Clinical, genetic and microbiological findings in a Brazilian family with aggressive periodontitis.
OBJECTIVE
Aggressive periodontitis comprises a group of rapidly progressive forms of periodontitis. Besides bacteria, a high level of subject susceptibility must be involved in the expression of disease. In the present study, we report the clinical, microbiological and genetic profile of a 14-individual family with aggressive periodontitis.


METHODS
PCR was utilized to detect pathogenic bacteria of affected sites. DNA was obtained from epithelial cells through a mouthwash with 3% glucose and scrapping of the oral mucosa. RFLP-PCR was used to analyze cytokine genetic polymorphisms.


RESULTS
Localized aggressive periodontitis was diagnosed for an 18-year-old systemically healthy non-smoking proband, with siblings displaying aggressive periodontitis. Bacteroides forsythus and Treponema denticola were the most frequent pathogens. The proband presented Actinobacillus actinomycetemcomitans and detectable levels of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola. Allele 2 of IL-1alpha (-889) polymorphism was found in all individuals as well as allele 1 of the IL-1beta (+3953) gene. Alleles 1 and 2 (50 % each) of IL-1beta (-511), allele 1 of TNF-alpha (-308) and allele 2 (in homo or heterozygosity) of IL-RN (intron 2) gene were present.


CONCLUSIONS
The results show that the present microbiological and genetic parameters were not relevant for the prediction of periodontitis susceptibility in this family.
Trevilatto
Paula C
PC
Department of Morphology, Dental School of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Tramontina
Vinícius A
VA
Machado
Maria A N
MA
Gonçalves
Reginaldo B
RB
Sallum
Antonio W
AW
Line
Sergio R P
SR
D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D000483:Alleles; D001439:Bacteroides / Q000302:isolation & purification; D001938:Brazil; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D010518:Periodontitis / Q000235:genetics* / Q000382:microbiology* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D012307:Risk Factors; D014208:Treponema / Q000302:isolation & purification
D007375:Interleukin-1; D017472:Receptors, Interleukin-1