Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique.
METHODS
Dynamic fluorescence quenching is a technique that may overcome some of the limitations associated with measurement of tissue partial oxygen tension (PO2). We compared this technique with a polarographic Eppendorf needle electrode method using a saline tonometer in which the PO2 could be controlled. We also tested the fluorescence quenching system in a rodent model of skeletal muscle ischemiahypoxia.


RESULTS
Both systems measured PO2 accurately in the tonometer, and there was excellent correlation between them (r(2) = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected.


CONCLUSIONS
Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.
Shaw
Andrew D
AD
Department of Critical Care Medicine, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA. ashaw@mdanderson.org
Li
Zheng
Z
Thomas
Zach
Z
Stevens
Craig W
CW
D000818:Animals; D015687:Cell Hypoxia; D003627:Data Interpretation, Statistical; D005336:Fiber Optic Technology; D005453:Fluorescence; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D008962:Models, Theoretical; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism; D010101:Oxygen Consumption; D010313:Partial Pressure; D011048:Polarography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014065:Tonometry, Ocular
D010100:Oxygen