[Analysis of P/N leak subtraction of pClamp acquisition software].
Using blind whole cell recording technique on rat hippocampal slices, the function and mechanisms of P/N leak subtraction of Clampex program in pClamp acquisition software were studied. The ways of picking up sub-pulse current and properly selecting parameters, including time course, polarization, sub-holding potential, numbers and location of P/N sub-pulse were analyzed in detail. Our results indicate that voltage-gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction.
Liu
Z W
ZW
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
Li
L J
LJ
Liu
C G
CG
D000818:Animals; D006624:Hippocampus / Q000502:physiology; D007473:Ion Channels; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D012984:Software
D007473:Ion Channels; D024642:Potassium Channels, Voltage-Gated