Rapid high-performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor.
A rapid high-performance liquid chromatographic method using a C(18) reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO(4) small middle dot7H(2)O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH(3)CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H(3)PO(4). Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays.
Maltese
Adriana
A
Fidia Oftal Research Laboratories, C.so Italia 141, 95127 Catania, Italy.
Bucolo
Claudio
C
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013449:Sulfonamides / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D004791:Enzyme Inhibitors; D013449:Sulfonamides; D013876:Thiophenes; C062765:dorzolamide