title: Functional characterization of the Candida albicans homologue of secretion-associated and Ras-related (Sar1) protein.
abstract: Secretion-associated and Ras-related protein (Sar1p) plays an essential role during the protein transport from the endoplasmic reticulum to the Golgi apparatus. The cDNA sequence of the Sar1 gene has been identified and characterized from the human yeast pathogen, Candida albicans. This cDNA encodes a protein of 190 amino acids, which shares a 78% sequence identity with Saccharomyces cerevisiae Sar1p and contains the conserved GTP-binding motifs of the small GTPase superfamily. Complementation studies confirmed that this cDNA encodes the functional homologue of ScSar1p. The recombinant C. albicans Sar1p exhibits GTP-binding activity in vitro that was abolished by deletion of one of the three GTP-binding motifs.
authors.affiliation: Mammalian Cell Genetics, Pharmaceutical Biotechnology Sector, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R2.
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
authors.forename: Chang-Muk
authors.initials: CM
authors.lastname: Lee
authors.forename: Shi-Hsiang
authors.initials: SH
authors.lastname: Shen
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002176:Candida albicans / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology; D012331:RNA, Fungal / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D033921:Vesicular Transport Proteins
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D012331:RNA, Fungal; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D020559:Monomeric GTP-Binding Proteins; C077395:SAR1 protein, S cerevisiae