Mitochondrial-type hsp70 genes of the amitochondriate protists, Giardia intestinalis, Entamoeba histolytica and two microsporidians.
Genes encoding putative mitochondrial-type heat shock protein 70 (mit-hsp70) were isolated and sequenced from amitochondriate protists, Giardia intestinalis, Entamoeba histolytica, and two microsporidians, Encephalitozoon hellem and Glugea plecoglossi. The deduced mit-hsp70 sequences were analyzed by sequence alignments and phylogenetic reconstructions. The mit-hsp70 sequence of these four amitochondriate protists were divergent from other mit-hsp70 sequences of mitochondriate eukaryotes. However, all of these sequences were clearly located within a eukaryotic mitochondrial clade in the tree including various type hsp70 sequences, supporting the emerging notion that none of these amitochondriate lineages are primitively amitochodrial, but lost their mitochondria secondarily in their evolutionary past.
Arisue
Nobuko
N
Department of Biosystems Science, Graduate University for Advanced Studies (Sokendai), Hayama, Kanagawa 240-0193, Japan.
Sánchez
Lidya B
LB
Weiss
Louis M
LM
Müller
Miklós
M
Hashimoto
Tetsuo
T
D000595:Amino Acid Sequence; D000818:Animals; D016819:Encephalitozoon / Q000235:genetics; D004748:Entamoeba histolytica / Q000235:genetics*; D016829:Giardia lamblia / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D016814:Microsporidia / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins