title: The genomic organization of the open reading frame of the red pigment concentrating hormone gene in the blue crab Callinectes sapidus.
abstract: The open reading frame (ORF) of the gene for the precursor of the octapeptide Red Pigment Concentrating Hormone (RPCH) from the blue crab Callinectes sapidus was cloned by PCR with oligonucleotides targeted to the initiation and the end of the translation coding sequences. A 272 bp intron was characterized between nucleotides 343 and 344 of the reported cDNA, present in the region coding for the last amino acids of the precursor related peptide of RPCH. The intron genomic structure here described is similar to that reported for the gene coding for the Adipokinetic Hormone (AKH) of the grasshopper Schistocerca nitans.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados, I.P.N., México, D. F, Mexico City, Mexico.
authors.forename: Francisco
authors.initials: F
authors.lastname: Martínez-Pérez
authors.forename: Jesús
authors.initials: J
authors.lastname: Valdés
authors.forename: Samuel
authors.initials: S
authors.lastname: Zinker
authors.forename: Hugo
authors.initials: H
authors.lastname: Aréchiga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics*; D007438:Introns / Q000235:genetics; D007447:Invertebrate Hormones / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D016415:Sequence Alignment
chemical_list: D007447:Invertebrate Hormones; D009842:Oligopeptides; D011498:Protein Precursors; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid