title: [Kappa-B nuclear factor and apoptosis of cancerous cells].
abstract: The transcriptional factor NF-kappa B is a key regulator of many biological processes such as the immune response, inflammation, development, cell proliferation and apoptosis. We investigated the role of NF-kappa B in the regulation of the pro-apoptotic protein Bax. We observed increased Bax proteins expression in several cancer cell lines stably expressing a mutated I kappa B-alpha that blocks NF-kappa B activity. Transient transfection experiments showed that NF-kappa B inhibited p53-dependent transactivation of the bax promoter, but this effect was not released by concomitant expression of several cofactors including CBP/p300. We also identified, for the first time, an NF-kappa B binding site in the bax gene promoter but the mutation of this site did not affect NF-kappa B induced inhibition of transcription. The mechanisms responsible for NF-kappa B inhibition of the bax promoter thus remain to be elucidated.
authors.affiliation: Laboratoire de Chimie et Oncologie médicales, C.H.U. du Sart Tilman, U.Lg.
authors.forename: M
authors.initials: M
authors.lastname: Bentires
mesh_terms: D017209:Apoptosis; D016328:NF-kappa B / Q000502:physiology*; D009369:Neoplasms / Q000473:pathology*
chemical_list: D016328:NF-kappa B
vernacular_title: Le facteur nucléaire kappa-B et l'apoptose des cellules cancéreuses.