title: FT-IR microscopic characterization of normal and malignant human colonic tissues.
abstract: Fourier-transform infrared spectroscopy (FT-IR) employs a unique approach to optical diagnosis of tissue pathology based on the characteristic molecular vibrational spectra of the tissue. In this study, we report infrared absorption spectra of formalin fixed, paraffin embedded normal and malignant human colonic tissues from ten different patients. Our method is based on microscopic infrared study (FT-IR-microscopy) of thin tissue specimens in parallel with normal histopathological analysis, which serves as a reference. Our results indicate that the normal colonic tissue has a stronger absorption than the cancerous type over a wide region in all ten cases. The detailed analysis showed that there is a significant decrease in carbohydrate levels, total phosphate and also possibly creatine contents for cancerous tissue in comparison to the controls. Also, RNA/DNA ratio increased in cancerous tissues relative to the normals in all the patients. The results of Linear Discriminant Analysis (LDA) showed that the normal and malignant cells could be identified with about 89% accuracy.
authors.affiliation: Department of Physics, Ben-Gurion University, Beer-Sheva, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Salman
authors.forename: S
authors.initials: S
authors.lastname: Argov
authors.forename: J
authors.initials: J
authors.lastname: Ramesh
authors.forename: J
authors.initials: J
authors.lastname: Goldstein
authors.forename: I
authors.initials: I
authors.lastname: Sinelnikov
authors.forename: H
authors.initials: H
authors.lastname: Guterman
authors.forename: S
authors.initials: S
authors.lastname: Mordechai
mesh_terms: D003106:Colon / Q000033:anatomy & histology* / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D004247:DNA / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D016002:Discriminant Analysis; D006801:Humans; D010710:Phosphates / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D004273:DNA, Neoplasm; D010710:Phosphates; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA