title: Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen.
abstract: Brucella abortus RB51 is a rough (R) stable vaccine strain used in cattle and is believed to be devoid of O-side chain. We analyzed by use of a panel of monoclonal antibodies (MAbs) directed against seven previously defined O-polysaccharide (O-PS) epitopes the O-chain expression in strain RB51. Two MAbs specific for the C/Y (A=M) and C (M>A) epitopes showed low bindings in ELISA to strain RB51. O-chain expression was further confirmed by Western blot after SDS-PAGE of strain RB51. In particular, the MAb of C (M>A) specificity, showing preferential binding to M-dominant smooth (S) Brucella strains, revealed in strain RB51 a typical smooth-lipopolysaccharide (S-LPS) pattern which resembled that of M-dominant S-LPS. Thus, the results clearly show that strain RB51 produces low levels of M-like O-antigen.
authors.affiliation: Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France. cloeckae@tours.inra.fr
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
authors.forename: Michel S
authors.initials: MS
authors.lastname: Zygmunt
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Guilloteau
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002004:Brucella Vaccine / Q000276:immunology / Q000494:pharmacology; D002003:Brucella abortus / Q000235:genetics / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology* / Q000517:prevention & control; D002417:Cattle; D005260:Female; D005798:Genes, Bacterial; D019081:O Antigens / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D002004:Brucella Vaccine; D019081:O Antigens