title: Microscope-based techniques to study cell adhesion and migration.
abstract: Modern light microscopy has evolved to provide a variety of quantitative imaging techniques and also the capability to perturb structure-function relationships in living cells. The advances have been especially useful in the study of cell adhesion and migration. This review will focus on how such microscopy-based techniques can be useful in situ to study the molecular interactions and dynamics, to locally perturb actin-based structures and to measure the traction forces exerted by motile cells.
authors.affiliation: Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7090 USA.
authors.forename: Partha
authors.initials: P
authors.lastname: Roy
authors.forename: Zenon
authors.initials: Z
authors.lastname: Rajfur
authors.forename: Pawel
authors.initials: P
authors.lastname: Pomorski
authors.forename: Ken
authors.initials: K
authors.lastname: Jacobson
mesh_terms: D000199:Actins / Q000648:ultrastructure; D001692:Biological Transport; D002448:Cell Adhesion; D002465:Cell Movement; D003584:Cytological Techniques; D004735:Energy Transfer; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000199:Actins