Effect of metronidazole on spermatogenesis and FSH, LH and testosterone levels of pre-pubertal rats.
Metronidazole, a 5-nitroimidazole drug has been reported to decrease testicular weight, testicular and epididymal spermatid counts and causes abnormal sperm morphology with degeneration of seminiferous tubules with 6 weeks treatment of metronidazole (400 mg/kg, day). In contrast to DNA flow cytometry (FCM), the histological and gravimetric parameters do not allow a rapid, sensitive, objective and multiparameteric evaluation of reproductive toxicants on spermatogenesis. Moreover, the exact mechanisms for such an effect are not entirely clear. The present study was therefore undertaken to assess the effects of intraperitoneal (i.p.) administration of metronidazole 400 mg/kg daily for 30 days on testicular germ cell changes assessed by DNA (FCM) and hormone levels of testosterone, FSH and LH in pre-pubertal rats. A significant reduction in the haploid cell population in metronidazole treated groups as compared to saline treated controls was observed. The mean serum FSH, LH and testosterone value were also lowered in treated animals. Thus, the spermatotoxic effects of metronidazole were probably mediated by decrease in the circulating hormones responsible for spermatogenesis.
Grover
J K
JK
Department of Pharmacology, All India Institute of Medical Sciences, New Delhi. jkgrover@hotmail.com
Vats
V
V
Srinivas
M
M
Das
S N
SN
Jha
P
P
Gupta
D K
DK
Mitra
D K
DK
D000818:Animals; D000890:Anti-Infective Agents / Q000633:toxicity*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008795:Metronidazole / Q000633:toxicity*; D051381:Rats; D012741:Sexual Maturation; D013091:Spermatogenesis / Q000187:drug effects*; D013737:Testis / Q000166:cytology / Q000187:drug effects; D013739:Testosterone / Q000097:blood
D000890:Anti-Infective Agents; D008795:Metronidazole; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone