Photorespiration-dependent increases in phospho enolpyruvate carboxylase, isocitrate dehydrogenase and glutamate dehydrogenase in transformed tobacco plants deficient in ferredoxin-dependent glutamine-alpha-ketoglutarate aminotransferase.
The metabolic cross-talk associated with re-assimilation of photorespiratory NH4+ was analysed in transformed tobacco (Nicotiana tabacum L.) plants with low activities of ferredoxin-dependent glutamine-alpha-ketoglutarate aminotransferase (Fd-GOGAT; EC 1.4.7.1). Amounts of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco; EC 4.1.1.39) protein and Rubisco transcripts were similar in all lines whether photorespiration rates were low (4,000 microl l(-1) CO2) or high (air). Leaf sucrose, hexose and starch contents were similar in all lines. In contrast, there was evidence that anaplerotic carbon flow was stimulated in the transformed lines with less than 60% Fd-GOGAT, since phospho enolpyruvate carboxylase (PEPc) activity and (PEPc) protein were increased. A strong positive correlation between leaf PEPc activity and glutamine accumulation was observed, suggesting that the increase in PEPc was related to the accumulation of glutamine. A modest stimulation of total NADP-isocitrate dehydrogenase (ICDH; EC 1.1.1.42) activity was also observed in the transformed lines with less than 60% Fd-GOGAT. This was accompanied by increases in both the cytosolic ICDH and mitochondrial NAD-isocitrate dehydrogenases (IDH; EC 1.1.1.41). IDH protein was also increased in the transformed plants with low Fd-GOGAT, suggesting that both IDH and ICDH are involved in the production of carbon skeletons (and ultimately alpha-ketoglutarate) necessary for the re-assimilation of NH4+. In contrast, PEPc, ICDH and IDH transcripts were similar in all lines. The aminating (but not the de-aminating) activity of NAD(H)-glutamate dehydrogenase (NAD(H)-GDH; EC 1.4.1.2) was greatly increased in plants with less than 60% of Fd-GOGAT after transfer to air. The data confirm that NH4+ or glutamine are involved in signalling, leading to modified gene expression and enzyme activity required for enhanced production of the C skeletons, to accommodate increases in the assimilation of photorespiratory NH4+. In addition, we provide the first demonstration of a compensatory role for NAD(H)-GDH in stabilising the leaf glutamic acid pool when Fd-GOGAT becomes limiting.
Ferrario-Mery
Sylvie
S
Unité de Nutrition Azotée des Plantes, INRA de Versailles, Route de Saint Cyr, 78026 Versailles Cedex, France. ferrario@versailles.inra.fr
Hodges
Michael
M
Hirel
Bertrand
B
Foyer
Christine H
CH
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004798:Enzymes / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D010101:Oxygen Consumption / Q000502:physiology* / Q000528:radiation effects; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology / Q000528:radiation effects; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D015398:Signal Transduction; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000502:physiology
D004798:Enzymes; D007656:Ketoglutaric Acids; D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D018698:Glutamic Acid; D002244:Carbon; D007521:Isocitrate Dehydrogenase; D000594:Amino Acid Oxidoreductases; D005969:Glutamate Dehydrogenase; C042233:glutamate synthase (ferredoxin); D010730:Phosphoenolpyruvate Carboxylase; D012273:Ribulose-Bisphosphate Carboxylase