SP1 and AP-1 elements direct chromatin remodeling in SV40 chromosomes during the first 6 hours of infection.
To identify the SV40 regulatory sequences responsible for the chromatin remodeling associated with early transcription, SV40 chromosomes containing potential remodeling sequences inserted adjacent to a reporter region were isolated at various times within the first 6 h of infection and analyzed by a combination of restriction endonuclease digestion and competitive PCR amplification. The sequences analyzed included the early domain, the enhancer, the late domain, the early phasing element, the AP-1 element, two tandem copies of the SP1 element, and the AP-4 element. From 30 min to 3 h postinfection only the enhancer, the AP-1 element, and the two tandem copies of the SP1 element caused a change in nuclease sensitivity consistent with chromatin remodeling. These results suggest that the changes in chromatin structure seen in the promoter during activation of early transcription are most likely a result of remodeling by the AP-1 and/or SP1.
Milavetz
Barry I
BI
Department of Biochemistry and Molecular Biology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58202-9037, USA.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002843:Chromatin / Q000378:metabolism*; D002875:Chromosomes / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D007668:Kidney; D027601:Polyomavirus Infections / Q000821:virology; D013539:Simian virus 40 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014412:Tumor Virus Infections / Q000821:virology; D014771:Virion / Q000378:metabolism
D002843:Chromatin; D016329:Sp1 Transcription Factor; D018808:Transcription Factor AP-1; D004262:DNA Restriction Enzymes