title: [Lung transplantation and mitochondrial function].
abstract: The mechanisms of cellular lesions induced by lung ischemia and reperfusion are not fully understood and, in particular, the consequences of pulmonary ischemia and reperfusion injury on mitochondrial function have not been previously investigated. Therefore, we studied the respiratory function of isolated pulmonary mitochondria in a swine model of lung ischemia and reperfusion. We demonstrated that prolonged hypothermic (4 degrees C) ischemia induces significant lesions of the mitochondrial respiratory chain, particularly if ischemia is followed by normothermic reperfusion. These results should be integrated in the cellular alterations induced by the ischemia-reperfusion injury. In another swine model mimicking controlled non-heart beating donors, we demonstrated that thirty minutes of cardiac arrest do not promote significant alteration of the mitochondrial respiratory function. In contrast, forty-five minutes of cardiac arrest, induced significant mitochondrial lesions. This pulmonary tolerance to normothermic cardiac arrest might be explained by the presence of air in the lung airways, allowing some aerobic metabolism after circulatory arrest. These results suggested that lung grafts might be harvested from non-heart beating donors after thirty minutes of cardiac arrest, significantly increasing the pulmonary graft pool.
authors.affiliation: Service de Chirurgie, de Transplantation et de Bioénergétique, Centre de recherches du département de Chirurgie (CREDEC), Université de Liège au Sart-Tilman.
authors.forename: O
authors.initials: O
authors.lastname: Detry
authors.forename: K
authors.initials: K
authors.lastname: Willet
authors.forename: M
authors.initials: M
authors.lastname: Meurisse
authors.forename: I
authors.initials: I
authors.lastname: Pincemail
authors.forename: R
authors.initials: R
authors.lastname: Limet
authors.forename: F E
authors.initials: FE
authors.lastname: Sluse
authors.forename: J O
authors.initials: JO
authors.lastname: Defraigne
mesh_terms: D000818:Animals; D016040:Lung Transplantation; D008928:Mitochondria / Q000502:physiology*
vernacular_title: Transplantation pulmonaire et fonction mitochondriale.