title: Differentiation of embryonic stem cells is induced by GATA factors.
abstract: Extraembryonic endoderm (ExE) is differentiated from the inner cell mass of the late blastocyst-stage embryo to form visceral and parietal endoderm, both of which have an important role in early embryogenesis. The essential roles of Gata-6 and Gata-4 on differentiation of visceral endoderm have been identified by analyses of knockout mice. Here we report that forced expression of either Gata-6 or Gata-4 in embryonic stem (ES) cells is sufficient to induce the proper differentiation program towards ExE. We believe that this is the first report of a physiological differentiation event induced by the ectopic expression of a transcription factor in ES cells.
authors.affiliation: Stem Cell Regulation Research, Area of Molecular Therapeutics, Course of Advanced Medicine, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Fujikura
authors.forename: Eiji
authors.initials: E
authors.lastname: Yamato
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Yonemura
authors.forename: Kiminori
authors.initials: K
authors.lastname: Hosoda
authors.forename: Shinji
authors.initials: S
authors.lastname: Masui
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Miyazaki Ji
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004707:Endoderm / Q000378:metabolism; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D050993:GATA6 Transcription Factor; C494759:GATA6 protein, human; C494760:Gata6 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors