PCR amplification and polymorphism analysis of the intergenic spacer region of ribosomal DNA in Tuber borchii.
PCR amplification of the complete intergenic spacer region (IGS) of the Tuber borchii nuclear ribosomal repeat was obtained using universal primers CNL 12 and NS1rev. In order to improve amplification yield a specific primer, T1, was selected from a partial sequence of the IGS product. IGS diversity was characterized both at the intraindividual and intraspecific level. The results obtained at the intraindividual level showed 10% varying repeats on ten screened colonies, while at the intraspecific level the IGS polymorphism was evident as difference in length amplification between mycelial strains and fruit bodies: 3.5 kb and 2 kb respectively.
Ciarmela
Pasquapina
P
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, PU, Italy.
Potenza
Lucia
L
Cucchiarini
Luigi
L
Zeppa
Sabrina
S
Stocchi
Vilberto
V
D001203:Ascomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D025282:Mycelium / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer