title: [Tumor necrosis factor-alpha enhances type I inositol 1, 4, 5-triphosphate receptor expression in rat glomerular afferent arterioles smooth muscle cells].
abstract: OBJECTIVE
  To study the effect of tumor necrosis factor-alpha (TNF-alpha) on type I inositol 1, 4, 5-triphosphate receptor (IP(3) R) expression in rat glomerular afferent arterioles smooth muscle cells (RASMC).
  
  
  METHODS
  Isolation and culture of RASMC and detection of type I IP(3) R protein and type I IP(3) R mRNA in RASMC after TNF-alpha treatment were carried out with Western blot and Northern blot assay.
  
  
  RESULTS
  TNF-alpha enhanced the expression of type I IP(3) R protein and type I IP(3) R mRNA in RASMC; TNF-alpha did not influence the half life of type I IP(3) R mRNA in RASMC treated with TNF-alpha.
  
  
  CONCLUSIONS
  TNF-alpha plays a role in the development of renal dysfunction. The mechanisms may be that TNF-alpha takes part in the change of signal transduction in RASMC. TNF-alpha may act on the promoter of type I IP(3) R mRNA in RASMC and results in the expression of type I IP(3) R protein that stimulates release of intracellular Ca(2+) in RASMC and induces contraction of RASMC. The renal blood flow diminution leads to the development of renal dysfunction.
authors.affiliation: Department of Infectious Diseases, The Second Clinical College of China Medical University, Shenyang 110004, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Wang
authors.forename: Jinchun
authors.initials: J
authors.lastname: Sun
authors.forename: Sa
authors.initials: S
authors.lastname: Lu
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007678:Kidney Glomerulus / Q000098:blood supply; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014409:Tumor Necrosis Factor-alpha