Nucleoprotein structure of immediate-early promoters Zp and Rp and of oriLyt of latent Epstein-Barr virus genomes.
Genomic footprints across Rp, Zp, and oriLyt of Epstein-Barr virus (EBV) have been conducted in a panel of latently infected B-cell lines. Close protein-base contacts were found about 360 nucleotides upstream of the Zp initiation site. Gel shifts and transient transfection assays indicated that an Sp1-NF1 locus may serve as a repressive transcriptional element against Zp induction from latent EBV genomes.
Niller
Hans Helmut
HH
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, D-93053 Regensburg, Germany. Hans-Helmut.Niller@klinik.uni-regensburg.de
Salamon
Daniel
D
Uhlig
Jörg
J
Ranf
Stefanie
S
Granz
Marcus
M
Schwarzmann
Fritz
F
Wolf
Hans
H
Minarovits
Janos
J
D001402:B-Lymphocytes; D001483:Base Sequence; D002460:Cell Line; D018983:DNA Footprinting; D016679:Genome, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D017735:Virus Latency
D017874:Immediate-Early Proteins; D009698:Nucleoproteins