title: Enzymatic synthesis of [3'-O-methyl-(3)H]malvidin-3-glucoside from petunidin-3-glucoside.
abstract: Malvidin-3-glucoside has been labeled by enzymatic synthesis in a single-step experiment. Catechol-O-methyl transferase catalyzed the B-ring methylation of petunidin-3-glucoside, and S-Adenosyl-L-[methyl-(3)H] methionine was the methyl donor. Solid phase extraction and preparative high-performance liquid chromatography were necessary to separate [3'-O-methyl-(3)H]malvidin-3-glucoside from an isomer and the starting material. The specific activity was 2.2 Ci mmol(-1), and the yield of incorporation was 1.1%. A possible application of the labeled material is the study of anthocyanin reactions in complex mixtures such as red wine where products are difficult to isolate and analyze.
authors.affiliation: Department of Viticulture and Enology, University of California, Davis, California 95616-8749, USA.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Zimman
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Waterhouse
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis* / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000378:metabolism*; D007553:Isotope Labeling; D008745:Methylation; D012436:S-Adenosylmethionine / Q000378:metabolism; D014316:Tritium; D014920:Wine / Q000032:analysis
chemical_list: D000872:Anthocyanins; D005960:Glucosides; D014316:Tritium; C455107:petunidin-3-glucoside; C458419:malvidin-3-glucoside; D012436:S-Adenosylmethionine; D002394:Catechol O-Methyltransferase