title: Human prostatic carcinoma cells produce an increase in the synthesis of interleukin-6 by human osteoblasts.
abstract: BACKGROUND
  The aim of this work was to evaluate the effect produced by conditioned medium from human prostatic carcinoma cell (PC-3) culture on human osteoblast (HOB) interleukin 6 (IL-6) synthesis.
  
  
  METHODS
  PC-3 cells were cultured in Ham's F12K medium with 10% fetal calf serum (FCS) up to confluence. Medium was changed by Dulbecco modified Eagle medium (DMEM)/F12K (1:1) with 0.1% bovine serum albumin. Cells were cultured for 24 hr, and medium (PC-3-CM) was collected. HOBs were cultured up to confluence, and after 48 hr without FCS, medium was removed and PC-3-CM was added to the wells. After 24 hr, supernatant was collected for the determination of IL-6. In another experiment, HOBs were cultured up to confluence in Petri dishes, and after 48 hr without FCS, PC-3-CM or DMEM/F12K (1:1) was added. After different periods of time, medium was removed, and total RNA was extracted. IL-6 mRNA was quantified using reverse transcription polymerase chain reaction.
  
  
  RESULTS
  PC-3-CM significantly enhanced IL-6 secretion into HOB culture supernatants (between 1,812% and 372%, depending on the osteoblastic line) with respect to HOBs cultured in DMEM/F12K. PC-3-CM also produced an increase in IL-6 mRNA levels in HOBs.
  
  
  CONCLUSIONS
  Prostate carcinoma cells (PC-3) produce a factor or factors that enhance the synthesis and release of IL-6, a known activator of bone resorption.
authors.affiliation: Section of Bone Pathophysiology, Department of Biochemistry, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: García-Moreno
authors.forename: Cioly
authors.initials: C
authors.lastname: Méndez-Dávila
authors.forename: Concepción
authors.initials: C
authors.lastname: de La Piedra
authors.forename: Nilda Adriana
authors.initials: NA
authors.lastname: Castro-Errecaborde
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Traba
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D002450:Cell Communication / Q000502:physiology*; D017077:Culture Media, Conditioned; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D015850:Interleukin-6; D012333:RNA, Messenger