Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus core, within a lipid bilayer, has a less-ordered structure than the external, icosahedral scaffold of 90 glycoprotein E dimers. The three E monomers per icosahedral asymmetric unit do not have quasiequivalent symmetric environments. Difference maps indicate the location of the small membrane protein M relative to the overlaying scaffold of E dimers. The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane.
Kuhn
Richard J
RJ
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. rjkuhn@bragg.bio.purdue.edu
Zhang
Wei
W
Rossmann
Michael G
MG
Pletnev
Sergei V
SV
Corver
Jeroen
J
Lenches
Edith
E
Jones
Christopher T
CT
Mukhopadhyay
Suchetana
S
Chipman
Paul R
PR
Strauss
Ellen G
EG
Baker
Timothy S
TS
Strauss
James H
JH
D002213:Capsid / Q000737:chemistry; D020285:Cryoelectron Microscopy; D003716:Dengue Virus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008051:Lipid Bilayers / Q000737:chemistry; D008561:Membrane Fusion / Q000502:physiology; D008958:Models, Molecular; D014759:Viral Envelope Proteins / Q000737:chemistry
D008051:Lipid Bilayers; D014759:Viral Envelope Proteins