title: Novel immediate-early protein IE19 of human cytomegalovirus activates the origin recognition complex I promoter in a cooperative manner with IE72.
abstract: The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequencing revealed that the 0.65-kb cDNA is from exons 1, 2, and 3 and part of exon 4, indicating that it is derived from a novel alternatively spliced mRNA of the MIE gene. The cDNA encodes a 172-amino-acid polypeptide, termed IE19, which corresponds to an IE72 variant with an internal deletion from Val(86) to Pro(404) and appears as a band at 38 kDa on a sodium dodecyl sulfate-polyacrylamide gel. IE19 mRNA was expressed at a low level in the immediate-early, early, and late period of viral infection. IE19 was localized in nuclei, and a transient-expression assay revealed that IE19 enhances IE72-dependent activation of the HsOrc1 promoter, which is identified here as an IE72 target promoter. Another MIE protein, IE86, activated the same promoter but only weakly compared to IE72, and coexpression of IE19 did not alter the IE86-mediated transcriptional activation. In addition, IE19 did not enhance the IE72-dependent activation of the HCMV UL54 promoter. These results suggest that IE19 is a transcriptional coactivator that works with IE72.
authors.affiliation: Department of Tumor Virology, Division of Virology and Immunology, Medical Research Institute, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo, Tokyo 113-8510, Japan. shirakata.creg@mri.tmd.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Shirakata
authors.forename: Masakazu
authors.initials: M
authors.lastname: Terauchi
authors.forename: Melike
authors.initials: M
authors.lastname: Ablikim
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Imadome
authors.forename: Kanji
authors.initials: K
authors.lastname: Hirai
authors.forename: Takeshi
authors.initials: T
authors.lastname: Aso
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamanashi
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D003587:Cytomegalovirus / Q000737:chemistry / Q000502:physiology*; D004261:DNA Replication; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051738:Origin Recognition Complex; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C088645:IE1 protein, cytomegalovirus; C451749:IE19 protein, human cytomegalovirus; D017874:Immediate-Early Proteins; D051738:Origin Recognition Complex; D012333:RNA, Messenger; D014764:Viral Proteins