[Teratogenic action of antiepileptic drug: 2-propylpentanoic acid-effects on rat and hamster embryos cultured in vitro].
Valproic acid (2-propylpentanoic acid) has become accepted as one of the most important antiepileptic drug. Although clinically effective, this drug remains controversial as suspected of being a human teratogen. The aim of our study was to test the embryotoxic potential of valproic acid using whole embryo culture assay. For that purpose rat and hamster embryos were explanted on gestation day 9 and 7 respectively, and cultured for 48 and 24 hours. During the culture period embryos were exposed to valproic acid (VPA) at a concentrations of: 10, 50 and 100 mg/ml. In our study this drug exhibited dose-related embryotoxic and teratogenic effects in rat and hamster embryos. Analysis of the results indicated that hamster embryos were more susceptible to VPA than rat embryos.
Włodarczyk
B
B
Zakładu Farmakologii i Toksykologii Państwowego Instytutu Weterynaryjnego w Puławach.
Biernacki
B
B
Minta
M
M
D000014:Abnormalities, Drug-Induced / Q000209:etiology*; D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000002:abnormalities* / Q000187:drug effects*; D005260:Female; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014635:Valproic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*
D000927:Anticonvulsants; D014635:Valproic Acid