[Effects of cadmium on 3H-leucine incorporation and cell growth of cultured myocardiocytes].
The effects of cadmium on 3H-leucine incorporation and cell growth of cultured cardiac myocytes were investigated by measuring the incorporation quantity of radioisotope labelled amino-acid and the cell volume on cultured myocardiocytes in neonatal Wistar rats. The results showed that 3H-leucine incorporation was decreased significantly in 1 x 10(-4) mmol/L CdCl2 for 24 h, and was gradually reduced with prolongation. The cell volume was decreased after exposed to Cd for 48 h. The volume was from (2053 +/- 189) microns 3 to (1847 +/- 176) microns 3. There was no significant difference in cell number between the treated group and the control group. These data indicated that cadmium can inhibit the synthesis of protein and the growth on cultured myocardiocytes.
Wang
Q
Q
Department of Physiology, Kaifeng Medical College, Kaifeng 475001, China.
Yao
S
S
D000818:Animals; D000831:Animals, Newborn; D002104:Cadmium / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007930:Leucine / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium
D002104:Cadmium; D014316:Tritium; D007930:Leucine