N-acetyl-D-glucosamine specific hemagglutinin receptor of Vibrio cholerae O1 in chicken erythrocyte membranes.
N-Acetyl-D-glucosamine specific cell-associated hemagglutinin (HA)/lectin, previously purified from a strain of Vibrio cholerae O1, had been established as an adhesin molecule of V. cholerae O1 cells. This communication records the isolation and purification of the glycoprotein receptor of the N-acetyl-D-glucosamine specific HA of the V. cholerae O1 strain from chicken erythrocyte membranes. The most salient feature of this study is that the pretreatment of partially purified glycoprotein with purified HA could completely inhibit the hemagglutinating activity of the V. cholerae O1 strain with chicken erythrocytes.
Sasmal
D
D
Department of Biochemistry, National Institute of Cholera and Enteric Diseases, P-33, C I T Road, Scheme XM, Beliaghata, P.O. Box 177, 700010, Kolkata, India. icmrnicd@ren.nic.in
Guhathakurta
B
B
Bhattacharya
S K
SK
Pal
C R
CR
Datta
A
A
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000378:metabolism*; D014734:Vibrio cholerae / Q000378:metabolism*
D006388:Hemagglutinins; D000117:Acetylglucosamine