Induction of oligodendrocyte-like properties in a primitive hypothalamic cell line by cholesterol, an eye derived growth factor and brain extract.
A serum-free medium has been devised which permits proliferation of the mouse primitive nervous cell line F7. When cholesterol, eye-derived growth factor and brain extract are added in this medium for 48 h, 80-90% of oligodendrocyte-like cells are generated. These cells have diminished substrate adhesion. They acquire the capacity to synthesize carbonic anhydrase II and myelin basic protein, two specific proteins of oligodendrocytes. These observations suggest that F7 clonal cell line, which has been previously shown to be a neurophysin cell precursor, is also a precursor for oligodendrocytes, and represents a bipotent stem cell line for both neuronal and glial cell lineages.
de Vitry
F
F
Groupe de Neuroendocrinologie, Collège de France, Paris.
Delaunoy
J P
JP
Thibault
J
J
Buisson
N
N
Lamande
N
N
Legault
L
L
Delasalle
A
A
Dupouey
P
P
D000818:Animals; D000941:Antigens / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D024402:Carbonic Anhydrase II / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D016895:Culture Media, Serum-Free; D005123:Eye / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology*; D007031:Hypothalamus / Q000166:cytology; D007150:Immunohistochemistry; D051379:Mice; D004676:Myelin Basic Protein / Q000032:analysis; D009836:Oligodendroglia / Q000166:cytology*; D010641:Phenotype; D011863:Radioimmunoassay; D014020:Tissue Extracts / Q000494:pharmacology
D000941:Antigens; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D004676:Myelin Basic Protein; D014020:Tissue Extracts; D002784:Cholesterol; D024402:Carbonic Anhydrase II