title: Mass spectrometric analysis of cyclofenil and its metabolites in human urine.
abstract: Using gas chromatography/electron impact-mass spectrometry (GC/EI-MS) and high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS), the structures of cyclofenil metabolites in human urine have been assigned. The hydroxyl metabolites liberated from the glucuronide conjugates after acid hydrolysis were characterized as the trimethylsilyl (O-TMS) derivatives using GC/MS. The conjugate glucuronide forms were detected without hydrolysis by HPLC/MS. Cyclofenil was not observed in urine. Tentative structures for the two metabolites are proposed.
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, P.O. Box 131, Seoul 136-791, Korea. swmyung@kist.re.kr
authors.forename: Seung-Woon
authors.initials: SW
authors.lastname: Myung
authors.forename: Hye-Ki
authors.initials: HK
authors.lastname: Min
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Myungsoo
authors.initials: M
authors.lastname: Kim
authors.forename: SuJin
authors.initials: S
authors.lastname: Cha
authors.forename: Eun-Ah
authors.initials: EA
authors.lastname: Yoo
authors.forename: Yong-Hyeon
authors.initials: YH
authors.lastname: Yim
mesh_terms: D000328:Adult; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003506:Cyclofenil / Q000652:urine*; D005260:Female; D005300:Fertility Agents, Female / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male
chemical_list: D005300:Fertility Agents, Female; D003506:Cyclofenil