abstract: A component with high molecular weight is prepared from commercial bovine insulin by gel filtration on Sephadex G 50. The substance has little biological and immunological activity in comparison to insulin. The activity is not changed by incubation with trypsin. It associates in 1 M CH(3)COOH and possesses a molecular weight of 28,500 as shown by ultracentrifugation. The ability of the component to precipitate with bovine insulin antibodies demonstrates that it contains immunologically active insulin-like proteins and is not a homogenous substance.
authors.affiliation: Zentralinstitut für Diabetes "Gerhardt Katsch", Bereich experimentelle Diabetesforschung, Karlsburg bei, Greifswald, DDR
authors.forename: H
authors.initials: H
authors.lastname: Zühlke
authors.forename: K -D.
authors.initials: K
authors.lastname: Kohnert
authors.forename: M
authors.initials: M
authors.lastname: Ziegler
authors.forename: S
authors.initials: S
authors.lastname: Knospe
authors.forename: J
authors.initials: J
authors.lastname: Behlke
vernacular_title: Fraktionierung handelsüblichen insulins. Gewinnung und eigenschaften einer hochmolekularen, insulin-ähnlichen komponente.