title: Effect of immobilization stress on brain polyamine levels in spontaneously hypertensive and Wistar-Kyoto rats.
abstract: The present study aimed to compare the basal brain polyamine levels and stress-induced brain polyamine level changes in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. For immobilization stress, both strains underwent acute (3 h per day immobilization for 2 days), chronic (3 h per day immobilization for 15 consecutive days), or no immobilization stress (control group). Basal putrescine (PU) levels in frontal cortex and hippocampus of SHR (11.03 +/- 0.81 and 11.36 +/- 0.33 nmol/g tissue, respectively) were significantly higher than WKY rats (6.90 +/- 1.44 and 7.82 +/- 0.71 nmol/g tissue, respectively). However, there were no strain differences in basal spermidine and spermine levels between the two. After acute stress, the PU levels in frontal cortex and hippocampus (15.99 +/- 0.45 and 14.10 +/- 0.95 nmol/g tissue, respectively) were significantly increased in SHR as compared to the non-stressed SHR (11.03 +/- 0.81 and 11.36 +/- 0.33 nmol/g tissue, respectively). In WKY rats, the PU level was significantly increased by acute stress in frontal cortex (11.68 +/- 1.12 nmol/g tissue) as compared to the non-stressed WKY (6.90 +/- 1.44 nmol/g tissue). After chronic stress, the PU levels in frontal cortex and hippocampus of SHR (12.44 +/- 0.54 and 11.34 +/- 0.66 nmol/g tissue, respectively) significantly decreased as compared to acute-stressed groups (15.99 +/- 0.45 and 14.01 +/- 0.95 nmol/g tissue, respectively). In WKY rats, after chronic stress, the PU level was significantly decreased in frontal cortex (5.73 +/- 0.36 nmol/g tissue) as compared to acute-stressed groups (11.68 +/- 1.12 nmol/g tissue). The PU levels in frontal cortex and hippocampus of acute-stressed (15.99 +/- 0.45 nmol/g tissue and 14.10 +/- 0.95 nmol/g tissue, respectively) and chronic-stressed (12.44 +/- 0.54 and 11.34 +/- 0.66 nmol/g tissue, respectively) SHR were significantly higher than acute-stressed (11.68 +/- 1.12 and 9.76 +/- 0.45 nmol/g tissue, respectively) and chronic-stressed (5.73 +/- 0.36 and 8.44 +/- 0.71 nmol/g tissue, respectively) WKY rats. The present study provides the higher basal PU level and stress-induced PU response in SHR as compared to WKY rats may be related to enhanced response of hypothalamic-pituitary-adrenocortical axis and sympathetic influence that may significantly contribute to the development of hypertension in SHR.
authors.affiliation: Department of Psychiatry, School of Medicine, Keimyung University, 194 Dongsan dong, Taegu, 700-712 South Korea
authors.forename: Hyung Seok
authors.initials: HS
authors.lastname: Sohn
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Park
authors.forename: Seong Ryong
authors.initials: SR
authors.lastname: Lee
mesh_terms: D000208:Acute Disease; D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism*; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000503:physiopathology; D002908:Chronic Disease; D005625:Frontal Lobe / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism / Q000503:physiopathology; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D007030:Hypothalamo-Hypophyseal System / Q000254:growth & development / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012149:Restraint, Physical; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000254:growth & development / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D015317:Biogenic Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine