Identification of lactoferrin-binding proteins in Streptococcus dysgalactiae subsp. dysgalactiae and Streptococcus agalactiae isolated from cows with mastitis.
Three strains of Streptococcus dysgalactiae subsp. dysgalactiae (S. dysgalactiae) and five strains of Streptococcus agalactiae were used to identify lactoferrin-binding proteins (LBPs). LBPs from extracted surface proteins were detected by polyacrylamide gel electrophoresis and Western blotting. All strains of S. dysgalactiae evaluated had 52- and 74-kDa protein bands. All strains of S. agalactiae evaluated had 52-, 70- and 110-kDa protein bands. In addition, a 45-kDa band was detected in two of five S. agalactiae strains evaluated. This study demonstrated that S. dysgalactiae and S. agalactiae of bovine origin contain two and three major LBPs, respectively.
Park
Hee-Myung
HM
Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USA.
Almeida
Raul A
RA
Oliver
Stephen P
SP
D000818:Animals; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D008414:Mastitis, Bovine / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification / Q000378:metabolism*; D013292:Streptococcus agalactiae / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; C027768:lactoferrin binding proteins, bacterial