Comparison of the effects of HGF, BDNF, CT-1, CNTF, and the branchial arches on the growth of embryonic cranial motor neurons.
In the developing embryo, axon growth and guidance depend on cues that include diffusible molecules. We have shown previously that the branchial arches and hepatocyte growth factor (HGF) are growth-promoting and chemoattractant for young embryonic cranial motor axons. HGF is produced in the branchial arches of the embryo, but a number of lines of evidence suggest that HGF is unlikely to be the only factor involved in the growth and guidance of these axons. Here we investigate whether other neurotrophic factors could be involved in the growth of young cranial motor neurons in explant cultures. We find that brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and cardiotrophin-1 (CT-1) all promote the outgrowth of embryonic cranial motor neurons, while glial cell line-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) fail to affect outgrowth. We next examined whether HGF and the branchial arches had similar effects on motor neuron subpopulations at different axial levels. Our results show that HGF acts as a generalized rather than a specific neurotrophic factor and guidance cue for cranial motor neurons. Although the branchial arches also had general growth-promoting effects on all motor neuron subpopulations, they chemoattracted different axial levels differentially, with motor neurons from the caudal hindbrain showing the most striking response.
Naeem
Arifa
A
MRC Centre for Developmental Neurobiology, 4th Floor New Hunt's House, King's College, Guy's Campus, London SE1 1 UL, UK.
Abbas
Leila
L
Guthrie
Sarah
S
D000818:Animals; D001369:Axons / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D001934:Branchial Region / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002630:Chemotactic Factors / Q000502:physiology; D020934:Ciliary Neurotrophic Factor / Q000502:physiology*; D016207:Cytokines / Q000502:physiology*; D004622:Embryo, Mammalian; D017228:Hepatocyte Growth Factor / Q000502:physiology*; D009046:Motor Neurons / Q000145:classification / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor; D002630:Chemotactic Factors; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines; D017228:Hepatocyte Growth Factor; C092073:cardiotrophin 1