title: Bacteria and pathogenesis of human salivary calculus.
abstract: The exact cause of salivary calculus formation is unknown and the aim of this study was to ascertain whether bacteria play a role. Sialoliths from nine patients with chronic obstructive sialadenitis of the submandibular gland were analysed. Bacterial gene fragments were amplified from DNA extracted from salivary calculi by means of polymerase chain reaction using a universal bacterial primer pair. Comparative 16S ribosomal RNA sequence analysis was used for identification. We detected and identified oral bacteria (predominantly Streptococcus species) in all samples. The present results suggest a potential role for bacteria in the etiopathogenesis of sialolithiasis.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Philipps University, Marburg, Germany. teymoort@mailer.uni-marburg.de
authors.forename: Afshin
authors.initials: A
authors.lastname: Teymoortash
authors.forename: Ana C
authors.initials: AC
authors.lastname: Wollstein
authors.forename: Burkard M
authors.initials: BM
authors.lastname: Lippert
authors.forename: Roswitha
authors.initials: R
authors.lastname: Peldszus
authors.forename: Jochen A
authors.initials: JA
authors.lastname: Werner
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015494:Salivary Gland Calculi / Q000382:microbiology*; D017423:Sequence Analysis, RNA; D013291:Streptococcus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D013364:Submandibular Gland Diseases / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S