Targetted localisation and imaging of a murine lymphoma using 131I-labelled monoclonal antibody.
In vivo tumor targetting with radiolabelled monoclonal antibodies is a promising approach for the diagnosis and therapy of tumors. A specific monoclonal antibody (mAb), DLAB was generated to the Dalton's lymphoma associated antigen (DLAA) from Haemophilus paragallinarum-induced spontaneous fusion. In order to study the tumor localisation and biodistribution properties of the monoclonal antibody, scintigraphic studies were performed using the radiolabelled DLAB. 131-labelled DLAB was administered intravenously into Swiss mice bearing Dalton's lymphoma and external scintiscanning was performed at different time intervals. Clear tumor images were obtained which revealed selective and specific uptake of radiolabel and the results were compared with biodistribution data. The radioiodinated monoclonal antibody showed fast tumor uptake which increased significantly to 14.6% injected dose (ID)/g at 12 hr post-injection. Enhanced blood clearance of radioactivity resulted in higher tumor/blood ratio of 5.96 at 48 hr. 131I-labelled DLAB resulted in selective and enhanced uptake of the radioactivity by the tumor compared to the non-specific antibody and the results suggest the potential use of spontaneous fusion for producing specific monoclonal antibodies for tumor detection and therapy.
Subbiah
K
K
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. skrishnan@mail.com
Rayala
S K
SK
Ananthanarayanan
M
M
Thangarajan
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000276:immunology*; D004195:Disease Models, Animal; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008223:Lymphoma / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D016719:Radioimmunodetection / Q000379:methods; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007457:Iodine Radioisotopes