title: Preliminary research on myosin light chain kinase in rabbit liver.
abstract: OBJECTIVE
  To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver.
  
  
  METHODS
  The expression of MLCK was detected by reverse transcription-polymerase chain reaction(RT-PCR); the MLCK was obtained from rabbit liver, and its activity was analyzed by gamma-(32)P incorporation technique to detect the phosphorylation of myosin light chain.
  
  
  RESULTS
  MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin-dependent. When the concentration was 0.65 mg x L(-1), the activity was at the highest level.
  
  
  CONCLUSIONS
  MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Science and Technology of China, Hefei 230027, Anhui Province, China.
authors.forename: B
authors.initials: B
authors.lastname: Ren
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhu
authors.forename: Z F
authors.initials: ZF
authors.lastname: Luo
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D022781:Hepatocytes / Q000201:enzymology; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D018994:Myosin Light Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits
chemical_list: D018994:Myosin Light Chains; D009219:Myosin-Light-Chain Kinase