Effect of T-2 toxin on in vivo lipid peroxidation and vitamin E status in mice.
The effects of an acute administration of T-2 toxin on vitamin E status and the corresponding degree of lipid peroxidation, as determined by the plasma and organ content of malondialdehyde (MDA), was studied in mice. The effects of T-2 toxin administration on the body weight and weights of liver, spleen and thymus were also assessed. T-2 toxin was administered in doses ranging from 1 to 6.25 mg/kg body weight, depending on the experiment, while the dietary content of vitamin E ranged from near 0 to 5000 IU/kg. There was a significant decrease in vitamin E content of plasma after the administration of the toxin with the concentrations remaining low for periods as long as 48-72 h. MDA content of liver increased significantly after 24-48 h of toxin administration in contrast to the controls. However, MDA levels returned to the control range after 72 h. The concentrations of MDA in liver were inversely related to the vitamin E content of the diet, and were always higher for the toxin-treated animals (significant linear regression between MDA content of liver and the log10 of vitamin E content of the diet). Weights of spleen and thymus decreased after T-2 toxin administration; however, the weight of liver either increased or did not change in the different experiments. In conclusion, T-2 toxin treatment of mice increased lipid peroxidation in the liver as measured by MDA production. This process was maximal after 48 h of T-2 challenge, and decreased thereafter. Plasma alpha-tocopherol levels decreased as soon as 6 h after the toxin challenge, while MDA did not increase until there was a severe depletion of vitamin E. These changes were accompanied by decrease in weight of spleen and thymus.
Vilà
B
B
Department of Animal Science, University of Manitoba, Winnipeg, R3T 2N2, Manitoba, Canada.
Jaradat
Z W
ZW
Marquardt
R R
RR
Frohlich
A A
AA
D000284:Administration, Oral; D000818:Animals; D001835:Body Weight; D004305:Dose-Response Relationship, Drug; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000473:pathology; D051379:Mice; D013154:Spleen / Q000473:pathology; D013605:T-2 Toxin / Q000009:adverse effects*; D013950:Thymus Gland / Q000473:pathology; D014810:Vitamin E / Q000097:blood / Q000378:metabolism
D014810:Vitamin E; D013605:T-2 Toxin