title: Bone marrow-derived mast cell differentiation is strongly reduced in histidine decarboxylase knockout, histamine-free mice.
abstract: Mast cells are differentiated in vitro from bone marrow precursors. In this study the development of bone marrow-derived mast cells was examined from histidine decarboxylase deficient (HDC-/-) and wild-type mice in the presence of IL-3. The number of non-adherent, tryptase- and c-kit-positive mast cells in bone marrow-derived cultures of HDC(-/-) mice was decreased compared to that of wild-type (HDC+/+) animals, but within the tryptase- and c-kit-positive cells there was no difference in the expression intensity of both markers between the two groups. Furthermore, less serine proteases mMCP5, mMCP6 and FcepsilonRIalpha mRNA were detected in bone marrow-derived cell cultures originating from HDC-/- mice. Antigen-provoked degranulation through high-affinity FcepsilonI receptor was also lower in HDC-/- mice. The colony assays in semisolid medium yielded a significantly lower ratio of mixed colonies and higher proportion of macrophage colonies from HDC-/- mice-derived bone marrow compared to the wild-type. In the course of the differentiation of HDC-/- --derived mast cells exogenously added histamine is unable to substitute the endogenously missing histamine. Concordantly, alpha-fluoromethyl-histamine, the specific inhibitor of HDC, revealed only a marginal inhibition on the differentiation of tryptase-positive mast cells from wild-type mice. These findings suggest that the effect of histamine on the IL-3-dependent development of bone marrow-derived mast cell differentiation during the early period is crucial and irreplaceable.
authors.affiliation: Department of Genetics, Cell and Immunobiology, Faculty of Medicine, Semmelweis University, 1089 Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Wiener
authors.forename: Márton
authors.initials: M
authors.lastname: Andrásfalvy
authors.forename: Eva
authors.initials: E
authors.lastname: Pállinger
authors.forename: Péter
authors.initials: P
authors.lastname: Kovács
authors.forename: Csaba
authors.initials: C
authors.lastname: Szalai
authors.forename: Anna
authors.initials: A
authors.lastname: Erdei
authors.forename: Sára
authors.initials: S
authors.lastname: Tóth
authors.forename: András
authors.initials: A
authors.lastname: Nagy
authors.forename: András
authors.initials: A
authors.lastname: Falus
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001853:Bone Marrow / Q000502:physiology*; D002448:Cell Adhesion; D015550:Cell Degranulation; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D006640:Histidine Decarboxylase / Q000235:genetics / Q000502:physiology*; D007377:Interleukin-3 / Q000502:physiology; D008297:Male; D008407:Mast Cells / Q000201:enzymology / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D012697:Serine Endopeptidases / Q000378:metabolism; D053802:Tryptases
chemical_list: D000954:Antigens, Surface; D007377:Interleukin-3; C509190:Tpsb2 protein, mouse; D006632:Histamine; D012697:Serine Endopeptidases; C509222:Tpsab1 protein, mouse; D053802:Tryptases; D006640:Histidine Decarboxylase