title: Dynamics of bone loss in experimental periodontitis.
abstract: A dynamic histomorphometric study of bone loss in periodontitis induced by inserting a thread ligature around the neck of the lower first molar of Wistar rats weighing 300 g. was performed. Bone formation fronts were labelled twice by tetracycline injection (day 1 and day 14). On day 16 the animals were divided into 4 groups: Two experimental (ligature in place) and two controls (no ligature). Animals in one experimental and one control groups were killed 72 hours post insertion, and the other two groups 96 hours post-insertion. Grinding sections of the first molar were obtained to perform histomorphometric studies on microphotographs taken under fluorescence microscopy. At 72 hours results showed total loss of the double labeling in the mesial wall, partial loss in the top of the crest and no loss in the distal wall. At 96 hours, loss of the double labeling at the top of the crest was greater, while only one label (the first) could be observed in the distal wall. These results show that in this periodontitis experimental model, bone loss is initiated and is more rapid in the bone remodeling (mesial) wall than in the modeling (distal) wall. This understanding of bone loss dynamics enables the characterization of the model employed herein, contributing to further studies on the course of periodontal disease under different experimental conditions.
authors.affiliation: Department of Histology, School of Dentistry, University of Buenos Aires and Department of Rediobiology, National Atomic Energy Commission, Buenos Aires, Argentina.
authors.forename: P M
authors.initials: PM
authors.lastname: Mandalunis
authors.forename: O R
authors.initials: OR
authors.lastname: Costa
authors.forename: A M
authors.initials: AM
authors.lastname: Ubios
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D015519:Bone Density; D018450:Disease Progression; D008026:Ligation; D008297:Male; D008856:Microscopy, Fluorescence; D010518:Periodontitis / Q000150:complications*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors