title: Kinetic analysis of PPi-dependent phosphofructokinase from Porphyromonas gingivalis.
abstract: We have previously cloned the gene encoding a pyrophosphate-dependent phosphofructokinase (PFK), designated PgPFK, from Porphyromonas gingivalis, an oral anaerobic bacterium implicated in advanced periodontal disease. In this study, recombinant PgPFK was purified to homogeneity, and biochemically characterized. The apparent K(m) value for fructose 6-phosphate was 2.2 mM, which was approximately 20 times higher than that for fructose 1,6-bisphosphate. The value was significantly greater than any other described PFKs, except for Amycolatopsis methanolica PFK which is proposed to function as a fructose 1,6 bisphosphatase (FBPase). The PgPFK appears to serves as FBPase in this organism. We postulate that this may lead to the gluconeogenic pathways to synthesize the lipopolysaccharides and/or glycoconjugates essential for cell viability.
authors.affiliation: Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu 803-8580, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Arimoto
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Ansai
authors.forename: Weixian
authors.initials: W
authors.lastname: Yu
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Turner
authors.forename: Tadamichi
authors.initials: T
authors.lastname: Takehara
mesh_terms: D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D016966:Porphyromonas gingivalis / Q000201:enzymology*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D010770:Phosphotransferases; C022502:pyrophosphate-fructose 6-phosphate 1-phosphotransferase