title: A Fourier analysis approach for capillary polarimetry.
abstract: A new method of fringe interrogation based on Fourier analysis was implemented and tested for a capillary polarimetry detector. It has significant advantages over the previously employed depth of modulation (DOM) approach, including speed and alignment insensitivity. The new and old methods were compared using a set of interference fringes typically used to facilitate nanoliter volume polarimetric determinations. Polarimetric response was calculated with both methods over the range from 0 degrees to 180 degrees. The results were found to be in good agreement with Malus Law and indicate that an fast Fourier transform (fft) could be used for real-time capillary scale polarimetry in a probe volume of 40 nL.
authors.affiliation: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Markov
authors.forename: Kelly
authors.initials: K
authors.lastname: Swinney
authors.forename: Kristin
authors.initials: K
authors.lastname: Norville
authors.forename: David
authors.initials: D
authors.lastname: Lu
authors.forename: Darryl J
authors.initials: DJ
authors.lastname: Bornhop
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D015203:Reproducibility of Results