Spatial arrangement of proteins within the small subunit of rat liver ribosomes studied by cross-linking.
Cross-linking of proteins within the small subunit of rat liver ribosomes by the bifunctional reagent dimethyl 4,7-dioxo-5,6-dihydroxy-3,8-diazadecanbisimidate produced numerous covalently linked protein dimers which could be separated by a combination of ion-exchange chromatography on carboxymethyl cellulose and polyacrylamide gel electrophoresis. The protein components of the dimers were identified electrophoretically after periodate cleavage of the cross-link(s). The analysis revealed 42 cross-linked dimers involving 25 different proteins. Among these, proteins S3, S4 and S20 occurred in combinations with six, eight and seven different proteins, respectively. For proteins S13, S14 and S17 five protein neighbours could be identified, while 13 of the remaining proteins were linked to three or four different protein partners. The involvement of the majority of proteins in the formation of multiple cross-linked dimers implies that a large number of protein-protein interaction sites exist within the ribosomal subunit. A preliminary model illustrating the arrangement of 16 proteins in the small ribosomal subunit is presented and discussed with respect to possible functions, especially in the event of translation initiation.
Gross
B
B
Central Institute of Molecular Biology, Department of Cell Physiology, Academy of Sciences of GDR, Berlin-Buch.
Westermann
P
P
Bielka
H
H
D000818:Animals; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007096:Imidoesters; D008099:Liver / Q000737:chemistry*; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000737:chemistry*
D003432:Cross-Linking Reagents; D007096:Imidoesters; D012269:Ribosomal Proteins; C030506:dimethyl-3,8-diaza-4,7-dioxo-5,6-dihydroxydecanbis(imidate)