[A gene study on multidrug resistance of retinoblastoma].
OBJECTIVE
To investigate the expression of multidrug resistance gene (MDR1) and multidrug associated protein (MRP) gene in the retinoblastoma (Rb) cell line HXO-RB(44), and study the mechanism of multidrug resistance (MDR) phenotype occurring in retinoblastoma.


METHODS
Reverse transcriptase polymerase chain reaction (RT-PCR) was employed to detect the transcription of MDR1 and MRP gene in the HXO-RB(44) cell line, and immunohistochemical technique was used to detect their protein products P-glycoprotein and multidrug resistance associated protein (P190).


RESULTS
Both MDR1 and MRP genes were expressed in HXO-RB(44) cell line, as well as P-glycoprotein and P190 were over-expressed (96% - 97%) in this cell line.


CONCLUSIONS
Over-expression of MDR1 and MRP genes is significantly involved in the mechanism of MDR phenotype in retinoblastoma.
Liu
J
J
Department of Ophthalmology, Xiangya Second Hospital, Certral South University, Changsha 410011, China.
Tang
L
L
Liu
X
X
Zhang
X
X
Gu
H
H
D045744:Cell Line, Tumor; D019450:Genes, MDR / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D027425:ATP-Binding Cassette, Sub-Family C Proteins; D012333:RNA, Messenger