Studies on the permeability of calf thymus nuclei isolated in sucrose.
A study of the permeability of calf thymus nuclei isolated in sucrose was carried out with sucrose-14C, glycerol-14C, and carboxydextran-14C (molecular weight, 60,000-90,000). The results indicate that the nuclei are very permeable to both sucrose and glycerol but they exclude the carboxydextran. Results obtained with other low molecular weight non-electrolytes (malonamide-14C, erythritol-14C, D-arabinose-14C, and D-mannitol-14C) are in agreement with the view that the nuclei are freely permeable to these molecular species. A sucrose-impermeable space is also present in these preparations and it has been attributed to the presence of intact cells. The high permeability of nuclei to sucrose was confirmed with Ficoll-separated preparations. The possibility of the presence of a substantial particulate space that allows the penetration of dextran cannot be excluded by these experiments, and this space may correspond to damaged nuclei.
Kodama
R M
RM
Department of Physiology, University of Illinois, Medical Center, Chicago, Illinois, USA.
Tedeschi
H
H
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D003911:Dextrans / Q000494:pharmacology; D004573:Electrolytes / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D008854:Microscopy, Electron; D010539:Permeability; D013395:Sucrose / Q000494:pharmacology*; D013696:Temperature; D013950:Thymus Gland / Q000166:cytology*; D013997:Time Factors
D000925:Anticoagulants; D003911:Dextrans; D004573:Electrolytes; D013395:Sucrose; D005990:Glycerol