title: Mast cells may not play a crucial role in the pathogenesis of experimental closed duodenal loop-induced pancreatitis in rats.
abstract: BACKGROUND
  Ws/Ws rats have a small deletion of the c-kit gene and are deficient in both mucosal-type mast cells and connective tissue-type mast cells.
  
  
  OBJECTIVE
  To investigate the role of pancreatic mast cells in the development of experimental closed duodenal loop (CDL)-induced pancreatitis using Ws/Ws rats.
  
  
  METHODS
  Pancreatitis was induced by the CDL technique for 5 and 12 hours, and the subsequent ascites volume, wet pancreatic weight, pancreatic myeloperoxidase activities, and serum amylase levels were evaluated. The pancreatic tissue damage was also evaluated histologically.
  
  
  RESULTS
  The CDL technique induced equally severe ascites, pancreatic edema and hyperemia, and hyperamylasemia in the Ws/Ws versus the control (+/+) rats. The microscopic mucosal damage score was also equivalent in the Ws/Ws and control (+/+) rats, and there were no significant differences in mucosal myeloperoxidase activity between the Ws/Ws and control (+/+) rats.
  
  
  CONCLUSIONS
  These results indicate that mast cells may not be crucial for the development of CDL-induced pancreatitis.
authors.affiliation: Department of Internal Medicine, Nagahama Red Cross Hospital, Nagahama, Shiga, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Araki
authors.forename: Akira
authors.initials: A
authors.lastname: Andoh
authors.forename: Fumiyasu
authors.initials: F
authors.lastname: Nakamura
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tasaki
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takenaka
authors.forename: Yasunobu
authors.initials: Y
authors.lastname: Komai
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Doi
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujiyama
authors.forename: Tadao
authors.initials: T
authors.lastname: Bamba
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D001201:Ascites / Q000473:pathology; D003250:Constriction; D004195:Disease Models, Animal; D004386:Duodenum / Q000601:surgery*; D004487:Edema / Q000209:etiology; D017353:Gene Deletion; D006940:Hyperemia / Q000209:etiology; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D009092:Mucous Membrane / Q000201:enzymology / Q000473:pathology; D009929:Organ Size; D010179:Pancreas / Q000201:enzymology / Q000473:pathology; D010195:Pancreatitis / Q000209:etiology* / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains; D013997:Time Factors
chemical_list: D009195:Peroxidase; D019009:Proto-Oncogene Proteins c-kit; D000681:Amylases