[Study of population dissociation of collection strains of Erwinia carotovora].
Unusual dissociants of Erwinia carotovora subsp. atroseptica (EAT) population that emerge with high frequency (18-94%) during long-term storage of cultures were found. The dissociants (Poe-phenotype) differ from parental forms by slow growth and long development of the colour of colonies on EMB agar though they are not Lac-mutants. More than 60% of Poe-mutants of strain EAT 39A are characterized by 8- and 150-fold decrease of stability to erythromycin and oleandomicin, correspondingly. They are also sensitive to colicin-like carotovoricin, which is induced by mytomicin C, to carotovoricin of initial strain EAT 39A and to autocin, which they produce themselves during induction. A multiple phenotype of these dissociants (dissociants of the second type) is labeled: PoeOmSErSBnSAu. Its appearance as well as the appearance of pure Poe-variants is not associated with usual mutations and a loss of residential plasmids by cells. Population dissociants of the both types--Poe and PoeApSOmS--were also obtained from E. carotovora subsp. carotovora (J2) during growth at supraoptimal temperature. Poe-variants are more often observed in representatives of subspecies atroseptica than carotovora and can be useful when studying pathogeneity of these practically important erwinias.
Tovkach
F I
FI
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Tovkach
A F
AF
Moroz
S N
SN
D016973:Pectobacterium carotovorum / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010641:Phenotype; D013696:Temperature; D013997:Time Factors
Izuchenie populiatsionnoĭ dissotsiatsii kollektsionnykh shtammov Erwinia carotovora.