Increase of serum phosphatidylcholine hydroperoxide dependent on glycemic control in type 2 diabetic patients.
In order to clarify the relationship between serum phosphatidylcholine hydroperoxide (PCOOH) levels and blood glucose control in type 2 diabetes patients (DM), DM (n = 61) and normal control (n = 11) were enrolled. High-density lipoprotein (HDL) was separated from serum by the addition of sodium phosphotungstate and magnesium chloride, and the precipitated fraction was prepared as non-HDL. Phospholipids were extracted from whole serum, non-HDL and HDL to estimate PCOOH level with chemiluminescence high performance liquid chromatography (CL-HPLC). PCOOH level (nmol/l, mean +/- S.D.) was higher in DM than in control (33.1 +/- 9.5 vs. 23.0 +/- 8.2 for serum; P < 0.01, 17.0 +/- 5.5 vs. 10.6 +/- 3.8 for non-HDL; P < 0.01, and 16.1 +/- 6.3 vs. 12.3 +/- 5.5 for HDL; not significant, respectively). DM was divided into five groups according to hemoglobin A(1c) (HbA(1c)) levels (%): (1) less than 6, (2) 6-6.4, (3) 6.5-6.9 (4) 7.0-7.4, and (5) over than 7.5. Increase of PCOOH levels was dependent on HbA(1c). We concluded that (1) serum and non-HDL PCOOH increased in DM, (2) the level was strongly correlated with diabetic control, and (3) approximately a half amount of serum PCOOH was present in HDL of both control and DM.
Nagashima
Tazuko
T
CL Research Laboratory, Tohoku Electronic Industrial Co., Ltd., Sendai, Japan.
Oikawa
Shinichi
S
Hirayama
Yoshitake
Y
Tokita
Yoshihisa
Y
Sekikawa
Akihiro
A
Ishigaki
Yasushi
Y
Yamada
Rie
R
Miyazawa
Teruo
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000378:metabolism*; D001794:Blood Pressure; D002851:Chromatography, High Pressure Liquid; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D006442:Glycated Hemoglobin; D008075:Lipoproteins, HDL; D010713:Phosphatidylcholines; D010743:Phospholipids; D014280:Triglycerides; C057590:phosphatidylcholine hydroperoxide