[Effect of SP on intracellular free calcium concentration in cultured osteoblast].
OBJECTIVE
To investigate the effect of SP on intracellular free calcium concentration (Ca2+)i in cultured osteoblast.


METHODS
Effect of SP on intracellular free calcium was measured using cell culturing and laser scanning confocal microscopy with fluo-3/AM as a fluorescence probe.


RESULTS
After treated with SP(10(-4) mol/L), 70% of the cells responded to it; the intracellular (Ca2+)i increased markedly [fluorescence intensity increased by (90 +/- 18)%].


CONCLUSIONS
These results suggested that calcium may have a role as a intracellular secondary messenger in the action of SP in osteoblast.
Sun
Y
Y
Department of Orthodontics, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Duan
Y
Y
Fan
C
C
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002478:Cells, Cultured; D010006:Osteoblasts / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015290:Second Messenger Systems; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P; D002118:Calcium