A high-pressure liquid chromatographic-tandem mass spectrometric method for the determination of ethambutol in human plasma, bronchoalveolar lavage fluid, and alveolar cells.
A technique is presented for the specific and sensitive determination of ethambutol concentrations in plasma, bronchoalveolar lavage (BAL), and alveolar cells (AC) using a high-pressure liquid chromatographic (HPLC)-tandem mass spectrometric (MS-MS) method. The preparation of samples requires a deproteinization step with acetonitrile. The retention times for ethambutol, neostigmine bromide, and propranolol are 2.0, 1.4, and 1.1 min, respectively, with a total run time of 2.8 min. The detection limits for ethambutol are 0.05 microg/mL for plasma and 0.005 microg/mL for the BAL supernatants and AC suspensions. The assay has excellent performance characteristics and has been used to support a study of the intrapulmonary pharmacokinetics of ethambutol in human subjects.
Conte
John E
JE
Department of Epidemiology and Biostatistics, Infectious Diseases Research Laboratory, University of California, San Francisco, 94117, USA.
Lin
Emil
E
Zhao
Yeping
Y
Zurlinden
Elisabeth
E
D000995:Antitubercular Agents / Q000032:analysis* / Q000097:blood; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004977:Ethambutol / Q000032:analysis* / Q000097:blood; D013058:Mass Spectrometry / Q000379:methods*; D011650:Pulmonary Alveoli / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000995:Antitubercular Agents; D004977:Ethambutol