title: Multiple thiol-anchor capped DNA-gold nanoparticle conjugates.
abstract: We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.
authors.affiliation: Northwestern University, Department of Chemistry and Institute for Nanotechnology, 2145 Sheridan Road, Evanston, IL 60208, USA.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.forename: Rongchao
authors.initials: R
authors.lastname: Jin
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
authors.forename: Robert L
authors.initials: RL
authors.lastname: Letsinger
mesh_terms: D004247:DNA / Q000737:chemistry*; D017979:Gold Colloid / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D010316:Particle Size; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013873:Thionucleotides / Q000737:chemistry
chemical_list: D017979:Gold Colloid; D013438:Sulfhydryl Compounds; D013873:Thionucleotides; D004247:DNA