D020543:Proteome; D011994:Recombinant Proteins