Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection.
A rapid and sensitive HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5-HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liquid-liquid extraction of human plasma with dichloromethane coupled with reversed-phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra- and inter-assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters.
Bauer
Steffen
S
Institute of Clinical Pharmacology, University Medical Center Charité, Humboldt University Berlin, Berlin, Germany. steffen.bauer@charite.de
Störmer
Elke
E
Kaiser
Rolf
R
Tremblay
Pierre-Benoit
PB
Brockmöller
Jürgen
J
Roots
Ivar
I
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics; D017294:Ondansetron / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D000077526:Tropisetron
D007211:Indoles; D012702:Serotonin Antagonists; D017294:Ondansetron; D000077526:Tropisetron