Cloning of the PpMSH-2 cDNA of Physcomitrella patens, a moss in which gene targeting by homologous recombination occurs at high frequency.
In the moss Physcomitrella patens integrative transformants from homologous recombination are obtained at an efficiency comparable to that found for yeast. This property, unique in the plant kingdom, allows the knockout of specific genes. It also makes the moss a convenient model to study the regulation of homologous recombination in plants. We used degenerate oligonucleotides designed from AtMSH2 from Arabidopsis thaliana and other known MutS homologues to isolate the P. patens MSH2 (PpMSH2) cDNA. The deduced sequence of the PpMSH2 protein is respectively 60.8% and 59.6% identical to the maize and A. thaliana MSH2. Phylogenic studies show that PpMSH2 is closely related to the group of plant MSH2 proteins. Southern analysis reveals that the gene exists as a single copy in the P. patens genome.
Brun
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Laboratoire de Biologie Cellulaire, INRA, route de St.-Cyr, 78026 Versailles, France.
Gonneau
M
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Doutriaux
M P
MP
Laloue
M
M
Nogué
F
F
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D020137:Base Pair Mismatch / Q000235:genetics; D015139:Blotting, Southern; D019068:Bryopsida / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D004260:DNA Repair / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018390:Gene Targeting / Q000379:methods; D018745:Genome, Plant; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051718:MutS Homolog 2 Protein; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017385:Sequence Homology
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; C108984:MSH2 protein, Arabidopsis; D051718:MutS Homolog 2 Protein