Synthesis of bacterial magnetic particles during cell cycle of Magnetospirillum magneticum AMB-1.
We investigated the relationship between the synthesis of bacterial magnetic particles (BMPs) and the transcription of magA gene-encoding iron transport protein using synchronous culture of Magnetospirillum magneticum AMB-1. Synchronously cultured cells were subjected to transmission electron microscopic observation and fluorescence in situ hybridization. The average number of BMPs slowly increased in the cell with increasing cell size. A sharp increase in BMPs occurred just before cell division and resulted in maximum BMP production of 30 particles/cell. The transcription of magA was regulated immediately before and after cell division.
Yang
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Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan.
Takeyama
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H
Tanaka
T
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Hasegawa
A
A
Matsunaga
T
T
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle; D052203:Ferrosoferric Oxide; D017404:In Situ Hybridization, Fluorescence; D007501:Iron / Q000378:metabolism; D008280:Magnetics; D008854:Microscopy, Electron; D010087:Oxides / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012244:Rhodospirillaceae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; C097085:MagA protein, Magnetospirillum; D010087:Oxides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D013440:Sulfides; C111959:greigite; D007501:Iron; D052203:Ferrosoferric Oxide