[Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells].
The effect of pp60c-src on mitogen-activated protein kinase (MAPK) activation induced by angiotensin II (Ang II) in vascular smooth muscle cells (VSMCs) was observed to explore the signal transduction process of VSMCs proliferation mediated by Ang II. Following transfection of the synthetic antisense c-src  oligodeoxynucleotides (ODNs) wrapped in lipofectin into cultured rat VSMCs, pp60c-src content markedly decreased, pp60c-src kinase activity was only  14.4% of control, while MAPK activity was not significantly changed (P>0.05). When these transfected VSMCs were further stimulated by Ang II, the increase in MAPK activity was only 1.6% of control. These data suggest that pp60c-src is needed for MAPK activation in VSMCs stimulated by Ang II, and pp60c-src may be an important signaling molecule in abnormal proliferation of VSMCs.
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Department of Cardiology, The Second Affiliated Hospital to Zhejiang University Medical School, Hangzhou 310006. liuding99cn@yahoo.com.cn
Lu
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Yin
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D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology; D002478:Cells, Cultured; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000804:Angiotensin II; D016392:Proto-Oncogene Proteins pp60(c-src); D020928:Mitogen-Activated Protein Kinases