PCR technique for identification of mussel species.
Random amplified polymorphic DNA (RAPD) analysis has been applied to the identification of four mussels species: Mytilus edulis, Mytilus chilensis, Mytilus galloprovincialis, and Perna canaliculus. Amplifications of DNA from mussel were carried out using random primers. The most distinctive bands were then isolated, cloned, and sequenced to design specific primers. Finally, DNA from different mussels was amplified with these specific primers, and results allow genetic identification of M. galloprovincialis from the rest of the mussel species.
Rego
Ignacio
I
Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain.
Martínez
Andrés
A
González-Tizón
Ana
A
Vieites
Juan
J
Leira
Francisco
F
Méndez
Josefina
J
D000818:Animals; D049872:Bivalvia / Q000145:classification* / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011786:Quality Control; D015203:Reproducibility of Results; D017747:Seafood
D017931:DNA Primers; D004247:DNA