Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel.
Application of ultraviolet light irradiation to a photocrosslinkable chitosan aqueous solution resulted in an insoluble, flexible hydrogel like soft rubber within 60 seconds. In order to evaluate its accelerating effect on wound healing, full-thickness skin incisions were made on the backs of mice and subsequently a photocrosslinkable chitosan aqueous solution was added into the wound and irradiated with UV light for 90 seconds. Application of the chitosan hydrogel significantly induced wound contraction and accelerated wound closure and healing compared with the untreated controls. Histological examination also showed an advanced contraction rate on the first 2 days and tissue fill rate on days 2 to 4 in the chitosan hydrogel-treated wounds. Furthermore, in cell culture studies, chitosan hydrogel culture medium supplemented with 5% fetal-bovine serum was found to be chemoattractant for human dermal fibroblasts in an invasion chamber assay using filters coated with Matrigel and in a cell migration assay. Due to its ability to accelerate wound contraction and healing, chitosan hydrogel may become accepted as an occlusive dressing for wound management.
Ishihara
M
M
Research Institute, and Departments of Surgery II, Orthopedic Surgery, and Medical Engineering, National Defense Medical College, and NeTech Inc., Tokorozawa, Saitama, Japan. ishihara@res.ndmc.ac.jp
Ono
K
K
Sato
M
M
Nakanishi
K
K
Saito
Y
Y
Yura
H
H
Matsui
T
T
Hattori
H
H
Fujita
M
M
Kikuchi
M
M
Kurita
A
A
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001704:Biopolymers / Q000008:administration & dosage*; D002465:Cell Movement; D002478:Cells, Cultured; D002686:Chitin / Q000008:administration & dosage* / Q000031:analogs & derivatives; D048271:Chitosan; D003432:Cross-Linking Reagents; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D020100:Hydrogels; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000166:cytology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D001672:Biocompatible Materials; D001704:Biopolymers; D003432:Cross-Linking Reagents; D020100:Hydrogels; D002686:Chitin; D048271:Chitosan