Influence of equine herpesvirus type 2 infection on monocyte chemoattractant protein 1 gene transcription in equine blood mononuclear cells.
Representational difference analysis (RDA) was used to compare gene expression in equine mononuclear cells either infected with equine herpesvirus-2 (EHV-2) or adsorbed with inactivated EHV-2. Seven clones identified in non-infected cells after three rounds of selective subtraction and enrichment for differentially expressed genes contained sequences homologous to equine monocyte chemoattractant protein 1 (MCP-1). This suggested that EHV-2 may down-regulate MCP-1 transcription in infected cells. These findings correlate well with similar findings described for human cytomegalovirus and support the view that EHV-2 may have the ability to modify the chemokine environment of infected cells. This may constitute an important feature of EHV-2 biology, because such an ability has the potential to compromise host defence mechanisms and predispose to infection with other pathogens.
Dunowska
M
M
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand. mdunowsk@lamar.colostate.edu
Meers
J
J
Johnson
R D
RD
Wilks
C R
CR
D000818:Animals; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D015536:Down-Regulation; D006566:Herpesviridae Infections / Q000097:blood / Q000662:veterinary* / Q000821:virology; D006734:Horse Diseases / Q000097:blood* / Q000821:virology*; D006736:Horses; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000821:virology*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D018144:Rhadinovirus / Q000502:physiology*; D014158:Transcription, Genetic
D018932:Chemokine CCL2; D012333:RNA, Messenger