title: Elimination of primer-dimer artifacts and genomic coamplification using a two-step SYBR green I real-time RT-PCR.
authors.affiliation: Center for Medical Genetics, Ghent University Hospital, B-9000 Ghent, Belgium.
authors.forename: Jo
authors.initials: J
authors.lastname: Vandesompele
authors.forename: Anne
authors.initials: A
authors.lastname: De Paepe
authors.forename: Frank
authors.initials: F
authors.lastname: Speleman
mesh_terms: D052160:Benzothiazoles; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D003959:Diamines; D019281:Dimerization; D005456:Fluorescent Dyes / Q000737:chemistry; D020869:Gene Expression Profiling; D009930:Organic Chemicals; D011804:Quinolines; D012313:RNA / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D052160:Benzothiazoles; D017931:DNA Primers; D018076:DNA, Complementary; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D012313:RNA; D003851:Deoxyribonucleases