Fine scale mapping in the sex locus region of the honey bee (Apis mellifera).
Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. The construction of specific inbred crosses facilitates fine scale mapping in the sex locus region of the honey bee. The high recombination rate in the honey bee reduces the physical distance between markers compared with model organisms and facilitates a novel gene isolation strategy based on step-wise creation of new markers within small physical distances. We show that distances less than 25 kb can be efficiently mapped with a mapping population of only 1000 individuals. The procedure described here will accelerate the mapping, analysis and isolation of honey bee genes.
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Martin-Luther Universität Halle/Wittenberg, Institut für Zoologie, Molekulare Okologie, Biozentrum, Weinberg Weg 22, 06120 Halle, Germany. hasselmann@biozentrum.uni-halle.de
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D000818:Animals; D001516:Bees / Q000235:genetics*; D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D008297:Male