title: Acute and subchronic mammalian toxicity of naphthenic acids from oil sands tailings.
abstract: Naphthenic acids are the most significant environmental contaminants resulting from petroleum extraction from oil sands deposits. In this study, a mixture of naphthenic acids isolated from Athabasca oil sands (AOS) tailings pond water was used in acute and subchronic toxicity tests with rodents, in order to assess potential risks posed to terrestrial wildlife. Dosages were chosen to bracket worst-case environmental exposure scenarios. In acute tests, adult female Wistar rats were given single po dosages of naphthenic acids at either 3, 30, or 300 mg per kg body weight (mg/kg), while adult male rats received 300 mg/kg. Food consumption was temporarily suppressed in the high-dose groups of both sexes. Following euthanasia 14 days later, histopathology revealed a significant incidence of pericholangitis in the high-dose group of both sexes, suggesting hepatotoxicity as an acute effect. Other histological lesions included brain hemorrhage in high-dose males, and cardiac periarteriolar necrosis and fibrosis in female rats. In subchronic tests, naphthenic acids were po administered to female Wistar rats at 0.6, 6, or 60 mg/kg, 5 days per week for 90 days. Results again suggested the liver as a potential target organ. The relative liver weight in the high-dose group was 35% higher than in controls. Biochemical analysis revealed elevated blood amylase (30% above controls) and hypocholesterolemia (43% below controls) in high-dose rats. Excessive hepatic glycogen accumulation was observed in 42% of animals in this group. These results indicate that, under worst-case exposure conditions, acute toxicity is unlikely in wild mammals exposed to naphthenic acids in AOS tailings pond water, but repeated exposure may have adverse health effects.
authors.affiliation: Toxicology Centre, 44 Campus Drive, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.
authors.forename: Vincent V
authors.initials: VV
authors.lastname: Rogers
authors.forename: Mark
authors.initials: M
authors.lastname: Wickstrom
authors.forename: Karsten
authors.initials: K
authors.lastname: Liber
authors.forename: Michael D
authors.initials: MD
authors.lastname: MacKinnon
mesh_terms: D000284:Administration, Oral; D000416:Alberta; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002264:Carboxylic Acids / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004326:Drinking / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D012737:Sex Factors; D023382:Toxicity Tests, Acute; D014865:Waste Disposal, Fluid
chemical_list: D002264:Carboxylic Acids; D004785:Environmental Pollutants; C046170:naphthenic acid