title: Induction of mucous cell metaplasia in the middle ear of rats using a three-step method: an improved model for otitis media with mucoid effusion.
abstract: Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10(7) colony-forming units (CFU)/ear or Haemophilus influenzae at 5 x 10(7) CFU/ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10(7) CFU/ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.
authors.affiliation: Department of Otolaryngology, University of Minnesota Otitis Media Research Center, University of Minnesota School of Medicine, Minneapolis, USA.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tsuboi
authors.forename: Youngki
authors.initials: Y
authors.lastname: Kim
authors.forename: G Scott
authors.initials: GS
authors.lastname: Giebink
authors.forename: Chap
authors.initials: C
authors.lastname: Le
authors.forename: Michael M
authors.initials: MM
authors.lastname: Paparella
authors.forename: Nengqian
authors.initials: N
authors.lastname: Chen
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Schachern
authors.forename: Steven K
authors.initials: SK
authors.lastname: Juhn
authors.forename: Jizhen
authors.initials: J
authors.lastname: Lin
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000473:pathology*; D005064:Eustachian Tube; D006801:Humans; D008679:Metaplasia; D009092:Mucous Membrane / Q000166:cytology / Q000473:pathology; D010034:Otitis Media with Effusion / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013296:Streptococcus pneumoniae; D013997:Time Factors