title: Survival of Cryptosporidium parvum oocysts recovered from experimentally contaminated oysters (Ostrea edulis) and clams (Tapes decussatus).
abstract: Samples of two species of shellfish that form part of the human food chain (the oyster Ostrea edulis and the marine clam Tapes decussatus) were experimentally contaminated with Cryptosporidium parvum oocysts. Changes in the viability of oocysts subsequently recovered from the shellfish were evaluated by means of an immunofluorescent antibody technique (IFAT) and inclusion/exclusion of the fluorogenic vital dye propidium iodide. There was a sharp decrease in oocyst viability during the first 4 days, with 15-25% viable oocysts remaining thereafter. In addition the infectivity of these oocysts at 10 and 31 days post-contamination was demonstrated using a suckling murine model.
authors.affiliation: Departamento de Microbiologia y Parasitologia, Universidad de Santiago de Compostela, Coruña, Spain
authors.forename: F
authors.initials: F
authors.lastname: Freire-Santos
authors.forename: H
authors.initials: H
authors.lastname: Gómez-Couso
authors.forename: M R
authors.initials: MR
authors.lastname: Ortega-Iñarrea
authors.forename: J A
authors.initials: JA
authors.lastname: Castro-Hermida
authors.forename: A M
authors.initials: AM
authors.lastname: Oteiza-López
authors.forename: O
authors.initials: O
authors.lastname: García-Martín
authors.forename: M E
authors.initials: ME
authors.lastname: Ares-Mazás
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology*; D002417:Cattle; D016785:Cryptosporidium parvum / Q000302:isolation & purification* / Q000472:pathogenicity; D020387:Food Chain; D006790:Host-Parasite Interactions; D051379:Mice; D033661:Oocysts / Q000254:growth & development; D010125:Ostreidae / Q000469:parasitology*; D010270:Parasite Egg Count; D014871:Water Microbiology