The role of SmpB protein in trans-translation.
The function of SmpB protein in the trans-translation system was evaluated using the well-defined cell-free translation system consisting of purified ribosome, alanyl-tRNA synthetase and elongation factors. The analysis showed that SmpB protein enhances alanine-accepting activity of tmRNA and that SmpB protein and tmRNA are sufficient to complete the trans-translation process in the presence of translational components. Moreover, SmpB is indispensable in the addition of tag-peptide onto ribosomes by tmRNA. In particular, the A-site binding of tmRNA is inhibited in the absence of SmpB.
Shimizu
Yoshihiro
Y
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, 277-8562, Chiba Prefecture, Japan.
Ueda
Takuya
T
D000409:Alanine / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis / Q000502:physiology*; D012329:RNA, Bacterial / Q000502:physiology; D016601:RNA-Binding Proteins / Q000502:physiology*
D012329:RNA, Bacterial; D016601:RNA-Binding Proteins; C430328:small protein B; C096222:tmRNA; D000409:Alanine