[Effect of antioxidant vitamins on lipid peroxide injury of aortic endothelial cells induced by oxidatively modified low-density lipoprotein in vitro].
The effect of antioxidant vitamins including vitamin E, vitamin C and beta-carotene on lipid peroxidation injury of aortic endothelial cells induced by oxidatively modified low density lipoprotein (oxLDL) were observed. Bovine aortic endothelial cells were incubated in culture media with antioxidant vitamins for 12 hours, and then with oxLDL (0.1 g/L) for 24 hours respectively. The results showed that the malondialdehyde (MDA) content in culture media of the groups with oxLDL was significantly higher than those of groups with vitamin E, vitamin C and beta-carotene alone. The number of HL60 monocyte cells adhered to bovine aortic endothelial cells was obviously higher in oxLDL groups than those in groups with only antioxidant vitamins. This study indicated that antioxidant vitamins are protective on vascular from injury induced by oxLDL, including decreasing lipid peroxidation and reducing adherence of monocyte cells on endothelia and seemed to be promising in the prevention of atherosclerosis.
He
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Department of Health Administration, Shanghai Second Medical University, Shanghai 200025, China.
Wang
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F
Cai
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D000818:Animals; D001011:Aorta / Q000166:cytology; D001205:Ascorbic Acid / Q000494:pharmacology*; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D018922:HL-60 Cells; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology; D019207:beta Carotene / Q000494:pharmacology
D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D019207:beta Carotene; D014810:Vitamin E; D008315:Malondialdehyde; D001205:Ascorbic Acid