Generic method for on-line extraction of drug substances in the presence of biological matrices using turbulent flow chromatography.
The use of liquid chromatography/mass spectrometry (LC/MS) to quantify drugs in biological matrices has been well established over the last decade. Extremely fast LC/MS methods are commonplace in the pharmaceutical industry for high-throughput Absorption, Distribution, Metabolism and Excretion (ADME) screening. However, to truly take full advantage of high-throughput ADME screening, a generic method is needed that eliminates the need to develop a new method for each new compound being screened. New developments in the stationary phase of turbulent flow columns has allowed us to develop an on-line biological sample cleanup method that is suitable for over 99% of the compounds in the Cephalon database.
Herman
J L
JL
Cephalon, Inc., 145 Brandywine Parkway, West Chester, PA 19380-4245, USA. jherman@cephalon.com
D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D009862:Online Systems; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents
D004364:Pharmaceutical Preparations; D012997:Solvents