Lack of association between atopic eczema/dermatitis syndrome and polymorphisms in the promoter region of RANTES and regulatory region of MCP-1.
BACKGROUND
Chemokines play an important role in the pathophysiology of atopic eczema/dermatitis syndrome (AEDS) and allergy. Recently polymorphisms in the promoter region of RANTES (regulated on activation normal T cell expressed and secreted) and in the gene regulatory region of MCP-1 (monocyte chemoattractant protein-1) have been found, which increase the expression of these chemokines. The - 403A allele of the RANTES promoter region was found associated with AEDS in German children. We investigated whether the presence of these polymorphisms was associated with AEDS or allergy in Hungarian children.


METHODS
One hundred and twenty-eight children with AEDS, 102 allergic children without AEDS and 303 children of comparable ages without allergic disorders were screened for genotype with a PCR-based assay.


RESULTS
There were no significant differences in the frequency of these polymorphisms, or in the distribution of genotypes between the groups. The total IgE concentration, the white blood cell count and the blood eosinophil cell count did not differ between the genotypes.


CONCLUSIONS
In this cohort of Hungarian children there was no association between - 28G, and - 403A alleles in the RANTES promoter, - 2518G polymorphism in the distal regulatory region of the MCP-1 and AEDS, or allergy.
Kozma
G T
GT
Department of Genetics, Cell- and Immunobiology, Semmelweis University Budapest, Budapest, Hungary.
Falus
A
A
Bojszkó
A
A
Krikovszky
D
D
Szabó
T
T
Nagy
A
A
Szalai
C
C
D000293:Adolescent; D018932:Chemokine CCL2 / Q000235:genetics*; D018946:Chemokine CCL5 / Q000235:genetics*; D002648:Child; D002669:Child Welfare; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D004804:Eosinophils; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D015995:Prevalence; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013223:Statistics as Topic
D018932:Chemokine CCL2; D018946:Chemokine CCL5; D007073:Immunoglobulin E