Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation.
OBJECTIVE
To study the effects of praeruptorin C (pra-C) on proliferation of cattle aortic smooth muscle cells (SMC).


METHODS
The DNA synthesis of SMC was measured using the incorporation of [3H]thymidine([3H]TdR). Cell cycle phase was evaluated by flow cytometry and cytotoxicity was evaluated by measuring lactic dehydrogenase (LDH) activity.


RESULTS
Whether or not treated with angiotensin II (Ang-II), SMC proliferation was suppressed by pra-C in a concentration-dependent manner at range from 0.001 micromol/L to 10 micromol/L. The inhibitory effects appeared to be related to G1-S block in cell cycle traverse while the LDH activities did not change dramatically.


CONCLUSIONS
Pra-C can completely inhibit SMC proliferation induced by Ang II and partly inhibit the growth of SMC- induced by bovine serum, which is important in prevention and treatment of vascular hyperplastic disease.
Wei
En-Hui
EH
Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China.
Rao
Man-Ren
MR
Chen
Xiu-Ying
XY
Fan
Le-Ming
LM
Chen
Qi
Q
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001013:Aorta, Thoracic / Q000166:cytology; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D003374:Coumarins / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D000804:Angiotensin II; C058808:praeruptorin C; D004247:DNA; D007770:L-Lactate Dehydrogenase