Molecular characterization of breast cancer cell lines by expression profiling.
OBJECTIVE
Gene expression patterns provide detailed insights into cellular regulation that reflect minor differences of cellular capacity not accessible by standard descriptions of the cellular phenotype or origin.


METHODS
To identify fundamental differences and similarities we analyzed the gene expression patterns of four breast cancer cell lines: MCF-7, SK-BR-3, T-47D, and BT-474.


RESULTS
Although only a small subset of genes (597) is represented on the Atlas-cDNA-Array (Clontech) used, clear differences in the expression of a number of genes could be detected. For example, unique high levels of expressions were found for the HLH-protein ID-1 (MCF-7) and the receptor tyrosine kinase erbB2 (SK-BR-3 and T-47D). Most genes analyzed were expressed at comparable levels in all cell lines studied.


CONCLUSIONS
For interpretation of the results sets of genes that show similar variation of expression among the cells were grouped together. Furthermore, our analysis allows the assignment of similarity values that lead to a relation profile of the cell lines. How these results correlate with known biological properties of the cell lines is discussed. Additionally, we demonstrate that results obtained by cDNA-Array hybridization for expression of the ErbB receptor family correlate well with competitive RT-PCR, thus confirming the reliability of the cDNA-Array analysis.
Kroll
T
T
Clinic for Internal Medicine II (Hematology, Oncology, Metabolic Diseases and Diabetes), Friedrich-Schiller-University Jena, Erlanger Allee 101, 07740 Jena, Germany.
Odyvanova
L
L
Clement
J H
JH
Platzer
C
C
Naumann
A
A
Marr
N
N
Höffken
K
K
Wölfl
S
S
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D066246:ErbB Receptors / Q000096:biosynthesis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1; D020411:Oligonucleotide Array Sequence Analysis; D066247:Receptor, ErbB-4; D012097:Repressor Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D012097:Repressor Proteins; D014157:Transcription Factors; C587689:ERBB4 protein, human; D066246:ErbB Receptors; D066247:Receptor, ErbB-4