Structure of POIA1, a homologous protein to the propeptide of subtilisin: implication for protein foldability and the function as an intramolecular chaperone.
Solution structure of POIA1 (Pleurotus ostreatus proteinase A inhibitor 1), which functions as an intramolecular chaperone and as an inhibitor to subtilisin, was determined. By making use of the fact that POIA1 is the only structured protein that shows homology to the propeptide of subtilisin, which is unstructured by itself, foldability of this protein was elucidated. It became clear that the evolutionarily conserved residues play two important roles, one for the maintenance of its own structure, and the other for the interaction with subtilisin. Structural softness and mutational tolerance contained in the POIA1 structure makes it an ideal material for designing a foldable protein.
Sasakawa
Hiroaki
H
PRESTO, JST (Japan Science and Technology Cooperation), Nada, Kobe 657-8501, Japan.
Yoshinaga
Sosuke
S
Kojima
Shuichi
S
Tamura
Atsuo
A
D000595:Amino Acid Sequence; D017124:Conserved Sequence; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors / Q000737:chemistry*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000737:chemistry*; D020076:Pleurotus / Q000737:chemistry* / Q000235:genetics; D011480:Protease Inhibitors / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D012996:Solutions; D013381:Subtilisins / Q000037:antagonists & inhibitors / Q000737:chemistry*
D004792:Enzyme Precursors; D005656:Fungal Proteins; D018832:Molecular Chaperones; C018134:PAI3 protein, S cerevisiae; D010446:Peptide Fragments; D011480:Protease Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D012996:Solutions; C057107:prosubtilisin; D013381:Subtilisins