Inactivation of the regulatory gene algU or gacA can affect the ability of biocontrol Pseudomonas fluorescens CHA0 to persist as culturable cells in nonsterile soil.
Rifampin-resistant Pseudomonas fluorescens CHA0-Rif and mutants in which the regulatory gene algU (encoding sigma factor sigma(E)) or gacA (encoding a global regulator of secondary metabolism) was inactivated were compared for persistence in three nonsterile soils. Functional algU and (particularly) gacA were needed for CHA0-Rif to maintain cell culturability in soil.
Mascher
Fabio
F
Phytopathology Group, Institute of Plant Science, Swiss Federal Institute of Technology (ETH), CH-8092 Zürich, Switzerland.
Moënne-Loccoz
Yvan
Y
Schnider-Keel
Ursula
U
Keel
Christoph
C
Haas
Dieter
D
Défago
Geneviève
G
D001426:Bacterial Proteins / Q000235:genetics*; D015169:Colony Count, Microbial; D003470:Culture Media; D005809:Genes, Regulator / Q000235:genetics*; D009154:Mutation; D010572:Pest Control, Biological; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000254:growth & development*; D012808:Sigma Factor / Q000235:genetics*; D012988:Soil Microbiology
C079619:AlgU protein, Pseudomonas aeruginosa; D001426:Bacterial Proteins; D003470:Culture Media; C091100:GacA protein, Bacteria; D012808:Sigma Factor