[Enantiomeric separation of drugs with capillary electrophoresis with cyclodextrins].
General strategies for the development of capillary electrophoretic methods for the enantiomeric separation of basic, acidic or neutral drugs have been developed. For all kinds of compounds the use of a buffer made of 100 mM phosphoric acid adjusted to pH 3 or 5 with triethanolamine, containing cyclodextrin (CD) derivatives as chiral selectors, is recommended. Five b-cyclodextrin derivatives were found to be particularly useful for enantioseparations: dimethyl-beta-CD (DMCD), trimethyl-beta-CD (TMCD), hydroxypropyl-beta-CD (HPCD), carboxymethyl-beta-CD (CMCD) and sulfobutyl-beta-CD (SBCD). Two different strategies are proposed, depending on the acidic or basic character of the analytes. Using these strategies, 96% of the 50 drugs examined as model compounds could be fully enantioseparated, which confirmed the usefulness of capillary electrophoresis in order to obtain complete enantiomeric separation as quickly as possible and with a minimum consumption of chiral selector.
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D003505:Cyclodextrins; D019075:Electrophoresis, Capillary / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*
D003505:Cyclodextrins; D004364:Pharmaceutical Preparations
Séparation énantiomérique de médicaments par électrophorèse capillaire à l'aide de cyclodextrines.