Human alkaline phosphatase expression and secretion into chicken eggs after in vivo gene electroporation in the oviduct of laying hens.
In vivo gene electroporation was used to examine whether or not a recombinant protein is synthesized in the chicken oviduct and subsequently secreted into eggs. A plasmid DNA containing a secretion form of the human alkaline phosphatase gene was injected into mucosa of the chicken magnum. Immediately, in vivo gene electroporation was conducted. The human alkaline phosphatase activity in the oviduct mucosa increased and reached its peak at 2 days posttransfection, followed by a sharp decrease to a negligible level at 4 days posttransfection. In the egg white, the alkaline phosphatase activity showed a similar change to that in the magnum mucosa except for a delay of 4 days. The present results imply that in vivo gene electroporation method in the oviduct may serve as a rapid production system of recombinant proteins into chicken eggs.
Takami
Hiroshi
H
Department of Applied Molecular Biosciences, Nagoya University, Nagoya 464-8601, Japan.
Watanabe
Hisako
H
Ohmori
Yasushige
Y
Park
Hyi-Man
HM
Muramatsu
Tatsuo
T
D000256:Adenoviridae / Q000235:genetics; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D018274:Electroporation / Q000379:methods*; D005260:Female; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D010057:Oviducts / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D019076:Transgenes; D001616:beta-Galactosidase / Q000378:metabolism
D012367:RNA, Viral; D011994:Recombinant Proteins; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase