title: Association study between a novel single nucleotide polymorphism of the promoter region of the prostacyclin synthase gene and essential hypertension.
abstract: The purpose of this study was to investigate whether an association exists between the promoter region of the prostacyclin synthase gene and essential hypertension (EH). Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, we discovered a novel single nucleotide polymorphism (SNP), T-192G, in the 5'-flanking region. We performed an association study using the SNP in 200 patients and 200 controls. The allele frequency distribution in the two groups was not significantly different. Thus, this SNP in the PGIS gene is not associated with EH.
authors.affiliation: Division of Receptor Biology, Advanced Medical Research Center, Tokyo, Japan. tnakayam@med.nihon-u.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakayama
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Soma
authors.forename: Dolkun
authors.initials: D
authors.lastname: Rahmutula
authors.forename: Hideko
authors.initials: H
authors.lastname: Tobe
authors.forename: Mikano
authors.initials: M
authors.lastname: Sato
authors.forename: Jiro
authors.initials: J
authors.lastname: Uwabo
authors.forename: Noriko
authors.initials: N
authors.lastname: Aoi
authors.forename: Kotoko
authors.initials: K
authors.lastname: Kosuge
authors.forename: Masako
authors.initials: M
authors.lastname: Kunimoto
authors.forename: Katsuo
authors.initials: K
authors.lastname: Kanmatsuse
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kokubun
mesh_terms: D000328:Adult; D000483:Alleles; D001483:Base Sequence / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012016:Reference Values; D020080:Tandem Repeat Sequences
chemical_list: D003577:Cytochrome P-450 Enzyme System; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase