Association between AAAG repeat polymorphism in the P3 promoter of the human parathyroid hormone (PTH)/PTH-related peptide receptor gene and adult height, urinary pyridinoline excretion, and promoter activity.
The PTH/PTHrP receptor (PTHR1) plays an essential role in skeletal development and mediates many other functions of PTH and PTHrP. Human PTHR1 gene transcription is controlled by three promoters, P1-P3. The most proximal promoter, P3, is active in bone and osteoblast-like cell lines and accounts for the majority of renal transcripts in adults. We have identified a tetranucleotide repeat (AAAG)n polymorphism in the P3 promoter. In 214 unrelated Japanese, the repeat number (n) ranged from 3-8, with the AAAG5 allele being the most frequent (59%). In 55 unrelated Caucasians, n ranged from 5-7, and the frequency of the AAAG5 allele was 78%. The most frequent genotypes in a cohort of 85 young (18-20 yr) female Japanese were 5/5, 5/6, and 6/6. The 6/6 genotype was associated with greater height (5/5 vs. 6/6; P < 0.02) and lower urinary deoxypyridinoline and pyridinoline (P < 0.02), which are markers of bone resorption. The height of an additional 71 healthy female Japanese subjects, aged 14-17 yr, having genotype 5/5, 5/6, or 6/6 was also in the order of genotype 5/5 < 5/6 < 6/6 (5/5 vs. 6/6, P < 0.05). There was no significant difference in lumbar and femoral bone mineral density between genotypes. Likewise, there was no difference in circulating intact PTH levels between groups. The activity of P3 promoter-luciferase reporter constructs in transcription assays in 2 human osteoblast-like cell-lines varied according to repeat number, with AAAG6 being the least active. In conclusion, the P3 promoter (AAAG)n polymorphism is frequent in both Japanese and Caucasians and has potential as a linkage marker for the PTHR1 locus. In addition, it may influence the expression of the receptor in target tissues and have functional consequences on the developing skeleton.
Minagawa
Masanori
M
Department of Pediatrics, Chiba University Graduate School of Medicine, Inohana, Chuo-ku, Chiba 260-8670, Japan.
Yasuda
Toshiyuki
T
Watanabe
Tomoyuki
T
Minamitani
Kanshi
K
Takahashi
Yoshihito
Y
Goltzman
David
D
White
John H
JH
Hendy
Geoffrey N
GN
Kohno
Yoichi
Y
D000328:Adult; D000596:Amino Acids / Q000652:urine*; D000886:Anthropometry; D044466:Asian People / Q000235:genetics; D015415:Biomarkers; D001769:Blood / Q000378:metabolism; D001827:Body Height / Q000235:genetics*; D015519:Bone Density; D001862:Bone Resorption / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan; D011110:Polymorphism, Genetic / Q000235:genetics* / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D044168:Receptor, Parathyroid Hormone, Type 1; D018016:Receptors, Parathyroid Hormone / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D044465:White People / Q000235:genetics
D000596:Amino Acids; D015415:Biomarkers; D044168:Receptor, Parathyroid Hormone, Type 1; D018016:Receptors, Parathyroid Hormone; C015484:pyridinoline