Xylan deposition on secondary wall of Fagus crenata fiber.
Delignified and/or xylanase-treated secondary walls of Fagus crenata fibers were examined by field emission scanning electron microscopy. Microfibrils with a smooth surface were visible in the innermost surface of the differentiating fiber secondary wall. There was no ultrastructural difference between control and delignified sections, indicating that lignin deposition had not started in the innermost surface of the cell wall. There was no ultrastructural difference between control and xylanase-treated sections. Microfibrils on the outer part of the differentiating secondary wall surface had globular substances in delignified sections. These globular substances disappeared following xylanase treatment, indicating that these globules are xylan. The globular substances were not visible near the inner part of the differentiating secondary wall but gradually increased toward the outer part of the secondary wall, indicating that xylan penetrated into the cell wall and continuously accumulated on the microfibrils. Mature-fiber secondary walls were also examined by field emission scanning electron microscopy. Microfibrils were not apparent in the secondary wall in control specimens. Microfibrils with many globular substances were observed in the delignified specimens. Following xylanase treatment, the microfibrils had a smooth surface without any globules, indicating that the globular substance is xylan. These results suggest that cellulose microfibrils synthesized on the plasma membrane are released into the innermost surface of the secondary wall and coated with a thin layer of xylan. Successive deposition of xylan onto the cell wall increases the microfibril diameter. The large amounts of xylan that accumulated on microfibrils appear globular but are covered with lignin after they are deposited.
Awano
T
T
Laboratory of Plant Cell Structure, Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan. awanot@kais.kyoto-u.ac.jp
Takabe
K
K
Fujita
M
M
D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure*; D029964:Fagus / Q000166:cytology* / Q000648:ultrastructure*; D008031:Lignin / Q000032:analysis / Q000378:metabolism; D020894:Microfibrils / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009005:Monosaccharides / Q000032:analysis / Q000737:chemistry; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000378:metabolism
D009005:Monosaccharides; D014990:Xylans; D008031:Lignin; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase