Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis.
The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 A. Native data have been collected to 1.6 A resolution and Hg-derivative data to 2.5 A resolution using a synchrotron-radiation source.
Mowat
Christopher G
CG
Institute of Cell and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland.
Leys
David
D
McLean
Kirsty J
KJ
Rivers
Stuart L
SL
Richmond
Alison
A
Munro
Andrew W
AW
Ortiz Lombardia
Miguel
M
Alzari
Pedro M
PM
Reid
Graeme A
GA
Chapman
Stephen K
SK
Walkinshaw
Malcolm D
MD
D003460:Crystallization; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry*
C464825:cytochrome P-450 CYP121; D003577:Cytochrome P-450 Enzyme System