Concentrations of cartilage oligomeric matrix protein in dogs with naturally developing and experimentally induced arthropathy.
OBJECTIVE
To assay concentrations of cartilage oligomeric matrix protein (COMP) in canine sera and synovial fluid (SF), to compare COMP concentrations in clinically normal dogs and dogs with joint disease, and to analyze changes in COMP concentrations in dogs with experimentally induced acute synovitis.


METHODS
69 control dogs without joint disease, 23 dogs with naturally occurring aseptic arthropathy, and 6 dogs with experimentally induced synovitis.


METHODS
Serum (n = 69) and SF (36) were obtained from control dogs. Samples of serum (n = 23) and SF (13) were obtained from dogs with naturally occurring aseptic arthropathy with or without radiographic features of osteoarthritis (OA). Serum and SF were obtained before and 1, 2, 3, and 7 days after induction of synovitis. The COMP concentrations were determined by use of an inhibition ELISA that had canine cartilage COMP and monoclonal antibody against human COMP.


RESULTS
Concentrations of COMP in serum and SF of control dogs were 31.3+/-15.3 and 298.7+/-124.7 microg/ml, respectively. In naturally occurring OA, COMP concentrations in serum (44.9+/-177 microg/ml) and SF (401.7+/-74.3 microg/ml) were significantly higher than corresponding concentrations in control dogs. The COMP concentration in SF peaked 24 and 48 hours after induction of synovitis, whereas concentration in serum peaked on day 3.


CONCLUSIONS
These results supported the hypothesis that COMP concentration in serum and SF of dogs may be altered after cartilage degradation or synovitis. Measurement of COMP concentrations can be useful when differentiating arthropathies in dogs.
Misumi
Kazuhiro
K
Department of Veterinary Surgery, Kagoshima University, Japan.
Vilim
Vladimir
V
Carter
Stuart D
SD
Ichihashi
Kyoko
K
Oka
Tatsuzo
T
Sakamoto
Hiroshi
H
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D064236:Cartilage Oligomeric Matrix Protein; D002358:Cartilage, Articular / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D016326:Extracellular Matrix Proteins / Q000097:blood / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism*; D007592:Joint Diseases / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D008297:Male; D064235:Matrilin Proteins; D013582:Synovial Fluid / Q000737:chemistry / Q000378:metabolism
D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; C577553:TSP5 protein, human