Antisense of ATP synthase subunit e inhibits the growth of human hepatocellular carcinoma cells.
Differentially expressed genes between normal and hepatocellular carcinoma tissues were investigated using differential display. We identified a cDNA fragment that was overexpressed in cancer tissue. Homology analysis showed that the sequence was identical to human ATP synthase subunit e (hAS-e). Moreover, Northern blot analysis demonstrated that hAS-e was overexpressed in 10 of 11 (91%) specimens of hepatocellular carcinoma compared with corresponding normal tissues. We introduced antisense hAS-e into a human hepatocellular carcinoma BEL-7404 cell and found that downregulation of the hAS-e led to cell growth inhibition. It was also found that the antisense transfection could decrease the serum-stimulated activation of mitogen-activated protein kinase (MAP kinase). Together, the results suggest that antisense of hAS-e can inhibit cell proliferation through the MAP kinase pathway. Our data indicate that hAS-e may become a new target in gene therapy.
Ying
H
H
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, P. R. China.
Yu
Y
Y
Xu
Y
Y
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects*; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D008113:Liver Neoplasms; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D021122:Protein Subunits; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D020319:Oligodeoxyribonucleotides, Antisense; D021122:Protein Subunits; C465022:ATP5ME protein, human; D025261:Mitochondrial Proton-Translocating ATPases