[Cholecalciferol Reference Standard (Control 001) of National Institute of Health Sciences].
The raw material of cholecalciferol was examined for the preparation of "Cholecalciferol Reference Standard (Control 001)". Analytical data obtained were: melting point, 83.2 degrees C; UV and infrared spectra, the same as those of JP Cholecalciferol Reference Standard (Control 971), respectively; specific absorbance at 265 nm, E1ca1% = 478; optical rotation, [alpha]D20 = +108.6 degrees; thin-layer chromatography, no impurities were detected until 100 micrograms; high-performance liquid chromatography (HPLC), total amount of impurities estimated to be less than 0.05%. Based on the above results, the raw material was authorized as the Japanese Pharmacopoeia Cholecalciferol Reference Standard (Control 001).
Maekawa
K
K
Iwata
M
M
Koide
T
T
Saito
H
H
Tanimoto
T
T
Okada
S
S
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002762:Cholecalciferol / Q000032:analysis / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D006077:Government Agencies; D007564:Japan; D010602:Pharmacopoeias as Topic / Q000592:standards; D012015:Reference Standards
D002762:Cholecalciferol