"Omic" approaches for unraveling signaling networks.
Signaling pathways are crucial for cell differentiation and response to cellular environments. Recently, a large number of approaches for the global analysis of genes and proteins have been described. These have provided important new insights into the components of different pathways and the molecular and cellular responses of these pathways. This review covers genomic and proteomic (collectively referred to as "omic") approaches for the global analysis of cell signaling, including gene expression profiling and analysis, protein-protein interaction methods, protein microarrays, mass spectroscopy and gene-disruption and engineering approaches.
Zhu
Heng
H
Department of Molecular, Cellular and Developmental Biology, Yale University, 266 Whitney Avenue, New Haven, CT 06520, USA.
Snyder
Michael
M
D000818:Animals; D029721:Drosophila Proteins; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000032:analysis / Q000378:metabolism; D020543:Proteome; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D020798:Two-Hybrid System Techniques
D029721:Drosophila Proteins; D009124:Muscle Proteins; D020543:Proteome; C109229:ko protein, Drosophila; D011494:Protein Kinases