Amino acid concentrations in the blood of the jugular vein and peripheral artery after traumatic brain injury: decreased release of glutamate into the jugular vein in the early phase.
The gross behavior of excitatory amino acids in patients with traumatic brain injury (TBI), including uptake, transport, metabolism, and clearance, was investigated by analysis of the levels of 41 amino acids in the blood of the jugular vein (JV), which is the primary venous drainage conduit of the brain, and a peripheral artery. Blood samples from the JV and a peripheral artery of eight patients with TBI were collected at 6 h, 6 to 24 h, and over 24 h after TBI, and analyzed using high performance liquid chromatography. Blood samples from 101 normal subjects were also measured. The levels of glutamate (Glu), gamma-aminobutyric acid (GABA), aspartate, glutamine, and cystine deviated from the normal range, and were considered pathological. The level of Glu in the JV was significantly lower than that in the artery (p < 0.05), and the level of GABA in the JV was significantly higher than that in the artery (p < 0.01), but the other three amino acids showed no significant differences. Significantly chronological changes in the difference between the blood levels in the JV and artery were observed for Glu. Measurement of the Glu level in the JV and artery may indicate gross metabolic change in the brain following TBI.
Suzuki
Michiyasu
M
Department of Neurosurgery, Yamaguchi University School of Medicine, Ube, Japan. michi@po.cc.yamaguchi-u.ac.jp
Kudo
Akira
A
Sugawara
Atushi
A
Yoshida
Kenji
K
Kubo
Yoshitaka
Y
Suzuki
Takeshi
T
Ogasawara
Kuniaki
K
Doi
Mamoru
M
Ogawa
Akira
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000596:Amino Acids / Q000097:blood*; D001158:Arteries; D001224:Aspartic Acid / Q000097:blood; D001930:Brain Injuries / Q000097:blood*; D003553:Cystine / Q000097:blood; D005260:Female; D018698:Glutamic Acid / Q000097:blood*; D005973:Glutamine / Q000097:blood; D006801:Humans; D007601:Jugular Veins; D008297:Male; D008875:Middle Aged; D005680:gamma-Aminobutyric Acid / Q000097:blood
D000596:Amino Acids; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D003553:Cystine; D005680:gamma-Aminobutyric Acid