[Anti-CD40 McAb induces CD40+ malignant B cell proliferation arrest and apoptosis].
OBJECTIVE
To study the expression of CD(40) molecule and the biological effects mediated by CD(40) molecules on malignant B cells.


METHODS
Agonistic anti-human CD(40) monoclonal antibody (5C11) was added to the cell culture system. Cell counting, PI staining, Annexin-V staining and flow cytometric analysis were used to study the biological effects of 5C11 on malignant B cell lines.


RESULTS
5C11 induced homotypic aggregation, proliferation arrest and apoptosis in the multiple myeloma cell line XG2 highly expressed CD(40). 5C11 induced B lymphoma cell line Daudi homotypic aggregation and proliferation arrest but no apoptosis.


CONCLUSIONS
5C11 can inhibit proliferation of malignant B cells by inducing apoptosis or arresting the cells at G(2)/M interphase.
Zhou
Z
Z
Department of Immunology, Suzhou Medical College, Suzhou 215007, China.
Shi
Q
Q
Wang
J
J
Pan
J
J
Zhang
X
X
D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D017209:Apoptosis; D001402:B-Lymphocytes / Q000502:physiology*; D019013:CD40 Antigens / Q000502:physiology*; D002449:Cell Aggregation; D002455:Cell Division; D006801:Humans; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000628:therapy*; D009101:Multiple Myeloma / Q000628:therapy*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019013:CD40 Antigens