title: Behavioral dysfunction, brain oxidative stress, and impaired mitochondrial electron transfer in aging mice.
abstract: Behavioral tests, tightrope success, and exploratory activity in a T maze were conducted with male and female mice for 65 wk. Four groups were defined: the lower performance slow males and slow females and the higher performance fast males and fast females. Fast females showed the longest life span and the highest performance, and slow males showed the lowest performance and the shortest life span. Oxidative stress and mitochondrial electron transfer activities were determined in brain of young (28 wk), adult (52 wk), and old (72 wk) mice in a cross-sectional study. Brain thiobarbituric acid reactive substances (TBARS) were increased by 50% in old mice and were approximately 15% higher in males than in females and in slow than in fast mice. Brain Cu,Zn-superoxide dismutase (SOD) activity was increased by 52% and Mn-SOD by 108% in old mice. The activities of mitochondrial enzymes NADH-cytochrome c reductase, cytochrome oxidase, and citrate synthase were decreased by 14-58% in old animals. The cumulative toxic effects of oxyradicals are considered the molecular mechanism of the behavioral deficits observed on aging.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Cadiz, 11003 Cadiz, Spain. ana.navarro@uca.es
authors.forename: Ana
authors.initials: A
authors.lastname: Navarro
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Sánchez Del Pino
authors.forename: Carmen
authors.initials: C
authors.lastname: Gómez
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Peralta
authors.forename: Alberto
authors.initials: A
authors.lastname: Boveris
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D021461:Animals, Outbred Strains; D001522:Behavior, Animal / Q000502:physiology*; D001835:Body Weight; D001921:Brain / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008136:Longevity; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D009043:Motor Activity / Q000502:physiology; D009245:NADH Dehydrogenase / Q000378:metabolism; D009469:Neuromuscular Junction / Q000502:physiology; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013569:Synapses / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D013482:Superoxide Dismutase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase