title: Immunohistochemical localization of acyl-CoA hydrolase/thioesterase multigene family members to rat epithelia.
abstract: Acyl-CoA hydrolases cleave acyl-CoA thioesters to free fatty acids and coenzyme A. The potency of these enzymes may serve to modulate cellular levels of acyl-CoAs to affect various cellular functions, including lipid metabolism. In this study, we investigated the tissue distribution of this multigene family of enzymes, focusing on cytosolic (CTE-I) and mitochondrial acyl-CoA thioesterases (MTE-I) in adult rats, using an anti-CTE-I antibody which recognizes both the isoforms. Western blotting detected them mainly in organs closely related to fatty acid oxidation, of which kidney contained the highest levels of both enzymes. Immunohistochemistry localized the enzymes primarily in the proximal tubules, where a large energy demand is expected and fatty acids represent a major fuel, correlating well with the intrarenal distribution of peroxisomal beta-oxidation. In situ hybridization suggested colocalization of CTE-I and MTE-I in the kidney. The immunoreactivity was also found in various epithelial tissues in the body, including Harderian gland and sebaceous gland. These results demonstrated the distribution of CTE-I and MTE-I in a wide variety of rat tissues, primarily characterized by an epithelial localization, being consistent with their involvement in fatty acid metabolism.
authors.affiliation: Department of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kuramochi
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Nishimura
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Takagi-Sakuma
authors.forename: Takafumi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Junji
authors.initials: J
authors.lastname: Kuroda
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hiratsuka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nagae
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Suga
authors.forename: Junji
authors.initials: J
authors.lastname: Yamada
mesh_terms: D002001:Adipose Tissue, Brown / Q000201:enzymology; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D005810:Multigene Family / Q000235:genetics*; D009206:Myocardium / Q000201:enzymology; D010170:Palmitoyl-CoA Hydrolase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000201:enzymology
chemical_list: D010170:Palmitoyl-CoA Hydrolase