Reactivity of carboxyl groups in modified proteins.
The reactivities of carboxyl groups to carbodiimides in non-reduced, S-carboxymethyl, and S-cyanoethyl proteins have been compared. When disulphide bonds are split, a decrease in reactivity was found, relatively minor in the S-carboxymethyl derivative and almost complete loss of reactivities in the S-cyanoethyl derivative.
Frater
R
R
Division of Protein Chemistry, CSIRO, Parkville (Melbourne), 3052, Victoria, Australia