Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1.
We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.
Ward
Donald E
DE
Laboratory for Microbiology, Wageningen University, 6703CT Wageningen, The Netherlands. deward@unity.ncsu.edu
Revet
Ingrid M
IM
Nandakumar
Renu
R
Tuttle
Jon H
JH
de Vos
Willem M
WM
van der Oost
John
J
DiRuggiero
Jocelyne
J
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D019711:Pyrococcus / Q000235:genetics*; D016415:Sequence Alignment
D004277:DNA, Single-Stranded