[Changes of mRNA expression of IL-1beta and TNF-alpha in rat brains after repeated exposures to +Gz].
Objective. To study changes of mRNA expression of IL-1beta and TNF-alpha in rat brains after repeated exposures to +Gz. Method. Twenty conscious SD rats served as the subjects. They were randomly divided into 5 groups. Using an animal centrifuge, control rats (n = 4) were exposed to +1 Gz and experimental rats (n = 16) were exposed to +14 Gz three times, each for 45 s with 30 min interval in between. The rat brains were taken 30 min, 6 h, 24 h and 48 h after the last centrifuge run and total RNA was isolated. mRNA expression levels of IL-1beta and TNF-alpha in rat brain were measured by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Result. mRNA expression levels of IL-1beta and TNF-alpha in rat brains taken 30 min, 6 h and 24 h after repeated +Gz exposures were significantly higher than those in control rats, but returned to normal after 48 h. Conclusion. It suggested that mRNA expressions of IL-1beta and TNF-alpha in rat brains can be stimulated by repeated +Gz exposures and the increased expression of IL-1beta and TNF-alpha may play a role in the pathologic course of brain damage induced by +Gz exposures, but the damage is reversible.
Liu
H J
HJ
Clinical Aeromedical Center, General Hospital of the Air Force, Beijing, China.
Cai
Q
Q
Ji
G Y
GY
Zhan
Z
Z
Jiang
J D
JD
Zhu
M C
MC
D000054:Acceleration; D000818:Animals; D001921:Brain / Q000378:metabolism; D002498:Centrifugation; D005786:Gene Expression Regulation; D018471:Hypergravity; D007375:Interleukin-1 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha