title: Novel cryptosporidium genotypes in sporadic cryptosporidiosis cases: first report of human infections with a cervine genotype.
abstract: In this study, we genotyped parasites from the fecal specimens of sporadic cryptosporidiosis cases in British Columbia from 1995 to 1999. Genotyping was conducted by polymerase chain amplification of the internal transcribed spacer region, a hypervariable region in the 18S rRNA gene and the Cryptosporidium oocyst wall protein gene. Subsequent analysis was by restriction fragment length polymorphism and DNA sequencing. We identified two new Cryptosporidium genotypes in humans. One of these genotypes has been found recently in deer in New York state. The other genotype has not been identified in humans or animals. These results have important implications for drinking water quality strategies, especially for communities that obtain drinking water supplies from surface sources located in forested regions with deer populations.
authors.affiliation: University of British Columbia, Vancouver, BC, Canada. cong@interchange.ubc.ca
authors.forename: Corinne S L
authors.initials: CS
authors.lastname: Ong
authors.forename: Diane L
authors.initials: DL
authors.lastname: Eisler
authors.forename: Alireza
authors.initials: A
authors.lastname: Alikhani
authors.forename: Vicki W K
authors.initials: VW
authors.lastname: Fung
authors.forename: Joan
authors.initials: J
authors.lastname: Tomblin
authors.forename: William R
authors.initials: WR
authors.lastname: Bowie
authors.forename: Judith L
authors.initials: JL
authors.lastname: Isaac-Renton
mesh_terms: D000818:Animals; D001955:British Columbia; D003457:Cryptosporidiosis / Q000235:genetics*; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification; D003670:Deer; D005243:Feces / Q000469:parasitology; D016678:Genome; D005838:Genotype; D006801:Humans; D009518:New York; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D012337:RNA, Ribosomal, 18S