Behavior, neurochemistry and histology after intranigral lipopolysaccharide injection.
Inflammation and neuronal degeneration of the substantia nigra (SN) occur in Parkinson's disease (PD). We studied the effects of intranigral lipopolysaccharide (LPS) injection on adult Sprague-Dawley rats. Locomotor activity measurement, neurotransmitter determination and perfusion fixation for immunohistochemistry were done on the 7th day. Bilateral LPS injection increased locomotor activity 2- to 3-fold. In the SN, dopamine (DA) and serotonin (5-HT) decreased but the ratios dihydroxyphenylacetic acid (DOPAC)/DA, homovanillic acid (HVA)/DA and 5-hydroxyindole-acetic acid (5-HIAA)/5-HT increased. In the striatum, DA, DOPAC, HVA, 3-methoxytyramine and epinephrine decreased but HVA/DA and 5-HIAA/5HT ratios increased. Unilateral LPS decreased dopamineric neurons ipsilaterally but increased contralaterally. This study provides the first evidence of behavioral hyperactivity, epinephrine suppression and neuronal plasticity in the LPS model of PD.
Hsieh
Peiyuan F
PF
Division of Neurology, 160 Chung-Kang Road Section 3, Taichung, Taiwan 40705, ROC.
Chia
Lie-Gan
LG
Ni
Dah-Ren
DR
Cheng
Lee-Ju
LJ
Ho
Yuh-Pin
YP
Tzeng
Shun-Fen
SF
Chang
Ming-Hong
MH
Hong
Jau-Shyong
JS
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001923:Brain Chemistry / Q000187:drug effects*; D004298:Dopamine / Q000502:physiology; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D008845:Microinjections; D009043:Motor Activity / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000166:cytology / Q000473:pathology / Q000502:physiology*
D008070:Lipopolysaccharides; D018377:Neurotransmitter Agents; D004298:Dopamine