ZipA is required for recruitment of FtsK, FtsQ, FtsL, and FtsN to the septal ring in Escherichia coli.
The septal ring in Escherichia coli consists of at least nine essential gene products whose order of assembly resembles a mostly linear dependency pathway: FtsA and ZipA directly bind FtsZ polymers at the prospective division site, followed by the sequential addition of FtsK, FtsQ, FtsL, FtsW, FtsI, and FtsN. Recruitment of FtsK and all downstream components requires the prior localization of FtsA. Here we show that recruitment of FtsK, FtsQ, FtsL, and FtsN equally requires ZipA. The results imply that association of both FtsA and ZipA with FtsZ polymers is needed for further maturation of the nascent organelle.
Hale
Cynthia A
CA
Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4960, USA. cah19@po.cwru.edu
de Boer
Piet A J
PA
D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism / Q000502:physiology*; D002455:Cell Division; D004926:Escherichia coli / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015388:Organelles
D001426:Bacterial Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; C098711:FtsN protein, E coli; C068023:FtsQ protein, E coli; D008565:Membrane Proteins; C103795:ZipA protein, E coli; C078387:ftsL protein, E coli