title: A cationic sub-micron emulsion (MF59/DOTAP) is an effective delivery system for DNA vaccines.
abstract: A novel cationic emulsion was developed to adsorb plasmid DNA and improve intracellular delivery of plasmid DNA upon immunization. The emulsion developed, had a highly uniform particle and charge distribution. Based on observations with cationic microparticles, the cationic emulsion was evaluated in vivo in mice and rabbits with a model HIV-1 pCMVp55 gag DNA. In both these species, the cationic emulsion engendered higher antibody responses than those obtained with naked DNA. The cationic emulsion also maintained the cellular responses seen with naked DNA at the same doses.
authors.affiliation: Chiron Corporation, Mail Stop 4.3, 4560 Horton Street, Emeryville, CA 94608, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Ott
authors.forename: Manmohan
authors.initials: M
authors.lastname: Singh
authors.forename: Jina
authors.initials: J
authors.lastname: Kazzaz
authors.forename: Maylene
authors.initials: M
authors.lastname: Briones
authors.forename: Elawati
authors.initials: E
authors.lastname: Soenawan
authors.forename: Mildred
authors.initials: M
authors.lastname: Ugozzoli
authors.forename: Derek T
authors.initials: DT
authors.lastname: O'Hagan
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D002412:Cations / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage*; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011136:Polysorbates / Q000008:administration & dosage*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage*; D011817:Rabbits; D013185:Squalene / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D002412:Cations; D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C089950:MF59 oil emulsion; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D019444:Vaccines, DNA; D013185:Squalene; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane