Growth-associated production of poly-3-hydroxybutyrate by Bacillus mycoides.
Bacillus mycoides strain RIJ B-017, a growth-associated poly-3-hydroxybutyrate (PHB) producer was grown on sucrose-containing media. PHB accumulated in cells up to 72% of dry cell mass. The overall maximum value of PHB yield (Yp/s) and productivities (Qp and qp) 250 mgp/gs, 120 mgp L-1 h-1 and 30 mgp gx-1 h-1, respectively, were obtained at 15 g/L sucrose. Differential scanning calorimeter heating curve showed two peaks, one at 95.9 degrees C and another at 165.4 degrees C with a shoulder around 154.6 degrees C. The viscosity-average molar mass in chloroform at 27 degrees C was 505 kDa. The carbon content of PHB was 55.4% of the mass.
Thakur
P S
PS
Biochemistry Division, Regional Research Laboratory, Jorhat-785 006, Assam, India.
Borah
B
B
Baruah
S D
SD
Nigam
J N
JN
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D002244:Carbon / Q000032:analysis; D003470:Culture Media; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose; D000094122:Polyhydroxybutyrates
D002244:Carbon; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; D013395:Sucrose; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates