Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches.
Intermediate-resolution (7-9A) structures of large macromolecular complexes can be obtained by electron cryomicroscopy. This structural information, combined with bioinformatics data for the individual protein components or domains, can lead to a fold model for the entire complex. Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins.
Chiu
Wah
W
Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA. wah@bcm.tmc.edu
Baker
Matthew L
ML
Jiang
Wen
W
Zhou
Z Hong
ZH
D000595:Amino Acid Sequence; D020285:Cryoelectron Microscopy / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D046911:Macromolecular Substances; D008958:Models, Molecular; D017510:Protein Folding; D012984:Software
D046911:Macromolecular Substances