Analysis of factors associated with low peripheral blood progenitor cell collection in normal donors.
BACKGROUND
Predictive factors of the response to rHuG-CSF in normal donors have not been extensively studied.


METHODS
We analyzed factors influencing CD34+ cell yield in the 1st day of collection in 261 healthy donors from the Spanish National Donor Registry. The median age was 38 years (range, 2-72). The median dose of rHuG-CSF was 10 microg per kg per day (range, 5-20) over 4 days. In 103 donors (40%), <4 x 10(6) per kg CD34+ cells were collected. The variables that were analyzed included age, sex, weight, basal complete blood cell count, dose, type of rHuGCSF and schedule of administration, and maximum WBC count before apheresis.


RESULTS
By univariate analysis, the maximum WBC count (<50 vs. >or=50 x 10(9)/L, p = 0.004), advanced age (p = 0.008), and number of daily rHuG-CSF doses (one vs. two; p = 0.01) correlated with the number of CD34+ cells collected. By multivariate analysis, donors age (<38 vs. >or=38 years; p = 0.014) and a single daily dose of rHuG-CSF (p = 0.005) were the two variables that significantly predicted a low CD34+ cell yield.


CONCLUSIONS
Donors' age, with a threshold of 38 years or more, and the rHuG-CSF schedule are the factors that significantly affected CD34+ cell mobilization and collection in healthy donors.
de la Rubia
Javier
J
Hematology Service, Hospital La Fe, Valencia, Spain. delarubia_jav@gva.es
Arbona
Cristina
C
de Arriba
Felipe
F
del Cañizo
Consuelo
C
Brunet
Salut
S
Zamora
Concha
C
Díaz
Miguel A
MA
Bargay
Joan
J
Petit
José
J
de la Serna
Javier
J
Insunza
Andrés
A
Arrieta
Rosario
R
Pascual
María J
MJ
Serrano
David
D
Sanjuan
Isabel
I
Espigado
Ildefonso
I
Alegre
Adrián
A
Martínez
Dobleta
D
Verdeguer
Amparo
A
Martínez
Carmen
C
Benlloch
Luis
L
Sanz
Miguel A
MA
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D001772:Blood Cell Count; D001835:Body Weight; D001853:Bone Marrow / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011994:Recombinant Proteins; D012737:Sex Factors
D018952:Antigens, CD34; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim