title: Analysis of factors associated with low peripheral blood progenitor cell collection in normal donors.
abstract: BACKGROUND
  Predictive factors of the response to rHuG-CSF in normal donors have not been extensively studied.
  
  
  METHODS
  We analyzed factors influencing CD34+ cell yield in the 1st day of collection in 261 healthy donors from the Spanish National Donor Registry. The median age was 38 years (range, 2-72). The median dose of rHuG-CSF was 10 microg per kg per day (range, 5-20) over 4 days. In 103 donors (40%), <4 x 10(6) per kg CD34+ cells were collected. The variables that were analyzed included age, sex, weight, basal complete blood cell count, dose, type of rHuGCSF and schedule of administration, and maximum WBC count before apheresis.
  
  
  RESULTS
  By univariate analysis, the maximum WBC count (<50 vs. >or=50 x 10(9)/L, p = 0.004), advanced age (p = 0.008), and number of daily rHuG-CSF doses (one vs. two; p = 0.01) correlated with the number of CD34+ cells collected. By multivariate analysis, donors age (<38 vs. >or=38 years; p = 0.014) and a single daily dose of rHuG-CSF (p = 0.005) were the two variables that significantly predicted a low CD34+ cell yield.
  
  
  CONCLUSIONS
  Donors' age, with a threshold of 38 years or more, and the rHuG-CSF schedule are the factors that significantly affected CD34+ cell mobilization and collection in healthy donors.
authors.affiliation: Hematology Service, Hospital La Fe, Valencia, Spain. delarubia_jav@gva.es
authors.forename: Javier
authors.initials: J
authors.lastname: de la Rubia
authors.forename: Cristina
authors.initials: C
authors.lastname: Arbona
authors.forename: Felipe
authors.initials: F
authors.lastname: de Arriba
authors.forename: Consuelo
authors.initials: C
authors.lastname: del Cañizo
authors.forename: Salut
authors.initials: S
authors.lastname: Brunet
authors.forename: Concha
authors.initials: C
authors.lastname: Zamora
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Díaz
authors.forename: Joan
authors.initials: J
authors.lastname: Bargay
authors.forename: José
authors.initials: J
authors.lastname: Petit
authors.forename: Javier
authors.initials: J
authors.lastname: de la Serna
authors.forename: Andrés
authors.initials: A
authors.lastname: Insunza
authors.forename: Rosario
authors.initials: R
authors.lastname: Arrieta
authors.forename: María J
authors.initials: MJ
authors.lastname: Pascual
authors.forename: David
authors.initials: D
authors.lastname: Serrano
authors.forename: Isabel
authors.initials: I
authors.lastname: Sanjuan
authors.forename: Ildefonso
authors.initials: I
authors.lastname: Espigado
authors.forename: Adrián
authors.initials: A
authors.lastname: Alegre
authors.forename: Dobleta
authors.initials: D
authors.lastname: Martínez
authors.forename: Amparo
authors.initials: A
authors.lastname: Verdeguer
authors.forename: Carmen
authors.initials: C
authors.lastname: Martínez
authors.forename: Luis
authors.initials: L
authors.lastname: Benlloch
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Sanz
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count; D001835:Body Weight; D001853:Bone Marrow / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011994:Recombinant Proteins; D012737:Sex Factors
chemical_list: D018952:Antigens, CD34; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim