title: Expression of JC virus T-antigen in a patient with MS and glioblastoma multiforme.
abstract: OBJECTIVE
  To investigate the presence of human polyomavirus JC virus genome and the expression of the viral oncoprotein T-antigen in neoplastic cells of a patient with MS and a glioblastoma multiforme.
  
  
  BACKGROUND
  The postmortem examination of an immunocompetent patient with a neurologic disorder revealed the concurrence of MS plaques in the white matter of the brain and a glioblastoma multiforme in the region of the thalamus.
  
  
  RESULTS
  PCR analysis of DNA from demyelinated plaques and the tumor area using primers derived from specific regions of the JC virus genome revealed the presence of viral DNA corresponding to the viral early and late genes. Further examination of the samples for the JC virus regulatory region identified the presence of sequences identical to JC virus Mad-4 and JC virus W1 viral isolates in the tumor and the demyelinated regions. Results from immunohistochemistry showed the detection of the viral early protein, T-antigen, and the cellular tumor suppressor protein, p53, in the nuclei of neoplastic cells. Interestingly, expression of T-antigen, but not p53, was observed in neurofilament-positive cells with neuronal morphology and in glial fibrillary acidic protein-positive astrocytes in the cortex juxtaposed to the MS plaques. Examination of viral late gene expression by immunohistochemistry showed no evidence for viral capsid proteins, thus ruling out productive replication of JC virus in the tumor and MS demyelinated plaques.
  
  
  CONCLUSIONS
  These observations provide molecular and clinical evidence of the association of JC virus in the brain of a patient with concurrent glioblastoma multiforme and MS.
authors.affiliation: Center for Neurovirology and Cancer Biology, College of Science and Technology, Temple University, Philadelphia, PA 19122, USA.
authors.forename: L
authors.initials: L
authors.lastname: Del Valle
authors.forename: S
authors.initials: S
authors.lastname: Delbue
authors.forename: J
authors.initials: J
authors.lastname: Gordon
authors.forename: S
authors.initials: S
authors.lastname: Enam
authors.forename: S
authors.initials: S
authors.lastname: Croul
authors.forename: P
authors.initials: P
authors.lastname: Ferrante
authors.forename: K
authors.initials: K
authors.lastname: Khalili
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis* / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology / Q000821:virology*; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000235:genetics / Q000473:pathology / Q000821:virology*; D013788:Thalamus / Q000378:metabolism / Q000473:pathology / Q000821:virology*
chemical_list: D000952:Antigens, Polyomavirus Transforming