Carbohydrate microarrays for the recognition of cross-reactive molecular markers of microbes and host cells.
We describe here the development of a carbohydrate-based microarray to extend the scope of biomedical research on carbohydrate-mediated molecular recognition and anti-infection responses. We have demonstrated that microbial polysaccharides can be immobilized on a surface-modified glass slide without chemical conjugation. With this procedure, a large repertoire of microbial antigens (approximately 20,000 spots) can be patterned on a single micro-glass slide, reaching the capacity to include most common pathogens. Glycoconjugates of different structural characteristics are shown here to be applicable for microarray fabrication, extending the repertoires of diversity and complexity of carbohydrate microarrays. The printed microarrays can be air-dried and stably stored at room temperature for long periods of time. In addition, the system is highly sensitive, allowing simultaneous detection of a broad spectrum of antibody specificities with as little as a few microliters of serum specimen. Finally, the potential of carbohydrate microarrays is demonstrated by the discovery of previously undescribed cellular markers, Dex-Ids.
Wang
Denong
D
Functional Genomics Division, Columbia Genome Center, College of Physicians and Surgeons, Columbia University, 1150 St. Nicholas Avenue, New York, NY 10032, USA. dw8@columbia.edu
Liu
Shaoyi
S
Trummer
Brian J
BJ
Deng
Chao
C
Wang
Aili
A
D000818:Animals; D001709:Biotechnology / Q000379:methods; D002241:Carbohydrates / Q000737:chemistry*; D003101:Collodion / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007444:Inulin / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011134:Polysaccharides / Q000737:chemistry; D013696:Temperature
D002241:Carbohydrates; D003911:Dextrans; D011134:Polysaccharides; D003101:Collodion; D007444:Inulin