Significance of runaway calcium homeostasis in cultured cardiomyocytes in development of hypoxia, burnt serum-induced injury.
OBJECTIVE: This study aimed at understanding of the role of calcium homeostasis in cardiomyocytes from hypoxia, burnt serum-induced injury. METHODS: Alterations in cytosolic free calcium concentration (Ca(i)), calcium influx and viability of the cardiomyocytes in vitro after hypoxia, burnt serum stimulus were observed. RESULTS: Ca(i) increased markedly, in the meantime, the cellular transmembrane calcium influx increased and the viability of the cells decreased significantly following hypoxia, burnt serum-induced injury. CONCLUSIONS: In our study cytosolic calcium ion was transported abnormally in the cardiomyocytes after burn, to result in Ca(i) increase and runaway calcium homeostasis, thus the normal cellular function was disturbed. This may be one of the important factors in the development of burn-induced cardiac injury.
Chi
Luxiang
L
Burn Research Institute, Southwestern Hospital, Third Military Medical College, Chongqing 400038, China.
Yang
Zhongcheng
Z
Wang
Xu
X
Li
Ao
A