title: Cloning of the mRNA of overexpression in colon carcinoma-1: a sequence overexpressed in a subset of colon carcinomas.
abstract: We have identified a novel human cDNA overexpressed in a colon carcinoma cell line, TC7, established from a tumor with a normal karyotype arising in a patient with a hereditary nonpolyposis colorectal carcinoma. The OCC-1 (overexpressed in colon carcinoma-1) gene is composed of six exons and located in the q24.1 region of chromosome 12. It is transcribed as two mRNA species that differ in their 5'- and 3'-terminal ends. Abundant accumulation of both transcripts was found in placenta, skeletal muscle, kidney, and pancreas tissues. Absent or very faint expression was observed in heart, brain, lung and liver tissues. Overexpressed in colon carcinoma-1 cDNA direct in vitro translation of several polypeptides whose size is shorter than 9 kDa. Attempts to produce antibodies against these synthesized polypeptides in Escherichia coli failed. The absence of sequences at the mRNA and DNA levels hybridizing with mouse sequences together with the absence of a large open reading frame raise the possibility that OCC-1 sequences appeared recently in evolution and are transcribed as two noncoding regulatory RNA. Elevated levels of OCC-1 mRNA were observed in three of eight colon carcinomas as compared to normal mucosa of the same patient. Since these tumors shared the same characteristics of having a near diploid karyotype, OCC-1 overexpression may be a hallmark of this subset of colon carcinomas.
authors.affiliation: Laboratoire d'Oncogenèse, UMR147 CNRS, Institut Curie, 26, rue d'Ulm, 75248, Cedex 05, Paris, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Pibouin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Villaudy
authors.forename: Didier
authors.initials: D
authors.lastname: Ferbus
authors.forename: Martine
authors.initials: M
authors.lastname: Muleris
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Prospéri
authors.forename: Yurgos
authors.initials: Y
authors.lastname: Remvikos
authors.forename: Gérard
authors.initials: G
authors.lastname: Goubin
mesh_terms: D000328:Adult; D000818:Animals; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003110:Colonic Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger