title: [Cloning of the human cytochrome p450 2B6 gene in Escherichia coli cells].
abstract: Human cytochrome P450 2B6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+)/CYP 2B6 was expressed in E. coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium.
authors.affiliation: Orekhovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow 119992.
authors.forename: S N
authors.initials: SN
authors.lastname: Kolomeĭchuk
authors.forename: M V
authors.initials: MV
authors.lastname: Pokrovskaia
authors.forename: A A
authors.initials: AA
authors.lastname: Borisova
authors.forename: A A
authors.initials: AA
authors.lastname: Zhgun
authors.forename: L I
authors.initials: LI
authors.lastname: Solodar'
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: V I
authors.initials: VI
authors.lastname: Shvets
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Gervaziev
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
authors.forename: A I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases; D003001:Cloning, Molecular; D065702:Cytochrome P-450 CYP2B6; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D006801:Humans; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating
vernacular_title: Klonirovanie gena tsitokhroma p450 2B6 cheloveka v kletkakh Escherichia coli.