[Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos].
Extracts of spinal dorsal horn and ventral horn of chicken embryos hatched for 9 days were separated by Sephadex G-75 gel filtration, and different chromatographic fractions of DI and DII, VI and VII were obtained. The neurite-outgrowth promoting effects of the various fractions were assayed by the dorsal root ganglion (DRG) culture in vitro. It was shown that DII, but not DI, fraction of the dorsal horn extract exhibited a significant neurite-outgrowth promoting effect on DRG. By means of SDS-PAGE, the molecular weight of DII fraction ranged from 61 to 15 kD. VI and VII fraction of ventral horn showed no obvious effect on neurit-outgrowth of DRG in vitro.
Liu
Y B
YB
The Institute of Basic Medical Sciences, Academy of Military Medicine, Beijing 100850. liuyb@nic.bmi.ac.cn
Xue
Q S
QS
Xiao
Y P
YP
Wang
X R
XR
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology; D009414:Nerve Growth Factors / Q000032:analysis / Q000302:isolation & purification*; D013116:Spinal Cord / Q000737:chemistry*
D009414:Nerve Growth Factors