[Alteration of immunological properties of bacterial lipopolysaccharide by calcium hydroxide treatment].
OBJECTIVE
To study alteration of immunological properties of LPS by Ca(OH)2 treatment, and to establish an experimental model in which ThP-1 cell line was cultured in vitro to observe the alteration of immunological properties of LPS.


METHODS
ThP-1 cell line was used to establish a culture model in vitro. Amount of TNF-alpha released from stimulated cells acted as the index on immunological properties of the stimuli and alteration of immunological properties of LPS by Ca(OH)2 treatment.


RESULTS
Within the range of observed LPS concentrations (10 micrograms/L-10 mg/L), the original immunological properties of LPS decreased significantly after treatment by Ca(OH)2 for a week (P < 0.001). When pH > or = 12.30, the amount of TNF-alpha excreted by monocyte did not differ significantly from the control group (P > 0.05).


CONCLUSIONS
After treatment by Ca(OH)2 for a week, the original immunological properties of LPS decreased significantly. High pH value of Ca(OH)2 played a critical role during the alteration of immunological properties of LPS.
Wang
X
X
Special Dental Department, Peking University School of Stomatology, Beijing 100081, China.
Zhang
C
C
Lin
Q
Q
D002126:Calcium Hydroxide / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D002126:Calcium Hydroxide