[Effects of repeated high +Gz exposure on several enzyme activities in cardiomyocytes in rats and some protective measures].
Objective. To determine the changes of several myocardial enzymes in rats after repeated high +Gz exposure and the protective effects of preconditioning of low-G exposure and tea polyphenols (TP). Method. Thirty-two male Wistar rats were randomly divided into 4 groups (n = 8 each): control group (group A), +10 Gz group (group B), low-G preconditioning group (group C) and TP protection group (group D). Group B, C and D were exposed to repeated +10 Gz stress (each for 30s, 5 times/d with +1 Gz 1 min intervals, 3 d/wk, 3 weeks in total), but group A was only submitted to +1 Gz for 5 min. Group C was exposed to +2 Gz for 5 min about 1 h prior to +10 Gz stress. Additionally, TP (200 mg/kg) was given orally to group D about 1 h prior to the +Gz experiment, while distilled water was given to groups A, B and C instead. On the next day after the last centrifuge run, the hearts were taken out immediately for making frozen tissue sections. Enzyme histochemical staining and image analysis were carried out for acid phosphatase (ACP), succinate dehydrogenase (SDH), cytochromeoxidase (Cyt aa3) and adenosine triphosphatase (ATPase). Result. As compared with the control, the activities of ACP and SDH in +10 Gz stressed rats decreased significantly (P<0.05), and there was a declining trend for Cyt aa3. But, low-G preconditioning and TP had protective effects on +10 Gz stress-induced reduction of these enzymatic activities (P<0.05). Conclusion. The results showed that repeated high +Gz exposure could bring about decreases of activities in ACP (the marker enzyme of lysosome) and SDH (the marker enzyme of mitochondrial endomembrane), which indicated a reduction of oxidative metabolism in myocardial tissue; but preconditioning with low-G and natural antioxidant TP had protective effects.
Zhang
Z
Z
Institute of Aviation Medicine, Beijing, China.
Zhan
H
H
Lu
J Y
JY
Xin
Y M
YM
Li
T
T
Zhang
Q J
QJ
D000054:Acceleration; D000135:Acid Phosphatase / Q000378:metabolism*; D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005419:Flavonoids; D006321:Heart / Q000187:drug effects; D018471:Hypergravity; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013662:Tea
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases