title: Quantitative structure-retention relationships in affinity high-performance liquid chromatography.
abstract: In this report the affinity high-performance liquid chromatography data, which were determined on silica-based human serum albumin, alpha1-acid glycoprotein, keratin, collagen, melanin, amylose tris(3,5-dimethylphenylcarbamate), and basic fatty acid binding protein columns, are discussed. Using a quantitative structure-retention relationship (QSRR) approach the affinity data were interpreted in terms of structural requirements of specific binding sites on biomacromolecules. The unique chromatographic properties of immobilized artificial membrane and cholesterol stationary phases were also analyzed from the point of view of mimicking biological processes. It has been demonstrated that chemometric processing of appropriately designed sets of chromatographic data derived in systems comprising biomolecules provides information of relevance for molecular pharmacology and rational drug design.
authors.affiliation: Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Markuszewski
authors.forename: Roman
authors.initials: R
authors.lastname: Kaliszan
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D011506:Proteins / Q000737:chemistry; D021281:Quantitative Structure-Activity Relationship
chemical_list: D008567:Membranes, Artificial; D011506:Proteins; D002784:Cholesterol