Myostatin is an inhibitor of myogenic differentiation.
Myostatin (MSTN), a transforming growth factor (TGF)-beta superfamily member, has been shown to negatively regulate muscle growth by inhibiting muscle precursor cell proliferation. Here, we stably transfected C(2)C(12) cells with mouse MSTN cDNA to investigate its possible role in myoblast differentiation. We found that MSTN cDNA overexpression reversibly inhibits the myogenic process by downregulating mRNA levels of the muscle regulatory factors myoD and myogenin, as well as the activity of their downstream target creatine kinase. Taking into consideration that MSTN expression during development is restricted to muscle, our results suggest that MSTN probably regulates myogenic differentiation by an autocrine mechanism.
Ríos
Ramón
R
Departamento de Fisiología, Facultad de Medicina, Universidad de Santiago de Compostela, San Francisco s/n, 15705 Santiago de Compostela, Spain.
Carneiro
Isabel
I
Arce
Víctor M
VM
Devesa
Jesús
J
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D003402:Creatine Kinase / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D055435:Myostatin; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
C525912:Mstn protein, mouse; D017570:MyoD Protein; D055435:Myostatin; D016212:Transforming Growth Factor beta; D007041:Hypoxanthine Phosphoribosyltransferase; D003402:Creatine Kinase