Dynamics of cAMP/cGMP in patients under a stress state.
OBJECTIVE
To investigate the dynamics of plasma cAMP/cGMP in patients during cardiac surgery, and its relationship to traumatic stress.


METHODS
Sixteen patients, aged 19.31 years+/-10.4 years, who underwent an open heart operation with cardiopulmonary bypass (CPB) and hypothermia were served as subjects. The arterial plasma concentrations of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were measured by radioimmunoassay 2 hours before operation, after heparinization, 20 minutes following CPB, at the end of the operation, and 24 and 72 hours postoperatively, respectively. The patients' preoperative blood samples were heparinized and the venous blood samples of 30 healthy blood donors were taken to measure the levels of cAMP and cGMP as heparin and normal controls separately.


RESULTS
There were no statistical difference among the heparin control, preoperative level and normal control. The peak values of cAMP and cGMP occurred during CPB and plasma cAMP levels changed synchronously with intensities of operative stimulus to human body. However cGMP level was mainly related to the operative stimulus to the heart and CPB. The cAMP value was positively correlated with the cGMP value (r=0.6313, P<0.001).


CONCLUSIONS
Dynamic variation of plasma cyclic ribonucleotide can be considered as a reference parameter for intensity of traumatic stress.
Chen
Peng
P
The Second Affiliated Hospital, Medical School, Zhejiang University, Hangzhou 31 0009, China.
Chai
Ying
Y
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002315:Cardiopulmonary Bypass; D003327:Coronary Disease / Q000097:blood* / Q000601:surgery*; D000242:Cyclic AMP / Q000032:analysis / Q000097:blood*; D006152:Cyclic GMP / Q000032:analysis / Q000097:blood*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011184:Postoperative Period; D011300:Preoperative Care; D011863:Radioimmunoassay; D012016:Reference Values; D012680:Sensitivity and Specificity; D013315:Stress, Psychological / Q000097:blood*
D015415:Biomarkers; D000242:Cyclic AMP; D006152:Cyclic GMP