Isolation and characterization of the major nod factor of Bradyrhizobium japonicum strain 532C.
Bradyrhizobium japonicum 532C nodulates soybean effectively under cool Canadian spring conditions and is used in Canadian commercial inoculants. The major lipo-chitooligosaccharide (LCO), bacteria-to-plant signal was characterized by HPLC, FAB-mass spectroscopy MALDI-TOF mass spectroscopy and revealed to be LCO Nod Bj-V (C18:1, MeFuc). This LCO is produced by type I strains of B. japonicum and is therefore unlikely to account for this strains superior ability to nodulate soybean under Canadian conditions. We also found that use of yeast extract mannitol medium gave similar results to that of Bergerson minimal medium.
Soulemanov
A
A
Plant Science Department, McGill University, Quebec, Canada.
Prithiviraj
B
B
Carlson
R W
RW
Jeyaretnam
B
B
Smith
D L
DL
D020369:Bradyrhizobium / Q000737:chemistry / Q000187:drug effects; D002170:Canada; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D013025:Glycine max / Q000254:growth & development / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013057:Spectrum Analysis; D013559:Symbiosis
D003470:Culture Media; D008070:Lipopolysaccharides; C091899:Nod factor V, Bradyrhizobium japonicum; D019833:Genistein