title: Design, construction, and analysis of a novel class of self-folding RNA.
abstract: RNA can play multiple biological roles through use of its three-dimensional (3-D) structures. Recent advances in RNA structural biology have revealed that complex RNA 3D structures are assemblages of double-stranded helices with a variety of tertiary structural motifs. By employing RNA tertiary structural motifs together with the helices, we designed a novel class of self-folding RNA. In RNA composed of three helices (P1, P2, and P3), P1 interacts with P3 via a tetraloop-receptor interaction and P2 forms consecutive base-triples. Two designed RNAs of this class were prepared and their folding properties indicate that they form defined tertiary structures as designed. These RNAs may be used as modular units for constructing artificial ribozymes or nanometer-scale materials.
authors.affiliation: Graduate School of Biostudies, Kyoto University, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ikawa
authors.forename: Kazutake
authors.initials: K
authors.lastname: Fukada
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hideaki
authors.initials: H
authors.lastname: Shiraishi
authors.forename: Tan
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000818:Animals; D001483:Base Sequence; D008274:Magnesium / Q000378:metabolism; D008745:Methylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009153:Mutagens / Q000737:chemistry; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics; D013463:Sulfuric Acid Esters / Q000737:chemistry; D013768:Tetrahymena / Q000201:enzymology
chemical_list: D009153:Mutagens; D016337:RNA, Catalytic; D013463:Sulfuric Acid Esters; D012313:RNA; D008274:Magnesium; C007482:dimethyl sulfate