Immunization of sheep against Fasciola gigantica with glutathione S-transferase.
Glutathione S-transferase in Fasciola gigantica (FgGST) was isolated by affinity chromatography, by which highly purified enzyme was obtained. FgGST on the SDS-PAGE showed three protein bands ranging 24.5-26.5kDa. Glutathione S-transferase (GST) activity was determined by HABIG method. FgGST was evaluated as vaccine alone or in combination with either aluminum hydroxide or saponin in sheep against F. gigantica infection. ELISA was used for detection of anti-FgGST IgG. After vaccination, all sheep were challenged with 120 metacercaria of F. gigantica. The results indicated that anti-GST IgG was not elevated after challenge. All sheep were slaughtered 24-26 weeks after challenge. The results indicated that, although after second vaccination, antibody titers rose markedly in GST-Al(OH)(3) and GST-saponin groups, but declined 4 weeks after challenge. No correlation between anti-GST IgG titers and protection was observed. The highest fluke burden reduction was observed in the group vaccinated with GST-saponin (32%), but this reduction was not statistically significant in comparison with the control group.
Paykari
H
H
Razi Vaccine and Serum Research Institute, Karaj, Iran. payk@rvsri.com
Dalimi
A
A
Madani
R
R
D000536:Aluminum Hydroxide; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D002846:Chromatography, Affinity / Q000379:methods / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005209:Fasciola / Q000201:enzymology / Q000276:immunology*; D005211:Fascioliasis / Q000517:prevention & control / Q000662:veterinary*; D005982:Glutathione Transferase / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D012503:Saponins; D012756:Sheep; D012757:Sheep Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods / Q000662:veterinary*
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D012503:Saponins; D000536:Aluminum Hydroxide; D005982:Glutathione Transferase