title: Homotypic cell-cell contacts stimulate the motile activity of rat prostate cancer cells.
abstract: OBJECTIVE
  To characterize the effect of homotypic cell-to-cell collisions upon the motile activities of two rat prostatic cancer cell lines of markedly different metastatic potential.
  
  
  METHODS
  The movements of strongly and weakly metastatic MAT-LyLu and AT-2 cells, respectively, were recorded under an inverted microscope at 37 degrees C. The motile activities of the cells at various cell densities were characterized quantitatively by computer-aided tracking methods and image analysis. The following variables were assessed: speed of movement, final displacement, coefficient of movement efficiency, diffusion constant and positive flow.
  
  
  RESULTS
  MAT-LyLu and AT-2 cells showed only limited motile activity in sparse cultures where there was little contact amongst the cells. However, under these and all other subsequent conditions tested, the motile activity of the MAT-LyLu cells was higher than the AT-2 cells. As the density of the cultured cells was increased (leading to more cell-to-cell contacts) there was a significant increase in motility. This effect was more pronounced for the AT-2 than for the MAT-LyLu cells, resulting in visible acceleration of movement by direct physical contact among the colliding cells. The motile activities of the tumour cells was only slightly affected by conditioned media.
  
  
  CONCLUSIONS
  Homotypic collisions between migrating prostatic cancer cells can strongly stimulate their motility. The effect of increased contact is greater on the weakly metastatic cells, such that at high cell density, the difference in the motilities of weak and strong metastatic cells is greatly reduced.
authors.affiliation: Department of Cell Biology, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland. Zibi@mol.uj.edu.pl
authors.forename: Z
authors.initials: Z
authors.lastname: Madeja
authors.forename: K
authors.initials: K
authors.lastname: Miekus
authors.forename: J
authors.initials: J
authors.lastname: Sroka
authors.forename: M B
authors.initials: MB
authors.lastname: Djamgoz
authors.forename: W
authors.initials: W
authors.lastname: Korohoda
mesh_terms: D000818:Animals; D002450:Cell Communication; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology*; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured