[Preparation of rhBMP-2/BCB reconstituted bone xenograft and assay of its osteoinductivity].
OBJECTIVE
To investigate a new grafting material of bone xenograft with strong bone inductive and conductive capacity.


METHODS
Based on successful clinical application of the reconstituted bone xenograft (RBX), a new xenograft was made by combining recombinant human bone morphogenetic protein-2 (rhBMP-2) with antigen-free bovine cancellous bone (BCB). Sixty male BALB/C mice aged 4 weeks were divided into study group of 30 and control group of 30 randomly. rhBMP-2/BCB was implanted in the left thigh muscle pouch in the study group and BCB in the control group. The mice were sacrificed at 7 d, 14 d and 21 d after implantation. Inductivity of rhBMP-2/BCB was detected by histological observation and biochemical determination of the samples.


RESULTS
Histological examination showed that rhBMP-2/BCB induced chondrogenesis on the 7th day, with woven bone formed on the 14th day, and lamellar bone and marrow on the 21st day, while BCB failed to induce chondrogenesis or osteogenesis on the 7th, 14th and 21st days. The alkaline phosphatase activities and calcium content in study group were higher than those in control group with significant difference (P < 0.01).


CONCLUSIONS
rhBMP-2/BCB is an ideal grafting material with strong bone inductive and conductive capacity without evoking immune reaction.
Yuan
Zhi
Z
Institute of Orthopedics, Xijing Hospital of Fourth Military Medical University, Xi'an, Shaanxi, P. R. China 710032.
Ma
Ping
P
Hu
Yun-Yu
YY
Zhao
Guang-yue
GY
Lu
Rong
R
Sun
Liang
L
Li
Dan
D
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D018786:Bone Substitutes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects*; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014183:Transplantation, Heterologous
C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta