Rapid diagnosis of enterovirus infections by real-time PCR on the LightCycler using the TaqMan format.
A 2 h single-tube, reverse-transcription(RT)-PCR/hybridization assay using the TaqMan format for rapid diagnostic screening of enterovirus (EV) infections was optimized for the real-time LightCycler (LC) technology. For low EV load clinical samples an additional 30 min reamplification step using a novel primer-mix/probe design resulted in a 100% concordance with AMPLICOR EV PCR Test and in-house RT-PCR. Combined with maximum specificity, the sensitivity of LC-PCR was 10- to 100-fold higher compared to AMPLICOR EV Test.
Watkins-Riedel
Thomas
T
The Division of Clinical Virology, Institute of Virology and Infectious Diseases, University Hospital Vienna, A-1090, Vienna, Austria. thomas.watkins-riedel@akh-wien.ac.at
Woegerbauer
Markus
M
Hollemann
David
D
Hufnagl
Peter
P
D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000821:virology; D005243:Feces / Q000821:virology; D005456:Fluorescent Dyes; D006801:Humans; D010614:Pharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000378:metabolism*; D013997:Time Factors
D005456:Fluorescent Dyes; D012367:RNA, Viral; D019914:Taq Polymerase