Induction of apoptotic pathway in the vestibule of cisplatin (CDDP)-treated guinea pigs.
BACKGROUND
During the process of apoptosis, double-stranded DNA is broken into single-stranded DNA by the action of caspases and caspase-activated deoxyribonuclease. We immunohistochemically examined the apoptotic changes induced by cisplatin in the vestibule of guinea pigs.


METHODS
Cisplatin (10 mg/kg b.w.) was intraperitoneally injected into guinea pigs and, 3 days after the injection, the animals were sacrificed by intracardiac perfusion of fixative. The temporal bones were then removed and immunohistochemically stained for caspase-activated deoxyribonuclease or caspase 3.


RESULTS
Both caspase-activated deoxyribonuclease and caspase 3 were observed in the dark cell area, transitional area and the sensory epithelium.


CONCLUSIONS
These findings suggest that apoptosis is involved in the vestibular dysfunction of the CDDP-treated patients.
Watanabe
K
K
Department of Oto-rhino-laryngology, Nippon Medical School, Tokyo, Japan. bxp02646@nifty.ne.jp
Jinnouchi
K
K
Baba
S
S
Yagi
T
T
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis; D002945:Cisplatin / Q000494:pharmacology*; D003851:Deoxyribonucleases / Q000096:biosynthesis; D006168:Guinea Pigs; D006801:Humans; D007150:Immunohistochemistry; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000906:Antibodies; D000970:Antineoplastic Agents; D003851:Deoxyribonucleases; C109663:caspase-activated deoxyribonuclease; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D002945:Cisplatin