Delandin, a chitinase-like protein with antifungal, HIV-1 reverse transcriptase inhibitory and mitogenic activities from the rice bean Delandia umbellata.
An antifungal protein with a chitinase-like N-terminal sequence, designated delandin, was isolated from the rice bean. The protein exhibited a molecular weight of 28 kDa and was adsorbed on both blue Affi-Gel and SP-Toyopearl. It exerted antifungal action toward Mycosphaerella arachidicola, Botrytis cinerea, Fu- sarium oxysporum, Rhizoctonia solani, and Colletotrichum gossypii and inhibited the activity of human immunodeficiency virus 1 reverse transcriptase. The protein inhibited translation in rabbit reticulocyte lysate with a low potency. It elicited a mitogenic response from mouse splenocytes.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
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D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000378:metabolism*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000302:isolation & purification / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D000935:Antifungal Agents; D008934:Mitogens; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase