Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry.
The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI-MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristic. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97-F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments.
Prasain
Jeevan Kumar
JK
Antibiotics Laboratory, RIKEN, 2-1 Hirosawa Wako, Saitama 351-0198, Japan.
Ueki
Masashi
M
Stefanowicz
Piotr
P
Osada
Hiroyuki
H
D002851:Chromatography, High Pressure Liquid; D003572:Cytochalasins / Q000032:analysis*; D005285:Fermentation; D005658:Fungi / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
D003572:Cytochalasins