Determination of difloxacin and sarafloxacin in chicken muscle using solid-phase extraction and capillary electrophoresis.
This paper describes a method for residue analysis of difloxacin and sarafloxacin in chicken muscle. Clean-up and preconcentration of the samples are effected by solid-phase extraction (C18) and the determination is carried out by capillary electrophoresis using a photodiode array detection system. The method was validated with satisfying results. The calibration graphs are linear for difloxacin and sarafloxacin from 50 to 300 microg/kg. The limit of detection obtained for difloxacin and sarafloxacin are 10 and 25 microg/kg, respectively, which allows the detection of positive muscle samples at the required maximum residue limits of European Union.
Barrón
D
D
Department of Analytical Chemistry, University of Barcelona, Spain.
Jiménez-Lozano
E
E
Bailac
S
S
Barbosa
J
J
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002645:Chickens; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D024841:Fluoroquinolones; D009132:Muscles / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D002939:Ciprofloxacin; C047225:difloxacin; C047224:sarafloxacin