Entrapment of proteins and DNA in thermally evaporated lipid films.
The immobilization of biomacromolecules such as proteins, enzymes and DNA in various inert matrices is a problem that attracts considerable attention and is motivated by fundamental, biomedical and industrial interests. In addition to several other entrapping matrices, lipids in the form of monolayers and bilayers are versatile hosts owing to their membrane-mimicking capability, bio-friendliness, flexibility and inertness. Here, we discuss the immobilization of proteins, enzymes and DNA via electrostatic interactions in films of thermally evaporated fatty lipids. The role of the lipid in preserving the natural conformation of the biomolecule, protection against harsh environmental conditions and accessibility to substrates and reagents is an important feature of the protocol and is highlighted.
Sastry
Murali
M
Materials Chemistry Division, National Chemical Laboratory, 411 008, Pune, India. sastry@ems.ncl.res.in
D000588:Amines / Q000737:chemistry; D018441:Biofilms; D015374:Biosensing Techniques; D002480:Cellulase / Q000032:analysis* / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000032:analysis / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008563:Membrane Lipids / Q000378:metabolism* / Q000502:physiology; D008567:Membranes, Artificial; D008956:Models, Chemical; D011485:Protein Binding / Q000502:physiology; D055672:Static Electricity; D013329:Structure-Activity Relationship
D000588:Amines; D004800:Enzymes, Immobilized; D046911:Macromolecular Substances; D008563:Membrane Lipids; D008567:Membranes, Artificial; D004247:DNA; D002480:Cellulase; C009317:stearylamine