[Study on apoptosis of human gastric adenocarcinoma cell SGC-7901 induced by aining in vitro and the cell cycle].
OBJECTIVE
To study the inducing apoptosis effect of Aining on human gastric adenocarcinoma cell in vitro.


METHODS
Agarose gel electrophoresis (AGE) and flow cytometry (FMC) techniques were adopted to study the inducing apoptosis effect of Ainging on the human gastric adenocarcinoma cell.


RESULTS
The human gastric adenocarcinoma cell's DNA was digested into fragments which gave rise to a characteristic "DNA Ladder" on agarose gel electrophoresis, while the percentage of apoptotic cells was 43.5%. But the blank group had neither DNA ladder, nor apoptotic peak. Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty.


CONCLUSIONS
Aining can induce human gastric adenocarcinoma cell apoptosis in vitro.
Su
M
M
National Engineering Research Center for Mordernization of TCM, Zhuhai 519020.
Lü
X
X
Li
H
H
Wan
B
B
Liu
J
J
D000230:Adenocarcinoma / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D006801:Humans; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal