Enzymatic degradation of and bovine serum albumin release from starch-acetate films.
The effect of acetylation of potato starch on swelling, enzymatic degradation, and bovine serum albumin (BSA, molecular mass 68 kDa) release rate from polymer films was studied. Potato starch and potato starch acetates (SA), having a degree of substitution of 1.9 or 2.6, were investigated. Polymer films were incubated in phosphate buffer solution pH 7.4 in the absence and presence of enzymes (alpha-amylase, amyloglucosidase, esterase) or in human serum. The acetylation of potato starch decreased its swelling considerably. Increased acetylation of starch also considerably retarded its enzymatic degradation. Due to the decreased swelling and degradation of SA films, BSA was released much slower from SA films than from potato starch films, both in the presence and absence of enzymes.
Tuovinen
Laura M
LM
Department of Pharmaceutics, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. laura.tuovinen@uku.fi
Peltonen
Soili H
SH
Suortti
Tapani M
TM
Crowther
Nicholas J
NJ
Elomaa
Matti A
MA
Järvinen
Kristiina P
KP
D004337:Drug Carriers; D004950:Esterases; D005087:Glucan 1,4-alpha-Glucosidase; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013213:Starch / Q000031:analogs & derivatives* / Q000737:chemistry*; D000516:alpha-Amylases
D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D013213:Starch; C050766:starch acetate; D004950:Esterases; D000516:alpha-Amylases; D005087:Glucan 1,4-alpha-Glucosidase