PATE, a gene expressed in prostate cancer, normal prostate, and testis, identified by a functional genomic approach.
To identify target antigens for prostate cancer therapy, we have combined computer-based screening of the human expressed sequence tag database and experimental expression analysis to identify genes that are expressed in normal prostate and prostate cancer but not in essential human tissues. Using this approach, we identified a gene that is expressed specifically in prostate cancer, normal prostate, and testis. The gene has a 1.5-kb transcript that encodes a protein of 14 kDa. We named this gene PATE (expressed in prostate and testis). In situ hybridization shows that PATE mRNA is expressed in the epithelial cells of prostate cancers and in normal prostate. Transfection of the PATE cDNA with a Myc epitope tag into NIH 3T3 cells and subsequent cell fractionation analysis shows that the PATE protein is localized in the membrane fraction of the cell. Analysis of the amino acid sequence of PATE shows that it has structural similarities to a group of proteins known as three-finger toxins, which includes the extracellular domain of the type beta transforming growth factor receptor. Restricted expression of PATE makes it a potential candidate for the immunotherapy of prostate cancer.
Bera
Tapan K
TK
Laboratory of Molecular Biology and Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Maitra
Rangan
R
Iavarone
Carlo
C
Salvatore
Giuliana
G
Kumar
Vasantha
V
Vincent
James J
JJ
Sathyanarayana
B K
BK
Duray
Paul
P
Lee
B K
BK
Pastan
Ira
I
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016208:Databases, Factual; D004847:Epithelial Cells; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger; D013737:Testis / Q000378:metabolism*
D008565:Membrane Proteins; C452641:PATE1 protein, human; D012333:RNA, Messenger