Chiral separation of gemifloxacin in sodium-containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis.
Chiral crown ether, (+)-(18-crown-6)-tetracarboxylic acid (18C6H(4)), is an effective chiral selector for resolving enantiomeric primary amines owing to the difference in affinities between 18C6H(4) and each of the amine enantiomers. In addition to the destacking effect of sodium ion in the sample solution, the strong affinity of sodium ion to the polyether ring of crown ether is unfavorable to chiral capillary electrophoresis using 18C6H(4) as a chiral selector. In this report, the chiral separation of gemifloxacin dissolved in a saline sample matrix using 18C6H(4) was investigated. Adding a chelating agent, ethylenediaminetetraacetic acid (EDTA), to the run buffer greatly improved the separation efficiencies and peak shapes. The successful chiral separation of gemifloxacin in a urinary solution was demonstrated for both capillary and microchip electrophoresis.
Cho
Seung Il
SI
School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Korea.
Lee
Kook-Nyung
KN
Kim
Yong-Kweon
YK
Jang
Jaeho
J
Chung
Doo Soo
DS
D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D043844:Crown Ethers; D004492:Edetic Acid; D019075:Electrophoresis, Capillary / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D006801:Humans; D015394:Molecular Structure; D009287:Naphthyridines / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D012964:Sodium
D000890:Anti-Infective Agents; D043844:Crown Ethers; D004988:Ethers, Cyclic; D024841:Fluoroquinolones; D009287:Naphthyridines; C090732:18-crown-6 2,3,11,12-tetracarboxylic acid; D004492:Edetic Acid; D012964:Sodium; D000077735:Gemifloxacin