Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan.
The present work provides information concerning the immunostimulatory activity of Ergosan, an algal based product, injected intraperitoneally in the rainbow trout (Oncorhynchus mykiss). Ergosan is composed of 0.002% unspecified plant extract, 1% alginic acid from Laminaria digitata, and 98.998% algal based carrier. Migration of leucocytes into the peritoneal cavity was stimulated at doses > or =1 mg ml(-1). A single dose of 1mg significantly augmented the proportion of neutrophils, degree of phagocytosis, respiratory burst activity and expression of interleukin-1beta (IL-1beta), interleukin-8 (IL-8) and one of the two known isoforms of trout tumour necrosis factor-alpha (TNF2) in peritoneal leucocytes at 1 day post-injection. Humoral immune parameters were less responsive to intraperitoneal Ergosan administration, with complement stimulation only evident in the 1mg treated group at 2 days post-injection. Antiprotease and lysozyme activity were unaffected by Ergosan over a 7-day time period at the doses examined.
Peddie
Scott
S
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB24 2TZ, Scotland, UK.
Zou
Jun
J
Secombes
Christopher J
CJ
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000464:Alginates / Q000008:administration & dosage*; D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003165:Complement System Proteins / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000235:genetics; D016209:Interleukin-8 / Q000235:genetics; D007958:Leukocyte Count; D009113:Muramidase / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage; D011480:Protease Inhibitors / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D000276:Adjuvants, Immunologic; D000464:Alginates; D006603:Hexuronic Acids; D007375:Interleukin-1; D016209:Interleukin-8; D010936:Plant Extracts; D011480:Protease Inhibitors; D014409:Tumor Necrosis Factor-alpha; D020723:Glucuronic Acid; D003165:Complement System Proteins; D009113:Muramidase