Deoxyribozyme-based logic gates.
We report herein a set of deoxyribozyme-based logic gates capable of generating any Boolean function. We construct basic NOT and AND gates, followed by the more complex XOR gate. These gates were constructed through a modular design that combines molecular beacon stem-loops with hammerhead-type deoxyribozymes. Importantly, as the gates have oligonucleotides as both inputs and output, they open the possibility of communication between various computation elements in solution. The operation of these gates is conveniently connected to a fluorescent readout.
Stojanovic
Milan N
MN
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York 10032, USA. mns18@columbia.edu
Mitchell
Tiffany Elizabeth
TE
Stefanovic
Darko
D
D001483:Base Sequence; D015374:Biosensing Techniques; D021881:DNA, Catalytic / Q000737:chemistry*; D015195:Drug Design; D008128:Logic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry
D021881:DNA, Catalytic; D009841:Oligonucleotides