Topical corticosteroids of limited potency promote adenovirus replication in the Ad5/NZW rabbit ocular model.
OBJECTIVE
To determine the effect of topical therapy with several corticosteroids with limited potency on viral clearance in the adenovirus type 5 (Ad5) rabbit ocular model.


METHODS
Sixty rabbits were inoculated in both eyes with Ad5. On the first day, the rabbits were equally divided into four topical treatment groups: 0.12% prednisolone acetate (PA), 0.1% fluorometholone (FM), 1% rimexolone (RMX), and control. Treatment was administered four times daily, in both eyes, for 3 days. All eyes were cultured for virus on days 0, 1, 3, 4, 5, 7, 9, 11, 14, 16, 18, and 21.


RESULTS
Compared with the control group, treatment with PA, FM, and RMX significantly increased the number of Ad5 positive eye cultures from days 7-21. Fluorometholone and RMX prolonged the duration of Ad5 shedding, and FM increased the mean combined Ad5 titer from days 1-5 and 7-21.


CONCLUSIONS
Treatment of an experimental ocular adenovirus infection with PA, FM, and RMX for 3 days significantly enhanced adenovirus replication compared with the control group. Short-term treatment of EKC with several commercially available topical corticosteroids with limited potency may offer symptomatic relief, but may also delay viral clearance and promote office and community epidemics.
Romanowski
Eric G
EG
The Charles T. Campbell Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Yates
Kathleen A
KA
Gordon
Y Jerold
YJ
D000257:Adenoviridae Infections / Q000188:drug therapy* / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification / Q000502:physiology*; D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003315:Cornea / Q000821:virology*; D005260:Female; D005469:Fluorometholone / Q000008:administration & dosage / Q000627:therapeutic use; D005938:Glucocorticoids; D007637:Keratoconjunctivitis / Q000188:drug therapy* / Q000821:virology; D023421:Models, Animal; D011239:Prednisolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D011245:Pregnadienes / Q000008:administration & dosage / Q000627:therapeutic use; D011817:Rabbits; D014779:Virus Replication / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D011245:Pregnadienes; C009935:prednisolone acetate; D011239:Prednisolone; C013769:rimexolone; D005469:Fluorometholone