Expression of RANTES and CCR1 in oral lichen planus and association with mast cell migration.
BACKGROUND
T lymphocytes and mast cells infiltrate the lamina propria in oral lichen planus (OLP). Chemokines and their receptors are involved in T cell and mast cell migration and accumulation during the inflammatory process.


METHODS
In the present study, we investigated the role of RANTES and its receptors in OLP using immunohistochemistry, RT-PCR and an in vitro chemotaxis assay.


RESULTS
RANTES and CCR1 were expressed on T cells and mast cells in OLP, while OLP lesional T cell supernatants stimulated CCR1 mRNA expression in a human leukemia mast cell line (HMC-1). TNF-alpha stimulated CCR1, CCR4 and CCR5 mRNA expression in the same cell line. OLP lesional T cell supernatants stimulated HMC-1 migration, which was partly inhibited by anti-RANTES antibody.


CONCLUSIONS
The present study shows, for the first time, the distribution of RANTES and CCR1 in OLP. It is hypothesized that RANTES and CCR1 may play important roles in mast cell trafficking and related events in OLP.
Zhao
Z Z
ZZ
School of Dentistry, The University of Queensland, Brisbane, Australia.
Sugerman
P B
PB
Walsh
L J
LJ
Savage
N W
NW
D002460:Cell Line; D018946:Chemokine CCL5 / Q000096:biosynthesis*; D002633:Chemotaxis / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006801:Humans; D008010:Lichen Planus / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D054389:Receptors, CCR1; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D013601:T-Lymphocytes / Q000378:metabolism; D015854:Up-Regulation
C515300:CCR1 protein, human; D018946:Chemokine CCL5; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D054389:Receptors, CCR1; D019707:Receptors, Chemokine