[Effects and applications of ascorbic acid on the proliferation of Chlamydia trachomatis].
Two hundred and eighty-eight samples of patients with nongonococcal urethritis (NGU) and prostatitis were detected by cell culture and immunofluorescence assay for Chlamydia trachomatis (CT) and the effects of ascorbic acid(vitC) on the formation rate of inclusion of CT in positive samples were also studied. The results showed that the formation rate of inclusion of CT was 29.5% when the concentration of vitC was 5 micrograms.ml-1. The difference between test group and control group which contained cycloheximide in the media was insignificant (P > 0.05). The results suggested that vitC was a kind of nutrient needed for CT. The proliferation of CT in the cell can be promoted by vitC. We can replace cycloheximide by vitC in McCoy cell culture to detect clinic samples with CT.
Yu
P
P
Department of Immunology, Hunan Medical University, Changsha 410078.
Wei
C
C
Hu
F
F
Gong
D
D
Shi
X
X
D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000494:pharmacology*; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D002479:Inclusion Bodies; D008297:Male; D008875:Middle Aged; D011472:Prostatitis / Q000382:microbiology; D014526:Urethritis / Q000382:microbiology; D002575:Uterine Cervicitis / Q000382:microbiology
D001205:Ascorbic Acid