title: Improvement in the morphology of Ti-based surfaces: a new process to increase in vitro human osteoblast response.
abstract: Surface roughness has been shown to be an influencing parameter for cell response. In this experience we attempted to compare the effect of roughness organization of Ti6A14V or pure titanium substrates on human osteoblast (hOB) response (proliferation, adhesion). Surface roughness was extensively analyzed at scales above the cell size (macro-roughness) or below the cell size (micro-roughness) by calculation of relevant classic amplitude parameters (Ra, Rt) and original frequency parameters (Order, Delta). We developed a new process to prepare isotropic surfaces (electro-erosion), which were compared to isotropic surfaces obtained by polishing and anisotropic surfaces obtained by machine-tooling. The hOB response on electro-eroded (EE) Ti6A14V surfaces or pure titanium (Ti) surfaces was largely increased when compared to polished or machine-tooled surfaces after 21 days of culture. Moreover, the polygonal morphology of hOB on these EE surfaces was very close to the aspects of hOB in vivo on human bone trabeculae. By a complete description of the surface topography of EE surfaces, we concluded that when the topography was considered below the cell scale, hOB appreciated their isotropic smooth aspect, although when the topography was considered above the cell scale they appreciated their rough isotropic 'landscape' formed by many 'bowl-like nests' favouring cell adhesion and growth. Electro-erosion is a promising method for preparation of bone implant surfaces, as it could easily be applied to preparation of most biomaterials with complex geometries.
authors.affiliation: Equipe Surfaces et Interfaces ENSAM Lille, France.
authors.forename: M
authors.initials: M
authors.lastname: Bigerelle
authors.forename: K
authors.initials: K
authors.lastname: Anselme
authors.forename: B
authors.initials: B
authors.lastname: Noël
authors.forename: I
authors.initials: I
authors.lastname: Ruderman
authors.forename: P
authors.initials: P
authors.lastname: Hardouin
authors.forename: A
authors.initials: A
authors.lastname: Iost
mesh_terms: D000465:Algorithms; D016880:Anisotropy; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D016014:Linear Models; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism*; D013314:Stress, Mechanical; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D014025:Titanium