Arabidopsis glutathione-dependent formaldehyde dehydrogenase is an S-nitrosoglutathione reductase.
S-Nitrosoglutathione (GSNO), an adduct of nitric oxide (NO) with glutathione, is known as a biological NO reservoir. Heterologous expression in Escherichia coli of a cDNA encoding a glutathione-dependent formaldehyde dehydrogenase of Arabidopsis thaliana showed that the recombinant protein reduces GSNO. The identity of the cDNA was further confirmed by functional complementation of the hypersensitivity to GSNO of a yeast mutant with impaired GSNO metabolism. This is the first demonstration of a plant GSNO reductase, suggesting that plants possess the enzymatic pathway that modulates the bioactivity and toxicity of NO.
Sakamoto
Atsushi
A
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, 739-8526, Higashi-Hiroshima, Japan. ahkkao@hiroshima-u.ac.jp
Ueda
Manami
M
Morikawa
Hiromichi
H
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D005816:Genetic Complementation Test; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000378:metabolism* / Q000494:pharmacology; D012440:Saccharomyces / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D011994:Recombinant Proteins; D026422:S-Nitrosoglutathione; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases