title: Uterine responses to exogenous oxytocin before and after pre-partum luteolysis in the cow.
abstract: The aim of this study was to test the functional status of uterine oxytocin receptors in cows in vivo around parturition. The animals received consecutive, intra-arterial injections of 800, 1,600 and 3,200 mU of oxytocin at three different stages: during late gestation (days 260-274), at 12 h and at 24 h after intramuscular injection of a prostaglandin F2alpha analogue at day 275 to induce parturition. Cows (n = 6) had been provided with myometrial electrodes and a catheter had been installed in the aorta and in a branch of the uterine vein (UV). Regular blood samples were obtained from the UV from 5 min before until 45 min after each oxytocin injection to measure plasma levels of prostaglandin F2alpha (PGF2alpha) and oxytocin. Uterine electromyographic (EMG) activity was registered continuously during each experiment. The increase of oxytocin levels in UV plasma after intra-arterial injections was dose dependent (p < 0.02). Pre- and post treatment oxytocin levels at 24 h after induction of parturition were significantly increased (p = 0.0313). Both during late pregnancy and at 12 h after induction of parturition, oxytocin caused a significant increase in EMG activity (p = 0.022). After the 3,200 mU dose the increase was significantly higher than with the other 2 doses (p = 0.004). After each dose, EMG activity returned to baseline levels within some 15 min. At 24 h after induction of parturition, the pre-treatment level of EMG activity had increased. Doses of 800 mU and 1,600 mU of oxytocin produced a significant (p = 0.022) increment of EMG activity, which was of the same magnitude as during the preceding stages; after 3,200 mU of oxytocin the response was significantly higher than before (p = 0.008). No significant increases of PGF2alpha levels in UV plasma could be measured after oxytocin injections at any of the three stages. It is concluded that the myometrium of the pregnant cow responds in vivo to physiological doses of oxytocin. At 24 h after induction of parturition, when luteolysis has occurred and a parturient pattern of parturient myometrial activity has already started to develop, the response is enhanced. Physiological doses of oxytocin did not evoke a spurt release of PGF2alpha in uterine venous blood during the peripartal period.
authors.affiliation: Department of Herd Health and Reproduction, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. m.a.m.taverne@vet.uu.nl
authors.forename: M M
authors.initials: MM
authors.lastname: Taverne
authors.forename: N C
authors.initials: NC
authors.lastname: de Schwartz
authors.forename: M
authors.initials: M
authors.lastname: Kankofer
authors.forename: M M
authors.initials: MM
authors.lastname: Bevers
authors.forename: H A
authors.initials: HA
authors.lastname: van Oord
authors.forename: D
authors.initials: D
authors.lastname: Schams
authors.forename: S
authors.initials: S
authors.lastname: Gutjahr
authors.forename: G C
authors.initials: GC
authors.lastname: van der Weijden
mesh_terms: D000818:Animals; D002417:Cattle; D015237:Dinoprost / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004576:Electromyography / Q000662:veterinary; D005260:Female; D007751:Labor, Induced / Q000662:veterinary; D003341:Luteolysis; D009215:Myometrium / Q000187:drug effects; D010121:Oxytocin / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D011247:Pregnancy; D014590:Uterine Contraction / Q000187:drug effects* / Q000502:physiology
chemical_list: D010121:Oxytocin; D015237:Dinoprost