title: The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress.
abstract: The cellular response to environmental changes includes widespread modifications in gene expression. Here we report the identification and characterization of Rsc9, a member of the RSC chromatin-remodeling complex in yeast. The genome-wide localization of Rsc9 indicated a relationship between genes targeted by Rsc9 and genes regulated by stress; treatment with hydrogen peroxide or rapamycin, which inhibits TOR signaling, resulted in genome-wide changes in Rsc9 occupancy. We further show that Rsc9 is involved in both repression and activation of mRNAs regulated by TOR as well as the synthesis of rRNA. Our results illustrate the response of a chromatin-remodeling factor to signaling cascades and suggest that changes in the activity of chromatin-remodeling factors are reflected in changes in their localization in the genome.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Damelin
authors.forename: Itamar
authors.initials: I
authors.lastname: Simon
authors.forename: Terence I
authors.initials: TI
authors.lastname: Moy
authors.forename: Boris
authors.initials: B
authors.lastname: Wilson
authors.forename: Suzanne
authors.initials: S
authors.lastname: Komili
authors.forename: Paul
authors.initials: P
authors.lastname: Tempst
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Roth
authors.forename: Richard A
authors.initials: RA
authors.lastname: Young
authors.forename: Bradley R
authors.initials: BR
authors.lastname: Cairns
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Silver
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002469:Cell Separation; D002843:Chromatin / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020123:Sirolimus / Q000494:pharmacology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015003:Yeasts / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
chemical_list: D000935:Antifungal Agents; D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D016877:Oxidants; C103337:RSC complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); C083324:TOR1 protein, S cerevisiae; D020123:Sirolimus