title: High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice.
abstract: We report the development of a robust and effective method for multiplexed short tandem repeat (STR) analysis within a chip-based microdevice. The method uses a laser-induced fluorescence detection system and simultaneously detects three- and four-color multiplexed polymerase chain reaction (PCR) samples. Analyses of the eight combined DNA index system (CODIS) STR loci were performed in 20 min with single-base-pair resolution ranging from 0.75 to 1. A simultaneous analysis of fifteen loci-ladders and a gender marker Amelogenin based on the PowerPlexTM 16 System was achieved in less than 35 min. The system is capable of repetitive operation and may be extended to high-throughput multilane devices that could be readily interfaced to an automated sample loading system.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors.forename: Luba
authors.initials: L
authors.lastname: Mitnik
authors.forename: Loucinda
authors.initials: L
authors.lastname: Carey
authors.forename: Robert
authors.initials: R
authors.lastname: Burger
authors.forename: Samantha
authors.initials: S
authors.lastname: Desmarais
authors.forename: Lance
authors.initials: L
authors.lastname: Koutny
authors.forename: Oliver
authors.initials: O
authors.lastname: Wernet
authors.forename: Paul
authors.initials: P
authors.lastname: Matsudaira
authors.forename: Daniel
authors.initials: D
authors.lastname: Ehrlich
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D008832:Microchemistry; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences