The antibody response to bacteriophage is linked to the lymphopenia gene in congenic BioBreeding rats.
Congenic BioBreeding (BB) rats, homozygous for the autosomal lymphopenia (Lyp) gene (Lyp/Lyp), heterozygous (Lyp/+), or wild-type (+/+), were immunized with the T cell-dependent bacteriophage PhiX174 to determine effects of Lyp on primary and secondary antibody responses. The primary PhiX174 antibody response did not differ between the three different genotypes. In contrast, the secondary immune response, expressed as the peak neutralizing titer, was markedly reduced in Lyp/Lyp (9.9+/-3.2; mean value+/-S.E.M. for seven rats) compared to both Lyp/+ (51+/-12; n=13; P=0.006) and +/+ (100+/-20; n=7; P=0.004) BB rats. We suggest that the secondary antibody response to the T cell-dependent neoantigen PhiX174 is linked in a recessive manner to genetic factor(s) in the Lyp gene region.
Clark
Leila
L
Robert H. Williams Laboratory, Department of Medicine, University of Washington, Seattle, WA 98125, USA.
Greenbaum
Carla
C
Jiang
Jianjie
J
Lernmark
Ake
A
Ochs
Hans
H
D000818:Animals; D020296:Animals, Congenic; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D010584:Bacteriophage phi X 174 / Q000276:immunology*; D004195:Disease Models, Animal; D008231:Lymphopenia / Q000097:blood / Q000276:immunology*; D051381:Rats
D000914:Antibodies, Viral