title: Cytogenetic evaluation of human oocytes that failed to complete meiotic maturation in vitro.
abstract: OBJECTIVE
  To determine the cause of infertility in a couple whose oocytes failed to mature in two consecutive fertility treatments.
  
  
  METHODS
  Case report.
  
  
  METHODS
  University-based IVF program.
  
  
  METHODS
  A 32-year-old woman with unexplained infertility.
  
  
  METHODS
  Cytogenetic evaluation of oocytes that failed to reach meiotic metaphase II stage of maturation.
  
  
  METHODS
  Observation of oocyte maturity and chromosome composition after fixing and staining with Orcein stain.
  
  
  RESULTS
  Cytogenetic analysis revealed that the oocytes had successfully resumed meiosis. Germinal vesicle breakdown was also indicated, and chromosomes were at metaphase II stage of development. However, meiotic reduction of those chromosomes failed.
  
  
  CONCLUSIONS
  Infertility in this couple seems to be attributed to the failure of the chromosomes to complete the reduction phase of metaphase II of meiosis.
authors.affiliation: Hamilton Health Sciences, Ontario, Hamilton, Canada. nealm@hhsc.ca
authors.forename: Michael S
authors.initials: MS
authors.lastname: Neal
authors.forename: Lisa
authors.initials: L
authors.lastname: Cowan
authors.forename: Jackie Pierre
authors.initials: JP
authors.lastname: Louis
authors.forename: Ed
authors.initials: E
authors.lastname: Hughes
authors.forename: W Allan
authors.initials: WA
authors.lastname: King
authors.forename: Pari K
authors.initials: PK
authors.lastname: Basrur
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002875:Chromosomes / Q000648:ultrastructure; D020732:Cytogenetic Analysis; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000235:genetics*; D008297:Male; D008540:Meiosis; D008677:Metaphase; D009865:Oocytes / Q000648:ultrastructure*