[Effects of cigarette smoke solution and chrysotile on proliferation activity of human embryo lung cells].
OBJECTIVE
To study the separate and combined effects of cigarette smoke solution and chrysotile on cell proliferation activity.


METHODS
Human embryo lung (HEL)fibroblasts were cultivated with cigarette smoke solution and chrysotile separately or in combination at varied concentrations for certain times, and cell proliferation was observed with [(3)H] TdR incorporation.


RESULTS
Cigarette smoke solution and chrysotile could enhance cell proliferation at lower doses, both separately or in combination, but inhibit it at higher doses. Lower dose of cigarette smoke solution and chrysotile in combination could enhance cell proliferation synergistically, but higher doses could only do additively.


CONCLUSIONS
Cigarette smoke solution and chrysotile could affect HEL cell proliferation separately or in combination, with an enhancing or inhibiting effect, which depended on their doses.
Wang
Q
Q
Department of Occupational Health, Beijing Medical University, Beijing 100083.
Fan
J
J
Liu
S
S
D017632:Asbestos, Serpentine / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008168:Lung / Q000166:cytology*; D012907:Smoking / Q000009:adverse effects*
D017632:Asbestos, Serpentine; D004247:DNA