In vitro selection of RNA aptamers that bind to cell adhesion receptors of Trypanosoma cruzi and inhibit cell invasion.
Trypanosoma cruzi causing Chagas' disease needs to invade host cells to complete its life cycle. Macromolecules on host cell surfaces such as laminin, thrombospondin, heparan sulfate, and fibronectin are believed to be important in mediating parasite-host cell adhesions and in the invasion process of the host cell by the parasite. The SELEX technique (systematic evolution of ligands by exponential enrichment) was used to evolve nuclease-resistant RNA ligands (aptamer = to fit) that bind with affinities of 40-400 nm to parasite receptors for the host cell matrix molecules laminin, fibronectin, thrombospondin, and heparan sulfate. After eight consecutive rounds of in vitro selection four classes of RNA aptamers based on structural similarities were isolated and sequenced. All members of each class shared a common sequence motif and competed with the respective host cell matrix molecule that was used for displacement during the selection procedure. RNA pools following seven and eight selection rounds as well as individual aptamers sharing consensus motifs were active in inhibiting invasion of LLC-MK(2) monkey kidney cells by T. cruzi in vitro.
Ulrich
Henning
H
Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, Caixa Postal 26077, São Paulo 05513-970, Brazil.
Magdesian
Margaret H
MH
Alves
Maria Julia M
MJ
Colli
Walter
W
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D017931:DNA Primers; D006801:Humans; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism / Q000502:physiology*
D017931:DNA Primers; D012313:RNA