title: A bivalent Huntingtin binding peptide suppresses polyglutamine aggregation and pathogenesis in Drosophila.
abstract: Huntington disease is caused by the expansion of a polyglutamine repeat in the Huntingtin protein (Htt) that leads to degeneration of neurons in the central nervous system and the appearance of visible aggregates within neurons. We have developed and tested suppressor polypeptides that bind mutant Htt and interfere with the process of aggregation in cell culture. In a Drosophila model, the most potent suppressor inhibits both adult lethality and photoreceptor neuron degeneration. The appearance of aggregates in photoreceptor neurons correlates strongly with the occurrence of pathology, and expression of suppressor polypeptides delays and limits the appearance of aggregates and protects photoreceptor neurons. These results suggest that targeting the protein interactions leading to aggregate formation may be beneficial for the design and development of therapeutic agents for Huntington disease.
authors.affiliation: Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: Aleksey
authors.initials: A
authors.lastname: Kazantsev
authors.forename: Heli A
authors.initials: HA
authors.lastname: Walker
authors.forename: Natalia
authors.initials: N
authors.lastname: Slepko
authors.forename: James E
authors.initials: JE
authors.lastname: Bear
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Preisinger
authors.forename: Joan S
authors.initials: JS
authors.lastname: Steffan
authors.forename: Ya-Zhen
authors.initials: YZ
authors.lastname: Zhu
authors.forename: Frank B
authors.initials: FB
authors.lastname: Gertler
authors.forename: David E
authors.initials: DE
authors.lastname: Housman
authors.forename: J Lawrence
authors.initials: JL
authors.lastname: Marsh
authors.forename: Leslie M
authors.initials: LM
authors.lastname: Thompson
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019556:COS Cells; D002460:Cell Line; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D000939:Epitopes; D005982:Glutathione Transferase / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008969:Molecular Sequence Data; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics*; D010455:Peptides / Q000737:chemistry*; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D013489:Suppression, Genetic; D013997:Time Factors; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D000939:Epitopes; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; C097188:polyglutamine; D005982:Glutathione Transferase