Selective induction of mitogen-activated protein kinases in human lens epithelial cells by ultraviolet radiation.
The present study investigates the effects of ultraviolet radiation (UVR) on mitogen-activated protein kinase (MAPK) activity in human lens epithelial (HLE) cells. Irradiation of HLE cells with ultraviolet B and ultraviolet C radiation activates the stress-response MAPK proteins, p38 and c-Jun NH(2)-terminal kinase (JNK), in a dose- and time-dependent manner, while the extracellular-regulated signal kinase (ERK 44/42) cascade was not altered by UVR exposure. Ultraviolet A radiation failed to elicit a MAPK response. UVR-induced MAPK activation does not require protein kinase C or phosphatidylinositol 3-kinase activity, suggesting that this is not a receptor-mediated event. Inhibition of ribosomal translation completely abolished UVR-induced MAPK activation, while treatment with the antioxidant, N-acetyl cysteine, and mild heat shock had no effect on this activation. These data demonstrate for the first time the selective activation of MAPK cascades in a lens epithelial cell line.
Bomser
Joshua A
JA
Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, USA. bomser.1@osu.edu
D015153:Blotting, Western; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004790:Enzyme Induction / Q000528:radiation effects; D004847:Epithelial Cells / Q000201:enzymology* / Q000528:radiation effects*; D006358:Hot Temperature; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D048031:JNK Mitogen-Activated Protein Kinases; D007908:Lens, Crystalline / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects*; D048670:MAP Kinase Kinase 4; D020935:MAP Kinase Signaling System / Q000528:radiation effects; D008301:Maleimides / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000096:biosynthesis; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis*; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014466:Ultraviolet Rays
D007211:Indoles; D008301:Maleimides; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D020929:Mitogen-Activated Protein Kinase Kinases; C070515:bisindolylmaleimide I; C064758:Ro 31-8220