Simultaneous differentiation and typing of Entamoeba histolytica and Entamoeba dispar.
Sequences corresponding to some of the polymorphic loci previously reported from Entamoeba histolytica have been detected in Entamoeba dispar. Comparison of nucleotide sequences of two loci between E. dispar strain SAW760 and E. histolytica strain HM-1:IMSS revealed significant differences in both repeat and flanking regions. The tandem repeat units varied not only in sequence but also in number and arrangement between the two species at both the loci. Using the sequences obtained, primer pairs aimed at amplifying species-specific products were designed and tested on a variety of E. histolytica and E. dispar samples. Amplification results were in complete agreement with the original species classification in all cases, and the PCR products displayed discernible size and pattern variations among the isolates.
Zaki
Mehreen
M
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.
Meelu
Parool
P
Sun
Wei
W
Clark
C Graham
CG
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis; D004747:Entamoeba / Q000145:classification* / Q000235:genetics; D004748:Entamoeba histolytica / Q000145:classification* / Q000235:genetics; D004749:Entamoebiasis / Q000469:parasitology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020080:Tandem Repeat Sequences / Q000235:genetics
D017931:DNA Primers; D016054:DNA, Protozoan