Mutant MHC class I molecules define interactions between components of the peptide-loading complex.
Class I histocompatibility molecules, consisting of a heavy chain, beta2-microglobulin and peptide, are assembled in the endoplasmic reticulum (ER) with the assistance of several molecular chaperones and accessory proteins. Peptide binding occurs when assembling class I molecules associate with a loading complex consisting of the transporter associated with antigen processing (TAP) peptide transporter, tapasin, ERp57 and calreticulin (CRT)/calnexin. To assess the physical organization of this complex, we generated a series of mutants in the murine H-2Dd heavy chain and assessed their association with components of the complex. Seven mutations, clustered between amino acids 122 and 136 in the heavy chain alpha2 domain plus one mutation at position 222 in the alpha3 domain, resulted in loss of interaction with tapasin. Association with TAP was always lost simultaneously, supporting the view that tapasin acts as an obligatory bridge between class I molecules and TAP. Compared with previous studies on the HLA-A2 molecule, some differences in points of tapasin interaction were observed. Failure of the H-2Dd mutants to bind tapasin resulted in low cell-surface expression and altered intracellular transport. Most mutants retained a substantial degree of peptide loading, consistent with the view that although tapasin may promote peptide binding to class I, it is not required. A surprising observation was that all mutants lacking tapasin interaction retained normal association with CRT. This contrasts with previous observations on other class I molecules and, combined with differences in tapasin interaction, suggests that the organization of the ER peptide-loading complex can vary depending on the specific class I molecule examined.
Paquet
Marie-Eve
ME
Department of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
Williams
David B
DB
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D037281:Calnexin; D037282:Calreticulin; D002352:Carrier Proteins / Q000378:metabolism; D002450:Cell Communication; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D005803:Genes, Immunoglobulin / Q000235:genetics; D006183:H-2 Antigens / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D064532:Histocompatibility Antigen H-2D; D007136:Immunoglobulins / Q000378:metabolism*; D066298:In Vitro Techniques; D007739:L Cells; D026901:Membrane Transport Proteins / Q000378:metabolism*; D051379:Mice; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D012261:Ribonucleoproteins / Q000378:metabolism
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D017920:Antiporters; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D002352:Carrier Proteins; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D; D007136:Immunoglobulins; D026901:Membrane Transport Proteins; D012261:Ribonucleoproteins; C073119:TAP1 protein, human; C486171:Tap1 protein, mouse; C107762:tapasin; D037281:Calnexin; C043912:peptide permease