A radiation hybrid mapping panel for the rhesus macaque.
The genomes of nonhuman primates have recently become highly visible candidates for full genome analysis, as they provide powerful models of human disease and a better understanding of the evolution of the human genome. We describe the creation of a 5000 rad radiation hybrid (RH) mapping panel for the rhesus macaque. Duplicate genotypes of 84 microsatellite and coding gene sequence tagged sites from six macaque chromosomes produced an estimated whole genome retention frequency of 0.33. To test the mapping ability of the panel, we constructed RH maps for macaque chromosomes 7 and 9 and compared them to orthologous locus orders in existing human and baboon maps derived from different methodologies. Concordant marker order between all three species maps suggests that the current panel represents a powerful mapping resource for generating high-density comparative maps of the rhesus macaque and other species genomes.
Murphy
W J
WJ
Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702, USA. murphywi@mail.ncifcrf.gov
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J E
JE
Smith
C
C
Desrosiers
R C
RC
O'Brien
S J
SJ
D000818:Animals; D019143:Evolution, Molecular; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D023421:Models, Animal; D021101:Radiation Hybrid Mapping; D026801:Synteny / Q000235:genetics