Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing.
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
Aphasizhev
Ruslan
R
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Sbicego
Sandro
S
Peris
Marian
M
Jang
Sei-Heon
SH
Aphasizheva
Inna
I
Simpson
Agda M
AM
Rivlin
Anatoly
A
Simpson
Larry
L
D000818:Animals; D002852:Chromatography, Ion Exchange; D007891:Leishmania / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D008274:Magnesium / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012313:RNA / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing; D012316:RNA Nucleotidyltransferases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000201:enzymology* / Q000235:genetics
D011994:Recombinant Proteins; D012313:RNA; D012316:RNA Nucleotidyltransferases; C031687:UTP-RNA uridylyltransferase; D008274:Magnesium