Lysine acetylsalicylate decreases proliferation and extracellular matrix gene expression rate in keloid fibroblasts in vitro.
BACKGROUND
In genetically predisposed individuals keloids are formed as benign collagenous tumors.


OBJECTIVE
The purpose of this study was to investigate whether the proliferation and matrix gene expression of keloid fibroblasts is differently influenced by the anti-inflammatory active drug lysine acetylsalicylate (LAS) when compared to normal skin fibroblasts in vitro.


METHODS
Normal skin and keloid fibroblasts derived from human donors were compared.


RESULTS
Excessive scarring and the formation of keloids are (at least in part) due to an overproduction of collagen types I and III. The results show a significant dose-dependent anti-proliferative effect of lysine acetylsalicylate. At the level of gene expression we observed a pronounced inhibitory effect of LAS on procollagen I and III mRNA synthesis, whereas matrix metalloproteinase 1 and tissue inhibitor of metalloproteinases 1 were not altered.


CONCLUSIONS
Further clinical studies are planned to evaluate these effects of a high-dose treatment of keloids with LAS.
Petri
Jean-Bernhard
JB
IBFB Institut für. Biomedizinische Forschung und Beratung, Karl-Heine-Str. 99, Leipzig, Germany. b.petri@ibfb.de
Haustein
Uwe-Frithjof
UF
D001241:Aspirin / Q000031:analogs & derivatives* / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D014945:Wound Healing
D012333:RNA, Messenger; D003094:Collagen; D008239:Lysine; D001241:Aspirin; C010395:acetylsalicylic acid lysinate