title: Analysis of the role of RecQ helicases in RNAi in mammals.
abstract: The identity of mammalian genes involved in RNA interference (RNAi), the targeted sequence-specific mRNA degradation by double-stranded RNA (dsRNA), is poorly defined. Here we report the analysis of mice with null mutations of Wrn, Blm, and RecQ1 genes that are related to Mut-7 and Qde3, two genes essential for RNAi in Caenorhabditis elegans and quelling in Neurospora, respectively. Our results suggest that Wrn, Blm, and RecQ1 are not involved in sequence-specific mRNA degradation in mammals in response to dsRNA, suggesting potential differences in the mammalian RNAi pathway.
authors.affiliation: Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104-6018, USA.
authors.forename: Paula
authors.initials: P
authors.lastname: Stein
authors.forename: Petr
authors.initials: P
authors.lastname: Svoboda
authors.forename: Deborah J
authors.initials: DJ
authors.lastname: Stumpo
authors.forename: Perry J
authors.initials: PJ
authors.lastname: Blackshear
authors.forename: David B
authors.initials: DB
authors.lastname: Lombard
authors.forename: Brad
authors.initials: B
authors.lastname: Johnson
authors.forename: Richard M
authors.initials: RM
authors.lastname: Schultz
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005090:Exodeoxyribonucleases; D005656:Fungal Proteins; D051379:Mice; D009491:Neurospora / Q000235:genetics; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D053484:RecQ Helicases; D000071657:Werner Syndrome Helicase
chemical_list: D005656:Fungal Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D005090:Exodeoxyribonucleases; D000251:Adenosine Triphosphatases; C097151:Bloom syndrome protein; C506725:RECQL protein, human; D004265:DNA Helicases; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase; C402647:QDE-3 protein, Neurospora crassa