In vitro uptake of [1-14C]Octanoate in brain slices of rats: basic studies for assessing [1-11C]Octanoate as a PET tracer of glial functions.
To clarify the contribution of glial cells to octanoate uptake into the brain, we determined the effects of fluoroacetate, a selective inhibitor of glial metabolism, on in vitro brain uptake of [1-14C]octanoate, using rat brain slices. The [1-14C]octanoate uptake significantly decreased, depending on the concentration of fluoroacetate (p = 0.001). The [1-14C]octanoate uptakes at 5 mM (0.23 +/- 0.05% uptake/mg slice) and 25 mM fluoroacetate (0.12 +/- 0.01% uptake/mg slice) were significantly lower than that at control (0.29 +/- 0.02% uptake/mg slice, p < 0.05 and p < 0.001, respectively). The results demonstrate the contribution of glial cells to octanoate uptake into the brain. The potential of [1-11C]octanoate as a PET tracer for studying glial functions is suggested.
Kuge
Yuji
Y
Department of Tracer Kinetics, Graduate School of Medicine, Hokkaido University, Kita 15 Nishi 7, Kita-ku, 060-8638, Sapporo, Japan. kuge@med.hokudai.ac.jp
Hikosaka
Kenji
K
Seki
Koh-ichi
K
Ohkura
Kazue
K
Nishijima
Ken-ichi
K
Tsukamoto
Eriko
E
Tamaki
Nagara
N
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002210:Caprylates / Q000493:pharmacokinetics*; D005463:Fluoroacetates / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature; D014055:Tomography, Emission-Computed / Q000379:methods*
D000963:Antimetabolites; D002210:Caprylates; D005463:Fluoroacetates; D019275:Radiopharmaceuticals; C014815:fluoroacetic acid; C031492:octanoic acid