Brain targeting and glomerulus formation of two olfactory neuron populations expressing related receptor types.
Olfactory sensory neurons expressing different members of the mOR37 odourant receptor subfamily send their axons to distinct glomeruli located in the immediate vicinity in the olfactory bulb [Strotmann, J., Conzelmann, S., Beck, A., Feinstein, P., Breer, H. & Mombaerts, P. (2000) J. Neurosci., 20, 6927-6938]. In this study, the potential of transgenic mouse lines was used to explore the onset of receptor expression, the outgrowth of axons as well as the glomerulus formation for two neuron populations expressing different mOR37 subtypes. The data indicate a synchronous time course of these features for both neuron populations. From E15 until the day of birth, the axons of the two mOR37 populations terminate in a common, small area of the presumptive olfactory bulb. During a short postnatal phase, the two axon populations segregate into distinct, protoglomerular structures; some aberrant fibers can still be observed during this period.
Conzelmann
S
S
Institute of Physiology, University Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany.
Malun
D
D
Breer
H
H
Strotmann
J
J
D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017930:Genes, Reporter / Q000502:physiology; D049452:Green Fluorescent Proteins; D020439:Growth Cones / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D009832:Olfactory Nerve / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000196:embryology* / Q000254:growth & development; D012333:RNA, Messenger / Q000378:metabolism; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism* / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D007202:Indicators and Reagents; D008164:Luminescent Proteins; C452230:Olfr37 protein, mouse; D012333:RNA, Messenger; D018035:Receptors, Odorant; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase