title: Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis.
abstract: The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 A. Native data have been collected to 1.6 A resolution and Hg-derivative data to 2.5 A resolution using a synchrotron-radiation source.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Mowat
authors.forename: David
authors.initials: D
authors.lastname: Leys
authors.forename: Kirsty J
authors.initials: KJ
authors.lastname: McLean
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Rivers
authors.forename: Alison
authors.initials: A
authors.lastname: Richmond
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Munro
authors.forename: Miguel
authors.initials: M
authors.lastname: Ortiz Lombardia
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Alzari
authors.forename: Graeme A
authors.initials: GA
authors.lastname: Reid
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Chapman
authors.forename: Malcolm D
authors.initials: MD
authors.lastname: Walkinshaw
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry*
chemical_list: C464825:cytochrome P-450 CYP121; D003577:Cytochrome P-450 Enzyme System