Separation and identification of the phthalic anhydride derivatives of Liqusticum Chuanxiong Hort by GC-MS, TLC, HPLC-DAD, and HPLC-MS.
A simple, sensitive, and rapid method using gas chromatography (GC)-mass spectrometry (MS) is developed for the simultaneous separation and identification of the active ingredients of Liqusticum Chuanxiong Hort (Chuanxiong). Ten phthalic anhydride derivatives (PADs) are identified in Chuanxiong as 3-butylphthalide, 3-butylidenephthalide, 3-butylidene-4-hydroxyphthalide, senkyunolide A, neocnidilide, Z-ligustilide, E-ligustilide, senkyunolide F, senkyunolide-H, and senkyunolide-I. The existence of ferulic acid and vanillin in Chuanxiong extract is also demonstrated. Further identification of these compounds is performed by thin-layer chromatography, high-performance liquid chromatography (HPLC), and HPLC-MS analysis. This is the first report of the separation and determination of the PADs in Chuanxiong by GC-MS.
Li
Hong-Xia
HX
Department of Chemistry, Tsinghua University, Beijing, China.
Ding
Ming-Yu
MY
Yu
Jian-Yuan
JY
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010796:Phthalic Anhydrides / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D010796:Phthalic Anhydrides; D010936:Plant Extracts