Anisylazoformylarginine: a superior assay substrate for carboxypeptidase B type enzymes.
Anisylazoformylarginine (CH(3)OC(6)H(4)-N=N-CO-Arg-OH) is rapidly hydrolyzed at the acyl-arginine linkage by the zinc-enzyme porcine carboxypeptidase B. The catalytic reaction is readily monitored spectrophotometrically by disappearance of the intense absorption (349 nm, epsilon 19,100) of the azo chromophore, which chemically fragments after substrate cleavage.
Mock
William L
WL
Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607-7061, USA. wlmock@uic.edu
Stanford
Daniel J
DJ
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism*; D002384:Catalysis; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013552:Swine
D001391:Azo Compounds; D001120:Arginine; D002268:Carboxypeptidases; D043424:Carboxypeptidase B