[Effect of CrCl3 on the in vitro production of flavonoids in a culture of Ononis arvensis L].
The paper investigated the effect of the abiotic elicitor chromium trichloride on the production of flavonoids in the callus and suspension cultures of Ononis arvensis L. after 12; 24; 48; 72, and 168-hour administration. The tested elicitor positively influenced the formation of flavonoids. A statistically significant increase in the flavonoid production in the callus culture was recorded with the use of a chromium trichloride solution in the concentration c1 (6.32 x 10(-4) mol/l) after 24; 48; 72, and 168 hours, in the concentration c2 (6.32 x 10(-6) mol/l) after 24; 48, and 72 hours, and in the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24, and 48 hours. In the suspension culture, the flavonoid production was significantly increased by the concentration c1 (6.32 x 10(-4) mol/l) after 12 and 72 hours, the concentration c2 (6.32 x 10(-6) mol/l) after 12; 24; 48, and 72 hours, and the concentration c3 (6.32 x 10(-8) mol/l) after 12; 24; 48, and 72 hours. The maximal increase in the flavonoid production by 98% took place in the callus culture after elicitation with a chromium trichloride in the concentration c3 (6.32 x 10(-8) mol/l) after 48 hours of administration, and in the suspension culture after elicitation with CrCl3 in the concentration c2 (6.32 x 10(-6) mol/l) after 12 hours by 100%.
Tůmová
L
L
Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové. tumova@faf.cuni.cz
Blazková
R
R
D002712:Chlorides / Q000494:pharmacology*; D017608:Chromium Compounds / Q000494:pharmacology*; D046508:Culture Techniques; D005419:Flavonoids / Q000096:biosynthesis*; D018547:Plant Stems / Q000378:metabolism*
D002712:Chlorides; D017608:Chromium Compounds; D005419:Flavonoids; C022990:chromic chloride