Mamu-DQA1 allele and genotype frequencies in a randomly sampled breeding colony of rhesus macaques (Macaca mulatta).
We studied the allelic and genotypic distribution of the major histocompatibility class-II locus DQA1 observed in a random sample of Indian rhesus macaques (Macaca mulatta) from a major breeding facility in the United States. The DNA was isolated from whole blood samples collected between 1991 and 1994 from 65 Indian rhesus monkeys. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), which involves use of specific amplification of DQA1 exon 2 and subsequent restriction digestion of the 242-base pair fragment, was used to genotype the animals for the 20 known macaque (Mamu)-DQA1 alleles. Frequencies for four alleles (DQA1*240x, *2502, *2503 and *0102) differed significantly from those reported in a smaller sample of rhesus macaques from the German Primate Center. The modest genetic survey of Mamu-DQA1 genotypes presented here will be particularly useful in designing epidemiologic studies that investigate associations between immunogenetic background and disease susceptibility in macaque models of human disease.
Rolfs
B K
BK
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California at Davis, USA.
Lorenz
J G
JG
Wu
C C
CC
Lerche
N W
NW
Smith
D G
DG
D000483:Alleles; D000818:Animals; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D012494:Sampling Studies
D000949:Histocompatibility Antigens Class II; C092258:Mamu-DQA1 protein, Macaca mulatta