Spontaneous nicking in the nontoxic-nonhemagglutinin component of the Clostridium botulinum toxin complex.
The nontoxic-nonhemagglutinin (NTNHA) component, in both isolated form and the neurotoxin (NT)/NTNHA complexed form, was prepared protease-free from toxin complexes produced by Clostridium botulinum type D strain 4947. NTNHA in both preparations was found to be spontaneously converted to the nicked NTNHA form leading to 15- and 115-kDa fragments with the excision of several amino acid residues at specific sites on SDS-PAGE during long-term incubation, while that of the NT/NTNHA/hemagglutinin complexed form remained unnicked single-chain polypeptides under the same conditions. Considering that the NTNHA preparation contained small amounts of the nicked form of NTNHA and the addition of trypsin accelerated the cleavage, it is speculated that a nicked form of NTNHA remaining after the purification and/or NTNHA itself catalyzes the cleavage of intact NTNHA.
Sagane
Yoshimasa
Y
Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan.
Watanabe
Toshihiro
T
Kouguchi
Hirokazu
H
Sunagawa
Hiroyuki
H
Obata
Shigehiro
S
Oguma
Keiji
K
Ohyama
Tohru
T
D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000737:chemistry / Q000378:metabolism*; D006388:Hemagglutinins / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D037102:Lectins; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D016415:Sequence Alignment; D014357:Trypsin / Q000737:chemistry
C405516:Clostridium botulinum L toxin; C080139:Clostridium botulinum M toxin; D006388:Hemagglutinins; D037102:Lectins; D046911:Macromolecular Substances; D009498:Neurotoxins; D011480:Protease Inhibitors; D014357:Trypsin; D001905:Botulinum Toxins