Correct identification of genes from serial analysis of gene expression tag sequences.
SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3' EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method.
Lee
Sanggyu
S
Department of Medicine, University of Chicago, 5841 S. Maryland, MC2115, Chicago, Illinois 60637, USA.
Clark
Terry
T
Chen
Jianjun
J
Zhou
Guolin
G
Scott
L Ridgway
LR
Rowley
Janet D
JD
Wang
San Ming
SM
D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans