[Dynamic expression of tenascin in rat liver during liver fibrogenesis induced by CCl(4)].
OBJECTIVE
To study the expression of tenascin in normal and fibrotic rat liver.


METHODS
Liver fibrosis induced in rat with CCl(4) were divided into three stages: the stage of hepatic injury (4 weeks), early stage of hepatic fibrosis (8 weeks) and later stage of hepatic fibrosis (12 weeks). Tenascin expression in liver tissue was observed by immunohistochemical method and in situ hybridization using digoxigenin-labelled DNA probe.


RESULTS
In normal rat liver there was a weak staining for tenascin. In both liver injury stage and early stage of hepatic fibrosis, both mRNA signal and immunostaining for tenascin were significantly increased as compared to that in normal liver. In later stage of hepatic fibrosis, mRNA signal and immunostaining for tenascin were decreased compared with that in early stage of hepatic fibrosis. The cellular source of tenascin in liver mainly restricted in mesenchymal cells.


CONCLUSIONS
Tenascin is a component of the extracellular matrix of liver tissue. Plays a role in early matrix organization during liver fibrogenesis.
Yao
Dingkang
D
Department of Gastroenterology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
Li
Shi
S
Kong
Xiantao
X
Ye
Tingjun
T
Fan
Jiangao
J
Zhong
Lan
L
Wang
Guoliang
G
Tian
Liyan
L
Wu
Wensen
W
Li
Mingsheng
M
D000818:Animals; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D019063:Tenascin / Q000096:biosynthesis* / Q000235:genetics
D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D019063:Tenascin; D002251:Carbon Tetrachloride