title: Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood.
abstract: During ageing thymic function declines and is unable to meet the demand for peripheral T helper (Th) cell replenishment. Therefore, population maintenance of naive Th cells must be at least partly peripherally based. Such peripheral postthymic expansion of recent thymic emigrants (RTEs) during ageing consequently should lead to loss or dilution of T cell receptor excision circles (TRECs) from a subset of naive T cells. We have identified two subsets of naive Th cells in human adult peripheral blood characterized by a striking unequal content of TRECs, indicating different peripheral proliferative histories. TRECs are highly enriched in peripheral naive CD45RA(+) Th cells coexpressing CD31 compared with peripheral naive CD45RA(+) Th cells lacking CD31 expression, in which TRECs can hardly be detected. Furthermore we show that CD31(-)CD45RA(+) Th cells account for increasing percentages of the naive peripheral Th cell pool during ageing but retain phenotypic and functional features of naive Th cells. As CD31 is lost upon T cell receptor (TCR) engagement in vitro, we hypothesize that TCR triggering is a prerequisite for homeostatically driven peripheral postthymic expansion of human naive RTEs. We describe here the identification of peripherally expanded naive Th cells in human adult blood characterized by the loss of CD31 expression and a highly reduced TREC content.
authors.affiliation: Department of Clinical Immunology, Deutsches Rheuma-Forschungszentrum Berlin, Schumannstrasse 21/22, 10117 Berlin, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kimmig
authors.forename: Grzegorz K
authors.initials: GK
authors.lastname: Przybylski
authors.forename: Christian A
authors.initials: CA
authors.lastname: Schmidt
authors.forename: Katja
authors.initials: K
authors.lastname: Laurisch
authors.forename: Beate
authors.initials: B
authors.lastname: Möwes
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
authors.forename: Andreas
authors.initials: A
authors.lastname: Thiel
mesh_terms: D000328:Adult; D000375:Aging / Q000276:immunology*; D018952:Antigens, CD34 / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens