[Study of rat osteoblasts transfected by transforming growth factor-beta 1 gene].
OBJECTIVE
To investigate the effect of transforming growth factor-beta 1 (TGF-beta 1) gene transfer on the biological characteristics of osteoblasts.


METHODS
The expression of TGF-beta 1 in the transfected osteoblasts was detected by in situ hybridization and assay of TGF-beta 1 activity in the supernatant (mink lung epithelium cell growth-inhibition test). The effects of gene transfer and supernatant of the transfected osteoblasts on the proliferation and alkaline phosphatase(ALP) activity of osteoblasts were detected by 3H-TdR and MTT.


RESULTS
The results of in situ hybridization analysis suggested that the osteoblasts transfected by TGF-beta 1 gene could express TGF-beta 1 obviously. The complex medium, which was the mixture of serum-free DMEM and the activated supernatant according to 1:1, 1:2, 1:4, could inhibit growth of Mv-1-Lu evidently and the ratios of inhibition were 16.3%, 22.7%, 28.2% respectively. TGF-beta 1 gene transfer had no effect on the biological characteristics of osteoblasts, but the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts.


CONCLUSIONS
TGF-beta 1 gene transfer promotes the expression of TGF-beta 1 and the biological characteristics of transfected osteoblasts are stable, which is helpful for gene therapy of bone defects in vivo.
Liu
Yong
Y
Department of Orthopedic Surgery, Xiehe Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China 430022.
Du
Jing-Yuan
JY
Zheng
Qi-Xin
QX
Wang
Hong
H
Guo
Xiao-dong
XD
Duan
De-Yu
DY
D000818:Animals; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*; D053773:Transforming Growth Factor beta1
C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1