title: Characterization of transthyretin variants in familial transthyretin amyloidosis by mass spectrometric peptide mapping and DNA sequence analysis.
abstract: Transthyretin (TTR) is a 127-amino acid residue transport protein. In plasma, TTR exists as a tetramer and binds the hormone thyroxine and the retinol-binding protein-vitamin A complex. Amino acid substitutions in TTR are hypothesized to destabilize the tetramer and cause the protein to form intermediates that self-associate into amyloid fibrils. Familial transthyretin amyloidosis (ATTR) is associated with extracellular deposition of wild-type TTR, its variants or fragments as amyloid fibrils in various tissues and organs. A definitive diagnosis of ATTR depends on the detection and identification of TTR variants. Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS), in combination with trypsin digestion, have been shown to be powerful tools in characterizing TTR variants. Typically, TTR or its tryptic digest is analyzed by MALDI-TOF MS, liquid chromatography ESI MS, or both. Analysis of tryptic digests by MALDI-TOF MS does not provide enough sequence coverage in TTR to identify all possible modifications. To improve sequence coverage, aliquots of immunoprecipitated TTR samples were digested with trypsin, lysyl endopeptidase Lys-C, or endoproteinase Asp-N. Identification of the peptides from each digest by MALDI-TOF MS provided preliminary information about the sites and mass shifts due to amino acid substitutions from genetic mutations and to posttranslational modifications. The location and identity of the modifications in the variant proteins were then confirmed by tandem mass spectrometry, accurate mass measurements, and direct DNA sequence analysis. Using these methodologies, we achieved 100% sequence coverage. The detection of two nonpathologic variants (Thr119Met and Gly6Ser) and four pathologic variants (Phe64Leu, Asp38Ala, Phe44Ser, and previously unreported Trp41Leu) are described as illustrations of this approach.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA.
authors.forename: Amareth
authors.initials: A
authors.lastname: Lim
authors.forename: Tatiana
authors.initials: T
authors.lastname: Prokaeva
authors.forename: Mark E
authors.initials: ME
authors.lastname: McComb
authors.forename: Peter B
authors.initials: PB
authors.lastname: O'Connor
authors.forename: Roger
authors.initials: R
authors.lastname: Théberge
authors.forename: Lawreen H
authors.initials: LH
authors.lastname: Connors
authors.forename: Martha
authors.initials: M
authors.lastname: Skinner
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010449:Peptide Mapping / Q000379:methods*; D011228:Prealbumin / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011228:Prealbumin; D004247:DNA