Effects of reactive oxygen species on lymphokine-activated killer cells in patients with bladder cancer.
OBJECTIVE
To investigate the effect of reactive oxygen species on the proliferation of lymphokine-activated killer (LAK) cells in patients with bladder cancer and their cytolysis to bladder tumor cells.


METHODS
Sodium nitroprusside (SNP) was used as nitric oxide (NO) donor. The superoxide anion (O2-.) was generated in the complete medium (CM) supplemented with N-methylphenazonium methyl sulfate (PMS) 3-120 micromol/L and nicotinamide adenine dinucleotide (NADH) 18 - 600 micromol/L. The hydroxyl radical (.OH) was produced by adding ascorbic acid (AA) 0.5 - 400 micromol/L and ferrous sulfate (Fe2+) 0.05 - 40 micromol/L in CM. LAK cell proliferation and cytotoxicity were assayed in the presence of NO, .OH, or O2-. Bladder cancer cell lines BIU-87 and EJ were cultured as target cells and cytotoxicity of LAK cells were determined by MTT assay.


RESULTS
The proliferation of LAK cells induced by interleukin-2 (IL-2) was inhibited by hydroxyl radical from 48 h to 96 h in a dose-dependent fashion and was inhibited to 34.5 % compared with control at 96 h in the concentration of ascorbic acid 400 micromol/L and ferrous sulfate 40 micromol/L. The inhibition induced by.OH can be overcome by certain concentrations of mannitol or editic acid. On the contrary, the proliferation of LAK cells induced by IL-2 was stimulated by certain concentrations of NO or O2-. The stimulation induced by O2-. can be overcome to control level by superoxide dismutase (SOD) 3 10(5) U/L. Exogenous O2-. resulted in an increase in cytotoxicity of LAK cells against BIU87 and EJ cells. However, the LAK cells cytotoxicity treated with hydroxyl radical or SOD showed no difference as compared with the control.


CONCLUSIONS
NO and O2-. enhanced the proliferation and activation and O2-. up-regulated antitumor cytotoxicity of LAK cells in patients with bladder cancer. The growth of LAK cells induced by IL-2 was down-regulated by hydroxyl radical. The effects of these reactive oxygen species on the proliferation of LAK cells induced by IL-2 were different.
Wang
Zhi-Ping
ZP
Institute of Urology, 2nd Affiliated Hospital of Lanzhou Medical College; Department of Biology, Lanzhou University, Lanzhou 730000, China.
Chen
Yi-Rong
YR
Zheng
Rong-Liang
RL
Lu
Jian-Zhong
JZ
Wang
Jia-Ji
JJ
D002295:Carcinoma, Transitional Cell / Q000276:immunology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000276:immunology* / Q000473:pathology
D007376:Interleukin-2; D017382:Reactive Oxygen Species