Bioimprinting of polymers and sol-gel phases. Selective detection of yeasts with imprinted polymers.
Coated quartz crystal microbalances were modified with a surface-imprinting process using whole yeast cells. These molded polymer and sol-gel surfaces show honeycomb-like structures as shown by atomic force microscopy. Reinclusion of cells allows a selective on-line monitoring of these microorganism concentrations in water over 5 orders of magnitude. The sensitivity to cells holds up in growth media up to 21 g/L. Even cell fragments can be detected in flowing conditions. The highly robust polymers on the sensor devices are suitable for biotechnological applications.
Dickert
F L
FL
Institute of Analytical Chemistry, University of Vienna, Austria. Franz.Dickert@univie.ac.at
Hayden
G
G
D005782:Gels / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D005782:Gels; D011108:Polymers