Adenovirus cores can function as templates in in vitro DNA replication.
Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein-dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA-protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.
Goding
C R
CR
Division of Virology, National Institute for Medical Research, London, UK.
Russell
W C
WC
D000260:Adenoviruses, Human / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D003843:Deoxycytidine Monophosphate / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D009675:Novobiocin / Q000494:pharmacology; D010750:Phosphoproteins / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D013698:Templates, Genetic; D059005:Topoisomerase II Inhibitors; D014758:Viral Core Proteins / Q000378:metabolism*; D014764:Viral Proteins
D004279:DNA, Viral; D004791:Enzyme Inhibitors; D010750:Phosphoproteins; D011498:Protein Precursors; D059005:Topoisomerase II Inhibitors; D014758:Viral Core Proteins; D014764:Viral Proteins; C085476:pTP protein, adenovirus; D003843:Deoxycytidine Monophosphate; D009675:Novobiocin