title: Apoptosis, proliferation and p12(doc-1) profiles in normal, dysplastic and malignant squamous epithelium of the Syrian hamster cheek pouch model.
abstract: Disruption of the homeostatic balance between proliferation and apoptosis is widely believed to contribute to human oral carcinogenesis. Using the Syrian hamster oral cancer model, we examined normal, hyperplastic, dysplastic and malignant oral epithelium for the fraction of apoptotic, proliferating and p12(doc-1) expressing keratinocytes using the TUNEL assay, as well as PCNA and p12(doc-1) immunostaining, respectively. The percentage of TUNEL positive cells progressively increased from normal to dysplastic epithelium (P<0.0019), but returned to normal keratinocyte levels in the malignant epithelium (P<0.20). However, PCNA positive cells increased progressively through hamster oral malignant progression (P<0.0012). The overall ratio of apoptotic to proliferating keratinocytes remains similar until the transition between dysplastic and malignant epithelium, where the ratio is markedly reduced (P<0.05). p12(doc-1) labeling demonstrated a similar expression pattern (P<0.008). This study demonstrates that apoptosis, proliferation and the expression of p12(doc-1) reflects alterations reported during human oral carcinogenesis and supports the use of the Syrian hamster model for the further examination of these pathways.
authors.affiliation: Division of Oral Pathology, Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, Boston, MA 02115, USA.
authors.forename: Yohko
authors.initials: Y
authors.lastname: Kohno
authors.forename: Vipul
authors.initials: V
authors.lastname: Patel
authors.forename: Yong
authors.initials: Y
authors.lastname: Kim
authors.forename: Takanori
authors.initials: T
authors.lastname: Tsuji
authors.forename: Byung Rho
authors.initials: BR
authors.lastname: Chin
authors.forename: Moyi
authors.initials: M
authors.lastname: Sun
authors.forename: R
authors.initials: R
authors.lastname: Bruce Donoff
authors.forename: Ralph
authors.initials: R
authors.lastname: Kent
authors.forename: David
authors.initials: D
authors.lastname: Wong
authors.forename: Randy
authors.initials: R
authors.lastname: Todd
mesh_terms: D000818:Animals; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002610:Cheek; D006224:Cricetinae; D004848:Epithelium / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D020287:In Situ Nick-End Labeling; D008647:Mesocricetus; D008940:Mitotic Index; D023421:Models, Animal; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*; D025521:Tumor Suppressor Proteins / Q000032:analysis
chemical_list: C096151:CDK2AP1 protein, human; D025521:Tumor Suppressor Proteins