Thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry.
Four aerobic, gram-negative bacterial strains isolated from kaolin slurry used in the production of paper were subjected to a polyphasic analysis and characterization to determine their taxonomic position. Analysis of the 16S rDNA sequences of the four strains revealed that they represent a new lineage within the gamma-Proteobacteria, related to the genera Xanthomonas, Pseudoxanthomonas, Stenotrophomonas, Luteimonas, Xylella and Rhodanobacter. Analysis of the quinone system, the polyamines, the fatty acids and the polar lipids revealed a combination of characteristics that is unique and not described for the phylogenetic relatives. The four strains contain a ubiquinone Q-8, spermidine as the major polyamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the predominant polar lipids, and a fatty acid profile with predominantly iso-branched fatty acids. The G+C content of the genomic DNA was determined to be within the narrow range 67.1-68.7 mol%. Determination of DNA relatedness, as well as riboprint band patterns and amplified fragment length polymorphism profiles, clearly demonstrated that the four strains are members of a single species. Antibiotic-susceptibility patterns were identical for the four strains. Although showing a high degree of similarites in physiological and biochemical patterns, each of the four strains could be distinguished from the others on the basis of a few biochemical characteristics. On the basis of the estimates of phylogenetic relationships derived from the 16S rDNA sequence analyses, the observed chemotaxonomic characteristics and other phenotypic traits, a new genus, Thermomonas gen. nov., and species, Thermomonas haemolytica sp. nov., are proposed for the strains A50-7-3T (= DSM 13605T = LMG 19653T), B 50-7-1 (= DSM 13598 = LMG 19655), D50-7-1 (= DSM 13610 = LMG 19656) and B50-8-1 (= DSM 13599 = LMG 19654), with strain A50-7-3T as the type strain.
Busse
H J
HJ
Kämpfer
P
P
Moore
E R B
ERB
Nuutinen
J
J
Tsitko
I V
IV
Denner
E B M
EBM
Vauterin
L
L
Valens
M
M
Rosselló-Mora
R
R
Salkinoja-Salonen
M S
MS
D001482:Base Composition; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000737:chemistry; D020563:Gammaproteobacteria / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D007616:Kaolin; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D010209:Paper; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004269:DNA, Bacterial; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D007616:Kaolin