A cationic sub-micron emulsion (MF59/DOTAP) is an effective delivery system for DNA vaccines.
A novel cationic emulsion was developed to adsorb plasmid DNA and improve intracellular delivery of plasmid DNA upon immunization. The emulsion developed, had a highly uniform particle and charge distribution. Based on observations with cationic microparticles, the cationic emulsion was evaluated in vivo in mice and rabbits with a model HIV-1 pCMVp55 gag DNA. In both these species, the cationic emulsion engendered higher antibody responses than those obtained with naked DNA. The cationic emulsion also maintained the cellular responses seen with naked DNA at the same doses.
Ott
Gary
G
Chiron Corporation, Mail Stop 4.3, 4560 Horton Street, Emeryville, CA 94608, USA.
Singh
Manmohan
M
Kazzaz
Jina
J
Briones
Maylene
M
Soenawan
Elawati
E
Ugozzoli
Mildred
M
O'Hagan
Derek T
DT
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D002412:Cations / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000706:statistics & numerical data; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage*; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D011136:Polysorbates / Q000008:administration & dosage*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage*; D011817:Rabbits; D013185:Squalene / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D016915:AIDS Vaccines; D000276:Adjuvants, Immunologic; D002412:Cations; D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; C089950:MF59 oil emulsion; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D019444:Vaccines, DNA; D013185:Squalene; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane