[Liver transplantation with no-heatbeating donor supplied gaseous oxygen during cold preservation in pigs].
OBJECTIVE
To investigate the availability of liver transplantation with non-heart beating (60 min) donors supplied gaseous oxygen by hepatic vein in cold preservation period in pigs.


METHODS
Thirty-six pigs were randomly divided into three recipient groups and three donor groups (group A-15 min of warm ischaemia, group B-60 min of warm ischaemia and group C-60 min of warm ischaemia with gaseous oxygen by hepatic vein during cold preservation period). OTL was performed after four hours cold preservation. Postoperative 5 days survival rate of the liver metabolic, function and inflammatory reaction were observed.


RESULTS
All animals in group A and C survived after reperfusion for 5 days, but animals in group B died within 3 hours postreperfusion. The damage of liver construction and function were found in group A and group B. There was no significant difference of the changes between group A and C.


CONCLUSIONS
Gaseous oxygen persufflation during cold preservation has important protective and resuscitative effect for liver from long-time no-heart beating donors. It was possible to become a promising method for liver transplantation with no-hearbeating donors.
Fu
Zhongxue
Z
*Department of General Surgery of the First Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China.
Saad
Stefan
S
Nagelschmidt
Manfred
M
Han
Wenmiao
W
D000818:Animals; D015925:Cryopreservation; D006323:Heart Arrest / Q000503:physiopathology; D006503:Hepatic Veins; D008099:Liver / Q000503:physiopathology*; D008111:Liver Function Tests; D016031:Liver Transplantation; D010100:Oxygen / Q000627:therapeutic use*; D015424:Reperfusion; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013552:Swine; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods
D013482:Superoxide Dismutase; D010100:Oxygen