[Determination of lactitol in mice serum by high performance liquid chromatography].
An HPLC method for the determination of lactitol in mice serum was developed. A liquid chromatograph equipped with a refractive index detector and a Waters Sugar-Pakl column was used. The column temperature was maintained at 90 degrees C and the mobile phase was redistilled water. One milliliter of the serum sample was treated with 3.0 mL methanol. The precipitate was centrifuged for 15 min at 10,000 r/min. Two milliliters of the supernatant was dried on a water bath at 100 degrees C. The residue was dissolved with 1.0 mL water and 10 microL of which was injected. The average recovery was 98.6%. The detection limit was 0.1 microgram (S/N = 3.75). The linear range was 0-600 mg/L (r = 0.9992). Both the RSD's within day and between days were below 1.0% (n = 5).
Ni
W
W
Zhejiang Provincial Institute for Drug Control, Hangzhou, 310004.
Zhang
M
M
Huang
Z
Z
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D013402:Sugar Alcohols / Q000097:blood*
D013402:Sugar Alcohols; C014635:lactitol