A linear plasmid temperature-sensitive for replication in Streptomyces hygroscopicus 10-22.
Streptomyces hygroscopicus 10-22 harbors a conjugative, autonomously replicating linear plasmid pHZ6 of ca. 70 kb, which shows no obvious homology with chromosomal DNA and is temperature-sensitive for replication, being stable in the host at 28 degrees C but easily lost at 37 degrees C. On a lawn of the wild-type S. hygroscopicus 10-22 cured of pHZ6, pHZ6 elicit pocks. Temperature sensitivity seemed to be a unique property for pHZ6 among six linear plasmids tested, including the well-known linear plasmids SLP2 in Streptomyces lividans 1326 and SCP1 in Streptomyces coelicolor A3(2). The distinct identity of pHZ6 from previously identified pHZ1-pHZ5 was demonstrated by the profile of relevant plasmids in six well-defined strains originated from S. hygroscopicus 10-22.
Pang
Xiuhua
X
Bio-X Life Science Research Center, Shanghai Jiaotong University, 200030, Shanghai, PR China.
Sun
Yuhui
Y
Liu
Jun
J
Zhou
Xiufen
X
Deng
Zixin
Z
D003470:Culture Media; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial