The healing skin wound: a novel site of action of the chemokine C10.
To gain insight into the molecular mechanisms underlying the wound repair process, we searched for genes that are regulated by skin injury. For this purpose we generated a subtractive cDNA library from normal mouse back skin and 1-day full-thickness excisional wounds. One of the differentially expressed genes encodes the chemokine C10. Using Northern blotting, RNase protection assay and Western blotting, we confirmed the injury-induced expression of C10 at the mRNA and protein level. Maximal levels of C10 mRNA and protein were seen at day 1 after wounding, and expression levels subsequently declined. In situ hybridization and immunohistochemistry revealed expression of C10 in macrophages of the clot and the granulation tissue as well as in keratinocytes of the epidermis and the hair follicles at the wound edge. Since C10 is a potent chemoattractant for macrophages, our results suggest that this chemokine contributes to the strong macrophage influx observed in the healing skin wound.
Kaesler
Susanne
S
Institute of Cell Biology, ETH Zürich, Hönggerberg, CH-8093 Zürich, Switzerland.
Regenbogen
Johannes
J
Durka
Silke
S
Goppelt
Andreas
A
Werner
Sabine
S
D000818:Animals; D019556:COS Cells; D019742:Chemokines, CC / Q000096:biosynthesis / Q000235:genetics*; D002522:Chlorocebus aethiops; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger; D012867:Skin / Q000276:immunology / Q000293:injuries*; D015854:Up-Regulation; D014945:Wound Healing / Q000276:immunology*
D019742:Chemokines, CC; D012333:RNA, Messenger; C070959:Ccl6 protein, mouse