title: Residue depletion of thiamphenicol in the sea-bass.
abstract: The residue depletion of thiamphenicol (TAP) was investigated in the sea-bass (Dicentrarchus labrax) after 5 days' treatment with medicated food at a dose of 15 or 30 mg/kg bw/day. Fish were sampled for blood and muscle + skin from 3 h until 14 days after treatment. Thiamphenicol concentrations were assayed by high performance liquid chromatography. Thiamphenicol concentrations measured 3 h after stopping treatment were 0.77 microg/mL and 0.91 (15 mg/kg dose) or 1.32 microg/mL and 1.47 microg/g (30 mg/kg dose), in plasma and muscle + skin, respectively. After a withdrawal of 3 days, plasma and tissue concentrations were: 0.08 microg/mL and 0.03 microg/g (lower dose) or 0.12 microg/mL and 0.06 microg/g (higher dose), respectively. Thiamphenicol was not detectable either in plasma or in tissues on days 7, 10 and 14 following withdrawal of the medicated food. Based on maximum residue levels (MRL) for TAP in fin fish, established at 50 microg/kg for muscle and skin in natural proportions, a withdrawal period of 5 and 6 days is proposed, after treatment at 15 or 30 mg/kg of TAP with medicated feed pellets, respectively, to avoid the presence of violative residues in the edible tissues of the sea-bass.
authors.affiliation: Department of Veterinary Clinics, Section of Pharmacology and Toxicology, University of Pisa, Pisa, Italy. intorre@vet.unipi.it
authors.forename: L
authors.initials: L
authors.lastname: Intorre
authors.forename: G
authors.initials: G
authors.lastname: Castells
authors.forename: C
authors.initials: C
authors.lastname: Cristòfol
authors.forename: S
authors.initials: S
authors.lastname: Bertini
authors.forename: G
authors.initials: G
authors.lastname: Soldani
authors.forename: M
authors.initials: M
authors.lastname: Arboix
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D001492:Bass / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004350:Drug Residues / Q000493:pharmacokinetics; D013839:Thiamphenicol / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D013839:Thiamphenicol