Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes.
In this study, we have isolated a bovine homologue bgl-1 of lethal giant larvae (lgl) tumor suppressor oncogene from bovine brain by RT-PCR using primers designed based on the conserved sequences for lgl family members. The sequence analysis showed that the bgl-1 encodes a 1,036 amino acid polypeptide with the molecular weight of approximately 112 kDa containing a domain characteristic of WD-40 proteins. The amino acid sequence of bgl-1 showed a homology of 98.3 and 87.3% identity to that of mouse and human, respectively. Northern blot analysis showed that bgl-1 was highly expressed in brain, ovary and testis, with moderate expression in liver, uterus, lung and kidney. This suggests that the bgl-1 may play essential roles in each of these organs. The complementation analysis revealed that the bovine bgl-1 partially restored the Na+ tolerance in the absence of yeast lgl homologue, suggesting that bgl-1 is a bovine homologue of the lgl family.
Baek
Kwang-Hyun
KH
College of Medicine, Cell and Gene Therapy Research Institute, Pochon CHA University, CHA General Hospital, Seoul, Korea 135-081. baek@cha.ac.kr
Kim
Yong-Soo
YS
Jung
Sunki
S
Lee
Kwang Youl
KY
Choi
Hee-Kyung
HK
Kim
Kye-Seong
KS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000502:physiology; D002417:Cattle; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D016147:Genes, Tumor Suppressor; D005816:Genetic Complementation Test; D015801:Helminth Proteins / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins
D017931:DNA Primers; D029721:Drosophila Proteins; D015801:Helminth Proteins; D019476:Insect Proteins; D011506:Proteins; D025521:Tumor Suppressor Proteins; C400683:che-2 protein, C elegans; C081435:l(2)gl protein, Drosophila