[A study of the correlation between p16 protein expression and DNA quantitative analysis in human gastric carcinoma cells].
OBJECTIVE
To investigate p16 protein expression and its correlation with DNA quantity in gastric carcinoma (GC) cells.


METHODS
p16 protein expression was quantitatively determined by flow cytometry (FCM) in 41 cases of GC and 10 cases of normal gastric tissues. DNA quantitative analysis of 41 cases of GC was performed by image cytometry (ICM).


RESULTS
p16 protein expression in GC was significantly lower than that in normal gastric tissues (respective FI: 0.81 plus minus 0.09 and 1.00 plus minus 0.11, P < 0.01). The expression of p16 protein did not show correlation with such factors as sex and age in GC, but it was significantly stronger in well differentiated GC than that in poorly differentiated GC (P < 0.05). The mean of DNA relative ploid (U value) in GC was from 1.14 to 2.98. U value becomes higher with the increase in grading (P < 0.01). There was significant negative linear correlation between p16 protein expression and U value in GC (P < 0.01).


CONCLUSIONS
Abnormal expression of p16 protein is closely correlated to the occurrence, development and malignancy degree of GC. The level of p16 protein expression implies important clinical significance for GC treatment and may serve as an indicator to predict the prognosis of patients.
Gao
M
M
Department of Pathology, Shanxi Medical University, Taiyuan 030001, China. gaomeixia@sohu.com
Zhang
N
N
Ji
C
C
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D018279:Carcinoma, Signet Ring Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm