title: CD83 expression influences CD4+ T cell development in the thymus.
abstract: T lymphocyte selection and lineage commitment in the thymus requires multiple signals. Herein, CD4+ T cell generation required engagement of CD83, a surface molecule expressed by thymic epithelial and dendritic cells. CD83-deficient (CD83-/-) mice had a specific block in CD4+ single-positive thymocyte development without increased CD4+CD8+ double- or CD8+ single-positive thymocytes. This resulted in a selective 75%-90% reduction in peripheral CD4+ T cells, predominantly within the naive subset. Wild-type thymocytes and bone marrow stem cells failed to differentiate into mature CD4+ T cells when transferred into CD83-/- mice, while CD83-/- thymocytes and stem cells developed normally in wild-type mice. Thereby, CD83 expression represents an additional regulatory component for CD4+ T cell development in the thymus.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: LiLi
authors.initials: L
authors.lastname: Tu
authors.forename: Ann S
authors.initials: AS
authors.lastname: Miller
authors.forename: Cheryl
authors.initials: C
authors.lastname: Bock
authors.forename: Manabu
authors.initials: M
authors.lastname: Fujimoto
authors.forename: Carolyn
authors.initials: C
authors.lastname: Doyle
authors.forename: Douglas A
authors.initials: DA
authors.lastname: Steeber
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Tedder
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D015703:Antigens, CD; D016026:Bone Marrow Transplantation; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D019070:Cell Lineage / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics* / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D017154:Stromal Cells / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen