Recurrent molecular deletion of the 12p13 region, centromeric to ETV6/TEL, in T-cell prolymphocytic leukemia.
BACKGROUND
T-cell prolymphocytic leukemia is a rare form of mature leukemia which occurs in adults and in younger patients suffering ataxia telangiectasia. Among others, complex chromosome aberrations of chromosome 12 have been described in this disease. We searched for deletions of the 12p13 region as the result of these chromosome rearrangements.


METHODS
Paired leukemic and non-leukemic cells were obtained from a series of 21 patients suffering T-cell prolymphocytic leukemia. Loss of heterozygosity was searched for by microsatellite typing using a fluorescent automated laser DNA sequencer to analyze the amplification products. Proteins were analyzed by Western blot. Southern blot analysis of one patient was conducted.


CONCLUSIONS
Loss of heterozygosity of the 12p13 region, including the ETV6 and CDKN1B genes, was detected in nine of these 21 cases (43%). Western and Southern blot analyses of one case demonstrated a biallelic deletion which did not include ETV6. Taken together, our results defined a minimal region of deletion of less than one Mb flanked by the markers b312C2T7 and D12S320, excluding ETV6 as a candidate gene. Deletion of the 12p13 region is thus a highly recurrent genetic event in T-cell prolymphocytic leukemia.
Hetet
G
G
Unité INSERM U409 and Centre de recherche Claude Bernard, Hôpital Bichat, Paris, France.
Dastot
H
H
Baens
M
M
Brizard
A
A
Sigaux
F
F
Grandchamp
B
B
Stern
M H
MH
D002503:Centromere / Q000235:genetics*; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D004273:DNA, Neoplasm / Q000097:blood / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D015463:Leukemia, Prolymphocytic / Q000235:genetics*; D015458:Leukemia, T-Cell / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D010750:Phosphoproteins / Q000235:genetics; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D000097764:ETS Translocation Variant 6 Protein
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D010750:Phosphoproteins; D050783:Proto-Oncogene Proteins c-ets; D012097:Repressor Proteins; D000097764:ETS Translocation Variant 6 Protein