Genetic manipulation of mouse embryonic stem cells by mutant lambda integrase.
Mutant lambda integrases catalyze site-specific recombination reactions inside mammalian cells. Here we demonstrate that the integrase system can be used to eliminate resistance marker genes from the genome of mouse embryonic stem cells. So-called integrative and excisive recombination pathways led to the precise deletion of the neomycin gene, which was inserted together with a flanking pair of directly repeated recombination sites into the ROSA26 locus by standard targeting techniques. The excision of the resistance gene led to the expression of enhanced green fluorescence protein, which served as a means to sort out cells that had undergone site-specific recombination. Southern analysis and DNA sequencing confirmed that strand exchange reactions had occurred in the genome as expected. Hence, the integrase system may be used in conjunction with other site-specific recombinases as a tool in genome manipulation protocols.
Christ
Nicole
N
Institute of Genetics, University of Cologne, Cologne, Germany.
Dröge
Peter
P
D000818:Animals; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D018014:Gene Transfer Techniques; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D013234:Stem Cells / Q000502:physiology*
D019426:Integrases