HIV-1 envelope glycoprotein 120 increases intercellular adhesion molecule-1 expression by human endothelial cells.
Human immunodeficiency virus type 1 (HIV-1) infection is often associated with central nervous system damage and vascular complications. However, the mechanisms of this association are largely unknown. We examined the effect of HIV-1 envelope glycoprotein 120 (gp120) on cell adhesion molecule expression by endothelial cells. We found, for the first time, that both soluble and membrane-bound gp120 could significantly increase the expression of human endothelial intercellular adhesion molecule-1 (ICAM-1) at both mRNA and protein levels, but not vascular cell adhesion molecule-1 and E-selectin. The specificity of gp120-mediated response was demonstrated by blocking experiments using a specific monoclonal antibody against gp120, which successfully abolished the gp120-mediated increase of ICAM-1 expression. Furthermore, there was a significant increase of human monocytic cell line THP-1 adherence onto the gp120-treated endothelial monolayers. This increased cell adhesion was effectively blocked by either anti-gp120 or anti-ICAM antibodies. These findings suggest that HIV-1 gp120-mediated endothelial ICAM-1 expression could be one of the important mechanisms of HIV-1 pathogenesis.
Ren
Zeguang
Z
Department of Surgery, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Yao
Qizhi
Q
Chen
Changyi
C
D019138:Antibodies, Blocking / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D015497:HIV-1; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D015699:HIV Envelope Protein gp120; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1