title: Membrane topology of the Streptococcus pneumoniae FtsW division protein.
abstract: The topology of FtsW from Streptococcus pneumoniae, an essential membrane protein involved in bacterial cell division, was predicted by computational methods and probed by the alkaline phosphatase fusion and cysteine accessibility techniques. Consistent results were obtained for the seven N-terminal membrane-spanning segments. However, the results from alkaline phosphatase fusions did not confirm the hydropathy analysis of the C-terminal part of FtsW, whereas the accessibility of introduced cysteine residues was in agreement with the theoretical prediction. Based on the combined results, we propose the first topological model of FtsW, featuring 10 membrane-spanning segments, a large extracytoplasmic loop, and both N and C termini located in the cytoplasm.
authors.affiliation: Institut de Biologie Structurale Jean-Pierre Ebel (CEA/CNRS/UJF), Laboratoire d'Ingénierie des Macromolécules, 38027 Grenoble Cedex 1, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gérard
authors.forename: Thierry
authors.initials: T
authors.lastname: Vernet
authors.forename: André
authors.initials: A
authors.lastname: Zapun
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003545:Cysteine / Q000235:genetics; D007118:Immunoassay; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013296:Streptococcus pneumoniae / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C061684:FtsW protein, Bacteria; D000469:Alkaline Phosphatase; D003545:Cysteine