Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD).
AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.
Ha
W Y
WY
Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Shaw
P C
PC
Liu
J
J
Yau
Forrest C F
FC
Wang
J
J
D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D004365:Drugs, Chinese Herbal; D005784:Gene Amplification; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D005894:Panax / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011786:Quality Control
D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal