High prevalence of cfxA beta-lactamase in aminopenicillin-resistant Prevotella strains isolated from periodontal pockets.
This prospective study was designed to investigate amoxicillin-resistant oral anaerobes, and to identify their beta-lactamase-encoding genes. Three subgingival bacterial samples were collected from 12 patients suffering from periodontitis. One to seven beta-lactamase-producing strains were obtained from each patient, mostly belonging to the Prevotella genus (Bacteroides eggerthii, 2/35 strains; Prevotella sp., 33/35 strains). PCR assays were used to detect cfxA and cepA/cblA, the genes encoding class A/group2e beta-lactamases previously described in the Bacteroides fragilis group. The present investigation confirmed the role of Prevotella species as beta-lactamase producers in periodontal pockets. Additionally, this PCR screening showed (1): the high prevalence of CfxA beta-lactamase production by aminopenicillin-resistant Prevotella (32/33: 97.0% positive strains) vs. cepA/cblA (1/33: 3.0% positive strains), and (2) the presence of cfxA in the periodontal reservoir in the absence of antimicrobial therapy during the previous 6 months.
Fosse
T
T
Laboratoire de Bactériologie, Hôpital l'Archet 2, Centre Hospitalier Universitaire de Nice, Nice, France.
Madinier
I
I
Hannoun
L
L
Giraud-Morin
C
C
Hitzig
C
C
Charbit
Y
Y
Ourang
S
S
D000658:Amoxicillin / Q000494:pharmacology; D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004359:Drug Therapy, Combination / Q000494:pharmacology; D004917:Erythromycin / Q000494:pharmacology; D036781:Fimbriae Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D010403:Penicillin Resistance / Q000235:genetics*; D010406:Penicillins / Q000494:pharmacology; D010514:Periodontal Pocket / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018720:Prevotella / Q000145:classification / Q000235:genetics*; D011446:Prospective Studies; D013752:Tetracycline / Q000494:pharmacology; D013753:Tetracycline Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D008565:Membrane Proteins; D010406:Penicillins; D036781:Fimbriae Proteins; D004917:Erythromycin; D019980:Amoxicillin-Potassium Clavulanate Combination; D000658:Amoxicillin; C462979:cfxA protein, Prevotella; D001618:beta-Lactamases; D013752:Tetracycline