DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA-cleaving agents.
Monodisperse magnetic nanoparticles conjugated with complementary oligonucleotide sequences self-assemble into stable magnetic nanoassemblies resulting in a decrease of the spin-spin relaxation times (T2) of neighboring water protons. When these nanoassemblies are treated with a DNA cleaving agent, the nanoparticles become dispersed, switching the T2 of the solution back to original values. These qualities render the developed nanoparticles and their nanoassemblies as magnetic relaxation switches capable of screening for DNA-cleaving compounds by magnetic resonance methods such as MRI and NMR.
Perez
J Manuel
JM
MGH-Center for Molecular Imaging Research, Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA.
O'Loughin
Terence
T
Simeone
F Joseph
FJ
Weissleder
Ralph
R
Josephson
Lee
L
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis; D036103:Nanotechnology; D019906:Nuclear Magnetic Resonance, Biomolecular; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000378:metabolism; D014764:Viral Proteins
D009841:Oligonucleotides; D014764:Viral Proteins; D004247:DNA; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499296:dam protein, Bacteriophage T2; D015245:Deoxyribonuclease BamHI