Identification and characterization of a functionally distinct form of human estrogen receptor beta.
Estrogen receptors are important for the development and maintenance of many different tissues in the body including the breast, uterus, brain and bone. There are two known genes encoding estrogen receptors, Estrogen Receptor alpha (ER alpha) and Estrogen Receptor beta (ER beta). These receptors are transcription factors with distinct functional domains involved in DNA binding, ligand binding and transcriptional regulation. A novel isoform of human ER beta (ER beta 548) which includes an extended amino terminal domain has been identified. Isoform specific antibodies confirm the presence of this receptor in human tissue. Transactivation analysis with different estrogenic ligands indicates that ER beta 548 is functionally distinct from previously reported forms of ER beta.
Wilkinson
Hilary A
HA
Department of Atherosclerosis and Endocrinology, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
Dahllund
Johanna
J
Liu
Hao
H
Yudkovitz
Joel
J
Cai
Sheng-Jian
SJ
Nilsson
Stefan
S
Schaeffer
James M
JM
Mitra
Sudha W
SW
D015153:Blotting, Western; D003062:Codon / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D047629:Estrogen Receptor beta; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007536:Isomerism; D008297:Male; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism; D015533:Transcriptional Activation
D003062:Codon; D017931:DNA Primers; D018076:DNA, Complementary; D047629:Estrogen Receptor beta; D011960:Receptors, Estrogen