Fibrin sheath enhances central venous catheter infection.
OBJECTIVE
To determine whether fibrin-coated central venous catheters have a higher infection rate, and spawn more septic emboli, than uncoated catheters after exposure to bacteremia.


METHODS
Animal study comparing catheter infection and blood cultures of fibrin-coated and uncoated catheters exposed to bacteremia.


METHODS
Animal laboratory.


METHODS
Adult male Sprague-Dawley rats.


METHODS
A total of 210 rats had catheters placed with the proximal end buried subcutaneously. Rats were divided into three groups: tail vein bacterial injection on day 0 (no fibrin group) or on day 10 (fibrin group), or no injection/saline injection (control, n = 40). Bacterial injections were 1 x 108 colony forming units of either Staphylococcus epidermidis (n = 100) or Enterobacter cloacae (n = 60). Animals were killed 3 days after injection. Blood cultures were obtained via cardiac puncture, and catheters were removed via the chest. Half of the catheter was rolled onto agar and the other half was placed in trypticase soy broth. Plates and broth were incubated at 37 degrees C for 48 hrs. The presence of >15 colonies on roll plates, or growth in broth, was accepted as a positive sign of infection. Microscopy was performed on day 20-10 catheters. Thirty animals without catheters had bacterial injections and underwent blood culture 3 days after injection.


RESULTS
Catheter infection with S. epidermidis occurred in 32% of roll plates and 80% of broth from the fibrin group vs. 4% and 20% from the no fibrin group (p <.01 for each). Catheter infection with E. cloacae occurred in 50% of roll plates and 80% of broth from the fibrin group vs. 0% and 12% from the no fibrin group (p <.01 for each). Positive blood cultures occurred in 47 of 68 animals from the fibrin group vs. 8 of 68 from the no fibrin group (p <.01). Microscopy showed a fibrin sheath on 20 of 20 catheters. Without catheters, 30 of 30 blood cultures were negative.


CONCLUSIONS
The fibrin sheath significantly enhanced catheter-related infection and persistent bacteremia.
Mehall
John R
JR
Department of Pediatric Surgery, Arkansas Children's Hospital, the University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Saltzman
Daniel A
DA
Jackson
Richard J
RJ
Smith
Samuel D
SD
D000818:Animals; D001422:Bacterial Adhesion; D002404:Catheterization; D002405:Catheterization, Central Venous / Q000295:instrumentation*; D016972:Enterobacter cloacae / Q000302:isolation & purification; D004866:Equipment Contamination; D004867:Equipment Design; D005337:Fibrin; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000209:etiology*; D013212:Staphylococcus epidermidis / Q000302:isolation & purification
D005337:Fibrin