[Validation of the HPLC method in the determination of dioxopromethazine and phenylephrine in eye drops].
The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanol/1.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 ml/min, sample volume 20 microliters, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a ratio of 1:50. The elaborated HPLC method and the chromatographic system were validated according to the procedure for the validation of chromatographic systems and methods.
Hudecová
T
T
Katedra farmaceutickej analýzy a nukleárnej farmácie Farmaceutickej fakulty Univerzity Komenského, Bratislava. hudecova@fpharm.uniba.sk
Hatrík
S
S
Zimová
N
N
Havránek
E
E
D018926:Anti-Allergic Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000032:analysis*; D009883:Ophthalmic Solutions / Q000737:chemistry*; D010656:Phenylephrine / Q000032:analysis*; D011398:Promethazine / Q000031:analogs & derivatives* / Q000032:analysis*; D013566:Sympathomimetics / Q000032:analysis; D014662:Vasoconstrictor Agents / Q000032:analysis*
D018926:Anti-Allergic Agents; D006634:Histamine H1 Antagonists; D009883:Ophthalmic Solutions; D013566:Sympathomimetics; D014662:Vasoconstrictor Agents; D010656:Phenylephrine; C007508:dioxopromethazine; D011398:Promethazine
Validácia HPLC metódy pre stanovenie dioxoprometazínu a fenylefrínu v ocnej instilácii.