[Homoeologous grouping of R. kamoji chromosomes introduced in wheat using RFLP molecular marks].
Twenty six DNA probes from seven homoeologous groups of triticeae were screened to reveal the RFLP between 45 wheat-R. kamoji derivatives and their parents R. kamoji, Chinese Spring, Yangmai 5. The result showed that the introduced R. kamoji chromosomes in 16 wheat-R. kamoji alien chromosome lines including additions, substitution or putative translocations were grouped into homoeologous group 1, 3, 5, 6 and 7. Alien chromosome pairs could be readily transmitted into the descendants. The added chromosomes in K139, K141, K214, K218, K219 and K224 disomic addition lines were grouped into homoeologous group 1, but the added chromosomes in K214 and K218 were different from K219 and K224 which originated from different genomes of R. kamoji. Ditelosomic addition line K147 might involve a R. kamoji chromosome long arm homoeologous to group 1 of wheat, and the added R. kamoji 1 L chromosomes in K139, K141 and K147 probably derived from different three genomes of R. kamoji. U chromosome of R. kamoji. showed homology to wheat homoeologous group 1. Homoeologous group 1 chromosome of R. kamoji, particularly its long arm is related to genes for scab resistance. Result also demonstrated a possible rearrangement occurred between homoeologous group 1 and group 6 of R. kamoji. Two R. kamoji chromosomes introduced in K203 were grouped to homoeologous group 1 and 6, respectively. In K166, the introgressed R. kamoji chromosome involved the short arm of group 5. Another alien chromosome line K177 was revealed as to be with introduced chromosome involving group 5L, 6S and 7SL of R. kamoji. Results also confirmed the homoeology between S, H and Y genomes of R. kamoji.
Wan
Ping
P
Key Lab of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agri. Univ., Nanjing 210095, China.
Wang
Su-Ling
SL
Chen
Pei-Du
PD
Ma
Zheng-Qiang
ZQ
Liu
Da-Jun
DJ
D002678:Chimera / Q000235:genetics*; D002875:Chromosomes; D015342:DNA Probes; D007621:Karyotyping; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D026801:Synteny / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D015342:DNA Probes