alpha-Crystallin chaperone function in diabetic rat and human lenses.
This study focussed on the effect of diabetes on the chaperone function of alpha-crystallin. The authors relied on diabetic rats with a wide range of plasma glucose levels and non-diabetic control rats to establish a possible relationship between severity of diabetes and alpha-crystallin chaperone activity. In addition, 52-56 and 63-69 year-old diabetic and non-diabetic human lenses were used to show whether diabetes affects alpha-crystallin chaperone activity in human lenses. Correlation between plasma glucose levels and loss of chaperone activity of the alphaL-crystallin fraction in diabetic rats indicated good correlation. The glycemic threshold, reported before for cataract development in diabetic rats, seems to be valid for the chaperone activity loss as well. Analysis of the human lens alphaL-crystallin showed lower chaperone activity in all the diabetic lenses than in the age-matched control lenses. In the 63-69 age group, the loss in chaperone activity due to diabetes was significantly larger than in the 52-56 age group suggesting a dominant effect of duration of diabetes.
Thampi
Prajitha
P
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Zarina
Shamshad
S
Abraham
Edathara C
EC
D000368:Aged; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D016022:Case-Control Studies; D003459:Crystallins / Q000502:physiology*; D003920:Diabetes Mellitus / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D006801:Humans; D007908:Lens, Crystalline / Q000502:physiology*; D008875:Middle Aged; D018832:Molecular Chaperones / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D003459:Crystallins; D018832:Molecular Chaperones; D000426:Alcohol Dehydrogenase