title: PCR technique for identification of mussel species.
abstract: Random amplified polymorphic DNA (RAPD) analysis has been applied to the identification of four mussels species: Mytilus edulis, Mytilus chilensis, Mytilus galloprovincialis, and Perna canaliculus. Amplifications of DNA from mussel were carried out using random primers. The most distinctive bands were then isolated, cloned, and sequenced to design specific primers. Finally, DNA from different mussels was amplified with these specific primers, and results allow genetic identification of M. galloprovincialis from the rest of the mussel species.
authors.affiliation: Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Rego
authors.forename: Andrés
authors.initials: A
authors.lastname: Martínez
authors.forename: Ana
authors.initials: A
authors.lastname: González-Tizón
authors.forename: Juan
authors.initials: J
authors.lastname: Vieites
authors.forename: Francisco
authors.initials: F
authors.lastname: Leira
authors.forename: Josefina
authors.initials: J
authors.lastname: Méndez
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000145:classification* / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011786:Quality Control; D015203:Reproducibility of Results; D017747:Seafood
chemical_list: D017931:DNA Primers; D004247:DNA