title: Characterization of the mouse protoporphyrinogen oxidase gene.
abstract: The murine protoporphyrinogen oxidase gene has been isolated, characterized and localized. The gene spans 4.2 kb, is comprised of 13 exons and 12 introns, and is located on chromosome 1 in band 1 H2. Analysis of 1.2 kb of the 5' upstream region revealed a promoter which is not GC rich and lacks any TATA boxes or initiator elements in the vicinity of the transcription start site. A variety of putative transcriptional element binding sequences were identified and gel shift assays support the presence of two GATA-1 sites near -760 bp as well as AP-1, AP-2, and Sp1 sites in the -1200 bp 5' flanking region. Luciferase reporter constructs transiently expressed in erythroid cell lines demonstrated erythroid-specific expression with the -1160 bp, but not with the -746 bp or -198 bp constructs. Expression in nonerythroid cells occurred maximally with -1160 bp, but was significant with -746 bp and absent with -198 bp. Expression of both housekeeping and erythroid-specific fusions in the transient expression systems was greatly decreased in the -5000 bp constructs suggesting the presence of repressor elements in the -1160 to -5000 bp region.
authors.affiliation: The Biomedical and Health Sciences Institute, Department of Microbiology, University of Georgia, Athens 30602-7229, USA. hdailey@arches.uga.edu
authors.forename: Tamara A
authors.initials: TA
authors.lastname: Dailey
authors.forename: Julie F
authors.initials: JF
authors.lastname: McManus
authors.forename: Harry A
authors.initials: HA
authors.lastname: Dailey
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000378:metabolism; D005091:Exons; D005420:Flavoproteins; D015870:Gene Expression; D020043:Genes, Essential / Q000235:genetics*; D007438:Introns; D051379:Mice / Q000235:genetics*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D050768:Protoporphyrinogen Oxidase; D020218:Response Elements / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D005420:Flavoproteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C493979:Ppox protein, mouse; D050768:Protoporphyrinogen Oxidase