Effects of neurotensin on the healing of experimental anastomosis of the colon.
The leakage of colonic anastomoses increases perioperative morbidity significantly. The purpose of the study was to investigate the influence of neurotensin, an intestinal trophic peptide, on the healing of colonic anastomosis. Forty-two Wistar-albino rats were separated into three equal groups: Group 1 (control group)--segmental resection of the left colon and end-to-end anastomosis; Group 2 (dexamethasone group)--resection and anastomosis, plus 0.1 mg/kg/day of dexamethasone; Group 3 (neurotensin group)--same surgical procedure plus 300 micrograms/kg/day of neurotensin. Bursting pressure and tissue hydroxyproline content were determined as parameters of the anastomosis strength and healing on the 3rd and 7th days postoperatively. On the 3rd day, mean bursting pressures were 141.4, 146.7 and 73.1 (p = 0.0001) cm of water in the control group, dexamethasone and neurotensin groups respectively. On the 7th day, bursting pressures were measured as 237.4, 100.6 (p = 0.0001) and 72.7 (p = 10(-6)) cm of water, in the control group, dexamethasone and neurotensin groups respectively. Between the 3rd and 7th days, bursting pressures were increased significantly in the control group (p = 0.0001), decreased in the dexamethasone (p = 0.048), and maintained their lowest values in the neurotensin (p = 0.96) groups. On the 7th day, mean hydroxyproline levels were measured as 9.20, 3.30 (p = 0.007), 2.86 (p = 0.007) micrograms, in the control group, dexamethasone, and neurotensin groups respectively. Between the 3rd and 7th days, tissue hydroxyproline levels were increased significantly in the control group (p = 0.004), decreased in the dexamethasone (p = 0.03), and maintained their lowest values in the neurotensin (p = 0.87) groups. The anastomosis resistance to intraluminal pressure was weak, tissue collagen content was insufficient, and healing was inadequate in the dexamethasone and neurotensin groups in respect to the control group. We concluded that neurotensin impaired the healing, and affected the strength of the colonic anastomosis.
Gürleyik
G
G
Department of Surgery, Haydarpasa Numune Hospital, Istanbul, Turkey. esg@e-kolay.net
Gürleyik
E
E
Yilmazcan
A
A
Ozcan
A
A
Onaran
I
I
Unalmiser
S
S
D000714:Anastomosis, Surgical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis; D003106:Colon / Q000601:surgery*; D003907:Dexamethasone / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009496:Neurotensin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence; D014945:Wound Healing / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D009496:Neurotensin; D003907:Dexamethasone; D003094:Collagen; D006909:Hydroxyproline