Establishment of an interaction model of human apolipoprotein H with lipid monolayer by capillary SDS gel electrophoresis.
Apolipoprotein H (ApoH) is a plasma glycoprotein isolated from human serum. It plays a key role in the interaction with lipids. For the first time, the concentration of ApoH adsorbed on the lipid monolayer has been determined, and was done so using capillary electrophoresis. Based on this determination, an interaction model of ApoH and lipid monolayer was constructed, and this interaction is one of nonspecific adsorption. A neutral coated capillary (50 cm x 100 microm i.d.) and a negative voltage of 15 kV were used to separate ApoH. The calibration curve of ApoH was built using a detection limit of 50 microg/mL(-1) (near to 1 microM), and the RSD of the relative migration time of ApoH was 1.4% (n = 7).
Ru
Qin-Hua
QH
School of Life Science and Engineering, Tsinghua University, Beijing, People's Republic of China.
Wang
Yi-Ming
YM
Luo
Guo-An
GA
Wang
Shao-Xiong
SX
Sui
Shen-Fang
SF
D000327:Adsorption; D019075:Electrophoresis, Capillary / Q000379:methods; D006023:Glycoproteins / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D053482:beta 2-Glycoprotein I
D006023:Glycoproteins; D008081:Liposomes; D053482:beta 2-Glycoprotein I; D012967:Sodium Dodecyl Sulfate