Scopolamine impairs eyeblink conditioning in cerebellar LTD-deficient mice.
We investigated the effect of the muscarinic acetylcholine receptor (mAChR) antagonist scopolamine on eyeblink conditioning in glutamate receptor subunit 62 null mice, which have severe impairments in cerebellar long-term depression (LTD). Mice were injected intraperitoneally with scopolamine (0.5 mg/kg) or saline, and conditioned using a delay paradigm with tone and periorbital shock but with no overlap between them. The saline-injected mutant mice learned this paradigm normally, as predicted from our previous study. When scopolamine was injected, learning was impaired more severely in the mutant mice than in the wild type mice. Basic sensory and motor performances were not affected. These results suggest that eyeblink conditioning in cerebellar LTD deficient mice depends largely on neural functions susceptible to blocking of mAChRs.
Takatsuki
Kanako
K
Laboratory of Neurobiophysics, School of Pharmaceutical Science, School of Medicine, The University of Tokyo, Japan.
Kawahara
Shigenori
S
Mori
Hisashi
H
Mishina
Masayoshi
M
Kirino
Yutaka
Y
D000818:Animals; D001767:Blinking / Q000502:physiology*; D002531:Cerebellum / Q000502:physiology*; D003213:Conditioning, Psychological / Q000187:drug effects*; D007858:Learning / Q000187:drug effects / Q000502:physiology; D017774:Long-Term Potentiation / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout / Q000235:genetics; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D017470:Receptors, Glutamate / Q000235:genetics / Q000502:physiology; D011976:Receptors, Muscarinic / Q000502:physiology; D012016:Reference Values; D012601:Scopolamine / Q000494:pharmacology*
D018727:Muscarinic Antagonists; D017470:Receptors, Glutamate; D011976:Receptors, Muscarinic; C107913:glutamate receptor delta 2; D012601:Scopolamine