title: Immunolocalization of vimentin and alpha-smooth muscle actin in dysfunctional human temporomandibular joint disc samples.
abstract: The expression of vimentin and alpha-smooth muscle (alpha-SM) actin was examined in 10 human temporomandibular joint (TMJ) disc samples, with internal derangement and in two control specimens, in order to evaluate the phenotypic characteristics of TMJ disc cells in relationship to histological findings. This was accomplished by means of monoclonal antibodies specific for vimentin and alpha-SM actin and immunocytochemical technique. The study, revealed that every disc cell constantly expressed vimentin. Scattered alpha-SM actin positive cells could be appreciated in normal TMJ discs and tissues with minor pathological findings. In TMJ discs with severe alterations, i.e. tears and clefts, almost fibroblast-like cells, fibrochondrocytes and chondrocyte-like cells were strongly immunolabelled by anti-alpha-SM actin antibody. According to these findings it can be assumed that vimentin is expressed by all disc cell populations and it appears not to be influenced by any disease condition of the disc; on the other hand the up-regulation alpha-SM actin immunolabelling seems to be correlated to histopathological findings of tears and clefts. Cells, with a contractile phenotype, close to such defects, could be involved in disc tissue contraction and repair. The plasticity of disc cell populations which evolve towards a different phenotype when subjected to action of macro- and micro-environmental factors is also supported.
authors.affiliation: Department of Orthodontics, Policlinico Citta'Universitaria, University of Catania, Viale A. Doria n.6, Catania, Italy. rleonard@mbox.unict.it
authors.forename: R
authors.initials: R
authors.lastname: Leonardi
authors.forename: L
authors.initials: L
authors.lastname: Villari
authors.forename: C
authors.initials: C
authors.lastname: Piacentini
authors.forename: G
authors.initials: G
authors.lastname: Bernasconi
authors.forename: S
authors.initials: S
authors.lastname: Travali
authors.forename: C
authors.initials: C
authors.lastname: Caltabiano
mesh_terms: D000199:Actins / Q000032:analysis*; D000328:Adult; D000911:Antibodies, Monoclonal; D002455:Cell Division; D019902:Chondrocytes / Q000473:pathology; D002863:Chromogenic Compounds; D004396:Coloring Agents; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005456:Fluorescent Dyes; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D004204:Joint Dislocations / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D019224:Temporomandibular Joint Disc / Q000473:pathology*; D013705:Temporomandibular Joint Disorders / Q000473:pathology*; D015854:Up-Regulation; D014746:Vimentin / Q000032:analysis*; D014945:Wound Healing
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D002863:Chromogenic Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D014746:Vimentin