Differential expressions of Fas and Fas ligand in human placenta.
To investigate the expressions of Fas and Fas ligand (FasL) in human placenta, we studied the expressions of Fas and FasL in placenta with RT-PCR, immunoblotting and immunostaining. We observed amplified products of Fas and FasL transcripts, the band of Fas (52 kDa) and multiple bands of FasL (42-52 kDa) in placenta. Fas and FasL localized mainly on fetal vessels and on syncytiotrophoblasts respectively. The differential distribution of Fas and FasL in human placenta may reflect intrinsic expressions of them by trophoblasts during differentiation. The increased expression of Fas in trophoblasts may promote apoptosis of placenta in pathologic condition such as preeclampsia.
Roh
Cheong-Rae
CR
Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. crroh@smc.samsung.co.kr
Lee
Jeong-Won
JW
Kang
Byung-Hee
BH
Yang
Soon-Ha
SH
Kim
Byoung-Gie
BG
Bae
Duk-Soo
DS
Kim
Jong-Hwa
JH
Lee
Je-Ho
JH
D053222:Fas Ligand Protein; D015870:Gene Expression; D020869:Gene Expression Profiling; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D010920:Placenta / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism; D019014:fas Receptor / Q000096:biosynthesis / Q000235:genetics*
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D019014:fas Receptor