Heat shock cognate protein 70 is involved in rotavirus cell entry.
In this work, we have identified the heat shock cognate protein (hsc70) as a receptor candidate for rotaviruses. hsc70 was shown to be present on the surface of MA104 cells, and antibodies to this protein blocked rotavirus infectivity, while not affecting the infectivity of reovirus and poliovirus. Preincubation of the hsc70 protein with the viruses also inhibited their infectivity. Triple-layered particles (mature virions), but not double-layered particles, bound hsc70 in a solid-phase assay, and this interaction was blocked by monoclonal antibodies to the virus surface proteins VP4 and VP7. Rotaviruses were shown to interact with hsc70 at a postattachment step, since antibodies to hsc70 and the protein itself did not inhibit the virus attachment to cells. We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70.
Guerrero
Carlos A
CA
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico, USA.
Bouyssounade
Daniela
D
Zárate
Selene
S
Isa
Pavel
P
López
Tomás
T
Espinosa
Rafaela
R
Romero
Pedro
P
Méndez
Ernesto
E
López
Susana
S
Arias
Carlos F
CF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D011991:Receptors, Virus / Q000378:metabolism; D012401:Rotavirus / Q000378:metabolism / Q000472:pathogenicity*; D012400:Rotavirus Infections / Q000821:virology*
D000911:Antibodies, Monoclonal; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D011991:Receptors, Virus