Transient receptor potential 1 regulates capacitative Ca(2+) entry and Ca(2+) release from endoplasmic reticulum in B lymphocytes.
Capacitative Ca(2+) entry (CCE) activated by release/depletion of Ca(2+) from internal stores represents a major Ca(2+) influx mechanism in lymphocytes and other nonexcitable cells. Despite the importance of CCE in antigen-mediated lymphocyte activation, molecular components constituting this mechanism remain elusive. Here we demonstrate that genetic disruption of transient receptor potential (TRP)1 significantly attenuates both Ca(2+) release-activated Ca(2+) currents and inositol 1,4,5-trisphosphate (IP(3))-mediated Ca(2+) release from endoplasmic reticulum (ER) in DT40 B cells. As a consequence, B cell antigen receptor-mediated Ca(2+) oscillations and NF-AT activation are reduced in TRP1-deficient cells. Thus, our results suggest that CCE channels, whose formation involves TRP1 as an important component, modulate IP(3) receptor function, thereby enhancing functional coupling between the ER and plasma membrane in transduction of intracellular Ca(2+) signaling in B lymphocytes.
Mori
Yasuo
Y
Center for Integrative Bioscience, Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji-cho, Okazaki 444-8585, Japan. moriy@nips.ac.jp
Wakamori
Minoru
M
Miyakawa
Tomoya
T
Hermosura
Meredith
M
Hara
Yuji
Y
Nishida
Motohiro
M
Hirose
Kenzo
K
Mizushima
Akiko
A
Kurosaki
Mari
M
Mori
Emiko
E
Gotoh
Kumiko
K
Okada
Takaharu
T
Fleig
Andrea
A
Penner
Reinhold
R
Iino
Masamitsu
M
Kurosaki
Tomohiro
T
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000235:genetics; D002460:Cell Line; D002645:Chickens; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017353:Gene Deletion; D006801:Humans; D050052:TRPC Cation Channels
D015220:Calcium Channels; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D002118:Calcium