title: p53 protein accumulation in multiple oesophageal squamous cell carcinoma: relationship to risk factors.
abstract: To clarify mechanisms involved in the carcinogenesis of multiple oesophageal squamous cell carcinoma, the expression of p53 protein in 46 lesions surgically excised from 13 Japanese patients was investigated immunohistochemically and the relation of p53 protein accumulation to the patient's history of alcohol consumption and cigarette smoking was analyzed. p53 protein accumulation was observed in 13 main lesions, that is in 6 (85.7%) of 7 subjects with a history of heavy drinking and smoking, but only in 1 (16.7%) of 6 with no such history (Fisher's exact test, p = 0.025). As regards the 46 lesions, p53 protein accumulation was evident in 22 (88.0%) of 25 lesions of the high-risk patients, but in 7 (33.3%) of 21 lesions of the other subjects (Fisher's exact test, p < 0.001). p53 protein accumulation was similarly recognized in all oesophageal lesions in 5 of 7 high-risk patients. Thus, use of both alcohol and cigarettes is clearly associated with a high frequency of p53 protein accumulation in multiple oesophageal squamous cell carcinoma present at the same time. These findings are considered to support the concept of field carcinogenesis of the oesophagus.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. hsaeki@med.kyushu-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saeki
authors.forename: Shinji
authors.initials: S
authors.lastname: Ohno
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Koshi
authors.initials: K
authors.lastname: Araki
authors.forename: Akinori
authors.initials: A
authors.lastname: Egashira
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Watanabe
authors.forename: Masaru
authors.initials: M
authors.lastname: Morita
authors.forename: Keizo
authors.initials: K
authors.lastname: Sugimachi
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000009:adverse effects*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000209:etiology / Q000473:pathology; D004938:Esophageal Neoplasms / Q000737:chemistry* / Q000209:etiology / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009378:Neoplasms, Multiple Primary / Q000737:chemistry* / Q000209:etiology / Q000473:pathology; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53