[Group-specific antibodies against zearalenone and its metabolites and synthetic analogs].
Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of carboxymethyloxime-zearalanone (CMO-ZAN) and carboxymethyloxime-zearalenone (CMO-ZEN). Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters and carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/alpha-zearalenone and zearalanol/alpha-zearalanol) based on heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml).
Burkin
A A
AA
All-Russia Research Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, 123022 Russia.
Kononenko
G P
GP
Soboleva
N A
NA
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D007124:Immunoenzyme Techniques / Q000379:methods; D008297:Male; D011817:Rabbits / Q000097:blood / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology; D015025:Zearalenone / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D014614:Vaccines, Synthetic; D015025:Zearalenone