title: A dorsal root ganglia cell line derived from trisomy 16 fetal mice, a model for Down syndrome.
abstract: We have established two immortalized cell lines from dorsal root ganglia of normal (G4b) and trisomy 16 mice (GT1), a model for Down syndrome. By immunohistochemistry, both cell lines exhibit neuronal traits and lack glial markers. GTl cells exhibited greater [3H]choline uptake than G4b cells. K+ and nicotine-mediated acetylcholine release was greater in GT1 cells. Basal intracellular Ca2+ concentration ([Ca2+]i) was significantly lower in GTl cells. More GTl cells responded to neurotransmitters with a transient [Ca2+]i increase compared to G4b cells, but both cell types showed similar amplitudes of [Ca2+]i responses. The results show that both cell lines retain neuronal characteristics and respond to specific neurotransmitter stimuli. Altered GT1 cell responses could be related to neuronal pathophysiology in Down's syndrome.
authors.affiliation: Department of Pharmaceutical Sciences, Texas Tech University HSC, Amarillo, Texas, USA.
authors.forename: David D
authors.initials: DD
authors.lastname: Allen
authors.forename: Ana María
authors.initials: AM
authors.lastname: Cárdenas
authors.forename: Christian
authors.initials: C
authors.lastname: Arriagada
authors.forename: Lori B
authors.initials: LB
authors.lastname: Bennett
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: García
authors.forename: Raúl
authors.initials: R
authors.lastname: Caviedes
authors.forename: Stanley I
authors.initials: SI
authors.lastname: Rapoport
authors.forename: Pablo
authors.initials: P
authors.lastname: Caviedes
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005333:Fetus; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011247:Pregnancy; D014314:Trisomy / Q000235:genetics*
chemical_list: D002118:Calcium