title: Preparation of (1-->4)-beta-D-xylooligosaccharides from an acid hydrolysate of cotton-seed xylan: suitability of cotton-seed xylan as a starting material for the preparation of (1-->4)-beta-D-xylooligosaccharides.
abstract: Cotton-seed residual cake, which is a byproduct of the process of oil extraction from the seed, was delignified with sodium hypochlorite (1% available chlorine). Xylan was then prepared from the delignified wet material by alkali extraction with 15% sodium hydroxide. The cotton-seed xylan contained 64.7% xylose and 9.4% uronic acid. The xylan was hydrolyzed with 0.125 M sulfuric acid at 90 degrees C for 15 min. The resultant hydrolysis products were separated by gel-permeation chromatography on BioGel P-4 and Toyopearl HW-40F columns connected in series, with water as an eluate. Xylose and xylooligosaccharides with a degree of polymerization ranging from DP 2 to 15 were separated under such conditions, and each xylooligosaccharide-containing peak fraction afforded a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that cotton-seed xylan is suitable for the preparation of xylose and xylooligosaccharides.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba-shi, 1-1-1 Tennoodai, Ibaraki 305-8572, Japan.
authors.forename: Hyeon Jin
authors.initials: HJ
authors.lastname: Sun
authors.forename: Shigeki
authors.initials: S
authors.lastname: Yoshida
authors.forename: Nyun Ho
authors.initials: NH
authors.lastname: Park
authors.forename: Isao
authors.initials: I
authors.lastname: Kusakabe
mesh_terms: D003368:Gossypium / Q000737:chemistry*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds / Q000737:chemistry; D014990:Xylans / Q000737:chemistry*; D014994:Xylose / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D009844:Oligosaccharides; D014990:Xylans; D014994:Xylose