Macrophage activation includes high intracellular myeloperoxidase activity.
Macrophages recovered from the peritoneum of mice, 48 h after concanavalin A administration, are primed and have a higher content of myeloperoxidase (MPO) than resident cells. The increase in MPO content is accompanied by an increased capability of macrophages generate hypochlorous acid and increased peroxidase activity. Contrary to the common sense, neutrophils is not the source of the MPO activity found in primed macrophages since macrophages recovered from mice treated with antigranulocyte antibody preserve the peroxidase activity. Given the broad spectrum of action of MPO, the preservation of MPO in primed macrophages might play a special role in the killing of pathogens and inflammation.
Rodrigues
Maria Rita
MR
Departamento de Análises Clínicas e Toxicológicas, Universidade de São Paulo, São Paulo, SP, CEP 05508-900, Brazil.
Rodriguez
Dunia
D
Russo
Momtchilo
M
Campa
Ana
A
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002457:Cell Extracts / Q000737:chemistry / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology; D006997:Hypochlorous Acid / Q000378:metabolism; D007424:Intracellular Fluid / Q000201:enzymology*; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000201:enzymology; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D002273:Carcinogens; D002457:Cell Extracts; D003208:Concanavalin A; D008165:Luminol; D006997:Hypochlorous Acid; D009195:Peroxidase; D013755:Tetradecanoylphorbol Acetate