title: A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus.
abstract: Tomato plants harboring the ripening-inhibitor (rin) mutation yield fruits that fail to ripen. Additionally, rin plants display enlarged sepals and loss of inflorescence determinacy. Positional cloning of the rin locus revealed two tandem MADS-box genes (LeMADS-RIN and LeMADS-MC), whose expression patterns suggested roles in fruit ripening and sepal development, respectively. The rin mutation alters expression of both genes. Gene repression and mutant complementation demonstrate that LeMADS-RIN regulates ripening, whereas LeMADS-MC affects sepal development and inflorescence determinacy. LeMADS-RIN demonstrates an agriculturally important function of plant MADS-box genes and provides molecular insight into nonhormonal (developmental) regulation of ripening.
authors.affiliation: U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) Plant, Soil and Nutrition Lab and Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Julia
authors.initials: J
authors.lastname: Vrebalov
authors.forename: Diane
authors.initials: D
authors.lastname: Ruezinsky
authors.forename: Veeraragavan
authors.initials: V
authors.lastname: Padmanabhan
authors.forename: Ruth
authors.initials: R
authors.lastname: White
authors.forename: Diana
authors.initials: D
authors.lastname: Medrano
authors.forename: Rachel
authors.initials: R
authors.lastname: Drake
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuch
authors.forename: Jim
authors.initials: J
authors.lastname: Giovannoni
mesh_terms: D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D016373:DNA, Antisense; D018076:DNA, Complementary; D005030:Ethylenes / Q000096:biosynthesis / Q000494:pharmacology; D005638:Fruit / Q000502:physiology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology*; D026161:MADS Domain Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D018514:Plant Structures / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; D005030:Ethylenes; D026161:MADS Domain Proteins; D010940:Plant Proteins; C036216:ethylene