Effect of exercise training on ANP receptors.
To clarify whether exercise-training affects ANP function, we trained male Wistar rats by treadmill running for nine weeks and measured ANP receptor number and affinity in the kidney, lung and adrenal. We also measured guanylate cyclase activity, by which second messenger cGMP accumulates. The number of adrenal ANP receptor significantly increased after exercise-training. There was no significant difference of affinity for all the organs examined between the training group and the control group. Guanylate cyclase activity tended to decrease in the kidney in the training group. A significant difference was found when the samples were stimulated by C-type natriuretic peptide (CNP). There was no significant difference in guanylate cyclase activity in the lung and adrenal. These findings are consistent with the exercise-induced hypervolemia, but not with the anti-hypertensive role of exercise-training.
Suda
K
K
Department of Human System Science, Tokyo Institute of Technology, Japan. Suda@taiiku.titech.ac.jp
Hagiwara
H
H
Kotani
Y
Y
Kato
K
K
Sasaki
M
M
Izawa
T
T
Komabayashi
T
T
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology; D010805:Physical Conditioning, Animal / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D015290:Second Messenger Systems
D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP