title: Slow axonal transport of the cytosolic chaperonin CCT with Hsc73 and actin in motor neurons.
abstract: Molecular chaperones are well known for their role in facilitating the folding of nascent and newly synthesized proteins, but have other roles, including the assembly, translocation and renaturation of intracellular proteins. Axons are convenient tissues for the study of some of these other roles because they lack the capacity for significant protein synthesis. We examine the axonal transport of the cytosolic chaperonin containing T- complex polypeptide 1 (CCT) by labeling lumbar motor neurons with [35S]methionine and examining sciatic nerve proteins by 2-D gel electrophoresis and immunoblotting. All CCT subunits identifiable with specific antibodies, namely CCTalpha, CCTbeta, CCTgamma and CCTepsilon/CCTtheta; (the latter two subunits colocalized in analyses of rat nerve samples), appeared to be labeled in "slow component b" of axonal transport along with the molecular chaperone Hsc73 and actin, a major folding substrate for CCT. Our results are consistent with molecular chaperones having a post-translational role in maintaining the native form of actin during its slow transport to the axon terminal and ensuring its correct assembly into microfilaments.
authors.affiliation: Department of Physiology and The Neuroscience Center, School of Medical Sciences, University of Otago, Dunedin, New Zealand. bougr173@student.otago.ac.nr
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Bourke
authors.forename: Wathik
authors.initials: W
authors.lastname: El Alami
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Wilson
authors.forename: Aidong
authors.initials: A
authors.lastname: Yuan
authors.forename: Anne
authors.initials: A
authors.lastname: Roobol
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Carden
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D015151:Immunoblotting; D018832:Molecular Chaperones / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve
chemical_list: D000199:Actins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C494373:Hspa8 protein, rat; D018832:Molecular Chaperones; C533316:Tcp1 protein, rat; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins