Atmospheric pressure laser desorption/chemical ionization mass spectrometry: a new ionization method based on existing themes.
We have designed and constructed an atmospheric pressure laser desorption/chemical ionization (AP-LD/CI) source that utilizes a laser pulse to desorb intact neutral molecules, followed by chemical ionization via reagent ions produced by a corona discharge. This source employs a heated capillary atmospheric pressure inlet coupled to a quadrupole ion trap mass spectrometer and allows sampling under normal ambient air conditions. Preliminary results demonstrate that this technique provides approximately 150-fold increase in analyte ions compared to the ion population generated by atmospheric pressure infrared matrix-assisted laser desorption/ionization (AP-IR-MALDI).
Coon
Joshua J
JJ
Department of Chemistry, University of Florida, Gainesville, FL 32611, USA.
McHale
Kevin J
KJ
Harrison
W W
WW
D000399:Air Pressure; D007202:Indicators and Reagents; D007834:Lasers; D012110:Reserpine / Q000032:analysis; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013134:Spiperone / Q000032:analysis
D007202:Indicators and Reagents; D012997:Solvents; D013134:Spiperone; D012110:Reserpine