title: Facilitation of antibody forming responses to viral vaccine antigens in young cats by recombinant baculovirus-expressed feline IFN-gamma.
abstract: We assessed the effect of recombinant feline IFN-gamma as vaccine adjuvant for in vivo antibody responses of young 3-month-old kittens to inactivated antigens of rabies and calicivirus, both natural pathogens for cats. When compared to responses following immunization with antigen alone co-administration of baculovirus-expressed cat IFN-gamma significantly enhanced serum antibody titers to both viral antigens; to levels comparable with responses evoked by commonly known saponin and alum adjuvants. Adjuvanticity by feline IFN-gamma was dose-dependent and all doses tested were well tolerated. We conclude that, when further optimized for in vivo delivery, feline IFN-gamma may represent a safe and efficient natural vaccine adjuvant for certain antigens in cats.
authors.affiliation: Department of Vaccine Technology and Immunology, Intervet Int. BV, P.O. Box 31, 5830 AA Boxmeer, The Netherlands. virgil.schijns@intervet.com
authors.forename: Virgil E J C
authors.initials: VE
authors.lastname: Schijns
authors.forename: Nicolette C
authors.initials: NC
authors.lastname: Scholtes
authors.forename: Hanneke I van
authors.initials: HI
authors.lastname: Zuilekom
authors.forename: Lisette E H H
authors.initials: LE
authors.lastname: Sanders
authors.forename: Lesley
authors.initials: L
authors.lastname: Nicolson
authors.forename: David J
authors.initials: DJ
authors.lastname: Argyle
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000008:administration & dosage; D016367:Baculoviridae / Q000235:genetics; D017927:Calicivirus, Feline / Q000276:immunology; D002415:Cats; D007371:Interferon-gamma / Q000008:administration & dosage* / Q000235:genetics; D011819:Rabies Vaccines / Q000008:administration & dosage; D011994:Recombinant Proteins; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011819:Rabies Vaccines; D011994:Recombinant Proteins; D014765:Viral Vaccines; D007371:Interferon-gamma