Screening of African medicinal plants for antimicrobial and enzyme inhibitory activity.
Seven plant species, belonging to different families, were collected in the eastern part of the Republic of Congo (Kivu) based on ethnopharmacological information. Their dichloromethane and methanolic extracts were tested for biological activity. Five of the seven collected plants exhibited antiplasmodial activity with IC(50) values ranging from 1.1 to 9.8 microg/ml. The methanolic extract of Cissampelos mucronata was the most active one showing activity against chloroquine sensitive (D6) and chloroquine resistant (W2) Plasmodium falciparum strains with IC(50) values of 1.5 and 1.1 microg/ml, respectively. Additionally, this extract significantly inhibited the enzyme tyrosine kinase p56(lck) (TK). The dichloromethane extract of Amorphophallus bequaertii inhibited the growth of Mycobacterium tuberculosis with a MIC of 100 microg/ml and the methanolic extract of Rubus rigidus inhibited the activity of both enzymes HIV1-reverse transcriptase (HIV1-RT) and TK p56(lck).
Tshibangu
Jeannette Ndaya
JN
Institute for Pharmaceutical Biology, Nussallee 6, D-53115, Bonn, Germany.
Chifundera
Kusamba
K
Kaminsky
Ronald
R
Wright
Anthony David
AD
König
Gabriele Maria
GM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003223:Congo; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006801:Humans; D007624:KB Cells; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D010963:Plasmodium falciparum / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D010936:Plant Extracts; D011505:Protein-Tyrosine Kinases; D054303:HIV Reverse Transcriptase