[Implantation of skeletal muscle stem cells in inhibition of fibroatrophy of ischemic myocardium: an experimental study].
OBJECTIVE
To investigate the effect of stem cells derived from autogenous skeletal muscle, namely satellite cells, and implanted into ischemic myocardium on inhibition of myocardium fibroatrophy.


METHODS
The left anterodecendant arteries (LAD) of 12 adult dogs were ligated so as to establish animal model of acute myocardiac infarction. Satellite cells isolated from the greatest gluteal muscle of dogs were labeled with 4' 6-dimidino-2-phenylindone (DAPI), and then infused into the ventricular myocardium of the isogenic dogs through LAD. Specimens of ischemic myocardium were taken 2, 4, and 8 weeks after myoblast implantation. Histologic sections are examined under common light microscope and fluorescent microscope. Twelve dogs were used as controls.


RESULTS
In the ischemic myocardium where satellite cells had been implanted, fibroaastrophy was efeectively inhibited. The implanted satellite cells had differentiated into fully developed striated muscle cells. Vitreous degeneration and disorder of basic structure could be observed in the ischemic myocardium in the control group.


CONCLUSIONS
The satellite cells from autologous skeletal muscle differentiate into cardiac muscle cell-like cells in the ischemic area and inhibit fibroatrophy of the ischemic myocardium after implantation, thus bringing a hope of a new cure for myocardial damage.
Wei
H
H
Department of Chest Surgerey, First Hospital Affiliated to Henan Medical University, Zhengzhou 450052, China.
Zhu
H
H
Zhao
G
G
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D004195:Disease Models, Animal; D004285:Dogs; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods / Q000639:trends; D018482:Muscle, Skeletal / Q000166:cytology; D009203:Myocardial Infarction / Q000473:pathology* / Q000628:therapy; D017202:Myocardial Ischemia / Q000473:pathology* / Q000628:therapy; D027161:Satellite Cells, Perineuronal / Q000637:transplantation*; D014182:Transplantation, Autologous / Q000379:methods