[Experimental study on suppressive effect of ginkgo extract on adhesion of vascular endothelial cell to monocyte induced by minimally modified low density lipoprotein].
OBJECTIVE
To observe the effect of Ginkgo extract on the interaction between minimally modified low density lipoprotein (MM-LDL) induced human umbilical vein endothelial cell (HUVEC) and U937 monocyte-like cell line.


METHODS
The adhesive percentage between HUVEC and U937 by counting and the expression of VCAM-1, ICAM-1, P-selectin by ELISA.


RESULTS
Treatment of HUVEC with MM-LDL (75 micrograms/ml) for 4 hrs could significantly increase the adhesion of U937 to HUVEC (P < 0.01) and Ginkgo extract could suppress the adhesion in dose-dependent manner. The surface expression of VCAM-1, ICAM-1, P-selectin was not induced by MM-LDL, while recombination tumor necrosis factor-alpha 5.0 ng/ml, as a positive control, could do.


CONCLUSIONS
Ginkgo extract afforded protection against HUVEC damage induced by MM-LDL and the adhesion of monocytes to endothelial cells induced by MM-LDL is not mediated by VCAM-1, ICAM-1 and P-selectin.
Liu
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C
Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300020.
Qian
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G
Liu
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H
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology*; D020441:Ginkgo biloba / Q000737:chemistry*; D006801:Humans; D007231:Infant, Newborn; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology*; D018515:Plant Leaves / Q000737:chemistry; D020298:U937 Cells / Q000166:cytology; D014471:Umbilical Veins / Q000166:cytology
D004365:Drugs, Chinese Herbal; D008077:Lipoproteins, LDL