[The effect of heparin on the deep second-degree burn in scalded rats].
OBJECTIVE
To investigate the effects of heparin on deep second-degree burn in scalded rats.


METHODS
Thirty Sprague-Dawley rats inflicted with 20% TBSA deep second-degree scalding on the back were employed as the model and were randomly divided into two groups. i,e heparin (H) and normal saline groups (N). Heparin (100 U . kg(-1) . day(-1)) was injected subcutaneously to the rats in H group while normal saline to those in N group once a day. Wound healing rate and immunological indices were determined simultaneously.


RESULTS
(1) The wound healing time was much shorter in H group than that in N group (P < 0.005). The granulation tissue growth condition examined by LM and EM was much better in H group that than in N group. (2) The lymphocytic transforming ratio in H group was evidently increased (P < 0.005). And the phagocytic function of the macrophages increased significantly (P < 0.05). But there was no difference between the two groups in terms of hemoltytic plaque test (P > 0.05).


CONCLUSIONS
Injection of heparin subcutaneously could accelerate burn wound healing and strengthen the systemic cellular immune potential and the macrophage phagocytic capacity of the host, but exerted no effect on the humoral immunity.
Cen
Y
Y
Department of Burns, The First Affiliated Hospital of West China Medical University, Chengdu 630000, Si Chuan Province P.R. China.
Luo
P
P
Yan
X
X
D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006493:Heparin / Q000008:administration & dosage / Q000627:therapeutic use*; D007279:Injections, Subcutaneous; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
D006493:Heparin