N-linked oligosaccharide chains of Sendai virus fusion protein determine the interaction with endoplasmic reticulum molecular chaperones.
The selectivity and individual roles of the N-linked oligosaccharide chains of Sendai virus fusion protein (F protein) in the interaction with endoplasmic reticulum molecular chaperones were investigated by analyses of transient expression of single N-glycosylation mutants and sequential immunoprecipitation. We demonstrated differential interactions depending on the location of the N-linked oligosaccharide chain, and showed that these interactions were correlated with the folding and transport of F proteins. Moreover, mutant F proteins that lacked the specific N-linked oligosaccharide chains required for disulfide bond formation showed increased association with ERp57.
Tamura
Taku
T
Faculty of Agriculture, Iwate University, Ueda, Iwate 020-8550, Morioka, Japan.
Yamashita
Tetsuro
T
Segawa
Hiroaki
H
Taira
Hideharu
H
D002135:Calcium-Binding Proteins / Q000378:metabolism; D037281:Calnexin; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006031:Glycosylation; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007212:Indolizines / Q000494:pharmacology; D007535:Isomerases / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D009154:Mutation; D009844:Oligosaccharides / Q000378:metabolism*; D011233:Precipitin Tests; D019704:Protein Disulfide-Isomerases; D017510:Protein Folding; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054836:Indolizidines
D002135:Calcium-Binding Proteins; D037281:Calnexin; D006360:Heat-Shock Proteins; D007212:Indolizines; D007535:Isomerases; D018832:Molecular Chaperones; D009844:Oligosaccharides; D019704:Protein Disulfide-Isomerases; D014760:Viral Fusion Proteins; C085868:PDIA3 protein, human; C037806:castanospermine; D054836:Indolizidines