Comparison of the immune responses induced by local immunizations with recombinant Lactobacillus plantarum producing tetanus toxin fragment C in different cellular locations.
Lactobacillus plantarum NCIMB8826 was selected as a bacterial carrier for the development of live mucosal vaccines. This strain was reported to display interesting pharmaco-kinetic properties when fed to human volunteers and is also able to persist in the mouse intestine. The non-toxic C fragment of tetanus toxin (TTFC) was used as a model antigen. Recombinant strains producing TTFC in three cellular locations, intracellular, secreted or cell-surface exposed were compared to each other by immunizing mice through the subcutaneous, intranasal and intragastric routes. The three types of constructs were able to induce strong specific immune responses against TTFC by all routes tested. While cell-surface presentation required lower antigen doses to be immunogenic, the highest IgG serum antibody titers were obtained with the strain producing large amounts of TTFC in the cytoplasm.
Reveneau
Nathalie
N
Laboratoire de Bactériologie des Ecosystèmes, INSERM IFR17, Institut Pasteur de Lille, 1 rue du Professeur Calmette, F-59019 Lille Cedex, France.
Geoffroy
Marie Claude
MC
Locht
Camille
C
Chagnaud
Patrice
P
Mercenier
Annick
A
D000281:Administration, Intranasal; D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001483:Base Sequence; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal; D007074:Immunoglobulin G / Q000096:biosynthesis; D007279:Injections, Subcutaneous; D007700:Kinetics; D007778:Lactobacillus / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D013744:Tetanus Toxin / Q000235:genetics* / Q000276:immunology*; D013745:Tetanus Toxoid / Q000008:administration & dosage* / Q000235:genetics; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D010446:Peptide Fragments; D013744:Tetanus Toxin; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; C040635:tetanus toxin fragment C