title: The group B streptococcal C5a peptidase is both a specific protease and an invasin.
abstract: The group B streptococcus (GBS) is a major cause of pneumonia, sepsis, and meningitis in neonates and a serious cause of mortality or morbidity in immunocompromised adults. Although these streptococci adhere efficiently and invade a variety of tissue-specific epithelial and endothelial cells, adhesins and invasins are still unknown. All serotypes of GBS studied to date express C5a peptidase (SCPB) on their surface. This investigation addresses the possibility that this relatively large surface protein has additional activities. Rabbit anti-SCPB serum inhibited invasion of lung epithelial A549 cells by the serotype Ia strain O90R, suggesting that SCPB is an invasin. This was confirmed by inserting an in-frame 25-amino-acid deletion into the scpB gene. Invasion of HEp2 and A549 human cell lines was significantly reduced by the mutation. Enzyme-linked immunosorbent assays were used to demonstrate that purified SCPB protein binds directly to HEp2 and A549 cells and also binds the extracellular matrix protein fibronectin. Binding was dose dependent and saturable. These results suggested that SCPB is one of several potential invasins essential for GBS colonization of damaged epithelium.
authors.affiliation: Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Cheng
authors.forename: Deborah
authors.initials: D
authors.lastname: Stafslien
authors.forename: Sai Sudha
authors.initials: SS
authors.lastname: Purushothaman
authors.forename: Patrick
authors.initials: P
authors.lastname: Cleary
mesh_terms: D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology; D002478:Cells, Cultured; D010450:Endopeptidases / Q000502:physiology*; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D013292:Streptococcus agalactiae / Q000472:pathogenicity*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C078635:fibronectin-binding proteins, bacterial; C053476:invasin, Yersinia; D010450:Endopeptidases; C053088:C5a peptidase