Inhibition of cancer cell growth by BRCA2.
The breast cancer susceptibility gene BRCA2 has been suggested to function as a "caretaker" of the genome. Cells without wild-type BRCA2 are deficient in repairing DNA damage. However, whether BRCA2 can also suppress oncogenesis by regulating cell proliferation remains to be determined. To address this question, the expression of wild-type BRCA2 protein was reconstituted, in an either constitutive or regulated manner, in the pancreatic cancer cell line Capan-1, which expresses only a mutant BRCA2. Expression of wild-type BRCA2 inhibited cell proliferation in culture and suppressed tumor growth in animals. Our results showed that, in addition to the DNA repair function, BRCA2 also suppresses tumor development by inhibiting cancer cell growth.
Wang
Shao-Chun
SC
Department of Molecular and Cellular Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
Shao
Ruping
R
Pao
Annie Y
AY
Zhang
Su
S
Hung
Mien-Chie
MC
Su
Li-Kuo
LK
D000818:Animals; D002455:Cell Division; D005260:Female; D024522:Genes, BRCA2 / Q000502:physiology*; D051379:Mice; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D014162:Transfection; D014407:Tumor Cells, Cultured