Studies on the biosynthesis of epothilones: hydroxylation of Epo A and B to epothilones E and F.
When Sorangium cellulosum So ce90 is grown without XAD adsorber resin there is a steady state of epothilone A and B biosynthesis and hydroxylation of these products to epothilones E and F. This biotransformation at position C-21 of the thiazole ring is not restricted to producers of epothilones. It is carried out by a substrate induced monooxygenase. Epothilones E and F are further degraded by opening of the lactone ring by an esterase. Steps of degradation of different strains of S. cellulosum are compared.
Gerth
Klaus
K
GBF, Gesellschaft für Biotechnologische Forschung mbH, Abteilung Naturstoffbiologie, Braunschweig, Germany. kge@gbf.de
Steinmetz
Heinrich
H
Höflel
Gerhard
G
Reichenbach
Hans
H
D000970:Antineoplastic Agents / Q000378:metabolism*; D001711:Biotransformation; D034261:Epothilones; D006900:Hydroxylation; D018942:Macrolides / Q000378:metabolism*
D000970:Antineoplastic Agents; D034261:Epothilones; D018942:Macrolides; C093787:epothilone A; C093788:epothilone B