title: Extraction by reversed micelles of the intracellular enzyme xylose reductase.
abstract: Xylose reductase enzyme (EC 1.1.1.21) produced by Candida guilliermondii in sugarcane bagasse was extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride cationic surfactant. An experimental design was employed to evaluate the influences of the following factors on the enzyme extraction: temperature, cosolvent, and surfactant concentration. A model was used to represent the enzyme recovery and fit of the experimental data. The extraction yielded a total recovery of 130%, and the purity increased 4.8-fold. This study demonstrates that liquid-liquid extraction by reversed micelles is a process able to recover and increase the enzymatic activity and purity of XR produced by C. guilliermondii.
authors.affiliation: Faculdade de Ciências Farmacêuticas/USP, São Paulo, SP, Brazil.
authors.forename: E V
authors.initials: EV
authors.lastname: Cortez
authors.forename: M C
authors.initials: MC
authors.lastname: Felipe
authors.forename: I C
authors.initials: IC
authors.lastname: Roberto
authors.forename: A
authors.initials: A
authors.lastname: Pessoa
authors.forename: M
authors.initials: M
authors.lastname: Vitolo
mesh_terms: D000449:Aldehyde Reductase / Q000096:biosynthesis / Q000302:isolation & purification*; D027461:Beta vulgaris; D018533:Biomass; D002175:Candida / Q000201:enzymology / Q000254:growth & development; D005285:Fermentation; D005511:Food Handling; D006868:Hydrolysis; D007220:Industrial Waste; D008823:Micelles; D008954:Models, Biological; D014993:Xylitol / Q000096:biosynthesis
chemical_list: D007220:Industrial Waste; D008823:Micelles; D000449:Aldehyde Reductase; D014993:Xylitol