Identification of genes expressed preferentially in the honeybee mushroom bodies by combination of differential display and cDNA microarray.
To clarify the molecular basis underlying the neural function of the honeybee mushroom bodies (MBs), we identified three genes preferentially expressed in MB using cDNA microarrays containing 480 differential display-positive candidate cDNAs expressed locally or differentially, dependent on caste/aggressive behavior in the honeybee brain. One of the cDNAs encodes a putative type I inositol 1,4,5-trisphosphate (IP(3)) 5-phosphatase and was expressed preferentially in one of two types of intrinsic MB neurons, the large-type Kenyon cells, suggesting that IP(3)-mediated Ca(2+) signaling is enhanced in these neurons.
Takeuchi
Hideaki
H
Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, 105-0001, Tokyo, Japan.
Fujiyuki
Tomoko
T
Shirai
Kenichi
K
Matsuo
Yuko
Y
Kamikouchi
Azusa
A
Fujinawa
Yumi
Y
Kato
Azusa
A
Tsujimoto
Atsumi
A
Kubo
Takeo
T
D000818:Animals; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D017344:Genes, Insect / Q000502:physiology*; D017403:In Situ Hybridization; D000072181:Inositol Polyphosphate 5-Phosphatases; D008969:Molecular Sequence Data; D024521:Mushroom Bodies / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000235:genetics
D018076:DNA, Complementary; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases