Scintigraphy of acute inflammatory lesions in rats with radiolabelled recombinant human neutrophil-activating peptide-2.
Radiolabelled recombinant human interleukin-8 (IL-8) with its homologue neutrophil-activating peptide-2 (NAP-2) have been compared for imaging acute sterile inflammatory lesions in rats. 125I-IL-8 and 125I-NAP-2 were prepared by reaction with chloramine-T and injected intravenously into male rats bearing subcutaneous carrageenan abscesses in their left hindlimbs. Left hindlimb and right hindlimb activities were determined from serial total-body scintigrams between 1 h and 96 h post-injection as regional per cent injected activity corrected for physical decay (%IA). Time-activity curves for 125I-IL-8 and 125I-NAP-2 in the carrageenan-containing left hindlimbs were similar in that both peaked at 1-3 h post-injection (IL-8, 4.9+/-0.5%IA; NAP-2, 4.8+/-1.9%IA) and decreased exponentially thereafter. However, while the lesioned-to-control limb activity ratio (L/C) for 125I-IL-8 only approximately doubled during the imaging period (1.7+/-0.3 at 1 h vs 3.7+/-1.0 at 24 h post-injection), L/C for 125I-NAP-2 more than tripled, rising from 1.5+/-0.4 at 1 h to 5.3+/-0.7 by 72 h post-injection. It is concluded that while both radiolabelled IL-8 and NAP-2 may prove useful for clinical imaging, radiolabelled NAP-2 may provide better discrimination of inflammatory lesions from normal tissue at later times post-injection.
Hay
R V
RV
Van Andel Research Institute, Grand Rapids, Michigan, USA.
Skinner
R S
RS
Newman
O C
OC
Kunkel
S L
SL
Lyle
L R
LR
Shapiro
B
B
Gross
M D
MD
D000818:Animals; D018925:Chemokines; D005260:Female; D006801:Humans; D007249:Inflammation / Q000000981:diagnostic imaging*; D016209:Interleukin-8; D007457:Iodine Radioisotopes; D008297:Male; D010455:Peptides; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D014018:Tissue Distribution; D001620:beta-Thromboglobulin
D018925:Chemokines; D016209:Interleukin-8; D007457:Iodine Radioisotopes; C061615:PPBP protein, human; D010455:Peptides; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D001620:beta-Thromboglobulin