In vivo tissue-specific regulation of the human papillomavirus type 18 early promoter by estrogen, progesterone, and their antagonists.
Human papillomavirus type 18 is a causative agent of epithelial cancers in the uterine cervix. We show here that estrogen and progesterone activate beta-galactosidase expression from the early promoter of this virus in the genital epithelia of transgenic mice. Ovariectomy caused suppression of transgene expression exclusively in vagina and cervix epithelia. Beta-galactosidase expression could be restored in ovariectomized females by administration of estrogen, alone or in combination with progesterone. Further, rescue of transgene expression was inhibited by the estrogen antagonist tamoxifen and the anti-progesterone RU486, suggesting that this was a specific effect.
Morales-Peza
Néstor
N
Institute of Biomedical Research, National Autonomous University of Mexico (UNAM), 04510 Mexico City, Mexico.
Auewarakul
Prasert
P
Juárez
Victoria
V
García-Carrancá
Alejandro
A
Cid-Arregui
Angel
A
D000818:Animals; D002584:Cervix Uteri / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D030762:Estrous Cycle; D005260:Female; D015967:Gene Expression Regulation, Viral; D006727:Hormone Antagonists / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015735:Mifepristone / Q000494:pharmacology; D010052:Ovariectomy; D027383:Papillomaviridae / Q000187:drug effects / Q000235:genetics*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D013629:Tamoxifen / Q000494:pharmacology; D019076:Transgenes; D014621:Vagina / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D006727:Hormone Antagonists; D014764:Viral Proteins; D013629:Tamoxifen; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol; D001616:beta-Galactosidase