title: Cellular cardiomyoplasty in a transgenic mouse model.
abstract: BACKGROUND
  Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality.
  
  
  METHODS
  Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter.
  
  
  RESULTS
  The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals.
  
  
  CONCLUSIONS
  This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.
authors.affiliation: Department of Cardiac Surgery, University of Bonn, 53105 Bonn, Germany. akp44@uni-koeln.de.
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Roell
authors.forename: Yun
authors.initials: Y
authors.lastname: Fan
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
authors.forename: Eva
authors.initials: E
authors.lastname: Stoecker
authors.forename: Philipp
authors.initials: P
authors.lastname: Sasse
authors.forename: Eugen
authors.initials: E
authors.lastname: Kolossov
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Bloch
authors.forename: Harald
authors.initials: H
authors.lastname: Metzner
authors.forename: Christoph
authors.initials: C
authors.lastname: Schmitz
authors.forename: Klaus
authors.initials: K
authors.lastname: Addicks
authors.forename: Juergen
authors.initials: J
authors.lastname: Hescheler
authors.forename: Armin
authors.initials: A
authors.lastname: Welz
authors.forename: Bernd K
authors.initials: BK
authors.lastname: Fleischmann
mesh_terms: D000818:Animals; D018421:Cardiomyoplasty / Q000379:methods*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins