Improved cell growth in tobacco suspension cultures expressing Vitreoscilla hemoglobin.
Expression of the gene encoding bacterial hemoglobin (VHb) from Vitreoscilla has been previously used to improve recombinant cell growth and enhance product formation under microaerobic conditions, a common phenomenon in large-scale cultivations of bacteria. This technology has now been applied to tobacco suspension cultures. Tobacco suspension cultures have been generated from VHb-expressing tobacco plants. Cell cultures were capable of producing an active hemoglobin. When grown in shake flasks, the cells did not show any lag-phase and exhibited improved cell growth, compared to controls carrying the parental plasmid.
Farrés
Judith
J
Institute of Biotechnology, ETH-Zürich, CH-8093, Switzerland.
Kallio
Pauli T
PT
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D046508:Culture Techniques; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005240:Feasibility Studies; D005285:Fermentation; D006454:Hemoglobins / Q000235:genetics* / Q000378:metabolism; D018527:Hydroponics; D018515:Plant Leaves / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D000431:Ethanol; D013395:Sucrose