Passive immunization with bovine milk containing antibodies to a cell surface protein antigen-glucosyltransferase fusion protein protects rats against dental caries.
Cell surface protein antigen (PAc) and glucosyltransferases (GTF) of Streptococcus mutans are major colonization factors of the organism. We prepared bovine milk containing antibodies against a fusion of the saliva-binding alanine-rich region of PAc with the glucan-binding domain of GTF-I. This study examined the effect of the immune milk on the cariogenicity of S. mutans in a rat model. Concentrated immune milk was fed to rats once a day for 55 days. The group that received immune milk had significantly less caries development than controls.
Mitoma
Morihide
M
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
Oho
Takahiko
T
Michibata
Naoko
N
Okano
Kaoru
K
Nakano
Yutaka
Y
Fukuyama
Masataka
M
Koga
Toshihiko
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D002417:Cattle; D003731:Dental Caries / Q000517:prevention & control*; D005964:Glucosyltransferases / Q000276:immunology*; D007116:Immunization, Passive; D008297:Male; D008562:Membrane Glycoproteins; D008892:Milk / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D013295:Streptococcus mutans / Q000201:enzymology / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; D005964:Glucosyltransferases