IL-1beta expression in IgM monoclonal gammopathy and its relationship to multiple myeloma.
We have shown that IL-1beta is not detectable in normal plasma cells but is produced by plasma cells from virtually all patients with multiple myeloma (MM). To extend our earlier work, IL-1beta expression was determined in 13 newly diagnosed patients with IgM monoclonal gammopathy. Eleven patients with Waldenstrom's macroglobulinemia (WM) and two patients with IgM MM were investigated for IL-1beta expression by in situ hybridization (ISH). All patients with WM had bone marrow biopsies consistent with the diagnosis, an IgM M-protein in the serum, and subsequently required chemotherapy. Seven of 11 patients with WM had an M-protein >3 g/dl and five patients had bone surveys performed that were negative for osteolytic disease. Two patients were diagnosed with IgM MM because of the presence of significant osteolytic disease on a metastatic bone survey. ISH for kappa, lambda, and IL-1beta expression was performed on bone marrow aspirates from each of the 13 patients. None of the neoplastic cells from the 11 patients with WM showed detectable IL-1beta expression by ISH. However, the neoplastic cells from both patients with IgM MM expressed IL-1beta mRNA at high levels. This aberrant IL-1beta production may explain the presence of bone lesions in the patients with IgM MM.
Donovan
K A
KA
Division of Hematology and Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA.
Lacy
M Q
MQ
Gertz
M A
MA
Lust
J A
JA
D001707:Biopsy, Needle; D001853:Bone Marrow / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007075:Immunoglobulin M; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D008258:Waldenstrom Macroglobulinemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D017931:DNA Primers; D007075:Immunoglobulin M; D007375:Interleukin-1