title: Attenuated production of intracellular IL-10 and IL-12 in monocytes from patients with severe asthma.
abstract: Interleukin (IL)-10 and IL-12 production is decreased in peripheral blood mononuclear cells of patients with mild asthma. Using whole blood culture and flow cytometry we examined whether monocyte heterogeneity influenced IL-10 and IL-12 production in subjects with severe asthma. We demonstrated that IL-10 release in LPS-stimulated whole blood culture was decreased in patients with severe persistent asthma compared to those with mild asthma and controls (P = 0.04 and P < 0.001, respectively). In asthmatic patients, the percentage of CD14(+)CD16(+) cells was higher than that from normal subjects (P = 0.04). Severe asthmatics showed significantly less positive staining for IL-10 and IL-12 (P < 0.001 and P = 0.02, respectively) after stimulation in monocytes, compared to mild asthmatics and controls in both CD14(+)CD16(+) and CD14(+)CD16(minus sign) cells. These results suggest that IL-10 synthesis is attenuated in severe persistent asthma compared to mild asthma and that this cannot be explained by the increase in the CD14(+)CD16(+) monocytes in asthma.
authors.affiliation: Department of Thoracic Medicine, National Heart & Lung Institute at Imperial College School of Medicine, Dovehouse Street, London SW3 6LY, United Kingdom.
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Tomita
authors.forename: Sam
authors.initials: S
authors.lastname: Lim
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Hanazawa
authors.forename: Omar
authors.initials: O
authors.lastname: Usmani
authors.forename: Rob
authors.initials: R
authors.lastname: Stirling
authors.forename: K Fan
authors.initials: KF
authors.lastname: Chung
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Barnes
authors.forename: Ian M
authors.initials: IM
authors.lastname: Adcock
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D001769:Blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism*; D008168:Lung / Q000503:physiopathology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D009000:Monocytes / Q000276:immunology*; D017452:Receptors, IgG / Q000378:metabolism*; D012129:Respiratory Function Tests; D012720:Severity of Illness Index
chemical_list: D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D016753:Interleukin-10; D018664:Interleukin-12