Identification of the alternative splice products encoded by the human protein phosphatase inhibitor-1 gene.
We have isolated three major cDNA fragments of protein phosphatase inhibitor-1 from human brain and liver by RT-PCR. The 536 bp fragment encoded the wild-type of inhibitor-1 while two other fragments were alternative splice products of the inhibitor-1 gene, which was confirmed by partial genomic DNA sequencing. The 380 bp fragment encoded an in-frame 51-residue-deleted inhibitor-1, named inhibitor-1alpha, and the deletion occurred from residue 84 to 134 of inhibitor-1. The 316 bp fragment termed inhibitor-1beta was derived from an internal deletion of 536 bp fragment. This deletion resulted in an out of frame shift, allowing the 316 bp fragment that encoded the partial sequence of inhibitor-1. Based on the reported mRNA sequence of inhibitor-1 and evidence from our RT-PCR, we suggested that inhibitor-1beta consisted of 132 amino acids of which the N-terminal 61 amino acid sequences were identical to inhibitor-1 while the sequence after residue-61 was markedly different.
Liu
Hsin-tzu
HT
Department of Research, Buddhist Tzu Chi General Hospital, Hualien 970, Taiwan, Republic of China.
Lin
Ta-hsien
TH
Kuo
Hann-chorng
HC
Chen
Yi-chen
YC
Tsay
Huey-Jen
HJ
Jeng
Hao-Hsuan
HH
Tsai
Pai-Chi
PC
Shie
Fu-kai
FK
Chen
Ji-hsiung
JH
Huang
Hsien-bin
HB
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D011506:Proteins; C450539:PPP1R2C protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1