[Cloning and characterization of D-113 gene promoter from cotton].
To study the expression of late embryogenesis abundant gene in seeds, the 1,024 bp 5' flanking sequence of D-113 gene, a late embryogenesis abundant gene of Gossypium hirsutum cv. Coker 312, was cloned by PCR. The similarity compared with the sequence of Lea protein gene family published was 92.50%. There are three putative ABREs and one enhancer-like which riches A/T in the promoter. The promoter was fused to the beta-glucuronidase gene to form pLD II. Via a particle bombardment, pLD II was introduced into embryogenic calli of cotton and seeds of Brassica napus which were all treated with abscisic acid for 3d before bombardment, also into roots, stems and leafs of cotton. Transient expression was measured histochemically as spot number 24 h after bombardment. GUS sexpression was observed in the seeds of Brassica napus and the embryogenic calli of cotton, but not found in roots and leaves of cotton. Those results indicated that the expression of D-113 gene promoter was embryo specific.
Luo
Ke-Ming
KM
Biotechnology Research Center, Southwest Agriculture University, Changqing Beipei 400716, China.
Guo
Yu-Long
YL
Xiao
Yue-Hua
YH
Hou
Lei
L
Pei
Yan
Y
D001483:Base Sequence; D019470:Biolistics / Q000379:methods; D029688:Brassica napus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D003368:Gossypium; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology; D014170:Transformation, Genetic
D018744:DNA, Plant; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant