Varicella-zoster virus open reading frame 2 encodes a membrane phosphoprotein that is dispensable for viral replication and for establishment of latency.
Varicella-zoster virus (VZV) encodes six genes that do not have homologs in herpes simplex virus. One of these genes, VZV open reading frame 2 (ORF2), was expressed as a 31-kDa phosphoprotein in the membranes of infected cells. Unlike equine and bovine herpesvirus type 1 ORF2 homologs that are associated with virions, VZV virions contained no detectable ORF2 protein. The ORF2 deletion mutant established a latent infection in cotton rats at a frequency and with a number of VZV genomes similar to that of the parental virus. ORF63 transcripts, a hallmark of latent infection, were present in ganglia latently infected with both the ORF2 deletion mutant and parental VZV. Thus, ORF2 is the first VZV gene shown to be dispensable for establishment of latent infection in an animal model.
Sato
Hitoshi
H
Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-1888, USA.
Pesnicak
Lesley
L
Cohen
Jeffrey I
JI
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D017353:Gene Deletion; D014645:Herpesvirus 3, Human / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D016366:Open Reading Frames; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D016205:Sigmodontinae; D017735:Virus Latency; D014779:Virus Replication
D008565:Membrane Proteins; D010750:Phosphoproteins