title: Capsaicin effectively prevents apoptosis in the contralateral testis after ipsilateral testicular torsion.
abstract: OBJECTIVE
  To evaluate the effect of afferent nerve blockage by administration of capsaicin on apoptotic changes in the contralateral testis in rats undergoing ipsilateral testicular torsion.
  
  
  METHODS
  Twenty male albino rats were randomly divided into four groups. In groups 1 and 2, rats underwent a sham operation and testicular torsion, respectively, after the intraperitoneal administration of 0.9% NaCl. Similarly, in groups 3 and 4 the rats underwent a sham operation and testicular torsion, respectively, after an intraperitoneal capsaicin injection. The testes were untwisted 24 h later and the contralateral testes harvested. Apoptosis was assessed in paraffin-embedded sections stained for nuclear DNA fragmentation. Fifteen cells were counted in each seminiferous tubule and the apoptotic cells recorded. A score was calculated for each group and the results compared using the Kruskal-Wallis analysis of variance and Mann Whitney U-tests, with P<0.05 considered to be significant.
  
  
  RESULTS
  The mean apoptotic score of group 2 was significantly higher than that of the other groups. There was no difference between the apoptotic scores of groups 1 and 3, 1 and 4, and 3 and 4.
  
  
  CONCLUSIONS
  Capsaicin effectively prevented apoptosis in the contralateral testes of rats that had undergone testicular torsion.
authors.affiliation: Department of Paediatric Surgery, Pamukkale University Faculty of Medicine, Denizli, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Sarioglu-Buke
authors.forename: S
authors.initials: S
authors.lastname: Erdem
authors.forename: G
authors.initials: G
authors.lastname: Gedikoglu
authors.forename: M
authors.initials: M
authors.lastname: Bingol-Kologlu
authors.forename: F C
authors.initials: FC
authors.lastname: Tanyel
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002211:Capsaicin / Q000627:therapeutic use*; D008297:Male; D011897:Random Allocation; D051381:Rats; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000473:pathology; D013737:Testis
chemical_list: D002211:Capsaicin