Evaluation of sorbitol-salicin MacConkey medium containing cefixime and tellurite (CT-SSMAC medium) for isolation of Escherichia coli O157:H7 from raw vegetables.
The utility of CT-SSMAC medium (sorbitol-salicin MacConkey medium containing cefixime and tellurite) for the isolation of Escherichia coli O157:H7 from raw vegetables was investigated. The colonies of all E. coli O157:H7 and O157:NM strains tested were colorless and beta-galactosidase-positive on CT-SSMAC medium. Furthermore, the number of colorless colonies on the CT-SSMAC medium was less than that on the sorbitol MacConkey medium containing cefixime and tellurite (CT-SMAC medium) from several raw vegetable samples. All colorless colonies grown on CT-SSMAC medium from raw vegetable samples were beta-galactosidase-negative. These findings suggest that the CT-SSMAC medium is useful for the isolation of E. coli O157:H7 from raw vegetable samples.
Fujisawa
Tomohiko
T
Kanagawa Prefectural Public Health Laboratory, Yokohama, Japan.
Sata
Shin
S
Aikawa
Katsuhiro
K
Takahashi
Takanori
T
Yamai
Shiro
S
Shimada
Toshio
T
D000362:Agar; D020682:Cefixime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D015169:Colony Count, Microbial / Q000379:methods*; D003116:Color; D003470:Culture Media; D019453:Escherichia coli O157 / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D013691:Tellurium / Q000494:pharmacology; D014675:Vegetables / Q000382:microbiology*; D001616:beta-Galactosidase / Q000378:metabolism
D002511:Cephalosporins; D003470:Culture Media; D000362:Agar; D020682:Cefixime; D001616:beta-Galactosidase; C026660:tellurous acid; D013691:Tellurium