title: Effects of dental resins on TNF-alpha-induced ICAM-1 expression in endothelial cells.
abstract: Many reports have demonstrated inflammation after the placement of dental restorations. To explain this side-effect, we studied a biomarker in the inflammatory response. The intercellular adhesion molecule-1 (ICAM-1) is a key mediator for recruitment of leukocytes to the site of inflammation. Therefore, we investigated whether methacrylates (a BISGMA-based dental resin, BISGMA, and MAA) and Cyracure UVR 6105, an epoxy monomer, could alter ICAM-1 expression in unstimulated and TNF-alpha-stimulated endothelial cells. Six-well plates with monolayers of human umbilical vein cells, ECV 304 (ATCC CRL 1998), were exposed to TNF-alpha (1 ng/mL) in the presence and absence of subtoxic and TC50 doses of chemicals for 24 hrs at 37 degrees C/5% CO2. Several doses of TNF-alpha (0.5-2 ng/mL) were coincubated with 100 microL of undiluted aqueous dental resin extracts. Cells were harvested and stained with mAB FITC-conjugated anti-human ICAM-1 (CD54). ICAM-1 expression was measured by flow cytometry. Cells expressed basal levels of ICAM-1, which was up-regulated by TNF-alpha but was not changed by all samples studied. Except for UVR 6105, the methacrylates significantly decreased ICAM-1 expression in TNF-alpha-stimulated cells. These findings suggest that methacrylates may decrease the recruitment of leukocytes to sites of inflammation.
authors.affiliation: University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 64108, USA.
authors.forename: E L
authors.initials: EL
authors.lastname: Kostoryz
authors.forename: P Y
authors.initials: PY
authors.lastname: Tong
authors.forename: A F
authors.initials: AF
authors.lastname: Strautman
authors.forename: A G
authors.initials: AG
authors.lastname: Glaros
authors.forename: J D
authors.initials: JD
authors.lastname: Eick
authors.forename: D M
authors.initials: DM
authors.lastname: Yourtee
mesh_terms: D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects; D003188:Composite Resins / Q000633:toxicity; D003509:Cyclohexanecarboxylic Acids / Q000633:toxicity; D003764:Dental Materials / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000187:drug effects; D008689:Methacrylates / Q000633:toxicity; D012117:Resins, Synthetic / Q000633:toxicity*; D012822:Silicon Dioxide / Q000633:toxicity; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015040:Zirconium / Q000633:toxicity
chemical_list: D003188:Composite Resins; D003509:Cyclohexanecarboxylic Acids; D003764:Dental Materials; D008689:Methacrylates; D012117:Resins, Synthetic; D014409:Tumor Necrosis Factor-alpha; C083568:Z100 composite resin; D018799:Intercellular Adhesion Molecule-1; C013353:EP 201; C008384:methacrylic acid; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D015040:Zirconium