title: [Experimental study on subcutaneous bone formation by marrow stromal osteoblast-cancellous bone matrix compound artificial bone].
abstract: OBJECTIVE
  To investigate the feasibility of using marrow stromal osteoblast (MSO) as bone derived cell and using cancellous bone matrix (CBM) as scaffold for bone tissue engineering, the subcutaneous osteogenesis of MSO-CBM compound artificial bone (MCCAB) was observed in the experiment.
  
  
  METHODS
  The marrow stromal cells of adult New Zealand rabbits cultivated and induced in vitro were used to form MCCAB by mixing, seeding and solidifying methods assisted by alginate. The MCCABs were auto-transplanted subcutaneously into the rabbits for 4 to 8 weeks. The alginate-cancellous bone matrix composites or the cancellous bone matrix alone were implanted as control. The effectiveness of bone formation was assessed by means of roentgenography, histology and computerized histomorphometry.
  
  
  RESULTS
  The osteogenesis of MCCABs was better than that of the alginate-cancellous bone matrix composites and of the cancellous bone matrixes. In the MCCABs, both intramembranous and cartilaginous osteogeneses were seen but the former was obvious. In the control, only slight cartilaginous osteogeneses were seen.
  
  
  CONCLUSIONS
  The osteogeneses of the MCCABs constructed by using tissue engineering method were obvious when transplanted subcutaneously. The MSO and CBM can be used as good bone-derived cell and scaffold material respectively for tissue-engineered bone construction.
authors.affiliation: Laboratory of Tissue Engineering, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Tao
authors.forename: Tianqiu
authors.initials: T
authors.lastname: Mao
authors.forename: Fulin
authors.initials: F
authors.lastname: Chen
authors.forename: Weidong
authors.initials: W
authors.lastname: Yang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Gu
authors.forename: Shujun
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D001857:Bone Matrix / Q000637:transplantation*; D016025:Bone Transplantation / Q000379:methods*; D008297:Male; D010006:Osteoblasts / Q000637:transplantation*; D010012:Osteogenesis; D011817:Rabbits; D023822:Tissue Engineering