Comparison of molecular and conventional methods for typing of enteroviral isolates.
Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.
Manayani
Darly Joseph
DJ
Department of Clinical Virology, Christian Medical College and Hospital, Vellore-632004, India.
Shaji
R V
RV
Fletcher
G John
GJ
Cherian
Thomas
T
Murali
N
N
Sathish
N
N
Solomon
Tilak
T
Gnanamuthu
Chandran
C
Sridharan
Gopalan
G
D015373:Bacterial Typing Techniques / Q000379:methods*; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins; D004770:Enterovirus / Q000145:classification*; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D036022:Capsid Proteins