title: The outer membrane fraction of Flavobacterium psychrophilum induces protective immunity in rainbow trout and ayu.
abstract: Flavobacterium psychrophilum is the causative agent of coldwater disease, which is responsible for serious losses in fish aquaculture in several parts of the world. No commercial vaccines are currently available for the prevention of coldwater disease. The present study sought to assess the efficacy of a F. psychrophilum vaccine based on the antigenic outer membrane fraction (OMF). This fraction induced significantly higher protection against coldwater disease in both rainbow trout (Oncorhynchus mykiss) and ayu (Plecoglossus altivelis) compared to inactivated whole cell F. psychrophilum bacterin. Coincident with higher protection, sera of fish immunised with the OMF vaccine had higher agglutination titres than those of fish immunised with inactivated whole cell F. psychrophilum.
authors.affiliation: Immunology Section, National Research Institute of Aquaculture, Fisheries Research Agency, Tamaki, Mie, Japan.
authors.forename: Habibur
authors.initials: H
authors.lastname: Rahman
authors.forename: Akashi
authors.initials: A
authors.lastname: Kuroda
authors.forename: Johannes M
authors.initials: JM
authors.lastname: Dijkstra
authors.forename: Ikunari
authors.initials: I
authors.lastname: Kiryu
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nakanishi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ototake
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015169:Colony Count, Microbial; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D005417:Flavobacterium / Q000737:chemistry / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017686:Oncorhynchus mykiss; D017858:Salmoniformes; D013997:Time Factors; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins