title: Effects of repeated short-term skin contact with proteolytic enzymes.
abstract: The objective was to investigate effects on the hand of protease used in dishwashing liquids (DWL). A group of 30 Koreans (normal skin) and 24 Japanese (12 atopic and 12 normal) were employed for two different studies, respectively. The 30 Koreans were divided into three groups. Each group immersed their hands for 15 min a day for 9 days into DWL containing 0.005% protease, 0.02% protease, or non-enzyme DWL. The 24 Japanese immersed their hands for 15 min a day for 4 days into 0.005% protease-containing or a non-enzyme DWL. The hand skin was evaluated by measuring overall and dry skin grades, estimated before and after exposures to the test products. The Korean study shows that 0.005% and 0.02% protease-containing DWL are less irritating to the skin than non-enzyme control, as measured by better overall skin grades and less dry skin. The Japanese study shows that 0.005% protease-containing DWL are less irritating to the skin than non-enzyme control in both atopic and normal subjects. The 0.005% protease use did not cause any adverse dermatological effects to atopic subjects compared with non-enzyme control. Both studies indicate that 0.005% and 0.02% protease resulted in better skin mildness profile, without causing adverse dermatological complications to the subjects.
authors.affiliation: The Procter & Gamble Far East, Kobe Technical Center, Japan. Lee.my@pg.com
authors.forename: Min Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Kyoung Sik
authors.initials: KS
authors.lastname: Park
authors.forename: Chizuru
authors.initials: C
authors.lastname: Hayashi
authors.forename: Hyung Hee
authors.initials: HH
authors.lastname: Lim
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Inshin
authors.initials: I
authors.lastname: Kwak
authors.forename: R Dana
authors.initials: RD
authors.lastname: Laurie
mesh_terms: D000704:Analysis of Variance; D016022:Case-Control Studies; D017449:Dermatitis, Allergic Contact / Q000209:etiology*; D003902:Detergents / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000009:adverse effects*; D006229:Hand Dermatoses / Q000139:chemically induced*; D006801:Humans; D013997:Time Factors
chemical_list: D003902:Detergents; D010450:Endopeptidases