Fluorescence anisotropy near-field scanning optical microscopy (FANSOM): a new technique for nanoscale microviscometry.
A near-field scanning optical microscope system was implemented and adapted for nanoscale steady-state fluorescence anisotropy measurement. The system as implemented can resolve approximately 0.1 cP microviscosity variations with a resolution of 250 nm laterally in the near field, or approximately 10 microm when employed in a vertical scanning mode. The system was initially used to investigate the extent of microviscous vicinal water over surfaces of varying hydrophilicity. Water above a cleaved mica surface was found to have a decreased microviscosity, while water above a hydrophobic surface showed no change (detection limit approximately 0.1 cP at approximately 30 + nm from the surface).
Reitz
Frederick B
FB
Department of Bioengineering, University of Washington, Seattle 98195-7962, USA.
Fauver
Mark E
ME
Pollack
Gerald H
GH
D019793:Fluorescein; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D012996:Solutions; D014783:Viscosity; D014867:Water
D005456:Fluorescent Dyes; D012996:Solutions; D014867:Water; D019793:Fluorescein