Identification of anticoagulant activities in salivary gland extracts of four horsefly species (Diptera, tabanidae).
Anticoagulant activities against the extrinsic and intrinsic coagulation pathways were identified in salivary gland extracts (SGE) prepared from four tabanids (Hybomitra muehlfeldi, Tabanus autumnalis, Haematopota pluvialis, Heptatoma pellucens). All extracts prolonged human plasma clotting time in a dose-dependent manner and inhibited thrombin activity in the chromogenic substrate assay. Horsefly SGE did not inhibit factor Xa. Partial purification of SGE proteins using reversed-phase high-performance liquid chromatography revealed species-specific differences in the elution profiles and range of fractions with anticoagulant activities.
Kazimírová
M
M
Institute of Zoology, Slovak Academy of Sciences, SK-84206 Bratislava, Slovakia. uzaemkaz@savba.sk
Sulanová
M
M
Kozánek
M
M
Takác
P
P
Labuda
M
M
Nuttall
P A
PA
D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification* / Q000494:pharmacology; D001180:Arthropod Venoms / Q000302:isolation & purification* / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D002457:Cell Extracts; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000737:chemistry; D015951:Factor Xa / Q000187:drug effects; D006801:Humans; D012469:Salivary Glands / Q000737:chemistry*; D013045:Species Specificity; D013917:Thrombin / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D001180:Arthropod Venoms; D002457:Cell Extracts; D013917:Thrombin; D015951:Factor Xa