title: Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells.
abstract: The calcyclin-binding protein (CacyBP) binds calcyclin (S100A6) at physiological levels of [Ca(2+)] and is highly expressed in brain neurons. Subcellular localization of CacyBP was examined in neurons and neuroblastoma NB-2a cells at different [Ca(2+)](i). Immunostaining indicates that CacyBP is present in the cytoplasm of unstimulated cultured neurons in which resting [Ca(2+)](i) is known to be approximately 50 nm. When [Ca(2+)](i) was increased to above 300 nm by KCl treatment, the immunostaining was mainly apparent as a ring around the nucleus. Such perinuclear localization of CacyBP was observed in untreated neuroblastoma NB-2a cells in which [Ca(2+)](i) is approximately 120 nm. An additional increase in [Ca(2+)](i) to above 300 nm by thapsigargin treatment did not change CacyBP localization. However, when [Ca(2+)](i) in NB-2a cells dropped to 70 nm, because of BAPTA/AM treatment, perinuclear localization was diminished. Ca(2+)-induced translocation of CacyBP was confirmed by immunogold electron microscopy and by fluorescence of NB-2a cells transfected with an EGFP-CacyBP vector. Recombinant CacyBP can be phosphorylated by protein kinase C in vitro. In untreated neuroblastoma NB-2a cells, CacyBP is phosphorylated on a serine residue(s), but exists in the dephosphorylated form in BAPTA/AM-treated cells. Thus, phosphorylation of CacyBP occurs in the same [Ca(2+)](i) range that leads to its perinuclear translocation.
authors.affiliation: Nencki Institute of Experimental Biology, Warsaw, Poland. anfil@nencki.gov.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Filipek
authors.forename: Beata
authors.initials: B
authors.lastname: Jastrzebska
authors.forename: Marcin
authors.initials: M
authors.lastname: Nowotny
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kwiatkowska
authors.forename: Michal
authors.initials: M
authors.lastname: Hetman
authors.forename: Liliana
authors.initials: L
authors.lastname: Surmacz
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Wyroba
authors.forename: Jacek
authors.initials: J
authors.lastname: Kuznicki
mesh_terms: D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
chemical_list: C491777:CacyBP protein, rat; D002135:Calcium-Binding Proteins; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D002118:Calcium