Comparative evaluation of various commercial assays for diagnosis of dengue fever.
Dengue fever (DF) is endemic in India and dengue hemorrhagic fever (DHF) has been reported with increasing frequency in the last decade. We evaluated three commercial assays for detection of antibodies to dengue virus, to assess their performance in a diagnostic laboratory. Sera from 58 patients collected during a febrile outbreak in New Delhi in 1997 were studied. The methods evaluated were MRL Diagnostic Dengue Fever Virus IgM Capture ELISA, Pan Bio Dengue Duo IgM and IgG Capture ELISA and Pan Bio Rapid Immunochromatographic test. The MRL ELISA correctly identified 97.8% (43 of 44) of samples as dengue positive while the Pan Bio Duo ELISA and Pan Bio RIT identified 95.45% (42 of 44). The sensitivities of both Pan Bio Duo ELISA and Pan Bio RIT for primary dengue and secondary dengue were 100% and 93.54% respectively. The specificity of three assays were MRL IgM ELISA 100%, Pan Bio Duo ELISA 92.8% and Pan Bio RIT 85.7%.
Vajpayee
M
M
Department of Microbiology, All India Institute of Medical Sciences, New Delhi. mvajpayee@hotmail.com
Singh
U B
UB
Seth
P
P
Broor
S
S
D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology; D003716:Dengue Virus / Q000276:immunology; D004196:Disease Outbreaks; D006801:Humans; D007194:India / Q000453:epidemiology; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic