Meiotic chromosomes as templates for microdissection.
As templates for chromosome microdissection, meiotic cells offer several advantages over mitotic cells. The pairing of homologous chromosomes at the metaphase plate of the first meiotic division allows the simultaneous isolation of two copies of the same chromosome, and the sex chromosomes are easy to identify in male meiotic cells. We report on a method for making fluorescence in-situ hybridization (FISH) probes from dissected meiotic chromosomes.
Christian
Allen T
AT
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, CA 94551, USA. christian4@llnl.gov
Pattee
Melissa S
MS
Marchetti
Francesco
F
D000818:Animals; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D004210:Dissection / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008540:Meiosis; D051379:Mice; D008815:Mice, Inbred Strains; D013698:Templates, Genetic; D014960:X Chromosome; D014998:Y Chromosome