Oxybuprocaine induces a false-positive response in immunochromatographic SAS Adeno Test.
OBJECTIVE
To investigate whether a solution of oxybuprocaine hydrochloride, 0.4%, results in a false-positive response in an immunochromatographic SAS Adeno Test.


METHODS
Experimental study.


METHODS
Physiologic saline and 2% lidocaine.


METHODS
Each chemical (100 microl) was diluted in a transport medium. Five drops (200 microl) of the resultant solution were dispensed into the round sample well of a test device. Fifteen samples were tested in each group.


METHODS
Ten minutes after the start of the test, a colored line in the "specimen" portion of the test membrane was visually read as positive or negative by a masked technician.


RESULTS
No positive reaction was observed in the control groups (physiologic saline and lidocaine). A false-positive reaction was observed in six samples (33.3%) in the oxybuprocaine group. The positive rate was significantly higher in the oxybuprocaine group compared with those in control groups (P = 0.0062, Fisher's extract probability test).


CONCLUSIONS
Oxybuprocaine may induce a false-positive reaction in an immunochromatographic SAS Adeno Test. We recommend the use of lidocaine, instead of oxybuprocaine, for local anesthesia in taking eye swabs from patients with suspected adenovirus infection.
Hoshino
Takeshi
T
Department of Ophthalmology, Kurashiki Central Hospital, Kurashiki, Japan.
Takanashi
Taiji
T
Okada
Morio
M
Uchida
Sunao
S
D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000382:microbiology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000772:Anesthesia, Local / Q000379:methods*; D000779:Anesthetics, Local / Q000008:administration & dosage*; D002845:Chromatography; D003236:Conjunctivitis, Viral / Q000175:diagnosis* / Q000382:microbiology; D003951:Diagnostic Errors; D005189:False Positive Reactions; D006801:Humans; D008012:Lidocaine / Q000008:administration & dosage; D011343:Procaine / Q000008:administration & dosage* / Q000031:analogs & derivatives*
D000779:Anesthetics, Local; D011343:Procaine; D008012:Lidocaine; C005298:benoxinate