Genetic variability within Trypanosoma brucei gambiense: evidence for the circulation of different genotypes in human African trypanosomiasis patients in Côte d'Ivoire.
For 23 Ivoirian patients infected by Trypanosoma-brucei gambiense, isolation and genetic characterization using PCR and microsatellite primers were performed (in 1996-99) using 2 different isolates (A and B) from each patient. When using TBDAC 1/2, 7 genotypes were observed, and DNAs A and B for 2 patients were different. This might be the first evidence of the presence of 2 different genotypes of T. b. gambiense group 1 in the same patient.
Truc
Philippe
P
Institut de Recherche pour le Développement, Département Sociétés et Santé, Laboratoire de Recherche et de Coordination sur les Trypanosomoses, UR 035 Trypanosomoses Africaines, Campus International de Baillarguet, 34398 Montpellier, France. truc@iccnet.cm
Ravel
Sophie
S
Jamonneau
Vincent
V
N'Guessan
Paul
P
Cuny
Gérard
G
D000818:Animals; D007560:Cote d'Ivoire / Q000453:epidemiology; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D014347:Trypanosoma brucei gambiense / Q000235:genetics*; D014353:Trypanosomiasis, African / Q000453:epidemiology / Q000235:genetics*
D007527:Isoenzymes