Characterization of a functional neuropeptide F receptor from Drosophila melanogaster.
Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC(50) = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC(50) = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
Garczynski
Stephen F
SF
Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Brown
Mark R
MR
Shen
Ping
P
Murray
Thomas F
TF
Crim
Joe W
JW
D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017476:Receptors, Neuropeptide Y / Q000145:classification / Q000235:genetics; D016415:Sequence Alignment
D029721:Drosophila Proteins; C499701:NPFR protein, Drosophila; D009479:Neuropeptides; D011956:Receptors, Cell Surface; D018013:Receptors, Neuropeptide; D017476:Receptors, Neuropeptide Y; C400215:neuropeptide F, Drosophila; D000262:Adenylyl Cyclases