Human 8-oxoguanine DNA glycosylase 1 mRNA expression as an oxidative stress exposure biomarker of cooking oil fumes.
Epidemiological studies have indicated that the exposure to carcinogenic components formed during the cooking of food might be associated with lung cancer risk of Chinese women. Previous studies have confirmed that cooking oil fumes from frying fish (COF) contained relatively high amount of benzo[a]pyrene, 2-methyl-3,8-dimethylimidazo[4,5-f] qunoxaline, benzene, and 1,3-butadiene, reported in fumes from heated soybean oil. Thus, we consider that oxidative stress induced by COF may play a role in lung cancer development among Chinese women. To verify whether the oxidative DNA damage was induced by COF, high-performance liquid chromatography (HPLC) analysis data showed that the levels of 8-hydroxydeoxyguanine (8-OH dG) were increased in a dose-dependent manner when calf thymus DNA reacted with various concentrations of COF. Since human 8-oxoguanine DNA glycosylase 1 (hOGG1) was a repair enzyme for removing 8- OH dG from damaged DNA, we hypothesized that hOGG1 mRNA may be used to assess the risk of oxidative damage induced by the exposure of COF. The results from reverse-transcription polymerase chain reaction showed that the hOGG1 mRNA expression was induced by hydrogen peroxide (H2O2) and COF in human lung adenocarcinoma CL-3 cells. To elucidate whether hOGG1 mRNA expression was an exposure biomarker of COF, a cross-sectional study of 238 subjects including 94 professional cooks, 43 housewives, and 101 COF-nonexposed control subjects was conducted. The hOGG1 mRNA expression frequencies of COF-exposed cooks (27 of 94, 28.7%) and housewives (6 of 43, 14%) were significantly higher than those of control subjects (4 of 101, 4%). After adjusting for age, sex, and smoking and drinking status, the odds risks (ORs) of housewives versus control and cooks versus control were 3.94 (95% confidence interval [CI] = 0.95-16.62) and 10.12 (95% CI = 2.83-36.15), respectively. These results indicated that hOGG1 may be adequate to act as an exposure biomarker to assess the oxidative DNA damage induced by COF. This also suggests that oxidative stress induced by COF may play a role in lung cancer development among Chinese women.
Cherng
Shur-Hueih
SH
Institute of Toxicology, Chung Shan Medical University, Taichung, Taiwan, Republic of China.
Huang
Kuo Hao
KH
Yang
Sen-Chih
SC
Wu
Tzu-Chin
TC
Yang
Jia-Ling
JL
Lee
Huei
H
D000230:Adenocarcinoma / Q000209:etiology*; D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002273:Carcinogens / Q000009:adverse effects*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D003430:Cross-Sectional Studies; D004249:DNA Damage; D017931:DNA Primers; D043382:DNA-Formamidopyrimidine Glycosylase; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000209:etiology*; D009699:N-Glycosyl Hydrolases / Q000096:biosynthesis*; D016017:Odds Ratio; D018384:Oxidative Stress; D010938:Plant Oils / Q000009:adverse effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D013950:Thymus Gland
D015415:Biomarkers; D002273:Carcinogens; D017931:DNA Primers; D010938:Plant Oils; D012333:RNA, Messenger; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase