[Modified oligonucleotides containing 1-beta-D-galactopyranosylthymine: synthesis and substrate properties].
A convenient method of regioselective introduction of 1-beta-D-galactopyranosylthymine into oligonucleotides was developed and the substrate properties of the modified oligonucleotides were investigated in the enzymic reaction of formation and hydrolysis of internucleotide bonds. The English version of the paper.
Ermolinskiĭ
B S
BS
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow, 117984 Russia.
Fomicheva
M V
MV
Efimtseva
E V
EV
Mikhaĭlov
S N
SN
Esipov
D S
DS
Boldyreva
E F
EF
Korobko
V G
VG
van Aerschot
A
A
Herdewijn
P
P
D001671:Biochemistry / Q000379:methods*; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000378:metabolism; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013941:Thymine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism*
C460040:1-galactopyranosylthymine; D009841:Oligonucleotides; D015246:Deoxyribonuclease EcoRI; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; D013941:Thymine; D005690:Galactose