title: Multivariate analysis of determinants of bacterial contamination of whole-blood donations.
abstract: OBJECTIVE
  Introduction of bacteria into blood components at the collection stage seems to be a frequent occurrence. We therefore assessed determinants of bacterial contamination of whole-blood donations to gain insight into contamination mechanisms and direct prevention.
  
  
  METHODS
  A cross-sectional study was carried out on donors accepted for whole-blood donation in four French blood banks. Each blood bank used its own two-stage procedure for phlebotomy site preparation. Contamination was identified by culturing two 15-ml samples (collected aseptically at the outset of donation) in a BacT/Alert 240 system. Determinants were assessed by logistic regression analysis.
  
  
  RESULTS
  Bacterial contamination, mainly by skin flora, occurred in 76 (2.2%) out of 3385 donations. Significant determinants were as follows: the blood bank (odds ratio [OR] range = 3.0-5.6, P < 0.001); lack of repetition of scrub (OR = 2.7, P = 0.032); and donor age > 35 years (OR = 1.8, P = 0.036).
  
  
  CONCLUSIONS
  Systematic scrub repetition should be implemented to reduce bacterial contamination by skin flora at the collection stage. Further research is required to clarify the role of different antiseptic agents and of donor age.
authors.affiliation: Institut de Santé publique, d'Epidémiologie et de Développement, Université Victor Segalen Bordeaux 2, Bordeaux, France. Paul.Perez@isped.u-bordeaux2.fr
authors.forename: P
authors.initials: P
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Bruneau
authors.forename: M
authors.initials: M
authors.lastname: Chassaigne
authors.forename: L R
authors.initials: LR
authors.lastname: Salmi
authors.forename: L
authors.initials: L
authors.lastname: Noel
authors.forename: P
authors.initials: P
authors.lastname: Allouch
authors.forename: A
authors.initials: A
authors.lastname: Audurier
authors.forename: C
authors.initials: C
authors.lastname: Gulian
authors.forename: G
authors.initials: G
authors.lastname: Janus
authors.forename: G
authors.initials: G
authors.lastname: Boulard
authors.forename: P
authors.initials: P
authors.lastname: de Micco
mesh_terms: D000328:Adult; D001769:Blood / Q000382:microbiology*; D001782:Blood Donors; D001793:Blood Preservation; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards*; D003430:Cross-Sectional Studies; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D008297:Male; D015999:Multivariate Analysis; D012867:Skin / Q000382:microbiology