[Characterization of Vibrio cholerae eltor isolates according to their epidemic potential using new diagnostic cholera bacteriophages eltor ctx+ and ctx- and by the polymerase chain reaction].
The epidemic potential of 113 V. cholerae eltor strains of different origin was determined with new diagnostic cholera bacteriophages eltor ctx+ and ctx-, as well as the test for hemolytic activity. Of these strains 50 were epidemically safe and 51 were epidemically dangerous, while the epidemic potential of 12 other strains could not be detected. Determination of genes ctxA, tcpA and toxR in the strains under study by means of the polymerase chain reaction (PCR) revealed that epidemically dangerous strains carried the whole set of the above genes in 92.2% of cases. 98.0% of epidemically safe cultures were lacking either gene ctxA, or genes ctxA and tcpA, or genes ctxA, tcpA and toxR, which confirmed their incapacity to cause cholera. The results of the differentiation of the cultures with new diagnostic cholera phages coincided with the results of PCR in 90% of cases. The most complete and reliable evaluation of the epidemic potential of individual vibrio isolates may be obtained using the two compared methods. The amplification test system gives more information when isolates with unclear epidemic potential are analyzed.
Smirnova
N I
NI
Research Institute for Plague Control Microbe, Saratov, Russia.
Cheldyshova
N B
NB
Kostromitina
E A
EA
Kulichenko
A N
AN
Kutyrev
V V
VV
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics; D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics; D002771:Cholera / Q000175:diagnosis / Q000635:transmission; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D005814:Genes, Viral; D005819:Genetic Markers; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics; D014734:Vibrio cholerae / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D006460:Hemolysin Proteins; C085807:TcpA protein, Vibrio cholerae; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; D036781:Fimbriae Proteins