[Effects of murine bone marrow endothelial cell conditioned medium in combination with FL and TPO on the growth of HPP-CFC and CFU-GM].
In the present study, the effects of murine bone marrow endothelial cell conditioned medium (ECM) combined with flt3 ligand (FL) or/and thrombopoietin (TPO) on the proliferation of HPP-CFC and CFU-GM were investigated. Both ECM and the concentrated retentate of ECM (MW>10 kD) promoted the growth of CFU-GM and HPP-CFC, and this promoting effect was further enhanced by addition of FL or TPO. Using reverse transcriptase-polymerase chain reaction (RT-PCR) technique, the expression of FL and TPO mRNA was not found in murine bone marrow endothelial cells.
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Research Laboratory of Blood Physiology,Hunan Medical University, Changsha 410078.
Wang
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D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D042783:Endothelial Cells / Q000166:cytology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006410:Hematopoiesis / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008565:Membrane Proteins / Q000494:pharmacology*; D051379:Mice; D012333:RNA, Messenger; D013926:Thrombopoietin / Q000494:pharmacology*
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D008565:Membrane Proteins; D012333:RNA, Messenger; C084881:flt3 ligand protein; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013926:Thrombopoietin