Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection.
The peptide snakin-2 (StSN2) has been isolated from potato (Solanum tuberosum cv Jaerla) tubers and found to be active (EC(50) = 1-20 microM) against fungal and bacterial plant pathogens. It causes a rapid aggregation of both Gram-positive and Gram-negative bacteria. The corresponding StSN2 cDNA encodes a signal sequence followed by a 15-residue acidic sequence that precedes the mature StSN2 peptide, which is basic (isoelectric point = 9.16) and 66 amino acid residues long (molecular weight of 7,025). The StSN2 gene is developmentally expressed in tubers, stems, flowers, shoot apex, and leaves, but not in roots, or stolons, and is locally up-regulated by wounding and by abscisic acid treatment. Expression of this gene is also up-regulated after infection of potato tubers with the compatible fungus Botritys cinerea and down-regulated by the virulent bacteria Ralstonia solanacearum and Erwinia chrysanthemi. These observations are congruent with the hypothesis that the StSN2 is a component of both constitutive and inducible defense barriers.
Berrocal-Lobo
Marta
M
Laboratorio de Bioquímica y Biología Molecular, Departamento de Biotecnología-Universidad Politecnica Madrid, Escuela Tecnica Superior Ingenieros Agrónomos, E-28040 Madrid, Spain.
Segura
Ana
A
Moreno
Manuel
M
López
Gemma
G
García-Olmedo
Francisco
F
Molina
Antonio
A
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D001483:Base Sequence; D023082:Defensins; D005658:Fungi / Q000187:drug effects / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018514:Plant Structures / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology; D013314:Stress, Mechanical
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D010940:Plant Proteins; C117071:SN1 protein, Solanum tuberosum; D000040:Abscisic Acid