[The effects of cyclin E on the growth and other cell cycle related genes of breast carcinoma cells MCF-7].
OBJECTIVE
To investigate the effects of cyclin E in high and low expression on the cell growth and other cell cycle associated genes of MCF-7 cells.


METHODS
Eukaryotic expression vehicles of sense and antisense cyclin E were constructed and transferred into the MCF-7 cell line using lipofectAMINE. The integration and expression of cyclin E were conformed by Southern and Western blot. The cells growth was observed and the changes in cell cycle were analyzed by flow cytometry. The expression of other cell cycle associated genes was assayed using Western blot.


RESULTS
A high expression of cyclin E enabled to promote the cell growth and DNA synthesis and accelerated the proceeding of G(1) phase to S phase, It also promoted the phosphorylatin of pRB and up-regulate the expression of p27 while a low expression of cyclin E showed an opposite effect.


CONCLUSIONS
Different expression of cyclin E enables to affect growth of MCF-7 cell by the changes of cell cycle related genes.
Huang
M
M
Department of Pathology, Beijing Medical University, Beijing 100083, China.
Yang
S
S
Liao
S
S
Zhang
B
B
You
J
J
D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D002455:Cell Division; D045744:Cell Line, Tumor; D019927:Cyclin E / Q000096:biosynthesis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D010766:Phosphorylation; D016372:RNA, Antisense / Q000096:biosynthesis / Q000494:pharmacology; D016160:Retinoblastoma Protein / Q000378:metabolism; D014162:Transfection
D018797:Cell Cycle Proteins; D019927:Cyclin E; D004273:DNA, Neoplasm; D016372:RNA, Antisense; D016160:Retinoblastoma Protein