title: Conformational change of the E-F interhelical loop in the M photointermediate of bacteriorhodopsin.
abstract: The conformation of the structured EF interhelical loop of bacteriorhodopsin and its change in the M photointermediate were assessed by measuring the rate of reaction of 16 single engineered cysteine residues along the loop with water-soluble sulfhydryl reagents. The exposure to the bulk in the unilluminated state determined with the cysteine reaction correlated well with the degree of access to water calculated from the crystallographic structure of the loop. The EF-loop should be affected by the well-known outward tilt of helix F in the M and N intermediates of the photocycle. A second mutation in each cysteine mutant, the D96N residue replacement, allowed full conversion to the M state by illumination. The reaction rates measured under these conditions indicated that buried residues tend to become more exposed, and exposed residues become more buried in M. This is to be expected from tilt of helix F. However, the observation of increased exposure of four residues near the middle of the loop, where steric effects are only from other loop residues, indicate that the conformation of the EF-loop itself is changed. Thus, the motion of the loop in M is more complex than expected from simple tilt of helix F, and may include rotation that unwinds its twist.
authors.affiliation: Department of Physiology & Biophysics, University of California, Irvine, CA 92697, USA.
authors.forename: Leonid S
authors.initials: LS
authors.lastname: Brown
authors.forename: Richard
authors.initials: R
authors.lastname: Needleman
authors.forename: Janos K
authors.initials: JK
authors.lastname: Lanyi
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D003624:Darkness; D004220:Disulfides; D004228:Dithionitrobenzoic Acid; D019617:Halobacterium salinarum / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D011487:Protein Conformation; D015202:Protein Engineering; D011725:Pyridines; D012399:Rotation
chemical_list: D004220:Disulfides; D011725:Pyridines; C007542:4,4'-dipyridyl disulfide; D001436:Bacteriorhodopsins; D004228:Dithionitrobenzoic Acid; D003545:Cysteine