Cloning and characterization of the human BAZF gene, a homologue of the BCL6 oncogene.
The BAZF gene has recently been identified as a novel homologue of the BCL6 oncogene. Here we cloned the human BAZF gene using murine BAZF as a probe. The predicted amino acid sequence was 91% identical to that of murine BAZF. The BTB/POZ and zinc finger domains were almost completely conserved between human and murine BAZF. Fluorescence in situ hybridization analysis revealed that the human BAZF gene is located on chromosome 17p13.1. Although expression of human BAZF mRNA was ubiquitously detected in human tissues, abundant expression was detected in heart and placenta. BAZF mRNA was expressed in some immature B cell lines and erythroleukemia cell lines. The expression in a human erythroleukemia cell line, HEL cells, was upregulated during megakaryocytic differentiation induced by 12-O-tetradecanoyl-phorbol-13-acetate. These expression patterns of BAZF mRNA suggest that BAZF may regulate differentiation in stages or lineages that are different from those regulated by BCL6.
Sakashita
Chizuko
C
Department of Hematology and Oncology, Tokyo Medical and Dental University, Tokyo, Japan.
Fukuda
Tetsuya
T
Okabe
Shinichiro
S
Kobayashi
Hirofumi
H
Hirosawa
Shinsaku
S
Tokuhisa
Takeshi
T
Miyasaka
Nobuyuki
N
Miura
Osamu
O
Miki
Tohru
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17 / Q000737:chemistry; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D008533:Megakaryocytes / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051560:Proto-Oncogene Proteins c-bcl-6; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
C486398:BCL6B protein, human; C113027:Bcl6b protein, mouse; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors