The level of 8-oxo-7,8-dihydro-2'-deoxyguanosine is positively correlated with the size of the labile iron pool in human lymphocytes.
It appears that the labile iron pool (LIP, low molecular weight iron) presence in cells can result in the production of reactive oxygen species (ROS). ROS may be responsible for the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in cellular DNA. In the present study we report on the relationship between LIP and the endogenous level of 8-oxodGuo in human lymphocytes. Good correlation has been determined between LIP and the oxidatively modified nucleoside. This in turn points out the possibility that under physiological condition there is the availability of LIP for catalyzing Fenton-type reactions in close proximity to cellular DNA. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0335-x.
Gackowski
Daniel
D
Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, ul. Karlowicza 24, 85-092 Bydgoszcz, Poland.
Kruszewski
Marcin
M
Bartlomiejczyk
Teresa
T
Jawien
Arkadiusz
A
Ciecierski
Marek
M
Olinski
Ryszard
R
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001205:Ascorbic Acid / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood*; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood*; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D003849:Deoxyguanosine; D001205:Ascorbic Acid