Quantitative production of 2-acetamido-2-deoxy-D-glucose from crystalline chitin by bacterial chitinase.
Finely powdered alpha- and beta-chitin can be completely hydrolyzed with chitinase (EC 3.2.1.14) and beta-N-acetylhexosaminidase (EC 3.2.1.52) for the production of 2-acetamido-2-deoxy-D-glucose (GlcNAc). Crude chitinase from Burkholderia cepacia TU09 and Bacillus licheniformis SK-1 were used to digest alpha- and beta-chitin powder. Chitinase from B. cepacia TU09 produced GlcNAc in greater than 85% yield from beta- and alpha-chitin within 1 and 7 days, respectively. B. licheniformis SK-1 chitinase completely hydrolyzed beta-chitin within 6 days, giving a final GlcNAc yield of 75%, along with 20% of chitobiose. However, only a 41% yield of GlcNAc was achieved from digesting alpha-chitin with B. licheniformis SK-1 chitinase.
Pichyangkura
Rath
R
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. rath.p@chula.ac.th
Kudan
Sanya
S
Kuttiyawong
Kamontip
K
Sukwattanasinitt
Mongkol
M
Aiba
Sei-ichi
S
D000117:Acetylglucosamine / Q000096:biosynthesis*; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism; D016956:Burkholderia cepacia / Q000201:enzymology; D002686:Chitin / Q000737:chemistry / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism*; D003460:Crystallization; D006868:Hydrolysis; D013997:Time Factors; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases; D001619:beta-N-Acetylhexosaminidases; D000117:Acetylglucosamine