title: Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity.
abstract: In the present study we used a murine melanoma model to investigate the effect of the 25-kDa heat shock protein (Hsp25) on natural killer (NK) cytotoxicity. The melanoma lines K1735-C123 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control plasmid. Highly purified interleukin (IL)-2-stimulated DX-5+ NK cells showed enhanced lysis of Hsp25-overexpressing K1735-C123 targets in comparison with controls. In contrast, there was no difference in susceptibility to lysis by purified IL-2-stimulated DX-5+ NK cells between Hsp25-overexpressing and control-transfected K1735-M2 targets. Fluorescence-activated cell sorter analysis revealed that Hsp25 is displayed on the cell surface independently of Hsp25 overexpression and metastatic phenotype. Thus, surface localization of Hsp25 does not correlate with the target cell susceptibility to killing. To sum up, a cytoplasmic overexpression of Hsp25 is associated with an increased susceptibility to lysis by DX-5+ NK cells in the low-metastatic murine melanoma model investigated.
authors.affiliation: Institute of Cancer Research, Vienna, Austria. christian.jantschitsch@akh-wien.ac.at
authors.forename: Christian
authors.initials: C
authors.lastname: Jantschitsch
authors.forename: Franz
authors.initials: F
authors.lastname: Trautinger
authors.forename: Gabriele
authors.initials: G
authors.lastname: Klosner
authors.forename: Andrea
authors.initials: A
authors.lastname: Gsur
authors.forename: Irene
authors.initials: I
authors.lastname: Herbacek
authors.forename: Michael
authors.initials: M
authors.lastname: Micksche
authors.forename: Ingela
authors.initials: I
authors.lastname: Kindås-Mügge
mesh_terms: D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006360:Heat-Shock Proteins; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology*; D008545:Melanoma; D051379:Mice; D008809:Mice, Inbred C3H; D018832:Molecular Chaperones; D009362:Neoplasm Metastasis / Q000276:immunology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D006360:Heat-Shock Proteins; D000949:Histocompatibility Antigens Class II; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins