Basic fibroblast growth factor induces TGF-beta release in an isoform and glioma-specific manner.
The aim of the current study was to determine whether basic fibroblast growth factor (bFGF), regulates the release of transforming growth factor-beta1 (TGF-beta1) from C6 glioma cells. The results of the study show that bFGF (2, 5 and 10 ng/ml) dose dependently induced the release of TGF-beta1 from C6 glioma cells, with the 10 ng/ml dose inducing a 2- to 3-fold increase of TGF-beta1 levels. This effect was evident as early as 6 h following treatment, with maximal levels observed at 18 h. The effect of bFGF was largely on latent TGF-beta1, and was isoform specific, as bFGF had no effect on TGF-beta2 release. The bFGF effect on TGF-beta1 was also glioma specific, as no such stimulatory effect was observed in rat cortical astrocytes.
Dhandapani
Krishnan M
KM
Institute of Molecular Medicine and Genetics, Neurobiology Program, and Department of Neurology, 1120 15th Street, Medical College of Georgia, Augusta, GA 30912, USA.
Wade
Marlene F
MF
Mahesh
Virendra B
VB
Brann
Darrell W
DW
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D020033:Protein Isoforms; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2