title: Torsional stress induces left-handed helical stretches in DNA of natural base sequence: circular dichroism and antibody binding.
abstract: Above a threshold of torsional stress, the c.d. spectrum of covalently closed circular DNA of natural base sequence acquires a Z-like contribution and antibodies raised against Z-DNA are bound. Mapping of the antibody binding sites by electron microscopy reveals sites which correlate with stretches enriched in alternating purine-pyrimidine sequences and GC base pairs.
authors.affiliation: Institut für Zellbiologie, ETH, Hönggerberg, Zürich, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Di Capua
authors.forename: A
authors.initials: A
authors.lastname: Stasiak
authors.forename: T
authors.initials: T
authors.lastname: Koller
authors.forename: S
authors.initials: S
authors.lastname: Brahms
authors.forename: R
authors.initials: R
authors.lastname: Thomae
authors.forename: F M
authors.initials: FM
authors.lastname: Pohl
mesh_terms: D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004270:DNA, Circular / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D005914:Globins / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D011817:Rabbits; D013314:Stress, Mechanical
chemical_list: D000906:Antibodies; D004270:DNA, Circular; D005914:Globins