Selective removal of circulating immune complexes from patient plasma.
The principle of a patient-specific immunoadsorber (PsIA) is demonstrated. Studies with model systems (HSA/anti-HSA) on immobilization, stability, and leakage form the basis for the presented fast-performance liquid chromatography (FPLC) and batch experiments, which were conducted using two different protein A adsorbers and autologous and heterologous PsIA systems. Experiments to determine the binding capacity of protein A adsorbers and PsIAs are described. In all experiments, the adsorption of plasma IgG, total protein, and C1q and C3d circulating immune complexes were measured. Plasma of patients with autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus) was investigated. Analysis was performed in both the initial plasma and the flow-through or supernatant. Results of the investigations using FPLC and batch experiments were compared. Autologous PsIA systems are suitable for the selective removal of elevated levels of circulating immune complexes in the plasma.
Kunkel
Siegfried
S
R&D Center of Bioengineering GmbH, Rostock/Teterow, Germany. kunkel@cob.bioeng.de
Holtz
Manfred
M
Grossjohann
Beatrice
B
Schuett
Wolfgang
W
Klinkmann
Horst
H
Kong
Deling
D
Yamada
Ryo
R
Kimura
Hajime
H
Matic
Goran B
GB
Ramlow
Wolfgang
W
Boeden
Hans-Friedrich
HF
D000936:Antigen-Antibody Complex / Q000097:blood*; D001781:Blood Component Removal; D002853:Chromatography, Liquid; D006801:Humans; D007074:Immunoglobulin G; D007163:Immunosorbent Techniques; D007164:Immunosorbents
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007164:Immunosorbents