title: From cofactor to enzymes. The molecular evolution of pyridoxal-5'-phosphate-dependent enzymes.
abstract: The pyridoxal-5'-phosphate (vitamin B(6))-dependent enzymes that act on amino acid substrates have multiple evolutionary origins. Thus, the common mechanistic features of B(6) enzymes are not accidental historical traits but reflect evolutionary or chemical necessities. The B(6) enzymes belong to four independent evolutionary lineages of paralogous proteins, of which the alpha family (with aspartate aminotransferase as the prototype enzyme) is by far the largest and most diverse. The considerably smaller beta family (tryptophan synthase beta as the prototype enzyme) is structurally and functionally more homogenous. Both the D-alanine aminotransferase family and the alanine racemase family consist of only a few enzymes. The primordial pyridoxal-5'-phosphate-dependent protein catalysts apparently first diverged into reaction-specific protoenzymes, which then diverged further by specializing for substrate specificity. Aminotransferases as well as amino acid decarboxylases are found in two different evolutionary lineages, providing examples of convergent enzyme evolution. The functional specialization of most B(6) enzymes seems to have already occurred in the universal ancestor cell before the divergence of eukaryotes, archebacteria, and eubacteria 1500 million years ago. Pyridoxal-5'-phosphate must have emerged very early in biological evolution; conceivably, metal ions and organic cofactors were the first biological catalysts. To simulate particular steps of molecular evolution, both the substrate and reaction specificity of existent B(6) enzymes were changed by substitution of active-site residues, and monoclonal pyridoxal-5'-phosphate-dependent catalytic antibodies were produced with selection criteria that might have been operative in the evolution of protein-assisted pyridoxal catalysis.
authors.affiliation: Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland. christen@bioc.unizh.ch
authors.forename: P
authors.initials: P
authors.lastname: Christen
authors.forename: P K
authors.initials: PK
authors.lastname: Mehta
mesh_terms: D000591:Amino Acid Isomerases / Q000737:chemistry* / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D019143:Evolution, Molecular; D011732:Pyridoxal Phosphate / Q000737:chemistry* / Q000378:metabolism; D000637:Transaminases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D011732:Pyridoxal Phosphate; D000637:Transaminases; D000591:Amino Acid Isomerases