title: Oligomerization of the Bacillus subtilis division protein DivIVA.
abstract: DivIVA appears to be a mediator of inhibition by MinCD of division at the cell poles in Bacillus subtilis. Gel permeation and ultracentrifugation techniques were used to show self-association of DivIVA into a form consisting of 10-12 monomers in vitro. Western blot analysis of non-denaturating polyacrylamide gels confirms the presence of similar oligomers in B. subtilis cell lysates. These oligomers persist in a B. subtilis strain containing the divIVA1 mutation, in which proper vegetative septum positioning is abolished. In contrast, the divIVA2 mutation, which has a similar biological impact, appears to produce a protein with different oligomerization properties. The results of the present study suggest that oligomerization of DivIVA is important, but not sufficient for its function in the cell division process.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
authors.forename: Katarı Na
authors.initials: KN
authors.lastname: Muchová
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
authors.forename: Eva
authors.initials: E
authors.lastname: Kutejová
authors.affiliation: Department of Biology, University of York, Heslington YO10 5YW, UK3.|Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
authors.forename: David J
authors.initials: DJ
authors.lastname: Scott
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
authors.forename: James A
authors.initials: JA
authors.lastname: Brannigan
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Lewis
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK2.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Wilkinson
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia1.
authors.forename: Imrich
authors.initials: I
authors.lastname: Barák
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012394:Rosaniline Dyes; D014461:Ultracentrifugation
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; C104636:DivIVA protein, bacteria; D012394:Rosaniline Dyes; C048139:Coomassie blue