title: Biocompatibility evaluation of ePTFE membrane modified with PEG in atmospheric pressure glow discharge.
abstract: ePTFE membranes were modified by poly(ethylene glycol) having a molecular weight of 600 (PEG-600) in atmospheric pressure glow discharge (APG) plasma treatment. ePTFE membranes were immersed in 1%, 3%, or 5% (w/v) PEG-600 in dehydrated ethanol. PEG-600-penetrated ePTFE membranes were dried in a vacuum to immediately remove ethanol, then treated with APG at 20 kHz and 60-70 W for 15 min and thoroughly washed with ethanol and water. PEG-600-modified ePTFE membranes were analyzed using contact angle measurement, Fourier transform infrared attenuated total reflectance (FTIR-ATR), and scanning electron microscopy (SEM). ePTFE membrane contact angles were reduced after PEG-600 plasma treatment. FTIR-ATR spectra showed an absorption band due to a PEG hydroxyl group (-OH). SEM showed that ePTFE fiber surfaces were uniformly immobilized with PEG-600 and retained their porous structure. A general biological evaluation of the PEG-modified ePTFE membranes showed no cytotoxicity on CHO-K1 cell lines and no hemolytic action. Albumin adsorption on the PEG-modified ePTFE membranes increased with increasing PEG-600 deposited on ePTFE membranes. Fibrinogen adsorption decreased with increasing PEG-600 deposited on ePTFE membranes. gamma-Globulin adsorption did not change before or after PEG plasma modification. 1% and 3% PEG-600 plasma-treated ePTFE only slightly increased platelet adhesion, but adhering platelets evidenced no pseudopod formation. 5% PEG-600-modified ePTFE showed relatively large numbers of platelet adhesion. We concluded that 3% PEG-600-modified ePTFE membrane had the best physical properties and biological compatibility, indicating 3% PEG-600-modified ePTFE membranes exhibit the potential for blood filter application.
authors.affiliation: 3-D Tissue Engineering Group, National Institute for Advanced Interdisciplinary Research, Agency of Industrial Science and Technology, 1-1-4 Higashi, Tsukuba, Ibaraki 305-8562, Japan.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Chunren
authors.initials: C
authors.lastname: Wang
authors.forename: Yohannan
authors.initials: Y
authors.lastname: Babukutty
authors.forename: Toshie
authors.initials: T
authors.lastname: Ohyama
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Kogoma
authors.forename: Makoto
authors.initials: M
authors.lastname: Kodama
mesh_terms: D000327:Adsorption; D000818:Animals; D001274:Atmospheric Pressure; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000166:cytology; D002448:Cell Adhesion; D002470:Cell Survival; D006461:Hemolysis; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D012709:Serum Albumin; D011092:Polyethylene Glycols; D011138:Polytetrafluoroethylene