Effects of different exposures of hyperbaric oxygen on ligament healing in rats.
Hyperbaric oxygen (HBO) is a method of augmenting, intermittently, oxygen availability to tissues. We examined the effect of three different HBO exposures on the healing of experimentally induced ligament lacerations in the right hind limb of 44 male Wistar rats. Animals were divided into four groups after ligament injury: (a) control group, animals breathed room air at 1 ATA (atmosphere absolute) in a hyperbaric chamber for 60 min; (b) HBO treatment at 1.5 ATA for 30 min once a day, (c) HBO treatment at 2 ATA for 30 min once a day, (d) 2 ATA for 60 min once a day. At 14 days post-ligament injury, we compared the ligaments of the four treatment groups for gross appearance, histology and expression of pro-alpha(I) mRNA by northern hybridization. Our results indicate that HBO was effective in promoting ligament healing compared to control (p < 0.01). Of these three exposures, HBO at 2 ATA for 60 min was the most effective, resulting in enhanced extra-cellular matrix deposition as measured by collagen synthesis.
Ishii
Yoshimasa
Y
Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan. yoshi.ishii@aist.go.jp
Ushida
Takashi
T
Tateishi
Tetsuya
T
Shimojo
Hitoshi
H
Miyanaga
Yutaka
Y
D000818:Animals; D015152:Blotting, Northern; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D004305:Dose-Response Relationship, Drug; D006614:Hindlimb / Q000378:metabolism / Q000473:pathology; D006931:Hyperbaric Oxygenation; D008023:Ligaments, Articular / Q000378:metabolism / Q000473:pathology*; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013708:Tendon Injuries / Q000628:therapy*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D011498:Protein Precursors; D012333:RNA, Messenger; D003094:Collagen