Hepatitis C virus-like particle morphogenesis.
Although much is known about the hepatitis C virus (HCV) genome, first cloned in 1989, little is known about HCV structure and assembly due to the lack of an efficient in vitro culture system for HCV. Using a recombinant Semliki forest virus replicon expressing genes encoding HCV structural proteins, we observed for the first time the assembly of these proteins into HCV-like particles in mammalian cells. This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions.
Blanchard
Emmanuelle
E
Laboratoire de Virologie, E3M-EA3250, IFR 82, Faculté de Médecine et Centre Hospitalier Universitaire, Tours, France.
Brand
Denys
D
Trassard
Sylvie
S
Goudeau
Alain
A
Roingeard
Philippe
P
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D016174:Hepacivirus / Q000254:growth & development* / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D009024:Morphogenesis; D012093:Replicon; D012672:Semliki forest virus / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000502:physiology*; D019065:Virus Assembly
D015678:Viral Structural Proteins