Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata.
Phytochemical characteristics and antioxidant activities of the crude and fractionated plant extracts of Anoectochilus formosanus were evaluated using five different assay systems. An acid-treatment (2 N HCl in 95% ethanol) was employed to treat a butanol fraction (BuOH), creating an acid-hydrolyzed BuOH fraction. The IC(50) values for DPPH radicals in the BuOH and acid-hydrolyzed BuOH fractions were 0.521 and 0.021 mg/mL, respectively. The acid-hydrolyzed BuOH exhibited approximately 5-fold higher activity in scavenging superoxide anion than catechin. The acid-hydrolyzed BuOH fraction also effectively protected phi x174 supercoiled DNA against strand cleavage induced by H(2)O(2) and reduced oxidative stress in HL-60 cells. Metabolite profiling showed that the aglycones of flavonoid glycosides in BuOH were produced after acid hydrolytic treatment, and this resulted in a significant increase in antioxidant activities of acid-hydrolyzed BuOH. One new diarylpentanoid, kinsenone, and three known flavonoid glycosides and their derivatives were identified for the first time from A. formosanus, with strong antioxidant properties.
Wang
Sheng-Yang
SY
Institute of BioAgricultural Sciences, Academia Sinica, Taipei 115, Taiwan, R.O.C.
Kuo
Yueh-Hsiung
YH
Chang
Hsing-Ning
HN
Kang
Pei-Ling
PL
Tsay
Hsin-Sheng
HS
Lin
Ku-Feng
KF
Yang
Ning-Sun
NS
Shyur
Lie-Fen
LF
D000975:Antioxidants / Q000032:analysis*; D010584:Bacteriophage phi X 174 / Q000235:genetics; D000440:Butanols; D002392:Catechin / Q000494:pharmacology; D004278:DNA, Superhelical / Q000187:drug effects; D004279:DNA, Viral / Q000187:drug effects; D016166:Free Radical Scavengers; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D029595:Orchidaceae / Q000737:chemistry*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D013481:Superoxides
D000975:Antioxidants; D000440:Butanols; D004278:DNA, Superhelical; D004279:DNA, Viral; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D017665:Hydroxyl Radical; D002392:Catechin; D006861:Hydrogen Peroxide