[The antioxidative effect of procyanidins from pine bark in vitro].
OBJECTIVE
To assess the antioxidative effect of procyanidins from pine bark on free radical damage.


METHODS
Hemolysis, Malonaldehyde(MDA) level of mice liver homogenates, the conformation changes of irradiated plasmid PUC18 were used as indexes.


RESULTS
Procyanidins could reduce the hemolysis degree of human RBC induced by H2O2 significantly(P < 0.01), reduce the MDA level of mice liver homogenate initiated by VitC/Fe2+ remarkably(P < 0.01), and reduce the degree of single-strain break of plamid PUC18 induced by 60Co gamma significantly(P < 0.01).


CONCLUSIONS
Procyanidins had good antioxidative function. It can prevent RBC membrane, plasmid DNA suffering from oxygen free radical damage in vitro.
Lin
C
C
Medical College of Jinan University, Guangzhou 510632.
Jiang
J
J
Wu
M
M
Yan
Y
Y
Zhou
Y
Y
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D008099:Liver / Q000737:chemistry; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D028223:Pinus / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D010957:Plasmids / Q000032:analysis; D044945:Proanthocyanidins
D000975:Antioxidants; D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; C017674:procyanidin; D008315:Malondialdehyde; D002392:Catechin