title: Ribosome as a molecular machine.
abstract: General principles of structure and function of the ribosome are surveyed, and the translating ribosome is regarded as a molecular conveying machine. Two coupled conveying processes, the passing of compact tRNA globules and the drawing of linear mRNA chain through intraribosomal channel, are considered driven by discrete acts of translocation during translation. Instead of mechanical transmission mechanisms and power-stroke 'motors', thermal motion and chemically induced changes in affinities of ribosomal binding sites for their ligands (tRNAs, mRNA, elongation factors) are proposed to underlie all the directional movements within the ribosomal complex. The GTP-dependent catalysis of conformational transitions by elongation factors during translation is also discussed.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290, Moscow Region, Pushchino, Russia. spirin@vega.protres.ru
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Spirin
mesh_terms: D000818:Animals; D001692:Biological Transport; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D010445:Peptide Elongation Factors / Q000502:physiology*; D014176:Protein Biosynthesis / Q000502:physiology*; D011487:Protein Conformation; D012270:Ribosomes / Q000737:chemistry / Q000502:physiology*
chemical_list: D010445:Peptide Elongation Factors; D006160:Guanosine Triphosphate