title: Assessment of human brain water content by cerebral bioelectrical impedance analysis: a new technique and its application to cerebral pathological conditions.
abstract: OBJECTIVE
  Total brain water content changes in several cerebral pathological conditions and the measurement of brain water content are important for the selection of appropriate therapeutic procedures. We present a quantitative, in vivo, bioelectrical impedance analysis (BIA) method and propose its use for the accurate assessment of brain water content among human subjects.
  
  
  METHODS
  Cerebral BIA is based on the conduction of an applied current in the brain parenchyma. Application of an excitatory current of 800 microA at 50 kHz, via two electrodes placed on the eyelids with the eyes closed, and detection of the voltage drop with two electrodes placed in the suboccipital region allow brain resistance and reactance to be measured. By means of an equation that considers cranial circumference and resistance, it is possible to quantify the total brain water content, expressed as the bioelectrical volume. Cerebral BIA was performed with a series of healthy volunteers (n = 100), for determination of average brain water content values. The method was then applied to 50 patients with brain tumors (n = 20), intracranial hemorrhage (n = 16), or hydrocephalus (n = 14), for assessment of changes in global brain water contents. Data were compared with those obtained for healthy volunteers.
  
  
  RESULTS
  Statistically significant differences (P < 0.001) were observed between the two groups. Mean brain water content values (expressed as bioelectrical volume values) were 38.2 +/- 3.9 cm2/Omega for healthy volunteers and 67.7 +/- 13.1 cm2/Omega for patients with cerebral pathological conditions. Statistically significant differences (P < 0.05) were also observed among patients with cerebral pathological conditions.
  
  
  CONCLUSIONS
  The results of this study suggest that BIA, applied to the cerebral parenchyma, is a valid method for the prediction of brain water contents under both normal and pathological conditions. However, further studies are needed to establish whether it is sensitive and reliable enough for future clinical applications.
authors.affiliation: Department of Neurosurgery, University of Messina, Messina, Italy. Giovanni.Grasso@unime.it
authors.forename: Giovanni
authors.initials: G
authors.lastname: Grasso
authors.forename: Concetta
authors.initials: C
authors.lastname: Alafaci
authors.forename: Marcello
authors.initials: M
authors.lastname: Passalacqua
authors.forename: Antonio
authors.initials: A
authors.lastname: Morabito
authors.forename: Michele
authors.initials: M
authors.lastname: Buemi
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Salpietro
authors.forename: Francesco
authors.initials: F
authors.lastname: Tomasello
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001834:Body Water / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000378:metabolism; D002543:Cerebral Hemorrhage / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005544:Forecasting; D006801:Humans; D006849:Hydrocephalus / Q000378:metabolism; D007223:Infant; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D012016:Reference Values