title: ATP synthesis in the disk membranes of rod outer segments of bovine retina.
abstract: ATP is synthesized on the disk membrane isolated from rod outer segments of the bovine retina. Together with a slow component which accounted for a constant rate of about 22 nmol ATP/min/mg of protein and which was due to the adenylate kinase activity, a fast component with a maximal activity of about 58 nmol ATP/min/mg of protein was measured at physiological calcium concentrations. This fast activity disappeared in the presence of the Ca(2+) ionophore A23187, was inhibited by vanadate or thapsigargin but not by oligomycin, suggesting that this ATP synthesis is due to the reversal functioning of the Ca(2+)-ATPase previously found on the disk membranes.
authors.affiliation: Istituto di Biofisica, Facoltá di Medicina, Universitá di Genova, Corso Europa, I-16132 Genoa, Italy. pepe@ibf.unige.it
authors.forename: I M
authors.initials: IM
authors.lastname: Pepe
authors.forename: L
authors.initials: L
authors.lastname: Notari
authors.forename: C
authors.initials: C
authors.lastname: Cugnoli
authors.forename: I
authors.initials: I
authors.lastname: Panfoli
authors.forename: A
authors.initials: A
authors.lastname: Morelli
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D012374:Rod Cell Outer Segment / Q000187:drug effects / Q000201:enzymology*; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D007476:Ionophores; D000001:Calcimycin; D000244:Adenosine Diphosphate; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium