title: Ruminal metabolism in sheep of saponins from Yucca schidigera.
abstract: Fifty-five mg per kg live weight of crystallized Yucca schidigera saponins, corresponding to 26 mg/kg live weight of sapogenins, was given daily intraruminally to two lambs for 11 consecutive days. Neither of the lambs showed any sign of toxicity throughout the experimental period. One lamb was killed 5 h after the last dose and GC-MS analysis of the free and conjugated sapogenin content samples of liver, and of the contents of the rumen, omasum, abomasum, duodenum, jejunum, ileum, colon and rectum, of faecal samples collected before dosing started, and of parts of the administered Yucca saponin were performed. The Yucca material contained mainly sarsasapogenin and smilagenin saponins. Ingested saponins were quickly hydrolysed in the rumen to free sapogenins and, in part, epimerized at C-3 to afford episapogenins. The absorption of free sapogenins appeared to occur in the jejunum. The metabolism of Yucca saponins was identical to that of Narthecium ossifragum saponins, and it is suggested that Yucca saponins could replace N. ossifragum saponins for toxicity studies on the latter plant.
authors.affiliation: National Veterinary Institute, Oslo, Norway.
authors.forename: A
authors.initials: A
authors.lastname: Flåøyen
authors.forename: A L
authors.initials: AL
authors.lastname: Wilkins
authors.forename: M
authors.initials: M
authors.lastname: Sandvik
mesh_terms: D000818:Animals; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007408:Intestinal Absorption; D008297:Male; D015394:Molecular Structure; D010787:Photosensitivity Disorders; D010947:Plants, Toxic; D012503:Saponins / Q000008:administration & dosage* / Q000378:metabolism* / Q000493:pharmacokinetics / Q000633:toxicity; D012756:Sheep / Q000378:metabolism*; D018675:Toxicity Tests / Q000379:methods*; D027623:Yucca
chemical_list: D012503:Saponins