Antibodies induced in mice by a DNA-construct coding for the elastase of Schistosoma mansoni recognize the enzyme in secretions and preacetabular glands of cercariae.
A DNA-construct coding for the elastase of the parasite Schistosoma mansoni was prepared from adult S. mansoni worm RNA which was reverse transcribed into cDNA. The gene coding for the elastase was amplified using primers specific for the sequence of cercarial elastase and was cloned into a mammalian expression vector. Expression of the elastase gene at the transcriptional level was achieved for the first time in transfected mammalian cells (COS-7) and was also successful in muscle tissue of mice injected with the DNA-construct. These mice developed antibodies recognizing in Western blots the elastase from cercarial secretions. Also, these antibodies reacted in immunofluorescence tests with the preacetabular glands of cercariae, i.e. the site of origin for elastase. Thus, the DNA-construct induced the expression of elastase in mice and formation of antibodies that recognized the native antigen.
Bahgat
M
M
Department of Tropical Hygiene, University of Heidelberg, Germany.
Chlichlia
K
K
Schirrmacher
V
V
Ruppel
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000276:immunology*; D001700:Biomphalaria / Q000469:parasitology; D015153:Blotting, Western; D019556:COS Cells; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology*; D005260:Female; D051379:Mice; D009693:Nucleic Acid Hybridization; D010196:Pancreatic Elastase / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D016053:RNA, Protozoan / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D014162:Transfection
D000913:Antibodies, Protozoan; D018076:DNA, Complementary; D016053:RNA, Protozoan; D011994:Recombinant Proteins; D010196:Pancreatic Elastase