[Decolorization and isolation of recombinant hirudin expressed in the methylotrophic yeast Pichia pastoris].
Schemes for decolorization of broth and isolation of recombinant hirudin from the fermentation supernatants of recombinant yeast Pichia pastoris were developed to remove a great deal of non-desired proteins and coloring matters. Based on the stability of hirudin, heating is desirable to pre-treat the fermentation broth. Several different column chromatographies, including anion-exchange, cation-exchange, hydrokyapatite adsorption, hydrophobic interaction, gel filtration and their combination were investigated on the efficiencies of decolorization and isolation. Among these methods, the combination of cation-exchange and anion-exchange was found to be a success in both decolorization and isolation of hirudin.
Zhou
W B
WB
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Zhou
X S
XS
Zhang
Y X
YX
D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005285:Fermentation / Q000502:physiology; D006629:Hirudins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D006358:Hot Temperature; D010843:Pichia / Q000235:genetics*; D011480:Protease Inhibitors / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification
D006629:Hirudins; D011480:Protease Inhibitors; D011994:Recombinant Proteins