The expression of vimentin in HL-60 cells induced with etoposide using immunofluorescence and immunogold methods.
HL-60 leukemic cells were treated with 6 different doses of etoposide for 72 hours. Changes in the distribution of vimentin were found to be dependent on the concentration of etoposide. As compared with control cells there were distinct changes in cells incubated with 100 and 200 microM/L of the drug. The size of cells treated with 100 microM/L and especially with 200 microM/L increased, but the number of cells decreased. In control cells and those treated with 0.02, 0.2 and 2 microM/L etoposide, vimentin was seen rather as a ring often with the increased concentration near one pole of the cells. Cells at 20 microM/L etoposide showed the same staining pattern but more brighter cells were found. The addition of 100 microM/L and 200 microM/L etoposide to cells resulted in diffusely distributed fluorescence staining, which often appeared as a quite dense network around the nucleus. Immunogold labelling was observed in cells treated with all doses of etoposide and control cells. Labelling was localized in the nucleus but also in the cytoplasm but rather in the area of the nucleus.
Grzanka
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Department of Clinical Pathomorphology, University School of Medical Sciences, Bydgoszcz Institute of Biology and Environment Protection, Bydgoszcz University of Kazimierz Wielki. grzanka@ab-byd.edu.pl
D000970:Antineoplastic Agents / Q000494:pharmacology*; D048429:Cell Size / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D018922:HL-60 Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D016253:Microscopy, Immunoelectron; D014746:Vimentin / Q000096:biosynthesis* / Q000648:ultrastructure
D000970:Antineoplastic Agents; D014746:Vimentin; D005047:Etoposide