The mei3 region of the Schizosaccharomyces pombe genome.
Expression of the mei3 gene is sufficient to induce meiosis in the fission yeast Schizosaccharomyces pombe. The mei3 gene is located 0.64 Mb from the telomere of the left arm of Sz. pombe chromosome II. We have sequenced and analysed 107 kb of DNA from the mei3 genomic region. The sequence includes 14 known genes (bag1-B, csh3, dps1, gpt1, mei3, mfm3, pac1, prp31, rpl38-1, rpn3, rti1, spa1, spm1 and ubc4) and 26 other open reading frames (ORFs) longer than 100 codons: a density of one protein-coding gene per 2.7 kb. Twenty-one of the 40 ORFs (53%) have introns. In addition there is one lone Tf1 transposon long terminal repeat (LTR), tRNA(Trp) and tRNA(Ser) genes and a 5S rRNA gene. 14 of the novel ORFs show sequence similarities which suggest functions of their products, including a coatomer alpha-subunit, a catechol O-methyltransferase, protein kinase, asparagine synthetase, zinc metalloprotease, acetyltransferase, phosphatidylinositol 4-kinase, inositol polyphosphate phosphatase, GTPase-activating protein, permease, pre-mRNA splicing factor, 20S proteasome component and a thioredoxin-like protein. One predicted protein has similarity to the human Cockayne syndrome protein CSA and one with human GTPase XPA binding protein XAB1. Three ORFs are likely to code for proteins because they have sequence similarity with hypothetical proteins, three encode predicted coiled-coil proteins and four are sequence orphans.
Aves
Stephen J
SJ
School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK. S.J.Aves@exeter.ac.uk
Hunt
Cherryl
C
Xiang
Zheng
Z
Lyne
Michael H
MH
Wood
Valerie
V
Rajandream
Marie-Adèle
MA
Skelton
Jason
J
Churcher
Carol M
CM
Warren
Timothy
T
Harris
David
D
Gwilliam
Rhian
R
Barrell
Barclay G
BG
D003360:Cosmids; D005656:Fungal Proteins / Q000235:genetics*; D016681:Genome, Fungal; D008540:Meiosis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017421:Sequence Analysis
D005656:Fungal Proteins; C116305:MEI3 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins