TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.
A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5'-ACGGA-3' and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found in Thermus sp. GW cells.
Zylicz-Stachula
Agnieszka
A
EURx Ltd, ul. Jaíkowa Dolina 29, 80-286 Gdaásk, Poland.
Harasimowicz-Slowińska
Renata I
RI
Sobolewski
Ireneusz
I
Skowron
Piotr M
PM
D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D006358:Hot Temperature; D013824:Thermus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific