Chemiluminescence assay for uric acid in human serum and urine using flow-injection with immobilized reagents technology.
A novel chemiluminescence (CL) flow sensor for the determination of uric acid in human urine and serum has been developed by using controlled-reagent-release technology. The reagents involved in the chemiluminescence (CL) reaction, luminol and periodate, are immobilized on anion-exchange resin packed in a column. After injection of water, chemiluminescence generated by released luminol and periodate in alkaline media is inhibited in presence of uric acid. By measuring the decreased chemiluminescence (CL) intensity the uric acid is sensed. The decreased response is linear in the 5.0-500.0 ng mL(-1) range, with a detection limit of 1.8 ng mL(-1). The flow sensor showed remarkable operational stability and could be easily reused for over 80 h with sampling frequency of 100 h(-1). The proposed sensor was applied to the determination of uric acid in human urine and serum, and monitoring metabolic uric acid in human urine with RSD less than 3.0%.
Song
Zheng-Hua
ZH
Department of Chemistry, Northwest University, Xi'an, China.
Hou
Shuang
S
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002138:Calibration; D017743:Equipment Reuse; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D008163:Luminescent Measurements; D008165:Luminol; D010504:Periodic Acid; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000097:blood* / Q000652:urine*
D007202:Indicators and Reagents; D007475:Ion Exchange Resins; D010504:Periodic Acid; D014527:Uric Acid; D008165:Luminol; C009288:metaperiodate