title: Detection and identification of the Candida species by 25S ribosomal DNA analysis in the urine of candidal cystitis.
abstract: Candida species in clinical urine samples were identified directly by the newly developed method of PCR analysis on 25S ribosomal DNA (rDNA). Two dogs were referred to the Animal Medical Center, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan for the examination of chronic cystitis. Microscopic examination of urine samples from these dogs revealed yeast cells. Urine culture on Sabouraud's dextrose agar at 27 degrees C for 5 days produced white to cream colored colonies. The isolates were identifical to Candida albicans and C. parapsilosis by mycological examination, respectively. The nucleotide sequences of 25S ribosomal DNA from these urine isolates showed 99% similarity to those of a reference strain of Candida albicans or C. parapsilosis. The nucleotide sequences of 25S rDNA obtained directly from urine samples were also identical to C. albicans and C. parapsilosis, respectively. Confirming the results on the isolates cultured from the same urine samples. This PCR analysis method could be available for the direct identification of Candida species in urine samples within 2 days.
authors.affiliation: Department of Pathobiology, Nihon University, School of Veterinary Medicine, Fujisawa, Kangawa, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Kano
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Hattori
authors.forename: Katsuko
authors.initials: K
authors.lastname: Okuzumi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Rie
authors.initials: R
authors.lastname: Yamauchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koie
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Watari
authors.forename: Atsuhiko
authors.initials: A
authors.lastname: Hasegawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002176:Candida albicans / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis / Q000652:urine / Q000662:veterinary*; D003556:Cystitis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D004271:DNA, Fungal / Q000652:urine*; D004275:DNA, Ribosomal / Q000652:urine*; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D004285:Dogs; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000235:genetics; D012680:Sensitivity and Specificity; D017385:Sequence Homology; D014556:Urine / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C065039:RNA, ribosomal, 25S