[Effect of fodder components on the product of obese gene expression--leptin in rats].
OBJECTIVE
To study the effects of different fodder components and levels of caloric uptake on the product of obese gene expression -- leptin and in vivo secretion of insulin and growth hormone, and to study the relationship between them in rats.


METHODS
Plasma leptin was determined by Western blot, and serum insulin and growth hormone were determined by radioimmunoassay (RIA).


RESULTS
There was no significant effects of fodder components, including high protein, fat and carbohydrate, on the levels of leptin, insulin and growth hormone in rats, with the same caloric uptake in them. However, with the higher caloric uptake, level of obese gene expression increased, showing increased levels of plasma leptin and serum insulin and decreased level of serum growth hormone.


CONCLUSIONS
There could existed an interaction between expression of obese gene and secretions of leptin, insulin and growth hormone, which could be modified by the same metabolic signal.
Sun
C
C
Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin, 150001.
Zhang
H
H
Zhu
Y
Y
Chen
B
B
He
H
H
Hao
J
J
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002149:Energy Intake; D005247:Feeding Behavior; D005260:Female; D005786:Gene Expression Regulation; D013006:Growth Hormone / Q000097:blood*; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000097:blood* / Q000235:genetics; D008297:Male; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D020738:Leptin; D013006:Growth Hormone