title: Regulation of human Fc epsilon RI alpha-chain gene expression by multiple transcription factors.
abstract: Transcriptional regulation of the gene-encoding human Fc epsilon RI alpha-chain was analyzed in detail. EMSA revealed that either YY1 or PU.1 bound to the region close to that recognized by Elf-1. The alpha-chain promoter activity was up-regulated approximately 2-fold by exogenously expressed YY1 or PU.1 and approximately 7-fold by GATA-1, respectively, in KU812 cells. In contrast, coexpression of GATA-1 with either of PU.1 or YY1 dramatically activated the promoter approximately 41- or approximately 27-fold, respectively. Especially synergic activation by GATA-1 and PU.1 was surprising, because these transcription factors are known to inhibit the respective transactivating activities of each other. These up-regulating effects of PU.1 and YY1 with GATA-1 were inhibited by overexpression of Elf-1, indicating that Elf-1 serves as a repressor for the alpha-chain gene expression. Transcriptional regulation of the alpha-chain gene through four transcriptional factors is discussed.
authors.affiliation: Allergy (Atopy) Research Center, Juntendo University School of Medicine, Tokyo, Japan. chinishi@med.juntendo.ac.jp
authors.forename: Chiharu
authors.initials: C
authors.lastname: Nishiyama
authors.forename: Masanari
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishiyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Takahashi
authors.forename: Yushiro
authors.initials: Y
authors.lastname: Akizawa
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Yokota
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
mesh_terms: D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000378:metabolism; D017455:Receptors, IgE / Q000737:chemistry / Q000235:genetics*; D012097:Repressor Proteins / Q000378:metabolism; D020218:Response Elements; D015534:Trans-Activators / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D015533:Transcriptional Activation; D050986:YY1 Transcription Factor; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D000906:Antibodies; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D011518:Proto-Oncogene Proteins; D017455:Receptors, IgE; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050986:YY1 Transcription Factor; D000099232:Proto-Oncogene Protein Spi-1; C494688:GATA1 protein, human; C494698:YY1 protein, human