In situ localization of CD83-positive dendritic cells in psoriatic lesions.
BACKGROUND
Dendritic cells (DC) are considered to be the most potent antigen-presenting cells, and CD83 is expressed at a high level on immune-competent, activated and mature DC. Although changes in the number or localization of mature and activated CD83+ DC could be expected in psoriasis, there is little information on such changes.


OBJECTIVE
Morphological identification of CD83+ DC in psoriatic skin lesions.


METHODS
Immunohistochemical staining was performed in 5 specimens of psoriasis vulgaris and 6 specimens of pustular psoriasis. Formalin-fixed, paraffin-embedded sections were used for examination in this study. The skin sections were pretreated with 0.1% trypsin for 60 min at 37 degrees C prior to immunostaining for CD83.


RESULTS
A small but significant subpopulation of CD83+ DC was found in the upper dermis. In addition, CD83+ DC were occasionally scattered in the epidermis. The most common distribution pattern of CD83+ DC was as clusters with mononuclear lymphoid cells in the upper dermis. CD83+ DC were in close contact with lymphocytes. High-intensity staining of CD83 antigens was detected not only on the surface, but also in the cytoplasm of DC.


CONCLUSIONS
These results indicate that activated and mature CD83+ DC may play a role in the immune response in psoriasis and provide in vivo support for the concept that CD83+ DC provide signals for direct intralesional T cell activation.
Koga
Tetsuya
T
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. tekoga@dermatol.med.kyushu-u.ac.jp
Duan
Hong
H
Urabe
Kazunori
K
Furue
Masutaka
M
D015703:Antigens, CD / Q000032:analysis; D018949:Antigens, CD1 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D015415:Biomarkers; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D011565:Psoriasis / Q000276:immunology* / Q000473:pathology; D000097802:CD83 Antigen; D000097382:CD68 Molecule
D015703:Antigens, CD; D018949:Antigens, CD1; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C419393:CD1a antigen; C067980:CD68 antigen, human; D000097382:CD68 Molecule