Application of immunoelectron microscopy techniques in the diagnosis of phytoplasma diseases.
An immunoelectron microscopy technique was applied to label Chrysanthemum leuchanthemum phytoplasma in infected leaf tissues of Chrysanthemum leuchanthemum L. and Catharanthus roseus L. plants. Specific monoclonal antibodies at different dilutions and secondary antimouse antibody conjugated with colloidal gold particles of different sizes were used. The monoclonal antibodies demonstrated their specificity against the antigen; immunocytological methods permitted the precise localization and identification of phytoplasmas in thin sections from infected tissues.
Musetti
Rita
R
Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, 33100 Udine, Italy. Rita.Musetti@pldef.uniud.it
Loi
Nazia
N
Carraro
Luigi
L
Ermacora
Paolo
P
D000911:Antibodies, Monoclonal; D011723:Chrysanthemum cinerariifolium / Q000382:microbiology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D008973:Tenericutes / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal