Gastrin, somatostatin, G and D cells of gastric ulcer in rats.
OBJECTIVE
To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats.


METHODS
Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system.


RESULTS
In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process.


CONCLUSIONS
In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
Sun
Feng-Peng
FP
Department of Gastroenterology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China. sci@china.com
Song
Yu-Gang
YG
Cheng
Wei
W
Zhao
Tong
T
Yao
Yong-Li
YL
D000818:Animals; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D019863:Gastrin-Secreting Cells / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000378:metabolism*; D019864:Somatostatin-Secreting Cells / Q000378:metabolism*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000503:physiopathology*
D005755:Gastrins; D013004:Somatostatin