Glucose deprivation and hypoxia increase the expression of the GLUT1 glucose transporter via a specific mRNA cis-acting regulatory element.
The expression of the glucose transporter type-1 (GLUT1) gene is up-regulated in hypoxia and glucose deprivation. A 10 nucleotide (nt) cis-acting regulatory element (CAE), which is located within nt 2181-2190 of the GLUT1 3'-untranslated region (CAE2181-2190), increases the expression of a GLUT1-luciferase reporter gene and decreases its mRNA decay. The present study investigated the role of the GLUT1 CAE2181-2190 in glucose deprivation and hypoxia using stable transfectants. Glucose and O2 deprivation produced a marked increase in the expression of the GLUT1 reporter gene carrying the CAE2181-2190, and this effect was additive. Glucose deprivation and/or hypoxia induced no significant changes in the expression of the reporter gene wherein the GLUT1 CAE2181-2190 was site-directed deleted. Data presented here suggest that the GLUT1 CAE2181-2190 participates in the increase of GLUT1 gene expression in glucose deprivation and hypoxia.
Boado
Ruben J
RJ
Department of Medicine and Brain Research Institute, UCLA School of Medicine, Los Angeles, California 90024, USA. rboado@med1.medsch.ucla.edu
Pardridge
William M
WM
D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology; D015687:Cell Hypoxia / Q000502:physiology; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000494:pharmacology*; D051272:Glucose Transporter Type 1; D007003:Hypoglycemia / Q000503:physiopathology; D002534:Hypoxia, Brain / Q000503:physiopathology; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495679:Slc2a1 protein, rat; D005947:Glucose; D010100:Oxygen