Effect of basic fibroblast growth factor on left ventricular geometry in rats subjected to coronary occlusion and reperfusion.
BACKGROUND
Previous studies have demonstrated myocardial salvage by basic fibroblast growth factor administration following chronic myocardial ischemia or acute myocardial infarction.


OBJECTIVE
To study the effect of bFGF on left ventricular morphometry following coronary occlusion and reperfusion episode in rats.


METHODS
bFGF (0.5 mg) or placebo was continuously administered for a period of one week using an implanted osmotic pump. Animals were sacrificed 6 weeks after surgery and myocardial cross-sections were stained with Massontrichrome and with anti-proliferating cell nuclear antigen antibody.


RESULTS
LV area, LV cavity diameter, LV cavity/wall thickness ratio, and injury size were unchanged compared with control animals. Proliferating endothelial cells were significantly more abundant in injured compared with normal myocardium, but with no differences between animals treated or not treated with bFGF.


CONCLUSIONS
One week of systemic bFGF administration following coronary occlusion and reperfusion had no additional effect on LV geometry or cellular proliferation in rats.
Scheinowitz
Mickey
M
Neufeld Cardiac Research Institute, Sheba Medical Center, Tel Hashomer, Israel. mickeys@post.tau.ac.il
Kotlyar
Arkady-Avi
AA
Zimand
Shachar
S
Leibovitz
Ilan
I
Varda-Bloom
Nira
N
Ohad
Dan
D
Goldberg
Iris
I
Engelberg
Santiego
S
Savion
Nafthali
N
Eldar
Michael
M
D043925:Angiogenesis Inducing Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects / Q000502:physiology; D003327:Coronary Disease / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology* / Q000627:therapeutic use*; D006352:Heart Ventricles / Q000187:drug effects* / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects / Q000502:physiology
D043925:Angiogenesis Inducing Agents; D016222:Fibroblast Growth Factor 2