title: The Autosow raised miniature swine as a model for assessing the effects of dietary soy trypsin inhibitor.
abstract: Toxicological effects of dietary soy trypsin inhibitor (TI) were assessed in male miniature swine, a model chosen for its similarities to human digestive physiology and anatomy. The TI preparation was extracted from defatted raw soy flour. From 1 through 5 weeks of age, piglets were automatically fed either a TI liquid diet [Autosow TI group (ASTI)] or a control liquid diet [Autosow control group (ASC)]. From 6 to 39 weeks of age, these animals received either swine chow and TI or swine chow and control article. The TI diets were formulated to contain a TI activity of approximately 500 mg TI/100 g dry matter. A sow control (SC) group suckled from birth to 6 weeks of age and then fed as the ASC group with swine chow plus control article from 6 to 39 weeks of age. The SC piglets grew faster than ASC piglets during postnatal weeks 1 and 2; however, the ASC piglets were significantly heavier than the SC piglets (P=0.001) at 6 weeks of age. Compared with the ASC group, TI caused a moderate decrease in feed consumption and a moderate but reversible decrease in growth from 2 to 5 weeks of age, but not thereafter. Some control and TI-fed Autosow-reared piglets had loose stools until 6 weeks of age; the effect was significantly greater in the TI-fed group. Otherwise, all swine were active and had normal appearance and behavior.
authors.affiliation: US Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Toxicological Research and Nutritional Product Studies, Muirkirk Research Center, 8301 Muirkirk Road, Laurel, MD 20708, USA. lgarthof@cfsan.fda.gov
authors.forename: L H
authors.initials: LH
authors.lastname: Garthoff
authors.forename: G R
authors.initials: GR
authors.lastname: Henderson
authors.forename: A O
authors.initials: AO
authors.lastname: Sager
authors.forename: T J
authors.initials: TJ
authors.lastname: Sobotka
authors.forename: R
authors.initials: R
authors.lastname: O'Dell
authors.forename: C W
authors.initials: CW
authors.lastname: Thorpe
authors.forename: W J
authors.initials: WJ
authors.lastname: Trotter
authors.forename: V R
authors.initials: VR
authors.lastname: Bruce
authors.forename: H L
authors.initials: HL
authors.lastname: Dallas
authors.forename: P L
authors.initials: PL
authors.lastname: Poelma
authors.forename: H M
authors.initials: HM
authors.lastname: Solomon
authors.forename: J W
authors.initials: JW
authors.lastname: Bier
authors.forename: M W
authors.initials: MW
authors.lastname: O'Donnell
authors.forename: R K
authors.initials: RK
authors.lastname: Chi
authors.forename: S J
authors.initials: SJ
authors.lastname: Chirtel
authors.forename: C N
authors.initials: CN
authors.lastname: Barton
authors.forename: L H
authors.initials: LH
authors.lastname: Brown
authors.forename: V P
authors.initials: VP
authors.lastname: Frattali
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D003967:Diarrhea / Q000209:etiology / Q000662:veterinary; D004032:Diet; D004195:Disease Models, Animal; D005247:Feeding Behavior; D005260:Female; D008297:Male; D010940:Plant Proteins / Q000009:adverse effects*; D030262:Soybean Proteins / Q000737:chemistry*; D013552:Swine; D014361:Trypsin Inhibitors; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D010940:Plant Proteins; D030262:Soybean Proteins; D014361:Trypsin Inhibitors; D000516:alpha-Amylases