[Cloning of HAL1 gene and characterization for salt tolerance tomato].
The HAL1 gene was cloned by PCR strategy and confirmed by sequencing. Its open read frame is 879 bp, encoding a peptide of 294 amino acids (32 kD Protein). A chimaeric construct of HAL1 and Npt II (neomycin phosphotransferase II) was constructed and introduced into commercial cultivars of tomato (Zhong SU No. 5: Lycopersicon escullentum) by Agrobacterium tumefacien-mediated gene transformation. Transformants were selected for their ability to grow and root on media containing kanamycin. Transformation was confirmed by analysis of PCR, Southern blot and RT-PCR. The salt tolerance of transgenic tomato is evaluated by comparing the fresh weight, dry weight, Na+, K+ content of transgenic tomato and control tomato. It is concluded that the over-expressing of HAL1 in tomato could enhance the salt tolerance of the transgenic tomato.
Zhang
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Q
Plant Stress Key Lab, Shandong Normal University, Jinan 250014, China.
Wang
S F
SF
Zhao
Y X
YX
Zhao
K F
KF
Zhang
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H
D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D019868:Kanamycin Kinase / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride / Q000378:metabolism*; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics
D005656:Fungal Proteins; C076232:HAL1 protein, S cerevisiae; D047908:Intracellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D019868:Kanamycin Kinase