[Pressure influence on mRNA expression and protein synthesis of inducible nitric oxide synthetase in bovine trabecular meshwork cell].
OBJECTIVE
To investigate the inducible nitric oxide synthetase (iNOS) mRNA expression and protein synthesis in bovine trabecular meshwork cell and discuss the possible effects of nitric oxide (NO) in the development of glaucoma.


METHODS
20 mm Hg, 40 mm Hg, 60 mm Hg and 80 mm Hg pressure were respectively added on cultured trabecular cells of new born bovine. No pressure group was set as the control. The changes of iNOS mRNA and protein in trabecular meshwork cells under different pressures were demonstrated qualitatively and quantitatively by in situ hybridization and nicotinamide-adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical assay.


RESULTS
The expression of iNOS mRNA and its protein synthesis became higher and higher as the pressure was increased. There was weak iNOS mRNA expression in the control and 20 mm Hg groups, and no significant difference between them. 40 mm Hg and 60 mm Hg groups had a statistical difference from the control group. And 80 mm Hg group had a very significant difference from the control group. There was no difference between 40 mm Hg and 60 mm Hg groups, but they had significant difference from 80 mm Hg group. NADPH-d histochemical assay showed almost the same results except that there was significant difference between 40 mm Hg and 60 mm Hg groups.


CONCLUSIONS
Pressure can evoke the expression of iNOS mRNA, and the nitric oxide thus produced can be one of the causes of trabecular meshwork destruction, that may induce or aggravate glaucoma.
Xue
W
W
Department of Ophthalmology, Tongji Hospital, Tongji Medical University, Wuhan 430030, China. xuewei@public.wuhan.cngb.com
Du
S
S
Li
Y
Y
Huang
K
K
D000818:Animals; D001274:Atmospheric Pressure; D002417:Cattle; D002478:Cells, Cultured; D005901:Glaucoma / Q000201:enzymology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D014129:Trabecular Meshwork / Q000201:enzymology* / Q000378:metabolism
D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II