title: Alterations of tissue glutathione levels and metallothionein mRNA in rainbow trout during single and combined exposure to cadmium and zinc.
abstract: The objective of this study was to assess the effects of Cd and Zn exposure of rainbow trout (Oncorhynchus mykiss) on (a) hepatic glutathione (GSH) levels; and (b) hepatic and branchial metallothionein (MT) mRNA expression. Juvenile rainbow trout were exposed to waterborne Cd (nominal concentrations: 1.5 or 10 microg Cd l(-1)), Zn (150 or 1000 microg Zn l(-1)) or Cd/Zn mixtures (1.5 microg Cd l(-1) with 200 microg Zn l(-1) or 10 microg Cd l(-1) with 1000 microg Zn l(-1)). After 14 and 28 days of treatment, hepatic concentrations of total glutathione, oxidized glutathione (GSSG) and cysteine were determined by means of fluorometric high performance liquid chromatography (HPLC). Branchial and hepatic expression of MT mRNA was measured by means of semi-quantitative RT-PCR. Exposure of trout to Zn did not result in significantly elevated tissue levels of Zn, whereas Cd accumulation factors changed significantly with time and concentration. Despite of the absence of Zn accumulation, hepatic GSH but not MT mRNA levels were significantly altered in Zn-exposed fish. Cd, on the contrary, affected mainly the MT response but not GSH. Also tissue specific differences in the regulation of the two thiol pools were expressed. The thiol response after exposure to metal mixtures could not be explained by simple addition of the effects of the individual metals. The results indicate that cellular thiol pools show different reaction patterns with respect to specific metals and metal mixtures. Under conditions of long-term, low dose metal exposure, the function of GSH appears to go beyond that of a transitory, first line defense.
authors.affiliation: Department of Chemical Ecotoxicology, UFZ Centre for Environmental Research, Permoserstrasse 15, D-04318 Leipzig, Germany. alange@ruc.ua.ac.be
authors.forename: Anke
authors.initials: A
authors.lastname: Lange
authors.forename: Olivier
authors.initials: O
authors.lastname: Ausseil
authors.forename: Helmut
authors.initials: H
authors.lastname: Segner
mesh_terms: D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D017686:Oncorhynchus mykiss; D009928:Organ Specificity / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein; D005978:Glutathione; D015032:Zinc; D003545:Cysteine; D019803:Glutathione Disulfide