TCRBV CDR3 diversity of CD4+ and CD8+ T-lymphocytes in HIV-infected individuals.
TCRBV CDR3 repertoire diversity was analyzed in a cross-sectional study of HIV-infected individuals by CDR3 fingerprinting/spectratyping and single strand conformation polymorphism (SSCP). Most TCRBV families were detected in CD4+ cells of HIV-infected patients with CD4 counts ranging from 35 to 1103. In patients with CD4 counts >500, CD4+ TCRBV CDR3 fingerprinting profiles contained subtle variations with generally gaussian-distributed sizes. Lower CD4 counts coincided with more fragmented TCRBV CDR3 repertoires, containing dominant bands and bands missing from the CDR3 profiles. The CD8+ population of the same patients exhibited skewed CDR3 profiles of the majority of TCR BV families at CD4 counts >500. Irregularity of CD8+ CDR3 size distribution was most profound at low CD4 counts and suggested domination of the CD8+ TCRBV repertoire by a limited number of clones. Skewed patterns of CDR3 diversity probably reflect (oligo)clonal expansion of particular CD4+ and CD8+ cell populations during chronic infection with HIV. In addition, irregular CDR3 profiles of CD4+ and CD8+ at low CD4 counts suggest diminished TCR repertoire diversity, which may contribute to immunodeficiency.
Raaphorst
Frank M
FM
Department of Microbiology, University of Texas Health Science Center at San Antonio, 78284, USA.
Schelonka
Robert L
RL
Rusnak
Janice
J
Infante
Anthony J
AJ
Teale
Judy M
JM
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D016172:DNA Fingerprinting; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D014644:Genetic Variation; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000235:genetics*
D017260:Receptor-CD3 Complex, Antigen, T-Cell