[Development of biosensors for phenol determination from bacteria found in petroleum fields of West Siberia].
Nine Gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation. The plasmid-free derivative of this strain, 32-I-1, did not grow on phenol. The possibility of creating a model biosensor for phenol based on the plasmid-containing 32-I strain is considered. The detection limit for phenol was 5 microM. The optimum conditions for the sensor operation are: pH 7.4, 35 degrees C, and operation time 30 h.
Makarenko
A A
AA
Pushchino State University, Pushchino, Moscow Oblast, 142290 Russia.
Bezverbnaia
I P
IP
Kosheleva
I A
IA
Kuvichkina
T N
TN
Il'iasov
P V
PV
Reshetilov
A N
AN
D015374:Biosensing Techniques; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010578:Petroleum / Q000382:microbiology*; D019800:Phenol / Q000032:analysis* / Q000378:metabolism; D010957:Plasmids / Q000302:isolation & purification; D012800:Siberia; D012988:Soil Microbiology; D013696:Temperature; D013997:Time Factors
D010578:Petroleum; D019800:Phenol