Hypothermia inhibits translocation of CaM kinase II and PKC-alpha, beta, gamma isoforms and fodrin proteolysis in rat brain synaptosome during ischemia-reperfusion.
To clarify the involvement of intracellular signaling pathway and calpain in the brain injury and its protection by mild hypothermia, immunoblotting analyses were performed in the rat brain after global forebrain ischemia and reperfusion. After 30 min of ischemia followed by 60 min of reperfusion, Ca2+/calmodulin-dependent kinase II (CaM kinase II) and protein kinase C (PKC)-alpha, beta, gamma isoforms translocated to the synaptosomal fraction, while mild hypothermia (32 degrees C) inhibited the translocation. The hypothermia also inhibited fodrin proteolysis caused by ischemia-reperfusion, indicating the inhibition of calpain. These effects of hypothermia may explain the mechanism of the protection against brain ischemia-reperfusion injury through modulating synaptosomal function.
Harada
Kazuki
K
Department of Emergency and Critical Care Medicine, Yamaguchi University School of Medicine, Yamaguchi, Japan.
Maekawa
Tsuyoshi
T
Tsuruta
Ryosuke
R
Kaneko
Tadashi
T
Sadamitsu
Daikai
D
Yamashima
Tetsumori
T
Yoshida Ki
Ken-ichi
K
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002545:Brain Ischemia / Q000201:enzymology* / Q000503:physiopathology / Q000628:therapy; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002154:Calpain / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D007036:Hypothermia, Induced; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology* / Q000503:physiopathology / Q000628:therapy; D013347:Subcellular Fractions / Q000201:enzymology; D013574:Synaptosomes / Q000201:enzymology*; D013049:Spectrin
D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D002154:Calpain; D002352:Carrier Proteins; D007527:Isoenzymes; D008840:Microfilament Proteins; D010447:Peptide Hydrolases; D020033:Protein Isoforms; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D013049:Spectrin; C087857:protein kinase C gamma