[The effects of cytokines produced by bone marrow endothelial cells on the regulation of hematopoiesis].
In this study, the effects of bone marrow endothelial cell conditioned medium (BMEC-CM) on hematopoietic progenitors and the expression of cytokines mRNA of BMEC were investigated. BMEC-CM was ultrafiltrated in Centriprep-10, an ultrafiltering concentrator. The retentate (MW>10 kD) and filtrate (MW<10 kD) were obtained. The effects of different components of BMEC-CM on the murine hematopoietic progenitors were examined. The results showed that the retentate of BMEC-CM (MW>10 kD) significantly promoted the growth of CFU-GM, CFU-E, BFU-E, CFU-Meg and HPP-CFC, while the growth of CFU-GM, CFU-E, BFU-E, CFU-Meg and HPP-CFC were markedly inhibited by the filtrate of BMEC-CM (MW<10 kD). The effects of BMEC-CM were dose-dependent. The presence of mRNAs for GM-CSF, TGF-beta, bone morphogenetic protein 2A, basic fibroblast growth factor receptor, endothelin-2, macrophage-stimulating 1 (MSP-1), thymosin-beta10, connective tissue growth factor, macrophage inflammatory protein 2 alpha (MIP-2alpha), IL-6, IL-11, IL-13 and IFN-gamma mRNA were successfully detected by Atlas Arrays.
Li
W M
WM
Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078, China.
Huang
Y H
YH
Jiang
D Z
DZ
Wang
Q R
QR
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D054426:Chemokine CXCL2; D017077:Culture Media, Conditioned; D003583:Cytokinins / Q000096:biosynthesis*; D004727:Endothelium / Q000166:cytology / Q000378:metabolism*; D006410:Hematopoiesis / Q000502:physiology*; D017370:Interleukin-11 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D015846:Monokines / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013947:Thymosin / Q000096:biosynthesis* / Q000235:genetics; D000099201:Thymosin beta(4)
D054426:Chemokine CXCL2; D017077:Culture Media, Conditioned; D003583:Cytokinins; D017370:Interleukin-11; D015846:Monokines; D012333:RNA, Messenger; D013947:Thymosin; D000099201:Thymosin beta(4)