Chromosome aberrations detected by FISH and correlation with cell survival after irradiation at various dose-rates and after bromodeoxyuridine radiosensitization.
OBJECTIVE
To determine whether measurement of chromosome aberrations by fluorescence in situ hybridization (FISH) predicts cell survival after irradiation at different dose-rates and after radiosensitization by bromodeoxyurdine (BrdU) in a lung carcinoma cell line.


METHODS
The human lung carcinoma cell line SW1573 was irradiated at high dose-rate (HDR: 0.8 Gy min-1) or at pulsed low dose-rate (p-LDR: average dose-rate 1 Gy h-1) with or without radiosensitization by bromodeoxyuridine (BrdU). Cell survival was determined by clonogenic assay. Chromosome aberrations (colour junctions) were measured by whole-chromosome FISH of chromosome 2 and 18 and were scored according to the PAINT method.


RESULTS
Clear radiosensitization by BrdU was observed both after HDR and p-LDR irradiation. Chromosome 18 was more radiosensitive than chromosome 2. There was a good correlation between induction of colour junctions and cell survival both after HDR and p-LDR irradiation and after radiosensitization by BrdU.


CONCLUSIONS
Determination of chromosome aberrations by FISH can predict cell survival after different dose-rates and after radiosensitization by BrdU
Castro Kreder
N
N
Academic Medical Center, University of Amsterdam, Department of Radiotherapy, PO BOX 22700, 1100 DE Amsterdam, The Netherlands. N.Castro@amc.uva.nl
Van Bree
C
C
Franken
N A P
NA
Haveman
J
J
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011836:Radiation Tolerance / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D001973:Bromodeoxyuridine