title: An intron in the YRA1 gene is required to control Yra1 protein expression and mRNA export in yeast.
abstract: Yra1p is an essential and conserved mRNA export factor in yeast. Strikingly, removal of the intron from YRA1 causes a dominant-negative growth phenotype and a concomitant inhibition of mRNA export. However, both defects are neutralized by replacement of the intron of YRA1 by a different intron. Significantly, Yra1p is overproduced in yeast when expressed from its intronless gene, but Yra1p levels are the same as the wild type when expressed from an intron-containing YRA1 gene. Thus, an intron in YRA1 controls Yra1p expression and mRNA export.
authors.affiliation: BZH, Biochemie-Zentrum Heidelberg, D-69120 Heidelberg, Germany.
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Navarro
authors.forename: Katja
authors.initials: K
authors.lastname: Strässer
authors.forename: Ed
authors.initials: E
authors.lastname: Hurt
mesh_terms: D015536:Down-Regulation; D015966:Gene Expression Regulation, Fungal; D007438:Introns; D009687:Nuclear Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae