title: Comparison of SSI with APCI as an interface of HPLC-mass spectrometry for analysis of a drug and its metabolites.
abstract: Sonic spray ionization (SSI) was compared with atmospheric pressure chemical ionization (APCI) as an interface of high-performance liquid chromatography (HPLC)-mass spectrometry (MS) for sensitive analyses of a neuroleptic drug, haloperidol and its two metabolites, such as reduced haloperidol and 4-(4-chlorophenyl)-4-hydroxypiperidine (CPHP), in biological samples. For both SSI and APCI interfaces, HPLC-MS-MS gave higher sensitivity than HPLC-MS. The sensitivities by HPLC-SSI-MS-MS for haloperidol and reduced haloperidol were 100 and 30 times higher, respectively, than those by HPLC-APCI-MS-MS; no spectrum with recognizable peaks was obtained for CPHP with the APCI interface. Therefore, detection limits and regression equations were examined by the HPLC-SSI-MS-MS for human plasma and urine samples spiked with the above drug and its metabolites. Haloperidol, reduced haloperidol, and CPHP showed good linearity in the ranges of 5-800, 10-800, and 100-800 ng/mL, respectively, for both human plasma and urine; their detection limits were 2.5, 5, and 75 ng/mL, respectively, using a new polymer HPLC column which enabled direct application of biological samples.
authors.affiliation: Department of Legal Medicine, Aichi Medical University School of Medicine, Japan. arinobu1@hotmail.com
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Arinobu
authors.forename: Hideki
authors.initials: H
authors.lastname: Hattori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Seno
authors.forename: Akira
authors.initials: A
authors.lastname: Ishii
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis / Q000097:blood / Q000652:urine; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006220:Haloperidol / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010880:Piperidines / Q000032:analysis / Q000097:blood / Q000652:urine; D014465:Ultrasonics
chemical_list: D014150:Antipsychotic Agents; D004364:Pharmaceutical Preparations; D010880:Piperidines; C040692:4-(4-(4-chlorophenyl)-4-hydroxy-1-piperidinyl)-1-(4-fluorophenyl)-1-butanol; C074930:4-(4'-chlorophenyl)-4-piperidinol; D006220:Haloperidol