title: Mapping the fibrinogen-binding domain of serum opacity factor of group a streptococci.
abstract: Serum opacity factor (SOF) is a large, extracellular, and cell-bound protein of group A streptococci that has two known functions, opacification of serum and binding of fibronectin. Herein, we describe a new function of SOF, the binding of fibrinogen. Utilizing purified, truncated recombinant SOF proteins, the fibrinogen-binding domain was localized to a region in the C-terminus of SOF encompassing amino acid residues 844-1047. Western-blot analysis revealed that SOF bound primarily to the beta subunit of fibrinogen. A SOF-negative mutant bound 50% less fibrinogen than did its wild-type parent. Furthermore, fibrinogen blocked the binding of SOF to fibronectin. These data suggest that fibrinogen and fibronectin bind to the same domain within SOF. It remains to be determined whether the binding of fibrinogen to SOF contributes to the virulence of group A streptococci.
authors.affiliation: Department of Medicine, University of Tennessee, Memphis, TN, USA. hcourtney@utmem.edu
authors.forename: Harry S
authors.initials: HS
authors.lastname: Courtney
authors.forename: James B
authors.initials: JB
authors.lastname: Dale
authors.forename: David L
authors.initials: DL
authors.lastname: Hasty
mesh_terms: D001665:Binding Sites; D001667:Binding, Competitive; D015153:Blotting, Western; D005340:Fibrinogen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D013297:Streptococcus pyogenes / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D005353:Fibronectins; D011994:Recombinant Proteins; C021908:opacity factor; D005340:Fibrinogen; D010447:Peptide Hydrolases