title: Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.
abstract: For protein-DNA complex crystallization, the choice of the DNA fragment is crucial. With the aim of crystallizing the 31 kDa Fpg DNA-repair enzyme bound to DNA, oligonucleotide duplexes varying in length, sequence, end type and nature of the specific DNA target site were used. Crystals of several protein-DNA combinations grew from solutions containing both polyethylene glycol and salt. This systematic crystallization screening followed by optimization of the crystallization conditions by microseeding led to crystals of Fpg bound to a 13 base-pair duplex DNA carrying the 1,3-propanediol abasic site analogue which are suitable for crystallographic analysis. Complete native data sets have been collected to 2.1 A resolution.
authors.affiliation: Centre de Biophysique Moléculaire UPR4301, affiliated to the University of Orléans, CNRS, Rue Charles Sadron, 45071 Orléans CEDEX 02, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Pereira de Jésus
authors.forename: Laurence
authors.initials: L
authors.lastname: Serre
authors.forename: Nadège
authors.initials: N
authors.lastname: Hervouet
authors.forename: Véronique
authors.initials: V
authors.lastname: Bouckson-Castaing
authors.forename: Charles
authors.initials: C
authors.lastname: Zelwer
authors.forename: Bertrand
authors.initials: B
authors.lastname: Castaing
mesh_terms: D019757:Carbon-Oxygen Lyases / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043382:DNA-Formamidopyrimidine Glycosylase; D005663:Furans / Q000737:chemistry; D013294:Lactococcus lactis / Q000737:chemistry*; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D005663:Furans; D011409:Propylene Glycols; C018674:tetrahydrofuran; D004247:DNA; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase