title: Molecular motors: single-molecule recordings made easy.
abstract: A fusion protein of kinesin and gelsolin binds a short actin filament which can be visualized using a standard fluorescence microscope. This technique has provided new insight into the mechanism of kinesin action, and in principle it can be extended to allow single-molecule assays of any protein.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Diez
authors.forename: William R
authors.initials: WR
authors.lastname: Schief
authors.forename: Jonathon
authors.initials: J
authors.lastname: Howard
mesh_terms: D018260:Gelsolin / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D010590:Phalloidine / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry
chemical_list: D018260:Gelsolin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D049452:Green Fluorescent Proteins; D010590:Phalloidine; D016547:Kinesins