Induced expression of a temperature-sensitive leucine-rich repeat receptor-like protein kinase gene by hypersensitive cell death and wounding in tobacco plant carrying the N resistance gene.
A gene encoding a receptor-like protein kinase was isolated as the gene induced in the early period of N gene-dependent hypersensitive cell death in tobacco leaves. The kinase domain expressed as a glutathione S-transferase fusion protein was capable of autophosphorylation, indicating that this gene encodes an active protein kinase. A high level of the transcript accumulated before necrotic lesion formation in tobacco mosaic virus (TMV)-inoculated tobacco leaves carrying the N gene but it was low in a tobacco cultivar lacking the N gene. A small but reproducible increase in the transcript was found 1-2 h after a temperature shift from 30 degrees C to 20 degrees C even in healthy leaves, suggesting the gene expression is temperature sensitive. The gene was named WRK for wound-induced receptor-like protein kinase, because the transcript increased to a maximum within 15-30 min of wounding. In suspension cultured tobacco cells, an increase in the transcript was found 15 min after transfer to a new medium, but it was suppressed under high osmotic pressures. The wound-induced WRK accumulation was enhanced by cycloheximide treatment, but not by known defense signal compounds (salicylic acid, jasmonic acid, 1-aminocyclopropan-1-carboxylic acid and abscisic acid) and some plant hormones. Thus, WRK is a wound-inducible and temperature-sensitive protein kinase gene induced before hypersensitive cell death probably through unknown signaling pathways.
Ito
Naoko
N
Core Research of Evolutionary Science and Technology, 2-3 Surugadai, Kannda, Chiyoda-Ku, Tokyo, 101-0062, Japan.
Takabatake
Reona
R
Seo
Shigemi
S
Hiraga
Susumu
S
Mitsuhara
Ichiro
I
Ohashi
Yuko
Y
D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000598:Amino Acids, Cyclic / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D054883:Oxylipins; D010766:Phosphorylation; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics* / Q000821:virology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical; D013696:Temperature; D014026:Nicotiana / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000254:growth & development
D000598:Amino Acids, Cyclic; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C023863:1-aminocyclopropane-1-carboxylic acid; D012965:Sodium Chloride; C011006:jasmonic acid; D000040:Abscisic Acid; D003513:Cycloheximide; D011494:Protein Kinases; D020156:Salicylic Acid