Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range.
We report the development of simultaneous two-color channel recording in 4Pi-confocal microscopy. A marked increase of spatial resolution over confocal microscopy becomes manifested in 4Pi-confocal three-dimensional (3D) data stacks of dual-labeled objects. The fundamentally improved resolution is verified both with densely labeled fluorescence beads as well as with membrane labeled fixed Escherichia coli. The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range.
Kano
H
H
High Resolution Optical Microscopy Group, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Jakobs
S
S
Nagorni
M
M
Hell
S W
SW
D002462:Cell Membrane; D004926:Escherichia coli / Q000166:cytology; D005456:Fluorescent Dyes; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008863:Microspheres; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
D005456:Fluorescent Dyes