Immobilized gellan sulfate surface for cell adhesion and multiplication: development of cell-hybrid biomaterials using self-produced fibronectin.
A new concept for cell-hybrid biomaterial is proposed in which human unbilical vein endothelial cells (HUVEC) are adhered to an immobilized gellan sulfate (GS) surface. Extra domain A containing fibronectin (EDA(+)FN) released from HUVEC is necessary for cell adhesion and multiplication. The material design in this study is based on these self-released cell adhesion proteins. The interaction between GS and EDA(+)FN was evaluated using the affinity constant (KA); the value obtained was 1.03x10(8) (M(-1)). These results suggest that the adhesion of HUVEC to GS may be supported by the adhesion of EDA(+)FN to GS. We also found that this new material adheres to HUVEC, allowing the reintroduction of EDA(+)FN, which is self-produced by the cell. This material is relatively easy to produce, not requiring the usual coating of adhesion proteins in pretreatment.
Miyamoto
Keiichi
K
Department of Chemistry for Materials, Faculty of Engineering, Mie University, Tsu, Japan. miyamoto@chem.mie-u.ac.jp
Kanemoto
Akiko
A
Hashimoto
Kenichi
K
Tokita
Masayuki
M
Komai
Takashi
T
D002240:Carbohydrate Sequence; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry / Q000378:metabolism*
D005353:Fibronectins; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013431:Sulfates; C048288:gellan gum