title: A fluorescent anion sensor that works in neutral aqueous solution for bioanalytical application.
abstract: Anion recognition and anion sensing are of interest because anions play many important roles in living organisms. Most currently known anion sensors work only in organic solution, but sensors for biological applications are required to function in neutral aqueous solution. We have designed and synthesized a novel fluorescent sensor for anions. The sensor molecule 1-Cd(II) contains 7-amino-4-trifluoromethylcoumarin as a fluorescent reporter and Cd(II)-cyclen (1,4,7,10-tetraazacyclododecane) as an anion host. In neutral aqueous solution, Cd(II) of 1-Cd(II) is coordinated by the four nitrogen atoms of cyclen and the aromatic amino group of coumarin. When various anions are added to 100 mM HEPES buffer solution (pH 7.4) containing 1-Cd(II), the aromatic amino group of coumarin is displaced from Cd(II), causing a change of the excitation spectrum. While pyrophosphate and citrate were detected with high sensitivity, fluoride and perchlorate produced no response. Among organic anions, ATP and ADP gave strong signals, while cAMP showed little signal. By utilizing the different affinities of the sensor for AMP and cAMP, the activity of phosphodiesterase, which cleaves cyclic nucleotide, was monitored in real-time. The sensor should have many biochemical and analytical applications and the sensing principle should be widely applicable to the sensing of other molecules.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Mizukami
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Akira
authors.initials: A
authors.lastname: Odani
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kikuchi
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000032:analysis* / Q000378:metabolism; D000838:Anions / Q000032:analysis; D015374:Biosensing Techniques; D002104:Cadmium / Q000737:chemistry*; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009942:Organometallic Compounds / Q000737:chemistry*; D011199:Potentiometry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D000227:Adenine Nucleotides; D000838:Anions; D003374:Coumarins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D002104:Cadmium; D014867:Water; D015105:3',5'-Cyclic-AMP Phosphodiesterases