title: TCP is hardly resorbed and not osteoconductive in a non-loading calvarial model.
abstract: Tricalciumphosphate (TCP) has been used as a ceramic bone substitute material in the orthopedic field as well as in craniofacial surgery. Some controversies exist concerning the osteoconductive potential of this material in different implantation sites. This study was designed to evaluate the biological response of calvarial bone towards TCP granules under non-loading conditions to assess the potential of TCP as a biodegredable and osteoconductive bone substitue material for the cranial vault. Full-thickness non-critical size defects were made bilaterally in the calvaria of 21 adult Wistar rats. One side was filled by TCP granules, the contralateral side was left empty and used as a control. Animals were sacrified in defined time intervals up to 6 months. Bone regeneration was analyzed with special respect toward the micromorphological and microanalytical features of the material-bone interaction by electron microscopy and electron diffraction analysis. Histologic examination revealed no TCP degradation even after 6 months of implantation. In contrast, a nearly complete bone regeneration of control defects was found after 6 months. At all times TCP was surrounded by a thin fibrous layer without presence of osteoblasts and features of regular mineralization. As far as degradation and substitution are concerned, TCP is a less favourable material tinder conditions of non-loading.
authors.affiliation: Biomineralization Reserach Unit, Westfälische Wilhelms-Universität Münster, Germany. handschelj@uni-muenster.de
authors.forename: Jörg
authors.initials: J
authors.lastname: Handschel
authors.forename: Hans Peter
authors.initials: HP
authors.lastname: Wiesmann
authors.forename: Udo
authors.initials: U
authors.lastname: Stratmann
authors.forename: Johannes
authors.initials: J
authors.lastname: Kleinheinz
authors.forename: Ulrich
authors.initials: U
authors.lastname: Meyer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Joos
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001862:Bone Resorption; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000033:anatomy & histology* / Q000737:chemistry; D013997:Time Factors; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C018392:tricalcium phosphate