Purification and properties of antiviral proteins from the leaves of Bougainvillea xbuttiana.
A non-phytotoxic, resistance inducing, proteinaceous antiviral principle was purified by ammonium sulphate fractionation, ion exchange chromatography and gel filtration from the leaves of Bougainvillea xbuttiana. It imparted resistance against tobacco mosaic virus (TMV) and sunnhemp rosette virus (SRV) in their respective test hosts viz. Nicotiana glutinosa, N. tabacum var. Samsun NN, and Cyamopsis tetragonoloba, respectively. The purified principle eluted as a single peak upon gel filtration, but exhibited two polypeptides on SDS-PAGE with Mr 28,000 and 24,000. The two polypeptides were found to be highly basic, rich in lysine with pI around 10.0 and 10.5, respectively. Since this principle effected local lesion inhibition in both treated and untreated top leaves of test host, it might be acting in the initial stages of virus infection as a systemic inducer.
Narwal
S
S
Division of Biochemistry, Indian Agricultural Research Institute, New Delhi.
Balasubrahmanyam
A
A
Lodha
M L
ML
Kapoor
H C
HC
D000596:Amino Acids / Q000032:analysis; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D002241:Carbohydrates / Q000032:analysis; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D014026:Nicotiana / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000187:drug effects
D000596:Amino Acids; D000998:Antiviral Agents; D002241:Carbohydrates; D010940:Plant Proteins