[The effect of antibiotic therapy on lung pathology in experimental models of sepsis].
OBJECTIVE
In this study, the effect of an extra-lung sepsis model on lung histopathology is evaluated.


METHODS
In this study 20 Wistar-Albino rats were used. Following the ether anesthesia laparotomy was done. Caecum was ligated by a silk thread and was perforated by 18 gauge needle. It is squeezed until feces emerged. Abdominal wall is closed. By this method peritoneal sepsis was performed. The rats are divided into two groups (n:10). 0.5 ml of serum physiologic is applied to the control group, and imipenem is applied to the antibiotic group as 15 mg/kg/tid. 48 hours later rats were sacrificed by extreme ether anesthesia. Relaparatomy was done and diaphragm was open. Multiple biopsies were made from the lung. Biopsy materials was cultured and examined histopathologically.


RESULTS
In control group; rats died in 48 hours (%100), but antibiotic group were alive (%0). The results of lung biopsy cultures are; in all rats in control group, Escherichia coli (E. coli) and Bacteroides fragilis (B. fragilis) were cultured (%100). Whereas in antibiotic group there is no bacteria cultured (p < 0.001). Histopathologic results are: in control group there was wide spread edema and congestion and inflammatory reaction. In antibiotic group there was slight edema, congestion and inflammatory reaction.


CONCLUSIONS
In septic condition sings of adult respiratory distress syndrome occurs. Large spectrum antibiotics can prevent bacterial translocation in lungs and could minimize the lung injury.
Mihmanli
Aydanur
A
Tahaoğlu
Kemal
K
Sahin
Ilyas
I
Ağca
Birol
B
Kalyoncu
Ali
A
Tuncer
Feyzullah
F
Sakiz
Damlanur
D
D000818:Animals; D001441:Bacteroides fragilis / Q000302:isolation & purification; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D015378:Imipenem / Q000627:therapeutic use*; D008171:Lung Diseases / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D011379:Prognosis; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D013845:Thienamycins / Q000627:therapeutic use*
D013845:Thienamycins; D015378:Imipenem