Expression of the adhesion molecule CEACAM1 (CD66a, BGP, C-CAM) in breast cancer is associated with the expression of the tumor-suppressor genes Rb, Rb2, and p27.
The adhesion molecule CEACAM1 (CD66a, BGP, C-CAM) is not only involved in maintaining normal tissue architecture, but also acts as a tumor suppressor in several experimental systems where loss of CEACAM1 expression results in enhanced tumor-cell growth and tumorigenicity. In order to further analyze the role of CEACAM1 in the development of breast cancer, we performed Western-blot analysis and immunohistochemistry with highly specific monoclonal antibodies in a cohort of 68 mammary carcinomas which had also been analyzed for expression of cell-cycle regulatory proteins cyclin D1, cyclin E, p16, p21, p27, Rb, and Rb2, as well as for steroid hormone receptor status, Ki67, and HER2/neu immunoreactivity. High CEACAM1 protein expression as found using both methods correlated significantly with expression of the retinoblastoma proteins Rb (P=0.004 and 0.013) and Rb2/p130 (P=0.003 and 0.007). In addition, we found a weak association of CEACAM1 expression with p27 protein levels (P=0.087 and 0.039), but with none of the other analyzed parameters. These results indicate the possibility of a functional link between cell-adhesion molecules and cell-cycle regulation that might play an important role in the development of mammary carcinomas.
Bamberger
Ana-Maria
AM
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
Kappes
Holger
H
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
Methner
Carola
C
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
Rieck
Gabriele
G
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
Brümmer
Jens
J
Department of Clinical Chemistry, University Hospital Eppendorf, Martinistr. 52, 20246 Hamburg, Germany, Germany.
Wagener
Christoph
C
Department of Clinical Chemistry, University Hospital Eppendorf, Martinistr. 52, 20246 Hamburg, Germany, Germany.
Löning
Thomas
T
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
Milde-Langosch
Karin
K
Department of Gynecopathology, Institute of Pathology, University Hospital Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany, Germany.
D015703:Antigens, CD / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002272:Carcinoembryonic Antigen; D002277:Carcinoma / Q000378:metabolism* / Q000556:secondary; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D015815:Cell Adhesion Molecules; D005260:Female; D016161:Genes, Retinoblastoma; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics; D008875:Middle Aged; D009124:Muscle Proteins; D016160:Retinoblastoma Protein / Q000096:biosynthesis* / Q000235:genetics; D056485:Retinoblastoma-Binding Protein 2; D025521:Tumor Suppressor Proteins
D015703:Antigens, CD; D000943:Antigens, Differentiation; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D002352:Carrier Proteins; C486183:Ceacam1 protein, mouse; D015815:Cell Adhesion Molecules; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D016160:Retinoblastoma Protein; C058992:Tagln protein, mouse; D025521:Tumor Suppressor Proteins; C070050:KDM5A protein, human; D056485:Retinoblastoma-Binding Protein 2