Immune response to synthetic peptides of dengue prM protein.
The immunological activities of five synthetic peptides of the prM protein of dengue-2 (DEN-2) virus containing B cell epitopes were evaluated in BALB/c mice. Two peptides elicited neutralizing antibodies against all four DEN serotypes. Virus-specific proliferative responses were demonstrated in mice immunized with four of the five peptides, demonstrating the presence of T cell epitopes. Mice immunized with three of the five peptides conjugated with bovine albumin showed statistically significant levels (P<0.05) of protection when challenged with DEN-2 virus. These results could constitute the basis for the establishment of the role of DEN virus pre and M antigens in the development of anti-flaviviral vaccines.
Vázquez
Susana
S
Virology Department, "Pedro Kouri" Tropical Medicine Institute, Autopista Novia del Mediodia, km 6, P.O. Box Marianao 13, Havana, Cuba. svazquez@ipk.sld.cu
Guzmán
María Guadalupe
MG
Guillen
Gerardo
G
Chinea
Glay
G
Pérez
Ana Beatriz
AB
Pupo
Maritza
M
Rodriguez
Rosmary
R
Reyes
Osvaldo
O
Garay
Hilda Elisa
HE
Delgado
Iselys
I
García
Gissel
G
Alvarez
Mayling
M
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology; D003715:Dengue / Q000276:immunology / Q000517:prevention & control; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000494:pharmacology; D014764:Viral Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000494:pharmacology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines