Quantitative FISH analysis on interphase nuclei may improve diagnosis of DNA diploid breast cancers.
The detection of DNA aneuploid cells using flow cytometry is an indication for the presence of tumor cells, but when DNA diploid cells are found in 25-33% of the cases, the diagnostic and prognostic significance of DNA ploidy is more limited. We analyzed interphase nuclei after in situ hybridization and using image cytometry on 50 breast tumors with diploid DNA content to investigate whether early chromosome rearrangements were detectable and if their occurrence was clinically significant. Imbalances between the two arms of chromosome 1 were found in 55% of the cases and values ranged from 1.5-3.0. Comparison with histological data showed that Grade I tumors mainly have imbalances (67%) and that Grade III tumors were mainly without the imbalance (67%), whereas Grade II tumors were intermediate (50% imbalance). These data suggest that the diagnosis of DNA diploid cases may be improved by using interphase FISH. In addition, the data also indicates that early breast tumors may have different genetic origins, which is important in the comprehension of tumor malignancy in early stages, especially for preinvasive lesions.
Truong
Khuong
K
Cytogénétique moléculaire et Oncologie, Unité Mixte de Recherche 147, Centre National de Recherche Scientifique-Institut Curie, Paris, France. khuong.truong@curie.fr
Vielh
Philippe
P
Guilly
Marie-Nöelle
MN
Klijanienko
Jerzy
J
Sastre-Garau
Xavier
X
Soussaline
Françoise
F
Dutrillaux
Bernard
B
Malfoy
Bernard
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000473:pathology; D018275:Carcinoma, Lobular / Q000235:genetics / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology*; D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D006801:Humans; D019044:Image Cytometry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008875:Middle Aged; D009367:Neoplasm Staging; D013544:Sweat Gland Neoplasms / Q000235:genetics / Q000473:pathology
D004273:DNA, Neoplasm