title: [The mechanism of p16 gene inactivation in human bladder transitional cell carcinoma].
abstract: OBJECTIVE
  To figure out the mechanism of p16 gene inactivation in human bladder transitional cell carcinoma (BTCC).
  
  
  METHODS
  p16 gene was analyzed for genetic alterations by Southern blot analysis, PCR-SSCP and DNA methylation analysis in 52 cases of transitional cell carcinoma of bladder.
  
  
  RESULTS
  p16 gene deletion was found in 11 of 52 (21.15%) BTCCs, only one tumor showed point mutation on p16 exon 2, nineteen cases of BTCCs (36.53%) showed p16 gene 5' CpG island methylation.
  
  
  CONCLUSIONS
  There was a statistically significant association between p16 gene deletion and early stage, well differentiated tumors. Additionally, a statistically significant correlation was also noticed between p16 gene 5' CpG island methylation and tumors in late stage with poor cell differentiation.
authors.affiliation: Tianjin Institute of Urological Surgery, Tianjin 300211.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: T
authors.initials: T
authors.lastname: Ma
authors.forename: C
authors.initials: C
authors.lastname: Wu
mesh_terms: D015139:Blotting, Southern; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000473:pathology*; D018899:CpG Islands / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D020868:Gene Silencing; D006801:Humans; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm