[Culture and cryopreservation of human mandibular condylar cartilage cells].
OBJECTIVE
To establish an effective way in cryopreserving mandibular condylar cartilage cells.


METHODS
Human mandibular condylar cartilage (MCC) cells were harvested and amplified by microcarrier culture technique. MCC cells were preserve in liquid nitrogen. The biological properties of cryopreserved MCC cells were investigated.


RESULTS
Cells from liquid nitrogen preservation kept their phenotypic, proliferative an metabolic characteristics of MCC cells in vivo.


CONCLUSIONS
Cryopreservation of MCC cells can supply adequate qualified cells for repairing the defects of mandibular condyle cartilage and temporomandibular joint disk.
Jiao
Y
Y
Center for Temporomandibular Joint Disorders and Orofacial Pain, Peking University School of Stomatology, Beijing 100081, China.
Ma
X
X
Yu
S
S
D002356:Cartilage / Q000166:cytology*; D018929:Cell Culture Techniques; D015925:Cryopreservation; D006801:Humans; D008335:Mandibular Condyle / Q000166:cytology*