Microscope-based techniques to study cell adhesion and migration.
Modern light microscopy has evolved to provide a variety of quantitative imaging techniques and also the capability to perturb structure-function relationships in living cells. The advances have been especially useful in the study of cell adhesion and migration. This review will focus on how such microscopy-based techniques can be useful in situ to study the molecular interactions and dynamics, to locally perturb actin-based structures and to measure the traction forces exerted by motile cells.
Roy
Partha
P
Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7090 USA.
Rajfur
Zenon
Z
Pomorski
Pawel
P
Jacobson
Ken
K
D000199:Actins / Q000648:ultrastructure; D001692:Biological Transport; D002448:Cell Adhesion; D002465:Cell Movement; D003584:Cytological Techniques; D004735:Energy Transfer; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods*; D008954:Models, Biological; D013050:Spectrometry, Fluorescence / Q000379:methods
D000199:Actins