Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1.
The Pseudomonas aeruginosa PA1 strain, isolated from the water of oil production in Sergipe, Northeast Brazil, was evaluated as a potential rhamnolipid type of biosurfactant producer. The production of biosurfactants was investigated using different carbon sources (n-hexadecane, paraffin oil, glycerol, and babassu oil) and inoculum concentrations (0.0016-0.008 g/L). The best results were obtained with glycerol as the substrate and an initial cell concentration of 0.004 g/L. A C:N ratio of 22.8 led to the greatest production of rhamnolipids (1700 mg/L) and efficiency (1.18 g of rhamnolipid/g of dry wt).
Santa Anna
L M
LM
Centro de Pesquisas da Petrobras (Petrobras Research Center-CENPES), Rio de Janeiro, Brazil.
Sebastian
G V
GV
Pereira
N
N
Alves
T L
TL
Menezes
E P
EP
Freire
D M
DM
D019149:Bioreactors; D001938:Brazil; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D010578:Petroleum / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism*; D014871:Water Microbiology
D006017:Glycolipids; D010578:Petroleum; D013501:Surface-Active Agents; C418382:rhamnolipid; D002244:Carbon; D009584:Nitrogen; D005990:Glycerol