Should gaps be included in chromosomal aberration analysis? Evidence based on the comet assay.
This study evaluated DNA damage in human lymphocytes due to occupational exposure to low levels of ionizing radiation using two assays: the comet assay and chromosomal aberration (CA) analysis including and excluding gaps. The results obtained reveal a higher correlation between both methods when chromatid and chromosome gaps were included in the correlation analysis (r=0.78 versus r=0.50). This increased correlation support the hypothesis that the gaps constitute a type of chromosome aberration, and suggest that these events should be scored in this type of analysis.
Paz-y-Miño
César
C
Laboratorio de Genética Molecular y Citogenética Humana, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, P.O. Box 17-1-2184, Quito, Ecuador. cpazymino@puceuio.puce.edu.ec
Dávalos
María Verónica
MV
Sánchez
María Eugenia
ME
Arévalo
Melissa
M
Leone
Paola E
PE
D000328:Adult; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004784:Environmental Monitoring; D005260:Female; D006282:Health Personnel; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D011832:Radiation Injuries / Q000175:diagnosis* / Q000235:genetics; D011834:Radiation Monitoring / Q000379:methods; D011839:Radiation, Ionizing; D011871:Radiology