[Cloning of the human cytochrome p450 2B6 gene in Escherichia coli cells].
Human cytochrome P450 2B6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+)/CYP 2B6 was expressed in E. coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium.
Kolomeĭchuk
S N
SN
Orekhovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow 119992.
Pokrovskaia
M V
MV
Borisova
A A
AA
Zhgun
A A
AA
Solodar'
L I
LI
Sokolov
N N
NN
Shvets
V I
VI
Gervaziev
Iu V
IuV
El'darov
M A
MA
Archakov
A I
AI
D001189:Aryl Hydrocarbon Hydroxylases; D003001:Cloning, Molecular; D065702:Cytochrome P-450 CYP2B6; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D006801:Humans; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6; D010089:Oxidoreductases, N-Demethylating
Klonirovanie gena tsitokhroma p450 2B6 cheloveka v kletkakh Escherichia coli.