[Detection and analysis of SYT-SSX fusion gene in synovial sarcoma].
OBJECTIVE
To demonstrate that a characteristic SYT-SSX fusion gene resulting from chromosomal translocation t (X; 18) (p11; q11) was detectable in archival paraffin-embedded synovial sarcoma tissues.


METHODS
SYT-SSX fusion transcripts in 20 synovial sarcomas (histologic subtypes, 15 monophasic and 5 biphasic) were detected and analyzed by the reverse-transcriptase polymerase chain reaction and compared with the results from relevant pathological data.


RESULTS
A specific SYT-SSX RT-PCR product was found in 19 of 20 (95%) synovial sarcomas tested and of the 13 tumors containing SYT-SSX2, 10 were monophasic.


CONCLUSIONS
SYT-SSX fusion transcripts are considered as a defining diagnostic marker of synovial sarcomas and the subtypes of SYT-SSX fusion transcripts (SYT-SSX1 and SYT-SSX2) may yield prognostic information.
Xu
Z
Z
Department of Pathology, Health Science Center, Peking University, Beijing 100083, China.
Yang
T
T
Wu
B
B
Zhong
H
H
Yang
Z
Z
Zhou
B
B
Zhang
C
C
Zheng
J
J
D000293:Adolescent; D000328:Adult; D019677:Artificial Gene Fusion; D002648:Child; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013584:Sarcoma, Synovial / Q000235:genetics* / Q000473:pathology
D015514:Oncogene Proteins, Fusion; D012333:RNA, Messenger; C116512:SYT-SSX fusion protein