title: Analysis of octamer-binding transcription factors Oct2 and Oct1 and their coactivator BOB.1/OBF.1 in lymphomas.
abstract: Oct1 and Oct2 are transcription factors of the POU homeo-domain family that bind to the Ig gene octamer sites, regulating B-cell-specific genes. The function of these transcription factors is dependent on the activity of B-cell-restricted coactivators such as BOB.1/OBF.1. Independent studies of the expression of these proteins in non-Hodgkin's lymphoma have been restricted to single markers, and most lack data concerning immunohistochemical expression. Thus, we have investigated the expression of Oct1, Oct2, and BOB.1/OBF.1 in human reactive lymphoid tissue and in a series of 140 Hodgkin and non-Hodgkin's lymphomas. None of these proteins was found to be restricted to B cells, although only B cells expressed high levels of all three markers. Additionally, germinal center B cells showed stronger Oct2 and BOB.1/OBF.1 staining. Consequently, most B-cell lymphomas showed reactivity for all three antibodies. Oct2 expression was significantly higher in germinal center-derived lymphomas, although other B-cell lymphomas also displayed a high level of Oct2 expression. Although T-cell lymphomas and Hodgkin's lymphomas expressed some of these proteins, they commonly exhibited less reactivity than B-cell lymphomas. Despite not being entirely cell-specific, the strong nuclear expression of Oct2 and BOB.1/OBF.1 by germinal center- derived lymphomas makes these antibodies a potentially useful tool in lymphoma diagnosis.
authors.affiliation: Molecular Pathology Program, Centro Nacional de Investigaciones Oncológicas Carlos III, Madrid, Spain.
authors.forename: Ana-Isabel
authors.initials: AI
authors.lastname: Sáez
authors.forename: María-Jesús
authors.initials: MJ
authors.lastname: Artiga
authors.forename: Margarita
authors.initials: M
authors.lastname: Sánchez-Beato
authors.forename: Lydia
authors.initials: L
authors.lastname: Sánchez-Verde
authors.forename: Juan-Fernando
authors.initials: JF
authors.lastname: García
authors.forename: Francisca-Inmaculada
authors.initials: FI
authors.lastname: Camacho
authors.forename: Renato
authors.initials: R
authors.lastname: Franco
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Piris
mesh_terms: D018951:Antigens, CD20 / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D019084:Fluorescent Antibody Technique, Indirect; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008228:Lymphoma, Non-Hodgkin / Q000378:metabolism* / Q000473:pathology; D050811:Octamer Transcription Factor-1 / Q000378:metabolism*; D050813:Octamer Transcription Factor-2 / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D018951:Antigens, CD20; D050811:Octamer Transcription Factor-1; D050813:Octamer Transcription Factor-2; C091546:POU2AF1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors