title: Association of the phosphatidylinositol signal pathway with prolonged myocardial ischemia.
abstract: OBJECTIVE
  To study the changes in activity of phosphatidylinositol 4 kinase (PI 4 kinase), phosphatidylinositol 4 phosphate 5 kinase (PIP 5 kinase) and protein kinase C (PKC) during myocardial ischemia and elucidate the relationship between phosphatidylinositol signal pathways and prolonged myocardial ischemia.
  
  
  METHODS
  In vivo an ischemic rat model was used. Activity of PI 4 kinase, PIP 5 kinase and PKC were measured at different times in postischemic heart cells using isotope analysis.
  
  
  RESULTS
  The activity of PI kinase, PIP kinase and PKC in the myocardium increased to peak at 1 hour postischemia, with activities 6.1, 3.0 and 4.0 fold over control levels, respectively. Their activities declined to normal levels with time.
  
  
  CONCLUSIONS
  The phosphatidylinositol signal pathway is involved in prolonged myocardial ischemia, but its mechanism needs further study.
authors.affiliation: Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China. Dingxiuyun@mailexcite.com
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ding
authors.forename: Jiachang
authors.initials: J
authors.lastname: Yue
authors.forename: Shiwen
authors.initials: S
authors.lastname: Wang
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Li
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism*; D000818:Animals; D008297:Male; D017202:Myocardial Ischemia / Q000201:enzymology*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D017853:Phosphotransferases (Alcohol Group Acceptor); D019870:1-Phosphatidylinositol 4-Kinase; C020077:1-phosphatidylinositol-4-phosphate 5-kinase; D011493:Protein Kinase C