Mimicking a cytoskeleton by coupling poly(N-isopropylacrylamide) to the inner leaflet of liposomal membranes: effects of photopolymerization on vesicle shape and polymer architecture.
Networks of N-isopropylacrylamide (NIPAM) copolymers, coupled to spherical phospholipid bilayers, are suitable as a model for the study of the interaction between the cytoskeleton and cellular membranes, as well as for promising new drug delivery systems with triggerable drug release properties and improved stability. In this article, we describe a simple preparation technique for liposomes from egg phosphatidyl choline (EPC) encapsulating a cross-linked NIPAMminus signTEGDM copolymer skeleton (tetraethylene glycol dimethacrylate, TEGDM) which is coupled only to the inner monolayer by a novel membrane anchor monomer. Polymerization in the lipid vesicles was initiated at the inner membrane surface by the radical initiator 2,2-diethoxy-acetophenone (DEAP) permeating through the membrane from the outside. The effects of photopolymerization and polymer formation on vesicle shape and membrane integrity were studied by transmission electron microscopy (TEM), cryo-TEM, and atomic force microscopy (AFM). Upon UV irradiation, approximately 100% of the vesicles contained a polymer gel and only occasional changes in the spherical shape of the liposomes were observed. The architecture of the polymer network inside the liposomal compartment was determined by the conditions of the photopolymerization. Composite structures of polymer hollow spheres or solid spheres, respectively, tethered to spherical membrane vesicles were produced. The increased stability of the polymer-tethered lipid bilayers against solubilization by sodium cholate, compared to pure EPC vesicles, was determined by radiolabeling the lipid membrane.
Stauch
Oliver
O
Department of Pharmaceutical Technology, Hermann-Herder-Strasse 9, Albert-Ludwigs-Universität Freiburg, D-79104 Freiburg im Breisgau, Germany. stauch@uni-freiburg.de
Uhlmann
Thomas
T
Fröhlich
Margret
M
Thomann
Ralf
R
El-Badry
Mahmoud
M
Kim
Yong-Keun
YK
Schubert
Rolf
R
D000180:Acrylic Resins / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D020285:Cryoelectron Microscopy; D003599:Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D010777:Photochemistry; D014466:Ultraviolet Rays
D000180:Acrylic Resins; D008081:Liposomes; C052970:poly-N-isopropylacrylamide