title: Utilizing luminometry for monitoring growth of Listeria monocytogenes in its liquid or gelified monocultures and cocultures with "acid-only" Lactococcus lactis.
abstract: The light output of a bioluminescent recombinant strain of Listeria monocytogenes increased parallel with its viable cell counts during the exponential phase of the growth both in aerobic monocultures and mixed cultures, but dropped significantly at the commencement of the stationary phase. Suppression of L. monocytogenes by a nisin-less strain of Lactococcus lactis occurred only as an early induction of the stationary state of the target organism. In low-salt cocultures, an inverse linear correlation was found between the logarithmic initial counts of lactic acid bacteria and the extent of growth of Listeria. Decrease of luminometric activity of the bioluminescent test organism indicate sensitively the transformation of the cells into metabolically less active stationary state as a stress-adaptive response to nutrient depletion, "metabolic crowding", or, inimical processes.
authors.affiliation: Department of Refrigeration and Livestock Products' Technology, Szent István University, Budapest, Hungary. huto@omega.kee.hu
authors.forename: József
authors.initials: J
authors.lastname: Farkas
authors.forename: Eva
authors.initials: E
authors.lastname: Andrássy
authors.forename: Judit
authors.initials: J
authors.lastname: Beczner
authors.forename: Ildikó
authors.initials: I
authors.lastname: Vidács
authors.forename: László
authors.initials: L
authors.lastname: Mészáros
mesh_terms: D000898:Antibiosis; D015169:Colony Count, Microbial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013294:Lactococcus lactis / Q000254:growth & development*; D008089:Listeria monocytogenes / Q000254:growth & development*; D008163:Luminescent Measurements; D013696:Temperature