title: Identification of antigenically distinct populations of Leishmania (Viannia) guyanensis from Manaus, Brazil, using monoclonal antibodies.
abstract: Eighty Leishmania isolates from patients who contracted cutaneous leishmaniasis in the Manaus region, Amazonas State, Brazil, were identified as Leishmania (Viannia) guyanensis by the electrophoretic profiles of six enzymes. None reacted with the species-specific monoclonal antibody B19. Two L. (V.) guyanensis subpopulations were detected with the monoclonals B2 and B12. The lack of B19 expression by the L. (V.) guyanensis strains in the present study contrasts with that of the vast majority of the strains of the same parasite from eastern Amazonia and French Guyana that express the epitope.
authors.affiliation: Núcleo de Medicina Tropical, Universidade de Brasília, Campus Universitário, Asa Norte. Caixa Postal 04517, DF, 70919-970, Brasilia, Brazil. gromero@unb.br
authors.forename: Gustavo Adolfo Sierra
authors.initials: GA
authors.lastname: Romero
authors.forename: Edna
authors.initials: E
authors.lastname: Ishikawa
authors.forename: Elisa
authors.initials: E
authors.lastname: Cupolillo
authors.forename: Cristiane Bentin
authors.initials: CB
authors.lastname: Toaldo
authors.forename: Marcus Vinitius de Farias
authors.initials: MV
authors.lastname: Guerra
authors.forename: Marcilene Gomes
authors.initials: MG
authors.lastname: Paes
authors.forename: Vanize de Oliveira
authors.initials: Vde O
authors.lastname: Macêdo
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Shaw
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D001938:Brazil; D004586:Electrophoresis; D000939:Epitopes; D006801:Humans; D018334:Leishmania guyanensis / Q000145:classification* / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes