Toxic effect of tannic and related compounds on human plasma proteins.
OBJECTIVE
To investigate the toxicity of tannic acid related compounds such as gallic acid and polyphenol on the activity of plasma proteins in vitro. Their electrophoretic results show extremely important information, albumin and globulin levels are remarkably changed and characterized by disorder in their fractions avere which occurred frequently.


METHODS
All plasma proteins samples of sets A, B and C were treated in sequences with known different concentrations of gallic acid, gallotannin and polypholes, which were separated chromatographically from phenolic extract of fruit peel of punicaceae. These were then treated, A, B and C were subjected to electrophoresis techniques, for identification and quantitation.


RESULTS
The electrophoretic patterns of treated plasma proteins samples, sets A, B and C are arised with remarkable changes in their fraction levels, compared to normal. The results in were also characterized by disorders in their electrophoretic pattern. In this way 5 fractions of treated plasma proteins could be distinguished after sustaining which are ablumin and alpha1, alpha2 and beta and gamma globulins.


CONCLUSIONS
The biological activity of tannic acid related compounds on plasma proteins in vitro, is important in determining their toxicity, and this toxicity may be depend upon their metabolic processes in the liver. In addition, the electrophoretic techniques used for separation and identification of plasma protein is extremely important for future work in the area.
Al-Shafi
Sabah M
SM
Department of Clinical and Laboratory Sciences, College of Pharmacy, University of Baghdad, Baghdad, Iraq. alshafi_sabah@hotmail.com
D001798:Blood Proteins / Q000187:drug effects*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D047348:Hydrolyzable Tannins / Q000633:toxicity*; D066298:In Vitro Techniques; D007493:Iraq; D010636:Phenols / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*
D001798:Blood Proteins; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts