title: Relationship between the self-splicing activity and the solidity of the master domain of the Tetrahymena group I ribozyme.
abstract: The highly conserved P3-P7 domain of the Group I intron ribozymes is known to contain essential elements, such as the binding site for the cofactor guanosine, required for conducting the splicing reaction. We investigated the domain of the Tetrahymena intron ribozyme and its variants in order to clarify the relationship between its stability and function. We found that the destabilization of the P3-P7 domain facilitates the active structure formation at high magnesium ion concentrations where the formation is retarded for the wild type. The destabilized domain also increases K(GTP)(m) although this can be compensated by increasing the concentration of Mg(2+), indicating that the stable domain is required for establishing a tight guanosine binding site. The results suggest that the stability of the domain affects the rate-limiting step in the RNA folding pathway and also regulates the efficiency of the splicing reaction.
authors.affiliation: Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Oe
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ikawa
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shiraishi
authors.forename: Tan
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D001665:Binding Sites; D006151:Guanosine / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020871:RNA Stability; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D016053:RNA, Protozoan / Q000737:chemistry / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics* / Q000378:metabolism
chemical_list: C116167:GIR1 ribozyme; D016337:RNA, Catalytic; D016053:RNA, Protozoan; D006151:Guanosine; D008274:Magnesium