Congenic mapping of a blood pressure QTL on chromosome 16 of Dahl rats.
A Chromosome (Chr) 16 segment of the Dahl salt-sensitive (S) rat was shown by linkage to contain a blood pressure (BP) quantitative trait locus (QTL). To verify and further narrow down the region harboring the QTL, we made two congenic strains by replacing two segments of the S rats with the homologous segments of the Lewis (LEW) rats. The construction of these congenic strains was facilitated by a genome-wide marker screening. The two congenic strains contained a segment in common, and BPs of both were significantly lower than that of the S strain. Consequently, a BP QTL could be localized to the overlapping region of about 49.4 centiRay (cR) including the telomere on a radiation hybrid map. Heart weights, left and right ventricular weights, kidney weights, and aortic weights over length were all significantly decreased in the congenic strains compared with the S strain. Thus, there appeared to exist an association between the effects of the QTL on BP and on cardiac, renal, and vascular hypertrophy.
Moujahidine
Myriam
M
Research Centre, Centre Hospitalier de l'Universite de Montreal (CHUM), 7-132 Pavillon Jeanne Mance, 3840, rue St. Urbain, Montreal, Quebec, H2W 1T8, Canada.
Dutil
Julie
J
Hamet
Pavel
P
Deng
Alan Y
AY
D000818:Animals; D020296:Animals, Congenic; D001794:Blood Pressure / Q000235:genetics*; D002874:Chromosome Mapping; D019655:Quantitative Trait, Heritable; D051381:Rats; D020303:Rats, Inbred Dahl