[Changes of glucocorticoid receptor in cerebral and hepatic cytosol during decompression stress injury in rats].
Objective. To observe the changes of glucocorticoid receptor (GR) in cerebral and hepatic cytosol during decompression stress injury in rats. Method. 30 rats were divided into 5 group. They were placed into the compression chamber for compression and decompression. The binding capacity of GR of cerebral and hepatic cytosol were measured by the exchange assay, using 3H dexamethasone as the ligand. Meanwhile, decompression bubbles on pericardial area were measured using Doppler ultrasonic method. Result. The binding capacity of GR of cerebral and hepatic cytosol reduced after decompression stress injury in the animals, especially cerebral cytosol (P<0.01, P<0.05). The result also showed that the binding capacity of cerebral and hepatic GR should have further decreased, if the therapeutic measure had not been used in animals suffered from decompression sickness (DCS). Conclusion. The changes of the binding capacity of GR of cerebral and hepatic cytosol were proved to be related to decompression stress injury, which might be taken as one of the indices for evaluating injury degree of DCS.
Lin
S L
SL
Naval Medical Research Institute of PLA, Shanghai, China.
Liu
J C
JC
Tan
J X
JX
Chen
Y
Y
Wu
J X
JX
Xiang
F
F
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D003664:Decompression / Q000009:adverse effects; D003665:Decompression Sickness / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism*; D004618:Embolism, Air / Q000000981:diagnostic imaging / Q000209:etiology; D005110:Extracellular Space; D008099:Liver / Q000378:metabolism*; D010496:Pericardium / Q000000981:diagnostic imaging; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D018608:Ultrasonography, Doppler
D011965:Receptors, Glucocorticoid