Correlation of survivin mRNA detection with histologic diagnosis in normal endometrium and endometrial carcinoma.
BACKGROUND
The objective of this study was to determine if survivin mRNA expression is a marker of endometrioid adenocarcinoma and if survivin mRNA levels correlate with tumor grade and stage.


METHODS
Twenty-six samples of endometrioid adenocarcinoma and 18 cases of benign endometrium were obtained at surgery. RNA was extracted from tissues and was used for quantitative real-time RT-PCR, targeting a 91-bp sequence of survivin mRNA and the levels were standardized to the levels of ribosomal RNA. Statistical analysis of the correlation between histologic diagnosis and the corrected survivin mRNA levels was performed by the Fisher Exact test and the Kruskal-Wallis test.


RESULTS
Survivin mRNA was detected in all specimens. Survivin mRNA levels were increased in proliferative endometrium (P = 0.0509) and was increased in correlation with ascending grade in endometrioid adenocarcinoma (P = 0.01).


CONCLUSIONS
Survivin mRNA is not a specific marker of endometrial cancer, but may reflect an important mechanism in tumor progression of the endometrial mucosa.
Lehner
Rainer
R
Department of Pathology, University of Colorado Health Sciences Center, East Ninth Avenue, Denver, Colarado, USA.
Enomoto
Takayuki
T
McGregor
James A
JA
Shroyer
Laurie
L
Haugen
Bryan R
BR
Pugazhenthi
Umarani
U
Shroyer
Kenneth R
KR
D000230:Adenocarcinoma / Q000473:pathology*; D000704:Analysis of Variance; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D046508:Culture Techniques; D016889:Endometrial Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins; D016015:Logistic Models; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009367:Neoplasm Staging; D011336:Probability; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D000077022:Survivin
C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D002868:Chromosomal Proteins, Non-Histone; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000077022:Survivin