Partial purification and determination of molecular weights of glyoxalases by filtration on dextran gel.
Glyoxalase I and glyoxalase II (EC. 4.4.1.5 and EC 3.1.2.6) were separated by gel filtration on Sephadex G-75 and G-100. This simple procedure permitted also the partial purification of glyoxalase II. The purification coefficient in a single run from supernatant from beef liver was about 1 : 30 compared with 1 : 15 after the fifth step of purification with classical methods.
Jerzykowski
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Department of Biochemistry, Medical Academy, Zabrze, Poland
Matuszewski
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Winter
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R