Expression and cellular distribution pattern of plasma membrane calcium pump isoforms in rat pancreatic islets.
This work is aimed at identifying the presence and cellular distribution pattern of plasma membrane calcium pump (PMCA) isoforms in normal rat pancreatic islet. Microsomal fractions of isolated islets and exocrine tissue were analyzed to detect different PMCA isoforms. The cellular distribution pattern of these PMCAs in the islets was also studied in fixed pancreas sections incubated with antibodies against PMCAs and insulin. Antibody 5F10, which reacts with all PMCA variants, showed multiple bands in the blots in the 127-134 kDa region, indicating the presence of several isoforms. Microsomes also reacted positively with specific antibodies for individual PMCA isoforms, generating a band of the expected size. Antibody 5F10 immunocytochemically labeled the plasma cell membrane of both b- and non-b-cells, but predominantly the former. All islet cells were also labeled with antibodies against isoforms 1 and 4, while the antibody reacting with isoform 3 labeled exclusively b-cells. A few b- and non-b-cells were positively labeled with the antibody reacting with PMCA b variant. Negative results were obtained with the antibody against isoform 2. Further studies, together with previous reports on the modulatory effect of insulin secretagogues and blockers upon PMCA activity, may provide evidence of the importance of this particular PMCA expression for islet function under normal and pathological conditions.
García
M E
ME
CENEXA, Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET, PAHO/WHO Collaborating Center), Facultad de Ciencias Médicas, 60 y 120, 1900, La Plata, Argentina.
Del Zotto
H
H
Caride
A J
AJ
Filoteo
A G
AG
Penniston
J T
JT
Rossi
J P F C
JP
Gagliardino
J J
JJ
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000378:metabolism; D046508:Culture Techniques; D007515:Islets of Langerhans / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D053499:Plasma Membrane Calcium-Transporting ATPases; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
C506961:Atp2b2 protein, rat; D027682:Cation Transport Proteins; D007527:Isoenzymes; D053499:Plasma Membrane Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases