title: Small-caliber mesothelial cell-layered polytetraflouroethylene vascular grafts in New Zealand white rabbits.
abstract: Reduction in the thrombogenicity of small-caliber synthetic vascular grafts by lining them with mesothelial cell has been suggested as a method to reduce thrombosis. The purpose of this research is to determine whether creation of a mesothelial lining on the inner surface of a synthetic vascular graft would improve the patency rate of a small-caliber vascular grafts. Carotid interposition grafting was performed using mesothelial-lined grafts (MLG) in 30 New Zealand rabbits and compared with similar carotid interposition grafts using non-mesothelial-lined grafts (NLG) on the contralateral side. The mesothelial lining was created by suturing a piece of harvested peritoneum with the visceral surface toward the lumen onto a 2-mm polytetraflouroethylene (PTFE) graft. Graft patency was studied by in vivo Dopler. In vitro evaluations were done with hematoxylin-eosin stains, broadband cytokeratin staining, and monoclonal antibodies for macrophages. Explanation of the grafts was done in terminal operation at 7, 14, and 21 days. The MLG showed progressive fibroblastic proliferation in direct proportion to the age of the graft, but this did not lead to graft occlusion. However, a significant number of NLG were not patent at each time period studied. We concluded that mesothelial cell lining of smallcaliber PTFE grafts could enhance the short-term patency more than using the PTFE without the mesothelial lining. The use of such hybrid small-caliber grafts has a potential for improving the patency of these artificial vascular graft substitutes.
authors.affiliation: Department of Surgery, Division of Vascular Surgery, University of California Medical Center, San Diego 92103, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Sparks
authors.forename: Uttam
authors.initials: U
authors.lastname: Tripathy
authors.forename: Abraham
authors.initials: A
authors.lastname: Broudy
authors.forename: John J
authors.initials: JJ
authors.lastname: Bergan
authors.forename: Norman H
authors.initials: NH
authors.lastname: Kumins
authors.forename: Erik L
authors.initials: EL
authors.lastname: Owens
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D004727:Endothelium / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology; D004848:Epithelium / Q000473:pathology*; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D023421:Models, Animal; D010537:Peritoneum / Q000166:cytology*; D011138:Polytetrafluoroethylene / Q000009:adverse effects / Q000627:therapeutic use*; D011474:Prosthesis Design; D011817:Rabbits; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control*; D018608:Ultrasonography, Doppler
chemical_list: D011138:Polytetrafluoroethylene