Efficient method for expressing transgenes in nonhuman primate embryos using a stable episomal vector.
Transgenesis in the nonhuman primate can enhance the study of human biology by providing animal models for the study of primate-specific physiology, pathophysiology, and embryonic development. Progress with this technology has been hindered by the inherent inefficiency of transgenesis, transgene silencing, and practical restrictions on the production of sufficient pronuclear stage nonhuman primate zygotes. We have developed a novel technique using an Epstein Barr virus (EBV)-based episomal vector to produce rhesus monkey (Macaca mulatta) embryos expressing a transgene. Plasmid DNA containing the latent origin of replication, oriP, and Epstein Barr Nuclear Antigen-1 (EBNA-1) of EBV, as well as a CMV IE-enhanced green fluorescent protein (eGFP) expression cassette, was introduced into rhesus embryos by direct pronuclear microinjection. We detected eGFP in early cleavage stage embryos (4-8 cell) and throughout the duration of culture (day 8-9 blastocysts) by epifluorescent microscopy. A 50% transduction rate was obtained with the EBV-based vector. Microinjected embryos expressed eGFP and retained their developmental capacity as evidenced by development to the blastocyst stage. EBV-based vectors present a novel and efficient means of delivering transgenes for the study of the molecular control of primate embryonic development.
Wolfgang
Michael J
MJ
Wisconsin Regional Primate Research Center, University of Wisconsin, 1223 Capitol Court, Madison, WI 53715-1299, USA.
Marshall
Vivienne S
VS
Eisele
Stephen G
SG
Schotzko
Michele L
ML
Thomson
James A
JA
Golos
Thaddeus G
TG
D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008253:Macaca mulatta / Q000196:embryology*; D010957:Plasmids; D019076:Transgenes
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins