title: The effects of platelet apheresis in total hip replacement surgery on platelet activation.
abstract: BACKGROUND
  Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.
  
  
  METHODS
  Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group. Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry.
  
  
  CONCLUSIONS
  A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1-23% in peroperative activation. PRP harvest did not significantly alter platelet activity. The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value +/- SD 86% +/- 7.5%).
authors.affiliation: Department of Anesthesiology, Orebro Medical Center Hospital, Orebro, Sweden. gustav.ekback@orebroll.se
authors.forename: G
authors.initials: G
authors.lastname: Ekbäck
authors.forename: B
authors.initials: B
authors.lastname: Edlund
authors.forename: A
authors.initials: A
authors.lastname: Smolowicz
authors.forename: K
authors.initials: K
authors.lastname: Axelsson
authors.forename: J
authors.initials: J
authors.lastname: Kjellberg
authors.forename: O
authors.initials: O
authors.lastname: Carlsson
authors.forename: U
authors.initials: U
authors.lastname: Schött
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000368:Aged; D019644:Arthroplasty, Replacement, Hip; D001792:Blood Platelets / Q000737:chemistry; D001804:Blood Transfusion, Autologous; D006801:Humans; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D015539:Platelet Activation / Q000187:drug effects; D010983:Plateletpheresis; D011446:Prospective Studies
chemical_list: D019007:P-Selectin; D000244:Adenosine Diphosphate