Effect of strand orientation on the interaction of berenil with DNA triple helices.
Using circular dichroism spectroscopy the ability of berenil, a minor groove binding drug, to induce triple helix formation was investigated with two oligonucleotides designed to form two intramolecular triplexes containing T*A:T and G*G:C triplets, which differ only by the orientation of their third strand: 5'-d(G4A4G4-[T4]-C4T4C4-[T4]-G4T4G4), and 5'-d(G4T4G4-[T4]-G4A4G4-[T4]-C4T4C4), where [T4] represents a stretch of four thymine residues. We demonstrate that when added to the duplex form of these oligonucleotides, berenil induces triplex structure formation only if the orientation of third strand is anti-parallel to the purine strand.
Durand
Maurice
M
Centre de Biophysique Moléculaire, Laboratoire conventionné avec l'Université d'Orléans et affilié à l'INSERM, France. durand@cnrs-orleans.fr
Seche
Edouard
E
Maurizot
Jean Claude
JC
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004133:Diminazene / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D014466:Ultraviolet Rays
D009841:Oligonucleotides; C051802:triplex DNA; D011189:Potassium Chloride; D004247:DNA; C003915:diminazene aceturate; D004133:Diminazene