Expression and self-assembly of norwalk virus capsid protein from venezuelan equine encephalitis virus replicons.
The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis virus replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like particles that were primarily cell associated and were indistinguishable from NV particles produced from baculoviruses. Mutations located in the N-terminal and P1 domains of the NV capsid protein ablated capsid self-assembly in mammalian cells.
Baric
Ralph S
RS
Department of Epidemiology, Program in Infectious Diseases, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7400, USA. rbaric@sph.unc.edu
Yount
Boyd
B
Lindesmith
Lisa
L
Harrington
Patrick R
PR
Greene
Shermalyn R
SR
Tseng
Fan-Chen
FC
Davis
Nancy
N
Johnston
Robert E
RE
Klapper
David G
DG
Moe
Christine L
CL
D000818:Animals; D018938:Caco-2 Cells; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D009663:Norwalk virus / Q000378:metabolism*; D012093:Replicon; D019065:Virus Assembly