title: Molecular analysis of an unusual rearrangement between chromosomes 4 and 11 in adult pre-B-cell acute lymphoblastic leukemia.
abstract: The reciprocal t(4;11)(q21;q23) is often described in acute lymphoblastic leukemia (ALL). In some cases, variant or complex t(4;11) has been detected in ALL by cytogenetic analysis, but to our knowledge no molecular study has been reported in these variant translocations. We describe a 27-year-old woman suffering from pre-B-cell ALL with an unusual rearrangement between chromosomes 4 and 11. Because this complex rearrangement involved the 11q23 chromosome band known to be associated with poor prognosis, we performed fluorescence in situ hybridization, Southern blot, and reverse transcription polymerase chain reaction analyses. This confirmed myeloid lymphoid leukemia gene rearrangement and showed the presence of MLL/AF4 fusion transcript. These results showed the importance of molecular analysis that allowed minimal residual disease monitoring in this patient.
authors.affiliation: Laboratoire d'Hématologie, Hôpital Henri Mondor, 94010 Créteil Cedex, France.
authors.forename: Ibtissam
authors.initials: I
authors.lastname: Chami
authors.forename: Christine
authors.initials: C
authors.lastname: Pérot
authors.forename: Marie France
authors.initials: MF
authors.lastname: Portnoï
authors.forename: Hélène
authors.initials: H
authors.lastname: Jouault
authors.forename: Claire
authors.initials: C
authors.lastname: Rieux
authors.forename: Cécile
authors.initials: C
authors.lastname: Pautas
authors.forename: Mathieu
authors.initials: M
authors.lastname: Kuentz
authors.forename: Michel
authors.initials: M
authors.lastname: Tulliez
authors.forename: Dominique
authors.initials: D
authors.lastname: Bories
mesh_terms: D000328:Adult; D015139:Blotting, Southern; D002869:Chromosome Aberrations; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D051788:Myeloid-Lymphoid Leukemia Protein; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; C113855:MLL-AF4 fusion protein, human; D015514:Oncogene Proteins, Fusion; D051788:Myeloid-Lymphoid Leukemia Protein