title: Modalities of testing Helicobacter pylori in patients with nonmalignant bile duct diseases.
abstract: OBJECTIVE
  This paper describes the procedure of detection of Helicobacter pylori (H. pylori) in bile specimens in patients suffering from benign diseases of biliary ducts (lithiasis with/without nonspecific cholangitis).
  
  
  METHODS
  The group of 72 patients entering the study consisted of 32 male and 40 female (45% and 55%, respectively). Bile was obtained during ERCP in 68 patients, and during cholecystectomy in 4 patients. A fast urease test (FUT) to determine the existence of H. pylori in gastric mucosa was carried out for all the patients during the endoscopic examination. The existence of genetic material of H. pylori was determined by detection of ureA gene by the method of nested PCR. The results of this reaction were shown by electrophoresis on 10g.L(-1) agarose gel in a band of 256bp.
  
  
  RESULTS
  The majority of the patients included in our study had biliary lithiasis without signs of cholangitis (48 patients, 67%), whereas other patients were complicated by cholangitis (17 patients, 24%). Seven patients (9%) had normal ERCP, forming thus the control group. In the group of patients with lithiasis 26 patients (54.2%) had positive PCR of H. pylori in bile and among the patients with associated cholangitis positive PCR was detected in 9 patients (52.9%). Among the seven patients with normal ERCP only one (14%) had positive PCR of H. pylori. A high percentage of H. pylori infection of gastric mucosa was observed (57 patients, 79%). It was also observed that its slightly higher positivity was in the patients with distinct bile pathology: 81% FUT positive patients in the group with choledocholithiasis alone and 76% in the group with choledocholithiasis associated with cholangitis. Seventy-one percent of the patients with regular findings had positive FUT.
  
  
  CONCLUSIONS
  The prevalence of H. pylori infection both in bile and in gastric mucosa in patients with benign diseases of biliary ducts does not show a statistically significant difference in relation to the prevalence of the same with the patients with normal ERCP. The existence of H. pylori infection possibly does not play a role in pathogenesis of benign biliary diseases.
authors.affiliation: Department of Internal Medicine, Clinical Center Dr Dragisa Misovic-Dedinje, Heroja Milana Tepica 1, 11000 Belgrade, Yugoslavia. mbulajic@EUnet.yu
authors.forename: Milutin
authors.initials: M
authors.lastname: Bulajic
authors.forename: Bojan
authors.initials: B
authors.lastname: Stimec
authors.forename: Miroslav
authors.initials: M
authors.lastname: Milicevic
authors.forename: Matthias
authors.initials: M
authors.lastname: Loehr
authors.forename: Petra
authors.initials: P
authors.lastname: Mueller
authors.forename: Ivan
authors.initials: I
authors.lastname: Boricic
authors.forename: Nada
authors.initials: N
authors.lastname: Kovacevic
authors.forename: Mirko
authors.initials: M
authors.lastname: Bulajic
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001646:Bile / Q000382:microbiology*; D001652:Bile Ducts / Q000473:pathology; D002648:Child; D002760:Cholangiopancreatography, Endoscopic Retrograde; D002761:Cholangitis / Q000150:complications / Q000382:microbiology*; D002769:Cholelithiasis / Q000150:complications / Q000382:microbiology*; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014510:Urease / Q000235:genetics / Q000378:metabolism
chemical_list: D014510:Urease