title: Somatic embryogenesis from Arabidopsis shoot apical meristem mutants.
abstract: Zygotic embryos of three Arabidopsis thaliana (L.) Heynh. mutants lacking an embryonic shoot apical meristem (SAM), shoot meristemless (stm), wuschel (wus) and zwille/pinhead (zll/pnh) were used as explants to establish embryogenic cell cultures. Somatic embryos of all three mutants showed the same mutant phenotypes as their zygotic equivalents. These results provide genetic evidence that the developmental program of somatic and zygotic embryos is indistinguishable. They also suggest that a functional SAM is not required for somatic embryogenic cell formation in Arabidopsis.
authors.affiliation: Laboratory of Molecular Biology, Department of Biomolecular Sciences, Wageningen University, Dreijenlaan 3, 6703 HA, The Netherlands.
authors.forename: Andreas P
authors.initials: AP
authors.lastname: Mordhorst
authors.forename: Marijke V
authors.initials: MV
authors.lastname: Hartog
authors.forename: Mazen K
authors.initials: MK
authors.lastname: El Tamer
authors.forename: Thomas
authors.initials: T
authors.lastname: Laux
authors.forename: Sacco C
authors.initials: SC
authors.lastname: de Vries
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics / Q000254:growth & development; D002455:Cell Division / Q000235:genetics; D046508:Culture Techniques; D018519:Meristem / Q000166:cytology / Q000254:growth & development*; D009154:Mutation; D010641:Phenotype; D018520:Plant Shoots / Q000166:cytology / Q000254:growth & development*