[Malignant transformation of HPV-18 immortalized human bronchial epithelial cells induced by tobacco-specific nitrosamine NNK].
The malignant transformation of HPV-18 immortalized human bronchial epithelial cell(BEP2D) induced by tobacco-specific nitrosamine NNK and its biological characteristics were investigated. BEP2D cells treated with NNK at the concentration of 500 micrograms/ml for 24 hours could be subcultured continuously in vitro. The 15th passage cells in soft agar (0.038%) increased 11 fold compared with that of control cells (0.0033%). The 25th passage cells could grow into tumor in nude mice. The tumor was a squamous cell carcinoma in morphology confirmed by histopathological examination. The results indicated that the malignant transformation induced by NNK was a sequential process. This culture system provides a potential tool for the study of cell and molecular mechanism in the multistage carcinogenesis of human bronchial epithelial cells.
Sun
H
H
Institute of Transfusion, Beijing 100850, China.
Gong
Y
Y
Wang
Q
Q
Chen
J
J
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D001980:Bronchi / Q000473:pathology*; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins; D002273:Carcinogens / Q000633:toxicity; D002471:Cell Transformation, Neoplastic; D004847:Epithelial Cells / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009602:Nitrosamines / Q000633:toxicity*; D009856:Oncogene Proteins, Viral / Q000378:metabolism*
D000956:Antigens, Viral; D036022:Capsid Proteins; D002273:Carcinogens; C099170:L2 protein, Human papillomavirus type 18; D009602:Nitrosamines; D009856:Oncogene Proteins, Viral