Effects of natural phenolic compounds on the antioxidant activity of lactoferrin in liposomes and oil-in-water emulsions.
The effect of natural phenolic compounds on the antioxidant and prooxidant activity of lactoferrin was studied in liposomes and oil-in-water emulsions containing iron. The antioxidants tested with lactoferrin were alpha-tocopherol, ferulic acid, coumaric acid, tyrosol, and natural phenolic extracts obtained from three different extra-virgin olive oils and olive mill wastewater. The natural extracts of olive oils and mill wastewaters were composed mainly of polyphenols and simple phenolics, respectively. Lipid oxidation at 30 degrees C was determined by the formation of hydroperoxides and fluorescent compounds resulting from oxidized lipid interactions. All phenolic compounds showed synergistic properties in reinforcing the antioxidant activity of lactoferrin in lipid systems containing iron. The highest synergistic effects were observed for the phenolic extracts rich in polyphenols of extra-virgin olive oils and lactoferrin. This synergistic effect was higher in liposomes than in emulsions.
Medina
Isabel
I
Instituto de Investigaciones Marinas del CSIC, Eduardo Cabello 6, E-36208 Vigo, Spain. medina@iim.csic.es
Tombo
Isabel
I
Satué-Gracia
M Teresa
MT
German
J Bruce
JB
Frankel
Edwin N
EN
D000975:Antioxidants / Q000494:pharmacology*; D001639:Bicarbonates / Q000494:pharmacology; D004655:Emulsions / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007781:Lactoferrin / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes / Q000378:metabolism*; D000069463:Olive Oil; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry; D014867:Water / Q000737:chemistry; D024502:alpha-Tocopherol
D000975:Antioxidants; D001639:Bicarbonates; D004655:Emulsions; D008081:Liposomes; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D014867:Water; D007501:Iron; D007781:Lactoferrin; D024502:alpha-Tocopherol