title: Filamentous fungi as cell factories for heterologous protein production.
abstract: Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the 'genomics' technology will further develop high-throughput technologies for these organisms.
authors.affiliation: TNO Nutrition and Food Research Institute, Dept of Applied Microbiology and Gene Technology, P.O. Box 360, 3700 AJ, Zeist, The Netherlands. p.punt@voeding.tno.nl
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Punt
authors.forename: Nick
authors.initials: N
authors.lastname: van Biezen
authors.forename: Ana
authors.initials: A
authors.lastname: Conesa
authors.forename: Alwin
authors.initials: A
authors.lastname: Albers
authors.forename: Jeroen
authors.initials: J
authors.lastname: Mangnus
authors.forename: Cees
authors.initials: C
authors.lastname: van den Hondel
mesh_terms: D005285:Fermentation / Q000235:genetics / Q000502:physiology; D005658:Fungi / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D010544:Peroxidases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D015850:Interleukin-6; D011994:Recombinant Proteins; D010544:Peroxidases; C051129:manganese peroxidase