title: Influence of long-term treatment with tetracycline and niacinamide on antibody production in dogs with discoid lupus erythematosus.
abstract: OBJECTIVE
  To evaluate the effect of long-term treatment with tetracycline and niacinamide on antibody production in dogs by measuring postvaccinal serum concentrations of antibodies against canine parvovirus and canine distemper virus.
  
  
  METHODS
  10 dogs receiving long-term treatment with tetracycline and niacinamide (treatment group) and 10 healthy dogs (control group).
  
  
  METHODS
  The treatment group included 9 dogs with discoid lupus erythematosus and 1 dog with pemphigus foliaceus on long-term treatment (> 12 months) with tetracycline and niacinamide. The control group included 10 healthy dogs with no clinical signs of disease and no administered medications for the past 3 months. Blood samples were obtained from all dogs by jugular venipuncture. Serum antibody titers against canine parvovirus and canine distemper virus antigens were measured, using hemaglutination inhibition and serum neutralization, respectively, and compared between groups.
  
  
  RESULTS
  A significant difference in antibody titers between treatment- and control-group dogs was not found. All dogs had protective antibody titers against canine distemper virus, and 8 of 10 dogs from each group had protective titers against canine parvovirus infection.
  
  
  CONCLUSIONS
  These results provide evidence that long-term treatment with tetracycline and niacinamide does not interfere with routine vaccinations and thus does not seem to influence antibody production in dogs.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
authors.forename: Ralf S
authors.initials: RS
authors.lastname: Mueller
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Fieseler
authors.forename: Sonya V
authors.initials: SV
authors.lastname: Bettenay
authors.forename: Rodney A W
authors.initials: RA
authors.lastname: Rosychuk
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D004217:Distemper Virus, Canine / Q000276:immunology / Q000302:isolation & purification; D004283:Dog Diseases / Q000188:drug therapy* / Q000276:immunology; D004285:Dogs; D005260:Female; D008179:Lupus Erythematosus, Discoid / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D009536:Niacinamide / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D010322:Parvoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D017992:Parvovirus / Q000276:immunology / Q000302:isolation & purification; D013754:Tetracyclines; D014611:Vaccination / Q000662:veterinary
chemical_list: D000900:Anti-Bacterial Agents; D000914:Antibodies, Viral; D013754:Tetracyclines; D009536:Niacinamide