Application of solid/liquid extraction for the gravimetric determination of lipids in royal jelly.
Gravimetric lipid determination is a major parameter for the characterization and the authentication of royal jelly quality. A solid/liquid extraction was compared to the reference method, which is based on liquid/liquid extraction. The amount of royal jelly and the time of the extraction were optimized in comparison to the reference method. Boiling/rinsing ratio and spread of royal jelly onto the extraction thimble were identified as critical parameters, resulting in good accuracy and precision for the alternative method. Comparison of reproducibility and repeatability of both methods associated with gas chromatographic analysis of the composition of the extracted lipids showed no differences between the two methods. As the intra-laboratory validation tests were comparable to the reference method, while offering rapidity and a decrease in amount of solvent used, it was concluded that the proposed method should be used with no modification of quality criteria and norms established for royal jelly characterization.
Antinelli
Jean-François
JF
French Food Safety Agency (AFSSA), Unité Abeille, B.P. 111, F-06902 Sophia Antipolis Cedex, France. jf.antinelli@sophia.afssa.fr
Davico
Renée
R
Rognone
Catherine
C
Faucon
Jean-Paul
JP
Lizzani-Cuvelier
Louisette
L
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D008055:Lipids / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D000099219:Royal Jelly
D005227:Fatty Acids; D008055:Lipids; D012997:Solvents; D000099219:Royal Jelly