[Sequencing and analysis of 16S rDNA sequences for P. cocovenenans subsp farinofermentans].
In order to fix the phylogenetic taxonomic position of P. cocovenenans subsp. farinofermentans and describe its relationships with other closed species, seven primers were designed to amplify and sequence the 16S rDNA of 4 strains of it by using a clonal sequencing or direct sequencing method. The 16S rDNA sequence of the 4 strains were aligned with the 16S rDNA sequences of other species in genus Burkholderia, and the polygenetic tree was produced by using a Clustal V and treeview software. The results showed that P. cocovenenans subsp. farinofermentans was in a high homology with Burkholderia gladioli and Burkholderia cocovenenans, and they formed an independent phylogenetic clustal of genus Burkholderia.
Jiao
Z
Z
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Liu
X
X
Yang
R
R
Meng
Z
Z
D004274:DNA, Recombinant; D004275:DNA, Ribosomal / Q000235:genetics; D011549:Pseudomonas / Q000145:classification*; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
D004274:DNA, Recombinant; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S