A study of the conditions of the supercritical fluid extraction in the analysis of selected anti-inflammatory drugs in plasma.
Supercritical fluid extraction (SFE) was employed to analyze selected anti-inflammatory drugs in plasma. Evaluation of selected drugs (ibuprofen, indomethacin, and flufenamic acid) was performed using the HPLC method on columns with the reverse phase C-18 and detection in the UV region of the spectrum. A study of the conditions of SFE carried out for 30 min at 50 degrees C investigated the magnitude of the pressure of carbon dioxide suitable for drug extraction, the selection of the collecting solvent, and the modification of CO2 with an organic solvent. The results of the study made it possible to determine the optimal procedure for SFE of ibuprofen, indomethacin, and flufenamic acid from plasma, which renders their HPLC quantification possible.
Klime
Jiri
J
Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic. klimes@faf.cuni.cz
Sochor
Jaroslav
J
Kríz
Josef
J
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D005439:Flufenamic Acid / Q000097:blood* / Q000737:chemistry; D007052:Ibuprofen / Q000097:blood* / Q000737:chemistry; D007213:Indomethacin / Q000097:blood* / Q000737:chemistry; D000432:Methanol; D008752:Methylene Chloride; D012997:Solvents
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012997:Solvents; D002245:Carbon Dioxide; D008752:Methylene Chloride; D005439:Flufenamic Acid; D007052:Ibuprofen; D007213:Indomethacin; D000432:Methanol