title: Effects of shenmai injection on expression of TNF-alpha mRNA in peritoneal macrophages of scald mice.
abstract: OBJECTIVE
  To explore the effect of shenmai injection (SI) on expression of TNF-alpha mRNA in peritoneal macrophages (pMPhis) of scald mice.
  
  
  METHODS
  BALB/c mice were inflicted with 11% of body surface area III degree scald and injected intraperitoneally (ip) with SI daily for 5 days, and expression of TNF-alpha mRNA in pMPhis was determined by semi-quantitative RT-PCR.
  
  
  RESULTS
  In scald mice, the expression of TNF-alpha mRNA in pMPhis increased significantly, but it was reduced obviously (P < 0.01) after SI administration, while the livability was increased markedly (P < 0.05).
  
  
  CONCLUSIONS
  For scald mice, the cause of death at early stage might be related to the high expression of TNF-alpha mRNA in pMPhis and the use of SI can decrease the death rate.
authors.affiliation: Department of Immunology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China. Wangrt@hebmu.edu.cn
authors.forename: Runtian
authors.initials: R
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Dianwu
authors.initials: D
authors.lastname: Liu
mesh_terms: D000818:Animals; D002056:Burns / Q000235:genetics* / Q000401:mortality; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha