Colonization-resistant antimicrobial-coated peritoneal dialysis catheters: evaluation in a newly developed rat model of persistent Pseudomonas aeruginosa peritonitis.
OBJECTIVE
Development of a rat model of persistent peritonitis and evaluation of the ability of liposomal ciprofloxacin hydrogel-coated silicone to resist colonization.


METHODS
A newly developed model of persistent Pseudomonas aeruginosa peritonitis to compare the ability of liposomal ciprofloxacin hydrogel (LCH)-coated silicone versus plain silicone for resistance to bacterial colonization.


METHODS
Male Sprague-Dawley rats.


RESULTS
Inoculating the peritoneum of rats with 1 mL 0.5% agar containing 10(6) colony-forming units (cfu)/mL P. aeruginosa in the presence of a plain silicone coupon resulted in persistent peritonitis for at least 7 days. Plain silicone coupons in all 40 rats were colonized (median 2.54 x 10(3) cfu/cm2; range 5.0 x 10(1)-1.0 x 10(6) cfu/cm2) and peritoneal washings were consistently culture-positive. In contrast, the LCH coupons removed after 7 days from the 40 test rats were sterile, as were the peritoneal washings, and there was no evidence of peritonitis. Blood cultures were negative in both groups.


CONCLUSIONS
Liposomal ciprofloxacin hydrogel-coated silicone resists colonization in this rat model of persistent P. aeruginosa peritonitis.
Finelli
Anthony
A
Department of Surgery, University of Toronto, Ontario, Canada.
Burrows
Lori L
LL
DiCosmo
Frank A
FA
DiTizio
Valerio
V
Sinnadurai
Selva
S
Oreopoulos
Dimitrios G
DG
Khoury
Antoine E
AE
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D018441:Biofilms; D002408:Catheters, Indwelling; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004337:Drug Carriers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008081:Liposomes; D008297:Male; D010530:Peritoneal Dialysis / Q000009:adverse effects / Q000295:instrumentation*; D010538:Peritonitis / Q000209:etiology / Q000382:microbiology / Q000517:prevention & control*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012828:Silicones
D000890:Anti-Infective Agents; D004337:Drug Carriers; D008081:Liposomes; D012828:Silicones; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002939:Ciprofloxacin