title: [Tartrate-resistant acid phosphatases (TRAP) and cytology of pseudosynovial membranes].
abstract: Pseudo-synovial membranes are found at the interface between bone and aseptically lost prostheses. They are supposed to be due to the release of material debris at the interface with bone. They are mostly composed of macrophages and giant cells, and some fibrocytes in the deep layer of the membrane. The different histological structures present in these membranes have been largely described. Very recently, Kadoya et al. have demonstrated that a lot of giant cells present in the pseudo-synovial membranes show a tartrate resistant acid phosphatase activity in the presence of more than 100 mM tartrate. They have also shown that some of these cells were responsible for bone degradation. We have studied the tartrate acid phosphatase activity of a series of 10 membranes containing various material debris: titanium alloy, stainless steel, polymethylmethacrylate, polyethylene. Many mono or multinucleated cells showed a TRAP activity in their lysosomes in the presence of a 400 mM tartrate concentration. In certain membranes, these cells showed a TRAP activity located in the nucleus. This study shows that the macrophage population is very heterogeneous and the difference between osteoclasts and giant cells may be unclear.
authors.affiliation: Sce de Biologie Buccale, Ecole Dentaire, Université Paul Sabatier Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Frayssinet
authors.forename: L
authors.initials: L
authors.lastname: Gineste
authors.forename: I
authors.initials: I
authors.lastname: Primout
authors.forename: A
authors.initials: A
authors.lastname: Guilhem
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D013583:Synovial Membrane / Q000166:cytology* / Q000201:enzymology*; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
vernacular_title: Phosphatases acides tartrate résistantes (TRAP) et cytologie des membranes pseudo-synoviales.