[Establishment and biological characteristics of nude mice xenograft tumor models of human endometrial carcinoma].
OBJECTIVE
To establish nude mice xenograft tumor models of human endometrial carcinoma for basic and clinical study.


METHODS
Fourteen samples of human endometrial carcinoma were subcutaneously heterotransplanted to nude mice (BALB/C, nu/nu), 3 approximately 4 mice were transplanted for every sample.


RESULTS
The initial take rate was 42.8%, then it increased to 100.0% after the 5th passage. Five of 14 samples were transplanted for 48 approximately 63 passages. The characteristics of histology, ultrastructure and chromosome were identical to those of human donor tumors. The DNA ploidy was consistent for SL-1, SL-2 and SL-3, but changed for SL-4 and SL-5 by cytometric analysis. The expression of estrogen receptor and progesterone receptor for 5 models was all negative. The expression of p53 was positive for SL-1, SL-2 and SL-4, but negative for SL-3 and SL-5. The expression of c-erbB-2 was positive for SL-1, SL-2 and SL-4, negative for SL-3 and SL-5. The expression of p16 was positive for SL-2 and SL-3, negative for SL-1, SL-4 and SL-5.


CONCLUSIONS
Five nude mice xenograft tumor models of human endometrial carcinoma were established successfully. It will be helpful for the basic and clinical study of human endometrial carcinoma.
Sheng
Xiugui
X
Department of Gynecological Oncology, Cancer Hospital, Institute of Shandong Province, Jinan 250117, China.
Sun
Jianheng
J
Zhou
Chunxiao
C
Wang
Shuzhen
S
D000818:Animals; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis; D004195:Disease Models, Animal; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D011003:Ploidies; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53