title: In vivo tissue-specific regulation of the human papillomavirus type 18 early promoter by estrogen, progesterone, and their antagonists.
abstract: Human papillomavirus type 18 is a causative agent of epithelial cancers in the uterine cervix. We show here that estrogen and progesterone activate beta-galactosidase expression from the early promoter of this virus in the genital epithelia of transgenic mice. Ovariectomy caused suppression of transgene expression exclusively in vagina and cervix epithelia. Beta-galactosidase expression could be restored in ovariectomized females by administration of estrogen, alone or in combination with progesterone. Further, rescue of transgene expression was inhibited by the estrogen antagonist tamoxifen and the anti-progesterone RU486, suggesting that this was a specific effect.
authors.affiliation: Institute of Biomedical Research, National Autonomous University of Mexico (UNAM), 04510 Mexico City, Mexico.
authors.forename: Néstor
authors.initials: N
authors.lastname: Morales-Peza
authors.forename: Prasert
authors.initials: P
authors.lastname: Auewarakul
authors.forename: Victoria
authors.initials: V
authors.lastname: Juárez
authors.forename: Alejandro
authors.initials: A
authors.lastname: García-Carrancá
authors.forename: Angel
authors.initials: A
authors.lastname: Cid-Arregui
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D030762:Estrous Cycle; D005260:Female; D015967:Gene Expression Regulation, Viral; D006727:Hormone Antagonists / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015735:Mifepristone / Q000494:pharmacology; D010052:Ovariectomy; D027383:Papillomaviridae / Q000187:drug effects / Q000235:genetics*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D013629:Tamoxifen / Q000494:pharmacology; D019076:Transgenes; D014621:Vagina / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D006727:Hormone Antagonists; D014764:Viral Proteins; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D001616:beta-Galactosidase