title: Telomerase activity in breast cancer in Western India (Gujarat).
abstract: The purpose of this study was to examine the association of telomerase activity with clinical and histopathological prognostic variables in primary breast cancer (n=64). Telomerase activity in breast cancer was also compared with that in benign (n=10) and non-malignant tissues (n=8; post-lumpectomy tissues histopathologically defined as containing no residual tumor). The parameter was assessed using the Telomerase PCR ELISA kit. Values above OD 0.120 were considered positive. Estrogen and progesterone receptors (ER and PgR) were assayed by the dextran-coated charcoal method and levels >10 fmol/mg cytosol protein were taken as positive. Telomerase activity was detected in 20% and 50% of the patients with benign lesions and primary breast cancer, respectively, and in 50% of post-lumpectomy breast tissues histopathologically defined as containing no residual tumor. Telomerase activity was present in all stages of breast carcinoma and showed a significant inverse correlation with lymph node status (p=0.006), lymphatic invasion (p=0.035) and necrosis (p=0.033). Moreover, when stage II patients were grouped according to nodal involvement, a trend towards significance was observed (p=0.055). No correlation was observed with ER and PgR. The results of our study suggest that telomerase activity might be associated with the presence of cancer cells. Furthermore, telomerase activation may occur early in breast cancer and may be periodically downregulated during subsequent tumor progression.
authors.affiliation: Division of Molecular Endocrinology, Department of Cancer Biology, Gujarat Cancer and Research Institute, Ahmedabad, India. gcriad@sancharnet.in
authors.forename: N G
authors.initials: NG
authors.lastname: Shah
authors.forename: T J
authors.initials: TJ
authors.lastname: Choksi
authors.forename: D D
authors.initials: DD
authors.lastname: Patel
authors.forename: H H
authors.initials: HH
authors.lastname: Vora
authors.forename: N
authors.initials: N
authors.lastname: Ghosh
authors.forename: T I
authors.initials: TI
authors.lastname: Trivedi
authors.forename: K A
authors.initials: KA
authors.lastname: Trivedi
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000201:enzymology*; D002454:Cell Differentiation; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007194:India; D008207:Lymphatic Metastasis; D008593:Menopause; D008875:Middle Aged; D011374:Progesterone / Q000378:metabolism; D011379:Prognosis; D011960:Receptors, Estrogen / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D019098:Telomerase / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D019098:Telomerase