Parameters of glucose and lipid metabolism in the male Göttingen minipig: influence of age, body weight, and breeding family.
The pig is useful as a model for human physiology and pathophysiology and could be an important supplement to the many available rodent models of diabetes mellitus. Due to their small size, Göttingen minipigs are especially suitable for long-term studies. The aim of the study reported here was to establish reference values for a range of glucose and lipid homeostasis parameters of interest that could be used to identify possible diabetes-prone male Göttingen minipig individuals, families, or age groups. Plasma samples from nonfed animals were analyzed for glucose, leptin, fructosamine, insulin, C-peptide, triglyceride, free fatty acids, and total cholesterol values. Breeding family had significant effects only on plasma triglyceride concentrations (P < 0.001). Plasma concentrations of glucose (P = 0.012), fructosamine (P < 0.001) and triglycerides (P < 0.001) increased significantly with age, whereas total cholesterol concentration decreased significantly (P = 0.001) with age. Age did not influence other parameters. In conclusion, glycemia and insulinemia increased with age and body weight, possibly indicating a small deterioration in insulin sensitivity with age. It is, therefore, hypothesized that older, compared to younger animals may be more useful in the development of a model of type-2 diabetes mellitus. Furthermore, on the basis of decrease in cholesterol concentration with age, animals fed ad libitum with possibly a high calorie diet might be even more useful in the development of a type-2 diabetes mellitus model.
Larsen
M O
MO
Department of Pharmacological Research I, Novo Nordisk A/S, Bagsvaerd, Denmark.
Rolin
B
B
Wilken
M
M
Carr
R D
RD
Svendsen
O
O
Bollen
P
P
D000375:Aging / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001835:Body Weight; D001947:Breeding; D002096:C-Peptide / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology; D004195:Disease Models, Animal; D019270:Fructosamine / Q000097:blood; D006706:Homeostasis; D006801:Humans; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood*; D008297:Male; D012016:Reference Values; D013552:Swine; D013556:Swine, Miniature / Q000033:anatomy & histology / Q000097:blood*; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D002096:C-Peptide; D007328:Insulin; D008055:Lipids; D014280:Triglycerides; D019270:Fructosamine