Cellular localization and function of Fas ligand (CD95L) in tumors.
The localization of CD95L in different cell types within tumors has not been well defined, and its role in tumor growth is uncertain. In this study, CD95L expression and its contribution to tumor growth are evaluated using genetic polymorphisms and adoptive transfer in genetically deficient mice. CD95L was detected in tumors in vivo at levels up to 10(4)-fold higher than those in cell culture and predominantly in host tumor-infiltrating macrophages, but not in tumor cells. Adoptive transfer into genetically deficient mice revealed that host CD95-CD95L function did not alter tumor growth, demonstrating that CD95L alone does not affect tumor growth.
Kurooka
Masayuki
M
Vaccine Research Center, NIAID, NIH, Bethesda, Maryland 20892, USA.
Nuovo
Gerard J
GJ
Caligiuri
Michael A
MA
Nabel
Gary J
GJ
D000818:Animals; D053222:Fas Ligand Protein; D005260:Female; D005434:Flow Cytometry; D007108:Immune Tolerance; D015151:Immunoblotting; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000737:chemistry* / Q000276:immunology; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D012333:RNA, Messenger