title: Extensive plasma cell infiltration with crystal IgG inclusions and mutated IgV(H) gene in an osteoarthritis patient with lymphoplasmacellular synovitis. A case report.
abstract: The presence of immunoglobulin crystal inclusions in plasma cells from plasmacytomas and B-NHLs (linked to overstimulation and overproduction) has been frequently reported. Our case describes a lymphoplasmacellular synovitis in a patient with osteoarthritis (OA) showing an unusually high plasma cell infiltration and for the first time crystals in plasma cells. Using immunohistochemistry. these crystals were identified as being IgG with a balanced lambda/kappa ratio. IgV(H) gene analysis (n = 5 clones) showed that they were somatically mutated (R/S of CDR > 3): in one case, an insertion of 9 nucleotides on the CDR2 region was observed. High R/S values in the CDR indicated antigen selectivity and affinity (4/5). Since no germinal centers could be detected and the analyzed B cells showed antigen selectivity, it may be concluded that already antigenically activated B cells migrated into the synovium and locally differentiated into plasma cells, leading to the extensive infiltration observed. Rheumatoid fibroblasts were shown to support terminal B cell differentiation. Our data suggests that the ability of fibroblasts to activate B cells is not only restricted to RA, but also occurs in OA. The intense plasma cell infiltration contributed to further cartilage damage by altering the microenvironment of the nourishing synovial tissue or by the local production of pathogenic autoantibodies.
authors.affiliation: Institute of Pathology, Charité, Humboldt University Berlin, Germany. veit.krenn@charite.de
authors.forename: Raquel
authors.initials: R
authors.lastname: Magalhães
authors.forename: Thorsten
authors.initials: T
authors.lastname: Gehrke
authors.forename: Maria M
authors.initials: MM
authors.lastname: Souto-Carneiro
authors.forename: Jörg
authors.initials: J
authors.lastname: Kriegsmann
authors.forename: Veit
authors.initials: V
authors.lastname: Krenn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002908:Chronic Disease; D003460:Crystallization; D005260:Female; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D002479:Inclusion Bodies / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D020370:Osteoarthritis, Knee / Q000150:complications / Q000235:genetics* / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology*; D013585:Synovitis / Q000209:etiology / Q000235:genetics* / Q000473:pathology
chemical_list: D007074:Immunoglobulin G