abstract: The terminal cis-glycol-group of phenylalanine specific tRNA (tRNA(Phe), I) from yeast was oxidized to the dialdehyde by NaIO(4) (tRNA(Phe)(oxi), II) and subsequently reduced to the diol by NaBH(4) (tRNA(Phe)(oxi-red), III). In charging experiments it could be shown that tRNA(Phe)(oxi-red) is still completely specific for phenylalanine. The Michaelis constant of tRNA(Phe)(oxi-red) remains unchanged when compared with tRNA(Phe), while the maximun velocity of charging upsilon(max) of tRNA(Phe)(oxi-red) is decreased to about one half.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie, 3400, Göttingen, Germany
authors.forename: F
authors.initials: F
authors.lastname: Cramer
authors.forename: F v.d.
authors.initials: F
authors.lastname: Haar
authors.forename: E
authors.initials: E
authors.lastname: Schlimme
vernacular_title: Uber das aminoacylierungsverhalten chemisch modifizierter phenylalaninspezifischer transfer-ribonucleinsäure aus hefe: (1) Glykolspaltung und reduktion zum diol an der 3'-terminalen ribose.