title: Artificial nucleases.
abstract: The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).
authors.affiliation: Molecular Biology Institute, University of California, Los Angeles Los Angeles, CA 90095-1570, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Milne
authors.forename: R
authors.initials: R
authors.lastname: Landgraf
authors.forename: D M
authors.initials: DM
authors.lastname: Perrin
authors.forename: D S
authors.initials: DS
authors.lastname: Sigman
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D003851:Deoxyribonucleases / Q000138:chemical synthesis* / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D046911:Macromolecular Substances; D010618:Phenanthrolines; D010710:Phosphates; C083120:phosphorodithioic acid; D004247:DNA; D006861:Hydrogen Peroxide; D003851:Deoxyribonucleases; C025205:1,10-phenanthroline