title: [The effects of butyrates and non-steroidal anti-inflammatory agents on HT-29 colonic carcinoma cells].
abstract: OBJECTIVE
  Adenocarcinoma cells HT-29 were used to investigate whether butyrates and non-steroidal anti-inflammatory agents (NSAIDs, aspirin and NS-398) would be effective adjuvants for inducing apoptosis and to probe its possible mechanisms.
  
  
  METHODS
  Prostaglandin E(2) (PGE(2)) isolated from HT-29 cell culture supernants was investigated with ELISA. Flow cytometry was used for estimating apoptosis. The morphology of apoptotic cells was investigated by means of electron microscopy.
  
  
  RESULTS
  Butyrates could stimulate the secretion of PGE(2). In contrast, NSAIDs inhibited it to a level below 30 ng/L. Both butyrates and NSAIDs could induce apoptosis. The effects showed the obvious tendency of time- and concentration-dependency (P < 0.05). Combination with two drugs could enhance the above effects.
  
  
  CONCLUSIONS
  Butyrates and NSAIDs could induce apoptosis individually. Combination with the two drugs could enhance the above effects by down-regulating cyclooxygenase-2 expression.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital, Western China University of Medical Sciences, Chengdu 610041, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Ouyang
authors.forename: Huatian
authors.initials: H
authors.lastname: Gan
mesh_terms: D000230:Adenocarcinoma; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis; D001241:Aspirin / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology*; D003110:Colonic Neoplasms; D015232:Dinoprostone / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D019073:HT29 Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009578:Nitrobenzenes / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002087:Butyrates; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D015232:Dinoprostone; D001241:Aspirin