alpha-Tocopherol Inhibits IL-8 synthesis induced by thrombin and high glucose in endothelial cells.
It has been reported that alpha-tocopherol, an antioxidant agent, may play a role in preventing diabetic angiopathy. However, there is little evidence to show the effect of alpha-tocopherol on the production of pro-inflammatory cytokines in endothelial cells. Therefore, we examined the effect of alpha-tocopherol on the regulation of IL-8 synthesis induced by high glucose and/or thrombin in endothelial cells. Thrombin alone markedly increased the IL-8 release. Furthermore, high glucose levels and thrombin combined had additive effects on IL-8 synthesis, and alpha-tocopherol diminished their effect; alpha-tocopherol also inhibited the phosphorylation of IkappaB-alpha induced by high glucose levels and/or thrombin. Our results suggest that the administration of alpha-tocopherol to diabetic patients may have a beneficial effect for the prevention of diabetic vascular complications by the inhibition of IL-8 synthesis from endothelial cells.
Nobata
Y
Y
Toyama Medical and Pharmaceutical University, Faculty of Medicine, First Department of Internal Medicine, 2630 Sugitani, Toyama-shi, Japan.
Urakaze
M
M
Temaru
R
R
Sato
A
A
Nakamura
N
N
Yamazaki
K
K
Kishida
M
M
Takata
M
M
Kobayashi
M
M
D001011:Aorta; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D020672:I-kappa B Proteins; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D000072000:NF-KappaB Inhibitor alpha; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D013917:Thrombin / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D016209:Interleukin-8; C000606204:NFKBIA protein, human; D012333:RNA, Messenger; D000072000:NF-KappaB Inhibitor alpha; D013917:Thrombin; D024502:alpha-Tocopherol; D005947:Glucose