title: Mapping neutralizing and non-neutralizing epitopes on the capsid protein of feline calicivirus.
abstract: Neutralizing epitopes on feline calicivirus (FCV) capsid protein were mapped using chimeric capsid proteins recombinant between two FCV isolates that do not show any cross-neutralization. The three chimeric proteins examined were expressed in murine L929 cells employing an MVA/T7 vaccinia virus expression system and inoculated into major histocompatibility complex haplotype-matched C3/HN mice. Based on the neutralizing antibody titre the neutralizing epitope(s) could be mapped to the 5' hypervariable region of the E region or potentially to the C region. The epitopes of some non-neutralizing antibodies were mapped with the same chimeric proteins to the regions B or D and F of the FCV capsid protein.
authors.affiliation: Institute for Medical Microbiology, Infectious and Epidemic diseases, Ludwig Maximilians University Munich, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Geissler
authors.forename: K
authors.initials: K
authors.lastname: Schneider
authors.forename: U
authors.initials: U
authors.lastname: Truyen
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D017927:Calicivirus, Feline / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D017931:DNA Primers; D018604:Epitope Mapping; D051379:Mice; D009500:Neutralization Tests / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers