TGF-beta1-conditioned glial cell-derived dendritic cells inhibit expansion of MBP-reactive T cells in vitro.
Resident microglial cells contribute to activation and expansion of T cells under inflammatory conditions within the CNS. However, there is no evidence how interactions between microglia and T cells affect CNS inflammation. We evaluated the effect of glial cell-derived dendritic cells (GC-DC) in expanding and eliminating myelin basic protein (MBP)-reactive T cells. GC-DC untreated with TGF-beta1 (GC-DC0) primed antigen specific T cell proliferation, whereas GC-DC treated with TGF-1 (GC-DCbeta) effectively inhibited expansion of T cells via inducing IFN-y-expressing CD8+ T cells. Augmented IFN-gamma and/orTNF-alpha might also affect the elimination of MBP-reactive T cells. These results indicate that TGF-beta1-mediated functional skewing of GC-DC plays a critical role for the elimination of MBP-reactive T cells.
Xu
Ling-Yun
LY
Division of Neurology, Huddinge University Hospital, Karolinska Institute, Sweden.
Yang
Jian-She
JS
Xiao
Bao-Guo
BG
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1
D004676:Myelin Basic Protein; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016178:Granulocyte-Macrophage Colony-Stimulating Factor