A novel method of labeling and characterizing migrating neurons in the developing central nervous system.
Neuronal migration, a discrete event in the developing nervous system, is currently being intensively investigated using a variety of anatomical and molecular approaches. Using 4-chloromethyl benzoyl amino tetramethyl rhodamine (CMTMR) coated particles, we describe here a novel and efficient method of tracer labeling to investigate cell migration in embryonic and postnatal brain. Further, we demonstrate that application of CMTMR facilitates the labeling of a large number of migrating cells and enables the characterization of their phenotypes with immunohistochemical and in situ hybridization techniques. We also illustrate that CMTMR labeling is ideally suited for time-lapse imaging of the behavior and dynamics of migrating cells.
Alifragis
Pavlos
P
Department of Anatomy and Developmental Biology, University College London, London, WC1E 6BT, United Kingdom.
Parnavelas
John G
JG
Nadarajah
Bagirathy
B
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D002452:Cell Count; D002465:Cell Movement / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008853:Microscopy / Q000379:methods; D008863:Microspheres; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D010641:Phenotype; D051381:Rats; D012235:Rhodamines / Q000737:chemistry; D013997:Time Factors; D014414:Tungsten / Q000737:chemistry
C116572:(((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine; D012235:Rhodamines; D014414:Tungsten