Investigation of the role of ansocalcin in the biomineralization in goose eggshell matrix.
The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ovocleidin 17, and C-type lectins. Ansocalcin nucleates polycrystalline aggregates of calcite crystals in in vitro mineralization experiments. The polycrystalline aggregates obtained at higher concentration of ansocalcin appears to be similar to the crystals observed at the mamillary layer of the eggshell.
Lakshminarayanan
Rajamani
R
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117 543.
Kini
R Manjunatha
RM
Valiyaveettil
Suresh
S
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002119:Calcium Carbonate / Q000737:chemistry; D002845:Chromatography; D004527:Egg Proteins / Q000737:chemistry* / Q000378:metabolism; D004528:Egg Shell / Q000737:chemistry*; D005777:Geese; D037102:Lectins; D008855:Microscopy, Electron, Scanning; D008956:Models, Chemical; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D000596:Amino Acids; D004527:Egg Proteins; D037102:Lectins; D002119:Calcium Carbonate