Some aspects of matrix-assisted laser desorption/ionization analysis of hemoglobin from whole human blood.
Some aspects of the matrix-assisted laser desorption/ionization (MALDI) method in the analysis of hemoglobin from whole human blood are described. These aspects are: sensitivity of this method, fragmentation of hemoglobin ions, and influence of some impurities (sodium chloride NaCl, sodium sulfate Na2SO4, and potassium ferrocyanide K4Fe(CN)6) on the detection process. As a matrix, alpha-cyano-4-hydroxycinnamic acid (CCA) was used. The estimated maximum concentrations of tested salts for which no hemoglobin signal was observed were determined.
Bajuk
Anna
A
Institute of Physics, Maria Curie-Sklodowska University, 20-031 Lublin, Poland.
Michalak
Leszek
L
D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D012965:Sodium Chloride / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006454:Hemoglobins; D007202:Indicators and Reagents; D012965:Sodium Chloride