title: Azelastine and suplatast shorten the distribution half-life of IgE in rats.
abstract: We aim to clarify whether suplatast and azelastine (anti-allergic drugs) can shorten the half-life of imnunoglobulin E (IgE) in the circulating blood. Thirty Wistar rats were divided into six groups. Distilled water or anti-allergic drugs were given orally for 6 days after the first sensitization. Two milligrams of monoclonal dinitrophenyl (DNP)-specific rat IgE was administered to the rats, which had been given suplatast or azelastine orally. The level of DNP-specific rat IgE in the serum was estimated by IgE-capture enzyme-linked immunosorbent assay, and the turnover of IgE was analyzed from its pharmacokinetic parameters. The elimination half-life of rat IgE was about 12 h irrespective of the sensitized state. The intercompartmental rate constants (Kct and Ktc) in the suplatast-administered or azelastine-administered group were larger than those of the distilled water-administered group under non-sensitized conditions. These findings suggested that the anti-allergic drugs used in the present study facilitated the excretion of IgE from the circulation in rats.
authors.affiliation: 1st Department of Physiology, School of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hanashiro
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Tokeshi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nakasone
authors.forename: Masanori
authors.initials: M
authors.lastname: Sunagawa
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Kosugi
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001190:Arylsulfonates / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006207:Half-Life; D007073:Immunoglobulin E / Q000378:metabolism*; D008297:Male; D010793:Phthalazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013452:Sulfonium Compounds / Q000494:pharmacology*
chemical_list: D018926:Anti-Allergic Agents; D001190:Arylsulfonates; D010793:Phthalazines; D013452:Sulfonium Compounds; D007073:Immunoglobulin E; C073028:suplatast tosilate; C020976:azelastine