title: Lack of isoprene overproduction during peritoneal dialysis.
abstract: OBJECTIVE
  Isoprene is the constitutive unit of isoprenoid lipids and sterols. However, it is also a potential toxic and carcinogenic agent. Recent findings of a marked and prolonged isoprene overproduction induced by hemodialysis sessions raises the question of isoprene behavior in patients on peritoneal dialysis.
  
  
  METHODS
  A study with repeated measures per patient and healthy control.
  
  
  METHODS
  Nephrology and Dialysis Unit and Perugia University Medical School.
  
  
  METHODS
  Sixteen consecutive patients on regular continuous ambulatory peritoneal dialysis (CAPD) were evaluated. Endogenous isoprene was analyzed using gas chromatographic assay of breath isoprene, collected at set times before and after dialysis fluid exchange.
  
  
  RESULTS
  No significant variations were found in breath isoprene concentrations in the different samples from each patient, and levels were almost stable within the normal range of healthy controls.
  
  
  CONCLUSIONS
  These results show that CAPD, unlike hemodialysis, has little or no effect on isoprene and isoprenoid-related lipid turnover. This lack of increased endogenous isoprene synthesis, in addition to being a distinctive metabolic feature of CAPD, could have important pathophysiological and clinical implications.
authors.affiliation: Department of Clinical and Experimental Medicine, Perugia University Medical School, Italy. encap@unipg.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Capodicasa
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Trovarelli
authors.forename: Federica
authors.initials: F
authors.lastname: Brunori
authors.forename: Luigi
authors.initials: L
authors.lastname: Vecchi
authors.forename: Carmen
authors.initials: C
authors.lastname: Carobi
authors.forename: MedioGiannaE
authors.initials: M
authors.lastname: De
authors.forename: Maria A
authors.initials: MA
authors.lastname: Pelli
authors.forename: Umberto
authors.initials: U
authors.lastname: Buoncristiani
mesh_terms: D000328:Adult; D000368:Aged; D001944:Breath Tests; D002070:Butadienes / Q000032:analysis / Q000378:metabolism*; D002940:Circadian Rhythm; D005260:Female; D045782:Hemiterpenes; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010420:Pentanes; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013997:Time Factors
chemical_list: D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene