title: Inhibitory effect of tumor cell proliferation and induction of G2/M cell cycle arrest by panaxytriol.
abstract: Panaxytriol, a polyacetylenic compound, isolated from red ginseng (Panax ginseng C.A. Meyer), was studied to determine its effects on the growth and cell cycle of tumor cell lines. The compound showed both significant cytotoxicity and inhibition of DNA syntheses in various tumor cells tested. For P388D1, a mouse lymphoma cell line, IC50 values for cytotoxicity and inhibition of DNA synthesis were 3.1 and 0.7 microg/ml, respectively. The cytotoxic effect of panaxytriol was both time- and dose-dependent. It also induced the cell cycle arrest of P388D1 at the G2/M phase, which was measured through flow cytometry. Particularly, the proportion of cells in the G2/M phase of the cell cycle increased from 9 % to 26 and 48 %, respectively, after 24 and 36 h exposure to panaxytriol at 5 microg/ml. There were corresponding decreases in the proportion of cells at the G0/G1 phase. The S phase also decreased during the 36-h treatment.
authors.affiliation: Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University, Suwon, Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Sun-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Jae Joon
authors.initials: JJ
authors.lastname: Wee
authors.forename: Young-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D016475:3T3 Cells; D000480:Alkynes; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D053281:Enediynes; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008518:Medicine, East Asian Traditional; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D015394:Molecular Structure; D017239:Paclitaxel / Q000494:pharmacology; D005894:Panax; D010936:Plant Extracts / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D020298:U937 Cells
chemical_list: D000480:Alkynes; D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D053281:Enediynes; D005233:Fatty Alcohols; D010936:Plant Extracts; D013778:Tetrazolium Salts; D013844:Thiazoles; C057682:panaxytriol; C022616:thiazolyl blue; D017239:Paclitaxel