title: Multibiomarker responses in fish from the Moselle River (France).
abstract: The response of wild fish to pollutants was studied using two biomarkers in chub (Leuciscus cephalus) at five stations in the Moselle River (France) in 1998 and in 1999. The induction of cytochrome P450 1A was quantified by the ethoxyresorufin O-deethylase (EROD) activity in the liver and the level of DNA single-strand breaks was determined in erythrocytes using the comet assay. EROD activity was observed to be up to 10-fold induced in both males and females from the downstream stations in comparison to the fish from the upstream station. Levels of DNA damage did not parallel EROD induction. Chemical analyses did not clearly explain the responses of the studied biomarkers, confirming the great difficulty in relating chemical and biological information in the field. This study confirms the difficulty in assessing the biological effects of mixtures of pollutants and points out the usefulness of a large array of biomarkers.
authors.affiliation: Cemagref, Division Biologie des Ecosystèmes Aquatiques, 3 bis Quai Chauveau CP 220, 69336 Lyon cedex 9, France. patrick.flammarion@cemagref.fr
authors.forename: P
authors.initials: P
authors.lastname: Flammarion
authors.forename: A
authors.initials: A
authors.lastname: Devaux
authors.forename: S
authors.initials: S
authors.lastname: Nehls
authors.forename: B
authors.initials: B
authors.lastname: Migeon
authors.forename: P
authors.initials: P
authors.lastname: Noury
authors.forename: J
authors.initials: J
authors.lastname: Garric
mesh_terms: D000818:Animals; D015415:Biomarkers; D020552:Comet Assay; D003530:Cyprinidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D004249:DNA Damage; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D005602:France; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1