The heterogeneity of the glycosylation of alpha-1-acid glycoprotein between the sera and synovial fluid in rheumatoid arthritis.
Alpha-1-acid glycoprotein (AGP) is a plasma glycoprotein produced by the liver that undergoes increased production and altered glycosylation in several physiological and pathological conditions including rheumatoid arthritis. To date, although present in the synovial fluid of rheumatoid arthritis patients, there has been no evidence for the separate extra-hepatic production of AGP. This study indicates that there could be a localized production of AGP in rheumatoid synovial fluid by demonstrating that the glycosylation patterns of AGP differed between the serum and synovial fluid in the same rheumatoid patient. Serum AGP was largely composed of fucosylated tri- and tetra-antennary oligosaccharide chains while the synovial fluid contained mainly bi-antennary chains that were fucosylated to a lesser extent. This structural heterogeneity of glycosylation resulted in functional diversity; serum but not synovial AGP is able to inhibit binding to the cell adhesion molecule E-selectin through expression of antigen sialyl Lewis X.
Smith
Kevin D
KD
Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, Scotland, UK. k.d.smith@strath.ac.uk
Pollacchi
Aileen
A
Field
Max
M
Watson
John
J
D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006031:Glycosylation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009961:Orosomucoid / Q000737:chemistry / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism*
D009961:Orosomucoid; D003208:Concanavalin A