title: D-amino-acid oxidase--an improved production of the enzyme by the yeast Trigonopsis variabilis in a laboratory fermentor.
abstract: The cultivation of the yeast Trigonopsis variabilis producing D-amino-acid oxidase (an enzyme participating in the transformation of cephalosporin C into 7-aminocephalosporanic acid for the production of beta-lactam antibiotics) was controlled by changes of dissolved oxygen tension and extended fermentation times. The production technology was optimized on a laboratory scale and scale-up parameters were identified.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
authors.forename: P
authors.initials: P
authors.lastname: Kujan
authors.forename: A
authors.initials: A
authors.lastname: Prell
authors.forename: H
authors.initials: H
authors.lastname: Safár
authors.forename: P
authors.initials: P
authors.lastname: Holler
authors.forename: K
authors.initials: K
authors.lastname: Plhácková
authors.forename: M
authors.initials: M
authors.lastname: Sobotka
mesh_terms: D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D003605:D-Amino-Acid Oxidase / Q000096:biosynthesis*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
chemical_list: D003470:Culture Media; D003605:D-Amino-Acid Oxidase; D010100:Oxygen