Hepatic vagotomy disrupts somatotropin-induced protein selection.
Rats treated with somatotropin (STH) and allowed to self-select between diets varying in protein content will consume more of the high-protein diet. The objective of this study was to determine the role of the hepatic vagus nerve in this ability to select protein. Female Sprague-Dawley rats (n=40) received a hepatic vagotomy (HVAGX) or a sham surgery. Postsurgery, the rats were maintained on pelleted diets for 2 weeks, after which the rats were adapted to selecting between powdered diets with 5% casein and 30% casein. After a 7-day adaptation to diet selection, rats in each surgical treatment group were treated with STH (4 mg/day) or physiological saline for 14 days. Body weight and intake were recorded daily. STH treatment increased growth rate to a similar degree in both sham and HVAGX groups. Despite causing an increase in total food intake, there was no effect of HVAGX alone on body weight. Relative to the sham-saline group, sham-STH in treated rats had greater total food intake that was accounted for entirely by increased consumption of the 30% protein diet and no change in intake of the 5% diet. In contrast, HVAGX+STH rats exhibited 20-30% increases in consumption of both the 5% and 30% protein diets. Thus, the HVAGX+STH rats recognized an increased need for protein, but were unable to distinguish between the high- and low-protein diets and selected more of both. The data suggest that the ability to alter diet selection in response to a stimulation of protein accretion is at least partially mediated through the liver and hepatic branch of the vagus nerve.
Phositlimpagul
A
A
Animal and Dairy Science Department, University of Georgia, Athens, GA 30602, USA.
Edwards
G L
GL
Azain
M J
MJ
D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology; D000596:Amino Acids / Q000097:blood; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004032:Diet; D004044:Dietary Proteins / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects / Q000502:physiology; D005260:Female; D005518:Food Preferences / Q000187:drug effects* / Q000502:physiology*; D013006:Growth Hormone / Q000494:pharmacology*; D008099:Liver / Q000294:innervation*; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014628:Vagotomy
D000596:Amino Acids; D004044:Dietary Proteins; D013006:Growth Hormone