title: 2001 Harry M. Vars Research Award. Enteral nutrients alter enterocyte function within an in vitro model similar to an acute in vivo rat model during hypoxia.
abstract: BACKGROUND
  Early enteral nutrition in patients following traumatic injury is an important intervention. However, after shock-resuscitation, intestinal hypoperfusion persists despite adequate systemic resuscitation. Our previous in vivo rat studies indicate that hypoperfusion impairs mucosal function in the small intestine. Therefore, the current study sought to improve previous in vitro models by the following means: (1) We used Caco-2 monolayers stably transfected with the brush-border sodium-glucose co-transporter (SGLT-1); and (2) we created an environment that mimicked the physiologic enterocyte environment. We hypothesized that hypoxic alterations of epithelial function in an in vitro model are comparable to those of an in vivo rat model.
  
  
  METHODS
  After 21 days, monolayers were randomized to receive 24 hours of incubation in a normoxic or hypoxic environment. Cells were further randomized to receive 1 of 4 nutrient treatments: mannitol (an osmotic control), glucose (uses SGLT-1 and is metabolized), 3-O-methylglucose (3-O-mg; uses SGLT-1 and is not metabolized), or fructose (does not use SGLT-1 but can be metabolized).
  
  
  RESULTS
  Transepithelial resistance (p = .007) and short-circuit current (p = .05) were lower in hypoxic groups. When compared with normoxic groups, hypoxic groups had significantly impaired glucose (p < .001) but not glutamine transport, irrespective of nutrient treatment. Additionally, adenosine triphosphate/adenosine diphosphate ratio was reduced (p = .01) and lactate concentration was increased (p < .001) during hypoxia.
  
  
  CONCLUSIONS
  In summary, results from this in vitro study using Caco-2BBe cells stably transfected with SGLT-1 correspond to results obtained in the in vivo rat model. Therefore, this is an appropriate in vitro model in which to study cellular alterations caused by the hypoxic small intestine, with the goal of ensuring safe early enteral nutrition following traumatic injury.
authors.affiliation: Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, 61801, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Kles
authors.forename: J R
authors.initials: JR
authors.lastname: Turner
authors.forename: K A
authors.initials: KA
authors.lastname: Tappenden
mesh_terms: D019325:3-O-Methylglucose / Q000008:administration & dosage; D000244:Adenosine Diphosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D018938:Caco-2 Cells; D015687:Cell Hypoxia; D004553:Electric Conductivity; D017097:Electric Impedance; D004750:Enteral Nutrition; D020895:Enterocytes / Q000737:chemistry / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D005632:Fructose / Q000008:administration & dosage; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000032:analysis; D008353:Mannitol / Q000008:administration & dosage; D008562:Membrane Glycoproteins / Q000235:genetics / Q000502:physiology; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000502:physiology; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D051269:Sodium-Glucose Transporter 1; D014162:Transfection
chemical_list: D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; C495674:SLC5A1 protein, human; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D005973:Glutamine; D019325:3-O-Methylglucose; D005632:Fructose; D019344:Lactic Acid; D008353:Mannitol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D005947:Glucose