Induction of differentiation in rat C6 glioma cells with Saikosaponins.
The effects of saikosaponins (a, b(1), b(2), c, d), isolated from Bupleurum Radix, on the induction of differentiation in rat C6 glioma cells were studied. Saikosaponins a and d were shown to inhibit cell proliferation and alter cell morphology. In addition to cytostasis, the enzymatic activities of glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) were also noticeably increased after treatment with saikosaponin a. Nevertheless, saikosaponin d only showed an increase of GS activity, no significant changes in CNP activity were found. These results suggest that saikosaponin a can induce the differentiation of C6 glioma cells into astrocytes and/or oligodendrocytes, but saikosaponin d can only induce the differentiation of C6 glioma cells into astrocytes.
Tsai
Yan-Jyu
YJ
Department of Pharmacology, Institute of Medical Research, Taipei Medical College, Taipei, Taiwan, R.O.C.
Chen
I-Ling
IL
Horng
Lin-Yea
LY
Wu
Rong-Tsun
RT
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D029972:Bupleurum; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D005974:Glutamate-Ammonia Ligase / Q000187:drug effects / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010727:Phosphoric Diester Hydrolases / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D012502:Sapogenins / Q000494:pharmacology*; D012503:Saponins; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; D010727:Phosphoric Diester Hydrolases; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; C571339:Cnp protein, rat; D005974:Glutamate-Ammonia Ligase; C025759:saikosaponin D