title: Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry.
abstract: The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI-MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristic. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97-F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments.
authors.affiliation: Antibiotics Laboratory, RIKEN, 2-1 Hirosawa Wako, Saitama 351-0198, Japan.
authors.forename: Jeevan Kumar
authors.initials: JK
authors.lastname: Prasain
authors.forename: Masashi
authors.initials: M
authors.lastname: Ueki
authors.forename: Piotr
authors.initials: P
authors.lastname: Stefanowicz
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003572:Cytochalasins / Q000032:analysis*; D005285:Fermentation; D005658:Fungi / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003572:Cytochalasins