[Proteomics in clinical research: new approach of mass spectrometry].
A proteome has been defined as the protein complement expressed by the genome of an organism, tissue, or differentiated cell. Knowledge of complete genome sequences has led to considerable effort being increasingly devoted to the large-scale study of proteomes, that is, 'proteomics'. Commonly, two proteomes are compared by a substructive analysis in which differences due to drug treatment, culture conditions, genetic variations, or diseases can be observed. Two-dimensional gel electrophoresis and mass spectrometry are commonly used for this purpose. We applied this approach to the analysis of vitreous humor(VH) proteins. Fifty-two different proteins were identified on silver-stained 2D-gel patterns with VH proteins obtained from diabetic retinopathy and macular hole. Thirty-five proteins, which have not reported in plasma, were found in VH. Pigment epithelium-derived factor, which was reported to be a potent inhibitor of angiogenesis in cornea and vitreous was at a higher concentration in VH with diabetes than in that with macular hole. It is impressive that the inhibitor increases in the vitreous with proliferative angiogenesis. Unique applications in proteomics promise a bright future for molecular biology and hopefully for clinical chemistry.
Shimizu
Akira
A
Department of Clinical Pathology, Osaka Medical College, Takatsuki 569-8686.
Nakanishi
Toyohumi
T
Koyama
Reiko
R
Ikeda
Tsunehiko
T
D001082:Aqueous Humor / Q000737:chemistry; D016510:Corneal Neovascularization; D003930:Diabetic Retinopathy / Q000209:etiology; D015180:Electrophoresis, Gel, Two-Dimensional; D005136:Eye Proteins / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry; D009414:Nerve Growth Factors; D011506:Proteins / Q000302:isolation & purification; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification; D015843:Serpins / Q000302:isolation & purification; D000099174:Pigment Epithelium-Derived Factor
D005136:Eye Proteins; D009414:Nerve Growth Factors; D011506:Proteins; D020543:Proteome; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor