title: Autologous skin transplantation: comparison of minced skin to other techniques.
abstract: BACKGROUND
  Skin grafting may be necessary to close nonhealing skin wounds. This report describes a fast and minimally invasive method to produce minced skin suitable for transplantation to skin wounds. The technique was evaluated in an established porcine skin wound healing model and was compared to split-thickness skin grafts and suspensions of cultured and noncultured keratinocytes.
  
  
  METHODS
  The study included 90 wounds on 3 pigs. Fluid-treated full-thickness skin wounds were grafted with minced skin, split-thickness skin grafts, noncultured keratinocytes, or cultured keratinocytes. Controls received either fluid or dry treatment. The wound healing process was analyzed in histologies collected at Days 8 to 43 postwounding. Wound contraction was quantified by photoplanimetry.
  
  
  RESULTS
  Wounds transplanted with minced skin and keratinocyte suspension contained several colonies of keratinocytes in the newly formed granulation tissue. During the healing phase, the colonies progressed upward and reepithelialization was accelerated. Minced skin and split-thickness skin grafts reduced contraction as compared to keratinocyte suspensions and saline controls. Granulation tissue formation was also reduced in split-thickness skin-grafted wounds.
  
  
  CONCLUSIONS
  Minced skin grafting accelerates reepithelialization of fluid-treated skin wounds. The technique is faster and less expensive than split-thickness skin grafting and keratinocyte suspension transplantation. Minced skin grafting may have implications for the treatment of chronic wounds.
authors.affiliation: Laboratory of Tissue Repair and Gene Transfer, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: Tor
authors.initials: T
authors.lastname: Svensjö
authors.forename: Bohdan
authors.initials: B
authors.lastname: Pomahac
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.forename: Jaromir
authors.initials: J
authors.lastname: Slama
authors.forename: Nabil
authors.initials: N
authors.lastname: Wasif
authors.forename: Elof
authors.initials: E
authors.lastname: Eriksson
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004848:Epithelium / Q000293:injuries* / Q000473:pathology; D005260:Female; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation; D019060:Minimally Invasive Surgical Procedures / Q000295:instrumentation / Q000379:methods; D016038:Skin Transplantation / Q000379:methods*; D013552:Swine; D014945:Wound Healing