One step purification of corilagin and ellagic acid from Phyllanthus urinaria using high-speed countercurrent chromatography.
High-speed countercurrent chromatography (HSCCC) has been successfully applied to the preparative separation of corilagin and ellagic acid in one step from the Chinese medicinal plant Phyllanthus urinaria L. by use of direct and successive injections of a crude methanolic extract. Some aspects concerning the practical use of this technique in the described application are considered.
Jikai
Liu
L
Department of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China. jkl@public.km.yn.cn
Yue
Huang
H
Henkel
Thomas
T
Weber
Karlheinz
K
D003377:Countercurrent Distribution / Q000379:methods*; D004610:Ellagic Acid / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D047348:Hydrolyzable Tannins; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D029803:Phyllanthus / Q000737:chemistry*
D005960:Glucosides; D047348:Hydrolyzable Tannins; D004610:Ellagic Acid; C049096:corilagin