59Co solid-state NMR as a new probe for elucidating metal binding in polynucleotides.
Although magnesium fulfills several essential biochemical roles, direct studies on this ion are complicated by its unfavorable spectroscopic characteristics. This contribution explores the possibility of monitoring magnesium-nucleic acid binding via a combination of [Co(NH3)6]3+ as surrogate for [Mg(H2O)6]2+, and of high-resolution solid-state 59Co NMR as a spectroscopic probe. Such strategy quenches fast cationic exchanges between bound and free states, while exploiting the superior NMR properties of the 59Co spin. Experiments on relatively small amounts of tRNA can then discern resonances corresponding to different metal binding environments. These characterizations were assisted by studies on model compounds and by multinuclear 31P-59Co recoupling experiments.
Grant
Christopher V
CV
Department of Chemical Physics, Weizmann Institute of Sciences, 76100 Rehovot, Israel.
Frydman
Veronica
V
Harwood
John S
JS
Frydman
Lucio
L
D003036:Cobalt Isotopes; D008274:Magnesium / Q000737:chemistry* / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011119:Polynucleotides / Q000737:chemistry* / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry* / Q000378:metabolism
D003036:Cobalt Isotopes; D011119:Polynucleotides; D012343:RNA, Transfer; D008274:Magnesium