Immunoassay method for quantitative determination of nisin in solution and on polymeric films.
OBJECTIVE
To develop a non-competitive direct immunoassay method for quantifying nisin in solution or adsorbed to surfaces.


RESULTS
The developed method differs from traditional ELISA in pre-forming a complex of the nisin antibodies and the secondary antibody conjugate before addition to the nisin-coated solid phase. The modifications may help decrease interference from the nisin degradation molecules and reduce non-specific adsorption of secondary antibody conjugate. The new method has a detection limit of 65 ng nisin and correlates well (r=0.982) with the agar diffusion bioassay.


CONCLUSIONS
A new sensitivity method was developed to determine the amounts of nisin adsorbed to a polymeric surface.


CONCLUSIONS
The new technique can provide a reproducible and accurate method to quantitate nisin molecules in solutions and those bound on polymeric surfaces, and it is free of the limitations of the traditional agar diffusion bioassay.
Leung
P P
PP
Department of Food Science and Technology, The Ohio State University, Columbus, 43210, USA.
Khadre
M
M
Shellhammer
T H
TH
Yousef
A E
AE
D001681:Biological Assay / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D007118:Immunoassay / Q000379:methods*; D009561:Nisin / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D012996:Solutions
D011108:Polymers; D012996:Solutions; D009561:Nisin