Binding of bovine lactoferrin to Streptococcus dysgalactiae subsp. dysgalactiae isolated from cows with mastitis.
Three strains of Streptococcus dysgalactiae subsp. dysgalactiae (UT516, UT519, ATCC 27957) were used to determine if bovine lactoferrin (Lf) binds to bacterial cells by biotin avidin binding assay (BABA), enzyme-linked immunosorbent assay (ELISA), and binding inhibition assay. Binding assays revealed that all strains of S. dysgalactiae subsp. dysgalactiae (S. dysgalactiae) evaluated in this study bound to Lf. However, differences in Lf binding capability among strains and between methods used were detected. Binding of Lf was not inhibited by transferrin (Tf) and Lf moiety molecules (mannose, galactose, and lactose) but by Lf. This study demonstrates that S. dysgalactiae bound to bovine Lf in a specific manner.
Park
Hee Myung
HM
Food Safety Center of Excellence, The University of Tennessee, Knoxville, TN 37996, USA.
Almeida
R A
RA
Luther
D A
DA
Oliver
S P
SP
D000818:Animals; D001360:Avidin / Q000378:metabolism; D001667:Binding, Competitive; D001681:Biological Assay / Q000662:veterinary; D001710:Biotin / Q000378:metabolism; D019921:Biotinylation; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007781:Lactoferrin / Q000378:metabolism*; D008414:Mastitis, Bovine / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000302:isolation & purification / Q000378:metabolism*
D001360:Avidin; D001710:Biotin; D007781:Lactoferrin