title: Discrepancies in the immunophenotype of lymphoma cells in samples obtained simultaneously from different anatomic sites.
abstract: Few studies have compared the immunophenotypic profiles of non-Hodgkin lymphoma (NHL) cells obtained simultaneously from different anatomic sites. In the present study, we compared flow cytometry immunophenotypic results in 64 consecutive NHL cases in which aspiration or biopsy of 2 sites was performed within 30 days to assess the potential discrepancy rate. In 14 cases (22%), discordant antigen expression was identified, including 4 (36%) of 11 cases with discordant morphologic features and 10 (19%) of 53 cases with concordant morphologic features in the 2 samples. Discrepancies involved 1 antigen in 10 patients and 2 antigens in 4 patients. Antigens most frequently discrepant included CD5 (n = 4), FMC7 (n = 3), and CD20 (n = 3). We conclude that the immunophenotype of NHL cells is generally stable, yet discrepancies can occur in a subset of patients. Differences in immunophenotype may relate to mechanisms of disease dissemination, influence of the microenvironment, or differential response to therapy.
authors.affiliation: Department of Hematopathology, University of Texas, M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Onciu
authors.forename: Su G
authors.initials: SG
authors.lastname: Berrak
authors.forename: L Jeffrey
authors.initials: LJ
authors.lastname: Medeiros
authors.forename: Ruth L
authors.initials: RL
authors.lastname: Katz
authors.forename: Yang O
authors.initials: YO
authors.lastname: Huh
mesh_terms: D000328:Adult; D000368:Aged; D018951:Antigens, CD20 / Q000032:analysis; D001706:Biopsy; D001707:Biopsy, Needle; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D018956:CD5 Antigens / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000032:analysis; D012189:Retrospective Studies
chemical_list: D018951:Antigens, CD20; D018956:CD5 Antigens; D006023:Glycoproteins; C112523:MS4A1 protein, human; D017455:Receptors, IgE