title: Rapid monitoring of recombinant protein products: a comparison of current technologies.
abstract: Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.
authors.affiliation: Research School of Biosciences, University of Kent, Canterbury, UK.
authors.forename: Kym N
authors.initials: KN
authors.lastname: Baker
authors.forename: Mark H
authors.initials: MH
authors.lastname: Rendall
authors.forename: Ashvin
authors.initials: A
authors.lastname: Patel
authors.forename: Paul
authors.initials: P
authors.lastname: Boyd
authors.forename: Mike
authors.initials: M
authors.lastname: Hoare
authors.forename: Robert B
authors.initials: RB
authors.lastname: Freedman
authors.forename: David C
authors.initials: DC
authors.lastname: James
mesh_terms: D015374:Biosensing Techniques / Q000191:economics / Q000379:methods / Q000639:trends; D004563:Electrochemistry / Q000191:economics / Q000295:instrumentation / Q000639:trends; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods / Q000639:trends; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D009391:Nephelometry and Turbidimetry / Q000191:economics / Q000379:methods / Q000639:trends; D055095:Optics and Photonics / Q000295:instrumentation; D011994:Recombinant Proteins / Q000032:analysis*
chemical_list: D011994:Recombinant Proteins