Corticotropin-releasing hormone affects cytokine production in human HaCaT keratinocytes.
CRH cutaneous expression is significantly enhanced after exposure to various stimuli (Physiol Rev 2000, 80;979-1020). We evaluated the effect of CRH on cytokine production in HaCaT keratinocytes, a cell line shown to express CRH receptors coupled to cAMP activation and calcium-dependent transmission pathways. It is demonstrated for the first time that exogenously added CRH stimulates production of IL-6 and IL-11. It also inhibits production of IL-1beta and does not affect TNF-alpha production. Our results indicate that CRH function(s) during cutaneous stress may be mediated by differential effects on cytokine production.
Zbytek
Blazej
B
Department of Histology and Immunology, Medical University of Gdansk, Poland.
Mysliwski
Andrzej
A
Slominski
Andrzej
A
Wortsman
Jacobo
J
Wei
Edward T
ET
Mysliwska
Jolanta
J
D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology
D016207:Cytokines; D008070:Lipopolysaccharides; D003346:Corticotropin-Releasing Hormone