DNA damage and apoptosis in the mussel Mytilus galloprovincialis.
The effects of known genotoxic substances (4-nitroquinoline-N-oxide, benzo[a]pyrene, teniposide, etoposide, cycloheximide, tributyltin) on human cells (FLC, HL-60) and on mussels were investigated. The correlations between formation of DNA strand breaks and DNA fragmentation characteristic for the process of apoptosis were estimated. Strand breaks induced by 4-nitroquinoline-N-oxide and benzo[a]pyrene did not correlate with DNA fragmentation detected in the process of apoptosis. Induction of internucleosomal DNA fragmentation in HL-60 cells was initiated by teniposide, etoposide and tributyltin, while in the gills of mussels this was detected only with tributyltin. Levels of DNA strand breaks in natural mussel populations, living at locations under the influence of urban and industrial wastes, do not mirror the apoptotic processes.
Micić
Milena
M
Center for Marine Research, Rudjer Bosković Institute, Rovinj, Croatia. micic@cim.irb.hr
Bihari
Nevenka
N
Jaksić
Zeljko
Z
Müller
Werner E G
WE
Batel
Renato
R
D000818:Animals; D017209:Apoptosis; D049872:Bivalvia / Q000235:genetics*; D018929:Cell Culture Techniques; D004249:DNA Damage; D005880:Gills; D006801:Humans; D007220:Industrial Waste; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007220:Industrial Waste; D014874:Water Pollutants, Chemical