Ca(v)2.3 (alpha1E) Ca2+ channel participates in the control of sperm function.
To know the function of the Ca2+ channel containing alpha(1)2.3 (alpha1E) subunit (Ca(v)2.3 channel) in spermatozoa, we analyzed Ca2+ transients and sperm motility using a mouse strain lacking Ca(v)2.3 channel. The averaged rising rates of Ca2+ transients induced by alpha-D-mannose-bovine serum albumin in the head region of Ca(v)2.3-/- sperm were significantly lower than those of Ca(v)2.3+/+ sperm. A computer-assisted sperm motility assay revealed that straight-line velocity and linearity were greater in Ca(v)2.3-/- sperm than those in Ca(v)2.3+/+ sperm. These results suggest that the Ca(v)2.3 channel plays some roles in Ca2+ transients and the control of flagellar movement.
Sakata
Yu
Y
Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, 113-8519, Tokyo, Japan.
Saegusa
Hironao
H
Zong
Shuqin
S
Osanai
Makoto
M
Murakoshi
Takayuki
T
Shimizu
Yasufumi
Y
Noda
Tetsuo
T
Aso
Takeshi
T
Tanabe
Tsutomu
T
D000818:Animals; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology*; D020908:Calcium Channels, R-Type; D020013:Calcium Signaling / Q000235:genetics / Q000502:physiology; D027682:Cation Transport Proteins; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009068:Movement / Q000502:physiology; D013081:Sperm Motility / Q000235:genetics / Q000502:physiology*; D013082:Sperm Tail / Q000502:physiology
C498916:Cacna1e protein, mouse; D015220:Calcium Channels; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins