Cloning and characterization of a novel isoform of calpastatin in human adult testis.
OBJECTIVE
To clone a new gene related to human spermatogenesis.


METHODS
cDNA probes of embryo and adult testis were used to hybridize the cDNA microarray of adult testis, and the clones of differential hybridization were sequenced and analyzed.


RESULTS
A novel isoform of calpastatin exclusively and highly expressed in human adult testis was found.


CONCLUSIONS
A novel isoform of calpastatin expresses in human testis and it is related to spermatogenesis.
Zhu
Hu
H
Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 210029  China. shajh@njmu.edu.cn
Zhou
Zuo-Min
ZM
Li
Jian-Min
JM
Zhu
Hui
H
Cheng
Li-Jun
LJ
Shan
Yu-Xi
YX
Yin
Lan-Lan
LL
Sha
Jia-Hao
JH
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002154:Calpain / Q000037:antagonists & inhibitors*; D003001:Cloning, Molecular; D005333:Fetus; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D020033:Protein Isoforms; C033668:calpastatin; D002154:Calpain