UV-irradiated DNA matrixes selectively bind endocrine disruptors with a planar structure.
DNA has unique chemical properties and is readily purified from salmon milts and shellfish gonads. DNA has few commercial uses and is generally discarded as an industrial waste. Recently, we prepared water-insoluble and nuclease-resistant DNA-films by UV irradiation. The DNA-films removed DNA-intercalating compounds. Here, we immobilized double-stranded DNA onto porous glass beads by UV irradiation and prepared DNA-immobilized glass bead columns. The DNA-immobilized columns effectively accumulated more DNA-intercalating materials than the DNA-films. The DNA-immobilized columns bound endocrine disruptors with a planar structure, such as dioxin-derivatives, polychlorobiphenyl (PCB)-derivatives, and benzo[a]pyrene. Bisphenol A and diethylstilbestrol, which lack a planar structure, did not bind to the DNA-immobilized columns. These results suggest that DNA-immobilized glass bead columns could selectively remove chemical compounds with a planar structure by intercalation into the double-stranded DNA. DNA-immobilized glass beads and DNA-films prepared by UV irradiation have the potential to serve as useful biomaterials for medical, engineering, and environmental applications.
Yamada
Masanori
M
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Kato
Kozue
K
Nomizu
Motoyoshi
M
Ohkawa
Kousaku
K
Yamamoto
Hiroyuki
H
Nishi
Norio
N
D001665:Binding Sites; D001681:Biological Assay; D004247:DNA; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D005898:Glass; D015394:Molecular Structure; D014466:Ultraviolet Rays
D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D004247:DNA