Biosynthesis of zeaxanthin via mevalonate in Paracoccus species strain PTA-3335. A product-based retrobiosynthetic study.
Cultures of the zeaxanthin-producing bacterium Paracoccus species strain PTA-3335 (formerly Flavobacterium) were grown with supplements of (13)C-labeled glucose. Zeaxanthin was isolated and analyzed by (13)C NMR spectroscopy. The data showed that the isoprenoid precursors of zeaxanthin were biosynthesized via the mevalonate pathway. The microorganism was found to utilize glucose mainly via the Entner-Doudoroff pathway.
Eisenreich
Wolfgang
W
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Federal Republic of Germany. wolfgang.eisenreich@ch.tum.de
Bacher
Adelbert
A
Berry
Alan
A
Bretzel
Werner
W
Hümbelin
Markus
M
Lopez-Ulibarri
Rual
R
Mayer
Anne F
AF
Yeliseev
Alexei
A
D009682:Magnetic Resonance Spectroscopy; D008798:Mevalonic Acid / Q000378:metabolism*; D015394:Molecular Structure; D010230:Paracoccus / Q000378:metabolism*; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000737:chemistry
D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D008798:Mevalonic Acid