Development of a CE method to analyze organic acids in dairy products: application to study the metabolism of heat-shocked spores.
Organic acids are relevant in dairy products for nutritional reasons and because they contribute to the flavor and aroma. They are the major products of carbohydrate catabolism of lactic acid bacteria and nonstarter bacteria associated with milk. In several research and quality programs, it is very important to develop a rapid and sensitive method for their quantitative determination in dairy products to monitor bacterial growth and activity. A capillary electrophoresis method for the simultaneous determination of oxalic, citric, formic, succinic, orotic, uric, pyruvic, acetic, propionic, lactic, and butyric acid in less than 18 min has been developed. Various parameters affecting analysis, including capillary length, type, composition, and pH of the electrolyte have been optimized. Some alternatives are given to improve the separation of particular organic acids of special interest. Its application to analyze the quality of some dairy products has been investigated. In addition, the suitability of the technique to determine profiles of organic acids generated during the metabolism of heat-shocked spores has been demonstrated.
Izco
Jesús M
JM
Dairy Products Technology Center, California Polytechnic State University, 1 Grand Avenue, San Luis Obispo, California 93407, USA. jesus.izco@unavarra.es
Tormo
Mónica
M
Jiménez-Flores
Rafael
R
D019342:Acetic Acid / Q000032:analysis; D000818:Animals; D020148:Butyric Acid / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D019343:Citric Acid / Q000032:analysis; D003611:Dairy Products / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005561:Formates / Q000032:analysis; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000032:analysis; D009963:Orotic Acid / Q000032:analysis; D019815:Oxalic Acid / Q000032:analysis; D011422:Propionates / Q000032:analysis; D019289:Pyruvic Acid / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism*; D019802:Succinic Acid / Q000032:analysis; D014527:Uric Acid / Q000032:analysis
D002264:Carboxylic Acids; D005561:Formates; D011422:Propionates; C030544:formic acid; D020148:Butyric Acid; D014527:Uric Acid; D019343:Citric Acid; D019344:Lactic Acid; D009963:Orotic Acid; D019289:Pyruvic Acid; D019815:Oxalic Acid; D019802:Succinic Acid; C029658:propionic acid; D019342:Acetic Acid