Identification of antigenically distinct populations of Leishmania (Viannia) guyanensis from Manaus, Brazil, using monoclonal antibodies.
Eighty Leishmania isolates from patients who contracted cutaneous leishmaniasis in the Manaus region, Amazonas State, Brazil, were identified as Leishmania (Viannia) guyanensis by the electrophoretic profiles of six enzymes. None reacted with the species-specific monoclonal antibody B19. Two L. (V.) guyanensis subpopulations were detected with the monoclonals B2 and B12. The lack of B19 expression by the L. (V.) guyanensis strains in the present study contrasts with that of the vast majority of the strains of the same parasite from eastern Amazonia and French Guyana that express the epitope.
Romero
Gustavo Adolfo Sierra
GA
Núcleo de Medicina Tropical, Universidade de Brasília, Campus Universitário, Asa Norte. Caixa Postal 04517, DF, 70919-970, Brasilia, Brazil. gromero@unb.br
Ishikawa
Edna
E
Cupolillo
Elisa
E
Toaldo
Cristiane Bentin
CB
Guerra
Marcus Vinitius de Farias
MV
Paes
Marcilene Gomes
MG
Macêdo
Vanize de Oliveira
Vde O
Shaw
Jeffrey J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D001938:Brazil; D004586:Electrophoresis; D000939:Epitopes; D006801:Humans; D018334:Leishmania guyanensis / Q000145:classification* / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes