title: 1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins.
abstract: The implementation of [13Calpha,13C',15N,2Halpha] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of 1JCalpha,Cbeta couplings enables us to measure 1JCalpha,C' scalar and 1DCalpha,C' residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for 13C constant time experiments.
authors.affiliation: School of Biochemistry and Molecular Biology, University of Leeds, UK.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Giesen
authors.forename: Lynnette C
authors.initials: LC
authors.lastname: Bae
authors.forename: Christa L
authors.initials: CL
authors.lastname: Barrett
authors.forename: Jason A
authors.initials: JA
authors.lastname: Chyba
authors.forename: Michael M
authors.initials: MM
authors.lastname: Chaykovsky
authors.forename: Minn-Chang
authors.initials: MC
authors.lastname: Cheng
authors.forename: Jenny H
authors.initials: JH
authors.lastname: Murray
authors.forename: Ed J
authors.initials: EJ
authors.lastname: Oliver
authors.forename: Sarah M
authors.initials: SM
authors.lastname: Sullivan
authors.forename: Jonathan Miles
authors.initials: JM
authors.lastname: Brown
authors.forename: Steve W
authors.initials: SW
authors.lastname: Homans
mesh_terms: D000577:Amides; D016880:Anisotropy; D002247:Carbon Isotopes; D003903:Deuterium; D006207:Half-Life; D008280:Magnetics; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011522:Protons; D012680:Sensitivity and Specificity; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D000577:Amides; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D011506:Proteins; D011522:Protons; D025801:Ubiquitin; D003903:Deuterium