Cloning of bovine eukaryotic translation initiation factor 4E (eIF-4E) and its expression in the bovine mammary gland at different physiological stages.
Bovine eIF-4E cDNA was cloned and its expression in the bovine mammary gland at distinct physiological stages was investigated. Bovine eIF-4E cDNA and protein sequences are highly homologous to those from other mammals. Using Northern blot analysis, we did not detect eIF-4E expression in the prepubertal bovine mammary gland, whereas a low level of eIF-4E mRNA was observed in the mammary tissues of heifers during the third estrous cycle. The eIF-4E mRNA level was significantly higher in the lactating mammary gland compared to the mammary tissues obtained from heifers during the third estrous cycle. Alteration of eIF-4E expression in bovine mammary tissues during different physiological stages indicates the involvement of eIF-4E in mammary development. Elevated eIF-4E expression during lactation may be related to increased translation of certain mRNA or the acceleration of overall protein synthesis.
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Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada.
Capuco
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AV
Zhao
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D039561:Eukaryotic Initiation Factor-4E; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007774:Lactation / Q000235:genetics; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics* / Q000378:metabolism; D017385:Sequence Homology
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors