Localization of mLin-7 at nectin-based cell-cell junctions.
In C. elegans, lin-7 as well as lin-2/lin-10 is involved in the proper localization of the LET-23 receptor tyrosine kinase that regulates vulval induction. The mammalian homologue, mLin-7, forms a ternary complex with the mammalian homologues of LIN-2 and LIN-10 and localizes at cell-cell junctions in epithelial cells, but the mechanism of this localization of mLin-7 is unknown. Nectin is an immunoglobulin-like cell-cell adhesion molecule that is involved in organization of adherens and tight junctions in epithelial cells. Nectin is indirectly associated with the cadherin-catenin system and the actin cytoskeleton through afadin, an actin filament-binding protein. We showed here that mLin-7 localized at the nectin-based cell-cell junctions. This localization of mLin-7 required the interaction of nectin with afadin, but not the cadherin-catenin system or the actin cytoskeleton. mLin-7 did not directly interact with nectin or afadin. The results indicate that mLin-7 localizes at cell-cell junctions through the nectin-afadin system.
Yamamoto
Yasunori
Y
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan.
Mandai
Kenji
K
Okabe
Noriko
N
Hoshino
Takashi
T
Nakanishi
Hiroyuki
H
Takai
Yoshimi
Y
D008841:Actin Cytoskeleton / Q000502:physiology; D048868:Adaptor Proteins, Signal Transducing; D022005:Adherens Junctions / Q000737:chemistry*; D000818:Animals; D015820:Cadherins / Q000032:analysis / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000378:metabolism; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism; D016547:Kinesins; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D009218:Myosins; D000075983:Nectins; D011233:Precipitin Tests
D048868:Adaptor Proteins, Signal Transducing; C490657:Afdn protein, mouse; D015820:Cadherins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C119588:LIN-7 protein, mammalian; C114672:Lin7c protein, mouse; D008565:Membrane Proteins; D008840:Microfilament Proteins; D000075983:Nectins; C108835:afadin; D009218:Myosins; D016547:Kinesins