Artificial inseminations with frozen semen and application of DNA fingerprint for parents/offspring conformation on Great Pyrenees.
Frozen semen of Great Pyrenees, air-shipped from France, was artificially inseminated (AI) to two Great Pyrenees bitches. Blood LH and P concentrations and vulval width principally determined optimal breeding time of bitches. AI were performed on 17 to 21 days from the onset of estrous bleeding, and 25 days after final Al, pregnancy of one bitch, with two fetuses, was identified by ultrasound image diagnostic equipment. On 62 days, the bitch whelped one male pup and one female pup. Blood samples were obtained from four parents and offspring, and their DNA was extracted. DNA samples digested with four kinds of restriction endonucleases, and resulting fragmented DNA were used as test samples for DNA fingerprinting with Myo prove. As a result bands from pups, displayed by any enzyme, were inherited from either parent, and clear parent-offspring relationships were identified.
Takagi
K
K
Department of Veterinary Obstetrics and Gynecology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. takagi@brs.nihon-u.ac.jp
Tsumagari
S
S
Ichikawa
Y
Y
Yukawa
M
M
Takeishi
M
M
D000818:Animals; D001947:Breeding; D015925:Cryopreservation / Q000662:veterinary; D016172:DNA Fingerprinting / Q000662:veterinary*; D004285:Dogs / Q000235:genetics* / Q000502:physiology; D004971:Estrus; D005260:Female; D005306:Fertilization; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D010375:Pedigree; D011247:Pregnancy; D012662:Semen Preservation / Q000662:veterinary; D013997:Time Factors