title: Development of a rat C6 brain tumor model.
abstract: OBJECTIVE
  To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume.
  
  
  METHODS
  C6 cell suspension (10 microl) containing 10 g/L agarose and 1 x 10(6) cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains.
  
  
  RESULTS
  Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%.
  
  
  CONCLUSIONS
  A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.
authors.affiliation: Neurosurgical Institute of PLA, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China. xzhang@fmmu.edu.cn
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wu
authors.forename: Dakuan
authors.initials: D
authors.lastname: Gao
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fei
authors.forename: Yan
authors.initials: Y
authors.lastname: Qu
authors.forename: Junjie
authors.initials: J
authors.lastname: Jing
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology; D004195:Disease Models, Animal; D005910:Glioma / Q000473:pathology; D008279:Magnetic Resonance Imaging; D051381:Rats