Interaction between parvovirus NS2 protein and nuclear export factor Crm1 is important for viral egress from the nucleus of murine cells.
A mutation that disrupts the interaction between the NS2 protein of minute virus of mice and the nuclear export factor Crm1 results in a block to egress of mutant-generated full virions from the nucleus of infected murine cells. These mutants produce wild-type levels of monomer and dimer replicative DNA forms but are impaired in their ability to generate progeny single-stranded DNA in restrictive murine cells in the first round of infection. The NS2-Crm1 interaction mutant can be distinguished phenotypically from an NS2-null mutant and reveals a role for the Crm1-mediated export pathway at a late step in viral infection.
Miller
Cathy L
CL
School of Medicine, University of Missouri--Columbia, 65212, USA.
Pintel
David J
DJ
D000818:Animals; D002213:Capsid; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology; D004279:DNA, Viral; D006801:Humans; D028884:Karyopherins / Q000378:metabolism*; D051379:Mice; D008915:Minute Virus of Mice / Q000737:chemistry / Q000502:physiology*; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication; D000097606:Exportin 1 Protein
D004279:DNA, Viral; D028884:Karyopherins; D018160:Receptors, Cytoplasmic and Nuclear; D017361:Viral Nonstructural Proteins; D000097606:Exportin 1 Protein