A rapid cytokinin response assay in Arabidopsis indicates a role for phospholipase D in cytokinin signalling.
Seedlings of Arabidopsis thaliana harboring a fusion of the cytokinin-responsive ARR5 gene promoter and the GUS reporter gene were used for a pharmacological approach to study cytokinin signal transduction. The assay was shown to be rapid, sensitive, dose-dependent and highly specific for cytokinins, both adenine and phenylurea derivatives. Numerous inhibitors of known signalling pathways were tested and some were shown to suppress reporter gene induction. Particularly, primary alcohols that specifically inhibit phospholipase D (PLD) partially prevented cytokinin-induced GUS activity and reduced the accumulation of ARR5 gene transcripts. This indicates a role for PLD early during cytokinin signalling.
Romanov
Georgy A
GA
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276, Moscow, Russia. romanov@srvr.ippras.ru
Kieber
Joseph J
JJ
Schmülling
Thomas
T
D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000032:analysis / Q000378:metabolism; D001681:Biological Assay / Q000379:methods*; D003583:Cytokinins / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010944:Plants / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D012680:Sensitivity and Specificity; D015398:Signal Transduction / Q000502:physiology*; D015533:Transcriptional Activation
D029681:Arabidopsis Proteins; D003583:Cytokinins; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D018749:RNA, Plant; D010739:Phospholipase D; D005966:Glucuronidase