title: [The influence of smoking on the estradiol level in the preovulatory follicular fluid].
abstract: The aim of the present study was to test the relationship between the objectively measured intrafollicular exposure to the tobacco smoke compounds and the estradiol level in the preovulatory follicular fluid. In 30 IVF-ET patients (14 smokers and 16 non-smokers) levels of cotinine (C-FF) and 17 beta-estradiol (E2-FF) were quantified in the follicular fluid by means of radioimmunoassay. In smokers (C-FF from 62.21 to 595.00 ng/ml) the mean E2-FF was 85.07 nmol/l, while in the non-smokers (C-FF from 1.20 to 15.62 ng/ml) the respective value amounted to 109.19 nmol/l. The difference was statistically significant (p = 0.005). The obtained results support the growing body of evidence that tobacco smoking negatively influences endocrine function of the Graafian follicle.
authors.affiliation: III Katedry i Kliniki Ginekologii A.M. w Lublinie.
authors.forename: T
authors.initials: T
authors.lastname: Paszkowski
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D003367:Cotinine / Q000378:metabolism; D004624:Embryo Transfer; D004958:Estradiol / Q000097:blood / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D009538:Nicotine / Q000378:metabolism; D011247:Pregnancy; D011863:Radioimmunoassay; D012907:Smoking / Q000009:adverse effects*; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D004958:Estradiol; D009538:Nicotine; D003367:Cotinine