title: Association of Epstein-Barr virus and nasopharyngeal carcinoma in Caucasian patients.
abstract: We evaluated the association of nasopharyngeal carcinoma (NPC) and Epstein-Barr virus (EBV) in Spanish patients, and studied the expression of EBV products (latent membrane protein-1 [LMP-1] and ZEBRA proteins) by NPC cells and its possible prognostic value. In situ hybridization (ISH) for EBV-encoded nonpolyadenylated RNAs (EBERs) and immunohistochemical expression of LMP-1 and ZEBRA proteins by immunohistochemistry were examined in formalin-fixed, paraffin-embedded NPC specimens from 30 patients, and a survival analysis was done by the Kaplan-Meier method. We detected EBERs by ISH in 96.67% of the NPC cases, and detected expression of LMP-I in 43.33% of the NPC cases and expression of ZEBRA protein in 6.67% of the NPC cases. We conclude that ISH for expression of EBERs is an adequate method for detection of EBV in NPC. LMP-1 is not frequently expressed in NPC cells (43.33%). Most NPC cells carry a latent EBV infection. LMP-1 expression might have worsened the prognosis of NPC in our series.
authors.affiliation: Otolaryngology Department, Ramón y Cajal Hospital, University of Alcalá, Madrid, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Plaza
authors.forename: Ana I
authors.initials: AI
authors.lastname: Manzanal
authors.forename: Luis
authors.initials: L
authors.lastname: Fogué
authors.forename: Almudena
authors.initials: A
authors.lastname: Santón
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Martínez-Montero
authors.forename: Carmen
authors.initials: C
authors.lastname: Bellas
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000368:Aged; D000369:Aged, 80 and over; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000175:diagnosis; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology / Q000821:virology*; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D012269:Ribosomal Proteins; D013030:Spain / Q000453:epidemiology; D016019:Survival Analysis; D015534:Trans-Activators / Q000032:analysis / Q000235:genetics; D014763:Viral Matrix Proteins / Q000032:analysis / Q000235:genetics; D014764:Viral Proteins; D044465:White People / Q000235:genetics*
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D015534:Trans-Activators; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C067169:RPL22 protein, human