Vibration injury damages arterial endothelial cells.
Prolonged exposure to hand-transmitted vibration can cause debilitating neural and vascular dysfunction in humans. It is unclear whether the pathophysiology involves simultaneous or sequential injury of arteries and nerves. The mechanism of vibration injury was investigated in a rat tail model, containing arteries and nerves structurally similar to those in the human hand. Tails were selectively vibrated for 1 or 9 days with the remainder of the animal at rest. One vibration bout of 4 h/day, 60 HZ, 5 g (49 m/s(2)) acceleration, injured endothelial cells. Injury was signaled by elevated immunostaining for NFATc3 transcription factor. Electron microscopy revealed that vibration for 9 days produced loss and thinning of endothelial cells, with activated platelets coating the exposed subendothelial tissue. Endothelial cells and arterial smooth muscle cells contained double membrane-limited, swollen processes indicative of vasoconstriction-induced damage. Laser doppler surface recording demonstrated that 5 min of vibration significantly diminished tissue blood perfusion. These findings indicate that early injury involves vasoconstriction and denuding of the arterial endothelium.
Curry
Brian D
BD
Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA.
Bain
James L W
JL
Yan
Ji-Geng
JG
Zhang
Lin Ling
LL
Yamaguchi
Mark
M
Matloub
Hani S
HS
Riley
Danny A
DA
D000818:Animals; D001158:Arteries / Q000293:injuries* / Q000473:pathology* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000293:injuries* / Q000473:pathology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D050778:NFATC Transcription Factors; D009784:Occupational Diseases / Q000209:etiology* / Q000473:pathology* / Q000503:physiopathology; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D012149:Restraint, Physical / Q000523:psychology; D013623:Tail / Q000098:blood supply / Q000293:injuries / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism; D014732:Vibration / Q000009:adverse effects*
D004268:DNA-Binding Proteins; D050778:NFATC Transcription Factors; D014157:Transcription Factors; C445503:transcription factor NF-AT c3