Levels of thermostable direct hemolysin produced by Vibrio parahaemolyticus O3:K6 and other serovars grown anaerobically with the presence of a bile acid.
Twenty-three V. parahaemolyticus strains, including 12 pandemic O3:K6 strains, were examined for their growth and production of thermostable direct hemolysin (TDH) under an anaerobic culture condition with or without presence of a bile acid, taurocholic acid (TCA). Both bacterial growth and TDH production were markedly enhanced by TCA for a majority of the strains, but the scale of the TDH production was disproportionately greater than that of the corresponding growth for 14 strains. Such enhancement was, however, not specific to the pandemic strains.
Osawa
Ro
R
Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1-I, Nada-ku, Kobe, 657-8501, Japan.
Arakawa
Eiji
E
Okitsu
Tadayuki
T
Yamai
Shiro
S
Watanabe
Haruo
H
D000332:Aerobiosis; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001647:Bile Acids and Salts; D003470:Culture Media; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005838:Genotype; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006801:Humans; D007214:Indonesia; D007564:Japan; D007723:Korea; D012623:Seawater; D013656:Taurocholic Acid; D013785:Thailand; D014481:United States; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D001427:Bacterial Toxins; D001647:Bile Acids and Salts; D003470:Culture Media; D006460:Hemolysin Proteins; C068489:thermostable direct hemolysin; D013656:Taurocholic Acid