title: Vesicular stomatitis virus glycoprotein does not determine the site of virus release in polarized epithelial cells.
abstract: In polarized epithelial cells, the vesicular stomatitis virus glycoprotein is segregated to the basolateral plasma membrane, where budding of the virus takes place. We have generated recombinant viruses expressing mutant glycoproteins without the basolateral-membrane-targeting signal in the cytoplasmic domain. Though about 50% of the mutant glycoproteins were found at the apical plasma membranes of infected MDCK cells, the virus was still predominantly released at the basolateral membranes, indicating that factors other than the glycoprotein determine the site of virus budding.
authors.affiliation: Institut für Virologie, Tierärztliche Hochschule, Hannover, Germany. Gert.Zimmer@tiho-hannover.de
authors.forename: Gert
authors.initials: G
authors.lastname: Zimmer
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Zimmer
authors.forename: Ina
authors.initials: I
authors.lastname: Trotz
authors.forename: Georg
authors.initials: G
authors.lastname: Herrler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000821:virology*; D007668:Kidney; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000502:physiology*; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins