title: Cross-reactivity and sensitivity of two Legionella urinary antigen kits, Biotest EIA and Binax NOW, to extracted antigens from various serogroups of L. pneumophila and other Legionella species.
abstract: Legionella antigen detection kits for diagnosing legionellosis from urine have become widely used, but basic information about reactivity of the kits to non-serogroup (SG) 1 L. pneumophila and other Legionella species remains incomplete. We evaluated Biotest EIA and the most recently developed Binax NOW by using in-vitro extracted antigens of 22 L. pneumophila SG 1 to 15 strains and of 27 other Legionella species. Both kits showed excellent sensitivity to L pneumophila SG 1 antigens, but reacted to different sets of non-SG I L. pneumophila with different sensitivity. No cross-reactivity was observed to Legionella species other than L. pneumophila.
authors.affiliation: Laboratory of Clinical Microbiology, Toranomon Hospital, Tokyo, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Okada
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Kura
authors.forename: Akihito
authors.initials: A
authors.lastname: Wada
authors.forename: Hiroko
authors.initials: H
authors.lastname: Inagawa
authors.forename: Gang-Hong
authors.initials: GH
authors.lastname: Lee
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsushita
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification / Q000652:urine*; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000145:classification / Q000276:immunology*; D016952:Legionella pneumophila / Q000145:classification / Q000276:immunology; D007876:Legionellosis / Q000175:diagnosis*; D007877:Legionnaires' Disease / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D011933:Reagent Kits, Diagnostic