title: Respiratory syncytial virus and chlamydia are not detectable by PCR in ongoing vernal keratoconjunctivitis.
abstract: Respiratory syncytial virus (RSV) and chlamydial infection may be pathogenetic factors in allergic diseases, perhaps also in ocular allergy. We analyzed the presence of RSV and chlamydial nucleic acids using reverse transcription (RT)-PCR and PCR, respectively, in conjunctival biopsies from patients with vernal keratoconjunctivitis (VKC) in order to determine whether these agents play a role in the maintenance of the disease. All biopsy samples were negative for both RSV (n = 15 for VKC and n = 10 for control subjects) and chlamydia (n = 8 for VKC and n = 7 for control subjects) homologous sequences. A direct association between RSV or chlamydial infection and ongoing inflammation in VKC could, therefore, not be confirmed.
authors.affiliation: Department of Clinical Sciences, Division of Ophthalmology, St. Erik's Hospital, Karolinska Institutet, S-112 82 Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Koulikovska
authors.forename: I
authors.initials: I
authors.lastname: van der Ploeg
authors.forename: B
authors.initials: B
authors.lastname: Herrmann
authors.forename: P G
authors.initials: PG
authors.lastname: Montan
mesh_terms: D000293:Adolescent; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D003228:Conjunctiva / Q000821:virology; D003233:Conjunctivitis, Allergic / Q000821:virology*; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000302:isolation & purification; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012367:RNA, Viral