Genetic contribution of the interleukin-10 promoter polymorphism in endometriosis susceptibility.
OBJECTIVE
Interleukin-10 (IL-10) is an important immunomodulatory cytokine. The aim of this study was to investigate whether polymorphisms of the IL-10 gene promoter polymorphisms may be responsible in part for genetic susceptibility to endometriosis.


METHODS
Polymorphisms at position -1082 and -592 in the IL-10 promoter region were determined in 196 patients with endometriosis and 160 fertile healthy women by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP).


RESULTS
There were no statistically significant differences in the genotype and allele frequencies of IL-10 promoter polymorphism between the endometriosis and control groups. However, when subgrouped according to clinical features, the frequencies of the -592*CC genotype and -592*C allele were significantly increased in patients with autoantibodies to carbonic anhydrase II (anti-CA II ab) compared with controls.


CONCLUSIONS
IL-10 promoter polymorphisms were associated with the production of anti-CA II ab in patients with endometriosis, suggesting a role in the genetic susceptibility for endometriosis.
Kitawaki
Jo
J
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan. kitawaki@koto.kpu-m.ac.jp
Obayashi
Hiroshi
H
Ohta
Mitsuhiro
M
Kado
Noriko
N
Ishihara
Hiroaki
H
Koshiba
Hisato
H
Kusuki
Izumi
I
Tsukamoto
Katsumi
K
Hasegawa
Goji
G
Nakamura
Naoto
N
Yoshikawa
Toshikazu
T
Honjo
Hideo
H
D000328:Adult; D000483:Alleles; D001323:Autoantibodies / Q000097:blood; D024402:Carbonic Anhydrase II / Q000276:immunology; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic
D001323:Autoantibodies; D016753:Interleukin-10; D024402:Carbonic Anhydrase II