Nucleotide sequence of envelope protein of Japanese encephalitis virus SA14-14-2 adapted to vero cells.
Live attenuated Japanese encephalitis (JE) virus SA(14)-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA(14)-14-2(Vero). Animal testing showed that SA(14)-14-2(Vero) has an attenuation phenotype similar to that of the parent SA(14)-14-2(PDK) strain in mice.
Hong
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Cheil Jedang Corporation, Research and Development Center, Ichon-Si, Kyeonggi-Do, South Korea.
Yoo
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WD
Putnak
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Eckels
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KH
Rho
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HM
Kim
S O
SO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D004285:Dogs; D004664:Encephalitis Virus, Japanese / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins