Genome- and proteome-based technologies: status and applications in the postgenomic era.
Genes are transcribed to pre-mRNA, further processed to various mRNAs and then translated into proteins that may be post-translationally modified and subsequently function as the ultimate effecting molecules in the cell. Diagnostic options may be addressed as hybridization-based techniques to monitor nucleotide mutations or transcript levels. These techniques are highly suitable for high-throughput analyses based on DNA chip technology. They will enter the diagnostic practice as routine assays, although some obstacles must be addressed. Proteomics-based techniques are less suitable for high-throughput analyses at the moment, but are closer to the functional level. The combination of 2-dimensional polyacrylamide gel electrophoresis and mass spectrometry analyses make a strong couple that may enter as diagnostic applications once more automated.
Honoré
B
B
Department of Medical Biochemistry, Ole Worms Allé, Building 170, University of Aarhus, DK-8000 Aarhus C, Denmark. bh@biokemi.au.dk
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001709:Biotechnology / Q000639:trends; D015180:Electrophoresis, Gel, Two-Dimensional; D015894:Genome, Human; D006801:Humans; D013058:Mass Spectrometry; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D011506:Proteins / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature
D011506:Proteins; D012333:RNA, Messenger