The molecular characterization of maize B chromosome specific AFLPs.
The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGG
Qi
Zhong Xia
ZX
The College of Life Sciences, Nankai University, Tianjin, China.
Zeng
Hui
H
Li
Xiu Lan
XL
Chen
Cheng Bin
CB
Song
Wen Qin
WQ
Chen
Rui Yang
RY
D001483:Base Sequence; D002503:Centromere / Q000737:chemistry; D032461:Chromosomes, Plant / Q000737:chemistry*; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D016615:Telomere / Q000737:chemistry; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers