Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase.
Triphosphate of a new fluorescent labeled thymidine analogue was incorporated as a substrate for PCR using KOD Dash DNA polymerase forming the corresponding fluorescent labeled DNA which is useful for a DNA probe.
Obayashi
Tsutomu
T
Department of Applied Chemistry, Gunma University, Kiryu, 376-8515, Gunma, Japan.
Masud
Mohammed M
MM
Ozaki
Akiko N
AN
Ozaki
Hiroaki
H
Kuwahara
Masayasu
M
Sawai
Hiroaki
H
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D015342:DNA Probes; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013936:Thymidine / Q000378:metabolism*
D015342:DNA Probes; D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine