[Cloning and sequencing of fatty acid binding proteins gene in chicken].
A pair of primers was designed according to the sequence of mammal fatty acid binding protein (FABP) gene, then PCR amplified to chicken genome. After the product of PCR was cloned and sequenced, homologous comparison was done among porcine heart fatty acid binding protein gene and porcine adipocyte fatty acid binding protein gene. The result showed that the sequence of chicken FABP gene had 68% and 75% homology with porcine H-FABP and A-FABP gene respectively, and had 75% homology with porcine AFABP on amino acid level. The result of Northern showed that the gene only expressed in fat tissues.
Wang
Qi-Gui
QG
National Laboratories for Agribiotechnology, China Agricultural University, Beijing 100094, China.
Li
Ning
N
Deng
Xue-Mei
XM
Gu
Zhi-Liang
ZL
Liu
Zhao-Liang
ZL
Li
Hui
H
Chen
Zhi-Qiang
ZQ
Lian
Zheng-Xing
ZX
Wu
Chang-Xin
CX
D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA / Q000379:methods; D017385:Sequence Homology; D013552:Swine
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins