D-amino-acid oxidase--an improved production of the enzyme by the yeast Trigonopsis variabilis in a laboratory fermentor.
The cultivation of the yeast Trigonopsis variabilis producing D-amino-acid oxidase (an enzyme participating in the transformation of cephalosporin C into 7-aminocephalosporanic acid for the production of beta-lactam antibiotics) was controlled by changes of dissolved oxygen tension and extended fermentation times. The production technology was optimized on a laboratory scale and scale-up parameters were identified.
Kujan
P
P
Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Prell
A
A
Safár
H
H
Holler
P
P
Plhácková
K
K
Sobotka
M
M
D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D003605:D-Amino-Acid Oxidase; D010100:Oxygen