[Determination of Cd, Cu, Pb, Hg by reversed-phase high performance liquid chromatography].
The chromatographic behaviors of Me(n+)-Dz (dithizone) have been studied with RP-HPLC. A method for the determination of Cd, Cu, Pb and Hg has been established. The chromatographic conditions were: column: Shim-pack CLC-ODS, 150 mm x 6.0 mm i.d.; mobile phase: V(methanol): V(water): V (chloroform) (containing volume fraction 1% triethylamine) = 80:12:8; flow rate: 1 mL/min; column temperature: 35 degrees C; detection wavelength: 254 nm. The linear ranges were from 0.01 mg/mL to 2.0 mg/mL with correlation coefficients of 0.9993-0.9998. The detection limits of Cd, Cu, Pb, Hg were from 2.4 micrograms/L to 5.0 micrograms/L. The RSDs were in the range from 1.8% to 9.7%, and the recoveries ranged from 94% to 103% (except Hg). The method has been applied to the analysis of hair.
Ding
C
C
Analysis and Testing Center, Sichuan Normal University, Chengdu, 610066.
Li
H
H
D002104:Cadmium / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003300:Copper / Q000032:analysis*; D006197:Hair / Q000737:chemistry; D006801:Humans; D007854:Lead / Q000032:analysis*; D008628:Mercury / Q000032:analysis*
D002104:Cadmium; D007854:Lead; D003300:Copper; D008628:Mercury