title: Impact of different gases and pneumoperitoneum pressures on tumor growth during laparoscopy in a rat model.
abstract: BACKGROUND
  The influence on intraperitoneal tumor growth of the choice of gas and pneumoperitoneum pressure during laparoscopy is still unknown. This study compared tumor growth after laparoscopy with different gases and pneumoperitoneum pressures in an immunodeficient model.
  
  
  METHODS
  In an initial experiment, 60 nude rats were randomly allocated to undergo laparoscopy at different pneumoperitoneum pressures (gasless, 4 mmHg, or 8 mm Hg.) In a second experiment, 23 nude rats were randomly allocated to undergo laparoscopy with different gases (carbon dioxide or helium). Surgery was carried out 7 days after intraperitoneal injection of IGR-OV1 cells. The rats were killed 7 days after surgery. Tumor growth was assessed by the weight of the omental metastasis. For statistical analysis, we used analysis of variance (ANOVA).
  
  
  RESULTS
  Mean omental weight was similar for all groups, regardless of the pneumoperitoneum pressure (p = 0.86) or the type of gas (p = 0.80).
  
  
  CONCLUSIONS
  Physical parameters of gas have a limited impact on tumor growth.
authors.affiliation: Department of Obstetrics and Gynecology, La Conception University Hospital, Marseille, 147 Boulevard Baille, 13385 Cedex 05, France. aubert.agostini@wanadoo.fr
authors.forename: A
authors.initials: A
authors.lastname: Agostini
authors.forename: F
authors.initials: F
authors.lastname: Robin
authors.forename: J P
authors.initials: JP
authors.lastname: Jais
authors.forename: M
authors.initials: M
authors.lastname: Aggerbeck
authors.forename: F
authors.initials: F
authors.lastname: Vildé
authors.forename: B
authors.initials: B
authors.lastname: Blanc
authors.forename: F
authors.initials: F
authors.lastname: Lécuru
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D005260:Female; D006371:Helium; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D009852:Omentum; D010051:Ovarian Neoplasms / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000556:secondary*; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous
chemical_list: D002245:Carbon Dioxide; D006371:Helium