Aqueous oxygen hyperbaric reperfusion in a porcine model of myocardial infarction.
OBJECTIVE
The purpose of the study was to test the hypothesis that intracoronary aqueous oxygen (AO) hyperbaric reperfusion reduces myocardial injury after prolonged coronary occlusion. Background. Attenuation of ischemia/reperfusion injury by the use of hyperbaric oxygen (HBO) administered during reperfusion has been demonstrated for a wide variety of tissues, including myocardium. We have recently developed a more practical, catheter-based, site-specific method for delivery of oxygen at hyperbaric levels with aqueous oxygen infusion.


METHODS
Following a 60-minute balloon occlusion of the left anterior descending coronary artery in swine, intracoronary AO hyperoxemic perfusion (50 mL blood/minute; 1.5 mL AO/minute; mean pO2 = 834   104 mmHg) was performed for 90 minutes after a 15-minute period of normoxemic autoreperfusion (physiologic reperfusion). Control groups consisted of autoreperfusion alone; active normoxemic perfusion (50 mL/minute) for 90 minutes; and hyperoxemic perfusion with a hollow fiber oxygenator (HFO) for 90 minutes. Results. A significant improvement in left ventricular ejection fraction was noted by ventriculography at 105 minutes of reperfusion (ANOVA, p < 0.05), compared to the 15-minute autoreperfusion period, only in the AO and HFO groups. Mean percent infarct size (area of necrosis)/(area at risk), quantitative post-mortem hemorrhage score, and myocardial myeloperoxidase levels at 3 hours of reperfusion were significantly less in the AO group (ANOVA, p < 0.05), but not in the HFO group, compared to normoxemic groups. Conclusions. The results demonstrate that intracoronary hyperbaric reperfusion with AO, but not with a membrane oxygenator, attenuates myocardial ischemia/reperfusion injury.
Spears
J Richard
JR
Cardiovascular Research Laboratory, Wayne State University, Detroit, MI 48201, USA. aa1811@wayne.edu
Henney
Cassandra
C
Prcevski
Petar
P
Xu
Rui
R
Li
Li
L
Brereton
Giles J
GJ
DiCarli
Marcello
M
Spanta
Ali
A
Crilly
Richard
R
Sulaiman
Abdulbaset M
AM
Hadeed
Samir
S
Lavine
Steven
S
Patterson
William R
WR
Creech
Jeffrey
J
Vander Heide
Richard
R
D000704:Analysis of Variance; D000818:Animals; D006931:Hyperbaric Oxygenation / Q000379:methods*; D007261:Infusions, Intra-Arterial; D023421:Models, Animal; D009203:Myocardial Infarction / Q000628:therapy*; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D013552:Swine