title: Cloning and characterization of ARHGAP12, a novel human rhoGAP gene.
abstract: Rho GTPase activating proteins (GAPs) stimulate the intrinsic GTP hydrolysis activity of Rho family proteins. Here we report the cloning of two splice variants of a novel gene named ARHGAP12 (access number), which has an ORF of 2541bp. Profilescan search result showed that its putative protein contains five domains: rhoGAP, SH3, PH and two WW domains. ARHGAP12 is located in chromosome 10pter-cen and consists of 20 exons according to the Blastn result against high throughput genomic sequences (htgs). Reverse transcription PCR and Northern blot indicates that it ubiquitously expresses in human tissues as well as tumor cell lines, suggesting its basic roles in cells.
authors.affiliation: School of Life Science, State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: ChaoQun
authors.initials: C
authors.lastname: Wu
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: ZongXiang
authors.initials: Z
authors.lastname: Zhou
authors.forename: Kang
authors.initials: K
authors.lastname: Ying
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: YuMin
authors.initials: Y
authors.lastname: Mao
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D019295:Computational Biology; D005091:Exons; D020690:GTPase-Activating Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D018909:src Homology Domains
chemical_list: C454999:ARHGAP12 protein, human; D020690:GTPase-Activating Proteins; C085379:rho GTPase-activating protein