title: Brine shrimp lethality assay of Bacopa monnieri.
abstract: Successive petroleum ether, chloroform, ethanol and water extracts, a saponin rich fraction (SRF) and bacoside A isolated from Bacopa monnieri were tested for brine shrimp lethality. Successive ethanol extracts and SRF showed potent activity. Bacoside A showed the maximum activity with a LC(50) of 38.3 microg/mL. The results confirmed the previous reports of an anticancer effect of Bacopa monnieri and suggest bacoside A as the active constituent.
authors.affiliation: Bioassay Unit, M/s Natural Remedies Pvt Ltd, 5B Veerasandra Indl Area, Hosur Road, Bangalore 561 229, India.
authors.forename: Prashanth
authors.initials: P
authors.lastname: D'Souza
authors.forename: Mundkinajeddu
authors.initials: M
authors.lastname: Deepak
authors.forename: Padmaja
authors.initials: P
authors.lastname: Rani
authors.forename: Sandhya
authors.initials: S
authors.lastname: Kadamboor
authors.forename: Anjana
authors.initials: A
authors.lastname: Mathew
authors.forename: Arun P
authors.initials: AP
authors.lastname: Chandrashekar
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D001156:Artemia / Q000187:drug effects*; D007194:India; D007928:Lethal Dose 50; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal
chemical_list: D010936:Plant Extracts