title: Clonal expansion T cells identified in acute monoblastic leukemia by CDR3 size analysis of TCR V beta repertoire using RT-PCR and genescan.
abstract: OBJECTIVE
  To investigate the distribution and clonality of T cell receptor (TCR) V beta repertoire in patients with acute monoblastic leukemia (AML-M5).
  
  
  METHODS
  Expression of the TCR V beta repertoire was analyzed using reverse transcription-polymerase chain reaction (RT-PCR), which amplified the complementarity determining region 3 of 24 TCR V beta genes in peripheral blood from 9 cases with acute myclogenous leukemia subtype 5 or acute monoblastic leukemia (AML-M5). PCR products were further studied by genescan analysis to identify T cell clonality.
  
  
  RESULTS
  Expression of 1-10 V beta subfamilies was found in samples from 9 patients. Genescan analysis showed that some V beta subfamily products from 8 of 9 cases contained an oligoclonal peak. Oligoclonal T cells of the V beta 2 subfamily could be found in 6 patients with AML-M5.
  
  
  CONCLUSIONS
  T cell clonality expansion was found in AML-M5 cases and were tendentious in the V beta 2 subfamily, suggesting a the specific immune response for leukemia cell (M5) associated antigen and may display antileukemia activity.
authors.affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
authors.forename: Yangqiu
authors.initials: Y
authors.lastname: Li
authors.forename: Lijian
authors.initials: L
authors.lastname: Yang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Chen
authors.forename: Rongfu
authors.initials: R
authors.lastname: Li
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lu
authors.forename: Gengxin
authors.initials: G
authors.lastname: Luo
mesh_terms: D022801:Complementarity Determining Regions / Q000235:genetics*; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D022801:Complementarity Determining Regions