Cyclophilin a plays distinct roles in human immunodeficiency virus type 1 entry and postentry events, as revealed by spinoculation.
Cyclophilin A (CypA) is necessary for effective human immunodeficiency virus type 1 (HIV-1) replication. However, the functions of CypA and the precise steps at which CypA acts in the HIV-1 life cycle remain to be determined. By using a methodology that bypasses the need for attachment factors-spinoculation-we present evidence that CypA participates in both entry and postentry events.
Saphire
Andrew C S
AC
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Bobardt
Michael D
MD
Gallay
Philippe A
PA
D002498:Centrifugation / Q000379:methods; D021984:Cyclophilin A / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011133:Polysaccharide-Lyases / Q000378:metabolism
D011133:Polysaccharide-Lyases; C021349:heparitinsulfate lyase; D021984:Cyclophilin A