title: Proteolytic activity of YibP protein in Escherichia coli.
abstract: Escherichia coli YibP protein (47.4 kDa) has a membrane-spanning signal at the N-terminal region, two long coiled-coil regions in the middle part, and a C-terminal globular domain, which involves amino acid sequences homologous to the peptidase M23/M37 family. A yibP disrupted mutant grows in rich medium at 37 degrees C but not at 42 degrees C. In the yibP null mutant, cell division and FtsZ ring formation are inhibited at 42 degrees C without SOS induction, resulting in filamentous cells with multiple nucleoids and finally in cell lysis. Five percent betaine suppresses the temperature sensitivity of the yibP disrupted mutation. The mutant has the same sensitivity to drugs, such as nalidixic acid, ethidium bromide, ethylmethane sulfonate, and sodium dodecyl sulfate, as the parental strain. YibP protein is recovered in the inner membrane and cytoplasmic fractions, but not in the outer membrane fraction. Results suggest that the coiled-coil regions and the C-terminal globular domain of YibP are localized in the cytoplasmic space, not in the periplasmic space. Purified YibP has a protease activity that split the substrate beta-casein.
authors.affiliation: Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976, Japan.
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Ichimura
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Yamazoe
authors.forename: Maki
authors.initials: M
authors.lastname: Maeda
authors.forename: Chieko
authors.initials: C
authors.lastname: Wada
authors.forename: Sota
authors.initials: S
authors.lastname: Hiraga
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D010450:Endopeptidases / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000032:analysis
chemical_list: D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; C454699:EnvC protein, E coli