[Effects of blastocyst surface oligosaccharide LeY on secretion and expression of matrix metalloproteinase in vivo].
The effects of oligosaccharide LeY on expression and secretion of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase 1 (TIMP1) of embryos were investigated in cultured mouse blastocysts by zymography, immuno-dot blots and RT-PCR after blocking the oligosaccharide on embryo surface with specific monoclonal antibody AH6. The cultured blastocysts were treated with antibody AH6 for 3 h, then the antibody was removed and culture was continued, and the expression and secretion of MMP and TIMP1 were analyzed at 1.5 h, 3 h and 6 h. It was shown that, only 1.5 h after blocking, the expression of MMP2 gene and MMP9 gene in embryo was significantly declined, and expression of TIMP1 was slight increased, then secretions of MMP2, MMP9 were also declined at 3 h, but secretion of TIMP1 had no significant change during the period of observation. The results indicate that LeY may play a role of regulating the expression and secretion of MMP of mouse embryo at pre-implantation stage, by controlling the expression of MMP2 gene and MMP9 gene.
Ge
Chang-Hui
CH
Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027, China.
Kong
Ying
Y
Wang
Han
H
Zhu
Zheng-Mei
ZM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000494:pharmacology*; D001755:Blastocyst / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression / Q000187:drug effects*; D007983:Lewis Blood Group Antigens / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9