Cloning of several cDNA segments coding for human liver proteins.
A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single-stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1-antitrypsin, retinol-binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.
Costanzo
F
F
European Molecular Biology Laboratory, Heidelberg, FRG.
Castagnoli
L
L
Dente
L
L
Arcari
P
P
Smith
M
M
Costanzo
P
P
Raugei
G
G
Izzo
P
P
Pietropaolo
T C
TC
Bougueleret
L
L
Cimino
F
F
Salvatore
F
F
Cortese
R
R
D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D018076:DNA, Complementary; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D008099:Liver; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA