title: Inhibition of bacterial foodborne pathogens by the lactoperoxidase system in combination with monolaurin.
abstract: The lactoperoxidase system (LPS) and monolaurin (ML) are potential natural antimicrobial agents for use in foods. The LPS is considered to have greatest activity against Gram-negative bacteria while ML is usually considered to have greatest activity against Gram-positive bacteria. An LPS-ML combination system (utilizing lactoperoxidase (LPX) in the range 5-200 mg kg(-1) and ML in the range 50-1,000 ppm) inhibited growth of Escherichia coli O157:H7 and Staphylococcus aureus. Growth of S. aureus was inhibited more strongly in broth than in milk, in milk than in ground beef A similar pattern was observed for E. coli O157:H7, though enhanced inhibition by LPS-ML systems over that obtained in comparable LPS only systems was not observed in ground beef The inhibitory action of the LPS in combination with other lipids was also examined, with progressively weaker inhibition observed in combinations including palmitoleic acid, monopalmitolein, lauric acid, caprylic acid, and sodium lauryl sulphate.
authors.affiliation: Department of Microbiology, University of Otago, Dunedin, New Zealand.
authors.forename: J C
authors.initials: JC
authors.lastname: McLay
authors.forename: M J
authors.initials: MJ
authors.lastname: Kennedy
authors.forename: A L
authors.initials: AL
authors.lastname: Orourke
authors.forename: R M
authors.initials: RM
authors.lastname: Elliot
authors.forename: R S
authors.initials: RS
authors.lastname: Simmonds
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000254:growth & development; D005506:Food Contamination; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000517:prevention & control*; D005989:Glycerides / Q000494:pharmacology*; D006801:Humans; D007784:Lactoperoxidase / Q000494:pharmacology*; D007848:Laurates / Q000494:pharmacology*; D008461:Meat Products / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D050178:Monoglycerides; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D013501:Surface-Active Agents
chemical_list: D005989:Glycerides; D007848:Laurates; D050178:Monoglycerides; D013501:Surface-Active Agents; C020777:monolaurin; D007784:Lactoperoxidase