Distribution of hepatitis C virus RNA in whole blood of patients with HCV infection and HBV-HCV coinfection.
BACKGROUND
Although HCV and HBV are essentially hepatoptropic, several lines of evidence suggest that these viruses can infect other cells, also PBMC in most patients with chronic HCV.


METHODS
The presence of HBV DNA and HCV-RNA was determined by a polymerase chain reaction (multiplex PCR and RT-PCR - nested PCR) in a group of patients with chronic liver disease. HCV-RNA was investigated in serum, plasma and peripheral blood mononuclear cells (PBMC) while HBV-DNA only in serum or plasma.


RESULTS
Among 374 patients tested, HCV-RNA was detected in the venous blood of 208 patients; HCV RNA alone was detected in 154 patients and 54 patients were co-infected by HCV and HBV. HBV-DNA was found in 128 of 374 patients, while infection by HBV only was found in 74 patients. It was also shown that in the presence of HBV the replication ability of HCV is lower (p=0.085, Goodman-Kruskal Gamma = 0.561 and YuleQ = 0.5610).


CONCLUSIONS
Since coexistence of HBV and HCV is not a rare case, diagnostics of hepatitis cannot be limited to detection of one type of the virus only. Misinterpretation of the virus type that caused the infection may lead to serious complications, especially in those cases when interferone is used for treatment.
Mazurek
Urszula
U
Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Silesian Medical University, ul. Narcyzów 1, 41-200 Sosnowiec, Poland.
Wilczok
Tadeusz
T
Mazur
Włodzimierz
W
Bednarek
Ilona
I
Strzałka
Barbara
B
Jurzak
Magdalena
M
Laskowska-Lepiarczyk
Ewa
E
Gonciarz
Zbigniew
Z
D001483:Base Sequence; D017931:DNA Primers; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D006509:Hepatitis B / Q000097:blood* / Q000150:complications; D006526:Hepatitis C / Q000097:blood* / Q000150:complications; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D012680:Sensitivity and Specificity; D014779:Virus Replication
D017931:DNA Primers; D012367:RNA, Viral