title: Immunopeptides in the defense reactions of Glossina morsitans to bacterial and Trypanosoma brucei brucei infections.
abstract: Several dipteran insects are vectors of parasites causing major human infectious diseases. Among these, the tsetse fly, Glossina spp., is responsible for the transmission of trypanosomes, the pathogens responsible for sleeping sickness in Africa. A better understanding of insect-parasite interactions will help establish new strategies to fight this important often fatal disease. Antimicrobial peptides (AMPs) are part of the humoral immune response in insects during bacterial, fungal and parasitic infections. Here, we studied the immune response of Glossina morsitans to bacteria and to Trypanosoma brucei brucei by analyzing the synthesis of AMPs as markers of the humoral immune response. By reversed-phase chromatography, mass spectrometry analysis, Edman degradation and in vitro antimicrobial assays of the hemolymph of immune-challenged adults of G. morsitans, we identified three AMPs: a cecropin, an attacin and a defensin. These three AMPs were found to be induced upon systemic bacterial infection and also after per os infections by bacteria and parasites.
authors.affiliation: Réponse Immunitaire et Développement chez les Insectes, UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67000, Strasbourg, France. N.Boulanger@ibmc.u-strasbg.fr
authors.forename: N
authors.initials: N
authors.lastname: Boulanger
authors.forename: R
authors.initials: R
authors.lastname: Brun
authors.forename: L
authors.initials: L
authors.lastname: Ehret-Sabatier
authors.forename: C
authors.initials: C
authors.lastname: Kunz
authors.forename: P
authors.initials: P
authors.lastname: Bulet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D019476:Insect Proteins / Q000494:pharmacology*; D016982:Micrococcus luteus / Q000187:drug effects*; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*; D014370:Tsetse Flies
chemical_list: D000900:Anti-Bacterial Agents; D019476:Insect Proteins; D010455:Peptides; D014344:Trypanocidal Agents