Changes in the production and the effect of nitric oxide with aging in articular cartilage: an experimental study in rabbits.
We studied the production and the effect of nitric oxide (NO) in articular cartilage from rabbits of various ages. 40 New Zealand white rabbits in 4 age groups (1 month, 6 months, 1 year, and 3 years of age with 10 rabbits in each group) were used. Basal and induced levels of NO were lower in cultured chondrocytes from older rabbits. Exogenous NO administration suppressed the proliferative activity of chondrocytes to a greater degree in younger rabbits. Immunohistochemistry showed that older rabbits had fewer eNOS positive chondrocytes. Our findings imply that the age-related change in NO in articular cartilage does not have a relevance to increased NO production in osteoarthrosis.
Im
Gun-Il
GI
Department of Orthopaedics, Hallym University Hospital, Chunchon, Korea. imgunil@hanmail.net
Shin
Sung-Ryong
SR
D000375:Aging / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001707:Biopsy, Needle; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology*; D046508:Culture Techniques; D007150:Immunohistochemistry; D023421:Models, Animal; D009569:Nitric Oxide / Q000032:analysis* / Q000096:biosynthesis*; D011336:Probability; D011817:Rabbits; D012680:Sensitivity and Specificity
D009569:Nitric Oxide