title: Finding your knockout: reverse genetics techniques for plants.
abstract: The process of finding a mutant plant for your gene of interest has recently become far more straightforward and painless than has ever been possible before. This has come about through the production of large-scale insertional mutagenesis populations. These can now be readily screened in bulk for insertional mutants through a mixture of molecular (PCR/hybridization) and bioinformation techniques. Here we describe a step-by-step guide to the molecular protocols and a description of the bioinformatics approach.
authors.affiliation: Nottingham Arabidopsis Stock Centre, School of Biosciences, Plant Science Division, University of Nottingham, UK. Sean_May@nasc.nott.ac.uk
authors.forename: Sean T
authors.initials: ST
authors.lastname: May
authors.forename: Deborah
authors.initials: D
authors.lastname: Clements
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
mesh_terms: D000465:Algorithms; D017360:Arabidopsis / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D017343:Genes, Plant / Q000502:physiology*; D005821:Genetic Techniques; D008958:Models, Molecular; D016254:Mutagenesis, Insertional / Q000235:genetics / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D030821:Plants, Genetically Modified / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA