Allergenicity and immunochemical characterization of six varieties of Olea europaea.
BACKGROUND
The inhalation of Olea europaea pollen is one of the most important causes of allergic respiratory diseases in the Mediterranean basin. The objective of this study was to investigate the antigenic and allergenic composition of six different O. europaea varieties collected in southern Spain.


METHODS
The varieties included in the study were: Acebuche (wild olive), Carrasqueño, Nevado, Hojiblanco, Manzanillo and Picual. Extracts of these six varieties were prepared. Twenty-nine olive individuals with an immunoglobulin(Ig)E-mediated allergy to olive pollen were skin tested with these extracts. The antigenic profile of these extracts was evaluated by SDS-PAGE; the allergenic profile was investigated by immunoblotting using the serum of these 29 individuals. The Ole e 1 content was established by ELISA inhibition using purified Ole e 1 and rabbit polyclonal antibodies and by scanning densitometry.


RESULTS
The extracts that induced the smallest wheal size were Acebuche and Hojiblanco, being significantly different from the rest of the extracts. The antigenic and allergenic profiles of the extracts also varied. The Ole e 1 content ranged from 0.050 in Hojiblanco to 0.232 in Manzanillo, measured by ELISA inhibition and from 0.153 in Hojiblanco to 0.677 in Nevado, measured by scanning densitometry.


CONCLUSIONS
The different varieties of O. europaea pollen studied demonstrated great differences in the in vivo and in vitro potency of the extracts. There were significant differences in the Ole e 1 content, while the protein content remained very similar in these extracts. This study confirms previous observations of a great variability in the antigenic and allergenic composition of O. europaea pollen extracts and establishes significant differences in Ole e 1 content.
Carnés Sánchez
J
J
C.B.F. LETI, S.A. Research & Development Laboratories, Madrid, Spain.
Iraola
V M
VM
Sastre
J
J
Florido
F
F
Boluda
L
L
Fernández-Caldas
E
E
D000485:Allergens / Q000097:blood* / Q000145:classification / Q000276:immunology*; D000941:Antigens / Q000097:blood / Q000145:classification / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010936:Plant Extracts / Q000276:immunology*; D010940:Plant Proteins / Q000097:blood / Q000145:classification / Q000276:immunology; D011058:Pollen / Q000145:classification / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000276:immunology; D013030:Spain / Q000453:epidemiology; D014197:Trees / Q000145:classification / Q000276:immunology
D000485:Allergens; D000941:Antigens; D010936:Plant Extracts; D010940:Plant Proteins