DNA repair dependent NAD+ metabolism is impaired in cells from patients with Fanconi's anemia.
In vitro cultivated fibroblasts derived either from patients with Fanconi's anemia (FA) or from healthy probands were analyzed for their DNA repair-dependent NAD+ metabolism. No difference in NAD+ pools was found. NAD+ consumption after cell damage by u.v. irradiation was, however, significantly reduced in FA cells. Several FA cell lines had a lowered ability to transfer ADP-ribose to acid-precipitable material. Additionally, a decreased activity of NAD: protein ADP-ribosyltransferase was found for three FA cell lines. Our data indicate, that FA is accompanied by a defective NAD+ metabolism during DNA repair.
Klocker
H
H
Institut für Biochemie (Nat. Fak.), Universität Innsbruck, Austria.
Auer
B
B
Hirsch-Kauffmann
M
M
Altmann
H
H
Burtscher
H J
HJ
Schweiger
M
M
D002457:Cell Extracts; D002463:Cell Membrane Permeability; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D009243:NAD / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014316:Tritium; D014466:Ultraviolet Rays
D002457:Cell Extracts; D009243:NAD; D014316:Tritium; D011065:Poly(ADP-ribose) Polymerases