The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.).
The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.
Magnadóttir
Bergljót
B
Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland. bergmagn@hi.is
Crispin
Max
M
Royle
Louise
L
Colominas
Cristina
C
Harvey
David J
DJ
Dwek
Raymond A
RA
Rudd
Pauline M
PM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002853:Chromatography, Liquid / Q000662:veterinary; D010450:Endopeptidases / Q000378:metabolism; D005399:Fishes / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000737:chemistry*; D007075:Immunoglobulin M / Q000097:blood / Q000737:chemistry*; D013058:Mass Spectrometry / Q000662:veterinary; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000276:immunology
D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D009844:Oligosaccharides; C038601:trinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D010450:Endopeptidases