Preliminary approach to elucidate the role of pigment as a binding site for drugs and chemicals in anagen hair: differential uptake of 3H-haloperidol by pigment-producing compared to non-pigment-producing cell lines.
A striking difference was observed for cellular-bound drug in HaCaT and Sk-Mel-1 cells for a fixed drug exposure time of 72 h and varying 3H-haloperidol concentrations in the culture media. Drug uptake was dependent on drug concentration and linearly correlated for both the non-pigment- and the pigment-producing cells which however was different in magnitude. In an additional investigation the time course of drug uptake during 3H-haloperidol exposure (400 pmol/ml; 28 days) revealed increasing drug concentrations in the Sk-Mel-1 population, whereas drug concentrations in the keratinocytes reached a plateau within a short time period. In contrast to the HaCaT cells no tendency to saturation was observed for the pigment-producing cell line. At the end of the experiments 3H-haloperidol concentrations in Sk-Mel-1 were found to be approximately tenfold higher than in HaCaT.
Pötsch
L
L
Institut für Rechtsmedizin, Johannes Gutenberg Universität, Mainz, Germany.
Emmerich
P
P
Skopp
G
G
D001665:Binding Sites; D002460:Cell Line; D006197:Hair / Q000378:metabolism*; D006220:Haloperidol / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008543:Melanins / Q000378:metabolism; D020460:Melanosomes / Q000378:metabolism*; D010858:Pigmentation; D014018:Tissue Distribution
D008543:Melanins; D006220:Haloperidol