Determination of ginsenosides from Panax ginseng using micellar electrokinetic chromatography.
A method for determination of ginsenosides in herbal medicinal products (HMPs) was developed using micellar electrokinetic chromatography (MEKC). Within 22 minutes 7 major ginsenosides were well separated. In order to demonstrate the accuracy, precision and robustness for the main target analyte the method was exemplarily validated for the determination of Rb1 according to ICH guidelines. Compared to chromatographic analysis, several benefits of capillary electrophoresis (CE) could be demonstrated such as high separation efficiency in an aqueous buffer without any organic solvent and shorter run time per assay.
Glöckl
Ingmar
I
Forschungsvereinigung der Arzneimittel-Hersteller e.V., Sinzig, Germany.
Veit
Markus
M
Blaschke
Gottfried
G
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D036145:Ginsenosides; D015394:Molecular Structure; D005894:Panax; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013057:Spectrum Analysis
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins