Phosphorylated neurofilaments and SNAP-25 in cultured SH-SY5Y neuroblastoma cells.
Components of the extracellular matrix (ECM) of mammals have profound effects on the behavior and differentiation of many different cell types. Here, we report the results of biochemical and immunocytochemical investigations of the expression of SNAP-25 and phosphorylated neurofilament proteins (NFs) by cells grown on coverslips, cells cultured in EHS-ECM gels, and cells in situ in rat brain. SNAP-25 and phosphorylated NFs were detected by immunofluorescence in all these environments but were not detectable by Western analysis in extracts of cells grown on coverslips. The results support the interpretation that EHS-ECM induces differentiation of SH-SY5Y cells in culture and suggest this system as a model system for study of nerve tissue formation and repair.
Glass
Tracy L
TL
Department of Biology, Southwest Texas State University, 601 University Drive, San Marcos, TX 78666-4616, USA.
Raabe
Timothy D
TD
García
Dana M
DM
Koke
Joseph R
JR
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002451:Cell Compartmentation / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000378:metabolism* / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000378:metabolism*; D009432:Neural Crest / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009447:Neuroblastoma; D016900:Neurofilament Proteins / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D050825:Synaptosomal-Associated Protein 25; D014407:Tumor Cells, Cultured
D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C494208:SNAP25 protein, human; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; C051774:neurofilament protein H; C061195:neurofilament protein M