title: Detection of anti-ganglioside antibodies in Guillain-Barré syndrome and its variants by the agglutination assay.
abstract: Sera from 40 patients with Guillain-Barré syndrome (GBS), including the subtypes acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy (AMAN), acute motor and sensory axonal neuropathy (AMSAN), and Miller Fisher syndrome (MFS) were examined for the presence of anti-ganglioside antibodies using the ganglioside agglutination assay, and the enzyme-linked immunosorbent assay (ELISA). In the ELISA system, sera were tested for IgM and IgG antibodies to GM1, GM2, GD1a, GD1b, GT1b, and GQ1b gangliosides. Antibodies to gangliosides were detected in 21 (53%) of the GBS patients by agglutination assay and in 17 (43%) of the patients by ELISA. Some of the sera reacted with more than one ganglioside. Antibodies were not found in the control sera that were studied. The agglutination assay may be useful for rapid screening of GBS sera for antibodies to multiple gangliosides.
authors.affiliation: Department of Neurology and Neuroscience, Cornell University, 525 E. 68th St., Room LC 807, New York, NY 10021, USA. ara2004@med.cornell.edu
authors.forename: Armin
authors.initials: A
authors.lastname: Alaedini
authors.forename: Chiara
authors.initials: C
authors.lastname: Briani
authors.forename: Itzhak
authors.initials: I
authors.lastname: Wirguin
authors.forename: Gabriele
authors.initials: G
authors.lastname: Siciliano
authors.forename: Carla
authors.initials: C
authors.lastname: D'Avino
authors.forename: Norman
authors.initials: N
authors.lastname: Latov
mesh_terms: D000372:Agglutination Tests / Q000379:methods*; D000906:Antibodies / Q000097:blood*; D001369:Axons / Q000276:immunology* / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000276:immunology; D005678:G(M2) Ganglioside / Q000276:immunology; D005732:Gangliosides / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000097:blood* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010525:Peripheral Nerves / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D015203:Reproducibility of Results
chemical_list: D000906:Antibodies; D005732:Gangliosides; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1; C039129:GQ1b ganglioside