Studies on serological cross-reaction of Neospora caninum with Toxoplasma gondii and Hammondia heydorni.
The enzyme-linked immunosorbent assay (ELISA) was used to examine cross-reactivity of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. Anti-T. gondii mouse and cat sera cross-reacted with N. caninum soluble antigen (NLA), but not with the recombinant surface antigen (NcSRS2). Anti-H. heydorni dog sera showed no cross-reactivity with either the NLA antigen or the NcSRS2. Lack of cross-reactivity between anti-H. heydorni sera and N. caninum antigens, and the cross-reactivity of anti-T. gondii sera with the NLA suggest that N. caninum has common antigens to T. gondii except for NcSRS2 based on serology. In light of several studies suggesting a closer relationship between N. caninum and H. heydorni than with T gondii, examination of serological cross-reactivity with N. caninum may be necessary to further classify the parasites in addition to molecular and morphological studies and clarification of the life cycle.
Nishikawa
Yoshifumi
Y
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inadacho, Hokkaido, Japan.
Claveria
Florencia G
FG
Fujisaki
Kozo
K
Nagasawa
Hideyuki
H
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions; D021741:Eimeriida / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018688:Neospora / Q000145:classification / Q000276:immunology*; D010802:Phylogeny; D014122:Toxoplasma / Q000145:classification / Q000276:immunology*
D000953:Antigens, Protozoan