Separation in slalom chromatography: stretching and velocity dependence.
Slalom chromatography (SC) is used for the separation of large double-stranded DNA molecules. In this technique, the progression of the DNA fragments through the closed column packing follows the flow direction and is like a snake edging is way into long grass. A novel mathematical model is developed in this paper to describe this hydrodynamic phenomenon. The results obtained provided a model for the resolution between two adjacent peaks on a chromatogram. As well, a chromatographic response function was used to obtain the most efficient separation conditions for a mixture of DNA fragments with sizes higher than 15 kbp in a minimum analysis time.
Guillaume
Yves Claude
YC
Laboratoire de Chimie Analytique, Faculté de Médecine-Pharmacie, Université de Besancon, Besançon, France.
Perrin
François Xavier
FX
Guinchard
Christiane
C
Nicod
Laurence
L
Truong
Tong Thanh
TT
Xicluna
Alain
A
Millet
Joelle
J
Thomassint
Mireille
M