title: Selection of Gongronella butleri strains for enhanced chitosan yield with UV mutagenesis.
abstract: This paper describes the selection of Gongronella butleri strains producing higher chitosan yield using UV mutagenesis. We have devised an enzyme-linked immunosorbent assay for the selection of high chitin deacetylase (CDA) yielding strains. Mutant strains M+1, M+2 and M+7 could produce twice the extractable chitosan yield and double the CDA activity, as compared to the wild type strain.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Lower Kent Ridge Road, 117543, Singapore, Singapore
authors.forename: Theingi
authors.initials: T
authors.lastname: Maw
authors.forename: Teck Koon
authors.initials: TK
authors.lastname: Tan
authors.forename: Eugene
authors.initials: E
authors.lastname: Khor
authors.forename: Sek Man
authors.initials: SM
authors.lastname: Wong
mesh_terms: D000581:Amidohydrolases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000906:Antibodies; D000941:Antigens / Q000032:analysis; D001709:Biotechnology; D002686:Chitin / Q000031:analogs & derivatives / Q000096:biosynthesis*; D048271:Chitosan; D004797:Enzyme-Linked Immunosorbent Assay; D009090:Mucorales / Q000201:enzymology* / Q000235:genetics*; D016296:Mutagenesis; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D000941:Antigens; D002686:Chitin; D048271:Chitosan; D000581:Amidohydrolases; C019866:chitin deacetylase