title: Crystallization and preliminary X-ray diffraction studies of a D-lysine-based chiral PNA-DNA duplex.
abstract: A 10-mer duplex formed between a PNA containing a 'chiral box' of three adjacent D-Lys-based monomers and its complementary DNA strand has been crystallized for the first time. Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer. The crystals belong to the space group P3(1) or to its enantiomorph P3(2), with unit-cell parameters a = b = 35.00, c = 35.91 A. A complete data set has been collected at the synchrotron source Elettra in Trieste to 1.85 A resolution, using a single frozen crystal.
authors.affiliation: Centro di Studio di Biocristallografia-CNR, University of Naples Federico II, Via Mezzocannone 6/8, 80134 Naples, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Menchise
authors.forename: Giuseppina
authors.initials: G
authors.lastname: De Simone
authors.forename: Roberto
authors.initials: R
authors.lastname: Corradini
authors.forename: Stefano
authors.initials: S
authors.lastname: Sforza
authors.forename: Nicola
authors.initials: N
authors.lastname: Sorrentino
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
authors.forename: Michele
authors.initials: M
authors.lastname: Saviano
authors.forename: Carlo
authors.initials: C
authors.lastname: Pedone
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D020135:Peptide Nucleic Acids; D011092:Polyethylene Glycols; D004247:DNA; D008239:Lysine