A centrifuge-less plasma separation method from whole blood anticoagulated with EDTA-2K for the use of clinical laboratory tests.
In the modern medical laboratory system, simple and rapid processing of specimens are required. In the current system with the transportation line, its centrifugation part would disturb smooth flow of the testing because it needs much time for the centrifugation. To solve the problems, a serum separation method was tried for the whole blood specimen using poly-L-lysine, concanavalin A and phyto-hemoagglutinin. Ploy-L-lysine with molecular weight 130,000 to 210,000 in a final concentration of 0.1% could accelerate blood sedimentation, although its supernatant contaminated platelets. Concanavalin and phytohemoagulutinin could accelerate the sedimentation and obtained plasma, but the method could yield enough amount of supernatant by 1 hour standing. As the purpose of this study is to develop a centrifugeless method, a sieve method using a steel mesh and a magnet was applied to the mixture of EDTA blood, red-cell adhesives and thrombin. The method was unique to separate plasma, but the yield was not so high and chemistry data were not fitted with serum data in some of tests. Thus, the trial would be a new technology, but it was judged that some further improvement will be needed technically.
Tatsumi
N
N
Department of Clinical & Laboratory Medicine, Osaka City University Medical School, Osaka, Japan.
Tsuda
I
I
Fukumori
T
T
Hino
M
M
Takubo
T
T
Kondo
H
H
D000925:Anticoagulants; D001777:Blood Coagulation; D002469:Cell Separation; D002498:Centrifugation; D019411:Clinical Laboratory Techniques; D004492:Edetic Acid; D006801:Humans; D010949:Plasma / Q000166:cytology*
D000925:Anticoagulants; D004492:Edetic Acid