Purification of Food Color Red No. 106 (acid red) using pH-zone-refining counter-current chromatography.
pH-Zone-refining counter-current chromatography was successfully applied to the separation of the main components of Food Color Red No. 106 (R-106, acid red, Color Index No. 45100). A 300-mg quantity of sample was separated using the following two-phase solvent system: n-butanol-water, 40 mM sulfuric acid in organic stationary phase and 30 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and fast atom bombardment mass spectrometry. The separation yielded 261.9 mg of main component of acid red with purity of 99.9%.
Oka
Hisao
H
Aichi Prefectural Institute of Public Health, Nagoya, Japan. hisaooka@alles.or.jp
Suzuki
Masanao
M
Harada
Ken-Ichi
K
Iwaya
Masato
M
Fujii
Kiyonaga
K
Goto
Tomomi
T
Ito
Yuko
Y
Matsumoto
Hiroshi
H
Ito
Yoichiro
Y
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012235:Rhodamines / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D012235:Rhodamines; C022027:lissamine rhodamine B