An in vitro screening method for DNA cytosine-C5-methylase inhibitor.
A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and lambda DNA as their substrate.
Tamura
Takashi
T
Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan. tktamura@cc.okayama-u.ac.jp
Kataoka
Atsushi
A
Shu
Li Yin
LY
Ashida
Akihiko
A
Tanaka
Hidehiko
H
Inagaki
Kenji
K
D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D001419:Bacteria; D004247:DNA / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors*; D015280:DNA Restriction-Modification Enzymes; D015257:DNA-Cytosine Methylases / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004791:Enzyme Inhibitors / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D012436:S-Adenosylmethionine / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome / Q000378:metabolism
D015280:DNA Restriction-Modification Enzymes; D004791:Enzyme Inhibitors; D012436:S-Adenosylmethionine; D004247:DNA; D015257:DNA-Cytosine Methylases; C027823:Cytosine 5-methyltransferase; D004248:DNA (Cytosine-5-)-Methyltransferases; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases; D000241:Adenosine; C006235:sinefungin