title: Expression of a membrane-anchored glycoprotein, the influenza virus hemagglutinin, by dicistronic replicons derived from the poliovirus genome.
abstract: Mono- and dicistronic poliovirus replicons were constructed to express the influenza virus hemagglutinin, retaining its signal peptide and transmembrane region. Picornavirus genomes do not normally encode glycoproteins, and only the dicistronic replicon, in which the foreign and poliovirus sequences were separated by the encephalomyocarditis virus internal ribosomal entry site, replicated and expressed glycosylated hemagglutinin.
authors.affiliation: Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 75724 Paris Cédex 15, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Vignuzzi
authors.forename: Sylvie
authors.initials: S
authors.lastname: Gerbaud
authors.forename: Sylvie
authors.initials: S
authors.lastname: van der Werf
authors.forename: Nicolas
authors.initials: N
authors.lastname: Escriou
mesh_terms: D003001:Cloning, Molecular; D005822:Genetic Vectors; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009975:Orthomyxoviridae / Q000276:immunology / Q000378:metabolism*; D017955:Poliovirus / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D012093:Replicon; D014162:Transfection
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins