Generation of infectious cDNA clones of a Korean strain of tomato aspermy virus.
Infectious full-length cDNA clones of the Korean strain of tomato aspermy cucumovirus (KC-TAV) were constructed using a long-template reverse transcription-polymerase chain reaction. The in vitro RNA transcripts, which were produced using T7 RNA polymerase from full-length cDNAs, could systemically infect the Nicotiana tabacum cv. Xanti-nc plants and induce systemic symptoms on the upper leaves that are similar to the wildtype KC-TAV. The complete nucleotide sequences of genomic RNAs of KC-TAV were determined from the infectious full-length cDNA clones. RNA1 and RNA2 of KC-TAV contain 3412 nucleotides and 3074 nucleotides, respectively. RNA3 of KC-TAV, 2222 nucleotides long, encodes the 3a protein and coat protein (CP) that are separated by 295 nucleotides intergenic region. The overall sequence analysis of the whole genome of KC-TAV revealed a strong homology (99%) to the genome of the V-TAV strain, the only strain whose entire genomic nucleotide sequence was available in the database, and an overall 60% homology to those of other cucumber mosaic virus and peanut stunt virus strains. A sequence comparison analysis of the deduced amino acid sequences of cDNAs of KC-TAV RNA 1, 2, and 3 indicates that there is no genetic diversity in the TAV population, although the virus exists in different geographical distributions.
Choi
Seung Kook
SK
Graduate School of Biotechnology, Korea University, Seoul.
Choi
Jang Kyung
JK
Ryu
Ki Hyun
KH
Park
Won Mok
WM
D000595:Amino Acid Sequence; D001483:Base Sequence; D011723:Chrysanthemum cinerariifolium / Q000821:virology; D003001:Cloning, Molecular; D017799:Cucumovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D007723:Korea; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral