Gamma-interferon and soluble interleukin 2 receptor in tuberculous pleural effusion.
To analysis the difference between systemic and local pleural T cell response in pulmonary tuberculosis, we analyzed interferon (IFN)-gamma and soluble interleukin-2 receptor (sIL-2R) in peripheral blood mononuclear cells (PBMC) culture supernatants and in pleural effusion (PE). We also investigated the association of pleural INF-gamma and sIL-2R levels with development of residual pleural thickening (RPT). The subjects in this study included patients with active pulmonary tuberculosis with or without PE (n = 46), those with nontuberculous PE (n = 32), and healthy tuberculin reactors (n = 20). Measurement of IFN-gamma and sIL-2R were made by ELISA. In pulmonary tuberculosis, IFN-gamma and sIL-2R concentrations in PBMC culture supernatants were lower than those of healthy tuberculin reactors (IFN-gamma; 258.4 +/-111.5 pg/mL versus 2792.5 +/-633.2 pg/mL, sIL-2R; 1465.0 +/-144.4 pg/mL versus 4777.1 +/-178.5 pg/mL, p < 0.05), whereas IFN-gamma and sIL-2R concentrations in PE were higher than those from nontuberculous pleural effusion (IFN-gamma; 1154.4 +/-252.4 pg/mL versus 292.0 +/-68.9 pg/mL, sIL-2R; 9805.2 +/-978.9 pg/mL versus 3426.7 +/-695.6 g/mL, p < 0.05). IFN-gamma and sIL-2R in PBMC culture supernatants were significantly lower in tuberculat patients with PE than those without PE, and the patients with a high value of IFN-gamma or sIL-2R in PE showed a low value of IFN-gamma or sIL-2R in PBMC culture supernatant, respectively. Patients with RPT had significantly higher IFN-gamma and sIL-2R values in their PE compared with those without RPT. These findings suggest that diminished systemic Th1 response in tuberculosis results from the accumulation of activated Th1 cell to the disease site, and that levels of IFN-gamma and sIL-2R in PE are useful posttreatment markers of RPT.
Kim
Y K
YK
Department of Internal Medicine, Kangnam St. Mary's Hospital, Catholic University School of Medicine, 505 Banpo-Dong, Seocho-Ku, Seoul 137-040, Korea.
Lee
S Y
SY
Kwon
S S
SS
Kim
K H
KH
Moon
H S
HS
Song
J S
JS
Park
S H
SH
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D003937:Diagnosis, Differential; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008875:Middle Aged; D010996:Pleural Effusion / Q000097:blood* / Q000175:diagnosis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000097:blood*; D012995:Solubility; D014396:Tuberculosis, Pleural / Q000097:blood* / Q000175:diagnosis; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000473:pathology
D015415:Biomarkers; D015375:Receptors, Interleukin-2; D007371:Interferon-gamma