Methylation status of oestrogen receptor-alpha gene promoter sequences in human ovarian epithelial cell lines.
We have determined the methylation status of the CpG island of the oestrogen receptor alpha gene in seven human ovarian cell lines. Cell lines expressing oestrogen receptor alpha showed no evidence of hypermethylation. In three of four cell lines that produced no detectable oestrogen receptor alpha protein, hypermethylation was observed at the NotI site of the CpG island. These results indicate that aberrant hypermethylation may be responsible for a significant proportion of epithelial ovarian tumours in which oestrogen receptor alpha expression is lost.
O'Doherty
A M
AM
School of Biology and Biochemistry, The Queen's University of Belfast, Lisburn Road, Belfast, Northern Ireland, UK.
Church
S W
SW
Russell
S E H
SE
Nelson
J
J
Hickey
I
I
D015153:Blotting, Western; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D004273:DNA, Neoplasm; D047628:Estrogen Receptor alpha; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D047628:Estrogen Receptor alpha; D011960:Receptors, Estrogen