The actin loci in the genus Drosophila: establishment of chromosomal homologies among five palearctic species of the Drosophila obscura group by in situ hybridization.
Chromosomal homologies among the four palearctic Drosophila obscura group species D. ambigua, D. tristis, D. obscura, and D. subsilvestris and the "trans-palearctic" species D. bifasciata were established by in situ hybridization using the 5C actin gene of D. melanogaster as a probe. In all species two labeling sites were detected in each of chromosomal elements C and E and one in each of chromosomal elements A and D. In addition one labeling site was detected on element B for the species D. subsilvestris and D. bifasciata. The conservative distribution pattern of the genes of the actin multigene family, the similarities of the locations of the actin genes in the chromosomes of the five species studied, together with the concordant evidence of synteny of visible and other genetic markers as well as the similarities in banding patterns, all agree with the conclusion that the chromosomal elements have retained their essential identity throughout the evolution of these species. Using in situ hybridization detailed information of some homologous regions of chromosomes can also be established.
Bondinas
G P
GP
Department of Genetics, Faculty of Biotechnology, Agricultural University of Athens, Athens, Greece. gbotinas@aua.gr
Loukas
M G
MG
Goulielmos
G N
GN
Sperlich
D
D
D000199:Actins / Q000235:genetics*; D000483:Alleles; D000818:Animals; D002503:Centromere / Q000235:genetics; D002871:Chromosome Banding; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004330:Drosophila / Q000235:genetics* / Q000648:ultrastructure; D019143:Evolution, Molecular; D017403:In Situ Hybridization / Q000379:methods*; D010802:Phylogeny; D012469:Salivary Glands / Q000648:ultrastructure; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D016615:Telomere / Q000235:genetics
D000199:Actins