title: [The polymorphism of four Y-STR loci and its application in forensic medicine].
abstract: We have obtained the alleles and haplotype distribution of 100 unrelated males from the Chinese Han and Zhuang populations at the four Y chromosome-specific loci A10, C4, A7.1, A7.2 by fluorescent primers and with 377 DNA sequencer. We observed 7, 6, 6, 6 alleles in loci A10, C4, A7.1, A7.2 respectively, the gene diversity (GD) values are 0.7776/0.629, 0.773/0.732, 0.5978/0.7272, 0.6664/0.6458 (Zhuang/Han). 114 haplotypes were found in 200 males, haplotype diversity (HD) values are 0.9786, 0.9772 (Zhuang/Han). We have confirmed the core repeats and its repeating number of these alleles by sequencing. A tetraplex PCR system consisting of the A10, C4, A7.1 and A7.2 loci was set up and it is of good specificity. We investigated the validation at these four Y-STRs loci in forensic application such as the male specificity, genetic stability and the sensitivity. In conclusion, the four Y-STRs loci are very suitable to forensic medicine analysis and paternity test.
authors.affiliation: Institute of Forensic Science, Beijing 100038, China.
authors.forename: Xiu-Fen
authors.initials: XF
authors.lastname: Zheng
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Cheng
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
authors.forename: Shao-Jian
authors.initials: SJ
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D002681:China / Q000208:ethnology; D005554:Forensic Medicine / Q000379:methods*; D005787:Gene Frequency; D014644:Genetic Variation; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D010334:Paternity; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012680:Sensitivity and Specificity; D014998:Y Chromosome