Physical and genetic map of the Bacteroides fragilis YCH46 chromosome.
The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.
Kuwahara
Tomomi
T
Department of Bacteriology, School of Medicine, The University of Tokushima, Tokushima 770-8503, Kuramoto-cho, Japan.
Sarker
Mahfuzur Rahman
MR
Ugai
Hideyo
H
Akimoto
Shigeru
S
Shaheduzzaman
Syed Mohammed
SM
Nakayama
Haruyuki
H
Miki
Tsuyoshi
T
Ohnishi
Yoshinari
Y
D001441:Bacteroides fragilis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D020161:Physical Chromosome Mapping
C078382:endodeoxyribonuclease AscI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases