Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
Mutations arising in Ig V genes during an immune response are most likely introduced by one or several error-prone DNA polymerases. Many of the recently described nonreplicative DNA polymerases have an intrinsic fidelity compatible with such an activity, the strongest candidates being polymerase (pol) eta, pol iota, pol zeta, and pol mu. We report in this work that mice inactivated for either of the two polymerases related to pol beta (i.e., pol mu and pol lambda) are viable and fertile and display a normal hypermutation pattern.
Bertocci
Barbara
B
Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker-Enfants Malades, Paris, France.
De Smet
Annie
A
Flatter
Eric
E
Dahan
Auriel
A
Bories
Jean-Christophe
JC
Landreau
Catherine
C
Weill
Jean-Claude
JC
Reynaud
Claude-Agnès
CA
D000818:Animals; D019951:DNA Polymerase beta / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D004259:DNA-Directed DNA Polymerase / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D020868:Gene Silencing / Q000276:immunology; D005803:Genes, Immunoglobulin / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation / Q000276:immunology*
C415367:DNA polymerase beta2; C407743:DNA polymerase mu; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase