In vivo characterization of the molecular-genetic changes in gastric mucosa during the development of acute gastritis and stress ulceration.
BACKGROUND
Trauma patients are at risk for the development of stress ulceration. Stress ulceration should be associated with increased heat-shock gene (iHSP70) and an inhibition of the trefoil peptide, spasmolytic polypeptide (SP), and mucin (MUC5AC) gene expressions.


METHODS
Male Sprague-Dawley rats (10 weeks old) were restrained for 0-, 4-, 8-, 12-, and 24-hour periods of time. Gastric ulcers were graded using a one- to three-point scoring system. The level of mucosal gene expression was determined at each time point for three genes: iHSP70, SP, and MUC5AC.


RESULTS
Gastric ulceration developed in direct proportion to the duration of restraint. Gastric ulceration was preceded by increased iHSP70 and decreased SP and MUC5AC gene expressions.


CONCLUSIONS
Restraint-induced gastric ulceration was preceded by an up-regulation of iHSP70 and a down-regulation of SP and MUC5AC gene expressions.
Choo
Darryl
D
Department of EMS/Trauma Surgery, Hartford Hospital, Hartford 06102-5037, USA.
Khwaja
Khalid
K
Nori
Kenneth
K
Rewinski
Michael
M
Zeff
Richard
R
Perdrizet
George
G
D000208:Acute Disease; D000818:Animals; D005753:Gastric Mucosa / Q000378:metabolism*; D005756:Gastritis / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006133:Growth Substances / Q000096:biosynthesis*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D055271:Mucin 5AC; D009077:Mucins / Q000096:biosynthesis*; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000235:genetics / Q000378:metabolism; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014456:Ulcer / Q000235:genetics* / Q000378:metabolism
D006133:Growth Substances; D018840:HSP70 Heat-Shock Proteins; C524680:Muc5ac protein, rat; D055271:Mucin 5AC; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; C099037:TFF3 protein, rat; C000604008:Tff2 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3