title: Use of ligase chain reaction for laboratory identification of Chlamydia trachomatis and Neisseria gonorrhoeae in adolescent women.
abstract: OBJECTIVE
  To compare the ligase chain reaction (LCR) with culture for the detection of Neisseria gonorrhoeae (GC) and with culture and direct fluorescent antibody (DFA) for identification of Chlamydia trachomatis (CT) in cervical specimens from adolescent women.
  
  
  METHODS
  A prospective study of test performance.
  
  
  METHODS
  Two urban, hospital-based adolescent clinics.
  
  
  METHODS
  Adolescent women aged 12-22 yr undergoing pelvic examination for routine sexually transmitted disease (STD) screening or symptoms suggestive of an STD.
  
  
  METHODS
  LCR results were considered to be true positives if confirmed by culture and/or DFA (CT only). Discrepant LCR results were confirmed by testing an alternative locus.
  
  
  RESULTS
  With 538 subjects, LCR for CT had a sensitivity of 98.4% (61/62) and specificity of 96.4% (459/476) prior to resolution and a sensitivity of 98.6% (70/71) and specificity of 99.6% (459/461) after resolution. With 1225 subjects, LCR for GC had a sensitivity of 90.0% (54/60) and specificity of 99.4% (1158/1165) prior to resolution and a sensitivity of 90.6% (58/64) and specificity of 100% (1158/1158) after resolution. CT culture alone identified 80% of the true positives and DFA alone only identified 72%. GC culture alone identified 94% of the true positives.
  
  
  CONCLUSIONS
  LCR is an extremely sensitive and specific rapid test, utilizing a single swab and convenient room-temperature storage and transport of specimens. LCR testing of cervical specimens for CT in adolescent women is a better test for detecting CT infection than culture or DFA. LCR testing for cervical GC infection may provide an advantage over culture in circumstances in which optimal transport conditions and viability of the organism cannot be assured.
authors.affiliation: Department of Pediatrics, Section of Adolescent Medicine, St. Christopher's Hospital for Children, MCP-Hahnemann University School of Medicine, Philadelphia, PA 19134, USA.
authors.forename: Paula K
authors.initials: PK
authors.lastname: Braverman
authors.forename: Donald F
authors.initials: DF
authors.lastname: Schwarz
authors.forename: Md
authors.initials: M
authors.lastname: Mph
authors.forename: Adamadia
authors.initials: A
authors.lastname: Deforest
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hodinka
authors.forename: Karin L
authors.initials: KL
authors.lastname: McGowan
authors.forename: Joel E
authors.initials: JE
authors.lastname: Mortensen
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial