title: Microglia promote glioma migration.
abstract: Diffuse astrocytic gliomas extensively infiltrate brain tissue and contain numerous microglial cells, but it is unknown whether these two characteristic features are pathogenetically related. We therefore studied the effects of murine microglial cells on motility of GL261 mouse glioma cells using Boyden chamber assays. In the presence of microglia, glioma cell migration occurred earlier, and after 48 h it was threefold higher as compared to incubations without microglia. This effect was mediated by substances released from microglia, because similar effects were observed by microglia-conditioned medium, and it was specific to microglia, because oligodendroglia and endothelial cells only weakly stimulated glioma cell migration. Microglia activating substances (GM-CSF, LPS) led to a further increase of motility. These data support the notion that microglia accumulation in diffuse glial tumors does not merely represent a nonspecific reaction to tissue injury, but reflects participation of these cells in supporting and promoting the invasive phenotype of astrocytoma cells.
authors.affiliation: Institute of Neuropathology, University Hospital Münster, Domagkstr. 19, 48129 Münster, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Bettinger
authors.forename: Solon
authors.initials: S
authors.lastname: Thanos
authors.forename: Werner
authors.initials: W
authors.lastname: Paulus
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005910:Glioma / Q000473:pathology* / Q000503:physiopathology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000473:pathology / Q000502:physiology*; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology
chemical_list: D017077:Culture Media, Conditioned; D016178:Granulocyte-Macrophage Colony-Stimulating Factor