[Molecular genetic methods for the detection of rifampicin-resistant Mycobacterium tuberculosis strains].
RCR-heteroduplex (GDA) and chip methods were used to detect rifampricin-resistant (RR) and rifampicin-sensitive (RS) Mycobacterium tuberculosis (MTB) in the samples from patients (sputum) and in the clinical isolates of MTB from these patients (MB/BacT liquid medium and Lowenstein Jensen's (LJ) solid medium. The efficiency of detecting RR and RS of MTB (from the sputum) is 100 and 92.3% in the chip and GDA tests, respectively. Correlations between GDA (sputum) and drug test (LJ) were 91.7%, that of chip (sputum) and drug test LJ, 88.5%, chip (sputum) and chip clinical isolates (LJ), 100%. The efficacy of GDA and chip in the detection of RR of MTB strains is under discussion.
Skotnikova
O I
OI
Soboleva
A Iu
AIu
Mikhaĭlovich
V M
VM
Griadunov
D A
DA
Irtuganova
O A
OA
Nosova
E Iu
EIu
Isaeva
E L
EL
Lapa
S A
SA
Zasedatelev
A S
AS
Litvinov
V I
VI
Moroz
A M
AM
Mirzabekov
A D
AD
D000995:Antitubercular Agents / Q000627:therapeutic use*; D004352:Drug Resistance, Microbial; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D009164:Mycobacterium Infections / Q000235:genetics* / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics; D012293:Rifampin / Q000627:therapeutic use*; D014376:Tuberculosis / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology*
D000995:Antitubercular Agents; D012293:Rifampin
Molekuliarno-geneticheskie metody vyiavleniia rifampitsinrezistentnykh shtammov Mycobacterium tuberculosis.