The mechanism of the hemolytic activity of polyene antibiotics.
The kinetics of the filipin-, amphotericin B- and nystatin-induced hemolysis of human erythrocytes were investigated. Filipin-induced hemolysis is of the damage type. It is an all-or-none process, partly inhibited by Ca2+ or Ba2+ but not by Mg2+, Na+ or SO42-. The hemolytic activity of filipin is explained by the formation of large aggregates within the erythrocyte membrane in the form of large perforations, permeable to substances of low molecular weight as well as to macromolecules, including hemoglobin. In isotonic KCl solution, both amphotericin B and nystatin, at low concentrations, form smaller aggregates within the membranes. As a result, the permeability of the membranes to KCl increases and hemolysis occurs. However, the kinetics of the hemolysis induced by the two polyenes is complex. The process shows some features of the permeability type and some of the damage type. It is suggested that amphotericin B and nystatin may simultaneously form a number of transport systems, differing in their molecular organisation and hemolytic activity. Their participation in erythrocyte membrane permeability can be modified by small changes in membrane organisation and the chemical composition of the incubation medium. In isotonic solutions of divalent cation chlorides, and at higher antibiotic concentration, additional aggregates, allowing divalent cations to permeate, appear. These structures do not permit SO4(2-) to permeate.
Knopik-Skrocka
Agnieszka
A
Department of Cytology and Histology, A. Mickiewicz University, 61-701 Poznań, Fredry 10, Poland.
Bielawski
Józef
J
D000666:Amphotericin B / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002412:Cations / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects; D005372:Filipin / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007552:Isotonic Solutions; D009761:Nystatin / Q000494:pharmacology; D009994:Osmolar Concentration; D011090:Polyenes / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002412:Cations; D007552:Isotonic Solutions; D011090:Polyenes; D009761:Nystatin; D000666:Amphotericin B; D005372:Filipin