Demonstration of four immunoassay formats using the array biosensor.
The ability of a fluorescence-based array biosensor to measure and quantify the binding of an antigen to an immobilized antibody has been demonstrated using the four different immunoassay formats: direct, competitive, displacement, and sandwich. A patterned array of antibodies specific for 2,4,6-trinitrotoluene (TNT) immobilized onto the surface of a planar waveguide and used to measure signals from different antigen concentrations simultaneously. For direct, competitive, and displacement assays, which are one-step assays, measurements were obtained in real time. Dose-response curves were calculated for all four assay formats, demonstrating the array biosensor's ability to quantify the amount of antigen present in solution.
Sapsford
Kim E
KE
Center for Bioresource Development, George Mason University, Fairfax, Virginia 22030-4444, USA.
Charles
Paul T
PT
Patterson
Charles H
CH
Ligler
Frances S
FS
D000906:Antibodies / Q000737:chemistry; D015374:Biosensing Techniques; D004785:Environmental Pollutants / Q000032:analysis; D005456:Fluorescent Dyes; D007118:Immunoassay / Q000295:instrumentation*; D014303:Trinitrotoluene / Q000032:analysis
D000906:Antibodies; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D014303:Trinitrotoluene