[mRNA and protein expression of skeletal DHPR(alpha1) and RyRs in diaphragm muscle of rabbits].
To detect mRNA and protein expression of skeletal dihydropridine receptor isoform alpha1 subunit and ryanodine receptor 1 and 3 in diaphragm muscle of rabbits, the coupling mode and characteristics of Ca(2+) release were explored. Reverse transcription PCR, in situ hybridization and immunohistochemical methods were employed. A higher level of mRNA and protein expression of DHPR(alpha1) and RyR(1), and a lower level of mRNA expression of RyR(3) were found. It is suggested that the calcium release unit may consist of skeletal DHPR isoform, RyR(1) and RyR(3), and there may be two kinds of Ca(2+) release mode via conformational changes in linked proteins and Ca(2+)-induced Ca(2+) release (CICR) in diaphragm muscle of rabbits.
Zhang
S Y
SY
Institute of Respiratory Diseases of the New Bridge Hospital, The Third Military Medical University, Chongqing 400037, China.
Liu
G
G
Wang
D L
DL
Guo
X J
XJ
Qian
G S
GS
D000818:Animals; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000096:biosynthesis* / Q000235:genetics; D003964:Diaphragm / Q000378:metabolism*; D005260:Female; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000096:biosynthesis* / Q000235:genetics
D020746:Calcium Channels, L-Type; D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium