Production of superoxide anion by head-kidney leucocytes of Indian major carps immunised with bacterins of Aeromonas hydrophila.
On contact with micro-organisms or any other antigenic particles which are recognised as phagocytosable, the leucocytes of vertebrates raise their oxygen consumption suddenly (respiratory burst). The consumed oxygen is then converted into reactive oxygen species (ROS). An effort has been made in the present study to estimate the production of superoxide anion, one of the ROS, by the resident as well as activated head-kidney leucocytes of Indian major carps. Activation was accomplished by immunisation with formalin killed (FK) and heat killed (HK) whole cell bacterins of Aeromonas hydrophila. In the ex vivo experiment conducted, activated leucocytes yielded a significant increase in O.D. values for nitroblue tetrazolium (NBT) reduction reaction, reflecting an increase in superoxide anion production, from day 1 post immunisation. Of the three species of Indian major carps immunised, Catla catla showed the greatest production of superoxide anion, followed by Labeo rohita and then by Cirrhinus mrigala. The enhancement of superoxide anion production of leucocytes by immunisation justifies the role of immunisation in the microbicidal defence mechanism of fish.
John
M Basheera
MB
Department of Animal Science, School of Life Sciences, Bharathidasan University, Tamil Nadu, India.
Chandran
M R
MR
Aruna
B V
BV
Anbarasu
K
K
D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002347:Carps / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D007202:Indicators and Reagents; D007668:Kidney / Q000166:cytology / Q000276:immunology; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000502:physiology; D013481:Superoxides / Q000378:metabolism*; D015164:Vaccines, Inactivated / Q000276:immunology
D001428:Bacterial Vaccines; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D015164:Vaccines, Inactivated; D013481:Superoxides; D009580:Nitroblue Tetrazolium