[Establishment of detective systems for GL-7ACA acylase expression].
Glutaryl-7-amino cephalosporanic acid (GL-7ACA) acylase catalyzes the conversion of GL-7ACA to 7-amino cephalosporanic acid (7-ACA). The product 7-ACA is a starting compound for semi-synthetic cephalosporin antibiotics in industry. In order to detect the expression and specific activity of protein-engineered GL-7ACA acylase accurately, two useful detective systems for its expression has been established, in which reporter genes xylE and lacZ were fused to the downstream the GL-7ACA acylase gene acy respectively and the activity of catechol dioxygenase or beta-galactosidase could indicate the amount of acy expression.
Li
X
X
Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
Zhang
W
W
Mao
X
X
Cai
X
X
Yang
Y L
YL
Zhao
G P
GP
Jiang
W H
WH
D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D004789:Enzyme Activation; D017930:Genes, Reporter / Q000235:genetics / Q000502:physiology; D005822:Genetic Vectors; D007763:Lac Operon / Q000502:physiology*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D010399:Penicillin Amidase / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods
D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D010399:Penicillin Amidase; C046586:glutarylamidocephalosporanic acid acylase