title: Effect of blood transfusion in an experimental sarcoma model.
abstract: OBJECTIVE
  To study the effect of allogeneic, syngeneic, and autologous blood transfusion on the growth rate of the KHT tumor in a C3H murine model.
  
  
  METHODS
  Prospective, randomized, and controlled animal study.
  
  
  METHODS
  Sixty-one C3H female mice.
  
  
  METHODS
  The C3H female mice were implanted with 2 x 10(5) cells of KHT, a murine sarcoma. Ten days later, 0.3 mL of blood was removed from a retro-orbital site to simulate surgical blood loss. This blood loss was replaced by blood transfusion through a tail vein with the use of allogeneic (major histocompatibility complex incompatible), syngeneic (major histocompatibility complex compatible), or autologous blood. Tumor growth was measured daily for 14 days. The tumor growth curve for each of the animals was constructed and the mean slope of growth calculated for each group.
  
  
  RESULTS
  There were statistically significant differences in tumor growth rate (P =.001) when the allogeneic group (mean slope = 0.232, n = 14), the syngeneic group (mean slope = 0.190, n = 17), and the autologous group (mean slope = 0.202, n = 14) were compared. A t test confirmed that there was no significant difference in the tumor growth rate between the groups transfused with syngeneic and autologous blood (P =.26). However, the rate of tumor growth in the allogeneic group was found to be significantly higher when independently compared with the syngeneic group (P<.001) and the autologous group (P =.02).
  
  
  CONCLUSIONS
  In this experimental model of a solid murine sarcoma, allogeneic blood transfusion was associated with an increased rate of tumor growth compared with syngeneic and autologous blood transfusion, likely reflecting immunomodulatory effects incurred by the introduction of major histocompatibility complex-incompatible antigens.
authors.affiliation: Division of Otolaryngology/Head & Neck Surgery, Edwards Building, R135, Stanford University Medical Center, Stanford, CA 94305-5328, USA.
authors.forename: Ho-Sheng
authors.initials: HS
authors.lastname: Lin
authors.forename: Ravi N
authors.initials: RN
authors.lastname: Samy
authors.forename: Joanne
authors.initials: J
authors.lastname: Lum
authors.forename: Mary Jo
authors.initials: MJ
authors.lastname: Dorie
authors.forename: David J
authors.initials: DJ
authors.lastname: Terris
mesh_terms: D000818:Animals; D001804:Blood Transfusion, Autologous / Q000009:adverse effects; D005260:Female; D006648:Histocompatibility; D051379:Mice; D008809:Mice, Inbred C3H; D011446:Prospective Studies; D011897:Random Allocation; D012513:Sarcoma, Experimental / Q000473:pathology*; D065227:Transfusion Reaction