Antioxidant potential of a novel tetrapeptide derivative in isoproterenol-induced myocardial necrosis in rats.
A novel tetrapeptide derivative Boc-Lys(Boc)-Arg-Asp-Ser(tbu)-OtBu (PEP1261) has been tested in vivo in isoproterenol (ISO) hydrochloride (HCl)-induced myocardial necrosis in rats. ISO x HCl induces myocardial necrosis in rats which is accompanied by the distinct increase in heart weight, marked electrocardiographic changes, increase in the levels of serum marker enzymes and lipid peroxides and decrease in the levels of antioxidants. PEP1261 (5 mg/kg body weight i.p.) pre- and post-treatment effectively decreases serum marker enzyme levels, while the electrocardiographic changes get restored towards normalcy. PEP1261 also inhibits the action of the free radicals toxicity by increasing the levels of antioxidants and histological studies confirm the above findings. This study shows that PEP1261 could serve as an excellent cardioprotective agent possessing membrane-stabilizing action.
Manikandan
Panchatcharam
P
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, India.
Sumitra
Miriyala
M
Kumar
Dilly Ashok
DA
Gayathri
Chandrasekar
C
Arutselvan
Natarajan
N
Manohar
Bhakthavatsalam Murali
BM
Puvanakrishnan
Rengarajulu
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004562:Electrocardiography; D005260:Female; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D007545:Isoproterenol / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000097:blood / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009336:Necrosis; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D008054:Lipid Peroxides; D009842:Oligopeptides; D007545:Isoproterenol