Development of a rat C6 brain tumor model.
OBJECTIVE
To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume.


METHODS
C6 cell suspension (10 microl) containing 10 g/L agarose and 1 x 10(6) cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains.


RESULTS
Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%.


CONCLUSIONS
A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.
Zhang
Xiang
X
Neurosurgical Institute of PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China. xzhang@fmmu.edu.cn
Wu
Jingwen
J
Gao
Dakuan
D
Fei
Zhou
Z
Qu
Yan
Y
Jing
Junjie
J
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology; D004195:Disease Models, Animal; D005910:Glioma / Q000473:pathology; D008279:Magnetic Resonance Imaging; D051381:Rats