title: Making ends meet in genetic analysis using padlock probes.
abstract: Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single-nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tag-arrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable.
authors.affiliation: Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
authors.forename: Johan
authors.initials: J
authors.lastname: Banér
authors.forename: Maritha
authors.initials: M
authors.lastname: Mendel-Hartvig
authors.forename: Fredrik
authors.initials: F
authors.lastname: Dahl
authors.forename: Dan-Oscar
authors.initials: DO
authors.lastname: Antson
authors.forename: Mats
authors.initials: M
authors.lastname: Gullberg
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D004247:DNA / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011088:DNA Ligases / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D015342:DNA Probes; D012313:RNA; D004247:DNA; D011088:DNA Ligases