title: Treatment of ovarian cancer with a tropism modified oncolytic adenovirus.
abstract: Ad5-Delta 24RGD is an adenovirus that is selectively replication competent in cells defective in the Rb/p16 pathway, such as ovarian cancer cells. The fiber of Ad5-Delta 24RGD contains an integrin binding RGD-4C motif, allowing Coxsackie adenovirus receptor-independent infection of cancer cells. Oncolysis of cell lines was similar to that of a wild-type control, and replication in primary tumor material was shown using a novel three-dimensional spheroid model. Finally, an orthotopic murine model of peritoneally disseminated ovarian cancer was used to test i.p. administration to tumor-bearing animals. Injection of the agent resulted in eradication of i.p. disease, whereas control animals expired (P < 0.0001). These results suggest that Ad5-Delta 24RGD could be useful for treatment of ovarian cancer in humans.
authors.affiliation: Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294-3300, USA.
authors.forename: Gerd J
authors.initials: GJ
authors.lastname: Bauerschmitz
authors.forename: John T
authors.initials: JT
authors.lastname: Lam
authors.forename: Anna
authors.initials: A
authors.lastname: Kanerva
authors.forename: Kaori
authors.initials: K
authors.lastname: Suzuki
authors.forename: Dirk M
authors.initials: DM
authors.lastname: Nettelbeck
authors.forename: Igor
authors.initials: I
authors.lastname: Dmitriev
authors.forename: Victor
authors.initials: V
authors.lastname: Krasnykh
authors.forename: Galina V
authors.initials: GV
authors.lastname: Mikheeva
authors.forename: Mack N
authors.initials: MN
authors.lastname: Barnes
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Alvarez
authors.forename: Peter
authors.initials: P
authors.lastname: Dall
authors.forename: Ramon
authors.initials: R
authors.lastname: Alemany
authors.forename: David T
authors.initials: DT
authors.lastname: Curiel
authors.forename: Akseli
authors.initials: A
authors.lastname: Hemminki
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D005260:Female; D015316:Genetic Therapy; D051379:Mice; D016513:Mice, SCID; D009842:Oligopeptides / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000628:therapy*; D018874:Spheroids, Cellular; D014779:Virus Replication
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid