title: Macrolide and tetracycline resistance and molecular relationships of clinical strains of Streptococcus agalactiae.
abstract: Mechanisms for tetracycline and macrolide resistance in 54 isolates of erythromycin-resistant Streptococcus agalactiae were analyzed by PCR. The erm(B), erm(A), and mef(A) genes, either alone or in combination, were detected in all the erythromycin-resistant isolates. The tet(M) and tet(O) genes were responsible for tetracycline resistance. Random amplification of polymorphic DNA indicated different clonal origins of the isolates.
authors.affiliation: Department of Clinical Microbiology, Hospital Universitario San Carlos, 28040 Madrid, Spain. eculebras@cbm.uam.es
authors.forename: Esther
authors.initials: E
authors.lastname: Culebras
authors.forename: Iciar
authors.initials: I
authors.lastname: Rodriguez-Avial
authors.forename: Carmen
authors.initials: C
authors.lastname: Betriu
authors.forename: Montserrat
authors.initials: M
authors.lastname: Redondo
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Picazo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000187:drug effects* / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology*; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004917:Erythromycin; D013752:Tetracycline