[Studies on the preparation and characters of anti-AFB1 antibody from the yolk of laying hens].
AFB1O-BSA conjugates were injected into four laying hens (A, B, C and D). The yolk anti-AFB1 antibodies A,C and D produced were obviously increased after 90 days of the first injection, and reached the peak after 135 days, and started dropping after 165 days. The indirect ELISA titer of yolk antibodies A,C and D were 1:8000, 1:6000 and 1:6000 respectively. The specificity of the antibody A was good, but a cross-reaction with AFB2, AFG1, AFG2, with an exception of AFM1, was found. The sensitivities of AFB1, AFB2, AFG1 and AFG2 were 6, 25, 125 and 2495 micrograms/L respectively.
Chen
F
F
Agro-Microbiology Key Laboratories Huazhong Agricultural University, Wuhan 430070, China.
Li
G
G
Chen
J
J
Luo
X
X
Zhou
Q
Q
D016604:Aflatoxin B1 / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002645:Chickens; D003429:Cross Reactions; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D012680:Sensitivity and Specificity
D000906:Antibodies; D016604:Aflatoxin B1