[Responses induced by dopamine in   Xenopus laevis  oocytes injected with mRNA from heat injured rat striatum].
Poly(A)(+) mRNA isolated from the striatum of normal rats and heat injured rats by RNAgents Total RNA Isolation System and PolyTract mRNA RNAgents Isolation Systems was microinjected into   Xenopus laevis   oocytes to express functional neurotransmitter receptors. Membrane currents were recorded by the conventional patch clamp technique for measuring the relative amounts of mRNA encoding dopamine receptor in order to study the change in dopamine receptors in heat injured rat striatum. There was no change in resting membrane potentials of the oocytes before and after mRNA injection. Dopamine induced membrane currents were shown to be mediated through Cl(  ). The oocytes injected with mRNA from heat injured rat striatum were less sensitive to dopamine than those injected with mRNA from normal rats (  P<0.05). The above result showed that heat injury affected gene expression and signal transduction of dopamine receptors in rat striatum.
Zhao
Y Q
YQ
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China. xingch@nic.bmi.ac.cn
Zhang
B L
BL
Wang
L M
LM
Xing
C
C
Li
M
M
Fan
M
M
D000818:Animals; D001833:Body Temperature Regulation; D003342:Corpus Striatum / Q000502:physiology*; D004594:Electrophysiology; D005260:Female; D006358:Hot Temperature; D008297:Male; D008564:Membrane Potentials; D009865:Oocytes / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011954:Receptors, Dopamine / Q000378:metabolism*; D014982:Xenopus laevis
D012333:RNA, Messenger; D011954:Receptors, Dopamine