Viral gene expression during acute simian varicella virus infection.
Simian varicella virus (SVV) causes a natural varicella-like disease in nonhuman primates. Outbreaks of simian varicella occur sporadically in primate facilities. Simian varicella is used as a model for investigation of varicella-zoster virus (VZV) pathogenesis and latency. In this study, SVV gene expression and histopathology were analysed in tissues of acutely infected vervet monkeys. RT-PCR analysis demonstrated expression of specific SVV immediate early, early and late genes in the skin, lung, liver and ganglia tissues of acutely infected monkeys. Viral antigen expression and histopathology, including necrosis and inflammation, were detected in the skin, lungs, liver and spleen of infected monkeys by immunohistochemical analysis. Viral antigen expression, but little or no histopathology, was evident in the neural ganglia, the eventual site of viral latency. The study provides a foundation for further investigation on the role of viral genes in varicella pathogenesis and latency.
Gray
Wayne L
WL
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham St, Little Rock, Arkansas 72205, USA1.
Mullis
Lisa
L
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, 4301 W. Markham St, Little Rock, Arkansas 72205, USA1.
Soike
Kenneth F
KF
Tulane University Regional Primate Center, Covington, LA 70433, USA2.
D000208:Acute Disease; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002644:Chickenpox / Q000821:virology*; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000821:virology*; D006568:Herpesvirus 1, Cercopithecine / Q000235:genetics* / Q000276:immunology; D007150:Immunohistochemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000956:Antigens, Viral; D004279:DNA, Viral