[Tumor necrosis factor-alpha enhances type I inositol 1, 4, 5-triphosphate receptor expression in rat glomerular afferent arterioles smooth muscle cells].
OBJECTIVE
To study the effect of tumor necrosis factor-alpha (TNF-alpha) on type I inositol 1, 4, 5-triphosphate receptor (IP(3) R) expression in rat glomerular afferent arterioles smooth muscle cells (RASMC).


METHODS
Isolation and culture of RASMC and detection of type I IP(3) R protein and type I IP(3) R mRNA in RASMC after TNF-alpha treatment were carried out with Western blot and Northern blot assay.


RESULTS
TNF-alpha enhanced the expression of type I IP(3) R protein and type I IP(3) R mRNA in RASMC; TNF-alpha did not influence the half life of type I IP(3) R mRNA in RASMC treated with TNF-alpha.


CONCLUSIONS
TNF-alpha plays a role in the development of renal dysfunction. The mechanisms may be that TNF-alpha takes part in the change of signal transduction in RASMC. TNF-alpha may act on the promoter of type I IP(3) R mRNA in RASMC and results in the expression of type I IP(3) R protein that stimulates release of intracellular Ca(2+) in RASMC and induces contraction of RASMC. The renal blood flow diminution leads to the development of renal dysfunction.
Wang
Jingyan
J
Department of Infectious Diseases, The Second Clinical College of China Medical University, Shenyang 110004, China.
Sun
Jinchun
J
Lu
Sa
S
Liu
Pei
P
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007678:Kidney Glomerulus / Q000098:blood supply; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014409:Tumor Necrosis Factor-alpha