title: Time-resolved X-ray diffraction reveals movement of F helix of D96N bacteriorhodopsin during M-MN transition at neutral pH.
abstract: D96N bacteriorhodopsin has two photointermediates with the deprotonated Schiff base: the M and MN intermediates. We measure the time-resolved x-ray diffraction of the D96N purple membrane after flash photoexcitation (pH 7.0, 25 degrees C). The data clearly show the M-MN transition during the D96N photocycle. Low-resolution projection maps of these states show that the F helix of the MN intermediate shifts from its original position and this shift is much larger than that of the M intermediate. This indicates that the F helix moves in the M-MN transition of the D96N bacteriorhodopsin photocycle. Moreover, the existence of the MN intermediate in the D96N photocycle under neutral pH indicates that the MN intermediate is not peculiar to the alkaline condition. It is notable that the structural transition of M-MN is independent of the protonation state of the Schiff base. Therefore, the F helix movement precedes reprotonation of the Schiff base in the bacteriorhodopsin photocycle. Our previous study showed that the M-MN transition is hydration-dependent and that the MN intermediate is more hydrated than the M intermediate. Considering this together with the present results, we conclude that the movement of the F helix causes hydration of the cytoplasmic side, which promotes the reprotonation of the Schiff base.
authors.affiliation: Department of Earth and Space Science, Faculty of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Oka
authors.forename: Naoto
authors.initials: N
authors.lastname: Yagi
authors.forename: Fumio
authors.initials: F
authors.lastname: Tokunaga
authors.forename: Mikio
authors.initials: M
authors.lastname: Kataoka
mesh_terms: D019943:Amino Acid Substitution; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000235:genetics*; D008968:Molecular Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D013997:Time Factors; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D011994:Recombinant Proteins; D001436:Bacteriorhodopsins