Glutathione peroxidase from the liver of Japanese sea bass Lateolabrax japonicus.
Glutathione peroxidase (EC 1.11.1.9) present in the liver of Japanese sea bass (Lateolabrax japonicus) was extracted and purified by phenyl-toyopearl 650M, butyl-toyopearl 650M and DEAE-toyopearl 650M column chromatography. The molecular weight of the enzyme was estimated to be about 70 kDa by gel filtration by toyopearl HW-55F. On SDS-PAGE, this enzyme was composed of two identical subunits with 35 kDa and was a dimer. This enzyme was a typical SH-enzyme that was inhibited by iodoacetic acid, PCMB, DTNB, and Hg.
Nagai
Takeshi
T
Department of Food Science and Technology, National Fisheries University, Shimonoseki, Yamaguchi, Japan. machin@fish-u.ac.jp
Yukimoto
Takakiyo
T
Suzuki
Nobutaka
N
D000818:Animals; D001492:Bass; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005979:Glutathione Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D007564:Japan; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D013816:Thermodynamics
D005979:Glutathione Peroxidase