Rapid detection of avian pneumovirus in tissue culture by microindirect immunofluorescence test.
An indirect immunofluorescence (IFA) test with a 96-well, flat-bottomed microplate was developed to detect avian pneumovirus (APV) antigen in Vero cell cultures. Samples of nasal turbinates and swabs from infraorbital sinuses and trachea were collected from 4-week-old poults experimentally inoculated with APV. The APV titers by tissue culture IFA staining were compared with that of visual reading of cytopathic effect (CPE). The ability of IFA staining to detect APV antigen correlated well with visualizing CPE. The use of IFA staining of Vero cell cultures allowed detection of APV in substantially less time than the use of visualizing CPE. In addition, the use of IFA allowed specific identification of the virus in cell culture.
Jirjis
Faris F
FF
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Noll
Sally L
SL
Halvorson
David A
DA
Nagaraja
Kakambi V
KV
Townsend
Evelyn L
EL
Goyal
Sagar M
SM
Shaw
Daniel P
DP
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary*; D018111:Pneumovirus / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D018186:Pneumovirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000276:immunology; D014422:Turkeys; D014709:Vero Cells
D000956:Antigens, Viral