title: Thrombomodulin expression in endothelial cells after contact with bone cement.
abstract: The expression of thrombomodulin after contact with CMW 1 bone cement extracts was studied in human umbilical vein endothelial cells. Cement extracts after 1 h and 7-day curing induced no significant variations in thrombomodulin antigen levels and in mRNA expression. Significant increase of thrombomodulin was observed when endothelial cells were treated with all-trans retinoic acid (ATRA). ATRA induced the increase of thrombomodulin also in cells incubated with cement extracts. These results suggest that CMW 1 bone cement does not impair the expression of thrombomodulin in endothelial cells.
authors.affiliation: Dipartimento Putti, Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cenni
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ciapetti
authors.forename: Donatella
authors.initials: D
authors.lastname: Granchi
authors.forename: Lucia
authors.initials: L
authors.lastname: Savarino
authors.forename: Alessandra
authors.initials: A
authors.lastname: Corradini
authors.forename: Melania
authors.initials: M
authors.lastname: Vancini
authors.forename: Leo Alessandro
authors.initials: LA
authors.lastname: Di
mesh_terms: D001843:Bone Cements / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D009499:Neutral Red / Q000494:pharmacology; D011485:Protein Binding; D011486:Protein C / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018180:Thrombomodulin / Q000096:biosynthesis*; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D001843:Bone Cements; D004396:Coloring Agents; D011486:Protein C; D012333:RNA, Messenger; D018180:Thrombomodulin; D009499:Neutral Red