[Coloration of cytologic thick sections containing biomaterials with silver methenamine. Usefulness for scanning electron microscopy].
Prostheses implanted in hard tissues cannot be processed for electron microscopic examination or microanalysis in the same way as those in other tissues. For these reasons, we have developed a method allowing light and electron microscopic studies as well as microanalysis of the interface between bone and biomaterials after the sections glycoproteins have been stained with silver methenamine. Silver can be evidenced by SEM in back scattered mode.


METHODS
Tranverse sections of a human femur containing an HA-coated prostheses were obtained with a diamond saw and ground to a thickness of 50-100 microns. The sections were stained in a microwave oven using a 1% silver methenamine solution. They have been examined by back-scatter SEM operated at 25 KV. EDS has been performed on cellular inclusions and extracellular bone matrix.


RESULTS
Type I and III collagen fibers, and reticulin fibers were stained. The mineralized matrix was heavily colored. At the cell level, the nuclear and cytoplasm membranes, the chromatin and ribosomes were shown. The characteristic peaks of the Ag spectrum are distinct from those of the elements used in orthopaedic biomaterials and did not impair their identification.
Frayssinet
P
P
College of Medicine of Ohio, Toledo, OH, USA.
Gineste
L
L
Giammara
B
B
Hanker
J S
JS
D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000648:ultrastructure*; D002642:Chick Embryo; D004396:Coloring Agents; D006801:Humans; D008709:Methenamine; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants
D001672:Biocompatible Materials; D004396:Coloring Agents; D008709:Methenamine
Coloration de coupes histologiques épaisses contenant des biomatériaux par la silver methenamine. Utilité pour l'examen en microscopie électronique à balayage.