title: Structure and evolution of the mitochondrial DNA complete control region in the Drosophila subobscura subgroup.
abstract: The complete A + T-rich region of mitochondrial DNA (mtDNA) has been cloned and sequenced in the species of the Drosophila subobscura subgroup D. subobscura, D. madeirensis and D. guanche. Comparative analysis of these sequences with others already published has identified new sequence motifs that are conserved in Drosophila and other insects. A putative bi-directional promoter and a stop signal are proposed to be involved in the primary mtDNA strand replication of Drosophila. This region strongly resolves relationships of the species included in a phylogenetic analysis, both for closely related species and also at deeper phylogenetic levels when only the left and central domains are taken into account.
authors.affiliation: Centro de Ciências Biologicas e Geologicas, University of Madeira, Penteada, Portugal. brehm@uma.pt
authors.forename: A
authors.initials: A
authors.lastname: Brehm
authors.forename: D J
authors.initials: DJ
authors.lastname: Harris
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: V M
authors.initials: VM
authors.lastname: Cabrera
authors.forename: J M
authors.initials: JM
authors.lastname: Larruga
authors.forename: F M
authors.initials: FM
authors.lastname: Pinto
authors.forename: A M
authors.initials: AM
authors.lastname: González
mesh_terms: D020856:AT Rich Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D004330:Drosophila / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D017344:Genes, Insect; D019901:Locus Control Region; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary; D004272:DNA, Mitochondrial