Progesterone receptor, bc1-2 and bax expression in meningiomas.
Meningiomas are generally benign central nervous system neoplasms, which frequently express progesterone receptor (PR) and only rarely express the estrogen receptor (ER). For breast cancer, a relation between steroid hormone receptors and proteins involved in the apoptotic process has been described. For meningiomas, the exact relation between PR and these proteins is not known. In this study, ER, PR, bcl-2 and bcl-2-associated x protein (Bax) expression levels were determined in meningioma cytosols. As a reference for our experimental conditions, we also determined these proteins in breast cancer cytosols. PR and ER were determined with a ligand-binding assay and scatchard-plot analysis. The expression levels of the anti- and pro-apoptotic proteins, bcl-2 and Bax, respectively, were determined by immunoblotting. In 65% of the meningioma, bcl-2 expression was found in variable amounts. In contrast to breast cancer, a significant negative association between PR and bcl-2 was found (P < 0.01). Bax expression appeared constitutive, not related to PR, and 2.6 times higher than breast cancer. As both PR and bcl-2 appear positively associated with prognosis, the negative relationship between bcl-2 and PR found in this study might have some biological and clinical significance.
Verheijen
F M
FM
Department of Endocrinology, University Medical Center Utrecht, The Netherlands. f.verheijen@twenteborg.nl
Donker
G H
GH
Viera
C Sales
CS
Sprong
M
M
Jacobs
H M
HM
Blaauw
G
G
Thijssen
J H H
JH
Blankenstein
M A
MA
D017209:Apoptosis; D001943:Breast Neoplasms; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008577:Meningeal Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D011960:Receptors, Estrogen / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D011980:Receptors, Progesterone / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D051028:bcl-2-Associated X Protein