title: Probing domain swapping for the neuronal SNARE complex with electron paramagnetic resonance.
abstract: Highly conserved soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins control membrane fusion at synapses. The target plasma membrane-associated SNARE proteins and the vesicle-associated SNARE protein assemble into a parallel four-helix bundle. Using a novel EPR approach, it is found that the SNARE four-helix bundles are interconnected via domain swapping that is achieved by substituting one of the two SNAP-25 helices with the identical helix from the second four-helical bundle. Domain swapping is likely to play a role in the multimerization of the SNARE complex that is required for successful membrane fusion. The new EPR application employed here should be useful to study other polymerizing proteins.
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: Dae-Hyuk
authors.initials: DH
authors.lastname: Kweon
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Michelle
authors.initials: M
authors.lastname: Poirier
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Kim
authors.forename: Yeon-Kyun
authors.initials: YK
authors.lastname: Shin
mesh_terms: D003497:Cyclic N-Oxides / Q000737:chemistry; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005583:Fourier Analysis; D008561:Membrane Fusion / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D050600:SNARE Proteins; D013113:Spin Labels; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins
chemical_list: D003497:Cyclic N-Oxides; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; D013113:Spin Labels; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins