[Glutathione-related enzymes activity during vitrectomy. Effect of BSS Plus(R) in retinal tissue].
OBJECTIVE
To determine changes in the retinal activity of two enzymes related to the glutathione metabolism (Glutathione synthetase -GSHS- and glutathione reductase -GSSGR-) after vitrectomy using BSS Plus(R).


METHODS
Forty-five pigmented rabbits were distributed in 6 groups: group I (GSHS control in retinal tissue); Group II (analysis of GSHS at 3 hours post-vitrectomy); Group III (determination of GSHS at 48 hours after vitrectomy); Group IV (GSSGR control in retinal tissue); Group V (measurement of GSSGR at 3 hours post-surgery) and Group VI (study of GSSGR at 48 hours post-vitrectomy). Statistical analysis was done by a parametric test (ANOVA of single factor) (p<0.05).


RESULTS
The activity of these enzymes was: Group I (n=5, basal GSHS). 2785,63 D.S. 419,51 U/g; Group II (n=4, GSHS at 3 hours). 6053,50 D.S. 2788,84 U/g; Group III (n=4, GSHS at 48 hours). 7424,30 D.S. 997,47 U/g; Group IV (n=10, basal GSSGR). 150,86 D.S. 24,40 mU/mL; Group V (n=7, GSSGR at 3 hours). 212,03 D.S. 53,30 mU/mL; Group VI (n=8, GSSGR at 48 hours). 210,84 D.S. 46,03 mU/mL.


CONCLUSIONS
At retinal tissue, BSS Plus(R) intraocular irrigating solution increases GSHS activity without modifications of GSSGR levels. It seems to be related to the <<novo>> synthesis of glutathione.
Marcos
M A
MA
Unidad de Retina, Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid.
Cordero
Y
Y
Manzanas
L
L
del Nozal
M J
MJ
Pastor
J C
JC
D000818:Animals; D001639:Bicarbonates; D004573:Electrolytes; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione; D005980:Glutathione Reductase / Q000378:metabolism*; D005981:Glutathione Synthase / Q000378:metabolism*; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D011817:Rabbits; D012160:Retina / Q000187:drug effects* / Q000201:enzymology; D012965:Sodium Chloride; D007507:Therapeutic Irrigation; D014821:Vitrectomy
C103640:BBS Plus; D001639:Bicarbonates; D004573:Electrolytes; D009883:Ophthalmic Solutions; D012965:Sodium Chloride; D005980:Glutathione Reductase; D005981:Glutathione Synthase; D005978:Glutathione