Identification of differentially expressed genes in human uterine leiomyomas using differential display.
In searching of differentially expressed genes in human uterine leiomyomas, differential display was used with twelve pairs of primers to compare human uterine leiomyomas with matched myometrium. False positives were eliminated by reverse Northern analysis. Positives were confirmed by Northern blot analysis.


RESULTS
Four of 69 cDNA fragments (3 up-regulated named L1, L2 and L3 and 1 down-regulated named M1 in leiomyoma) were confirmed by Northern analysis. Sequence comparison and Northern analysis proved that L1 is exactly the human ribosomal protein S19. It was present ubiquitously in 13 tissues tested but in various levels and even in different size. L1 was highly expressed in parotidean cystadenocarcinoma, pancreatic cancer and breast cancer examined. No mutations have been found in human uterine leiomyomas (n=6).


CONCLUSIONS
hRPS19 overexpression might be a universal signal in rapid cell growth tissues.
Li
Bin
B
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences.
Sun
Mei
M
He
Bin
B
Yu
Jin
J
Zhang
You Duan
YD
Zhang
Yong Lian
YL
D018076:DNA, Complementary / Q000032:analysis / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007889:Leiomyoma / Q000235:genetics / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D012269:Ribosomal Proteins / Q000378:metabolism; D014018:Tissue Distribution; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D019151:Peptide Library; D012269:Ribosomal Proteins; C040343:ribosomal protein S19