title: Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan.
abstract: The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraperitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses > or =1 mg ml(-1). A single dose of 1mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at 1 day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the 1mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined.
authors.affiliation: Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB24 2TZ, Scotland, UK.
authors.forename: Scott
authors.initials: S
authors.lastname: Peddie
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Secombes
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003165:Complement System Proteins / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007958:Leukocyte Count; D009113:Muramidase / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage; D011480:Protease Inhibitors / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D000464:Alginates; D006603:Hexuronic Acids; D007375:Interleukin-1; D016209:Interleukin-8; D010936:Plant Extracts; D011480:Protease Inhibitors; D014409:Tumor Necrosis Factor-alpha; D020723:Glucuronic Acid; D003165:Complement System Proteins; D009113:Muramidase