title: Estimation of drug-protein binding parameters on assuming the validity of thermodynamic equilibrium.
abstract: This contribution focuses the reader's attention on the pitfalls usually emerging during the phase of evaluation of experimental data of drug-protein binding studies. To overcome the occurrence of problem(s) apparently defying solution, the concept of "affinity spectra" is recommended to be implemented for data evaluation. A (general) "binding study protocol" is also suggested, which can prevent the formation of inadequate conclusions and the generation of unrealistic drug-protein binding parameters.
authors.affiliation: Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava. exfasolt@savba.sk
authors.forename: Ladislav
authors.initials: L
authors.lastname: Soltés
authors.forename: Mojmír
authors.initials: M
authors.lastname: Mach
mesh_terms: D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D004364:Pharmaceutical Preparations; D011506:Proteins