Characterization of toxin from cheilanthes fern and its effect on lymphocyte proliferation and DNA fragmentation.
Thin layer chromatography of aqueous extract of whole Cheilanthesfarinosa fern indicated the presence of ptaquiloside or ptaquiloside like compound, coinciding Rf values with that of Pterosin B standard. HPLC analysis revealed the presence of 26.3 mg/kg ptaquiloside. In vitro studies of the aqueous extract on lymphocyte culture revealed a correlation between stimulative indices and concentration of aqueous extract. Stimulation in lymphocyte proliferation was in order of bracken > cheilanthes > ConA> ptaquiloside standard. On incubation of lymphocyte with aqueous extract of ferns, no DNA damage was observed in isolated DNA.
Kumar
K A
KA
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar.
Kataria
M
M
Somvanshi
R
R
Kumar
S
S
Saini
M
M
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D053938:DNA Fragmentation / Q000187:drug effects*; D029624:Ferns / Q000737:chemistry*; D005562:Formazans / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D010936:Plant Extracts / Q000633:toxicity*; D010947:Plants, Toxic / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000378:metabolism
D005562:Formazans; D010936:Plant Extracts; D013778:Tetrazolium Salts; C079782:MTT formazan