A misexpression study examining dorsal thorax formation in Drosophila melanogaster.
We studied thorax formation in Drosophila melanogaster using a misexpression screen with EP lines and thoracic Gal4 drivers that provide a genetically sensitized background. We identified 191 interacting lines showing alterations of thoracic bristles (number and/or location), thorax and scutellum malformations, lethality, or suppression of the thoracic phenotype used in the screen. We analyzed these lines and showed that known genes with different functional roles (selector, prepattern, proneural, cell cycle regulation, lineage restriction, signaling pathways, transcriptional control, and chromatin organization) are among the modifier lines. A few lines have previously been identified in thorax formation, but others, such as chromatin-remodeling complex genes, are novel. However, most of the interacting loci are uncharacterized, providing a wealth of new genetic data. We also describe one such novel line, poco pelo (ppo), where both misexpression and loss-of-function phenotypes are similar: loss of bristles and scutellum malformation.
Peña-Rangel
María Teresa
MT
Department of Developmental Neurobiology and Neurophysiology, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro, 76230, México.
Rodriguez
Isabel
I
Riesgo-Escovar
Juan Rafael
JR
D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D010641:Phenotype; D015398:Signal Transduction; D013909:Thorax / Q000002:abnormalities / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D029721:Drosophila Proteins; D014157:Transcription Factors; C109259:pnr protein, Drosophila