Antimicrobial synergistic effect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus.
The antimicrobial effect of linolenic acid with or without monoglyceride (glycerol laurate or glycerol myristate) against six food-borne microorganisms was determined in broth medium. Minimum inhibitory concentration of linolenic acid on Bacillus cereus and Staphylococcus aureus was 20 and 50 ppm, respectively. The growth of B. cereus treated with linolenic acid at 10 ppm with 10 ppm monoglyceride was more inhibitory than that of linolenic acid alone, and the viable cell population was reduced 2-4 log cycles compared to that of the control. When linolenic acid was added at that level, the adenosine triphosphate (ATP) concentration of extracellular fluid was drastically increased compared with that of the control, and the combined effect with monoglyceride was higher than that with linolenic acid alone. However, the intracellular ATP concentration decreased compared with that of the control. From these results, we concluded that linolenic acid has a strong antimicrobial activity against B. cereus and S. aureus, and that linolenic acid combined with monoglyceride showed stronger antimicrobial activity than using linolenic acid alone.
Lee
Ji-Young
JY
Food Science and Technology, Faculty of Biotechnology, Chonbuk National University, Dukjin-Dong, Jeonju, Jeonbuk 561-756, Republic of Korea.
Kim
Yong-Suk
YS
Shin
Dong-Hwa
DH
D000255:Adenosine Triphosphate / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects; D005079:Excipients / Q000008:administration & dosage / Q000494:pharmacology; D005110:Extracellular Space / Q000737:chemistry; D005516:Food Microbiology; D005989:Glycerides / Q000008:administration & dosage / Q000494:pharmacology*; D012477:Salmonella enteritidis / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D014736:Vibrio parahaemolyticus / Q000187:drug effects; D017962:alpha-Linolenic Acid / Q000008:administration & dosage / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D005079:Excipients; D005989:Glycerides; D017962:alpha-Linolenic Acid; D000255:Adenosine Triphosphate