Spontaneous hybridization of macrophages and Meth A sarcoma cells.
We present evidence of hybridization between Meth A sarcoma cells and syngeneic as well as semigeneic peritoneal macrophages. The resultant hybrids are characterized by morphology, membrane markers, ploidy, chromosomal content and functional features. Briefly, after a few days of coculture, cells appeared with morphology intermediate between the 2 original cell types. Typical macrophage surface molecules appeared in the hybrids. Meth A cells were labeled with red fluorescence and macrophages with green fluorescence. After 4 days in vitro, hybrids with yellow fluorescence appeared. Macrophages from BALB.K mice (H-2 K(k)) were cocultivated with Meth A cells from BALB/c mice (H-2 K(d)). The semigeneic hybrids displayed both specificities, as demonstrated by flow cytometry. The hybrids appeared moderately phagocytic, less so than the macrophages and markedly more so than the essentially nonphagocytic Meth A cells. The hybrids had a mean number of 76 chromosomes, as opposed to 53 in the Meth A cells and 40 in the macrophages. The macrophage DNA index was set at 1; Meth A cells were found to have an index of 1.6 in G1 phase, and the hybrids had a 2.6 index. The hybrids grew more slowly in vitro than Meth A cells, but grew faster in vivo.
Busund
Lill-Tove R
LT
Department of Clinical Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway. busund@ozemail.com.au
Killie
Mette K
MK
Bartnes
Kristian
K
Olsen
Randi
R
Seljelid
Rolf
R
D000782:Aneuploidy; D000818:Animals; D002455:Cell Division; D018920:Coculture Techniques; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D006822:Hybrid Cells / Q000166:cytology / Q000648:ultrastructure; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000648:ultrastructure; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D010587:Phagocytosis; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000648:ultrastructure
D008748:Methylcholanthrene; D004247:DNA