Comparison of Albicans ID2 agar plate with the germ tube for presumptive identification of Candida albicans.
Albicans ID2 (bioMérieux, France) is a commercially available chromogenic medium that allows rapid and specific macroscopic identification of Candida albicans and facilitates the differentiation of species in mixed cultures. We compared it with the standard method for the identification of yeast species, the germ tube test (GT). This study involved 423 clinical isolates, including 163 C. albicans and 260 non-albicans yeasts. Sensitivity of Albicans ID2 agar plates regarding the identification of C. albicans were 98.2% after 48 h of incubation and specificity of 96.6%. This method using rapid enzymatic method shows the same similar sensitivity than the GT test The false negative rate (1.8%) for the GT test is consistent with that previously reported. None tests discriminated between C. albicans and C. dubliniensis isolates.
Cárdenes
C D
CD
Preventive Medicine and Public Health Department. University of La Laguna, Tenerife, Canary Islands, Spain.
Carrillo
A J
AJ
Arias
A
A
Rodríguez-Alvarez
C
C
Torres-Lana
A
A
Sierra
A
A
Arévalo
M P
MP
D000362:Agar; D002176:Candida albicans / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D002177:Candidiasis / Q000175:diagnosis* / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media; D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
D003470:Culture Media; D000362:Agar