First description of Klebsiella pneumoniae harboring CTX-M beta-lactamases (CTX-M-14 and CTX-M-3) in Taiwan.
Klebsiella pneumoniae isolates from Taiwan medical centers (50 strains; 1998 to 2000) with a CTX-M resistance phenotype (ceftazidime susceptible and ceftriaxone or cefotaxime nonsusceptible) were selected for initial isoelectric focusing analysis. beta-Lactamases with pIs of 7.9 (n = 22) and 8.4 (n = 28) in addition to 5.4 and/or 7.6 were detected. DNA gene sequencing identified the beta-lactamases with pIs of 7.9 and 8.4 as CTX-M-14 and CTX-M-3, respectively. Molecular typing suggested inter- and intrahospital clonal dissemination of these Taiwanese CTX-M-producing Klebsiella strains.
Yu
Wen Liang
WL
University of Iowa College of Medicine, Iowa City, USA. .
Winokur
Patricia L
PL
Von Stein
Diana L
DL
Pfaller
Michael A
MA
Wang
Jen Hsien
JH
Jones
Ronald N
RN
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan / Q000453:epidemiology; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism*
D004269:DNA, Bacterial; C456080:beta-lactamase CTX-M-14; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases