An integrated physical and genetic map of the rice genome.
Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome fingerprint-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome ( approximately 90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes.
Chen
Mingsheng
M
Clemson University Genomics Institute, 100 Jordan Hall, Clemson, South Carolina 29634-5727, USA.
Presting
Gernot
G
Barbazuk
W Brad
WB
Goicoechea
Jose Luis
JL
Blackmon
Barbara
B
Fang
Guangchen
G
Kim
Hyeran
H
Frisch
David
D
Yu
Yeisoo
Y
Sun
Shouhong
S
Higingbottom
Stephanie
S
Phimphilai
John
J
Phimphilai
Dao
D
Thurmond
Scheen
S
Gaudette
Brian
B
Li
Ping
P
Liu
Jingdong
J
Hatfield
Jamie
J
Main
Dorrie
D
Farrar
Kasey
K
Henderson
Caroline
C
Barnett
Laura
L
Costa
Ravi
R
Williams
Brian
B
Walser
Suzanne
S
Atkins
Michael
M
Hall
Caroline
C
Budiman
Muhammad A
MA
Tomkins
Jeffery P
JP
Luo
Meizhong
M
Bancroft
Ian
I
Salse
Jerome
J
Regad
Farid
F
Mohapatra
Trilochan
T
Singh
Nagendra K
NK
Tyagi
Akhilesh K
AK
Soderlund
Carol
C
Dean
Ralph A
RA
Wing
Rod A
RA
D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D019295:Computational Biology; D020451:Contig Mapping / Q000379:methods; D020732:Cytogenetic Analysis; D016172:DNA Fingerprinting; D015723:Gene Library; D005819:Genetic Markers; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D011995:Recombination, Genetic
D005819:Genetic Markers