title: Adenovirus-mediated TGF-beta(1) gene transfer to human degenerative lumbar intervertebral disc cells.
abstract: OBJECTIVE
  To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor beta(1) (TGF-beta(1)) cDNA, and the expression of the encoded protein.
  
  
  METHODS
  Nucleus pulposus samples were surgically obtained from 8 patients with degenerative lumbar disc disease. The cells were cultured and directly infected by two adenoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence protein (EGFP) gene (marker gene) and Ad/CMV-TGF-beta(1) containing the potentially therapeutic TGF-beta(1) gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-beta(1)).
  
  
  RESULTS
  Culture cells transduced by Ad/CMV-EGFP showed specific green fluorescence under the fluoroscope and expression sustained for at least 4 weeks. When infected by Ad/CMV-TGF-beta(1), approximately 30% of cultured cells were stained brown (+) with TGF-beta(1) staining.
  
  
  CONCLUSIONS
  This study established the strategy of delivering a potentially therapeutic gene, TGF-beta(1), by using an adenoviral vector to human degenerative lumbar intervertebral disc cells.
authors.affiliation: Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China. drzhaojian@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Lianshun
authors.initials: L
authors.lastname: Jia
authors.forename: Deyu
authors.initials: D
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.forename: Xin
authors.initials: X
authors.lastname: Pan
authors.forename: Xin
authors.initials: X
authors.lastname: Jing
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000328:Adult; D005260:Female; D018014:Gene Transfer Techniques; D006801:Humans; D007403:Intervertebral Disc / Q000166:cytology*; D008159:Lumbar Vertebrae; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1
chemical_list: C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1