title: Isolation of Arcanobacterium (Actinomyces) pyogenes from cases of feline otitis externa and canine cystitis.
abstract: Arcanobacterium pyogenes is a normal inhabitant of the mucous membranes of domestic animals, such as cattle, sheep, swine, and goats. It is also an opportunistic pathogen in these animals, where it causes a variety of purulent infections involving the skin, joints, and visceral organs. Two recent cases of isolation of A. pyogenes from companion animals are reported. In the first case, a cat presented with a chronic otitis externa, from which A. pyogenes was isolated in pure culture. The second case involved a dog with a urinary tract infection, where A. pyogenes was isolated from urine as the predominant bacterial species. In both cases, the A. pyogenes isolates were presumptively identified by macrobiochemical tests, and then their identities were confirmed by polymerase chain reaction analysis and 16S rRNA gene sequencing.
authors.affiliation: Department of Veterinary Science and Microbiology, The University of Arizona, Tucson 85721, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Billington
authors.forename: K W
authors.initials: KW
authors.lastname: Post
authors.forename: B H
authors.initials: BH
authors.lastname: Jost
mesh_terms: D000190:Actinomyces / Q000302:isolation & purification* / Q000472:pathogenicity; D000193:Actinomycetales Infections / Q000662:veterinary*; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003556:Cystitis / Q000382:microbiology* / Q000662:veterinary*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D008297:Male; D008969:Molecular Sequence Data; D010032:Otitis Externa / Q000382:microbiology* / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial