[Activities of the liver microsome reductases in adult and aged rats during stress].
The influence of 0.01% sodium dodecyl sulphate, 1.5 and 6.0 M urea and 0.03 M hydrogen peroxide to the NAD(P)H: 2,6 dichlorphenolindophenol reductase activity in livers of adult and old Wistar rats during immobilizing stress was interested. Obtained results indicate that the NADPH--dependent reductase is more resistant to modulating effect of sodium dodecyl sulphate, hydrogen peroxide and urea than NADH-dependent enzyme. The significant decrease of NADH: 2.6 dichlorphenolindophenol reductase sensitivity to the action of all studied modulators occurs in old rats. The similar changes appears in the adult rats liver during stress. The old rats immobilization is accompanied by a decrease of this enzyme activity and the reduction of the influence of all studied modulators to NADH: 2.6 dichlorphenolindophenol reductase as compared with adult ones. These changes in the activity and properties of microsomal NADH: 2,6 dichlorphenolindophenol reductase promote more pronounced decrease of the substrate hydroxylation in the liver of old rats during stress compared to adult ones.
Rud'ko
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NP
State Medical University, Mayakowsky av., 26, Zaporozhye, 69035, Ukraine.
Davydov
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VV
D000375:Aging / Q000378:metabolism*; D000818:Animals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007103:Immobilization; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011808:Quinone Reductases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013315:Stress, Psychological / Q000201:enzymology*; D014508:Urea / Q000494:pharmacology
D012967:Sodium Dodecyl Sulfate; D014508:Urea; D006861:Hydrogen Peroxide; D011808:Quinone Reductases; C033633:dichlorophenolindophenol reductase
Izuchenie osobennosteĭ reguliatsii aktivnosti reduktaz mikrosom pecheni vzroslykh i starykh krys pri stresse.