title: [Serial determination of crimidine by HPLC/SE/SM in a patient ingesting a "mouse trap"].
abstract: Crimidine (2 chloro, 4 methyl, 6 dimethyl amidopyrine) is a synthetic rodenticide which causes acute poisonings after oral ingestion in human. Major toxic effects are consciousness disorders, hypertonic coma and convulsions. Toxic level in human is about 5 mg/Kg. An intoxication case is reported. Five serums collected at different times were analyzed with HPLC/ES/MS. Crimidine was extracted with ethylacetate with recovery over 80%. Linearity was up to 800 micrograms/L. LOQ and LOD were 0.5 and 0.3 microgram/L respectively. The coefficients of variation were less than 10% for repeatability and reproductibility. Serum levels varied from 368 micrograms/L for H0 to 64 micrograms/L for H10 and elimination of crimidine was linear in time.
authors.affiliation: Unité Fonctionnelle de Toxicologie, Laboratoire de Biochimie et de Biologie Moléculaire, CHRU de Lille-59037 Lille.
authors.forename: T
authors.initials: T
authors.lastname: Besnard
authors.forename: N
authors.initials: N
authors.lastname: Sadeg
authors.forename: N
authors.initials: N
authors.lastname: Ricart
authors.forename: J
authors.initials: J
authors.lastname: Richecoeur
authors.forename: P
authors.initials: P
authors.lastname: Nisse
authors.forename: E
authors.initials: E
authors.lastname: Vinner
authors.forename: N
authors.initials: N
authors.lastname: Houdret
authors.forename: L
authors.initials: L
authors.lastname: Humbert
authors.forename: M
authors.initials: M
authors.lastname: Ihermitte
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D011743:Pyrimidines / Q000097:blood* / Q000493:pharmacokinetics / Q000506:poisoning*; D012378:Rodenticides / Q000097:blood* / Q000493:pharmacokinetics / Q000506:poisoning*; D013997:Time Factors
chemical_list: D011743:Pyrimidines; D012378:Rodenticides; C004579:crimidine