title: Purification, crystallization and preliminary X-ray diffraction studies of a Ca2+-binding protein, human S100P.
abstract: S100P, a Ca(2+)-binding protein, is a member of the S100 family. Its presence is associated with the development of prostate cancer, but its cellular function is not known. Recombinant human S100P has been expressed and purified in bacterial cells and crystals of human S100P in the calcium-bound state have been grown using the vapour-diffusion technique with PEG 4000 as precipitant. Diffraction data have been obtained to a resolution of 2.0 A from a single frozen S100P crystal which belongs to the space group P4(1)2(1)2, with unit-cell parameters a = b = 60.8, c = 47.6 A.
authors.affiliation: Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Life Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Guozhen
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Roger
authors.initials: R
authors.lastname: Barraclough
authors.forename: Philip S
authors.initials: PS
authors.lastname: Rudland
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D006801:Humans; D009363:Neoplasm Proteins; D014961:X-Ray Diffraction
chemical_list: D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; C103003:S100P protein, human