[Synthesis and expression of 42 kD C-terminal region of the major merozoite surface protein (MSP1 - 42) of P. falciparum 3D7 strain in pichia pastoris].
OBJECTIVE
Production of 3D7/MSP1 - 42 recombinant protein with correct conformation in Pichia pastoris for vaccine efficiency assay.


METHODS
Asymmetric PCR-based method was utilized to synthesize the 1 202 bp 3D7/msp1 - 42 gene. The expressing plasmid containing the synthetic gene was introduced into Pichia pastoris by electroporation. The secreted product was detected by Western Blot.


RESULTS
The redesigned entire 3D7/msp1 - 42 gene was generated with error-free, and expressed to produce 42 kD recombinant protein in secreted form. Conformational monoclonal antibody specific for MSP1 C-terminal can interact with the recombinant protein.


CONCLUSIONS
The redesigned 3D7/msp1 - 42 gene was expressed in P. pastoris with full length of recombinant protein which resembled most likely to the native protein.
Zhang
Dongmei
D
Institute of Medical Biotechnology & Molecular Genetics of Second Military Medical University, Shanghai 200433 China.
Pan
Weiqing
W
Lu
Deru
D
Jiang
Liping
L
D000818:Animals; D018274:Electroporation / Q000379:methods; D020066:Merozoite Surface Protein 1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D010843:Pichia / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic
D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins