Influence of hyperosmotic shrinkage and beta-adrenergic stimulation on red blood cell volume regulation and oxygen binding properties in rainbow trout and carp.
Whole blood from rainbow trout and carp was subjected to hyperosmotic shock and subsequent beta-adrenergic stimulation (isoprenaline) at different oxygen tension ( PO(2)) and carbon dioxide tension ( PCO(2)) levels with the aim to evaluate changes in red blood cell (RBC) volume, pH and ion concentrations and their ultimate effect on blood O(2) transport characteristics. Hyperosmolality (addition of NaCl) induced RBC shrinkage, which was followed by a regulatory volume increase (RVI) that was larger at low than at high PO(2)and more complete in carp than in trout. Carp RBC showed practically full volume recovery within 140 min at low PO(2)and partial recovery at high PO(2), whereas RVI was partial under all PO(2)and PCO(2)conditions in trout. The RVI increased intracellular [Na(+)], water content, and, in carp, also pH (pHi), suggesting activation of Na(+)/H(+) exchange. In trout RBCs, activation of RVI was rapid but succeeded by deactivation. In carp RBCs, activation of Na(+) influx was slower but it continued, allowing full volume recovery. Shrinkage of the RBCs was associated with only minor decreases in blood oxygen saturation and oxygen affinity in both species. Thus, the oxygen affinity decrease expected on the basis of the increased concentration of intracellular haemoglobin and organic phosphates was small, and it appeared to some extent countered during RVI by an oxygen affinity increase via increased pHi. Addition of isoprenaline increased RBC volume and pHi and increased Hb oxygen saturation. The beta-adrenergic response was stronger at low compared to high PO(2) and at high compared to low PCO(2). The PO(2) dependency was largest in carp, whereas the PCO(2) (pH) dependency was more expressed in trout. The adrenergic response of trout RBCs was similar under isoosmotic and hyperosmotic conditions. In carp RBCs, the response was absent at high PO(2) under isoosmotic conditions, but interestingly it could be induced under hyperosmotic conditions. The data suggest that the RBC shrinkage occurring in fish moving from freshwater to seawater has minimal impact on blood O(2) binding properties.
Brauner
C J
CJ
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA.
Wang
T
T
Jensen
F B
FB
D000222:Adaptation, Physiological / Q000502:physiology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D002347:Carps; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D005618:Fresh Water; D007545:Isoproterenol / Q000494:pharmacology*; D019344:Lactic Acid / Q000097:blood; D009711:Nucleotides / Q000097:blood; D017686:Oncorhynchus mykiss; D009997:Osmotic Pressure; D010100:Oxygen / Q000097:blood*; D012623:Seawater; D012965:Sodium Chloride / Q000097:blood; D013045:Species Specificity; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
D000318:Adrenergic beta-Agonists; D009711:Nucleotides; D019344:Lactic Acid; D012965:Sodium Chloride; D007545:Isoproterenol; D010100:Oxygen