Mild heat shock induces cyclin D1 synthesis through multiple Ras signal pathways.
Hyperthermia such as that occurring during fever may improve cell survival during infection, although its mechanism of action is largely unknown. Here we show that acute exposure to mild, but not severe, heat shock induces the synthesis of cyclin D1 that plays a critical role(s) in G1 progression of the cell cycle. This induction seemed to be regulated through multiple Ras signal pathways involving extracellular signal-regulated kinase, phosphatidylinositol 3-kinase, and Rac1/NADPH oxidase, all of which have well been documented to be responsible for growth factor-induced cyclin D1 expression. In a physiological sense, mild heat shock may regulate cell proliferation through inducing cyclin D1 along with growth factors.
Han
Song Iy
SI
Department of Molecular Biology, College of Natural Sciences, and Research Institute of Genetic Engineering, Pusan National University, 609-735, Pusan, South Korea.
Oh
Su Young
SY
Jeon
Won Je
WJ
Kim
Jung Mo
JM
Lee
Jun Hyuk
JH
Chung
Hae Young
HY
Choi
Yung Hyun
YH
Yoo
Mi Ae
MA
Kim
Han Do
HD
Kang
Ho Sung
HS
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D019938:Cyclin D1 / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005334:Fever / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018869:Heat-Shock Response / Q000502:physiology*; D006801:Humans; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism*
D004791:Enzyme Inhibitors; D019938:Cyclin D1; D019255:NADPH Oxidases; D019869:Phosphatidylinositol 3-Kinases; D020928:Mitogen-Activated Protein Kinases; D020830:rac1 GTP-Binding Protein; D018631:ras Proteins