title: [Changes of mRNA expression of IL-1beta and TNF-alpha in rat brains after repeated exposures to +Gz].
abstract: Objective. To study changes of mRNA expression of IL-1beta and TNF-alpha in rat brains after repeated exposures to +Gz. Method. Twenty conscious SD rats served as the subjects. They were randomly divided into 5 groups. Using an animal centrifuge, control rats (n = 4) were exposed to +1 Gz and experimental rats (n = 16) were exposed to +14 Gz three times, each for 45 s with 30 min interval in between. The rat brains were taken 30 min, 6 h, 24 h and 48 h after the last centrifuge run and total RNA was isolated. mRNA expression levels of IL-1beta and TNF-alpha in rat brain were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Result. mRNA expression levels of IL-1beta and TNF-alpha in rat brains taken 30 min, 6 h and 24 h after repeated +Gz exposures were significantly higher than those in control rats, but returned to normal after 48 h. Conclusion. It suggested that mRNA expressions of IL-1beta and TNF-alpha in rat brains can be stimulated by repeated +Gz exposures and the increased expression of IL-1beta and TNF-alpha may play a role in the pathologic course of brain damage induced by +Gz exposures, but the damage is reversible.
authors.affiliation: Clinical Aeromedical Center, General Hospital of the Air Force, Beijing, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Liu
authors.forename: Q
authors.initials: Q
authors.lastname: Cai
authors.forename: G Y
authors.initials: GY
authors.lastname: Ji
authors.forename: Z
authors.initials: Z
authors.lastname: Zhan
authors.forename: J D
authors.initials: JD
authors.lastname: Jiang
authors.forename: M C
authors.initials: MC
authors.lastname: Zhu
mesh_terms: D000054:Acceleration; D000818:Animals; D001921:Brain / Q000378:metabolism; D002498:Centrifugation; D005786:Gene Expression Regulation; D018471:Hypergravity; D007375:Interleukin-1 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha