Human receptors for sweet and umami taste.
The three members of the T1R class of taste-specific G protein-coupled receptors have been hypothesized to function in combination as heterodimeric sweet taste receptors. Here we show that human T1R2/T1R3 recognizes diverse natural and synthetic sweeteners. In contrast, human T1R1/T1R3 responds to the umami taste stimulus l-glutamate, and this response is enhanced by 5'-ribonucleotides, a hallmark of umami taste. The ligand specificities of rat T1R2/T1R3 and T1R1/T1R3 correspond to those of their human counterparts. These findings implicate the T1Rs in umami taste and suggest that sweet and umami taste receptors share a common subunit.
Li
Xiaodong
X
Senomyx, Inc., 11099 North Torrey Pines Road, La Jolla, CA 92037, USA.
Staszewski
Lena
L
Xu
Hong
H
Durick
Kyle
K
Zoller
Mark
M
Adler
Elliot
E
D000595:Amino Acid Sequence; D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology; D018698:Glutamic Acid / Q000494:pharmacology*; D006157:Guanosine Monophosphate / Q000494:pharmacology; D006801:Humans; D007291:Inosine Monophosphate / Q000494:pharmacology; D008969:Molecular Sequence Data; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D013649:Taste / Q000502:physiology*; D014162:Transfection
D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; D007291:Inosine Monophosphate; D018698:Glutamic Acid; D006157:Guanosine Monophosphate; D019204:GTP-Binding Proteins