title: An origin of DNA replication from streptomycete phage phi U1.
abstract: A DNA fragment from phage phi U1 containing an origin of DNA replication was identified. This fragment, designated ori, was able to support the maintenance in Streptomyces lividans of a plasmid lacking a functional Gram-positive ori. The sequence of the minimal ori fragment was determined and analyzed. The minimal fragment conferring replication origin function contained a number of direct and inverted repeats. The absence of an open reading frame in this ori fragment indicates that host factors alone were sufficient to initiate replication at ori.
authors.affiliation: Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Farkasovská
authors.forename: A
authors.initials: A
authors.lastname: Godány
authors.forename: G
authors.initials: G
authors.lastname: Bukovská
authors.forename: J
authors.initials: J
authors.lastname: Timko
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication; D004279:DNA, Viral / Q000502:physiology*; D008969:Molecular Sequence Data; D018741:Replication Origin; D013302:Streptomyces / Q000821:virology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral