Screening of Chinese and Mongolian herbal drugs for anti-human immunodeficiency virus type 1 (HIV-1) activity.
Water and methanol extracts of 30 Chinese and Mongolian medicinal plants were tested for their human immunodeficiency virus type-1 (HIV-1) inhibitory activity. Of the 60 extracts, 23 showed anti-HIV activity. Bioassay-guided fractionation of one of the most active extracts, the methanol extract of the root tuber of Stephania cepharantha, led to the isolation of two alkaloids, aromoline and FK-3000 as potent inhibitory substances. They completely inhibited the cytopathic effects of HIV-1 on MT-4 cells at 31.3 and 7.8 microg/mL, respectively.
Ma
Chao-mei
CM
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Nakamura
Norio
N
Miyashiro
Hirotsugu
H
Hattori
Masao
M
Komatsu
Katsuko
K
Kawahata
Takuya
T
Otake
Toru
T
D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology*; D019380:Anti-HIV Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002681:China; D004365:Drugs, Chinese Herbal; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000737:chemistry / Q000494:pharmacology*; D008986:Mongolia; D010936:Plant Extracts / Q000494:pharmacology; D018514:Plant Structures / Q000737:chemistry; D010946:Plants, Medicinal / Q000145:classification; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D019380:Anti-HIV Agents; D004365:Drugs, Chinese Herbal; C442856:FK3000; D007546:Isoquinolines; D010936:Plant Extracts; C034784:obamegine; C066341:aromoline