title: [Studies on bioconversion conditions and stereoselectivity of Arthrobacter K1108 hydantoinase].
abstract: The bioconversion conditions of hydantoinase of Arthrobacter K1108 were studied. It was shown that the optimum temperature of the enzyme is 55 degrees C, and the optimum pH is 7.0. The enzyme can be activated by Co2+ and Fe2+, while inhibited by Ca2+. The optimal substrate of the hydantoinase is 5-benzylhydantoin, while 5-phenylhydantoin and 5-indolylmethylhydantoin cannot effectively digested, showing a high specificity on the substrates. An investigation on the hydantoinase stereoselectivity mechanism showed that the N-carbamoylamino acid hydrolase is stereoselective but the hydantoin hydrolase is not.
authors.affiliation: Institute of Ecology, Chinese Academy of Science, Shenyang 110015, China. zhangwc@nic.bmi.ac.cn
authors.forename: W C
authors.initials: WC
authors.lastname: Zhang
authors.forename: H J
authors.initials: HJ
authors.lastname: Yuan
authors.forename: S F
authors.initials: SF
authors.lastname: Hao
authors.forename: Q Z
authors.initials: QZ
authors.lastname: Peng
authors.forename: S J
authors.initials: SJ
authors.lastname: Wang
authors.forename: H C
authors.initials: HC
authors.lastname: Zhu
authors.forename: L Y
authors.initials: LY
authors.lastname: Huang
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D001173:Arthrobacter / Q000201:enzymology*; D001711:Biotransformation; D004789:Enzyme Activation; D006827:Hydantoins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D006827:Hydantoins; C031427:5-benzylhydantoin; D000581:Amidohydrolases; C020047:dihydropyrimidinase