Expression of functional chemokine receptors by rat cerebellar neurons.
In this study, we examined chemokine receptor expression and function in rat cerebellar neurons. Calcium imaging experiments demonstrated that a wide variety of chemokines elicited [Ca(2+)](i) transients in acutely isolated and cultured cerebellar Purkinje and granule neurons. In many cases, these chemokine responses were pertussis toxin (PTX) insensitive. In addition, chemokines activated the Ca(2+) and cAMP-dependent transcription factor CREB and the extracellular response kinases ERK1/ERK2. Chemokines increased the survival of Purkinje neurons deprived of their trophic support. Thus, the presence of chemokine receptors and the signaling pathways they activate suggest that chemokines play a role in the control of cerebellar neuron survival and development and may mediate communication between the CNS and the immune system.
Gillard
Samantha E
SE
Department of Neuroscience, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 76285, USA.
Lu
Meiling
M
Mastracci
Rose M
RM
Miller
Richard J
RJ
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D018925:Chemokines / Q000494:pharmacology / Q000502:physiology; D019742:Chemokines, CC / Q000494:pharmacology; D004594:Electrophysiology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism*; D037342:Pertussis Toxin; D011689:Purkinje Cells / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D019707:Receptors, Chemokine / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014018:Tissue Distribution; D014764:Viral Proteins; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D018925:Chemokines; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D019707:Receptors, Chemokine; D014764:Viral Proteins; D010566:Virulence Factors, Bordetella; C108459:vMIP-1 protein, Human herpesvirus 8; C108059:vMIP-II; D037342:Pertussis Toxin; D002118:Calcium