A comparison of the metabolic effects of continuous hypothermic perfusion or oxygenated persufflation during hypothermic storage of rat liver.
The metabolic consequences of supplying oxygen by two different modes were investigated. The effects of hypothermic liver preservation after cold hypoxic flush (Group I), oxygenated vascular persufflation (Group II), and continuous oxygenated perfusion (Group III) were compared. Adenine nucleotides were measured to assess energetics, and 1H nuclear magnetic resonance spectroscopy was employed to investigate other metabolic pathways. Energetics were maintained by both modes of oxygenation at 24 h. The mitochondrial redox state is indicated by the ratio of acetoacetate (Ace) and beta-hydroxybutyrate (betaHb). The detection of only betaHb or Ace in the hypoxic flush and perfused livers, respectively, suggested that the mitochondria of these livers were hyperreduced and hyperoxidized, respectively. In contrast, both components of the redox couple were detected in the persufflated livers, suggesting that persufflation may be a simple and effective method of maintaining hepatic energetics long-term while maintaining a more normal mitochondrial redox state.
So
P W
PW
University Department of Surgery, Royal Free Hospital School of Medicine, Pond Street, London NW3 2QG, United Kingdom.
Fuller
B J
BJ
D000227:Adenine Nucleotides / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D004734:Energy Metabolism; D005650:Fumarates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007657:Ketone Bodies / Q000378:metabolism; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry; D019802:Succinic Acid / Q000378:metabolism; D014021:Tissue Preservation / Q000379:methods*
D000227:Adenine Nucleotides; D000596:Amino Acids; D005650:Fumarates; D007657:Ketone Bodies; D019344:Lactic Acid; C032005:fumaric acid; D019802:Succinic Acid; D005947:Glucose; D010100:Oxygen