Mouse beta-mannosidase: cDNA cloning, expression, and chromosomal localization.
Beta-mannosidase is an exoglycosidase involved in the degradation of N-linked oligosaccharides moieties of glycoproteins. Lack of beta-mannosidase activity leads to the lysosomal disorder beta-mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse beta-mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat beta-mannosidase showed 64%, identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse beta-mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.
Beccari
T
T
Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Italy. tbeccari@unipg.it
Bibi
L
L
Stinchi
S
S
Stirling
J L
JL
Orlacchio
A
A
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000201:enzymology* / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005057:Eukaryotic Cells / Q000201:enzymology*; D019143:Evolution, Molecular; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006041:Goats / Q000235:genetics; D006801:Humans; D016464:Lysosomal Storage Diseases / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000235:genetics* / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014781:Viscera / Q000201:enzymology; D044902:beta-Mannosidase
D018076:DNA, Complementary; D012333:RNA, Messenger; D008361:Mannosidases; D044902:beta-Mannosidase