Expression of the helix-loop-helix gene id3 in the zebrafish embryo.
Proteins of the Extramacrochaetae and Id subfamily of Helix-Loop-Helix (HLH) proteins are negative regulators of bHLH transcription factors. We cloned a cDNA from zebrafish which encodes a member of the id3 subfamily. High levels of transcripts accumulated in the germ ring and in the embryonic shield. Towards the end of gastrulation, Id3 was highly expressed in the anterior prechordal plate and hypoblast. At later stages, id3 expression was turned on and off in a large variety of tissues within short periods of time. These include the lateral mesoderm, the cornea, the lens, the brain, the neural crest, the retina and the fins.
Dickmeis
Thomas
T
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, F-67404 Illkirch Cedex, C.U. de Strasbourg, France.
Rastegar
Sepand
S
Lam
Chen Sok
CS
Aanstad
Pia
P
Clark
Matthew
M
Fischer
Nadine
N
Rosa
Frédéric
F
Korzh
Vladimir
V
Strähle
Uwe
U
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018257:Helix-Loop-Helix Motifs; D007150:Immunohistochemistry; D051796:Inhibitor of Differentiation Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000196:embryology; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D015027:Zebrafish
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051796:Inhibitor of Differentiation Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C075640:ID3 protein, human