Ultrastructural alterations induced by nifurtimox and another nitro derivative on epimastigotes of Trypanosoma cruzi.
We report the ultrastructural alterations induced on epimastigotes by nifurtimox and 5-nitro-2-thienyl-malononitrile (5NO2TM), a novel compound with anti-Trypanosoma cruzi activity. Parasites treated with concentrations of nifurtimox lower than usually employed for this kind of study showed vacuolisation, alterations of the mitochondria, the nucleus and the ribosomes. 5NO2TM caused the same kind of damage, but to a greater degree. This result correlates with the fact that cultures treated with this compound experienced a greater loss of viability.
Muelas-Serrano
S
S
Departamento de Parasitologia, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, Madrid, Spain
Pérez-Serrano
J
J
Gómez-Barrio
A
A
Arán
V J
VJ
Rodríguez-Caabeiro
F
F
D000818:Animals; D002478:Cells, Cultured; D008018:Life Cycle Stages; D008314:Malonates / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D008928:Mitochondria / Q000187:drug effects; D009547:Nifurtimox / Q000494:pharmacology* / Q000633:toxicity; D009570:Nitriles / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D009574:Nitro Compounds / Q000494:pharmacology / Q000633:toxicity; D021261:Parasitic Sensitivity Tests; D013997:Time Factors; D014344:Trypanocidal Agents / Q000494:pharmacology* / Q000633:toxicity; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*
C466759:5-nitro-2-thienylmalononitrile; D008314:Malonates; D009570:Nitriles; D009574:Nitro Compounds; D014344:Trypanocidal Agents; D009547:Nifurtimox