title: Effect of antibiotics on in vitro and in vivo avian cartilage degradation.
abstract: Antibiotics are used in the livestock industry not only to treat disease but also to promote growth and increase feed efficiency in less than ideal sanitary conditions. However, certain antibiotic families utilized in the poultry industry have recently been found to adversely affect bone formation and cartilage metabolism in dogs, rats, and humans. Therefore, the first objective of this study was to determine if certain antibiotics used in the poultry industry would inhibit in vitro cartilage degradation. The second objective was to determine if the antibiotics found to inhibit in vitro cartilage degradation also induced tibial dyschondroplasia in growing broilers. Ten antibiotics were studied by an avian explant culture system that is designed to completely degrade tibiae over 16 days. Lincomycin, tylosin tartrate, gentamicin, erythromycin, and neomycin sulfate did not inhibit degradation at any concentration tested. Doxycycline (200 microg/ml), oxytetracycline (200 microg/ml), enrofloxacin (200 and 400 microg/ml), ceftiofur (400 microg/ml), and salinomycin (10 microg/ml) prevented complete cartilage degradation for up to 30 days in culture. Thus, some of the antibiotics did inhibit cartilage degradation in developing bone. Day-old chicks were then administered the five antibiotics at 25%, 100%, or 400% above their recommended dose levels and raised until 21 days of age. Thiram, a fungicide known to induce experimental tibial dyschondroplasia (TD), was given at 20 ppm. Birds were then killed by cervical dislocation, and each proximal tibiotarsus was visually examined for TD lesions. The results showed that none of these antibiotics significantly induced TD in growing boilers at any concentration tested, whereas birds given 20 ppm thiram had a 92% incidence rate.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Peters
authors.forename: R M
authors.initials: RM
authors.lastname: Fulton
authors.forename: K D
authors.initials: KD
authors.lastname: Roberson
authors.forename: M W
authors.initials: MW
authors.lastname: Orth
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D002356:Cartilage / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002357:Cartilage Diseases / Q000139:chemically induced / Q000517:prevention & control / Q000662:veterinary*; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D010009:Osteochondrodysplasias / Q000139:chemically induced / Q000517:prevention & control / Q000662:veterinary*; D011201:Poultry Diseases / Q000139:chemically induced* / Q000517:prevention & control; D013893:Thiram / Q000009:adverse effects; D013977:Tibia / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D013893:Thiram; D003094:Collagen