title: In vivo modulation of ETS genes induced by electromagnetic fields.
abstract: We have previously shown that electromagnetic field (EMF) exposure induces ETS1 oncogene overexpression in different cell lines. In order to investigate in vivo EMF effects, BALB/c mice were exposed at different times to 50 MHz radiation, modulated (80%) at 16 Hz. The exposed and control animals were sacrificed and the spleen excised for rt-pcr and western blot analysis. We observed an increase in ETS1 mRNA and protein expression, but a decrease in ETS2 protein levels. Preliminary results from this experimental model show in vivo evidence of the effect of EMF on ETS oncogene expression.
authors.affiliation: Department of Occupational Medicine, I.S.P.E.S.L., Rome, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Mucci
authors.forename: A
authors.initials: A
authors.lastname: Ianni
authors.forename: C L
authors.initials: CL
authors.lastname: Ursini
authors.forename: D
authors.initials: D
authors.lastname: Arzani
authors.forename: N K
authors.initials: NK
authors.lastname: Bhat
authors.forename: P
authors.initials: P
authors.lastname: Navarra
authors.forename: V
authors.initials: V
authors.lastname: Romano-Spica
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D050784:Proto-Oncogene Protein c-ets-1; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C107465:ERF protein, human; C494041:ETS1 protein, human; C494042:Ets1 protein, mouse; C494070:Ets2 protein, mouse; D050784:Proto-Oncogene Protein c-ets-1; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors