title: Gene and protein expressions of type I collagen are regulated tissue-specifically in rat hyaline cartilages in vivo.
abstract: The present study was designed to investigate how rat hyaline cartilages at various sites in vivo express the gene and protein of type I collagen using in situ hybridization and immunohistochemistry. The gene of pro alpha 1(I) collagen was expressed by chondrocytes in articular cartilage, and the protein of type I collagen was identified in the cartilage matrix. In contrast, growth plate cartilage expressed the gene of pro alpha 1(I) collagen, but no protein of type I collagen. Neither gene nor protein of type I collagen was expressed in cartilages of trachea and nasal septum. The present study suggested that expression of type I collagen in hyaline cartilages may be regulated tissue-specifically at gene and/or protein levels.
authors.affiliation: Second Department of Oral Anatomy, Tohoku University School of Dentistry, Sendai, Japan. sasano@anat.dent.tohoku.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Sasano
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: J X
authors.initials: JX
authors.lastname: Zhu
authors.forename: H
authors.initials: H
authors.lastname: Ohtani
authors.forename: I
authors.initials: I
authors.lastname: Mizoguchi
authors.forename: M
authors.initials: M
authors.lastname: Kagayama
mesh_terms: D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D024042:Collagen Type I / Q000096:biosynthesis* / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D006818:Hyalin / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D012313:RNA / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D024042:Collagen Type I; D018076:DNA, Complementary; D012313:RNA