Potentiometric phosphate-sensing system utilizing phosphate-binding protein.
A phosphate-detection system has been developed which uses phosphate-binding protein (PBP) from Escherichia coli. PBP was immobilized on a sheet of nitrocellulose membrane by cross-linking and the membrane potential of the immobilized PBP was measured. The response time of the system to phosphate was 5 min. The response was selective to phosphate among other anions. Under optimum conditions 0.1-1.5 mmol L(-1) phosphate can be determined with this system.
Kubo
Izumi
I
Department of Bioengineering, Faculty of Engineering, Soka University, Hachioji, Tokyo, Japan
D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D003101:Collodion / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D017736:Ion-Selective Electrodes; D008567:Membranes, Artificial; D028044:Phosphate-Binding Proteins / Q000737:chemistry*; D010710:Phosphates / Q000032:analysis*; D011199:Potentiometry
D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D003101:Collodion