[Diagnosis of tuberculosis meningitis by detection of adenosine deaminase activity and amplification of nucleotide sequences with PCR].
Tuberculous meningitis (TBM) is the most severe and lethal form of tuberculosis. The rapid bacteriological diagnosis with the conventional techniques is nearly impossible in TBM. There for many patients are treated with anti-TBC drugs without a definitive diagnosis. A more fast and accurate diagnostic method is necessary, in order to initiate the treatment on time to prevent the irreversible neurologic sequel or death. We evaluated the use of two rapid methods: Adenosine deaminase activity (ADA) and polymerase chain reaction (PCR) for IS6110 and mtp40 sequences on cerebrospinal fluid (CSF) from chronic meningitis patients. For ADA activity > 8.0 U/L the sensibility and specificity was 80% and 91%. PCR sensibility was 80% and specificity 97%. ADA activity and PCR on CSF could be specially useful as complementary tools in the early diagnosis of TBM.
Correa
M F
MF
Servicio de Neurología, Hospital Clínico Universitario, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela, Apartado Postal 50587.
Armas
E
E
Díaz
D
D
de Elguezabal
K
K
De la Rosa
M L
ML
Calles
G
G
Adjounian
H
H
Pedroza
R
R
D000243:Adenosine Deaminase / Q000134:cerebrospinal fluid*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D002908:Chronic Disease; D004796:Clinical Enzyme Tests; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014390:Tuberculosis, Meningeal / Q000175:diagnosis*; D010738:Type C Phospholipases
D001426:Bacterial Proteins; D015415:Biomarkers; D010738:Type C Phospholipases; C101028:MpcA protein, Mycobacterium tuberculosis; D000243:Adenosine Deaminase