[Preparations of extracellular proteinases from Aspergillus ochraceus 513 and Aspergillus alliaceus 7dN1].
Preparations of extracellular proteolytic enzymes with high anticoagulant activity resembling protein C activators were isolated from the culture liquids of Aspergillus ochraceus 513 and Aspergillus alliaceus 7 dN1 by precipitation with ammonium sulfate and subsequent purification from ammonium ions by gel filtration on a column with Sephadex G-25. The pH and temperature activity optima and stability of the proteolytic enzymes from A. ochraceus 513 and A. alliaceus 7 dN1 were determined.
Batomunkueva
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BP
Microbiology Department, Biological Faculty, Moscow State University, Moscow, 119899 Russia.
Egorov
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NS
D000645:Ammonium Sulfate; D000925:Anticoagulants / Q000302:isolation & purification*; D001230:Aspergillus / Q000737:chemistry / Q000201:enzymology*; D001235:Aspergillus ochraceus / Q000201:enzymology; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D003911:Dextrans; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D011486:Protein C / Q000378:metabolism; D013696:Temperature
D000925:Anticoagulants; D003911:Dextrans; D011486:Protein C; C025614:sephadex; D010450:Endopeptidases; D000645:Ammonium Sulfate