title: Cutting edge: analysis of human V alpha 24+CD8+ NK T cells activated by alpha-galactosylceramide-pulsed monocyte-derived dendritic cells.
abstract: Human Valpha24(+) NKT cells constitute a counterpart of mouse Valpha14(+) NKT cells, both of which use an invariant TCR-alpha chain. The human Valpha24(+) NKT cells as well as mouse Valpha14(+) NKT cells are activated by glycolipids in a CD1d-restricted manner and produce many immunomodulatory cytokines, possibly affecting the immune balance. In mice, it has been considered from extensive investigations that Valpha14(+)CD8(+) NKT cells that express invariant TCR do not exist. Here we introduce human Valpha24(+)CD8(+) NKT cells. These cells share important features of Valpha24(+) NKT cells in common, but in contrast to CD4(-)CD8(-) (double-negative) or CD4(+) Valpha24(+) NKT cells, they do not produce IL-4. Our discovery may extend and deepen the research field of Valpha24(+) NKT cells as well as help to understand the mechanism of the immune balance-related diseases.
authors.affiliation: Department of Hematology, University of Tokyo, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Chiba
authors.forename: Mie
authors.initials: M
authors.lastname: Nieda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Azuma
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ishihara
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shibata
authors.forename: Takeo
authors.initials: T
authors.lastname: Juji
authors.forename: Hisamaru
authors.initials: H
authors.lastname: Hirai
mesh_terms: D018949:Antigens, CD1 / Q000276:immunology; D055610:Antigens, CD1d; D016827:CD8 Antigens / Q000032:analysis / Q000096:biosynthesis*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005699:Galactosylceramides / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D020298:U937 Cells
chemical_list: D018949:Antigens, CD1; D055610:Antigens, CD1d; C526877:CD1D protein, human; D016827:CD8 Antigens; D016207:Cytokines; D005699:Galactosylceramides; D016693:Receptors, Antigen, T-Cell, alpha-beta