Hepatic lysosomal enzymes activity and liver morphology after short-time omeprazole administration.
The aim of the study was to establish the influence of short-time omeprazole administration on liver function and morphology. Omeprazole was administered intraperitoneally, twice daily, for 3 days to male Wistar rats in two doses: 0.571 mg/kg and 5.71 mg/kg. Control animals were treated with physiological saline. Half of the animals were sacrificed 12 hours after the last injection. The remaining rats were raised for another 6 weeks, without any xenobiotics, and sacrificed on the 47th day of the experiment. The activity of free and bound fractions of hepatic acid phosphatase, beta-galactosidase, beta-N-acetyl-glucosaminidase, cathepsin B, D and L, lipase, and sulphatase were determined spectrophotometrically in homogenates of the liver. The liver sections were examined by light microscopy with hematoxylin-eosin, azan, and periodic acid-Schiff stains. Marginally significant (p < 0.1) differences in activity of free sulphatase fraction, and free and bound fractions of beta-galactosidase were found in animals exposed to the higher dose of omeprazole and sacrificed 12 hours after the last injection. Enzymatic profiles were normalised during the next 6 weeks. Histological evaluation revealed small degenerative and adaptive changes in all examined groups. It could be concluded that observed differences of hepatic lysosomal enzyme activities were the result of accompanied chemical-induced peritonitis as previously reported, and not a direct drug-toxic effect.
Burdan
Franciszek
F
Human Anatomy Department, Medical University of Lublin, Poland.
Siezieniewska
Zofia
Z
Maciejewski
Ryszard
R
Madej
Barbara
B
Radzikowska
Elzbieta
E
Wojtowicz
Zbigniew
Z
D000818:Animals; D021461:Animals, Outbred Strains; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009336:Necrosis; D009853:Omeprazole / Q000008:administration & dosage / Q000633:toxicity*; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D017208:Rats, Wistar; D013429:Sulfatases / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D004791:Enzyme Inhibitors; D013429:Sulfatases; D001616:beta-Galactosidase; D009853:Omeprazole