Analysis of the role of RecQ helicases in RNAi in mammals.
The identity of mammalian genes involved in RNA interference (RNAi), the targeted sequence-specific mRNA degradation by double-stranded RNA (dsRNA), is poorly defined. Here we report the analysis of mice with null mutations of Wrn, Blm, and RecQ1 genes that are related to Mut-7 and Qde3, two genes essential for RNAi in Caenorhabditis elegans and quelling in Neurospora, respectively. Our results suggest that Wrn, Blm, and RecQ1 are not involved in sequence-specific mRNA degradation in mammals in response to dsRNA, suggesting potential differences in the mammalian RNAi pathway.
Stein
Paula
P
Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104-6018, USA.
Svoboda
Petr
P
Stumpo
Deborah J
DJ
Blackshear
Perry J
PJ
Lombard
David B
DB
Johnson
Brad
B
Schultz
Richard M
RM
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005090:Exodeoxyribonucleases; D005656:Fungal Proteins; D051379:Mice; D009491:Neurospora / Q000235:genetics; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053484:RecQ Helicases; D000071657:Werner Syndrome Helicase
D005656:Fungal Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D005090:Exodeoxyribonucleases; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; C506725:RECQL protein, human; D004265:DNA Helicases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase; C402647:QDE-3 protein, Neurospora crassa