Interleukin-8 induction and adhesion of the coccoid form of Helicobacter pylori.
To determine the pathological significance of the coccoid form of Helicobacter pylori, its adhesion to and induction of secretion of interleukin-8 (IL-8) by gastric epithelial (MKN45) cells were studied. By flow cytometry, the adhesion of the coccoid form to MKN45 cells was significantly lower than that of the helical form. The monoclonal antibody A20 recognising lipopolysaccharide of H. pylori inhibited the adhesion of the coccoid form to MKN45 cells as much as that of the helical form. There was significantly lower induction of IL-8 secretion by MKN45 cells exposed to the coccoid form (two of four strains) as compared with the helical form.
Osaki
Takako
T
Yamaguchi
Hiroyuki
H
Taguchi
Haruhiko
H
Fukada
Minoru
M
Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
Kawakami
Hayato
H
Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
Hirano
Hiroshi
H
Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
Kamiya
Shigeru
S
D000693:Anaerobiosis; D001422:Bacterial Adhesion / Q000276:immunology; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000276:immunology* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured
D016209:Interleukin-8