Novel effect of vitamin K(1) (phylloquinone) and vitamin K(2) (menaquinone) on promoting nerve growth factor-mediated neurite outgrowth from PC12D cells.
The nerve growth factor (NGF)-potentiating effect of K vitamins on PC12D cells was investigated. Treatment of PC12D cells with vitamin K(1) or K(2) in the presence of NGF significantly enhanced the proportion of neurite-bearing cells and acetylcholinesterase activity compared with NGF treatment alone. The K vitamins-enhanced neurite outgrowth on PC12D cells was completely blocked by a protein kinase A (PKA) inhibitor or mitogen-activated protein kinase (MAPK) kinase inhibitor PD98059, whereas a protein kinase C inhibitor chelerythrine chloride did not significantly inhibit the enhancing effect of the K vitamins. These results suggest that the K vitamins enhance neurite outgrowth via the activation of PKA and MAPK-mediated signaling pathways in PC12D cells.
Tsang
Chi Kwan
CK
Marine and Highland Bioscience Center, Saga University, 152-1 Shonan-cho, Karatsu, 847-0021, Saga, Japan.
Kamei
Yuto
Y
D000818:Animals; D000933:Antifibrinolytic Agents / Q000378:metabolism / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D006128:Growth / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism*; D016501:Neurites / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured; D010837:Vitamin K 1 / Q000378:metabolism / Q000494:pharmacology*; D024482:Vitamin K 2 / Q000378:metabolism / Q000494:pharmacology*
D000933:Antifibrinolytic Agents; D004791:Enzyme Inhibitors; D005419:Flavonoids; D024482:Vitamin K 2; D010837:Vitamin K 1; D020932:Nerve Growth Factor; D017868:Cyclic AMP-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one