Physical map of the linear chromosome of Streptomyces hygroscopicus 10-22 deduced by analysis of overlapping large chromosomal deletions.
The chromosomal DNA of Streptomyces hygroscopicus 10-22, a derivative of strain 5102-6, was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestions with AseI gave 11 fragments with a total length of ca. 7.36 Mb. The AseI sites were mapped by analysis of overlapping chromosomal deletions in different mutants and confirmed by Southern hybridizations using partially digested genome fragments and linking cosmids as probes. PFGE analysis of DNA with and without proteinase K treatment, together with the hybridization results, suggested a linear organization with terminal proteins and large terminal inverted repeats. Some deletion mutants had circular chromosomes.
Pang
Xiuhua
X
Bio-X Life Science Research Center, Shanghai Jiaotong University, Shanghai 200030, China.
Zhou
Xiufen
X
Sun
Yuhui
Y
Deng
Zixin
Z
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003360:Cosmids / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*
C052712:endodeoxyribonuclease VspI; D015252:Deoxyribonucleases, Type II Site-Specific