title: A new, highly sensitive assay for C-reactive protein can aid the differentiation of inflammatory bowel disorders from constipation- and diarrhoea-predominant functional bowel disorders.
abstract: BACKGROUND
  Patients presenting to gastroenterology clinics with symptoms suggestive of lower-bowel disorders often require extensive investigation to differentiate functional from organic disease. C-reactive protein (CRP) is a sensitive marker of systemic inflammation. Levels of CRP are frequently raised in cases of inflammatory bowel disease (IBD). However, using conventional assays, not all cases of IBD have a detectable level.
  
  
  OBJECTIVE
  To determine whether a new highly sensitive CRP enzyme-linked immunosorbent assay (ELISA) can aid the differentiation between IBD and functional bowel disorders (FBDs) in gastroenterology outpatients presenting with lower-bowel symptoms.
  
  
  METHODS
  Serum was taken from 224 subjects attending a gastroenterology outpatient clinic. Of these, 203 were new patients and 21 were follow-up patients with quiescent colitis. The serum was analysed using a sensitive in-house ELISA. All new patients had a rigid sigmoidoscopy and rectal biopsy. Patients were investigated as deemed appropriate by the attending physician. Notes were reviewed after at least 6 months to determine the final diagnosis.
  
  
  RESULTS
  A cut-off value of 2.3 mg/l had a sensitivity of 100% and a specificity of 67% in differentiating FBD from new cases of IBD. The geometric mean CRP was 0.383 mg/l in the constipation-predominant FBD group, 1.435 mg/l in diarrhoea-predominant FBD, 1.455 mg/l in quiescent IBD, 8.892 mg/l in newly presenting cases of ulcerative colitis, and 13.123 mg/l in newly presenting cases of Crohn's disease.
  
  
  CONCLUSIONS
  A new, highly sensitive assay for CRP may help to distinguish FBD from IBD.
authors.affiliation: Mayday University Hospital, London Road, Thornton Heath, Surrey CR7 7YE, UK.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Poullis
authors.forename: Sameer
authors.initials: S
authors.lastname: Zar
authors.forename: Krishna K
authors.initials: KK
authors.lastname: Sundaram
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Moodie
authors.forename: Paul
authors.initials: P
authors.lastname: Risley
authors.forename: Andrew
authors.initials: A
authors.lastname: Theodossi
authors.forename: Michael A
authors.initials: MA
authors.lastname: Mendall
mesh_terms: D000328:Adult; D002097:C-Reactive Protein / Q000032:analysis*; D003109:Colonic Diseases, Functional / Q000175:diagnosis*; D003248:Constipation / Q000209:etiology; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000209:etiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis*; D008297:Male; D011446:Prospective Studies; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D002097:C-Reactive Protein