title: Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing.
abstract: A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Ruslan
authors.initials: R
authors.lastname: Aphasizhev
authors.forename: Sandro
authors.initials: S
authors.lastname: Sbicego
authors.forename: Marian
authors.initials: M
authors.lastname: Peris
authors.forename: Sei-Heon
authors.initials: SH
authors.lastname: Jang
authors.forename: Inna
authors.initials: I
authors.lastname: Aphasizheva
authors.forename: Agda M
authors.initials: AM
authors.lastname: Simpson
authors.forename: Anatoly
authors.initials: A
authors.lastname: Rivlin
authors.forename: Larry
authors.initials: L
authors.lastname: Simpson
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D007891:Leishmania / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D008274:Magnesium / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012313:RNA / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing; D012316:RNA Nucleotidyltransferases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012313:RNA; D012316:RNA Nucleotidyltransferases; C031687:UTP-RNA uridylyltransferase; D008274:Magnesium