title: Thirteen single-nucleotide polymorphisms (SNPs) in the alcohol dehydrogenase 4 (ADH4) gene locus.
abstract: The human alcohol dehydrogenase 4 (ADH4) gene encodes the class II ADH4 pi subunit, which contributes to the metabolization of a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Here we report the results of systematic screening for single-nucleotide polymorphisms (SNPs) in the ADH4 gene by means of direct sequencing combined with a polymerase chain reaction method. A total of 16 genetic variations including 13 SNPs were found; 4 in the 5' flanking region, 4 in the 5' untranslated region, and 8 within introns. No variation was found in coding, 3' untranslated, or 3' flanking regions. Eight of the 13 SNPs were not reported in the NCBI dbSNP database or any previous publications. Our SNP map presented here should provide tools to evaluate the role of ADH4 in complex genetic diseases and a variety of pharmacogenetic effects.
authors.affiliation: RIKEN SNP Research Center, Institute of Medical Science, The University of Tokyo, Japan. ariiida@ims.u-tokyo.ac.jp
authors.forename: Aritoshi
authors.initials: A
authors.lastname: Iida
authors.forename: Susumu
authors.initials: S
authors.lastname: Saito
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sekine
authors.forename: Kimie
authors.initials: K
authors.lastname: Kondo
authors.forename: Chihiro
authors.initials: C
authors.lastname: Mishima
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Satoko
authors.initials: S
authors.lastname: Harigae
authors.forename: Saori
authors.initials: S
authors.lastname: Osawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D002874:Chromosome Mapping; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D000426:Alcohol Dehydrogenase; C105552:alcohol dehydrogenase IV