Cofactors in sarcosine oxidase from Corynebacterium sp. U-96.
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme that was reported to contain 1 mol of covalently bound FAD and 1 mol of non-covalently-bound FAD. This work describes the result of reinvestigation of the cofactors in this enzyme. The enzyme was found to contain 1 mol of non-covalently-bound NAD+, 1 mol of non-covalently-bound FAD, and 1 mol of covalent FMN. The covalent FMN was identified by the mass and amino acid sequence analyses of the flavin peptide.
Mukouyama
Etsuko B
EB
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan. mukoyama@jet.sci.kitasato-u.ac.jp
Ohsawa
Hiromichi
H
Suzuki
Haruo
H
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003352:Corynebacterium / Q000201:enzymology*; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005415:Flavins / Q000737:chemistry / Q000378:metabolism*; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D050879:Sarcosine Oxidase; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D005415:Flavins; D010446:Peptide Fragments; D005182:Flavin-Adenine Dinucleotide; D005486:Flavin Mononucleotide; D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase