title: An improved polymerase chain reaction assay for the detection of Tritrichomonas foetus in cattle.
abstract: An improved polymerase chain reaction test has been developed to detect Tritrichomonas foetus, the causative agent of trichomoniasis in cattle. The test amplifies a region of the 5.8S ribosomal RNA gene of T. foetus, and it is simple, sensitive, and specific when compared with traditional methods to examine field samples.
authors.affiliation: Department of Biochemistry and Molecular Biology, Health Sciences Centre, 3330 Hospital Drive NW, University of Calgary, Calgary, Alberta T2N 4N1.
authors.forename: D Douglas
authors.initials: DD
authors.lastname: Nickel
authors.forename: Merle E
authors.initials: ME
authors.lastname: Olson
authors.forename: Gilbert A
authors.initials: GA
authors.lastname: Schultz
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011528:Protozoan Infections / Q000175:diagnosis / Q000469:parasitology; D011529:Protozoan Infections, Animal; D016053:RNA, Protozoan / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016835:Tritrichomonas foetus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D016053:RNA, Protozoan