Platelet and leukocyte activation during aortoiliac angiography and angioplasty.
OBJECTIVE
to evaluate platelet and leukocyte activation during aortoiliac angiography and percutaneous transluminal angioplasty (PTA).


METHODS
an observational study of 14 patients with aortoiliac atherosclerotic disease, nine of whom underwent PTA. The proportion of fibrinogen-, and P-selectin positive platelets, P-selectin expression on platelets, intraplatelet cGMP and cAMP, CD18 positive granulocytes, CD18 expression on granulocytes, plasma (p)-neopterin, p-TNF alpha and p- interleukin-6 were repeatedly measured in arterial blood during angiography and in venous blood before and after.


RESULTS
compared to a previous venous sample, arterial intraplatelet cAMP was increased proximal to the atherosclerotic lesion before contrast infusion and PTA (median 18 [range: 14-22] vs 16 [15-21] pmol/10(9) platelets p<0.05), and intraplatelet cGMP was increased proximal to the lesion after contrast infusion and PTA (1.2 [0.8-3.9] vs 0.9 [0.6-2.5] pmol/10(9) platelets p<0.05). Four hours after angiography, both the proportion of P-selectin positive platelets (28[11-55]%) and platelet P-selectin expression (9[6-40]) had decreased (p<0.05), from arterial values distal to the lesion before contrast infusion and PTA (57 [24-78]% and 26 [10-83]). Granulocyte CD18 expression was lower during angiography than in a previous venous sample.


CONCLUSIONS
the results are compatible with platelet but not leukocyte activation during peripheral angiography.
Barani
J
J
Department of Vascular Diseases, University of Lund, University Hospital, S-205 02 Malmö, Sweden.
Gottsäter
A
A
Mattiasson
I
I
Lindblad
B
B
D000368:Aged; D000369:Aged, 80 and over; D000792:Angiography; D000800:Angioplasty, Balloon; D001011:Aorta / Q000187:drug effects* / Q000503:physiopathology*; D001027:Aortography; D001161:Arteriosclerosis / Q000503:physiopathology* / Q000628:therapy*; D003287:Contrast Media / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007083:Iliac Artery / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000503:physiopathology*; D007962:Leukocytes / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects* / Q000502:physiology*
D003287:Contrast Media