Interferon beta-1b modulates MCP-1 expression and production in relapsing-remitting multiple sclerosis.
Monocyte chemoattractant protein-1 (MCP-1) seems to be involved in the pathogenesis of multiple sclerosis (MS). We found that in unstimulated (PHA(-)) and PHA-stimulated (PHA(+)) peripheral blood mononuclear cells (PBMC), MCP-1 and TNFalpha levels are higher in stable untreated MS patients. Interferon gamma (IFNgamma) is higher in relapsing patients in PHA(-) cultures and in stable patients in PHA(+) cultures. Chronic IFNbeta-1b treatment down-regulates TNFalpha, IFNgamma and MCP-1 production except for TNFalpha in relapsing patients. IFNbeta-1b, in vitro, increases MCP-1, TNFalpha and IFNgamma spontaneous production in all patients. Multivariate analysis suggests that MCP-1 production is dependent from clinical status and not from TNFalpha and IFNgamma production. Logistic regression analysis shows that MCP-1 production is significantly modified by treatment. Further studies are needed to clarify the role of MCP-1 in MS.
Iarlori
Carla
C
Department of Oncology and Neuroscience, Neuroimmunology Unit, University "G. d'Annunzio", Via dei Vestini, I-66013, Chieti, Italy.
Reale
Marcella
M
De Luca
Giovanna
G
Di Iorio
Angelo
A
Feliciani
Claudio
C
Tulli
Antonio
A
Conti
Pio
P
Gambi
Domenico
D
Lugaresi
Alessandra
A
D000328:Adult; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology*; D015999:Multivariate Analysis; D012333:RNA, Messenger / Q000032:analysis; D012008:Recurrence
D018932:Chemokine CCL2; D012333:RNA, Messenger; D000068576:Interferon beta-1b; D016899:Interferon-beta; D000068556:Interferon beta-1a