Chemistry and biocompatibility of alginate-PLL capsules for immunoprotection of mammalian cells.
Transplantation of encapsulated living cells is a promising approach for the treatment of a wide variety of diseases. Large-scale application of the technique, however, is hampered by insufficient biocompatibility of the capsules. In order to get means to study factors influencing the biocompatibility of capsule for encapsulation of living cells, we have correlated the chemical composition of the surface of commonly applied alginate-PLL capsules with the biological response in rats. Capsules prepared of alginates with an intermediate guluronic (G) acid content proved to be biocompatible, whereas capsules prepared of high-G alginates were overgrown by inflammatory cells. We applied X-ray photoelectron spectroscopy to correlate the biological responses with the chemical compositions of the capsule surfaces. High-G alginate capsules proved to have a higher PLL content but less surface binding sites for PLL than low-G alginates. This study, shows for the first time that biological responses against capsules can be successfully correlated to its chemical characteristics.
de Vos
Paul
P
Department of Pathology and Laboratory Medicine, Section of Medical Biology, University of Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands. P.DE.VOS@MED.RUG.NL
Hoogmoed
Chris G
CG
Busscher
Henk J
HJ
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D017690:Cell Transplantation / Q000379:methods*; D004339:Drug Compounding; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D008422:Materials Testing; D011107:Polylysine / Q000737:chemistry*; D051381:Rats; D013052:Spectrometry, X-Ray Emission
D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; C007896:guluronic acid; D011107:Polylysine; C008324:mannuronic acid