Studies of the ceruloplasmin-lactoferrin complex.
We have previously shown that iron-containing human lactoferrin (LF) purified from breast milk is able to form both in vitro and in vivo a complex with ceruloplasmin (CP), the copper-containing protein of human plasma. Here we present evidence that the CP-LF complex is dissociated by high concentrations of NaCl, CaCl2, or EDTA, or by decreasing the pH to 4.7. In addition, DNA, bacterial lipopolysaccharide, and heparin can displace CP from its complex with LF. Antibodies to either of the two proteins also cause dissociation of the complex.
Pulina
Maria O
MO
Institute for Experimental Medicine, Saint-Petersburg, Russia. molgen.@molgen.iem.ras.spb.ru
Zakharova
Elena T
ET
Sokolov
Alexei V
AV
Shavlovski
Mikhail M
MM
Bass
Mikhail G
MG
Solovyov
Kirill V
KV
Kokryakov
Vladimir N
VN
Vasilyev
Vadim B
VB
D002122:Calcium Chloride / Q000494:pharmacology; D002570:Ceruloplasmin / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000378:metabolism*; D046911:Macromolecular Substances; D008895:Milk, Human / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology
D046911:Macromolecular Substances; D012965:Sodium Chloride; D004492:Edetic Acid; D002570:Ceruloplasmin; D007781:Lactoferrin; D002122:Calcium Chloride