Prevalence of herpesvirus DNA in MS patients and healthy blood donors.
OBJECTIVE
The aim of this study is to determine the DNA prevalence of different members of Herpesviridae in multiple sclerosis (MS) patients and to describe the possible effect of beta-interferon treatment on such prevalence.


METHODS
With a nested polymerase chain reaction (PCR) assay we have studied the DNA of the peripheral blood mononuclear cells (PBMCs) of 204 whole blood samples, [102 from patients with relapsing-remitting MS (RRMS), of which 62 were treated with beta-interferon, and 102 from healthy blood donors].


RESULTS
We only found a statistically significant difference for human herpesvirus type 6 (HHV-6) DNA prevalence (P < 0.0001): HHV-6 is 2.26 times more frequent in MS patients than in healthy donors. There was no difference in the HHV-6 prevalence between beta-interferon treated and untreated patients.


CONCLUSIONS
1. Among the herpesviruses, HHV-6 was the only one showing altered prevalence. This either indicates that HHV-6 is involved in the pathogenesis of MS, or it simply indicates that MS influences latency or reactivation of HHV-6 without any direct involvement of HHV-6 in the disease process of MS. 2. Treatment with beta-interferon does not make a difference on the DNA prevalence of the herpesviruses studied in our MS patients.
Alvarez-Lafuente
R
R
Clinical Microbiology Service, Hospital Clínico San Carlos, Madrid, Spain.
Martín-Estefanía
C
C
de las Heras
V
V
Castrillo
C
C
Cour
I
I
Picazo
J J
JJ
Varela De Seijas
E
E
Arroyo
R
R
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000328:Adult; D001782:Blood Donors; D004279:DNA, Viral / Q000097:blood* / Q000187:drug effects* / Q000235:genetics; D005260:Female; D006564:Herpesviridae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016899:Interferon-beta / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000188:drug therapy* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012016:Reference Values
D000276:Adjuvants, Immunologic; D004279:DNA, Viral; D016899:Interferon-beta