[Experimental observation of foreign gene transfer with lipofectin to the primary human lens epithelial cells].
OBJECTIVE
To determine whether an exogenous gene carried by lipofectin can be introduced into the primary human lens epithelial cells.


METHODS
Plasmid DNA with beta-galactosidase gene carried by lipofectin was applied to primary cultured human lens epithelial cells. Gene expression was detected by enzymatic color reaction using X-gal as a substrate in the 2 and 6 days of expression after 12, 24, 36 hours of transfection respectively. The gene transfer positive rate of the lens epithelial cells was counted.


RESULTS
The foreign gene could be transferred into primary human lens epithelial cells by lipofectin. In the expression of 2 days, transfer positive rate was up to 48% after 24 hours of transfection.


CONCLUSIONS
Efficient and stable transfer of the functional gene can be achieved by lipofectin into the lens epithelial cells. Lipofectin is an available and a promising vehicle for delivering aim gene and studying on the mechanism of physiology and pathology of lens epithelial cells.
Liu
D
D
Department of Ophthalmology, First Hospital, Beijing Medical University, Beijing 100034.
Wu
J
J
D004847:Epithelial Cells / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000008:administration & dosage*
D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine