[Lung injury caused by passive smoking and its effects on cytokines in rats].
OBJECTIVE
A rat model with chronic bronchitis was replicated by passive inhalation of cigarette smoking fume to study its long-term effects on lung injury and nitric oxide (NO), interluekin-6 (IL-6), interleukin-8 (IL-8).


METHODS
Levels of nitrogen dioxide (NO(2)) and nitrogen trioxide (NO(3)) were measured with spectrophotometry in rats indicating their level of nitric oxide (NO). Levels of IL-6 and IL-8 were determined by enzyme-linked immunosorbent assay (ELISA).


RESULTS
Levels of NO in serum, bronchial alveolar lavage fluid (BALF) and lung tissue in the smoking group were significantly lower than those in the normal controls (P < 0.01). But, levels of IL-6 and IL-8 were higher in the smoking group than those in the controls.


CONCLUSIONS
Long-term passive smoking could cause injury of lung tissue to certain extent, reduction in secretion of NO in endothelial cells and damage to pulmonary vessels.
Pang
B
B
Red Cross Chaoyang Hospital, Beijing 100020, China.
Wang
C
C
Weng
X
X
Tang
X
X
Zhang
H
H
Niu
S
S
Mao
Y
Y
Xin
P
P
Huang
X
X
Zhang
H
H
D000818:Animals; D029481:Bronchitis, Chronic / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D015850:Interleukin-6 / Q000032:analysis*; D016209:Interleukin-8 / Q000032:analysis*; D008168:Lung / Q000473:pathology*; D008297:Male; D009569:Nitric Oxide / Q000032:analysis*; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D015850:Interleukin-6; D016209:Interleukin-8; D014028:Tobacco Smoke Pollution; D009569:Nitric Oxide