title: In vivo temperature heterogeneity of atherosclerotic plaques is determined by plaque composition.
abstract: BACKGROUND
  Temperature heterogeneity of atherosclerotic plaques has been associated with macrophage accumulation in ex vivo studies. We investigated in vivo whether modifying the cell composition of rabbit atherosclerotic plaques by dietary cholesterol lowering can influence temperature heterogeneity.
  
  
  RESULTS
  Twenty New Zealand rabbits were randomized to either a normal (n=10) or cholesterol-rich (0.3%) diet (n=10) for 6 months. Thereafter, intravascular ultrasound and intravascular catheter-based thermography of the surface of aortic arch and descending aorta were performed in all animals. Ten control and 5 hypercholesterolemic rabbits were euthanized, and their aortas were analyzed histologically. The 5 remaining rabbits received a normal diet for 3 months and underwent repeat ultrasound and thermography before euthanasia followed by histology. Ex vivo temperature was measured in 3 additional rabbits at 6 months to correlate local temperature with local plaque composition. In control animals, plaque formation and temperature heterogeneity were absent. In hypercholesterolemic rabbits, plaque formation was prominent in the thoracic aorta. Plaques were composed of fibromuscular tissue and contained, underneath endothelial cells, an accumulation of foam cells of macrophage origin. Temperature heterogeneity was markedly elevated and increased with plaque thickness. Importantly, after 3 months of cholesterol lowering, plaque thickness remained unchanged, but temperature heterogeneity was significantly decreased. This paralleled plaque histology, which showed a marked loss of macrophages. The ex vivo experiments demonstrated the relation between local temperature and local total macrophage mass.
  
  
  CONCLUSIONS
  In vivo temperature heterogeneity of rabbit atherosclerotic plaques is determined by plaque composition. In vivo thermography may have important clinical implications in the assessment of plaque composition.
authors.affiliation: Cardiovascular Translational Research Institute Middelheim Antwerp, Belgium. stefan.verheye@pandora.be
authors.forename: Stefan
authors.initials: S
authors.lastname: Verheye
authors.forename: Guido R Y
authors.initials: GR
authors.lastname: De Meyer
authors.forename: Glenn
authors.initials: G
authors.lastname: Van Langenhove
authors.forename: Michiel W M
authors.initials: MW
authors.lastname: Knaapen
authors.forename: Mark M
authors.initials: MM
authors.lastname: Kockx
mesh_terms: D000818:Animals; D001018:Aortic Diseases / Q000000981:diagnostic imaging / Q000473:pathology; D001161:Arteriosclerosis / Q000000981:diagnostic imaging / Q000473:pathology*; D002404:Catheterization; D008078:Cholesterol, LDL / Q000097:blood; D004036:Diet, Atherogenic; D005487:Foam Cells; D006937:Hypercholesterolemia / Q000097:blood / Q000209:etiology; D008297:Male; D011817:Rabbits; D013696:Temperature; D013817:Thermography; D014463:Ultrasonography
chemical_list: D008078:Cholesterol, LDL