The cytochrome P450 complement (CYPome) of Streptomyces coelicolor A3(2).
In the present study we describe the complete cytochrome P450 complement, the "CYPome," of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.
Lamb
David C
DC
Wolfson Laboratory of P450 Biodiversity, Institute of Biological Sciences, University of Wales Aberystwyth, Aberystwyth, Wales SY23 3DA, United Kingdom.
Skaug
Tove
T
Song
Hong-Lin
HL
Jackson
Colin J
CJ
Podust
Larissa M
LM
Waterman
Michael R
MR
Kell
Douglas B
DB
Kelly
Diane E
DE
Kelly
Steven L
SL
D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*
D007527:Isoenzymes; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System