title: Spitz/EGFr signalling via the Ras/MAPK pathway mediates the induction of bract cells in Drosophila legs.
abstract: In the development of Drosophila, the activation of the EGFr pathway elicits different cellular responses at different times and in different tissues. A variety of approaches have been used to identify the mechanisms that confer this response specificity. We have analysed the specification of bract cells in Drosophila legs. We observed that mechanosensory bristles induced bract fate in neighbouring epidermal cells, and that the RAS/MAPK pathway mediated this induction. We have identified Spitz and EGFr as the ligand and the receptor of this signalling, and by ubiquitous expression of constitutively activated forms of components of the pathway we have found that the acquisition of bract fate is temporally and spatially restricted. We have also studied the role of the poxn gene in the inhibition of bract induction in chemosensory bristles.
authors.affiliation: Centro de Biología Molecular Severo Ochoa-C.S.I.C., Facultad de Ciencias-CV, Universidad Autónoma-Cantoblanco, 28049 Madrid, Spain.
authors.forename: David
authors.initials: D
authors.lastname: del Alamo
authors.forename: Javier
authors.initials: J
authors.lastname: Terriente
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Díaz-Benjumea
mesh_terms: D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D004815:Epidermal Growth Factor; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics; D017344:Genes, Insect / Q000502:physiology; D020935:MAP Kinase Signaling System; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics; D051761:Paired Box Transcription Factors; D014157:Transcription Factors / Q000235:genetics; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D005136:Eye Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051761:Paired Box Transcription Factors; C073863:Poxn protein, Drosophila; D014157:Transcription Factors; C075638:aos protein, Drosophila; C076026:spi protein, Drosophila; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D020928:Mitogen-Activated Protein Kinases; D018631:ras Proteins