Phenolic acids reduce the genotoxicity of acridine orange and ofloxacin in Salmonella typhimurium.
Naturally occurring plant phenolics, p-coumaric acid (PA), caffeic acid (CA), ferulic acid (FA) and gentisic acid (GA) (25-100 nmol/L) had protective effects on acridine orange (AO; 216 mumol/L)- and ofloxacin (3 mumol/L)-induced genotoxicity in Salmonella typhimurium. FA, GA and CA exhibited a significant concentration-dependent protective effect against the genotoxicity of AO and ofloxacin, with the exception of PA, which at all concentrations tested abolished the AO and ofloxacin genotoxicity. UV spectrophotometric measurements showed the interaction of PA, FA, GA and CA with AO but not with ofloxacin; this interaction is obviously responsible for the reduction of AO-induced S. typhimurium mutagenicity. In the case of ofloxacin the antimutagenic effect of PA, FA, GA and CA is assumed to be a result of their ability to scavenge reactive oxygen species (ROS) produced by ofloxacin.
Belicová
A
A
Institute of Cell Biology, Faculty of Science, Comenius University, 811 07 Bratislava, Slovakia. belicova@m2.fedu.uniba.sk
Krizková
L
L
Nagy
M
M
Krajcovic
J
J
Ebringer
L
L
D000165:Acridine Orange / Q000633:toxicity*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005841:Gentisates; D062385:Hydroxybenzoates / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D015242:Ofloxacin / Q000633:toxicity*; D011422:Propionates; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D016587:Antimutagenic Agents; D002109:Caffeic Acids; D003373:Coumaric Acids; D005841:Gentisates; D062385:Hydroxybenzoates; D009153:Mutagens; D011422:Propionates; D015242:Ofloxacin; C004999:ferulic acid; D000165:Acridine Orange; C017616:phenolic acid; C495469:p-coumaric acid; C040048:caffeic acid; C010925:2,5-dihydroxybenzoic acid