title: Expression of winged bean basic agglutinin in Spodoptera frugiperda insect cell expression system.
abstract: In this paper we report the successful expression of the winged bean basic agglutinin (WBA I) in insect cells infected with a recombinant baculovirus carrying the WBA I gene and its characterization in terms of its carbohydrate binding properties. The expressed protein appears to have a lower molecular weight than the native counterpart which is consistent with the lack of glycosylation of the former. Moreover, the expressed protein maintains its dimeric nature. Hence, a role for glycosylation in modulation of dimerization of WBA I is ruled out unlike Erythrina corallodendron (EcorL). Despite this the protein is active, with its sugar specificity unaltered.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
authors.forename: V R
authors.initials: VR
authors.lastname: Srinivas
authors.forename: K
authors.initials: K
authors.lastname: Bachhawat-Sikder
authors.forename: S
authors.initials: S
authors.lastname: Habib
authors.forename: S E
authors.initials: SE
authors.lastname: Hasnain
authors.forename: A
authors.initials: A
authors.lastname: Surolia
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D050260:Carbohydrate Metabolism; D002478:Cells, Cultured; D019281:Dimerization; D005057:Eukaryotic Cells / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D006031:Glycosylation; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D011401:Promoter Regions, Genetic / Q000502:physiology; D011485:Protein Binding / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D018411:Spodoptera / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D037102:Lectins; D037121:Plant Lectins; D011993:Recombinant Fusion Proteins; C049304:winged bean lectin