Blocking transforming growth factor-beta receptor signaling down-regulates transforming growth factor-beta1 autoproduction in keloid fibroblasts.
OBJECTIVE
To study transforming growth factor-beta1 (TGF-beta1) autoproduction in keloid fibroblasts and the regulation effect of blocking TGF-beta intracellular signaling on rhTGF-beta1 autoproduction.


METHODS
Keloid fibroblasts cultured in vitro were treated with either rhTGF-beta1 (5 ng/ml) or recombinant adenovirus containing a truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects of regulating gene expression of TGF-beta1 and its receptor I and II were observed with Northern blot.


RESULTS
rhTGF-beta1 up-regulated the gene expression of TGF-beta1 and receptor I, but not receptor II. Over-expression of the truncated receptor II down-regulated the gene expression of TGF-beta1 and its receptor I, but not receptor II.


CONCLUSIONS
TGF-beta1 autoproduction was observed in keloid fibroblasts. Over-expression of the truncated TGFbeta receptor II decreased TGF-beta1 autoproduction via blocking TGF-beta receptor signaling.
Liu
Wei
W
Department of Plastic and Reconstructive Surgery, Shanghai Institute of Plastic and Reconstructive Surgery, Tissue Engineering Center, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai 200011, China. liuwei-2000@yahoo.com
Cai
Zehao
Z
Wang
Danru
D
Wu
Xiaoli
X
Cui
Lei
L
Shang
Qingxin
Q
Qian
Yunliang
Y
Cao
Yilin
Y
D030201:Activin Receptors, Type I / Q000096:biosynthesis* / Q000494:pharmacology*; D002478:Cells, Cultured; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis* / Q000378:metabolism*; D012680:Sensitivity and Specificity; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D015854:Up-Regulation
D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D015534:Trans-Activators; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I