Cloning and expression of Ventrhoid, a novel vertebrate homologue of the Drosophila EGF pathway gene rhomboid.
In Drosophila melanogaster, the seven-pass transmembrane protein Rhomboid (Rho) is a crucial positive modulator of EGF signaling playing a substantial role in patterning of the ventral neuroectoderm and fate specification of neuroblasts. Here, we describe the cloning and expression pattern of Ventrhoid (Vrho), the novel evolutionarily conserved vertebrate cDNA related to fruit fly rho. Most importantly, like rho in Drosophila, Vrho is also expressed in a spatially restricted manner. Vrho expression is most prominent along the developing ventral neural tube, and is also detectable in the ventral forebrain, prospective diencephalon, otic vesicles, mandibular arches, cranial sensory placodes, last formed pair of somites and hindgut in midgestational mouse embryos.
Jászai
József
J
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
Brand
Michael
M
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000196:embryology; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D007438:Introns; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation
D018076:DNA, Complementary; D029721:Drosophila Proteins; D008565:Membrane Proteins; C081481:Rho protein, Drosophila; C472498:Stet protein, Drosophila; D004247:DNA; C466104:RHBDL3 protein, human