title: Inhibition of heat shock-induced apoptosis by peppermint oil in astrocytes.
abstract: Exposure to environmental stresses and toxins is linked to the pathogenesis of neuropsychiatric disorders. Astrocytes, the most abundant glial-cell type in the brain, are considered to have physiological and pathological roles in neuronal activities. We have investigated whether peppermint oil inhibits heat shock-induced apoptosis of astrocytes. We found that peppermint oil inhibits the heat shock-induced apoptosis in both human astrocyte CCF-STTG1 cells and rat astrocytes. Pretreatment of the cells with peppermint oil inhibited the heat shock-induced DNA fragmentation and condensation of nuclear chromatin. Peppermint oil also inhibited the caspase-3 activation and poly-ADP-ribose polymerase fragmentation in CCF-STTG1 cells. These results suggest that peppermint oil may modulate the apoptosis of astrocytes via the activation of the caspase-3.
authors.affiliation: College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: H N
authors.initials: HN
authors.lastname: Koo
authors.forename: H J
authors.initials: HJ
authors.lastname: Jeong
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: S T
authors.initials: ST
authors.lastname: Park
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: K K
authors.initials: KK
authors.lastname: Seong
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Lyu
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D053148:Caspase 3; D020169:Caspases / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D053938:DNA Fragmentation / Q000187:drug effects; D004586:Electrophoresis; D004789:Enzyme Activation / Q000187:drug effects; D005434:Flow Cytometry; D006358:Hot Temperature / Q000009:adverse effects*; D006801:Humans; D036142:Mentha piperita; D010938:Plant Oils / Q000494:pharmacology*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D051381:Rats
chemical_list: D010938:Plant Oils; D011506:Proteins; C015424:peppermint oil; C499320:PARP1 protein, human; C499322:Parp1 protein, rat; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases