title: Partial purification and determination of molecular weights of glyoxalases by filtration on dextran gel.
abstract: Glyoxalase I and glyoxalase II (EC. 4.4.1.5 and EC 3.1.2.6) were separated by gel filtration on Sephadex G-75 and G-100. This simple procedure permitted also the partial purification of glyoxalase II. The purification coefficient in a single run from supernatant from beef liver was about 1 : 30 compared with 1 : 15 after the fifth step of purification with classical methods.
authors.affiliation: Department of Biochemistry, Medical Academy, Zabrze, Poland
authors.forename: T
authors.initials: T
authors.lastname: Jerzykowski
authors.forename: W
authors.initials: W
authors.lastname: Matuszewski
authors.forename: R
authors.initials: R
authors.lastname: Winter