The structure of an unusual leghemoglobin gene from soybean.
A clone containing an unusual leghemoglobin (Lb) gene was isolated from a soybean DNA library present in Charon 4A phage. DNA sequence analysis revealed that the isolated Lb gene has three intervening sequences (IVS-1, IVS-2 and IVS-3) located in the same positions as those found in other Lb genes. Due to a large increase of IVS-2 and IVS-3, the isolated Lb gene is about twice the size of a normal Lb gene. The coding sequence derived from the DNA sequence corresponds to no known soybean Lb and attempts to find a corresponding mRNA failed. In addition, the 5'-flanking sequence of the Lb gene is mutated in two regions which seem to be important for transcription. It is, therefore, tentatively suggested that the isolated Lb gene is non-functional, and consequently is an Lb pseudogene.
Wiborg
O
O
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Hyldig-Nielsen
J J
JJ
Jensen
E O
EO
Paludan
K
K
Marcker
K A
KA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000032:analysis; D017343:Genes, Plant; D007874:Leghemoglobin / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013025:Glycine max / Q000235:genetics*
D018744:DNA, Plant; D007874:Leghemoglobin