title: Improved cell growth in tobacco suspension cultures expressing Vitreoscilla hemoglobin.
abstract: Expression of the gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been previously used to improve recombinant cell growth and enhance product formation under microaerobic conditions, a common phenomenon in large-scale cultivations of bacteria. This technology has now been applied to tobacco suspension cultures. Tobacco suspension cultures have been generated from VHb-expressing tobacco plants. Cell cultures were capable of producing an active hemoglobin. When grown in shake flasks, the cells did not show any lag-phase and exhibited improved cell growth, compared to controls carrying the parental plasmid.
authors.affiliation: Institute of Biotechnology, ETH-Zürich, CH-8093, Switzerland.
authors.forename: Judith
authors.initials: J
authors.lastname: Farrés
authors.forename: Pauli T
authors.initials: PT
authors.lastname: Kallio
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D046508:Culture Techniques; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005240:Feasibility Studies; D005285:Fermentation; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism; D018527:Hydroponics; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D054793:Truncated Hemoglobins
chemical_list: D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D000431:Ethanol; D013395:Sucrose