[Effect of RU38486 on fibronectin expression in cultured human trabecular cells].
OBJECTIVE
To study the effect of RU38486 on fibronectin expression in human trabecular cells (HTCs) in vitro.


METHODS
The trabecular specimens from human donors were primarily cultured and subcultured. Cultured cells were observed by light and electron microscopes. Fibronectin (FN), laminin (LN) and neuron-specific enolase (NSE) in extracellular matrix (ECM) of the cells were immunohistochemically stained with LSAB method. The quantities of FN [optical density (A) means] affected by different concentrations of dexamethasone (Dex) and RU38486 were measured through indirect immunochemical method associated with computer image analysis.


RESULTS
According to the growing characteristics and morphological features, the cultured cells were identified as HTCs. Comparison of A means of FN induced by Dex and RU38486 was 10(-6) mol/L dex (12 days) > 10(-7) mol/L Dex (12 d) > 10(-7) mol/L Dex (5 d) > Control group = 10(-7) mol/L Dex (5 d) + 10(-8) mol/L RU38486 (7 d).


CONCLUSIONS
Dex can stimulate HTCs in vitro to secrete more FN. Bit RU38486 can reverse this high level of FN expression induced by Dex on receptor level. The efficacy of RU 38486 [glucocorticoid receptor (GR)-antagonist] in reducing intraocular pressure in vivo should be confirmed by further studies.
Li
Furong
F
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. champkee@163.net
Ye
Tiancai
T
Wang
Ningli
N
Zheng
Huling
H
D003907:Dexamethasone / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry; D005353:Fibronectins / Q000096:biosynthesis / Q000187:drug effects*; D005938:Glucocorticoids / Q000494:pharmacology; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D015735:Mifepristone / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D005353:Fibronectins; D005938:Glucocorticoids; D006727:Hormone Antagonists; D007797:Laminin; D015735:Mifepristone; D003907:Dexamethasone; D010751:Phosphopyruvate Hydratase