title: The EspB protein of enterohaemorrhagic Escherichia coli interacts directly with alpha-catenin.
abstract: Enterohaemorrhagic Escherichia coli (EHEC) belongs to a family of pathogens that cause attaching and effacing (A/E) lesion on target cells. The EspB protein of EHEC is translocated both to the host cell cytoplasm and to the membrane, and is essential for the signalling events leading to A/E lesion. To determine the actual role of EspB in this process, we tried to identify the EspB binding partner of the host cell protein, using a yeast two-hybrid assay, and obtained a cytoskeletal-associated protein, alpha-catenin. The alpha-catenin bound directly to the N-terminal region of EspB, both in solid (overlay assay) and solution (pull-down assay) phases, and it was recruited to the EHEC adherence site, dependent on EspB. Expression of the N-terminal region of EspB, as well as the whole EspB in host cells, inhibited F-actin accumulation on the adherence site. We conclude that EspB recruits alpha-catenin at the EHEC adherence site by direct interaction, and that the recruitment of alpha-catenin is essential for EHEC-induced A/E lesion formation.
authors.affiliation: Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kodama
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akeda
authors.forename: Gengo
authors.initials: G
authors.lastname: Kono
authors.forename: Akira
authors.initials: A
authors.lastname: Takahashi
authors.forename: Kinichi
authors.initials: K
authors.lastname: Imura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Iida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Honda
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D011994:Recombinant Proteins / Q000378:metabolism; D051178:alpha Catenin; D001616:beta-Galactosidase / Q000032:analysis
chemical_list: D001425:Bacterial Outer Membrane Proteins; C495273:CTNNA1 protein, human; D003598:Cytoskeletal Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D051178:alpha Catenin; D001616:beta-Galactosidase