Mannose receptor-mediated regulation of serum glycoprotein homeostasis.
Carbohydrates are thought to function as tags that mark circulatory glycoproteins for rapid clearance. To examine the role of the mannose receptor (MR) in glycoprotein clearance, we generated mice genetically deficient in MR. MR-/- mice were defective in clearing proteins bearing accessible mannose and N-acetylglucosamine residues and had elevated levels of eight different lysosomal hydrolases. Proteomic analysis of MR-/- and control mouse sera showed that an additional 4 out of 52 proteins identified were elevated in MR-/- serum. Each of these is up-regulated during inflammation and wound healing. Thus, MR appears to operate as an essential regulator of serum glycoprotein homeostasis.
Lee
Sena J
SJ
Laboratory of Molecular Immunology, The Rockefeller University, 1230 York Avenue, Box #220, New York, NY 10021, USA.
Evers
Stefan
S
Roeder
Daniel
D
Parlow
Albert F
AF
Risteli
Juha
J
Risteli
Leila
L
Lee
Y C
YC
Feizi
Ten
T
Langen
Hanno
H
Nussenzweig
Michel C
MC
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005966:Glucuronidase / Q000097:blood / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry; D006207:Half-Life; D006706:Homeostasis; D006867:Hydrolases / Q000097:blood / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D037181:Lectins, C-Type; D008099:Liver / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008358:Mannose / Q000032:analysis / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D013058:Mass Spectrometry; D051379:Mice; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D015854:Up-Regulation
D001798:Blood Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D006867:Hydrolases; D005966:Glucuronidase; D008358:Mannose