title: In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium.
abstract: OBJECTIVE
  Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium.
  
  
  METHODS
  Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection.
  
  
  RESULTS
  LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2. LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32.
  
  
  CONCLUSIONS
  Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.
authors.affiliation: Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
authors.forename: Yao-Yuan
authors.initials: YY
authors.lastname: Hsieh
authors.forename: Chich-Sheng
authors.initials: CS
authors.lastname: Lin
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Sun
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Horng-Der
authors.initials: HD
authors.lastname: Tsai
authors.forename: Jackson Chieh-Hsi
authors.initials: JC
authors.lastname: Wu
mesh_terms: D000818:Animals; D018076:DNA, Complementary; D004717:Endometrium / Q000502:physiology*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006131:Growth Inhibitors / Q000235:genetics*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D018076:DNA, Complementary; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008081:Liposomes; D008222:Lymphokines; D008156:Luciferases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases