An origin of DNA replication from streptomycete phage phi U1.
A DNA fragment from phage phi U1 containing an origin of DNA replication was identified. This fragment, designated ori, was able to support the maintenance in Streptomyces lividans of a plasmid lacking a functional Gram-positive ori. The sequence of the minimal ori fragment was determined and analyzed. The minimal fragment conferring replication origin function contained a number of direct and inverted repeats. The absence of an open reading frame in this ori fragment indicates that host factors alone were sufficient to initiate replication at ori.
Farkasovská
J
J
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
Godány
A
A
Bukovská
G
G
Timko
J
J
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000502:physiology*; D008969:Molecular Sequence Data; D018741:Replication Origin; D013302:Streptomyces / Q000821:virology*; D014779:Virus Replication
D004279:DNA, Viral