title: Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro.
abstract: The mechanisms underlying the visual assessment and selection of immature oocytes resulting in optimum embryonic development following in vitro maturation, fertilization and culture (in vitro maturation (IVM)/in vitro fertilization (IVF)/in vitro embryo culture (IVC)) are unknown. Also, the reasons for the more frequent occurrence of cytoplasmic fragmentation in in vitro produced bovine embryos, resulting in poor survival following cryopreservation and decreased pregnancy rates following embryo transfer are not clear. The objectives of this study are: (1) to investigate whether differences in the quality of immature oocytes and embryo fragmentation are associated with apoptosis; and (2) to study the pattern of Bcl-2 and Bax expression in oocytes and embryos to help elucidate their potential roles in the regulation of apoptosis during development. Bovine oocytes were obtained from slaughterhouse ovaries and divided into four grades (grades I-IV) based on their morphology. Oocytes of different grades were cultured in serum-free medium for 48h. Embryos were produced only from grade I oocytes (highest quality) via IVM, IVF and IVC procedures. The morphological analysis of apoptosis in oocytes and embryos was carried out using propidium iodide staining and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling. The expression of Bcl-2 and Bax in oocytes and embryos of different qualities and stages was determined using western blotting. The results showed that the number of morphologically abnormal oocytes with shrinkage and/or fragmentation of the ooplasm, which are typical features of apoptosis, was significantly higher in grade IV oocytes (denuded oocytes, the lowest quality) than in grade I oocytes after 48h in vitro culture (P<0.05). DNA fragmentation, a hallmark of the biochemical changes seen in apoptotic cell death, was observed in morphologically fragmented oocytes and embryos. The expression of Bcl-2 was high in good quality oocytes and embryos, low in fragmented embryos, and hardly detectable in denuded oocytes. In contrast, the expression of Bax was found in all types of oocytes and embryos with the highest expression in the denuded oocytes. This implies that the ratio of Bcl-2 to Bax may be used to gauge the tendency of oocytes and embryos towards either survival or apoptosis. Overall, our results show that apoptosis appears to be an underlying mechanism of bovine oocyte degeneration and embryo fragmentation. Interactions between the Bcl-2 family of proteins may play a critical role in pre-implantation embryo development. These findings could have important implications for improving IVF and related techniques.
authors.affiliation: Department of Animal Science, University of British Columbia, Suite 208-2357 Main Hall, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Ming Yuan
authors.initials: MY
authors.lastname: Yang
authors.forename: Rajadurai
authors.initials: R
authors.lastname: Rajamahendran
mesh_terms: D000818:Animals; D017209:Apoptosis; D001755:Blastocyst / Q000502:physiology; D015153:Blotting, Western; D002417:Cattle / Q000196:embryology*; D002970:Cleavage Stage, Ovum; D015925:Cryopreservation; D046508:Culture Techniques; D053938:DNA Fragmentation; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D020287:In Situ Nick-End Labeling; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis* / Q000502:physiology; D051028:bcl-2-Associated X Protein
chemical_list: D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein