The protective effect of resveratrols on ischaemia-reperfusion injuries of rat hearts is correlated with antioxidant efficacy.
1. Dietary antioxidants are thought to be beneficial in reducing the incidence of coronary heart disease. In this study, we compared resveratrol and analogues on their antioxidation and free radical scavenging activities to their protective effects on ischaemia-reperfusion induced injuries of rat hearts. 2. Astringinin (3,3',4',5-tetrahydroxystilbene) was shown to be a more potent inhibitor than other analogues against Cu(2+)-induced LDL (low-density lipoprotein) oxidation, as measured by the formation of conjugated diene and TBARS (thiobarbituric acid-reactive substance) and by the electrophoretic mobility of the oxidized LDL. 3. Resveratrol (trans-3,4',5-trihydroxystilbene) and astringinin scavenged the stable free radical DPPH (1,1-diphenyl-2-picryl-hydrazyl) with an IC(0.200) of 7.1 and 4.3 microM, respectively. 4. Astringinin has a superoxide anion scavenging activity about 160 fold more potent than resveratrol. 5. After a 30 min global ischemia followed by 2 h reperfusion, astringinin (10 microM) significantly reduced infarct size, superoxide anion production and increased functional recovery of the coronary flow in Langendorff-perfused rat hearts. 6. The result showed there is a positive correlation between the anti-oxidation and cardioprotective activities among these phenolic compounds. Our finding together with the fact that astringinin is more water-soluble than resveratrol suggest that astringinin could potentially be used as an anti-oxidant and cardioprotective agent in biological systems.
Hung
Li-Man
LM
The Microcirculatory Laboratory, Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Su
Ming-Jai
MJ
Chu
Wing-Keung
WK
Chiao
Chin-Wei
CW
Chan
Wan-Fen
WF
Chen
Jan-Kan
JK
D000818:Animals; D001713:Biphenyl Compounds; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000517:prevention & control*; D010084:Oxidation-Reduction; D010851:Picrates / Q000494:pharmacology; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D011921:Rats, Inbred WKY; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D000077185:Resveratrol; D013267:Stilbenes / Q000627:therapeutic use*; D013481:Superoxides / Q000378:metabolism
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010851:Picrates; D020011:Protective Agents; D013267:Stilbenes; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D000077185:Resveratrol