Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I real-time RT-PCR.
Vandesompele
Jo
J
Center for Medical Genetics, Ghent University Hospital, B-9000 Ghent, Belgium.
De Paepe
Anne
A
Speleman
Frank
F
D052160:Benzothiazoles; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D003959:Diamines; D019281:Dimerization; D005456:Fluorescent Dyes / Q000737:chemistry; D020869:Gene Expression Profiling; D009930:Organic Chemicals; D011804:Quinolines; D012313:RNA / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D052160:Benzothiazoles; D017931:DNA Primers; D018076:DNA, Complementary; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D012313:RNA; D003851:Deoxyribonucleases