title: Analysis of tofisopam in human serum by column-switching semi-micro high-performance liquid chromatography and evaluation of tofisopam bioequivalency.
abstract: A rapid and sensitive column-switching semi-micro HPLC method is described for the direct analysis of tofisopam in human serum. The sample (100 microL) was directly injected onto the precolumn (Capcell Pak MF Ph-1), where unretained proteins were eluted to waste. Tofisopam was then eluted into an enrichment column using 13% acetonitrile in 50 mM phosphate buffer (pH 7.0) containing 5 mM sodium octanesulfonate and subsequently into the analytical column using 43% acetonitrile in 0.1% phosphoric acid containing 5 mM sodium octanesulfonate. The detection limit (2 ng/mL), good precision (CV < or = 4.2%) and speed (total analysis time 24 min) of the present method were sufficient for drug monitoring. This method was successfully applied to a bioequivalence test of two commercial tofisopam tablets.
authors.affiliation: Medicinal Resources Research Center, College of Pharmacy, Wonkwang University, Shinyongdong, Iksan 570-749, Korea.
authors.forename: Soo Kyoung
authors.initials: SK
authors.lastname: Baek
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Jeong Soo
authors.initials: JS
authors.lastname: Kim
authors.forename: Eun Jeon
authors.initials: EJ
authors.lastname: Park
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Hee-Yong
authors.initials: HY
authors.lastname: Lee
authors.forename: Hye Suk
authors.initials: HS
authors.lastname: Lee
mesh_terms: D000328:Adult; D014151:Anti-Anxiety Agents / Q000097:blood* / Q000493:pharmacokinetics; D001569:Benzodiazepines; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D008297:Male; D015203:Reproducibility of Results
chemical_list: D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; C012582:tofisopam