title: Prevalence of causative organisms in corneal ulcers seen at Sekuru Kaguvi Eye Unit, Harare, Zimbabwe.
abstract: OBJECTIVE
  To investigate the prevalence of organisms causing corneal ulcers, determine frequency of ocular risk factors and assess the value of Gram stain in initial identification of pathogens.
  
  
  METHODS
  A descriptive cross sectional study.
  
  
  METHODS
  Sekuru Kaguvi Eye Unit at Parirenyatwa Group of Hospitals in Harare, Zimbabwe.
  
  
  METHODS
  We enrolled 43 patients with corneal ulcers for the study. Demographic data was obtained, measurement of visual acuity was done by Snellen's chart and a Topcon Slit Lamp was used to determine the characteristics of ulcers for all the patients. Corneal scrapings were obtained and examined by microscopy and culture methods to determine etiologic organisms. Positivity rates between Gram stain and culture methods were compared.
  
  
  METHODS
  Prevalence of different bacterial isolates in corneal ulcers, risk factors to infection and laboratory methods used for detection.
  
  
  RESULTS
  Gram-positive cocci were the most prevalent organisms found (54%) followed by E. coli (18%). Pseudomonas, Klebsiella and Narcodia constituted about 9% each. Trauma, use of traditional eye medicine and previous viral disease were the common risk factors.
  
  
  CONCLUSIONS
  Bacteria were the only organisms identified and Gram-positive cocci were the most common isolates. Gram stain of corneal smears was not reliable in initial recognition of offending pathogens.
authors.affiliation: Sakubva Eye Unit, Mutare Provincial Hospital, P O Box 30, Mutare, Zimbabwe. wanimena@mutare.icon.co.zw
authors.forename: M G
authors.initials: MG
authors.lastname: Wani
authors.forename: N A
authors.initials: NA
authors.lastname: Mkangamwi
authors.forename: S
authors.initials: S
authors.lastname: Guramatunhu
mesh_terms: D000328:Adult; D003320:Corneal Ulcer / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control; D003430:Cross-Sectional Studies; D015818:Eye Infections, Bacterial / Q000453:epidemiology* / Q000382:microbiology* / Q000517:prevention & control; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D012307:Risk Factors; D015030:Zimbabwe / Q000453:epidemiology