[Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli].
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
Yu
Ji-Bin
JB
College of Life Sciences, Suzhou University, Suzhou 210006, China.
Ji
Ping
P
Zha
Xin-Min
XM
Shen
Wei-De
WD
Wu
Xiang-Fu
XF
D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012604:Scorpion Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000737:chemistry / Q000235:genetics*
C457223:BmK IT(3) scorpion toxin; D011994:Recombinant Proteins; D012604:Scorpion Venoms; D007544:Isopropyl Thiogalactoside