Effect of Mahonia aquifolium active compounds on interleukin-8 production in the human monocytic cell line THP-1.
The effect of the crude extract and of two alkaloid fractions prepared from Mahonia aquifolium on interleukin-8 (IL-8) production in the lipopolysaccharide (LPS)-stimulated human monocytic cell line THP-1 was studied. The production of IL-8 by cells stimulated with 20 ng/ml LPS after 48 h treatment with 20 microg/ml crude extract was inhibited by about 30 %. LPS-stimulated cells treated with 0.1 microg/ml bisbenzylisoquinoline alkaloid fraction exhibited a 40% inhibition of IL-8 production in comparison with control cells. The fraction of protoberberine alkaloids had no significant inhibitory activity. Weak or no inhibition of IL-8 production was found after treatment with 0.5 microg/ml of protoberberine alkaloids berberine and jatrorrhizine and with berbamine from the group of BBI alkaloids. In contrast, the production was inhibited after treatment with BBI alkaloids baluchistine (about 20%) and aromoline (up to 30%).
Hajnická
Valéria
V
Kost'álová
Daniela
D
Svecová
Danka
D
Sochorová
Ruzena
R
Fuchsberger
Norbert
N
Tóth
Jaroslav
J
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044182:Benzylisoquinolines; D001599:Berberine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007546:Isoquinolines / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028143:Mahonia; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D000470:Alkaloids; D044182:Benzylisoquinolines; D016209:Interleukin-8; D007546:Isoquinolines; D010936:Plant Extracts; C458699:baluchistine; C055785:jatrorrhizine; D001599:Berberine; C066341:aromoline; C027870:berbamine