title: Calcium induced the damage of myocardial mitochondrial respiratory function in the early stage after severe burns.
abstract: OBJECTIVE
  To explore the role of Ca(2+) in the damage to myocardial mitochondrial respiratory function in the early stage after severe burns.
  
  
  METHODS
  An experimental model of 30%TBSA full-thickness skin scalding was reproduced in rats. Myocardial mitochondria were isolated from control and burned rats in the 1st, 3rd, 6th, 12th and 24th hour post-burn. The mitochondrial respiratory function, contents of mitochondrial calcium ([Ca(2+)](m)), activities of mtPLA(2), mtNOS, F(0)F(1)-ATPase and cytochrome c oxidase were determined.
  
  
  RESULTS
  (1) At the 1st hour post-burn, [Ca(2+)](m) was increased significantly and the myocardial mitochondrial respiratory function was significantly reinforced. At the same time, mitochondrial respiratory control rate (RCR) was elevated and positively correlated with [Ca(2+)](m) (r=0.8415, P<0.01). At the 3rd, 6th, 12th and 24th hour post-burn, [Ca(2+)](m) increased further to a higher level, however, the mitochondrial respiratory function was decreased from the peak value at 6h, and RCR was negatively correlated with [Ca(2+)](m). (2) The activities of mtNOS and mtPLA(2) were higher significantly at the 3rd, 6th, 12th and 24th hour post-burn than that of the control. After severe burns, mtNOS and mtPLA(2) activities were both positively correlated with [Ca(2+)](m) (r=0.8945, P<0.05; r=0.9271, P<0.01, respectively). (3) The F(0)F(1)-ATPase synthetic activity increased at the 1st hour post-burn, but it decreased to 51.4, 44.9, 77.6 and 87.4% of that of the control at the 3rd, 6th, 12th and 24th hour post-burn respectively. The F(0)F(1)-ATPase hydrolytic activity decreased at the 1st hour post-burn and increased at the 3rd, however, it decreased again at the 6th, 12th and 24th hour post-burn. The activity of cytochrome c oxidase at the 3rd, 6th, 12th and 24th hour was low compared to the control.
  
  
  CONCLUSIONS
  The changes of [Ca(2+)](m) were involved in damage to or regulation of mitochondrial respiratory function after severe burns. Appropriate increase of [Ca(2+)](m) reinforced the mitochondrial respiration at 1st hour after of burn injury, but Ca(2+) severe overload impairing F(0)F(1)-ATPase and cytochrome c oxidase directly, or, indirectly by activation of mtPLA(2) and mtNOS, might play an important role in damage to myocardial mitochondrial respiratory function at later stages after severe burns.
authors.affiliation: Institute of Burns Research, Southwest Hospital, Third Military Medical University, Chongqing, PR China. lwy@mail.tmmu.com.cn
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Liang
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Tang
authors.forename: Zong-Cheng
authors.initials: ZC
authors.lastname: Yang
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Huang
mesh_terms: D000818:Animals; D002056:Burns / Q000503:physiopathology*; D002118:Calcium / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009569:Nitric Oxide; D003576:Electron Transport Complex IV; D002118:Calcium