Caspase 3 regulates phosphatidylserine externalization and phagocytosis of oxidatively stressed erythrocytes.
The appearance of phosphatidylserine (PS) on the outer surface of red cells is an important signal for their uptake by macrophages. We report for the first time that procaspase 3 present in the anucleated mature human erythrocyte is activated under oxidative stress induced by t-butylhydroperoxide leading to impairment of the aminophospholipid translocase, PS externalization and increased erythrophagocytosis. This is the first report linking caspase 3 activation to inhibition of flippase activity and uptake of red cells by macrophages.
Mandal
Debabrata
D
Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra Road, 700009, Kolkata, India.
Moitra
Prasun K
PK
Saha
Samiran
S
Basu
Joyoti
J
D002352:Carrier Proteins / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000502:physiology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D018384:Oxidative Stress / Q000502:physiology*; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D010718:Phosphatidylserines / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D002352:Carrier Proteins; D015853:Cysteine Proteinase Inhibitors; D008565:Membrane Proteins; D009842:Oligopeptides; D010718:Phosphatidylserines; D049294:Phospholipid Transfer Proteins; C110772:benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone; D020122:tert-Butylhydroperoxide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases