Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
Expression of the two Bacillus subtilis genes encoding L-asparaginase is controlled by independent regulatory factors. The ansZ gene (formerly yccC) was shown by mutational analysis to encode a functional L-asparaginase, the expression of which is activated during nitrogen-limited growth by the TnrA transcription factor. Gel mobility shift and DNase I footprinting experiments indicate that TnrA regulates ansZ expression by binding to a DNA site located upstream of the ansZ promoter. The expression of the ansA gene, which encodes the second L-asparaginase, was found to be induced by asparagine. The ansA repressor, AnsR, was shown to negatively regulate its own expression.
Fisher
Susan H
SH
Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118, USA. shfisher@bu.edu
Wray
Lewis V
LV
D001215:Asparaginase / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006836:Hydro-Lyases; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D001215:Asparaginase; D006836:Hydro-Lyases; C115994:rluC protein, E coli; D009584:Nitrogen