Detection of minimal residual disease in peripheral blood stem cells from two acute myeloid leukemia patients with trisomy 8 predicts early relapse after autologous bone marrow transplantation.
We report two cases of acute myeloid leukemia (AML) French-American-British M4 classification with trisomy 8 at diagnosis as the sole chromosome abnormality. Both patients were treated with the GIMEMA AML-10 protocol and underwent autologous bone marrow transplantation (ABMT) in hematologic remission. Peripheral blood stem cells (PBSC), and bone marrow in one patient, were collected after consolidation therapy and tested by fluorescence in situ hybridization (FISH) analysis with an alpha-satellite probe for chromosome 8. It revealed that all samples were positive for minimal residual disease (MRD) as the value of trisomic cells exceeded the mean +3 standard deviations of the controls. ABMT was done following a myeloablative regimen (busulphan/cyclophosphamide) and PBSC were reinfused. Both patients relapsed, 4 and 2 months, respectively, after autotransplant. Although more data are needed, these results suggest that the persistence of MRD, as detected by FISH, in stem cell collections, is associated with a poor outcome in AML patients with trisomy 8 undergoing ABMT.
Scaravaglio
Patrizia
P
Dipartimento di Scienze Cliniche e Biologiche, Azienda Ospedaliera San Luigi Gonzaga, Orbassano, Torino, Italy.
Guglielmelli
Tommasina
T
Giugliano
Emilia
E
Marmont
Filippo
F
Audisio
Ernesta
E
Gallo
Eugenio
E
Saglio
Giuseppe
G
Rege-Cambrin
Giovanna
G
D000208:Acute Disease; D000328:Adult; D016026:Bone Marrow Transplantation; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology / Q000628:therapy; D008297:Male; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000235:genetics; D012008:Recurrence; D014182:Transplantation, Autologous; D014314:Trisomy