Mutational analysis of the enterocin favorskii biosynthetic rearrangement.
[reaction: see text] A mutational analysis of the enterocin biosynthesis genes revealed that the putative oxygenase and the methyltransferase gene products EncM and EncK, respectively, jointly catalyze a biosynthetic Favorskii-like rearrangement. Inactivation of either gene terminated enterocin production and caused the accumulation of four nonrearranged, nonmethylated polyketides. The structure elucidation of the new wailupemycins E-G is reported.
Xiang
Longkuan
L
College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA.
Kalaitzis
John A
JA
Nilsen
George
G
Chen
Li
L
Moore
Bradley S
BS
D001952:Bridged-Ring Compounds / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D013302:Streptomyces / Q000737:chemistry
D001952:Bridged-Ring Compounds; C012306:enterocin