A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells.
We describe the construction of a bovine papilloma virus-based vector (pCGBPV9) which contains a dominant selectable marker and replicates autonomously in both mouse and Escherichia coli cells. This vector contains the complete bovine papilloma virus genome, a ColE1 replication origin and a dominant selectable marker conferring resistance to kanamycin in bacteria and G418 in eukaryotic cells. A high number of G418R colonies are obtained after transfer of pCGBPV9 into mouse C127 cells. These G418R colonies contain vector DNA which replicates autonomously at approximately 10-30 copies per cell. The molecules are in most cases unrearranged and can be rescued into E. coli cells by bacterial transformation.
Matthias
P D
PD
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg, FRG.
Bernard
H U
HU
Scott
A
A
Brady
G
G
Hashimoto-Gotoh
T
T
Schütz
G
G
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D003360:Cosmids / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005799:Genes, Dominant; D005814:Genes, Viral; D005819:Genetic Markers; D005822:Genetic Vectors; D007613:Kanamycin Resistance / Q000235:genetics; D051379:Mice; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014162:Transfection; D014779:Virus Replication
D005819:Genetic Markers