title: Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences.
abstract: The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes in bacilli by binding to the leader region of the nascent trp operon mRNA. When activated by binding tryptophan, the 11-subunit circular TRAP molecule binds to a target sequence consisting of 11 (G/U)AG repeats, separated by two or three variable 'spacer' nucleotides. Reported here are two crystal structures of TRAP bound to RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the spacer regions of the RNA molecules to be highly flexible, making no direct hydrogen-bonding contacts with the protein. Comparison of these structures with the previous structure of TRAP bound to (GAGAU)(10)GAG RNA, in which the spacer nucleotides stack with each other close to the protein surface, shows that the RNA can adopt different conformations depending on the sequence of the spacer regions. This gives insight into the structural basis of the specificity of TRAP and into the mechanism of binding.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, York Y010 5DD, England.
authors.forename: Nicholas H
authors.initials: NH
authors.lastname: Hopcroft
authors.forename: Alice L
authors.initials: AL
authors.lastname: Wendt
authors.forename: Paul
authors.initials: P
authors.lastname: Gollnick
authors.forename: Alfred A
authors.initials: AA
authors.lastname: Antson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001483:Base Sequence; D018360:Crystallography, X-Ray; D001411:Geobacillus stearothermophilus / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000737:chemistry*; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors