A circular RNA-DNA enzyme obtained by in vitro selection.
A circular RNA-DNA enzyme with higher activity to target RNA cleavage and higher stability than that of the hammerhead ribozyme in the presence of RNase A was obtained by in vitro selection. The molecule is composed of a catalytic domain of 22-mer ribonucleotides derived from the hammerhead ribozyme and a fragment of 55-mer deoxyribonucleotides. The DNA fragment contains two substrate-binding domains (9-mer and 6-mer, respectively) and a "regulation domain" (assistant 40-mer DNA with 20-mer random deoxyribonucleotides sequence), which probably play the role in the regulation of flexibility and rigidity of the circular RNA-DNA enzyme. The above results suggest that the circular RNA-DNA enzyme will have a great prospect in gene-targeting therapies.
Kong
Xiang-duo
XD
Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, People's Republic of China.
Zhu
Shi-zhen
SZ
Gou
Xiao-jun
XJ
Wang
Xiao-ping
XP
Zhang
Hong-ying
HY
Zhang
Jin
J
D002384:Catalysis; D020650:Combinatorial Chemistry Techniques; D021881:DNA, Catalytic / Q000138:chemical synthesis* / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry; D004795:Enzyme Stability / Q000187:drug effects; D008274:Magnesium / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D016337:RNA, Catalytic / Q000138:chemical synthesis* / Q000737:chemistry*; D000079962:RNA, Circular; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000494:pharmacology
D021881:DNA, Catalytic; D004270:DNA, Circular; D016337:RNA, Catalytic; D000079962:RNA, Circular; D012313:RNA; D012259:Ribonuclease, Pancreatic; D008274:Magnesium