Protein electrophoretic mobility shift assay to monitor redox state of thioredoxin in cells.
Bersani
Neil A
NA
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331, USA.
Merwin
Jason R
JR
Lopez
Nathan I
NI
Pearson
George D
GD
Merrill
Gary F
GF
D000478:Alkylation; D002460:Cell Line; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D017353:Gene Deletion; D005800:Genes, Fungal; D006801:Humans; D015151:Immunoblotting; D007460:Iodoacetamide; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry* / Q000378:metabolism*
D013879:Thioredoxins; D013880:Thioredoxin-Disulfide Reductase; D004229:Dithiothreitol; D007460:Iodoacetamide