title: Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts.
abstract: Skin biopsies were taken from female dogs, the primary hair follicles isolated and the dermal papilla dissected. After incubation in supplemented Amniomax complete C100 medium in 24-well culture plates, the dermal papilla cells (DPC) grew to confluence within 3 weeks. Thereafter, they were subcultivated every 7 days. Dermal fibroblast (DFB) cultures were established by explant culture of interfollicular dermis in serum-free medium, where they reached confluence in 10 days. They were subcultivated every 5 days. For immunohistochemistry, cells were grown on cover slips for 24 h, fixed and stained with antibodies against collagen IV and laminin. DPC showed an aggregative growth pattern and formation of pseudopapillae. Intensive staining for collagen IV and laminin could be observed until the sixth passage. DFB grew as branching, parallel lines and showed only weak staining for collagen IV and laminin.
authors.affiliation: Institute for Biochemistry, Ludwig Boltzmann Institute for Veterinary Endocrinology, Veterinary University of Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
authors.forename: Christine B
authors.initials: CB
authors.lastname: Bratka-Robia
authors.forename: Gerda
authors.initials: G
authors.lastname: Mitteregger
authors.forename: Amanda
authors.initials: A
authors.lastname: Aichinger
authors.forename: Monika
authors.initials: M
authors.lastname: Egerbacher
authors.forename: Magdalena
authors.initials: M
authors.lastname: Helmreich
authors.forename: Elmar
authors.initials: E
authors.lastname: Bamberg
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000662:veterinary; D003094:Collagen / Q000032:analysis; D004285:Dogs / Q000033:anatomy & histology*; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D018859:Hair Follicle / Q000166:cytology*; D012867:Skin / Q000166:cytology*
chemical_list: D003094:Collagen