Synergistic effect of interleukin-2 and a vaccine of irradiated melanoma cells transfected to secrete staphylococcal enterotoxin A.
We have previously reported that immunization of mice with melanoma cells transfected to secrete the superantigen, Staphylococcal enterotoxin A (SEA), increased the production of antibodies to the B700 melanoma antigen, stimulated the production of endogenous interleukin 2 (IL-2), activated the expression of CD4, CD8 and CD25 T cell markers and enhanced NK cell activity. Now we show that immunization of mice with a vaccine of irradiated sea-transfected melanoma cells coupled with IL-2 therapy was even more effective in inhibiting the growth of primary melanoma tumors and the development of lung metastases than was the irradiated melanoma cell vaccine alone or IL-2 alone. The morphological and immunological effectiveness of the therapy was dose-dependent on IL-2.
Schrayer
David P
DP
Department of Surgery and Pathology, University Medical Group/Roger Williams Medical Center, Boston University School of Medicine, Providence, Rhode Island 02908, USA. David_Shrayer@Brown.edu
Kouttab
Nicola
N
Hearing
Vincent J
VJ
Wanebo
Harold J
HJ
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines / Q000494:pharmacology*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D004357:Drug Synergism; D004768:Enterotoxins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007114:Immunization; D007376:Interleukin-2 / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000556:secondary; D008545:Melanoma / Q000276:immunology* / Q000378:metabolism; D008546:Melanoma, Experimental / Q000556:secondary / Q000628:therapy*; D058950:Melanoma-Specific Antigens; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000276:immunology; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013552:Swine / Q000097:blood; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000528:radiation effects
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D004768:Enterotoxins; D007376:Interleukin-2; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine; C026066:enterotoxin A, Staphylococcal