Phage display particles expressing tumor-specific antigens induce preventive and therapeutic anti-tumor immunity in murine p815 model.
The efficacy of phage display particles expressing tumor antigen P1A35-43 in inducing protective and therapeutic anti-tumor immune responses were studied. A protective immune response against a lethal progressive P815 mastocytoma tumor cell challenge was established after subcutaneous injection of phage display particles. Furthermore, the vaccine suppressed growth of pre-existing tumors. Immunization with the hybrid phage particles elicited P1A(35-43) specific CTL responses and a Th1-dominated immune response with phage particle-specific secretion of IFN-gamma but not IL-4. Our results indicate that phage display particles might be a useful vaccine form for tumor-associated antigen epitopes in tumor immunotherapy.
Wu
Yuzhang
Y
The Institute of Immunology, The Third Medicine University, Shapingba District, Chongqing, China. a65428305@public.cta.cq.cn
Wan
Ying
Y
Bian
Jiang
J
Zhao
Jianping
J
Jia
ZhengCai
Z
Zhou
Liyun
L
Zhou
Wei
W
Tan
Yang
Y
D000818:Animals; D001483:Base Sequence; D002857:Chromium / Q000378:metabolism; D017931:DNA Primers; D007167:Immunotherapy; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000401:mortality / Q000628:therapy; D010446:Peptide Fragments / Q000276:immunology*; D019151:Peptide Library; D010957:Plasmids; D015996:Survival Rate; D018417:Th1 Cells / Q000276:immunology
D017931:DNA Primers; D010446:Peptide Fragments; D019151:Peptide Library; D002857:Chromium; D015847:Interleukin-4; D007371:Interferon-gamma