title: SufC hydrolyzes ATP and interacts with SufB from Thermotoga maritima.
abstract: Genetic experiments in bacteria have shown the suf operon is involved in iron homeostasis and the oxidative stress response. The sufB and sufC genes that always occur together in bacteria are also found in plants, and even the malaria parasite, associated with the plastid organelle. Although the suf operon is believed to encode an iron-dependent ABC-transporter there is no direct evidence. By immunolocalization we show here that SufB and SufC are associated with the membrane of Escherichia coli. We also present kinetic studies with a recombinant version of SufC from Thermotoga maritima that shows it is an ATPase and that it interacts with SufB in vitro.
authors.affiliation: National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
authors.forename: K
authors.initials: K
authors.lastname: Rangachari
authors.forename: C T
authors.initials: CT
authors.lastname: Davis
authors.forename: J F
authors.initials: JF
authors.lastname: Eccleston
authors.forename: E M A
authors.initials: EM
authors.lastname: Hirst
authors.forename: J W
authors.initials: JW
authors.lastname: Saldanha
authors.forename: M
authors.initials: M
authors.lastname: Strath
authors.forename: R J M  Iain
authors.initials: RJ
authors.lastname: Wilson
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000502:physiology; D016133:Polymerase Chain Reaction; D011485:Protein Binding / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017421:Sequence Analysis; D013455:Sulfur / Q000378:metabolism; D020124:Thermotoga maritima / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D013455:Sulfur; D000255:Adenosine Triphosphate; D007501:Iron; D000251:Adenosine Triphosphatases