title: Cornea procurement from very old donors: post organ culture cornea outcome and recipient graft outcome.
abstract: OBJECTIVE
  To study the suitability of corneas from very old donors for graft after banking and their clinical and endothelial outcomes in recipients.
  
  
  METHODS
  419 corneas stored in organ culture were divided into group 1, donors under 85 years (330 corneas) and group 2, "very old" donors aged 85 years and over (89 corneas). Endothelial cell density (ECD) before and after organ culture, discard rate before and after storage, and clinical and endothelial outcomes of the 196 penetrating keratoplasties (PKP) (158 in group 1 and 38 in group 2) were compared in a prospective longitudinal study.
  
  
  RESULTS
  Initial ECD was lower in group 2 than in group 1 and elimination for low ECD was more frequent in group 2 (respectively 38% v 20.2%, p=0.001). At the end of storage, because very old corneas lost fewer ECs than younger ones (respectively 4.2% v 9.5%, p=0.022), ECD was comparable between the two groups. The corneas of very old donors had a poorer macroscopic appearance at procurement and during surgery. Despite this, in grafted patients, overall graft survival in groups 1 and 2 (respectively 87.4% v 80.6%, p=0.197), visual acuity, and ECD did not differ at completion of the study (mean follow up 25 months).
  
  
  CONCLUSIONS
  This study suggests that endothelial cell count during banking ensures that functional and cellular results of PKPs are not dramatically influenced by very old donor age. Considering Europe's ageing population, the very elderly should not be deemed off limits for corneal procurement.
authors.affiliation: Department of Ophthalmology, Bellevue Hospital, University of Saint-Etienne, France. philippe.gain@univ-st-etienne.fr
authors.forename: P
authors.initials: P
authors.lastname: Gain
authors.forename: G
authors.initials: G
authors.lastname: Thuret
authors.forename: C
authors.initials: C
authors.lastname: Chiquet
authors.forename: P
authors.initials: P
authors.lastname: Rizzi
authors.forename: J L
authors.initials: JL
authors.lastname: Pugniet
authors.forename: S
authors.initials: S
authors.lastname: Acquart
authors.forename: J J
authors.initials: JJ
authors.lastname: Colpart
authors.forename: J C
authors.initials: JC
authors.lastname: Le Petit
authors.forename: J
authors.initials: J
authors.lastname: Maugery
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D003315:Cornea; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery; D016039:Corneal Transplantation / Q000379:methods*; D004728:Endothelium, Corneal; D005260:Female; D006085:Graft Survival; D006801:Humans; D015948:Keratoplasty, Penetrating / Q000379:methods; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D011446:Prospective Studies; D009927:Tissue and Organ Procurement / Q000458:organization & administration*; D016896:Treatment Outcome