[A study of hemopoiesis on a sublayer of peritoneal cells in the presence of hemopoietic cytokines].
We studied the effects of erythropoietin and thrombopoietin on the clonogenic capacity and direction of differentiation of the hemopoietic cells that form colonies on acetate cellulose membrane in the peritoneal cavity of mice. An increased level of erythropoietin in the blood of recipient mice after blood letting led to the appearance of erythroid colonies upon transplantation of syngeneic hemopoietic cells but did not affect the differentiation of transplanted xenogeneic (guinea pig) hemopoietic cells. Erythropoietin transported top the stromal sublayer by a polymeric carrier also induced erythroid differentiation, while thrombopoietin transported in a similar way somewhat enhanced megakaryocytopoiesis.
Paiushina
O V
OV
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.
Michurina
T V
TV
Satdykova
G P
GP
Nikonova
T M
TM
Khrushchov
N G
NG
D000818:Animals; D016026:Bone Marrow Transplantation; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004921:Erythropoietin / Q000097:blood / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D006410:Hematopoiesis; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology*; D011145:Polyvinyls; D017154:Stromal Cells / Q000187:drug effects; D013926:Thrombopoietin / Q000097:blood / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D019737:Transplants
D011145:Polyvinyls; D004921:Erythropoietin; C016438:ethylenevinylacetate copolymer; D013926:Thrombopoietin