title: Electron transfer of hemoglobin at electrodes modified with colloidal clay nanoparticles.
abstract: Nanostructured sodium montmorillonite was prepared via a colloidal chemical approach and deposited onto glassy carbon electrodes (GCE). Subsequently, hemoglobin was spontaneously adsorbed onto the clay membrane-modified electrode. The colloidal clay nanoparticles and the adsorbed protein were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The electrochemical impedance behavior of the system was studied using a microlithographically fabricated interdigitated microsensor electrode (IME). The interaction of the clay nanoparticles with hemoglobin was investigated by UV-VIS spectroscopy and electrochemical methods. The heme protein adsorbed in this way displayed a well-defined electrode process and the electron transfer was confirmed to originate from its heme site. Furthermore, nitric oxide affects the hemoglobin electrochemistry.
authors.affiliation: Department of Analytical Biochemistry, University of Potsdam, Golm, Germany.
authors.forename: Chenghong
authors.initials: C
authors.lastname: Lei
authors.forename: Ulla
authors.initials: U
authors.lastname: Wollenberger
authors.forename: Nikitas
authors.initials: N
authors.lastname: Bistolas
authors.forename: Anthony
authors.initials: A
authors.lastname: Guiseppi-Elie
authors.forename: Frieder W
authors.initials: FW
authors.lastname: Scheller
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry; D000818:Animals; D001546:Bentonite; D015374:Biosensing Techniques / Q000379:methods; D000077215:Clay; D003102:Colloids / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D009569:Nitric Oxide / Q000494:pharmacology; D010084:Oxidation-Reduction
chemical_list: D000538:Aluminum Silicates; D003102:Colloids; D006454:Hemoglobins; D001546:Bentonite; D009569:Nitric Oxide; D000077215:Clay