title: [Molecular genetic methods for the detection of rifampicin-resistant Mycobacterium tuberculosis strains].
abstract: RCR-heteroduplex (GDA) and chip methods were used to detect rifampricin-resistant (RR) and rifampicin-sensitive (RS) Mycobacterium tuberculosis (MTB) in the samples from patients (sputum) and in the clinical isolates of MTB from these patients (MB/BacT liquid medium and Lowenstein Jensen's (LJ) solid medium. The efficiency of detecting RR and RS of MTB (from the sputum) is 100 and 92.3% in the chip and GDA tests, respectively. Correlations between GDA (sputum) and drug test (LJ) were 91.7%, that of chip (sputum) and drug test LJ, 88.5%, chip (sputum) and chip clinical isolates (LJ), 100%. The efficacy of GDA and chip in the detection of RR of MTB strains is under discussion.
authors.forename: O I
authors.initials: OI
authors.lastname: Skotnikova
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Soboleva
authors.forename: V M
authors.initials: VM
authors.lastname: Mikhaĭlovich
authors.forename: D A
authors.initials: DA
authors.lastname: Griadunov
authors.forename: O A
authors.initials: OA
authors.lastname: Irtuganova
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Nosova
authors.forename: E L
authors.initials: EL
authors.lastname: Isaeva
authors.forename: S A
authors.initials: SA
authors.lastname: Lapa
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: V I
authors.initials: VI
authors.lastname: Litvinov
authors.forename: A M
authors.initials: AM
authors.lastname: Moroz
authors.forename: A D
authors.initials: AD
authors.lastname: Mirzabekov
mesh_terms: D000995:Antitubercular Agents / Q000627:therapeutic use*; D004352:Drug Resistance, Microbial; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D009164:Mycobacterium Infections / Q000235:genetics* / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics; D012293:Rifampin / Q000627:therapeutic use*; D014376:Tuberculosis / Q000175:diagnosis / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D012293:Rifampin
vernacular_title: Molekuliarno-geneticheskie metody vyiavleniia rifampitsinrezistentnykh shtammov Mycobacterium tuberculosis.