Crystallization and preliminary X-ray crystallographic studies of a complex between the Lactococcus lactis Fpg DNA-repair enzyme and an abasic site containing DNA.
For protein-DNA complex crystallization, the choice of the DNA fragment is crucial. With the aim of crystallizing the 31 kDa Fpg DNA-repair enzyme bound to DNA, oligonucleotide duplexes varying in length, sequence, end type and nature of the specific DNA target site were used. Crystals of several protein-DNA combinations grew from solutions containing both polyethylene glycol and salt. This systematic crystallization screening followed by optimization of the crystallization conditions by microseeding led to crystals of Fpg bound to a 13 base-pair duplex DNA carrying the 1,3-propanediol abasic site analogue which are suitable for crystallographic analysis. Complete native data sets have been collected to 2.1 A resolution.
Pereira de Jésus
Karine
K
Centre de Biophysique Moléculaire UPR4301, affiliated to the University of Orléans, CNRS, Rue Charles Sadron, 45071 Orléans CEDEX 02, France.
Serre
Laurence
L
Hervouet
Nadège
N
Bouckson-Castaing
Véronique
V
Zelwer
Charles
C
Castaing
Bertrand
B
D019757:Carbon-Oxygen Lyases / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043382:DNA-Formamidopyrimidine Glycosylase; D005663:Furans / Q000737:chemistry; D013294:Lactococcus lactis / Q000737:chemistry*; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry
D005663:Furans; D011409:Propylene Glycols; C018674:tetrahydrofuran; D004247:DNA; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase