Interface for coupling nonaqueous wide-bore capillary electrophoresis with mass spectrometry.
A liquid-junction-type interface where a thin spraying capillary is inserted inside the separation capillary was constructed for coupling nonaqueous wide-bore capillary electrophoresis (CE) to mass spectrometry (MS). The robust structure of the interface provided fairly easy capillary handling. The study was carried out with uncoated CE capillaries of 200 and 320 microm inner diameter (ID). 1-Propanol-acetonitrile (80:20 v/v) with acetate electrolyte provided a low conducting medium for CE and good spraying conditions for electrospray ionization (ESI) without sheath-flow and drying gas. Methamphetamine, alprenolol, and levorphanol served as model compounds. Approximate detection limits with the 200 microm ID capillary were 35-265 ng/mL.
Varjo
Sami J O
SJ
Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, Finland.
Jussila
Matti
M
Palonen
Sami
S
Riekkola
Marja-Liisa
ML
D000433:1-Propanol; D000085:Acetates; D000097:Acetonitriles; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D000085:Acetates; D000097:Acetonitriles; D004573:Electrolytes; D000433:1-Propanol