Immunization of T-cell deficient mice against polyomavirus infection using viral pseudocapsids or temperature sensitive mutants.
A murine experimental model system aimed at developing potential vaccines to papovavirus infection in immunosuppressed individuals was explored. A VP1-pseudocapsid based on the major capsid protein of the murine polyomavirus A2 strain and a mutant, M17-pseudocapsid as well as four temperature sensitive (ts)-mutants were used as immunogens. T-cells deficient CD4-/-8-/- mice were immunized four times with each immunogen and then together with non-immunized control mice challenged with polyomavirus. In contrast to all control mice, only half of the immunized mice exhibited presence of polyoma DNA when assayed by PCR. The results indicate that pseudocapsids and ts-mutant immunization may potentially protect mice with an impaired T-cell function from polyomavirus infection.
Heidari
Shirin
S
Division of Experimental Oncology, Cancer Centre Karolinska, Karolinska Hospital, SE-17176 Stockholm, Sweden. shirin.heidari@mtc.ki.se
Vlastos
Andrea
A
Ramqvist
Torbjörn
T
Clark
Barny
B
Griffin
Beverly E
BE
Garcia
Marie Isabelle
MI
Perez
Marie
M
Amati
Paolo
P
Dalianis
Tina
T
D000818:Animals; D015704:CD4 Antigens / Q000235:genetics; D016827:CD8 Antigens / Q000235:genetics; D002213:Capsid / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D007114:Immunization; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics* / Q000276:immunology*; D027601:Polyomavirus Infections / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D013601:T-Lymphocytes / Q000276:immunology*; D013696:Temperature; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D015704:CD4 Antigens; D016827:CD8 Antigens; D036022:Capsid Proteins; C062481:VP1 protein, polyomavirus; D014765:Viral Vaccines