title: Adipose tissue: a vital in vivo role in mammary gland development but not differentiation.
abstract: Development and differentiation of the mammary gland occurs by means of critical stromal-epithelial interactions. Although many studies have attempted to understand these complex interactions, it has been difficult to demonstrate the essential role of adipose tissue in the development and function of the mammary gland. By using the A-ZIP/F-1 transgenic mice lacking in white adipose tissue (WAT), we have studied the role of adipocytes in mammary gland development and differentiation. In the absence of WAT, rudimentary mammary anlagen form but are unable to grow and branch normally, resulting in a few, short, severely distended ducts. However, during pregnancy, a tremendous amount of epithelial cell division and alveolar cell formation occurs even in the absence of adipocytes, illustrating that adipose tissue is not required for mammary gland differentiation. Mammary gland transplantation revealed that epithelial cells from these transgenic mice possess the potential for normal growth and differentiation when placed into a normal stromal environment. These experiments clearly demonstrate that the absence of adipocytes in the mammary gland results in disruption of stromal-epithelial interactions that prevent normal mammary gland development. The rudimentary epithelial anlage, however, contain mammary stem cells, which are fully capable of alveolar differentiation.
authors.affiliation: Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, Bethesda, Maryland 29896, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Couldrey
authors.forename: Jaideep
authors.initials: J
authors.lastname: Moitra
authors.forename: Charles
authors.initials: C
authors.lastname: Vinson
authors.forename: Miriam
authors.initials: M
authors.lastname: Anver
authors.forename: Kunio
authors.initials: K
authors.lastname: Nagashima
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Green
mesh_terms: D017667:Adipocytes; D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D001940:Breast / Q000166:cytology / Q000254:growth & development*; D002454:Cell Differentiation; D002455:Cell Division; D004847:Epithelial Cells; D004958:Estradiol / Q000097:blood; D005260:Female; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis
chemical_list: D004958:Estradiol