title: Immunohistochemical distribution of epithelioid cell, myofibroblast, and transforming growth factor-beta1 in the granuloma caused by Mycobacterium avium intracellulare complex pulmonary infection.
abstract: The present study was designed to evaluate the distribution of epithelioid cells, myofibroblasts, and TGF-beta1 in the formation of granuloma caused by Mycobacterium avium intracellulare complex (MAC) lung infection. A retrospective study was performed for 9 cases of positive MAC culture in which lung resections were performed between January 1989 and August 1999. Resected lung specimens were evaluated histologically and immunohistochemically for CD68 (stain for monocytes and macrophages, and epithelioid cells) and alpha-smooth muscle actin as well as vimentin (stain for myofibroblasts), and TGF-beta1 was performed. When granuloma was initially formed, no myofibroblasts were found, but as caseous necrosis appeared, the thin epithelioid cell layer was detected and the outer myofibroblast layer gradually became thick. In the cavitary wall, the layer of epithelioid cells and multinucleated giant cells surrounded necrosis, and was associated with the outer layer of myofibroblasts. In addition, the anti-TGF-beta1 antibody stained the cytoplasm of epithelioid cells and multinucleated giant cells, preceding the advent of myofibroblasts. In summary, our present study evaluated distributions of epithelioid cells, myofibroblasts, and TGF-beta along with the morphogenesis of granuloma, and clearly demonstrated the immunohistochemical difference between granuloma with caseous necrosis and granulomas without caseous necrosis.
authors.affiliation: First Department of Internal Medicine, Kagawa Medical University, Japan. jiro@kms.ac.jp
authors.forename: Jiro
authors.initials: J
authors.lastname: Fujita
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: Ichizo
authors.initials: I
authors.lastname: Suemitsu
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yamadori
authors.forename: Eriko
authors.initials: E
authors.lastname: Shigeto
authors.forename: Masahiko
authors.initials: M
authors.lastname: Shiode
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Nishimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirayama
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Matsushima
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ishida
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015622:Epithelioid Cells / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006099:Granuloma / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D015270:Mycobacterium avium-intracellulare Infection / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D009336:Necrosis; D009784:Occupational Diseases / Q000473:pathology*; D010446:Peptide Fragments / Q000032:analysis; D011013:Pneumonectomy; D012189:Retrospective Studies; D016212:Transforming Growth Factor beta / Q000032:analysis*; D053773:Transforming Growth Factor beta1; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000473:pathology* / Q000601:surgery; D014746:Vimentin / Q000032:analysis; D000097382:CD68 Molecule
chemical_list: D000199:Actins; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D010446:Peptide Fragments; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014746:Vimentin; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C508960:TGFB1 protein, human; C066950:vimentin (1-96)