L-delta-(alpha-Aminoadipoyl)-L-cysteine-D-valine synthetase: production of dipeptides containing valine residue at its C-terminus.
L-delta-(alpha-Aminoadipoyl)-L-cysteine-D-valine synthetase (ACVS) has been recently studied as a model enzyme for peptide synthetases. It was found that in the absence of alpha-aminoadipic acid but in the presence of several cysteine analogues it was incorporated into several analogue dipeptides upon incubation of the potential cysteine analogues with ACVS. [(14)C]Cysteine was incorporated into the[(14)C]cysteinyl-valine analogue dipeptides. Notably, [(14)C]valine incorporation in the presence of N-acylated cysteine analogues was observed. The alpha-aminoadipic acid activation site is influential, inhibitory or promotive, on the production of these putative dipeptide products. The production of dipeptide analogues, containing valine or analogues at the C-terminus, leads to the speculation that the biosynthetic direction of ACV could be from the C-terminus to the N-terminus.
Shiau
Chia-Yang
CY
Institute of Medical Science, National Defence Medical Centre, Taipei, 114, Republic of China. cys@ndmctsgh.edu.tw
Liu
Yu-Tien
YT
D015074:2-Aminoadipic Acid / Q000378:metabolism; D000164:Acremonium / Q000201:enzymology; D001665:Binding Sites / Q000502:physiology; D002250:Carbon Radioisotopes; D002384:Catalysis; D003545:Cysteine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D004151:Dipeptides / Q000096:biosynthesis* / Q000737:chemistry; D010453:Peptide Synthases / Q000378:metabolism*; D014633:Valine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
D002250:Carbon Radioisotopes; D004151:Dipeptides; D015074:2-Aminoadipic Acid; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase; D014633:Valine; D003545:Cysteine