title: Use of at-line spectrophotometry for the rapid definition of pilot-scale flocculation processes.
abstract: Traditionally most downstream bioprocesses have been operated without real-time knowledge of product and key contaminants, yielding little confidence in their operation and the impact on subsequent operations. A rapid UV-vis spectral prediction technique has been successfully demonstrated for the at-line characterization of a large scale continuous flocculation process in terms of RNA, key protein contaminants, and cell debris. A comparison was made between the spectral predictions and retrospective wet chemical assays, and a highly linear correlation was obtained. The spectral analysis technique allowed for real-time system information, which was applied to control the flocculation process to maintain satisfactory process performance, even when subjected to given possible process disturbances.
authors.affiliation: The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, United Kingdom.
authors.forename: G
authors.initials: G
authors.lastname: Bou-Habib
authors.forename: K
authors.initials: K
authors.lastname: Yeung
authors.forename: N J
authors.initials: NJ
authors.lastname: Titchener-Hooker
authors.forename: M
authors.initials: M
authors.lastname: Hoare
mesh_terms: D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000302:isolation & purification; D000818:Animals; D002138:Calibration; D002417:Cattle; D002498:Centrifugation; D005240:Feasibility Studies; D005246:Feedback; D005427:Flocculation; D015233:Models, Statistical; D015999:Multivariate Analysis; D010865:Pilot Projects; D012313:RNA / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013347:Subcellular Fractions
chemical_list: D012710:Serum Albumin, Bovine; D012313:RNA; D000426:Alcohol Dehydrogenase