Magnetite-alginate beads for purification of some starch degrading enzymes.
Starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. beta-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.
Teotia
Sunita
S
Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi.
Gupta
M N
MN
D000464:Alginates / Q000737:chemistry*; D000681:Amylases / Q000737:chemistry* / Q000302:isolation & purification*; D001234:Aspergillus niger / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D001671:Biochemistry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D052203:Ferrosoferric Oxide; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification; D027723:Ipomoea batatas / Q000201:enzymology; D007501:Iron / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013213:Starch / Q000378:metabolism; D001614:beta-Amylase / Q000302:isolation & purification
D000464:Alginates; D010087:Oxides; D013213:Starch; D007501:Iron; D000681:Amylases; D006026:Glycoside Hydrolases; D001614:beta-Amylase; D005087:Glucan 1,4-alpha-Glucosidase; C100181:pullulanase; D052203:Ferrosoferric Oxide