Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress.
By a transposon-tagging method, cadBA genes encoding a lysine/cadaverin antiporter and a lysine decarboxylase were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of cadBA were 64-97% similar to those reported from other Enterobacteriaceae. Functions of cadBA genes on acid tolerance were assessed by comparing acid tolerances of V. vulnificus and its isogenic mutants, whose cadBA genes were separately inactivated by allelic exchanges. The results demonstrated that gene products of cadBA contribute to acid tolerance of V. vulnificus, and that their contribution is dependent on prior exposure of cells to moderately acidic pH.
Rhee
Jee Eun
JE
Department of Food Science and Technology, Department of Molecular Biotechnology, Institute of Biotechnology, Chonnam National University, Kwang-Ju 500-757, South Korea.
Rhee
Joon Haeng
JH
Ryu
Phil Youl
PY
Choi
Sang Ho
SH
D026905:Amino Acid Transport Systems; D017920:Antiporters / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000235:genetics* / Q000254:growth & development*
D026905:Amino Acid Transport Systems; D017920:Antiporters; D001426:Bacterial Proteins; C463484:CadA protein, Vibrio vulnificus; C088101:CadB protein, Bacteria; D004269:DNA, Bacterial; D002262:Carboxy-Lyases; C021520:lysine decarboxylase