title: Cloning and molecular characterization of a SDS-activated tyrosinase from Marinomonas mediterranea.
abstract: The sequence of the tyrosinase gene cloned from Marinomonas mediterranea is reported. It is the second tyrosinase cloned from a Gram negative bacterium. Its size is higher than that of Gram positive tyrosinases from Streptomyces, and more similar to the eukaryotic enzymes. Its sequence shares the features of copper-binding sites found in all tyrosinases. Based in the comparison of tyrosinases from all types of organisms, an extension of the characteristic signatures existing at Prosite is proposed. This tyrosinase shares with some plant and amphibian tyrosinases a strong specific activation by submicellar concentrations of SDS. Intrinsic fluorescence and kinetic properties indicate that the activation is caused by an SDS-dependent conformational change that facilitates the substrate accessibility to the dicopper active site.
authors.affiliation: Department of Genetics and Microbiology, University of Murcia, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: López-Serrano
authors.forename: Antonio
authors.initials: A
authors.lastname: Sanchez-Amat
authors.forename: Francisco
authors.initials: F
authors.lastname: Solano
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003300:Copper / Q000378:metabolism; D004789:Enzyme Activation; D005453:Fluorescence; D020563:Gammaproteobacteria / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011487:Protein Conformation; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D012967:Sodium Dodecyl Sulfate; D003300:Copper; D014442:Monophenol Monooxygenase