title: Ursolic acid of Origanum majorana L. reduces Abeta-induced oxidative injury.
abstract: Amyloid beta protein (Abeta) increases free radical production and lipid peroxidation in PC12 nerve cells, leading to apoptosis and cell death. The effect of ursolic acid from Origanum majorana L. on Abeta-induced neurotoxicity was investigated using PC12 cells. Pretreatment with isolated ursolic acid and vitamin E prevented the PC12 cell from reactive oxygen species (ROS) toxicity that is mediated by Abeta. The ursolic acid resulted in decreased Abeta toxicity assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and trypan blue assay. Thus, treatment with these antioxidants inhibited the Abeta-induced neurotoxic effect. Therefore, these results indicate that micromolar Abeta-induced oxidative cell death is reduced by ursolic acid from Origanum majorana L.
authors.affiliation: Graduate School of Biotechnology, Korea University, Seoul.
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Heo
authors.forename: Hong-Yon
authors.initials: HY
authors.lastname: Cho
authors.forename: Bumshik
authors.initials: B
authors.lastname: Hong
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Tae-Ryeon
authors.initials: TR
authors.lastname: Heo
authors.forename: Eun-Ki
authors.initials: EK
authors.lastname: Kim
authors.forename: Sung-Koo
authors.initials: SK
authors.lastname: Kim
authors.forename: Chang-Ju
authors.initials: CJ
authors.lastname: Kim
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology; D000097245:Ursolic Acid
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D017382:Reactive Oxygen Species; D014315:Triterpenes; D014810:Vitamin E; D000097245:Ursolic Acid