Melatonin improves deferoxamine antioxidant activity in protecting against lipid peroxidation caused by hydrogen peroxide in rat brain homogenates.
Deferoxamine (DF) is an antioxidant molecule because of its ability to chelate iron. This study compared the ability of DF alone or in combination with melatonin, 5-methoxytryptophol or pinoline in preventing lipid peroxidation due to hydrogen peroxide (H(2)O(2)) in rat brain homogenates. Malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) in the homogenates were measured as indices of lipid peroxidation. Incubation of homogenates with DF reduced, in a dose-dependent manner, MDA+4-HDA formation due to H(2)O(2). When melatonin, 5-methoxytryptophol or pinoline were added to the incubation medium, the efficacy of DF in preventing lipid peroxidation was enhanced. These cooperative effects between DF, melatonin, and related pineal products may be important in protecting tissues from the oxidative stress due to iron overload.
Ortega-Gutiérrez
S
S
Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain.
García
J J
JJ
Martínez-Ballarín
E
E
Reiter
R J
RJ
Millán-Plano
S
S
Robinson
M
M
Acuña-Castroviejo
D
D
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000009:adverse effects* / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D007502:Iron Chelating Agents; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D003676:Deferoxamine; D008550:Melatonin