title: A filtration, incubation and staining reactor including a new protocol for FISH.
abstract: A newly developed device for performing fluorescence in situ hybridization (FISH) is described. An adapted procedure was compared with two typical FISH protocols. Tests were performed with Pseudomonas cells and the gene probe EUB338. With the novel procedure, we obtained a better recovery of cells and less variability in results.
authors.affiliation: Forschungszentrum Jülich, Institut für Chemie und Dynamik der Geosphäre (ICG), Institut IV: Agrosphäre, Germany. l.poschen@fz-juelich.de
authors.forename: Lothar
authors.initials: L
authors.lastname: Poschen
authors.forename: Peter
authors.initials: P
authors.lastname: Klauth
authors.forename: Joost
authors.initials: J
authors.lastname: Groeneweg
authors.forename: Ralf
authors.initials: R
authors.lastname: Wilhelm
mesh_terms: D016477:Artifacts; D005374:Filtration / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes; D011549:Pseudomonas / Q000235:genetics; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods
chemical_list: D015335:Molecular Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S