title: The projection domain of MAP2b regulates microtubule protrusion and process formation in Sf9 cells.
abstract: The expression of microtubule-associated protein 2 (MAP2), developmentally regulated by alternative splicing, coincides with neurite outgrowth. MAP2 proteins contain a microtubule-binding domain (C-terminal) that promotes microtubule assembly and a poorly characterized domain, the projection domain (N-terminal), extending at the surface of microtubules. MAP2b differs from MAP2c by an additional sequence of 1372 amino acids in the projection domain. In this study, we examined the role of the projection domain in the protrusion of microtubules from the cell surface and the subsequent process formation in Sf9 cells. In this system, MAP2b has a lower capacity to induce process formation than MAP2c. To investigate the role of the projection domain in this event, we expressed truncated forms of MAP2b and MAP2c that have partial or complete deletion of their projection domain in Sf9 cells. Our results indicate that process formation is induced by the microtubule-binding domain of these MAP2 proteins and is regulated by their projection domain. Furthermore, the microtubule-binding activity of MAP2b and MAP2c truncated forms as well as the structural properties of the microtubule bundles induced by them do not seem to be the only determinants that control the protrusion of microtubules from the cell surface in Sf9 cells. Rather, our data suggest that microtubule protrusion and process formation are regulated by intramolecular interactions between the projection domain and its microtubule-binding domain in MAP2b.
authors.affiliation: Département de pathologie et biologie cellulaire, Université de Montréal, Montréal, Québec, Canada, H3T 1J8.
authors.forename: Dave
authors.initials: D
authors.lastname: Bélanger
authors.forename: Carole Abi
authors.initials: CA
authors.lastname: Farah
authors.forename: Minh Dang
authors.initials: MD
authors.lastname: Nguyen
authors.forename: Michel
authors.initials: M
authors.lastname: Lauzon
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cornibert
authors.forename: Nicole
authors.initials: N
authors.lastname: Leclerc
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D005822:Genetic Vectors; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D017434:Protein Structure, Tertiary; D018411:Spodoptera / Q000235:genetics*; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D008869:Microtubule-Associated Proteins; D014404:Tubulin