Selection of Gongronella butleri strains for enhanced chitosan yield with UV mutagenesis.
This paper describes the selection of Gongronella butleri strains producing higher chitosan yield using UV mutagenesis. We have devised an enzyme-linked immunosorbent assay for the selection of high chitin deacetylase (CDA) yielding strains. Mutant strains M+1, M+2 and M+7 could produce twice the extractable chitosan yield and double the CDA activity, as compared to the wild type strain.
Maw
Theingi
T
Department of Biological Sciences, National University of Singapore, Lower Kent Ridge Road, 117543, Singapore, Singapore
Tan
Teck Koon
TK
Khor
Eugene
E
Wong
Sek Man
SM
D000581:Amidohydrolases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000906:Antibodies; D000941:Antigens / Q000032:analysis; D001709:Biotechnology; D002686:Chitin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D048271:Chitosan; D004797:Enzyme-Linked Immunosorbent Assay; D009090:Mucorales / Q000201:enzymology* / Q000235:genetics*; D016296:Mutagenesis; D014466:Ultraviolet Rays
D000906:Antibodies; D000941:Antigens; D002686:Chitin; D048271:Chitosan; D000581:Amidohydrolases; C019866:chitin deacetylase