title: Identification of novel E2F1-regulated genes by microarray.
abstract: The E2F pathway has been proposed to regulate genes involved in the transition from quiescence into DNA synthesis. However, this hypothesis has not been rigorously tested on a genomic scale. Toward this end, we have infected quiescent mouse fibroblasts, which do not express E2F1, with an E2F1-expressing adenovirus and examined the expression of more than 6000 genes using high-density microarrays. Microarray results clearly support the current paradigm; however, they suggest that E2F1 may also regulate unanticipated cellular functions including pathways involved in apoptosis, signal transduction, transcriptional control, and membrane biology. Most surprisingly, we identified a number of genes that are repressed by E2F1 expression, suggesting that E2F1 may have the potential to repress transcription of numerous genes through an unknown mechanism.
authors.affiliation: Program in Molecular Oncology, H. Lee Moffitt Comprehensive Cancer Center and Research Institute, University of South Florida, Tampa, 33612, USA.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Ma
authors.forename: Rhonda
authors.initials: R
authors.lastname: Croxton
authors.forename: Ronnie L
authors.initials: RL
authors.lastname: Moorer
authors.forename: W Douglas
authors.initials: WD
authors.lastname: Cress
mesh_terms: D016475:3T3 Cells; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D020868:Gene Silencing; D005822:Genetic Vectors; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050687:E2F1 Transcription Factor; C493754:E2f1 protein, mouse; D014157:Transcription Factors