[Effect of hydrogen peroxide on expression of interleukin-2 gene in mouse splenocytes].
The effect of hydrogen peroxide on interleukin-2(IL-2) production and IL-2 mRNA expression in mouse splenocytes was detected by cellular immunological and molecular biological methods. The results were that the level of IL-2 activity was significantly elevated in 10(-4) mol.L-1 of H2O2 as compared with the control group (P < 0.01), was obviously inhibited in 10(-3)-10(-2) mol.L-1 of H2O2(P < 0.01), and had no effect in 10(-8)-10(-5) mol.L-1 of H2O2. The results of dot blotting were that 10(-4) mol.L-1 of H2O2 enhanced IL-2 mRNA expression, while 10(-3) mol.L-1 inhibited it, and 10(-5) mol.L-1 had no effect in mouse splenocytes. These results suggest that the effect of H2O2 on IL-2 production may be mediated by regulating IL-2 mRNA expression in mouse splenocytes.
Chen
J
J
Research Laboratory of Immunology, Hunan Medical University, Changsha 410078.
Guo
S
S
D000818:Animals; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
D007376:Interleukin-2; D012333:RNA, Messenger; D006861:Hydrogen Peroxide