Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line.
GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.
Gil-Puig
C
C
Department of Physiology, School of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Blanco
M
M
García-Caballero
T
T
Segura
C
C
Pérez-Fernández
R
R
D000230:Adenocarcinoma / Q000737:chemistry*; D015153:Blotting, Western / Q000379:methods; D001943:Breast Neoplasms / Q000737:chemistry*; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D005260:Female; D013006:Growth Hormone / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger; D013006:Growth Hormone