title: Anisylazoformylarginine: a superior assay substrate for carboxypeptidase B type enzymes.
abstract: Anisylazoformylarginine (CH(3)OC(6)H(4)-N=N-CO-Arg-OH) is rapidly hydrolyzed at the acyl-arginine linkage by the zinc-enzyme porcine carboxypeptidase B. The catalytic reaction is readily monitored spectrophotometrically by disappearance of the intense absorption (349 nm, epsilon 19,100) of the azo chromophore, which chemically fragments after substrate cleavage.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607-7061, USA. wlmock@uic.edu
authors.forename: William L
authors.initials: WL
authors.lastname: Mock
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Stanford
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D043424:Carboxypeptidase B; D002268:Carboxypeptidases / Q000378:metabolism*; D002384:Catalysis; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013552:Swine
chemical_list: D001391:Azo Compounds; D001120:Arginine; D002268:Carboxypeptidases; D043424:Carboxypeptidase B