title: Potent cytotoxic lignans from Justicia procumbens and their effects on nitric oxide and tumor necrosis factor-alpha production in mouse macrophages.
abstract: A new lignan glycoside, 4-O-alpha-L-arabinopyranosyl-(1' "-->2' ')-beta-D-apiofuranosyldiphyllin (2), named procumbenoside A, and 11 known compounds were isolated from the whole plant of Justicia procumbens. The structure of 2 was established by spectral analysis and chemical methods. The known compounds justicidin A (1), diphyllin (3), and tuberculatin (4) showed potent cytotoxic effects against a number of cancer cells in vitro. Compounds 1 and 4 also strongly enhanced tumor-necrosis factor-alpha (TNF-alpha) generation from mouse macrophage-like RAW 264.7 cells stimulated with lipopolysaccharide (LPS).
authors.affiliation: School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, Republic of China.
authors.forename: Shiow-Hwa
authors.initials: SH
authors.lastname: Day
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Lin
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Tsai
authors.forename: Lo-Ti
authors.initials: LT
authors.lastname: Tsao
authors.forename: Horng-Huey
authors.initials: HH
authors.lastname: Ko
authors.forename: Mei-Ing
authors.initials: MI
authors.lastname: Chung
authors.forename: Jeng-Chang
authors.initials: JC
authors.lastname: Lee
authors.forename: Jih-Pyang
authors.initials: JP
authors.lastname: Wang
authors.forename: Shen-Jeu
authors.initials: SJ
authors.lastname: Won
authors.forename: Chun-Nan
authors.initials: CN
authors.lastname: Lin
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D052117:Benzodioxoles; D002461:Cell Line, Transformed / Q000187:drug effects; D004148:Dioxolanes / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000032:analysis; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013624:Taiwan; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D052117:Benzodioxoles; D004148:Dioxolanes; D006027:Glycosides; D017705:Lignans; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C049719:justicidins; C455679:procumbenoside A; C103139:tuberculatin; D009569:Nitric Oxide; C010130:diphyllin