Evaluation of rapid staining techniques for cytologic diagnosis of intracranial lesions.
OBJECTIVE
To evaluate 4 rapid supravital stains and 3 preparation techniques for use in the intraoperative diagnosis of intracranial lesions.


METHODS
10 dogs and 1 cat euthanatized for intracranial lesions.


METHODS
Specimens were taken from lesions and slides prepared, using 3 techniques: touch impression, medium-pressure impression, or smear preparation. Preparations were then stained with 4 stains: modified Wright stain, May-Grünwald-Giemsa, toluidine blue, and zynostain and examined in a blinded randomized fashion. Cytologic diagnosis was compared with histopathologic diagnosis and classified on the basis of identification of the pathologic process and specific diagnosis into the following categories: complete correlation, partial correlation, or no correlation.


RESULTS
An overall diagnostic accuracy of 81% (107/132) was achieved on the basis of a combination of partial and complete correlation. Of the stains examined, modified Wright stain appeared to be most accurate, with complete correlation in 17 of 33 (52%) specimens and partial correlation in 12 of 33 (36%) specimens. Of the preparation methods, touch preparation and smear preparation provided the most accurate results, with an overall diagnostic accuracy of 82% (36/44) for both methods. However, smear preparations appeared to be of greater diagnostic value, with fewer nondiagnostic specimens, compared with touch preparations.


CONCLUSIONS
Cytologic preparations provide a useful diagnostic tool for the intraoperative diagnosis of intracranial lesions. All stains examined yielded promising results, the most accurate of which appeared to be the modified Wright stain. The smear preparation appeared to be the preparation method of greatest diagnostic value.
Long
Sam N
SN
Department of Small Animal Clinical Studies, University of Glasgow Veterinary School, Bearsden, UK.
Anderson
T James
TJ
Long
Fenella H A
FH
Johnston
Pamela E J
PE
D000230:Adenocarcinoma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000473:pathology; D002415:Cats; D003584:Cytological Techniques / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D008577:Meningeal Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D008579:Meningioma / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary