Recognition of plasmodium falciparum proteins by mannan-binding lectin, a component of the human innate immune system.
The mannan-binding lectin (MBL) is a serum protein, which is involved in the immune defence against viruses, bacteria and parasites. Children who have mutations in the MBL gene that lead to a MBL deficiency are more susceptible to infectious diseases and are more likely to suffer from severe malaria. In this report we investigate the interaction between MBL and the proteins of red blood cells infected with the parasite Plasmodium falciparum. Protein extracts were separated on MBL-sepharose columns. After the elution of bound material, the proteins were detected either by Western blot with human antibodies, or radioactive labelling with 35S-methionine or 3H-glucosamine. MBL recognises proteins of P. falciparum-infected erythrocytes that are immunogenic in humans, parasite-derived and glycosylated. Whether the proteins identified in the different assays are identical remains to be explored. MBL added to in vitro cultures of P. falciparum, however, does not inhibit parasite growth. The positive effect of MBL in the blood of malaria patients could be caused by detoxification of parasite products.
Klabunde
Jens
J
Department of Parasitology, Institute for Tropical Medicine, University of Tübingen, Germany.
Uhlemann
Anne-Catrin
AC
Tebo
Anne E
AE
Kimmel
Jürgen
J
Schwarz
Ralph T
RT
Kremsner
Peter G
PG
Kun
Jürgen F J
JF
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D020066:Merozoite Surface Protein 1 / Q000276:immunology; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism*
D000953:Antigens, Protozoan; D037601:Mannose-Binding Lectin; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium