Borrelia burgdorferi are susceptible to killing by a variety of human polymorphonuclear leukocyte components.
The killing of Borrelia burgdorferi by intact human polymorphonuclear leukocytes (PMNL) and by individual PMNL components was compared. Intact PMNL killed B. burgdorferi 6.5-fold more efficiently and 5-fold more completely when spirochetes were opsonized with specific antibodies. U-cytoplasts, which have activatable oxidase, killed opsonized B. burgdorferi with an efficiency similar to that of intact PMNL in killing unopsonized B. burgdorferi. Although B. burgdorferi were susceptible to H(2)O(2) and nitric oxide, PMNL lysates killed B. burgdorferi nearly as well as intact PMNL killed opsonized B. burgdorferi, suggesting a critical role for granule contents. B. burgdorferi were killed by the PMNL antimicrobial components elastase, LL-37, bactericidal/permeability-increasing protein, and human neutrophil peptide-1. B. burgdorferi had limited susceptibility to killing by lysozyme and were not killed by azurocidin, proteinase 3, or lactoferrin. The efficient killing of B. burgdorferi by a variety of PMNL mechanisms highlights the paradoxical persistence of spirochetes in vivo.
Lusitani
Denise
D
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8031, USA.
Malawista
Stephen E
SE
Montgomery
Ruth R
RR
D023181:Antimicrobial Cationic Peptides / Q000502:physiology; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000502:physiology; D025065:Borrelia burgdorferi / Q000276:immunology*; D002352:Carrier Proteins / Q000502:physiology; D054804:Cathelicidins; D006801:Humans; D007781:Lactoferrin / Q000502:physiology; D019272:Leukocyte Elastase / Q000502:physiology; D008565:Membrane Proteins; D009113:Muramidase / Q000502:physiology; D053146:Myeloblastin; D009504:Neutrophils / Q000276:immunology*; D009569:Nitric Oxide / Q000502:physiology; D012697:Serine Endopeptidases / Q000502:physiology; D023084:alpha-Defensins / Q000502:physiology
C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D054804:Cathelicidins; D008565:Membrane Proteins; D023084:alpha-Defensins; C021623:bactericidal permeability increasing protein; C406413:human neutrophil peptide 1; D009569:Nitric Oxide; D009113:Muramidase; D007781:Lactoferrin; D012697:Serine Endopeptidases; D019272:Leukocyte Elastase; D053146:Myeloblastin