N(6)-Adenine DNA-methyltransferase in wheat seedlings.
The N(6)-adenine DNA-methyltransferase was isolated from the vacuolar vesicle fraction of wheat coleoptiles. In the presence of S-adenosyl-L-methionine the enzyme de novo methylates the first adenine residue in the TGATCA sequence in the single- or double-stranded DNA substrates but it prefers single-stranded structures. Wheat adenine DNA-methyltransferase (wadmtase) is a Mg(2+)- or Ca(2+)-dependent enzyme with a maximum activity at pH 7.5-8.0. Wadmtase seems to be responsible for mitochondrial DNA modification that might be involved in the regulation of replication of mitochondria in plants.
Fedoreyeva
Larisa I
LI
Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow 119899, Russia.
Vanyushin
Boris F
BF
D002118:Calcium / Q000494:pharmacology; D002850:Chromatography, Gel; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000201:enzymology; D004247:DNA / Q000737:chemistry; D019175:DNA Methylation; D004272:DNA, Mitochondrial / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009841:Oligonucleotides / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D018520:Plant Shoots / Q000737:chemistry / Q000201:enzymology*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry* / Q000302:isolation & purification; D013379:Substrate Specificity; D014908:Triticum / Q000201:enzymology*
D004272:DNA, Mitochondrial; D009841:Oligonucleotides; D010940:Plant Proteins; D004247:DNA; C427203:calf thymus DNA; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D008274:Magnesium; D002118:Calcium