title: (13)C[(13)C] 2D NMR: a novel strategy for the study of paramagnetic proteins with slow electronic relaxation rates.
abstract: Oxidized human [2Fe-2S] ferredoxin has a notably slow electronic relaxation rate, which precludes the observation of signals from nuclei near the iron-sulfur cluster by conventional 2D or 3D methods that utilize proton detection. We have demonstrated the utility of (13)C[(13)C]CT-COSY in identifying connectivity information from fast relaxing carbon nuclei near the paramagnetic center, including those from residues that ligate the metal center.
authors.affiliation: National Magnetic Resonance Facility at Madison, Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Machonkin
authors.forename: William M
authors.initials: WM
authors.lastname: Westler
authors.forename: John L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D002247:Carbon Isotopes; D005288:Ferredoxins / Q000737:chemistry*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010084:Oxidation-Reduction
chemical_list: D002247:Carbon Isotopes; D005288:Ferredoxins