title: Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression.
abstract: Specific recruitment of corepressor complexes containing histone deacetylases (HDAC) by transcription factors is believed to play an essential role in transcriptional repression. Recent studies indicate that repression by unliganded nuclear hormone receptors and by the Mad family of repressors requires distinct HDAC-containing corepressor complexes. In this work, we show that unliganded TR specifically recruits only the closely related N-CoR and SMRT-HDAC3 complexes, whereas the Mad1 recruits only the Sin3-HDAC1/2 complex. Significantly, both the Sin3 and Mi-2/NURD complexes also exhibit constitutive association with chromatin and contribute to chromatin deacetylation in a nontargeted fashion. These results suggest that HDAC complexes can contribute to gene repression by two distinct mechanisms as follows: (1) specific targeting by repressors and (2) constitutive association with chromatin.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Li
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Lin
authors.forename: Weidong
authors.initials: W
authors.lastname: Wang
authors.forename: Paul
authors.initials: P
authors.lastname: Wade
authors.forename: Jiemin
authors.initials: J
authors.lastname: Wong
mesh_terms: D000107:Acetylation; D000251:Adenosine Triphosphatases; D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D015153:Blotting, Western; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004265:DNA Helicases; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases / Q000737:chemistry* / Q000378:metabolism*; D008024:Ligands; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014981:Xenopus
chemical_list: D001324:Autoantigens; C115323:CHD4 protein, human; D002843:Chromatin; D008024:Ligands; D012097:Repressor Proteins; C071731:SIN3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C110550:HDAC1 protein, human; D056284:Histone Deacetylase 1; D056464:Histone Deacetylase 2; D006655:Histone Deacetylases; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D000251:Adenosine Triphosphatases; D004265:DNA Helicases