Tandem mass spectra of tryptic peptides at signal-to-background ratios approaching unity using electrospray ionization high-field asymmetric waveform ion mobility spectrometry/hybrid quadrupole time-of-flight mass spectrometry.
High-field asymmetric waveform ion mobility spectrometry (FAIMS) has been coupled to a quadrupole time-of-flight mass spectrometer for the tandem mass spectrometric analysis of tryptic peptides of pig hemoglobin. Using FAIMS, low levels (fmol/microL) of multiply charged tryptic peptides were separated from relatively intense chemical background such that their tandem mass spectra (MS/MS) lacked many background-related fragment ions observed using a conventional ESI-QqTOFMS instrument. Substantial improvements in both first-order and tandem mass spectra were realized while maintaining approximately the same absolute intensities.
Barnett
David A
DA
Ionalytics Corporation, Bldg M50-IPF, 1200 Montreal Road, Ottawa, Canada, K1A 0R6.
Ding
Luyi
L
Ells
Barbara
B
Purves
Randy W
RW
Guevremont
Roger
R
D000818:Animals; D006454:Hemoglobins / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*; D013552:Swine; D014357:Trypsin / Q000737:chemistry*
D006454:Hemoglobins; D010455:Peptides; D014357:Trypsin