title: In vitro adhesive properties and virulence factors of Enterococcusfaecalis strains.
abstract: Twenty-nine Enterococcus faecalis isolates from patients with endocarditis or bacteremia or from stools of healthy volunteers were investigated for their ability to adhere to Int-407 and Girardi heart cell lines and for the presence of known enterococcal virulence factors. Eight strains (27.6%) adhered predominantly to Int-407 cells. The adherence of enterococci was enhanced by proteolytic digestion, suggesting that some cell binding components become surface-exposed after treatment with trypsin. The occurrence of known potential virulence factors of enterococci among these strains was determined and was as follows: enterococcal surface protein (72.4%), gelatinase (58.6%), aggregation substance (48.3%) and cytolysin (17.2%). Bacterial adherence was not significantly associated with any of these virulence factors.
authors.affiliation: Laboratoire de Bactériologie-Virologie, Université d'Auvergne, Faculté de Pharmacie, Clermont-Ferrand, France. Christine.ARCHIMBAUD@u-clermont1.fr
authors.forename: Christine
authors.initials: C
authors.lastname: Archimbaud
authors.forename: Nathan
authors.initials: N
authors.lastname: Shankar
authors.forename: Christiane
authors.initials: C
authors.lastname: Forestier
authors.forename: Arto
authors.initials: A
authors.lastname: Baghdayan
authors.forename: Michael S
authors.initials: MS
authors.lastname: Gilmore
authors.forename: Françoise
authors.initials: F
authors.lastname: Charbonné
authors.forename: Bernard
authors.initials: B
authors.lastname: Joly
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000472:pathogenicity* / Q000502:physiology; D005243:Feces / Q000382:microbiology*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D014357:Trypsin / Q000378:metabolism; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D014357:Trypsin