Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.
A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.
Ignatov
Atanas
A
Department of Biology, Saint Louis University, St. Louis, Missouri 63103, USA.
Keath
Elizabeth J
EJ
D001426:Bacterial Proteins / Q000032:analysis*; D002467:Cell Nucleus / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000032:analysis*; D004586:Electrophoresis; D005656:Fungal Proteins / Q000235:genetics; D006658:Histoplasma / Q000737:chemistry* / Q000235:genetics; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000032:analysis
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D014157:Transcription Factors; C087923:Yps-3 protein, Ajellomyces capsulata