Molecular aptamer beacons for real-time protein recognition.
One of the most pressing problems facing those attempting to understand the regulation of gene expression and translation is the necessity to monitor protein production in a variety of metabolic states. Thus far, there is no easy solution that will either identify or quantitate proteins in real time. Here we introduce a novel protein probe, molecular aptamer beacon (MAB), for real time protein recognition and quantitative analysis. The MAB combines the signal transduction mechanism of molecular beacons and the molecular recognition specificity of aptamers. An MAB based on a thrombin-binding aptamer was prepared as a model to demonstrate the feasibility. Significant fluorescent signal change was observed when MAB was bound to thrombin, which is attributed to a significant conformational change in MAB from a loose random coil to a compact unimolecular quadruplex. The MAB recognizes its target protein with high specificity and high sensitivity (112 picomolar thrombin concentration) in homogeneous solutions. Ratiometric imaging has been conducted with MAB labeled with two fluorophores, which makes it feasible for protein quantitation in living specimen. The unique properties of the MAB will enable the development of a class of protein probes for real time protein tracing in living specimen and for efficient biomedical diagnosis in homogeneous solutions.
Li
Jianwei J
JJ
Department of Chemistry, McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA.
Fang
Xiaohong
X
Tan
Weihong
W
D001483:Base Sequence; D005240:Feasibility Studies; D005456:Fluorescent Dyes / Q000737:chemistry; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013917:Thrombin / Q000378:metabolism
D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D011506:Proteins; D013917:Thrombin