Anti-HIV activity of a glycoprotein from first trimester placental tissue.
Human Immunodeficiency Virus-1 (HIV-1) transmission from a mother to the offspring during pregnancy is highly complex and a variable process in part due to the presence of several anti-HIV factors. In this study, we report an early pregnancy-associated protein (early pregnancy associated protein-1, Epap-1) with an apparent molecular weight of 90 kDa in placental tissue of pregnant women during the first trimester. Epap-1 was isolated and purified using Sambucus nigra agarose lectin affinity chromatography. Epap-1 strongly inhibits HIV-1(MN), HIV-1(91US056), HIV-1(VB-7) replication in vitro while it exhibits low activity with HIV-1(VB-66) strain. The molecular analysis of action of Epap-1 shows that it affects HIV-CD4 binding through inhibition of CD4-gp120 interaction.
Kondapi
Anand K
AK
Department of Biochemistry, School of Life Sciences, University of Hyderabad, India. akksl@uohyd.ernet.in
Hafiz
M A
MA
Sivaram
T
T
D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015704:CD4 Antigens / Q000378:metabolism; D002460:Cell Line; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011261:Pregnancy Trimester, First
D019380:Anti-HIV Agents; D015704:CD4 Antigens; D006023:Glycoproteins; D015699:HIV Envelope Protein gp120; D011257:Pregnancy Proteins; C455642:early pregnancy-associated protein 90 kDa, human