Characterisation of monoclonal antibodies against haemagglutinin associated with Clostridium botulinum type C neurotoxin.
Of 11 monoclonal antibodies (MAbs) prepared against the non-toxic component of type C Clostridium botulinum 16S toxin to clarify the function of the non-toxic component, seven recognised HA1, three recognised HA3b and one recognised HA2. Results of epitope mapping indicated that three of the seven anti-HA1 MAbs recognised the region between amino acid residues 121 and 140 and four recognised the three-dimensional structure of HA1. Three anti-HA3b MAbs recognised different regions between (approximately) amino acids 405-430, 180-270 and 275-297. The ability of these MAbs to interfere with binding of 16S toxin or non-toxic component, HA1 or HA3b to erythrocytes and to intestine tissue sections of guinea-pig was observed. MAbs against HA3b and HA2 did not inhibit 16S toxin binding to either erythrocytes or epithelial cells, whereas some MAbs against HA1 did inhibit binding. The seven anti-HA1 MAbs can be classified into four groups based on their binding inhibition activities. The anti-HA1 MAbs that inhibited the binding of 16S toxin to the epithelial cells also neutralised or reduced the oral toxicity in mice, indicating that HA may play an important role in the absorption of the 16S toxin from the small intestine.
Mahmut
Nazira
N
Inoue
Kaoru
K
Fujinaga
Yukako
Y
Hughes
Lynn
L
Arimitsu
Hideyuki
H
Sakaguchi
Yoshihiko
Y
Ohtsuka
Aiji
A
Departments of Bacteriology and *Human Morphology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
Murakami
Takuro
T
Departments of Bacteriology and *Human Morphology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
Yokota
Kenji
K
Oguma
Keiji
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification / Q000276:immunology*; D001427:Bacterial Toxins / Q000276:immunology; D015153:Blotting, Western; D001905:Botulinum Toxins / Q000737:chemistry / Q000276:immunology*; D010450:Endopeptidases / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology; D007422:Intestines / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D011817:Rabbits
C116382:16S toxin, Clostridium botulinum; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D006388:Hemagglutinins; D007106:Immune Sera; D007074:Immunoglobulin G; D010450:Endopeptidases; D001905:Botulinum Toxins; C096324:botulinum toxin type C