title: Deoxyribozyme-based logic gates.
abstract: We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open the possibility of communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.
authors.affiliation: Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York 10032, USA. mns18@columbia.edu
authors.forename: Milan N
authors.initials: MN
authors.lastname: Stojanovic
authors.forename: Tiffany Elizabeth
authors.initials: TE
authors.lastname: Mitchell
authors.forename: Darko
authors.initials: D
authors.lastname: Stefanovic
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry*; D015195:Drug Design; D008128:Logic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D021881:DNA, Catalytic; D009841:Oligonucleotides