title: [Tissue engineering study on chitosan-gelatin/hydroxyapatite composite scaffolds--osteoblasts culture].
abstract: OBJECTIVE
  To investigate the behavior of rat calvarial osteoblasts cultured on chitosan-gelatin/hydroxyapatite (CS-Gel/HA) composite scaffolds.
  
  
  METHODS
  The rat calvarial osteoblasts (the 3rd passage) were seeded at a density of 1.01 x 10(6) cells/ml onto the CS-Gel/HA composite scaffolds having porosity 85.20%, 90.40% and 95.80%. Cell number was counted after cultured for 3 days, 1 week, 2 weeks and 3 weeks. Cell proliferation, bone-like tissue formation, and mineralization were separately detected by HE, von Kossa histological staining techniques.
  
  
  RESULTS
  The CS-Gel/HA composite scaffolds supported the attachment of seeded rat calvarial osteoblasts. Cells proliferated faster in scaffold with higher porosity 90.40% and 95.80% than scaffold with lower porosity 85.20%. The osteoblasts/scaffold constructs were feasible for mineral deposition, and bone-like tissue formation in 3 weeks.
  
  
  CONCLUSIONS
  This study suggests the feasibility of using CS-Gel/HA composite scaffolds for bone tissue engineering.
authors.affiliation: Research Institute of Polymeric Materials, Tianjin University, Tianjin, P. R. China 300072.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yu-Ji
authors.initials: YJ
authors.lastname: Yin
authors.forename: Kang-De
authors.initials: KD
authors.lastname: Yao
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Wang
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Ming-fang
authors.initials: MF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives*; D005109:Extracellular Matrix; D005780:Gelatin; D006882:Hydroxyapatites; D010006:Osteoblasts / Q000502:physiology*; D051381:Rats; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; D002686:Chitin; D005780:Gelatin