title: Plastid state- and light-dependent regulation of the expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis.
abstract: Interorganellar regulatory interactions in the flagellate Euglena gracilis were shown to be more complicated than in green algae and higher plants. Euglena plastids have a much more complex influence on nuclear gene expression than was previously thought. The petJ gene for cytochrome c6 represents a group of nucleus-encoded genes for chloroplast proteins, the expression of which is influenced by the state of plastids at the transcriptional level. Moroever, the regulation of these genes might be light-dependent. In contrast, for nucleus-encoded small subunit of ribulose-bisphosphate carboxylase, chlorophyll a/b binding protein, and porphobilinogen deaminase transcript levels are unchanged in wild-type cells relative to white mutants. In these cases there is no plastid-derived signal operative during transcription. Porphobilinogen deaminase appeared to be regulated even at the post-translational level.
authors.affiliation: Institute of Cell Biology, Comenius University, 811 07 Bratislava, Slovakia.
authors.forename: R
authors.initials: R
authors.lastname: Vacula
authors.forename: J M
authors.initials: JM
authors.lastname: Steiner
authors.forename: J
authors.initials: J
authors.lastname: Krajcovic
authors.forename: L
authors.initials: L
authors.lastname: Ebringer
authors.forename: W
authors.initials: W
authors.lastname: Löffelhardt
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D011163:Hydroxymethylbilane Synthase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D018087:Plastids / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D003574:Cytochrome c Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D011506:Proteins; D012333:RNA, Messenger; C083951:cytochrome c553; C088752:fucoxanthin-, chlorophyll a-c-containing protein; C022302:cytochrome C-552; D011163:Hydroxymethylbilane Synthase; D012273:Ribulose-Bisphosphate Carboxylase