Mouse eye gene microarrays for investigating ocular development and disease.
Microarray technology can facilitate simultaneous expression analysis of thousands of genes and assist in delineating cellular pathways involved in development or disease pathogenesis. Since public databases and commercial cDNA microarrays have an under-representation of eye-expressed genes, we generated over 3000 expressed sequence tags from three unamplified mouse eye/retina cDNA libraries. These eye-expressed genes were used to produce cDNA microarrays. Methodology for printing of slides, hybridization, scanning and data analysis has been optimized. The I-gene microarrays will be useful for establishing expression profiles of the mouse eye/retina and provide a resource for defining molecular pathways involved in development, aging and disease.
Farjo
Rafal
R
Department of Ophthalmology, W.K. Kellogg Eye Center, University of Michigan, 1000 Wall Street, Ann Arbor, MI 48105, USA.
Yu
Jindan
J
Othman
Mohammad I
MI
Yoshida
Shigeo
S
Sheth
Sujata
S
Glaser
Tom
T
Baehr
Wolfgang
W
Swaroop
Anand
A
D000818:Animals; D020224:Expressed Sequence Tags; D005136:Eye Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D009024:Morphogenesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D012160:Retina / Q000196:embryology*; D012162:Retinal Degeneration / Q000196:embryology*
D005136:Eye Proteins