title: Immunization with recombinant modified vaccinia virus Ankara can modify mucosal simian immunodeficiency virus infection and delay disease progression in macaques.
abstract: In the present study, the immunogenicity and protective efficacy of a recombinant vaccinia virus-based simian immunodeficiency virus (SIV) vaccine, given alone or in combination with a protein boost, were investigated. Cynomolgus macaques were immunized intramuscularly with modified vaccinia virus Ankara (MVA) expressing the SIVsm env and gag-pol genes (MVA-SIVsm) at 0 and 3 months (n=4), at 0, 3 and 8 months (n=4) or at 0 and 3 months followed by purified native SIVsm gp148 and recombinant SIVmac p27 in immunostimulatory complexes at 8 months (n=4). One month after the last immunization, the vaccinees, together with four naive control monkeys and four monkeys immunized with wild-type MVA, were challenged intrarectally with 10 MID50 SIVsm. At the time of challenge, antibody titres to SIV Env and lymphocyte proliferation responses to whole viral antigen were highest in vaccinees receiving MVA-SIVsm in combination with protein immunizations. Following rectal challenge, one of these vaccinees was completely protected. A prolonged survival time was observed in two of four monkeys in each of the groups immunized with MVA-SIVsm, in two monkeys given MVA-SIVsm followed by protein and in three of four monkeys given wild-type MVA, compared with naive controls. In conclusion, one monkey given the combined vaccine was protected completely against SIVsm infection. Furthermore, immunization with MVA-SIVsm, as well as wild-type MVA alone, seemed to delay disease progression after mucosal SIV infection in a proportion of the monkeys.
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82 Solna, Sweden1.
authors.forename: Charlotta
authors.initials: C
authors.lastname: Nilsson
authors.affiliation: Institute for Molecular Virology, GSF - National Research Centre for Environment and Health, Trogerstr. 4b, 81675 Munich, Germany2.
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82 Solna, Sweden1.
authors.forename: Lilian
authors.initials: L
authors.lastname: Walther-Jallow
authors.affiliation: Department of Virology, Biomedical Primate Research Centre, 2280 GH Rijswijk, The Netherlands3.
authors.forename: Peter
authors.initials: P
authors.lastname: Ten Haaft
authors.affiliation: Department of Virology, The National Veterinary Institute, Uppsala, Sweden4.
authors.forename: Lennart
authors.initials: L
authors.lastname: Åkerblom
authors.affiliation: Department of Virology, Biomedical Primate Research Centre, 2280 GH Rijswijk, The Netherlands3.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Heeney
authors.affiliation: Institute for Molecular Virology, GSF - National Research Centre for Environment and Health, Trogerstr. 4b, 81675 Munich, Germany2.
authors.forename: Volker
authors.initials: V
authors.lastname: Erfle
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82 Solna, Sweden1.
authors.forename: Per
authors.initials: P
authors.lastname: Böttiger
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82 Solna, Sweden1.
authors.forename: Gunnel
authors.initials: G
authors.lastname: Biberfeld
authors.affiliation: Swedish Institute for Infectious Disease Control and Microbiology and Tumor Biology Center, Karolinska Institute, SE-171 82 Solna, Sweden1.
authors.forename: Rigmor
authors.initials: R
authors.lastname: Thorstensson
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D007114:Immunization; D008252:Macaca fascicularis; D018449:SAIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D018449:SAIDS Vaccines; D014614:Vaccines, Synthetic