Application of capillary zone electrophoresis to study the properties of rhodanese from Acidithiobacillus ferrooxidans.
A new capillary zone electrophoretic method was applied to the assay of enzymic activity of rhodanese from Acidithiobacillus ferroxidans. The enzyme activity determined by capillary zone electrophoresis was compared with that determined by discontinuous spectrophotometry, the values obtained being in good agreement. The method was also used to evaluate Michaelis constants of cyanide and thiocyanate as substrates; a new approach was developed to solve the problem with variable ionic strength of the samples. The pH and temperature optima for the enzyme were also determined.
Bouchal
P
P
Department of Biochemistry, Faculty of Science, Masaryk University, 611 37 Brno, Czechia.
Glatz
Z
Z
Janiczek
O
O
Mandl
M
M
D001426:Bacterial Proteins / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D020560:Proteobacteria / Q000201:enzymology*; D015203:Reproducibility of Results; D013696:Temperature; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry*
D001426:Bacterial Proteins; D013884:Thiosulfate Sulfurtransferase