Nanobeads as a solid phase in a solute homogeneous assay format.
The measurement of numerous samples as in drug screening or diagnostics has been improved significantly over recent years. The processing of a great number of carriers with 96, 384 or 1536 wells is not the limiting step anymore and more than 100,000 samples can be analyzed within 24 h. New challenges arise in optimizing data quality and assay volume in order to gain more reliable results with a reduced consumption of assay reagents. Both are addressed with an approach using dispersed nanoparticles analyzed by a new detection technology, fluorescence intensity distribution analysis. This homogeneous assay is a generic format holding great potential in expanding the assay strategy portfolio for diagnostic and screening applications.
Schaertl
S
S
Evotec OAI AG, Schnackenburgallee 114, 22525 Hamburg, Germany. sabine.schaertl@evotecoai.com
Meyer-Almes
F J
FJ
D001331:Automation; D001665:Binding Sites; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D006801:Humans; D008024:Ligands; D008027:Light; D025202:Molecular Diagnostic Techniques; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence
D008024:Ligands