title: DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia.
abstract: OBJECTIVE
  To confirm the genetic relation between Giardia lamblia (G. lamblia) isolates from different geographic regions of China and other countries.
  
  
  METHODS
  Genomic DNA were extracted from the trophozoites or cysts of Giardia lamblia. The triose phosphate isomerase (tim) gene was amplified using polymerase chain reaction (PCR) technique. PCR products were digested with endonuclease and sequenced. The data of sequencing were analyzed with the DNAstar software and compared with that of the isolates acquired from GenBank.
  
  
  RESULTS
  Of nine isolates of Giardia lamblia from China (C1, C2, CH2 and CH3), Cambodia (CAM), Australia (A1 and A2) and America (BP and CDC), respectively, 3 (A1, A2 and CAM) fit into Group 1 (WB), 2 (CH2 and CH3)) into Group 2, and 4 (C1, C2, BP and CDC) into Group 3 (GS). The results confirmed the genetic relatedness of G. lamblia isolates from all over the world.
  
  
  CONCLUSIONS
  Genotyping isolates of G. Lamblia provides important information for establishing the phylogenetic relationship or for the epidemiological evaluation of the spreading of this organism.
authors.affiliation: Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China. Lusiqi@cpums.edu.cn
authors.forename: Siqi
authors.initials: S
authors.lastname: Lu
authors.forename: Jianfan
authors.initials: J
authors.lastname: Wen
authors.forename: Jihong
authors.initials: J
authors.lastname: Li
authors.forename: Fengyun
authors.initials: F
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry*; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014305:Triose-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D014305:Triose-Phosphate Isomerase