Application of Saccharomyces cerevisiae and Pichia stipitis karyoductants to the production of ethanol from xylose.
Karyoductants of Saccharomyces cerevisiae V30 and Pichia stipitis CCY 39501 with the ability to ferment D-xylose to ethanol were isolated. The ability of these isolates to assimilate different sugars, ethanol tolerance and ethanol production from D-xylose was investigated. Karyoductants didn't grow on starch, lactose and cellobiose, like S. cerevisiae, but showed good growth on xylose and L-arabinose, like P. stipitis. All isolates fermented xylose to ethanol slower than P. stipitis and with lower yields, 0.09 - 0.16 g/g. They secreted also about 3.4 - 7.1 g/dm3 of xylitol to the culture medium (P. stipitis only 0.06 g/dm3). The karyoductants showed an average tolerance to ethanol when compared with the parent strains and fermented glucose in the presence of 6% alcohol whereas parent strain S. cerevisiae and P. stipitis showed exogenic ethanol tolerance of 9% and 3%, respectively.
Kordowska-Wiater
M
M
Department of Food Technology and Storage, Agriculture University, Lublin, Poland.
Targoński
Z
Z
D001089:Arabinose / Q000378:metabolism; D002459:Cell Fusion; D002475:Cellobiose / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D007785:Lactose / Q000378:metabolism; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D014993:Xylitol / Q000096:biosynthesis; D014994:Xylose / Q000378:metabolism*
D002475:Cellobiose; D000431:Ethanol; D013213:Starch; D014994:Xylose; D001089:Arabinose; D007785:Lactose; D014993:Xylitol