title: Evidence of differences in the biotransformation of organic contaminants in three species of freshwater invertebrates.
abstract: Acute static bioassays were performed using three freshwater invertebrate species (the oligochaete Lumbriculus variegatus, the fingernail clam Sphaerium corneum and the larvae Chironomus riparius) exposed separately to a variety of 14C radiolabelled contaminants. The aim of this work was to investigate if the chemicals remained as parent compounds after the treatments. Chemicals used were 2,4-dichlorophenol; 2,4,5-trichlorophenol; pentachlorophenol; pyrene; Fenpropidin, and Trifluralin. Homogenates of the whole body tissue of each organism were prepared and total radioactivity was measured. Contaminants were then extracted into organic solvents and analysed by high-pressure liquid chromatography techniques. Chromatograms showed that most of the substances extracted were present as parent compounds in S. corneum and in L. variegatus. In contrast, for C. riparius a low proportion of the chemicals was recovered as parent compounds. These results suggest that different metabolic processes could take place in the different species.
authors.affiliation: Department of Biological Chemistry, Faculty of Exact and Natural Sciences, University of Buenos Aires, Argentina. noev@qb.fcen.uba.ar
authors.forename: N R
authors.initials: NR
authors.lastname: Verrengia Guerrero
authors.forename: M G
authors.initials: MG
authors.lastname: Taylor
authors.forename: N A
authors.initials: NA
authors.lastname: Davies
authors.forename: M A M
authors.initials: MA
authors.lastname: Lawrence
authors.forename: P A
authors.initials: PA
authors.lastname: Edwards
authors.forename: K
authors.initials: K
authors.lastname: Simkiss
authors.forename: E A
authors.initials: EA
authors.lastname: Wider
mesh_terms: D000818:Animals; D001681:Biological Assay; D001711:Biotransformation; D049872:Bivalvia / Q000378:metabolism; D002250:Carbon Radioisotopes; D002733:Chlorophenols / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005618:Fresh Water / Q000737:chemistry*; D007448:Invertebrates / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009835:Oligochaeta / Q000378:metabolism; D010416:Pentachlorophenol / Q000378:metabolism; D010880:Piperidines / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism; D014274:Trifluralin / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D002733:Chlorophenols; D010880:Piperidines; D011721:Pyrenes; D014874:Water Pollutants, Chemical; C009534:2,4,5-trichlorophenol; C063198:fenpropidine; C030984:pyrene; D014274:Trifluralin; D010416:Pentachlorophenol; C004762:2,4-dichlorophenol