title: Kazal-type chymotrypsin inhibitor from duck pancreas.
abstract: A chymotrypsin inhibitor of the Kazal-type has been isolated from duck pancreas, by affinity chromatography on immobilized chymotrypsin, gel filtration on Bio-Gel P-10 and reverse phase (RP)-HPLC. It inhibits bovine chymotrypsin Aalpha with an association constant (K(a)) of 2.06x10(7) M(-1). The complete amino acid sequence was determined after digestion of pyridylethylated inhibitor with Staphylococcus aureus V8 protease and chemical cleavage with CNBr. Duck pancreatic chymotrypsin inhibitor (DPCI) was found to be a single polypeptide chain composed of 65 amino acid residues, corresponding to a molecular mass of 7191 Da.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50-137, Wroclaw, Poland. anna@bf.uni.wroc.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Wilimowska-Pelc
authors.forename: Zofia
authors.initials: Z
authors.lastname: Olichwier
authors.forename: Aleksander
authors.initials: A
authors.lastname: Mazurkiewicz
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kowalska
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Wilusz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D014359:Trypsin Inhibitor, Kazal Pancreatic; D002918:Chymotrypsin; C117608:alpha-chymotrypsin