title: Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata.
abstract: Phytochemical characteristics and antioxidant activities of the crude and fractionated plant extracts of Anoectochilus formosanus were evaluated using five different assay systems. An acid-treatment (2 N HCl in 95% ethanol) was employed to treat a butanol fraction (BuOH), creating an acid-hydrolyzed BuOH fraction. The IC(50) values for DPPH radicals in the BuOH and acid-hydrolyzed BuOH fractions were 0.521 and 0.021 mg/mL, respectively. The acid-hydrolyzed BuOH exhibited approximately 5-fold higher activity in scavenging superoxide anion than catechin. The acid-hydrolyzed BuOH fraction also effectively protected phi x174 supercoiled DNA against strand cleavage induced by H(2)O(2) and reduced oxidative stress in HL-60 cells. Metabolite profiling showed that the aglycones of flavonoid glycosides in BuOH were produced after acid hydrolytic treatment, and this resulted in a significant increase in antioxidant activities of acid-hydrolyzed BuOH. One new diarylpentanoid, kinsenone, and three known flavonoid glycosides and their derivatives were identified for the first time from A. formosanus, with strong antioxidant properties.
authors.affiliation: Institute of BioAgricultural Sciences, Academia Sinica, Taipei 115, Taiwan, R.O.C.
authors.forename: Sheng-Yang
authors.initials: SY
authors.lastname: Wang
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.forename: Hsing-Ning
authors.initials: HN
authors.lastname: Chang
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Kang
authors.forename: Hsin-Sheng
authors.initials: HS
authors.lastname: Tsay
authors.forename: Ku-Feng
authors.initials: KF
authors.lastname: Lin
authors.forename: Ning-Sun
authors.initials: NS
authors.lastname: Yang
authors.forename: Lie-Fen
authors.initials: LF
authors.lastname: Shyur
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D010584:Bacteriophage phi X 174 / Q000235:genetics; D000440:Butanols; D002392:Catechin / Q000494:pharmacology; D004278:DNA, Superhelical / Q000187:drug effects; D004279:DNA, Viral / Q000187:drug effects; D016166:Free Radical Scavengers; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D029595:Orchidaceae / Q000737:chemistry*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D013481:Superoxides
chemical_list: D000975:Antioxidants; D000440:Butanols; D004278:DNA, Superhelical; D004279:DNA, Viral; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D002392:Catechin; D006861:Hydrogen Peroxide