Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants.
The alkali-soluble glucan of the yeast cell wall contains beta-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of beta-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-beta-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkalysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of beta-(1,6)-D-linkage than that from the wild-type.
Ha
C H
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Graduate School of Biotechnology, Korea University, Seoul, South Korea.
Lim
K H
KH
Kim
Y T
YT
Lim
S T
ST
Kim
C W
CW
Chang
H I
HI
D000468:Alkalies / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry; D005936:Glucans / Q000032:analysis* / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D015394:Molecular Structure; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D012995:Solubility
D000468:Alkalies; D005936:Glucans; D011135:Polysaccharides, Bacterial; D004693:Glucan Endo-1,3-beta-D-Glucosidase