title: A single amino acid deletion in the antigen binding site of BoLA-DRB3 is predicted to affect peptide binding.
abstract: Two bovine MHC class II alleles, BoLA-DRB3*0201 and BoLA-DRB3*3301, contain a three base pair deletion which results in the deletion of a lysine (K beta 65) in the antigen recognition site (ARS). Modelling of BoLA-DRB3*0201 with the conserved lysine K beta 65 and BoLA-DRB3*0201 without K beta 65 indicated that this deletion altered the peptide specificity of the ARS, and may impact on the immune response. To test this hypothesis, the presence of K beta 65 was analysed in a sample of cattle vaccinated with the commercial cattle tick vaccine (TickGARD). Homozygous deletion of K beta 65 was significantly associated with high response to TickGARD (P<0.05). Screening of the TickGARD antigen identified a potential T cell epitope that is recognised better by animals that are homozygous for the K beta 65 deletion. This study provides evidence that changes in the ARS of MHC class II molecules may be associated with the well recognised animal to animal variation in magnitude of vaccine response.
authors.affiliation: Co-operative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, Qld 4029, Australia. sittek@cryptic.rch.unimelb.edu.au
authors.forename: Karin
authors.initials: K
authors.lastname: Sitte
authors.forename: Ross
authors.initials: R
authors.lastname: Brinkworth
authors.forename: Iain J
authors.initials: IJ
authors.lastname: East
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Jazwinska
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D005802:Genes, MHC Class II / Q000235:genetics / Q000276:immunology*; D008958:Models, Molecular; D009154:Mutation / Q000276:immunology / Q000502:physiology; D011485:Protein Binding; D011487:Protein Conformation; D013984:Tick Infestations / Q000517:prevention & control; D013987:Ticks / Q000276:immunology*; D014612:Vaccines / Q000276:immunology* / Q000592:standards
chemical_list: D000906:Antibodies; D000941:Antigens; D018984:Epitopes, T-Lymphocyte; D014612:Vaccines