MERP1: a mammalian ependymin-related protein gene differentially expressed in hematopoietic cells.
We have utilized differential display polymerase chain reaction to investigate the gene expression of hematopoietic progenitor cells from adult bone marrow and umbilical cord blood. A differentially expressed gene was identified in CD34+ hematopoietic progenitor cells, with low expression in CD34- cells. We have obtained the full coding sequence of this gene which we designated human mammalian ependymin-related protein 1 (MERP1). Expression of MERP1 was found in a variety of normal human tissues, and is 4- and 10-fold higher in adult bone marrow and umbilical cord blood CD34+ cells, respectively, compared to CD34- cells. Additionally, MERP1 expression in a hematopoietic stem cell enriched population was down-regulated with proliferation and differentiation. Conceptual translation of the MERP1 open reading frame reveals significant homology to two families of glycoprotein calcium-dependant cell adhesion molecules: ependymins and protocadherins.
Gregorio-King
Claudia C
CC
Stem Cell Laboratory, The Douglas Hocking Research Institute, Barwon Health, The Geelong Hospital, Geelong, VIC, 3220, Australia. claudiag@barwonhealth.org.au
McLeod
Janet L
JL
Collier
Fiona McL
FM
Collier
Gregory R
GR
Bolton
Karyn A
KA
Van Der Meer
Gavin J
GJ
Apostolopoulos
Jim
J
Kirkland
Mark A
MA
D000328:Adult; D000595:Amino Acid Sequence; D018952:Antigens, CD34 / Q000032:analysis; D001483:Base Sequence; D015152:Blotting, Northern; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D019295:Computational Biology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D036341:Intercellular Signaling Peptides and Proteins
D018952:Antigens, CD34; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C456603:EPDR1 protein, human; D036341:Intercellular Signaling Peptides and Proteins