Decline in reproductive success, sex reversal, and developmental alterations in Japanese medaka (Oryzias latipes) after continuous exposure to octylphenol.
Japanese medaka (Oryzias latipes) were continuously exposed to concentrations of 0, 2, 20, and 50 microg/L octylphenol, 0.01 per thousand dimethyl sulfoxide, and 100 ng/L 17beta-estradiol from 2 to 4 h postfertilization until maturity. Exposure to > or =2 microg/L octylphenol caused a 20-30% increase in mortality both before and after hatch. Growth was significantly decreased in fish exposed to 50 microg/L octylphenol and in the positive control. Sex ratio changed from 42:58 (females:males) in controls to 57:40 at 50 microg/L octylphenol, and 3 and 4% of the medaka exposed to 50 microg/L octylphenol and 17beta-estradiol developed testes-ova. In cross-mating experiments, mating of exposed males with control females resulted in an up to 11% lower fertilization rate than in controls. Results indicate that continuous exposure to octylphenol causes a broad variety of reproductive effects, which can be correlated with the xenoestrogenic potential of octylphenol.
Knörr
Susanne
S
Aquatic Ecology and Toxicology Group, Department of Zoology I, University of Heidelberg, Im Neuenheimer Feld 230, D-69120 Heidelberg, Germany.
Braunbeck
Thomas
T
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D006066:Gonads / Q000002:abnormalities / Q000187:drug effects; D057850:Hermaphroditic Organisms; D008136:Longevity / Q000187:drug effects; D008297:Male; D009990:Oryzias / Q000502:physiology*; D010636:Phenols / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D019849:Sex Determination Processes; D012744:Sex Ratio
D004968:Estrogens, Non-Steroidal; D010636:Phenols; C080417:4-octylphenol