Detection of tissues of the central nervous system (CNS) as specified risk material (SRM) in meat products by means of gas chromatography-mass spectrometry (GC-MS).
Determination of specified risk material (SRM) in processed meat products was performed by quantification of brain specific fatty acids using gas chromatography-mass spectrometry (GC-MS). Results from SMP (internal standardised meat products) based analyses showed that absolute concentrations of CNS are correlated (r2 = > 0.97) with the contents of the CNS typical fatty acids docosahexaenoic acid (C 22:6), nervonic acid (C 24:1), lignoceric acid (C 24) and cerebronic acid (C 24oh). GC-MS detection limits were measured at 0.01% CNS. The cut off value was calculated at 0.39% (w/w) CNS in SMP. In a controlled blindfold experiment we were able to identify correctly all positive and negative SMP samples, respectively. Our results indicate that GC-MS based SRM detection may serve as a reference method for immunochemical and immunohistochemical determination of SRM in processed meat products.
Biedermann
Wolfgang
W
Institut für Tierhygiene und Offentliches Veterinärwesen, Universität Leipzig, D-04103 Leipzig. wbieder@vmf.uni-leipzig.de
Lücker
Ernst
E
Hensel
Andreas
A
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002490:Central Nervous System / Q000737:chemistry; D003257:Consumer Product Safety; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis* / Q000517:prevention & control / Q000635:transmission; D005227:Fatty Acids / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008461:Meat Products / Q000032:analysis* / Q000821:virology; D012016:Reference Values
D005227:Fatty Acids