title: Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray.
abstract: To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP(3)) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP(3)-mediated Ca(2+) signaling is enhanced in these neurons.
authors.affiliation: Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, 105-0001, Tokyo, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Fujiyuki
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shirai
authors.forename: Yuko
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Azusa
authors.initials: A
authors.lastname: Kamikouchi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Fujinawa
authors.forename: Azusa
authors.initials: A
authors.lastname: Kato
authors.forename: Atsumi
authors.initials: A
authors.lastname: Tsujimoto
authors.forename: Takeo
authors.initials: T
authors.lastname: Kubo
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000502:physiology*; D017403:In Situ Hybridization; D000072181:Inositol Polyphosphate 5-Phosphatases; D008969:Molecular Sequence Data; D024521:Mushroom Bodies / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases