Rapid monitoring of recombinant protein products: a comparison of current technologies.
Specific measurement of recombinant protein titer in a complex environment during industrial bioprocessing has traditionally relied on labor-intensive and time-consuming immunoassays. In recent years, however, developments in analytical technology have resulted in improved methods for protein product monitoring during bioprocessing. The choice of product-monitoring technology for a particular bioprocess will depend on a variety of assay factors and instrument-specific factors. In this article, we have compiled an overview of the advantages and disadvantages of the most commonly used technologies used: electrochemiluminescence, optical biosensors, rapid chromatography and nephelometry. The advantages of each technology for measuring both small and large recombinant therapeutic proteins are compared with a conventional enzyme-linked immunosorbent assay (ELISA) technique.
Baker
Kym N
KN
Research School of Biosciences, University of Kent, Canterbury, UK.
Rendall
Mark H
MH
Patel
Ashvin
A
Boyd
Paul
P
Hoare
Mike
M
Freedman
Robert B
RB
James
David C
DC
D015374:Biosensing Techniques / Q000191:economics / Q000379:methods / Q000639:trends; D004563:Electrochemistry / Q000191:economics / Q000295:instrumentation / Q000639:trends; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods / Q000639:trends; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D009391:Nephelometry and Turbidimetry / Q000191:economics / Q000379:methods / Q000639:trends; D055095:Optics and Photonics / Q000295:instrumentation; D011994:Recombinant Proteins / Q000032:analysis*
D011994:Recombinant Proteins