Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism.
An 8 year old girl with cystic fibrosis presented with a pulmonary exacerbation from which Burkholderia cepacia was cultured. Subsequent polymerase chain reaction restriction fragment length polymorphism analysis of the recA gene suggested the presence of B cepacia Genomovar V (Burkholderia vietnamiensis); however, on subsequent sequence typing, this isolate was confirmed as B cepacia Genomovar IIIb. This report outlines the potential difficulties in the correct characterisation of the various genomovars within the B cepacia complex of organisms, which has particularly important implications for patient segregation and infection control.
Moore
J E
JE
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, UK. jemoore@niphl.dnet.co.uk
Millar
B C
BC
Xu
J
J
Crowe
M
M
Redmond
A O B
AO
Elborn
J S
JS
D015373:Bacterial Typing Techniques / Q000379:methods; D001483:Base Sequence; D019121:Burkholderia Infections / Q000150:complications / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications*; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009894:Opportunistic Infections / Q000150:complications / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length