[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping].
OBJECTIVE
To establish EBV-immortalized lymphoblast cell lines from Chinese Fanconi anemia (FA) patients.


METHODS
The establishment of lymphoblast cell lines was by EBV-immortalization and subtyping of the cell lines by cell fusion, mytomycin C (MMC) inhibition analysis and/or immunoprecipitation and Western blot.


RESULTS
(1) Three lymphoblast cell lines were established, two of them were sensitive to MMC with a IC (50) < 10 nmol/L. (2) The subtypes of all the three patients belong to FA-A group.


CONCLUSIONS
Complementation analysis was suitable for all FA patients with MMC-sensitive lymphoblastic cell lines, but this method is time consuming. Immunoprecipitation and Western blot are much faster for subtyping but could only be used for FA patients whose related genes had been cloned and specific antibodies been developed.
Xie
Y
Y
Department of Hematology, The First Affiliated Hospital of Hubei Medical University, Wuhan 430060, China.
Hans
J
J
Peng
G
G
Chen
Y
Y
Chen
L
L
D001402:B-Lymphocytes / Q000187:drug effects / Q000821:virology*; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed / Q000187:drug effects / Q000821:virology*; D005199:Fanconi Anemia / Q000473:pathology* / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin