Determination of 3,5,6-trichloro-2-pyridinol (TCP) in water by a continuous competitive immunoassay system based on the streptavidin-biotin interaction.
A competitive continuous immunoassay system for the determination of 3,5,6-trichloro-2-pyridinol (TCP), the major degradation product of the insecticide chlorpyrifos, in water is described. The immunoassay system is based on the transient retention of the specific LIB-MC2 monoclonal antibody anti-TCP as a biotinylated derivative using the streptavidin-biotin interaction. The permanent immobilization of streptavidin on controlled-pore-glass provides an adequate active support for the transient retention of the biotinylated monoclonal antibody anti-TCP. In a subsequent step, the immuno-competitive reaction between the biotinylated LIB-MC2 and the TCP/hapten-POD mixture takes place. This competitive assay relies on the determination of the biocatalytic action of peroxidase, retained in the active support, on a derivatization reaction which yields a fluorescent product. The method exhibits a determination range of 0.01-200 microg L(-1) of TCP (r2=0.9919, n=9) with a precision, expressed as RSD, lower than 4.2% and a sampling frequency of 3 h(-1). The approach has been applied to the determination of TCP in water with recoveries of 89.7-105.6%.
Delgado
Reyes F
RF
Analytical Chemistry Division, University of Córdoba, Spain.
Fernández
Romero J M
RJ
Luque
de Castro M D
de CM
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D001710:Biotin; D004784:Environmental Monitoring; D006540:Herbicides / Q000032:analysis; D007118:Immunoassay / Q000379:methods / Q000592:standards; D007306:Insecticides / Q000032:analysis*; D011728:Pyridones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019809:Streptavidin; D014867:Water / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis; D014881:Water Supply / Q000032:analysis
D000911:Antibodies, Monoclonal; D006540:Herbicides; D007306:Insecticides; D011728:Pyridones; D014874:Water Pollutants, Chemical; D014867:Water; C012587:3,5,6-trichloro-2-pyridinol; D001710:Biotin; D019809:Streptavidin