title: The molecular characterization of maize B chromosome specific AFLPs.
abstract: The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGG
authors.affiliation: The College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Zhong Xia
authors.initials: ZX
authors.lastname: Qi
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.forename: Xiu Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Cheng Bin
authors.initials: CB
authors.lastname: Chen
authors.forename: Wen Qin
authors.initials: WQ
authors.lastname: Song
authors.forename: Rui Yang
authors.initials: RY
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002503:Centromere / Q000737:chemistry; D032461:Chromosomes, Plant / Q000737:chemistry*; D017124:Conserved Sequence; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D016615:Telomere / Q000737:chemistry; D003313:Zea mays / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers