[Correlation of cell apoptosis induction with expression of human beta5 integrin on hematopoietic cells].
OBJECTIVE
To investigate the function of alpha(v)beta5 integrin in hematopoietic cells.


METHODS
Beta5 integrin cDNA was expressed in K562 cells through a retroviral vector system. The changes of the alpha(v)beta5 and alpha(v)beta3 integrins expression in apoptosis and differentiation induced by serum depletion were observed.


RESULTS
The beta5 integrin cDNA failed to express in K562 cells after the introduction of the beta5 integrin retrovirus vector pG beta5CHT. Imbalanced expression of alpha(v)beta3 and alpha(v)beta5 integrins occurred during apoptosis and differentiation induced by serum depletion. Treatment of hematopoietic cells with anti-alpha(v)beta5 monoclonal antibody inhibited the cells apoptosis.


CONCLUSIONS
Overexpression of the alpha(v)beta5 integrin cDNA in hematopoietic cells was associated with the inhibition of cell proliferation and apoptosis.
Yin
L
L
Department of Pathophysiology, Shanghai Medical University, Shanghai 200032, China.
Zhao
X
X
Fu
S
S
Garacia-Sanchez
F
F
Deisseroth
A B
AB
D017209:Apoptosis; D049109:Cell Proliferation; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D039641:Integrin beta Chains / Q000235:genetics* / Q000378:metabolism; D020014:K562 Cells
D039641:Integrin beta Chains; C084344:integrin beta5