title: The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation.
abstract: The NIMA kinases are one of several families of kinases that participate in driving the eukaryotic cell cycle. NIMA-related kinases have been implicated in G2/M progression, chromatin condensation and regulation of the centrosome cycle. Here we report the identification of a new member of this family, FA2, from Chlamydomonas reinhardtii. FA2 was originally discovered in a genetic screen for deflagellation-defective mutants. We have previously shown that FA2 is essential for basal-body/centriole-associated microtubule severing. We now report that the FA2 NIMA-related kinase also plays a role in cell cycle progression in Chlamydomonas. This is the first indication that members of the NIMA family might exert their effects through the regulation of microtubule severing.
authors.affiliation: Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6.
authors.forename: Moe R
authors.initials: MR
authors.lastname: Mahjoub
authors.forename: Ben
authors.initials: B
authors.lastname: Montpetit
authors.forename: Lifan
authors.initials: L
authors.lastname: Zhao
authors.forename: Rip J
authors.initials: RJ
authors.lastname: Finst
authors.forename: Benjamin
authors.initials: B
authors.lastname: Goh
authors.forename: Apollos C
authors.initials: AC
authors.lastname: Kim
authors.forename: Lynne M
authors.initials: LM
authors.lastname: Quarmby
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins; D002469:Cell Separation; D048429:Cell Size; D002696:Chlamydomonas / Q000235:genetics* / Q000378:metabolism; D005407:Flagella / Q000378:metabolism*; D005434:Flow Cytometry; D017125:Genes, Protozoan; D006801:Humans; D008870:Microtubules / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D000072380:NIMA-Related Kinase 1; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D018797:Cell Cycle Proteins; D015800:Protozoan Proteins; C000606914:NEK1 protein, human; D000072380:NIMA-Related Kinase 1; D017346:Protein Serine-Threonine Kinases