Improved polygalacturonase production from Bacillus sp. MG-cp-2 under submerged (SmF) and solid state (SSF) fermentation.
OBJECTIVE
To investigate the effect of amino acids, vitamins and surfactants on polygalacturonase production from Bacillus sp. MG-cp-2 under submerged (SmF) and solid state fermentation (SSF).


RESULTS
Bacillus sp. MG-cp-2 was isolated from the outer covering of the seeds of Celastrus paniculatus. Out of the various surfactants, amino acids and vitamins, Tween-60, DL-serine and folic acid maximally enhanced polygalacturonase production by 2.7-fold (240.0 U x ml(-1)), 4.0-fold (360.0 U x ml(-1)) and 3.8-fold (342.0 U x ml(-1)) respectively, under submerged fermentation (SmF). In solid state fermentation (SSF), Tween-80, pyridoxine and DL-ornithine monohydrochloride induced highest enzyme production up to 1.73-fold (6956.5 U x g(-1)), 5.3-fold (21224.4 U x g(-1)) and 5.74-fold (23076.9 U x g(-1)), respectively.


CONCLUSIONS
Amino acids and their analogues, vitamins and surfactants effect significantly polygalacturonase production by Bacillus sp. MG-cp-2 when grown under submerged (SmF) and solid state fermentation (SSF) conditions.


CONCLUSIONS
The study provides useful information about regulation of polygalacturonase biosynthesis in Bacillus sp. MG-cp-2, which appears to be an interplay of nutritional and physical factors. Alkaline polygalacturonase from Bacillus sp. MG-cp-2 will be extremely useful in the treatment of alkaline pectic waste waters from vegetable and fruit processing industries and in degumming of bast fibres.
Kapoor
M
M
Department of Microbiology, University of Delhi South Campus, New Delhi, India.
Kuhad
R C
RC
D000596:Amino Acids / Q000494:pharmacology; D001407:Bacillus / Q000201:enzymology*; D019149:Bioreactors; D005285:Fermentation; D011096:Polygalacturonase / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
D000596:Amino Acids; D013501:Surface-Active Agents; D014815:Vitamins; D011096:Polygalacturonase