[Effect of scald on gene transcription and content of endothelin-1 in supraoptic nucleus of rat hypothalamus].
Endothelin-1 (ET-1) gene transcription and endothelin-1-immunoreactivity (ET-1-ir) in the supraoptic nucleus (SON) of rat hypothalamus were respectively observed by in situ hybridization and immunohistochemistry after scald. Intensity of ET-1 mRNA and endothelin-1-immunoreactivity (ET-1-ir) was quantified by image analysis. Compared with the control (sham scald) group, no significant change in the intensity of ET-1 mRNA positive hybridization signals in SON was found 15 min post-scald, while there was a 35.1% increase in the positive hybridization signal intensity 60 min post-scald (P<0.05) and a 62.4% increase 180 min post-scald (P<0.01). The content of ET-1-ir in SON decreased significantly to 8.5% of the control 15 min post-scald (P<0.01), and gradually recovered to 31.5% and 52.4% of the control 60 min and 180 min post-scald respectively, though still significantly lower than the control (P<0.01). Pre- and post-scald ET-1 gene transcription in rat hypothalamus was also measured by Northern blot hybridization. No significant difference in the quantity of ET-1 mRNA was found between 15 min post-scald data and those of the control. The quantity increased to a significantly higher level 60 min post-scald (P<0.05) and further increased to 2.5 creased to 2.5 fold of the control 180 min post-scald (P<0.05). In addition, the Northern blot hybridization showed that the post-scald size of ET-1 mRNA remained unchanged despite of the increase in quantity. In view of the neuroendocrine role of SON, the changes in ET-1 mRNA and ET-1-ir in SON resulting from scald suggest that ET-1 may play an important role in neuroendocrine reactions following scald.
Jiang
Y M
YM
Department of Physiology, Second Military Medical University, Shanghai 200433, China.
Yuan
W J
WJ
Xiang
Z H
ZH
Miao
W M
WM
Lin
L
L
Li
L
L
Jiao
B H
BH
D000818:Animals; D002056:Burns / Q000235:genetics* / Q000378:metabolism; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013495:Supraoptic Nucleus / Q000378:metabolism*; D014158:Transcription, Genetic
D019332:Endothelin-1; D012333:RNA, Messenger