A dorsal root ganglia cell line derived from trisomy 16 fetal mice, a model for Down syndrome.
We have established two immortalized cell lines from dorsal root ganglia of normal (G4b) and trisomy 16 mice (GT1), a model for Down syndrome. By immunohistochemistry, both cell lines exhibit neuronal traits and lack glial markers. GTl cells exhibited greater [3H]choline uptake than G4b cells. K+ and nicotine-mediated acetylcholine release was greater in GT1 cells. Basal intracellular Ca2+ concentration ([Ca2+]i) was significantly lower in GTl cells. More GTl cells responded to neurotransmitters with a transient [Ca2+]i increase compared to G4b cells, but both cell types showed similar amplitudes of [Ca2+]i responses. The results show that both cell lines retain neuronal characteristics and respond to specific neurotransmitter stimuli. Altered GT1 cell responses could be related to neuronal pathophysiology in Down's syndrome.
Allen
David D
DD
Department of Pharmaceutical Sciences, Texas Tech University HSC, Amarillo, Texas, USA.
Cárdenas
Ana María
AM
Arriagada
Christian
C
Bennett
Lori B
LB
García
Carlos J
CJ
Caviedes
Raúl
R
Rapoport
Stanley I
SI
Caviedes
Pablo
P
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002461:Cell Line, Transformed; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D004195:Disease Models, Animal; D004314:Down Syndrome / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005333:Fetus; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011247:Pregnancy; D014314:Trisomy / Q000235:genetics*
D002118:Calcium