Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries.
Blueberries and blackberries grown at various locations in Georgia were collected and analyzed for flavonoids, total anthocyanins, total polyphenols, and Trolox-equivalent antioxidant capacity (TEAC). Each sample was analyzed for phenolic acids (gallic acid, p-hydroxybenzoic acid, caffeic acid, ferulic acid, and ellagic acid) and flavonoids (catechin, epicatechin, myricetin, quercetin, and kaempferol). A high-performance liquid chromatographic (HPLC) method with photodiode array detection was used for analysis. Compounds were analyzed as aglycons after acid hydrolysis with 1.2 M HCl. Identification of each compound was based on retention time and UV spectra by comparison with pure commercial standards. Phenolic acids ranged from 0.19 to 258.90 mg/100 g fresh weight (FW), and flavonoids ranged from 2.50 to 387.48 mg/100 g FW. Total polyphenols ranged from 261.95 to 929.62 mg/100 g FW, and total anthocyanins ranged from 12.70 to 197.34 mg/100 g FW. TEAC values varied from 8.11 to a maximum of 38.29 microM/g FW. A linear relationship was observed between TEAC values and total polyphenols or total anthocyanins. The data indicate that blueberries and blackberries are rich sources of antioxidants.
Sellappan
Subramani
S
Department of Food Science and Technology, The University of Georgia, Athens, Georgia 30602-7610, USA.
Akoh
Casimir C
CC
Krewer
Gerard
G
D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D029798:Blueberry Plants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D005845:Georgia; D010636:Phenols / Q000032:analysis*; D011108:Polymers / Q000032:analysis; D027824:Rosaceae / Q000737:chemistry*
D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D011108:Polymers