A structurally deviant member of the Euplotes raikovi pheromone family: Er-23.
Pheromones of Euplotes raikovi form a homologous family of proteins with 37- to 40-amino acid residues, including six cysteines that form three strictly conserved disulfide bridges. The determination of the primary structure of the pheromone Er-23, which was isolated from cells derived from natural populations of E. raikovi that secrete the other known pheromones, has now revealed a novel structure type. The polypeptide chain of this pheromone contains 51 residues, 10 of which are cysteines presumably involved in the formation of five disulfide bridges, and lacks a carboxyl-terminal tail following the last cysteine of the sequence. The elongation of the Er-23 molecule is presumed to result from multiple events of gene duplication starting from an ancestral motif Xxx(2-4)-Cys-Xxx(5-7)-Cys.
Di
Giuseppe Graziano
GG
Dipartimento di Biologia Molecolare Cellulare Animale, University of Camerino, Italy.
Miceli
Cristina
C
Zahn
Ralph
R
Damberger
Fred
F
Wüthrich
Kurt
K
Luporini
Pierangelo
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019075:Electrophoresis, Capillary; D016812:Euplotes / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010675:Pheromones / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D010675:Pheromones; D015800:Protozoan Proteins; C440428:pheromone Er-23, Euplotes raikovi