A technical note on microsatellite DNA instability studied in archival paraffin-embedded tissues.
The authors present brief overview of problems associated with analysis of microsatellite sequences in DNA from archival paraffin-embedded tissue. This methodology can serve several diagnostic and research purposes provided appropriate precautions are taken to extract DNA of acceptable quality with small admixture of contaminants. Also PCR amplification step requires some modifications compared to routine reactions involving DNA from fresh or freshly-frozen tissues.
Rudzki
Z
Z
Department of Pathomorphology, Jagiellonian University Medical College, Kraków.
Zazula
M
M
Stachura
J
J
D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods
D004247:DNA