Comparison of high throughput screening technologies for luminescence cell-based reporter screens.
As higher density formats become more and more common in HTS labs, the expectations for maintaining faster, lower cost screens puts great pressure on traditional 96-well screens. In some cases higher density formats are not compatible with the assay. This seems especially true in cell-based assays. In our case, the nature of the cells' response forced us to remain in 96-well plates. In this paper, we describe the development of a luminescence reporter assay and its performance in two detection modes, flash and glow. The advantages in cost and throughput for each technique are explored, along with automation considerations. An additional new technology, the use of pins for low-volume transfers, is also briefly described because of its dramatic effect on our screen's throughput. However, it will be more thoroughly presented in a future publication. Comparing the technologies available for HTS aids in designing automated systems that meet the unique needs of each assay.
Davis
Gregory F
GF
The Procter and Gamble Company, Health Care Research Center, Mason, OH 45040-9462, USA. davis.gf@pg.com
Downs
Thomas R
TR
Farmer
Julie A
JA
Pierson
C Renee
CR
Roesgen
Jeff T
JT
Cabrera
Edelberto J
EJ
Nelson
Sandra L
SL
D019380:Anti-HIV Agents / Q000494:pharmacology; D001331:Automation; D001483:Base Sequence; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000037:antagonists & inhibitors; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013696:Temperature; D013876:Thiophenes / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection; D014471:Umbilical Veins / Q000166:cytology
D019380:Anti-HIV Agents; D007375:Interleukin-1; C099152:PD 121871; D013876:Thiophenes; D018799:Intercellular Adhesion Molecule-1