title: Functional annotation of a full-length Arabidopsis cDNA collection.
abstract: Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
authors.affiliation: Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), 3-1-1 Koyadai, Tsukuba 305-0074, Japan.
authors.forename: Motoaki
authors.initials: M
authors.lastname: Seki
authors.forename: Mari
authors.initials: M
authors.lastname: Narusaka
authors.forename: Asako
authors.initials: A
authors.lastname: Kamiya
authors.forename: Junko
authors.initials: J
authors.lastname: Ishida
authors.forename: Masakazu
authors.initials: M
authors.lastname: Satou
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Maiko
authors.initials: M
authors.lastname: Nakajima
authors.forename: Akiko
authors.initials: A
authors.lastname: Enju
authors.forename: Kenji
authors.initials: K
authors.lastname: Akiyama
authors.forename: Youko
authors.initials: Y
authors.lastname: Oono
authors.forename: Masami
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Jun
authors.initials: J
authors.lastname: Kawai
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Itoh
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ishii
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Shibata
authors.forename: Akira
authors.initials: A
authors.lastname: Shinagawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D030561:Databases, Nucleic Acid; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D018745:Genome, Plant; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger