title: An immunoperoxidase monolayer assay for the detection of antibodies against swine influenza virus.
abstract: An immunoperoxidase monolayer assay (IPMA) has been developed to detect antibodies against swine influenza A virus (SIV) in pig sera. The test was evaluated by using sequential sera from pigs experimentally infected with H1N1 subtype of SIV. Two hundred field serum samples that had been examined by the hemagglutination inhibition (HI) test were also tested. Antibodies specific to SIV were detected as early as 3 days postinoculation (dpi) in the IPMA test as compared with 7 dpi by the HI test. Unlike HI, no serum treatment was required in the IPMA test. Regardless of the virus used in the test, IPMA detected antibodies to both H1N1 and H3N2 subtypes of SIV whereas HI detects antibodies against either H1N1 or H3N2, depending upon the virus used in the test. Results of this study indicate that IPMA is a useful test for screening of pig sera for SIV antibodies.
authors.affiliation: Department of Clinical and Population Sciences, University of Minnesota, St. Paul 55108, USA.
authors.forename: Kochakorn
authors.initials: K
authors.lastname: Direksin
authors.forename: HanSoo
authors.initials: H
authors.lastname: Joo
authors.forename: Sagar M
authors.initials: SM
authors.lastname: Goyal
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D009980:Influenza A virus / Q000276:immunology* / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral