PD-loop technology: PNA openers at work.
A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based assays over existing techniques for sequence-specific detection and manipulation of DNA duplexes are discussed. Future prospects for the further development of PD-loop technology are highlighted.
Demidov
V V
VV
Center for Advanced Biotechnology, Department of Biomedical Engineering, Boston University, 36 Cummington Street, Boston, MA 02215, USA. vvd@bu.edu
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D018076:DNA, Complementary; D006801:Humans; D008956:Models, Chemical; D008957:Models, Genetic; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004247:DNA