title: Novel osteoblast-adhesive peptides for dental/orthopedic biomaterials.
abstract: Next generation dental/orthopedic biomaterials must be designed to enhance and support osteoblast adhesion. The osteoblasts use different ways to adhere, that is, integrin- and proteoglycan-mediated mechanisms. The present study reports on the synthesis and osteoblast-adhesive properties of peptides carrying RGD motifs and of sequences mapped on human vitronectin. Our data suggest that osteoblast adhesion on polystyrene plates modified with a linear peptide, in which the GRGDSP sequence is repeated four times, was significantly higher when compared to the adhesion obtained using branched peptides, interestingly containing the same motif. Osteoblast adhesion assays on acellular bone matrix using this active peptide gave very promising results. We also demonstrated that a novel peptide, carrying the X-B-B-B-X-B-B-X motif (where B is a basic amino acid and X is a nonbasic residue), promotes proteoglycan-mediated osteoblast adhesion more efficiently with respect to the KRSR sequence that was recently proposed as heparan-sulfate binding peptide.
authors.affiliation: Department of Chemical Process Engineering, University of Padova, Padova, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Roberta
authors.initials: R
authors.lastname: Gambaretto
authors.forename: Antonella
authors.initials: A
authors.lastname: Pasquato
authors.forename: Marcella
authors.initials: M
authors.lastname: Folin
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Bello
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D003764:Dental Materials; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology* / Q000648:ultrastructure; D010455:Peptides / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001672:Biocompatible Materials; D003764:Dental Materials; D010455:Peptides