title: Modelling the consequences of receptor-G-protein promiscuity.
abstract: Many G-protein-coupled receptors interact with more than one type of G protein, giving rise to extreme variability in the effects of receptor activation, depending on, for example, receptor density and desensitization, efficacy of agonists, and availability of specific G proteins. This leads to errors in interpretation of data. To facilitate understanding the consequences of receptor-G-protein promiscuity, we use two simplified models to simulate such consequences. Applied to the regulation of adenylyl cyclase and phosphoinositidase, the models predict seemingly paradoxical situations and explain some phenomena that, at first sight, might seem to require the induction of agonist-specific (G-protein-selective) receptor conformations.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220, Prague, Czech Republic. tucek@biomed.cas.cz
authors.forename: Stanislav
authors.initials: S
authors.lastname: Tucek
authors.forename: Pavel
authors.initials: P
authors.lastname: Michal
authors.forename: Viktorie
authors.initials: V
authors.lastname: Vlachová
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism / Q000502:physiology; D000818:Animals; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism / Q000502:physiology; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism / Q000502:physiology; D019204:GTP-Binding Proteins / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000378:metabolism* / Q000502:physiology
chemical_list: D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019205:GTP-Binding Protein alpha Subunits, Gs; D000262:Adenylyl Cyclases