Protective effect of Clostridium septicum alpha-toxoid vaccine against challenge with spores in guinea pigs.
The protective effect of an alpha-toxoid vaccine of Clostridium septicum purified alpha-toxin was investigated in guinea pigs. Purified alpha-toxin was treated with formalin to make toxoid, and alpha-toxoid vaccine was prepared by mixing alpha-toxoid (4 to 64 microg/dose) with an aluminum phosphate gel as adjuvant. Guinea pigs were immunized twice with different doses of alpha-toxoid vaccine, and challenged with spores of C. septicum. The guinea pigs surviving after challenge had been immunized with 8 microg/dose or more of alpha-toxoid. All these animals produced titers of 20 units or higher of antitoxin at the challenge. The results suggest that C. septicum alpha-toxin plays an important role in protection against challenge with spores in guinea pigs.
Amimoto
Katsuhiko
K
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Uji, Japan.
Ohgitani
Toshiaki
T
Sasaki
Osamu
O
Oishi
Eiji
E
Katayama
Shigeji
S
Isogai
Makoto
M
Ota
Sotoyuki
S
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology*; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D003013:Clostridium / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology* / Q000517:prevention & control; D005260:Female; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D014121:Toxoids / Q000276:immunology* / Q000592:standards; D010738:Type C Phospholipases / Q000276:immunology; D014709:Vero Cells
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D014121:Toxoids; D010738:Type C Phospholipases