title: Studies on serological cross-reaction of Neospora caninum with Toxoplasma gondii and Hammondia heydorni.
abstract: The enzyme-linked immunosorbent assay (ELISA) was used to examine cross-reactivity of Neospora caninum with Toxoplasma gondii and Hammondia heydorni. Anti-T. gondii mouse and cat sera cross-reacted with N. caninum soluble antigen (NLA), but not with the recombinant surface antigen (NcSRS2). Anti-H. heydorni dog sera showed no cross-reactivity with either the NLA antigen or the NcSRS2. Lack of cross-reactivity between anti-H. heydorni sera and N. caninum antigens, and the cross-reactivity of anti-T. gondii sera with the NLA suggest that N. caninum has common antigens to T. gondii except for NcSRS2 based on serology. In light of several studies suggesting a closer relationship between N. caninum and H. heydorni than with T gondii, examination of serological cross-reactivity with N. caninum may be necessary to further classify the parasites in addition to molecular and morphological studies and clarification of the life cycle.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inadacho, Hokkaido, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Florencia G
authors.initials: FG
authors.lastname: Claveria
authors.forename: Kozo
authors.initials: K
authors.lastname: Fujisaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D003429:Cross Reactions; D021741:Eimeriida / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018688:Neospora / Q000145:classification / Q000276:immunology*; D010802:Phylogeny; D014122:Toxoplasma / Q000145:classification / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan