title: Evaluation of a porcine circovirus type 2-specific antigen-capture enzyme-linked immunosorbent assay for the diagnosis of postweaning multisystemic wasting syndrome in pigs: comparison with virus isolation, immunohistochemistry, and the polymerase chain reaction.
abstract: Quantitative virus isolation, immunohistochemistry, polymerase chain reaction (PCR) assay, and a porcine circovirus 2 (PCV2)-specific antigen-capture enzyme-linked immunosorbent assay (ELISA) were used for differentiation between clinical and subclinical PCV2 infections of swine. Tissue samples from pigs experimentally infected with PCV2 and field cases of postweaning multisystemic wasting syndrome and PCV2-associated reproductive disorders were used in this evaluation. In initial studies on 6 PCV2 pools using 3 previously published PCR protocols for PCV2 detection, quantitative virus isolation, and antigen-capture ELISA, substantial differences in sensitivity were identified among these procedures. Examination of tissue samples from diseased and clinically normal pigs indicated that immunohistochemistry, quantitative virus isolation, and antigen-capture ELISA could be used to differentiate between clinical and subclinical PCV2 infections, but the PCR assay could not. Because subclinical infections of pigs with PCV2 are common, the use of nonquantitative PCR as a diagnostic tool for PCV2-related diseases should be discouraged and the PCV2-specific antigen-capture ELISA evaluated further.
authors.affiliation: Department of Agriculture and Rural Development for Northern Ireland, Veterinary Sciences Division, Stormont, Belfast.
authors.forename: F
authors.initials: F
authors.lastname: McNeilly
authors.forename: I
authors.initials: I
authors.lastname: McNair
authors.forename: M
authors.initials: M
authors.lastname: O'Connor
authors.forename: S
authors.initials: S
authors.lastname: Brockbank
authors.forename: D
authors.initials: D
authors.lastname: Gilpin
authors.forename: C
authors.initials: C
authors.lastname: Lasagna
authors.forename: G
authors.initials: G
authors.lastname: Boriosi
authors.forename: B
authors.initials: B
authors.lastname: Meehan
authors.forename: J
authors.initials: J
authors.lastname: Ellis
authors.forename: S
authors.initials: S
authors.lastname: Krakowka
authors.forename: G M
authors.initials: GM
authors.lastname: Allan
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D017925:Circovirus / Q000276:immunology* / Q000472:pathogenicity; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards* / Q000662:veterinary; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D019282:Wasting Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D014886:Weaning
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral