The production of anti-hexapeptide antibodies which recognize the S7, L6 and L13 ribosomal proteins of Escherichia coli.
Here we report the synthesis of the N-terminal hexapeptide H-Pro-Arg-Arg-Arg-Val-Ile-OH of the E. coli ribosomal protein S7. the C-terminal hexapeptide H-Lys-Glu-Ala-Lys-Lys-Lys-OH of L6 and the C-terminal hexapeptide H-Pro-Gln-Val-Leu-Asp-Ile-OH of L13. All peptides were prepared by SPPS following the Fmoc-strategy, using DIC/HOBt and/or HBTU as coupling reagents and 2-chlorotrityl chloride resin as the solid support. The carrier linked synthetic peptides were injected into rabbits and elicited an anti-peptide response. These anti-hexapeptide antibodies were found to recognize the corresponding peptides and proteins.
Metaxas
Aristomenis
A
Department of Chemical Engineering, Aristotle University of Thessaloniki, Greece.
Tzartos
Socrates
S
Liakopoulou-Kyriakide
Maria
M
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism*; D015151:Immunoblotting; D013058:Mass Spectrometry; D010047:Ovalbumin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012269:Ribosomal Proteins / Q000737:chemistry*
D000906:Antibodies; D010455:Peptides; D012269:Ribosomal Proteins; C031249:ribosomal protein L6; C037442:ribosomal protein S7; D010047:Ovalbumin