DNA polymorphism in north indian isolates of Entamoeba histolytica detected by PCR fingerprinting.
Entamoeba histolytica infection in humans is still one of the leading causes of parasitic diseases among the developing countries, including India. It therefore becomes extremely important to characterize this parasite with the aim of preventing and controlling the amoebiasis which it causes. The present study describes for the first time the ability of random amplified polymorphic DNA (RAPD)-PCR fingerprinting strategies to subtype amoebae and to detect considerable genetic variability present among the various North Indian isolates of E. histolytica studied. The number of rapdemes generated by the application of arbitrary primers in RAPD-PCR showed a significant variation in these isolates, both in the size and number of bands, thus revealing the presence of considerable genetic polymorphism in them. Hence, this rapid and easy method could suitably be employed in carrying out significant molecular epidemiological studies and also in the large-scale epidemiological typing of this parasite.
Prakash
Alka
A
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Chakraborti
Anuradha
A
Mahajan
Ramesh Chander
RC
Ganguly
Airmal Kumar
AK
D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis*; D004748:Entamoeba histolytica / Q000235:genetics* / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000453:epidemiology / Q000469:parasitology*; D014644:Genetic Variation; D007194:India / Q000453:epidemiology; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D016054:DNA, Protozoan