[Comparison of inhibitory effects of three natriuretic peptides on the proliferation of pulmonary artery smooth muscle cells of rats].
The inhibitory effects of atrial natriuretic peptide (ANP), C-type natriuretic peptide (CNP) and vasonatrin peptide (VNP) on the proliferation of pulmonary artery smooth muscle cells (PASMCs) were compared in rats. Total protein and OD value of MTT were detected in cultured rat PASMCs to examine the influence of the three peptides on PASMC proliforation induced by phorbol 12-myristate 13-acetate (PMA). The results show that PMA (10(  9)  10(  7) mol/L) increased and VNP (10(  8)  10(  6) mol/L), ANP and CNP (10(  7)  10(  6) mol/L) decreased the total protein and OD value in a dose-dependent manner (P<0.05). The data above suggest that PMA stimulates the proliferation of PASMCs while the three peptides inhibit the proliferation induced by PMA. Of the three peptides VNP has the strongest inhibitory effect.
Dong
M Q
MQ
Department of Physiology, Fourth Military Medical University, Xi an 710032, China. DMQ212@253.net
Zhu
M Z
MZ
Yu
J
J
Shang
L J
LJ
Feng
H S
HS
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D020098:Natriuretic Peptide, C-Type / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
D020098:Natriuretic Peptide, C-Type; C068986:ventricular natriuretic peptide, eel; D009320:Atrial Natriuretic Factor