title: Uptake of transition metal ions using liposomes containing dicetylphosphate as a ligand.
abstract: The uptake of Cu2+ was investigated using various types of liposomes composed of phosphatidylcholine (PC), cholesterol (Chol) and dicethylphosphate (DCP). DCP played a role as a ligand for Cu2+. Multilamellar vesicles (MLVs) were more effective for the uptake of Cu2+ compared to unilamellar vesicles prepared by the extrusion technique. The uptake efficiency of MLVs for Cu2+ was dependent on the molar ratio of DCP in MLVs. The uptake percent of Cu2+ was 92% using MLVs having a PC:DCP:Chol molar ratio of 4:3:3; 95% of the total vesicle Cu2+ was bound to DCP of the outer membrane surface of the MLVs, and the remaining 5% of the total Cu2+ was distributed into the interior side of the MLVs. MLVs having a PC:DCP:Chol molar ratio of 4:3:3 were also effective as separation media for Mn2+, Co2+, Ni2+ and Zn2+. The uptake efficiency of the MLVs for the transition-metal ions increased in the order Co2+ < Zn2+ < Ni2+ < Mn2+ < Cu2+.
authors.affiliation: Graduate School of Engineering, Hokkaido University, Sapporo, Japan.
authors.forename: Tamio
authors.initials: T
authors.lastname: Kamidate
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tani
authors.forename: Akihiko
authors.initials: A
authors.lastname: Ishida