title: Effect of dietary protein quantity and quality on the brain protein synthesis rate in ovariectomized female rats.
abstract: The purpose of this study was to determine whether the quantity and quality of dietary protein affected the rate of brain protein synthesis in ovariectomized female rats. Two experiments were conducted on the ovariectomized female rats (12 weeks old) given diets containing 20%, 5%, or 0% casein (experiment 1) and 20% casein, 20% soy protein, 20% gluten, or 20% gelatin (experiment 2) for 10 d, respectively. The fractional rates of protein synthesis in the brain declined with a decrease of the quantity and quality of dietary protein. In the brain, the RNA activity [g of protein synthesized/((g of RNA) d)] was significantly correlated with the fractional rate of protein synthesis. The RNA concentration (mg of RNA/g of protein) was not related to the fractional rate of protein synthesis in any organ. The results suggest that the rate of protein synthesis in the brain declines with the decrease of the quantity and quality of dietary protein in ovariectomized female rats, and that RNA activity is at least partly related to the fractional rate of brain protein synthesis.
authors.affiliation: Department of Home Economics, Aichi University of Education, Kariya, Aichi 448-8542, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Sunok
authors.initials: S
authors.lastname: Lyou
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Hayase
authors.forename: Satoshi
authors.initials: S
authors.lastname: Okuyama
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Yokogoshi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002364:Caseins / Q000008:administration & dosage; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D005780:Gelatin / Q000008:administration & dosage; D005983:Glutens / Q000008:administration & dosage; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010052:Ovariectomy; D012313:RNA / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D030262:Soybean Proteins / Q000008:administration & dosage
chemical_list: D002364:Caseins; D004044:Dietary Proteins; D009419:Nerve Tissue Proteins; D030262:Soybean Proteins; D012313:RNA; D005983:Glutens; D005780:Gelatin