Diversity and phylogenetic analysis of bacteria in the mucosa of chicken ceca and comparison with bacteria in the cecal lumen.
We reported the first attempt to describe mucosa-associated bacterial populations in the chicken ceca by molecular analysis of 16S rRNA genes. Bacteria in the mucosa were highly diverse but mainly Gram-positive with low G+C. Fusobacterium prausnitzii and butyrate-producing bacteria comprised the largest groups among 116 cloned sequences. Twenty five percent of the clones had less than 95% homology to database sequences. Many sequences were related to those of uncultured bacteria identified in human feces or the bovine rumen. Terminal restriction fragment length polymorphism (T-RFLP) analysis revealed some differences between bacterial populations present in the mucosa and lumen of ceca. Greater resolution of bacterial population was obtained using a culture-independent approach rather than a culture-based approach.
Gong
Jianhua
J
Food Research Program, Agriculture and Agri-Food Canada, N1G 5C9, Guelph, ON, Canada. gongj@em.agr.ca
Forster
Robert J
RJ
Yu
Hai
H
Chambers
James R
JR
Sabour
Parviz M
PM
Wheatcroft
Roger
R
Chen
Shu
S
D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media; D017753:Ecosystem; D020459:Genes, rRNA; D014644:Genetic Variation; D007413:Intestinal Mucosa / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D003470:Culture Media; D012336:RNA, Ribosomal, 16S