Lipopolysaccharide-specific but not anti-flagellar immunoglobulin A monoclonal antibodies prevent Salmonella enterica serotype enteritidis invasion and replication within HEp-2 cell monolayers.
The protective potential of immunoglobulin A (IgA) monoclonal antibodies (MAbs) directed against O and H antigens of Salmonella enterica serotype Enteritidis to prevent bacterial adhesion to and invasion of HEp-2 cells was evaluated. Although anti-flagellar IgA MAbs showed strong agglutinating capacities, they did not protect cell monolayers. In contrast, IgA MAbs specific for the O:9 epitope of Salmonella lipopolysaccharide antigen alone prevented S. enterica serotype Enteritidis entry and replication within HEp-2 cells, and the protection was not mediated by direct binding of antibodies to bacterial adhesins or by agglutination of microorganisms.
Iankov
Ianko D
ID
Department of Microbiology, Medical University of Sofia, 1431 Sofia, Bulgaria.
Petrov
Dragomir P
DP
Mladenov
Ivan V
IV
Haralambieva
Iana H
IH
Mitov
Ivan G
IG
D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000918:Antibody Specificity; D001422:Bacterial Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005407:Flagella / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000276:immunology*; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D008070:Lipopolysaccharides