[The anti-ischemic effect of O, O-acetyldaurisoline].
O, O-acetyldaurisoline(Adau) is a derivative of daurisoline (Dau). In cultured PC12 cells, Adau was found to inhibit K+, Bay K8644 and norepinephrine induced intracellular free calcium concentration increase. Adau was also shown to protect PC12 cells from hypoxia-reoxygenation injury with EC50 of 64.3 +/- 12.5 mumol.L-1 by MTT assay. Adau (2.5, 5 and 10 mg.kg-1, i.v.) administrated 30 min before ischemia in bilateral carotid artery occlusion and four-vessel occlusion rats, attenuated the increase of lipid peroxide content and the decrease of SOD activity. These results show that Adau have significant protective effects against ischemic injury.
Wang
T
T
Department of Pharmacology, China Pharmaceutical University, Nanjing 210009.
Mu
H
H
Liu
G
G
D000470:Alkaloids / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D006801:Humans; D007511:Ischemia / Q000517:prevention & control*; D008297:Male; D029583:Menispermaceae / Q000737:chemistry*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D000470:Alkaloids; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; C456959:O,O-acetyldaurisoline; C023968:daurisoline; D013482:Superoxide Dismutase; D002118:Calcium