title: CD30 and the NPM-ALK fusion protein (p80) are differentially expressed between peripheral blood and bone marrow in primary small cell variant of anaplastic large cell lymphoma.
abstract: The t(2;5)(p23;q35) translocation results in the formation of a unique chimeric NPM-ALK protein (p80). Expression of this protein is considered to be one of the clinical features of anaplastic large cell lymphoma (ALCL). Recently recognized as one clinical subtype of ALCL, the small cell variant is prone to have a leukemic presentation. Although the small cell variant has been recognized as a subtype of ALCL, the clinical properties of this subtype, especially the immunophenotype of lymphoma cells in peripheral blood, have not yet been fully described. This report shows that neither CD30 nor p80 is detected by immunostaining in the predominant small cell malignant clone and also in large lymphoma cells in peripheral blood, while large cells and occasionally observed small cells in bone marrow were found to be positive for CD30 and p80. Our findings suggest that differential expression of CD30 and p80 between peripheral blood and bone marrow lymphoma cells is a property of the small cell variant of ALCL.
authors.affiliation: The Second Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, Saitama, Japan. nawaya@fhcrc.org
authors.forename: Norihiro
authors.initials: N
authors.lastname: Awaya
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Mori
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Mori
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sugano
authors.forename: Tamihiro
authors.initials: T
authors.lastname: Kamata
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Toru
authors.initials: T
authors.lastname: Abe
mesh_terms: D001773:Blood Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D001854:Bone Marrow Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D048429:Cell Size; D002889:Chromosomes, Human, Pair 2; D002895:Chromosomes, Human, Pair 5; D020732:Cytogenetic Analysis; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000032:analysis / Q000378:metabolism*; D017728:Lymphoma, Large-Cell, Anaplastic / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000378:metabolism*; D014178:Translocation, Genetic
chemical_list: D017730:Ki-1 Antigen; C107023:p80(NPM-ALK) protein; D011505:Protein-Tyrosine Kinases