title: Increased proliferation of neural progenitor cells but reduced survival of newborn cells in the contralateral hippocampus after focal cerebral ischemia in rats.
abstract: Recent studies demonstrated that neurogenesis in the adult hippocampus increased after transient global ischemia; however, the molecular mechanism underlying increased neurogenesis after ischemia remains unclear. The finding that proliferation of progenitor cells occurred at least a week after ischemic insult suggests that the stimulus was not an ischemic insult to progenitor cells. To clarify whether focal ischemia increases the rate of neurogenesis in the remote area, the authors examined the contralateral hemisphere in rats subjected to permanent occlusion of the middle cerebral artery. In the subgranular zone of the hippocampal dentate gyrus, the numbers of bromodeoxyuridine (BrdU)-positive cells increased approximately sixfold 7 days after ischemia. In double immunofluorescence staining, more than 80% of newborn cells expressed Musashi1, a marker of neural stem/progenitor cells, but only approximately 10% of BrdU-positive cells expressed glial fibrillary acidic protein (GFAP), a marker of astrocytes. The number of BrdU-positive cells markedly decreased 28 days after BrdU administration after ischemia, but it was still elevated compared with that of sham-operated rats. In double immunofluorescence staining, 80% of newborn cells expressed NeuN, a marker of differentiated neurons, and 10% of BrdU-positive cells expressed GFAP. However, in the other areas of the contralateral hemisphere including the rostral subventricular zone, the number of BrdU-positive cells remained unchanged. These results showed that focal ischemia stimulated the proliferation of neuronal progenitor cells, but did not support survival of newborn cells in the contralateral hippocampus.
authors.affiliation: Division of Strokology, Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Japan.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Takasawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yagita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Sasaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tanaka
authors.forename: Kohji
authors.initials: K
authors.lastname: Matsushita
authors.forename: Toshiho
authors.initials: T
authors.lastname: Ohstuki
authors.forename: Takaki
authors.initials: T
authors.lastname: Miyata
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Hori
authors.forename: Masayasu
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology; D002470:Cell Survival; D018891:Dentate Gyrus / Q000473:pathology*; D007839:Functional Laterality; D006624:Hippocampus / Q000473:pathology*; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D020768:Middle Cerebral Artery; D009474:Neurons / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000473:pathology*