title: Contribution of hemolysin and phospholipase activity to cytolytic properties and viability of Listeria monocytogenes.
abstract: The life cycle of Listeria monocytogenes strongly depends on cytolysin which facilitates the pathogen's escape from the endosome. This process is very ineffective in the absence of phospholipases. In this study we investigated the molecular mechanism of cooperation between hemolysin and phospholipases. We propose a model involving interactions between proteins that increase the affinity of cytolysins for the membrane as well direct enzymatic enhancement of lysis.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Warsaw University, Poland. radeks@biol.uw.edu.pl
authors.forename: R
authors.initials: R
authors.lastname: Stachowiak
authors.forename: J
authors.initials: J
authors.lastname: Bielecki
mesh_terms: D001427:Bacterial Toxins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006460:Hemolysin Proteins; D006461:Hemolysis / Q000502:physiology; D008089:Listeria monocytogenes / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010740:Phospholipases / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D010740:Phospholipases; C038705:hlyA protein, Listeria monocytogenes