[Protective effects of schisanhenol against oxygen free radical induced injury of rat cerebral mitochondria and synaptosomes].
The cerebral tissue injury induced by ischemia and reperfusion or trauma has been considered to be due to over production of oxygen free radicals (OFRs). The aim of this study was to evaluate the effects of schisanhenol (Sal) on Fe(2+)-cysteine (Cys) induced injury of rat cerebral mitochondria and synaptosomes in vitro. Incubation of cerebral mitochondria or synaptosomes with Fe(2+)-Cys at 37 degrees C resulted in an increase of malondialdehyde (MDA) formation and decrease of ATPase activity. Sal(10(-4) mol.L-1) completely inhibited Fe(2+)-Cys induced increase of MDA formation of mitochondria and synaptosomes as well as the loss of ATPase activity of mitochondria. The swelling of mitochondria and reduction of membrane fluidity of mitochondria and synaptosomes induced by Fe(2+)-Cys were also prevented by Sal. Sal(10(-5) mol.L-1) was shown to significantly inhibit the decrease of synaptosomal GSH content induced by H2O2. The electron micrographs also showed that Sal markedly reduced the pathological damage of mitochondria and synaptosomes induced by Fe(2+)-Cys. The results suggest that Sal has protective action against Fe(2+)-Cys induced injury of rat cerebral mitochondria and synaptosomes.
Li
L
L
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050.
Liu
G
G
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure
D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D018696:Neuroprotective Agents; D011083:Polycyclic Compounds; D008315:Malondialdehyde; C045309:schisanhenol; D000251:Adenosine Triphosphatases; D005978:Glutathione