The farnesyl transferase inhibitor RPR-130401 does not alter radiation susceptibility in human tumor cells with a K-Ras mutation in spite of large changes in ploidy and lamin B distribution.
BACKGROUND
Growth inhibition by RPR-130401, a non-peptidomimetic farnesyltransferase inhibitor, was investigated without or with combined exposure to ionizing radiation in three human tumor cell lines (HCT-116, MiAPaCa-2 and A-549) bearing a point mutation in the K-Ras gene.


RESULTS
RPR-130401 inhibited cell growth with an IC50 of 50 nM (HCT-116), 120 nM (MiAPaCa-2) and 710 nM (A-549), with a poor incidence of apoptosis. The drug brought about G1 and S phase depletion together with arrest of cells in G2 phase and induced a significant accumulation of hyperploid cells showing active S phase DNA synthesis, with HCT-116 and A-549 cells being the most and least responsive, respectively. The drug also produced dramatic changes of the nuclear lamin B pattern, without lamin B cleavage and perturbation of the actin cytoskeleton. On the other hand, RPR-130401 elicited strictly additive interaction in combined treatment with ionizing radiation with regard to cell kill, altered cell cycle progression and induced hyperploidy.


CONCLUSIONS
The data suggest that disruption of orderly progression through mitosis and cytokinesis, is a major outcome of drug action and that this effect proceeds from inhibition of lamin B farnesylation. It is anticipated from the strict additivity of RPR-130401 and radiation that neither induced radiation resistance nor acute or late complications of radiotherapy, should occur in combined treatment with RPR-130401.
Mégnin-Chanet
Frédérique
F
U 350 INSERM, Institut Curie-Recherche, Centre Universitaire, Orsay, France. frederique.megnin@curie.u-psud.fr
Lavelle
François
F
Favaudon
Vincent
V
D000199:Actins / Q000378:metabolism; D019883:Alkyl and Aryl Transferases / Q000037:antagonists & inhibitors*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D051231:Farnesyltranstransferase; D011905:Genes, ras; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D007621:Karyotyping; D034921:Lamin Type B / Q000378:metabolism*; D009154:Mutation; D011003:Ploidies; D011827:Radiation; D011836:Radiation Tolerance / Q000187:drug effects*; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D007211:Indoles; D034921:Lamin Type B; C400543:RPR 130401; D014404:Tubulin; D019883:Alkyl and Aryl Transferases; D051231:Farnesyltranstransferase