Phytosecretion of enteropathogenic Escherichia coli pilin subunit A in transgenic tobacco and its suitability for early life vaccinology.
A plant expression cassette system was engineered to efficiently target proteins to the default secretory pathway, allowing the expression of DNA inserts in three frames as fusion proteins containing a synthetic tobacco calreticulin cleavable signal peptide sequence, with the advantage of producing the recombinant proteins by phytosecretion. As one approach to develop a vaccine to enteropathogenic Escherichia coli (EPEC) infection, the oral immunogenicity of phytosecreted BfpA, the structural subunit A of the bundle-forming pilus, expressed at high levels (7.7% of soluble protein) in transgenic tobacco tissues, was demonstrated in BALB/c mice by the induction and detection of fecal anti-BfpA antibodies.
Vieira da Silva
Jeferson
J
Plant Biotechnology Section, Laboratory of Biotechnology, Center for Biosciences and Biotechnology, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego 2000, Horto, Campos dos Goytacazes, RJ CEP 28015-620, Brazil.
Garcia
Ana Beatriz
AB
Vieira Flores
Victor Martin Quintana
VM
Sousa de Macedo
Zila
Z
Medina-Acosta
Enrique
E
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines / Q000276:immunology*; D036781:Fimbriae Proteins; D005822:Genetic Vectors; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000276:immunology* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; C104901:BfpA protein, E coli; D029968:Escherichia coli Proteins; D022361:Escherichia coli Vaccines; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D036781:Fimbriae Proteins