[Chromosome instability in women with genital organs carcinoma].
OBJECTIVE
The aim of the current work was the assessment of the mutagen susceptibility of chromosomes of patients with carcinomas in comparison to healthy volunteers. It was interesting whether the bleomycin assay can be useful for searching for more susceptible to cancer disease individuals.


METHODS
There were 4 groups of patients analysed: controls and three test groups (patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma). In total 108 female patients were examined by use of bleomycin assay. Lymphocytes were cultured in vitro and treated with bleomycin. The b/c (breaks per cell) index was evaluated by use of light microscopy.


RESULTS
Statistically significant increased test values were found in patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma.


CONCLUSIONS
The assessment of chromosome instability could be a useful prognostic test in the diagnosis of carcinoma of female genital organs. The bleomycin assay is useful for searching subpopulations with higher chromosome instability and more susceptible to cancer disease.
Waksmański
B
B
II Katedry i Kliniki Połoznictwa i Ginekologii SlAM w Zabrzu.
Dudkiewicz
J
J
Srebrniak
M
M
D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000097:blood*; D001761:Bleomycin / Q000494:pharmacology*; D016022:Case-Control Studies; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000187:drug effects*; D016889:Endometrial Neoplasms / Q000235:genetics; D005260:Female; D005833:Genital Neoplasms, Female / Q000097:blood / Q000188:drug therapy / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D016296:Mutagenesis; D009152:Mutagenicity Tests; D010051:Ovarian Neoplasms / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D001761:Bleomycin