title: Sphaeropsidins D and E, two other pimarane diterpenes, produced in vitro by the plant pathogenic fungus Sphaeropsis sapinea f. sp. cupressi.
abstract: Two pimarane diterpenes structurally related to sphaeropsidins were isolated from the liquid culture of Sphaeropsis sapinea f. sp. cupressi, a plant pathogenic fungus causing a form of canker disease of Italian cypress (Cupressus sempervirens L.). The two metabolites, characterised by spectroscopic methods, were named sphaeropsidins D (0.40 mg l(-1)) and E (0.16 mg l(-1)). The same fungus produced sphaeropsidins A, B and C, sphaeropsidone and episphaeropsidone, which proved to be phytotoxic to cypress, and chlorosphaeropsidone and epichlorosphaeropsidone showing no phytotoxicity. Sphaeropsidin D assayed at 0.1 mg ml(-1) on severed cypress twigs caused leaf browning and necrosis on Cupressus macrocarpa, but no symptoms were observed on C. sempervirens and C. arizonica. Symptoms appeared in a period of time (6 days after toxin-treatment) shorter than that for sphaeropsidin A. Sphaeropsidin E assayed at 0.2 mg ml(-1) did not produce any symptom on the same cypress species tested with sphaeropsidin D.
authors.affiliation: Dipartimento di Scienze Chimico-Agrarie, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy. evidente@unina.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Evidente
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Sparapano
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bruno
authors.forename: Andrea
authors.initials: A
authors.lastname: Motta
mesh_terms: D001681:Biological Assay; D046508:Culture Techniques; D029781:Cupressus / Q000187:drug effects / Q000502:physiology; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000737:chemistry*; D010935:Plant Diseases / Q000382:microbiology; D018675:Toxicity Tests
chemical_list: D004224:Diterpenes; C465318:sphaeropsidin D; C465319:sphaeropsidin E