Orexin B immunoreactive fibers and terminals innervate the sensory and motor neurons of jaw-elevator muscles in the rat.
The relationship between orexinergic terminals and the sensory and motor neurons of jaw-elevator muscles was examined by means of anti-orexin B (OXB) immunohistochemistry combined with horseradish peroxidase (HRP) retrograde tracing in the rat. HRP was initially injected into the jaw-elevator muscles; 48 h later the animals were sacrificed and fixed for HRP reaction and anti-OXB immunohistochemistry. OXB-like terminals were observed to distribute in the trigeminal mesencephalic nucleus (Vme) and the trigeminal motor nucleus (Vmo) where they closely contact the Vme neuronal somata and the Vmo neuronal somata and dendrites retrogradely labeled with HRP. The results of this study provide anatomical evidence of a direct OXB orexinergic innervation of the sensory and motor neurons controlling jaw-elevator muscles involved in mastication. Its functional significance related to the feeding behavior and bruxism is discussed.
Zhang
Jingdong
J
Department of Oral and Craniofacial Biological Sciences, University of Maryland Dental School, Baltimore, Maryland 21201, USA.
Luo
Pifu
P
D000818:Animals; D005260:Female; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008406:Masseter Muscle / Q000294:innervation*; D008636:Mesencephalon / Q000648:ultrastructure*; D009046:Motor Neurons / Q000648:ultrastructure*; D009411:Nerve Endings / Q000648:ultrastructure; D009412:Nerve Fibers / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000648:ultrastructure*; D009479:Neuropeptides / Q000032:analysis; D018377:Neurotransmitter Agents; D000068797:Orexins; D051381:Rats; D017207:Rats, Sprague-Dawley; D013703:Temporal Muscle / Q000294:innervation; D014276:Trigeminal Nerve; D014278:Trigeminal Nuclei / Q000648:ultrastructure*
D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D000068797:Orexins; D006735:Horseradish Peroxidase