title: [The adherence of leukocytes mediated by L-selectin to endothelia activated by burn sera].
abstract: OBJECTIVE
  To study the mediating effects of L-selectin on the adherence of rat PMNs to the endothelia activated by burn sera.
  
  
  METHODS
  Pulmonary microvascular endothelial cells (PMEC) of rat were cultured by tissue block method. Rat PMNs were harvested by continuous perfusion. The samples were collected for the detection of the adhering rate of PMEC to the PMNs after being cultured with L-selectin monoclonal antibody (mAb), L-selectin ligand and sulfatide, respectively.
  
  
  RESULTS
  The adhering rate of PMNs to PMEC was obviously lowered by the adding of L-selectin mAb and sulfatide.
  
  
  CONCLUSIONS
  The adherence of PMNs to activated PMEC could be mediated by L-selectin.
authors.affiliation: Department of Surgery, The Eighty-fifth Hospited of PLA, 200052, P. R. China.
authors.forename: J
authors.initials: J
authors.lastname: Ni
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002056:Burns / Q000097:blood*; D002448:Cell Adhesion / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D019041:L-Selectin / Q000276:immunology / Q000494:pharmacology*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013433:Sulfoglycosphingolipids / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D007106:Immune Sera; D013433:Sulfoglycosphingolipids; D019041:L-Selectin