Radiation responsiveness of human lung cancer cell lines measured with a short term semiautomatic assay.
BACKGROUND
Fluorometric microculture cytotoxicity assay (FMCA) is a short-term semi-automatic method, based on dye-inclusion of surviving cells. The assay was developed for investigations of drug resistance on tumour cells from biopsy material. In the present study, this short-term assay was evaluated, regarding usefulness in determining radio-sensitivity.


METHODS
Eight human lung cancer cell lines were used. There were five small cell lung cancer (SCLC and three non-small cell lung cancer (NSCLC cell lines. Results were compared with the corresponding data derived from the clonogenic assay and/or the extrapolation method.


RESULTS
The surviving fraction (SF) after 2, 5 and 10 Gy compared with data from the clonogenic assay were not in accordance for 5 of the 8 cell lines. The FMCA assay overestimated SF- values for the SCLC cell lines.


CONCLUSIONS
The FMCA assay is not useful as a quick screening method for the radioresponsiveness in vitro of human tumour cell lines.
Xing
T
T
Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Sweden.
Brattstrom
D
D
Bergqvist
M
M
Isaksson
U
U
Wagenius
G
G
Brodin
O
O
D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000532:radiotherapy*; D018288:Carcinoma, Small Cell / Q000473:pathology / Q000532:radiotherapy*; D002455:Cell Division / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured