title: Immunohistochemical expression of SKALP/elafin in squamous cell carcinoma of human lung.
abstract: The immunohistochemical expression of skin-derived anti-leukoproteinase (SKALP)/elafin in lung squamous cell carcinoma (SqCC) and in uninvolved bronchial epithelium was studied. The results were compared with the immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and the TDT-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay. SKALP/elafin was expressed in SqCC with high incidence (82.6%). By contrast, the expression was not observed in uninvolved bronchial epithelium. SKALP/elafin expression was located in the cell layers just underneath the cornified envelope of each cell nest, and DNA fragmentation was observed in the same cell layers. PCNA was expressed in the basal layer of each cornified envelope, and both expressions were clearly separated. Immunoreactive score of SKALP/elafin expression was significantly correlated with the differentiation and it tended to increase in accordance with the degree of differentiation. These results suggested that SKALP/elafin was possibly involved in the cell differentiation program, finally leading to keratinization in SqCC of human lung. SKALP/elafin may be a beneficial molecular target for detection or even the development of a new therapeutic method against cancer.
authors.affiliation: Department of Surgery II, Kumamoto University Medical School, Kumamoto, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egami
authors.forename: Junichi
authors.initials: J
authors.lastname: Yamashita
authors.forename: Eiji
authors.initials: E
authors.lastname: Takai
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tamori
authors.forename: Noboru
authors.initials: N
authors.lastname: Fujino
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Kitaoka
authors.forename: Joost
authors.initials: J
authors.lastname: Schalkwijk
authors.forename: Michio
authors.initials: M
authors.lastname: Ogawa
mesh_terms: D000368:Aged; D001980:Bronchi / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002454:Cell Differentiation; D018572:Disease-Free Survival; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D016013:Likelihood Functions; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014176:Protein Biosynthesis; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D013997:Time Factors
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins