title: Gas sensor arrays for early detection of infection in mammalian cell culture.
abstract: The detection of bacterial infections in a mammalian cell culture process is realised using a gas sensor array. In production-scale and laboratory-scale cultivations of a perfused recombinant CHO-cell culture producing human blood coagulation Factor VIII, we show that the gas sensor array identifies bacterial contamination earlier than conventional methods. The sensitivity of the instrument is verified by inoculation of a blank cell culture medium with defined bacterial cell counts.
authors.affiliation: Department of Physics and Measurement Technology, Division of Biotechnology, Swedish Sensor Centre and Division of Biotechnology, Linköping University, S-581 83 Linköping, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bachinger
authors.forename: Ulrich
authors.initials: U
authors.lastname: Riese
authors.forename: Rolf K
authors.initials: RK
authors.lastname: Eriksson
authors.forename: Carl Fredrik
authors.initials: CF
authors.lastname: Mandenius
mesh_terms: D000818:Animals; D001409:Bacillus cereus / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D019149:Bioreactors; D015374:Biosensing Techniques; D016466:CHO Cells; D015169:Colony Count, Microbial; D006224:Cricetinae; D003470:Culture Media; D004340:Drug Contamination; D005169:Factor VIII / Q000096:biosynthesis; D005740:Gases / Q000032:analysis; D006801:Humans; D016982:Micrococcus luteus / Q000302:isolation & purification; D009862:Online Systems; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D005740:Gases; D011994:Recombinant Proteins; D005169:Factor VIII