Neurokinin NK1- and NK3-immunoreactive neurons in serotonergic cell groups in the rat brain.
Interactions are known to occur in the brain between serotonin (5-HT) and substance P (SP). To investigate whether SP can directly influence serotonergic neurons, double immunohistochemical labelings were performed on rat brain sections with NK1 or NK3 affinity-purified antibodies and a 5-HT monoclonal antibody. It was found that the vast majority of serotonergic cell bodies do not colocalize NK1 or NK3 labeling. Only in the central linear nucleus and ventral part of the dorsal raphe nucleus were a few serotonergic neurons double-labeled for NK1 receptors (15 and 0.8% of serotonergic neurons, respectively). It is suggested that serotonergic neurons are not major direct targets for SP in the rat brain.
Léger
Lucienne
L
INSERM U480, Institut Fédératif des Neurosciences de Lyon (IFNL 19), Faculté de Médecine, 8, Avenue Rockefeller, 69373 Lyon Cedex 08, France. leger@sommeil.univ-lyon1.fr
Gay
Nadine
N
Cespuglio
Raymond
R
D000818:Animals; D008297:Male; D009474:Neurons / Q000737:chemistry*; D011903:Raphe Nuclei / Q000737:chemistry*; D051381:Rats; D018040:Receptors, Neurokinin-1 / Q000032:analysis*; D018042:Receptors, Neurokinin-3 / Q000032:analysis*; D012701:Serotonin / Q000032:analysis / Q000502:physiology*; D013373:Substance P / Q000032:analysis
D018040:Receptors, Neurokinin-1; D018042:Receptors, Neurokinin-3; D012701:Serotonin; D013373:Substance P