Structural varieties of small proteoglycans in human spinal ligament.
Three types of small proteoglycan were purified from human spinal ligaments by ultracentrifugation, ion-exchange chromatography, gel-chromatography, and hydrophobic chromatography. Two of them were identified as decorin and biglycan, and the other was thought to be a decorin-subtype. Molecular sizes of decorin and decorin-subtype were both 85 kDa, and that of biglycan was 200 kDa. N-Terminal amino acid sequence of decorin-subtype corresponded with that of decorin, although it was different from decorin in terms of composition of amino acids and glycosaminoglycan chains, and reactivity with anti-human decorin antibody. The ratios of chondroitin sulfate to dermatan sulfate contained in the three proteoglycans were different, and the location of that in glycosaminoglycan chains was also thought to be different. It was demonstrated that three types of proteoglycan which are structurally different are present in extracellular matrix.
Yukawa
M
M
Department of Biochemistry, Hirosaki University, School of Medicine, Japan.
Takagaki
K
K
Itabashi
T
T
Ueyama
K
K
Harata
S
S
Endo
M
M
D000595:Amino Acid Sequence / Q000502:physiology; D058578:Biglycan; D002114:Calcinosis / Q000378:metabolism* / Q000503:physiopathology; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006801:Humans; D017843:Ligamentum Flavum / Q000737:chemistry*; D015394:Molecular Structure; D008970:Molecular Weight; D020033:Protein Isoforms / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry*; D013131:Spine / Q000033:anatomy & histology / Q000737:chemistry*
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D020033:Protein Isoforms; D011506:Proteins; D011509:Proteoglycans