Solenin, a novel protein with translation-inhibiting activity from the traditional Chinese medicinal fish, the sea dragon Solenognathus hardwickii.
A single-chained protein designated solenin was isolated from Solenognathus hardwickii, a fish used as traditional Chinese medicinal material. Solenin was capable of inhibiting translation in a cell-free rabbit reticulocyte lysate system with an IC(50) of 2 microM and expressing a ribonuclease activity of 0.8U/mg toward yeast transfer RNA, but it lacked N-glycosidase activity characteristic of ribosome inactivating proteins Solenin exhibited a molecular weight of 18kDa and possessed an N-terminal sequence AHDAEVNEVKAQVAA. The protein was adsorbed on three types of chromatographic media: Affi-gel blue gel, CM-Sepharose and Mono S. It was devoid of antifungal and lectin activities.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. biochemistry@cuhk.edu.hk
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D000595:Amino Acid Sequence; D000818:Animals; D002474:Cell-Free System; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins; D005399:Fishes; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014176:Protein Biosynthesis / Q000187:drug effects*; D011506:Proteins; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D029941:Fish Proteins; D011506:Proteins; C464759:solenin protein, Solenognathus hardwickii; D012260:Ribonucleases