title: [Expression of membrane-type I matrix metalloproteinase and its correlation with gelatinase A activation in cancer cell lines].
abstract: OBJECTIVE
  To study the expression of membrane-type I matrix metalloproteinase (MT1-MMP) and its correlation with gelatinase A (MMP2) activation.
  
  
  METHODS
  Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1-MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines.
  
  
  RESULTS
  All the cancer cell lines expressed MT1-MMP mRNA, and the MT1-MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines. Activated MMP2 proteins were only detected in the melanoma cell lines, whereas PG, a lung cancer cell line, which secreted pro-MMP2 and expressed low level of MT1-MMP, could not produce activated MMP2.
  
  
  CONCLUSIONS
  The level of MT1-MMP expression was highly associated with MMP2 activation.
authors.affiliation: Department of Pathology, Beijing Medical University, Beijing 100083.
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
authors.forename: B
authors.initials: B
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: You
authors.forename: H
authors.initials: H
authors.lastname: Zhong
authors.forename: W
authors.initials: W
authors.lastname: Fang
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000235:genetics* / Q000378:metabolism; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2