Functional expression of a locust tyramine receptor in murine erythroleukaemia cells.
The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (TyrLoc), an insect G protein-coupled receptor. Functional agonist-dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL-TyrLoc). Tyramine elicited a dose-dependent increase of cytosolic Ca2+-ions and an attenuation of forskolin-induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein-coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.
Poels
J
J
Laboratory for Developmental Physiology and Molecular Biology, Zoological Institute, KULeuven, Naamsestraat 59, B-3000 Leuven, Belgium.
Suner
M M
MM
Needham
M
M
Torfs
H
H
De Rijck
J
J
De Loof
A
A
Dunbar
S J
SJ
Vanden Broeck
J
J
D000818:Animals; D001483:Base Sequence; D002118:Calcium; D000242:Cyclic AMP; D018076:DNA, Complementary; D015870:Gene Expression; D006110:Grasshoppers / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008969:Molecular Sequence Data; D017982:Receptors, Biogenic Amine / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D017982:Receptors, Biogenic Amine; C066175:tyramine receptor; D000242:Cyclic AMP; D002118:Calcium