Immunohistochemical detection and distribution of inducible nitric oxide synthase in pigs naturally infected with Actinobacillus pleuropneumoniae.
Inducible nitric oxide synthase (iNOS) protein was detected immunohistochemically in formalin-fixed, paraffin wax-embedded lung tissues from 10 natural cases of porcine pleuropneumonia. Positive cells typically exhibited a red reaction product without background staining. Labelling of iNOS protein was intense in "oat cells", the clustered leucocytes with streaming nuclear chromatin that are a characteristic histological feature of porcine pleuropneumonia. Macrophages and neutrophils within alveolar spaces but not within blood vessels consistently showed iNOS labelling, but such labelling was minimal in non-lesional lung of Actinobacillus pleuropneumoniae -infected pigs and in normal lung from control pigs. The results suggest that iNOS plays a role in pathophysiological processes during A. pleuropneumoniae infection.
Cho
W-S
WS
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
Chae
C
C
D000189:Actinobacillus Infections / Q000201:enzymology / Q000473:pathology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary*; D008168:Lung / Q000201:enzymology / Q000382:microbiology / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology* / Q000382:microbiology / Q000473:pathology
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II