title: Calcium supplementation efficiently reduces lead absorption in suckling rats.
abstract: The effect of calcium supplementation on tissue lead was evaluated in suckling Wistar rats. Such data are not yet available in the literature. The following artificial feeding regimen was used for calcium supplementation: cow's milk by addition of 1%, 3% or 6% Ca as CaHPO(4)x2H(2)O suspension to increase the daily calcium intake about 1.4, 2 or 3 times above control values. Artificial feeding was applied during 7 hr each day for nine consecutive days (from day 6 through 15 after birth). The effect of such treatment on lead absorption and elimination was evaluated in two separate experiments: calcium supplementation during oral lead exposure (as acetate; daily dose 2 mg Pb/kg body wt.; total Pb dose 18 mg/kg body wt.) or after a single intraperitoneal lead administration (5 mg/kg body wt.). At the end of experiments, lead in tissues (liver, kidneys, brain and carcass), and essential elements (Ca, Fe, Zn, Cu) were analysed by atomic absorption spectrometry. Calcium supplementation caused a statistically significant decrease of lead in all tissues of sucklings orally exposed to lead. This decrease was dose-related being about 1.3, 1.5 and 2 times lower in groups supplemented with 1%, 3%, or 6% calcium compared to controls, respectively. Increased calcium intake had no effect on incorporated lead after parenteral lead exposure. Calcium supplementation increased carcass calcium and had no effect on trace elements in tissues, pups' general appearance and body weight gain. It is concluded that higher calcium intake might be a way of efficient reduction of lead absorption during the suckling period.
authors.affiliation: Mineral Metabolism Unit, Biomathematics Unit, Institute for Medical Research and Occupational Health, P.O. Box 291, HR-1001 Zagreb, Croatia. vvarnai@mimi.imi.hr
authors.forename: V M
authors.initials: VM
authors.lastname: Varnai
authors.forename: M
authors.initials: M
authors.lastname: Piasek
authors.forename: M
authors.initials: M
authors.lastname: Blanusa
authors.forename: M M
authors.initials: MM
authors.lastname: Sarić
authors.forename: D
authors.initials: D
authors.lastname: Simić
authors.forename: K
authors.initials: K
authors.lastname: Kostial
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000833:Animals, Suckling; D002136:Calcium, Dietary / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007274:Injections, Intraperitoneal; D007408:Intestinal Absorption / Q000187:drug effects*; D007854:Lead / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D002136:Calcium, Dietary; D007854:Lead