title: Histologic evaluation of the efficacy of rhBMP-2 compared with autograft bone in sheep spinal anterior interbody fusion.
abstract: METHODS
  The sheep anterior lumbar spinal fusion model was used to study the efficacy of recombinant human bone morphogenetic protein-2 (rhBMP-2)-collagen composite in comparison with autograft to enhance spinal interbody fusion. Comparisons were drawn from temporal radiographic and end-point biomechanical and histologic data.
  
  
  OBJECTIVE
  To analyze histologically the ability of rhBMP-2 to achieve complete arthrodesis between vertebral bodies.
  
  
  BACKGROUND
  Studies using rhBMP for enhancement of anterior interbody fusion have used numerous endpoints. However, systematic histologic evaluation of the fusion has not been conducted.
  
  
  METHODS
  Twelve sheep underwent single-level anterior lumbar interbody fusion performed with a cylindrical fenestrated titanium interbody fusion device (INTER FIX, Medtronic Sofamor Danek, Inc., Memphis, TN). The device was filled either with rhBMP-2-collagen (n = 6) or autogenous iliac crest bone graft (n = 6). Radiologic evaluation was carried out at 2-month intervals, and all sheep were killed 6 months after surgery. Nondestructive biomechanical testing for stiffness to flexion, extension, and lateral bending moments, un-decalcified histology, and qualitative and quantitative histologic evaluation were performed.
  
  
  RESULTS
  Radiographs revealed a bony bridge anterior to the cage in five of six rhBMP-2-treated animals, whereas it was present only in one of five in the autogenous bone graft group. Segments treated with rhBMP-2 were 20% stiffer in flexion than autograft-treated segments at 6 months. Six of six in the rhBMP-2 group and two of six in the autograft group showed complete fusion. There was a significantly higher rate of bony continuity observed at the fenestrations of the rhBMP-2 group. Three times more number of cage fenestrations in the rhBMP-2 group demonstrated "all-bone" when compared with the autograft group (P < 0.001). Further, the scar tissue in and around the autograft-treated cages was 16-fold more (P < 0.01) than that seen for rhBMP-2-treated cages.
  
  
  CONCLUSIONS
  The study demonstrates that rhBMP-2 can lead to earlier radiologic fusion and a more consistent increased stiffness of the segments when compared with autograft in sheep anterior lumbar interbody fusion. Furthermore, a three times higher histologic fusion rate is attainable with significantly reduced fibrous tissue around the implant when rhBMP-2 is used.
authors.affiliation: Department of Orthopaedic Surgery and UCLA Comprehensive Spine Center, UCLA School of Medicine, Los Angeles, California, USA.
authors.forename: Harvinder S
authors.initials: HS
authors.lastname: Sandhu
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Toth
authors.forename: Ashish D
authors.initials: AD
authors.lastname: Diwan
authors.forename: H B
authors.initials: HB
authors.lastname: Seim
authors.forename: Linda E A
authors.initials: LE
authors.lastname: Kanim
authors.forename: J Michael
authors.initials: JM
authors.lastname: Kabo
authors.forename: A Simon
authors.initials: AS
authors.lastname: Turner
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D016025:Bone Transplantation / Q000379:methods*; D005260:Female; D005355:Fibrosis / Q000517:prevention & control; D006801:Humans; D007085:Ilium / Q000637:transplantation; D020007:Implants, Experimental; D008159:Lumbar Vertebrae / Q000166:cytology / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000601:surgery; D008161:Lumbosacral Region; D023421:Models, Animal; D011859:Radiography; D016059:Range of Motion, Articular / Q000187:drug effects; D011994:Recombinant Proteins; D012756:Sheep; D013123:Spinal Fusion / Q000009:adverse effects / Q000379:methods*; D014025:Titanium; D016212:Transforming Growth Factor beta; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D014025:Titanium