Cdc25C interacts with PCNA at G2/M transition.
Cdc25 activates maturation promoting factor (MPF) and promotes mitosis by removing the inhibitory phosphate from the Tyr-15 of Cdc2 in human cells. In this study, we searched the interacting protein(s) of human Cdc25C using the yeast two-hybrid screen and identified proliferating cell nuclear antigen (PCNA) as an interacting partner of Cdc25C. The interaction between Cdc25C and PCNA was confirmed in vitro and in vivo. Co-immunoprecipitation analyses using human T cell line, Jurkat, further revealed that Cdc25C interacted with PCNA transiently when cells began to enter mitosis. Immunofluorescence analysis also showed that Cdc25C and PCNA were transiently co-localized in the nucleus at the beginning of M phase. Together with the previous observations of the interaction between various cdc/cyclin and PCNA, our findings strongly suggested a potential role of PCNA at the G2 to M phase transition of cell cycle.
Kawabe
Takumi
T
Department of Molecular Genetics, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan.
Suganuma
Masashi
M
Ando
Tomoaki
T
Kimura
Mayumi
M
Hori
Haruna
H
Okamoto
Takashi
T
D018797:Cell Cycle Proteins / Q000032:analysis / Q000502:physiology*; D016195:G2 Phase; D006801:Humans; D019169:Jurkat Cells; D008938:Mitosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D020687:cdc25 Phosphatases / Q000032:analysis / Q000502:physiology*
D018797:Cell Cycle Proteins; D018809:Proliferating Cell Nuclear Antigen; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases