title: The Activated Seven Lupus Anticoagulant (ASLA) assay: a new test for lupus anticoagulants (LAs). Evidence that some LAs are detectable only in extrinsic pathway-based assays.
abstract: Accurate and timely detection of lupus anticoagulants (LAs) is of diagnostic and prognostic importance due to the association of persistent LAs with thrombotic disease. In the present study, a sensitive and specific assay for LAs has been developed using recombinant activated factor VII to initiate in vitro coagulation. The Activated Seven Lupus Anticoagulant (ASLA) assay uses dilute brain-derived phospholipid in the screening test and a platelet neutralization procedure (PNP) in the confirmatory test. Tests are reported as ratios of patient clotting time to normal control clotting time and percentage correction by PNP assessed for abnormal ratios. Evaluation with 70 known LA-positive plasmas demonstrated higher detection rates than with individual assays from a wide range of commonly employed LA tests. The ASLA assay identified 61 of 70 (87%) known LAs, compared with 65.7% with the most sensitive of the other assays. The various LA assays were used to test 110 plasma samples from patients with thrombotic disease previously negative for LA. These experiments demonstrated that 18 of 110 (16.4%) contained LAs detectable only in extrinsic pathway-based assays, 10 of these by ASLA testing alone. ASLA testing showed high diagnostic precision and has the potential to make a significant contribution to LA detection.
authors.affiliation: Haemophilia Reference Centre, Centre for Thrombosis and Haemostasis, St. Thomas' Hospital, London, UK.
authors.forename: G W
authors.initials: GW
authors.lastname: Moore
authors.forename: M P
authors.initials: MP
authors.lastname: Smith
authors.forename: Y
authors.initials: Y
authors.lastname: Patel
authors.forename: G F
authors.initials: GF
authors.lastname: Savidge
mesh_terms: D001778:Blood Coagulation Disorders / Q000097:blood; D001780:Blood Coagulation Tests; D001923:Brain Chemistry; D015942:Factor VIIa / Q000494:pharmacology*; D006801:Humans; D016682:Lupus Coagulation Inhibitor / Q000097:blood*; D010743:Phospholipids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D016682:Lupus Coagulation Inhibitor; D010743:Phospholipids; D011993:Recombinant Fusion Proteins; D015942:Factor VIIa