title: [Cloning and sequencing of fatty acid binding proteins gene in chicken].
abstract: A pair of primers was designed according to the sequence of mammal fatty acid binding protein (FABP) gene, then PCR amplified to chicken genome. After the product of PCR was cloned and sequenced, homologous comparison was done among porcine heart fatty acid binding protein gene and porcine adipocyte fatty acid binding protein gene. The result showed that the sequence of chicken FABP gene had 68% and 75% homology with porcine H-FABP and A-FABP gene respectively, and had 75% homology with porcine AFABP on amino acid level. The result of Northern showed that the gene only expressed in fat tissues.
authors.affiliation: National Laboratories for Agribiotechnology, China Agricultural University, Beijing 100094, China.
authors.forename: Qi-Gui
authors.initials: QG
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Deng
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Gu
authors.forename: Zhao-Liang
authors.initials: ZL
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Zheng-Xing
authors.initials: ZX
authors.lastname: Lian
authors.forename: Chang-Xin
authors.initials: CX
authors.lastname: Wu
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D050556:Fatty Acid-Binding Proteins; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009363:Neoplasm Proteins; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA / Q000379:methods; D017385:Sequence Homology; D013552:Swine
chemical_list: D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins