title: Validation of an analytical procedure for the determination of oxidative hair dyes in cosmetic formulations.
abstract: A high range and variety of cosmetic formulations that contain oxidative hair dyes and matrix-forming compounds have been industrially developed over recent years and are now available on the international market. Member states of the European Union are responsible for conducting analyses of cosmetic products as deemed necessary by law and European regulation enforcement. Therefore, inspection authorities as well as the cosmetics trade and industry need validated analytical methods for the identification, characterization, and/or quality control of specific active ingredients or formulations with the aim of implementing the European Union Cosmetic Directives (76/768/ECC, 95/17/EC). In this frame, we validated a candidate reference method that enables the identification and quantification of hair dye-forming compounds. This method consists of a separation by RP-HPLC coupled with a DAD after a liquid-liquid extraction procedure for separating matrix components from the dye-forming compounds. The validation of the method includes common criteria such as the repeatability of the analysis and the establishment of figures of merit, as well as statistical evaluations and quality assurance in order to follow the recommendations of the Eurachem guide for analytical measurements.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg, B-2240 Geel, Belgium.
authors.forename: Ursula
authors.initials: U
authors.lastname: Vincent
authors.forename: Guy
authors.initials: G
authors.lastname: Bordin
authors.forename: Adela R
authors.initials: AR
authors.lastname: Rodriguez
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003358:Cosmetics / Q000737:chemistry*; D004563:Electrochemistry; D006202:Hair Dyes / Q000032:analysis*; D010084:Oxidation-Reduction
chemical_list: D003358:Cosmetics; D006202:Hair Dyes