Effect of safrole oxide on vascular endothelial cell growth and apoptosis induced by deprivation of fibroblast growth factor.
OBJECTIVE
To investigate effect of safrole oxide on cell growth and apoptosis induced by deprivation of survival factors (fibroblast growth factors, aFGF and bFGF) in vascular endothelial cells (VEC).


METHODS
Morphological changes were observed by light microscopy. Cell growth was determined by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium) method. DNA fragmentation was analyzed by agarose gel electrophoresis and fluorescence microscopy. Cell cycle distribution was analyzed by flow cytometry (FCM).


RESULTS
The cells deprived of FGF were exposed to safrole oxide 5-25 mg/L for 24 h. Cells spreading and growth were promoted (P<0.01), detachment and DNA fragmentation of these cells were suppressed (P<0.01), safrole oxide 10 mg/L had no obvious effect on cell cycle distribution (P>0.05). When the cells were treated with safrole oxide 50-100 mg/L, detachment and DNA fragmentation of VEC were promoted (P<0.01). The cell cycle was blocked at G2-M phase by safrole oxide 100 mg/L.


CONCLUSIONS
Safrole oxide 10 mg/L inhibited, but 100 mg/L promoted apoptosis of VEC. Safrole oxide might be an important compound that affects VEC growth and apoptosis.
Miao
Jun-Ying
JY
Institute of Developmental Biology, School of Life Science, Shandong University, Ji-nan 250100, China. Miaojy@jn-public.sd.cninfo.net
Zhao
Bao-Xiang
BX
Li
Hui-Hui
HH
Zhang
Shang-Li
SL
Du
Chun-Qing
CQ
D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006801:Humans; D012451:Safrole / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D005346:Fibroblast Growth Factors; C009196:safrole oxide; D012451:Safrole