ISMmy1, a novel insertion sequence of Mycoplasma mycoides subsp. mycoides small colony type.
A new insertion sequence, ISMmy1, has been identified in the bovine pathogen Mycoplasma mycoides subsp. mycoides biotype small colony (MmymySC). The occurrence of ISMmy1 in 15 MmymySC strains and 12 other mycoplasmas was examined by Southern blotting. All MmymySC strains showed identical hybridisation patterns except for the type strain PG1(T), the vaccine strain T1Sr49, and the strain Afadé, which all had unique patterns. ISMmy1-like sequences were also found in the bovine pathogen Mycoplasma bovis strain Donetta(T) while mycoplasmas that are phylogenetically closer to MmymySC lack ISMmy1. This observation suggests horizontal transfer between MmymySC and M. bovis.
Westberg
Joakim
J
Department of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
Persson
Anja
A
Pettersson
Bertil
B
Uhlén
Mathias
M
Johansson
Karl Erik
KE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D016415:Sequence Alignment
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009841:Oligonucleotides