title: Determination of triamterene and its main metabolite hydroxytriamterene sulfate in human urine by capillary electrophoresis using ultraviolet absorbance and laser-induced fluorescence detection.
abstract: Two capillary electrophoresis methods have been developed for the direct determination of triamterene and its main metabolite hydroxytriamterene sulfate in human urine. Analytes were detected using conventional UV detection as well as laser-induced fluorescence (LIF) detection with an HeCd-laser operating at a wavelength of 325 nm. The results of both detection techniques were compared. Indeed, the limit of quantification was eightfold lower using LIF detection (50 ng/ml) in comparison to UV detection (400 ng/ml). As no interference due to endogenous urine compounds was observed, direct urine analysis was feasible. Analysis was very simple and fast-one run could be performed within less than 10 min (CE-UV method) and 2.5 min (CE-LIF method), respectively. Both assays were fully validated and applied to urine samples from a human volunteer. The results of the application of the CE-LIF method to human urine samples are presented in this publication.
authors.affiliation: Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Horstkötter
authors.forename: Susan
authors.initials: S
authors.lastname: Kober
authors.forename: Hildegard
authors.initials: H
authors.lastname: Spahn-Langguth
authors.forename: Ernst
authors.initials: E
authors.lastname: Mutschler
authors.forename: Gottfried
authors.initials: G
authors.lastname: Blaschke
mesh_terms: D004232:Diuretics / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007834:Lasers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D014223:Triamterene / Q000031:analogs & derivatives* / Q000652:urine*
chemical_list: D004232:Diuretics; C026475:hydroxytriamterene sulfate ester; D014223:Triamterene