title: The mei3 region of the Schizosaccharomyces pombe genome.
abstract: Expression of the mei3 gene is sufficient to induce meiosis in the fission yeast Schizosaccharomyces pombe. The mei3 gene is located 0.64 Mb from the telomere of the left arm of Sz. pombe chromosome II. We have sequenced and analysed 107 kb of DNA from the mei3 genomic region. The sequence includes 14 known genes (bag1-B, csh3, dps1, gpt1, mei3, mfm3, pac1, prp31, rpl38-1, rpn3, rti1, spa1, spm1 and ubc4) and 26 other open reading frames (ORFs) longer than 100 codons: a density of one protein-coding gene per 2.7 kb. Twenty-one of the 40 ORFs (53%) have introns. In addition there is one lone Tf1 transposon long terminal repeat (LTR), tRNA(Trp) and tRNA(Ser) genes and a 5S rRNA gene. 14 of the novel ORFs show sequence similarities which suggest functions of their products, including a coatomer alpha-subunit, a catechol O-methyltransferase, protein kinase, asparagine synthetase, zinc metalloprotease, acetyltransferase, phosphatidylinositol 4-kinase, inositol polyphosphate phosphatase, GTPase-activating protein, permease, pre-mRNA splicing factor, 20S proteasome component and a thioredoxin-like protein. One predicted protein has similarity to the human Cockayne syndrome protein CSA and one with human GTPase XPA binding protein XAB1. Three ORFs are likely to code for proteins because they have sequence similarity with hypothetical proteins, three encode predicted coiled-coil proteins and four are sequence orphans.
authors.affiliation: School of Biological Sciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter EX4 4QG, UK. S.J.Aves@exeter.ac.uk
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Aves
authors.forename: Cherryl
authors.initials: C
authors.lastname: Hunt
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
authors.forename: Michael H
authors.initials: MH
authors.lastname: Lyne
authors.forename: Valerie
authors.initials: V
authors.lastname: Wood
authors.forename: Marie-Adèle
authors.initials: MA
authors.lastname: Rajandream
authors.forename: Jason
authors.initials: J
authors.lastname: Skelton
authors.forename: Carol M
authors.initials: CM
authors.lastname: Churcher
authors.forename: Timothy
authors.initials: T
authors.lastname: Warren
authors.forename: David
authors.initials: D
authors.lastname: Harris
authors.forename: Rhian
authors.initials: R
authors.lastname: Gwilliam
authors.forename: Barclay G
authors.initials: BG
authors.lastname: Barrell
mesh_terms: D003360:Cosmids; D005656:Fungal Proteins / Q000235:genetics*; D016681:Genome, Fungal; D008540:Meiosis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017421:Sequence Analysis
chemical_list: D005656:Fungal Proteins; C116305:MEI3 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins