Interferon-gamma inhibits growth and migration of A172 human glioblastoma cells.
Malignant gliomas are highly proliferative and invasive tumors with poor prognosis. We investigated the influence of Interferon-gamma (IFN-gamma) on the human malignant glioma cell line A172, measuring cell viability (MTT-test), proliferation (3H-thymidine-uptake), cell death (FACS) adhesion to hyaluronic acid (HA, adhesion-assay) and migration (Boyden-chamber). IFN-gamma significantly decreased cell viability and proliferation. Measured by FACS, an up-regulation of CD95 expression has been shown in combination with an increased rate of cell death, first seen after 96 hours IFN-gamma treatment. Adhesion to HA was decreased after pre-treatment with IFN-gamma. This was not mediated by down-regulation of the main HA-receptor CD44, since IFN-gamma did not change CD44 expression. IFN-gamma-treated cells showed a significantly diminished migration rate through a native or HA-coated 8-microm polycarbonate membrane. To summarise, IFN-gamma influences both the main characteristics of malignancy: it decreases cell proliferation and induces cell death, further it diminishes migration of A172 human glioblastoma cells.
Knüpfer
M M
MM
Children's Hospital, Department of Neonatology, University of Leipzig, Germany. knuem@medizin.uni-leipzig.de
Knüpfer
H
H
Jendrossek
V
V
Van Gool
S
S
Wolff
J E
JE
Keller
E
E
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000096:biosynthesis
D000970:Antineoplastic Agents; D006131:Growth Inhibitors; D018960:Hyaluronan Receptors; D011994:Recombinant Proteins; D019014:fas Receptor; D007371:Interferon-gamma; D006820:Hyaluronic Acid