title: Homology modeling of falcipain-2: validation, de novo ligand design and synthesis of novel inhibitors.
abstract: Increasing resistance of malaria parasites, in particular Plasmodium falciparum, demands a serious search for novel targets. Cysteine protease in P. falciparum, encoded by a previously unidentified gene falcipain 2, provides one such target to design chemotherapeutic agents for treatment of malaria. In fact, a few cysteine protease inhibitors have been shown to inhibit growth of cultured malarial parasites. In absence of a crystal structure for this enzyme, homology modeling proved to be a reasonable alternative to study binding requirements of the enzyme. A homology model for falcipain 2 was developed and validated by docking of known vinyl sulfone inhibitors. Further, based on the observations of these studies, novel isoquinoline inhibitors were designed and synthesized, which exhibited in vitro enzyme inhibition at micromolar concentrations.
authors.affiliation: Department of Medicinal Chemistry, National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Sabnis
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Rosenthal
authors.forename: Prashant
authors.initials: P
authors.lastname: Desai
authors.forename: Mitchell A
authors.initials: MA
authors.lastname: Avery
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D015853:Cysteine Proteinase Inhibitors / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008024:Ligands; D008956:Models, Chemical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum; D011485:Protein Binding; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D015853:Cysteine Proteinase Inhibitors; D008024:Ligands; D003546:Cysteine Endopeptidases; C416224:falcipain 2