Interleukin 1beta (IL-1beta), IL-10, tumor necrosis factor-alpha, and cellular proliferation index in peripheral blood mononuclear cells in patients with ankylosing spondylitis.
OBJECTIVE
To evaluate cytokine production and cellular proliferation index (CPI) in peripheral blood mononuclear cells (PBMC) of patients with ankylosing spondylitis (AS), and their association with clinical variables.


METHODS
In a cross sectional study we compared the production of tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and IL-10 and CPI in response to phytohemagglutinin (PHA) in PBMC of 27 patients with AS and 24 healthy controls. We also assessed clinical characteristics including disease activity index (BASDAI) and functional index (BASFI).


RESULTS
Levels of IL-1beta were higher in patients with AS (median 242 pg/ml) than in controls (median 65 pg/ml); p = 0.002. No differences were observed in median levels of TNF-alpha or IL-10 between AS and controls. Patients had a reduction in CPI (1.2 in AS vs 1.8 in controls; p < 0.001). A positive correlation was observed between IL-10 production and age (rho = 0.34, p = 0.01). A borderline negative correlation was observed between CPI and age (rho = -0.26, p = 0.07).


CONCLUSIONS
Patients with AS had high production of IL-1beta compared with controls and a poor response in CPI. These findings may explain the lack of response for microbial antigens mediated by the innate immune response.
Vazquez-Del Mercado
Monica
M
Instituto de Biología Molecular en Medicina, Universidad de Guadalajara, Jalisco, México. bestvme@standford.edu
Garcia-Gonzalez
Araceli
A
Muñoz-Valle
José Francisco
JF
Garcia-Iglesias
Trinidad
T
Martinez-Bonilla
Gloria
G
Bernard-Medina
Guislaine
G
Sanchez-Ortiz
Adriana
A
Ornelas-Aguirre
José M
JM
Salazar-Paramo
Mario
M
Gamez-Nava
Jorge I
JI
Gonzalez-Lopez
Laura
L
D000293:Adolescent; D000328:Adult; D002455:Cell Division / Q000276:immunology; D003430:Cross-Sectional Studies; D016207:Cytokines / Q000032:analysis*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000166:cytology* / Q000276:immunology; D008875:Middle Aged; D012720:Severity of Illness Index; D013167:Spondylitis, Ankylosing / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10