Chromosomal mapping of HCaRG, a novel hypertension-related, calcium-regulated gene.
We recently identified a novel gene that is negatively regulated by extracellular calcium concentration with higher levels of transcripts in hypertensive animals (SHR). We named this gene HCaRG (Hypertension-related, Calcium-Regulated Gene). In this work we report the chromosomal localization of the HCaRG gene among different species. We identified a BglII RFLP between BN.lx and SHR rats. We then analysed the strain distribution pattern of this RFLP in 31 RIS, originating from BN.lx and SHR rats, and compared it to the segregation of 475 markers localized in the rat genetic map. Hcarg localizes to the rat chromosome 7 between the markers Mit3 and Mit4. This region is homologous to human chromosome 8q21-24. We identified three clones in Genbank that contain the sequence of HCaRG. It was therefore possible to narrow down the localization of human HCaRG to chromosome 8q24.3. Furthermore, a suggestive localization of mouse Hcarg based on conservation of linkage between human and mouse is on chromosome 15. We previously identified a putative calcium-binding motif (EF-Hand) and a nuclear receptor-binding domain (LxxLL) in the rat sequence of the HCaRG protein. Sequence comparison between five different species showed that these domains are highly conserved. Furthermore, a search of ESTs in Genbank for homologous sequences showed that HCaRG is expressed only in eukaryotes, particularly in mammals.
Solban
N
N
Centre Hospitalier de l'Université de Montréal, Québec, Canada.
Dumas
P
P
Gossard
F
F
Sun
Y
Y
Pravenec
M
M
Kren
V
V
Lewanczuk
R
R
Hamet
P
P
Tremblay
J
J
D048868:Adaptor Proteins, Signal Transducing; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001847:Bone Diseases / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats / Q000235:genetics*; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013552:Swine
D048868:Adaptor Proteins, Signal Transducing; C485926:COMMD5 protein, human; D018797:Cell Cycle Proteins; C415469:Commd5 protein, rat; D009687:Nuclear Proteins; D002118:Calcium