We have applied successfully the active enzyme analytical centrifugation method (A.E.C.) to the study of the well known pyruvate dehydrogenase complex (P.D.C.) in crude extracts from E. coli. It is not possible to study the complex in a spectrophotometer (through its dehydrogenase activity) due to NADH oxidase activities. The A.E.C. method, which overcomes this difficulty, can also be viewed as a refined spectrophotometric method.
Schmitt
B
B
Institut de Biologie Moléculaire, C.N.R.S., VII, 2, place Jussieu Paris 5ème, Paris, France
Kolb
H
H
Cohen
R
R
Utilisation de la centrifugation analytique pour la mesure, au sein d'un extrait brut d'E. coli, du coefficient de sédimentation du complexe de décarboxylation oxidative du pyruvate.