Effect of rhynchophylline on apoptosis induced by dopamine in NT2 cells.
OBJECTIVE
To study the effect of rhynchophylline (Rhy) on the apoptosis induced by dopamine (DA) in NT2 cell line.


METHODS
Release of lactate dehydrogenase (LDH) from cultured cells was determined by enzyme-linked immunosorbant assay; apoptotic NT2 neurons by terminal de oxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling method; DNA fragmentation by agarose gel electrophoresis; Bcl-2 protein expression by Western blotting.


RESULTS
DA 100 - 500 micromol/L increased release of LDH, number of apoptotic NT 2 neurons, and DNA fragmentation (P < 0.01). NT2 cells survival rate was decreased. Rhy 5 and 50 micromol/L reduced the percentage of apoptosis after treated with DA 100 micromol/L for 6 h in both with and without bcl-2 gene neurons (P < 0.05, P < 0.01, respectively). The expression of Bcl-2 protein was not affected by Rhy.


CONCLUSIONS
Rhy protected NT2 neurons from the damage induced by DA.
Shi
Jing-Shan
JS
Department of Pharmacology, Zunyi Medical College, Zunyi 563003, China.jsshi@zmc.gz.cn
Kenneth
Haglid G
HG
D000470:Alkaloids / Q000494:pharmacology*; D017209:Apoptosis; D004298:Dopamine / Q000494:pharmacology*; D004347:Drug Interactions; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D026121:Indole Alkaloids; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D000078183:Oxindoles; D018243:Teratocarcinoma / Q000473:pathology; D014162:Transfection; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D026121:Indole Alkaloids; D018696:Neuroprotective Agents; D000078183:Oxindoles; C052714:rhyncophylline; D004298:Dopamine