title: Chemokine production by rat macrophages stimulated with streptolysin O from Streptococcus pyogenes.
abstract: The contribution of streptolysin O (SLO) from Streptococcus pyogenes to neutrophil infiltration in inflammatory lesions was determined by production of cytokine-induced neutrophil chemoattractant (CINC)-1, -2 and -3, and macrophage inflammatory protein (MIP)-1alpha by rat macrophages stimulated with SLO in culture. Active SLO induced the production of CINCs and MIP-1alpha in dose- and time-dependent manners. These inductions were ascertained by chemokine mRNA expression in macrophages. Streptolysin S was without effect. The SLO-cholesterol complex induced the chemokine production in proportion to the residual hemolytic activity of the complex. In addition, the effects of SLO on the chemokine production were confirmed by the injection of active SLO into the preformed air pouch on the back of rats. The infiltration of neutrophils into the pouch fluid (exudate) increased steadily with a lag phase of about 2 hr. The major chemokine found in exudates was MIP-1alpha but not CINCs. In this study, it became clear that active SLO, but not the inactive one, contributed to the production of MIP-1alpha and CINCs in the conditioned medium and in exudates.
authors.affiliation: Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan.
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Mitsui
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takano
authors.forename: Sumie
authors.initials: S
authors.lastname: Nakatani
authors.forename: Hidemi
authors.initials: H
authors.lastname: Nambu
authors.forename: Futoshi
authors.initials: F
authors.lastname: Shibata
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002478:Cells, Cultured; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D018925:Chemokines / Q000096:biosynthesis*; D019743:Chemokines, CXC / Q000096:biosynthesis*; D002630:Chemotactic Factors / Q000096:biosynthesis*; D003470:Culture Media; D006461:Hemolysis; D007249:Inflammation / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis*; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis*; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000378:metabolism; D008297:Male; D020556:Neutrophil Infiltration; D051381:Rats; D017208:Rats, Wistar; D013297:Streptococcus pyogenes / Q000276:immunology* / Q000472:pathogenicity; D013301:Streptolysins / Q000276:immunology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D003470:Culture Media; C513297:Cxcl1 protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D019402:Macrophage Inflammatory Proteins; D013301:Streptolysins; C025964:streptolysin O