A disposable acetylcholinesterase-based electrode biosensor to detect anatoxin-a(s) in water.
Anatoxin-a(s) is a hazardous toxin released by cyanobacteria during bacterial blooms. A simple and fast method to detect this hazardous compound using a biosensor based on the electrochemical detection of the activity of acetylcholinesterase was developed. Among several acetylcholinesterases, electric eel enzyme was found to be the most sensitive to anatoxin-a(s) and was thus used to build disposable amperometric sensors. The system displayed a detection limit of 1 microg/L anatoxin-a(s). No unspecific effect was noticed with real water samples but spiked toxin was accurately detected. Oxime reactivation was used to discriminate between the toxin and potential insecticides present in the sample.
Villatte
F
F
Institute for Technical Biochemistry, Stuttgart University, Germany.
Schulze
H
H
Schmid
R D
RD
Bachmann
T T
TT
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D000087522:Cyanobacteria Toxins; D004209:Disposable Equipment; D004566:Electrodes; D004593:Electrophorus; D004800:Enzymes, Immobilized / Q000378:metabolism; D008387:Marine Toxins / Q000032:analysis*; D052998:Microcystins; D014326:Tropanes
D001427:Bacterial Toxins; D002800:Cholinesterase Inhibitors; D000087522:Cyanobacteria Toxins; D004800:Enzymes, Immobilized; D008387:Marine Toxins; D052998:Microcystins; D014326:Tropanes; C509783:anatoxin a; D000110:Acetylcholinesterase