title: Biodiversity (Communications arising): maize transgene results in Mexico are artefacts.
abstract: Quist and Chapela's conclusion that the transgenes they claim to have detected in native maize in Oaxaca, Mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-PCR) they used to amplify sequences flanking 35S transgenes from cauliflower mosaic virus (CaMV).
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA. nkaplins@nature.berkeley.edu
authors.forename: Nick
authors.initials: N
authors.lastname: Kaplinsky
authors.forename: David
authors.initials: D
authors.lastname: Braun
authors.forename: Damon
authors.initials: D
authors.lastname: Lisch
authors.forename: Angela
authors.initials: A
authors.lastname: Hay
authors.forename: Sarah
authors.initials: S
authors.lastname: Hake
authors.forename: Michael
authors.initials: M
authors.lastname: Freeling
mesh_terms: D016477:Artifacts; D017931:DNA Primers / Q000235:genetics; D018745:Genome, Plant; D007438:Introns / Q000235:genetics; D008800:Mexico; D009693:Nucleic Acid Hybridization; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D018626:Retroelements / Q000235:genetics*; D019076:Transgenes / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018626:Retroelements