[Effects of medium and culture conditions on polysaccharide synthesis by suspension cell culture of Gardenia jasminoides Eills].
The effects of medium and culture condition on polysaccharide synthesis by suspension culture of Gardenia jasminoides Eills were studied. The results show that B5 was the optimum medium, 5-10 days was suitable for subculture periods, and an inoculum of 80 g/L wet cell was better to cell growth and polysaccharide accumulation. For carbon source, it was better to use glucose than sucrose on cell growth, but due to the higher price of glucose, 45 g/L compound carbon source combined sucrose with glucose (1:1) was the optimum. The effect of different kinds of nitrogen source on cell growth and polysaccharide synthesis was not so big, and 40-50 mmol/L nitrogen was optimum, lower concentration of nitrogen source could inhibit the synthesis of polysaccharides. In addition, by controlling the harvest time of the polysaccharide in the suspension culture, the accumulation of yellow pigment could be prevented, which is easier to the polysaccharide purification.
Wang
G L
GL
Biology Science College of Liaoning Normal University, Dalian 116029, China. guanlinwang@163.com
Shi
R F
RF
Fang
H J
HJ
D002452:Cell Count / Q000379:methods; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D011134:Polysaccharides / Q000096:biosynthesis*; D019666:Rubiaceae / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D011134:Polysaccharides; D013395:Sucrose; D005947:Glucose; D009584:Nitrogen