Effects of propofol and taurine on intracellular free amino acid profiles and immune function markers in neutrophils in vitro.
We have examined the effects of propofol, taurine, and the combination of propofol and taurine on amino acid profiles and the immune function markers superoxide anion (O2-), hydrogen peroxide (H2O2), and released myeloperoxidase (MPO) activity in neutrophils (PMN). Propofol led to significant changes in the dynamic PMN-free amino acid pool. Exogenous taurine significantly reduced PMN neutral amino acid and alpha-aminobutyrate (alpha-aba) as intracellular taurine increased. Incubation with propofol plus taurine resulted in lower intracellular taurine levels and elevated alpha-aba and neutral amino acid concentrations compared to propofol alone. Concerning PMN immune function markers, propofol significantly decreased O2- and H2O2 formation and released MPO. Taurine led to an increased release of MPO and concomitant significantly reduced O2- and H2O2 levels. When propofol and taurine were applied together they appeared to act additively with regard to superoxide and hydrogen peroxide formation. In the case of MPO, taurine neutralized propofol's effects, supporting the idea that MPO activity may be regulated by taurine. We believe therefore that taurine is important for strengthening PMN host defense capability, although the mechanisms are not yet clear. Moreover, taurine appears to act primarily by altering the PMN osmotic balance, while propofol seems to affect PMN amino acid metabolism and/or uptake and release.
Mühling
Jörg
J
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, University Medical Center, Faculty of Medicine, Giessen, Germany. joerg.muehling@chiru.med.uni-giessen.de
Campos
Marie E
ME
Sablotzki
Armin
A
Krüll
Matthias
M
Dehne
Marius G
MG
Gonther
Jens
J
Weiss
Stefan
S
Fuchs
Markus
M
Hempelmann
Gunter
G
D000328:Adult; D000596:Amino Acids / Q000097:blood*; D015415:Biomarkers; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D015742:Propofol / Q000494:pharmacology*; D013654:Taurine / Q000494:pharmacology*
D000596:Amino Acids; D015415:Biomarkers; D013654:Taurine; D015742:Propofol