Speciation of seleno compounds in yeast aqueous extracts by three-dimensional liquid chromatography with inductively coupled plasma mass spectrometric and electrospray mass spectrometric detection.
A three-dimensional liquid chromatographic purification protocol based on sequential size-exclusion, anion-exchange and cation-exchange separation mechanisms was developed for the mapping of seleno compounds in aqueous yeast extracts. The method allowed the demonstration of the presence of more than 30 different seleno compounds. Semi-preparative size-exclusion and anion-exchange chromatography were optimized for maximum resolution using electrospray-compatible buffers in order to purify the compounds for mass spectrometric analysis. Molecular masses were attributed to many of the compounds on the basis of the selenium isotopic pattern in the electrospray mass spectra and of the collision-induced fragmentation patterns. Limitations preventing the ultimate identification of the selenium species detected are discussed.
McSheehy
Shona
S
Group of Bio-inorganic Analytical Chemistry, CNRS UMR 5034, Université de Pau et des Pays de l'Adour, France.
Pannier
Florence
F
Szpunar
Joanna
J
Potin-Gautier
Martine
M
Lobinski
Ryszard
R
D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D018036:Selenium Compounds / Q000032:analysis* / Q000737:chemistry
D018036:Selenium Compounds