The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.
The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.
Rosa
F
F
Institut Pasteur, Université Paris VII, France.
Berissi
H
H
Weissenbach
J
J
Maroteaux
L
L
Fellous
M
M
Revel
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002051:Burkitt Lymphoma; D003001:Cloning, Molecular; D018387:Codon, Initiator; D018076:DNA, Complementary; D004926:Escherichia coli; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D001613:beta 2-Microglobulin / Q000235:genetics*
D018387:Codon, Initiator; D018076:DNA, Complementary; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D001613:beta 2-Microglobulin