[An experimental study on systemic inflammatory response syndrome induced by subeschar tissue fluid].
OBJECTIVE
To investigate the effects of subeschar tissue fluid (STF) on normal rats and their peritoneal macrophages in vitro, so as to explore its relationship with inflammatory mediators.


METHODS
Eighteen Wistar rats were evenly and randomly divided into three groups, i.e. STF, normal human serum control and blank control groups. The changes of the heart and respiratory rates of rats in every group were recorded by two channel physiological recorder. Blood samples were collected for the determination of hepatic and renal function. The hepatic, pulmonary, renal and cardiac tissues were harvested for routine morphological examination. The changes of the secretion of TNFalpha, IL-1 were also determined.


RESULTS
The heart and respiratory rates increased after the infusion of STF, and leukocyte count increased along with different degrees of injury to lungs, liver and kidneys. It was indicated in in vitro experiment that STF could activate macrophage and stimulate the secretion of TNFalpha and IL-1 from the macrophage.


CONCLUSIONS
STF might induce the development of SIRS in rats, which further lead to the development of MODS.
Chen
J
J
Burn Unit, Nanfang Hospital, First Military Medical University, Guang Zhou, 510515, Guang Dong Provine, P.R. China.
Zhou
Y
Y
Ren
J
J
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D001826:Body Fluids / Q000502:physiology*; D002056:Burns / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D005260:Female; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000503:physiopathology*; D007375:Interleukin-1 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012119:Respiration; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases