The unique centromeric chromatin structure of Schizosaccharomyces pombe is maintained during meiosis.
In meiosis I sister centromeres are unified in their polarity on the spindle, and this unique behavior is known to require the function of meiosis-specific factors that set some intrinsic property of the centromeres. The fission yeast, Schizosaccharomyces pombe, possesses complex centromeres consisting of repetitive DNA elements, making it an excellent model in which to study the behavior of complex centromeres. In mitosis, during which sister centromeres mediate chromosome segregation by establishing bipolar chromosome attachments to the spindle, the central core of the S. pombe centromere chromatin has a unique irregular nucleosome pattern. Deletion of repeats flanking this core structure have no effect on mitotic chromosome segregation, but have profound effects during meiosis. While this demonstrates that the outer repeats are critical for normal meiotic sister centromere behavior, exactly how they function and how monopolarity is established remains unclear. In this study we provide the first analysis of the chromatin structure of a complex centromere during meiosis. We show that the nature and extent of the unique central core chromatin structure is maintained with no measurable expansion. This demonstrates that monopolarity of sister centromeres, and subsequent reversion to bipolarity, does not involve a global change to the centromeric chromatin structure.
Smirnova
Julia B
JB
Molecular and Cell Biology Group, School of Biological Sciences, Memorial Building, University of Wales-Bangor, Deiniol Road, Bangor, Gwynedd LL57 2UW, United Kingdom.
McFarlane
Ramsay J
RJ
D002503:Centromere / Q000378:metabolism / Q000648:ultrastructure*; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D002875:Chromosomes / Q000648:ultrastructure; D004171:Diploidy; D008540:Meiosis; D008836:Micrococcal Nuclease / Q000494:pharmacology; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D012568:Schizosaccharomyces / Q000502:physiology*
D002843:Chromatin; D008836:Micrococcal Nuclease