title: Specific enzyme activities in bronchoalveolar lavage fluid as an aid to diagnosis of tracheobronchitis and bronchopneumonia in dogs.
abstract: Lactate dehydrogenase (LDH), alkaline phosphatase (ALP), alanine aminotransferase and gamma-glutamyl transferase enzyme activities, and total protein (TP), calcium, inorganic phosphate, urea nitrogen (UN) and creatinine concentrations in bronchoalveolar lavage fluid were investigated for their relative importance in the diagnosis of respiratory diseases in dogs. Bronchoalveolar lavage (BAL) fluid was obtained from 26 dogs (20 with respiratory diseases and six controls) following anaesthesia with sodium pentothal. Enzyme activities and biochemical parameters were measured in BAL fluid. LDH and ALP levels were significantly increased in 12 dogs with bronchopneumonia, but not in eight dogs with tracheobronchitis. Insignificant and variable levels of TP and UN concentrations were found in both groups. It was concluded that LDH and ALP enzyme activities could be considered as pointers to pulmonary inflammation and/or damage while TP and UN measurements in BAL fluid may have a place in the identification of changes in respiratory and vascular permeability.
authors.affiliation: University of Selçuk, Faculty of Veterinary Medicine, Department of Internal Medicine, Konya, Turkey. mmaden@selcuk.edu.tr
authors.forename: M
authors.initials: M
authors.lastname: Maden
authors.forename: V
authors.initials: V
authors.lastname: Altunok
authors.forename: F M
authors.initials: FM
authors.lastname: Birdane
authors.forename: V
authors.initials: V
authors.lastname: Aslan
authors.forename: M
authors.initials: M
authors.lastname: Nizamlioğlu
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001806:Blood Urea Nitrogen; D001991:Bronchitis / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001996:Bronchopneumonia / Q000175:diagnosis / Q000201:enzymology / Q000662:veterinary*; D002118:Calcium / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis / Q000201:enzymology*; D004285:Dogs; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D002118:Calcium