title: Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line.
abstract: GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.
authors.affiliation: Department of Physiology, School of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Gil-Puig
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: T
authors.initials: T
authors.lastname: García-Caballero
authors.forename: C
authors.initials: C
authors.lastname: Segura
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Fernández
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry*; D015153:Blotting, Western / Q000379:methods; D001943:Breast Neoplasms / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D013006:Growth Hormone / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger; D013006:Growth Hormone