Making ends meet in genetic analysis using padlock probes.
Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single-nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tag-arrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable.
Nilsson
Mats
M
Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden.
Banér
Johan
J
Mendel-Hartvig
Maritha
M
Dahl
Fredrik
F
Antson
Dan-Oscar
DO
Gullberg
Mats
M
Landegren
Ulf
U
D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011088:DNA Ligases / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
D015342:DNA Probes; D012313:RNA; D004247:DNA; D011088:DNA Ligases