Bio-barcodes based on oligonucleotide-modified nanoparticles.
By utilizing oligonucleotide-modified Au nanoparticles encoded with sequences that act as biobarcodes, one can screen for multiple target polyvalent proteins simultaneously in one solution. This novel concept was demonstrated with two types of detection formats, a homogeneous assay and one based on oligonucleotide microarrays. With such an approach, one can prepare an extraordinarily large number of barcodes from synthetically accessible oligonucleotides (e.g., a 12-mer sequence offers 4(12) possible barcodes).
Nam
Jwa-Min
JM
Northwestern University, Department of Chemistry and Institute for Nanotechnology, Evanston, Illinois 60208, USA.
Park
So-Jung
SJ
Mirkin
Chad A
CA
D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D009841:Oligonucleotides / Q000737:chemistry*
D007074:Immunoglobulin G; D009841:Oligonucleotides; D007073:Immunoglobulin E; D004247:DNA