title: Quantitative determination of glufosinate in biological samples by liquid chromatography with ultraviolet detection after p-nitrobenzoyl derivatization.
abstract: We have established a new HPLC method for derivatizing and quantifying glufosinate (GLUF) in human serum and urine using p-nitrobenzoyl chloride (PNBC). The p-nitrobenzoyl derivative of GLUF (PNB-GLUF) was produced quantitatively over 10 min at room temperature. PNB-GLUF possesses the property of ultraviolet (UV) light absorption with a lambda(max) of 272.8 nm, and was isolated from biological specimens by reversed-phase chromatography using Inertsil Ph-3. In experiments at a UV wavelength of 273 nm, GLUF has a quantitative detection limit of 0.005 microg/ml, and when it was added to both serum and urine to yield concentrations of 0.1-1000 microg/ml, its recovery rate was quite satisfactory: at least 93.8% in all cases. Further, the measured amounts of GLUF in 23 serum samples from patients intoxicated by ingestion of GLUF compared favorably with those obtained by fluorescence derivatization-HPLC using 9-fluorenylmethyl chloroformate (R=0.998). This technique of analysis is, in addition, applicable for Glyphosat, which possesses a chemical structure resembling that of GLUF, and it will be of great use in the determination of these two compounds.
authors.affiliation: Department of Analytical Chemistry, Niigata College of Pharmacy, Japan. horiy@xa3.so-net.ne.jp
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hori
authors.forename: Manami
authors.initials: M
authors.lastname: Fujisawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Shimada
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Sato
authors.forename: Michio
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Masao
authors.initials: M
authors.lastname: Honda
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hirose
mesh_terms: D000613:Aminobutyrates / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000613:Aminobutyrates; C003121:phosphinothricin