Sensitization of renal transplant candidates by cryopreserved cadaveric venous or arterial allografts.
BACKGROUND
Cryopreserved cadaveric venous or arterial allografts are used in ESRD patients as an alternative to synthetic grafts for hemodialysis access. We evaluated the effect of these allografts on the PRA against HLA class I and II antigens in 11 ESRD patients awaiting a kidney transplant.


METHODS
Flow Cytometry using purified antigen coated beads (Flow PRA Beads) was used to determine PRA against HLA class I and II antigens.


RESULTS
Patients with no antibody prior to allograft implantation were all positive for HLA class I and II antibodies after implantation. Patients with anti-HLA class I and II antibodies prior to allografting, developed higher antibody titers. Of the 11 patients that received cryopreserved cadaveric allografts no deaths were reported. Two grafts were removed due to thrombosis consistent with rejection.


CONCLUSIONS
Recipients of cadaveric venous or arterial allografts elicit an HLA antibody response attesting to the antigenicity of cryopreserved cadaveric allografts.
Lopez-Cepero
Mayra
M
Immunology Laboratory, LifeLink Foundation 2 and LifeLink Transplant Institute 3, Tampa, FL 33606, USA.
Sanders
Charles E
CE
Buggs
Jacentha
J
Bowers
Victor
V
D000328:Adult; D001158:Arteries / Q000637:transplantation*; D002102:Cadaver; D015925:Cryopreservation; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D016030:Kidney Transplantation; D008297:Male; D014184:Transplantation, Homologous; D014680:Veins / Q000637:transplantation*
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies