title: High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR amplified DNA.
abstract: We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp.
authors.affiliation: Finsen Laboratory, Finsen Center, Rigshospitalet, Strandboulevarden 49, DK-2100, Copenhagen, Denmark. jacob.larsen@finsenlab.dk
authors.forename: J
authors.initials: J
authors.lastname: Larsen
authors.forename: A M
authors.initials: AM
authors.lastname: Ottesen
authors.forename: M
authors.initials: M
authors.lastname: Kirchhoff
authors.forename: C
authors.initials: C
authors.lastname: Lundsteen
authors.forename: J K
authors.initials: JK
authors.lastname: Larsen
mesh_terms: D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000032:analysis; D017353:Gene Deletion; D006801:Humans; D015448:Leukemia, B-Cell / Q000175:diagnosis / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm