Protective effects of Indigofera tinctoria L. against D-Galactosamine and carbon tetrachloride challenge on 'in situ' perfused rat liver.
The effect of pre-treatment with Indigofera tinctoria (IT) extract against the toxicity of D-Galactosamine (D-GalN) and carbon tetrachloride (CCl4) during 'in situ' perfusion of the liver for 2 hr was studied in rats. Release of LDH and levels of urea in the liver effluent perfusate, was studied and the rate of bile flow was monitored. Perfusion with D-Galactosamine (5 mM) or carbon tetrachloride (0.5 mM) resulted in increased LDH leakage, decreased urea levels in the liver effluent and reduction in bile flow. IT pretreatment (500 mg/kg body weight) in vivo ameliorated D-GalN and CCl4 induced adverse changes towards near normalcy and thereby indicates its hepatoprotective effects in rats.
Sreepriya
M
M
Department of Biochemistry and Molecular Biology, University of Madras, Guindy Campus, Chennai-600 025.
Devaki
T
T
Nayeem
M
M
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D005688:Galactosamine / Q000633:toxicity; D029883:Indigofera; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D010477:Perfusion; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D005688:Galactosamine; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase