Rainbow trout neutrophils are responsible for non-specific cytotoxicity.
This is the first report that rainbow trout (Oncorhynchus mykiss) neutrophils are responsible for non-specific cytotoxicity. A monoclonal antibody (mab) for rainbow trout leucocytes was produced. Using this mab (TTL-5E9), neutrophils (5E9-positive cells) were isolated from the pronephros by a panning technique. The isolated neutrophils showed high viability (approximately 95%) and purity (92-95%), and were functional in cytotoxic activity assays. The neutrophils demonstrated significantly higher cytotoxic activities against YAC-1 target cells than the other cells (5E9-negative cells, predominantly lymphocytes). The number of neutrophils contaminating the 5E9-negative fraction and their non-specific cytotoxicities were positively correlated. These findings demonstrate that rainbow trout neutrophils possess non-specific cytotoxic activities.
Sasaki
Yurie
Y
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
Maita
Masashi
M
Okamoto
Nobuaki
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002469:Cell Separation / Q000662:veterinary; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005434:Flow Cytometry / Q000662:veterinary; D009504:Neutrophils / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal