Improving selenium extraction by sequential enzymatic processes for Se-speciation of selenium-enriched Agaricus bisporus.
Sample preparation methods based on the use of proteolytic and cell wall digesting enzymes for the speciation analysis of selenized mushroom were investigated. The sample (Agaricus bisporus; 160 microg total Se per g sample) was grown on compost supplemented with selenized yeast. Experiments were carried out to elucidate the possible role of the cell wall digesting enzymes--Lysing enzyme and Driselase--in the improvement of extraction efficiency with and without inhibiting proteolysis during cell wall digestion. A 3-step procedure applying Lysing enzyme and pronase gave the highest extraction efficiency (89%); however, the best species recovery was achieved by a one-step proteolytic procedure. All the procedures of selenium speciation were controlled by independent ICP-AES analysis measuring the total amount of selenium.
Dernovics
M
M
Department of Applied Chemistry, Faculty of Food Science, Szent István University, Budapest, Viilányt út, Hungary. dernovics@omega.kee.hu
Stefánka
Zs
Z
Fodor
P
P
D000364:Agaricus / Q000737:chemistry*; D002473:Cell Wall / Q000201:enzymology; D010450:Endopeptidases / Q000737:chemistry; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000737:chemistry*; D018036:Selenium Compounds / Q000032:analysis / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
D005656:Fungal Proteins; D018036:Selenium Compounds; D006026:Glycoside Hydrolases; C035212:driselase; D010450:Endopeptidases