Sensitivity and specificity of ELISA and immunoblot for diagnosing neurocysticercosis.
In patients with neurocysticercosis (NCC), clinical manifestations and the results of neuroimaging procedures vary widely and often do not facilitate a definite diagnosis. In order to determine the value of immunodiagnosis for NCC, 222 serum and cerebrospinal fluid samples from patients with NCC and healthy subjects were examined. The samples represented patients from various endemic regions, those with other neurological disorders from an endemic area (Mexico), persons with various helminth infections other than NCC, and a group of healthy volunteers. All specimens were tested by enzyme-linked immunosorbent assay and immunoblot for the presence of Taenia solium-specific antibodies. The sensitivities of the enzyme-linked immunosorbent assay and the immunoblot test in NCC patients were almost identical (80% and 81.7%, respectively). For both tests, the sensitivity was higher when cerebrospinal fluid (86%) was tested compared with serum (75%). The overall specificity of enzyme-linked immunosorbent assay was only 75.3% because of frequent false-positive results in patients with other helminth infections, especially in those with echinococcosis. The specificity (99.4%) of the immunoblot test was clearly superior. It is concluded that enzyme-linked immunosorbent assay as a screening method and immunoblot as a confirmatory test contribute considerably to the diagnosis of NCC.
Gekeler
F
F
Neurologische Klinik Grosshadern, Klinikum der Ludwig-Maximilians-Universität München, Germany.
Eichenlaub
S
S
Mendoza
E G
EG
Sotelo
J
J
Hoelscher
M
M
Löscher
T
T
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000276:immunology; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology* / Q000469:parasitology; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology
D000909:Antibodies, Helminth; D007075:Immunoglobulin M