[The postburn change in splenic T lymphocyte transmembrane signal transduction and its relationship with the secretion of IL-2 and IL-10 in severely scalded mice].
OBJECTIVE
To explore the postburn functional change in splenic T lymphocytes and the secretory rules of IL-2 and IL-10 in severely scalded mice, and to look for the explanation of the change by means of T lymphocyte transmembrane signal transdunction study.


METHODS
The changes in the antigen receptors on T cells (TCRalpha/beta), assisting stimulating molecule (CD28) and the activities of GTPase, protein tyrosine kinase (PTK) and protein kinase C (PKC) that participated in the transmembrane signal transduction were detected, so as to observe the proliferation and transformation function of T cells at different post-burn periods and the secretion of IL-2 and IL-10. Furthermore, the changes in every signal transduction molecules were analyzed with the consideration of their relationship with the change in T cell function activities.


RESULTS
The postburn positive rates of the expressions of TCRalpha/beta and CD28 on T cell membrane decreased. The postburn GTPase activity and membrane PTK activity were all suppressed but recovered at 168 postburn hours. Membrane PKC activity exhibited a dual-phase change (increase following decrease), which was closely related to IL-10 level.


CONCLUSIONS
The postburn changes in TCRalpha/beta, CD28 on T cell membrane molecules and transmembrane signal transduction enzymes were important factors contributing to the decrease in IL-2 secretion, suppressed T cell function and the dual-directional changes in IL-10 secretion.
Zheng
J
J
Institute of Burn Research, Southwestern Hospital, Third Military Medical University, Chongqing 400038, P. R. China.
Wu
J
J
Peng
D
D
D000818:Animals; D002056:Burns / Q000276:immunology*; D018106:CD28 Antigens / Q000032:analysis; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*
D018106:CD28 Antigens; D007376:Interleukin-2; D016753:Interleukin-10