A cytochemical method for visualising blood vessels selectively in semithin LR white sections.
Traditional cytochemical techniques for illustrating blood vessels are usually unsuitable for use in high resolution, 0.35 microm-thick LR White sections due to the thinness of the tissue and, if collagenous, high background staining. We report that a modification of the periodic acid-thiocarbohydrazide-silver proteinate-silver enhancement method (PATCH-SP-SE) selectively visualises, with low background, even the smallest blood vessels in semithin LR White sections of highly collagenous tissues such as human parietal peritoneum.
von Ruhland
C J
CJ
Medical Microscopy Sciences, University of Wales College of Medicine, Cardiff, UK.
Newman
G R
GR
D000180:Acrylic Resins; D001808:Blood Vessels / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008867:Microtomy; D010504:Periodic Acid / Q000737:chemistry; D010503:Periodic Acid-Schiff Reaction / Q000379:methods*; D010537:Peritoneum / Q000098:blood supply
D000180:Acrylic Resins; D006834:Hydrazines; D010504:Periodic Acid; C011368:thiocarbohydrazide