Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus.
The complete genome of spring viremia of carp virus (SVCV) was cloned and the sequence of 11019 nucleotides was determined. It contains five open reading frames (ORF's) encoding for the nucleoprotein N; phosphoprotein P; matrix protein M; glycoprotein G; and the viral RNA dependent RNA polymerase L. Genes are organised in the order typical for rhabdoviruses: 3'-N-P-M-G-L-5'. The short leader and trailer regions of SVCV exhibit inverse complementarity and are similar to the respective 3' and 5' ends of the genome of vesicular stomatitis virus. To verify the predicted open reading frames proteins were expressed in bacteria and analysed with a polyclonal anti-SVCV serum. Furthermore, monospecific antisera against the distinct viral proteins were generated. Comparison of genome and protein confirm the assignment of SVCV to the genus Vesiculovirus.
Hoffmann
Bernd
B
Institute of Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.
Schütze
Heike
H
Mettenleiter
Thomas C
TC
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002347:Carps / Q000821:virology*; D002460:Cell Line; D004279:DNA, Viral; D004926:Escherichia coli; D005393:Fish Diseases / Q000821:virology; D015870:Gene Expression; D005814:Genes, Viral; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D012209:Rhabdoviridae / Q000235:genetics*; D018353:Rhabdoviridae Infections / Q000662:veterinary / Q000821:virology; D014763:Viral Matrix Proteins / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D004279:DNA, Viral; D006023:Glycoproteins; D014763:Viral Matrix Proteins; D012324:RNA-Dependent RNA Polymerase