Universal Linkage System: versatile nucleic acid labeling technique.
Over the last two decades nonradioactive nucleic acid labeling and detection systems have overcome the safety, disposal, stability and cost problems that are associated with radioactive techniques. Besides traditional, enzyme-mediated, nonradioactive labeling methods (e.g., random priming, nick translation or labeling by PCR), several chemical labeling systems have been developed (e.g., ULS, psoralen, alkylating agents). These methods provide fluorescent (or hapten) labeled probes for fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH) and microarray-based techniques. In this review the DNA-based molecular diagnostic applications and perspectives of the Universal Linkage System (ULS) technology will be described.
van Gijlswijk
R P
RP
Kreatech Diagnostics BV, PO Box 37078, 1030 AB Amsterdam, The Netherlands.
Talman
E G
EG
Janssen
P J
PJ
Snoeijers
S S
SS
Killian
J
J
Tanke
H J
HJ
Heetebrij
R J
RJ
D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D025202:Molecular Diagnostic Techniques; D009696:Nucleic Acids / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D013194:Staining and Labeling / Q000379:methods*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification
D015342:DNA Probes; D009696:Nucleic Acids; D012333:RNA, Messenger