Mass spectrometric separation and determination of N1,N12-diacetylspermine in the urine of cancer patients.
An ionspray ionization mass-spectrometric method for the determination of N1,N12-diacetylspermine (Ac2Spm) was developed using 15N-labeled Ac2Spm as the internal standard. Concentrations of Ac2Spm in the urine obtained from 17 cancer patients measured by the present method correlated well with those measured by ELISA, showing the usefulness of the two methods.
Kobayashi
Masaki
M
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Samejima
Keijiro
K
Hiramatsu
Kyoko
K
Kawakita
Masao
M
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009369:Neoplasms / Q000652:urine*; D012015:Reference Standards; D013096:Spermine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine*
D013096:Spermine; C029453:N',N''-diacetylspermine