Genomic organization of the human gene HEP27: alternative promoter usage in HepG2 cells and monocyte-derived dendritic cells.
We used representational difference analysis to discover new genes with specific expression in dendritic cells. Among other genes, we identified HEP27, encoding a member of the short chain alcohol dehydrogenase/reductase family to be upregulated during monocyte to dendritic cell differentiation. Originally cloned from hepatocellular carcinoma cells (HepG2), HEP27 was exclusively expressed in monocyte-derived dendritic cells within the hematopoietic system. The presence of different transcripts in monocyte-derived dendritic cells, HepG2 cells, and various tissues could be traced back to alternative splicing and alternative promoter usage. We describe here the complete genomic organization of HEP27, including two alternative promoter regions: a hepatocyte-specific promoter which was induced by the histone deacetylase inhibitor sodium butyrate in several other cell types, and a second upstream promoter which was specifically active in monocyte-derived dendritic cells. Its exclusive usage in monocyte-derived dendritic cells makes the alternative HEP27 promoter an interesting target to study dendritic-cell-specific gene regulation.
Heinz
Sven
S
Department of Hematology and Oncology, University Hospital, 93042 Regensburg, Germany.
Krause
Stefan W
SW
Gabrielli
Franco
F
Wagner
Harald M
HM
Andreesen
Reinhard
R
Rehli
Michael
M
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000429:Alcohol Oxidoreductases; D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D002087:Butyrates / Q000494:pharmacology; D000074409:Carbonyl Reductase (NADPH); D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D002087:Butyrates; D009687:Nuclear Proteins; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; D000074409:Carbonyl Reductase (NADPH); C095961:DHRS2 protein, human