title: [The remineralization of enamel lesions by casein phosphopeptide-amorphous calcium fluoride phosphate in vitro].
abstract: OBJECTIVE
  To evaluate the remineralizing effect on artificial enamel subsurface lesions in vitro by 2% casein phosphopeptide-amorphous calcium fluoride phosphate complexes (CPP-ACFP) solution.
  
  
  METHODS
  Twenty-eight enamel blocks were cut from human third molars, in which 2 lesions were prepared from each block. The 28 samples were randomly assigned to the four study groups, in which each group was incubated in remineralization solution for 1, 3, 5, 10 days respectively. After remineralization the samples were sectioned, microradiographied and the mineral content were determined by microdensitometry.
  
  
  RESULTS
  The remineralizing solution replaced 9.19%, 14.27%, 29.07%, 38.45% of mineral lost after 1, 3, 5, 10 days respectively (y = 8.9316 x0.6347, R2 = 0.9322; y: remineralizing rate, x: time). Significant differences were found in remineralizing rate between any two groups using One-way Scheffe multiple comparison.
  
  
  CONCLUSIONS
  The 2% CPP-ACFP solution could significantly remineralize the artificial enamel subsurface lesion in vitro. The remineralizing rates were increased with the experiment time (within 10 days).
authors.affiliation: Department of Stomatology, First Affiliated Hospital of Medical College, University of Shantou, Guangdong 515041, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: F
authors.initials: F
authors.lastname: Cai
mesh_terms: D002364:Caseins / Q000494:pharmacology*; D003743:Dental Enamel / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D013997:Time Factors; D014074:Tooth Calcification / Q000187:drug effects*
chemical_list: D002364:Caseins; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex