Electrofocusing procedure resolves phenolase of oak's bark in three distinct major isozymes showing cresolase and catecholase activities. Their pI are: 6, 7.8 and 8.2. Little change in ratio of the two activities occurs during isoelectric separation. The ultracentrifuged isozymes exhibit sedimentation constant similar to the crude enzyme phenolase.
Barthe
J P.
JP
Enzymologie et Métabolisme, Equipe de recherche associée au C.N.R.S. Centre de Physiologie Végétale, Université Paul Sabatier, 118, route de Narbonne 31, Toulouse, France
Gadal
P
P
Mise en evidence par focalisation isoelectrique de trois isoenzymes de la polyphenoloxydase chez Quercus pedunculata Ehrh.