Antigen-antibody interactions in capillary electrophoresis.
Immunoreactions in combination with separations by capillary electrophoresis (CE) are increasingly being used to quantitate specific analytes in biological fluids. Both competitive and non-competitive approaches have been used for the purpose and, in selected cases, now compare favorably with conventional quantitative immunoassays with respect to concentration limits of detection. CE is also a useful method to evaluate antigen-antibody binding on-line and offers unique possibilities for binding constant estimates, also for weakly binding antibodies and antibody fragments. In this review we cover recent developments in the use of antigen-antibody interactions in conjunction with CE and conclude that continued development of miniaturization, on-line preconcentration and more sensitive detection schemes will contribute to the further dissemination of CE-based immunoassays building on already established affinity CE approaches.
Heegaard
Niels H H
NH
Department of Autoimmunology, Statens Serum Institute, Copenhagen, Denmark. nhe@ssi.dk
Kennedy
Robert T
RT
D000937:Antigen-Antibody Reactions; D019075:Electrophoresis, Capillary / Q000379:methods*; D007118:Immunoassay / Q000379:methods