Spectrophotometric, spectrofluorimetric, HPLC and CZE determination of mirtazapine in pharmaceutical tablets.
Four analytical methods have been developed for the quality control of tablets containing mirtazapine: spectrophotometry, spectrofluorimetry, high performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE). All the methods only require a simple extraction procedure of mirtazapine from the tablets before analysis. The concentration of mirtazapine in solutions was determined in the linearity range of 5-25 microg/ml at lambda=315 nm for spectrophotometry and at lambda=220 nm for HPLC and CZE. Spectrofluorimetric determinations were achieved at lambda(excitation)=328 nm and lambda(emission)=415 nm in the linearity range of 2-25 ng/ml. All the methods gave similar results and were validated for selectivity, linearity, precision and sensitivity. Spectrometric methods gave slightly higher RSD values (up to 2.54%). The four methods were directly and easily applied to the pharmaceutical preparation with accuracy, resulting from recovery experiments between 99.72% in HPLC and 101.47% in spectrofluorimetry.
Labat
L
L
Laboratoire de Chimie Analytique, Faculté de Pharmacie, 3 Place de la Victoire, F-33076 Cedex, Bordeaux, France. laurence.labat@u-bordeaux2.fr
Dallet
P
P
Kummer
E
E
Dubost
J P
JP
D000928:Antidepressive Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D008803:Mianserin / Q000031:analogs & derivatives* / Q000032:analysis*; D000078785:Mirtazapine; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets
D000928:Antidepressive Agents; D007202:Indicators and Reagents; D012996:Solutions; D013607:Tablets; D008803:Mianserin; D000078785:Mirtazapine