title: [Nucleic acid quantifying assay of hepatitis B virus].
abstract: OBJECTIVE
  To explore the relation between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state.
  
  
  METHODS
  Thirty-nine patients suffering from chronic hepatitis B with positive HBsAg were performed liver puncture for histopathological examination. Fluorescence ration PCR analysis was used for HBV DNA quantifying assessment. Hepatitis B virus markers were detected by ELISA.
  
  
  RESULTS
  Serum HBV DNA content was related to hepatitis B virus markers. There was no correlation between HBV DNA level and the Scheuer grade in patients with positive HBsAg, and no obvious correlation between serum glutamic pyruvic transaminase level and HBV DNA content.
  
  
  CONCLUSIONS
  There is a significant correlation between HBeAg and HBV DNA, but not between liver inflammation stage and serum HBV DNA content.
authors.affiliation: Department of Infectious Diseases, the Fourth Hospital of Nanning, Guangxi 530023, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianghua
authors.initials: J
authors.lastname: Zhu
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D002648:Child; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005453:Fluorescence; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006513:Hepatitis B e Antigens / Q000097:blood*; D006515:Hepatitis B virus / Q000737:chemistry* / Q000235:genetics; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D000410:Alanine Transaminase