Treatment of streptozotocin-induced diabetes mellitus by transplantation of islet cells plus bone marrow cells via portal vein in rats.
BACKGROUND
We have established a new method for the transplantation of allogeneic pancreatic islets (PIs) using sublethal irradiation (9 Gy) plus simultaneous transplantation of PIs and bone marrow cells (BMCs) via the portal vein (PV) followed by intravenous (i.v.) injection of donor BMCs (9 Gy + PV + i.v.).


METHODS
Approximately 600 PIs of Brown Norway (BN: RT1An, RT1Bn) rats were transplanted into the liver of streptozotocin-induced diabetic Fischer 344 (F344: RT1Al, RT1Bl) rats via the PV. BMCs (3x108) of BN rats were injected via the PV or i.v. into the recipients simultaneously. In some groups, additional i.v. injections of BMCs from BN rats were given 5 days after the PI transplantation.


RESULTS
All the recipients (10 of 10) in the 9 Gy + PV + i.v. group showed normoglycemia for more than 1 year, whereas PIs were rejected within 30 days after transplantation in the group of 9 Gy + i.v. + i.v.


CONCLUSIONS
These results suggest that simultaneous transplantation of PIs and BMCs via the PV is effective in inducing persistent tolerance.
Ikebukuro
Kazuya
K
First Department of Pathology, Kansai Medical University, Osaka 570-8506, Japan.
Adachi
Yasushi
Y
Yamada
Yuichiro
Y
Fujimoto
Shimpei
S
Seino
Yutaka
Y
Oyaizu
Haruki
H
Hioki
Koshiro
K
Ikehara
Susumu
S
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000473:pathology; D006085:Graft Survival / Q000276:immunology / Q000502:physiology*; D007165:Immunosuppression Therapy / Q000379:methods; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000473:pathology / Q000502:physiology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D011169:Portal Vein; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D013602:T-Lymphocytes, Cytotoxic; D013997:Time Factors
D001786:Blood Glucose