title: Efficacy of dry-cleaning in removing Fel d 1 allergen from wool fabric exposed to cats.
abstract: BACKGROUND
  The main cat allergen (Fel d 1) is ubiquitous, having been found even in indoor environments and public places where a cat has never been kept. Clothes of cat owners constitute a carrier for the distribution of Fel d 1 allergen in these environments. Schools, for example, may be a site of indirect exposure to cat allergens.
  
  
  OBJECTIVE
  Our goal was to investigate the efficacy of commercial dry-cleaning in removing cat allergens from wool fabrics that had been exposed to cats to evaluate a possible preventive procedure.
  
  
  METHODS
  Twenty-six identical wool "squares" (80 x 100 cm) were put in cat baskets for 1 week. In our laboratory, the squares were cut in half (40 x 50 cm), and one half was subjected to high-volume sampling for 5 minutes in a cat-free room. The other half was subjected to commercial dry-cleaning and then the high-volume sampling. Five wool squares not exposed to cats served as controls. Dust was collected from the wool squares with a high-volume air sampler. Particulate material was harvested onto glass fiber filters (AP 20 Millipore, Milan, Italy) with 25-mm diameter and 2-microm pore size. Each dust sample was assayed by affinity-purified monoclonal antibody against purified Fel d 1. The results were expressed as micrograms per filter. Statistical analysis was done by using the paired t test.
  
  
  RESULTS
  Before dry-cleaning, Fel d 1 allergen was detected on all cat-exposed wool squares. No appreciable cat allergen was detected on control materials. After commercial dry-cleaning, the amounts of Fel d 1 extracted from cat-exposed squares were significantly reduced (t = 14.63; P < 0.001) but not abolished. Three of the five control squares were contaminated by Fel d 1.
  
  
  CONCLUSIONS
  Commercial dry-cleaning effectively removes large amounts of cat allergen from wool materials exposed to cats but does not completely abolish this protein. Further, low Fel d 1 contamination may occur during this procedure.
authors.affiliation: Department of Chest Diseases, Hospital A. Cardarelli, Naples, Italy. gennaro.liccardi@tin.it
authors.forename: Gennaro
authors.initials: G
authors.lastname: Liccardi
authors.forename: Maria
authors.initials: M
authors.lastname: Russo
authors.forename: Domingo
authors.initials: D
authors.lastname: Barber
authors.forename: Jean Anne
authors.initials: JA
authors.lastname: Gilder
authors.forename: Perna Felice
authors.initials: PF
authors.lastname: Di
authors.forename: Silvano
authors.initials: S
authors.lastname: Parmiani
authors.forename: Maria
authors.initials: M
authors.lastname: D'Amato
authors.forename: Gennaro
authors.initials: G
authors.lastname: D'Amato
mesh_terms: D000818:Animals; D002415:Cats; D006023:Glycoproteins / Q000302:isolation & purification*; D014935:Wool
chemical_list: D006023:Glycoproteins; C059296:Fel d 1 protein, Felis domesticus