title: The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus.
abstract: To understand the molecular mechanisms intrinsic to reproductive organ development a cDNA microarray, fabricated from flower bud cDNA clones, was used to isolate genes, which are specifically expressed during the development of the anther and pistil in Lotus japonicus. Cluster analysis of the microarray data revealed 21 and 111 independent cDNA groups, which were specifically expressed in immature and mature anthers, respectively. RT-PCR was performed to provide a direct assessment of the accuracy and reproducibility of our approach. Confirmation of our results suggests that cDNA microarray technology is an effective tool for identification of novel reproductive organ-specific genes.
authors.affiliation: Laboratory of Plant Breeding, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Endo
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsubara
authors.forename: Takao
authors.initials: T
authors.lastname: Kokubun
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Takahata
authors.forename: Tohru
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
authors.forename: Taku
authors.initials: T
authors.lastname: Demura
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D016000:Cluster Analysis; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000502:physiology*; D000070116:Lotus / Q000235:genetics* / Q000254:growth & development; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D018514:Plant Structures / Q000502:physiology*; D015203:Reproducibility of Results; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary