title: The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies.
abstract: The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells.
authors.affiliation: Section Yeast Genetics, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333AL, Leiden, The Netherlands.
authors.forename: Martijn J
authors.initials: MJ
authors.lastname: van Hemert
authors.forename: Gerda E M
authors.initials: GE
authors.lastname: Lamers
authors.forename: Dionne C G
authors.initials: DC
authors.lastname: Klein
authors.forename: Tjerk H
authors.initials: TH
authors.lastname: Oosterkamp
authors.forename: H Yde
authors.initials: HY
authors.lastname: Steensma
authors.forename: G Paul H
authors.initials: GP
authors.lastname: van Heusden
mesh_terms: D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis; D016366:Open Reading Frames; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D008941:Spindle Apparatus; D020798:Two-Hybrid System Techniques
chemical_list: D003598:Cytoskeletal Proteins; C456282:FIN1 protein, S cerevisiae; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins