In vivo and in vitro effects of bromelain on PGE(2) and SP concentrations in the inflammatory exudate in rats.
The effects of bromelain were examined in rats with subcutaneous carrageenin-induced inflammation. After oral in vivo administration, bromelain (10 and 20 mg/kg p.o.) induced a significant decrease of both PGE(2) and substance P concentrations in the exudate. When added to the inflammatory exudate in vitro, the drug (25, 50, 100 microg/ml) did not affect PGE(2) concentrations and induced an increase in the substance P levels. Our data indicate that bromelain reduces the production of two key mediators of inflammation. This effect does not seem to be related to a direct action of the drug on PGE(2) and SP released in the exudate in response to the inflammatory stimulus.
Gaspani
Leda
L
Department of Pharmacology, University of Milan, Italy.
Limiroli
Elena
E
Ferrario
Paolo
P
Bianchi
Mauro
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001963:Bromelains / Q000494:pharmacology*; D002351:Carrageenan; D015232:Dinoprostone / Q000378:metabolism*; D005122:Exudates and Transudates / Q000187:drug effects / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013373:Substance P; D002351:Carrageenan; D001963:Bromelains; D015232:Dinoprostone