title: Towards disposable lab-on-a-chip: poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection.
abstract: A fully disposable microanalytical device based on combination of poly(methylmethacrylate) (PMMA) capillary electrophoresis microchips and thick-film electrochemical detector strips is described. Variables influencing the separation efficiency and amperometric response, including separation voltage or detection potential are assessed and optimized. The versatility, simplicity and low-cost advantages of the new design are coupled to an attractive analytical performance, with good precision (relative standard deviation RSD = 1.68% for n = 10). Applicability for assays of mixtures of hydrazine, phenolic compounds, and catecholamines is demonstrated. Such coupling of low-cost PMMA-based microchips with thick-film electrochemical detectors holds great promise for mass production of single-use micrototal analytical systems.
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. joewang@nmsu.edu
authors.forename: Joseph
authors.initials: J
authors.lastname: Wang
authors.forename: Martin
authors.initials: M
authors.lastname: Pumera
authors.forename: Madhu Prakash
authors.initials: MP
authors.lastname: Chatrathi
authors.forename: Alberto
authors.initials: A
authors.lastname: Escarpa
authors.forename: Renate
authors.initials: R
authors.lastname: Konrad
authors.forename: Anja
authors.initials: A
authors.lastname: Griebel
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Dörner
authors.forename: Holger
authors.initials: H
authors.lastname: Löwe
mesh_terms: D002395:Catecholamines / Q000032:analysis; D004209:Disposable Equipment; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods / Q000592:standards; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D006834:Hydrazines / Q000032:analysis; D008832:Microchemistry / Q000295:instrumentation; D010636:Phenols / Q000032:analysis; D019904:Polymethyl Methacrylate
chemical_list: D002395:Catecholamines; D006834:Hydrazines; D010636:Phenols; C029424:hydrazine; D019904:Polymethyl Methacrylate