[Successful direct transfer of a deep frozen-thawed equine embryo].
Embryos were flushed on day 7 after ovulation from two mares, and frozen using a conventional slow freezing procedure in phosphate buffered (PBS) saline supplemented with 10% FCS, 1.5 mol/L ethylene glycol and 0.25 mol/L sucrose. One of the two embryos was thawed after 10 months of storage in liquid nitrogen and transferred directly (without dilution of the cryoprotectant and quality examination) to a synchronized recipient. This transfer resulted in the birth of a live female foal. To our knowledge, this is the first live foal born after direct transfer of a frozen-thawed equine embryo.
Ulrich
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Interuniversitäres Forschungsinstitut für Agrarbiotechnologie, Tulln, Osterreich. MNowshari@hotmail.com
Nowshari
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MA
D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D006736:Horses / Q000502:physiology*; D011247:Pregnancy