title: Glutathione peroxidase from the liver of Japanese sea bass Lateolabrax japonicus.
abstract: Glutathione peroxidase (EC 1.11.1.9) present in the liver of Japanese sea bass (Lateolabrax japonicus) was extracted and purified by phenyl-toyopearl 650M, butyl-toyopearl 650M and DEAE-toyopearl 650M column chromatography. The molecular weight of the enzyme was estimated to be about 70 kDa by gel filtration by toyopearl HW-55F. On SDS-PAGE, this enzyme was composed of two identical subunits with 35 kDa and was a dimer. This enzyme was a typical SH-enzyme that was inhibited by iodoacetic acid, PCMB, DTNB, and Hg.
authors.affiliation: Department of Food Science and Technology, National Fisheries University, Shimonoseki, Yamaguchi, Japan. machin@fish-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagai
authors.forename: Takakiyo
authors.initials: T
authors.lastname: Yukimoto
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001492:Bass; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005979:Glutathione Peroxidase / Q000302:isolation & purification / Q000378:metabolism*; D007564:Japan; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D013816:Thermodynamics
chemical_list: D005979:Glutathione Peroxidase