title: Adenovirus infection targets the cellular protein kinase CK2 and RNA-activated protein kinase (PKR) into viral inclusions of the cell nucleus.
abstract: The effects of the adenovirus infection on the distribution of the cellular protein kinase CK2 and double-stranded RNA-activated protein kinase (PKR) were examined at the ultrastructural level. Immunogold labeling revealed the redistribution of CK2 subunits and PKR to morphologically distinct structures of the cell nucleus. The electron-clear amorphous structures, designated pIX nuclear bodies in our previous work (Rosa-Calatrava et al., 2001), contained CK2 alpha and PKR. The protein crystals, which result from the regular assembly of hexon, penton base, and fiber proteins [Boulanger et al. (1970) J Gen Virol 6:329-332], contained CK2 beta and PKR. Both viral structures were devoid of viral RNA, including the PKR-inhibitor VA1 RNA generated by the RNA polymerase III. Instead, VA1 RNA accumulated in PKR-free viral compact rings in which the viral RNA generated by the RNA polymerase II was excluded.
authors.affiliation: Institut André Lwoff, UPR 1983 CNRS, Villejuif, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Souquere-Besse
authors.forename: Evelyne
authors.initials: E
authors.lastname: Pichard
authors.forename: Odile
authors.initials: O
authors.lastname: Filhol
authors.forename: Valerie
authors.initials: V
authors.lastname: Legrand
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
authors.forename: Ara G
authors.initials: AG
authors.lastname: Hovanessian
authors.forename: Claude
authors.initials: C
authors.lastname: Cochet
authors.forename: Francine
authors.initials: F
authors.lastname: Puvion-Dutilleul
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D047390:Casein Kinase II; D002467:Cell Nucleus / Q000648:ultrastructure*; D006367:HeLa Cells / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D007181:Inclusion Bodies, Viral / Q000201:enzymology*; D008854:Microscopy, Electron; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D019892:eIF-2 Kinase / Q000378:metabolism*
chemical_list: D012330:RNA, Double-Stranded; D012335:RNA, Ribosomal; D012367:RNA, Viral; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D019892:eIF-2 Kinase