[Changes in composition of cardiac myosin light chains in dilated cardiomyopathy: effect on functional properties].
A reduction (by 16-24%) in the amount of myosin regulatory light chains (LC2) in all heart sections of patients with dilated cardiomyopathy was found. The appearance of atrial essential light chains in ventricular myosin (up to 23%) not typical for this heart section in norm was also revealed. The decrease in LC2 content leads to a considerable inhibition of actin-activated ATPase activity and a loss of Ca2+ sensitivity of reconstructed filaments of myosin isolated from atria and ventricles of patients with dilated cardiomyopathy. The hybridization of myosin molecules from heavy chains of pathological human left ventricular myosin and light chains of pig left ventricular myosin leads to an increase in actin-activated ATPase activity of myosin and its Ca2+ sensitivity to the control level. The data suggest strongly the contribution of LC2-deficit to the distortion of functional properties of myosin in dilated cardiomyopathy. In contrast, the appearance of atrial LC1 in ventricle in dilated cardiomyopathy is a factor improving these properties.
Khalina
Ia N
IaN
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Udal'tsov
S N
SN
Podlubnaia
Z A
ZA
D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002311:Cardiomyopathy, Dilated / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D018994:Myosin Light Chains / Q000737:chemistry / Q000378:metabolism*; D013552:Swine
D000199:Actins; D018994:Myosin Light Chains; D000251:Adenosine Triphosphatases; D002118:Calcium