[Berberine Hydrochloride Reference Standard (Control 001) of National Institute of Health Sciences].
The raw material of Berberine Hydrochloride was examined for preparation of the "Berberine Hydrochloride Reference Standard". The analytical data obtained were: UV spectrum: lambda max, 420, 345, 263 and 228 nm and specific absorbance (E1ca1%) in ethanol at each lambda max, 155, 724, 796 and 820, respectively; IR spectrum, specific absorptions at 2844, 1635, 1569, and 1506 cm-1; and the spectrum of raw material was consistent with that of Standard (Control 941). Also, thin-layer chromatography, an impurity was detected; high-performance liquid chromatography, several impurities were detected. The amount of each impurity was estimated at less than 0.1% and the total amount of impurities was less than 0.2%. Based on the above results, the candidate material was authorized as the Berberine Hydrochloride Reference Standard (Control 001) of the National Institute of Health Sciences.
Koide
T
T
Iwata
M
M
Maekawa
K
K
Saito
H
H
Tanimoto
T
T
Okada
S
S
D001599:Berberine / Q000032:analysis / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D006077:Government Agencies; D007564:Japan; D010602:Pharmacopoeias as Topic / Q000592:standards; D012015:Reference Standards
D001599:Berberine