O-Specific chain structure from the lipopolysaccharide fraction of Pseudomonas reactans: a pathogen of the cultivated mushrooms.
An O-specific polysaccharide containing 2-acetamidino-2-deoxy-beta-D-glucopyranose (Glcp2Am), 2,4-diacetamido-2,4,6-trideoxy-beta-D-glucopyranose (QuipNAc4NAc, bacillosamine) and 2,4-di-(N-acetyl-L-alanylamino)-2,4,6-trideoxy-beta-D-glucopyranose (QuipNAlaAc4NAlaAc) was isolated from the phenol-soluble lipopolysaccharide fraction of the mushroom-associated bacterium Pseudomonas reactans. The structure, determined by means of chemical analysis and 1D and 2D NMR spectroscopy, showed a linear trisaccharide-repeating unit, as shown below:-->3)-beta-D-QuipNAlaAc4NAlaAc-(1-->3)-alpha-D-Glcp2Am-(1-->3)-alpha-D-QuipNAc4NAc(1-->To our knowledge, this is the first complete O-chain structure reported for the lipopolysaccharide of a mushroom-associated bacterium.
Molinaro
Antonio
A
Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, via Cintia 4, I-80126, Naples, Italy. molinaro@unina.it
Evidente
Antonio
A
Sante Iacobellis
Nicola
N
Lanzetta
Rosa
R
Lo Cantore
Pietro
P
Mancino
Anna
A
Parrilli
Michelangelo
M
D000363:Agaricales; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011549:Pseudomonas / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry
D008070:Lipopolysaccharides; D019081:O Antigens; D014312:Trisaccharides