Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop.
The genome of Saccharomyces cerevisiae encodes three close homologues of the Escherichia coli 2'-O-rRNA methyltransferase FtsJ/RrmJ, designated Trm7p, Spb1p and Mrm2p. We present evidence that Trm7p methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop, both in vivo and in vitro. In a trm7Delta strain, which is viable but grows slowly, translation is impaired, thus indicating that these tRNA modifications could be important for translation efficiency. We discuss the emergence of a family of three 2'-O-RNA methyltransferases in Eukaryota and one in Prokaryota from a common ancestor. We propose that each eukaryotic enzyme is located in a different cell compartment, in which it would methylate a different RNA that can adopt a very similar secondary structure.
Pintard
Lionel
L
Centre de Recherche de Biochimie Macromoléculaire du CNRS, 1919 Route de Mende, F-34293 Montpellier cedex 5, France.
Lecointe
François
F
Bujnicki
Janusz M
JM
Bonnerot
Claire
C
Grosjean
Henri
H
Lapeyre
Bruno
B
D000595:Amino Acid Sequence; D000818:Animals; D000926:Anticodon / Q000378:metabolism*; D001483:Base Sequence; D002384:Catalysis; D018797:Cell Cycle Proteins / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology; D005057:Eukaryotic Cells; D006801:Humans; D008780:Methyltransferases / Q000737:chemistry; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger; D012343:RNA, Transfer / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism; D012266:Ribose / Q000031:analogs & derivatives / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012359:tRNA Methyltransferases / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*
D000926:Anticodon; D018797:Cell Cycle Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C015362:D-2-O-methylribose; D012266:Ribose; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D008780:Methyltransferases; C456024:Trm7 protein, S cerevisiae; C102247:rlmE protein, E coli; D012359:tRNA Methyltransferases