A practical approach to the synthesis of hairpin polyamide-peptide conjugates through the use of a safety-catch linker.
Hairpin polyamides are high-affinity, sequence selective DNA binders. The use of a safety-catch linker for the solid phase synthesis of hairpin polyamides allows for easy preparation of derivatives ready for chemoselective ligation with unprotected peptides. Examples of ligations reported include thioether bond formation and thioester-mediated amide bond formation ('Native Chemical Ligation').
Fattori
Daniela
D
Istituto di Ricerche di Biologia Molecolare P. Angeletti (IRBM), Via Pontina Km 30.600, 00040 Pomezia, Rome, Italy.
Kinzel
Olaf
O
Ingallinella
Paolo
P
Bianchi
Elisabetta
E
Pessi
Antonello
A
D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009757:Nylons / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
D009757:Nylons; D010455:Peptides