BmTI antigens induce a bovine protective immune response against Boophilus microplus tick.
Boophilus microplus trypsin inhibitors (BmTIs) present in larvae were preliminarily characterized as active proteins, approximately 10-18 kDa, by SDS-PAGE. BmTIs showed trypsin inhibitory activity on reverse zymography containing gelatin (0.03%) and also inhibited others serine proteinases (human neutrophil elastase and human plasma kallikrein). Bos indicus, Nelore breed calves, previously sensitized with BmTIs and challenged with tick larvae (20,000 larvae/animal), showed 72.8% efficacy to interfere in tick development with 69.7% and 71.3% reduction of both tick number and egg weight, respectively. Cattle BmTls antiserum titer was approximately 1:8000. The maximum level of BmTls antibody production was detected 40 days after the first immunization by ELISA. Our preliminary results suggest that B. microplus serine proteinase inhibitors may play a role in the tick larvae fixation and feeding processes. Therefore, the development of antibodies against BmTIs might impair the normal parasitism.
Andreotti
Renato
R
Embrapa Gado de Corte, Campo Grande, MS, Brazil.
Gomes
Alberto
A
Malavazi-Piza
Kelly C
KC
Sasaki
Sergio D
SD
Sampaio
Claudio A M
CA
Tanaka
Aparecida S
AS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization / Q000379:methods / Q000662:veterinary; D007814:Larva / Q000276:immunology; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000662:veterinary*; D013987:Ticks / Q000254:growth & development / Q000276:immunology*; D013997:Time Factors; D014361:Trypsin Inhibitors / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D014361:Trypsin Inhibitors