title: How does tmRNA move through the ribosome?
abstract: To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon-anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.
authors.affiliation: Department of Chemistry, M.V. Lomonosov Moscow State University, 119899, Moscow, Russia.
authors.forename: Pavel V
authors.initials: PV
authors.lastname: Ivanov
authors.forename: Maria I
authors.initials: MI
authors.lastname: Zvereva
authors.forename: Olga V
authors.initials: OV
authors.lastname: Shpanchenko
authors.forename: Olga A
authors.initials: OA
authors.lastname: Dontsova
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Bogdanov
authors.forename: Galina V
authors.initials: GV
authors.lastname: Aglyamova
authors.forename: Valery I
authors.initials: VI
authors.lastname: Lim
authors.forename: Yoshika
authors.initials: Y
authors.lastname: Teraoka
authors.forename: Knud H
authors.initials: KH
authors.lastname: Nierhaus
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; C096222:tmRNA