title: Simultaneous determination of ondansetron and tropisetron in human plasma using HPLC with UV detection.
abstract: A rapid and sensitive HPLC method for the simultaneous quantitation of ondansetron and tropisetron, two serotonin (5-HT) receptor antagonists frequently used in treatment and prevention of nausea and emesis, is described. The procedure involves liquid-liquid extraction of human plasma with dichloromethane coupled with reversed-phase HPLC and UV detection. The lower limits of quantification (LOQ) were 0.62 ng/mL for ondansetron and 1.25 ng/mL or tropisetron. Intra- and inter-assay coefficients of variation ranged from 1.5 to 7.5% and 5.3 to 13.7%, respectively. The sensitivity and precision were sufficient for determination of plasma concentrations after therapeutic administration of both drugs and the method can be used for the estimation of pharmacokinetic parameters.
authors.affiliation: Institute of Clinical Pharmacology, University Medical Center Charité, Humboldt University Berlin, Berlin, Germany. steffen.bauer@charite.de
authors.forename: Steffen
authors.initials: S
authors.lastname: Bauer
authors.forename: Elke
authors.initials: E
authors.lastname: Störmer
authors.forename: Rolf
authors.initials: R
authors.lastname: Kaiser
authors.forename: Pierre-Benoit
authors.initials: PB
authors.lastname: Tremblay
authors.forename: Jürgen
authors.initials: J
authors.lastname: Brockmöller
authors.forename: Ivar
authors.initials: I
authors.lastname: Roots
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000097:blood* / Q000493:pharmacokinetics; D017294:Ondansetron / Q000097:blood* / Q000493:pharmacokinetics; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D000077526:Tropisetron
chemical_list: D007211:Indoles; D012702:Serotonin Antagonists; D017294:Ondansetron; D000077526:Tropisetron