Effect of antiallergic drugs on interleukin 5-induced eosinophil infiltration of rat airways.
Interleukin (IL)-5 is thought to play important roles in asthma and to be a potential therapeutic target. An intratracheal injection of murine recombinant IL-5 (3-30 microg/animal) induced a dose-dependent increase in the number of eosinophils in the bronchoalveolar lavage fluid of Brown Norway (BN) rats 24 h after administration. Bovine serum albumin (30pg/animal), used as reference material, did not cause any change. The reaction was not observed in F344 rats. The increase in the number of eosinophils did not accompany bronchial hyperreactivity in BN or F344 rats. Prednisolone (3-10 mg/kg, i.p.) and emedastine (30 mg/kg, p.o.) reduced the increased number of eosinophils induced by the IL-5 challenge. These results suggest that IL-5 is a potent inducer of eosinophils in the airway of BN rats. Prednisolone and emedastine are effective against IL-5-induced eosinophilia.
Takizawa
Toshiaki
T
Department of Pharmacology, Gifu Pharmaceutical University, Japan. ttakizaw@kowa.co.jp
Kawada
Naoki
N
Tanaka
Hiroyuki
H
Nagai
Hiroichi
H
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology; D001980:Bronchi / Q000473:pathology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015848:Interleukin-5 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D011239:Prednisolone / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000494:pharmacology
D018926:Anti-Allergic Agents; D001562:Benzimidazoles; D015848:Interleukin-5; D011994:Recombinant Proteins; C043345:emedastine; D011239:Prednisolone