Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris.
SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.
Matsushita
Yasuhiko
Y
Gene Research Center, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan.
Suzuki
Tatsuya
T
Kubota
Ryo
R
Mori
Masako
M
Shimosato
Hiroko
H
Watanabe
Masao
M
Kayano
Toshiaki
T
Nishio
Takeshi
T
Nyunoya
Hiroshi
H
D000595:Amino Acid Sequence; D001937:Brassica / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D010769:Phosphothreonine / Q000378:metabolism; D010940:Plant Proteins; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics
D018076:DNA, Complementary; D010940:Plant Proteins; D010769:Phosphothreonine; D010768:Phosphoserine; D011494:Protein Kinases; C079832:S-receptor kinase; D009701:Nucleoside-Diphosphate Kinase