Repression of the 14-3-3 gene affects the amino acid and mineral composition of potato tubers.
Recently, transgenic potato plants were created showing underexpression of the 20R isoform of the 14-3-3 protein. The transgenic plants grown in tissue culture showed a significant increase in nitrate reductase activity and a decrease in nitrate level. The transgenic line with the lowest 14-3-3 quantity was field-trialed (1997-2000) and analyzed. The reduction in the 14-3-3 protein level consistently resulted in a starch content increase and in an increase in the ratio of soluble sugars to starch in the tubers, although the latter was only barely visible. The determination of amino acid composition in the tubers showed a significant increase in methionine, proline, and arginine content and a slight but consistent increase in hydrophobic amino acid and lysine content in the cells of the transgenic potato plants. We also observed an increase in the crude protein content, from 19 to 22.1% of the control value in consecutive years. It is proposed that all of these changes might have resulted from the downregulation of nitrate reductase and sucrose phosphate synthase activities by 14-3-3, although other potential mechanisms cannot be excluded (e.g., an increase in enzyme protein level). 14-3-3-repressed transgenic plants showed a significant increase in calcium content in their tubers. It is thus proposed that a function of the isolated 14-3-3 isoform is in the control of amino acid synthesis and calcium metabolism. However, the mechanism of this control is as yet unknown.
Swiedrych
Anna
A
Institute of Biochemistry and Molecular Biology, University of Wrocław, Institute of Plant Genetics, Polish Academy of Science, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
Prescha
Anna
A
Matysiak-Kata
Iwona
I
Biernat
Jadwiga
J
Szopa
Jan
J
D048948:14-3-3 Proteins; D000596:Amino Acids / Q000032:analysis*; D001120:Arginine / Q000032:analysis; D046508:Culture Techniques; D015870:Gene Expression; D008715:Methionine / Q000032:analysis; D008903:Minerals / Q000032:analysis*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000032:analysis; D010641:Phenotype; D010940:Plant Proteins / Q000032:analysis; D030821:Plants, Genetically Modified; D011392:Proline / Q000032:analysis; D011198:Solanum tuberosum / Q000737:chemistry / Q000235:genetics*; D013213:Starch / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000172:deficiency / Q000235:genetics* / Q000502:physiology
D048948:14-3-3 Proteins; D000596:Amino Acids; D008903:Minerals; D009566:Nitrates; D010940:Plant Proteins; D013213:Starch; D001120:Arginine; D011392:Proline; D008715:Methionine; D014446:Tyrosine 3-Monooxygenase; D009565:Nitrate Reductases; D050901:Nitrate Reductase