Hepatitis C virus RNA detection by in situ RT-PCR in formalin-fixed paraffin-embedded liver tissue. Comparison with serum and tissue results.
The purpose of this study was to localize HCV RNA in formalin-fixed paraffin-embedded liver biopsies of 15 patients with chronic hepatitis C using in situ RT-PCR method. The results were compared to serum and tissue extract analysis of HCV RNA. HCV RNA was detected in 80% of the sera tested, in 40% of the corresponding hepatic tissue extract and in 60% of the tissue sections tested by in situ RT-PCR. Compared to the serum positive cases, 67% of the cases were positive with in situ RT-PCR and 41% were positive with tissue extract detection. 50% of the cases in situ RT-PCR positive were also positive with tissue extract detection. These results underlined the complementarity of the different methods of viral detection for the precise diagnosis of hepatitis C.
Biagini
P
P
Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, Marseille, France. pbiagini-ets-ap@mail.guilliver.fr
Benkoel
L
L
Dodero
F
F
de Lamballerie
X
X
Chamlian
V
V
Nouhou
H
H
Gerolami
V
V
de Micco
P
P
Chamlian
A
A
D005557:Formaldehyde; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008099:Liver / Q000821:virology; D010232:Paraffin; D012367:RNA, Viral / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016610:Tissue Embedding; D016707:Tissue Fixation; D014773:Virology / Q000379:methods
D012367:RNA, Viral; D005557:Formaldehyde; D010232:Paraffin