title: Hepatic iron accumulation is not directly associated with induction of DNA strand breaks in the liver cells of Long-Evans Cinnamon (LEC) rats.
abstract: Effects of accumulation of copper and iron on induction of DNA strand breaks were investigated in Long-Evans Cinnamon (LEC) rats that spontaneously develop fulminant hepatitis. Copper and iron accumulated in the liver of LEC rats in an age-dependent manner from 4 to 15 weeks. Low-iron diet prevented the accumulation of iron in the liver, but did not prevent accumulation of copper. The amounts of DNA strand breaks that were estimated by comet assay in the liver cells of rats fed standard diet increased with age from 4 to 15 weeks. No significant differences were observed in the proportions of LEC rat liver cells without tail and the average lengths of tail momentum in the comet images between LEC rats that had been fed standard MF diet and low-iron diet. These results support the idea that accumulation of iron is not directly associated with the induction of DNA damage in the liver cells of LEC rats.
authors.affiliation: Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Hayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kuge
authors.forename: Daiji
authors.initials: D
authors.lastname: Endoh
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakayama
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
authors.forename: Akira
authors.initials: A
authors.lastname: Takazawa
authors.forename: Toyo
authors.initials: T
authors.lastname: Okui
mesh_terms: D000375:Aging; D000818:Animals; D003300:Copper / Q000378:metabolism; D004249:DNA Damage; D004032:Diet; D007501:Iron / Q000378:metabolism*; D019266:Iron, Dietary / Q000008:administration & dosage; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D019266:Iron, Dietary; D003300:Copper; D007501:Iron