Microfluidic chips for clinical and forensic analysis.
This review gives an overview of developments in the field of microchip analysis for clinical diagnostic and forensic applications. The approach chosen to review the literature is different from that in most microchip reviews to date, in that the information is presented in terms of analytes tested rather than microchip method. Analyte categories for which examples are presented include (i) drugs (quality control, seizures) and explosives residues, (ii) drugs and endogenous small molecules and ions in biofluids, (iii) proteins and peptides, and (iv) analysis of nucleic acids and oligonucleotides. Few cases of microchip analysis of physiological samples or other "real-world" matrices were found. However, many of the examples presented have potential application for these samples, especially with ongoing parallel developments involving integration of sample pretreatment onto chips and the use of fluid propulsion mechanisms other than electrokinetic pumping.
Verpoorte
Elisabeth
E
Sensors, Actuators & Microsystems Laboratory, Institute of Microtechnology, University of Neuchâtel, Neuchâtel, Switzerland. sabeth.verpoorte@unine.ch
D000906:Antibodies / Q000032:analysis; D001826:Body Fluids / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D004798:Enzymes / Q000032:analysis; D005554:Forensic Medicine; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006801:Humans; D008832:Microchemistry / Q000379:methods; D010455:Peptides / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity
D000906:Antibodies; D004798:Enzymes; D010455:Peptides; D004364:Pharmaceutical Preparations; D011506:Proteins