Effect of Brn-3a deficiency on CGRP-immunoreactivity in the dorsal root ganglion.
Immunohistochemistry for calcitonin gene-related peptide (CGRP) was performed on the dorsal root ganglion (DRG) and spinal cord in wildtype and knockout mice for Brn-3a. CGRP-immunoreactive (-IR) neurons were abundant in the DRG of wildtype, heterozygous and knockout mice. Cell size analysis revealed that CGRP-IR neurons were of various sizes in wildtype and heterozygous mice. In the knockout mice, however, most of CGRP-IR neurons were small. In the spinal cord of knockout mice, the number of CGRP-IR fibers increased in the dorsal column but decreased in the deep part of the dorsal horn. The loss of Brn-3a may have different effects on CGRP-IR expression in small and large DRG neurons.
Ichikawa
Hiroyuki
H
Department of Oral Function and Anatomy, 2-5-1 Shikata-cho, Okayama 700-8225, Japan.
Yamaai
Tomoichiro
T
Mo
Zeqian
Z
Xiang
Mengqing
M
Sugimoto
Tomosada
T
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics*; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics*
D004268:DNA-Binding Proteins; C494189:Pou4f1 protein, mouse; D050818:Transcription Factor Brn-3; D050819:Transcription Factor Brn-3A; D014157:Transcription Factors; D015740:Calcitonin Gene-Related Peptide