title: Simultaneous expression of mu- and gamma-chain mRNA in cloned murine B-lymphoma cell lines.
abstract: The expression of immunoglobulin heavy chain genes was studied in five murine B-lymphomas known from previous studies to express either mu (38C-13), mu + delta (L10A, K46, BCL1) or gamma chains (A20). The presence of mu- and gamma-mRNAs in these tumors was determined by Northern blot analyses of the total cell poly(A)+ mRNA, using the appropriate 32P-labeled recombinant plasmid probes. In four out of the five lymphomas examined, both mu- and gamma-mRNAs were detected. The mu-mRNA appeared as multiple discrete bands of 1.9-3.0 kb. In three out of the four lymphomas, the gamma-mRNA appeared as two bands, a major one of 1.9 and a minor one of 3.9 kb. Three myelomas examined by similar methods did not contain more than one class of heavy chain mRNA. Reexamination of the Ig chains produced by the B-lymphomas which expressed both mu- and gamma-mRNAs revealed that two of them preserved their original phenotype and expressed mu (38C-13) or gamma chains only (A20). In contrast, two of the cell lines previously shown to express mu but not gamma chains (i.e., L10A and K46R) had changed during growth in culture and 'switched' to the production of gamma chains only. These results indicate that, in contrast to myelomas, B-lymphomas possess two classes of mRNA. However, the production of heavy chain mRNA in B-lymphomas is not necessarily accompanied by synthesis of the corresponding polypeptide chains. More studies are necessary to find out whether the expression of 'non-productive' heavy chain mRNA molecules in B-lymphomas is related to the phenomena of 'allelic exclusion' and/or the 'heavy chain switch' which occurs during the maturation of B-cells.
authors.affiliation: The Hubert H.Humphrey Centre for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Laskov
authors.forename: R
authors.initials: R
authors.lastname: Ishay-Michaeli
authors.forename: M
authors.initials: M
authors.lastname: Wallach
authors.forename: D
authors.initials: D
authors.lastname: Givol
authors.forename: K J
authors.initials: KJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D016393:Lymphoma, B-Cell / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm