[Detection of HPV type 16, 18 infection and p53 protein overexpression in oral squamous cell carcinoma].
OBJECTIVE
To study the action and interaction of HPV16, 18 infection and p53 protein overexpression in carcinogenesis of oral squamous cell carcinoma (OSCC).


METHODS
Polymerase chain reaction (PCR) was used for detection of HPV16, 18 DNA, immunohistochemical technique (LSAB) for detection of p53 protein overexpression.


RESULTS
In HPV-DNA analysis, HPV 16, 18 DNA were demonstrated significantly (P < 0.05) more often (48.89%, 22 of 45; 15 cases positive with HPV16-DNA and 7 cases positive with HPV18-DNA) in OSCC than in normal oral mucosa (negative). The frequency of p53 protein overexpression was 62.22% (28 of 45) in OSCC, while negative in normal oral mucosa (P < 0.01). The average survival time and survival rate of patients with HPV16, 18 infection and p53 overexpression was shorter than that of patients without HPV infection (P > 0.05) and without p53 overexpression (P < 0.05).


CONCLUSIONS
The result suggests that HPV16, 18 infection plays a significant role in carcinogenesis of OSCC. p53 mutation is a frequent genetic event in OSCC. HPV status and p53 protein overexpression partially affect the average survival time and survival rate of OSCC patients.
Minawaer
Department of Stomatology, First Affiliated Hospital, Xinjiang Medical University, Wulumuqi 830054, China.
Ahmatjan
A
A
Suzuk
L
L
D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000401:mortality / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism / Q000401:mortality / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53