title: Identification and characterization of a functionally distinct form of human estrogen receptor beta.
abstract: Estrogen receptors are important for the development and maintenance of many different tissues in the body including the breast, uterus, brain and bone. There are two known genes encoding estrogen receptors, Estrogen Receptor alpha (ER alpha) and Estrogen Receptor beta (ER beta). These receptors are transcription factors with distinct functional domains involved in DNA binding, ligand binding and transcriptional regulation. A novel isoform of human ER beta (ER beta 548) which includes an extended amino terminal domain has been identified. Isoform specific antibodies confirm the presence of this receptor in human tissue. Transactivation analysis with different estrogenic ligands indicates that ER beta 548 is functionally distinct from previously reported forms of ER beta.
authors.affiliation: Department of Atherosclerosis and Endocrinology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
authors.forename: Hilary A
authors.initials: HA
authors.lastname: Wilkinson
authors.forename: Johanna
authors.initials: J
authors.lastname: Dahllund
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Joel
authors.initials: J
authors.lastname: Yudkovitz
authors.forename: Sheng-Jian
authors.initials: SJ
authors.lastname: Cai
authors.forename: Stefan
authors.initials: S
authors.lastname: Nilsson
authors.forename: James M
authors.initials: JM
authors.lastname: Schaeffer
authors.forename: Sudha W
authors.initials: SW
authors.lastname: Mitra
mesh_terms: D015153:Blotting, Western; D003062:Codon / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D047629:Estrogen Receptor beta; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007536:Isomerism; D008297:Male; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D003062:Codon; D017931:DNA Primers; D018076:DNA, Complementary; D047629:Estrogen Receptor beta; D011960:Receptors, Estrogen