Mass spectrometric analysis of cyclofenil and its metabolites in human urine.
Using gas chromatography/electron impact-mass spectrometry (GC/EI-MS) and high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS), the structures of cyclofenil metabolites in human urine have been assigned. The hydroxyl metabolites liberated from the glucuronide conjugates after acid hydrolysis were characterized as the trimethylsilyl (O-TMS) derivatives using GC/MS. The conjugate glucuronide forms were detected without hydrolysis by HPLC/MS. Cyclofenil was not observed in urine. Tentative structures for the two metabolites are proposed.
Myung
Seung-Woon
SW
Doping Control Center, Korea Institute of Science and Technology, P.O. Box 131, Seoul 136-791, Korea. swmyung@kist.re.kr
Min
Hye-Ki
HK
Kim
Dong-Hyun
DH
Kim
Myungsoo
M
Cha
SuJin
S
Yoo
Eun-Ah
EA
Yim
Yong-Hyeon
YH
D000328:Adult; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003506:Cyclofenil / Q000652:urine*; D005260:Female; D005300:Fertility Agents, Female / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male
D005300:Fertility Agents, Female; D003506:Cyclofenil