Investigation of antigen delivery route in vivo and imune-boosting effects mediated by pH-sensitive liposomes encapsulated with K(b)-restricted CTL epitope.
Using fluorescein isothiocyanate (FITC)-conjugated H-2K(b) CTL epitope (SIINFEKL) as a model system, we investigated the antigen delivery route by pH-sensitive liposomes in vivo. Fluorescence was initially detected in lymph nodes at 3 h after immunization, and its intensity reached a peak value in superticial inguinal lymph node at 9 h. No trace could be detected in spleen even with prolonged monitoring for up to 24 h. These results strongly suggest that the presentation of CTL-peptide antigen vehicled by pH-sensitive liposomes exclusively occurs in lymph nodes. In mice immunized with the H-2K(b) CTL epitope encapsulated pH-sensitive liposomes, peptide-specific CTL response was detected at day 3. The response was strongly augmented by the second immunization and persisted up to at least 45 days. These results suggest that pH-sensitive liposome formula functions as a potential adjuvant of peptide antigens and is useful for the induction of antigen specific CTLsv in vivo.
Lee
Ki-Young
KY
Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, 134 Shinchon-Dong, Seodaemun-Gu, Seoul, 120-749, South Korea
Chun
Eunyoung
E
Seong
Baik L
BL
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D004337:Drug Carriers; D004527:Egg Proteins / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006183:H-2 Antigens / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007114:Immunization / Q000379:methods; D008081:Liposomes; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010047:Ovalbumin / Q000276:immunology*; D010446:Peptide Fragments; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D004337:Drug Carriers; D004527:Egg Proteins; D018984:Epitopes, T-Lymphocyte; D005456:Fluorescent Dyes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D008081:Liposomes; C110366:OVA-8; D010446:Peptide Fragments; D010455:Peptides; D010047:Ovalbumin; D016650:Fluorescein-5-isothiocyanate