Occurrence of tumor antigen pRL1a specific CD8 T cells in spleen cells from syngeneic BALB/c, semiallogeneic (BALB/c x C57BL/6)F1 and allogeneic C57BL/6 mice.
We investigated the generation of CD8 cytotoxic T-lymphocytes (CTL) that recognized a dominant pRL1a peptide bound to H-2L(d) molecule on RL male 1 leukemia in spleen cells from RL male 1-bearing syngeneic BALB/c, semiallogeneic CB6F1 and allogeneic B6 mice by repetitive in vitro stimulation with RL male 1 tumor. CD8 T cells in cultures were also analyzed by H-2L(d)/pRL1a tetramer staining. We showed that pRL1a-specific CTL were more efficiently generated in spleen cells from RL male 1-bearing high responder CB6F1 mice than in low responder BALB/c mice, and this correlated well with the occurrence of H-2L(d)/pRL1a tetramer binding CD8 T cells. Furthermore, we showed that in spleen cells from RL male 1-bearing allogeneic B6 mice, H-2L(d)/pRL1a complex specific CD8 T cells were present at a significant frequency. H-2L(d)/pRL1a recognizing B6 CTL but not BALB/c or CB6F1 CTL gradually lost CD8 expression on their surface by multiplication of in vitro stimulation.
Hata
Hidenori
H
Department of Immunology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
Uenaka
Akiko
A
Takada
Itsuro
I
Kenjo
Akira
A
Takahashi
Minoru
M
Ono
Toshiro
T
Fujita
Teizo
T
Nakayama
Eiichi
E
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000737:chemistry*; D017252:CD3 Complex / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D015536:Down-Regulation; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009381:Neoplasms, Radiation-Induced / Q000276:immunology; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D017252:CD3 Complex; D016827:CD8 Antigens; D009842:Oligopeptides; D010455:Peptides; C465204:pRL1a protein, mouse