Solubilized DNA-dependent RNA polymerase from human placenta: A Mn(2+)-dependent enzyme.
The 100-fold purified RNA polymerase activity from human placenta is completely dependent upon added DNA. The enzyme is most active at 3 mM Mn(2+) in the presence of 100 mM (NH(4))(2)SO(4). Denatured DNA is a better template than native DNA. alpha-Amanitin completely inhibits the incorporation of 3H-UMP, while rifampicin has no influence upon the enzymatic activity.
Voigt
H -P.
H
Arbeitsgruppe Biochemie, Max-Planck-Institut für experimentelle Medizin, 34, Göttingen, Germany
Kaufmann
R
R
Matthaei
H
H