[Simultaneous determination of aspartame and amino acids in fermented milk beverages by HPLC].
An RP-HPLC method for the simultaneous determination of aspartame and amino acids in fermented milk beverages were established. Samples were prepared by mixing with methanol at a ratio of 1:1 and centrifuged at 4,000 r/min for 15 min. Ten microL supernatant was moved into a sample tube, dried and derivatized according to PICO-TAG procedure developed by Waters. A Novapak C18 column (3.9 mm x 150 mm) was used instead of PICO TAG column and the gradient elution program was modified correspondingly. Column temperature was maintained at 38 degrees C and the components were detected at 254 nm. Linearity for aspartame at the range in 1-100 mg/L is A = 333 C + 20 with r = 0.9996 where A is the integrated area of chromatographic peak of aspartame and C is the corresponding mass concentration. Repeatability for 8 injections was tested and the RSD of 3.2% was obtained. The recovery of aspartame for 5 samples with the method ranged from 94.2% to 98.7%.
Zhang
W
W
Zhejiang Institute of Microbiology, Hangzhou, 310012.
Sun
X
X
She
Q
Q
Zhang
X
X
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D001218:Aspartame / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005285:Fermentation; D008892:Milk / Q000737:chemistry*
D000596:Amino Acids; D001218:Aspartame