Evidence for dominant suppression of repeat-induced point mutation (RIP) in crosses with the wild-isolated Neurospora crassa strains Sugartown and Adiopodoume-7.
A convenient assay to score repeat-induced point mutation (RIP) in Neurospora employs the erg-3 locus as a mutagenesis target. Using this assay we screened 132 wild-isolated Neurospora crassa strains for ability to dominantly suppress RIP. RIP was exceptionally inefficient in crosses with the wild isolates Sugartown (P0854) and Adiopodoume-7 (P4305), thereby suggesting the presence of dominant RIP suppressors in these strains. In other experiments, we found no evidence for dominant RIP suppression by the Spore killer haplotypes Sk-2 and Sk-3.
Noubissi
F K
FK
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
Aparna
K
K
McCluskey
K
K
Kasbekar
D P
DP
D003433:Crosses, Genetic; D005799:Genes, Dominant; D005800:Genes, Fungal; D016153:Genes, Suppressor; D006239:Haplotypes; D009492:Neurospora crassa / Q000235:genetics*; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D013172:Spores, Fungal; D019076:Transgenes