Lack of isoprene overproduction during peritoneal dialysis.
OBJECTIVE
Isoprene is the constitutive unit of isoprenoid lipids and sterols. However, it is also a potential toxic and carcinogenic agent. Recent findings of a marked and prolonged isoprene overproduction induced by hemodialysis sessions raises the question of isoprene behavior in patients on peritoneal dialysis.


METHODS
A study with repeated measures per patient and healthy control.


METHODS
Nephrology and Dialysis Unit and Perugia University Medical School.


METHODS
Sixteen consecutive patients on regular continuous ambulatory peritoneal dialysis (CAPD) were evaluated. Endogenous isoprene was analyzed using gas chromatographic assay of breath isoprene, collected at set times before and after dialysis fluid exchange.


RESULTS
No significant variations were found in breath isoprene concentrations in the different samples from each patient, and levels were almost stable within the normal range of healthy controls.


CONCLUSIONS
These results show that CAPD, unlike hemodialysis, has little or no effect on isoprene and isoprenoid-related lipid turnover. This lack of increased endogenous isoprene synthesis, in addition to being a distinctive metabolic feature of CAPD, could have important pathophysiological and clinical implications.
Capodicasa
Enrico
E
Department of Clinical and Experimental Medicine, Perugia University Medical School, Italy. encap@unipg.it
Trovarelli
Gianfranco
G
Brunori
Federica
F
Vecchi
Luigi
L
Carobi
Carmen
C
De
MedioGiannaE
M
Pelli
Maria A
MA
Buoncristiani
Umberto
U
D000328:Adult; D000368:Aged; D001944:Breath Tests; D002070:Butadienes / Q000032:analysis / Q000378:metabolism*; D002940:Circadian Rhythm; D005260:Female; D045782:Hemiterpenes; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010420:Pentanes; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013997:Time Factors
D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene