title: In vitro activity of sagamicin against ocular bacterial isolates.
abstract: The in vitro antibacterial activity of sagamicin, gentamicin, tobramycin and norfloxacin was evaluated against 180 recent clinical isolates obtained from patients with ocular infections. Good activity was demonstrated for the 3 aminoglycosides against methicillin-sensitive Staphylococcus aureus and Staphylococcus epidermidis. All 4 compounds showed a lower activity against methicillin-resistant staphylococci. Sagamicin was highly effective against enterobacteriaceae with a MIC(90) of 2 mg/l and presented good antipseudomonal activity similar to that of gentamicin. Intraocular lenses impregnated with a sagamicin solution showed a good antistaphylococcal activity immediately after preparation. In our strain collection, the ability to produce slime was more frequent among methicillin-resistant S. epidermidis strains than methicillin-sensitive S. epidermidis (85 versus 70%). The attachment of 2 S. epidermidis strains to plastic surfaces was partially prevented by sagamicin subinhibitory concentrations. On the contrary, sub-MIC levels of norfloxacin increased the adhesion of S. epidermidis.
authors.affiliation: Laboratorio di Batteriologia e Micologia, Area Infettivologica, IRCCS S. Matteo, Pavia, Italia. pmarone@smatteo.pv.it
authors.forename: Piero
authors.initials: P
authors.lastname: Marone
authors.forename: Vincenzina
authors.initials: V
authors.lastname: Monzillo
authors.forename: Edoardo
authors.initials: E
authors.lastname: Carretto
authors.forename: Edward
authors.initials: E
authors.lastname: Haeusler
authors.forename: Elena
authors.initials: E
authors.lastname: Antoniazzi
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001422:Bacterial Adhesion / Q000187:drug effects; D004341:Drug Evaluation; D004352:Drug Resistance, Microbial; D005839:Gentamicins; D006801:Humans; D007910:Lenses, Intraocular; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000494:pharmacology
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D005839:Gentamicins; D009643:Norfloxacin; C037545:micronomicin