[Inhibition of glutamate uptake in rat brain synaptosome by methylmercury].
The effect of methylmercury on the glutamate uptake in rat brain synaptosomes were studied in vitro by using a high affinity uptake test. The result showed that methylmercury(1 x 10(-9) -1 x 10(-4) mol/L) could decrease 3H-L-glutamate uptake in rat cerebral cortex, hippocampus, striatum and cerebellum synaptosome by 0.7%-74.4%, 7.5%-91.1%, 1.4%-76.6% and 4.4%-77.0%, respectively, in a dose-dependent manner, and their IC50 were 7, 9, 3.6, 2.2 and 4.3 x 10(-6) mol/L, respectively. It suggested that methylmercury could obviously inhibit glutamate uptake in the synaptosomes of rat brain.
Zhu
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W
Department of Environmental Health, Tongji Medical University, Wuhan 430030, China.
Chen
X
X
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D008767:Methylmercury Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000378:metabolism
D008767:Methylmercury Compounds; D018698:Glutamic Acid