Repetitive DNas of wild emmer wheat (Triticum dicoccoides) and their relation to S-genome species: molecular cytogenetic analysis.
We have analyzed the chromosomal GISH molecular banding patterns of three populations of the wild allopolyploid wheat Triticum dicoccoides in an attempt to unravel the evolutionary relationships between highly repetitive DNA fractions of T. dicoccoides and proposed diploid progenitors of the B genome. Aegilops speltoides showed almost complete affinity of its repetitive DNA to C-heterochromatin of T. dicoccoides, whereas other S-genome species demonstrated relatedness only to distal heterochromatin. This substantiates the priority of Ae. speltoides as the most similar to the wheat B-genome donor in comparison with other Sitopsis species. Using molecular banding technique with DNA of different Aegilops species as a probe permits tracing of the origin of each heterochromatin cluster. Molecular banding analysis reveals polymorphism between three wild emmer wheat populations. Comparison of molecular banding patterns with chromosomal distribution of the Ty1-copia retrotransposons, which constitute a large share of T. dicoccoides genome, makes it possible to propose that the activity of transposable elements may lie in the background of observed intraspecific polymorphism.
Raskina
Olga
O
Institute of Evolution, Haifa University, Mt. Carmel, Israel.
Belyayev
Alexander
A
Nevo
Eviatar
E
D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D020732:Cytogenetic Analysis; D015342:DNA Probes; D018744:DNA, Plant / Q000235:genetics*; D019143:Evolution, Molecular; D018745:Genome, Plant; D006570:Heterochromatin; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D011123:Polyploidy; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
D015342:DNA Probes; D018744:DNA, Plant; D006570:Heterochromatin; D018626:Retroelements