Uterus-innervating neurones of paracervical ganglion in the pig: immunohistochemical characteristics.
Immunohistochemical characteristics of neurones innervating the porcine uterus located in paracervical ganglia were studied with a combination of retrograde fluorescent tracing and immunofluorescence. Retrograde fluorescent tracer Fast Blue (FB) was injected into the uterine horn and uterine cervix. The presence of biologically active substances, tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), galanin (GAL), Met-enkephalin-Arg-Gly-Leu (MEAGL) and calcitonin gene-related peptide (CGRP) was studied in FB-positive neurones localised in paracervical ganglia. FB-positive neurones containing TH, NPY, VIP and MEAGL were numerous, while those containing CGRP were scarce. The results pointed to some species-related differences in immunohistochemical coding of neurones of paracervical ganglion responsible for uterus innervation.
Wasowicz
Krzysztof
K
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Olsztyn-Kortowo II, Poland. wasowicz@moskit.uwm.edu.pl
Podlasz
Piotr
P
Czaja
Krzysztof
K
Łakomy
Miroslaw
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D019004:Galanin / Q000032:analysis; D007001:Hypogastric Plexus / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology; D009478:Neuropeptide Y / Q000032:analysis; D013552:Swine; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014599:Uterus / Q000294:innervation*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide; D004744:Enkephalin, Methionine; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-; D019004:Galanin; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide