14 C-deoxyglucose imaging overestimates myocardial viability in subacute infarction of rats.
Clinical studies using 18F-fluorodeoxyglucose suggest that this tracer may overestimate myocardial viability. This study aimed to elucidate whether 2-deoxyglucose accurately indicates myocardial viability at the early phase of myocardial infarction. Autoradiography with 14C-deoxyglucose was performed in fasting rats whose left coronary artery was occluded for 60 min and then reperfused. 14C-deoxyglucose was injected 30 min after the reperfusion (acute; n=10) or 1 week later (subacute; n=9). Infarction and risk areas were identified by triphenyl tetrazolium chloride or haematoxylin-eosin staining and methylene blue, respectively. Immuno-histochemical staining using anti-glucose transporter 1 and 4 antibodies was performed. At the acute stage, the uptake of deoxyglucose was consistent with the grade of anti-glucose transporter 4 expression. At the subacute stage, the uptake of deoxyglucose in poorly viable myocardium (543.4+/-343.7%: normalized with the uptake at the right ventricle) as well as in the viable one (335.2+/-149.8%) in the risk area was significantly greater than that in the remote area (116.4+/-94.9%, P<0.01). Anti-glucose transporter 1 was expressed in the poorly viable area where inflammatory cells infiltrated. It is concluded that deoxyglucose uptake by inflammatory cells which express anti-glucose transporter 1 causes overestimation of myocardial viability at subacute stage.
Hasegawa
S
S
Division of Tracer Kinetics, Department of Diagnositic Medicine, Osaka University Graduate School of Medicine, Suita, Japan. hasegawa@tracer.med.osaka-u.ac.jp
Kusuoka
H
H
Fukuchi
K
K
Yutani
K
K
Maruyama
K
K
Hori
M
M
Nishimura
T
T
D000208:Acute Disease; D000818:Animals; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D005189:False Positive Reactions; D007091:Image Processing, Computer-Assisted; D008297:Male; D018613:Microscopy, Confocal; D023421:Models, Animal; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology*; D015425:Myocardial Reperfusion / Q000379:methods; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D014018:Tissue Distribution; D014022:Tissue Survival
D002250:Carbon Radioisotopes; D003847:Deoxyglucose