Adoptive protection from experimental myasthenia gravis with T cells from mice treated nasally with acetylcholine receptor epitopes.
Nasal administration of synthetic CD4(+) epitopes of the acetylcholine receptor (AChR) prevents experimental myasthenia gravis (EMG) in C57Bl/6 mice, but not in IL4-deficient C57Bl/6 (IL4(-/-)) mice. Here we verify that nasal tolerance requires IL4, by showing that CD4(+) cells from C57Bl/6 mice treated nasally with a pool of AChR CD4(+) epitopes protected IL4(-/-) mice from EMG and caused a reduced production of anti-AChR antibody. CD4(+) cells from C57Bl/6 mice treated with unrelated peptides or sham-treated did not induce protection. CD4(+) cells from C57Bl/6 mice treated with just one AChR peptide protected IL4(-/-) mice from EMG without affecting antibody synthesis.
Monfardini
Cristina
C
Department of Biochemistry, Molecular Biology and Biophysics, College of Biological Sciences, University of Minnesota, St. Paul, MN 55108, USA.
Milani
Monica
M
Ostlie
Norma
N
Wang
Wei
W
Karachunski
Peter I
PI
Okita
David K
DK
Lindstrom
Jon
J
Conti-Fine
Bianca M
BM
D000281:Administration, Intranasal; D019264:Adoptive Transfer; D000818:Animals; D000906:Antibodies / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000145:classification; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000517:prevention & control*; D011950:Receptors, Cholinergic / Q000032:analysis / Q000276:immunology*
D000906:Antibodies; D018984:Epitopes, T-Lymphocyte; D007074:Immunoglobulin G; D011950:Receptors, Cholinergic; D015847:Interleukin-4; D007371:Interferon-gamma