Immobilization of glucose oxidase in polypyrrole/polytetrahydrofuran graft copolymers.
Glucose oxidase (GOD) was immobilized in four different conducting polymer matrices, namely: polypyrrole, (PPy), poly(pyrrole-graft-polytetrahydrofuran), (1) and (3); and poly(pyrrole-graft-polystyrene/polytetrahydrofuran), (2). The kinetic parameters V(max) and K(m), and the optimum temperature were determined for both immobilized and native enzymes. The effect of electrolysis time and several supporting electrolytes, p-toluenesulfonic acid, p-toluene sulfonic acid (PTSA), sodium p-toluene sulfonate, sodium p-toluene sulfonate (NaPTS), and sodium dodecyl sulfate, sodium dodecyl sulfate (SDS), on enzyme immobilization were investigated. The high K(m) value (59.9 mM) of enzyme immobilized in PPy was decreased via immobilization in graft copolymer matrices of pyrrole. V(max), which was 2.25 mM/min for pure PPy, was found as 4.71 mM/min for compound (3).
Tirkeş
S
S
Department of Chemistry, Middle East Technical University, Ankara, Turkey.
Toppare
L
L
Alkan
S
S
Bakir
U
U
Onen
A
A
Yağci
Y
Y
D004572:Electrolysis; D004573:Electrolytes; D005663:Furans / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D004573:Electrolytes; D005663:Furans; D011108:Polymers; D011137:Polystyrenes; D011758:Pyrroles; C464913:poly(pyrrole-graft-polystyrene-polytetrahydrofuran); C464912:poly(pyrrole-graft-polytetrahydrofuran); D005949:Glucose Oxidase