Up-regulation of telomerase activity in Herpesvirus saimiri immortalized human T-lymphocytes.
Human T-lymphocytes can be transformed to unlimited growth by Herpesvirus saimiri (HVS). We studied the telomerase activity of a recently established HVS immortalized human CD4 T cell clone in comparison to peripheral blood lymphocytes (PBL) and unstimulated or phytohemagglutinin (PHA)-stimulated CD4 T-cells by a Telomeric Repeat Amplification-Protocol (TRAP) -Assay. Telomerase activity in PHA-stimulated CD4 T-cells was seven-fold and in HVS-infected CD4 T-cells 14-fold higher than in untreated CD4 T-cells. The HVS immortalized T-cell clone provides a useful tool for studying the regulation of telomerase activity during carcinogenesis and for testing of telomerase-inhibitory drugs.
Harnack
U
U
Humboldt-University Berlin, AG Molecular Gene- and Immunotherapy, Germany.
Lehmann
C
C
Matthes
E
E
Pecher
G
G
D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000201:enzymology* / Q000821:virology; D002472:Cell Transformation, Viral / Q000502:physiology*; D018922:HL-60 Cells / Q000166:cytology / Q000201:enzymology; D006567:Herpesvirus 2, Saimiriine / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000201:enzymology; D019098:Telomerase / Q000378:metabolism*; D015854:Up-Regulation
D019098:Telomerase