title: The pig platelet-activating factor receptor gene is expressed at the mRNA level in different tissues and is mapped to chromosome 6.
abstract: After the pig platelet-activating factor receptor (PAFr) gene was cloned and sequenced, the chromosomal location of this gene was studied using a pig/rodent somatic cell hybrid panel containing 27 cell lines. The results indicated that the pig PAFr gene is located on SSC6q22-23. Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is thought to be very important in the animal reproductive processes. Its function is mediated through a membrane-bound receptor. Pig PAFr mRNA distribution in different tissues was tested using reverse transcription and PCR (RT-PCR) reactions. All tissues examined expressed PAFr. Using a pig PAFr gene DNA competitor, PAFr expression was quantificated. The pig PAFr mRNA expression level was estimated to be from 1 x 10(2) to 1.2 x 10(4) copies of complementary DNA (cDNA) per 50 ng of total RNA. The highest level was found in lung, and the lowest in the skeletal muscle. These results demonstrated that PAFr was differentially expressed in pig tissues.
authors.affiliation: Department of Animal and Veterinary Sciences, Clemson University, SC 29634, USA.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Yang
authors.forename: John R
authors.initials: JR
authors.lastname: Diehl
authors.forename: Laura
authors.initials: L
authors.lastname: Grapes
authors.forename: Max F
authors.initials: MF
authors.lastname: Rothschild
authors.forename: William E
authors.initials: WE
authors.lastname: Roudebush
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004717:Endometrium / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D007668:Kidney / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D018482:Muscle, Skeletal / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry; D009928:Organ Specificity; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*
chemical_list: D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor