A filtration, incubation and staining reactor including a new protocol for FISH.
A newly developed device for performing fluorescence in situ hybridization (FISH) is described. An adapted procedure was compared with two typical FISH protocols. Tests were performed with Pseudomonas cells and the gene probe EUB338. With the novel procedure, we obtained a better recovery of cells and less variability in results.
Poschen
Lothar
L
Forschungszentrum Jülich, Institut für Chemie und Dynamik der Geosphäre (ICG), Institut IV: Agrosphäre, Germany. l.poschen@fz-juelich.de
Klauth
Peter
P
Groeneweg
Joost
J
Wilhelm
Ralf
R
D016477:Artifacts; D005374:Filtration / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes; D011549:Pseudomonas / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods
D015335:Molecular Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S