Identification of novel ethanol-sensitive genes by expression profiling.
Chronic exposure to ethanol or other addicting drugs causes long-lasting, deleterious behavioral responses, such as tolerance, dependence, sensitization, and addiction. Changes in brain gene expression are thought to be a critical component of these behavioral adaptations. Our laboratory and others have utilized cultured neuronal cells as model systems for studying gene regulation by ethanol. Recently, the use of non-biased, high-throughput approaches to studying gene expression has allowed identification of gene regulation "patterns," rather than single genes responding to ethanol. This review will discuss how expression-profiling approaches can be used to identify functional changes occurring in neural cells with chronic exposure to ethanol.
Rahman
S
S
The Ernest Gallo Clinic and Research Center and the Department of Neurology, University of California at San Francisco, 5858 Horton Street, Suite 200, Emeryville, CA 94608, USA.
Miles
M F
MF
D002492:Central Nervous System Depressants / Q000009:adverse effects / Q000494:pharmacology*; D000431:Ethanol / Q000009:adverse effects / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
D002492:Central Nervous System Depressants; D000431:Ethanol