title: [Determination of isorhamnetine and quercetin in flavone hippophaes tablets by HPLC method].
abstract: OBJECTIVE
  A method was established for the determination of isorhamnetine and quercetin in flavone hippophaes tablets by HPLC.
  
  
  METHODS
  The HPLC system consisted of Diamonsil C18 column (250 mm x 4.6 mm, 5 microns), mobile phase of methanol-0.02 mol/L phosphoric acid (54:46), column temperature at 40 degrees C, with detection at 368 nm.
  
  
  RESULTS
  The linear range of isorhamnetine and quercetin were 6.5-32 micrograms/ml (r = 0.9996) and 2.5-12.4 micrograms/ml (r = 0.9991). The average recoveries were 99.4% and 99.8%, respectively. The within-day precision RSD were 1.22% and 1.32% (n = 6), and the day-to-day precision RSD were 1.07% and 1.35% (n = 5), respectively.
  
  
  CONCLUSIONS
  This method is simple and accurate. It is suitable for the preparation quality control.
authors.affiliation: Fujian Provincial Institute for Drug Control, Fuzhou 350001.
authors.forename: S
authors.initials: S
authors.lastname: Weng
authors.forename: J
authors.initials: J
authors.lastname: Lian
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044948:Flavonols; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis*; D013607:Tablets
chemical_list: D004365:Drugs, Chinese Herbal; D044948:Flavonols; D013607:Tablets; C047368:3-methylquercetin; D011794:Quercetin