[Effect of rhubarb on oxygen radicals leakage from mitochondria of intestinal mucosa in burned rats].
OBJECTIVE
To investigate the effects of rhubarb on oxygen radicals leakage from the mitochondria of intestinal mucosa in burned rats.


METHODS
The activity of cytochrome oxidase and superoxide dismutase (SOD) in mitochondria isolated from intestinal mucosa of burn model of rats was used to evaluate the oxygen radicals leakage from the mitochondria. And the SOD and malondialdehyde (MDA) levels in plasma, liver, intestinal mucosa and its mitochondria were also determined.


RESULTS
After burn injury, the activity of cytochrome oxidase in intestinal mucosa decreased as the time elapses, rhubarb could improve it obviously. Furthermore, rhubarb could reduce the massive consumption of SOD and production of MDA obviously increased in plasma, liver, intestinal mucosa and its mitochondria after burning.


CONCLUSIONS
Rhubarb could enhance the activity of cytochrome oxidase and SOD in small intestine mucosal epithelia in burned rats, thus the oxygen radicals leakage from the mitochondria to be reduced.
Chen
D
D
Changzheng Hospital, Second Military Medical University, Shanghai (200003).
Qiao
L
L
Jing
B
B
D000818:Animals; D002056:Burns / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005609:Free Radicals; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012250:Rheum
D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D010100:Oxygen