title: Positron emission tomography and ex vivo and in vitro autoradiography studies on dopamine D2-like receptor degeneration in the quinolinic acid-lesioned rat striatum: comparison of [11C]raclopride, [11C]nemonapride and [11C]N-methylspiperone.
abstract: With [11C]raclopride,[11C]nemonapride and [11C]N-methylspiperone, degeneration of dopamine D2-like receptors in the unilaterally quinolinic acid-lesioned rats was evaluated by positron emission tomography (PET) and ex vivo and in vitro autoradiography. PET showed a decreased uptake of [11C]raclopride in the lesioned striatum, but an increased uptake of [11C]nemonapride and [11C]N-methylspiperone despite a decreased binding in vitro. Ex vivo autoradiography showed an increased accumulation of the three ligands in the cortical region overlying the injured striatum, probably enlarging PET signals. PET has the limited potential for evaluating the receptor degeneration in the present animal model.
authors.affiliation: Positron Medical Center, Tokyo Metropolitan Institute of Gerontology, 1-1 Naka-cho, Itabashi-ku, 172-0022, Tokyo, Japan. ishiwata@pet.tmig.or.jp
authors.forename: Kiichi
authors.initials: K
authors.lastname: Ishiwata
authors.forename: Nobuo
authors.initials: N
authors.lastname: Ogi
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Hayakawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Umegaki
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Nagaoka
authors.forename: Keiichi
authors.initials: K
authors.lastname: Oda
authors.forename: Hinako
authors.initials: H
authors.lastname: Toyama
authors.forename: Kazutoyo
authors.initials: K
authors.lastname: Endo
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.forename: Michio
authors.initials: M
authors.lastname: Senda
mesh_terms: D000818:Animals; D001345:Autoradiography; D001549:Benzamides / Q000493:pharmacokinetics*; D018492:Dopamine Antagonists / Q000493:pharmacokinetics*; D005947:Glucose / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D017378:Quinolinic Acid / Q000633:toxicity*; D020891:Raclopride / Q000493:pharmacokinetics*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000187:drug effects / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism; D013134:Spiperone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D014055:Tomography, Emission-Computed / Q000379:methods*
chemical_list: D001549:Benzamides; D018492:Dopamine Antagonists; D009498:Neurotoxins; D019275:Radiopharmaceuticals; D017448:Receptors, Dopamine D2; D011963:Receptors, GABA-A; D020891:Raclopride; D013134:Spiperone; C039167:3-N-methylspiperone; D017378:Quinolinic Acid; D005947:Glucose; C030265:nemonapride