[In vitro transcription of inducible nod gene nodA].
We observed in vitro transcription of nodA, an inducible nod gene. Primer extension analysis of the transcripts showed there always existed a transcription product identical to that of its in vivo transcription, when 10 mumol/L naringening and NodD protein was added in the in vitro transcription system. Induced in vitro transcription of nodA gene suggests that in its in vivo transcription there may also exist a kind of physical interaction among NodD protein, inducer naringening and promoter of nodA gene.
Zhang
Lei
L
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China.
Hong
Guo-Fan
GF
D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D008969:Molecular Sequence Data; D016961:Rhizobium leguminosarum / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C060773:NodD protein, Bacteria; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales