Effect of fumonisin B1 on structure and function of macrophage plasma membrane.
Fumonisin B1 (FB1), a mycotoxin produced by Fusarium moniliforme and related fungi, is nephrotoxic, neurotoxic, hepatotoxic, carcinogenic and immunosuppressive in animals and man. In this study we evaluate the modifications of fluidity, endocytosis and peroxidative damage of plasma membrane induced by FB1 in macrophage cell line J774A.1. In these immune cells FB1 (1-10 microM) enhances membrane fluidity and increases, time-dependently, the horseradish peroxidase (HRP) endocytosis. This effect is concentration-dependent, significant at 10 microM, and reverted by IFN-gamma (100 U/ml). Moreover, FB1 (1-10 microM) induces a membrane peroxidative damage as evident by the increase of malondialdehyde (MDA) production. All these mycotoxin effects provide additional insight into potential mechanism by which FB1, in macrophages, might enhance membrane damage and oxidative stress contributing to the pathogenesis of mycotoxin induced diseases.
Ferrante
Maria C
MC
Department of Pathology and Animal Health, University of Naples Federico II, Via Delpino 1, 80137, Naples, Italy.
Meli
Rosaria
R
Mattace Raso
Giuseppina
G
Esposito
Emanuela
E
Severino
Lorella
L
Di Carlo
Giulia
G
Lucisano
Antonia
A
D000818:Animals; D002264:Carboxylic Acids / Q000633:toxicity*; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D004705:Endocytosis / Q000187:drug effects; D037341:Fumonisins; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D007371:Interferon-gamma / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D009183:Mycotoxins / Q000633:toxicity*; D018384:Oxidative Stress
D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1; D008315:Malondialdehyde; D007371:Interferon-gamma; D006735:Horseradish Peroxidase