The terminal cis-glycol-group of phenylalanine specific tRNA (tRNA(Phe), I) from yeast was oxidized to the dialdehyde by NaIO(4) (tRNA(Phe)(oxi), II) and subsequently reduced to the diol by NaBH(4) (tRNA(Phe)(oxi-red), III). In charging experiments it could be shown that tRNA(Phe)(oxi-red) is still completely specific for phenylalanine. The Michaelis constant of tRNA(Phe)(oxi-red) remains unchanged when compared with tRNA(Phe), while the maximun velocity of charging upsilon(max) of tRNA(Phe)(oxi-red) is decreased to about one half.
Cramer
F
F
Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie, 3400, Göttingen, Germany
Haar
F v.d.
F
Schlimme
E
E
Uber das aminoacylierungsverhalten chemisch modifizierter phenylalaninspezifischer transfer-ribonucleinsäure aus hefe: (1) Glykolspaltung und reduktion zum diol an der 3'-terminalen ribose.