title: Endogenous adipocyte precursor cells for regenerative soft-tissue engineering.
abstract: Subcutaneous injection of reconstituted basement membrane (Matrigel) in combination with basic fibroblast growth factor induces de novo adipogenesis in which endogenous precursor cells invade the artificially formed Matrigel space, proliferate and differentiate to form adipose tissue. Since this adipogenesis offers us a novel approach for soft-tissue reconstruction without transplanting preadipocytes, the early process was examined by optical and electron microscopy. Formation of multiple layers of fibroblast-like cells at the surface of Matrigel implant was the first response of connective tissue. The cells within four to five layers proximal to Matrigel implant acquired a thick cytoplasm and an enlarged nucleus, and they invaded Matrigel space together with endothelial cells which caused neovascularization. Phagocytotic incorporation and digestion of Matrigel components by well-developed lysosomes appeared to be a stimulus of fibroblast-like cells to mature depending on proximity to Matrigel. The fibroblast-like cells often contacted to the outer surface of capillary over a large area and rapidly accumulated lipid droplets. Electron microscopy of the developing adipocytes showed a well-organized smooth endoplasmic reticulum and mitochondria. This investigation thus revealed the characteristics of adipocyte precursor cells, which can be recruited for regenerative engineering of soft tissues.
authors.affiliation: Department of Plastic and Reconstructive Surgery, School of Medicine, Nagoya University, Showa, Nagoya, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Toriyama
authors.forename: Nobuko
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: Junzou
authors.initials: J
authors.lastname: Kitoh
authors.forename: Rie
authors.initials: R
authors.lastname: Tajima
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Inou
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Shuhei
authors.initials: S
authors.lastname: Torii
mesh_terms: D017667:Adipocytes / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D003238:Connective Tissue / Q000502:physiology* / Q000648:ultrastructure; D004338:Drug Combinations; D007797:Laminin / Q000502:physiology; D051379:Mice; D008854:Microscopy, Electron; D011509:Proteoglycans / Q000502:physiology; D012038:Regeneration; D023822:Tissue Engineering
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen