Telomerase regulation and telomere dynamics in germinal centers.
Telomere length maintenance, usually executed by telomerase, is a prerequisite for an extended or infinite division potential. Nevertheless most telomerase positive normal cells exhibit telomere shortening. This study details the telomerase expression and telomere dynamics in purified tonsil B cell subsets during the germinal center (GC) reaction. Significant telomere lengthening was observed as naive B cells matured to centroblasts and when centroblasts matured further to centrocytes, resulting in an increase in telomere length of about 4 kbp determined by Southern blotting. Immunopurified cell populations were also studied by fluorescence in situ hybridization and flow cytometry (flow-FISH) confirming that the GC B cells exhibited lengthened telomeres. These data were further verified in unpurified tonsil cells by combining flow-FISH and immunophenotyping using selected surface markers. Centroblasts expressed high levels of telomerase activity, which was increased in centrocytes, whereas resting naive, activated naive and memory B cells were telomerase activity negative. Expression levels of the catalytic subunit (hTERT) RNA paralleled the telomerase activity levels. The unique telomere elongation in GC B cells permits extensive proliferation during the GC reaction and provides the memory cells with a substantial increase in division potential. Understanding the telomere biology of GC cells is important in defining requirements for telomere elongation in vivo, with implications for the normal immune system as well as for lymphomas, and could provide insights into how the division potential of cells can be manipulated in vitro.
Norrback
K F
KF
Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.
Hultdin
M
M
Dahlenborg
K
K
Osterman
P
P
Carlsson
R
R
Roos
G
G
D015703:Antigens, CD; D016175:B-Lymphocyte Subsets / Q000201:enzymology* / Q000648:ultrastructure; D005434:Flow Cytometry; D018858:Germinal Center / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D014066:Palatine Tonsil / Q000201:enzymology / Q000648:ultrastructure; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000648:ultrastructure*
D015703:Antigens, CD; D019098:Telomerase