Physicochemical and functional properties of buckwheat protein product.
This study was conducted to compare the physicochemical and functional properties of buckwheat protein product (BWP), soy protein isolate (SPI), and casein. BWP was prepared from buckwheat flour by the method including alkaline extraction and isoelectric precipitation. The amino acid composition of BWP was very similar to that of buckwheat flour. The protein solubility (PS) of BWP was much greater than that of SPI at all pH levels (pH 2-10) but lower than that of casein at pH 7-10. The isoelectric point of BWP was around pH 4. The higher aromatic hydrophobicities (ARH) of BWP, SPI, and casein were obtained at lower pH levels (pH 2-3). The emulsifying stability (ES) of BWP was lower than those of SPI and casein at high pH levels (pH 7-10). At all pH levels, BWP formed a thin emulsion. Regression analysis showed that the ARH of BWP was significantly associated with the ES. Although the water holding capacity of BWP was quite lower than that of SPI, its fat absorption capacity was slightly higher than those of SPI and casein. These results indicated that the physicochemical properties of BWP were different from those of SPI or casein. Thus, BWP is a potential source of functional protein for possible food application.
Tomotake
Hiroyuki
H
Iida Women's Junior College, Iida, 395-0812 Japan; Development, Health Care, Kissei Pharmaceutical Company, Ltd., Matsumoto, 399-8710 Japan. tomotake@iidawjc.ac.jp
Shimaoka
Iwao
I
Kayashita
Jun
J
Nakajoh
Misao
M
Kato
Norihisa
N
D000596:Amino Acids / Q000032:analysis; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003314:Corn Oil / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D019612:Fagopyrum / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D012044:Regression Analysis; D012995:Solubility; D030262:Soybean Proteins / Q000737:chemistry / Q000378:metabolism; D014783:Viscosity; D014867:Water / Q000737:chemistry
D000596:Amino Acids; D002364:Caseins; D004655:Emulsions; D010940:Plant Proteins; D030262:Soybean Proteins; D014867:Water; D003314:Corn Oil