title: Purification and characterization of 3,3-dihydroxyazetidine from culture medium of Bacillus mesentericus and B. subtilis.
abstract: A method is described for the purification of 3,3-dihydroxyazetidine (DHA), which accelerates the growth of Bifidobacterium spp., from the culture medium of Bacillus mesentericus (BM). Purification involved adsorption to an ion-exchange resin; it required less time and gave a higher yield than a previously reported method. Monitoring the inhibition of E. coli NIHJ JC-2, we searched for other strains that produced 3,3-dihydroxyazetidine. We found that not only B. mesentericus TO-A but also B. subtilis IFO13719 produced the compound of interest.
authors.affiliation: Department of Microbiology, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Ozone
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Inoue
authors.forename: Akiko
authors.initials: A
authors.lastname: Shiraishi
authors.forename: Hajime
authors.initials: H
authors.lastname: Hamashima
authors.forename: Kazuo
authors.initials: K
authors.lastname: Masuda
authors.forename: Kenji
authors.initials: K
authors.lastname: Shiojima
authors.forename: Masanori
authors.initials: M
authors.lastname: Sasatsu
mesh_terms: D001384:Azetidines / Q000302:isolation & purification*; D001407:Bacillus / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D019936:Probiotics / Q000494:pharmacology
chemical_list: D001384:Azetidines; D003470:Culture Media