Beta-arrestin is involved in the desensitization but not in the internalization of the somatostatin receptor 2A expressed in CHO cells.
The interaction of beta-arrestin-1 with the somatostatin receptor type 2A (sst2A) was monitored using both biochemical and confocal imaging approaches. We show that, using transient transfection of either beta-arrestin-1 or its dominant negative Delta-arrestin-1 in CHO cells stably transfected with the sst2A, beta-arrestin-1 is colocalized with the receptor in endosomal vesicles after somatostatin-induced sequestration. However, this interaction leads to a role of beta-arrestin-1 in the desensitization of the sst2A rather than in the internalization process of the receptor-ligand complex.
Brasselet
Sabrina
S
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, UMR 6097, 660 route des Lucioles, 06560, Valbonne, France.
Guillen
Stéphanie
S
Vincent
Jean-Pierre
JP
Mazella
Jean
J
D000818:Animals; D019390:Arrestins / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active; D016466:CHO Cells; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D007150:Immunohistochemistry; D007700:Kinetics; D008024:Ligands; D018613:Microscopy, Confocal; D017481:Receptors, Somatostatin / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology; D014162:Transfection; D000071557:beta-Arrestins
D019390:Arrestins; D008024:Ligands; D017481:Receptors, Somatostatin; D011994:Recombinant Proteins; D000071557:beta-Arrestins; D013004:Somatostatin; C093733:somatostatin receptor 2; D000242:Cyclic AMP