title: The role of hypo-osmotic swelling test in recurrent abortions.
abstract: The role of male factors in abortions has not been studied extensively. We undertook this study to determine if there was any relationship between hypo-osmotic swelling test score and recurrent abortions. This was a cross-sectional case control study conducted at the semen analysis laboratory at a tertiary level referral centre. The male partners of 25 patients who had three or more first trimester abortions of unknown etiology were chosen as cases. Controls were 26 unmarried volunteers with unknown fertility potential to represent the entire population. The conventional seminal parameters were studied according to WHO guidelines. The HOS test score were obtained by the method advocated by Jayendran et al. There were no statistically significant differences in mean sperm count, percentage motility and morphology among the cases and controls. We found a low hypo-osmotic swelling test score among the cases [55.7 +/- 1.197 (SEM)] than the controls [69.3 +/- 1.143 (SEM)] which was statistically significant [P < 0.001]. The normal spermatozoal membrane is the prerequisite for the specialized cell-to-cell communications and cell-to-cell binding. In spite of apparently normal semen analysis, subtle membrane defects in the spermatozoa, which could be the cause of defective membrane functions in the embryo effecting miscarriages, can be elucidated by the hypo-osmotic swelling test.
authors.affiliation: Department of Physiology, Govt. Medical College, Nagpur-440 010.
authors.forename: S S
authors.initials: SS
authors.lastname: Patankar
authors.forename: A M
authors.initials: AM
authors.lastname: Deshkar
authors.forename: M V
authors.initials: MV
authors.lastname: Sawane
authors.forename: N V
authors.initials: NV
authors.lastname: Mishra
authors.forename: A H
authors.initials: AH
authors.lastname: Kale
authors.forename: G B
authors.initials: GB
authors.lastname: Gosavi
mesh_terms: D000026:Abortion, Habitual / Q000473:pathology*; D000328:Adult; D048429:Cell Size; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009845:Oligospermia / Q000473:pathology; D009997:Osmotic Pressure; D012661:Semen / Q000166:cytology / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*