title: Direct polymerase chain reaction detection of Campylobacter spp. in poultry hatchery samples.
abstract: A rapid, sensitive, and specific polymerase chain reaction (PCR) assay was developed for the direct detection of Campylobacter in environmental samples from hatcheries. PCR, with a set of primers specific for the Campylobacter flaA short variable region (SVR), detected the presence of Campylobacter in both fluff and eggshell samples; however, a determination of whether the organism was living or dead could not be made. Conventional cultural methods detected no Campylobacter from the same samples. An additional benefit of the direct PCR assay is it allows for the production of a product that can be sequenced to provide further epidemiologic information.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Poultry Microbiological Safety Research Unit, Athens, GA 30604-5677, USA.
authors.forename: Kelli L
authors.initials: KL
authors.lastname: Hiett
authors.forename: Nelson A
authors.initials: NA
authors.lastname: Cox
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Stern
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D001483:Base Sequence; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002645:Chickens; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004528:Egg Shell / Q000382:microbiology; D005241:Feathers / Q000382:microbiology; D005408:Flagellin / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D005408:Flagellin; C067138:flaA protein, bacteria