Analytical method for monitoring concentrations of cyclosporin and lovastatin in vitro in an everted rat intestinal sac absorption model.
Cyclosporin A (CSA) and lovastatin (LV) are lipophilic drugs, which show poor and erratic absorption when administered perorally. The permeability of these compounds can be increased transiently by altering the membrane characteristics of the absorptive epithelium by the use of sorption promoters (SPs). In the present work a simple validated HPLC method utilizing an isocratic mobile phase with short retention times for CSA and LV was developed in order to monitor their concentrations in Kreb's Ringer bicarbonate (KRB) solution in vitro in intestinal sac absorption model. The same method was utilized to determine the apparent permeability coefficients and absorption profiles of CSA and LV by a modified Wilson-Wiseman method. Drugs were analysed by a reversed-phase HPLC method using a Shim-pack C18 column. An isocratic mobile phase containing acetonitrile and water in the proportions 70:30 and 80:20 was used for the HPLC analysis of CSA and LV, respectively. The flow-rate was 2 ml/min and quantitative determinations were carried out at 215 nm at 70 degrees C for CSA. In the case of LV the flow-rate was 1 ml/min and detection was done at 238 nm at 25 degrees C. The method was found to be specific as none of the proposed SPs, components of KRB or intestinal sac artefacts interfered with the drug peaks. Recovery studies and intra- and inter-day variations were within statistical limits. The limits of detection were 250 and 10 ng/ml and the limits of quantitation were 400 and 30 ng/ml for CSA and LV, respectively. The calibration curve was found to be linear in concentration range of 0.5-6 microg/ml for CSA and 0.05-0.4 microg/ml for LV. The proposed method was found to be rapid and selective and hence can be applied for continuous monitoring of CSA and LV in vitro in intestinal sac absorption studies.
Sharma
Pradeep
P
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Punjab, India.
Chawla
HarmanderPalSingh
H
Panchagnula
Ramesh
R
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000378:metabolism* / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D008148:Lovastatin / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D016572:Cyclosporine; D008148:Lovastatin