[Modification of the Bilshovsky method for studies of nerve tissue paraffin sections].
The Bilshovsky method is often used for studies of the morphology of neuronal fibrillar system. The classical variant of this method requires sections made with a freezing microtome, which is sometimes inconvenient. Preparation of paraffin sections often gives unsatisfactory results. The authors managed to overcome this deficiency. Their modification consists in the following: after deparaffinization of paraffin sections glued to the slides they are repeatedly fixed in warm formalin and then treated with silver nitrate with heating. This modification is easily reproducible and essentially simplifies staining; no freezing microtome is needed.
Sukhanov
A V
AV
Vakulin
G M
GM
D000544:Alzheimer Disease / Q000473:pathology; D006801:Humans; D008297:Male; D009417:Nerve Tissue / Q000166:cytology* / Q000648:ultrastructure; D009454:Neurofibrils / Q000648:ultrastructure; D016612:Paraffin Embedding; D013788:Thalamus / Q000648:ultrastructure