title: Genetic contribution of the interleukin-10 promoter polymorphism in endometriosis susceptibility.
abstract: OBJECTIVE
  Interleukin-10 (IL-10) is an important immunomodulatory cytokine. The aim of this study was to investigate whether polymorphisms of the IL-10 gene promoter polymorphisms may be responsible in part for genetic susceptibility to endometriosis.
  
  
  METHODS
  Polymorphisms at position -1082 and -592 in the IL-10 promoter region were determined in 196 patients with endometriosis and 160 fertile healthy women by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).
  
  
  RESULTS
  There were no statistically significant differences in the genotype and allele frequencies of IL-10 promoter polymorphism between the endometriosis and control groups. However, when subgrouped according to clinical features, the frequencies of the -592*CC genotype and -592*C allele were significantly increased in patients with autoantibodies to carbonic anhydrase II (anti-CA II ab) compared with controls.
  
  
  CONCLUSIONS
  IL-10 promoter polymorphisms were associated with the production of anti-CA II ab in patients with endometriosis, suggesting a role in the genetic susceptibility for endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan. kitawaki@koto.kpu-m.ac.jp
authors.forename: Jo
authors.initials: J
authors.lastname: Kitawaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Obayashi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ohta
authors.forename: Noriko
authors.initials: N
authors.lastname: Kado
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishihara
authors.forename: Hisato
authors.initials: H
authors.lastname: Koshiba
authors.forename: Izumi
authors.initials: I
authors.lastname: Kusuki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Goji
authors.initials: G
authors.lastname: Hasegawa
authors.forename: Naoto
authors.initials: N
authors.lastname: Nakamura
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Hideo
authors.initials: H
authors.lastname: Honjo
mesh_terms: D000328:Adult; D000483:Alleles; D001323:Autoantibodies / Q000097:blood; D024402:Carbonic Anhydrase II / Q000276:immunology; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic
chemical_list: D001323:Autoantibodies; D016753:Interleukin-10; D024402:Carbonic Anhydrase II