title: Cloning of several cDNA segments coding for human liver proteins.
abstract: A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single-stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1-antitrypsin, retinol-binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, FRG.
authors.forename: F
authors.initials: F
authors.lastname: Costanzo
authors.forename: L
authors.initials: L
authors.lastname: Castagnoli
authors.forename: L
authors.initials: L
authors.lastname: Dente
authors.forename: P
authors.initials: P
authors.lastname: Arcari
authors.forename: M
authors.initials: M
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Costanzo
authors.forename: G
authors.initials: G
authors.lastname: Raugei
authors.forename: P
authors.initials: P
authors.lastname: Izzo
authors.forename: T C
authors.initials: TC
authors.lastname: Pietropaolo
authors.forename: L
authors.initials: L
authors.lastname: Bougueleret
authors.forename: F
authors.initials: F
authors.lastname: Cimino
authors.forename: F
authors.initials: F
authors.lastname: Salvatore
authors.forename: R
authors.initials: R
authors.lastname: Cortese
mesh_terms: D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D018076:DNA, Complementary; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D008099:Liver; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA