title: [Percutaneous testicular sperm aspiration and intracytoplasmic sperm injection in the treatment of severe male infertility].
abstract: OBJECTIVE
  To investigate the efficacy of intracytoplasmic sperm injection (ICSI) combined with percutaneous testicular sperm aspiration (PTSA) in the treatment of severe male infertility.
  
  
  METHODS
  From October 1998 to December 2000, 162 couples were enrolled, and ICSI and PTSA were adopted. Ovarian stimulation was achieved by the short protocol. All metaphase II (M II) oocytes were selected for ICSI.
  
  
  RESULTS
  1,517 M II oocytes were injected in 185 cycles, 990 fertilized (65.3%), and 152 embryo implanted. 54 couples achieved clinical pregnancy (35.5%).
  
  
  CONCLUSIONS
  PTSA combined with ICSI is a rapid, convenient, painless and effective approach for the treatment of severe male infertility.
authors.affiliation: Embryo Laboratory, Shanghai JiAi Genetics and In-Vitro Fertilization Institute, Shanghai 200011, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Han
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000628:therapy*; D001239:Inhalation; D008297:Male; D010063:Ovum / Q000502:physiology; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000166:cytology; D016896:Treatment Outcome