title: Association of a regulatory gene, slyA with a mouse virulence of Salmonella serovar Choleraesuis.
abstract: The influence of slyA gene, originally found in Salmonella serovar Typhimurium as a regulatory gene for the expression of virulence genes, on a mouse virulence of S. serovar Choleraesuis was investigated by using an slyA-defective mutant. The defective mutant was constructed by the insertion of a kanamycin-resistance gene (aph) into the cloned slyA gene, and the homologous recombination with the intact slyA gene on the chromosome. The mutant strain showed the LD50 value for BALB/c mouse approximately 10(5) higher than that of the parent strain. The increase of the LD50 value was the same order as that shown by the mutation of the slyA gene of S. serovar Typhimurium, although LD50 of the wild-type strain of S. serovar Choleraesuis was 40-fold higher than that of S. serovar Typhimurium. The time course of infection observed in the mice organs also proved the clear difference of the virulence between the parent and the mutant strains. These results suggested that the slyA gene product functions as a virulence-associated regulator also in S. serovar Choleraesuis.
authors.affiliation: Department of Microbiology, School of Pharmaceutical Science, Kitasato University, Tokyo, Japan. kaneko-a@kitasato.or.jp
authors.forename: Aki
authors.initials: A
authors.lastname: Kaneko
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mita
authors.forename: Kachiko
authors.initials: K
authors.lastname: Sekiya
authors.forename: Hidenori
authors.initials: H
authors.lastname: Matsui
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Kawahara
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Danbara
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics*; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011995:Recombination, Genetic; D012475:Salmonella / Q000235:genetics / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000382:microbiology*; D014157:Transcription Factors; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D014157:Transcription Factors; C056785:salmolysin