[Effect of acrylonitrile on the activity of Ca(2+)-ATPase and phosphorylase A of liver in rats].
The activity of Ca(2+)-ATPase and phosphorylase A(P-A) of liver in rats was determined to study the effect of acrylonitrile (ACN) on calcium homeostasis and to clarify the toxicological mechanism of ACN. Adult male Sprague-Dawley rats were administered ACN daily per os for 42 days, at the dosage of 0, 10, 30 and 50 mg.kg-1. The activities of Ca(2+)-ATPase and phosphorylase A was determined at 14, 28, 42 days after ACN administration. The results indicated that ACN could significantly inhibit the activity of Ca(2+)-ATPase and increase the activity of P-A (P < 0.01), especially in the high dose group and exposed for 42 days, and there was significantly dose- and time-respond relationship (P < 0.01). ACN contamination could result in the disharmony of calcium homeostasis of liver in rats.
Zhang
Z
Z
Laboratory of Molecular Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Wang
X
X
Zhou
J
J
Gao
S
S
Jin
X
X
D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010762:Phosphorylase a / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D010762:Phosphorylase a; D000252:Calcium-Transporting ATPases; D000181:Acrylonitrile