Specificity of a heme-assimilating system of Vibrio vulnificus to synthetic heme compounds.
Vibrio vulnificus strain L-180, a clinical isolate, can obtain iron from a synthetic heme, iron-tetra(4-sulfonatophenyl)porphyrin (Fe-TPPS), as well as from a natural heme, protoheme. This assimilation of iron bound to TPPS was demonstrated to be a common property of V. vulnificus by testing a total of 27 strains isolated from both clinical and environmental sources. Strain L-180 could also utilize Fe-TCPP, but not Fe-TMPyP, as a sole iron source. TPPS or its complex with a metal ion reduced bacterial multiplication in the broth containing a minimum dose of Fe-TPPS. When inoculated into human serum supplemented with Fe-TCPP, L-180 could grow only in the presence of a protease from the same bacterium. In both TPPS and TCPP, each side chain of a porphyrin ring has a negative charge. Therefore, this negative charge may be important for interaction with an outer membrane receptor involving in a heme-assimilating system of V. vulnificus.
Miyoshi
Shin-ichi
S
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, 700-8530, Okayama, Japan. miyoshi@pharm.okayama-u.ac.jp
Kamei
Takehito
T
Ota
Yoko
Y
Masunaga
Chiaki
C
Izuhara
Yuko
Y
Tomochika
Ken-ichi
K
Shinoda
Sumio
S
Yamamoto
Shigeo
S
D000818:Animals; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004783:Environmental Microbiology; D006418:Heme / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008665:Metalloporphyrins / Q000378:metabolism*; D051379:Mice; D013379:Substrate Specificity; D014733:Vibrio / Q000254:growth & development / Q000378:metabolism*; D014735:Vibrio Infections / Q000382:microbiology
D003470:Culture Media; D008665:Metalloporphyrins; D006418:Heme; D007501:Iron