Increase in the sensitivity of dengue diagnosis by combination of reverse transcriptase-polymerase chain reaction and passage on cell cultures.
We passaged 52 serum samples from dengue patients on C6/36 cells for 7 days and checked the culture fluids by RT-PCR. Two serum samples, which were negative by direct RT-PCR, became positive. One sample was collected on fever day 1 and the other on fever day 2. Results indicate that combination of reverse transcriptase-polymerase chain reaction (RT-PCR) with passage of serum samples on C6/36 cells increases the sensitivity of dengue diagnosis.
Yamada
K
K
Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan. kyamada@nih.go.jp
Takasaki
T
T
Nawa
M
M
Kurane
I
I
D002460:Cell Line; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012692:Serial Passage