[Tissue engineering study on chitosan-gelatin/hydroxyapatite composite scaffolds--osteoblasts culture].
OBJECTIVE
To investigate the behavior of rat calvarial osteoblasts cultured on chitosan-gelatin/hydroxyapatite (CS-Gel/HA) composite scaffolds.


METHODS
The rat calvarial osteoblasts (the 3rd passage) were seeded at a density of 1.01 x 10(6) cells/ml onto the CS-Gel/HA composite scaffolds having porosity 85.20%, 90.40% and 95.80%. Cell number was counted after cultured for 3 days, 1 week, 2 weeks and 3 weeks. Cell proliferation, bone-like tissue formation, and mineralization were separately detected by HE, von Kossa histological staining techniques.


RESULTS
The CS-Gel/HA composite scaffolds supported the attachment of seeded rat calvarial osteoblasts. Cells proliferated faster in scaffold with higher porosity 90.40% and 95.80% than scaffold with lower porosity 85.20%. The osteoblasts/scaffold constructs were feasible for mineral deposition, and bone-like tissue formation in 3 weeks.


CONCLUSIONS
This study suggests the feasibility of using CS-Gel/HA composite scaffolds for bone tissue engineering.
Zhao
Feng
F
Research Institute of Polymeric Materials, Tianjin University, Tianjin, P. R. China 300072.
Yin
Yu-Ji
YJ
Yao
Kang-De
KD
Guo
Gang
G
Wang
Bo-Li
BL
Zhang
Jing-Yu
JY
Zhang
Ming-fang
MF
D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives*; D005109:Extracellular Matrix; D005780:Gelatin; D006882:Hydroxyapatites; D010006:Osteoblasts / Q000502:physiology*; D051381:Rats; D023822:Tissue Engineering
D001672:Biocompatible Materials; D006882:Hydroxyapatites; D002686:Chitin; D005780:Gelatin