Effects of gatifloxacin on phagocytosis, intracellular killing and oxidant radical production by human polymorphonuclear neutrophils.
The ingestion and killing of bacteria by phagocytic cells is an important step in the sequence of interactions between invading microorganisms and host defense systems and may be affected by antibiotics. We investigated the effects of gatifloxacin on the phagocytosis, killing and oxidative bursts of human polymorphonuclear neutrophils (PMNs). The percentage phagocytosis and the phagocytosis index were unaffected by exposure of Escherichia coli strains to sub-MICs of gatifloxacin to a 1/64 dilution. However a significant increase in percentage intraphagocytic killing and the killing index occurred in one E. coli strain at 1/32 MIC and in two strains at 1/16 MIC. The incubation of PMNs with sub-MICs and supra-MICs of gatifloxacin (to 32 MIC) did not affect the oxidative bursts.
Braga
P C
PC
Center for Respiratory Pharmacology, Department of Pharmacology, School of Medicine, University of Milan, Via Vanvitelli 32, 20129 Milan, Italy. bragapc@mailserver.unimi.it
Dal Sasso
M
M
Bovio
C
C
Zavaroni
E
E
Fonti
E
E
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology; D024841:Fluoroquinolones; D000077734:Gatifloxacin; D006801:Humans; D008826:Microbial Sensitivity Tests; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology*; D010084:Oxidation-Reduction / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects*
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D000077734:Gatifloxacin