title: The effect of Brucella abortus on hydrogen peroxide and nitric oxide production by bovine polymorphonuclear cells.
abstract: The effect of Brucella on the generation of microbicidal reactive oxygen and nitrogen metabolites by bovine peripheral polymorphonuclear cells (PMNs) was investigated. The PMNs were recovered from the peripheral blood of control calves and experimental calves previously vaccinated against brucellosis. Significantly larger quantities of NO and H2O2 were generated by PMNs from control and experimental calves following activation by heat-killed whole cells or outer membrane protein of Brucella abortus than by non-activated cells (p<0.05-0.01). In contrast, generation of H2O2 and NO decreased when PMNs were exposed to the lipopolysaccharide of Brucella. However, the generation of H2O2 and NO by activated PMNs from the control and experimental calves did not differ significantly.
authors.affiliation: FAO/WHO Collaborating Center for Research and Training in Veterinary Public Health, Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar.
authors.forename: L
authors.initials: L
authors.lastname: Iyankan
authors.forename: D K
authors.initials: DK
authors.lastname: Singh
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002004:Brucella Vaccine / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000097:blood / Q000276:immunology / Q000382:microbiology; D002417:Cattle / Q000097:blood / Q000276:immunology* / Q000382:microbiology*; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D014611:Vaccination
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002004:Brucella Vaccine; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide