title: [Successful direct transfer of a deep frozen-thawed equine embryo].
abstract: Embryos were flushed on day 7 after ovulation from two mares, and frozen using a conventional slow freezing procedure in phosphate buffered (PBS) saline supplemented with 10% FCS, 1.5 mol/L ethylene glycol and 0.25 mol/L sucrose. One of the two embryos was thawed after 10 months of storage in liquid nitrogen and transferred directly (without dilution of the cryoprotectant and quality examination) to a synchronized recipient. This transfer resulted in the birth of a live female foal. To our knowledge, this is the first live foal born after direct transfer of a frozen-thawed equine embryo.
authors.affiliation: Interuniversitäres Forschungsinstitut für Agrarbiotechnologie, Tulln, Osterreich. MNowshari@hotmail.com
authors.forename: P
authors.initials: P
authors.lastname: Ulrich
authors.forename: M A
authors.initials: MA
authors.lastname: Nowshari
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D006736:Horses / Q000502:physiology*; D011247:Pregnancy