title: Simultaneous expression of allogenic class II MHC and B7.1 (CD80) molecules in A20 B-lymphoma cell line enhances tumor immunogenicity.
abstract: We expressed the allogenic class II MHC antigen and B7.1 (CD80) co-stimulatory molecule in A20 beta-lymphoma cells in order to test their efficacy as immuno-stimulating adjuvant agents in inducing tumor-specific immunity. The transduction of the allogenic I-Ab alpha and beta chain genes into A20 cell resulted in a surface expression of the allogenic class II MHC molecules. The expression of the allogenic class II MHC antigen (I-Ab) in A20 cells enhanced the proliferation of T cells in a mixed lymphocyte tumor culture and in vitro cytotoxic T lymphocyte (CTL) generation against parental cells. The B7.1 gene, which is known to be a potent co-stimulatory molecule, was also transduced and expressed in A20 cells, either alone or in combination with I-Ab. The B7.1 transduction alone leads to a similar in vitro immune enhancing effect as I-Ab. When both the I-Ab and B7.1 genes were transduced, the in vitro immunostimulating capacity was further enhanced. Finally, we also tested the A20 cells that were transduced with I-Ab and/or B7.1 for their efficacy as preventive tumor vaccines in vivo. The results indicate that the A20 cells that express both the I-Ab and B7.1 have more potent vaccinating potential, compared to the cells that express only one of the molecules.
authors.affiliation: Department of Microbiology, College of Medicine, Hanyang University, Seoul, Korea.
authors.forename: Young-Ju
authors.initials: YJ
authors.lastname: Jang
authors.forename: Seon Young
authors.initials: SY
authors.lastname: Nam
authors.forename: Moon-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Rho Hyun
authors.initials: RH
authors.lastname: Seong
authors.forename: Yong-Su
authors.initials: YS
authors.lastname: Park
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Chung
authors.forename: Hee-Yong
authors.initials: HY
authors.lastname: Chung
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism*; D019496:Cancer Vaccines / Q000235:genetics / Q000276:immunology; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology / Q000628:therapy; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D018122:B7-1 Antigen; D019496:Cancer Vaccines; D000949:Histocompatibility Antigens Class II