Roscovitine inhibits ongoing DNA synthesis in human cervical cancer.
The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 microM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed.
Vitali
Liliana
L
Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', FCEyN, UBA, Patricias Argentinas 435 (C. P. 1405), Buenos Aires, Argentina.
Yakisich
J Sebastian
JS
Vita
Marina Fernanda
MF
Fernandez
Alejandro
A
Settembrini
Liliana
L
Siden
Ake
A
Cruz
Mabel
M
Carminatti
Hector
H
Casas
Oscar
O
Idoyaga Vargas
Victor
V
D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000494:pharmacology; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006801:Humans; D008875:Middle Aged; D011687:Purines / Q000494:pharmacology*; D000077546:Roscovitine; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D011687:Purines; D000077546:Roscovitine; D004247:DNA; D018844:Cyclin-Dependent Kinases