[The polymorphic study of HLA-DRB1 gene in Chinese Liaoning Han with PCR-sequence specific primers].
8 allele and group specificity at HLA-DRB1 locus were genotyped in 159 unrelated individuals of Liaoning Han, with polymerase chain reaction-sequence specific primers. The range of gene frequencies was from 0.02201-0.23899. Heterozagosity was 83%. 33 out of 36 theoretical genotypes were identified and the result conformed to Hardy-Weinberg equilibrium law. The present method can be used not only in paternity test, individual identification, but also in clinic graft matching between donor and recipient as well as the study of HLA.
Liu
L
L
Department of Forensic Medicine, China Medical University, Shenyang 110001.
Hu
S
S
Jia
J
J
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China; D005006:Ethnicity; D005260:Female; D005787:Gene Frequency; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006579:Heterozygote; D006801:Humans; D008297:Male; D010334:Paternity; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains