title: The effect of partial vitrectomy on blood-ocular barrier function in the rabbit.
abstract: OBJECTIVE
  To compare ocular vascular permeability in the rabbit after vitrectomy as assessed by contrast-enhanced magnetic imaging (CE-MRI) and measurements of aqueous and vitreous humor protein concentration.
  
  
  METHODS
  Partial vitrectomies were performed, irrigating with BSS or BSS PLUS. Post-operative vascular leakage was determined by CE-MRI following intravenous administration of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA). Aqueous and vitreous protein concentrations were quantified by standard biochemical assay. ERG evaluations were performed on postoperative days 1, 3, and 7.
  
  
  RESULTS
  Using BSS as irrigant, breakdown of the inner blood-retinal barrier (BRB) occurred in 4/7 eyes on post-operative day 1. The rate of Gd-DTPA leakage was significantly greater on postoperative day 1 than that in unoperated, control eyes, but declined approximately 50% by day 3. At both time points, outer BRB breakdown was restricted to the sclerotomy wounds. No BRB leakage was detectable in control eyes. Blood-aqueous barrier (BAB) leakage was bilateral on day 1. Significantly greater Gd-DTPA leakage occurred in the operated eye than in the nonsurgical contralateral eye. On day 3, approximately 40% bilateral reduction in leakage indicated resolution of BAB leakage. Notably, Gd-DTPA leakage of the BAB and BRB was significantly reduced in the BSS PLUS treated group. In contrast to MRI assessments, protein concentrations of the aqueous and vitreous in the surgical eye showed no detectable differences between BSS and BSS PLUS. Concurrent with the transient loss of ocular barrier function, ERG responses also declined. However, by day 7 greater than 90% recovery was noted in BSS PLUS treated animals but not in the BSS treatment group.
  
  
  CONCLUSIONS
  CE-MRI is capable of detecting subtle changes in vascular permeability following ocular surgery. Advantages of using BSS PLUS compared to BSS as the irrigating solution can be detected using this technique. BSS PLUS's protection of barrier function is consistent with a rapid recovery in retinal function not observed in BSS treated eyes.
authors.affiliation: Alcon Research, Ltd., Fort Worth, TX 76134, USA.
authors.forename: W H
authors.initials: WH
authors.lastname: Garner
authors.forename: S
authors.initials: S
authors.lastname: Scheib
authors.forename: B A
authors.initials: BA
authors.lastname: Berkowitz
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: C A
authors.initials: CA
authors.lastname: Wilson
authors.forename: G
authors.initials: G
authors.lastname: Graff
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D018916:Blood-Aqueous Barrier / Q000502:physiology*; D001813:Blood-Retinal Barrier / Q000502:physiology*; D002199:Capillary Permeability / Q000502:physiology; D003287:Contrast Media; D004596:Electroretinography; D019786:Gadolinium DTPA; D008279:Magnetic Resonance Imaging; D011817:Rabbits; D012160:Retina / Q000502:physiology; D012171:Retinal Vessels / Q000502:physiology; D014821:Vitrectomy; D014822:Vitreous Body / Q000378:metabolism
chemical_list: D003287:Contrast Media; D019786:Gadolinium DTPA