Detection of LOH of the RB1 gene in bladder cancers by PCR-RFLP.
OBJECTIVE
Retinoblastoma (RB1) gene involves in retinoblastoma, osteosarcoma, bladder, prostate, lung, breast carcinomas, and soft tissue sarcomas. Loss of heterozygosity (LOH) is the most common mutation of the gene.


METHODS
Xba I polymorphism in intron 17 of the gene was used to detect LOH in 20 bladder cancer patients. A cystitis and an osteosarcoma were used as control. LOH was investigated in three different kinds of samples (blood, paraffin-embedded tissue and fresh tissue) belonging to the same patients, and 20 blood samples, 20 paraffin-embedded tissue samples and 16 fresh tissue samples were obtained from 20 cancer patients.


RESULTS
None of the 20 blood samples showed LOH. Eleven out of 20 paraffin-embedded bladder tissues were amplified, 3 of them homozygous and all 8 informative paraffin-embedded tissues showed LOH. Five out of 16 fresh tumor tissues obtained were amplified, in 1 the fresh tissue was normal, 1 fresh tissue showed LOH and 3 were not digested by Xba I.


CONCLUSIONS
The results of the study have suggested that detection of LOH of the RB1 gene by PCR-RFLP can be a good adjunctive test for evaluation of the bladder cancer.
Açikbas
I
I
Department of Medical Biology, Faculty of Medicine, Pamukkale University, Denizli, Turkey. iacikbas@pamukkale.edu.tr
Keser
I
I
Kiliç
S
S
Bağci
H
H
Karpuzoğlu
G
G
Lüleci
G
G
D002295:Carcinoma, Transitional Cell / Q000235:genetics; D016161:Genes, Retinoblastoma; D006801:Humans; D019656:Loss of Heterozygosity; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012175:Retinoblastoma / Q000235:genetics; D001749:Urinary Bladder Neoplasms / Q000235:genetics*