Detection of infectious agents in laboratory rodents: traditional and molecular techniques.
Methods to detect infectious agents in laboratory animals have traditionally been serological and culture based. Molecular methods to detect infectious agents in laboratory animals are being used more routinely. Confusion as to when and how to use molecular methods abounds. In this review, we present a guide to the weaknesses and strengths of using traditional and molecular methods for the detection of infectious agents in laboratory animals.
Compton
S R
SR
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Riley
L K
LK
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D001431:Bacteriological Techniques; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary; D016481:Helicobacter Infections / Q000175:diagnosis / Q000662:veterinary; D006524:Hepatitis, Viral, Animal / Q000175:diagnosis; D051379:Mice; D006517:Murine hepatitis virus; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D012680:Sensitivity and Specificity; D012699:Serology / Q000379:methods; D013048:Specimen Handling; D014773:Virology / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*