Differential surrogate light chain expression governs B-cell differentiation.
Surrogate light chain expression during B lineage differentiation was examined by using indicator fluorochrome-filled liposomes in an enhanced immunofluorescence assay. Pro-B cells bearing surrogate light chain components were found in mice, but not in humans. A limited subpopulation of relatively large pre-B cells in both species expressed pre-B cell receptors. These cells had reduced expression of the recombinase activating genes, RAG-1 and RAG-2. Their receptor-negative pre-B cell progeny were relatively small, expressed RAG-1 and RAG-2, and exhibited selective down-regulation of VpreB and lambda5 expression. Comparative analysis of the 2 pre-B cell subpopulations indicated that loss of the pre-B cell receptors from surrogate light chain gene silencing was linked with exit from the cell cycle and light chain gene rearrangement to achieve B-cell differentiation.
Wang
Yui-Hsi
YH
Department of Medicine, Division of Developmental and Clinical Immunology, University of Alabama at Birmingham, 1824 6th Avenue S, Birmingham, AL 35294-3300, USA.
Stephan
Robert P
RP
Scheffold
Alexander
A
Kunkel
Désirée
D
Karasuyama
Hajime
H
Radbruch
Andreas
A
Cooper
Max D
MD
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology; D020868:Gene Silencing; D016654:Genes, RAG-1 / Q000235:genetics; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D054444:Immunoglobulin Light Chains, Surrogate; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D009687:Nuclear Proteins; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D017931:DNA Primers; D004268:DNA-Binding Proteins; D007147:Immunoglobulin Light Chains; D054444:Immunoglobulin Light Chains, Surrogate; D007146:Immunoglobulin lambda-Chains; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; D011947:Receptors, Antigen, B-Cell; C064655:V(D)J recombination activating protein 2