Mutational analysis of the bglH catabolite-responsive element (cre) in Lactobacillus plantarum.
Catabolite repression of the Lactobacillus plantarum bglH gene is mediated by the catabolite control protein A (CcpA). Here we show that the binding site for the protein is a catabolite-responsive element (cre) sequence overlapping the start site of transcription. Two single and one double base substitutions in the cre sequence caused derepression of a plasmid-borne bglH-cat transcriptional fusion in L. plantarum cells grown on glucose. Analysis of the single mutations indicates that the G and C nucleotide residues in positions 2 and 13, respectively, of the 14-bp cre sequence are required for catabolite repression.
Marasco
Rosangela
R
Facoltà di Scienze MM FF NN, Università degli Studi del Sannio, via Caio Ponzio Telesino 11, 82100, Benevento, Italy.
Muscariello
Lidia
L
Rigano
Manuela
M
Sacco
Margherita
M
D001426:Bacterial Proteins; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005959:Glucosidases / Q000235:genetics* / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D005959:Glucosidases; C066586:6-phospho-beta-glucosidase