title: [Determination of 6 isoflavonoids in the hairy root cultures of Astragalus membranaceus by HPLC].
abstract: An accurate RP-HPLC method for the determination of 10-hydroxy-3, 9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan, formonenetin (7-hydroxy-4'-methoxyisoflavone), 8, 3'-dihydroxy-7, 4'-dimethoxy-isoflavone, 2'-hydroxy-3', 4'-dimethoxyisoflavan-7-O-glucopyranoside and alycosin (7,3'-dihydroxy-4'-methoxyisoflavone) in the hairy root cultures of Astragalus membranaceus was developed. Among them, 10-hydroxy-3, 9-dimethoxypeterocarpan, (3R) 8,2'-dihydroxy-7,4'-dimethoxyisoflavan and 8,3'-dihydroxy-7,4'-dimethoxyisoflavone were new compounds in Astragalus membranaceus. The analytical column was Nucleosil C18. The mobile phases were methanol-water = 3:2 and 1:1 (v/v). The detection wavelengths were 254 nm and 280 nm. Calibration graphs were rectilinear between 2.5 micrograms and 12.5 micrograms. The recoveries were between 96.47% and 103.33%. The relative standard deviations (RSDs) of measurement precision test were 2.57%-6.52%. The flavonoid contents in the hairy root cultures were between 0.0005% and 0.0065% (dry weight).
authors.affiliation: Laboratory of Biotechnology of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine and Pharmacy, Shanghai 200032.
authors.forename: Z
authors.initials: Z
authors.lastname: Zheng
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Hu
mesh_terms: D027885:Astragalus propinquus / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D046508:Culture Techniques; D007529:Isoflavones / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development
chemical_list: D007529:Isoflavones