Aminoadipate reductase gene: a new fungal-specific gene for comparative evolutionary analyses.
BACKGROUND
In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6-semialdehyde. However, other organisms have no homologue to the aminoadipate reductase gene and this pathway appears to be restricted to fungi. In this study, we designed degenerate primers for polymerase chain reaction (PCR) amplification of a large fragment of the aminoadipate reductase gene for divergent fungi.


RESULTS
Using these primers, we amplified DNA fragments from the archiascomycetous yeast Saitoella complicata and the black-koji mold Aspergillus awamori. Based on an alignment of the deduced amino acid sequences, we constructed phylogenetic trees. These trees are consistent with current ascomycete systematics and demonstrate the potential utility of the aminoadipete reductase gene for phylogenetic analyses of fungi.


CONCLUSIONS
We believe that the comparison of aminoadipate reductase among species will be useful for molecular ecological and evolutionary studies of fungi, because this enzyme-encoding gene is a fungal-specific gene and generally appears to be single copy.
An
Kwang-Deuk
KD
Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. aa17122@mail.ecc.u-tokyo.ac.jp
Nishida
Hiromi
H
Miura
Yoshiharu
Y
Yokota
Akira
A
D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D000818:Animals; D019143:Evolution, Molecular; D005658:Fungi / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics*; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D008969:Molecular Sequence Data; D010802:Phylogeny; D013045:Species Specificity
D000445:Aldehyde Oxidoreductases; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase