A simplified method for the HPLC resolution of alpha-carotene and beta-carotene (trans and cis) isomers.
A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of alpha-carotene, all-trans-beta-carotene, 9-cis-beta-carotene, 13-cis-beta-carotene and 15-cis-beta-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determination of carotenoids in a dried Dunaliella salina extract, but it could be also applied to other organic matrices such as eggs.
Bononi
M
M
Laboratorio Analisi Alimenti, Facoltà di Agraria, Università degli Studi di Milano, Italy.
Commissati
I
I
Lubian
E
E
Fossati
A
A
Tateo
F
F
D000818:Animals; D002138:Calibration; D002338:Carotenoids / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004530:Egg Yolk / Q000737:chemistry; D056890:Eukaryota / Q000737:chemistry; D012015:Reference Standards; D013237:Stereoisomerism; D019207:beta Carotene / Q000032:analysis
D019207:beta Carotene; D002338:Carotenoids