Cytochrome c sorption-desorption effects on the external NADH oxidation by mitochondria: experimental and computational study.
The rupture of the outer mitochondrial membrane is known to be critical for cell death, but the mechanism, specifically its redox-signaling aspects, still needs to be studied in more detail. In this work, the external NADH oxidation by rat liver mitochondria was studied under the outer membrane rupture induced by the mitochondria hypotonic treatment or the inner membrane permeability transition. The saturation of the oxidation rate was observed as a function of mitochondrial protein concentration. This effect was shown to result from cytochrome c binding to the mitochondrial membranes. At a relatively high concentration of mitochondria, the oxidation rate was strongly activated by 4 mm Mg(2+) due to cytochrome c desorption from the membranes. A minimal kinetic model was developed to explain the main phenomena of the external NADH oxidation modulated by cytochrome c and Mg(2+) in mitochondria with the ruptured outer membrane. The computational behavior of the model closely agreed with the experimental data. We suggest that the redox state of the released cytochrome c, considered by other authors to be important for apoptosis, may strongly depend on its oxidation by the fraction of mitochondria with the ruptured outer membrane and on the cytoplasmic cytochrome c reductase activity.
Lemeshko
Victor V
VV
School of Physics, Science Department, National University of Colombia, Medellin Branch, AA3840 Medellin, Colombia, South America. vvasilie@perseus.unalmed.edu.co
D000818:Animals; D019295:Computational Biology; D003574:Cytochrome c Group / Q000378:metabolism*; D005470:Fluorometry; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D008956:Models, Chemical; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats
D003574:Cytochrome c Group; D009243:NAD; D008274:Magnesium