[Effect of kidney tonifying and circulation promoting compound on transcription of T-cell apoptosis related Fas and FasL genes in aged rats].
OBJECTIVE
To probe the molecular mechanism for down-regulation of T-cell apoptosis by Kidney tonifying compound (KTC) in aged rats.


METHODS
GAPDH was used as an internal control gene, and semi-quantitative analysis of the transcription of Fas and FasL genes was performed by using RNase protection assay.


RESULTS
The Fas/GAPDH and FasL/GAPDH optical density (OD) ratios was 0.81 +/- 0.17 and 0.84 +/- 0.12 in the aged control group, and 0.36 +/- 0.16 and 0.26 +/- 0.12 in the young control group, the difference between the two groups was significant. In the circulation promoting group, the parameters were 0.99 +/- 0.36 and 0.94 +/- 0.33 which were not significantly different as compared with those in the aged control group (P > 0.05). In the two Kidney tonifying groups, the Fas/GAPDH OD ratio was 0.78 +/- 0.11 and 0.78 +/- 0.20, respectively, and no significant difference was observed as compared with those in the aged control group. However the FasL/GAPDH OD ratios in the two kidney tonifying group were 0.71 +/- 0.21 and 0.68 +/- 0.15, which were lower than those in the aged control group (P < 0.01).


CONCLUSIONS
The negative regulation of FasL gene expression may be one of the molecular mechanisms of KTC in down-regulating excessive apoptosis of T-cell in aged rats.
Zheng
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Z
Institute of Integrated Traditional Chinese and Western Medicine, Shanghai Medical University, Shanghai (200040).
Shen
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Zheng
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D000375:Aging / Q000235:genetics* / Q000276:immunology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013601:T-Lymphocytes / Q000473:pathology*; D014158:Transcription, Genetic; D019014:fas Receptor / Q000235:genetics*
D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; C505662:Faslg protein, rat; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor