Cloning and tissue distribution of three murine alpha/beta hydrolase fold protein cDNAs.
We have cloned 3 novel murine cDNAs encoding proteins containing an alpha/beta hydrolase fold; a catalytic domain found in a very wide range of enzymes. These proteins belong to the prosite UPF0017 uncharacterized protein family and we have named them lung alpha/beta hydrolase 1, 2, and 3 (LABH) since they were cloned from lung cDNA. All have 9 coding exons, encoding 412, 425, and 411 residue proteins respectively (46-48 kDa); LABH1 being closely related to LABH3 having 45% identity. All 3 proteins have a single predicted amino-terminus transmembrane domain. An alignment of family members from different phyla enabled the identification of the LABH1 catalytic triad as Ser211, Asp337, and His366. mRNA expression levels of LABH1 and 3 were highest in liver and LABH2 highest in testis. These findings suggest that the LABH proteins consist of a novel family of membrane bound enzymes whose function has yet to be determined.
Edgar
Alasdair J
AJ
Tissue Engineering Centre, Division of Investigative Science, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Chelsea & Westminster Hospital, 369 Fulham Road, London SW10 9NH, United Kingdom. alasdair.edgar@ic.ac.uk
Polak
Julia M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D018076:DNA, Complementary; D004646:Emphysema / Q000201:enzymology / Q000235:genetics; D006801:Humans; D006867:Hydrolases / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D054467:Phospholipases A2; D010802:Phylogeny; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013737:Testis / Q000201:enzymology; D014018:Tissue Distribution
D018076:DNA, Complementary; D008565:Membrane Proteins; D006867:Hydrolases; C454239:LABH1, mouse; C454240:Abhd2 protein, mouse; C454241:Abhd3 protein, mouse; D054467:Phospholipases A2