[Study on the loss of heterozygosity and expression of transglutaminase 3 gene in laryngeal carcinoma].
OBJECTIVE
To investigate the role of transglutaminase 3 (TGM3) gene in laryngeal carcinogenesis.


METHODS
The authors detected the deletion indirectly through loss of heterozygosity (LOH) analysis at DNA level using 4 STR primers within and near TGM3 gene in 72 cases, and detected the differential expression of TGM3 gene in 8 cases of paired normal and cancerous tissue of laryngeal carcinoma by Northern blot.


RESULTS
LOH was found existing in all of the microsatellite loci, and the LOH frequencies were 25.76%, 20.00%, 38.10% and 18.75% at D20S17, D20S607, D20S99 and D20S841 respectively; LOH concerning at least one polymorphism locus accounted for 61.11%. No correlation of clinical stage, lymph node metastasis and differentiation with the LOH of TGM3 gene was observed, P>0.05. TGM3 gene expressed significantly higher in normal tissues than in paired cancerous tissues.


CONCLUSIONS
TGM3 gene might play an important role in laryngeal carcinogenesis  and further researches will be needed to clarify the possible mechanisms.
He
Guang
G
Department of Medical Genetics, China Medical University, Shenyang, Liaoning 110001 P. R. China. klsun@mail.cmu.edu.cn
Zhao
Zhen
Z
Fu
Weineng
W
Sun
Xinghe
X
Xu
Zhenming
Z
Sun
Kailai
K
D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000201:enzymology / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011503:Transglutaminases / Q000235:genetics*
D002135:Calcium-Binding Proteins; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; C498712:TGM3 protein, human; D011503:Transglutaminases