Regions of H- and K-ras that provide organ specificity/potency in mammary cancer induction.
Organ-specific cancers with activated ras oncogenes most often are associated exclusively with only one ras isoform. For example, only H-ras activation is associated with rat mammary cancers. The mechanism underlying this specificity is mostly unknown. We have shown previously that this tissue specificity of Ras isoforms is defined by the Ras protein itself and not by differential gene expression among Ras family members. Here we show that elements in the known domains in the hypervariable region of Ras (amino acids 170-189) interact in part to control this mammary/H-Ras specificity. In addition, these in vivo mammary studies for the first time identify domains in the mostly homologous region of Ras (amino acids 1-169) that strongly influence the oncogenic potency/specificity of H-Ras.
Kim
Kwanghee
K
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Lindstrom
Mary J
MJ
Gould
Michael N
MN
D000595:Amino Acid Sequence; D000818:Animals; D011905:Genes, ras; D008325:Mammary Neoplasms, Experimental / Q000209:etiology* / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D051381:Rats; D011920:Rats, Inbred WF; D018631:ras Proteins / Q000737:chemistry
D018631:ras Proteins