Plastid state- and light-dependent regulation of the expression of nucleus-encoded genes for chloroplast proteins in the flagellate Euglena gracilis.
Interorganellar regulatory interactions in the flagellate Euglena gracilis were shown to be more complicated than in green algae and higher plants. Euglena plastids have a much more complex influence on nuclear gene expression than was previously thought. The petJ gene for cytochrome c6 represents a group of nucleus-encoded genes for chloroplast proteins, the expression of which is influenced by the state of plastids at the transcriptional level. Moroever, the regulation of these genes might be light-dependent. In contrast, for nucleus-encoded small subunit of ribulose-bisphosphate carboxylase, chlorophyll a/b binding protein, and porphobilinogen deaminase transcript levels are unchanged in wild-type cells relative to white mutants. In these cases there is no plastid-derived signal operative during transcription. Porphobilinogen deaminase appeared to be regulated even at the post-translational level.
Vacula
R
R
Institute of Cell Biology, Comenius University, 811 07 Bratislava, Slovakia.
Steiner
J M
JM
Krajcovic
J
J
Ebringer
L
L
Löffelhardt
W
W
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002467:Cell Nucleus / Q000235:genetics; D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D011163:Hydroxymethylbilane Synthase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D008027:Light; D045342:Light-Harvesting Protein Complexes; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D018087:Plastids / Q000378:metabolism*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D014158:Transcription, Genetic
D003574:Cytochrome c Group; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D011506:Proteins; D012333:RNA, Messenger; C083951:cytochrome c553; C088752:fucoxanthin-, chlorophyll a-c-containing protein; C022302:cytochrome C-552; D011163:Hydroxymethylbilane Synthase; D012273:Ribulose-Bisphosphate Carboxylase