title: A lipoxygenase inhibitor from Aspergillus niger.
abstract: A lipoxygenase-1 (LOX-1) inhibitor was isolated from the fermented broth of Aspergillus niger CFTRI 1105. It was purified, using column and preparative thin layer chromatography. 1H NMR and GC-MS examination revealed the structure of the inhibitor to be 2-(2'-methyl, 4'-hydroxyphenyl), 2-(4"hydroxyphenyl)-propane with a molecular weight of 242 and the molecular formula C,6H18O2. This bisphenol-derivative inhibitor shows 50% inhibition of soybean LOX-I at 0.98 mM concentration. The activity of this inhibitor was compared with commercial bisphenol A and its structural analogues, butylhydroxyanisole and butylhydroxytoluene in an attempt to understand the role of functional groups affecting lipoxygenase activity.
authors.affiliation: Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, India.
authors.forename: Rao K C
authors.initials: RK
authors.lastname: Sekhar
authors.forename: Divakar K C
authors.initials: DK
authors.lastname: Sekhar
authors.forename: Rao A G
authors.initials: RA
authors.lastname: Appu
authors.forename: N G
authors.initials: NG
authors.lastname: Karanth
authors.forename: A P
authors.initials: AP
authors.lastname: Sattur
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight
chemical_list: D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase