Cell proliferation and lipid formation in hamster sebaceous gland cells.
BACKGROUND
The development of sebocytes and lipogenesis are known to be dependent on androgens. However, it is not fully understood whether growth factors are involved in the regulation of cell proliferation and lipid formation in sebaceous glands.


OBJECTIVE
This study was designed to evaluate the effect of growth factors such as epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF) on cell proliferation and lipogenesis in cultured hamster sebocytes.


METHODS
Cell proliferation and intracellular lipid accumulation in these hamster sebocytes treated with growth factors were examined.


RESULTS
EGF, TGF-alpha and bFGF augmented the proliferation of hamster sebocytes for 8 days in a time- and dose-dependent manner. However, KGF had no effect on their proliferation. Moreover, the accumulation of intracellular lipids consisting mainly of triglycerides was suppressed in EGF-, TGF-alpha-, bFGF- and KGF-treated hamster sebocytes.


CONCLUSIONS
EGF, TGF-alpha and bFGF, but not KGF, have mitogenic activity on hamster sebocytes, and all these growth factors act as antilipogenic factors. Moreover, it is likely that the formation of intracellular lipid droplets is independent of cell proliferation in hamster sebocytes.
Akimoto
N
N
Department of Biochemistry, Tokyo University of Pharmacy and Life Science, School of Pharmacy, Japan.
Sato
T
T
Sakiguchi
T
T
Kitamura
K
K
Kohno
Y
Y
Ito
A
A
D000818:Animals; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D012627:Sebaceous Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D006133:Growth Substances; D016211:Transforming Growth Factor alpha; D016222:Fibroblast Growth Factor 2; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor