title: Mechanism of regulation of the bifunctional histidine kinase NtrB in Escherichia coli.
abstract: NtrB is the bifunctional histidine kinase for nitrogen regulation. Dependent on the availability of nitrogen, it either autophosphorylates and serves as the phosphodonor for its cognate response regulator, NtrC, or, it promotes the rapid dephosphorylation of NtrC-P. The activity of NtrB depends on the interaction of two subdomains within its transmitter domain, the H-domain and the kinase domain. Both phosphotransfer activity and phosphatase activity reside in the H-domain. When separately expressed, this domain acts as a phosphatase. Interaction with the kinase domain results in the inhibition of the phosphatase activity and the phosphorylation of the conserved histidine of the H-domain.
authors.affiliation: Department of Biology, University of Konstanz, Germany. weiss@chclu.chemie.uni-konstanz.de
authors.forename: Verena
authors.initials: V
authors.lastname: Weiss
authors.forename: Günter
authors.initials: G
authors.lastname: Kramer
authors.forename: Thomas
authors.initials: T
authors.lastname: Dünnebier
authors.forename: Annette
authors.initials: A
authors.lastname: Flotho
mesh_terms: D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009584:Nitrogen / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D011494:Protein Kinases; C055699:protein kinase-phosphatase NTRB; D010749:Phosphoprotein Phosphatases; D009584:Nitrogen