title: Chlamydia pneumoniae IgA- and IgG antibodies in young survivors of myocardial infarction. A comparison of antibody detection by a microimmunofluorescence test and an enzyme immunoassay.
abstract: OBJECTIVE
  Chronic Chlamydia pneumoniae infection is considered as a cardiovascular risk factor and antibodies are commonly analysed by the subjective microimmunofluorescence (MIF) test. We wanted to investigate the C. pneumoniae IgA- and IgG seroprevalence in young survivors of myocardial infarction and matched controls, and to compare the agreement of detecting antibodies between a MIF test and an enzyme immunoassay (EIA).
  
  
  METHODS
  A total of 61 patients hospitalized as a result of myocardial infarction, 51 patients hospitalized with chest pain and negative exercise-ECG and 61 age and sex matched controls (mean age 53.3 years, range 40-60 years) were included in this case-control study. Serological comparisons were expressed as sensitivity, specificity and interrater agreement (K or Kw) of the EIA test related to the MIF test.
  
  
  RESULTS
  Presence of IgA (cut off = 16) antibodies was significantly higher in coronary heart patients compared with controls for both assays (P = 0.02 by the MIF and P = 0.05 by the EIA test). The presence of IgG (cut off = 32) antibodies was significantly higher amongst patients (P = 0.05) when analysed by the MIF-test, but not with the EIA-test (P = 0.16). The strength of agreement between the assays was good for both IgA- (Kw = 0.67) and IgG (Kw = 0.79) analyses. However, only 52.8% of the IgA samples classified as strong positive (cut-off = 32) by the MIF test were strong positive by the EIA test (K = 0.56). Only 73.2% of the negative IgG samples (<32) by the MIF-test turned out negative by the EIA-test (K = 0.73).
  
  
  CONCLUSIONS
  Dependent on assay and cut-off level, there is an increased C. pneumoniae IgA- and IgG seroprevalence in young survivors of myocardial infarction compared with controls. Despite the subjective interpretation of MIF-titres, the strength of agreement between the EIA and MIF tests was good for both antibody classes. However, misclassification of highly positive IgA samples and negative IgG samples by the MIF test may influence study conclusions. We conclude that the choice of serological method is of major importance when evaluating a possible relationship between C. pneumoniae and coronary heart disease.
authors.affiliation: Department of Microbiology, University Hospital of Tromsø, Norway. mlabdsh@rito.no
authors.forename: D S
authors.initials: DS
authors.lastname: Halvorsen
authors.forename: T
authors.initials: T
authors.lastname: Børvik
authors.forename: I
authors.initials: I
authors.lastname: Njølstad
authors.forename: T J
authors.initials: TJ
authors.lastname: Gutteberg
authors.forename: L H
authors.initials: LH
authors.lastname: Vorland
authors.forename: J B
authors.initials: JB
authors.lastname: Hansen
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000276:immunology* / Q000382:microbiology; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G