Multiplexed analysis of post-PCR fluorescence-labeled microsatellite alleles and statistical evaluation of their imbalance in brain tumors.
Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.
Yoshimoto
Koji
K
Division of Genome Analysis, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
Iwaki
Toru
T
Inamura
Takanori
T
Fukui
Masashi
M
Tahira
Tomoko
T
Hayashi
Kenshi
K
D022981:Allelic Imbalance; D001932:Brain Neoplasms / Q000097:blood / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D016000:Cluster Analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005456:Fluorescent Dyes; D005838:Genotype; D005909:Glioblastoma / Q000097:blood / Q000235:genetics*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats / Q000235:genetics*; D009837:Oligodendroglioma / Q000097:blood / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D004273:DNA, Neoplasm; D005456:Fluorescent Dyes