title: Radiation responsiveness of human lung cancer cell lines measured with a short term semiautomatic assay.
abstract: BACKGROUND
  Fluorometric microculture cytotoxicity assay (FMCA) is a short-term semi-automatic method, based on dye-inclusion of surviving cells. The assay was developed for investigations of drug resistance on tumour cells from biopsy material. In the present study, this short-term assay was evaluated, regarding usefulness in determining radio-sensitivity.
  
  
  METHODS
  Eight human lung cancer cell lines were used. There were five small cell lung cancer (SCLC and three non-small cell lung cancer (NSCLC cell lines. Results were compared with the corresponding data derived from the clonogenic assay and/or the extrapolation method.
  
  
  RESULTS
  The surviving fraction (SF) after 2, 5 and 10 Gy compared with data from the clonogenic assay were not in accordance for 5 of the 8 cell lines. The FMCA assay overestimated SF- values for the SCLC cell lines.
  
  
  CONCLUSIONS
  The FMCA assay is not useful as a quick screening method for the radioresponsiveness in vitro of human tumour cell lines.
authors.affiliation: Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Xing
authors.forename: D
authors.initials: D
authors.lastname: Brattstrom
authors.forename: M
authors.initials: M
authors.lastname: Bergqvist
authors.forename: U
authors.initials: U
authors.lastname: Isaksson
authors.forename: G
authors.initials: G
authors.lastname: Wagenius
authors.forename: O
authors.initials: O
authors.lastname: Brodin
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000532:radiotherapy*; D018288:Carcinoma, Small Cell / Q000473:pathology / Q000532:radiotherapy*; D002455:Cell Division / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured