[Propofol depresses c -fos expression of NOS neurons in the spinal cord of rats with inflammatory pain].
In formalin pain model, the effect of propofol on Fos expression in the spinal cord was examined by means of c -fos oncogene immunohistochemistry and NADPH-d histochemistry. Fos-like immunoreactive (FLI) neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were FLI/NOS double-labeled neurons. Most of the FLI or FLI/NOS double-labeled neurons were observed in the medial part of lamina  and the outer lamina  . Before or after injection of formain, i.p. injection of propofol significantly decreased the number of FLI and FLI/NOS double-labeled neurons in all laminae (P<0.05 or P<0.01). By single i.p. injection of propofol or normal saline, few FLI neurons were found in the spinal cord. The results suggest that the antinociceptive function of propofol is possibly involved in the depression of the NOS neurons in the spinal cord.
Yan
Ming
M
Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221006; yjy3001@263.net
Zhang
Li-Cai
LC
Dai
Ti-Jun
TJ
Zheng
Yin-Ming
YM
Duan
Shi-Ming
SM
D000818:Animals; D005260:Female; D005557:Formaldehyde; D008297:Male; D009474:Neurons / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010146:Pain / Q000139:chemically induced / Q000378:metabolism*; D015742:Propofol / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*
D016760:Proto-Oncogene Proteins c-fos; D005557:Formaldehyde; D019001:Nitric Oxide Synthase; D015742:Propofol