Comet assay coupled to repair enzymes for the detection of oxidative damage to DNA induced by low doses of gamma-radiation: use of YOYO-1, low-background slides, and optimized electrophoresis conditions.
Sauvaigo
Sylvie
S
Laboratoire des Lésions des Acides Nucléiques, Département de Recherche Fondamentale sur la Matière Condensée, Service de Chimie Inorganique et Biologique-CNRS-UMR 5046, CEA Grenoble, F-38054 Grenoble Cedex 9, France.
Petec-Calin
Crina
C
Caillat
Sylvain
S
Odin
Francette
F
Cadet
Jean
J
D001583:Benzoxazoles / Q000737:chemistry*; D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects; D004996:Ethidium / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D005720:Gamma Rays; D006801:Humans; D008264:Macrophages; D018384:Oxidative Stress; D011806:Quinolinium Compounds / Q000737:chemistry*
D001583:Benzoxazoles; D005456:Fluorescent Dyes; D011806:Quinolinium Compounds; C075296:1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium; D004247:DNA; D004996:Ethidium