title: Role of antioxidant systems in induced nutritional pancreatic atrophy in chicken.
abstract: One-day-old chicks were reared using diets differing in their vitamin E and/or selenium content. The purpose of this research was to detect any possible imbalance in the antioxidant defense system, which could be related to development of nutritional pancreatic atrophy. Mitochondrial membranes from animals deficient in both nutrients, or just vitamin E, submitted to peroxidizability 'in vitro' had the production of TBARS greatly enhanced. Measurements of the 2-GSH/GSSG ratio suggested that selenium and vitamin E, the latter in higher magnitude, were responsible for maintenance of the reducing capacity of the cell. Enzymatic defense systems against oxidative stress were also studied. The results indicated that the total antioxidant enzymatic activity of pancreatic cells was not sufficient to scavenge all the ROS generated in the nutritionally deficient animals. The present study suggests that nutritional deficiency of selenium and/or vitamin E generates one imbalance between pro-oxidant and antioxidant systems in chicken pancreas, leading to oxidative stress and pancreatic atrophy.
authors.affiliation: Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Av. Duque de Caxias Norte, 225, 13635-900, Pirassununga/SP, Brazil.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Avanzo
authors.forename: Cássio
authors.initials: C
authors.lastname: Xavier de Mendonça Junior
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Cerqueira Cesar
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001284:Atrophy; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism; D010179:Pancreas / Q000473:pathology*; D012643:Selenium / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000494:pharmacology; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002374:Catalase; D005978:Glutathione; D012643:Selenium; D010100:Oxygen