title: Mammalian germ-line transgenesis by transposition.
abstract: Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
authors.affiliation: The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Dupuy
authors.forename: Karl
authors.initials: K
authors.lastname: Clark
authors.forename: Corey M
authors.initials: CM
authors.lastname: Carlson
authors.forename: Sabine
authors.initials: S
authors.lastname: Fritz
authors.forename: Ann E
authors.initials: AE
authors.lastname: Davidson
authors.forename: Karra M
authors.initials: KM
authors.lastname: Markley
authors.forename: Ken
authors.initials: K
authors.lastname: Finley
authors.forename: Colin F
authors.initials: CF
authors.lastname: Fletcher
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Ekker
authors.forename: Perry B
authors.initials: PB
authors.lastname: Hackett
authors.forename: Sandra
authors.initials: S
authors.lastname: Horn
authors.forename: David A
authors.initials: DA
authors.lastname: Largaespada
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D019895:Transposases / Q000235:genetics / Q000502:physiology
chemical_list: D004251:DNA Transposable Elements; D019895:Transposases