Novel osteoblast-adhesive peptides for dental/orthopedic biomaterials.
Next generation dental/orthopedic biomaterials must be designed to enhance and support osteoblast adhesion. The osteoblasts use different ways to adhere, that is, integrin- and proteoglycan-mediated mechanisms. The present study reports on the synthesis and osteoblast-adhesive properties of peptides carrying RGD motifs and of sequences mapped on human vitronectin. Our data suggest that osteoblast adhesion on polystyrene plates modified with a linear peptide, in which the GRGDSP sequence is repeated four times, was significantly higher when compared to the adhesion obtained using branched peptides, interestingly containing the same motif. Osteoblast adhesion assays on acellular bone matrix using this active peptide gave very promising results. We also demonstrated that a novel peptide, carrying the X-B-B-B-X-B-B-X motif (where B is a basic amino acid and X is a nonbasic residue), promotes proteoglycan-mediated osteoblast adhesion more efficiently with respect to the KRSR sequence that was recently proposed as heparan-sulfate binding peptide.
Dettin
Monica
M
Department of Chemical Process Engineering, University of Padova, Padova, Italy.
Conconi
Maria Teresa
MT
Gambaretto
Roberta
R
Pasquato
Antonella
A
Folin
Marcella
M
Di Bello
Carlo
C
Parnigotto
Pier Paolo
PP
D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D003764:Dental Materials; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
D001672:Biocompatible Materials; D003764:Dental Materials; D010455:Peptides