title: Goat warble fly infestation by Przhevalskiana silenus (Diptera: Oestridae): immunoepidemiologic survey in the Basilicata region (southern Italy).
abstract: The demonstration of serological cross-reactivity between the Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies led us to prepare an immunological test (ELISA) for an early diagnosis of goat warble fly infestation. Using the Hypodermosis ELISA-Kit (Vétoquinol Diagnostic, France) produced for the immunodiagnosis of bovine hypodermosis, an epidemiological assay was carried out in Basilicata region where goat breeding is very common and no data are reported with regards to the distribution of goat warble fly infestation. Out of a total of 1,100 flocks and 41,200 goats, 105 randomly extracted flocks proved to be infected and 262 sera out of 1,316 were positive; goat warble fly infestation proved to be present in Basilicata with values similar to those recorded in the surrounding regions (Apulia and Calabria). Statistical evaluation showed highly significant differences between the number of infected flocks in the mountainous areas and hills and those of the mountainous areas and the plain, but no differences between hills and plains. The higher number of positive sera and antibody titres in November-December confirmed that these months are the optimal period for sampling sera in order to perform an early diagnosis. The ELISA test was confirmed to be an easy and economic tool especially when sera sampled within a brucellosis eradication program are used.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Sezione Diagnostica Provinciale, Matera, Italy.
authors.forename: S M
authors.initials: SM
authors.lastname: Faliero
authors.forename: D
authors.initials: D
authors.lastname: Otranto
authors.forename: D
authors.initials: D
authors.lastname: Traversa
authors.forename: A
authors.initials: A
authors.lastname: Giangaspero
authors.forename: G
authors.initials: G
authors.lastname: Santagada
authors.forename: R
authors.initials: R
authors.lastname: Lia
authors.forename: V
authors.initials: V
authors.lastname: Puccini
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D003429:Cross Reactions; D004175:Diptera / Q000254:growth & development / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005843:Geography; D015511:Goat Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats / Q000469:parasitology*; D007558:Italy / Q000453:epidemiology; D007814:Larva; D008297:Male; D009198:Myiasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D015995:Prevalence; D012621:Seasons; D016036:Seroepidemiologic Studies; D013045:Species Specificity
chemical_list: D000906:Antibodies