title: Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD).
abstract: AFLP profiles characteristic to Panax ginseng and Panax quinquefolius were generated using primers E-AGG/M-CAA. P. ginseng samples from different farms in China and Korea are homogeneous genetically [similarity index (SI) = 0.88-0.99], whereas samples of P. quinquefolius from different sources are much more heterogeneous (SI = 0.64-0.96). Detailed analysis of one of the polymorphic bands in P. ginseng led to the identification of a minisatellite Pg2, which contains eight repeats of 5'-AGGACTCATCACATTGTTACTC. The minisatellite DNA was consequently used in directed amplification minisatellite region DNA analysis to authenticate the two ginsengs.
authors.affiliation: Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Ha
authors.forename: P C
authors.initials: PC
authors.lastname: Shaw
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Forrest C F
authors.initials: FC
authors.lastname: Yau
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis*; D004365:Drugs, Chinese Herbal; D005784:Gene Amplification; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D005894:Panax / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011786:Quality Control
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal