[An experimental research of taurine on H2O2-induced bovine lens epithelial cell apoptosis].
OBJECTIVE
To study the inhibitory role of taurine on H(2)O(2)-induced bovine lens epithelial cell apoptosis.


METHODS
By terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) detection and bovine lens organ culture, we examined the affect of taurine on the number of H(2)O(2)-induced bovine lens epithelial cells with apoptosis, the related comparisons and statistic analyses were carried out.


RESULTS
(1) Lens epithelial cell apoptosis began before lens opaqueness (lens became opaque after 6 hours of incubation, while the lens epithelial cell apoptosis was detected after 3 hours of incubation); (2) Taurine could apparently inhibit H(2)O(2)-induced bovine lens epithelial cell apoptosis.


CONCLUSIONS
Taurine has an inhibitory role on H(2)O(2)-induced bovine lens epithelial cell apoptosis and can delay and ameliorate the occurrence and development of cataract.
Chen
F
F
Beijing Institute of Ophthalmology, Beijing 100005, China.
Chen
C
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D006861:Hydrogen Peroxide / Q000009:adverse effects; D007908:Lens, Crystalline / Q000473:pathology*; D013654:Taurine / Q000494:pharmacology*
D013654:Taurine; D006861:Hydrogen Peroxide