Green tea catechins decrease apolipoprotein B-100 secretion from HepG2 cells.
To understand the hypocholesterolemic activity of green tea, our in vitro studies screened the relative efficacy of two structurally distinct green tea catechins, epicatechin (EC) and epigallocatechin gallate (EGCG), on apolipoprotein B-100 (apoB) and lipid production using a well established human hepatoma cell-line, HepG2, as the model system. This study showed that HepG2 cells pretreated with EC and EGCG for 8 h exerted a dose-dependent inhibitory effect on apoB secretion. Total protein and albumin synthesis and secretion were unaffected indicating the effects on apoB secretion to be specific. Under lipid-rich conditions, apoB secretion was markedly reduced by EGCG and to a lesser extent by EC at 50 microM. Mechanistic study showed that tea catechins inhibited apoB secretion via a proteasome-independent pathway as indicated by a lack of response to N-acetyl-leucyl-leucyl-norleucinal (ALLN), a proteasome inhibitor. The effect on apoB secretion was also found to be independent of lipid biosynthesis. In summary, the data suggest that EGCG in contrast to EC is a potent inhibitor of apoB secretion. The results indicate that the gallate moiety in the catechin molecule may result in a beneficial effect on lipid metabolism in terms of apoB secretion.
Yee
Winnie L
WL
Division of Medical Technology, University of Hawaii, Honolulu 96822, USA.
Wang
Qi
Q
Agdinaoay
Trecito
T
Dang
Kaohimanu
K
Chang
Healani
H
Grandinetti
Andrew
A
Franke
Adrian A
AA
Theriault
Andre
A
D000924:Anticholesteremic Agents / Q000494:pharmacology; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015243:Cholesterol, VLDL / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism; D006801:Humans; D006888:Hydroxycholesterols / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D009097:Multienzyme Complexes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D013662:Tea; D014407:Tumor Cells, Cultured
D000924:Anticholesteremic Agents; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D015243:Cholesterol, VLDL; D006888:Hydroxycholesterols; D008055:Lipids; D009097:Multienzyme Complexes; D013662:Tea; C007997:25-hydroxycholesterol; D002392:Catechin; C045651:epigallocatechin gallate; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex