Comparative study of transfection efficiency of cationic cholesterols mediated by liposomes-based gene delivery.
To develop the efficient non-viral vector for gene delivery, we compared transfection activities of cationic cholesterol derivatives. We found that the stability of the liposome-DNA complex in the presence of endosome deeply related to the transfection efficiency. We also found that the introduction of a hydrophilic group to the amino terminal of the cholesterol derivative decreased stability and facilitated the release of DNA from the endosome, resulting in higher transfection efficiency.
Hasegawa
Seiji
S
Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, 467-8603, Nagoya, Japan.
Hirashima
Naohide
N
Nakanishi
Mamoru
M
D016475:3T3 Cells; D000818:Animals; D002412:Cations; D002784:Cholesterol / Q000008:administration & dosage* / Q000737:chemistry; D008081:Liposomes; D051379:Mice; D013050:Spectrometry, Fluorescence; D014162:Transfection
D002412:Cations; D008081:Liposomes; D002784:Cholesterol