[Effects of recombinant human leukemia inhibitor on the preimplantation mouse embryo development in vitro].
OBJECTIVE
To assess the effect of recombinant human leukemia inhibitory factor (rhLIF) on mouse embryo development in vitro.


METHODS
Mice were randomly divided to three groups, one in vivo control (group I) and two in vitro (group II and III). Mice were sacrificed at 116 - 120 hours (group I) and 44 - 48 hours (group II and III) subsequent human chorionic gonadotropin (hCG) injection. Two-cell embryos (group II and III) and blastocysts (group I) were obtained. Embryos in group II were cocultured with human tubal fluid (HTF) + 10% human serum and in group III with HTF + 10% human serum + rhLIF (1 000 U/ml). The number of embryo in different stage was recorded and compared.


RESULTS
Embryo in four, eight-cell and morula was noted in group II and III, 87.7% versus 91.2% and 75.0% versus 85.4% respectively. There was no significant difference. However, further embryo development to the blastocyst, expanded blastocyst, and hatching blastocyst in group II (48.1%, 32.1% and 18.4%) was lower than that in group III (82.3%, 59.7% and 36.3%). There was no difference between blastocyst in group I and group III (86.0% vs 82.3%).


CONCLUSIONS
RhLIF does not provide obvious stimulation in early mouse embryo, however, rhLIF can promote the growth, differentiation, and hatching of preimplantion blastocyst.
Wang
Guoyun
G
Department of Obstetrics, Shandong Maternal and Child Health Hospital, Jinan 250014, China.
Deng
Xiaohui
X
Zhang
Huiqin
H
Yu
Hongling
H
Jiang
Sen
S
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins