title: First description of Klebsiella pneumoniae harboring CTX-M beta-lactamases (CTX-M-14 and CTX-M-3) in Taiwan.
abstract: Klebsiella pneumoniae isolates from Taiwan medical centers (50 strains; 1998 to 2000) with a CTX-M resistance phenotype (ceftazidime susceptible and ceftriaxone or cefotaxime nonsusceptible) were selected for initial isoelectric focusing analysis. beta-Lactamases with pIs of 7.9 (n = 22) and 8.4 (n = 28) in addition to 5.4 and/or 7.6 were detected. DNA gene sequencing identified the beta-lactamases with pIs of 7.9 and 8.4 as CTX-M-14 and CTX-M-3, respectively. Molecular typing suggested inter- and intrahospital clonal dissemination of these Taiwanese CTX-M-producing Klebsiella strains.
authors.affiliation: University of Iowa College of Medicine, Iowa City, USA. .
authors.forename: Wen Liang
authors.initials: WL
authors.lastname: Yu
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Winokur
authors.forename: Diana L
authors.initials: DL
authors.lastname: Von Stein
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: Jen Hsien
authors.initials: JH
authors.lastname: Wang
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Jones
mesh_terms: D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan / Q000453:epidemiology; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; C456080:beta-lactamase CTX-M-14; C417271:beta-lactamase CTX-M-3; D001618:beta-Lactamases