title: Interleukin-8 induction and adhesion of the coccoid form of Helicobacter pylori.
abstract: To determine the pathological significance of the coccoid form of Helicobacter pylori, its adhesion to and induction of secretion of interleukin-8 (IL-8) by gastric epithelial (MKN45) cells were studied. By flow cytometry, the adhesion of the coccoid form to MKN45 cells was significantly lower than that of the helical form. The monoclonal antibody A20 recognising lipopolysaccharide of H. pylori inhibited the adhesion of the coccoid form to MKN45 cells as much as that of the helical form. There was significantly lower induction of IL-8 secretion by MKN45 cells exposed to the coccoid form (two of four strains) as compared with the helical form.
authors.forename: Takako
authors.initials: T
authors.lastname: Osaki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Taguchi
authors.affiliation: Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Fukada
authors.affiliation: Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
authors.forename: Hayato
authors.initials: H
authors.lastname: Kawakami
authors.affiliation: Division of Medical Microbiology, Department of Infectious Diseases, *Laboratory for Electron Microscopy and †2nd Department of Anatomy, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirano
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kamiya
mesh_terms: D000693:Anaerobiosis; D001422:Bacterial Adhesion / Q000276:immunology; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000276:immunology* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity* / Q000648:ultrastructure; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured
chemical_list: D016209:Interleukin-8