Simple and sensitive determination of citrinin in Monascus by GC-selected ion monitoring mass spectrometry.
A new method for the qualitative and quantitative analysis of citrinin in Monascus by gas-chromatography-selected ion monitoring (SIM) mass spectrometry has been developed. GC separation of citrinin in Monascus extract was achieved without the need for chemical derivatization, and could be detected as a single peak when the SIM mode selected 5 prominent fragmentations (m/z of 220, 205, 177, 105 and 91). The quantitative detection limit for citrinin was approximately 1 ppb. Finally, the GC-separated analyte from Monascus extract, at a retention time of 10.89 min, was examined by the method of pattern recognition by comparison with a citrinin standard. The results show that the 2 compounds had a 94% similarity when the SIM mode was used.
Shu
Pin-Yen
PY
Department of Chemistry, National Taiwan Normal University, Taipei.
Lin
Cheng-Huang
CH
D001203:Ascomycota / Q000737:chemistry*; D002138:Calibration; D002953:Citrinin / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002953:Citrinin