title: [Synthesis and expression of 42 kD C-terminal region of the major merozoite surface protein (MSP1 - 42) of P. falciparum 3D7 strain in pichia pastoris].
abstract: OBJECTIVE
  Production of 3D7/MSP1 - 42 recombinant protein with correct conformation in Pichia pastoris for vaccine efficiency assay.
  
  
  METHODS
  Asymmetric PCR-based method was utilized to synthesize the 1 202 bp 3D7/msp1 - 42 gene. The expressing plasmid containing the synthetic gene was introduced into Pichia pastoris by electroporation. The secreted product was detected by Western Blot.
  
  
  RESULTS
  The redesigned entire 3D7/msp1 - 42 gene was generated with error-free, and expressed to produce 42 kD recombinant protein in secreted form. Conformational monoclonal antibody specific for MSP1 C-terminal can interact with the recombinant protein.
  
  
  CONCLUSIONS
  The redesigned 3D7/msp1 - 42 gene was expressed in P. pastoris with full length of recombinant protein which resembled most likely to the native protein.
authors.affiliation: Institute of Medical Biotechnology & Molecular Genetics of Second Military Medical University, Shanghai 200433 China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Weiqing
authors.initials: W
authors.lastname: Pan
authors.forename: Deru
authors.initials: D
authors.lastname: Lu
authors.forename: Liping
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000818:Animals; D018274:Electroporation / Q000379:methods; D020066:Merozoite Surface Protein 1 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D010843:Pichia / Q000235:genetics; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic
chemical_list: D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins