Formes multiples de la lysyl-tARN-ligase de levure et leurs sous-unites.
When Yeast lysyl-tRNA-ligase is extracted in the presence of protease inhibitor (PMSF) a homogeneous species of enzyme is obtained, the subunits of which have a mass of 70 000 daltons. Endogenous degradation produces lighter active species of the enzyme, dimers with subunits in the range 50 000-60 000 daltons. Partial trypsinolysis mimics this degradation, which is therefore thought to occur through proteolysis.
Dimitrijevic
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Institut de Biologie Physico-chimique, 13 rue Pierre et Marie Curie, Ve, Paris, France