Delayed abscission and shorter Internodes correlate with a reduction in the ethylene receptor LeETR1 transcript in transgenic tomato.
Stable transformation of tomato (Lycopersicon esculentum cv Ailsa Craig) plants with a construct containing the antisense sequence for the receiver domain and 3'-untranslated portion of the tomato ethylene receptor (LeETR1) under the control of an enhanced cauliflower mosaic virus 35S promoter resulted in some expected and unexpected phenotypes. In addition to reduced LeETR1 transcript levels, the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduced plant size. Fruit coloration and softening were essentially unaffected, and all the seedlings from first generation seed displayed a normal triple response to ethylene. Two independent lines with a single copy of the transgene and reduced LeETR1 transcript accumulation were selected for detailed phenotypic analysis of second generation (R1) plants. Delayed abscission, shorter internode length, and reduced auxin movement all correlated with the presence of the transgene and the degree of reduced LeETR1 transcript accumulation. No significant differences were noted for fruit coloration or fruit softening on R1 plants and all seedlings from R1 and R2 seed displayed a normal triple response. LeETR2 transcript accumulation was only slightly reduced in the R1 plants compared with azygous plants, and LeETR3 (NR) transcript levels appeared to be unaffected by the transgene. We propose that ethylene signal transduction occurs through parallel paths that partially intersect to regulate shared ethylene responses.
Whitelaw
Catherine A
CA
Soybean Genomics Improvement Laboratory, United States Department of Agriculture, Building 006, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA.
Lyssenko
Nicholas N
NN
Chen
Liwei
L
Zhou
Dingbo
D
Mattoo
Autar K
AK
Tucker
Mark L
ML
D001692:Biological Transport; D003433:Crosses, Genetic; D005030:Ethylenes / Q000494:pharmacology; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D007210:Indoleacetic Acids / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics*; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics*; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic
D005030:Ethylenes; D007210:Indoleacetic Acids; D010940:Plant Proteins; D018749:RNA, Plant; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; C030737:indoleacetic acid; C036216:ethylene