Identification and functional analysis of an immunoreactive DsbA-like thio-disulfide oxidoreductase of Ehrlichia spp.
Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.
McBride
Jere W
JW
Department of Pathology and WHO Collaborating Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA. jemcbrid@utmb.edu
Ndip
Lucy M
LM
Popov
Vsevolod L
VL
Walker
David H
DH
D000818:Animals; D001483:Base Sequence; D004285:Dogs; D004536:Ehrlichia / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases / Q000032:analysis* / Q000235:genetics / Q000502:physiology
D019704:Protein Disulfide-Isomerases