Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat.
The fungus Fusarium graminearum, a pathogen of both wheat and maize, produces a toxin, deoxynivalenol (DON), that causes disease in livestock. A rapid test for DON in wheat was developed using the principle of fluorescence polarization (FP) immunoassay. The assay was based on the competition between DON and a novel DON-fluorescein tracer (DON-FL2) for a DON-specific monoclonal antibody in solution. The method, which is a substantial improvement over our previous DON FP immunoassay, combined a rapid (3 min) extraction step with a rapid (2 min) detection step. A series of naturally contaminated wheat and maize samples were analyzed by both FP immunoassay and liquid chromatography (HPLC-UV). For wheat the HPLC-UV and FP methods agreed well (linear regression r(2) = 0.936), but for maize the two methods did not (r (2) = 0.849). We conclude that the FP method is useful for screening wheat, but not maize, for DON.
Maragos
Chris M
CM
Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 North University Street, Peoria, Illinois 61604, USA. maragocm@ncaur.usda.gov
Plattner
Ronald D
RD
D002851:Chromatography, High Pressure Liquid; D016231:Fluorescence Polarization Immunoassay / Q000379:methods*; D005506:Food Contamination; D012680:Sensitivity and Specificity; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
D014255:Trichothecenes; C007262:deoxynivalenol