Purification and crystallization of the yeast elongation factor eEF2.
Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.
Jørgensen
René
R
Institute of Molecular and Structural Biology, University of Aarhus, Gustav Wieds Vej 10C, DK-8000 Aarhus, Denmark.
Carr-Schmid
Anne
A
Ortiz
Pedro A
PA
Kinzy
Terri Goss
TG
Andersen
Gregers Rom
GR
D003460:Crystallization; D018360:Crystallography, X-Ray; D006639:Histidine / Q000737:chemistry; D009154:Mutation; D020652:Peptide Elongation Factor 2 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D020652:Peptide Elongation Factor 2; D006639:Histidine