title: Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique.
abstract: METHODS
  Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO2). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemiahypoxia.
  
  
  RESULTS
  Both systems measured PO2 accurately in the tonometer, and there was excellent correlation between them (r(2) = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected.
  
  
  CONCLUSIONS
  Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.
authors.affiliation: Department of Critical Care Medicine, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA. ashaw@mdanderson.org
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Shaw
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Zach
authors.initials: Z
authors.lastname: Thomas
authors.forename: Craig W
authors.initials: CW
authors.lastname: Stevens
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D003627:Data Interpretation, Statistical; D005336:Fiber Optic Technology; D005453:Fluorescence; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D008962:Models, Theoretical; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D010101:Oxygen Consumption; D010313:Partial Pressure; D011048:Polarography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014065:Tonometry, Ocular
chemical_list: D010100:Oxygen