Comparative toxinological and immunological studies on the nematocyst venoms of the Red Sea fire corals Millepora dichotoma and M. platyphylla.
A method appropriate for isolating of fire coral nematocysts of Millepora dichotoma (Md) and Millepora platyphylla (Mp) was described and compared with techniques that had been used before. Isolated nematocyst venoms of Md (Md-TX) and Mp (Mp-TX) were lethal to mice (had LD50 values of 0.51 and 0.21 microg/g mouse body, respectively) and displayed variable hemolytic, vasopermeable and dermonecrotic properties. The potent hemolysins of Md-TX and Mp-TX, which purified by gel filtration chromatography, possessed prominent proteins of molecular weights 35 and 31 kDa and had LD50 values 0.35 and 0.25 microg/g mouse, respectively. Hemolytic activities of crude venoms and their fraction could be inactivated using known anti-hemolytic agents. Both Md-TX and Mp-TX had distinguishable antigenic properties and their antisera raised in immunized mice and stung human were cross-reactive. ELISA assays showed an antigenic similarity among the studied fire coral homologous cytolytic counterparts.
Radwan
Faisal F Y
FF
Zoology Department, Faculty of Science at Sohag, South Valley University, Sohag, Egypt. faisal@pbrc.hawaii.edu
D000818:Animals; D000906:Antibodies / Q000097:blood; D001681:Biological Assay; D002199:Capillary Permeability / Q000187:drug effects; D002458:Cell Fractionation / Q000379:methods; D002850:Chromatography, Gel; D003063:Cnidaria / Q000737:chemistry* / Q000166:cytology; D003064:Cnidarian Venoms / Q000276:immunology* / Q000302:isolation & purification / Q000633:toxicity*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood; D019156:Indian Ocean; D007928:Lethal Dose 50; D051379:Mice; D009336:Necrosis; D015388:Organelles / Q000737:chemistry*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013347:Subcellular Fractions / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D003064:Cnidarian Venoms; D007074:Immunoglobulin G