[The effects of paraoxonase-1 and oxidized low density lipoprotein on nephropathy in type-2 diabetes mellitus].
OBJECTIVE
To explore the effect of paraoxonase-1 (PON-1) and oxidized low density lipoprotein (ox-LDL) in diabetic nephropathy (DN).


METHODS
Serum PON-1 activity was measured by using phenyl acetate as a substrate and the concentration of plasma ox-LDL was determined by ELISA in 91 cases of type 2 diabetes mellitus (DM). Simultaneously, serum NO, VWF and GMP 140 were also determined. The patients of DM were divided into three groups according to urine albumine excretion rate (UAER) (DM I: UAER < 30 mg/24 h; DM II: UAER 30 approximately 300 mg/24 h; DM III: UAER > 300 mg/24 h) and matched with normal controls (NC).


RESULTS
In the three groups of DM, PON-1 were markedly decreased and ox-LDL were obviously increased (P < 0.01). Both of their levels were different in the three groups of DM (P < 0.05). PON-1 were negatively correlated with ox-LDL (r = -0.467, P < 0.01). There were negative correlation between PON-1 and urine albumin (r = -0.697, P < 0.01), but ox-LDL was positively correlated with urine albumin (r = 0.747, P < 0.01). Serum nitrites (NO) level in DM I was significantly higher than that in NC, but the NO level in DM II and DM III was lower than that in DM I and NC. GMP 140 and VWF levels in all the 3 DM groups were higher than that in NC. PON-1 was positively correlated with NO (r(s) = 0.326, P < 0.01), and negatively correlated with VWF (r(s) = -0.365, P < 0.01). ox-LDL was negatively correlated with NO (r(s) = -0.196, P < 0.05), but positively correlated with VWF and GMP 140 (r(s) = 0.294, P < 0.05; r(s) = 0.669, P < 0.01 respectively). Logistic regression showed that ox-LDL was a dangerous factor for DN.


CONCLUSIONS
PON-1, ox-LDL, NO, endothelial and platelet function are connected with DN; they affect one another and influence the occurrence and development of DN together.
Wang
Hongman
H
Department of Endocrinology, The First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016, China.
Deng
Huacong
H
Liu
Wanli
W
D000328:Adult; D043303:Aryldialkylphosphatase; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D003928:Diabetic Nephropathies / Q000097:blood*; D004950:Esterases / Q000097:blood*; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D019007:P-Selectin / Q000097:blood; D014841:von Willebrand Factor / Q000032:analysis
D008077:Lipoproteins, LDL; D009566:Nitrates; D019007:P-Selectin; C099252:oxidized low density lipoprotein; D014841:von Willebrand Factor; D009569:Nitric Oxide; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human