title: Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization.
abstract: The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 degrees C was sufficient to decrease the carbon content in the serum sample to 5%-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time of 10-2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181.
authors.affiliation: Laboratory of Plasma Sources for Chemical Analysis, Faculty of Science, Masaryk University Brno, Czech Republic.
authors.forename: Jirí
authors.initials: J
authors.lastname: Machát
authors.forename: Viktor
authors.initials: V
authors.lastname: Kanický
authors.forename: Vítezslav
authors.initials: V
authors.lastname: Otruba
mesh_terms: D006801:Humans; D012015:Reference Standards; D012643:Selenium / Q000097:blood*; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D012643:Selenium