title: Differential expression between pilocytic and anaplastic astrocytomas: identification of apolipoprotein D as a marker for low-grade, non-infiltrating primary CNS neoplasms.
abstract: Fibrillary astrocytoma, the most common primary central nervous system neoplasm, is infiltrating, rapidly proliferating, and almost invariably fatal. This contrasts with the biologically distinct pilocytic astrocytoma, which is circumscribed, often cystic, slowly proliferating, and associated with a favorable long-term outcome. Diagnostic markers for distinguishing pilocytic astrocytomas from infiltrating anaplastic astrocytomas are currently not available. To identify genes that might either serve as markers or explain these distinct biologic behaviors, cDNA microarray analysis was used to compare the expression of 7,073 genes (nearly one quarter of the human genome) between these 2 types of astrocytoma. Messenger RNAs pooled from 3 pilocytic astrocytomas and from 4 infiltrating anaplastic astrocytomas were compared. Apolipoprotein D (apoD), which expressed 8.5-fold higher in pilocytic astrocytomas, showed the greatest level of differential expression and emerged as a potential marker for pilocytic tumors. By immunohistochemistry, 10 of 13 pilocytic astrocytomas stained positively for apoD, while none of 21 infiltrating astrocytomas showed similar staining. ApoD immunostaining was also seen in 9 of 14 of gangliogliomas, 4 of 5 subependymal giant cell astrocytomas (SEGAs), and a single pleomorphic xanthoastrocytomas (PXAs). By in situ hybridization, pilocytic astrocytomas, in contrast with infiltrating astrocytomas, showed widespread increased apoD expression. SAGE analysis using the NCBI database showed a higher level of expression of apoD RNA in pilocytic astrocytoma than in any of the other 94 neoplastic and non-neoplastic tissues in the database. ApoD is associated with decreased proliferation in some cell lines, and is the protein found in highest concentration in cyst fluid from benign cystic disease of the breast. ApoD might play a role in either decreased proliferation or cyst formation in pilocytic astrocytomas, gangliogliomas, SEGAs, and PXAs.
authors.affiliation: Department of Pathology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Hunter
authors.forename: Andrew
authors.initials: A
authors.lastname: Young
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Olson
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Brat
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Bowers
authors.forename: Josiah N
authors.initials: JN
authors.lastname: Wilcox
authors.forename: David
authors.initials: D
authors.lastname: Jaye
authors.forename: Savvas
authors.initials: S
authors.lastname: Mendrinos
authors.forename: Andrew
authors.initials: A
authors.lastname: Neish
mesh_terms: D001053:Apolipoproteins / Q000235:genetics / Q000378:metabolism*; D053399:Apolipoproteins D; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D001053:Apolipoproteins; D053399:Apolipoproteins D; D015415:Biomarkers