Bacterial removal of quinolizidine alkaloids and other carbon sources from a Lupinus albus aqueous extract.
Two Gram-negative bacterial strains capable of using lupanine, the predominant quinolizidine alkaloid in Lupinus albus, as a sole carbon source were isolated from soil in which L. albus and L. luteus had been grown [Santana, F. M. et al. J. Ind. Microbiol. 1996, 17, 110-115]. In the present study, we present results suggesting that these isolates are of potential interest for removing lupanine and other quinolizidine alkaloids (QA) from the effluent resulting from the wet processing of Lupinus seeds, at temperatures within the range 20-34 degrees C. Growth in L. albus aqueous extract was diauxic, with a first period of rapid growth leading to the simultaneous consumption of a significant part of the initial concentration of QA (3 g L(-1), being 2 g L(-1) lupanine) and amino acids (1.5 g L(-1)). This period was followed by a second period of slower growth corresponding to the subsequent partial utilization (25%) of the carbohydrates (initial concentration of 20 g L(-1)) together with further removal of QA and amino acids. Despite the differences detected in the susceptibility of the two strains to lupanine toxicity, in particular at supraoptimal temperatures, and in the efficiency of lupanine catabolism, their performance on L. albus extract did not vary significantly.
Santana
Filomena M C
FM
Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
Pinto
Teresa
T
Fialho
Arsénio M
AM
Sá-Correia
Isabel
I
Empis
José M A
JM
D000470:Alkaloids / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D007887:Fabaceae / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry*; D011807:Quinolizines / Q000378:metabolism*; D013034:Sparteine / Q000031:analogs & derivatives
D000470:Alkaloids; D010936:Plant Extracts; D011807:Quinolizines; C008298:lupanine; D013034:Sparteine; D002244:Carbon