[Effects of regulatory peptides on adhesion of eosinophil to bronchial epithelial cells].
To explore the roles of regulatory peptides in the process of various anaphylactic inflammation of the airway, we observed the influence of four peptides, i.e., vasoactive intestinal peptide (VIP), epidermal growth factor (EGF), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP), on the adhesion of eosinophil (EOS) to unstimulated and O(3)-stressed bronchial epithelial cells (BEC). From the experiments we observed that VIP and EGF decreased EOS adherence to O(3)-stressed BEC and downregulated airway inflammation; ET-1 and CGRP increased the adhesion of EOS to BEC in the inflammatory process; and CGRP aggravated O(3)-stressed reactions. The effects of ET-1 and CGRP were inhibited by W(7)and H(7). Anti-ICAM-1 antibody inhibited the adhesion of EOS to BEC, which brings to light that EOS adherence to BEC may be related to the expression of ICAM-1 of BEC.
Tan
Yong
Y
Department of Physiology, Xiang Ya Medical College, Central South University, Changsha 410078, Qinxq@public.cs.hn.cn
Qin
Xiao-Qun
XQ
Guan
Cha-Xiang
CX
Zhang
Chang-Qing
CQ
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000494:pharmacology; D004804:Eosinophils / Q000502:physiology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000502:physiology*; D008297:Male; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D000906:Antibodies; D019332:Endothelin-1; D018799:Intercellular Adhesion Molecule-1; D014660:Vasoactive Intestinal Peptide; D004815:Epidermal Growth Factor