Mechanisms of resistance to organophosphates in Tetranychus urticae (Acari: Tetranychidae) from Greece.
We investigated the mechanisms conferring resistance to methyl-parathion (44-fold) and to methomyl (8-fold) in Tetranychus urticae from Greece by studying the effect of synergists on the resistance and the kinetic characteristics of various enzymes in a resistant strain (RLAB) and a susceptible reference strain (SAMB). It is shown that S,S,S-tributyl phosphorotrithioate, a synergist that inhibits esterases and glutathione S-transferases, and piperonyl butoxide, a synergist that inhibits cytochrome P450 mediated monooxygenases, did not affect the level of methyl-parathion or methomyl resistance in RLAB and that resistance ratios to both insecticides did not change significantly in the presence of either synergist. Isoelectric focusing of esterase allozymes on single mites revealed no differences in staining intensity and glutathione S-transferase activity was not significantly different in the two strains. The activity of two cytochrome P450 monooxygenase groups was compared. No significant difference of 7-ethoxyresorufin-O-diethylase activity was observed between strains that were two-fold higher in RLAB than in SAMB. The kinetic characteristics of acetylcholinesterase, the target enzyme of organophosphates and carbamates, revealed that acetylcholinesterase in RLAB was less sensitive to inhibition by paraoxon and methomyl in comparison with SAMB. I(50), the inhibitor concentration inducing 50% decrease of acetylcholinesterase activity was greater (119- and 50-fold with paraoxon and methomyl, respectively) and the bimolecular constant k(i) was lower (39- and 47-fold with paraoxon and methomyl, respectively) in RLAB compared to SAMB.
Tsagkarakou
A
A
Institut des Sciences de l'Evolution, Laboratoire Génétique et Environnement, cc 065, Université Montpellier II, 34095 05, Montpellier, France.
Pasteur
N
N
Cuany
A
A
Chevillon
C
C
Navajas
M
M
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D065607:Cytochrome P-450 Enzyme Inhibitors; D004950:Esterases / Q000037:antagonists & inhibitors; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors; D006115:Greece; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008724:Methomyl / Q000494:pharmacology; D008743:Methyl Parathion / Q000494:pharmacology*; D008925:Mites / Q000187:drug effects* / Q000201:enzymology; D010574:Pesticide Synergists / Q000494:pharmacology; D010882:Piperonyl Butoxide / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D065607:Cytochrome P-450 Enzyme Inhibitors; D007306:Insecticides; D010574:Pesticide Synergists; D008724:Methomyl; D008743:Methyl Parathion; D005982:Glutathione Transferase; D004950:Esterases; D000110:Acetylcholinesterase; D010882:Piperonyl Butoxide