Fabrication and characterization of a rigid magnetic matrix for protein adsorption.
This article describes the fabrication and characterization of a novel magnetic poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) matrix containing magnetite colloids. The results showed that the matrix was superparamagnetic and could be separated magnetically from a suspension in a few seconds. Protein adsorption properties of diethylamine-derivatized matrix were characterized with bovine serum albumin (BSA) as a model protein. The static capacity determined by batch adsorption was 79 mg/ml wet matrix. Kinetic study gave an effective diffusivity of BSA of 5.0 x 10(-13) m2/s in the matrix at an initial BSA concentration in the liquid phase of 1.0 mg/ml. Stability of the matrix was confirmed by recycling of the matrix in protein adsorptions.
Xue
Bo
B
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, China.
Sun
Yan
Y
D000327:Adsorption; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D012710:Serum Albumin, Bovine