Rapid detection of neurotoxic insecticides in food using disposable acetyicholinesterase-biosensors and simple solvent extraction.
The extensive use of pesticides to protect agricultural crops necessitates reliable tools for the detection of residues in food and water, thus ensuring environmental protection and consumer safety. Neuroinhibitors such as organophosphates and carbamates in particular, represent a potential hazard to human health. These compounds are frequently found in food, but conventional methods of analysis are limited as they are either time consuming or not sufficiently sensitive. As a result, a rapid and sensitive biosensor test based on AChE-inhibition was developed. The disposable AChE-biosensor was directly applied in solvent extracts of food samples using isooctane as extraction solvent. A complete assay could be performed in less than 2 h. Recovery rates of 84% were obtained in tests with spiked orange juice samples. Tests in food samples with a lower water content resulted in reduced recovery rates (44% for peach pap baby food). Phosphorothionate insecticides in food could be detected after direct oxidation with N-bromosuccinimide and solvent extraction. The assay displayed a detection limit of 2 microg/kg paraoxon, which was sufficient for the monitoring of maximum residue limits in food according to EU regulations.
Schulze
Holger
H
Institute of Technical Biochemistry, University of Stuttgart, Germany.
Schmid
Rolf D
RD
Bachmann
Till T
TT
D000110:Acetylcholinesterase / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D004209:Disposable Equipment; D005506:Food Contamination / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D009946:Organothiophosphorus Compounds; D012680:Sensitivity and Specificity; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D000110:Acetylcholinesterase