title: Cloning and expression of class A beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei.
abstract: The beta-lactamase gene blaA(BPS) in Burkholderia pseudomallei was cloned and expressed in Escherichia coli. BPS-1 is a cephalosporinase with an isoelectric point of 7.7. Sequence analysis of BPS-1 revealed conserved motifs typical of class A beta-lactamases and a relationship to the PenA (in B. cepacia) and BlaI (in Yersinia enterocolitica) lineages.
authors.affiliation: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.
authors.forename: Terence K M
authors.initials: TK
authors.lastname: Cheung
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: Patrick C Y
authors.initials: PC
authors.lastname: Woo
authors.forename: K Y
authors.initials: KY
authors.lastname: Yuen
authors.forename: P Y
authors.initials: PY
authors.lastname: Chau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016957:Burkholderia pseudomallei / Q000187:drug effects*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D006723:Hong Kong; D006801:Humans; D007700:Kinetics; D008554:Melioidosis / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013785:Thailand; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases; C462219:blaA protein, Burkholderia pseudomallei