title: Hange-shashin-to raises levels of somatostatin, motilin, and gastrin in the plasma of healthy subjects.
abstract: Hange-shashin-to has been used for chronic hypofunction of the gastrointestinal tract and to improve functional abnormalities of the upper and lower gastrointestinal system. To determine whether the pharmacological effects of Hange-shashin-to are due to gut-regulatory peptide levels, we developed a sensitive and specific double-antibody enzyme immunoassay (EIA) for detecting motilin and also examined the levels of somatostatin-, motilin-, gastrin-, and vasoactive intestinal peptide (VIP)-immunoreactive substances (IS) in plasma from healthy subjects. We developed a sensitive (3.5 pg, 1.4 pg/well) and specific (carboxy-terminal region) EIA for motilin. A single oral administration of Hange-shashin-to 6.0 g caused significant increases somatostatin-IS (20-60 min), motilin-IS (40 min), and gastrin-IS (40-90 min) levels in plasma compared with levels in a placebo group. Hange-shashin-to had no significant effect on VIP-IS levels after single administration. These changes in hormone levels (somatostatin, motilin, and gastrin) might relate to normalization of the upper and lower gastrointestinal system by Hange-shashin-to.
authors.affiliation: Department of Clinical Pharmacy, Oita Medical University, Hasama-machi, Japan. naitou@oita-med.ac.jp
authors.forename: Takafumi
authors.initials: T
authors.lastname: Naito
authors.forename: Hiroki
authors.initials: H
authors.lastname: Itoh
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Yasunaga
authors.forename: Masaharu
authors.initials: M
authors.lastname: Takeyama
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005755:Gastrins / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D009037:Motilin / Q000097:blood*; D012016:Reference Values; D012680:Sensitivity and Specificity; D013004:Somatostatin / Q000097:blood*
chemical_list: D004365:Drugs, Chinese Herbal; D005755:Gastrins; C088236:hange-shashinto; D013004:Somatostatin; D009037:Motilin