Invertase and urease activities in the carotenogenic yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma).
Invertase and urease are enzyme entities highly associated with the cells of the astaxanthin-producer yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) during any stage of its cell growth cycle. In this study cellobiose was a more efficient carbon source than sucrose or its hexose counterparts for invertase expression. Extensive ultrasonication or abrasion with glass pearls were required in order to promote enzyme release. In contrast to the yeast whose growth declines above 27 degrees C, the released enzymes displayed a higher optimum temperature range when assayed in vitro. Isoforms from both enzymes could be resolved either by FPLC on DEAE-Sepharose or by an affinity approach on immobilized Concanavalin. The zymogram for invertase showed a pI somewhat less acidic than that of the similar enzyme from S. cerevisiae.
Persike
Daniele S
DS
UNIANDRADE and UTP-Tuiuti University of Paraná, Brazil.
Bonfim
Tânia M B
TM
Santos
Maria H R
MH
Lyng
Sabrina M O
SM
Chiarello
Marileusa D
MD
Fontana
José D
JD
D002455:Cell Division / Q000502:physiology; D002475:Cellobiose / Q000378:metabolism*; D002846:Chromatography, Affinity; D004586:Electrophoresis; D004790:Enzyme Induction; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013010:Sonication; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism*; D013696:Temperature; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015003:Yeasts / Q000201:enzymology* / Q000378:metabolism; D043324:beta-Fructofuranosidase
D004791:Enzyme Inhibitors; D002475:Cellobiose; D013395:Sucrose; D014508:Urea; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D014510:Urease