title: Mamu-DQA1 allele and genotype frequencies in a randomly sampled breeding colony of rhesus macaques (Macaca mulatta).
abstract: We studied the allelic and genotypic distribution of the major histocompatibility class-II locus DQA1 observed in a random sample of Indian rhesus macaques (Macaca mulatta) from a major breeding facility in the United States. The DNA was isolated from whole blood samples collected between 1991 and 1994 from 65 Indian rhesus monkeys. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), which involves use of specific amplification of DQA1 exon 2 and subsequent restriction digestion of the 242-base pair fragment, was used to genotype the animals for the 20 known macaque (Mamu)-DQA1 alleles. Frequencies for four alleles (DQA1*240x, *2502, *2503 and *0102) differed significantly from those reported in a smaller sample of rhesus macaques from the German Primate Center. The modest genetic survey of Mamu-DQA1 genotypes presented here will be particularly useful in designing epidemiologic studies that investigate associations between immunogenetic background and disease susceptibility in macaque models of human disease.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California at Davis, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Rolfs
authors.forename: J G
authors.initials: JG
authors.lastname: Lorenz
authors.forename: C C
authors.initials: CC
authors.lastname: Wu
authors.forename: N W
authors.initials: NW
authors.lastname: Lerche
authors.forename: D G
authors.initials: DG
authors.lastname: Smith
mesh_terms: D000483:Alleles; D000818:Animals; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D012494:Sampling Studies
chemical_list: D000949:Histocompatibility Antigens Class II; C092258:Mamu-DQA1 protein, Macaca mulatta