Polymorphism of HL +1075C, but not -480T, is associated with plasma high density lipoprotein cholesterol and apolipoprotein AI in men of a Chinese population.
Eight hundred and twenty-three Chinese Han adults aged over 40, including 466 men and 357 women were enrolled in the study to examine the association of +1075C and -480T polymorphisms in hepatic lipase gene with plasma lipoprotein and apolipoprotein levels. In this population the allele frequencies for HL +1075C and minus -480T were 0.053 and 0.362, respectively and the prevalence of HL +1075C/C and -480T/T were 0.006 and 0.132, respectively. Overall, the normal HDL-C (> or = 35 mg/dl) subjects had a higher carrier frequency of HL +1075C than the low HDL-C (<35 mg/dl) subjects (0.108 vs 0.029, P=0.039). However, when tested separately, the carrier frequency of HL +1075C was not significantly different between normal and low HDL-C females (0.101 vs 0.083, P=0.843). In males, the normal HDL-C subjects had a higher carrier frequency of HL +1075C than the low HDL-C subjects (0.113 vs 0.018, P=0.026). No significant difference of frequencies of HL -480T genotypes -480T/T,-480C/T and -480C/C was found between normal and low HDL-C subjects. Among plasma TG, TC, HDL-C, apo AI, apo AII, apo B100, apo CII, Apo CIII and apoE, only HDL-C and apo AI were significantly different among the three genotypes +1075A/A,+1075A/C and +1075C/C in men (P=0.029 and 0.032). No association was found in women. Male subjects with CC had a higher HDL-C than those with AC (P=0.020) and AA (P=0.013), AC higher than AA (P=0.017). Male subjects with CC had a higher apo AI than AC (P=0.013) and AA (P=0.019), AC higher than AA (P=0.021). Although not so significant (P=0.053) as HDL-C and apo AI, male subjects with CC had a higher apo AII than those with AC and AA. No significant difference of lipoprotein and apolipoprotein traits was found among the three -480T genotypes -480C/C,-480C/T and -480T/T, in the sample overall and in men and women separately. These results indicate that HL +1075C, not -480T polymorphism is associated with plasma high density lipoprotein cholesterol and apolipoprotein AI in men in this Chinese population.
Fang
Ding Z
DZ
Department of Biochemistry and Apolipoprotein Research Unit, West China University of Medical Sciences, Chengdu 610041 Sichuan, PR China. dzfang@mail.sc.cninfo.net
Liu
Bing W
BW
D000328:Adult; D000704:Analysis of Variance; D016632:Apolipoprotein A-I / Q000378:metabolism*; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D008076:Cholesterol, HDL / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000453:epidemiology / Q000235:genetics; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011336:Probability; D012494:Sampling Studies; D012737:Sex Factors
D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D008049:Lipase