EPR spectroscopy of common nitric oxide - spin trap complexes.
Two commonly used hydrophobic and hydrophilic spin traps for NO, namely Fe2+(DETC)(2)and Fe2+(MGD)(2), respectively, were analyzed via EPR spectroscopy. EPR spectra of trapped NO, together with field position standards, were recorded both in the frozen state and at room temperature. We present a detailed characterization of the EPR spectra of the above paramagnetic NO complexes, concerning g-value, hyperfine splitting and linewidths. This study also provides spectroscopic data required to develop a quantitative and sensitive detection system for nitric oxide both in hydrophobic and hydrophilic aqueous media.
Nedeianu
Saviana
S
Institute of Biophysics, Biological Research Centre Szeged, P.O. Box 521, 6701 Szeged, Hungary.
Páli
Tibor
T
D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D009569:Nitric Oxide / Q000737:chemistry*; D019097:Spin Trapping / Q000379:methods*
D009569:Nitric Oxide