title: An integrated physical and genetic map of the rice genome.
abstract: Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome fingerprint-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome ( approximately 90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes.
authors.affiliation: Clemson University Genomics Institute, 100 Jordan Hall, Clemson, South Carolina 29634-5727, USA.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Chen
authors.forename: Gernot
authors.initials: G
authors.lastname: Presting
authors.forename: W Brad
authors.initials: WB
authors.lastname: Barbazuk
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Goicoechea
authors.forename: Barbara
authors.initials: B
authors.lastname: Blackmon
authors.forename: Guangchen
authors.initials: G
authors.lastname: Fang
authors.forename: Hyeran
authors.initials: H
authors.lastname: Kim
authors.forename: David
authors.initials: D
authors.lastname: Frisch
authors.forename: Yeisoo
authors.initials: Y
authors.lastname: Yu
authors.forename: Shouhong
authors.initials: S
authors.lastname: Sun
authors.forename: Stephanie
authors.initials: S
authors.lastname: Higingbottom
authors.forename: John
authors.initials: J
authors.lastname: Phimphilai
authors.forename: Dao
authors.initials: D
authors.lastname: Phimphilai
authors.forename: Scheen
authors.initials: S
authors.lastname: Thurmond
authors.forename: Brian
authors.initials: B
authors.lastname: Gaudette
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Jingdong
authors.initials: J
authors.lastname: Liu
authors.forename: Jamie
authors.initials: J
authors.lastname: Hatfield
authors.forename: Dorrie
authors.initials: D
authors.lastname: Main
authors.forename: Kasey
authors.initials: K
authors.lastname: Farrar
authors.forename: Caroline
authors.initials: C
authors.lastname: Henderson
authors.forename: Laura
authors.initials: L
authors.lastname: Barnett
authors.forename: Ravi
authors.initials: R
authors.lastname: Costa
authors.forename: Brian
authors.initials: B
authors.lastname: Williams
authors.forename: Suzanne
authors.initials: S
authors.lastname: Walser
authors.forename: Michael
authors.initials: M
authors.lastname: Atkins
authors.forename: Caroline
authors.initials: C
authors.lastname: Hall
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Budiman
authors.forename: Jeffery P
authors.initials: JP
authors.lastname: Tomkins
authors.forename: Meizhong
authors.initials: M
authors.lastname: Luo
authors.forename: Ian
authors.initials: I
authors.lastname: Bancroft
authors.forename: Jerome
authors.initials: J
authors.lastname: Salse
authors.forename: Farid
authors.initials: F
authors.lastname: Regad
authors.forename: Trilochan
authors.initials: T
authors.lastname: Mohapatra
authors.forename: Nagendra K
authors.initials: NK
authors.lastname: Singh
authors.forename: Akhilesh K
authors.initials: AK
authors.lastname: Tyagi
authors.forename: Carol
authors.initials: C
authors.lastname: Soderlund
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Dean
authors.forename: Rod A
authors.initials: RA
authors.lastname: Wing
mesh_terms: D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D019295:Computational Biology; D020451:Contig Mapping / Q000379:methods; D020732:Cytogenetic Analysis; D016172:DNA Fingerprinting; D015723:Gene Library; D005819:Genetic Markers; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers