Malignant transformation by the eukaryotic translation initiation factor 3 subunit p48 (eIF3e).
Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Stable expression of a truncated eIF3e in NIH 3T3 cells causes malignant transformation by four criteria: foci formation; anchorage independent growth; accelerated growth; and lack of contact inhibition. Stable expression of full-length eIF3e does not cause transformation. The truncated eIF3e also inhibits the onset of apoptosis caused by serum starvation.
Mayeur
Greg L
GL
Department of Biological Chemistry, School of Medicine, University of California, Davis, CA 95616, USA.
Hershey
John W B
JW
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D039621:Eukaryotic Initiation Factor-3; D017353:Gene Deletion; D051379:Mice; D010448:Peptide Initiation Factors / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*
D039621:Eukaryotic Initiation Factor-3; D010448:Peptide Initiation Factors; D011518:Proto-Oncogene Proteins