IL-6 a key cytokine in in vitro and in vivo response of Sertoli cells to external gamma irradiation.
Interleukin 1(IL-1) and IL-6 are cytokines involved in the response to radiation and are known for their radioprotective properties with respect to total-body irradiation. We previously showed that after gamma irradiation of Sertoli cells (SC), we observed an increase in the activity of IL-6 but not of IL-1. The aim of this study was to see whether this response is a function of the differentiation of SC, to analyse the mechanisms responsible for this induction, and to test whether this cytokine has a radioprotective role on germ cells. Unlike IL-1, a dose-dependent increase of IL-6 activity in SC following gamma irradiation at high doses was observed at all ages studied. On the other hand, radio-induction observed at low doses (<1Gy) was dose-independent. IL-6 up-regulation resulted from transcriptional activation as shown by the use of specific inhibitors. The injection of IL-1 and IL-6 in mice prior to whole-body irradiation resulted in an increased survival rate. Moreover, cytokines protected DNA from remaining cells following irradiation as shown by comet assay on germ cells. In conclusion, IL-6 seems to constitute a good marker of exposure to gamma irradiation, both at low and high doses. In addition, we showed that IL-1 and IL-6 have a radioprotective effect at testicular level.
Legué
F
F
UPRES EA 2231, Université de Rennes I, Centre Eugène Marquis, CS 44229, 35042 Rennes Cedex, France.
Guitton
N
N
Brouazin-Jousseaume
V
V
Colleu-Durel
S
S
Nourgalieva
K
K
Chenal
C
C
D000818:Animals; D002470:Cell Survival; D020552:Comet Assay; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012708:Sertoli Cells / Q000378:metabolism* / Q000528:radiation effects*; D013737:Testis / Q000528:radiation effects; D013997:Time Factors
D007375:Interleukin-1; D015850:Interleukin-6; D011500:Protein Synthesis Inhibitors; D011838:Radiation-Sensitizing Agents; D011994:Recombinant Proteins; D003609:Dactinomycin; D003513:Cycloheximide