The influence of the esterification of the 3'(2') hydroxyl group of tRNA on the hydrolysis by venom phosphodiesterase (Crotalus adamanteus) was studied. It was shown that this esterification does not change the rate of hydrolysis. At pH 7-8, no significant differences were observed either when the alpha amino group of the attached amino acid was free or blocked, or when the aminoacid contained a free carboxyl group or a second amino group (glutamic acid or lysine). Aminoacyl-AMP or oligopeptidyl-AMP can be prepared by this method.
Yot
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P
Service de Biochimie, Département de Biologie, Centre d'Etudes Nucléaires, Saclay 91, Gif-sur Yvette, France
Gueguen
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P
Chapeville
F
F
Hydrolyse des aminoacyl-tARN par la phosphodiesterase du venin.