title: [Fluorescence probe two-hybridization technique and its application in medical genechips].
abstract: OBJECTIVE
  To establish a new nucleic acid hybridization detection technique which may be used in medical genechips.
  
  
  METHODS
  The specific DNA fragment was detected by sequential two hybridization of fluorescence probe with template DNA and fixed DNA probe.
  
  
  RESULTS
  Fluorescence probe two-hybridization (FPTH) was applied to genechips for the detection of sex-transmitted pathogens from culture strains, and the results showed that the values of fluorescence density of the positive groups decreased remarkably when compared with those of the negative group. Both the sensitivity and specificity for detecting clinical samples are higher than 90%. There is no need of any additional reagent in hybridization procedure, and the hybridization detection can be accomplished in 40 minutes.
  
  
  CONCLUSIONS
  The FPTH technique is rapid, simple and reliable, it can also make the clinical detection process completely automatic and integrative.
authors.affiliation: Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041 P. R. China. szzhang@mcwcums.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Yong
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Xia
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016990:Ureaplasma urealyticum / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D005456:Fluorescent Dyes