title: Reference values of lymphocyte subsets in the normal healthy adult Kuwaiti Arab population.
abstract: Fluorescent monoclonal antibody labelling followed by a lysed whole blood method and flow cytometry was used to determine the lymphocyte subpopulations in 127 (64 males and 63 females) normal healthy individuals in the adult (age 18-59 years) Kuwaiti population. Relative percentages and absolute values of CD2+, CD3+, CD19+, CD4+, CD8+, HLADR+, CD56+, CD45RO+, and CD45RA+ cells were determined. The reference ranges were CD2+, 73-92% (0.95-2.99 x 10(9) per l), CD3+, 64-85% (0.83-2.71 x 10(9) per l), CD19+, 6-22% (0.05-0.61 x 10(9) per l), CD4+, 34-54% (0.45-1.65 x 10(9) per l), CD8+, 20-42% (0.29-1.17 x 10(9) per l), HLADR+, 4-23% (0.02-0.62 x 10(9) per l), CD56+, 4-22% (0.06-0.58 x 10(9) per l), CD45RO+, 16-53% (0.26-1.42 x 10(9) per l) and CD45RA+, 35-72% (0.34-2.05 x 10(9) per l). The mean CD4/CD8 ratio was 1.50+/-0.35. CD3+ cells were positively correlated to both CD4+ and CD8+ cells (P<0.001), and CD4+ cells showed a significant positive correlation with CD8+ cells (P<0.001).
authors.affiliation: Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Kuwait University PO Box 31470, 90805, Sulaibikhat, Kuwait. kaaba@hsc.kuniv.edu.kw
authors.forename: Salim A
authors.initials: SA
authors.lastname: Kaaba
authors.forename: Suad
authors.initials: S
authors.lastname: Al Fadhli
authors.forename: Amal
authors.initials: A
authors.lastname: Khamis
mesh_terms: D000293:Adolescent; D000328:Adult; D018912:Arabs; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007730:Kuwait / Q000208:ethnology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000145:classification*; D008297:Male; D008875:Middle Aged; D012016:Reference Values