Regional variations in the distributions of small intestinal intraepithelial lymphocytes (IELs) in BALB/c +/+, nu/+, and nu/nu mice.
Regional variations in intraepithelial lymphocytes (IELs) in the small intestine were examined in BALB/c +/+, nu/+, and nu/nu mice. The small intestine was obtained from 11- to 12-week-old mice and divided equally into three (proximal, middle, and distal) parts. The IELs were isolated from each part of the intestine, and the total numbers of IELs in nu/+ and nu/nu mice were about a fifth of those in +/+ mice. Regional variations in the distribution of the IEL alphabeta, but not the gammadelta T-cell subset were found by use of flow cytometry in +/+ and nu/+ mice. On the other hand, such differences were not found in nu/nu mice, suggesting that thymus-independent development of T cells is not different among regions. Different local expansion of thymus-dependent alphabeta T cells may cause the regional variations seen in the distribution of alphabeta T cell IELs in +/+ and nu/+ mice.
Suzuki
H
H
Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo, Japan.
Jeong
K I
KI
Doi
K
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D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D019070:Cell Lineage; D005838:Genotype; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D016130:Immunophenotyping; D007421:Intestine, Small / Q000166:cytology*; D018655:Lymphocyte Count; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000033:anatomy & histology* / Q000235:genetics; D008819:Mice, Nude / Q000033:anatomy & histology* / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012376:Rodent Diseases / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D016176:T-Lymphocyte Subsets; D013950:Thymus Gland / Q000002:abnormalities / Q000473:pathology
D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta