Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss).
As part of an effort to validate the use of heat shock proteins (HSPs) as biomarkers of exposure to and effects of contaminants, we evaluated the effect of two handling regimens on the induction of HSP 60 and 70 in rainbow trout (Oncorhynchus mykiss). Fish were acclimated to laboratory conditions for several weeks before the beginning of the experiment. Fish were then captured by net, placed in a cooler for 1 h while being transported in a truck, returned to their original tanks, then sacrificed 6 to 8 h later. Tricaine methane sulfonate (MS-222) was used during different phases of handling to reduce handling stress. Heat-stressed fish were included in the experiment as a positive control. Muscle, liver, gills, and heart were analyzed for HSP 60 and 70 by immunoblotting. We found no effect of any handling regimen on the induction of HSPs. These findings suggest that the capture and transport of fish for environmental monitoring purposes should not interfere with the use of stress proteins as biomarkers.
Washburn
Barbara Shayne
BS
Department of Biological Sciences, University of Texas, El Paso 79968, USA. bwashburn@oehha.ca.gov
Moreland
Jason J
JJ
Slaughter
Ann M
AM
Werner
Inge
I
Hinton
David E
DE
Sanders
Brenda M
BM
D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D018834:Chaperonin 60 / Q000096:biosynthesis*; D005880:Gills / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D015151:Immunoblotting; D008099:Liver / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000502:physiology*; D015203:Reproducibility of Results; D013048:Specimen Handling; D014018:Tissue Distribution
D015415:Biomarkers; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins