Enzyme sensor array for the determination of biogenic amines in food samples.
An enzyme sensor array for the simultaneous determination of the three biogenic amines (histamine, tyramine and putrescine) by pattern recognition using an artificial neural network and its application to different food samples is described. A combination of a monoamine oxidase, a tyramine oxidase and a diamine oxidase (with specific activities sufficient for rapid detection) are immobilised each on a separate screen-printed thick-film electrode via transglutaminase and glutaraldehyde to compare these cross-linking reagents with regard to their suitability. To calculate the amount of a specific biogenic amine, the raw data from multichannel software were transferred to a neural network. The sensor array takes 20 min to complete (excluding statistical data analysis) with only one extraction and subsequent neutralisation step required prior to sensor measurement. The lower detection limits with the enzyme sensor were 10 mg/kg for histamine and tyramine, and 5 mg/kg for putrescine with a linear range up to 200 mg/kg for histamine and tyramine and 100 mg/kg for putrescine. The application area of the enzyme sensor array was tested from fish to meat products, sauerkraut, beer, dairy products, wine and further fermented foods and compared with the data of conventional LC analyses (mean correlation coefficient: 0.854).
Lange
Jana
J
University of Greifswald, Institute for Chemistry and Biochemistry, Germany.
Wittmann
Christine
C
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D001679:Biogenic Amines / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005504:Food Analysis; D006632:Histamine / Q000032:analysis; D016571:Neural Networks, Computer; D011700:Putrescine / Q000032:analysis; D012015:Reference Standards; D014439:Tyramine / Q000032:analysis
D001679:Biogenic Amines; D004800:Enzymes, Immobilized; D006632:Histamine; D000594:Amino Acid Oxidoreductases; D011700:Putrescine; D014439:Tyramine