Antitumor activity of compounds isolated from leaves of Eriobotrya japonica.
In a search for possible antitumor agents from natural sources, megastigmane glycosides and polyphenolic constituents isolated from the leaves of Eriobotrya japonica (Rosaceae) were found to inhibit the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced activation of Epstein-Barr virus early antigen in Raji cells. Roseoside and procyanidin B-2 were among the active compounds found in an in vitro assay; these compounds were further assessed for antitumor activity in vivo in a two-stage carcinogenesis assay on mouse skin. Roseoside significantly delayed carcinogenesis induced by peroxynitrite (initiator) and TPA (promoter), and its potency was comparable to that of a green tea polyphenol, (-)-epigallocatechin 3-O-gallate, in the same assay.
Ito
Hideyuki
H
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530, Japan.
Kobayashi
Eri
E
Li
Shu-Hua
SH
Hatano
Tsutomu
T
Sugita
Daigo
D
Kubo
Naoki
N
Shimura
Susumu
S
Itoh
Yoshio
Y
Tokuda
Harukuni
H
Nishino
Hoyoku
H
Yoshida
Takashi
T
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000627:therapeutic use*; D044946:Biflavonoids; D002273:Carcinogens; D002392:Catechin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000627:therapeutic use; D005260:Female; D005960:Glucosides / Q000302:isolation & purification / Q000627:therapeutic use; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D009303:Nasopharyngeal Neoplasms / Q000821:virology; D045792:Norisoprenoids; D030421:Peroxynitrous Acid; D018515:Plant Leaves / Q000737:chemistry*; D044945:Proanthocyanidins; D027824:Rosaceae / Q000737:chemistry*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy; D013662:Tea / Q000737:chemistry; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014775:Virus Activation / Q000187:drug effects
D000956:Antigens, Viral; D000970:Antineoplastic Agents; D044946:Biflavonoids; D002273:Carcinogens; D005960:Glucosides; D045792:Norisoprenoids; D044945:Proanthocyanidins; D013662:Tea; C438027:roseoside; D030421:Peroxynitrous Acid; C479578:procyanidin B; C017674:procyanidin; D015127:9,10-Dimethyl-1,2-benzanthracene; D002392:Catechin; C045651:epigallocatechin gallate; D013755:Tetradecanoylphorbol Acetate