title: Effect of preparation conditions on properties and permeability of chitosan-sodium hexametaphosphate capsules.
abstract: Capsules were obtained by interpolymer complexation between chitosan (polycation) and sodium hexametaphosphate (SMP, oligoanion). The effect of the preparation conditions on the capsule characteristics was evaluated. Specifically, the influence of variables such as pH, ionic strength, reagent concentration, and additives on the capsule permeability properties was investigated using dextran as a model permeant. The capsule membrane permeability was found to increase by decreasing the chitosan/SMP ratio as well as adding mannitol to the oligoanion recipient bath. Increasing the ionic strength or the pH of the initial chitosan solution was also found to enhance the membrane permeability, moving the membrane exclusion limit to higher values. Generally, the capsules prepared tinder all tested conditions had a relatively low permeability which rarely exceeded a molecular cut-off of 40 kD based on dextran standards. Furthermore, the diffusion rate showed a strong temporal dependence, indicating that the capsules prepared under various conditions exhibit different apparent pore size densities on the surface. The results indicated that, in order to obtain the desired capsule mass-transfer properties, the preparation conditions should be carefully considered and adjusted. Adding a polyol as well as low salt amount (less than 0.15%) is preferable as a means of modulating the diffusion characteristics, without disturbing the capsule mechanical stability.
authors.affiliation: Department of Chemistry, Swiss Federal Institute of Technology-Lausanne. nela.anguelova@epfl.ch
authors.forename: N
authors.initials: N
authors.lastname: Angelova
authors.forename: D
authors.initials: D
authors.lastname: Hunkeler
mesh_terms: D002214:Capsules / Q000138:chemical synthesis / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry*; D048271:Chitosan; D004058:Diffusion; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009994:Osmolar Concentration; D010316:Particle Size; D010539:Permeability; D010710:Phosphates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity
chemical_list: D002214:Capsules; D004337:Drug Carriers; D010710:Phosphates; D011108:Polymers; D002686:Chitin; D048271:Chitosan; C009285:sodium polymetaphosphate