Effects of thrombin and of the phospholipase C inhibitor, D609, on the vascularity of the chick chorioallantoic membrane.
Microvascular corrosion casting was used to assess the effects of thrombin and D609, a phospholipase C inhibitor, on the vascularity of the chick embryo chorioallantoic membrane (CAM). Discs containing vehicle, thrombin or D609 were placed on the CAM of fertilized white Leghorn eggs on Day 9 of gestation and vascularity was assessed on Day 11. Thrombin caused significant increases in the numbers (43%), diameters (5%) and lengths (17%), of both pre- and postcapillaries (first-order vessels by centripetal ordering). Conversely, D609 caused a decrease in the numbers (27%), lengths (12%) and diameters (8%) of first-order vessels. D609 decreased the total vascular volume of first- to third-order vessels by 32%, whereas thrombin increased vascular volume by 27%. Additionally, thrombin increased capillary plexus density by 6%, whereas D609 decreased capillary plexus density by 3%. These findings provide a quantitative assessment of changing vascularity in the chick CAM--a model assay system in the development of pro- and antiangiogenic agents.
Dimitropoulou
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Department of Anatomy, University of Mainz, D-55099 Mainz, Germany. cdimitro@mail.mcg.edu
Maragoudakis
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Konerding
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MA
D000482:Allantois / Q000098:blood supply* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D000818:Animals; D001810:Blood Volume / Q000187:drug effects; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D006490:Hemostatics / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009636:Norbornanes; D010063:Ovum / Q000187:drug effects / Q000648:ultrastructure; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D001952:Bridged-Ring Compounds; D006490:Hemostatics; D009636:Norbornanes; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D010738:Type C Phospholipases; D013917:Thrombin