Multiple thiol-anchor capped DNA-gold nanoparticle conjugates.
We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.
Li
Zhi
Z
Northwestern University, Department of Chemistry and Institute for Nanotechnology, 2145 Sheridan Road, Evanston, IL 60208, USA.
Jin
Rongchao
R
Mirkin
Chad A
CA
Letsinger
Robert L
RL
D004247:DNA / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D010316:Particle Size; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013873:Thionucleotides / Q000737:chemistry
D017979:Gold Colloid; D013438:Sulfhydryl Compounds; D013873:Thionucleotides; D004247:DNA