title: Genetic analysis of the liver putative tumor suppressor (LPTS) gene in hepatocellular carcinomas.
abstract: Recently, a novel liver-related putative tumor suppressor (LPTS), which has a growth inhibitory function in the hepatocellular carcinoma (HCC) cell line, has been identified at chromosome 8p23. To determine the relationship of the LPTS with the development or progression of HCC, we analyzed the genetic alterations and the expression pattern of the LPTS gene in a series of 80 HCCs, six dysplastic nodules, and eight large regenerating nodules, determining the genomic structures. We identified a total of seven exons, of which two were alternative, and three LPTS isoforms, short (LPTS-S), medium (LPTS-M), and long-sizes (LPTS-L). In the genetic alteration study of the LPTS gene, no mutation was detected in the large regenerating nodules, dysplastic nodules, and HCC, whereas ten (34.5%) of 29 informative cases at one or more intragenic polymorphic sites showed loss of heterozygosity (LOH). Interestingly, LOH was identified only in HCC samples with hepatitis B virus (HBV) infection and the frequency of LOH was not statistically related with histologic grade and clinical stage, suggesting that allelic loss of the LPTS gene may occur as an early event in the development of HCC, especially in the cases with HBV infection.
authors.affiliation: Department of Pathology, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, South Korea.
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.forename: Jong Heun
authors.initials: JH
authors.lastname: Lee
authors.forename: Jik Young
authors.initials: JY
authors.lastname: Park
authors.forename: Seong Whan
authors.initials: SW
authors.lastname: Jeong
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Shin
authors.forename: Hong Sug
authors.initials: HS
authors.lastname: Kim
authors.forename: Sang Kyu
authors.initials: SK
authors.lastname: Lee
authors.forename: Shi Nae
authors.initials: SN
authors.lastname: Lee
authors.forename: Sug Hyung
authors.initials: SH
authors.lastname: Lee
authors.forename: Chul Geun
authors.initials: CG
authors.lastname: Park
authors.forename: Nam Jin
authors.initials: NJ
authors.lastname: Yoo
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D005091:Exons / Q000235:genetics; D005260:Female; D016147:Genes, Tumor Suppressor; D006509:Hepatitis B / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D025521:Tumor Suppressor Proteins
chemical_list: D018797:Cell Cycle Proteins; C440808:PINX1 protein, human; D011506:Proteins; D025521:Tumor Suppressor Proteins