[Research on the mechanism of the fatty acid composition on the tumorigenic danger induced by chemical tumorigenic material].
Rats were given 5 kinds of diets which contained 15% (w/w) fat and different fatty acids composition. The rats were given methyl-nitrosurea (MNU) to induce colonic tumor. Proliferation cell nuclear antigen (PCNA), cell kinetics, membrane fluidity, the activity of alkaline phosphatase (ALP) and the content of prostaglandin E2(PGE2) in colonic mucosa were determined in order to assess the fatty acids composition on the colonic cell tumorigenesis. The results indicated that the cells of PCNA, cells of PI labeled in S period and the activity of ALP were the highest in the 3rd group which contained lowest saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) and highest n-6 polyunsaturated fatty acids. Whereas, these indexes and PGE2 were lowest and membrane fluidity was the best in the 4th group which contained the highest 1-3 PUFA. It is considered that the inhibition tumorigenesis of n-3 PUFA may be related to its effects of decreaing PCNA, PGE2, cells in S period and increasing membrane fluidity.
Zhou
S
S
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin 150001, China.
Chen
B
B
Wang
P
P
Wang
G
G
D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced*; D015232:Dinoprostone / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry* / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity; D008770:Methylnitrosourea; D051381:Rats; D017207:Rats, Sprague-Dawley
D005227:Fatty Acids; D008770:Methylnitrosourea; D015232:Dinoprostone