Toxicological effects of dietary arsenic exposure in lake whitefish (Coregonus clupeaformis).
Adult lake whitefish were fed As contaminated diets at nominal concentrations of 0, 1, 10, and 100 microg As/g food (dry weight) for 10, 30, and 64 days. Reduced feed consumption was observed in lake whitefish fed the 100 microg As/g food, beginning on day 45 of exposure. The accumulation and distribution of As in these fish are described in the previous manuscript [Pedlar, R.M., Klaverkamp, J.F., 2001. The accumulation and distribution of dietary arsenic in lake whitefish (Coregonus clupeaformis). Aquat. Toxicol., in press]. At the molecular level of organization, metallothionein (MT) induction occurred in lake whitefish fed the 100 microg As/g food after 10 and 30 days, and in fish fed the 1 and 10 microg As/g diets for 64 days. Dietary As exposure did not have a significant effect on plasma lipid peroxide (LPO) concentrations. At the tissue and organ level, mean liver somatic index decreased significantly in lake whitefish fed the 100 microg As/g food for 64 days. Blood parameters (hematocrit, hemoglobin concentration, red blood cell count, mean cell volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration) were not affected by exposure to As contaminated diets. Liver and gallbladder histopathologies were observed in lake whitefish fed all As contaminated diets after each duration of exposure. Histopathology observed in liver included nuclear, architectural and structural alterations, areas of inflammation, and focal necrosis. Sloughing of the epithelium, dilation of vascular elements, inflammation, edema, fibrosis, and increased width of the submucosa were some of the alterations observed in gallbladders of lake whitefish fed As contaminated diets. Both organs were sensitive to As exposure, as damage occurred with exposure to dietary concentrations of As as low as 1 microg/g. Whole organism parameters were unaltered by dietary As exposure. Based on the results of this study, histopathological alterations in liver and gallbladder, and hepatic MT induction may be useful indicators of As toxicity in environmental monitoring programs that also measure As concentrations in those tissues.
Pedlar
R M
RM
Department of Zoology, University of Manitoba, Winnipeg, MB, Canada R3T 2N2.
Ptashynski
M D
MD
Evans
R
R
Klaverkamp
J F
JF
D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D001151:Arsenic / Q000009:adverse effects* / Q000493:pharmacokinetics; D002455:Cell Division / Q000187:drug effects; D004781:Environmental Exposure; D005355:Fibrosis; D005704:Gallbladder / Q000187:drug effects / Q000473:pathology*; D007249:Inflammation; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008668:Metallothionein / Q000096:biosynthesis*; D012487:Salmonidae / Q000502:physiology*; D014018:Tissue Distribution; D014873:Water Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics
D014873:Water Pollutants; D008668:Metallothionein; D001151:Arsenic