[Studies on urinary metabolites of HH07A, a derivative of hainanensine, in rats].
The metabolism of HH07A in the rat has been investigated by GC-MS. After oral administration of HH07A, the rat urine was passed through a macroporous XAD-2 resin column and eluted with methanol. The methanol extract was hydrolyzed with glucuronidase, extracted with dichloromethane and concentrated for TMS derivatization with GC-MS. The mass spectra of HH07A and its four metabolites as well as their derivatives were presented. The structures of the metabolites were proposed and the in vivo metabolic pathway of HH07A was given in Figure 6.
Zhang
W
W
Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Zhou
T
T
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000138:chemical synthesis / Q000302:isolation & purification*; D032483:Cephalotaxus / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006248:Harringtonines / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism* / Q000652:urine; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000972:Antineoplastic Agents, Phytogenic; C095422:HH07A; D006248:Harringtonines; C030424:hainanensine