title: Nitric oxide in ischemic and reperfused human muscle.
abstract: BACKGROUND
  Biochemical events explaining the pathology of ischemia-reperfusion in the muscle are still debated. Nitric oxide (NO) has been postulated to be implicated in these phenomena, but the short half-life of this compound makes it difficult to measure.
  
  
  METHODS
  In this paper, we used an amperometric solid-sate sensor to measure NO concentrations in frozen human muscles before, during and after a period of ischemia. We also measured cytochrome oxidase activity and malondialdehyde (MDA).
  
  
  RESULTS
  NO increased during ischemia but it soon returned to normal values upon reperfusion. On the other hand, cytochrome oxidase that also decreased in ischemic muscle did not increase during the reperfusion and malondialdehyde only increased during reperfusion, indicating the occurrence of peroxidative reactions in this situation.
  
  
  CONCLUSIONS
  NO is implicated in the ischemia/reperfusion pathology, but it is difficult to relate whether this is connected to cytochrome oxidase activity and malondialdehyde formation, also modified in this ischemia-reperfusion model.
authors.affiliation: Istituto di Anestesia e Rianimazione, Università di Cagliari, Italy.
authors.forename: Gian Giacomo
authors.initials: GG
authors.lastname: Corbucci
authors.forename: Biagio
authors.initials: B
authors.lastname: Lettieri
authors.forename: Valter
authors.initials: V
authors.lastname: Damonti
authors.forename: Roberto
authors.initials: R
authors.lastname: Palombari
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Arienti
authors.forename: Carlo Alberto
authors.initials: CA
authors.lastname: Palmerini
mesh_terms: D000328:Adult; D004579:Electron Transport / Q000502:physiology; D003576:Electron Transport Complex IV / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007511:Ischemia / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000378:metabolism; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D012039:Regional Blood Flow / Q000502:physiology; D015424:Reperfusion; D014111:Tourniquets
chemical_list: D007202:Indicators and Reagents; D009124:Muscle Proteins; D009569:Nitric Oxide; D008315:Malondialdehyde; D003576:Electron Transport Complex IV