[Rapid quantitation of follistatin by surface plasmon resonance (SPR) immunoassay].
A simple, rapid, and accurate assay using surface plasmon resonance (SPR) apparatus with anti-follistatin antibody (SPR immunoassay) has been developed for the quantitation of recombinant follistatin. This assay can be performed with a direct injection of conditioned medium; results were obtained within 10 min. The quantitation component of this assay was precise and accurate with a limit of quantitation of 62.5 ng/ml in Ham's F12 medium containing 2% fetal bovine serum. These results demonstrate that SPR immunoassay is a powerful technique for several researches, especially for screening of gene transfectant and monitoring of protein production.
Hyuga
M
M
mhyuga@nihs.go.jp
Kawasaki
N
N
Hyuga
S
S
Ohta
M
M
Itoh
S
S
Hayakawa
T
T
D028341:Activins / Q000032:analysis* / Q000096:biosynthesis; D000818:Animals; D001709:Biotechnology; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D038681:Follistatin; D007118:Immunoassay / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis; D020349:Surface Plasmon Resonance; D013678:Technology, Pharmaceutical
D003470:Culture Media; D038681:Follistatin; D011994:Recombinant Proteins; D028341:Activins