title: Cryopreservation of cultivated and wild Arachis species embryonic axes using desiccation and vitrification methods.
abstract: The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.
authors.affiliation: Laboratório de Micropropagação e Transformação de Plantas, Universidade do Estado do Rio de Janeiro, Brazil.
authors.forename: R F
authors.initials: RF
authors.lastname: Gagliardi
authors.forename: G P
authors.initials: GP
authors.lastname: Pacheco
authors.forename: J F M
authors.initials: JF
authors.lastname: Valls
authors.forename: E
authors.initials: E
authors.lastname: Mansur
mesh_terms: D010367:Arachis / Q000196:embryology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003890:Desiccation / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009584:Nitrogen; D018520:Plant Shoots / Q000254:growth & development; D020127:Recovery of Function; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*; D013395:Sucrose / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D013395:Sucrose; D009584:Nitrogen; D005990:Glycerol; D004121:Dimethyl Sulfoxide