title: Endothelial nitric oxide synthase gene polymorphisms in Behçet's disease.
abstract: OBJECTIVE
  To analyze potential associations of Glu-Asp298 polymorphism in exon 7 and 4 a/b polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) gene with susceptibility for Behçet's disease (BD).
  
  
  METHODS
  Seventy-three consecutive Italian patients who satisfied the International Study Group criteria for BD and 135 healthy blood donor controls from the same geographic area were genotyped by polymerase chain reaction and allele-specific oligonucleotide techniques for eNOS polymorphisms in exon 7 and in intron 4.
  
  
  RESULTS
  The distribution of the Glu-Asp298 genotype differed significantly between BD patients and controls (p(corr) = 0.00009). Allele Asp298 was significantly more frequent in BD patients than in controls (p(corr) = 0.0006, OR 2.1, 95% CI 1.5-3.3). The distribution of 4 a/b genotype was similar in patients and controls.
  
  
  CONCLUSIONS
  Our findings show that Glu-Asp298 polymorphism of eNOS gene is associated with BD susceptibility.
authors.affiliation: Laboratorio di Biologia Molecolare, Ospedale di Reggio Emilia, Italy. salvarani.carlo@asmn.re.it
authors.forename: Carlo
authors.initials: C
authors.lastname: Salvarani
authors.forename: Luigi
authors.initials: L
authors.lastname: Boiardi
authors.forename: Bruno
authors.initials: B
authors.lastname: Casali
authors.forename: Ignazio
authors.initials: I
authors.lastname: Olivieri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ciancio
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Cantini
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Salvi
authors.forename: Renato
authors.initials: R
authors.lastname: Malatesta
authors.forename: Marcello
authors.initials: M
authors.lastname: Govoni
authors.forename: Francesco
authors.initials: F
authors.lastname: Trotta
authors.forename: Davide
authors.initials: D
authors.lastname: Filippini
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Paolazzi
authors.forename: Davide
authors.initials: D
authors.lastname: Nicoli
authors.forename: Enrico
authors.initials: E
authors.lastname: Farnetti
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Macchioni
mesh_terms: D000328:Adult; D001528:Behcet Syndrome / Q000201:enzymology / Q000235:genetics*; D004730:Endothelium, Vascular / Q000201:enzymology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic
chemical_list: D009569:Nitric Oxide; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III