title: Studies on the biosynthesis of epothilones: hydroxylation of Epo A and B to epothilones E and F.
abstract: When Sorangium cellulosum So ce90 is grown without XAD adsorber resin there is a steady state of epothilone A and B biosynthesis and hydroxylation of these products to epothilones E and F. This biotransformation at position C-21 of the thiazole ring is not restricted to producers of epothilones. It is carried out by a substrate induced monooxygenase. Epothilones E and F are further degraded by opening of the lactone ring by an esterase. Steps of degradation of different strains of S. cellulosum are compared.
authors.affiliation: GBF, Gesellschaft für Biotechnologische Forschung mbH, Abteilung Naturstoffbiologie, Braunschweig, Germany. kge@gbf.de
authors.forename: Klaus
authors.initials: K
authors.lastname: Gerth
authors.forename: Heinrich
authors.initials: H
authors.lastname: Steinmetz
authors.forename: Gerhard
authors.initials: G
authors.lastname: Höflel
authors.forename: Hans
authors.initials: H
authors.lastname: Reichenbach
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D001711:Biotransformation; D034261:Epothilones; D006900:Hydroxylation; D018942:Macrolides / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D034261:Epothilones; D018942:Macrolides; C093787:epothilone A; C093788:epothilone B