title: Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.
abstract: Mutations arising in Ig V genes during an immune response are most likely introduced by one or several error-prone DNA polymerases. Many of the recently described nonreplicative DNA polymerases have an intrinsic fidelity compatible with such an activity, the strongest candidates being polymerase (pol) eta, pol iota, pol zeta, and pol mu. We report in this work that mice inactivated for either of the two polymerases related to pol beta (i.e., pol mu and pol lambda) are viable and fertile and display a normal hypermutation pattern.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 373, Faculté de Médecine Necker-Enfants Malades, Paris, France.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bertocci
authors.forename: Annie
authors.initials: A
authors.lastname: De Smet
authors.forename: Eric
authors.initials: E
authors.lastname: Flatter
authors.forename: Auriel
authors.initials: A
authors.lastname: Dahan
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Bories
authors.forename: Catherine
authors.initials: C
authors.lastname: Landreau
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Weill
authors.forename: Claude-Agnès
authors.initials: CA
authors.lastname: Reynaud
mesh_terms: D000818:Animals; D019951:DNA Polymerase beta / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D004259:DNA-Directed DNA Polymerase / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D020868:Gene Silencing / Q000276:immunology; D005803:Genes, Immunoglobulin / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation / Q000276:immunology*
chemical_list: C415367:DNA polymerase beta2; C407743:DNA polymerase mu; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase