title: Early recurrence of benign meningioma correlates with expression of mini-chromosome maintenance-2 protein.
abstract: We have investigated the potential utility of monoclonal antibodies against mini-chromosome maintenance-2 protein (Mcm2) in predicting meningioma recurrence. MCM proteins are members of the DNA-binding prereplicative complex and are essential for eukaryotic DNA replication. They are present throughout the cell cycle, but are down-regulated in quiescence and cell differentiation, making them specific markers of proliferating cells. We analysed 10 benign meningiomas that subsequently recurred within a 5-year period, together with 20 matched non-recurrent benign meningiomas. There was no significant correlation between histological subtype, mitotic count or Ki-67 labelling index and tumour recurrence. We observed that whilst the average Mcm2 labelling index (LI) of the tumour section as a whole (LI(Ave)) is not significantly different between recurrent and nonrecurrent meningiomas, the Mcm2 labelling index in the area of highest proliferative activity within the tumour section (LI(Max)) is significantly higher in recurrent meningiomas (p < 0.0001). Seven out of the 10 recurrent meningiomas displayed a Mcm2 LI((Max) greater than 30%, compared to 0 out of 20 for non-recurrent tumours. In conclusion, these results suggest that analysis of Mcm2 expression may facilitate identification of patients with a high risk of meningioma recurrence, for whom adjuvant radiotherapy may be of benefit.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: D P J
authors.initials: DP
authors.lastname: Hunt
authors.forename: A
authors.initials: A
authors.lastname: Freeman
authors.forename: L S
authors.initials: LS
authors.lastname: Morris
authors.forename: N G
authors.initials: NG
authors.lastname: Burnet
authors.forename: K
authors.initials: K
authors.lastname: Bird
authors.forename: T W
authors.initials: TW
authors.lastname: Davies
authors.forename: R A
authors.initials: RA
authors.lastname: Laskey
authors.forename: N
authors.initials: N
authors.lastname: Coleman
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis*; D004261:DNA Replication; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008579:Meningioma / Q000737:chemistry* / Q000473:pathology; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D008940:Mitotic Index; D009363:Neoplasm Proteins / Q000032:analysis*; D009364:Neoplasm Recurrence, Local / Q000737:chemistry* / Q000473:pathology; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D011379:Prognosis
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D064111:Minichromosome Maintenance Complex Component 2