A PCR-RFLP typing method for adhesion molecule gene polymorphisms and allele frequencies in a normal UK population.
We report simple and reproducible PCR-RFLP typing methods for the polymorphisms in the ICAM-1, E-selectin and PECAM-1 genes. The genotype and allele frequencies detected in a normal UK population did not deviate significantly from the Hardy-Weinberg equilibrium; neither did they differ from frequencies previously reported using SSP or SSCP methods.
Gbadegesin
R A
RA
Department of Nephrology, Royal Manchester Children's Hospital, Pendlebury, Manchester M27 4HA, UK.
Watson
C J
CJ
Cotton
S A
SA
Brenchley
P E C
PE
Webb
N J A
NJ
D000483:Alleles; D019040:E-Selectin / Q000235:genetics*; D005787:Gene Frequency; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D006113:United Kingdom
D019040:E-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1