[Gene expression significance of beta-catenin, p53 and PCNA in PJS polyposis].
OBJECTIVE
To study the significance of beta -catenin, p53 and PCNA in PJS harmatoma.


METHODS
Paraffin sections of 29 hamartomas of 18 patient with Peutz-Jeghers syndrome, 19 patients with colorectal mucosa and 10 persons with normal mucosa were examined using LSAB. Positive cells were detected under light microscope.


RESULTS
All 10 persons with normal mucosa showed p53 negative and beta - catenin positive. The beta - catenin protein was predominantly localized to the plasma membrane. PCNA index (PI) in normal mucosa was 10.56 +/- 7.51. The PI of hamartoma and cancer was 44.57 +/- 21.15 and 32.96 +/- 18.88. The PI of the two groups was significantly higher than that of the normal group. In hamartoma, the positive cells were mainly located in the low 1/3 part of the mucous glands. The positive rate of p53 was 24.1% (7/29) in hamartomatouspolyps and 57.9% (11/19) in colorectal carcinoma (chi(2) = 5.581, P < 0.05). Abnormal expression rate of beta- catenin in colorectal carcinoma was 73.7% (14/19) and 41.3% (12/29) in hamartomatous polyps. Some epithelial cells showed nuclear localization of beta- catenin and concentration of cytoplasm. (chi(2) = 4.825, P < 0.05).


CONCLUSIONS
The proliferation activity of hamartomatous polyps increased significantly by multifactory interaction. p53 and beta- catenin play a role in the early stage of hamartoma- adenoma-carcinoma sequence but have a different mechanism in inducing colorectal cancer.
Zhang
Wei
W
Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Meng
Ronggui
R
Fu
Chuangang
C
Yu
Dehong
D
D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006222:Hamartoma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010580:Peutz-Jeghers Syndrome / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis* / Q000235:genetics; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D018809:Proliferating Cell Nuclear Antigen; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53; D051176:beta Catenin