Direct polymerase chain reaction detection of Campylobacter spp. in poultry hatchery samples.
A rapid, sensitive, and specific polymerase chain reaction (PCR) assay was developed for the direct detection of Campylobacter in environmental samples from hatcheries. PCR, with a set of primers specific for the Campylobacter flaA short variable region (SVR), detected the presence of Campylobacter in both fluff and eggshell samples; however, a determination of whether the organism was living or dead could not be made. Conventional cultural methods detected no Campylobacter from the same samples. An additional benefit of the direct PCR assay is it allows for the production of a product that can be sequenced to provide further epidemiologic information.
Hiett
Kelli L
KL
United States Department of Agriculture, Agricultural Research Service, Poultry Microbiological Safety Research Unit, Athens, GA 30604-5677, USA.
Cox
Nelson A
NA
Stern
Norman J
NJ
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D001483:Base Sequence; D002167:Campylobacter / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D002645:Chickens; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004528:Egg Shell / Q000382:microbiology; D005241:Feathers / Q000382:microbiology; D005408:Flagellin / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D005408:Flagellin; C067138:flaA protein, bacteria