title: Optimization of the enzymatic synthesis of O-glycan core 2 structure by use of a genetic algorithm.
abstract: The enzymatic synthesis of Gal-beta 1,3[GlcNAc-beta 1,6]-GalNAc-alpha 1-OBn (core 2-Bn) using a multi-enzyme system consisting of a beta-galactosidase (EC 3.2.1.23) from bovine testes and a recombinant core 2 beta 1,6-GlcNAc transferase (C2GnT, EC 2.4.1.102) was empirically optimized by the use of a genetic algorithm. After variation of seven relevant parameters and performance of 56 experiments, two local maxima regarding the selection criteria could be found after four generations of optimization. The selectivity of core 2-Bn formation showed values up to 90%.
authors.affiliation: Forschungszentrum Jülich GmbH, Institut für Biotechnologie 2, D-52425 Jülich, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Hoh
authors.forename: Gregor
authors.initials: G
authors.lastname: Dudziak
authors.forename: Andreas
authors.initials: A
authors.lastname: Liese
mesh_terms: D000465:Algorithms; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002417:Cattle; D008297:Male; D008957:Models, Genetic; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry* / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013737:Testis / Q000201:enzymology*; D014312:Trisaccharides / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D009844:Oligosaccharides; D011134:Polysaccharides; D014312:Trisaccharides; C073212:galactosyl beta(1-3)-N-acetylglucosaminyl-beta(1-6)-N-acetylgalactosamine; D017351:N-Acetylglucosaminyltransferases; C053024:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase; D001616:beta-Galactosidase