Production and characterisation of monoclonal anti-idiotype antibody to Vibrio anguillarum.
Seven monoclonal anti-idiotype antibodies (mab2) were raised against mouse monoclonal antibody (mab1) 4A6. Identification of subclass showed that 1H5, 1D1, 2B12 and 2F12 belonged to IgG2b, 2H12 and 1H12 to IgG2a and lE10 to IgG3. The titres of these mab2 ascitic fluids ranged from 1 x 10(-4)-1 x 10(-6). The capacity of the mab2 to inhibit the binding between the corresponding rabbit antiserum and Vibrio anguillarum was investigated with the competitive inhibition ELISA. The results showed that mab2 1D1, 1E10, 1H5 and 1H12 were able to inhibit this binding. Another experiment demonstrated that mab2 1D1, 1E10 and 1H5 might induce Balb/c mice to produce Ab3 and these Ab3 competed the same antigen epitopes with Ab1. These results indicate that mab2 1D1, 1E10 and 1H5 are likely to represent an internal image of V. anguillarum and may thus be described as Ab2-beta anti-idiotype antibodies. In protection experiments, Japanese flounders vaccinated with mab21D1, 1E10 and 1H5 showed significantly enhanced survival from challenge with V. anguillarum. Thus. mab21D1, 1E10 and 1H5 may have use as idiotype vaccines for fish in aquaculture.
Yongjuan
Xia
X
Department of Histology and Embryology, Fourth Military Medical University, Xi'an, China.
Weiquan
Huang
H
Baocheng
Huang
H
Xiaohang
Jin
J
Rongqing
Zhang
Z
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D017756:Aquaculture; D001201:Ascites; D005260:Female; D005393:Fish Diseases / Q000517:prevention & control*; D005432:Flounder / Q000382:microbiology*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014611:Vaccination; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000517:prevention & control / Q000662:veterinary*
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal