Fasciclin domain proteins are present in nostoc symbionts of lichens.
Differences in the soluble protein fraction between the freshly isolated cyanobiont of lichen Peltigera membranacea, the corresponding free-living strain, and Nostoc punctiforme were analyzed. One protein, which was among the most prominent proteins of the freshly isolated cyanobiont, was expressed at a lower level in the corresponding free-living strain and was not detected at all on the two-dimensional gels of N. punctiforme. This protein was partially sequenced, and the corresponding open reading frame (ORF) in the N. punctiforme genome was identified. This ORF contains a fasciclin domain typical of a class of surface-associated proteins involved in cell adhesion. Similar fasciclin motif-containing genes have previously been shown to be symbiotically induced in other symbiotic systems.
Paulsrud
Per
P
Department of Physiological Botany, Evolutionary Biology Centre, Uppsala University, SE-752 36 Uppsala, Sweden.
Lindblad
Peter
P
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000458:Cyanobacteria / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008011:Lichens; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D013559:Symbiosis
D001426:Bacterial Proteins