Method for the extraction of the volatile compound salicylic acid from tobacco leaf material.
Salicylic acid (SA) is a signalling compound in plants which is able to induce systemic acquired resistance. In the analysis of SA in plant tissues, the extraction recovery is often very low and variable. This is mainly caused by sublimation of SA, especially during evaporation of organic solvents. Techniques have been designed in order to overcome this problem. In the first part of the extraction procedure, sublimation of SA was prevented by addition of 0.2 M sodium hydroxide. At a later stage of the extraction procedure, sublimation of SA during solvent evaporation was controlled by the addition of a small amount of HPLC eluent. In this way, recoveries in the range of 71-91% for free SA and 65-79% for acid-hydrolysed SA were obtained. Recoveries could be further optimised by the use of an internal standard to correct for volume changes after the addition of the HPLC eluent.
Verberne
Marianne C
MC
Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research (LACDR), Leiden University, Leiden, The Netherlands.
Brouwer
Nynke
N
Delbianco
Federica
F
Linthorst
Huub J M
HJ
Bol
John F
JF
Verpoorte
Robert
R
D000085:Acetates; D002853:Chromatography, Liquid / Q000379:methods; D003510:Cyclohexanes; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification; D000432:Methanol; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D012459:Salicylates / Q000737:chemistry / Q000302:isolation & purification; D020156:Salicylic Acid / Q000737:chemistry / Q000302:isolation & purification*; D012996:Solutions; D014026:Nicotiana / Q000737:chemistry*; D014835:Volatilization
D000085:Acetates; D003510:Cyclohexanes; D005960:Glucosides; D012459:Salicylates; D012996:Solutions; C084746:salicylic acid glucoside; C007650:ethyl acetate; D020156:Salicylic Acid; D000432:Methanol