The novel exon, exon T, of the human progesterone receptor gene and the genomic organization of the gene.
Recently, we have cloned the novel isoform of the progesterone receptor (PR) cDNA (PR isoform S cDNA) from the human testicular cDNA library. The isoform S cDNA consists of the novel exon (termed the exon S of the PR gene) and the exons 4-8 of the PR gene. In order to investigate the existence of the other isoform of the human PR cDNA, the human testicular cDNA library was screened by the exons 4-8 corresponding sequence of the human PR cDNA in the present study. As a result, we have identified a novel isoform of the PR cDNA (termed the PR isoform T cDNA (PR-T cDNA)), which consisted of a previously unidentified 5'-sequence and the exons 4-8 of the PR gene. The structure of this isoform T cDNA is essentially similar to that of the isoform S cDNA. By the genomic cloning, the 5'-sequence of the PR isoform T mRNA was demonstrated to originate from a novel independent exon, exon T, which was located in the 5'-upstream region of the exon S.
Hirata
Shuji
S
Department of Obstetrics and Gynecology, Yamanashi Medical University, Shimokato 1110, Tamaho, Nakakoma 409-3898, Japan. shirata@swallow.res.yamanashi-med.ac.jp
Shoda
Tomoko
T
Kato
Junzo
J
Hoshi
Kazuhiko
K
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011980:Receptors, Progesterone / Q000235:genetics*; D013737:Testis / Q000378:metabolism
D018076:DNA, Complementary; D011980:Receptors, Progesterone