title: miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.
abstract: Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6. Reduction in SMN protein results in Spinal muscular atrophy (SMA), a common neurodegenerative disease. The SMN complex has critical functions in the assembly/restructuring of diverse ribonucleoprotein (RNP) complexes. Here we report that Gemin3 and Gemin4 are also in a separate complex that contains eIF2C2, a member of the Argonaute protein family. This novel complex is a large approximately 15S RNP that contains numerous microRNAs (miRNAs). We describe 40 miRNAs, a few of which are identical to recently described human miRNAs, a class of small endogenous RNAs. The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry & Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148, USA.
authors.forename: Zissimos
authors.initials: Z
authors.lastname: Mourelatos
authors.forename: Josée
authors.initials: J
authors.lastname: Dostie
authors.forename: Sergey
authors.initials: S
authors.lastname: Paushkin
authors.forename: Anup
authors.initials: A
authors.lastname: Sharma
authors.forename: Bernard
authors.initials: B
authors.lastname: Charroux
authors.forename: Linda
authors.initials: L
authors.lastname: Abel
authors.forename: Juri
authors.initials: J
authors.lastname: Rappsilber
authors.forename: Matthias
authors.initials: M
authors.lastname: Mann
authors.forename: Gideon
authors.initials: G
authors.lastname: Dreyfuss
mesh_terms: D000818:Animals; D060565:Argonaute Proteins; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D003001:Cloning, Molecular; D055541:DEAD Box Protein 20; D053487:DEAD-box RNA Helicases; D039642:Eukaryotic Initiation Factors; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D035683:MicroRNAs; D015778:Minor Histocompatibility Antigens; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010448:Peptide Initiation Factors / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020365:RNA Helicases / Q000378:metabolism; D016372:RNA, Antisense / Q000737:chemistry* / Q000145:classification; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry* / Q000145:classification; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear; D055532:SMN Complex Proteins
chemical_list: C115848:AGO1 protein, human; D060565:Argonaute Proteins; D039642:Eukaryotic Initiation Factors; C108381:GEMIN2 protein, human; C406815:GEMIN4 protein, human; C449808:GEMIN5 protein, human; C450836:GEMIN6 protein, human; C486731:Gemin5 protein, mouse; C486734:Gemin6 protein, mouse; D035683:MicroRNAs; D015778:Minor Histocompatibility Antigens; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010448:Peptide Initiation Factors; D016372:RNA, Antisense; D012333:RNA, Messenger; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055532:SMN Complex Proteins; C491561:Sip1 protein, mouse; C120168:DDX20 protein, human; D055541:DEAD Box Protein 20; C485624:Ddx20 protein, mouse; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases