Multidrug resistance mechanisms in chronic lymphocytic leukaemia.
We evaluated the presence of P-glycoprotein (P-gp)-170, multidrug resistance protein (MRP), lung resistance protein (LRP)-56 and Bcl-2 in CD19-positive cells from 100 cases of chronic lymphocytic leukaemia (CLL). P-gp-170 was found in 73% of the CLL cases with no significant difference regarding stage or previous treatment. LRP-56 protein was homogeneously distributed with no differences for stage or treatment. MRP protein was detected at a low level of expression in 49.4% of CLL patients with no differences for stage or treatment. Bcl-2 protein was expressed at a high level in all CLL patients and higher levels were found in the advanced stage. This leads us to conclude that P-gp, MRP, LRP-56 and Bcl-2 are frequently expressed in CLL. P-gp, MRP and LRP are not correlated to stage or previous treatment. Bcl-2 is higher in advanced-stage patients. The clinical and biological significance of these zMDR mechanisms in CLL remains to be fully explained.
Consoli
Ugo
U
Division of Haematology with Bone Marrow Transplantation, University of Catania, Italy. ugo.consoli@tin.it
Santonocito
AnnaMaria
A
Stagno
Fabio
F
Fiumara
Paolo
P
Privitera
Antonella
A
Parisi
Giuseppina
G
Giustolisi
Giada M
GM
Pavone
Biagia
B
Palumbo
Giuseppe A
GA
Di Raimondo
Francesco
F
Milone
Giuseppe
G
Guglielmo
Patrizia
P
Giustolisi
Rosario
R
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis; D018941:Antigens, CD19; D018432:Drug Resistance, Multiple; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000188:drug therapy / Q000378:metabolism*; D008214:Lymphocytes / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018941:Antigens, CD19; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein