title: Similar T helper Th2-like cytokine mRNA expression in vernal keratoconjunctivitis regardless of atopic constitution.
abstract: BACKGROUND
  Many patients with vernal keratoconjunctivitis (VKC), a severe chronic allergic eye disease in children, exhibit IgE-sensitization, but about 40% of cases lack this immunologic trait. As a disease factor in VKC, IgE is thus not fully understood. The aim of this study was to investigate whether there are any differences in the conjunctival cytokine messenger (m)RNA pattern related to IgE-sensitization in children suffering from VKC.
  
  
  METHODS
  Tissue samples were obtained from 16 symptomatic VKC subjects with sub-tarsal disease and six control subjects. Expression of mRNA for interleukin (IL)-4, IL-5, IL-13, and interferon (IFN)-gamma was investigated with a semiquantitative reverse-transcriptase polymerase chain reaction (RT-PCR) technique. The presence of T cells, IgE+ cells, mast cells, and eosinophils was analyzed with immunohistochemical methods. Allergen-specific IgE antibodies were assessed in serum and with skin prick testing.
  
  
  RESULTS
  Ten out of the 16 VKC subjects showed evidence of IgE-sensitization. No differences were detected for any tissue variable between VKC subjects with and without IgE-sensitization. Statistically significant increases over controls were found for both VKC groups with regard to all cell markers.
  
  
  CONCLUSIONS
  The amount of messenger RNA encoding cytokines and inflammatory cell markers in VKC did not correlate with IgE-sensitization. Tissue changes in all patient samples were characterized by a prevalence of T cells, eosinophils, mast cells and cell-bound IgE molecules. However, the role of cell-bound IgE molecules in VKC patients lacking IgE-sensitization remains to be determined.
authors.affiliation: Department of Clinical Sciences, Division of Ophthalmology, St. Erik's Hospital, Polhemsgatan 50, S-112 82 Stockholm, Sweden.
authors.forename: Per G
authors.initials: PG
authors.lastname: Montan
authors.forename: A
authors.initials: A
authors.lastname: Scheynius
authors.forename: I
authors.initials: I
authors.lastname: van der Ploeg
mesh_terms: D000293:Adolescent; D000485:Allergens / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D002648:Child; D002669:Child Welfare; D003233:Conjunctivitis, Allergic / Q000235:genetics* / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology*; D003888:Desensitization, Immunologic; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000378:metabolism*; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000276:immunology*; D012698:Serologic Tests; D013223:Statistics as Topic; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000485:Allergens; D016207:Cytokines; D012333:RNA, Messenger; D007073:Immunoglobulin E