A novel non-conventional heat shock element regulates expression of MDJ1 encoding a DnaJ homolog in Saccharomyces cerevisiae.
The heat shock factor (HSF) is a pivotal transcriptional factor that regulates the expression of genes encoding heat shock proteins (HSPs) via heat shock elements (HSEs). nGAAnnTTCnnGAAn functions as the minimum consensus HSE (cHSE) in vivo. Here we show that the expression of Saccharomyces cerevisiae MDJ1 encoding a mitochondrial DnaJ homolog is regulated by HSF via a novel non-consensus HSE (ncHSE(MDJ1)), which consists of three separated pentameric nGAAn motifs, nTTCn-(11 bp)-nGAAn-(5 bp)-nGAAn. This is the first evidence to show that the immediate contact of nGAAn motifs is dispensable for regulation by HSF in vivo. ncHSE(MDJ1) confers different heat shock responses versus cHSE and, unlike cHSE, definitively requires a carboxyl-terminal activation domain of HSF in the expression. ncHSE(MDJ1)-like elements are found in promoter regions of some other DnaJ-related genes. The highly conserved HSF/HSE system suggests that similar ncHSEs may be used for the expression of HSP genes in other eukaryotes including humans.
Tachibana
Tomohusa
T
Department of Biology and Geoscience, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan.
Astumi
Shiho
S
Shioda
Ryo
R
Ueno
Masaru
M
Uritani
Masahiro
M
Ushimaru
Takashi
T
D020816:Amino Acid Motifs; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017353:Gene Deletion; D017930:Genes, Reporter; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D004268:DNA-Binding Proteins; C067168:HSF1 protein, S cerevisiae; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C087041:MDJ1 protein, S cerevisiae; D008565:Membrane Proteins; D009841:Oligonucleotides; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase