title: Chemokine activation within 24 hours after blunt accident trauma.
abstract: Chemokines mediate the migration of leukocytes to sites of inflammation. Changes in the plasma concentration of interleukin (IL)-8 and macrophage inflammatory protein (MIP)-1beta have not been investigated in the very early phase starting immediately after unintentional trauma. Enrolled in the study were 94 patients with multiple blunt injuries. Blood samples were collected at the scene of accident, then at regular intervals for 24 h. IL-8 and MIP-1beta plasma levels were determined by commercial test kits. Patients were grouped according to trauma severity, pattern of injury, as well as survivors vs. nonsurvivors. Serious casualties [Injury Severity Score (ISS) > or = 32] revealed a significant increase in IL-8 compared to only a slight elevation in individuals with an ISS < 32. Nonsurvivors showed a highly significant (P < 0.005) increase in IL-8 levels beginning immediately after admission. Trauma resulted in a modest activation of MIP-1beta production without differences regarding trauma severity, pattern of injury, or survival. A very strong trauma stimulus is necessary to activate IL-8 production. In contrast to MIP-1beta, IL-8 levels were significantly elevated in nonsurvivors compared to survivors. Therefore, IL-8 might be an early predictor of survival.
authors.affiliation: Department of Trauma, Hand, and Reconstructive Surgery, University of Ulm, Germany.
authors.forename: Ulrich C
authors.initials: UC
authors.lastname: Liener
authors.forename: Uwe B
authors.initials: UB
authors.lastname: Brückner
authors.forename: Markus W
authors.initials: MW
authors.lastname: Knöferl
authors.forename: Gerald
authors.initials: G
authors.lastname: Steinbach
authors.forename: Lothar
authors.initials: L
authors.lastname: Kinzl
authors.forename: Florian
authors.initials: F
authors.lastname: Gebhard
mesh_terms: D000059:Accidents; D000293:Adolescent; D000328:Adult; D000368:Aged; D054407:Chemokine CCL4; D005260:Female; D006801:Humans; D015601:Injury Severity Score; D016209:Interleukin-8 / Q000097:blood*; D019402:Macrophage Inflammatory Proteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D015996:Survival Rate; D014949:Wounds, Nonpenetrating / Q000097:blood* / Q000145:classification* / Q000401:mortality
chemical_list: D054407:Chemokine CCL4; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins