H-2 hemizygous mutants from a heterozygous cell line: role of mitotic recombination.
Variants that no longer express an entire H-2 haplotype were readily isolated, by immunoselection with antisera directed against the haplotype, from an H-2b/H-2d heterozygous Friend leukemia cell line carrying a Robertsonian translocation of the chromosomes bearing the H-2 genetic region. These variants can be denoted as being of the phenotype H-2b- H-2d+ or H-2b+ H-2d-. Some of the H-2b- H-2d+ variants: (1) lack the restriction enzyme fragments characteristic of the missing H-2b haplotype, as assessed by Southern blot analysis; (2) express more cell surface H-2d antigens than wild-type cells, as assessed by flow microfluorimetry; and (3) appear to have become homozygous for the more active H-2d-linked allele at the Glyoxalase I locus. These variants thus seem to have lost genetic material corresponding to the H-2b haplotype and may have gained genetic material corresponding to the H-2d haplotype. These results are consistent with the possibility that these variants were generated by mitotic recombination.
Rajan
T V
TV
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA.
Halay
E D
ED
Potter
T A
TA
Evans
G A
GA
Seidman
J G
JG
Margulies
D H
DH
D000818:Animals; D015139:Blotting, Southern; D015870:Gene Expression; D014644:Genetic Variation; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D006579:Heterozygote; D007791:Lactoylglutathione Lyase / Q000235:genetics; D051379:Mice; D008938:Mitosis / Q000235:genetics / Q000276:immunology; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D006183:H-2 Antigens; D007791:Lactoylglutathione Lyase