title: Detection of a human rotavirus with G12 and P[9] specificity in Thailand.
abstract: G12 rotavirus has not been detected anywhere in the world since the first detection of a human strain, L26 (G12, P1B[4]), in the Philippines in 1990. In this study, we isolated a human rotavirus (strain T152) with a VP7 of G12 specificity from the stool of an 11-month-old diarrheic patient in Thailand. The strain T152 exhibited a long RNA pattern and subgroup I specificity. In the comparison of the nucleotide and amino acid sequences of the VP7 gene of strain T152 with those of rotaviruses with different G type specificities, strain T152 showed the highest identity, 90.9 and 93.9%, respectively, to G12 prototype strain L26. In contrast, the VP4 gene of strain T152 showed the highest identity with P[9] specificity of human strains K8 and AU-1 and feline strains Cat2 and FRV-1, with homologies of 89.3 to 90.6% at the nucleotide level and 93.9 to 95.6% at the amino acid level. Thus, strain T152 was found to be a natural reassortant strain with G12 and P[9] specificities.
authors.affiliation: Enteric and Respiratory Viruses Laboratory, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand.
authors.forename: Y
authors.initials: Y
authors.lastname: Pongsuwanna
authors.forename: R
authors.initials: R
authors.lastname: Guntapong
authors.forename: M
authors.initials: M
authors.lastname: Chiwakul
authors.forename: R
authors.initials: R
authors.lastname: Tacharoenmuang
authors.forename: N
authors.initials: N
authors.lastname: Onvimala
authors.forename: M
authors.initials: M
authors.lastname: Wakuda
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013045:Species Specificity; D013785:Thailand
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus