Delivery of protein antigens and DNA by attenuated intracellular bacteria.
On the basis of attenuated intracellular bacteria, we have developed two delivery systems for either heterologous proteins or DNA vaccine vectors. The first system utilizes attenuated strains of Gram-negative bacteria which are engineered to secrete heterologous antigens via the alpha-hemolysin secretion system (type I) of Escherichia coli. The second system is based on attenuated suicide strains of Listeria monocytogenes, which are used for the direct delivery of eukaryotic antigen expression vectors into professional antigen-presenting cells (APC) like macrophages and dendritic cells in vitro and can be also used in animal models.
Gentschev
Ivaylo
I
Department of Microbiology, University of Wuerzburg, Germany. gentsch@biozentrum.uni-wuerzburg.de
Dietrich
Guido
G
Spreng
Simone
S
Pilgrim
Sabine
S
Stritzker
Jochen
J
Kolb-Mäurer
Annette
A
Goebel
Werner
W
D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005057:Eukaryotic Cells / Q000276:immunology; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000276:immunology; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008089:Listeria monocytogenes / Q000276:immunology; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000235:genetics; D014614:Vaccines, Synthetic
D000942:Antigens, Bacterial; D006460:Hemolysin Proteins; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic