title: Complexin regulates the closure of the fusion pore during regulated vesicle exocytosis.
abstract: Membrane fusion during exocytosis and throughout the cell is believed to involve members of the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors) family of proteins. The assembly of these proteins into a four-helix bundle may be part of the driving force for bilayer fusion. Regulated exocytosis in neurons and related cell types is specialized to be fast and Ca(2+)-dependent suggesting the involvement of other regulatory proteins specific for regulated exocytosis. Among these are the complexins, two closely related proteins that bind only to the assembled SNARE complex. We have investigated the function of complexin by analysis of single vesicle release events in adrenal chromaffin cells using carbon fiber amperometry. These cells express complexin II, and overexpression of this protein modified the kinetics of vesicle release events so that their time course was shortened. This effect depended on complexin interaction with the SNARE complex as introduction of a mutation of Arg-59, a residue that interacts with synaptobrevin in the SNARE complex, abolished its effects. The data are consistent with a function for complexin in stabilizing an intermediate of the SNARE complex to allow kiss-and-run recycling of the exocytosed vesicle.
authors.affiliation: Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, United Kingdom.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Archer
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Graham
authors.forename: Robert D
authors.initials: RD
authors.lastname: Burgoyne
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D017931:DNA Primers; D005089:Exocytosis; D008561:Membrane Fusion / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D017931:DNA Primers; D009419:Nerve Tissue Proteins; C095993:complexin II