Deglycobleomycin: solid-phase synthesis and DNA cleavage by the resin-bound ligand.
[structure: see text] A greatly improved solid-phase synthesis of deglycobleomycin using a Dde-based linker is reported. The resin-bound deglycobleomycin could be completely deblocked and assayed for DNA plasmid relaxation, sequence-selective DNA cleavage, and light production from a molecular beacon.
Smith
Kenneth L
KL
Departments of Chemistry and Biology, University of Virginia, Charlottesville, Virginia 22901, USA.
Tao
Zhi-Fu
ZF
Hashimoto
Shigeki
S
Leitheiser
Christopher J
CJ
Wu
Xihan
X
Hecht
Sidney M
SM
D000903:Antibiotics, Antineoplastic / Q000138:chemical synthesis* / Q000737:chemistry; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008024:Ligands; D008856:Microscopy, Fluorescence; D012116:Resins, Plant
D000903:Antibiotics, Antineoplastic; D007202:Indicators and Reagents; D008024:Ligands; D012116:Resins, Plant; D001761:Bleomycin; C033284:deglycobleomycin; D004247:DNA