Separation of tanshinones from Salvia miltiorrhiza bunge by multidimensional counter-current chromatography.
Analytical and preparative high-speed counter-current chromatography (HSCCC) was successfully used for the isolation and purification of tanshinones from the roots of Salvia miltiorrhiza Bunge. Using multidimensional HSCCC, four major components including tanshinone IIA (16 mg), tanshinone I (10 mg), dihydrotanshinone I (7 mg) and cryptotanshinone (11 mg) were isolated each at high purity of over 95%.
Tian
Guilian
G
Beijing Institute of New Technology Application, PR China.
Zhang
Tianyou
T
Zhang
Yabin
Y
Ito
Yoichiro
Y
D045784:Abietanes; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D010616:Phenanthrenes / Q000302:isolation & purification*; D027544:Salvia / Q000737:chemistry*
D045784:Abietanes; D010616:Phenanthrenes; C021751:tanshinone