[Role of recombinant human bone morphogenetic protein 2/collagen as an onlay bone graft on adult rat calvarial bone].
OBJECTIVE
To evaluate the bone regenerative potential of recombinant human bone morphogenetic protein 2(rhBMP-2)/collagen on adult rat calvarial bone.


METHODS
A tight subperiosteal pocket was produced under both sides of the temporal muscle in rats. rhBMP-2/collagen was implanted in one side and collagen alone was implanted in the other side as control. The rats were sacrificed 2, 4 and 8 weeks after operation. The specimen was harvested and examined histologically. For morphometric analysis, the thickness of the temporal bone of both sides was measured and compared.


RESULTS
The rhBMP-2/collagen onlay implant resulted in active bone formation and the augmented bone was connected directly with the original bone, whereas the collagen alone resulted in neither bone nor cartilage production. The ossification process in the rhBMP-2/collagen occurred directly through bone formation, similar to intramembranous ossification.


CONCLUSIONS
rhBMP-2/collagen is an effective material as a biological onlay implant.
Chen
Gang
G
Department of Stomatology, Shenzhen Central Hospital, Shenzhen, Guangdong, P. R. China 518036. chg0425@sian.com.cn
Zhao
Shi-Jie
SJ
Yu
Guang-Yan
GY
Wang
hui-Xing
hX
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D016025:Bone Transplantation; D003094:Collagen / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D013701:Temporal Bone / Q000601:surgery; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen