title: Hepatitis C virus RNA detection by in situ RT-PCR in formalin-fixed paraffin-embedded liver tissue. Comparison with serum and tissue results.
abstract: The purpose of this study was to localize HCV RNA in formalin-fixed paraffin-embedded liver biopsies of 15 patients with chronic hepatitis C using in situ RT-PCR method. The results were compared to serum and tissue extract analysis of HCV RNA. HCV RNA was detected in 80% of the sera tested, in 40% of the corresponding hepatic tissue extract and in 60% of the tissue sections tested by in situ RT-PCR. Compared to the serum positive cases, 67% of the cases were positive with in situ RT-PCR and 41% were positive with tissue extract detection. 50% of the cases in situ RT-PCR positive were also positive with tissue extract detection. These results underlined the complementarity of the different methods of viral detection for the precise diagnosis of hepatitis C.
authors.affiliation: Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, Marseille, France. pbiagini-ets-ap@mail.guilliver.fr
authors.forename: P
authors.initials: P
authors.lastname: Biagini
authors.forename: L
authors.initials: L
authors.lastname: Benkoel
authors.forename: F
authors.initials: F
authors.lastname: Dodero
authors.forename: X
authors.initials: X
authors.lastname: de Lamballerie
authors.forename: V
authors.initials: V
authors.lastname: Chamlian
authors.forename: H
authors.initials: H
authors.lastname: Nouhou
authors.forename: V
authors.initials: V
authors.lastname: Gerolami
authors.forename: P
authors.initials: P
authors.lastname: de Micco
authors.forename: A
authors.initials: A
authors.lastname: Chamlian
mesh_terms: D005557:Formaldehyde; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D008099:Liver / Q000821:virology; D010232:Paraffin; D012367:RNA, Viral / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016610:Tissue Embedding; D016707:Tissue Fixation; D014773:Virology / Q000379:methods
chemical_list: D012367:RNA, Viral; D005557:Formaldehyde; D010232:Paraffin