[Study on induction of leukemic cell apoptosis by antisense oligodeoxynucleotide of human telomerase RNA].
OBJECTIVE
To explore the antitumor activity of human telomerase RNA antisense oligodeoxynucleotide (As-ODN) to leukemic cells and its mechanisms.


METHODS
As-ODN was transfected into HL-60 cells by liposomal transfection. Telomerase activity of HL-60 cells was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology, DNA agarose gel electrophoresis and flow cytometry.


RESULTS
The telomerase activity in HL-60 cells, being transfected with 0.1 - 2.0 micromol/L of As-ODN for 1 - 6 days, varied from 1.043 +/- 0.045 to 0.063 +/- 0.011 in a dose- and time-dependent manner. The proliferation of As-ODN-transfected HL-60 cells was lower than that of control, and the cells underwent apoptosis. The Ms-ODN (missense ODN)-transfected HL-60 cells didn't show these changes.


CONCLUSIONS
As-ODN can inhibit the telomerase activity of HL-60 cells specifically and induce apoptosis.
Xie
W
W
Department of Hematology, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 310003, China.
Lin
M
M
D017209:Apoptosis; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology*; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D019098:Telomerase / Q000235:genetics* / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D016372:RNA, Antisense; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase