Enzyme-based sensor arrays for rapid characterization of complex disaccharide solutions.
An enzyme-based sensor array has been developed to detect multiple disaccharides in aqueous solutions. Porous agarose beads, derivatized with enzymes for assaying disaccharides, are localized within wells etched into a silicon chip in a regular 5 x 7 array. Each well is individually addressable and acts as a microanalysis chamber where sample solution passes through the agarose matrix and is exposed to the enzymes. Detection is achieved by observing the increase in absorbance of a quinoneimine dye produced during the reaction. This technique is used to quantify the disaccharides lactose, sucrose, and maltose and the monosaccharide glucose. Preexisting glucose in the sample complicates multicomponent sensing but can be accounted for by including a glucose sensor in the array. This detection strategy is applied to the simultaneous analysis of these sugars in several beverages.
Curey
Theodore E
TE
Department of Chemistry and Biochemistry, Institute for Cellular & Molecular Biology, Center for Nano and Molecular Science and Technology, University of Texas at Austin, Mail Code A5300, Austin, TX 78712, USA.
Salazar
Mary Alice
MA
Oliveira
Philip
P
Javier
James
J
Dennis
Patty J
PJ
Rao
Poornima
P
Shear
Jason B
JB
D001628:Beverages / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D004187:Disaccharides / Q000032:analysis*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007785:Lactose / Q000032:analysis; D008320:Maltose / Q000032:analysis; D013395:Sucrose / Q000032:analysis
D004187:Disaccharides; D013395:Sucrose; D008320:Maltose; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose; D007785:Lactose