Alpha-LNA (locked nucleic acid with alpha-D-configuration): synthesis and selective parallel recognition of RNA.
Alpha-LNA is presented as a stereoisomer of LNA (locked nucleic acid) with alpha-D-configuration. Three different approaches towards the thymine alpha-LNA monomer as well as the 5-methylcytosine alpha-LNA monomer are presented. Different alpha-LNA sequences have been synthesised and their hybridisation with complementary DNA and RNA has been evaluated by means of thermal stability experiments and circular dichroism spectroscopy. In a mixed pyrimidine sequence, alpha-LNA displays unprecedented parallel-stranded and selective RNA binding. Furthermore, a remarkable selectivity for hybridisation with RNA over DNA is indicated.
Nielsen
Poul
P
Department of Chemistry, University of Southern Denmark, Odense M. pon@chem.sdu.dk
Christensen
Nanna K
NK
Dalskov
Jakob K
JK
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004355:Drug Stability; D006358:Hot Temperature; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism
D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA