title: High multidrug resistance protein activity in acute myeloid leukaemias is associated with poor response to chemotherapy and reduced patient survival.
abstract: Multidrug resistance protein (MRP) activity was investigated in 44 newly diagnosed acute myeloid leukaemia (AML) patients using a functional assay based on efflux of carboxy-2',7'-dichlorofluorescein, an anionic dye handled by both MRP1 and MRP2. Elevated MRP transport was detected in 29% of cases, but was not significantly correlated with sex, age, white blood cell count at diagnosis or karyotype. In contrast, it was associated with secondary AML (P = 0.002), CD34 positivity (P = 0.041) and P-glycoprotein activity (P = 0.01). There was a lower rate of complete remission in MRP-positive patients versus MRP-negative patients (23% versus 81%; P = 0.001); overall survival was also better for MRP-negative patients (P = 0.004). These data indicate a probable role for MRP activity in the clinical outcome of AML.
authors.affiliation: Laboratoire Universitaire d'Hématologie et de la Biologie des Cellules Sanguines, Faculté de Médecine, Rennes, France.
authors.forename: B
authors.initials: B
authors.lastname: Laupeze
authors.forename: L
authors.initials: L
authors.lastname: Amiot
authors.forename: B
authors.initials: B
authors.lastname: Drenou
authors.forename: M
authors.initials: M
authors.lastname: Bernard
authors.forename: B
authors.initials: B
authors.lastname: Branger
authors.forename: J M
authors.initials: JM
authors.lastname: Grosset
authors.forename: T
authors.initials: T
authors.lastname: Lamy
authors.forename: R
authors.initials: R
authors.lastname: Fauchet
authors.forename: O
authors.initials: O
authors.lastname: Fardel
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood* / Q000188:drug therapy / Q000401:mortality; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis*; D012074:Remission Induction; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D018952:Antigens, CD34; D015415:Biomarkers; D027425:ATP-Binding Cassette, Sub-Family C Proteins