title: Comprehensive karyotyping of the HT-29 colon adenocarcinoma cell line.
abstract: The tumor cell line HT-29 was derived from a primary adenocarcinoma of the rectosigmoid colon. HT-29 is hypertriploid (3n+) and has accumulated numerous chromosomal structural aberrations. To identify material involved in chromosome rearrangements, we performed a comprehensive cytogenetic analysis using G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH). The combination of molecular cytogenetic techniques enabled us to define the first comprehensive karyotype for HT-29. Seventeen marker chromosomes were found in 75-100% of metaphase cells, generally in a single copy per cell. We confirmed the composition of eight previously described markers, refined the classification of seven others, and identified two novel marker chromosomes. Notable aberrations included a reciprocal translocation between chromosomes 6 and 14 and an unusual, large derivative chromosome 8 composed entirely of 8q material. The telomere status, evaluated by FISH, revealed telomeric signals at the termini of all chromosomes. No interstitial telomeric sequences were observed in any cell. Although numerous chromosomal aberrations are present in HT-29, the cell line appears to have retained a high level of genomic stability during passage in culture since undergoing transformation. The excellent resolving power of SKY, coupled with additional information obtained from molecular cytogenetic analyses, will improve our ability to identify genetic lesions characteristic of cancer.
authors.affiliation: UCSD Cancer Center and Department of Pathology, University of California San Diego, La Jolla, California 92093-0912, USA.
authors.forename: Kanji
authors.initials: K
authors.lastname: Kawai
authors.forename: Carrie
authors.initials: C
authors.lastname: Viars
authors.forename: Karen
authors.initials: K
authors.lastname: Arden
authors.forename: David
authors.initials: D
authors.lastname: Tarin
authors.forename: Virginia
authors.initials: V
authors.lastname: Urquidi
authors.forename: Steve
authors.initials: S
authors.lastname: Goodison
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D019073:HT29 Cells / Q000166:cytology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods; D016615:Telomere / Q000235:genetics
chemical_list: D005819:Genetic Markers