title: Association of the human SUMO-1 protease SENP2 with the nuclear pore.
abstract: SUMO-1 is a small ubiquitin-like protein that can be covalently conjugated to other proteins. A family of proteases catalyzes deconjugation of SUMO-1-containing species. Members of this family also process newly synthesized SUMO-1 into its conjugatable form. To understand these enzymes better, we have examined the localization and behavior of the human SUMO-1 protease SENP2. Here we have shown that SENP2 associates with the nuclear face of nuclear pores and that this association requires protein sequences near the N terminus of SENP2. We have also shown that SENP2 binds to Nup153, a nucleoporin that is localized to the nucleoplasmic face of the pore. Nup153 binding requires the same domain of SENP2 that mediates its targeting in vivo. Removal of the Nup153-interacting region of SENP2 results in a significant change in the spectrum of SUMO-1 conjugates within the cell. Our results suggest that association with the pore plays an important negative role in the regulation of SENP2, perhaps by restricting its activity to a subset of the conjugated proteins within the nucleus.
authors.affiliation: Laboratory of Gene Regulation and Development, NICHD, National Institutes of Health, Bethesda, Maryland 20892-5431, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Hang
authors.forename: Mary
authors.initials: M
authors.lastname: Dasso
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002467:Cell Nucleus / Q000201:enzymology* / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D025842:SUMO-1 Protein / Q000737:chemistry* / Q000378:metabolism*; D014981:Xenopus
chemical_list: D018076:DNA, Complementary; C079377:NUP153 protein, human; D028861:Nuclear Pore Complex Proteins; D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein; D005982:Glutathione Transferase; D003546:Cysteine Endopeptidases; C501833:SENP2 protein, human; C408873:SENP6 protein, human