title: Parallel synthesis of PNA-peptide conjugate libraries.
abstract: An optimized semi-automatic protocol for parallel synthesis of up to 96 peptide nucleic acids (PNA) or PNA-peptide conjugates using Boc-protection strategy has been developed using a robotic system. The approach is illustrated by synthesizing PNA and PNA-peptide libraries varying between 15 and 27  amino acid  units. The peptides (NLS (nuclear localization signal) or Tat-peptide) were attached to N-terminus of the PNA. The method was found to be far superior to that based on the SPOT/Fmoc protocol by which PNA oligomers are synthesized on a modified cellulose membrane. On a 0.5 micromole scale the method typically yielded 2 mg product of 90% purity by HPLC/MALDI-TOF analysis. This approach is suitable for screening of a large number of PNA and/or peptide sequences for biochemical and biological studies.
authors.affiliation: Center for Biomolecular Recognition, IMBG, Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, Copenhagen N, DK 2200, Denmark.
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Awasthi
authors.forename: Peter E
authors.initials: PE
authors.lastname: Nielsen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D019151:Peptide Library; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D010880:Piperidines / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D007202:Indicators and Reagents; D008567:Membranes, Artificial; D019151:Peptide Library; D020135:Peptide Nucleic Acids; D010880:Piperidines; D001618:beta-Lactamases