Avian and human influenza a virus strains possess different intracellular nucleoprotein oligomerization efficiency.
We have previously shown (Prokudina-Kantorovich EN and Semenova NP, Virology 223, 51-56, 1996) that the nucleoprotein (NP) of influenza A virus forms in infected cells oligomers which in the presence of SDS and 2-mercaptoethanol (ME) as reducing agent are stable at room temperature (RT) and dissociate at 100 degrees C. Here we report that the efficiency of intracellular NP oligomerization depends on the host origin of influenza A virus strain. Thus, in the cells infected with avian influenza A virus strains the viral NP was almost completely oligomerized and only traces of monomeric NP were detected by polyacrylamide gel electrophoresis (PAGE) in unboiled samples. However, in the cells infected with human influenza A virus strains, besides oligomeric NP also a significant amount of non-oligomerized monomeric NP was detected in unboiled samples. In purified virions of avian and human strains the same difference in NP monomers/oligomers ratio was detected as in the infected cells. A reassortant having all internal protein genes from a human strain and the glycoprotein genes from an avian strain revealed the same intracellular pattern of NP monomers/oligomers ratio as its parental human virus. These findings suggest that the type of NP oligomerization is controlled by the NP gene. The possible connection between the accumulation of protease-sensitive monomeric NP in cells infected with a human influenza strain and the parallel accumulation of cleaved NP in these cells is discussed.
Prokudina
E N
EN
D.I. Ivanovsky Institute of Virology, Moscow, Russia. vpr@zebratelecom.ru
Semenova
N P
NP
Rudneva
I A
IA
Chumakov
V M
VM
Yamnikova
S S
SS
D000818:Animals; D001704:Biopolymers / Q000032:analysis; D002460:Cell Line; D004285:Dogs; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D016865:Reassortant Viruses / Q000378:metabolism; D013045:Species Specificity; D014758:Viral Core Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D001704:Biopolymers; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins