[The effects of butyrates and non-steroidal anti-inflammatory agents on HT-29 colonic carcinoma cells].
OBJECTIVE
Adenocarcinoma cells HT-29 were used to investigate whether butyrates and non-steroidal anti-inflammatory agents (NSAIDs, aspirin and NS-398) would be effective adjuvants for inducing apoptosis and to probe its possible mechanisms.


METHODS
Prostaglandin E(2) (PGE(2)) isolated from HT-29 cell culture supernants was investigated with ELISA. Flow cytometry was used for estimating apoptosis. The morphology of apoptotic cells was investigated by means of electron microscopy.


RESULTS
Butyrates could stimulate the secretion of PGE(2). In contrast, NSAIDs inhibited it to a level below 30 ng/L. Both butyrates and NSAIDs could induce apoptosis. The effects showed the obvious tendency of time- and concentration-dependency (P < 0.05). Combination with two drugs could enhance the above effects.


CONCLUSIONS
Butyrates and NSAIDs could induce apoptosis individually. Combination with the two drugs could enhance the above effects by down-regulating cyclooxygenase-2 expression.
Zhang
Zhihong
Z
Department of Gastroenterology, The First Affiliated Hospital, Western China University of Medical Sciences, Chengdu 610041, China.
Ouyang
Qin
Q
Gan
Huatian
H
D000230:Adenocarcinoma; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis; D001241:Aspirin / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology*; D003110:Colonic Neoplasms; D015232:Dinoprostone / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods; D019073:HT29 Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009578:Nitrobenzenes / Q000494:pharmacology*; D013449:Sulfonamides / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002087:Butyrates; D009578:Nitrobenzenes; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide; D015232:Dinoprostone; D001241:Aspirin