Prominent beta-5 gene expression in the cardiovascular system and in the cartilaginous primordiae of the skeleton during mouse development.
The alpha v beta (alpha(v)beta5) heterodimer has been implicated in many biological functions, including angiogenesis. We report the beta5 gene expression pattern in embryonic and foetal mouse tissues as determined by Northern blotting and in situ hybridization. During the earliest stages, beta5 mRNA is widespread in the mesoderm. During later developmental stages, it remains mostly confined to tissues of mesodermal origin, although probable inductive effects trigger shifts of beta5 gene expression from some mesenchymatous to epithelial structures. This was observed in the teeth, skin, kidneys, and gut. Of physiological importance is the beta5 labeling in the developing cardiovascular and respiratory systems and cartilages. Furthermore, early beta5 gene expression was observed within the intra- and extraembryonic sites of hematopoiesis. This suggests a major role for beta5 in the hematopoietic and angiogenic stem cells and thus in the development of the vascular system. Later, the beta5 gene was expressed in endothelial cells of the vessels developing both by angiogenesis and vasculogenesis in the lung, heart, and kidneys. Moreover, the beta5 hybridization signal was detected in developing cartilages but not in ossified or ossifying bones. beta5-Integrin is a key integrin involved in angiogenesis, vasculogenesis, hematopoiesis, and bone formation.
Le Gat
L
L
Centre de Recherches Thérapeutique en Ophthalmologie Equipe d'accueil du Ministère de la Recherche et de l'Enseignement Supérieur, Université René Descartes, Paris V, Faculté de Médecine, Necker, France.
Bonnel
S
S
Gogat
K
K
Brizard
M
M
Van Den Berghe
L
L
Kobetz
A
A
Gadin
S
S
Dureau
P
P
Dufier
J L
JL
Abitbol
M
M
Menasche
M
M
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001842:Bone and Bones / Q000196:embryology*; D002319:Cardiovascular System / Q000196:embryology* / Q000378:metabolism; D002356:Cartilage / Q000196:embryology* / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000254:growth & development / Q000648:ultrastructure; D051379:Mice / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012137:Respiratory System / Q000378:metabolism
D039641:Integrin beta Chains; C084344:integrin beta5