title: Retinoic acid-inducible gene-I is induced in endothelial cells by LPS and regulates expression of COX-2.
abstract: Bacterial lipopolysaccharides (LPS) induce expression of multiple genes in endothelial cells, which are critical cellular effectors in various pathologic syndromes. Using subtractive hybridization to identify genes that are differentially induced in human endothelial cells treated with LPS, we found that retinoic acid-inducible gene I (RIG-I) is induced in endothelial cells stimulated with LPS. RIG-I encodes a protein belonging to the DExH-box family which has diverse roles in regulation of gene expression and cellular functions. Cyclooxygenase-2 (COX-2) is also induced in endothelial cells by LPS. Overexpression of RIG-I selectively upregulated expression of COX-2 and also induced COX-2 promoter activity. RIG-I is an inducible gene in stimulated endothelial cells that may have important roles in vascular pathology by virtue of its ability to regulate expression of the COX-2 gene product.
authors.affiliation: Department of Vascular Biology, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan. timaizum@cc.hirosaki-u.ac.jp
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Satoko
authors.initials: S
authors.lastname: Aratani
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakajima
authors.forename: Mary
authors.initials: M
authors.lastname: Carlson
authors.forename: Tomoh
authors.initials: T
authors.lastname: Matsumiya
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tanji
authors.forename: Keizou
authors.initials: K
authors.lastname: Ookawa
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Shigeki
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Thomas M
authors.initials: TM
authors.lastname: McIntyre
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Prescott
authors.forename: Guy A
authors.initials: GA
authors.lastname: Zimmerman
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008565:Membrane Proteins; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D020365:RNA Helicases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011971:Receptors, Immunologic; D015533:Transcriptional Activation; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D014212:Tretinoin; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases