title: A high fat meal activates blood coagulation factor VII in rats.
abstract: In humans, high fat meals cause postprandial activation of blood coagulation factor VII (FVII), but human studies have not provided definite evidence for a prothrombotic effect of dietary FVII activation. An animal model would be an attractive way to pursue this question and therefore we tested the LEW/Mol rat. We gavaged 3 mL of a fat emulsion (n = 42) or 3 mL isotonic glucose (n = 42). Blood was sampled by heart puncture 2, 4 and 6 h (n = 14/group at each time) after the fat/glucose load. Furthermore, blood was sampled from 16 untreated rats to determine the baseline levels. Triglyceride concentrations, activated FVII (FVIIa), FVII coagulant activity (FVIIc), FVII amidolytic activity (FVIIam) and thrombin-antithrombin complexes (TAT) were determined. After fat administration, triglycerides were significantly elevated at 2 h (1.29 mmol/L) and 4 h (1.37 mmol/L) compared with baseline (0.78 mmol/L), and FVIIa was significantly raised at 4 h (54 U/L) and 6 h (58 U/L) compared with baseline (29 U/L). No postprandial changes in FVIIc, FVIIam and TAT were observed. Glucose administration did not affect any variable. We conclude that the LEW/Mol rat is a promising model for use in future studies of thrombotic effects of dietary FVII activation.
authors.affiliation: Department for Thrombosis Research, Ribe County Hospital, University of Southern Denmark, Esbjerg, Denmark. aako@ribeamt.dk
authors.forename: Aage K
authors.initials: AK
authors.lastname: Olsen
authors.forename: Else M
authors.initials: EM
authors.lastname: Bladbjerg
authors.forename: Axel K
authors.initials: AK
authors.lastname: Hansen
authors.forename: Peter
authors.initials: P
authors.lastname: Marckmann
mesh_terms: D000577:Amides / Q000378:metabolism; D000818:Animals; D000990:Antithrombin III / Q000032:analysis; D001777:Blood Coagulation; D004041:Dietary Fats / Q000008:administration & dosage*; D004655:Emulsions; D005167:Factor VII / Q000378:metabolism*; D015942:Factor VIIa / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000008:administration & dosage; D010447:Peptide Hydrolases / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D000577:Amides; D004041:Dietary Fats; D004655:Emulsions; D014280:Triglycerides; C046193:antithrombin III-protease complex; D000990:Antithrombin III; D005167:Factor VII; D010447:Peptide Hydrolases; D015942:Factor VIIa; D005947:Glucose