[Construction and expression of APP(SWE) transgenic mice model].
OBJECTIVE
To prepare transgenic mice model of human Swedish mutation of amyloid precursor protein(APP(SWE)) gene.


METHODS
By pronuclear microinjection, human APP    (SWE) genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice. Integral zygotes were implanted into the oviducts of pseudopregnant female mice. The integration and expression of exogeneous genes in offsprings were detected by polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH) and reverse transcription-ploymerase chain reaction (RT-PCR) analysis.


RESULTS
The success rate of microinjection and the birth rate were 76.62% and 10.38%, respectively. There were 6 founders and the integration rate was 35.29%. The exogeneous gene was transferred to the third generation. Expression of human APP(SWE) gene was detected in the brain, heart and muscle but not detected in the liver and kidney of transgenic mice.


CONCLUSIONS
The transgenic mice model of human APP(SWE) gene has been prepared successfully.
Liu
Wei
W
Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South University, Changsha, Hunan, 410078 P. R. China. zhuyanszzx@sina.com
Lu
Guangxiu
G
D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D010957:Plasmids / Q000235:genetics
D016564:Amyloid beta-Protein Precursor