[Study on the protective effect of grape procyanidins on DNA damage induced by irradiation].
OBJECTIVE
To study the protective effect of grape procyanidins (GPC) on the irradiated DNA damage.


METHODS
In vivo study: After orally administration of GPC, mice in different groups were exposed to whole-body (60)Co gamma-rays at a dose of 2.0 Gy and the fluorospectrometry method was used to rapidly detect the DNA strand breaks of mouse spleen cells. In vitro study: Human venous blood with different concentrations of GPC was exposed to (60)Co gamma-rays at a dose of 1.0 Gy, then WBC DNA content was measured after 1, 30 and 60 min exposure, respectively.


RESULTS
In the three treated groups (500, 250 and 50 mg/kg of procyanidins), DNA content of mouse spleen cells was 18.76%, 15.74% and 5.06% more than that of the positive controls respectively. DNA content of human WBC was higher than the positive controls by 17.6%, 11.1% and 3.9% increase respectively one minute after irradiation and 21.3%, 13.8% and 5.3% increase respectively 60 min after irradiation. These differences were all statistically significant.


CONCLUSIONS
GPC has a protective effect on DNA damage caused by irradiation, and is helpful to DNA repair.
Li
S
S
Department of Nutrition, Medical College of Qingdao University, Qingdao 266021, China.
Zhong
J
J
Sun
F
F
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D003037:Cobalt Radioisotopes; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D044945:Proanthocyanidins; D011833:Radiation Injuries, Experimental / Q000473:pathology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D027843:Vitis / Q000737:chemistry; D014916:Whole-Body Irradiation
D000975:Antioxidants; D044946:Biflavonoids; D003037:Cobalt Radioisotopes; D044945:Proanthocyanidins; D011837:Radiation-Protective Agents; C017674:procyanidin; D002392:Catechin