title: Crystallization and preliminary X-ray diffraction analysis of chloroplastic ascorbate peroxidase of tobacco plants.
abstract: The stromal ascorbate peroxidase of tobacco plants was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 4000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 76.8, c = 98.8 A. The calculated V(M) value based on a monomer in the asymmetric unit is 2.2 A(3)Da(-1). A data set was successfully collected to 1.6 A resolution from a frozen crystal using synchrotron radiation of wavelength 1.0 A at KEK-PF, Japan.
authors.affiliation: Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan. keiwada@biochem.osakafu-u.ac.jp
authors.forename: Kei
authors.initials: K
authors.lastname: Wada
authors.forename: Toshiji
authors.initials: T
authors.lastname: Tada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yabuta
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Toru
authors.initials: T
authors.lastname: Takeda
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shigeoka
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D060387:Ascorbate Peroxidases; D002736:Chloroplasts / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010544:Peroxidases / Q000737:chemistry*; D011487:Protein Conformation; D014026:Nicotiana / Q000737:chemistry / Q000201:enzymology*
chemical_list: D010544:Peroxidases; D060387:Ascorbate Peroxidases