Nucleotide sequence of BamHI family satellite DNA and its unit length polymorphism in bluegill sunfish Lepomis macrochirus.
We have isolated and sequenced a member of tandem repetitive DNA containing BamHI site (BamHI family satellite DNA) from bluegill sunfish Lepomis macrochirus. PCR amplification with specific primers was performed to define the size of unit length repeat of the BamHI family satellite DNA, revealing that there were two distinct size of DNA fragments (0.9 kb and 1.3 kb) in the PCR products. The longer fragment (1.3 kb) consisted of internal sub-duplication of shorter fragment (0.9 kb). We have compared the size of PCR products among four fish populations, and found that both fragments co-existed in one population whereas the longer fragment was dominant in other three populations. The results may reflect ongoing homogenization of satellite DNA type over a short evolutionary time scale.
Takahashi
T
T
Department of Life Sciences, College of Integrated Arts and Sciences, Osaka Prefecture University, Sakai, Japan.
Kawamura
Y
Y
Sakata
N
N
Elmesiry
G E
GE
Takemon
Y
Y
Tanida
K
K
Minoshima
S
S
Shimizu
N
N
Kato
M
M
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004276:DNA, Satellite / Q000235:genetics*; D015245:Deoxyribonuclease BamHI; D005828:Genetics, Population; D007564:Japan; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D004276:DNA, Satellite; D015245:Deoxyribonuclease BamHI