title: IL-15 regulation in human endometrial stromal cells.
abstract: A greater knowledge of IL-5 regulation within human endometrium is important in understanding key reproductive events and uterine Natural Killer cell function. In the present study, expression of IL-15 mRNA was shown to be up-regulated by both PGE(2) and IFN-gamma in cultures of human endometrial stromal cells (ESC). Release of IL-15 protein was also shown to be under the control of PGE(2) and IFN-gamma using an enzyme-linked immunosorbent assay for IL-15. In addition, 8-Bromo cAMP was able to increase IL-15 release from ESCs (P < 0.005) implying the actions of PGE(2) may be via this second messenger. Addition of a progestin appeared to enhance these effects. Real-time quantitative PCR has demonstrated an up-regulation in IL-15 mRNA expression in the late secretory phase of the menstrual cycle (P < 0.005) and a progressive rise in IFN-gamma expression throughout the secretory phase and into first trimester decidua. These results suggest that IL-15 regulation in the human endometrium is complex and that hormonal control may be indirect.
authors.affiliation: MRC Human Reproductive Sciences Unit, University of Edinburgh, Centre for Reproductive Biology, Edinburgh EH3 9ET, United Kingdom. c.dunn@hrsu.mrc.ac.uk
authors.forename: Carolyn L
authors.initials: CL
authors.lastname: Dunn
authors.forename: Hilary O D
authors.initials: HO
authors.lastname: Critchley
authors.forename: Rodney W
authors.initials: RW
authors.lastname: Kelly
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000328:Adult; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology; D004338:Drug Combinations; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism / Q000494:pharmacology; D019409:Interleukin-15 / Q000235:genetics / Q000378:metabolism*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D008597:Menstrual Cycle / Q000502:physiology; D045167:Progesterone Congeners; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D004338:Drug Combinations; D019409:Interleukin-15; D045167:Progesterone Congeners; D012333:RNA, Messenger; D015124:8-Bromo Cyclic Adenosine Monophosphate; D007371:Interferon-gamma; D017258:Medroxyprogesterone Acetate; D015232:Dinoprostone