Specific immune responses in changed gaseous environments.
The capacity of lymphoid cells to participate in immunity reactions was evaluated by blast transformation of lymphocytes under the influence of phytohemagglutinin. Blast transformation was measured by cytologic analysis and autoradiographic investigation of the rate of RNA synthesis in cells (tritiated uridin used as label). An analysis of the material taken from the three test subjects during the year-long experiment showed that various situations affected significantly the blast transformation level of lymphocytes. The reaction was substantially reduced 10 days after a simulated emergency situation which involved a change in the atmosphere, increase of physical load, etc. The level of blast transformation increased 1.5 to 2 months after the simulation, exceeding the average value, then to be normalized. Atmospheric variations appear to be one of the factors that may change the activity of lymphoid cells. A parallel experiment was performed in which three subjects lived 10 days in a hyperoxic enclosed environment (53% O2). They showed a considerable intensification of blast transformation (by 2.2-2.6 times) and pronounced activation of the RNA synthesis. Investigations give evidence that a long-term enclosure exerts an effect on the reactivity of the systems involved in the development of basic immune reactions.
Konstantinova
I V
IV
Institute of Biomedical Problems, USSR Ministry of Public Health, Moscow, USSR.
Lebedev
K A
KA
Zemskov
V M
VM
Zazhirey
V D
VD
Ganina
V I
VI
D000389:Air Conditioning; D000818:Animals; D001272:Atmosphere / Q000737:chemistry*; D002245:Carbon Dioxide / Q000032:analysis / Q000494:pharmacology*; D004168:Diphtheria Toxoid / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D018504:Ecological Systems, Closed; D006388:Hemagglutinins / Q000097:blood / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D007121:Immunocompetence / Q000187:drug effects* / Q000276:immunology; D008021:Life Support Systems; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D010100:Oxygen / Q000032:analysis / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D004168:Diphtheria Toxoid; D006388:Hemagglutinins; D010835:Phytohemagglutinins; D002245:Carbon Dioxide; D010100:Oxygen