title: DNA repair dependent NAD+ metabolism is impaired in cells from patients with Fanconi's anemia.
abstract: In vitro cultivated fibroblasts derived either from patients with Fanconi's anemia (FA) or from healthy probands were analyzed for their DNA repair-dependent NAD+ metabolism. No difference in NAD+ pools was found. NAD+ consumption after cell damage by u.v. irradiation was, however, significantly reduced in FA cells. Several FA cell lines had a lowered ability to transfer ADP-ribose to acid-precipitable material. Additionally, a decreased activity of NAD: protein ADP-ribosyltransferase was found for three FA cell lines. Our data indicate, that FA is accompanied by a defective NAD+ metabolism during DNA repair.
authors.affiliation: Institut für Biochemie (Nat. Fak.), Universität Innsbruck, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Klocker
authors.forename: B
authors.initials: B
authors.lastname: Auer
authors.forename: M
authors.initials: M
authors.lastname: Hirsch-Kauffmann
authors.forename: H
authors.initials: H
authors.lastname: Altmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Burtscher
authors.forename: M
authors.initials: M
authors.lastname: Schweiger
mesh_terms: D002457:Cell Extracts; D002463:Cell Membrane Permeability; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D009243:NAD / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
chemical_list: D002457:Cell Extracts; D009243:NAD; D014316:Tritium; D011065:Poly(ADP-ribose) Polymerases