Experimental studies on synergism between aminoglycosides and the antimicrobial antiinflammatory agent diclofenac sodium.
The antiinflammatory agent diclofenac sodium (Dc) exhibited remarkable antibacterial effects both in vitro and in vivo. Fifteen different bacteria sensitive to Dc as well as to a number of common antibiotics were tested for synergistic effects in vitro. Disc diffusion test with Dc and aminoglycosides assessed by stringent computation showed clear-cut synergism. Synergism between Dc and streptomycin (Sm) was found to be statistically significant (p < or = 0.01) when compared with their individual effects. By the checkerboard assessment procedure, the fractional inhibitory concentration (FIC) index of this combination was found to be 0.49, confirming synergism. The mouse protective capacity of this combination was then evaluated in vivo against S. typhimurium as the virulent infecting bacterium, and the size of bacterial load determined from infected autopsied animals. Statistical analysis by Student's 't' test suggested this drug combination is highly synergistic; synergism was also noted between Dc and other aminoglycosides.
Annadurai
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S
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Guha-Thakurta
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A
Sa
B
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Dastidar
S G
SG
Ray
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Chakrabarty
A N
AN
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D004008:Diclofenac / Q000494:pharmacology*; D004357:Drug Synergism; D051379:Mice; D008826:Microbial Sensitivity Tests; D012481:Salmonella Infections, Animal / Q000188:drug therapy
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004008:Diclofenac