title: Busulfan-induced loss of Ph chromosome in chronic myeloid leukemia.
abstract: We report a 44-yr-old female with Philadelphia chromosome positive chronic myeloid leukemia who was initially treated with busulfan, and clinical remission has been achieved. After 4 yr, low-dose busulfan therapy was started again and induced bone marrow aplasia. The patient spontaneously has recovered from aplasia, and complete cytogenetic remission with loss of Ph+ chromosome in bone marrow has been achieved. However, reverse-transcription-polymerase chain reaction analysis showed presence of the bcr-abl transcript in bone marrow.
authors.affiliation: Clinic of Internal Medicine, Clinical Center Dr Dragisa Misovic, Belgrade, Yugoslavia. radojkov@EUnet.yu
authors.forename: M
authors.initials: M
authors.lastname: Radojkovic
authors.forename: S
authors.initials: S
authors.lastname: Ristic
authors.forename: M
authors.initials: M
authors.lastname: Colovic
authors.forename: B
authors.initials: B
authors.lastname: Todoric
authors.forename: K
authors.initials: K
authors.lastname: Krtolica
mesh_terms: D000328:Adult; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D001853:Bone Marrow; D002066:Busulfan / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D016313:Genes, abl; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000235:genetics*; D010677:Philadelphia Chromosome; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018906:Antineoplastic Agents, Alkylating; D004273:DNA, Neoplasm; D002066:Busulfan