title: Theileria buffeli infection of a Michigan cow confirmed by small subunit ribosomal RNA gene analysis.
abstract: Theileria buffeli, generally a benign parasite of cattle, has been reported in animals from Texas, Missouri and North Carolina. To date, there have been no reports of the parasite in cattle residing in northern portions of the US. An 8-year-old cow (Maine Anjou x Angus cross-bred) in Michigan presented with hemoglobinuria and a packed cell volume of 9. Blood films stained with Giemsa showed numerous intraerythrocytic parasites morphologically consistent with T. buffeli. The parasite was confirmed to be T. buffeli by SSU rRNA gene sequence analysis (SSU rRNA sequence, Type A). This represents the first report of this parasite in an animal in Michigan.
authors.affiliation: Department of Veterinary Pathobiology, The Texas Veterinary Medical Center, Texas A&M University, College Station, TX 77843-4467, USA.
authors.forename: Raquel
authors.initials: R
authors.lastname: Cossio-Bayugar
authors.forename: Roxanne
authors.initials: R
authors.lastname: Pillars
authors.forename: Jack
authors.initials: J
authors.lastname: Schlater
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Holman
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D005260:Female; D017125:Genes, Protozoan / Q000235:genetics; D008824:Michigan / Q000453:epidemiology; D008969:Molecular Sequence Data; D016053:RNA, Protozoan / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016794:Theileria / Q000235:genetics*; D013801:Theileriasis / Q000453:epidemiology*
chemical_list: D016053:RNA, Protozoan; D012335:RNA, Ribosomal