[Study on endothelial cell injury in women with pregnancy induced hypertension].
OBJECTIVE
To investigate the mechanisms of endothelial cell injury in women with pregnancy induced hypertension (PIH).


METHODS
Human umbilical endothelial cells were cultured in the serum from 10 women with severe PIH and 10 normal pregnant women, and co-cultured with neutrophils or not for 48 hrs. The morphology and cell cycles were studied.


RESULTS
Without neutrophils, no apoptotic cells were observed, while neutrophils were added, apoptotic cells were obviously detected. The percentage of apoptotic cells in PIH was more than that in non-PIH. The percentage of cells in G2/M phase decreased in all the groups, but there were no significant differences among them.


CONCLUSIONS
Endothelial cells were injured indirectly in PIH patients. Activated neutrophils induced apoptosis might be one of the answers.
Zhao
W
W
Maternal and Children Health Center, First Hospital, Peking University, Beijing 100034, China.
Chen
L
L
Wang
L
L
D000328:Adult; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000032:analysis; D017209:Apoptosis; D002453:Cell Cycle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D006973:Hypertension / Q000097:blood*; D015999:Multivariate Analysis; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood*
D019268:Antibodies, Antineutrophil Cytoplasmic