Ion chromatographic separation and determination of phosphate and arsenate in water and hair.
A simple and sensitive method for the sequential determination of phosphate and arsenate was developed based on initial ion chromatographic separation followed by detection as the ion-association complex formed by heteropolymolybdophosphate and arsenate with bismuth. With 200 microl sample injection and separation on a AS4A-SC column using an eluent of 3.5 mM sodium hydrogen carbonate-10.0 mM sodium hydroxide, the detection limits which are calculated as the concentration equivalent to twice the baseline noise, were found to be 0.8 microg/l and 4.2 microg/l for P and As, respectively. Spiked samples were analyzed and recoveries were found to be satisfactory in the range of 95-105% for phosphate and 90-105% for arsenate. Samples of water and hair were analyzed by the proposed method.
Antony
P J
PJ
Centre for Marine Analytical Reference and Standards (C-MARS), Regional Research Laboratory (CSIR), Thiruvananthapuram, India.
Karthikeyan
S
S
Iyer
C S P
CS
D001149:Arsenates / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D006801:Humans; D010710:Phosphates / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014867:Water / Q000737:chemistry*
D001149:Arsenates; D010710:Phosphates; D014867:Water; C025657:arsenic acid