Recombinase-directed plant transformation for the post-genomic era.
Plant genomics promises to accelerate genetic discoveries for plant improvements. Machine-driven technologies are ushering in gene structural and expressional data at an unprecedented rate. Potential bottlenecks in this crop improvement process are steps involving plant transformation. With few exceptions, genetic transformation is an obligatory final step by which useful traits are engineered into plants. In addition, transgenesis is most often needed to confirm gene function, after deductions made through comparative genomics, expression profiles, and mutation analysis. This article reviews the use of recombinase systems to deliver DNA more efficiently into the plant genome.
Ow
David W
DW
Plant Gene Expression Center, Agricultural Research Service, United States Department of Agriculture, Albany, CA 94710, USA. ow@pgec.ars.usda.gov
D001483:Base Sequence; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D018745:Genome, Plant; D019426:Integrases; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D045522:Recombinases; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
D018744:DNA, Plant; D045522:Recombinases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1