title: Specific localization of an inwardly rectifying K(+) channel, Kir4.1, at the apical membrane of rat gastric parietal cells; its possible involvement in K(+) recycling for the H(+)-K(+)-pump.
abstract: Hydrochloric acid (HCl) is produced in parietal cells of gastric epithelium by a H(+)-K(+) pump. Protons are secreted into the gastric lumen in exchange for K(+) by the action of the H(+)-K(+)-ATPase. Luminal K(+) is essential for the operation of the pump and is thought to be supplied by unidentified K(+) channels localized at the apical membrane of parietal cells. In this study, we showed that histamine- and carbachol-induced acid secretion from isolated parietal cells monitored by intracellular accumulation of aminopyrine was depressed by Ba(2+), an inhibitor of inwardly rectifying K(+) channels. Among members of the inwardly rectifying K(+) channel family, we found with reverse transcriptase-polymerase chain reaction analyses that Kir4.1, Kir4.2 and Kir7.1 were expressed in rat gastric mucosa. With immunohistochemical analyses, Kir4.1 was found to be expressed in gastric parietal cells and localized specifically at their apical membrane. The current flowing through Kir4.1 channel expressed in HEK293T cells was not affected by reduction of extracellular pH from 7.4 to 3. These results suggest that Kir4.1 may be involved in the K(+) recycling pathway in the apical membrane which is required for activation of the H(+)-K(+) pump in gastric parietal cells.
authors.affiliation: Department of Pharmacology II, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Akikazu
authors.initials: A
authors.lastname: Fujita
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Horio
authors.forename: Kayoko
authors.initials: K
authors.lastname: Higashi
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Mouri
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Hata
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeguchi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kurachi
mesh_terms: D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D016764:Cell Polarity / Q000502:physiology; D005110:Extracellular Space / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016253:Microscopy, Immunoelectron; D010295:Parietal Cells, Gastric / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism; D026902:Potassium Channel Blockers / Q000494:pharmacology; D024661:Potassium Channels, Inwardly Rectifying / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000631:Aminopyridines; D026902:Potassium Channel Blockers; D024661:Potassium Channels, Inwardly Rectifying; D017506:H(+)-K(+)-Exchanging ATPase; D011188:Potassium