title: Opioid signalling in the rat rostral ventrolateral medulla.
abstract: 1. The present article reviews several aspects of opioid signalling in the rostral ventrolateral medulla (RVLM) and their implications for the neural control of blood pressure. 2. In the RVLM, preproenkephalin (PPE) mRNA is expressed by bulbospinal cells that are strongly barosensitive. These putative presympathetic neurons includes C1 and non-C1 neurons. 3. In the RVLM, PPE mRNA is also present in GABAergic neurons that do not project to the thoracic spinal cord. 4. Rostral ventrolateral medulla presympathetic cells receive enkephalinergic inputs and express mu-opioid receptors (MOR). Some of their synaptic inputs also contain MOR. 5. Pre- and post-synaptic modulation of RVLM presympathetic neurons by MOR agonists has been demonstrated in slices of neonate brain. The post-synaptic effect is inhibitory (increased gK). Presynaptic effects include disfacilitation (reduction of glutamate release) and possibly dishinhibition (reduction of GABA release). 6. In conclusion, opioid signalling plays a pervasive role in the medullospinal network that controls sympathetic tone and arterial pressure. Opioid peptides are made by the presympathetic, presumably excitatory, cells of the RVLM and by local GABAergic inhibitory neurons. In addition, RVLM presympathetic neurons are also controlled by opioid peptides at the pre- and post-synaptic level. mu-Opioid receptors are found post-synaptically, whereas presynaptic receptors probably include both mu and delta subtypes. Conditions that trigger the release of opioid peptides by presympathetic neurons or by inputs to these cells are not fully understood and may include decompensated haemorrhage and certain types of peripheral sensory stimulation related to acupuncture.
authors.affiliation: Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, Virginia, USA. pgg@virginia.edu
authors.forename: Patrice G
authors.initials: PG
authors.lastname: Guyenet
authors.forename: Ruth L
authors.initials: RL
authors.lastname: Stornetta
authors.forename: Ann M
authors.initials: AM
authors.lastname: Schreihofer
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Pelaez
authors.forename: Abdallah
authors.initials: A
authors.lastname: Hayar
authors.forename: Sue
authors.initials: S
authors.lastname: Aicher
authors.forename: Ida J
authors.initials: IJ
authors.lastname: Llewellyn-Smith
mesh_terms: D000818:Animals; D004745:Enkephalins / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D008526:Medulla Oblongata / Q000187:drug effects / Q000502:physiology*; D009292:Narcotic Antagonists; D009294:Narcotics / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D011498:Protein Precursors / Q000494:pharmacology / Q000502:physiology; D051381:Rats; D011957:Receptors, Opioid / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology; D014666:Vasomotor System / Q000187:drug effects / Q000502:physiology
chemical_list: D004745:Enkephalins; D009292:Narcotic Antagonists; D009294:Narcotics; D011498:Protein Precursors; D011957:Receptors, Opioid; C033660:preproenkephalin