Synthesis of polyhydroxyalkanoate in the peroxisome of Pichia pastoris.
Polyhydroxyalkanoates (PHAs) are polyesters naturally produced by bacteria that have properties of biodegradable plastics and elastomers. A PHA synthase from Pseudomonas aeruginosa modified at the carboxy-end for peroxisomal targeting was transformed in Pichia pastoris. The PHA synthase was expressed under the control of the promoter of the P. pastoris acyl-CoA oxidase gene. Synthesis of up to 1% medium-chain-length PHA per g dry weight was dependent on both the expression of the PHA synthase and the presence of oleic acid in the medium. PHA accumulated as inclusions within the peroxisomes. P. pastoris could be used as a model system to study how peroxisomal metabolism needs to be modified to increase PHA production in other eukaryotes, such as plants.
Poirier
Yves
Y
Laboratoire de Biotechnologie Végétale, Institut d'Ecologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. yves.poirier@ie-bpv.unil.ch
Erard
Nadine
N
MacDonald-Comber Petétot
Jean
J
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D008854:Microscopy, Electron; D020675:Peroxisomes / Q000378:metabolism* / Q000648:ultrastructure; D010843:Pichia / Q000201:enzymology* / Q000235:genetics*; D011091:Polyesters / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics; D011995:Recombination, Genetic
D003470:Culture Media; D011091:Polyesters; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase