title: PCR amplification and polymorphism analysis of the intergenic spacer region of ribosomal DNA in Tuber borchii.
abstract: PCR amplification of the complete intergenic spacer region (IGS) of the Tuber borchii nuclear ribosomal repeat was obtained using universal primers CNL 12 and NS1rev. In order to improve amplification yield a specific primer, T1, was selected from a partial sequence of the IGS product. IGS diversity was characterized both at the intraindividual and intraspecific level. The results obtained at the intraindividual level showed 10% varying repeats on ten screened colonies, while at the intraspecific level the IGS polymorphism was evident as difference in length amplification between mycelial strains and fruit bodies: 3.5 kb and 2 kb respectively.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, PU, Italy.
authors.forename: Pasquapina
authors.initials: P
authors.lastname: Ciarmela
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Luigi
authors.initials: L
authors.lastname: Cucchiarini
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis*; D025282:Mycelium / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer