Selective binding of mannose-encapsulated gold nanoparticles to type 1 pili in Escherichia coli.
The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.
Lin
Chun-Cheng
CC
Institute of Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan.
Yeh
Yi-Chun
YC
Yang
Chan-Yi
CY
Chen
Chan-Long
CL
Chen
Gee-Fong
GF
Chen
Chia-Chun
CC
Wu
Yi-Chun
YC
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000378:metabolism; D018830:Adhesins, Escherichia coli; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D006046:Gold; D008358:Mannose