Simultaneous occurrence of myelodysplastic syndrome and monoclonal B lymphocytes with a different clonal origin.
Bone marrow and peripheral blood from a myelodysplastic syndrome patient with trisomy 13 and monoclonal B lymphocytes (without evidence of systemic lymphoma) were investigated for clonal lymphoid lineage involvement using interphase fluorescence in situ hybridization (FISH) and X-chromosome inactivation assay (HUMARA) on CD19+ and CD34+ sorted cells. Trisomy 13 was detected in 55% of CD34+ cells and in 5.5% of CD19+ cells, the latter being comparable to the negative control specimen. X-chromosome inactivation showed both CD34+ and CD19+ cells to be monoclonal, though their inactivated X-chromosome was different. The results strongly suggested that both populations of CD34+ and CD19+ cells have originated from a different progenitor stem cell.
Cauwelier
B
B
Laboratory of Haematology, AZ St. Jan AV, Bruges, Belgium.
Nollet
F
F
De Laere
E
E
Van Leeuwen
M
M
Billiet
J
J
Criel
A
A
Louwagie
A
A
D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology*; D019070:Cell Lineage; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002882:Chromosomes, Human, Pair 13; D002999:Clone Cells / Q000473:pathology; D020732:Cytogenetic Analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D014314:Trisomy