title: Porcine sperm factor supports activation and development of bovine nuclear transfer embryos.
abstract: A study was undertaken to determine whether injection of porcine sperm factors (pSF), which trigger oscillations in intracellular calcium concentration ([Ca(2+)](i)) in mammalian oocytes, could be used to activate bovine oocytes during nuclear transfer. To date, only combined treatments that induce a monotonic rise in [Ca(2+)](i) and inhibit either phosphorylation or protein synthesis have been utilized in nuclear transfer. Several doses of pSF were tested. Injection of 5 mg/ml pSF triggered [Ca(2+)](i) oscillations that resembled those associated with fertilization with respect to amplitude and periodicity, and as a result, a high percentage of oocytes underwent activation. Furthermore, this concentration of pSF supported in vitro and in vivo development up to 60-90 days of gestation, comparable to development in control nuclear transfer embryos. Nevertheless, neither activation procedure supported development as well as did fertilization. The effectiveness of pSF as an activating agent in bovine oocytes may have been compromised because pSF was unable to support oscillations past 3-5 h postinjection and a second injection was necessary to extend the [Ca(2+)](i) oscillations. Likewise, a single injection of pSF failed to trigger downregulation of the inositol 1,4,5-trisphosphate receptor 1 subtype, whereas a second injection downregulated the receptor in a manner similar to that seen in fertilized oocytes. These results demonstrate that soluble factor(s) from porcine sperm can support early development in bovine nuclear transfer embryos; however, the efficacy may be limited because of the premature cessation of the induced oscillations.
authors.affiliation: Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA.
authors.forename: Jason G
authors.initials: JG
authors.lastname: Knott
authors.forename: Kasinathan
authors.initials: K
authors.lastname: Poothapillai
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.forename: Chang Li
authors.initials: CL
authors.lastname: He
authors.forename: Rafael A
authors.initials: RA
authors.lastname: Fissore
authors.forename: James M
authors.initials: JM
authors.lastname: Robl
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects; D002417:Cattle; D019976:Cloning, Organism; D015536:Down-Regulation / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005865:Gestational Age; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008297:Male; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects; D013094:Spermatozoa / Q000737:chemistry*; D013552:Swine; D014020:Tissue Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D018160:Receptors, Cytoplasmic and Nuclear; D014020:Tissue Extracts; D002118:Calcium