title: Relationships between trabecular bone remodeling and bone vascularization: a quantitative study.
abstract: Beside its well-known role in bone development, vascularization plays a major role in bone cell migration for bone remodeling and metastatic tumor invasion. However, the various techniques used to identify vessels in bone have never been tested for trabecular bone vessel quantification, whereas bone remodeling quantitative parameters are commonly assessed. In this context, we developed and compared various histological techniques used to visualize blood vessels in rat bone in order to quantify them. First, several products were tested by intracardiac infusion to opacify the bone vascular network. The best results were obtained using either an India ink-1% agarose solution or an India ink-saturated barium sulfate solution followed by X-ray microradiography. Second, to identify the types of vessels, we also performed histoenzymology and immunohistochemistry stainings. Neither alkaline phosphatase (for endothelial cells) nor adenosine triphosphatase (ATPase) stainings (for smooth muscle cells) provided a low enough background to allow for vessel identification and quantification. For immunohistochemistry, various specific vessel constituents were analyzed: laminin, smooth muscle cell alpha-actin, factor VIII, and lectin Griffonia simplifolia. Anti-laminin and anti-smooth muscle cell alpha-actin antibodies gave the best results for quantification. Third, after optimization of these techniques, we performed quantitative bone and vessel histomorphometry on two groups of 12 rats each, for which bone remodeling and vessel number and area parameters were measured. No statistical differences were observed between the two groups, confirming the reproducibility of our measurements. A significant relationship was found between vessel number and histodynamic parameters; that is, bone formation rate correlated positively with India ink-positive vessel area (p < 0.009, r2 = 0.54) and alpha-actin-positive vessel number (p < 0.05, r2 = 0.66). Furthermore, we report reproducible techniques for visualization and quantification of vessels in bone that also allowed for simultaneous conventional bone histomorphometry. This methodology should help researchers to better understand the functional and anatomical relationship between trabecular bone and its vascularization during normal or pathological processes.
authors.affiliation: Laboratoire de Biologie et de Biochimie du Tissu Osseux, Equipe Mixte INSERM 9901 Faculté de Médecine, Saint-Etienne, France.
authors.forename: O
authors.initials: O
authors.lastname: Barou
authors.forename: S
authors.initials: S
authors.lastname: Mekraldi
authors.forename: L
authors.initials: L
authors.lastname: Vico
authors.forename: G
authors.initials: G
authors.lastname: Boivin
authors.forename: C
authors.initials: C
authors.lastname: Alexandre
authors.forename: M H
authors.initials: MH
authors.lastname: Lafage-Proust
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D002244:Carbon; D004396:Coloring Agents; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000502:physiology*; D016610:Tissue Embedding
chemical_list: D004396:Coloring Agents; C028433:chinese ink; D002244:Carbon; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases