Feeding different amounts of colostrum or only milk replacer modify receptors of intestinal insulin-like growth factors and insulin in neonatal calves.
Colostrum intake influences growth and development of the gastrointestinal tract (GIT) in several species and colostral insulin-like growth factors I and II (IGF-I and IGF-II), and insulin are involved in neonatal intestinal tissue growth. We have studied IGF type 1, IGF type 2, and insulin receptors in the intestine of 8-day-old calves fed different amounts of colostrum or only milk replacer. Calves were fed colostrum of the first six milkings on the first 3 days and then milk replacer (GrC(6)) or colostrum only once and then milk replacer (GrC(1)) or they were fed only milk replacer from the beginning, i.e., no colostrum (GrM). Competitive binding studies and ligand blots confirmed the presence of IGF type 1, IGF type 2, and insulin receptors in mucosal cell membranes of duodenum, jejunum, ileum, and colon. The IGF type 1 receptor number in ileum and total intestine in GrC(6) was greater (P<0.05) than in GrC(1) and in GrM, and IGF type 2 receptor number in total intestine was greater (P<0.05) in GrC(6) than in GrM. Insulin binding was best fitted by a model with two binding sites. High affinity insulin receptor numbers in duodenum, ileum, and total intestine were greater (P<0.05) in GrC(6) than in GrM. The data demonstrate that IGF type 1, IGF type 2, and insulin receptors in intestinal mucosa of neonatal calves are influenced by feeding.
Hammon
H M
HM
Faculty of Veterinary Medicine, Division of Animal Nutrition and Physiology, Institute of Animal Genetics, Nutrition and Housing, University of Berne, CH-3012 Berne, Switzerland. harald.hammon@itz.unibe.ch
Blum
J W
JW
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001667:Binding, Competitive; D002417:Cattle / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry; D003126:Colostrum; D005526:Food, Formulated; D007328:Insulin / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D007413:Intestinal Mucosa / Q000737:chemistry; D007457:Iodine Radioisotopes; D008892:Milk; D011869:Radioligand Assay; D017526:Receptor, IGF Type 1 / Q000032:analysis* / Q000378:metabolism; D017527:Receptor, IGF Type 2 / Q000032:analysis* / Q000378:metabolism; D011972:Receptor, Insulin / Q000032:analysis* / Q000378:metabolism
D007328:Insulin; D007457:Iodine Radioisotopes; D017527:Receptor, IGF Type 2; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin