Iodine species and the endocrine system: thyroid hormone levels in adult Danio rerio and developing Xenopus laevis.
Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS). This method enables quantification of iodide, T4 and T3, as well as reverse T3 (rT3) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the LC-ICP-MS approach was used to analyze whole-body homogenates of adult male and female zebrafish (Danio rerio) and tadpoles of the African clawed frog (Xenopus laevis) at two different developmental stages (NF58 and 61) according to Nieuwkoop and Faber. The data demonstrate that the LC-ICP-MS method was successful at measuring I-, MIT, DIT, T4, T3, and rT3 in these two species. Furthermore, the method also detected five additional iodinated compounds which are currently unidentified.
Simon
R
R
GSF--Institute of Ecological Chemistry, Neuherberg, Germany. rsimon@gsf.de
Tietge
J E
JE
Michalke
B
B
Degitz
S
S
Schramm
K W
KW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D017613:Iodine Compounds / Q000032:analysis / Q000378:metabolism; D008297:Male; D013961:Thyroid Gland / Q000737:chemistry* / Q000378:metabolism; D013963:Thyroid Hormones / Q000032:analysis* / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
D017613:Iodine Compounds; D013963:Thyroid Hormones