Nitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1.
Two relatively low-copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid-cured cells resulted in a significant intermediate nitrite accumulation as compared to wild-type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0-kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8-kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters.
Barak
Yoram
Y
Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, 76100, Rehovot, Israel.
Yarmus
Merav
M
Shapira
Roni
R
van Rijn
Jaap
J
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009573:Nitrites / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D010084:Oxidation-Reduction; D010230:Paracoccus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D009573:Nitrites