Enhanced expression of iNOS intratumorally and at the immunization site after immunization with IFNgamma-secreting rat glioma cells.
Nitric oxide (NO) can modulate both tumor growth and antitumor immune responses. In order to elucidate the mechanism of curative therapeutic immunization with IFNgamma-producing glioma cells, we examined the expression of inducible nitric oxide synthase (iNOS) in tissue sections from immunized animals. There was a significantly enhanced iNOS expression both intratumorally and at the immunization site. Although the mechanisms behind this dual expression of iNOS most probably are different, our results suggest a role for NO in both the induction and execution of the antitumor response.
Johansson
Anna C
AC
Section for Tumor Immunology, Department of Cell and Molecular Biology, University of Lund, BMC I12, 221 84, Lund, Sweden. Anna.Johansson@wblab.lu.se
Hegardt
Pontus
P
Janelidze
Shorena
S
Visse
Edward
E
Widegren
Bengt
B
Siesjö
Peter
P
D000818:Animals; D001932:Brain Neoplasms / Q000201:enzymology* / Q000276:immunology; D002478:Cells, Cultured; D005910:Glioma / Q000276:immunology*; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000201:enzymology; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D010587:Phagocytosis; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin / Q000201:enzymology; D013154:Spleen / Q000201:enzymology
D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat