Application of rep-PCR fingerprinting for genotyping of Escherichia coli strains in Wojnowskie Wschodnie and Wojnowskie Zachodnie lake.
The paper presents usefulness of application of the PCR-based fingerprinting method, which uses enterobacterial repetitive intergenic consensus primers ERIC (rep-PCR (ERIC)) in analysing and characterising Escherichia coli population in the water environment. 46 E. coli isolates of homogenous biochemical properties were analysed. The received results prove considerable genomic diversity among the analysed isolates. The used technique has turned to be a reproducible and rapid method with a considerable differentiation power. The introductory research has revealed that the technique may be successfully used in qualitative research, for intra-species differentiation of microorganisms occurring in water environment.
Baldy-Chudzik
K
K
Institute of Biotechnology and Environmental Protection, University of Zielona Góra, Poland.
Niedbach
J
J
Stosik
M
M
D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics; D005618:Fresh Water; D014644:Genetic Variation; D010802:Phylogeny; D011044:Poland; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014871:Water Microbiology
D004269:DNA, Bacterial