SH2-B is required for both male and female reproduction.
Many growth factors and hormones modulate the reproductive status in mammals. Among these, insulin and insulin-like growth factor I (IGF-I) regulate the development of gonadal tissues. SH2-B has been shown to interact with insulin and IGF-I receptors, although the role of SH2-B in these signals has not been clarified. To investigate the role of SH2-B, we generated mice with a targeted disruption of the SH2-B gene. Both male and female SH2-B(-/-) mice showed slight retardation in growth and impaired fertility. Female knockout mice possess small, anovulatory ovaries with reduced numbers of follicles and male SH2-B(-/-) mice have small testes with a reduced number of sperm. SH2-B(-/-) cumulus cells do not respond to either follicle-stimulating hormone or IGF-I. These data suggest that SH2-B plays a critical role in the IGF-I-mediated reproductive pathway in mice.
Ohtsuka
Satoshi
S
Division of Molecular Genetics, Institute of Life Science, Kurume University, Kurume 839-0861, USA.
Takaki
Satoshi
S
Iseki
Masanori
M
Miyoshi
Kanta
K
Nakagata
Naomi
N
Kataoka
Yuki
Y
Yoshida
Nobuaki
N
Takatsu
Kiyoshi
K
Yoshimura
Akihiko
A
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D007246:Infertility / Q000235:genetics* / Q000473:pathology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D012333:RNA, Messenger; C115327:Sh2bpsm1 protein, mouse; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone