Liquid chromatography and electron-capture dissociation in Fourier transform ion cyclotron resonance mass spectrometry.
Liquid separation methods in combination with electrospray mass spectrometry as well as the recently introduced fragmentation method electron capture dissociation (ECD) have become powerful tools in proteomics research. This paper presents the results of the first successful attempts to combine liquid chromatography (LC) and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) with ECD in the analysis of a mixture of standard peptides and of a bovine serum albumin tryptic digest. A novel electron injection system provided conditions for ECD sufficient to yield extensive sequence information for the most abundant peptides in the mixtures on the time-scale of the chromatographic separation. The results suggest that LC/ECD-FTICRMS can be employed in the characterization of peptides in enzymatic digests of proteins or protein mixtures and identify and localize posttranslational modifications.
Palmblad
Magnus
M
Division of Ion Physics, The Angström Laboratory, Uppsala University, Box 534, SE-751 21, Sweden. magnus.palmblad@angstrom.uu.se
Tsybin
Youri O
YO
Ramström
Margareta
M
Bergquist
Jonas
J
Håkansson
Per
P
D002853:Chromatography, Liquid; D017357:Cyclotrons; D004583:Electrons; D005583:Fourier Analysis; D006868:Hydrolysis; D010455:Peptides / Q000737:chemistry*; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin
D010455:Peptides; D012710:Serum Albumin, Bovine; D014357:Trypsin