[Congenital human cytomegalovirus infection: value of human cytomegalovirus DNA quantification in amniotic fluid].
A quantitative PCR assay (RS Elosa  CMV, Lambdatech) was used to quantitate HCMV DNA in maternal amniotic fluid of 12 fetuses with congenital infection (group 1) and of 10 fetuses without congenital infection (group 2). HCMV detection was performed for both groups using culture and qualitative PCR. Histologic examinations of fetal tissues and placenta were carried out for 9 patients from group 1. The amniotic fluid viral loads were negative in all patients of group 2. In group 1, all viral loads were high (from 1.105 to > 107 cop/mL) and no difference was observed between symptomatic and asymptomatic foetuses. Further evaluation on larger samples is needed to define more precisely the pronostic value of HCMV DNA quantification in amniotic fluid.
Nedelec
O
O
Service de virologie, CHRU de Lille, bâtiment Paul-Boulanger 59037 Lille, France.
Bellagra
N
N
Devisme
L
L
Hober
D
D
Wattré
P
P
Dewilde
A
A
D000653:Amniotic Fluid / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D019562:Viral Load
D004279:DNA, Viral
Infections congénitales à cytomégalovirus humain: valeur de la quantification de l'ADN du CMVH dans le liquide amniotique.