Production and analysis of neurospheres from acutely dissociated and postmortem CNS specimens.
Laywell
Eric D
ED
Departments of Neuroscience and Neurosurgery, McKnight Brain Institute, Shands Cancer Center, University of Florida, Gainesville, FL, USA.
Kukekov
Valery G
VG
Suslov
Oleg
O
Zheng
Tong
T
Steindler
Dennis A
DA
D000818:Animals; D000831:Animals, Newborn; D016380:Brain Tissue Transplantation / Q000295:instrumentation / Q000379:methods*; D002449:Cell Aggregation / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D003001:Cloning, Molecular / Q000379:methods; D003470:Culture Media; D015316:Genetic Therapy / Q000295:instrumentation / Q000379:methods*; D006133:Growth Substances; D006801:Humans; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008854:Microscopy, Electron; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D011180:Postmortem Changes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*
D003470:Culture Media; D006133:Growth Substances