The transcriptional map of the common eliminated region 1 (C3CER1) in 3p21.3.
Occurrence of chromosome 3p deletions in a large number of human tumours suggests the existence of uncharted tumour suppressor gene(s). We previously applied a functional assay, named the Elimination test (Et), for the identification of regions containing tumour growth antagonising genes. This resulted in the definition of chromosome 3 common eliminated region 1 (C3CER1) on 3p21.3, which is regularly eliminated from SCID-derived tumours. Systematic genomic sequencing of 11 PAC clones, combined with comparisons of genomic sequence against EST databases and PCR-based cloning of cDNA sequences allowed us to assemble a comprehensive transcriptional map of 1.4 Mb that includes 19 active genes and three processed pseudogenes. We report four novel genes: FYVE and coiled-coil domain containing 1 (FYCO1), transmembrane protein 7 (TMEM7), leucine-rich repeat-containing 2 (LRRC2) and leucine zipper protein 3 (LUZP3). A striking feature of C3CER1 is a presence of a cluster of eight chemokine receptor genes. Based on a new analysis of the microcell hybrid-derived panel of SCID tumours we also redefined the centromeric border of the C3CER1. It is now located within LRRC2 gene, which is a relative of RSP-1 (Ras Suppressor Protein 1). The detailed knowledge of gene content in C3CER1 is a prerequisite for functional analysis of these genes and understanding of their possible role in tumorigenesis.
Kiss
Hajnalka
H
Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, S-17177, Stockholm, Sweden. Hajnalka.Kiss@mtc.ki.se
Yang
Ying
Y
Kiss
Csaba
C
Andersson
Kenth
K
Klein
George
G
Imreh
Stephan
S
Dumanski
Jan P
JP
D002503:Centromere / Q000235:genetics; D002893:Chromosomes, Human, Pair 3; D004268:DNA-Binding Proteins / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D020161:Physical Chromosome Mapping; D011544:Pseudogenes; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; C460141:FYCO1 protein, human; D008869:Microtubule-Associated Proteins; D014157:Transcription Factors