title: In vivo characterization of the molecular-genetic changes in gastric mucosa during the development of acute gastritis and stress ulceration.
abstract: BACKGROUND
  Trauma patients are at risk for the development of stress ulceration. Stress ulceration should be associated with increased heat-shock gene (iHSP70) and an inhibition of the trefoil peptide, spasmolytic polypeptide (SP), and mucin (MUC5AC) gene expressions.
  
  
  METHODS
  Male Sprague-Dawley rats (10 weeks old) were restrained for 0-, 4-, 8-, 12-, and 24-hour periods of time. Gastric ulcers were graded using a one- to three-point scoring system. The level of mucosal gene expression was determined at each time point for three genes: iHSP70, SP, and MUC5AC.
  
  
  RESULTS
  Gastric ulceration developed in direct proportion to the duration of restraint. Gastric ulceration was preceded by increased iHSP70 and decreased SP and MUC5AC gene expressions.
  
  
  CONCLUSIONS
  Restraint-induced gastric ulceration was preceded by an up-regulation of iHSP70 and a down-regulation of SP and MUC5AC gene expressions.
authors.affiliation: Department of EMS/Trauma Surgery, Hartford Hospital, Hartford 06102-5037, USA.
authors.forename: Darryl
authors.initials: D
authors.lastname: Choo
authors.forename: Khalid
authors.initials: K
authors.lastname: Khwaja
authors.forename: Kenneth
authors.initials: K
authors.lastname: Nori
authors.forename: Michael
authors.initials: M
authors.lastname: Rewinski
authors.forename: Richard
authors.initials: R
authors.lastname: Zeff
authors.forename: George
authors.initials: G
authors.lastname: Perdrizet
mesh_terms: D000208:Acute Disease; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006133:Growth Substances / Q000096:biosynthesis*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D055271:Mucin 5AC; D009077:Mucins / Q000096:biosynthesis*; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014456:Ulcer / Q000235:genetics* / Q000378:metabolism
chemical_list: D006133:Growth Substances; D018840:HSP70 Heat-Shock Proteins; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3