Association study between a novel single nucleotide polymorphism of the promoter region of the prostacyclin synthase gene and essential hypertension.
The purpose of this study was to investigate whether an association exists between the promoter region of the prostacyclin synthase gene and essential hypertension (EH). Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, we discovered a novel single nucleotide polymorphism (SNP), T-192G, in the 5'-flanking region. We performed an association study using the SNP in 200 patients and 200 controls. The allele frequency distribution in the two groups was not significantly different. Thus, this SNP in the PGIS gene is not associated with EH.
Nakayama
Tomohiro
T
Division of Receptor Biology, Advanced Medical Research Center, Tokyo, Japan. tnakayam@med.nihon-u.ac.jp
Soma
Masayoshi
M
Rahmutula
Dolkun
D
Tobe
Hideko
H
Sato
Mikano
M
Uwabo
Jiro
J
Aoi
Noriko
N
Kosuge
Kotoko
K
Kunimoto
Masako
M
Kanmatsuse
Katsuo
K
Kokubun
Shinichiro
S
D000328:Adult; D000483:Alleles; D001483:Base Sequence / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012016:Reference Values; D020080:Tandem Repeat Sequences
D003577:Cytochrome P-450 Enzyme System; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase