How does tmRNA move through the ribosome?
To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon-anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.
Ivanov
Pavel V
PV
Department of Chemistry, M.V. Lomonosov Moscow State University, 119899, Moscow, Russia.
Zvereva
Maria I
MI
Shpanchenko
Olga V
OV
Dontsova
Olga A
OA
Bogdanov
Alexey A
AA
Aglyamova
Galina V
GV
Lim
Valery I
VI
Teraoka
Yoshika
Y
Nierhaus
Knud H
KH
D017123:Bacteriophage T7 / Q000235:genetics; D001692:Biological Transport; D004926:Escherichia coli / Q000235:genetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*
D012329:RNA, Bacterial; C096222:tmRNA