title: Biosynthesis of hibarimicins. I. 13C-labeling experiments.
abstract: Biosynthesis of hibarimicin was studied based on the feeding experiments with 13C labeled acetates. All carbons in the aglycon, except for the methoxy carbons, were derived from acetate. The carbon framework of the aglycon was proved to be constructed by dimerization of an intermediate which was biosynthesized via the decarboxylation and skeltal rearrangement starting from an undecaketide. The rearrangement was confirmed by detecting the long range (three-bond) coupling between two carbons in the difference spectra of selective 13C decoupled INADEQUATE of hibarimicin B labeled with sodium [1,2-13C2] acetate.
authors.affiliation: Department of Applied Biological Chemistry, Tamagawa University, Machida, Tokyo, Japan. hhori@agr.tamagawa.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hori
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kajiura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Igarashi
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Furumai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Higashi
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Ishiyama
authors.forename: Masakazu
authors.initials: M
authors.lastname: Uramoto
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Uehara
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Oki
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents