Changes in UCP2, PPARgamma2, and c/EBPalpha gene expression induced by a neuropeptide Y (NPY) related receptor antagonist in overweight rats.
Neuropeptide Y (NPY), a peptide released by nervous cells, appears to contribute to adiposity regulation by increasing food intake and inhibiting lipolysis. New NPY receptor related antagonists such as S.A.0204 are being developed as potential anti-obesity drugs affecting adipocyte lipid metabolism and thermogenesis. In this sense, those animals fed on a high-energy yielding (cafeteria) diet decreased body fat weight as compared to overweight controls, when they were administered with S.A.0204, and increased body temperature, which statistically correlated with high UCP2 mRNA expression levels in white adipose tissue. In addition, the in vivo NPY-antagonist administration was able to prevent white adipose tissue growth in animals fed the cafeteria (high-fat) diet by impairing PPARy and CIEBPalpha mRNA expression in white fat cells. In summary, this novel NPY related-antagonist S.A.0204 may regulate body fat deposition by affecting both energy dissipation and white adipose tissue deposition, representing a potential new pharmacological strategy for obesity management.
Margareto
J
J
Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
Rivero
I
I
Monge
A
A
Aldana
I
I
Marti
A
A
Martínez
J A
JA
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D001831:Body Temperature; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics*; D004041:Dietary Fats / Q000008:administration & dosage; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D017476:Receptors, Neuropeptide Y / Q000037:antagonists & inhibitors*; D022722:Thermogenesis / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics*; D000071246:Uncoupling Protein 2
D022763:CCAAT-Enhancer-Binding Protein-alpha; D004041:Dietary Fats; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D017476:Receptors, Neuropeptide Y; D014157:Transcription Factors; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2