[Effect of carbon disulfide on pyrogallol- luminol auto-oxidation chemiluminescence system and superoxide anion].
OBJECTIVE
To study the effect of carbon disulfide (CS(2)) on pyrogallol-luminol auto-oxidation chemiluminescence (PA-L) system and production of superoxide anion.


METHODS
Luminescence dynamic curve of CS(2) and inhibition effect of superoxide dismutase (SOD) on intensity of chemiluminescence induced by CS(2) were studied with PA-L chemiluminescence system.


RESULTS
Different concentrations of CS(2) (10, 40, 80 mg/ml) could enhance the emission intensity of chemiluminescence of PA-L system and delayed its peak time, with a significant dose-effect relationship, as compared with that of solvent ethanol. Peak of chemiluminescence induced by 80 mg/ml of CS(2) was higher than its background value without solvent ethanol. Chemiluminescence induced by CS(2) could be inhibited by SOD.


CONCLUSIONS
CS(2) can generate superoxide anion in PA-L system and delay the peak time of chemiluminescence emission dynamic curve.
Jian
L
L
Research Laboratory of Occupational Poisoning, School of Public Health, Zhejiang University, Hangzhou 310031, China.
Chen
Q
Q
D000838:Anions / Q000737:chemistry; D002246:Carbon Disulfide / Q000737:chemistry* / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D011748:Pyrogallol / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
D000838:Anions; D011748:Pyrogallol; D013481:Superoxides; D000431:Ethanol; D008165:Luminol; D013482:Superoxide Dismutase; D002246:Carbon Disulfide