Detection of Escherichia coli in water by culture-based amperometric and luminometric methods.
The application of amperometric biosensor- and chemiluminiscence based methods for rapid detection of viable E. coli in water has been investigated. An amplification of the amperometric signal by a factor of 4 was obtained when the cellobiose dehydrogenase (CDH) biosensor was used instead of a plain graphite electrode for detection of b-galactosidase (b-GAL) activity at 22.5 degrees C. A linear correlation was demonstrated for detection time (DT) vs. initial concentrations (logarithmic units) of E. coli IT1 and E. coli in environmental samples, respectively, by use of the CDH biosensor or a chemiluminometric technique. The study has shown that an E. coli concentration > or = 10(4) cfu/100 mL in environmental samples was determined by the CDH biosensor within one working day. However, further reduction of the DT can be obtained, e.g. by increasing the signal amplification factor using other biosensors.
Nistor
C
C
Department of Analytical Chemistry, Lund University, Sweden.
Osvik
A
A
Davidsson
R
R
Rose
A
A
Wollenberger
U
U
Pfeiffer
D
D
Emnéus
J
J
Fiksdal
L
L
D015374:Biosensing Techniques / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004926:Escherichia coli / Q000201:enzymology / Q000302:isolation & purification*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism; D008163:Luminescent Measurements; D012722:Sewage / Q000382:microbiology*; D013997:Time Factors; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis*; D001616:beta-Galactosidase / Q000378:metabolism
D012722:Sewage; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; C019859:cellobiose-quinone oxidoreductase; D001616:beta-Galactosidase