Cross-reactivity and sensitivity of two Legionella urinary antigen kits, Biotest EIA and Binax NOW, to extracted antigens from various serogroups of L. pneumophila and other Legionella species.
Legionella antigen detection kits for diagnosing legionellosis from urine have become widely used, but basic information about reactivity of the kits to non-serogroup (SG) 1 L. pneumophila and other Legionella species remains incomplete. We evaluated Biotest EIA and the most recently developed Binax NOW by using in-vitro extracted antigens of 22 L. pneumophila SG 1 to 15 strains and of 27 other Legionella species. Both kits showed excellent sensitivity to L pneumophila SG 1 antigens, but reacted to different sets of non-SG I L. pneumophila with different sensitivity. No cross-reactivity was observed to Legionella species other than L. pneumophila.
Okada
Chikako
C
Laboratory of Clinical Microbiology, Toranomon Hospital, Tokyo, Japan.
Kura
Fumiaki
F
Wada
Akihito
A
Inagawa
Hiroko
H
Lee
Gang-Hong
GH
Matsushita
Hiroshi
H
D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification / Q000652:urine*; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000145:classification / Q000276:immunology*; D016952:Legionella pneumophila / Q000145:classification / Q000276:immunology; D007876:Legionellosis / Q000175:diagnosis*; D007877:Legionnaires' Disease / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012703:Serotyping
D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic