Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis.
A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells.
Senoo
Makoto
M
Department of Immunology, Tokai University School of Medicine, Bouseidai, Isehara, Kanagawa, 259-1193, Japan.
Hoshino
Shigetaka
S
Mochida
Naoko
N
Matsumura
Yasuko
Y
Habu
Sonoko
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D003456:Cryptorchidism / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006358:Hot Temperature; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
D011506:Proteins; D012333:RNA, Messenger; C486737:SPATS2 protein, human; C454107:Spats2 protein, mouse; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081931:MAK protein, human; C509962:Mak protein, mouse