Regulation of DNA replication in the nuclei of the slime mold Physarum polycephalum. Transplantation of nuclei by plasmodial coalescence.
Nuclei in G2 phase of the slime mold Physarum polycephalum, when transplanted, by plasmodial coalescence, into an S-phase plasmodium, failed to start another round of DNA synthesis. In the reciprocal combination, S-phase nuclei in a G2-phase host continued DNA synthesis for several hours without appreciable decrease in rate. It is suggested that the beginning of DNA replication is determined by an event, either during or shortly after mitosis, which renders the chromosomes structurally competent for DNA replication.
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Department of Biology, Loyola University, Chicago, Illinois 60626, USA.
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D000818:Animals; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D004247:DNA / Q000096:biosynthesis*; D016195:G2 Phase; D008938:Mitosis; D016839:Physarum polycephalum / Q000235:genetics* / Q000502:physiology*; D016196:S Phase; D013997:Time Factors
D004247:DNA