Functional annotation of a full-length Arabidopsis cDNA collection.
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
Seki
Motoaki
M
Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
Narusaka
Mari
M
Kamiya
Asako
A
Ishida
Junko
J
Satou
Masakazu
M
Sakurai
Tetsuya
T
Nakajima
Maiko
M
Enju
Akiko
A
Akiyama
Kenji
K
Oono
Youko
Y
Muramatsu
Masami
M
Hayashizaki
Yoshihide
Y
Kawai
Jun
J
Carninci
Piero
P
Itoh
Masayoshi
M
Ishii
Yoshiyuki
Y
Arakawa
Takahiro
T
Shibata
Kazuhiro
K
Shinagawa
Akira
A
Shinozaki
Kazuo
K
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D018745:Genome, Plant; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger