Optimization of method for detecting and characterizing NOM by HPLC-size exclusion chromatography with UV and on-line DOC detection.
High-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with ultraviolet absorbance (UVA) and on-line dissolved organic carbon (DOC) detectors has been adapted and optimized under various conditions. An enhanced HPSEC-UVA system employing a modified commercially available DOC detector provides a better understanding of the qualitative and quantitative natural organic matter (NOM) properties in water samples by detecting aromatic and nonaromatic fractions of NOM as a function of molecular weight (MW). The most critical merit of this system is that the DOC detector is readily available and widely used. With only a few modifications, a commercially available TOC analyzer served as a DOC detector, integrated with the HPSEC to measure DOC along with UVA, and provided a specific UVA (SUVA) chromatograph that is useful information for drinking water plant design and operation. Without preconcentration, samples can be analyzed with a small amount of sample, with a DOC detection limit as low as 0.1 mg/L (as DOC).
Her
Namguk
N
Environmental Engineering, University of Colorado, Boulder 80309, USA. her@ucsub.colorado.edu
Amy
Gary
G
Foss
David
D
Cho
Jaeweon
J
Yoon
Yeomin
Y
Kosenka
Paul
P
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D008970:Molecular Weight; D009930:Organic Chemicals / Q000032:analysis*; D010316:Particle Size; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays; D014873:Water Pollutants / Q000032:analysis*
D009930:Organic Chemicals; D014873:Water Pollutants