title: Effect of perfusion and preservation on IL1-beta, IL-6, and IL-10 production in murine lung.
abstract: We evaluated the production of the interleukins (ILs) IL-1beta, IL-6, and IL-10 in both the vasculature and pulmonary tissue before and after 24 h of lung preservation. The cardiopulmonary blocs of 21 Balb-c mice were divided into three study groups (7 mice/group) and were flushed through the pulmonary artery with Krebs-Henseleit buffer (K-Hb) at 4 degrees C at a rate of 0.2 mL/min as follows: Group 1, lung washout: lungs were flushed until pulmonary effluent was clear. Group 2, perfusion: After lungs were flushed until pulmonary effluent was clear, lungs were perfused during 30 min. Group 3, preservation: Lungs were flushed until pulmonary effluent was clear, and the cardiopulmonary bloc was preserved immersed into (K-Hb) at 4 degrees C. After 24 h of preservation, lungs were reperfused during 30 min. In all study groups the caudal lobe from the left lung was taken for microscopical study; all other lobes were homogenized with (K-Hb) and the supernatant was obtained. IL-1beta, IL-6, and IL-10 production in lung effluents (washout, perfusion, and reperfusion) and in lung tissue were measured by enzyme-linked immunosorbent assay (ELISA). In the lung effluent, there was no statistical difference between IL-1beta and IL-6 concentrations. In all study groups, IL-10 production was significantly higher than IL-1beta and IL-6 levels. IL-10 level was lowest in the 24-h preservation group when it was compared to the other groups. In group 1, there was a negative correlation (r = -.599, p < .05) between IL-1beta and IL-10. In pulmonary tissue, IL-1beta was higher in group 2 when compared to groups 1 (p = .001) and 3 (p = .002), and it was significantly lower in group 3. IL-10 was lower in group 1 when compared to groups 2 (p = .001) and 3 (p = .004). In groups 1 and 2, IL-1beta was significantly higher than IL-6 and IL-10. In group 3, IL-10 was higher than IL-1beta (p = .0001) and IL-6 (p = .0001). Correlation of effluent/tissue index with histological findings showed a negative correlation between IL-10 effluent/tissue relation and inflammation (r = -.68, p < .01). In conclusion, the main cytokine found in lung effluents was IL-10, followed by IL-6 and IL-1beta. On the other hand, cytokine concentration in lung tissue homogenates was mainly due to the presence of IL-1beta. However, this cytokine shows a significant reduction in lung tissue after prolonged preservation.
authors.affiliation: Department of Experimental Surgery, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, México. avesotve@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Sotres-Vega
authors.forename: R
authors.initials: R
authors.lastname: Olmos-Zúñiga
authors.forename: R
authors.initials: R
authors.lastname: Jasso-Victoria
authors.forename: M
authors.initials: M
authors.lastname: Gaxiola-Gaxiola
authors.forename: P
authors.initials: P
authors.lastname: Santillàn-Doherty
mesh_terms: D000818:Animals; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007511:Ischemia / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009926:Organ Preservation; D010477:Perfusion; D013997:Time Factors
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D016753:Interleukin-10