[Construction of three transgenic vectors carrying the latent membrane protein gene of Epstein-Barr virus].
In order to produce transgenic mice carrying the latent membrane protein(LMP) gene of Epstein-Barr virus(EBV) and to study the oncogenic role of LMP gene in vivo, three different transgenic vectors carrying the LMP gene were constructed. In pBR322-MT-LMP vector, the promoter of LMP gene is the regulation region of mouse metallothionein-I gene. In pMci3-LMP shuttle vector, the promoter of LMP gene is the replication origin(oriP) of EBV. In pMV-LMP-c-myc retroviral vector, the long terminal repeat (LTR) of mouse sarcomavirus serves as the promoter of LMP gene. The characterization and usefulness of these three transgenic vectors are also discussed.
Xiao
Z
Z
Cancer Research Institute, Hunan Medical University, Changsha 410078.
Guan
Y
Y
Duan
C
C
Chen
Z
Z
Yao
K
K
D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D012190:Retroviridae / Q000235:genetics; D020079:Terminal Repeat Sequences; D019076:Transgenes; D014763:Viral Matrix Proteins / Q000235:genetics*
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D008668:Metallothionein