Cooperia punctata trickle infections: parasitological parameters and evaluation of a Cooperia recombinant 14.2 kDa protein ELISA for estimating cumulative exposure of calves.
Three groups of four calves each were trickle infected with three different levels of Cooperia punctata: 310 (group A), 1250 (group B) and 5000 (group C) third stage infective larvae (L3) twice a week over a 17-week period. Group D was the non-infected control group. Parasitological parameters as faecal egg counts (epg), worm burdens, size of worms and number of eggs per female were collected and the differences between the groups compared. Serological analyses were also conducted to investigate the efficiency of a recombinant C. oncophora CoES 14.2kDa protein in an ELISA to detect C. punctata antibodies. Group C had higher faecal egg counts until week 9 when the values decreased to those in group B. Mean faecal egg counts in group A were always lower than in the two other infected groups. The worm burdens were highest in group C, and lowest in group A, although the number of worms as a percentage of total larval intake was higher for the lower group. The mean length of the worms was shorter and the number of eggs per female lower for group C than for both other groups. ELISA using the CoES 14.2kDa proved to be efficient in measuring C. punctata antibodies. For group C it took 4 weeks to get increased levels of antibodies and this was one and 2 months more for groups B and A, respectively. Overall, there was a congruent relation between C. punctata antibodies and the cumulative exposure to the three different levels of trickle infections.
Yatsuda
A P
AP
Department of Infectious Diseases and Immunology, Division of Parasitology and Tropical Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands. a.yatsuda@vet.uu.nl
Kooyman
F N J
FN
Ploeger
H W
HW
Vieira-Bressan
M C R
MC
de Vries
E
E
Eysker
M
M
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000096:biosynthesis; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary*; D007814:Larva; D008970:Molecular Weight; D010270:Parasite Egg Count / Q000662:veterinary; D011897:Random Allocation; D011994:Recombinant Proteins / Q000276:immunology; D014251:Trichostrongyloidea / Q000254:growth & development*; D014252:Trichostrongyloidiasis / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins