Cholinergic innervation and calretinin-immunoreactive neurones in the hippocampus during postnatal development of the rat brain.
Immunohistochemical study of the cholinergic innervation of the hippocampal calretinin-containing cells was conducted on 28 rat brains of postnatal ages: P0, P4, P7, P14, P21, P30 and P60. Sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and calretinin were analysed using confocal laser-scanning microscope. Obtained data demonstrate that during development as well as in adult species calretinin-containing neurones in the rat hippocampus form sparse synaptic contact with VAChT-ir terminals. It seems probable that cholinergic innervation is not crucial for the functioning of CR-ir cells--probably they remain under the greater influence of a system other than the cholinergic system.
Ludkiewicz
Beata
B
Department of Anatomy and Neurobiology, Medical University of Gdańsk, ul. Debinki 1, 80-211 Gdańsk, Poland.
Wójcik
Sławomir
S
Spodnik
Edyta
E
Domaradzka-Pytel
Beata
B
Moryś
Janusz
J
D000109:Acetylcholine / Q000502:physiology*; D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D064032:Calbindin 2; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology; D002799:Cholinergic Fibers / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development*; D026901:Membrane Transport Proteins; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000276:immunology; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
C576512:Calb2 protein, rat; D064032:Calbindin 2; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D064030:S100 Calcium Binding Protein G; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D000109:Acetylcholine