title: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of Delta(pH) and Delta(phi) in driving the ATP synthesis reaction.
abstract: The interface between the c-subunit oligomer and the a subunit in the F0 sector of the ATP synthase is believed to form the core of the rotating motor powered by the protonic flow. Besides the essential cAsp61 and aArg210 residues (Escherichia coli numbering), a few other residues at this interface, although nonessential, show a high degree of conservation, among these aGlu219. The homologous residue aGlu210 in the ATP synthase of the photosynthetic bacterium Rhodobacter capsulatus has been substituted by a lysine. Inner membranes prepared from the mutant strain showed approximately half of the ATP synthesis activity when driven both by light and by acid-base transitions. As estimated with the ACMA assay, proton pumping rates in the inner membranes were also reduced to a similar extent in the mutant. The most striking impairment of ATP synthesis in the mutant, a decrease as low as 12 times as compared to the wild-type, was observed in the absence of a transmembrane electrical membrane potential (Delta(phi)) at low transmembrane pH difference (Delta(pH)). Therefore, the mutation seems to affect both the mechanism responsible for coupling F1 with proton translocation by F0, and the mechanism determining the relative contribution of Delta(pH) and Delta(phi) in driving ATP synthesis.
authors.affiliation: Department of Biology, Laboratory of Biochemistry and Biophysics, University of Bologna, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Turina
authors.forename: B Andrea
authors.initials: BA
authors.lastname: Melandri
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D008027:Light; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases