[Assessment of p53 dependent apoptosis in glia-derived tumors of the brain].
There are conflicting reports in connection with the association of the p53 tumour suppressor gene mutation with the clinical and histopathological progression of gliomas. Glia-derived neoplasms frequently show mutational changes in the p53 gene which result in enhancement of tumorigenesis. The aim of the paper was an assessment of the frequency of mutations in the exon 8 of this gene. The specimens from 14 patients operated for glial tumors were investigated by polymerase chain reaction-assisted--single strand conformation polymorphism (PCR-SSCP). We found aberrant bands in 64.3% of specimens. The percentage of mutations was similar in patients with benign and malignant tumours. There was no correlation between the alteration of the gene and intensity of necrosis in histological examination in patients with glioblastoma. Changes in activity of the p53 gene were more frequent in younger patients and in males when compared to women.
Kaczmarczyk
R
R
Katedry i Kliniki Neurochirurgii i Neurochirurgii Dzieciecej AM w Lublinie.
Słodziński
H
H
Trojanowski
T
T
Polz-Dacewicz
M
M
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002648:Child; D005091:Exons; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012737:Sex Factors
Ocena zjawiska apoptozy w glejopochodnych guzach mózgu w oparciu o badanie antyonkogenu p53.