The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus.
To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes.
Endo
Makoto
M
Laboratory of Plant Breeding, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
Matsubara
Hitoshi
H
Kokubun
Takao
T
Masuko
Hiromi
H
Takahata
Yoshihito
Y
Tsuchiya
Tohru
T
Fukuda
Hiroo
H
Demura
Taku
T
Watanabe
Masao
M
D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology*; D000070116:Lotus / Q000235:genetics* / Q000254:growth & development; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018514:Plant Structures / Q000502:physiology*; D015203:Reproducibility of Results; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary