[The immune response to artificial proteins containing biologically active fragments of human IFN-alpha2 and insulin].
A study was made of the humoral immune response of BALB/c mice to various doses of artificial proteins that contained biologically active fragments of human interferon alpha 2 (IFN-alpha 2) and insulin. The insulin fragment had no effect on the response to any protein construct. The IFN-alpha 2 fragment increased the titer of antibodies against the construct. Mapping of continuous B epitopes with immune sera revealed several antigenic determinants, the C end of the IFN-alpha 2 fragment with the adjacent de novo protein region being immunodominant. More effective binding of serum antibodies with the constructs containing the IFN-alpha 2 fragment was attributed to antibody interaction with the fragment and to a better recognition of the entire protein construct by the immune system.
Bocharova
O V
OV
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia. obon@nmr.ru
Moshkovskiĭ
S A
SA
Chertkova
R V
RV
Kolesanova
E F
EF
Dolgikh
D A
DA
Kirpichnikov
M P
MP
D000818:Animals; D000939:Epitopes; D006801:Humans; D007328:Insulin / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
D000939:Epitopes; D007328:Insulin; D016898:Interferon-alpha; D010446:Peptide Fragments; D011506:Proteins; D011994:Recombinant Proteins; C074146:albebetin