Significant increase of colonic mutated crypts in ulcerative colitis correlatively with duration of illness.
Mild periodic acid-Schiff (mPAS) staining can discriminate non-O-acetylated(mPAS positive) from O-acetylated (mPAS negative) epithelial sialoglycoproteins in human colonic mucosa, giving three haplotypes of expression of a single polymorphic autosomal gene (oat). Increase in mPAS-positive crypts in heterozygotes is an indication of mutations, and wholly mPAS-positive (stem cell mutated) crypts and clusters of two or more mPAS-positive crypts in heterozygotes of ulcerative colitis (P < 0.0001) were found to be increased significantly, compared with controls. The observed correlation with ulcerative colitis duration (r = 0.892 and 0.853, respectively) supports a chronic inflammation-carcinoma sequence.
Okayasu
Isao
I
Department of Pathology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan. isaokaya@med.kitasato-u.ac.jp
Hana
Kiyomi
K
Yoshida
Tsutomu
T
Mikami
Tetuo
T
Kanno
Jun
J
Fujiwara
Mutsunori
M
D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D010503:Periodic Acid-Schiff Reaction; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors