Stress-70 as indicator of heavy metals accumulation in blue mussel Mytilus edulis.
The induction of stress-70 (Hsp70) in the blue mussels Mytilus edulis from southern Baltic after exposure to cadmium, lead and copper, administrated individually and/or as a mixture, was investigated by means of standard Western blotting using a clone 3A3 monoclonal antibody. The general course of the stress-70 induction was found to be similar for all tested metals. It could be characterised by three main features: (1) The induction of stress-70 proteins was only slightly increased at low metal concentration. (2) Strong induction of stress-70 occurred at high concentration of metals. Combination of metals (Cd+Cu) was found to increase the stress-70 level more intensely than related concentrations of singularly applied metals. (3) Five weeks of long depuration had no effect on stress-70 levels. Experiments revealed different induction of stress-70 as a response to cadmium, lead and copper exposure and confirm that the applied technique might serve as a versatile tool to assess invertebrate stress reaction to environmentally relevant heavy metals.
Radlowska
M
M
Institute of Oceanology, Sopot, Poland.
Pempkowiak
J
J
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000201:enzymology*; D015153:Blotting, Western; D004781:Environmental Exposure; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000096:biosynthesis; D019216:Metals, Heavy / Q000009:adverse effects / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D019216:Metals, Heavy