Effect of diabetic retinopathy on redox state of aqueous humor and serum albumin in patients with senile cataract.
OBJECTIVE
To investigate the oxidative status of albumin in the aqueous humor and serum of senile cataract patients with diabetes in order to clarify the pathogenesis of this condition.


METHODS
High-performance liquid chromatography (HPLC) was employed to measure the reduced form of albumin (mercaptalbumin) and the oxidized form of albumin (nonmercaptalbumin) in serum and aqueous humor. The mercaptalbumin, nonmercaptalbumin-1, and nonmercaptalbumin-2 fractions in aqueous humor obtained at the start of cataract surgery and in serum obtained intraoperatively were analyzed by HPLC in 7 senile cataract patients with diabetic retinopathy (2 men and 5 women aged 70+/-9.8 years).


RESULTS
The mean content (%) of mercaptalbumin, nonmercaptalbumin-1, and nonmercaptalbumin-2 in serum albumin from the diabetic patients was 60.3+/-7.8, 36.9+/-6.6, and 2.7+/-1.7%, respectively, while the corresponding values for aqueous humor albumin were 40.0+/-14.1, 52.4+/-12.6, and 7.5+/-3.6%. When the mercaptalbumin content (%) of aqueous humor albumin was compared between patients with active and inactive diabetic retinopathy, the respective values were 47.0+/-9.1% and 22.8+/-5.7%. A significant correlation mercaptalbumin content (%) of aqueous humor albumin did not show with the HbA1c level, but there was still a relationship (Y = 5.0 x - 2.7, r = 0.80, and p < 0.052).


CONCLUSIONS
The increase of mercaptalbumin (the reduced form of albumin) in the aqueous humor of patients with diabetic retinopathy probably resulted from an increase of retinal vascular permeability.
Kawai
K
K
Department of Ophthalmology, Ohgaki Municipal Hospital, Gifu, Japan.
Yoh
M
M
Hayashi
T
T
Imai
H
H
Negawa
T
T
Tomida
M
M
Sogami
M
M
Era
S
S
D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000150:complications* / Q000209:etiology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003930:Diabetic Retinopathy / Q000150:complications*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D020033:Protein Isoforms / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human
D006442:Glycated Hemoglobin; D020033:Protein Isoforms; D012709:Serum Albumin; D000075462:Serum Albumin, Human; C001702:mercaptoalbumin; C079732:nonmercaptalbumin