title: Identification of a novel protein p59(scr), which is expressed at specific stages of mouse spermatogenesis.
abstract: A novel cDNA-encoding polypeptide of 545 amino acid residues was identified from a mouse testis cDNA library. The transcript of this gene, p59(scr), was predominantly expressed in the testis and was developmentally regulated during spermatogenesis. The encoded protein was expressed in spermatocytes and round spermatids within seminiferous tubules of the adult testis. Using an adult-mouse model of experimental unilateral cryptorchidism, it was observed that the expression of the p59(scr) mRNA was reduced in the cryptorchid testes in association with destruction of spermatogenesis. In vitro heat stress experiment further demonstrated that p59(scr) mRNA was more sensitive to heat stress than the other mRNA species, such as germ-cell-specific meiosis-activating kinase (mak) gene and a housekeeping beta-actin gene. Our results suggest that this novel p59(scr) gene is involved in spermatogenesis and may play an important role in development of testicular germ cells.
authors.affiliation: Department of Immunology, Tokai University School of Medicine, Bouseidai, Isehara, Kanagawa, 259-1193, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Senoo
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Hoshino
authors.forename: Naoko
authors.initials: N
authors.lastname: Mochida
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Matsumura
authors.forename: Sonoko
authors.initials: S
authors.lastname: Habu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D003456:Cryptorchidism / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D006358:Hot Temperature; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000473:pathology
chemical_list: D011506:Proteins; D012333:RNA, Messenger; C486737:SPATS2 protein, human; C454107:Spats2 protein, mouse; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081931:MAK protein, human; C509962:Mak protein, mouse