Simultaneous analysis of gamma-hydroxybutyric acid, gamma-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography.
A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of gamma-hydroxybutyric acid (GHB), gamma-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0mM sodium barbital and 150.0mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients (r(2)) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1mg/l, 0.34 and 0.25g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. (n = 9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions.
Dahlén
Johan
J
SKL-National Laboratory of Forensic Science, SE-581 94, Linköping, Sweden. drog@skl.police.se
Vriesman
Thyrsa
T
D015107:4-Butyrolactone / Q000032:analysis*; D002072:Butylene Glycols / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006885:Hydroxybutyrates / Q000032:analysis*; D012997:Solvents / Q000032:analysis*
D002072:Butylene Glycols; D006885:Hydroxybutyrates; D012997:Solvents; C111420:4-hydroxybutyric acid; C039681:1,4-butanediol; D015107:4-Butyrolactone