Miniature surface-plasmon resonance immunosensors--rapid and repetitive procedure.
Miniaturized immunosensors based on surface-plasmon resonance (SPR) have been developed by using the sensor recently developed by Texas Instruments. By using this sensor for human immunoglobulin G (IgG) rapid and repetitive measurements could be performed by alternate injection of sample and glycine-HCl buffer. A sensor for human serum albumin (HSA) could also be developed similarly. One difficulty in immunosensor development is dissociation of the strongly bound antigen-antibody complex. A "regenerable" immunosensor has also been developed for this reason.
Suzuki
Masayasu
M
Department of Electric and Electronic Engineering, Toyama University, Japan.
Ozawa
Fumihiko
F
Sugimoto
Wakako
W
Aso
Shuji
S
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002138:Calibration; D004867:Equipment Design; D017743:Equipment Reuse; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008904:Miniaturization; D012709:Serum Albumin / Q000032:analysis; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D013997:Time Factors
D007074:Immunoglobulin G; D007136:Immunoglobulins; D012709:Serum Albumin