Thirteen single-nucleotide polymorphisms (SNPs) in the alcohol dehydrogenase 4 (ADH4) gene locus.
The human alcohol dehydrogenase 4 (ADH4) gene encodes the class II ADH4 pi subunit, which contributes to the metabolization of a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Here we report the results of systematic screening for single-nucleotide polymorphisms (SNPs) in the ADH4 gene by means of direct sequencing combined with a polymerase chain reaction method. A total of 16 genetic variations including 13 SNPs were found; 4 in the 5' flanking region, 4 in the 5' untranslated region, and 8 within introns. No variation was found in coding, 3' untranslated, or 3' flanking regions. Eight of the 13 SNPs were not reported in the NCBI dbSNP database or any previous publications. Our SNP map presented here should provide tools to evaluate the role of ADH4 in complex genetic diseases and a variety of pharmacogenetic effects.
Iida
Aritoshi
A
RIKEN SNP Research Center, Institute of Medical Science, The University of Tokyo, Japan. ariiida@ims.u-tokyo.ac.jp
Saito
Susumu
S
Sekine
Akihiro
A
Kondo
Kimie
K
Mishima
Chihiro
C
Kitamura
Yuri
Y
Harigae
Satoko
S
Osawa
Saori
S
Nakamura
Yusuke
Y
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D002874:Chromosome Mapping; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D000426:Alcohol Dehydrogenase; C105552:alcohol dehydrogenase IV