[Effects of smoking on interleukin-2 and its receptor system in human peripheral blood].
42 healthy male smokers and 42 healthy male nonsmokers matched in age and life style were strictly chosen into the study from Beijing urban and rural areas. Peripheral blood mononuclear cells(PBMCs) were separated and then stimulated by PHA under 37 degrees C, 5% CO2 condition. Immunochemical methods were applied in testing the synthesis of interleukin 2(IL-2) and the expression of its membrane receptor (mIL-2R) and the level of soluble receptor (sIL-2R) in serum. The results showed that when compared with nonsmokers, stimulated PBMC from smokers had significant higher percentage of mIL-2R positive cells (P < 0.05) in vitro. Levels of sIL-2R in serum of smokers were also significantly higher than those of nonsmokers (P < 0.05). The percentage of IL-2 secrete cells of smokers had higher tendency (P = 0.08). These data indicated that the immune function of smokers and nonsmokers are different concerning the IL-2 and its receptor system.
Jiao
L
L
Institute of Environmental Health Monitoring, Chinese Academy of Preventive Medicine, Beijing 100021, China.
Gan
D
D
Fang
Y
Y
D000328:Adult; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000097:blood*; D012907:Smoking / Q000009:adverse effects* / Q000276:immunology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2