title: Quantitation of organophosphorus nerve agent metabolites in human urine using isotope dilution gas chromatography-tandem mass spectrometry.
abstract: An isotope dilution gas chromatography-tandem mass spectrometric (GC-MS-MS) method was developed for quantitating the urinary metabolites of the organophosphorus nerve agents sarin, soman, tabun (GA), VX, and GF. Urine samples were concentrated by codistillation with acetonitrile, derivatized by methylation with diazomethane, and analyzed by GC-MS-MS. The limits of detection were less than 4 microg/L for all the analytes except for the GA metabolite, which had a limit of detection of less than 20 microg/L.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA. wjd1@cdc.gov
authors.forename: W Jack
authors.initials: WJ
authors.lastname: Driskell
authors.forename: Ming
authors.initials: M
authors.lastname: Shih
authors.forename: Larry L
authors.initials: LL
authors.lastname: Needham
authors.forename: Dana B
authors.initials: DB
authors.lastname: Barr
mesh_terms: D002138:Calibration; D002619:Chemical Warfare Agents / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds / Q000652:urine*; D011786:Quality Control; D011865:Radioisotope Dilution Technique; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D002619:Chemical Warfare Agents; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds