ARHI/NOEY2 inactivation may be important in breast tumor pathogenesis.
Allelic loss frequently occurs on the short arm of chromosome 1 in human breast carcinoma, suggesting that the ARHI/NOEY2 gene, an imprinted putative tumor suppressor gene, is involved in the pathogenesis of the tumor entity. To clarify the clinical importance of ARHI/NOEY2 mRNA in breast cancer, we studied whether ARHI/NOEY2 inactivation might contribute to tumors arising in the breast. An ARHI/NOEY2 message was detected by real-time PCR analysis in all noncancerous breast tissues, but was not detected in 2 of 26 breast cancer tissue samples. In 10 of 26 breast cancer tissue samples ARHI/NOEY2 mRNA was substantially reduced. ARHI/NOEY2 expression was lost or markedly reduced in 12 of 26 (46.15%) breast cancer tissue samples. In summary, we conclude that decreased ARHI/NOEY2 mRNA expression may play an important role in the pathogenesis of breast cancer.
Hisatomi
Hisashi
H
Center for Molecular Biology and Cytogenetics, SRL, Inc., Tokyo, Japan.
Nagao
Kumi
K
Wakita
Kazuyuki
K
Kohno
Norio
N
D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D020868:Gene Silencing; D016147:Genes, Tumor Suppressor; D018392:Genomic Imprinting; D006131:Growth Inhibitors / Q000378:metabolism*; D006801:Humans; D007564:Japan; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA; D012334:RNA, Neoplasm / Q000032:analysis; D020741:rho GTP-Binding Proteins
C117386:DIRAS3 protein, human; D006131:Growth Inhibitors; C034477:RNA primers; D012334:RNA, Neoplasm; D012313:RNA; D020741:rho GTP-Binding Proteins