High-temperature gas chromatography-mass spectrometry with glass capillary columns for the screening of natural products.
High-temperature high resolution gas chromatography (HT-HRGC) and HT-HRGC coupled to mass spectrometry (HT-HRGC-MS) are powerful but relatively unexplored tools for the analysis of crude extracts and fractions of natural products. To illustrate the scope of the technique the direct characterization of several compounds, present in crude extracts of leaves and stems of Croton hemiargyreus Muell. Arg. var. hemiargyreus was undertaken, without derivatization or clean-up procedures. Both practical aspects and limitations of HT-HRGC and HT-HRGC-MS were evaluated resulting in a simple, straightforward and extremely powerful technique for the analysis of complex mixtures.
dos
SantosPereirAlberto
S
LADETEC, Instituto de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, Brazil. ladetec@iq.ufrj.br
Carbonell
Silvia Albero
SA
de
AquinoNetoFranciscoRadler
A
do
AmaralAnaClaudiaFernandes
A
Barnes
Roderick A
RA
D001688:Biological Products / Q000032:analysis*; D029802:Croton / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D005898:Glass; D006358:Hot Temperature
D001688:Biological Products