Altered kinetics of IL-1 alpha, IL-1 beta, and KGF-1 gene expression in early wounds of restrained mice.
Inflammatory processes that occur after injury contribute to wound closure. Previous studies showed that wounds of restraint-stressed (RST) mice had a reduced number of inflammatory cells and healed more slowly compared to controls. To investigate the molecular mechanisms between stress and wound healing, we studied cutaneous gene expression of IL-1 alpha, IL-1 beta, and KGF-1. Female SKH-1 mice were restrained for 3 days before and for 5 days following placement of cutaneous wounds. Wounds were subjected to competitive RT-PCR. At day 1, RST mice had significantly lower IL-1 beta and KGF-1 mRNA than controls. At day 5, RST mice had significantly higher IL-1 alpha and IL-1 beta mRNA than controls. Treatment of RST mice with the glucocorticoid receptor antagonist RU486 restored IL-1 beta mRNA expression at day 1. Our results suggest that stress induces alterations in the kinetics of cutaneous proinflammatory cytokine and growth factor gene expression which could impair the quality of healing tissues.
Mercado
Ana M
AM
Department of Oral Biology, College of Dentistry, The Ohio State University, Columbus, 43210, USA.
Padgett
David A
DA
Sheridan
John F
JF
Marucha
Phillip T
PT
D000818:Animals; D005260:Female; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000037:antagonists & inhibitors / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D007375:Interleukin-1 / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D015735:Mifepristone / Q000008:administration & dosage*; D012149:Restraint, Physical / Q000009:adverse effects*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013315:Stress, Psychological / Q000276:immunology*; D013997:Time Factors; D014945:Wound Healing / Q000235:genetics / Q000276:immunology*; D014947:Wounds and Injuries / Q000276:immunology
C496454:Fgf7 protein, mouse; D005938:Glucocorticoids; D007375:Interleukin-1; D051523:Fibroblast Growth Factor 7; D015735:Mifepristone; D005346:Fibroblast Growth Factors