Mammalian germ-line transgenesis by transposition.
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
Dupuy
Adam J
AJ
The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology, and Development, University of Minnesota Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
Clark
Karl
K
Carlson
Corey M
CM
Fritz
Sabine
S
Davidson
Ann E
AE
Markley
Karra M
KM
Finley
Ken
K
Fletcher
Colin F
CF
Ekker
Stephen C
SC
Hackett
Perry B
PB
Horn
Sandra
S
Largaespada
David A
DA
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D019895:Transposases / Q000235:genetics / Q000502:physiology
D004251:DNA Transposable Elements; D019895:Transposases