Molecular motors: single-molecule recordings made easy.
A fusion protein of kinesin and gelsolin binds a short actin filament which can be visualized using a standard fluorescence microscope. This technique has provided new insight into the mechanism of kinesin action, and in principle it can be extended to allow single-molecule assays of any protein.
Diez
Stefan
S
Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.
Schief
William R
WR
Howard
Jonathon
J
D018260:Gelsolin / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D010590:Phalloidine / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry
D018260:Gelsolin; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D049452:Green Fluorescent Proteins; D010590:Phalloidine; D016547:Kinesins