title: [Involvement of FAK, PI3-K and PKC in cell adhesion induced by microtubule disruption].
abstract: We have previously shown that microtubule disruption results in an increase in cell adhesion to ECM proteins. In this work we show that this enhanced cell attachment was completely abolished by specific inhibitors of tyrosine-kinases, PI3-K and PKCs. Microtubule depolymerisation was associated with an important increased in tyrosine phosphorylation of FAK and paxilline, as well as with subcellular localisation of PKCgamma, delta and epsilon. We also observed significant alterations in actin cytoskeleton leading to reduced cell spreading. Thus, microtubule depolymerisation appears to activate various intracellular kinases that lead to actin cytoskeletal changes and to an increase of integrin-dependent adhesion. Whether this enhanced attachment is due to intracellular events resulting in changes in integrin affinity or avidity remains to be determined.
authors.affiliation: Laboratoire de biochimie cellulaire, CNRS UMR 6032, Faculté de Pharmacie, 27, bd Jean-Moulin, 13385 Marseille Cedex 5.
authors.forename: Azzeddine
authors.initials: A
authors.lastname: Kadi
authors.forename: Virginie
authors.initials: V
authors.lastname: Berthet
authors.forename: Véronique
authors.initials: V
authors.lastname: Pichard
authors.forename: Brigitte
authors.initials: B
authors.lastname: Abadie
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Rognoni
authors.forename: Jacques
authors.initials: J
authors.lastname: Marvaldi
authors.forename: José
authors.initials: J
authors.lastname: Luis
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D051418:Focal Adhesion Kinase 2; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D011493:Protein Kinase C / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077235:Vinorelbine
chemical_list: D000970:Antineoplastic Agents; D014747:Vinblastine; D019869:Phosphatidylinositol 3-Kinases; D011505:Protein-Tyrosine Kinases; D051418:Focal Adhesion Kinase 2; C103547:PTK2B protein, human; D011493:Protein Kinase C; D017239:Paclitaxel; D000077235:Vinorelbine; D015739:Nocodazole