title: [Effects of recombinant human leukemia inhibitor on the preimplantation mouse embryo development in vitro].
abstract: OBJECTIVE
  To assess the effect of recombinant human leukemia inhibitory factor (rhLIF) on mouse embryo development in vitro.
  
  
  METHODS
  Mice were randomly divided to three groups, one in vivo control (group I) and two in vitro (group II and III). Mice were sacrificed at 116 - 120 hours (group I) and 44 - 48 hours (group II and III) subsequent human chorionic gonadotropin (hCG) injection. Two-cell embryos (group II and III) and blastocysts (group I) were obtained. Embryos in group II were cocultured with human tubal fluid (HTF) + 10% human serum and in group III with HTF + 10% human serum + rhLIF (1 000 U/ml). The number of embryo in different stage was recorded and compared.
  
  
  RESULTS
  Embryo in four, eight-cell and morula was noted in group II and III, 87.7% versus 91.2% and 75.0% versus 85.4% respectively. There was no significant difference. However, further embryo development to the blastocyst, expanded blastocyst, and hatching blastocyst in group II (48.1%, 32.1% and 18.4%) was lower than that in group III (82.3%, 59.7% and 36.3%). There was no difference between blastocyst in group I and group III (86.0% vs 82.3%).
  
  
  CONCLUSIONS
  RhLIF does not provide obvious stimulation in early mouse embryo, however, rhLIF can promote the growth, differentiation, and hatching of preimplantion blastocyst.
authors.affiliation: Department of Obstetrics, Shandong Maternal and Child Health Hospital, Jinan 250014, China.
authors.forename: Guoyun
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Deng
authors.forename: Huiqin
authors.initials: H
authors.lastname: Zhang
authors.forename: Hongling
authors.initials: H
authors.lastname: Yu
authors.forename: Sen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000502:physiology; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011247:Pregnancy; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins