Rapid construction of adenoviral vectors by lambda phage genetics.
Continued improvements of adenoviral vectors require the investigation of novel genome configurations. Since adenovirus can be generated directly by transfecting packaging cell lines with viral genomes isolated from plasmid DNA, it is possible to separate genome construction from virus production. In this way failure to generate a virus is not associated with an inability to generate the desired genome. We have developed a novel lambda-based system that allows rapid modification of the viral genome by double homologous recombination in Escherichia coli. The recombination reaction and newly generated genome may reside in a recombination-deficient bacterial host for enhanced plasmid stability. Furthermore, the process is independent of any restriction endonucleases. The strategy relies on four main steps: (i) homologous recombination between an adenovirus cosmid and a donor plasmid (the donor plasmid carries the desired modification[s] and flanking regions of homology to direct its recombination into the viral genome); (ii) in vivo packaging of the recombinant adenoviral cosmids during a productive lambda infection; (iii) transducing a recombination-deficient E. coli lambda lysogen with the generated lysate (the lysogen inhibits the helper phage used to package the recombinant andenoviral cosmid from productively infecting and destroying the host bacteria); (iv) effectively selecting for the desired double-recombinant cosmid. Approximately 10,000 double-recombinant cosmids are recovered per reaction with essentially all of them being the correct double-recombinant molecule. This system was used to generate quickly and efficiently adenoviral genomes deficient in the E1/E3 and E1/E3/E4 regions. The basis of this technology allows any region of the viral genome to be readily modified for investigation of novel configurations.
McVey
Duncan
D
GenVec, Inc., Gaithersburg, Maryland 20878, USA. dmcvey@genvec.com
Zuber
Mohammed
M
Ettyreddy
Damodar
D
Brough
Douglas E
DE
Kovesdi
Imre
I
D000256:Adenoviridae / Q000235:genetics* / Q000472:pathogenicity; D017456:Adenovirus Early Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics*; D002460:Cell Line; D003360:Cosmids; D004926:Escherichia coli / Q000821:virology; D005822:Genetic Vectors; D010957:Plasmids; D011995:Recombination, Genetic; D019065:Virus Assembly
D017456:Adenovirus Early Proteins