MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.
The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. CL4 cells were used to examine the dynamics of MYO1A (M1A; formerly BB myosin I) within the BB using GFP-tagged MIA (GFP-M1A), MIA motor domain (GFP-MDIQ), and tail domain (GFP-Tail). GFP-beta-actin (GFP-Actin) was used to assess actin dynamics within the BB. GFP-M1A, GFP-Tail, but not GFP-MDIQ localized to the BB, indicating that the tail is sufficient for apical targeting of M1A. GFP-Actin targeted to all the actin domains of the cell including the BB. Fluorescence recovery after photobleaching analysis revealed that GFP-M1A and GFP-Tail turnover in the BB is rapid, approximately 80% complete in <1 min. As expected for an actin-based motor, ATP depletion resulted in significant inhibition of GFP-M1A turnover yet had little effect on GFP-Tail exchange. Rapid turnover of GFP-M1A and GFP-Tail was not due to actin turnover as GFP-Actin turnover in the BB was much slower. These results indicate that the BB population of M1A turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. This rapidly exchanging pool of M1A envelops an actin core bundle that, by comparison, is static in structure.
Tyska
M J
MJ
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA. matthew.tyska.@yale.edu
Mooseker
M S
MS
D000199:Actins / Q000378:metabolism; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins; D006801:Humans; D007668:Kidney / Q000166:cytology; D007700:Kinetics; D018374:LLC-PK1 Cells; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008871:Microvilli / Q000378:metabolism*; D024461:Myosin Type I / Q000737:chemistry* / Q000378:metabolism*; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013347:Subcellular Fractions; D013552:Swine; D013997:Time Factors; D014162:Transfection
D000199:Actins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D024461:Myosin Type I