title: Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA.
abstract: Heat-induced formation of 8-oxoguanine was demonstrated in DNA solutions in 10(-3) M phosphate buffer, pH 6.8, by enzyme-linked immunosorbent assays using monoclonal antibodies against 8-oxoguanine. A radiation-chemical yield of 3.7 x 10(-2) micromol x J(-1) for 8-oxoguanine production in DNA upon gamma-irradiation was used as an adequate standard for quantitation of 8-oxoguanine in whole DNA. The initial yield of heat-induced 8-oxoguanine exhibits first order kinetics. The rate constants for 8-oxoguanine formation were determined at elevated temperatures; the activation energy was found to be 27 +/- 2 kcal/mol. Extrapolation to 37 degrees C gave a value of k37 = 4.7 x 10(-10) x s(-1). Heat-induced 8-oxoguanine formation and depurination of guanine and adenine show similarities of the processes, which implies that heat-mediated generation of reactive oxygen species (ROS) should occur. Heat-induced production of H2O2 in phosphate buffer was shown. The sequence of reactions of thermally mediated ROS formation have been established: activation of dissolved oxygen to the singlet state, generation of superoxide radicals and their dismutation to H2O2. Gas saturation (O2, N2 and Ar), D2O, scavengers of 1O2, O2-* and OH* radicals and metal chelators influenced heat-induced 8-oxoguanine formation as they affected thermal ROS generation. These findings imply that heat acts via ROS attack leading to oxidative damage to DNA.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russian Federation. bruskov_v2000@mail.ru
authors.forename: Vadim I
authors.initials: VI
authors.lastname: Bruskov
authors.forename: Lyudmila V
authors.initials: LV
authors.lastname: Malakhova
authors.forename: Zhaksylyk K
authors.initials: ZK
authors.lastname: Masalimov
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Chernikov
mesh_terms: D000225:Adenine / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D004247:DNA / Q000378:metabolism; D004249:DNA Damage; D006147:Guanine / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000378:metabolism; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D006861:Hydrogen Peroxide; D000225:Adenine