An N-terminal truncated form of Orp150 is a cytoplasmic ligand for the anti-proliferative mushroom Agaricus bisporus lectin and is required for nuclear localization sequence-dependent nuclear protein import.
Nuclear localization sequence-dependent nuclear protein import is essential for maintaining cell function and can be selectively blocked in epithelial cells by mushroom (Agaricus bisporus) lectin. Here we report that a major intracellular ligand for this lectin is an N-terminally truncated form of oxygen-regulated protein 150 (Orp150), which lacks the endoplasmic reticulum translocation signal peptide of full-length Orp150. This cytoplasmic form of Orp150 expresses the lectin carbohydrate ligand (sialyl-2,3-galactosyl-beta1,3-N-acetylgalactosamine-alpha) and is shown to be essential for nuclear localization sequence-dependent nuclear protein import.
Yu
Lu-Gang
LG
Department of Medicine, The Henry Wellcome Laboratory of Molecular and Cellular Gastroenterology, University of Liverpool, Liverpool L69, United Kingdom.
Andrews
Nigel
N
Weldon
Mike
M
Gerasimenko
Oleg V
OV
Campbell
Barry J
BJ
Singh
Ravinder
R
Grierson
Ian
I
Petersen
Ole H
OH
Rhodes
Jonathan M
JM
D021581:Active Transport, Cell Nucleus; D000364:Agaricus; D001665:Binding Sites; D002463:Cell Membrane Permeability; D002846:Chromatography, Affinity; D003110:Colonic Neoplasms; D003600:Cytosol / Q000378:metabolism; D004072:Digitonin; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008024:Ligands; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
C041433:Agaricus lectins; D018840:HSP70 Heat-Shock Proteins; D037102:Lectins; D008024:Ligands; D011506:Proteins; C050087:oxygen-regulated proteins; D004072:Digitonin