title: The role of eicosanoids on Rhodnius heme-binding protein (RHBP) endocytosis by Rhodnius prolixus ovaries.
abstract: The participation of eicosanoids and second messengers on the regulation of RHBP endocytosis by the ovaries was investigated, using [(125)I]RHBP in experiments in vivo and in vitro. Addition of PGE(2) (one of the products of the cyclooxygenase pathway) decreased in vitro the uptake of RHBP by 35%. The rate of RHBP endocytosis increased in the presence of indomethacin, a potent cyclooxigenase inhibitor, up to 50% in vitro and up to 55% in vivo, thus giving support to the role of cyclooxygenase derivatives on endocytosis regulation. The amount of PGE(2) secreted to the culture medium by the cells of Rhodnius prolixus ovaries was 1.1 ng/ovary following RHBP uptake assay. The amount of PGE(2) decreases approximately 25% in the presence of 5 microM indomethacin. Using a scanning electron microscope we have observed that neither the surface area nor the patencies of follicle cells were affected by treatment with indomethacin, thus suggesting that, its effect is elicited in the oocyte. Finally, we have identified two ovarian peptides that were dephosphorylated after the indomethacin treatment (18 and 25 kDa). Taken together these data show that local mediators such as eicosanoids act upon the oocytes controlling RHBP endocytosis, perhaps using the protein phosphorylation signal transduction pathway.
authors.affiliation: Laboratório de Entomologia Médica do Programa de Parasitologia e Biologia Celular, IBCCF, CCS, UFRJ, Cidade Universitária, Ilha do Fundão, 21,941-590, Rio de Janeiro, Brazil.
authors.forename: Marcelo N
authors.initials: MN
authors.lastname: Medeiros
authors.forename: Danielle M P
authors.initials: DM
authors.lastname: Oliveira
authors.forename: Gabriela O
authors.initials: GO
authors.lastname: Paiva-Silva
authors.forename: Mário A C
authors.initials: MA
authors.lastname: Silva-Neto
authors.forename: Alexandre
authors.initials: A
authors.lastname: Romeiro
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bozza
authors.forename: Hatisaburo
authors.initials: H
authors.lastname: Masuda
authors.forename: Ednildo A
authors.initials: EA
authors.lastname: Machado
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D015777:Eicosanoids / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D005260:Female; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007457:Iodine Radioisotopes; D009924:Organ Culture Techniques; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D012239:Rhodnius / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D016861:Cyclooxygenase Inhibitors; D015777:Eicosanoids; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D007457:Iodine Radioisotopes; D015232:Dinoprostone; D007213:Indomethacin