[Fluorescence probe two-hybridization technique and its application in medical genechips].
OBJECTIVE
To establish a new nucleic acid hybridization detection technique which may be used in medical genechips.


METHODS
The specific DNA fragment was detected by sequential two hybridization of fluorescence probe with template DNA and fixed DNA probe.


RESULTS
Fluorescence probe two-hybridization (FPTH) was applied to genechips for the detection of sex-transmitted pathogens from culture strains, and the results showed that the values of fluorescence density of the positive groups decreased remarkably when compared with those of the negative group. Both the sensitivity and specificity for detecting clinical samples are higher than 90%. There is no need of any additional reagent in hybridization procedure, and the hybridization detection can be accomplished in 40 minutes.


CONCLUSIONS
The FPTH technique is rapid, simple and reliable, it can also make the clinical detection process completely automatic and integrative.
Yang
Yuan
Y
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041 P. R. China. szzhang@mcwcums.com
Zhang
Sizhong
S
Wang
Jun
J
Yong
Gang
G
Xia
Qingjie
Q
Wu
Shaoqing
S
Sun
Yan
Y
D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016990:Ureaplasma urealyticum / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D005456:Fluorescent Dyes