title: Shear stress induces caveolin-1 translocation in cultured endothelial cells.
abstract: Considering that vascular endothelial caveolae could be flow sensors converting mechanical stimuli into chemical signals transmitted into the cell, this work studied, in vitro, the change of caveolin-1 expression and distribution of cultured endothelial cells exposed to laminar flows. Experimental results showed that, in control cells, caveolin-1 were primarily localized on the cell surface, and presented some local concentrations. In cells exposed to laminar flows, caveolin-1 distribution showed a time-dependent variation. After 24 h of shear (1.0 Pa), the expression of caveolin-1 increased and a local caveolin-1 concentration was found, in most cells, at the upstream side of the cell body where the hydrostatic pressure and the spatial gradient of shear stress were at a maximum. As a comparison, tumor necrosis factor-a induced a decrease of caveolin-1 in the cells.
authors.affiliation: Group Mechanics and Cell and Tissue Engineering, LEMTA-UMR-7563 CNRS/INPL/UHP, Vandoeuvre-lès-Nancy, France.
authors.forename: R J
authors.initials: RJ
authors.lastname: Sun
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D051242:Caveolin 1; D022461:Caveolins / Q000096:biosynthesis / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D015398:Signal Transduction; D013314:Stress, Mechanical; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D014409:Tumor Necrosis Factor-alpha