title: Characterization of plant proteins that interact with cowpea mosaic virus '60K' protein in the yeast two-hybrid system.
abstract: Cowpea mosaic virus (CPMV) replication occurs in close association with small membranous vesicles in the host cell. The CPMV RNA1-encoded 60 kDa nucleotide-binding protein ('60K') plays a role in the formation of these vesicles. In this study, five cellular proteins were identified that interacted with different domains of 60K using a yeast two-hybrid search of an Arabidopsis cDNA library. Two of these host proteins (termed VAP27-1 and VAP27-2), with high homology to the VAP33 family of SNARE-like proteins from animals, interacted specifically with the C-terminal domain of 60K and upon transient expression colocalized with 60K in CPMV-infected cowpea protoplasts. eEF1-beta, picked up using the central domain of 60K, was also found to colocalize with 60K. The possible role of these host proteins in the viral replicative cycle is discussed.
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Jan E
authors.initials: JE
authors.lastname: Carette
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Jan
authors.initials: J
authors.lastname: Verver
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Joost
authors.initials: J
authors.lastname: Martens
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Tony
authors.initials: T
authors.lastname: van Kampen
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Joan
authors.initials: J
authors.lastname: Wellink
authors.affiliation: Laboratory of Molecular Biology, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands1.
authors.forename: Ab
authors.initials: A
authors.lastname: van Kammen
mesh_terms: D015153:Blotting, Western; D002736:Chloroplasts / Q000378:metabolism; D017798:Comovirus / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins