Analysis of gene expression in rose petals using expressed sequence tags.
Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indicated that transcripts of several of the genes identified accumulated specifically in petals and stamens. The cDNA library and expressed sequence tag database described here represent a valuable resource for future research aimed at improving economically important rose characteristics such as flower form, longevity and scent.
Channelière
Stéphanie
S
Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Cedex 07, Lyon, France.
Rivière
Stéphane
S
Scalliet
Gabriel
G
Szecsi
Judit
J
Jullien
Frédéric
F
Dolle
Caroline
C
Vergne
Philippe
P
Dumas
Christian
C
Bendahmane
Mohammed
M
Hugueney
Philippe
P
Cock
J Mark
JM
D020451:Contig Mapping; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015723:Gene Library; D018514:Plant Structures / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027826:Rosa
D018744:DNA, Plant; D018749:RNA, Plant