title: [Effect of intrauterine hepatitis B virus infection on hepatitis B vaccine inoculation in newborns].
abstract: OBJECTIVE
  To study the effect and the mechanism of peripheral blood nuclear cells (PBMC) invaded by hepatitis B virus (HBV) on the artificial immunization in newborns.
  
  
  METHODS
  Fifty-two newborns, whose mothers were hepatitis B surface antigen (HBsAg) positive, were immunized with hepatitis B immunoglobulin and hepatitis B vaccine (HBVac), and then followed for 7 months. The newborns' serum and PBMC HBV DNA was detected by nested-PCR, hepatitis B surface antibody (HBsAb) was tested with solid phase radioimmunoassay. PBMC from newborn were incubated with PHA and HBsAg. The supernatant interleukin 2 (IL-2) level was measured by enzyme linked immununosorbent assay (ELISA).
  
  
  RESULTS
  The rate of vaccination failure was higher in the infants with PBMC HBV DNA positive than those with negative (P < 0.05). The supernatant IL-2 level was lower in the former than that in the latter and the control (P < 0.05). The level of IL-2 in the immunization failure newborns was lower than that in the vaccination success and the control (P < 0.05).
  
  
  CONCLUSIONS
  The intrauterine PBMC HBV invasion is one of the important causes of vaccination failure in the newborns. PBMC IL-2 autocrine down regulation is closely related to HBV invasion, that may lead to the failure of HBVac inoculation in the newborns.
authors.affiliation: Department of Obstetrics and Gynecology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jinlai
authors.initials: J
authors.lastname: Meng
authors.forename: Yafei
authors.initials: Y
authors.lastname: Yue
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhang
mesh_terms: D005260:Female; D006509:Hepatitis B / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control* / Q000635:transmission; D017325:Hepatitis B Vaccines / Q000627:therapeutic use*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008297:Male; D014599:Uterus / Q000821:virology; D014611:Vaccination
chemical_list: D017325:Hepatitis B Vaccines; D007376:Interleukin-2