title: Impact of fish oil enriched total parenteral nutrition on DNA synthesis, cytokine release and receptor expression by lymphocytes in the postoperative period.
abstract: A prospective randomized study on sixty patients was conducted to investigate the effects of a fish oil containing total parenteral nutrition (TPN) regimen in the postoperative period on lymphocyte subset distribution, proliferation, cytokine production and interleukin-2 receptor (IL-2R) expression. Patients who underwent large bowel surgery were divided into three groups. Nineteen patients received TPN with fish oil (0.2 g/kg body weight per day) plus soybean oil (1.0 g/kg per day), twenty patients received soybean oil (1.2 g/kg per day), and twenty-one patients who were on a fat-free regimen served as the control group. Natural killer (NK) cells, total, B-, T-, T4-, T8-lymphocytes, proliferation of lymphocytes, in vitro production of IL-2, IFN-gamma, TNF-alpha, and IL-2R expression were measured. Fish oil administration did not affect subset distribution and proliferation of lymphocytes. Production of interleukin-2 (IL-2), interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha) was augmented, and IL-2R expression less enhanced compared with the controls. It is concluded that administration of 0.2 g/kg per day fish oil after a moderate surgical stress is not immunosuppressive, but enhances the production of IFN-gamma, TNF-alpha and possibly IL-2.
authors.affiliation: Department of Medicine, University of Göttingen, Germany. schauder@med.uni-goettingen.de
authors.forename: P
authors.initials: P
authors.lastname: Schauder
authors.forename: U
authors.initials: U
authors.lastname: Röhn
authors.forename: G
authors.initials: G
authors.lastname: Schäfer
authors.forename: G
authors.initials: G
authors.lastname: Korff
authors.forename: H D
authors.initials: HD
authors.lastname: Schenk
mesh_terms: D000368:Aged; D016207:Cytokines / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D004311:Double-Blind Method; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D007420:Intestine, Large / Q000601:surgery*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011182:Postoperative Care / Q000379:methods*; D011446:Prospective Studies; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013024:Soybean Oil / Q000494:pharmacology; D013312:Stress, Physiological / Q000276:immunology
chemical_list: D016207:Cytokines; D005395:Fish Oils; D015375:Receptors, Interleukin-2; D013024:Soybean Oil; D004247:DNA