title: Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase.
abstract: Triphosphate of a new fluorescent labeled thymidine analogue was incorporated as a substrate for PCR using KOD Dash DNA polymerase forming the corresponding fluorescent labeled DNA which is useful for a DNA probe.
authors.affiliation: Department of Applied Chemistry, Gunma University, Kiryu, 376-8515, Gunma, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Obayashi
authors.forename: Mohammed M
authors.initials: MM
authors.lastname: Masud
authors.forename: Akiko N
authors.initials: AN
authors.lastname: Ozaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ozaki
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sawai
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D015342:DNA Probes; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013936:Thymidine / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine