Biodiversity (Communications arising): maize transgene results in Mexico are artefacts.
Quist and Chapela's conclusion that the transgenes they claim to have detected in native maize in Oaxaca, Mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-PCR) they used to amplify sequences flanking 35S transgenes from cauliflower mosaic virus (CaMV).
Kaplinsky
Nick
N
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA. nkaplins@nature.berkeley.edu
Braun
David
D
Lisch
Damon
D
Hay
Angela
A
Hake
Sarah
S
Freeling
Michael
M
D016477:Artifacts; D017931:DNA Primers / Q000235:genetics; D018745:Genome, Plant; D007438:Introns / Q000235:genetics; D008800:Mexico; D009693:Nucleic Acid Hybridization; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011995:Recombination, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D018626:Retroelements / Q000235:genetics*; D019076:Transgenes / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
D017931:DNA Primers; D018626:Retroelements