title: p16(INK4a) immunocytochemical analysis is an independent prognostic factor in childhood acute lymphoblastic leukemia.
abstract: We investigated the prognostic value of p16(INK4a) immunocytochemistry (ICC) analysis in 126 cases of newly diagnosed childhood acute lymphoblastic leukemia (ALL). The incidence of negative p16(INK4a) ICC was 38.1% and was more frequent in T-lineage ALL. Overall survival (OS) and event-free survival (EFS) were significantly higher in patients with positive p16(INK4a) ICC than in patients with negative ICC (6 years OS, 90% versus 63%, P =.0014; 6 years EFS, 77.8% versus 55%, P =.0033). The p16(INK4a) ICC remained a significant prognostic factor within the subgroup of B-precursor ALL. Multivariate analysis showed that negative p16(INK4a) ICC was an independent prognostic factor for OS (relative risk [RR], 3.38; P =.02) and EFS (RR, 2.49; P =.018). Sequential study showed that p16(INK4a) expression remained stable during first relapse in most patients. These findings indicate that p16(INK4a) ICC is an independent factor of outcome in childhood ALL.
authors.affiliation: Service des Maladies du Sang, Centre Hospitalier et Universitaire Lille, 1 place de Verdun, 59037 Lille, France.
authors.forename: Jean Hughes
authors.initials: JH
authors.lastname: Dalle
authors.forename: Martine
authors.initials: M
authors.lastname: Fournier
authors.forename: Brigitte
authors.initials: B
authors.lastname: Nelken
authors.forename: Françoise
authors.initials: F
authors.lastname: Mazingue
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Laï
authors.forename: Francis
authors.initials: F
authors.lastname: Bauters
authors.forename: Pierre
authors.initials: P
authors.lastname: Fenaux
authors.forename: Bruno
authors.initials: B
authors.lastname: Quesnel
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007223:Infant; D007621:Karyotyping; D008297:Male; D015999:Multivariate Analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000401:mortality / Q000473:pathology*; D011237:Predictive Value of Tests; D011379:Prognosis; D015996:Survival Rate; D013997:Time Factors
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16