Artificial nucleases.
The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).
Chen
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CB
Molecular Biology Institute, University of California, Los Angeles Los Angeles, CA 90095-1570, USA.
Milne
L
L
Landgraf
R
R
Perrin
D M
DM
Sigman
D S
DS
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D003851:Deoxyribonucleases / Q000138:chemical synthesis* / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000187:drug effects
D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D046911:Macromolecular Substances; D010618:Phenanthrolines; D010710:Phosphates; C083120:phosphorodithioic acid; D004247:DNA; D006861:Hydrogen Peroxide; D003851:Deoxyribonucleases; C025205:1,10-phenanthroline