title: Phosphatidylglycerol requirement for the function of electron acceptor plastoquinone Q(B) in the photosystem II reaction center.
abstract: Phosphatidylglycerol (PG), a ubiquitous constituent of thylakoid membranes of chloroplasts and cyanobacteria, is demonstrated to be essential for the functionality of plastoquinone electron acceptor Q(B) in the photosystem II reaction center of oxygenic photosynthesis. Growth of the pgsA mutant cells of Synechocystis sp. PCC6803 that are defective in phosphatidylglycerolphosphate synthase and are incapable of synthesizing PG, in a medium without PG, resulted in a 90% decrease in PG content and a 50% loss of photosynthetic oxygen-evolving activity as reported [Hagio, M., Gombos, Z., Várkonyi, Z., Masamoto, K., Sato, N., Tsuzuki, M., and Wada, H. (2000) Plant Physiol. 124, 795-804]. We have studied each step of the electron transport in photosystem II of the pgsA mutant to clarify the functional site of PG. Accumulation of Q(A)(-) was indicated by the fast rise of chlorophyll fluorescence yield under continuous and flash illumination. Oxidation of Q(A)(-) by Q(B) plastoquinone was shown to become slow, and Q(A)(-) reoxidation required a few seconds when measured by double flash fluorescence measurements. Thermoluminescence measurements further indicated the accumulation of the S(2)Q(A)(-) state but not of the S(2)Q(B)(-) state following the PG deprivation. These results suggest that the function of Q(B) plastoquinone was inactivated by the PG deprivation. We assume that PG is an indispensable component of the photosystem II reaction center complex to maintain the structural integrity of the Q(B)-binding site. These findings provide the first clear identification of a specific functional site of PG in the photosynthetic reaction center.
authors.affiliation: Department of Biology, Faculty of Sciences, Kyushu University, Ropponmatsu, Fukuoka 810-8560, Japan.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Gombos
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Várkonyi
authors.forename: Miki
authors.initials: M
authors.lastname: Hagio
authors.forename: Masayo
authors.initials: M
authors.lastname: Iwaki
authors.forename: László
authors.initials: L
authors.lastname: Kovács
authors.forename: Kazumori
authors.initials: K
authors.lastname: Masamoto
authors.forename: Shigeru
authors.initials: S
authors.lastname: Itoh
authors.forename: Hajime
authors.initials: H
authors.lastname: Wada
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism; D004583:Electrons; D005453:Fluorescence; D010100:Oxygen / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000187:drug effects / Q000378:metabolism*; D010971:Plastoquinone / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology
chemical_list: D010715:Phosphatidylglycerols; D045322:Photosynthetic Reaction Center Complex Proteins; D011809:Quinones; D002734:Chlorophyll; D010971:Plastoquinone; D010100:Oxygen