The major light-harvesting pigment protein of Acaryochloris marina.
The major light-harvesting protein complex containing chlorophyll (Chl) d was isolated from Acaryochloris marina thylakoid membranes. Isolation was achieved by detergent solubilisation followed by separation on 6-40% sucrose gradients using ultracentrifugation. The best Chl d yield (70%) used 0.3% dodecyl maltoside, 0.15% octyl glucoside, 0.05% zwittergent 3-14 with the detergent:total Chl d ratio around 10:1 (w/w). Characterisation of the light-harvesting pigment protein complex (lhc) involved non-denaturing electrophoresis, SDS-PAGE, absorbance and fluorescence spectroscopy. The main polypeptide in the lhc was shown to be ca. 34 kDa and to contain Chl d and Chl a, indicating that the Acaryochloris lhc is similar to that of prochlorophytes. The Chl a level varied with the culture conditions, which is consistent with previous findings.
Chen
Min
M
School of Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
Quinnell
Rosanne G
RG
Larkum
Anthony W D
AW
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D000458:Cyanobacteria; D003902:Detergents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification; D048928:Prochlorophytes; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D020524:Thylakoids / Q000737:chemistry
D001426:Bacterial Proteins; D003902:Detergents; D045322:Photosynthetic Reaction Center Complex Proteins