title: [Experimental study on ex vivo expansion of CD(34)(+) umbilical cord blood cells].
abstract: OBJECTIVE
  To investigate the potential possibility that to expanse ex vivo umbilical cord blood (UCB) cells to an amount sufficient adults transplantation.
  
  
  METHODS
  Purified CD(34)(+) UCB cells from 10 fresh UCB samples were cultured for 7 days in IMDM mediums containing 20% FBS and one of the following three combinations: Group A (IL-1beta + IL-3 + IL-6 + G-CSF + Epo + FL), Group B (SCF + IL-1beta + IL-3 + IL-6 + G-CSF + Epo) and Group C (FL + SCF + IL-1beta + IL-3 + IL-6 + G-CSF + Epo).
  
  
  RESULTS
  (1) There was no significant difference between Groups A and B in expansion of UCB cells. But in group C, the expansion was greater than that in group A (P < 0.01) or group B (P < 0.05). The effects of FL and SCF were synergistic. (2) Over 50 x 10(6) CD(34)(+) cells which are sufficient for adult transplantation were obtained in 30% of UCB samples in group C. (3) The expanded CD(34)(+) UCB cells retained original clonogenic efficiency, the primitive CD(34)(+)CD(38)(-) proportion and the expansion potential were the same as fresh UCB cells.
  
  
  CONCLUSIONS
  The ex vivo expansion of CD(34)(+) UCB cells might provide sufficient hematopoietic stem cells for adult transplantation.
authors.affiliation: tute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
authors.forename: L
authors.initials: L
authors.lastname: Qiu
authors.forename: R H
authors.initials: RH
authors.lastname: Herzig
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D007375:Interleukin-1; D007377:Interleukin-3; D015850:Interleukin-6; D019089:Stem Cell Factor; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor