PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4.
A cDNA clone encoding GLORF-C4 was isolated from the WB strain, an assemblage A Giardia intestinalis. Interestingly, GLORF-C4 has been previously reported as an assemblage B-specific gene. Using two primers based on GLORF-C4 of the GS strain, a prototype assemblage B, GLORF-C4 gene was amplified from all the groups of G. intestinalis, and applied to detect the presence of cysts of G. intestinalis from faecal samples of cyst-passers. RFLP analysis of this PCR product successfully classified G. intestinalis into two distinct groups, assemblages A and B.
Yong
T
T
Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-752, Korea.
Han
K
K
Yang
H
H
Park
S
S
D000818:Animals; D001483:Base Sequence; D002681:China; D017931:DNA Primers; D005243:Feces / Q000469:parasitology; D017125:Genes, Protozoan; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000469:parasitology; D007723:Korea; D007812:Laos; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D016415:Sequence Alignment; D013045:Species Specificity
D017931:DNA Primers; C079594:GLORF-C4 protein, Giardia lamblia; D015800:Protozoan Proteins