Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures.
The introduction of the epothilone polyketide synthase (PKS) into Myxococcus xanthus has enabled the heterologous production of epothilone D (1) on a large scale. To isolate this valuable product from the fermentation medium, an economical, scalable, and high-yielding purification process was developed. With the crystallization of 1 from a binary solvent system that consisted of ethanol and water, the product was recovered as white crystals with a final purity of > or =97% (w/w). This is the first reported crystallization of 1.
Arslanian
Robert L
RL
Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA. arslanian@kosan.com
Parker
Charles D
CD
Wang
Peter K
PK
McIntire
James R
JR
Lau
Janice
J
Starks
Courtney
C
Licari
Peter J
PJ
D002244:Carbon; D002625:Chemistry, Organic / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D034261:Epothilones; D004852:Epoxy Compounds / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009097:Multienzyme Complexes / Q000378:metabolism*; D016940:Myxococcus / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*
D034261:Epothilones; D004852:Epoxy Compounds; D009097:Multienzyme Complexes; D013844:Thiazoles; D002244:Carbon; C114026:desoxyepothilone B