[Expression of IL-6 and it's mRNA transcription in the process of rat periodontium remodeling influenced by various bite forces].
OBJECTIVE
To study the expression of IL-6 in periodontal ligament fibroblasts (PLF) and osteoblasts in order to explain the molecular mechanism of the bite forte on periodontium remodeling.


METHODS
A rat model of different bite forces was established to study the expression of IL-6 and IL-6 mRNA in the PLF and osteoblasts with immunohistochemical (IHC) and in situ hybridization (ISH) techniques.


RESULTS
In the group of disuse bite force, the expression of IL-6 and IL-6 mRNA in the PLF and osteoblasts enhanced apparently compared with that in the group of normal bite force. The gray level values enhanced respectively from 6.5886 +/- 1.6 to 38.8381 +/- 9.5 and from 11.8337 +/- 2.8 to 57.2623 +/- 13.6. The loosely arrangement of periodontal fibers and the resorption of alveolar bone were observed in histological study. In the group of compensation bite force, the same result was observed with IHC and ISH, the gray level values enhanced respectively from 6.3119 +/- 1.5 to 45.5456 +/- 11.1 and from 11.7714 +/- 2.8 to 81.9391 +/- 19.5, but the increase of periodontal fibers, the resorption and formation of alveolar bone were observed simultaneity in histological study.


CONCLUSIONS
With the change of bite force, the expression of IL-6 and IL-6 mRNA in PLF and osteoblasts presents a certain regulation. IL-6 may play important roles in the effect of bite force on periodontium remodeling.
Yuan
Lin
L
Department of Prosthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Zhao
Yunfeng
Y
Zhou
Weidong
W
D000818:Animals; D001732:Bite Force; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000032:analysis* / Q000235:genetics; D008297:Male; D010519:Periodontium / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D015850:Interleukin-6; D012333:RNA, Messenger