The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.
The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unknown mechanism that does not involve cohesin cleavage. We show that Polo-like kinase is required for the cleavage-independent dissociation of cohesin from chromosomes in Xenopus. Cohesin phosphorylation depends on Polo-like kinase and reduces the ability of cohesin to bind to chromatin. These results suggest that Polo-like kinase regulates the dissociation of cohesin from chromosomes early in mitosis.
Sumara
Izabela
I
Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Vorlaufer
Elisabeth
E
Stukenberg
P Todd
PT
Kelm
Olaf
O
Redemann
Norbert
N
Nigg
Erich A
EA
Peters
Jan-Michael
JM
D000818:Animals; D064127:Aurora Kinases; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002458:Cell Fractionation; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D014020:Tissue Extracts / Q000737:chemistry / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis; D000097722:Cohesins; D000097869:Polo-Like Kinase 1
D064127:Aurora Kinases; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D014020:Tissue Extracts; D029867:Xenopus Proteins; D000097722:Cohesins; D000097869:Polo-Like Kinase 1; C112134:AURKA protein, Xenopus