[Effects of recombinant human bone morphogenic protein-2 and hyaluronic acid on invasion of brain glioma in vivo].
OBJECTIVE
To investigate the effects of recombinant human bone morphogenic protein-2 (rhBMP-2) and hyaluronic acid (HA) on the invasion and metastasis of rat brain glioma in vivo.


METHODS
C6 rat glioma cells were transfected with the plasmid vector named pEGFP-N3 which contained an enhanced green fluorescent protein (EGFP) gene. The stable cell clones which expressed EGFP protein were stereotactically injected into the brain parenchyma of SD rats in order to establish a xenotransplanted tumor model. After rhBMP-2 and HA were administered in the xenotransplanted tumor model at different dosages, their effects on glioma invasion and metastasis were observed by pathology, flow cytometry fluoresclnec, and electronmicvoscopy.


RESULTS
EGFP-transfected C6 glioma cells gave off green fluorescence in vivo and in vitro. Area with tumor and area without tumor could be easily distinguished by fluorescence microscopy. Even distant micrometastasis and invasion at single-cell level could be detected. 10 microliter rhBMP-2 of the concentration of 5 microgram/ml inhibited C6 tumor invasion, while 10 microliter hyaluronic acid of the concentration of 100 microgram/ml evidently promoted the tumor invasion.


CONCLUSIONS
EGFP-transfected C6 cells can be transplanted into rats' brain so as to establish an excellent experimental animal model in the research on invasion and metastasis of brain glioma in vivo. RhBMP-2 inhibits tumor invasion, while HA evidently promotes its invasion.
Zhang
Xiang
X
The Neurosurgical Institute of PLA, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
Wu
Jingwen
J
Li
Xia
X
Fu
Luoan
L
Gao
Dakuan
D
Bai
Hongmin
H
Liu
Xianzhen
X
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000517:prevention & control* / Q000556:secondary; D049452:Green Fluorescent Proteins; D006801:Humans; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000627:therapeutic use; D014162:Transfection; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D049452:Green Fluorescent Proteins; D006820:Hyaluronic Acid