Binding of the Galanthus nivalis agglutinin to thymocytes reveals alterations in surface glycosylation during T-cell development.
Surface binding of the Galanthus nivalis agglutinin (GNA) to thymocyte subsets has been studied in pigs and rodents by multicolour flow cytometry. In all the species examined, analogous staining profiles have been recorded. Counter-staining with anti-CD3epsilon, anti-CD4 and anti-CD8 monoclonal antibodies (MoAb) revealed that a significant increase of the GNA targets on the cell surface occurred during early thymocyte differentiation and reached its maximum at the level of the CD3loCD4+CD8+ small cortical thymocyte. This was followed by a decrease in the GNA binding capacity upon terminal maturation to the single positive thymocytes. PAGE analysis has revealed a dominant GNA-binding glycoprotein (molar mass approx. 90 kDa) present on thymocyte plasma membranes and absent on the surface of splenic lymphocytes, although both the whole cell lysates from both organs contained GNA ligands of the same size. Our findings are in agreement with previous data showing that immature thymocytes differ from their mature counterparts and peripheral T lymphocytes in the surface glycosylation pattern, and support the hypothesis that lectin-glycoprotein interaction plays a significant role in the cell-to-cell crosstalk in the thymic cortex.
Sinkora
J
J
Institute of Microbiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic.
Kolínská
J
J
Reháková
Z
Z
Cerný
J
J
Doubravská
L
L
D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006031:Glycosylation; D037102:Lectins / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D013552:Swine; D013556:Swine, Miniature; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
D037102:Lectins; D037241:Mannose-Binding Lectins; D008562:Membrane Glycoproteins; D037121:Plant Lectins; C063117:snowdrop lectin