title: The role of superoxide anion in the regulation of epidermal growth factor or the expression and proliferation of its receptor in prostate cancer cell line PC3.
abstract: The purpose of this study was to investigate the role of superoxide anion(O2-) in the regulation of epidermal growth factor (EGF) or epidermal growth factor receptor (EGFR) expression and proliferation in the prostate cancer cell line PC3. Cell proliferation was tested by a 3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide (MTT) assay in the presence of O2-, EGF or their combination. Immunohistochemistry was carried out to assay the expression of EGF or EGFR. EGF or EGFR mRNA expression in the cells treated with O2- was examined by in situ hybridisation. The proliferation was significantly inhibited by O2- in a concentration-dependent manner ranging from 9 to 36 micromol/l nicotinamide adenine dinucleotide (NADH) combined with 2-8 micromol/l N-methylphenazonium methyl sulfate (PMS). The enhancement of proliferation induced by 5 ng/ml EGF was significantly overcome by O2-. Although O2- was not able to alter EGFR mRNA expression, O2- at the concentration of 18 micromol/l NADH and 4 micromol/l PMS reduced EGFR protein expression. O2- at the concentration of 18 micromol/l NADH and 4 micromol/l PMS can downregulate EGF and EGF mRNA expression.
authors.affiliation: Institute of Urology, 2nd Affiliated Hospital of Lanzhou Medical College, PR China. qixiangjie@hotmail.com
authors.forename: Xiangjie
authors.initials: X
authors.lastname: Qi
authors.forename: Yirong
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Wang
authors.forename: Guolan
authors.initials: G
authors.lastname: Duan
authors.forename: Dashan
authors.initials: D
authors.lastname: Qin
authors.forename: Shengjun
authors.initials: S
authors.lastname: Fu
authors.forename: Ganping
authors.initials: G
authors.lastname: Zhong
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000502:physiology*; D066246:ErbB Receptors / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013481:Superoxides
chemical_list: D013481:Superoxides; D004815:Epidermal Growth Factor; D066246:ErbB Receptors