In vivo regulation of the mandibular organ in the edible crab, Cancer pagurus.
Considerable evidence indicates that methyl farnesoate (MF) production by the crustacean mandibular organs is negatively regulated by neuropeptides from the sinus gland (SG) in the eyestalk. In the crab Cancer pagurus, two neuropeptides (MO-IH-1 and -2) have been isolated from the SG that inhibit MF synthesis by mandibular organs of female crabs in vitro. To test their activity in vivo, we treated eyestalk-ablated male crabs with SG extracts (SGEs) or MO-IH-1 and -2. SGEs reduced haemolymph levels of MF by 60-80%, while MO-IH-1 and -2 had little effect. Protease treatment of SGEs destroyed the in vivo activity, suggesting that the extract contains an additional peptide responsible for the in vivo activity. When separated by reversed-phase high performance liquid chromatography (HPLC), the in vivo activity eluted in fractions prior to MO-IH-1 and -2. When mandibular organs were removed from animals previously treated in vivo with these active fractions, they had reduced levels of MF synthesis and activity of farnesoic acid O-methyl transferase compared with mandibular organs from animals treated with saline. Together, these results indicate that the regulation of the crustacean mandibular organ is complex and may involve several SG compounds. Some of these compounds (i.e., MO-IH-1 and -2) act directly on the tissue while others affect the mandibular organ indirectly.
Borst
D W
DW
Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120, USA. dwborst@ilstu.edu
Wainwright
G
G
Rees
H H
HH
D000818:Animals; D003386:Brachyura / Q000033:anatomy & histology* / Q000187:drug effects / Q000502:physiology*; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009479:Neuropeptides / Q000032:analysis / Q000494:pharmacology / Q000502:physiology*; D009928:Organ Specificity; D011863:Radioimmunoassay
D002457:Cell Extracts; D005231:Fatty Acids, Unsaturated; D009479:Neuropeptides; C100331:mandibular organ-inhibiting hormone 1; C100332:mandibular organ-inhibiting hormone 2; C032429:methyl farnesoate