Resistance to unloading-induced three-dimensional bone loss in osteopontin-deficient mice.
Recent development in three-dimensional (3D) imaging of cancellous bone has made possible true 3D quantification of trabecular architecture. This provides a significant improvement in the measures available to study and understand the mechanical functions of cancellous bone. We recently reported that the presence of osteopontin (OPN) was required for the effects of mechanical stress on bone as OPN-null (OPN-/-) mice showed neither enhancement of bone resorption nor suppression of bone formation when they were subjected to unloading by tail suspension. However, in this previous study, morphological analyses were limited to two-dimensional (2D) evaluation. Although bone structure is 3D and thus stress effect should be evaluated based on 3D parameters, no such 3D morphological features underlying the phenomenon have been known. To elucidate the role of OPN in mediating mechanical stress effect based on true quantitative examination of bone, we evaluated 3D trabecular structures of hindlimb bones of OPN-/- mice after tail suspension. Tail suspension significantly reduced 3D parameters of bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and anisotropy and increased 3D parameters on trabecular separation (Tb.Sp) in wild-type mice. In contrast, these 3D parameters were not altered after tail suspension in OPN-/- mice. These data provided evidence that OPN is required for unloading-induced 3D bone loss.
Ishijima
Muneaki
M
Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Tsuji
Kunikazu
K
Rittling
Susan R
SR
Yamashita
Teruhito
T
Kurosawa
Hisashi
H
Denhardt
David T
DT
Nifuji
Akira
A
Noda
Masaki
M
D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density / Q000235:genetics*; D001862:Bone Resorption / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000502:physiology; D006614:Hindlimb; D021621:Imaging, Three-Dimensional; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000172:deficiency* / Q000235:genetics; D013314:Stress, Mechanical; D013623:Tail; D013977:Tibia / Q000000981:diagnostic imaging / Q000502:physiology
D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin