title: Subunit composition and function of GABAA receptors of rat spermatozoa.
abstract: GABA triggers mammalian sperm acrosome reaction (AR). Here, evidence is presented, showing that rat spermatozoa contain GABAA receptors, composed of alpha5, beta1 and beta3 subunits. The effects of GABAA receptor agonist and antagonist on the induction of AR in rat spermatozoa were assessed using the chlortetracycline assay. Muscimol, a GABAA receptor agonist, triggered AR; whereas bicuculline, a GABAA receptor antagonist and picrotoxin, a GABAA receptor/Cl- channel blocker, inhibited the ability of GABA or progesterone to induce AR. In conclusion, GABAA receptors appear to mediate the action of progesterone in inducing AR in rat spermatozoa.
authors.affiliation: Laboratory of Molecular Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, People's Republic of China.
authors.forename: Jing Hua
authors.initials: JH
authors.lastname: Hu
authors.forename: Xiao Bing
authors.initials: XB
authors.lastname: He
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Yuan Chang
authors.initials: YC
authors.lastname: Yan
authors.forename: S S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D000818:Animals; D001483:Base Sequence; D001640:Bicuculline / Q000494:pharmacology; D017931:DNA Primers; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D021122:Protein Subunits; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000502:physiology*; D014158:Transcription, Genetic; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology / Q000502:physiology
chemical_list: D001640:Bicuculline; D017931:DNA Primers; D009118:Muscimol; D010852:Picrotoxin; D021122:Protein Subunits; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid