Cryopreservation of cultivated and wild Arachis species embryonic axes using desiccation and vitrification methods.
The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.
Gagliardi
R F
RF
Laboratório de Micropropagação e Transformação de Plantas, Universidade do Estado do Rio de Janeiro, Brazil.
Pacheco
G P
GP
Valls
J F M
JF
Mansur
E
E
D010367:Arachis / Q000196:embryology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D003890:Desiccation / Q000379:methods*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009584:Nitrogen; D018520:Plant Shoots / Q000254:growth & development; D020127:Recovery of Function; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*; D013395:Sucrose / Q000494:pharmacology
D003451:Cryoprotective Agents; D013395:Sucrose; D009584:Nitrogen; D005990:Glycerol; D004121:Dimethyl Sulfoxide