title: Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli.
abstract: The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.
authors.affiliation: Institute of Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan.
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Lin
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Yeh
authors.forename: Chan-Yi
authors.initials: CY
authors.lastname: Yang
authors.forename: Chan-Long
authors.initials: CL
authors.lastname: Chen
authors.forename: Gee-Fong
authors.initials: GF
authors.lastname: Chen
authors.forename: Chia-Chun
authors.initials: CC
authors.lastname: Chen
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Wu
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism; D018830:Adhesins, Escherichia coli; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D006046:Gold; D008358:Mannose