title: Probing degeneracy in antigen-antibody recognition at the immunodominant site of foot-and-mouth disease virus.
abstract: Antigen-antibody binding is regarded as one of the most representative examples of specific molecular recognition in nature. The simplistic view of antigenic recognition in terms of a lock-and-key mechanism is obsolete, as it is evident that both antigens and antibodies are flexible and can undergo substantial mutual adaptation. This flexibility is the source of complexities such as degeneracy and nonadditivity in antigenic recognition. We have used surface plasmon resonance to study the effects of combining multiple amino acid replacements within the sequence of the antigenic GH loop of foot-and-mouth disease virus. Our aim was 2-fold: to explore the extent to which antigenic degeneracy can be extended in this particular case, and to search for potential nonadditive effects in introducing multiple amino acid replacements. Combined analysis of one such multiply substituted peptide by SPR, solution NMR and X-ray diffraction shows that antigenic degeneracy can be expected as long as residues directly interacting with the paratope are conserved and the peptide bioactive folding is unaltered.
authors.affiliation: Department of Organic Chemistry, University of Barcelona, Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Gomes
authors.forename: E
authors.initials: E
authors.lastname: Giralt
authors.forename: W
authors.initials: W
authors.lastname: Ochoa
authors.forename: N
authors.initials: N
authors.lastname: Verdaguer
authors.forename: D
authors.initials: D
authors.lastname: Andreu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D000936:Antigen-Antibody Complex; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D010455:Peptides