title: Temporal changes in the distribution of thoracic duct lymphoblasts to synovium and other tissues of rats with adjuvant-induced arthritis.
abstract: The distribution of lymphoblasts(lymphocytes in cell cycle) obtained from the central lymph of donor rats and transferred adoptively to syngeneic recipients has been shown previously to be influenced by the presence of arthritis in either donor or recipient rats. The intent of the present study was to examine patterns of distribution of lymphoblasts in the early period after transfer, when extravasation of donor lymphoblasts was expected to occur. Thoracic duct lymphoblasts labelled in vitro with [125I]-iododeoxyuridine were detected in recipient rats by external radiometry and autoradiography. Irrespective of donor status, fewer donor lymphoblasts accumulated in the feet of normal recipients when compared to arthritic recipients at 15 min, 2 h and 24 h after cell transfer.When recipients of similar disease status were compared, the percentages of injected lymphoblasts from normal and arthritic donors recovered in the feet were similar at 15 min and 2 h after transfer. The proportions of lymphoblasts recovered in the feetat 24 h after injection declined in normal recipients and arthritic recipients of cells from normal donor rats. Importantly,this decline did not occur when both the donor and the recipient were arthritic. In the hindpaws, donor lymphoblasts were located predominantly in the bone marrow, except in transfers between arthriticrats, when at 24 h they were predominantly in the synovium. At 15 min, lymphoblasts were detected within the lumen of vessels within synovium, whereas by 2 h extravasation of these cells was evident. In conclusion, lymphoblasts accumulate more readily in hindfeet that are inflamed. In the early hours after injection, lymphoblasts from normal and arthritic donors are recruited equally, but these early levels are only maintained for 24 hin the combination of arthritic donor and arthritic recipient. Adramatic change in the proportion of lymphoblasts located in synoviumat this later time suggests that a dynamic process of relocation,retention and/or local cell division maintains the numbers of arthritic donor cells in the latter combination.
authors.affiliation: The Arthritis Research Laboratory of the Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, University of Adelaide, Adelaide, South Australia, Australia. lcleland@mail.rah.sa.gov.au
authors.forename: Leslie G
authors.initials: LG
authors.lastname: Cleland
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Wing
authors.forename: Llewellyn D J
authors.initials: LD
authors.lastname: Spargo
authors.forename: Graham
authors.initials: G
authors.lastname: Mayrhofer
mesh_terms: D000276:Adjuvants, Immunologic; D019264:Adoptive Transfer; D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D001345:Autoradiography; D002465:Cell Movement; D005260:Female; D007065:Idoxuridine / Q000032:analysis; D007700:Kinetics; D008213:Lymphocyte Activation; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013583:Synovial Membrane / Q000276:immunology* / Q000473:pathology; D013897:Thoracic Duct / Q000166:cytology / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D007065:Idoxuridine