title: Urea-induced denaturation of human serum albumin labeled with acrylodan.
abstract: We induced the denaturation of unlabeled human serum albumin (HSA) and of similar albumin labeled with acrylodan (6-acryloyl-2-dimethylamino naphthalene) with urea and studied the transition profiles using circular dichroism and fluorescence spectroscopy. The circular dichroism spectra for both albumin preparations resulted in the same curves, thus indicating that labeling with acrylodan does not perturb the conformation of HSA. Our results indicate that the denaturation of both albumin preparations takes place at a single, two-state transition with midpoint at about 6 M urea, due to the unfolding of its domain II. It is important to point out that even at 8 M urea, some residual structure remains in the HSA. Great changes in the fluorescence of the dye bound to the protein were observed by addition of solid guanidine hydrochloride to the protein labeled with acrylodan dissolved in 8 M urea, indicating that domain I of this protein was not denatured by urea.
authors.affiliation: Unidad de RMN, Dpto de Química-Física Farmacéutica, Facultad de Farmacia, Madrid. jglezjne@pluri.ucm.es
authors.forename: José
authors.initials: J
authors.lastname: González-Jiménez
authors.forename: Manuel
authors.initials: M
authors.lastname: Cortijo
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives*; D002942:Circular Dichroism; D005456:Fluorescent Dyes; D006801:Humans; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry* / Q000187:drug effects; D014508:Urea / Q000494:pharmacology*
chemical_list: D005456:Fluorescent Dyes; D012709:Serum Albumin; C038518:acrylodan; D014508:Urea; D015081:2-Naphthylamine