[Effect of xiongshao capsule on proliferation and apoptosis of vascular smooth muscle cells in rabbits observed by serum pharmacological method].
OBJECTIVE
To observe the effect of Xiongshao Capsule (XSC) on proliferation and apoptosis of aortic smooth muscle cells (SMC) of rabbits by serum pharmacological method.


METHODS
SMC were isolated from thoracic aorta of the normal rabbits and passaged. The SMC proliferation model was established by endothelin (ET). The medicated serum was prepared after given the normal rabbits suspension of XSC through gastrogavage for 10 days. The proliferation and apoptosis of SMC were observed by methyl thiazolyl tetrazolium (MTT) colorimetry, flow cytometry and agarose gel electrophoresis.


RESULTS
ET obviously promoted proliferation of SMC, and the serum medicated with XSC could lower the proportion of SMC in S + G2 phase and markedly inhibited the proliferation of SMC caused by ET in dose dependent manner. It also had certain effect on inducing apoptosis of SMC.


CONCLUSIONS
XSC could markedly inhibit the proliferation of SMC caused by ET and induce apoptosis of SMC, which might be the mechanism of preventing coronary restenosis after interventional treatment.
Xu
H
H
Department of Cardiovascular Diseases, Xiyuan Hospital, China Academy of TCM, Beijing 100091.
Shi
D
D
Chen
K
K
D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology; D017209:Apoptosis / Q000187:drug effects*; D002214:Capsules; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016232:Endothelins / Q000037:antagonists & inhibitors; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011817:Rabbits
D002214:Capsules; D004365:Drugs, Chinese Herbal; D016232:Endothelins