The angiogenic factor heparin affin regulatory peptide (HARP) induces proliferation of human peripheral blood mononuclear cells.
Heparin affin regulatory peptide (HARP) also named pleiotrophin (PTN) is a polypeptide that belongs to a family of heparin-binding molecules. HARP displays mitogenic activity for a wide variety of cells, including fibroblast, endothelial and epithelial cells. This study reports, to our knowledge for the first time that HARP induced the stimulation of triated thymidine incorporation in quiescent human peripheral blood mononuclear cells in a dose-dependant manner, measured after 7 days of culture. Maximal stimulation was observed at picomolar concentration with ED50 corresponding to the half maximum effect at 50 pM. In contrast, midkine (MK), a related heparin-binding growth/differentiation factor, with more than 50% amino acid sequence homology with HARP was ineffective. These results suggest that HARP could be considered as a new cytokine involved inthe growth regulation of cell mediated immunity.
Achour
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Laboratoire de Recherche sur la Croissance Cellulaire, la Reparation et la Regénération Tissulaires (CRRET), CNRS UPRES-A 7053, Université Paris Val de Marne, Créteil, France.
Laaroubi
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Caruelle
D
D
Barritault
D
D
Courty
J
J
D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites; D002352:Carrier Proteins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D000078792:Midkine; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D043925:Angiogenesis Inducing Agents; D002352:Carrier Proteins; D016207:Cytokines; D006133:Growth Substances; D011994:Recombinant Proteins; C066845:pleiotrophin; D000078792:Midkine; D013936:Thymidine