Oxidative metabolite of dopamine, 3,4-dihydroxyphenylacetaldehyde, induces dopamine release from PC12 cells by a Ca2+-independent mechanism.
3,4-dihydroxyphenylacetaldehyde (DOPALD), an oxidative metabolite of dopamine (DA), induced dose-dependent DA release from pheochromocytoma (PC12) cells without affecting leakage of lactate dehydrogenase from the cells. DOPALD-induced DA release was independent of extracellular Ca2+ concentration and was not blocked by nifedipine, an L-type Ca2+ channel antagonist. These results indicated a novel intrinsic role of DOPALD in dopaminergic nerve terminals that may take part in the activation of dopamine neurons.
Hashimoto
Tsuneichi
T
Department of Pharmacology, Kyoto Prefecutural University of Medicine, Kawaramachi-Hirokoji, Kamikyoku, 602-8566, Kyoto, Japan.
Yabe-Nishimura
Chihiro
C
D015102:3,4-Dihydroxyphenylacetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology; D010084:Oxidation-Reduction; D016716:PC12 Cells; D010300:Parkinson Disease / Q000378:metabolism; D051381:Rats
D002121:Calcium Channel Blockers; D015102:3,4-Dihydroxyphenylacetic Acid; C007430:3,4-dihydroxyphenylacetaldehyde; D007770:L-Lactate Dehydrogenase; D009543:Nifedipine; D002118:Calcium; D004298:Dopamine