title: The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.
abstract: The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unknown mechanism that does not involve cohesin cleavage. We show that Polo-like kinase is required for the cleavage-independent dissociation of cohesin from chromosomes in Xenopus. Cohesin phosphorylation depends on Polo-like kinase and reduces the ability of cohesin to bind to chromatin. These results suggest that Polo-like kinase regulates the dissociation of cohesin from chromosomes early in mitosis.
authors.affiliation: Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sumara
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Vorlaufer
authors.forename: P Todd
authors.initials: PT
authors.lastname: Stukenberg
authors.forename: Olaf
authors.initials: O
authors.lastname: Kelm
authors.forename: Norbert
authors.initials: N
authors.lastname: Redemann
authors.forename: Erich A
authors.initials: EA
authors.lastname: Nigg
authors.forename: Jan-Michael
authors.initials: JM
authors.lastname: Peters
mesh_terms: D000818:Animals; D064127:Aurora Kinases; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002458:Cell Fractionation; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D014020:Tissue Extracts / Q000737:chemistry / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
chemical_list: D064127:Aurora Kinases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D014020:Tissue Extracts; D029867:Xenopus Proteins; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C112134:AURKA protein, Xenopus