[Significance of detection of HBV-DNA and CMV-DNA by polymerase chain reaction in screening mothers' milk].
Hepatitis B virus(HBV) DNA and cytomegalovirus DNA in the colostrum of lying-in women were detected by polymerase chain reaction for the safety of mothers' milk in the milk bank. The results showed that: 1. HBV-DNA was not found in the milk of women with negative serological markers of the virus, while in the milk of women with positive marker of HBsAg, HBeAg and HBcAb, the positive rate of HBV-DNA was 81.25%, in the milk of mothers with positive marker of HBsAg, HBcAb and HBeAb, the positive rate was 45.24%, in the milk of women only with HBcAb and HBeAb positive, the rate was 23.08%. 2. The specific amplified products of CMV DNA in the milk were obtained in 42.80%-70.00% women whose CMV IgM antibody was positive. These findings suggest that the excretion of viruses would increase in mothers with active virus replication, and the viruses would be transmitted to newborns by milk. The authors think that only the milk from the mothers with both negative serological markers and negative PCR of the viruses can be collected and stored in the milk bank. PCR is a specific and sensitive technique which can be used to monitor the safety of mothers' milk. In this paper, the authors have presented some suggestions about how to give breast-feeding and protect newborns whose mothers are various status of virus secretion in the milk.
Yang
D
D
Department of Nursing, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Li
Y
Y
Song
J
J
D000328:Adult; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000517:prevention & control / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006509:Hepatitis B / Q000517:prevention & control / Q000821:virology*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008895:Milk, Human / Q000821:virology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy
D004279:DNA, Viral