AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis.
In contrast to animals, little is known about pattern formation in plants. Physiological and genetic data suggest the involvement of the phytohormone auxin in this process. Here, we characterize a novel member of the PIN family of putative auxin efflux carriers, Arabidopsis PIN4, that is localized in developing and mature root meristems. Atpin4 mutants are defective in establishment and maintenance of endogenous auxin gradients, fail to canalize externally applied auxin, and display various patterning defects in both embryonic and seedling roots. We propose a role for AtPIN4 in generating a sink for auxin below the quiescent center of the root meristem that is essential for auxin distribution and patterning.
Friml
Jirí
J
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, Köln, Germany.
Benková
Eva
E
Blilou
Ikram
I
Wisniewska
Justyna
J
Hamann
Thorsten
T
Ljung
Karin
K
Woody
Scott
S
Sandberg
Goran
G
Scheres
Ben
B
Jürgens
Gerd
G
Palme
Klaus
K
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D017403:In Situ Hybridization; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins; D018519:Meristem / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000378:metabolism*; D012639:Seeds / Q000033:anatomy & histology / Q000502:physiology; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C451260:PIN4 protein, Arabidopsis