A Fourier analysis approach for capillary polarimetry.
A new method of fringe interrogation based on Fourier analysis was implemented and tested for a capillary polarimetry detector. It has significant advantages over the previously employed depth of modulation (DOM) approach, including speed and alignment insensitivity. The new and old methods were compared using a set of interference fringes typically used to facilitate nanoliter volume polarimetric determinations. Polarimetric response was calculated with both methods over the range from 0 degrees to 180 degrees. The results were found to be in good agreement with Malus Law and indicate that an fast Fourier transform (fft) could be used for real-time capillary scale polarimetry in a probe volume of 40 nL.
Markov
Dmitry A
DA
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
Swinney
Kelly
K
Norville
Kristin
K
Lu
David
D
Bornhop
Darryl J
DJ
D019075:Electrophoresis, Capillary / Q000379:methods*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D015203:Reproducibility of Results