[Bsu50441--isoschizomer of endonuclease restriction AsuI].
Endonuclease of restriction of type II has been found in Bacillus subtilis B-5044 during testing of endophytic strains of cotton plants bacilli. The restrictase Bsu5044I hydrolysed DNA of M13mp18 phage in 4 sites; pUC19 DNA in 5 sites; pBR322 in 15 sites. There were a lot of sites restriction in DNAs of phages T7 and lambda. A comparison of electrophoretical divisions of Bsu5044I and Cfr13I fragments of phages and plasmid DNAs showed their identity. Thus, the found restrictase Bsu5044I is an isoschisomer of AsuI.
Reva
O M
OM
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154, Zabolotny St., Kyiv, 03143, Ukraine.
Luk'ianchuk
V V
VV
Polishchuk
L V
LV
D001412:Bacillus subtilis / Q000201:enzymology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis*; D003368:Gossypium / Q000382:microbiology*
D015252:Deoxyribonucleases, Type II Site-Specific; C032262:GGNCC-specific type II deoxyribonucleases
Bsu50441--izoshyzomer endonukleazy restryktsiï AsuI.