title: Identification of the alternative splice products encoded by the human protein phosphatase inhibitor-1 gene.
abstract: We have isolated three major cDNA fragments of protein phosphatase inhibitor-1 from human brain and liver by RT-PCR. The 536 bp fragment encoded the wild-type of inhibitor-1 while two other fragments were alternative splice products of the inhibitor-1 gene, which was confirmed by partial genomic DNA sequencing. The 380 bp fragment encoded an in-frame 51-residue-deleted inhibitor-1, named inhibitor-1alpha, and the deletion occurred from residue 84 to 134 of inhibitor-1. The 316 bp fragment termed inhibitor-1beta was derived from an internal deletion of 536 bp fragment. This deletion resulted in an out of frame shift, allowing the 316 bp fragment that encoded the partial sequence of inhibitor-1. Based on the reported mRNA sequence of inhibitor-1 and evidence from our RT-PCR, we suggested that inhibitor-1beta consisted of 132 amino acids of which the N-terminal 61 amino acid sequences were identical to inhibitor-1 while the sequence after residue-61 was markedly different.
authors.affiliation: Department of Research, Buddhist Tzu Chi General Hospital, Hualien 970, Taiwan, Republic of China.
authors.forename: Hsin-tzu
authors.initials: HT
authors.lastname: Liu
authors.forename: Ta-hsien
authors.initials: TH
authors.lastname: Lin
authors.forename: Hann-chorng
authors.initials: HC
authors.lastname: Kuo
authors.forename: Yi-chen
authors.initials: YC
authors.lastname: Chen
authors.forename: Huey-Jen
authors.initials: HJ
authors.lastname: Tsay
authors.forename: Hao-Hsuan
authors.initials: HH
authors.lastname: Jeng
authors.forename: Pai-Chi
authors.initials: PC
authors.lastname: Tsai
authors.forename: Fu-kai
authors.initials: FK
authors.lastname: Shie
authors.forename: Ji-hsiung
authors.initials: JH
authors.lastname: Chen
authors.forename: Hsien-bin
authors.initials: HB
authors.lastname: Huang
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D005091:Exons / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D011506:Proteins; C450539:PPP1R2C protein, human; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1