title: [Resistance tests in general practice. Validity can be improved by standardized procedures].
abstract: BACKGROUND
  Susceptibility testing of bacteria in urine is one of the commonest laboratory tests in general practice in Denmark. It is quick and easy to perform, but recent studies have shown low validity when the test is performed in general practice. If it is to continue as a diagnostic tool in general practice, its quality should be improved. The aim of this study was to investigate the effect of an intervention to improve the quality of susceptibility testing in general practice.
  
  
  METHODS
  Twenty-three randomly selected general practices took part in the study. The intervention consisted of visits by laboratory technicians who instructed the practitioners in standardised procedures for susceptibility testing. Before and after the intervention, urine specimens containing monocultures of typical uropathogenic bacteria were sent to the practices. The practitioners performed susceptibility testing by the Sensicult and the Iso-Resagar methods, and the validity of the results before and after the intervention was compared. Results from susceptibility testing at the bacteriological laboratory, Odense University Hospital, were used as the gold standard.
  
  
  RESULTS
  The median frequency of correct results increased from 82% to 98% for susceptibility testing by the Sensicult method (p = 0.001) and from 90% to 96% by the Iso-Resagar method (p = 0.05).
  
  
  CONCLUSIONS
  The validity of susceptibility testing in general practice improves when preceded by instruction in standardised procedures.
authors.affiliation: Forskningsenheden for Almen Medicin, Syddansk Universitet, Winsløwparken 19, DK-5000 Odense. lbjerrum@health.sdu.dk
authors.forename: Lars
authors.initials: L
authors.lastname: Bjerrum
authors.forename: Per
authors.initials: P
authors.lastname: Grinsted
authors.forename: Per Hyltoft
authors.initials: PH
authors.lastname: Petersen
authors.forename: Per
authors.initials: P
authors.lastname: Søgaard
mesh_terms: D000892:Anti-Infective Agents, Urinary / Q000627:therapeutic use; D024881:Drug Resistance, Bacterial; D005194:Family Practice; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D015203:Reproducibility of Results; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology / Q000652:urine
chemical_list: D000892:Anti-Infective Agents, Urinary