Characterization of anti-phosphatidylcholine polyreactive natural autoantibodies from normal human subjects.
In 1990 our group reported a patient with autoimmune hemolytic anemia and high titers of IgM anticardiolipin antibodies that cross-reacted with phosphatidylcholine (PTC). These autoantibodies also recognized bromelain-treated erythrocytes (BrE) and in vitro aged erythrocytes. The epitope exposed with this treatment is PTC. To detect and characterize antiphosphatidylcholine antibodies (anti-PTC) in a normal human population, we studied by ELISA the presence of serum anti-PTC (IgG and IgM) in clinically healthy human subjects. The most representative samples were also studied for IgG or IgM activity against BrE by flow cytometry, rheumatoid factor activity, anti-dsDNA, anti-ssDNA by ELISA and by indirect immunofluorecence (IIF) using HEp-2 line and a healthy human fibroblast strain as substratum. Eighty five percent of sera had IgM anti-PTC and none had IgG. IgM antibodies against BrE were inhibited by PTC micelles (mPTC). Anti-PTC were also inhibited by phosphorylcholine and phosphatidic acid. Aggregated gammaglobulin (AGG) reactivity was inhibited by dsDNA and mPTC. The IgM anti-dsDNA activity was inhibited by soluble dsDNA, AGG and mPTC. All sera gave intermediate filaments pattern by IIF and reacted against purified vimentin by dot blot and Western blot.Our study shows hemolytic IgM anti-PTC present in normal human serum. The main epitope recognized by these autoantibodies is phosphorylcholine. The physicochemical characteristics, crossreactivity with self-antigens and functional properties are typical features of natural autoantibodies.
Cabiedes
Javier
J
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, México. jcabiedesc@conacyt.mx
Cabral
Antonio R
AR
López-Mendoza
A Tamara
AT
Cordero-Esperón
Hugo A
HA
Huerta
M Teresa
MT
Alarcón-Segovia
Donato
D
D000974:Antibodies, Antinuclear / Q000097:blood; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D001324:Autoantigens; D002460:Cell Line; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010713:Phosphatidylcholines / Q000276:immunology*; D012217:Rheumatoid Factor / Q000097:blood; D014746:Vimentin / Q000276:immunology
D000974:Antibodies, Antinuclear; D017152:Antibodies, Antiphospholipid; D001324:Autoantigens; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010713:Phosphatidylcholines; D014746:Vimentin; D012217:Rheumatoid Factor