title: Marker construction and cloning of a cut1-like sequence with ARS activity in the fission yeast Schizosaccharomyces japonicus.
abstract: The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non-allelic groups (genes) of mutations of the auxotrophic and mycelium-negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 (Schizosaccharomyces pombe), BimB (Aspergillus nidulans) and Esp1 (Saccharomyces cerevisiae).
authors.affiliation: Department of Genetics, University of Debrecen, Debrecen, Hungary.
authors.forename: Aniko
authors.initials: A
authors.lastname: Bozsik
authors.forename: Z
authors.initials: Z
authors.lastname: Szilagyi
authors.forename: Z
authors.initials: Z
authors.lastname: Benko
authors.forename: M
authors.initials: M
authors.lastname: Sipiczki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004261:DNA Replication; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D025282:Mycelium / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C103135:Cut1 protein, S pombe; D004271:DNA, Fungal; D005656:Fungal Proteins; D005819:Genetic Markers; D029702:Schizosaccharomyces pombe Proteins