Assessment of tolerant sunfish populations (Lepomis sp.) inhabiting selenium-laden coal ash effluents. 2. Tissue biochemistry evaluation.
Sunfish were collected from a fly ash pond-receiving stream and an Ohio River reference site to assess biochemical responses to coal ash effluent exposure. Selenium levels in sunfish from the receiving stream were higher than toxic thresholds associated with adverse population effects and reproductive impairment. Tissue biochemistry was found to be indicative of metal exposure and effect, but varied widely. Liver glycogen was positively correlated with increased liver metal levels, indicating no adverse effect upon stored carbohydrate levels. Lipid levels decreased with increasing metals, indicating possible nutritional stress. Protein levels increased with increasing metal levels, possibly due to the synthesis of proteins to sequester the metals. ATPase, dUTPase, and alkaline phosphatase activity generally decreased with exposure to ash pond metals, but remained within normal physiological ranges. Fish condition factors and liver somatic indices were correlated with liver lipid levels, dUTPase activity, and gill ATPase and alkaline phosphatase activity. Exposure to coal ash effluents produced biochemical markers of exposure that were associated with fish condition indicators; however, the indices themselves were not significantly affected by effluent exposure.
Lohner
T W
TW
Environmental Services Department, American Electric Power, Columbus, Ohio 43215, USA. twlohner@aep.com
Reash
R J
RJ
Williams
M
M
D000222:Adaptation, Physiological; D000251:Adenosine Triphosphatases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003031:Coal; D004781:Environmental Exposure; D006003:Glycogen / Q000378:metabolism; D017745:Incineration; D050356:Lipid Metabolism; D008099:Liver / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D010473:Perciformes; D011506:Proteins / Q000032:analysis; D012037:Refuse Disposal; D012643:Selenium / Q000009:adverse effects* / Q000493:pharmacokinetics; D014873:Water Pollutants / Q000009:adverse effects* / Q000493:pharmacokinetics
D015415:Biomarkers; D003031:Coal; D011506:Proteins; D014873:Water Pollutants; D006003:Glycogen; D000251:Adenosine Triphosphatases; D012643:Selenium