Growth factor-binding proteases in the murine submaxillary gland: isolation of a cDNA clone.
The submaxillary gland of the adult male mouse contains a number of serine proteases, several of which are involved in the proteolytic processing of precursors to growth factors and other biologically active polypeptides. Here we report the isolation and identification of a cDNA clone corresponding to one of the proteases, the type B of the epidermal growth factor-binding protein. A pronounced sequence homology was found between the predicted activation peptide of this protease and the NH2-terminal extension of the nerve growth factor alpha subunit, suggesting that the latter protein has an uncleaved activation peptide attached to its NH2 terminus.
Ronne
H
H
Department of Cell Research, University of Uppsala, Sweden.
Lundgren
S
S
Severinsson
L
L
Rask
L
L
Peterson
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D066298:In Vitro Techniques; D007610:Kallikreins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000235:genetics; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*; D020840:Tissue Kallikreins
D018076:DNA, Complementary; D006133:Growth Substances; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D020932:Nerve Growth Factor; D010450:Endopeptidases; D007610:Kallikreins; C489120:Klk13 protein, mouse; D012697:Serine Endopeptidases; D020840:Tissue Kallikreins; C489121:Klk1b22 protein, mouse; C489124:Klk26 protein, mouse; C489119:Klk9 protein, mouse; C053223:epidermal growth factor binding protein, mouse