Separation of DNA fragments in hydroxylated poly(dimethylacrylamide) copolymers.
The novel polymer matrices reported here are low-viscosity sieving media for DNA capillary electrophoresis. This new family of matrices comprises copolymers of N,N-dimethylacrylamide with different monomers which increase polymer hydrophilicity. All these new copolymers self-coat on fused-silica capillaries. Resolution, peak spacing and peak width were the parameters taken into account to assess the influence of polymer structure on separation selectivity and efficiency. This work demonstrates that the performance of polydimethylacrylamide (PDMA) can be improved through copolymerization with hydrophilic monomers. The improvement is related to the efficiency parameter. The new copolymers, due to their low viscosity high sieving capacity and ability to suppress EOF, represent a better alternative to PDMA and are suitable replaceable matrices for capillary and microchip electrophoresis.
Chiari
Marcella
M
Institute of Biocatalysis and Molecular Recognition, CNR, Milano, Italy. chiari@ico.mi.cnr.it
Cretich
Marina
M
Consonni
Roberto
R
D000178:Acrylamides / Q000737:chemistry* / Q000493:pharmacokinetics; D020099:Coated Materials, Biocompatible; D004247:DNA / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D057927:Hydrophobic and Hydrophilic Interactions; D013499:Surface Properties; D014783:Viscosity
D000178:Acrylamides; D020099:Coated Materials, Biocompatible; C429790:poly(N,N-dimethylacrylamide); D004247:DNA