title: Immunoproteomics of Helicobacter pylori infection and relation to gastric disease.
abstract: The Gram negative bacterium Helicobacter pylori is a human pathogen which infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. A systematic, proteome based approach was chosen to detect candidate antigens of H. pylori for diagnosis, therapy and vaccine development and to investigate potential associations between specific immune responses and manifestations of disease. Sera from patients with active H. pylori infection (n = 24), a control group with unrelated gastric disorders (n = 12) and from patients with gastric cancer (n = 6) were collected and analyzed for the reactivity against proteins of the strain HP 26695 separated by two-dimensional electrophoresis. Overall, 310 antigenic protein species were recognized by H. pylori positive sera representing about 17% of all spots separated. Out of the 32 antigens most frequently recognized by H. pylori positive sera, nine were newly identified and 23 were confirmed from other studies. Three newly identified antigens which belong to the 150 most abundant protein species of H. pylori, were specifically recognized by H. pylori positive sera: the predicted coding region HP0231, serine protease HtrA (HP1019) and Cag3 (HP0522). Other antigens were recognized differently by sera from gastritis and ulcer patients, which may identify them as candidate indicators for clinical manifestations. The data from these immunoproteomic analyses are added to our public database (http://www.mpiib-berlin.mpg.de/2D-PAGE). This platform enables one to compile many protein profiles and to integrate data from other studies, an approach which will greatly assist the search for more immunogenic proteins for diagnostic assays and vaccine design.
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany.
authors.forename: Gaby
authors.initials: G
authors.lastname: Haas
authors.forename: Galip
authors.initials: G
authors.lastname: Karaali
authors.forename: Karl
authors.initials: K
authors.lastname: Ebermayer
authors.forename: Wolfram G
authors.initials: WG
authors.lastname: Metzger
authors.forename: Stephanie
authors.initials: S
authors.lastname: Lamer
authors.forename: Ursula
authors.initials: U
authors.lastname: Zimny-Arndt
authors.forename: Susanne
authors.initials: S
authors.lastname: Diescher
authors.forename: Ulf B
authors.initials: UB
authors.lastname: Goebel
authors.forename: Konstanze
authors.initials: K
authors.lastname: Vogt
authors.forename: Artur B
authors.initials: AB
authors.lastname: Roznowski
authors.forename: Bertram J
authors.initials: BJ
authors.lastname: Wiedenmann
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Meyer
authors.forename: Toni
authors.initials: T
authors.lastname: Aebischer
authors.forename: Peter R
authors.initials: PR
authors.lastname: Jungblut
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D010449:Peptide Mapping; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013272:Stomach Diseases / Q000276:immunology* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D020543:Proteome