Antipoliovirus flavonoids from Psiadia dentata.
The search for antiviral agents against vesicular stomatitis virus, herpes simplex virus type 1 and poliovirus type 2 in plants extracts, led to the isolation of two antipoliovirus flavonoids from the medicinal plant Psiadia dentata (Cass.) DC, Asteraceae: 3-methylkaempferol and 3,4'-dimethylkaempferol. The antipoliovirus activity of both compounds was estimated by comparison with 3-methylquercetin, guanidine and Ro-090179. The most potent inhibitor of poliovirus replication was 3-methylkaempferol, and therefore we investigated its mechanism of action. We showed, using the inhibition of [3H]uridine incorporation in viral RNA and performing a dot-blot with one RNA probe specific for the poliovirus genomic strand RNA, that 3-methylkaempferol inhibits the genomic RNA synthesis of poliovirus.
Robin
V
V
UPRES, Pharmacochimie de Molécules de Synthèse et de Substances Naturelles, Faculté de Pharmacie, Rennes, France.
Irurzun
A
A
Amoros
M
M
Boustie
J
J
Carrasco
L
L
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D019659:Asteraceae / Q000737:chemistry*; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000633:toxicity; D044948:Flavonols; D019791:Guanidine / Q000737:chemistry / Q000494:pharmacology; D044949:Kaempferols; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D017955:Poliovirus / Q000187:drug effects* / Q000502:physiology; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
C464827:3,4'-dimethylkaempferol; C464826:3-methylkaempferol; D000998:Antiviral Agents; D005419:Flavonoids; D044948:Flavonols; D044949:Kaempferols; D012367:RNA, Viral; C047368:3-methylquercetin; C008751:pachypodol; D011794:Quercetin; D019791:Guanidine