Could ivermectin have a synergic effect with albendazole in hydatidosis therapy?
The efficacy of ivermectin (IVM) alone, albendazole (ABZ) alone and a combination of both (IVM + ABZ) against Echinococcus granulosus protoscolices was studied by means of in vitro incubation. The maximum protoscolicidal effect was detected when we used IVM+ABZ in combination. In this case, protoscolex viability dropped to 35% in comparison with 50% for IVM alone and 82.5% with ABZ alone 18 days post-incubation. Only the protoscolices incubated with IVM + ABZ did not develop into cysts following their inoculation into mice. The changes in ultrastructure induced in the protoscolices after the different drug incubations are also described. The incubation of cysts with IVM and IVM + ABZ shows that IVM + ABZ in combination is more effective than IVM alone as only when we incubated the cysts with IVM + ABZ did they completely lose their infectivity to mice. This occurred after only 10 days post-incubation. Cellular alterations were also more marked with IVM+ABZ incubations with: (1) the presence of residual bodies, (2) numerous lipids droplets and (3) vacuoles in the cytoplasm of cytons. However, the truncated microtriches and the nuclei remained unaltered after 10 days.
Casado
N
N
Parasitology Laboratory, Faculty of Pharmacy, Universidad de Alcala, Ctra. Madrid-Barcelona, Alcala de Henares, Madrid, Spain
Moreno
M J
MJ
Urrea-París
M A
MA
Rodríguez-Caabeiro
F
F
D015766:Albendazole / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000923:Anticestodal Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004443:Echinococcosis / Q000188:drug therapy* / Q000517:prevention & control; D004446:Echinococcus / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D007559:Ivermectin / Q000494:pharmacology / Q000627:therapeutic use*; D008018:Life Cycle Stages; D021261:Parasitic Sensitivity Tests; D012756:Sheep; D013997:Time Factors
D000923:Anticestodal Agents; D007559:Ivermectin; D015766:Albendazole