title: Cloning and nucleotide sequence analysis of the Streptococcus sobrinus gtfU gene that produces a highly branched water-soluble glucan.
abstract: Streptococcus sobrinus has four gtf genes, gtfI, gtfS, gtfT, and gtfU, on the chromosome. These genes correspond respectively to the enzymes GTF-I, GTF-S1, GTF-S2, and GTF-S3. An Escherichia coli MD66 clone that contained the S. sobrinus gtfU gene was characterized. Immunological properties showed that the protein produced by the E. coli MD66 clone was similar to S. sobrinus GTF-S1. Biological properties and a linkage analysis of the glucans by 13C NMR spectrometry revealed that the protein produced by the E. coli MD66 clone was GTF-S1.
authors.affiliation: Department of Oral Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Sinjuku-ku, Tokyo 162-8640, Japan. nhanada@nih.go.jp
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Hanada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Fukushima
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nomura
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Senpuku
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Hayakawa
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Mukasa
authors.forename: Teruaki
authors.initials: T
authors.lastname: Shiroza
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Abiko
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016986:Streptococcus sobrinus / Q000201:enzymology / Q000235:genetics*; D014162:Transfection
chemical_list: D005936:Glucans; D005964:Glucosyltransferases; C110463:glucosyltransferase C