title: Quantification of methyldopa in human plasma by high-performance liquid chromatography-electrospray tandem mass spectrometry application to a bioequivalence study.
abstract: A method based on LC-MS-MS is described for the determination of methyldopa in human plasma using dopa-phenyl-D3 as the internal standard. The method has a chromatographic run time of 5.5 min and was linear in the range of 20-5000 ng/ml. The limit of quantitation was 20 ng/ml, the intra-day precisions were 7.3, 5.4 and 4.3% and the intra-day accuracies were -8.0, -1.3 and -2.0% for 30, 600 and 3000 ng/ml, respectively. The inter-day precisions were 7.7, 0.5 and 0.7% and the inter-day accuracies were 0.2, -1.1 and -2.3%, respectively, for the above concentrations. This method was employed in a bioequivalence study of two tablet formulations of methyldopa.
authors.affiliation: Department of Pharmacology, State University of Campinas-UNICAMP, Campinas, Brazil.
authors.forename: Celso H
authors.initials: CH
authors.lastname: Oliveira
authors.forename: Rafael E
authors.initials: RE
authors.lastname: Barrientos-Astigarraga
authors.forename: Mauro
authors.initials: M
authors.lastname: Sucupira
authors.forename: Gustavo S
authors.initials: GS
authors.lastname: Graudenz
authors.forename: Marcelo N
authors.initials: MN
authors.lastname: Muscará
authors.forename: Nucci Gilberto
authors.initials: NG
authors.lastname: De
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008750:Methyldopa / Q000097:blood* / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013810:Therapeutic Equivalency
chemical_list: D008750:Methyldopa