Cloning and sequencing of the 3'-region of the canine parathyroid hormone-related protein gene and analysis of alternate mRNA splicing in two canine carcinomas.
A canine genomic library in Lambda FIX II vector was screened with a 281-base pair canine PTHrP cDNA to the prepro- and coding regions. Two genomic clones were isolated and mapped to the 3'-end of the PTHrP gene by polymerase chain reaction (PCR) amplification of exons in this region. One clone (3.5 kb) was amplified by PCR, partially sequenced, and compared to the human PTHrP gene. Regions were identified with a high degree of homology to exons 6, 7, and 8 of the human PTHrP gene. A polyadenylation site was present 3' to the exon 8-like region. Reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated that exon 7 of the PTHrP gene was transcribed in two canine carcinomas (SCC 2/88 cells and CAC-8 tumor line) which produce PTHrP. This confirmed that the 3'-region of the canine PTHrP gene is alternately spliced with splicing of exon 6 to exons 7 or 9. Transcription of exon 8 was not demonstrated by RT-PCR and suggests that the exon 8-like region of the dog PTHrP gene is not utilized. The exon 8-like region contained an early stop codon that was not present in exon 8 of the human PTHrP gene.
Gröne
A
A
Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA.
McCauley
L K
LK
Capen
C C
CC
Rosol
T J
TJ
D000230:Adenocarcinoma / Q000235:genetics / Q000662:veterinary; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001050:Apocrine Glands; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000662:veterinary; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D015723:Gene Library; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000662:veterinary*; D044162:Parathyroid Hormone-Related Protein; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013544:Sweat Gland Neoplasms / Q000235:genetics / Q000662:veterinary; D014407:Tumor Cells, Cultured
D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm