A component with high molecular weight is prepared from commercial bovine insulin by gel filtration on Sephadex G 50. The substance has little biological and immunological activity in comparison to insulin. The activity is not changed by incubation with trypsin. It associates in 1 M CH(3)COOH and possesses a molecular weight of 28,500 as shown by ultracentrifugation. The ability of the component to precipitate with bovine insulin antibodies demonstrates that it contains immunologically active insulin-like proteins and is not a homogenous substance.
Zühlke
H
H
Zentralinstitut für Diabetes "Gerhardt Katsch", Bereich experimentelle Diabetesforschung, Karlsburg bei, Greifswald, DDR
Kohnert
K -D.
K
Ziegler
M
M
Knospe
S
S
Behlke
J
J
Fraktionierung handelsüblichen insulins. Gewinnung und eigenschaften einer hochmolekularen, insulin-ähnlichen komponente.