Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes.
The functional properties of the three most widely distributed alpha subunit isoforms of the Na,K-ATPase are not well known, particularly concerning the voltage dependence of their activity and cation binding kinetics. We measured the electrogenic activity generated by Na,K-ATPases resulting from co-expression of the rat alpha1, alpha2* or alpha3* subunits with the rat beta1 subunit in Xenopus oocytes; alpha2* and alpha3* are ouabain-resistant mutants of the alpha2 and alpha3 isoform, which allowed selective inhibition of the endogenous Na(+),K(+)-pump of the oocyte. In oocytes expressing the three isoforms of the alpha subunit, K(+) induced robust outward currents that were largely ouabain-sensitive. In addition, ouabain-sensitive inward currents were recorded for all three isoforms in sodium-free and potassium-free acid solutions. The very similar voltage dependence of the Na(+),K(+)-pump activity observed in the absence of extracellular Na(+) indicated a similar stoichiometry of the transported cations by the three isoforms. The affinity for extracellular K(+) was slightly lower for the alpha2* and alpha3* than for the alpha1 isoform. The alpha2* isoform was, however, more sensitive to voltage-dependent inhibition by extracellular Na(+), indicating a higher affinity of the extracellular Na(+) site in this isoform. We measured and controlled [Na(+)](i) using a co-expressed amiloride-sensitive Na(+) channel. The intracellular affinity for Na(+) was slightly higher in the alpha2* than in the alpha1 or alpha3* isoforms. These results suggest that the alpha2 isoform could have an activity that is strongly dependent upon [Na(+)](o) and [K(+)](o). These concentrations could selectively modulate its activity when large variations are present, for instance in the narrow intercellular spaces of brain or muscle tissues.
Horisberger
Jean-Daniel
JD
Institut de Pharmacologie et de Toxicologie, rue du Bugnon 27, CH-1005 Lausanne, Switzerland. jean-daniel.horisberger@ipharm.unil.ch
Kharoubi-Hess
Solange
S
D000818:Animals; D001667:Binding, Competitive; D004553:Electric Conductivity; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D009865:Oocytes; D011188:Potassium / Q000378:metabolism; D051381:Rats / Q000378:metabolism*; D012964:Sodium / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism* / Q000502:physiology; D014982:Xenopus laevis
D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium