Quantification of ephedrines in urine by column-switching high-performance liquid chromatography.
A method for the quantification of five congener ephedrines in urine samples without sample preparation was developed. The analytes were trapped on a C18 precolumn and separated on a C18 BDS analytical column. Baseline separation was achieved for all analytes. The method meets the requirements of the International Olympic Committee (IOC) medical commission regarding cut-off limits for positive doping cases with ephedrines.
Gmeiner
G
G
Chemical Analysis, Austrian Research Center Seibersdorf, Austria. guenter.gmeiner@arcs.ac.at
Geisendorfer
T
T
Kainzbauer
J
J
Nikolajevic
M
M
Tausch
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004809:Ephedrine / Q000652:urine*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D004809:Ephedrine