Detection of biogenic CO production above vascular cell cultures using a near-room-temperature QC-DFB laser.
We report the first application of pulsed, near-room-temperature quantum cascade laser technology to the continuous detection of biogenic CO production rates above viable cultures of vascular smooth muscle cells. A computer-controlled sequence of measurements over a 9-h period was obtained, resulting in a minimum detectable CO production of 20 ppb in a 1-m optical path above a standard cell-culture flask. Data-processing procedures for real-time monitoring of both biogenic and ambient atmospheric CO concentrations are described.
Kosterev
A A
AA
Rice Quantum Institute, Rice University, Houston, TX 77251-1892, USA. akoster@rice.edu
Tittel
F K
FK
Durante
W
W
Allen
M
M
Kohler
R
R
Gmachl
C
C
Capasso
F
F
Sivco
D L
DL
Cho
A Y
AY
D000389:Air Conditioning; D000818:Animals; D002248:Carbon Monoxide / Q000032:analysis* / Q000378:metabolism*; D002478:Cells, Cultured; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D007834:Lasers; D008021:Life Support Systems / Q000295:instrumentation; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012815:Signal Processing, Computer-Assisted; D013055:Spectrophotometry, Infrared; D013696:Temperature
D002248:Carbon Monoxide