Binding of 5-bromouracil-containing S/MAR DNA to the nuclear matrix.
Substitution of thymine with 5-bromouracil in DNA is known to change interaction between DNA and proteins, thereby inducing various biological phenomena. We hypothesize that A/T-rich scaffold/nuclear matrix attachment region (S/MAR) sequences are involved in the effects of 5-bromodeoxyuridine. We examined an interaction between DNA containing an intronic S/MAR sequence of the immunoglobulin heavy chain gene and nuclear halos prepared from HeLa cells. Upon substitution with 5-bromouracil, the S/MAR DNA bound more tightly to the nuclear halos. The multi-functional nuclear matrix protein YY1 was also found to bind more strongly to 5-bromouracil-substituted DNA containing its recognition motif. These results are consistent with the above hypothesis.
Ogino
Hideki
H
Kihara Institute for Biological Research and Graduate School of Integrated Science, Yokohama City University, Japan.
Fujii
Michihiko
M
Satou
Wataru
W
Suzuki
Toshikazu
T
Michishita
Eriko
E
Ayusawa
Dai
D
D001976:Bromouracil / Q000737:chemistry / Q000378:metabolism*; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D050981:Erythroid-Specific DNA-Binding Factors; D006367:HeLa Cells; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D013941:Thymine / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D050986:YY1 Transcription Factor
D050981:Erythroid-Specific DNA-Binding Factors; D007143:Immunoglobulin Heavy Chains; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D001976:Bromouracil; D004247:DNA; D013941:Thymine