title: Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1.
abstract: Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yielding 4-chlorocatechol, and 1,2,3,4-tetrachlorodibenzo-p-dioxin, producing 3,4,5,6-tetrachlorocatechol and 2-methoxy-3,4,5,6-tetrachlorophenol; all of these compounds were unequivocally identified by mass spectrometry both before and after N,O-bis(trimethylsilyl)-trifluoroacetamide derivatization by comparison with authentic standards. Additional experiments showed that strain RW1 formed a second metabolite, 2-methoxy-3,4,5,6-tetrachlorophenol, from the original degradation product, 3,4,5,6-tetrachlorocatechol, by methylation of one of the two hydroxy substituents.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Hyo-Bong
authors.initials: HB
authors.lastname: Hong
authors.forename: Yoon-Seok
authors.initials: YS
authors.lastname: Chang
authors.forename: In-Hyun
authors.initials: IH
authors.lastname: Nam
authors.forename: Peter
authors.initials: P
authors.lastname: Fortnagel
authors.forename: Stefan
authors.initials: S
authors.lastname: Schmidt
mesh_terms: D001711:Biotransformation; D002396:Catechols / Q000032:analysis; D004147:Dioxins / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism*
chemical_list: D002396:Catechols; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C423629:3,4,5,6-tetrachlorocatechol; C000925:2,7-dichlorodibenzo-4-dioxin; C092155:1,2,3,4-tetrachlorodibenzodioxin; C038861:4-chlorocatechol