Differential esterase expression in developmental mutants of Aspergillus nidulans.
Esterase isozymes were used to detect substrate-preference polymorphism in five strains of Aspergillus nidulans, and to show differential gene expression in developmental mutants in response to 5-azacytidine treatment. The medusa mutants B116, SM23, and M25 were selected in the presence of 5-azacytidine (5AC); also the G839 bristle mutant obtained in the absence of 5AC as well as the UT196 master strain and the normal segregant SM24 were used for the esterase studies. The esterase isozyme patterns of the A. nidulans strains observed with 4-methylumbelliferyl esters and alpha- and beta-naphthyl esters indicated a total of 18 isoesterases. Substrate preference for either 4-methylumbelliferyl esters and alpha- or beta-naphthyl esters was observed. Similarity between the different A. nidulans genotypes was 84.4-100%. The genomic similarity of the B116, SM23, and M25 mutant strains (100%) supports previous observations that specific DNA sequences might be targets for 5AC action in this filamentous fungus, and the differential expression of the Est-4 isozyme in the medusa developmental mutant and the Est-2 isozyme specifically detected in the bristle mutant G839 seems to indicate esterase isozymes as possible markers of biochemical differences among different developmental mutants of A. nidulans.
Machado
M F
MF
Department of Cell Biology and Genetics, State University of Maringa, Parana, Brazil.
de Castro-Prado
M A
MA
D001233:Aspergillus nidulans / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D001374:Azacitidine / Q000494:pharmacology; D004950:Esterases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D011110:Polymorphism, Genetic; D013379:Substrate Specificity
D007527:Isoenzymes; D004950:Esterases; D001374:Azacitidine