Comparison of the biocompatibility of phosphate-buffered saline alone, phosphate-buffered saline supplemented with glucose, and dianeal 3.86%.
OBJECTIVE
We compared the effects of intraperitoneal infusion of phosphate-buffered saline (PBS, pH 7.4), of PBS supplemented with 3.86% glucose (G), and of standard dialysis solution [Dianeal 3.86%: Baxter Healthcare Corporation, Deerfield, IL, U.S.A. (D)] on intraperitoneal inflammation in dialyzed rats.


METHODS
After catheter implantation, rats were infused on day 1 with PBS, on day 3 with PBS+G, on day 5 with D, and on day 7 again with PBS (PBS-2). After a 4-hour dwell, dialysate samples were collected and analyzed.


RESULTS
All dialysate parameters studied [dialysate cell count, neutrophil:macrophage ratio (Ne:Ma), and total protein], except tumor necrosis factor alpha (TNFalpha), were comparable during both PBS infusions. During dialysis with PBS+G, the inflammatory response was suppressed as compared with the first dialysis with PBS (cell count, p < 0.001; Ne:Ma, p < 0.05; TNFalpha, p < 0.001; total protein, p < 0.001). During dialysis with D, peritoneal inflammatory parameters were further suppressed (cell count, p < 0.001 vs PBS and p < 0.01 vs PBS+G; Ne:Ma, p < 0.001 vs PBS and p < 0.05 vs PBS+G; TNFalpha, p < 0.001 vs PBS and p < 0.001 vs PBS+G; total protein, p < 0.001 vs PBS and p < 0.01 vs PBS+G).


CONCLUSIONS
Hypertonicity of the dialysis fluid suppresses intraperitoneal inflammatory parameters in rats. The suppression was even more severe when Dianeal 3.86% was used. That finding could be due to the low pH and presence of GDPs in the fluid.
Wieczorowska-Tobis
K
K
Department of Pathophysiology, University of Medical Sciences, Poznan, Poland. tobis@usoms.poznan.pl
Styszynski
A
A
Breborowicz
A
A
Oreopoulos
D G
DG
D000818:Animals; D001672:Biocompatible Materials; D002021:Buffers; D002452:Cell Count; D015314:Dialysis Solutions / Q000737:chemistry*; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D008264:Macrophages / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000473:pathology; D010710:Phosphates; D051381:Rats; D017208:Rats, Wistar; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride
D001672:Biocompatible Materials; D002021:Buffers; D015314:Dialysis Solutions; D010710:Phosphates; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride; D005947:Glucose