DNA sequence analysis of the triose phosphate isomerase gene from isolates of Giardia lamblia.
OBJECTIVE
To confirm the genetic relation between Giardia lamblia (G. lamblia) isolates from different geographic regions of China and other countries.


METHODS
Genomic DNA were extracted from the trophozoites or cysts of Giardia lamblia. The triose phosphate isomerase (tim) gene was amplified using polymerase chain reaction (PCR) technique. PCR products were digested with endonuclease and sequenced. The data of sequencing were analyzed with the DNAstar software and compared with that of the isolates acquired from GenBank.


RESULTS
Of nine isolates of Giardia lamblia from China (C1, C2, CH2 and CH3), Cambodia (CAM), Australia (A1 and A2) and America (BP and CDC), respectively, 3 (A1, A2 and CAM) fit into Group 1 (WB), 2 (CH2 and CH3)) into Group 2, and 4 (C1, C2, BP and CDC) into Group 3 (GS). The results confirmed the genetic relatedness of G. lamblia isolates from all over the world.


CONCLUSIONS
Genotyping isolates of G. Lamblia provides important information for establishing the phylogenetic relationship or for the epidemiological evaluation of the spreading of this organism.
Lu
Siqi
S
Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China. Lusiqi@cpums.edu.cn
Wen
Jianfan
J
Li
Jihong
J
Wang
Fengyun
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry*; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014305:Triose-Phosphate Isomerase / Q000737:chemistry / Q000235:genetics*
D016054:DNA, Protozoan; D014305:Triose-Phosphate Isomerase