title: Establishment and characterization of a cell line from a malignant fibrous histiocytoma of bone developing in a patient with multiple fibrous dysplasia.
abstract: OBJECTIVE
  In order to provide material for genetic analysis of fibrous dysplasia (FD), a cell line designated GBS-1 was established from a secondary bone malignant fibrous histiocytoma (MFH) developing in a rib of a 44-year-old male polyostotic FD patient.
  
  
  RESULTS
  The GBS-1 cells are characterized by a pleomorphic spindle cell morphology with abundant mucus production. On transplantation to nude mouse subcutis the cell line forms myxoid-spindle cell sarcomas with giant cells, the myxoid product being positive for periodic acid-Schiff (PAS) and alcian blue (Al-B) stains and completely digested by hyaluronidase, mimicking the original tumor. Chromosome and genetic analyses revealed multiple structural and numerical abnormalities of chromosomes with a large number of unidentifiable chromosomes and p53 mutation in exon 7 with LOH in the counterpart.
  
  
  CONCLUSIONS
  Since cell lines for FD have hitherto not been available, the GBS-1 cells should prove useful for genetic analyses of FD and also MFH of bone origin.
authors.affiliation: Department of Pathology, The Cancer Institute of the Japanese Foundation for Cancer Research, Tokyo, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Fang
authors.forename: H
authors.initials: H
authors.lastname: Mukai
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.forename: K
authors.initials: K
authors.lastname: Shinomiya
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: N
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
authors.forename: H
authors.initials: H
authors.lastname: Kanda
mesh_terms: D000328:Adult; D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002874:Chromosome Mapping; D018450:Disease Progression; D005357:Fibrous Dysplasia of Bone / Q000473:pathology*; D016158:Genes, p53; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000473:pathology*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured