title: Transient receptor potential 1 regulates capacitative Ca(2+) entry and Ca(2+) release from endoplasmic reticulum in B lymphocytes.
abstract: Capacitative Ca(2+) entry (CCE) activated by release/depletion of Ca(2+) from internal stores represents a major Ca(2+) influx mechanism in lymphocytes and other nonexcitable cells. Despite the importance of CCE in antigen-mediated lymphocyte activation, molecular components constituting this mechanism remain elusive. Here we demonstrate that genetic disruption of transient receptor potential (TRP)1 significantly attenuates both Ca(2+) release-activated Ca(2+) currents and inositol 1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release from endoplasmic reticulum (ER) in DT40 B cells. As a consequence, B cell antigen receptor-mediated Ca(2+) oscillations and NF-AT activation are reduced in TRP1-deficient cells. Thus, our results suggest that CCE channels, whose formation involves TRP1 as an important component, modulate IP(3) receptor function, thereby enhancing functional coupling between the ER and plasma membrane in transduction of intracellular Ca(2+) signaling in B lymphocytes.
authors.affiliation: Center for Integrative Bioscience, Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji-cho, Okazaki 444-8585, Japan. moriy@nips.ac.jp
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Mori
authors.forename: Minoru
authors.initials: M
authors.lastname: Wakamori
authors.forename: Tomoya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Meredith
authors.initials: M
authors.lastname: Hermosura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hara
authors.forename: Motohiro
authors.initials: M
authors.lastname: Nishida
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hirose
authors.forename: Akiko
authors.initials: A
authors.lastname: Mizushima
authors.forename: Mari
authors.initials: M
authors.lastname: Kurosaki
authors.forename: Emiko
authors.initials: E
authors.lastname: Mori
authors.forename: Kumiko
authors.initials: K
authors.lastname: Gotoh
authors.forename: Takaharu
authors.initials: T
authors.lastname: Okada
authors.forename: Andrea
authors.initials: A
authors.lastname: Fleig
authors.forename: Reinhold
authors.initials: R
authors.lastname: Penner
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Iino
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kurosaki
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000235:genetics; D002460:Cell Line; D002645:Chickens; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D050052:TRPC Cation Channels
chemical_list: D015220:Calcium Channels; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D002118:Calcium