Presence of heat shock protein in chronic periapical pathology of pulpal origin.
In order to establish whether tissues damaged by Chronic Periapical Pathology (CPP) of endodontic origin produced heat shock protein (HSP) capable of attracting reactive lymphocytes, paraffin sections of samples from the oral cavity of 10 patients with CPP were incubated with commercially available anti-HSP monoclonal antibodies. Antibodies were evidenced employing the alkaline phosphatase immunoenzymatic method (DAKO). HSP was found in the lamina propria infiltrated by lymphocytes, in six of the ten samples. These results suggest that HSP may be one of the lymphocyte recruiting factors in the damaged area and opens new possibilities for further research.
Sierra
L
L
Dept. of Endodontics, Faculty of Dentistry, University of Buenos Aires, Argentina.
Laguens
M
M
Maresca
B
B
Fernández Monjes
J F
JF
Mascaró
A
A
D000293:Adolescent; D000328:Adult; D002634:Chemotaxis, Leukocyte; D002908:Chronic Disease; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000276:immunology* / Q000378:metabolism*
D006360:Heat-Shock Proteins