title: Molecular "wiring" enzymes in organized nanostructures.
abstract: We report on the "molecular wiring" efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium-derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have demonstrated that the specific rate of bimolecular FADH(2) oxidation ("wiring efficiency") is limited by the diffusion-like electron hopping mechanism in the multilayers.
authors.affiliation: INQUIMAE-Departamento de Química Inorgánica, Analítica y Química Físca, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, AR-1428 Buenos Aires, Argentina.
authors.forename: Ernesto J
authors.initials: EJ
authors.lastname: Calvo
authors.forename: Claudia
authors.initials: C
authors.lastname: Danilowicz
authors.forename: Alejandro
authors.initials: A
authors.lastname: Wolosiuk
mesh_terms: D004563:Electrochemistry; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D036103:Nanotechnology / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D009992:Osmium / Q000737:chemistry*; D010084:Oxidation-Reduction; D011725:Pyridines / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D011725:Pyridines; D009992:Osmium; D005949:Glucose Oxidase; D005947:Glucose