title: Activation of mislocalized opsin kills rod cells: a novel mechanism for rod cell death in retinal disease.
abstract: Rod photoreceptors are highly compartmentalized sensory neurons that maintain strict ultrastructural and molecular polarity. Structural subdivisions include the outer segment, inner segment, cell body, and synaptic terminal. The visual pigment rhodopsin is found predominantly in membranes of the rod cell outer segment but becomes mislocalized, appearing throughout the plasma membrane of the cell in many retinal diseases and injuries. Currently, there is no known link between rhodopsin redistribution and rod cell death. We propose that activation of mislocalized rhodopsin kills rod cells by stimulating normally inaccessible signaling pathways. This hypothesis was tested in primary retinal cell cultures, which contain photoreceptors. In rod photoreceptors, opsin immunofluorescence occurred throughout the rod cell plasma membrane. Activation of this mislocalized opsin by photostimulation after formation of isorhodopsin or by incubation with beta-ionone (opsin agonist) killed 19-30% of rod cells. Rod cell death was apoptotic, as indicated by marked chromatin condensation and the requirement for caspase-3 activation. Rod cell death could be induced by forskolin (adenylate cyclase agonist), and conversely, beta-ionone-induced cell death could be blocked by cotreatment with SQ22536 (an adenylate cyclase inhibitor). Pertussis toxin (a G protein inhibitor) also blocked beta-ionone-induced cell death. The data support a mechanism by which activation of mislocalized opsin initiates apoptotic rod cell death through G protein stimulation of adenylate cyclase.
authors.affiliation: Neurosciences Department, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, 185 South Orange Ave, Newark, NJ 07103, USA. alfinipe@umdnj.edu
authors.forename: Peter D
authors.initials: PD
authors.lastname: Alfinito
authors.forename: Ellen
authors.initials: E
authors.lastname: Townes-Anderson
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D037361:Adenylate Cyclase Toxin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D005576:Colforsin / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D037342:Pertussis Toxin; D012164:Retinal Diseases / Q000378:metabolism*; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects* / Q000473:pathology*; D017299:Rod Opsins / Q000494:pharmacology*; D015398:Signal Transduction; D013997:Time Factors; D014562:Caudata; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D037361:Adenylate Cyclase Toxin; D002843:Chromatin; D004791:Enzyme Inhibitors; D017299:Rod Opsins; D010566:Virulence Factors, Bordetella; C017759:9-(tetrahydro-2-furyl)-adenine; D005576:Colforsin; D037342:Pertussis Toxin; D053148:Caspase 3; D020169:Caspases; D000262:Adenylyl Cyclases; D000225:Adenine