title: An improved clean-up strategy for simultaneous analysis of polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF), and polychlorinated biphenyls (PCB) in fatty food samples.
abstract: The study and extension of a simple automated clean-up method for polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) to a broad range of polychlorinated biphenyls (PCB) is described. The isolation of seven PCDD, ten PCDF, and three coplanar PCB (cPCB) is extended to eight monoortho substituted PCB and seven so-called "marker PCB" (Aroclor 1260) for fatty food samples. This enables quantification of 35 compounds - including all congeners with a WHO toxic equivalent factor (TEF)--in a single extraction and single purification step. The chromatographic behaviour of mono-ortho PCB and marker PCB on a variety of adsorbents, including basic alumina, has been studied. Partitioning of analytes through multi-column sequences is described and correlated with their structural and electronic properties, by use of molecular modelling calculations. The fractionation process available with the Power-Prep automated clean-up system enables rapid independent analysis of the different groups of compounds. Gas chromatography with high resolution mass spectrometry (GC-HRMS) is used for the PCDD/F and cPCB fraction and quadrupole ion-storage tandem in time mass spectrometry (GC-QISTMS) for analysis of the remaining PCB. A comparison study was performed on quality-control samples and real fatty food samples to evaluate the robustness of the new strategy compared with a reference method. On the basis of this simultaneous clean-up, a rapid simplified strategy for PCDD/F and selected PCB analysis determination is proposed for fatty food samples.
authors.affiliation: Mass Spectrometry Laboratory, University of Liege, Belgium.
authors.forename: Catherine
authors.initials: C
authors.lastname: Pirard
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Focant
authors.forename: Pauw Edwin
authors.initials: PE
authors.lastname: De
mesh_terms: D000818:Animals; D001331:Automation; D001572:Benzofurans / Q000032:analysis; D005591:Chemical Fractionation; D000072338:Dibenzofurans, Polychlorinated; D004785:Environmental Pollutants / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000592:standards; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D008460:Meat / Q000032:analysis; D008958:Models, Molecular; D011078:Polychlorinated Biphenyls / Q000032:analysis; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D011786:Quality Control; D012015:Reference Standards
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D000072317:Polychlorinated Dibenzodioxins; D011078:Polychlorinated Biphenyls