Decreased survival of UV-irradiated Staphylococcus aureus in the presence of 8-methoxypsoralen in the post-irradiation plating medium.
For Staphylococcus aureus, the presence of 8-methoxypsoralen (8-MOP) in the post-irradiation plating medium increased the lethal effect of far-UV light (FUV; approximately 254 nm) and of 8-MOP plus near-UV light (8-MOP+NUV; approximately 365 nm), an effect similar to that caused by acriflavine which inhibits DNA repair. In the repair-proficient strain, the presence of 8-MOP in the plating medium was almost as effective in inhibiting the repair of damage caused by FUV as that caused by 8-MOP photoadditions. Survival data obtained with Rec(-)-like and Uvr(-)-like strains suggest that 8-MOP in the plating medium, although possibly inhibiting recombination repair, was much more effective in inhibiting excision repair of FUV damage. Regarding 8-MOP+NUV treatment, 8-MOP in the plating medium had a lesser effect in the repair-deficient strains, differing from that observed after FUV treatment, which is consistent with the notion that different types of damage are caused by the two treatments.
Barros
S M D
SM
Departamento de Biologia Molecular/CCEN, Universidade Federal da Paraíba, 58059-900 João Pessoa (PB), Brazil.
Siqueira-Junior
J P
JP
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D008730:Methoxsalen / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
D000900:Anti-Bacterial Agents; D003470:Culture Media; D008730:Methoxsalen