title: Experiments with micro-organisms and human cell cultures in the Zond 5 and Zond 7 flights.
abstract: Lysogenic strains of Escherichia coli were exposed to space conditions aboard the flight of Zond 5 and Zond 7. Space flight factors appeared to affect the state of episome systems of bacteria, as judged by data obtained with F-Lac+ donor cells which also carried genetic markers for threonine and leucine. Observations on phage induction are discussed and compared with results obtained aboard Biosatellite 2. A number of monolayer cultures of human cells (HeLa cells, fibroblasts, and A-1 cells) were repeatedly exposed to the space environment. In one instance, HeLa 19 cells increased in size after exposure to space conditions, a change which appeared to be genetically stable. HeLa 19 cells which were carried on six separate space flights showed a higher viability than corresponding cultures which were exposed only once aboard Zond 5.
authors.affiliation: USSR.
authors.forename: N N
authors.initials: NN
authors.lastname: Zhukov-Verezhnikov
authors.forename: M N
authors.initials: MN
authors.lastname: Volkov
authors.forename: I N
authors.initials: IN
authors.lastname: Maisky
authors.forename: N I
authors.initials: NI
authors.lastname: Rybakov
authors.forename: M A
authors.initials: MA
authors.lastname: Guberniev
authors.forename: I I
authors.initials: II
authors.lastname: Podoplelov
authors.forename: A N
authors.initials: AN
authors.lastname: Kulagin
authors.forename: E D
authors.initials: ED
authors.lastname: Aniskin
authors.forename: K D
authors.initials: KD
authors.lastname: Rybakova
authors.forename: N I
authors.initials: NI
authors.lastname: Sharyi
authors.forename: I P
authors.initials: IP
authors.lastname: Voronkova
authors.forename: P P
authors.initials: PP
authors.lastname: Saxonov
authors.forename: V Y
authors.initials: VY
authors.lastname: Kopyev
authors.forename: V V
authors.initials: VV
authors.lastname: Antipov
authors.forename: V A
authors.initials: VA
authors.lastname: Kozlov
authors.forename: G P
authors.initials: GP
authors.lastname: Parfyonov
authors.forename: V I
authors.initials: VI
authors.lastname: Orlovsky
mesh_terms: D002468:Cell Physiological Phenomena; D048429:Cell Size; D002478:Cells, Cultured; D003359:Cosmic Radiation; D004926:Escherichia coli; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D008940:Mitotic Index; D010957:Plasmids / Q000235:genetics / Q000502:physiology*; D013026:Space Flight; D014775:Virus Activation / Q000235:genetics / Q000502:physiology*; D014893:Weightlessness