Simultaneous high-performance liquid chromatographic determination of paracetamol, phenylephrine HCl, and chlorpheniramine maleate in pharmaceutical dosage forms.
A rapid, precise, and specific high-performance liquid chromatographic method is described for the simultaneous determination of paracetamol, phenylephrine HCI, and chlorpheniramine maleate in combined pharmaceutical dosage forms. The method involves the use of a microBondapak CN RP analytical column (125 A, 10 microm, 3.9 x 150 mm) at 22 degrees C as the stationary phase with the mixture of acetonitrile and phosphate buffer (pH 6.22, 78:22) as the mobile phase. Derivatization of the drugs is not required. The method is applied to commercial pediatric cough-cold syrups, tablets, and capsules marketed in Turkey. The relative standard deviation for 10 replicate measurements of each drug in the medicaments is always less than 2%.
Senyuva
Hamide
H
Instrumental Analysis Centre, Scientific and Technical Research Council of Turkey, Ankara. hsenyuva@tubitak.gov.tr
Ozden
Tuncel
T
D000082:Acetaminophen / Q000032:analysis*; D002138:Calibration; D002744:Chlorpheniramine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004304:Dosage Forms; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010656:Phenylephrine / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D004304:Dosage Forms; D004364:Pharmaceutical Preparations; D010656:Phenylephrine; D000082:Acetaminophen; D002744:Chlorpheniramine