Heterologous movement protein strongly modifies the infection phenotype of cucumber mosaic virus.
A hybrid virus (CMVcymMP) constructed by replacing the movement protein (MP) of cucumber mosaic cucumovirus (CMV) with that of cymbidium ringspot tombusvirus (CymRSV) was viable and could efficiently spread both cell to cell and long distance in host plants. The hybrid virus was able to move cell to cell in the absence of functional CP, whereas CP-deficient CMV was restricted to single inoculated cells. In several Chenopodium and Nicotiana species, the symptom phenotype of the hybrid virus infection was clearly determined by the foreign MP gene. In Nicotiana debneyi and Nicotiana tabacum cv. Xanthi, the hybrid virus could move systemically, contrary to CymRSV.
Huppert
Emese
E
Agricultural Biotechnology Center, Environmental Biosafety Research Institute, H-2100 Gödöllö, Hungary.
Szilassy
Dénes
D
Salánki
Katalin
K
Divéki
Zoltán
Z
Balázs
Ervin
E
D002213:Capsid / Q000172:deficiency / Q000235:genetics; D027465:Chenopodium quinoa / Q000821:virology; D017799:Cucumovirus / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D017353:Gene Deletion; D010935:Plant Diseases / Q000821:virology*; D053830:Plant Viral Movement Proteins; D016865:Reassortant Viruses; D011995:Recombination, Genetic; D014026:Nicotiana / Q000821:virology; D017864:Tombusvirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins