[Technique of enzyme digestion adding brushing for isolating bronchial epithelial cells].
This study improved the previous techniques of harvesting rabbit bronchial epithelial cells. With 0.05% of trypsin on the epithelial side of trachea and bronchus, a mild digestion was used before a brushing protocol. The cells were identified with immunocytochemistry and electron microscope, both the cell viability and the membrane integrity of cells were evaluated. A comparison analysis of this method with the simple mechanic brushing and the simple digestion method were done. The results showed that, with this method, cells in a high purity and high viability could be obtained and the recovered cell number could be enough for experiments. It can be concluded that the method is useful and effective for isolating bronchial epithelial cells especially for small animals.
Qin
X
X
Department of Physiology, Hunan Medical University, Changsha 410078.
Sun
X
X
Zhang
C
C
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000648:ultrastructure*; D005260:Female; D008297:Male; D011817:Rabbits; D014357:Trypsin
D014357:Trypsin