title: Nitric oxide-mediated vasodilatory effect of atrial natriuretic peptide in forearm vessels of healthy humans.
abstract: 1. The aim of the present study was to determine whether the vasorelaxant effect of atrial natriuretic peptide (ANP) is, in part, endothelium dependent in humans. 2. We used veno-occlusive plethysmography to measure forearm blood flow (FBF) during intra-arterial infusions of ANP (4, 8, 16, 32 pmol/min per dL forearm tissue volume) before and after the inhibition of nitric oxide (NO) synthesis by N(G)-monomethyl-L-arginine (L-NMMA; 100 micromol) in seven normal healthy subjects. 3. Atrial natriuretic peptide caused a dose-dependent increase in FBF both before and after L-NMMA and significantly reduced the plasma concentration of angiotensin (Ang) II. Administration of L-NMMA significantly diminished the increase in FBF in response to ANP infusion (P < 0.05). 4. These results suggest that the forearm vasodilative response to ANP is modulated, in part, by an endothelium-derived NO-mediated mechanism associated with a decrease in AngII caused by ANP.
authors.affiliation: The Fourth Department of Internal Medicine, Shimane Medical University, Izumo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugamori
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: Toshio
authors.initials: T
authors.lastname: Shimada
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakane
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Shuzo
authors.initials: S
authors.lastname: Ohata
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kodani
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Kunizawa
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Inoue
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ohta
authors.forename: Ko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shimizu
authors.forename: Harumi
authors.initials: H
authors.lastname: Katoh
authors.forename: Yo
authors.initials: Y
authors.lastname: Murakami
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000804:Angiotensin II / Q000097:blood; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005542:Forearm / Q000098:blood supply*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D018709:Statistics, Nonparametric; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D000804:Angiotensin II; D009569:Nitric Oxide; D009320:Atrial Natriuretic Factor; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III