title: Separation in slalom chromatography: stretching and velocity dependence.
abstract: Slalom chromatography (SC) is used for the separation of large double-stranded DNA molecules. In this technique, the progression of the DNA fragments through the closed column packing follows the flow direction and is like a snake edging is way into long grass. A novel mathematical model is developed in this paper to describe this hydrodynamic phenomenon. The results obtained provided a model for the resolution between two adjacent peaks on a chromatogram. As well, a chromatographic response function was used to obtain the most efficient separation conditions for a mixture of DNA fragments with sizes higher than 15 kbp in a minimum analysis time.
authors.affiliation: Laboratoire de Chimie Analytique, Faculté de Médecine-Pharmacie, Université de Besancon, Besançon, France.
authors.forename: Yves Claude
authors.initials: YC
authors.lastname: Guillaume
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Perrin
authors.forename: Christiane
authors.initials: C
authors.lastname: Guinchard
authors.forename: Laurence
authors.initials: L
authors.lastname: Nicod
authors.forename: Tong Thanh
authors.initials: TT
authors.lastname: Truong
authors.forename: Alain
authors.initials: A
authors.lastname: Xicluna
authors.forename: Joelle
authors.initials: J
authors.lastname: Millet
authors.forename: Mireille
authors.initials: M
authors.lastname: Thomassint