Characterization of an alanine racemase gene from Lactobacillus reuteri.
An alanine racemase gene from Lb. reuteri was cloned by using degenerate oligonucleotides corresponding to conserved regions derived from several bacterial alanine racemases. The protein is 375alphaalpha in length and shows 63.6% homology to the Lb. plantarum alanine racemase. Unlike the single alanine racemase activity found in Lb. plantarum, deletion of the Lb. reuteri alanine racemase reveals a second activity, which is inhibited by beta-chloro- D-alanine.
Thompson
Arthur
A
Institute of Food Research, Colney Lane, Colney, Norwich, NR4 7UA, UK. arthur.thompson@bbsrc.ac.uk
Griffin
Hugh
H
Gasson
Michael J
MJ
D000411:Alanine Racemase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017353:Gene Deletion; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology
D004791:Enzyme Inhibitors; D015091:beta-Alanine; C006977:3-chloroalanine; D000411:Alanine Racemase