title: Segregation of Bad from lipid rafts is implicated in the induction of apoptosis.
abstract: Many molecules relocate subcellularly in cells undergoing apoptosis. Using coimmunoprecipitation experiments we demonstrate that Bad is not associated to 14-3-3 protein, suggesting a new mechanism for the control of the proapoptotic role of Bad. Here we show, by confocal microscopy and cellular fractionation, that Bad is attached to lipid rafts in IL-4-stimulated cells and thymocytes while associated with mitochondria in IL-4-deprived cells. Disruption of lipid rafts by methyl-beta-cyclodextrin treatment induces segregation of Bad from rafts, which correlates with apoptosis. Our results suggest that the interaction of Bad with rafts is a dynamic process regulated by IL-4 and involved in the control of apoptosis.
authors.affiliation: Department of Immunology and Oncology, Centro Nacional de Biotecnología, Campus de Cantoblanco, Madrid, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Ayllón
authors.forename: Aarne
authors.initials: A
authors.lastname: Fleischer
authors.forename: Xavier
authors.initials: X
authors.lastname: Cayla
authors.forename: Alphonse
authors.initials: A
authors.lastname: García
authors.forename: Angelita
authors.initials: A
authors.lastname: Rebollo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology; D021962:Membrane Microdomains / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D051025:bcl-Associated Death Protein
chemical_list: C494820:Bad protein, mouse; D002352:Carrier Proteins; D051025:bcl-Associated Death Protein; D015847:Interleukin-4