The role of histamine H(1) receptors in late-phase reaction of allergic conjunctivitis.
The role of histamine H(1) receptors in the late-phase reaction of allergic conjunctivitis was studied using histamine H(1) receptor-deficient mice. To clarify the eosinophil infiltration, which is a reliable indicator of late-phase reaction, eosinophil peroxidase activity in the conjunctiva was measured. Mice were actively immunized with ovalbumin, and conjunctivitis was induced by topical instillation of ovalbumin. A significantly high eosinophil peroxidase level in the conjunctiva was observed in sensitized wild-type mice, whereas sensitized histamine H(1) receptor-deficient mice showed no significant increase in the conjunctival eosinophil peroxidase level. In addition, the elevation of eosinophil peroxidase level observed in sensitized wild-type mice was significantly antagonized by pretreatment with anti-P-selectin antibody. From these findings, it was concluded that eosinophil infiltration into the conjunctival tissue in late-phase reaction of allergic conjunctivitis is mediated by P-selectin stored in endothelial cells via histamine H(1) receptors.
Izushi
Keiji
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan.
Nakahara
Hiroto
H
Tai
Nobuyuki
N
Mio
Mitsunobu
M
Watanabe
Takeshi
T
Kamei
Chiaki
C
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D003228:Conjunctiva / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D003233:Conjunctivitis, Allergic / Q000201:enzymology / Q000276:immunology*; D047209:Eosinophil Peroxidase; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019007:P-Selectin / Q000276:immunology; D010544:Peroxidases / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000235:genetics / Q000502:physiology*; D013997:Time Factors
D000906:Antibodies; D019007:P-Selectin; D011969:Receptors, Histamine H1; D047209:Eosinophil Peroxidase; D010544:Peroxidases