title: Loss of phosphotyrosine phosphatase activity and changes in the tyrosine phosphorylation state of proteins after storage of sheep platelets in plasma or Seto solution at 4 degrees C.
abstract: OBJECTIVE
  During platelet storage an array of deleterious changes occur, through mechanisms not fully understood, which impair platelet haemostasis. Transfused platelets should maintain the integrated networks of signalling pathways that regulate platelet activation and functionality. We hypothesized that protein phosphorylation and dephosphorylation, which play a fundamental role in these pathways, might be affected by platelet storage. We therefore investigated whether the activity of phosphotyrosine phosphatase (PTP), which belongs to an oxidant-susceptible group of enzymes involved in the platelet signal-transduction pathways that ensure platelet functionality, is affected by platelet storage.
  
  
  METHODS
  Using sheep platelet species as a model system, we conducted serial studies on the membranes of platelets and microparticles shed during platelet storage, in their own plasma or in a synthetic medium called Seto, for up to 5 days at 4 degrees C.
  
  
  RESULTS
  A progressive decrease in both total and specific membrane-associated PTP activities from whole platelets (but not from microparticles) located within each platelet storage bag was observed from day 1 onwards in both types of storage media. These decreases could be partly avoided by the addition of vitamin E. Additionally, the observed decrease in PTP activity was accompanied with increases in the tyrosine phosphorylation of proteins from whole platelets or crude platelet membranes, the tyrosine phosphorylation state of proteins from microparticles remaining basically unchanged.
  
  
  CONCLUSIONS
  Our findings suggest that alterations of at least the tyrosine phosphorylation balance might be one of the reasons for the decrease in the haemostatic function of stored platelets.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Salamanca, 106 Plaza Doctores de la Reina, E-37007 Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Hernández-Hernández
authors.forename: C
authors.initials: C
authors.lastname: Sánchez-Bernal
authors.forename: M C
authors.initials: MC
authors.lastname: Rodríguez
authors.forename: F P
authors.initials: FP
authors.lastname: Gómez
authors.forename: M
authors.initials: M
authors.lastname: Llanillo
authors.forename: J
authors.initials: J
authors.lastname: Sánchez-Yagüe
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods*; D015925:Cryopreservation; D005950:Glucose Solution, Hypertonic / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D023421:Models, Animal; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012756:Sheep; D014443:Tyrosine / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D005950:Glucose Solution, Hypertonic; D008565:Membrane Proteins; D010750:Phosphoproteins; C076429:Seto solution; D014810:Vitamin E; D014443:Tyrosine; D017027:Protein Tyrosine Phosphatases