Recombinant baculoviruses as mammalian cell gene-delivery vectors.
The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. Recently, recombinant baculovirus vectors engineered to contain mammalian cell-active promoter elements, have been used successfully for transient and stable gene delivery in a broad spectrum of primary and established mammalian cells. The application of modified baculoviruses for in vivo gene delivery has also been demonstrated. In contrast to other commonly used viral vectors, baculoviruses have the unique property of replicating in insect cells while being incapable of initiating a replication cycle and producing infectious virus in mammalian cells. The viruses can be readily manipulated, accommodate large insertions of foreign DNA, initiate little to no microscopically observable cytopathic effect in mammalian cells and have a good biosafety profile. These attributes will undoubtedly lead to the increased application and continued development of this system for efficient gene delivery into mammalian cells. Who said you can't teach an old dog new tricks?
Kost
Thomas A
TA
Gene Expression and Protein Biochemistry, GlaxoSmithKline Discovery Research, Research Triangle Park, North Carolina 27709, USA. tak4306@gsk.com
Condreay
J Patrick
JP
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D018014:Gene Transfer Techniques; D005814:Genes, Viral; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008322:Mammals / Q000235:genetics* / Q000821:virology*; D015678:Viral Structural Proteins / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D015678:Viral Structural Proteins