Use of multiplex PCR and PCR restriction enzyme analysis for detection and exploration of the variability in the free-living amoeba Naegleria in the environment.
A multiplex PCR was developed to simultaneously detect Naegleria fowleri and other Naegleria species in the environment. Multiplex PCR was also capable of identifying N. fowleri isolates with internal transcribed spacers of different sizes. In addition, restriction fragment length polymorphism analysis of the PCR product distinguished the main thermophilic Naegleria species from the sampling sites.
Pélandakis
Michel
M
Laboratoire de Biologie Cellulaire, EA 1655, Faculté de Pharmacie, 69373 Lyon Cedex 08, France. pelandak@rockefeller.univ-lyon1.fr
Pernin
Pierre
P
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D009253:Naegleria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity; D014867:Water / Q000469:parasitology*
D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D014867:Water