title: Structural organization of the estalpha3(1) gene in a Colombian strain of Culex quinquefasciatus differs from that in Cuba.
abstract: In Culex mosquitoes (Diptera: Culicidae), the most common mechanism for resistance to organophosphorus (OP) insecticides involves amplification of one or more esterases. Two esterase loci are often involved, with different allelic forms co-amplified. Estalpha3(1) is co-amplified with estbeta1 in a Colombian (COL) strain of Culex quinquefasciatus Say. These two alleles co-migrate on acrylamide gels, often leading to misscoring of the phenotype as elevation of a single estbeta enzyme. By sequencing COL genomic DNA, we determined the estalpha3(1) gene length is 1623 nucleotides. The open reading frame of estalpha3(1) encodes a 540 amino acid protein, as for estalpha2(1) in strain Pel RR from Sri Lanka. The intron/exon boundaries of estalpha3(1) are identical to those of estalpha2(1), suggesting that they are alleles of the same locus. The COL estalpha3(1) gene differs from estaalpha3(2) in strain MRES from Cuba, although they have equivalent electrophoretic mobility, showing that these two strains contain distinct resistance-associated amplicons. Twenty nucleotide differences were scored between the MRES partial 495 bp sequence and that in the COL strain, with two amino acid changes, demonstrating distinct estalpha enzymes. Our sequencing data show 95% identity between the three estalpha genes (each has six introns and seven exons) in OP-resistant Cx. quinquefasciatus. Amplified estalpha3(1) and estbeta1 are at least 10kb apart in temephos-selected COL and 2.7kb apart in Pel RR, whereas these non-amplified genes are only 1.7kb apart in the nonselected parental COL stock, as in Pel SS (susceptible Sri Lankan strain), demonstrating that this region of the genome is susceptible to expansion and contraction.
authors.affiliation: School of Biosciences, Cardiff University, Wales, UK.
authors.forename: D
authors.initials: D
authors.lastname: De Silva
authors.forename: J
authors.initials: J
authors.lastname: Hemingway
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003105:Colombia; D003462:Cuba; D003465:Culex / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D004950:Esterases / Q000235:genetics*; D005784:Gene Amplification; D017344:Genes, Insect; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds; D016133:Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D007306:Insecticides; D009943:Organophosphorus Compounds; D004950:Esterases