title: Characterization of toxin from cheilanthes fern and its effect on lymphocyte proliferation and DNA fragmentation.
abstract: Thin layer chromatography of aqueous extract of whole Cheilanthesfarinosa fern indicated the presence of ptaquiloside or ptaquiloside like compound, coinciding Rf values with that of Pterosin B standard. HPLC analysis revealed the presence of 26.3 mg/kg ptaquiloside. In vitro studies of the aqueous extract on lymphocyte culture revealed a correlation between stimulative indices and concentration of aqueous extract. Stimulation in lymphocyte proliferation was in order of bracken > cheilanthes > ConA> ptaquiloside standard. On incubation of lymphocyte with aqueous extract of ferns, no DNA damage was observed in isolated DNA.
authors.affiliation: Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar.
authors.forename: K A
authors.initials: KA
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Kataria
authors.forename: R
authors.initials: R
authors.lastname: Somvanshi
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Saini
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D053938:DNA Fragmentation / Q000187:drug effects*; D029624:Ferns / Q000737:chemistry*; D005562:Formazans / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D010936:Plant Extracts / Q000633:toxicity*; D010947:Plants, Toxic / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D005562:Formazans; D010936:Plant Extracts; D013778:Tetrazolium Salts; C079782:MTT formazan