title: Occurrence of 20S RNA and 23S RNA replicons in industrial yeast strains and their variation under nutritional stress conditions.
abstract: We have characterized industrial yeast strains used in the brewing, baking, and winemaking industries for the presence or absence of cytoplasmic single-stranded 20S and 23S RNAs. Furthermore, the variation of intracellular concentrations of these replicons in brewing and laboratory strains under nutritional stress conditions was determined. Our results show a correlation between the relative abundance of these replicons and exposure of yeast to nutritionally stressful conditions, indicating that these RNAs could be employed as molecular probes to evaluate the exposure of 20S(+) and/or 23S(+) yeast strains to stress situations during industrial manipulation. During this study, several 20S(-)23S(+) Saccharomyces cerevisiae strains were isolated and identified. This is the first time that a yeast strain containing only 23S RNA has been reported, demonstrating that 20S RNA is not required for 23S RNA replication.
authors.affiliation: Asociación de Investigación de Cerveza y Malta (INVESCEMA),C/ Almagro 24, 8010 Madrid, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: López
authors.forename: Rosario
authors.initials: R
authors.lastname: Gil
authors.forename: José
authors.initials: J
authors.lastname: Vicente Carbonell
authors.forename: Alfonso
authors.initials: A
authors.lastname: Navarro
mesh_terms: D015152:Blotting, Northern; D003470:Culture Media; D003593:Cytoplasm / Q000235:genetics; D007218:Industrial Microbiology; D015335:Molecular Probes; D012331:RNA, Fungal / Q000032:analysis*; D012338:RNA, Ribosomal, 23S / Q000032:analysis*; D012093:Replicon; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
chemical_list: D003470:Culture Media; D015335:Molecular Probes; D012331:RNA, Fungal; D012338:RNA, Ribosomal, 23S