Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements.
Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific cDNAs encoding barley proteins selected because of their significance in sieve tube function. mRNA encoding the H(+)/sucrose co-transporter SUT1, a putative aquaporin and the H(+)/ATPase PPA1 were detected in sieve tube sap. These mRNA species appear to be present at very low concentrations. mRNA encoding the potassium transporter HAK1 could not be detected. The results strongly suggest that some mRNA species are imported into sieve elements, which are enucleate, from neighbouring companion cells.
Doering-Saad
Christian
C
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Newbury
H John
HJ
Bale
Jeff S
JS
Pritchard
Jeremy
J
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001042:Aphids / Q000737:chemistry; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020346:Aquaporins; D027682:Cation Transport Proteins; C110543:HAK1 protein, plant; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C078729:sucrose transport protein, plant; D000251:Adenosine Triphosphatases