Effects of streptococcal preparation OK-432 on liver regeneration and energy status after partial hepatectomy under ischemia/reperfusion in rats.
OBJECTIVE
Activation of reticuloendothelial system functions by the treatment with OK-432 has been reported to enhance liver regeneration. However, its effect on liver regeneration has not been studied after hepatectomy under ischemia/reperfusion which is in clinical use. The aim was to examine the effect of OK-432 on regeneration and energy status of the liver after hepatectomy under ischemia/reperfusion in rats.


METHODS
Rats were randomly divided into two groups; OK-432 pretreatment and saline treatment (control) group. In the OK-432 group, OK-432 (2.5 mg/kg body weight) was administered intraperitoneally 24 hours before hepatectomy. In the control group, the same volume of physiological saline was administered in the same manner. Seventy percent hepatectomy was performed in both groups during the second 15-minute ischemia period after an initial 15-minute ischemia and 15-minute reperfusion periods. The survival after hepatectomy, relative liver weight, deoxyribonucleic acid synthesis rate, and hepatic adenine nucleotide and energy charge levels were examined immediately after hepatectomy and on postoperative days 1, 2, 3, and 7. Serum levels of total bilirubin, glutamic pyruvic transaminase, and hyaluronic acid were also measured.


RESULTS
All rats survived and the relative liver weight and deoxyribonucleic acid synthesis rate were not significantly different in the two groups. Serum total bilirubin and glutamic pyruvic transaminase levels were not significantly different in both groups. The serum concentration of hyaluronic acid immediately after hepatectomy was significantly higher in the OK-432 group than in the control group. The pretreatment with OK-432 had no significant effect on the levels of adenine nucleotides and energy charge in the liver.


CONCLUSIONS
Under ischemia/reperfusion, pretreatment with OK-432 has no significant effect on regeneration and energy status of the liver after hepatectomy.
Watanabe
Masato
M
Department of Surgery and Oncology, Kyushu University Graduate School of Medicine, Fukuoka 812-8582, Japan.
Chijiiwa
Kazuo
K
Kameoka
Nobuhisa
N
Nakano
Kenji
K
Noshiro
Hirokazu
H
Tanaka
Masao
M
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D004247:DNA / Q000096:biosynthesis; D006498:Hepatectomy; D006820:Hyaluronic Acid / Q000097:blood; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000503:physiopathology; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D023421:Models, Animal; D009929:Organ Size; D010844:Picibanil / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
D000227:Adenine Nucleotides; D010844:Picibanil; D006820:Hyaluronic Acid; D004247:DNA