[The effects of C2 gene on growth of gastric cancer cells].
OBJECTIVE
To investigate the effect of a newly cloned full length gene, C2 gene, which encodes human eukaryotic translation initiation factor, on growth of gastric cancer cells.


METHODS
A constructed eukaryotic vector carrying the full length of C2 gene was amplified, purified and transfected into gastric cancer cell line-SGC 7901. Expression of C2 protein was examined with fluorescence activated cell sorting (FACS) and immunohistochemical staining. Growth curve of the C2 gene transfected gastric cancer cells was drawn with the results of MTT assay and cell count.


RESULTS
FACS and immunohistochemical staining showed that C2 protein was expressed highly and stably in the C2 gene transfected cells. The growth curve of the C2 gene transfected cells by both MTT and cell count showed that the C2 gene transfected cells had less proliferation than the control cells. The growth cycle of transfected cells was 11 days, while the control was 7 days.


CONCLUSIONS
C2 gene transfection may inhibit the growth of human gastric cancer cells. It might become a potential inhibitor of gastric cancer in the level of protein translation initiation.
Chen
Caiping
C
Department of Gastroenterology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Liu
Jie
J
Yin
Fang
F
Zhang
Qing
Q
Wu
Kaichun
K
Fan
Daiming
D
D002455:Cell Division; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D005434:Flow Cytometry / Q000379:methods; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D010448:Peptide Initiation Factors / Q000235:genetics / Q000502:physiology*; D013274:Stomach Neoplasms / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
C462544:C2 translation initiation factor, human; D010448:Peptide Initiation Factors; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII