title: Chiral separation of gemifloxacin in sodium-containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis.
abstract: Chiral crown ether, (+)-(18-crown-6)-tetracarboxylic acid (18C6H(4)), is an effective chiral selector for resolving enantiomeric primary amines owing to the difference in affinities between 18C6H(4) and each of the amine enantiomers. In addition to the destacking effect of sodium ion in the sample solution, the strong affinity of sodium ion to the polyether ring of crown ether is unfavorable to chiral capillary electrophoresis using 18C6H(4) as a chiral selector. In this report, the chiral separation of gemifloxacin dissolved in a saline sample matrix using 18C6H(4) was investigated. Adding a chelating agent, ethylenediaminetetraacetic acid (EDTA), to the run buffer greatly improved the separation efficiencies and peak shapes. The successful chiral separation of gemifloxacin in a urinary solution was demonstrated for both capillary and microchip electrophoresis.
authors.affiliation: School of Electrical Engineering and Computer Science, Seoul National University, Seoul 151-744, Korea.
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Cho
authors.forename: Kook-Nyung
authors.initials: KN
authors.lastname: Lee
authors.forename: Yong-Kweon
authors.initials: YK
authors.lastname: Kim
authors.forename: Jaeho
authors.initials: J
authors.lastname: Jang
authors.forename: Doo Soo
authors.initials: DS
authors.lastname: Chung
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D043844:Crown Ethers; D004492:Edetic Acid; D019075:Electrophoresis, Capillary / Q000379:methods*; D004988:Ethers, Cyclic / Q000737:chemistry; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D006801:Humans; D015394:Molecular Structure; D009287:Naphthyridines / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine; D012964:Sodium
chemical_list: D000890:Anti-Infective Agents; D043844:Crown Ethers; D004988:Ethers, Cyclic; D024841:Fluoroquinolones; D009287:Naphthyridines; C090732:18-crown-6 2,3,11,12-tetracarboxylic acid; D004492:Edetic Acid; D012964:Sodium; D000077735:Gemifloxacin