title: Studies of the ceruloplasmin-lactoferrin complex.
abstract: We have previously shown that iron-containing human lactoferrin (LF) purified from breast milk is able to form both in vitro and in vivo a complex with ceruloplasmin (CP), the copper-containing protein of human plasma. Here we present evidence that the CP-LF complex is dissociated by high concentrations of NaCl, CaCl2, or EDTA, or by decreasing the pH to 4.7. In addition, DNA, bacterial lipopolysaccharide, and heparin can displace CP from its complex with LF. Antibodies to either of the two proteins also cause dissociation of the complex.
authors.affiliation: Institute for Experimental Medicine, Saint-Petersburg, Russia. molgen.@molgen.iem.ras.spb.ru
authors.forename: Maria O
authors.initials: MO
authors.lastname: Pulina
authors.forename: Elena T
authors.initials: ET
authors.lastname: Zakharova
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Sokolov
authors.forename: Mikhail M
authors.initials: MM
authors.lastname: Shavlovski
authors.forename: Mikhail G
authors.initials: MG
authors.lastname: Bass
authors.forename: Kirill V
authors.initials: KV
authors.lastname: Solovyov
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Kokryakov
authors.forename: Vadim B
authors.initials: VB
authors.lastname: Vasilyev
mesh_terms: D002122:Calcium Chloride / Q000494:pharmacology; D002570:Ceruloplasmin / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin / Q000378:metabolism*; D046911:Macromolecular Substances; D008895:Milk, Human / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D046911:Macromolecular Substances; D012965:Sodium Chloride; D004492:Edetic Acid; D002570:Ceruloplasmin; D007781:Lactoferrin; D002122:Calcium Chloride