Determination of selenium in blood serum by inductively coupled plasma atomic emission spectrometry with pneumatic nebulization.
The possibility of determining selenium in blood serum using inductively coupled plasma emission spectrometry with conventional pneumatic nebulization was studied. A high-resolution spectrometer (SBW=6 pm) with laterally viewed ICP was employed. Analysis with conventional pneumatic nebulization could overcome laborious and demanding digestion, which is necessary for hydride generation. A pressure digestion with nitric acid at 160 degrees C was sufficient to decrease the carbon content in the serum sample to 5%-10% of its original value. Spectral interference of the CN band was observed and mathematically corrected. It was found that the carbon-induced selenium line emission enhancement occurred even under ICP optimized conditions. A method of determination was developed and applied to the analysis of blood serum. True limit of detection in real samples is 0.01-0.02 mg/L and the limit of quantification (RSD 10%) is 0.03-0.07 mg/L using Se I 196.090 nm line at an integration time of 10-2 s. The method was tested by analysis of porcine blood serum and the serum reference material Seronorm MI 0181.
Machát
Jirí
J
Laboratory of Plasma Sources for Chemical Analysis, Faculty of Science, Masaryk University Brno, Czech Republic.
Kanický
Viktor
V
Otruba
Vítezslav
V
D006801:Humans; D012015:Reference Standards; D012643:Selenium / Q000097:blood*; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*
D012643:Selenium