title: The vitelliform macular dystrophy protein defines a new family of chloride channels.
abstract: Vitelliform macular dystrophy (VMD/Best disease; MIM*153700) is an early-onset autosomal dominant disorder in which accumulation of lipofuscin-like material within and beneath the retinal pigment epithelium is associated with a progressive loss of central vision. Bestrophin, the protein product of the VMD gene, has four predicted transmembrane domains. There are multiple bestrophin homologues in the human, Drosophila, and Caenorhabditis elegans genomes, but no function has previously been ascribed to these proteins, and they show no detectable homology to other proteins of known function. Using heterologous expression, we show here that human, Drosophila, and C. elegans bestrophins form oligomeric chloride channels, and that human bestrophin is sensitive to intracellular calcium. Each of 15 missense mutations asscociated with VMD greatly reduces or abolishes the membrane current. Four of these mutant bestrophins were coexpressed with the wild type and each dominantly inhibited the wild-type membrane current, consistent with the dominant nature of the disease. These experiments establish the existence of a new chloride channel family and VMD as a channelopathy.
authors.affiliation: Department of Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsunenari
authors.forename: King-Wai
authors.initials: KW
authors.lastname: Yau
authors.forename: Jeremy
authors.initials: J
authors.lastname: Nathans
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D000075002:Bestrophins; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D018118:Chloride Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D004553:Electric Conductivity; D005136:Eye Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005799:Genes, Dominant / Q000235:genetics; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects; D008958:Models, Molecular; D020125:Mutation, Missense / Q000235:genetics; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D012162:Retinal Degeneration / Q000235:genetics*
chemical_list: C113133:BEST1 protein, human; D000075002:Bestrophins; D029742:Caenorhabditis elegans Proteins; D018118:Chloride Channels; D002712:Chlorides; D029721:Drosophila Proteins; D005136:Eye Proteins; D021122:Protein Subunits; D003545:Cysteine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002118:Calcium