title: Human herpesvirus-6 rep/U94 gene product has single-stranded DNA-binding activity.
abstract: The characterization is reported of the human herpesvirus-6B (HHV-6B) rep/U94 gene, which is a homologue of the adeno-associated virus type 2 rep. In this study, a monoclonal antibody was produced against HHV-6B REP (anti-REP mAb). Immunofluorescence staining using the anti-REP mAb showed that REP was localized to the nucleus in HHV-6-infected MT4 cells. It was first detected at 24 h post-infection (p.i.) and accumulated to higher levels by 72 h p.i. REP may be expressed only at very low levels in HHV-6-infected cells: even when the late protein glycoprotein H was detected in nearly 90% of HHV-6-infected cells, REP was detected in only a small percentage of them. Western blot analysis showed that the anti-REP mAb recognized a 56-kDa polypeptide in HHV-6B-infected MT4 cells. Furthermore, the REP protein was shown to bind single-stranded DNA.
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Panadda
authors.initials: P
authors.lastname: Dhepakson
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Yun Bao
authors.initials: YB
authors.lastname: Jiang
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Hong Lan
authors.initials: HL
authors.lastname: Huang
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Pilailuk
authors.initials: P
authors.lastname: Akkapaiboon
authors.affiliation: Department of Bacteriology, Hyogo College of Medicine, Hyogo, Japan2.
authors.forename: Toshiomi
authors.initials: T
authors.lastname: Okuno
authors.affiliation: Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
authors.forename: Koichi
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins; D015654:Herpesvirus 6, Human / Q000235:genetics*; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D018411:Spodoptera; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases