Synthesis of two neuromedin U (NMU) analogues and their comparative effect of reducing food intake in rats.
To examine the roles of aromatic rings, Tyr residues at positions 1 and 5 and Phe residues at positions 16, 17, and 19 of rat neuromedin U-23 (NMU-23) (Tyr-Lys-Val-Asn-Glu-Tyr-Gln-Gly-Pro-Val-Ala-Pro-Ser-Gly-Gly-Phe-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2) for reducing food intake activity in male Wistar rats, two NMU-23 analogues, [Phe(4F)16,17,19] NMU-23 and [Tyr(Me)1,6]NMU-23, were synthesized by Fmoc strategy of manual solid-phase method. The synthetic NMU-23 showed reducing effect on food intake in rats. [Phe(4F)16,17,19]NMU-23 exhibited higher reducing food in take effect than that of NMU-23. On the contrary, [Tyr(- Me)1,6]NMU-23 showed no reducing effect on food intake in rats than that of NMU-23.
Abiko
T
T
Research Laboratory, Global Shinwa Pharmaceutical Co. Ltd., Iwate-gun, Iwate-ken, Japan. lab@mx2.alpha-web.ne.jp
Takamura
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005247:Feeding Behavior / Q000187:drug effects*; D007276:Injections, Intraventricular; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D010649:Phenylalanine / Q000378:metabolism; D015202:Protein Engineering; D051381:Rats; D017208:Rats, Wistar; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000378:metabolism
D009479:Neuropeptides; C056900:neuromedin U; D014443:Tyrosine; D010649:Phenylalanine