Prevalence of a novel capsule-associated lipoprotein among pasteurellaceae pathogenic in animals.
Capsular serotype A strains of Pasteurella multocida of avian origin express a 40-kDa lipoprotein (Plp-40) thought to attach the extracellular polysaccharide to the cell surface. The objective of the present study was to assess the prevalence of Plp-40 in P. multocida strains of disparate serotypes and host origins, as well as other pathogenic members of the family Pasteurellaceae. Exponential-phase reference and clinical isolates were radiolabeled with [3H]-palmitate, lysed to obtain whole-cell protein fractions, and analyzed using SDS-PAGE and fluorography to assess lipoprotein content. The ability to produce Plp-40 was found to be conserved among certain P. multocida reference and clinical strains of different host origins including avian, human, porcine, bovine, feline, canine, ovine, and cervine, but not rabbit. Production of a 40-kDa lipoprotein was exhibited by all clinical isolates of Pasteurella aerogenes, Pasteurella pneumotropica, Actinobacillus suis, Actinobacillus suis-like organism, and Actinobacillus pleuropneumoniae examined, but not Pasteurella (Mannheimia) haemolytica, Actinobacillus lignieresii, or Haemophilus spp. These data suggest that, while not all Pasteurellaceae are able to produce a 40-kDa lipoprotein under the present experimental conditions, expression is somewhat conserved among diverse isolates of disparate host origins.
Champlin
Franklin R
FR
Department of Biological Sciences, Mississippi State University, P.O. Box GY, Mississippi State, MS 39762, USA. franko@biology.msstate.edu
Shryock
Thomas R
TR
Patterson
Charles E
CE
Austin
Frank W
FW
Ryals
Phillip E
PE
D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry*; D001717:Birds; D002415:Cats; D002417:Cattle; D002645:Chickens; D003670:Deer; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005289:Ferrets; D006801:Humans; D008074:Lipoproteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D010326:Pasteurella Infections / Q000662:veterinary*; D016979:Pasteurella multocida / Q000737:chemistry; D015661:Pasteurellaceae / Q000737:chemistry* / Q000472:pathogenicity; D011817:Rabbits; D051381:Rats; D012703:Serotyping; D012756:Sheep; D013552:Swine; D014422:Turkeys
D008074:Lipoproteins