Nuclear receptor and apoptosis initiator NGFI-B is a substrate for kinase ERK2.
NGFI-B is an inducible orphan nuclear receptor that initiates apoptosis. Growth factors such as EGF activate the MAP kinase ERK, whose activity may determine if a cell survives or undergoes apoptosis. EGF stimulation of cells leads to phosphorylation of threonine in NGFI-B. Thr-142 of NGFI-B is comprised in a consensus MAP kinase site and was identified as a preferred substrate for ERK2 (but not ERK1) in vitro. These results suggest that NGFI-B may be a molecular target for ERK2 signals and thereby a substrate for crosstalk between a growth factor survival pathway and an inducible regulator of apoptosis.
Slagsvold
Hege H
HH
Institute of Pharmacy, University of Oslo, P.O. Box 1068 Blindern, N-0316 Oslo, Norway.
Østvold
Anne-Carine
AC
Fallgren
Asa B
AB
Paulsen
Ragnhild E
RE
D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D010766:Phosphorylation; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism*
D004268:DNA-Binding Proteins; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D014157:Transcription Factors; D019950:Mitogen-Activated Protein Kinase 1