Strategies to elucidate the mechanism of excessive theca cell androgen production in PCOS.
Polycystic ovary syndrome (PCOS) is characterized by increased ovarian androgen production. We have developed a strategy to identify differentially transcribed genes in PCOS theca cells. Using long-term cultures of normal and PCOS theca cells, we are characterizing the complete repertoire of differentially transcribed genes, the common transcription factor mediators, and the signal transduction cascade(s) that are abnormal in PCOS theca cells. The latter will be interrogated for mutations/genetic variants linked to PCOS.
Strauss
Jerome F
JF
Center for Research on Reproduction and Women's Health, University of Pennsylvania Medical Center, 1354 BRBII, 421 Curie Boulevard, Philadelphia, PA 19104, USA. jfs3@mail.med.upenn.edu
Wood
Jennifer R
JR
Christenson
Lane K
LK
McAllister
Jan M
JM
D000728:Androgens / Q000096:biosynthesis*; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D003199:Computer Systems; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D015398:Signal Transduction / Q000235:genetics; D013382:Subtraction Technique; D013799:Theca Cells / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology
D000728:Androgens; D002843:Chromatin; D014157:Transcription Factors