Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis.
Selenate reductase from Thauera selenatis was crystallized using ammonium sulfate as a precipitant. Crystals of selenate reductase belong to the space group C2, with unit-cell parameters a = 116.9, b = 67.5, c = 186.7 A, beta = 90 degrees. Native data to 2.1 A resolution have been collected and a heavy-atom derivative has been identified following soaking of the crystals in a solution of trimethyl lead acetate.
Maher
Megan J
MJ
Department of Biochemistry, University of Sydney, Sydney, NSW 2006, Australia. m.maher@biochem.usyd.edu.au
Macy
Joan M
JM
D003460:Crystallization; D018360:Crystallography, X-Ray; D010088:Oxidoreductases / Q000737:chemistry*; D020628:Thauera / Q000737:chemistry*
D010088:Oxidoreductases; C078108:selenate reductase