title: [Pituitary adenylate cyclase activating polypeptide ameliorates the damage and inhibits the increase of intracellular calcium concentration in cultured hippocampal neurons induced by glutamate].
abstract: Hippocampal neurons cultured from 7 to 9 d in vitro were used to observe the effect of glutamate. Treatment of glutamate for 24 h greatly decreased neuronal survival and pretreatment with pituitary adenylate cyclase activating polypeptide (PACAP) significantly attenuated hippocampal neuron death induced by glutamate. Moreover, glutamate dose-dependently increased the intracellular calcium concentration in cultured hippocampal neurons, while PACAP inhibited the increase of intracellular calcium concentration induced by glutamate. PACAP 6-38, a specific PACAP type I receptor antagonist, completely inhibited the amelioration of glutamate induced death and the decrease of intracellular calcium concentration induced by PACAP in cultured hippocampal neurons. The data suggest that PACAP has a neuroprotective effect on the hippocampal neuronal damage induced by glutamate, which is related to an inhibition of glutamate-induced increase of intracellular calcium concentration and mediated by PACAP type I receptor.
authors.affiliation: Department of Neurobiology, Second Military Medical University, Shanghai 200433, China. dongyk@online.sh.cn
authors.forename: Y
authors.initials: Y
authors.lastname: Dong
authors.forename: T S
authors.initials: TS
authors.lastname: Tang
authors.forename: C L
authors.initials: CL
authors.lastname: Lu
authors.forename: C
authors.initials: C
authors.lastname: He
authors.forename: J B
authors.initials: JB
authors.lastname: Dong
authors.forename: X Y
authors.initials: XY
authors.lastname: Huang
authors.forename: F Z
authors.initials: FZ
authors.lastname: Sun
authors.forename: X
authors.initials: X
authors.lastname: Bao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003864:Depression, Chemical; D018698:Glutamic Acid; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D009479:Neuropeptides / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D018696:Neuroprotective Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D018698:Glutamic Acid; D002118:Calcium