Live-cell fluorescent biosensors for activated signaling proteins.
A new generation of live-cell fluorescent biosensors enables us to go beyond visualization of protein movements, to quantify the dynamics of many different protein activities. Alternate approaches can report post-translational modifications, ligand interactions and conformational changes, revealing how the location and subtle timing of protein activity controls cell behavior.
Hahn
Klaus
K
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC162, La Jolla, CA 92037, USA. khahn@scripps.edu
Toutchkine
Alexei
A
D000818:Animals; D015374:Biosensing Techniques; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000032:analysis; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis; D011485:Protein Binding / Q000502:physiology; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational / Q000502:physiology; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins