Conservation of Brachyury, Mef2, and Snail in the myogenic lineage of jellyfish: a connection to the mesoderm of bilateria.
One major difference between simple metazoans such as cnidarians and all the bilaterian animals is thought to involve the invention of mesoderm. The terms diploblasts and triploblasts are therefore, often used to group prebilaterian and bilaterian animals, respectively. However, jellyfish contain well developed striated and smooth muscle tissues that derive from the entocodon, a mesoderm-like tissue formed during medusa development. We investigated the hypothesis, that the entocodon could be homologous to the third germ layer of bilaterians by analyzing the structures and expression patterns of the homologues of Brachyury, Mef2, and Snail in the jellyfish Podocoryne carnea. These are regulatory genes from the T-box, MADS-box and zinc finger families known to play important roles in bilaterian mesoderm patterning and muscle differentiation. The sequence and expression data demonstrate that the genes are structurally and functionally conserved and even more similar to humans or other deuterostomes than to protostome model organisms such as Drosophila or Caenorhabditis elegans. Based on these data we conclude that the common ancestor of the cnidarians and bilaterians not only shared genes that play a role in regulating myogenesis but already used them to develop and differentiate muscle systems similar to those of triploblasts.
Spring
Jürg
J
Institute of Zoology, Biocenter/Pharmacenter, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland.
Yanze
Nathalie
N
Jösch
Christoph
C
Middel
Arnoud M
AM
Winninger
Brigitte
B
Schmid
Volker
V
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005326:Fetal Proteins; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D064326:MEF2 Transcription Factors; D008648:Mesoderm / Q000502:physiology*; D009024:Morphogenesis; D018006:Myogenic Regulatory Factors; D009928:Organ Specificity; D010802:Phylogeny; D037401:Scyphozoa / Q000145:classification / Q000196:embryology* / Q000235:genetics; D000071250:Snail Family Transcription Factors; D020825:T-Box Domain Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D000098908:Brachyury Protein
D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D064326:MEF2 Transcription Factors; D018006:Myogenic Regulatory Factors; D000071250:Snail Family Transcription Factors; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D000098908:Brachyury Protein; C000604451:sna protein, Drosophila