An assessment of the effect of Ginkgo Biloba EGb 761 on ischemia reperfusion injury of intestine.
OBJECTIVE
The aim of this study was to determine the effect of Ginkgo Biloba (EGb 761) on reperfusion injury of the small bowel.


METHODS
Forty-eight male 200-250 g Spraque-Dawley rats in six groups were used to determine the biochemical and histopathological changes after a 30-min ischemia and 30-min reperfusion. Pre-treatment with 50 mg/kg EGb 761 (Tebofortan, Karlsruhe-Germany) or 10-mL/kg saline was administered intravenously in the treatment and control groups. The superior mesenteric artery was occluded distal to the right colic artery and collateral arcades were ligated to provide complete ischemia. Ischemia was determined by the existence of pulseless or pale color of the small intestine. The return of the pulses and the reestablishment of the pink color were assumed to be the reperfusion of the intestine. Rats that were administered Egb 761 and saline were subjected to laparotomy, ischemia, or ischemia-reperfusion procedures. Mucosal lesions were graded from 0 to 5 in histopathological examination. Malondialdehyde and myeloperoxidase levels of the intestinal mucosa were measured.


RESULTS
No significant difference was noted between the control and treatment groups regarding the histopathological changes. Although malonyldialdehyde and myeloperoxidase levels of the reperfusion + EGb 761 group were slightly higher than the laparotomy + saline group, they were significantly lower than the reperfusion + saline group.


CONCLUSIONS
We concluded that EGb 761 pre-treatment before ischemia-reperfusion decreased malondialdehyde and myeloperoxidase levels and attenuated the mucosal damage.
Pehlivan
Mevlüt
M
Department of General Surgery, Abant Izzet Baysal University, Düzce School of Medicine, 14450 Konuralp, Düzce, Turkey.
Dalbeler
Yesim
Y
Hazinedaroglu
Selçuk
S
Arikan
Yüksel
Y
Erkek
A Bülent
AB
Günal
Omer
O
Türkçapar
Nuran
N
Türkçapar
Ahmet Gökhan
AG
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D007413:Intestinal Mucosa / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology; D007421:Intestine, Small / Q000098:blood supply* / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D023421:Models, Animal; D009195:Peroxidase / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000503:physiopathology; D000096983:Ginkgo Extract
D000975:Antioxidants; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D009195:Peroxidase; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract