title: The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.).
abstract: The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.
authors.affiliation: Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland. bergmagn@hi.is
authors.forename: Bergljót
authors.initials: B
authors.lastname: Magnadóttir
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
authors.forename: Louise
authors.initials: L
authors.lastname: Royle
authors.forename: Cristina
authors.initials: C
authors.lastname: Colominas
authors.forename: David J
authors.initials: DJ
authors.lastname: Harvey
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002853:Chromatography, Liquid / Q000662:veterinary; D010450:Endopeptidases / Q000378:metabolism; D005399:Fishes / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000737:chemistry*; D007075:Immunoglobulin M / Q000097:blood / Q000737:chemistry*; D013058:Mass Spectrometry / Q000662:veterinary; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D009844:Oligosaccharides; C038601:trinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D010450:Endopeptidases