Peripheral T-cell lymphoma in herpesvirus saimiri-infected tamarins: tumor cell lines reveal subgroup-specific differences.
Efficiency of lymphoma induction by herpesvirus saimiri (HVS) isolates correlates with the genetically defined viral subgroups A, B, and C. To compare subgroup-specific effects, highly susceptible tamarins were infected with HVS strain A-11, B-SMHI, or C-488. All animals developed T-cell lymphomas indistinguishable with respect to clinical, pathological, and virological parameters. Ex vivo T-cell lines were established readily from the HVS C-488 animal, less efficiently in the presence of HVS A-11, and from only a single HVS B-SMHI sample. These cultivated cells revealed strain-specific biochemical characteristics. HVS A-11 strongly induced the expression of tyrosine kinase Lyn. HVS C-488 led to the activation of STAT3, which is most likely linked to the association of virus-encoded Tip with tyrosine kinase Lck. The lack of these activities in HVS B-SMHI-transformed cells may correlate with the reduced oncogenic phenotype of this virus in species other than tamarins.
Reiss
Christine
C
Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Niedobitek
Gerald
G
Hör
Simon
S
Lisner
Renate
R
Friedrich
Ute
U
Bodemer
Walter
W
Biesinger
Brigitte
B
D000818:Animals; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism; D006566:Herpesviridae Infections / Q000473:pathology / Q000821:virology*; D006567:Herpesvirus 2, Saimiriine / Q000145:classification / Q000472:pathogenicity* / Q000502:physiology; D016399:Lymphoma, T-Cell / Q000473:pathology / Q000821:virology*; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D050796:STAT3 Transcription Factor; D012452:Saguinus; D013601:T-Lymphocytes / Q000821:virology; D015534:Trans-Activators / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*; D014764:Viral Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D050796:STAT3 Transcription Factor; C064364:STP protein, Herpesvirus saimiri; D015534:Trans-Activators; D014764:Viral Proteins; C092384:tyrosine kinase interacting protein, Saimiriine herpesvirus 2; D011505:Protein-Tyrosine Kinases