Acute toxicity of lead on tolerance, oxygen consumption, ammonia-N excretion, and metal accumulation in Penaeus indicus postlarvae.
The estuaries and backwaters that are the potential breeding grounds of penaeid shrimps are subject to heavy metal pollution through industrial effluents and domestic sewage. In the present investigation, laboratory experiments were conducted to study the acute toxicity of lead on tolerance, oxygen consumption, ammonia-N excretion, and metal accumulation in Penaeus indicus postlarvae. Static bioassay tests were employed to determine tolerance limits. Oxygen consumption, ammonia-N excretion, and metal accumulation were determined in postlarvae by exposing them to different concentrations of lead for a period of 48 h. Oxygen consumption measurements were made by using a respiratory chamber equipped with an oxygen electrode and ammonia-N was determined with trione (dichloro-S-triamine 2,4,6(1H,3H,5H-trione)). Accumulation of metal was estimated by wet-ash method. The LC50 value for 96 h was 7.223 ppm and the regression equation Y=4.1638+0.9738X with correlation coefficient of 0.9613 was obtained by probit method. A decrease in oxygen consumption and ammonia-N excretion was observed in postlarvae with increasing concentration of lead. A concentration-dependent accumulation of metal was noticed in these postlarvae. Modifications in O:N ratios of postlarvae suggest that lead accumulation might have altered utilization patterns.
Chinni
Satyavathi
S
Department of Zoology, Andhra University, Visakhapatnam 530 003, India. prabhakararao@mailcity.com
Khan
Ritindra N
RN
Yallapragada
Prabhakara Rao
PR
D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007194:India; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000493:pharmacokinetics* / Q000633:toxicity*; D007928:Lethal Dose 50; D010101:Oxygen Consumption / Q000187:drug effects*; D033561:Penaeidae / Q000187:drug effects* / Q000378:metabolism*; D014018:Tissue Distribution; D023382:Toxicity Tests, Acute; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D007854:Lead; D000641:Ammonia