The experimental research of R-phycoerythrin subunits on cancer treatment: a new photosensitizer in PDT.
The mouse tumor cell S180 and human liver carcinoma cell SMC 7721 cells were first treated with R-PE and its subunits (alpha, beta, gamma subunits), then irradiated with Argon laser (496 nm, 28.8 J/cm2). Survival rate was measured by MTT method. In order to compare the phototoxicity in normal cells, the mouse marrow cells were treated with photofrin II and beta-subunit, irradiated with 45 J/cm2 of light; survival rate was also measured by MTT method. The result showed that R-PE subunits had better PDT effect on s180 cells than R-PE and lower phototoxicity in marrow cells than photofrin II. Flow cytometric analysis showed that PDT results in a growth inhibition and a G0-G1 cell cycle arrest in SMC 7721 cells. The tumor cells inhibited by PDT in vivo were morphologically observed by TEM, the tumor cell death was due to the occlusion of tumor blood vessels and inducement of cell programmed death in nuclei. Therefore, with the advantage in special fluorescence activity, low molecular weight, good light absorbent character and weak phototoxicity, R-PE subunit is an attractive option for improving the selectivity of PDT.
Huang
Bei
B
College of Life Science, University of Science & Technology of China, Hefei, China.
Wang
Guang-Ce
GC
Zeng
Chen-Kui
CK
Li
Zhen-gang
ZG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D017323:Dihematoporphyrin Ether / Q000627:therapeutic use; D007834:Lasers; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D010799:Phycoerythrin / Q000627:therapeutic use*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D017319:Photosensitizing Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D010799:Phycoerythrin; D017323:Dihematoporphyrin Ether; C022616:thiazolyl blue