Tools for the study of genome rearrangements in laboratory and industrial yeast strains.
In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations.
Lockhart
Lesley
L
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Oliver
Stephen G
SG
Delneri
Daniela
D
D015139:Blotting, Southern; D002869:Chromosome Aberrations; D015342:DNA Probes / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D019143:Evolution, Molecular; D016681:Genome, Fungal; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal