[Cloning of a novel gene, ANGPTL4 and the functional study in angiogenesis].
OBJECTIVE
To clone a novel HCC-related gene and study its functions.


METHODS
Using a combination of cell growth based functional screening of human placental cDNA library and RACE, a novel gene, angiopoietin-like 4 (ANGPTL4) was isolated. The gene was mapped by RH-PCR; also it was expressed with E. coli. Effects on angiogenesis was studied by MTT assay, in vitro wound migration assay, in vitro invasion assay, in vitro tube formation assay and in vivo mouse matrigel plug assay.


RESULTS
ANGPTL4 was cloned and mapped in 19p13.3. ANGPTL4 had no significant effect on proliferation of PAEC (P = 0.0504), while it can significantly stimulate migration and invasion of PAEC with the dose of 2 microgram/ml (P < 0.05) and promote in vitro tube formation of PAEC and angiogenesis of mouse in vivo.


CONCLUSIONS
ANGPTL4 can stimulate angiogenesis.
Zhu
Hongxin
H
National laboratory for oncogenes and related genes, Shanghai Cancer Institute, Shanghai 200032, China.
Li
Jinjun
J
Qin
Wenxin
W
Yang
Yanhua
Y
He
Xianghuo
X
Wan
Dafang
D
Gu
Jianren
J
D042682:Angiopoietins; D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000502:physiology*; D015723:Gene Library; D006133:Growth Substances / Q000737:chemistry; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D018919:Neovascularization, Physiologic / Q000502:physiology*
D042682:Angiopoietins; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; C118117:angiopoietin 4