A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ.
A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvImJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.
Tang
Shih-Huey E
SH
Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010-3011, USA.
Silva
Francisco J
FJ
Tsark
Walter M K
WM
Mann
Jeffrey R
JR
D000818:Animals; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D007041:Hypoxanthine Phosphoribosyltransferase; C045073:Cre recombinase; D019426:Integrases