Prevalence of putative periodontopathogens from periodontal patients and healthy subjects in Sao Paulo, SP, Brazil.
A. actinomycetemcomitans, B. forsythus, P. gingivalis, C. rectus, E. corrodens, P. intermedia, F. nucleatum, and T. denticola were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. PCR products from each species showed a specific band and could be used to identify periodontal organisms from clinical specimens. Identical negative or positive results between PCR and culture occurred in 66% (A. actinomycetemcomitans) to 93% (F. nucleatum) of the samples. PCR detection odds ratio values for A. actinomycetemcomitans, B. forsythus, C. rectus, E. corrodens, P. intermedia, and T. denticola were significantly associated with disease having a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70). Cultures showed that A. actinomycetemcomitans, B. forsythus and P. intermedia were associated with periodontitis, however, P. gingivalis, C. rectus, E. corrodens and F. nucleatum were not significantly associated with the disease.
Avila-Campos
Mario J
MJ
Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil. mariojac@icb.usp.br
Velasquez-Melendez
Gustavo
G
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000145:classification / Q000302:isolation & purification; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010510:Periodontal Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004269:DNA, Bacterial