Modulatory effect of phenolic fractions of Terminalia arjuna on the mutagenicity in Ames assay.
We determined the antimutagenicity of phenolic fractions of Terminalia arjuna (soluble and insoluble in chloroform) against two direct-acting mutagens, 4-nitro-o-phenylenediamine (NPD) and sodium azide, and against the S9-dependent mutagen 2-aminofluorene (2AF), in TA98 and TA100 tester strains of Salmonella typhimurium. We found that the phenolic fractions of T. arjuna inhibited revertants induced by the S9-dependent mutagen more remarkably than the direct-acting mutagens. Furthermore, the phenolic fractions showed maximum inhibition of 98% and 101.55%, respectively, in the pre-incubation mode of treatment against the mutations induced by 2AF. Overall, the fractions inhibited the revertants induced by S9-dependent mutagens more effectively than those induced by direct-acting mutagens. The percentage of inhibition was higher in the pre-incubation than with direct acting mutagens. The fraction insoluble in chloroform showed more inhibition than the soluble one, which corresponds to a higher polyphenol content in the insoluble fraction than in the soluble extract.
Kaur
Kamaljit
K
Department of Botanical Sciences, Guru Nanak Dev University, Amritsar, India. kamal_rajput@usa.net
Arora
Saroj
S
Kumar
Subodh
S
Nagpal
Avinash
A
D002273:Carcinogens / Q000633:toxicity*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D010636:Phenols / Q000737:chemistry; D010655:Phenylenediamines / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D019810:Sodium Azide / Q000633:toxicity*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D029766:Terminalia / Q000737:chemistry*
D002273:Carcinogens; D004791:Enzyme Inhibitors; D010636:Phenols; D010655:Phenylenediamines; D010936:Plant Extracts; D020011:Protective Agents; D012997:Solvents; C012277:1,2-diamino-4-nitrobenzene; D019810:Sodium Azide