[Effect of shenkang injection on transforming growth factor-beta messenger ribonucleic acid of LLC-PK1 renal tubular epithelial cells].
OBJECTIVE
To study the effect of Shenkang injection (SKI) on the transforming growth factor (TGF)-beta mRNA of LLC-PK1 renal tubular epithelial cells.


METHODS
Using reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern Blot to observe the effect of SKI on the TGF-beta mRNA of LLC-PK1 renal tubular epithelial cells, with control group treated with the injection of Rheum officinale extraction.


RESULTS
SKI showed the inhibition of TGF-beta mRNA expression of renal tubular epithelial cells, which is dose-dependent and was more obvious than that of the Rheum extractive injection with equal dose of drug.


CONCLUSIONS
The mechanism of delaying chronic renal failure with SKI is achieved by down regulating TGF-beta mRNA expression of renal tubular cells.
Zhao
Z
Z
Beijing University of Traditional Chinese Medicine, Beijing 100029.
Li
H
H
Zhang
X
X
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology; D015870:Gene Expression; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000187:drug effects; D018374:LLC-PK1 Cells; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta