title: Time-dependent changes in plasma concentrations of 3-methylindole and blood concentrations of 3-methyleneindolenine-adduct in feedlot cattle.
abstract: OBJECTIVE
  To describe time-dependent changes in plasma concentrations of 3-methylindole (3MI) and blood concentrations of 3-methyleneindolenine (3MEIN)-adduct in feedlot cattle.
  
  
  METHODS
  64 yearling steers.
  
  
  METHODS
  Steers were assigned to 2 groups (32 steers/group). During the first 8 weeks, blood samples were collected from group 1 before the morning ration was fed, whereas samples from group 2 were collected 2 to 3 hours after the ration was fed. Blood samples were collected from all steers approximately 4 times/wk for 3 weeks and 3 times/wk for the subsequent 5 weeks. Samples were collected at the same time for all steers for an additional 10 weeks. Plasma samples were analyzed for 3MI concentrations. Blood samples collected from cattle in group 2 during the first 8 weeks were analyzed for 3MEIN-adduct concentrations.
  
  
  RESULTS
  Mean blood concentration of 3MEIN-adduct increased to a maximum value on day 33 (0.80 U/microg of protein) and then decreased to a minimum on day 54 0.40 U/microg of protein). Plasma 3MI concentrations initially decreased and remained low until after day 54. Group-1 cattle had lower plasma 3MI concentrations, compared with concentrations for group-2 cattle. Blood 3MEIN-adduct concentrations and plasma 3MI concentrations were not associated with deleterious effects on weight gains.
  
  
  CONCLUSIONS
  Blood 3MEIN-adduct concentrations peaked during the period of greatest risk for development of bovine respiratory disease complex. Conversely, plasma 3MI concentrations decreased during the same period. Animal-to-animal variation in metabolic capacity to convert 3MI to 3MEIN may be of more importance than differences in plasma 3MI concentration.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
authors.forename: Guy H
authors.initials: GH
authors.lastname: Loneragan
authors.forename: Paul S
authors.initials: PS
authors.lastname: Morley
authors.forename: John J
authors.initials: JJ
authors.lastname: Wagner
authors.forename: Gary L
authors.initials: GL
authors.lastname: Mason
authors.forename: Garold S
authors.initials: GS
authors.lastname: Yost
authors.forename: Mike A
authors.initials: MA
authors.lastname: Thoren
authors.forename: Joni
authors.initials: J
authors.lastname: Triantis
mesh_terms: D000818:Animals; D001835:Body Weight; D002417:Cattle / Q000097:blood*; D007211:Indoles / Q000097:blood*; D008297:Male; D012862:Skatole / Q000097:blood*; D013997:Time Factors
chemical_list: D007211:Indoles; C097745:3-methyleneindolenine; D012862:Skatole