[Inhibition of telomerase activity and induction of apoptosis in lung cancer cell by human telomerase reverse transcriptase gene antisense oligodeoxynucleotide].
OBJECTIVE
To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (ASODN) on telomerase activity and apoptosis in lung cancer cell A549 line.


METHODS
Cultured cells were divided into three groups: ASODN (5'-GCGCGGCAGCGCGGGGGTGGCCG-3'), sense oligodeoxynucleotide (5'-CGGCCACCCCCGCGATGCCGCGC-3', SODN) and control. The concentration of oligodeoxynucleotide and lipsome was 10 micromol/L and 16 mg/L respectively. The changes of telomerase activity, expression of hTERT mRNA and protein and apoptosis were observed in different groups.


RESULTS
hTERT mRNA and protein levels, as well as telomerase activity began to be down-regulated or inhibited when A549 cells were treated with ASODN for 72 h, but apoptosis was induced until cells were treated for 21 days with ASODN (rate of apoptosis was 0.88%, 2.77% and 12.00% for the control, SODN and ASODN group respectively).


CONCLUSIONS
It is suggested that hTERT ASODN might specially inhibit telomerase activity at translation level in lung cancer cells and it is further proved that the hTERT gene have significant correlation with telomerase activity. Further evidence is needed to prove whether hTERT ASODN might be a potential tool for the treatment of lung cancer.
Wang
Xiaoyang
X
Research Laboratory of Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhang
Zhenxiang
Z
Xu
Yongjian
Y
Chen
Shixin
S
Xiong
Weining
W
D017209:Apoptosis; D004268:DNA-Binding Proteins; D006801:Humans; D008175:Lung Neoplasms; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D019098:Telomerase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D019098:Telomerase