Chlamydia pneumoniae DNA in peripheral venous blood samples from patients with carotid artery stenosis.
In the study presented here, peripheral blood specimens obtained from patients with atherosclerosis were examined for the presence of Chlamydia pneumoniae to determine whether these specimens can be used for routine testing. Chlamydia pneumoniae DNA was detected in 7 of 56 patients with carotid stenosis and in three of four patients with other atherosclerotic diseases, but it was not detected in any of 50 healthy controls or in any of 59 age- and gender-matched patients suffering from other nonatherosclerotic diseases. IgG antibodies indicative of an active Chlamydia pneumoniae infection were detected by microimmunofluorescence in two of nine PCR-positive patients but in none of 41 PCR-negative patients. Four of nine serum samples obtained from PCR-positive patients contained IgA antibodies compared to 5 of 41 samples obtained from PCR-negative patients.
Freidank
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HM
Abteilung Mikrobiologie und Hygiene, Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, Germany. friedank@ukl.uni-freiburg.de
Lux
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Dern
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Meyer-König
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U
Els
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T
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D016893:Carotid Stenosis / Q000097:blood / Q000453:epidemiology / Q000382:microbiology*; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D016491:Peripheral Vascular Diseases / Q000097:blood / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012016:Reference Values; D012307:Risk Factors; D012494:Sampling Studies; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial