[Effect of interleukin 2 on the expression of estrogen receptors in the anterior pituitary of rats].
Using primary serum-free cell culture combined with immunocytochemistry and semi-quantified RT-PCR methods, we observe the modulation of estrogen receptor alpha (ERalpha) and beta (ERbeta) by interleukin-2 (IL-2) in rat anterior pituitary. The results show that IL-2 up-regulates the level of ERalpha protein and the expression of ERalpha mRNA, but down-regulates those of ERbeta. The density per cell of ERalpha-immunoreactive (ir) cells increases from 48.740 4.567 to 81.188+/-6.619, whereas that of ERbeta -ir cells decreases from 102.560+/-6.250 to 72.718+/-7.623 after rhIL-2 (10 microgram/L) incubation for 48 h. In parallel with these changes, the ratio of ERalpha/ beta -actin mRNA increases from 0.1511 to 0.4334, and ERbeta /beta -actin mRNA declines from 0.3822 to 0.1528. It is likely that IL-2 has direct regulatory effect on ER in anterior pituitary.
Zhang
Q H
QH
Department of Physiology, The Fourth Military Medical University, Xi an 710032, China.
Zhu
Y L
YL
Hu
Y Z
YZ
Zhang
W H
WH
Chen
J K
JK
Wang
F Z
FZ
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction
D007376:Interleukin-2; D012333:RNA, Messenger; D011960:Receptors, Estrogen