Basolateral, but not apical, ATP inhibits vasopressin action in rat inner medullary collecting duct.
Previous studies have shown that basolateral ATP inhibits vasopressin action in the renal collecting tubule. Although there is evidence for an apical P2Y2 receptor in this tubule segment, it is not known whether apical ATP has similar effects. In the rat inner medullary collecting duct basolateral, but not apical, ATP (0.1-100 microM) reversibly inhibited vasopressin-induced increases in water permeability with an IC50 of 1.09 microM. Basolateral UTP, but not ADP, alpha,beta-methylene-ATP or 2-methylthio-ATP also inhibited vasopressin action. It is concluded that basolateral but not apical P2Y2 receptors inhibit vasopressin action in the collecting duct.
Edwards
Richard M
RM
Department of Renal/Urology Biology, UW2521, GlaxoSmithKline, P.O. Box 1539, King of Prussia, PA 19406-0939, USA. richard_m_edwards@gsk.com
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000187:drug effects* / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009995:Osmosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013873:Thionucleotides / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*
D013873:Thionucleotides; D014867:Water; D014667:Vasopressins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D014544:Uridine Triphosphate; C008501:2-methylthio-ATP