title: Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida.
abstract: An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ExoT exotoxins of Pseudomonas aeruginosa and to the YopE cytotoxin of different Yersinia species. The aexT gene was detected in all of the 12 A. salmonicida subsp. salmonicida strains tested but was absent from all other Aeromonas species. Recombinant AexT produced in Escherichia coli possesses enzymatic ADP-ribosyltransferase activity. Monospecific polyclonal antibodies directed against purified recombinant AexT detected the toxin produced by A. salmonicida subsp. salmonicida and cross-reacted with ExoS and ExoT of P. aeruginosa. AexT toxin could be detected in a wild type (wt) strain of A. salmonicida subsp. salmonicida freshly isolated from a fish with furunculosis; however, its expression required contact with RTG-2 rainbow trout gonad cells. Under these conditions, the AexT protein was found to be intracellular or tightly cell associated. No AexT was found when A. salmonicida subsp. salmonicida was incubated in cell culture medium in the absence of RTG-2 cells. Upon infection with wt A. salmonicida subsp. salmonicida, the fish gonad RTG-2 cells rapidly underwent significant morphological changes. These changes were demonstrated to constitute cell rounding, which accompanied induction of production of AexT and which led to cell lysis after extended incubation. An aexT mutant which was constructed from the wt strain with an insertionally inactivated aexT gene by allelic exchange had no toxic effect on RTG-2 cells and was devoid of AexT production. Hence AexT is directly involved in the toxicity of A. salmonicida subsp. salmonicida for RTG-2 fish cells.
authors.affiliation: Institute for Veterinary Bacteriology, Center for Fish and Wildlife Health, University of Bern, CH-3012 Bern, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Braun
authors.forename: Katja
authors.initials: K
authors.lastname: Stuber
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schlatter
authors.forename: Thomas
authors.initials: T
authors.lastname: Wahli
authors.forename: Peter
authors.initials: P
authors.lastname: Kuhnert
authors.forename: Joachim
authors.initials: J
authors.lastname: Frey
mesh_terms: D036002:ADP Ribose Transferases; D000333:Aeromonas / Q000201:enzymology*; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000502:physiology; D011065:Poly(ADP-ribose) Polymerases / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001427:Bacterial Toxins; D011994:Recombinant Proteins; D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; C444820:exoenzyme S