An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle.
An improved polymerase chain reaction test has been developed to detect Tritrichomonas foetus, the causative agent of trichomoniasis in cattle. The test amplifies a region of the 5.8S ribosomal RNA gene of T. foetus, and it is simple, sensitive, and specific when compared with traditional methods to examine field samples.
Nickel
D Douglas
DD
Department of Biochemistry and Molecular Biology, Health Sciences Centre, 3330 Hospital Drive NW, University of Calgary, Calgary, Alberta T2N 4N1.
Olson
Merle E
ME
Schultz
Gilbert A
GA
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011528:Protozoan Infections / Q000175:diagnosis / Q000469:parasitology; D011529:Protozoan Infections, Animal; D016053:RNA, Protozoan / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016835:Tritrichomonas foetus / Q000235:genetics / Q000302:isolation & purification*
D016053:RNA, Protozoan