Examination of adhesive determinants in three species of Lactobacillus isolated from chicken.
The microbial adhesion process includes passive forces; electrostatic interactions; hydrophobic, steric forces; lipoteichoic acids; and specific structures, such as external appendages (lectins) and (or) extracellular polymers. In a previous work, we showed that Lactobacillus animalis, L. fermentum, and L. fermentum ssp. cellobiosus had lectinlike proteic structures on their surfaces and high hydrophobicity values on the cell surface of L. fermentum ssp. cellobiosus. Here, we examined the presence of the bacterial forces or structures that could be involved in the interaction between bacteria and epithelial cells. Lactobacillus animalis and L. fermentum possessed a net negative surface charge, whereas L. fermentum ssp. cellobiosus showed similar affinity to both cationic and anionic exchange resins, aggregated in the presence of ammonium sulfate, and had high affinity (75.4%) to a hydrophobic matrix. Only L. animalis was shown to have ribitol teichoic acids in the cell wall. The amount of polysaccharides from cell walls varied between different strains, with L. fermentum ssp. cellobiosus having the highest concentration. Lectin extracts obtained from lactobacilli did not possess sugar residues, thereby demonstrating the proteic nature of the superficial surface structures of three strains. The lactic acid bacteria studied here showed different surface determinants, which could be involved in the interactions between these lactobacilli and intestinal epithelial cells.
Gusils
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Centro de Referencias para Lactobacilos (CERELA-CONICET), and Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Argentina.
Cuozzo
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Sesma
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González
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D000818:Animals; D001422:Bacterial Adhesion; D002473:Cell Wall / Q000737:chemistry; D002645:Chickens / Q000382:microbiology*; D004064:Digestive System / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D019936:Probiotics; D013278:Stomach, Avian / Q000382:microbiology; D013499:Surface Properties