Purification and characterization of 3,3-dihydroxyazetidine from culture medium of Bacillus mesentericus and B. subtilis.
A method is described for the purification of 3,3-dihydroxyazetidine (DHA), which accelerates the growth of Bifidobacterium spp., from the culture medium of Bacillus mesentericus (BM). Purification involved adsorption to an ion-exchange resin; it required less time and gave a higher yield than a previously reported method. Monitoring the inhibition of E. coli NIHJ JC-2, we searched for other strains that produced 3,3-dihydroxyazetidine. We found that not only B. mesentericus TO-A but also B. subtilis IFO13719 produced the compound of interest.
Ozone
Fumiko
F
Department of Microbiology, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan.
Inoue
Yoshihiro
Y
Shiraishi
Akiko
A
Hamashima
Hajime
H
Masuda
Kazuo
K
Shiojima
Kenji
K
Sasatsu
Masanori
M
D001384:Azetidines / Q000302:isolation & purification*; D001407:Bacillus / Q000737:chemistry*; D001412:Bacillus subtilis / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003470:Culture Media / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D019936:Probiotics / Q000494:pharmacology
D001384:Azetidines; D003470:Culture Media