Interleukin-1beta expression and phospholipase A(2) activation after intestinal ischemia/reperfusion injury.
The experiments were carried out to explore the interactions between IL-1 beta gene expression, protein level and phospholipase A(2)  PLA(2)   inhibition after intestinal ischemia/reperfusion injury. Using a rat intestinal ischemia/reperfusion injury model, after collecting the serum, lung lavage, abdomen cavity lavage and important organ tissue samples from control, injury and PLA(2) inhibitor treated groups, IL-1 beta level was measured by radioimmunoassay, and the mRNA expression of IL-1 beta and type II PLA (2)was determined by RT-PCR. After 6 h of injury, the IL-1 beta level in serum was significantly higher than that in the control group; an increase in IL-1 beta was also observed in abdomen cavity lavage 1 or 3 h after injury. IL-1 beta was significantly increased in liver tissue after injury, but was not changed obviously in the lung, kidney and intestinal tissues. IL-1 beta in the lung lavage was significantly higher than that of control group. The mRNA expression of IL-1 beta in lung tissue was increased after injury, but type II PLA(2) mRNA expression was decreased. There were different changes in IL-1 beta level and gene expression after treatment with PLA(2) inhibitor chloroquine, cyclo-oxidase inhibitor indomethacin, or PAF receptor antagonist SR27417 respectively after injury. All these results indicate that after intestinal ischemia/reperfusion injury, the IL-1 beta level and mRNA gene expression are significantly increased, however, the relationship among IL-1 beta, PLA(2) activation and its metabolite release remains to be further elucidated.
Yan
Guang-Tao
GT
Laboratory of Biochemistry, Basic Medicine Institute, General Hospital of PLA, Beijing 100853, yan301@263.net
Hao
Xiu-Hua
XH
Xue
Hui
H
Wang
Lu-Huan
LH
Li
Ying-Li
YL
Shi
Li-Ping
LP
D000818:Animals; D005260:Female; D015870:Gene Expression; D007375:Interleukin-1 / Q000096:biosynthesis / Q000378:metabolism*; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D010741:Phospholipases A / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*
D007375:Interleukin-1; D012333:RNA, Messenger; D010741:Phospholipases A