The expression of the Helicobacter pylori genes ureA and nap is higher in vivo than in vitro as measured by quantitative competitive reverse transcriptase-PCR.
The expression of the virulence-associated genes ureA, encoding the urease subunit A, and nap, encoding the neutrophil activating protein, in Helicobacter pylori grown both in the stomach of C57/Bl6 mice and in Brucella broth was quantified by quantitative competitive reverse transcriptase-PCR using a homologous RNA standard (competitor) and an external standard (16S rRNA). The results showed that the ureA and nap transcripts were increased up to 15 and 80 times, respectively, in vivo compared to in vitro. The transcription of ureA and nap also differed in that ureA showed highest expression early in infection in mice whereas nap transcription was variable throughout the 18-week infection period.
Blom
Kristina
K
Department of Medical Microbiology and Immunology, Göteborg University, P.O. Box 435, SE-40530, Göteborg, Sweden.
Svennerholm
Ann Mari
AM
Bölin
Ingrid
I
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D016480:Helicobacter pylori / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014510:Urease / Q000235:genetics*
D001426:Bacterial Proteins; C092412:neutrophil-activating protein A, Helicobacter pylori; D014510:Urease