title: The effect on MHC class II expression and apoptosis in placenta by IFNgamma administration.
abstract: To investigate the possible mechanisms by which interferon gamma (IFNgamma) affects pregnancy, the expression of class II major histocompatibility complex (MHC) antigens and cleavage of DNA that is a hallmark of apoptosis in the placenta were examined by molecular biochemical techniques, and progesterone levels were examined by radioimmunoassay. The semi-quantitative analysis with reverse transcription-polymerase chain reaction showed that the expression of MHC class II antigen in placenta increased when rabbits were treated with high doses of IFNgamma compared with the control. However, immunohistochemical study suggested that IFNgamma did not affect MHC class II expression in trophoblasts, but had a stimulatory effect on its expression in maternal decidua and placental lymphocytes. DNA fragmentation analysis and terminal deoxynucleotidyl mediated-deoxyuridine triphosphate nick end labeling (TUNEL) assay indicated that the cleavage of DNA was detected in the placenta in both normal and IFNgamma-treated pregnancy. Quantitative analysis of apoptotic cells revealed an increase in trophoblasts treated with IFNgamma compared to those in normal pregnancy. Moreover, progesterone, which plays an important role in pregnancy, was reduced significantly in rabbits treated with IFNgamma. The results suggested that IFNgamma exerted its deleterious effect on pregnancy by inducing apoptosis in trophoblasts and by reducing the production of progesterone.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy Sciences, Beijing 100080, People's Republic of China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Jing-pian
authors.initials: JP
authors.lastname: Peng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D053938:DNA Fragmentation; D003656:Decidua / Q000276:immunology; D005260:Female; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000235:genetics*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D007371:Interferon-gamma / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D010920:Placenta / Q000166:cytology* / Q000276:immunology*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012313:RNA / Q000032:analysis; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000276:immunology
chemical_list: D000949:Histocompatibility Antigens Class II; D011374:Progesterone; D012313:RNA; D007371:Interferon-gamma