title: Nucleotide sequence of envelope protein of Japanese encephalitis virus SA14-14-2 adapted to vero cells.
abstract: Live attenuated Japanese encephalitis (JE) virus SA(14)-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA(14)-14-2(Vero). Animal testing showed that SA(14)-14-2(Vero) has an attenuation phenotype similar to that of the parent SA(14)-14-2(PDK) strain in mice.
authors.affiliation: Cheil Jedang Corporation, Research and Development Center, Ichon-Si, Kyeonggi-Do, South Korea.
authors.forename: S P
authors.initials: SP
authors.lastname: Hong
authors.forename: W D
authors.initials: WD
authors.lastname: Yoo
authors.forename: R
authors.initials: R
authors.lastname: Putnak
authors.forename: K H
authors.initials: KH
authors.lastname: Eckels
authors.forename: H M
authors.initials: HM
authors.lastname: Rho
authors.forename: S O
authors.initials: SO
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D004285:Dogs; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins