Correlations of blood lead with DNA-protein cross-links and sister chromatid exchanges in lead workers.
Levels of sister chromatid exchanges (SCEs), high-SCE frequency cells (HFCs), DNA-protein cross-links (DPCs), blood lead (BLL), and zinc protoporphyrin (ZPP) were measured in peripheral blood from three groups. The lead workers were divided into two groups: a high BLL group (> or =15 microg/dl) and a low BLL group (<15 microg/dl). The control subjects were selected from an area that had not been contaminated with lead and had normal BLL and ZPP levels. In addition, exposure to airborne lead was measured for 11 lead workers, and the time-weighted average was shown to range from 0.19 to 10.32 mg/m(3). The BLL levels of 9 of 11 workers were >15 microg/dl, of which, 3 exceeded current exposure limits (> or =40 microg/dl). The BLL levels of all 11 controls were < 15 microg/dl. The average SCE and DPC values for the workers were 6.1 SCEs/cell and 1.9%, which were significantly higher (P < 0.01, Wilcoxon's test) than the value of 5.2 SCEs/cell and 1.1% for the control subjects. Lead workers had significantly higher BLL and ZPP levels than did the controls. Statistically significant increases in DPCs, SCEs, and HFCs were observed for the high-BLL group compared with the control group. The results of this study suggest that DPCs, SCEs, and HFCs are reliable biomarkers for monitoring workers exposed to lead and clearly indicate health effects from occupational exposure to lead.
Wu
Fang-Yang
FY
Institute of Environmental Health, China Medical College, Taichung, Taiwan, Republic of China.
Chang
Pao-Wen
PW
Wu
Chin-Ching
CC
Kuo
Hsien-Wen
HW
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D016022:Case-Control Studies; D004247:DNA / Q000097:blood; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007854:Lead / Q000097:blood*; D007855:Lead Poisoning / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000235:genetics*; D016273:Occupational Exposure / Q000009:adverse effects*; D011524:Protoporphyrins / Q000097:blood; D012854:Sister Chromatid Exchange / Q000235:genetics*; D011795:Surveys and Questionnaires
D001798:Blood Proteins; D005819:Genetic Markers; D011524:Protoporphyrins; D007854:Lead; D004247:DNA