Value of flow cytometric assay for the detection of antisperm antibodies in women with a history of recurrent abortion.
OBJECTIVE
To verify the proposed relationship between recurrent spontaneous abortions and the presence of maternal antisperm antibodies (ASA) in women as detected by a sensitive and reliable method.


METHODS
The presence of maternal antipaternal immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies were determined against three different paternal antigens comprising T, B lymphocytes and semen cells by a sensitive flow cytometric crossmatch method to examine their possible correlation with pregnancy outcome. Group 1 consisted of sera obtained from 24 women with a history of abortion, and lymphocytes and semen samples collected from their husbands at the same time of visiting the in vitro fertilization (IVF) Clinic at King Faisal Specialist Hospital and Research Center. Sera, lymphocytes and semen samples were also collected from six couples with no history of abortion who served as controls (Group 2).


RESULTS
Using a sensitive flow cytometric assay to analyse the samples, without knowledge of clinical status, elevated levels of both IgG and IgM were detected in Group 1. However, no significant association was found when compared with normal females who had healthy pregnancies.


CONCLUSIONS
Flow cytometry is a highly sensitive and specific tool for the detection of alloantibodies in human sera from patients with rejected transplanted organs. Our findings suggest that maternal antipaternal antibodies with respect to IgG and IgM classes do not play a major role in women with a history of recurrent abortions, despite the presence of increased levels of antibodies against three different sources of paternal antigens.
Al-Hussein
Khaled
K
Department of Biological and Medical Research, King Faisal Specialist and Research Center, Riyadh, Saudi Arabia. khussein@kfshrc.edu.sa
Al-Mukhalafi
Zuha
Z
Bertilsson
Per-Anders
PA
Jaroudi
Kamal
K
Shoukri
Mohammed
M
Hollander
Johanson
J
D000026:Abortion, Habitual / Q000276:immunology*; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007518:Isoantibodies / Q000097:blood*; D008297:Male; D011247:Pregnancy; D012680:Sensitivity and Specificity; D013094:Spermatozoa / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies