[The expression of glucocorticoid receptor beta messenger RNA in peripheral white blood cells of hormone-resistant nephrotic syndrome patients].
OBJECTIVE
To study the molecular mechanism contributing to hormone resistance of nephrotic syndrome.


METHODS
The specific hormone binding sites of receptor was determined by radioligand binding assay. Messenger RNA of receptor was determined by quantitative reverse transcription-polymerase chain reaction.


RESULTS
No significant differences of specific hormone binding sites of peripheral white blood cells were observed among hormone-sensitive patients, hormone-resistant patients and healthy volunteers. However, in hormone-resistant patients, GR alpha/GR beta mRNA ratio decreased significantly as compared with that of hormone-sensitive patients and healthy volunteers.


CONCLUSIONS
Results of the experiments indicated that the increased expression of GR beta, which leads to the decrease of the GR alpha/GR beta ratio, may be one of the mechanism responsible for hormone resistance in some nephrotic syndrome patients.
Liu
Y
Y
Department of Pathophysiology, Second Military Medical University, Shanghai 200433, China.
Song
L
L
Li
B
B
D001665:Binding Sites / Q000502:physiology; D004351:Drug Resistance / Q000502:physiology; D015870:Gene Expression; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D009404:Nephrotic Syndrome / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta