FIP: a highly automated beamline for multiwavelength anomalous diffraction experiments.
FIP is a French Collaborating Research Group (CRG) beamline at the European Synchrotron Radiation Facility (ESRF) dedicated exclusively to crystallography of biological macromolecules, with a special emphasis on multiwavelength anomalous diffraction data collection in the 0.7-1.81 A wavelength range. The optics, consisting of long cylindrical grazing-angle mirrors associated with a cryocooled double-crystal monochromator, delivers an optimal beam in the corresponding energy range. The high level of automation, which includes automated crystal centring, automated data-collection management and data processing, makes the use of this beamline very easy. This is illustrated by the large number of challenging structures that have been solved since 1999.
Roth
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Laboratoire de Cristallographie et Cristallogenèse des Protèines (LCCP), Institut de Biologie Structurale J.-P. Ebel CEA-CNRS, 41 Rue Jules Horowitz, 38027 Grenoble CEDEX 1, France.
Carpentier
P
P
Kaïkati
O
O
Joly
J
J
Charrault
P
P
Pirocchi
M
M
Kahn
R
R
Fanchon
E
E
Jacquamet
L
L
Borel
F
F
Bertoni
A
A
Israel-Gouy
P
P
Ferrer
J L
JL
D001331:Automation / Q000295:instrumentation / Q000379:methods; D002138:Calibration; D003461:Crystallography; D004581:Electronics; D005602:France; D005615:Freezing; D006801:Humans; D009250:NADP Transhydrogenases / Q000737:chemistry; D039502:Nuclear Cap-Binding Protein Complex / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000737:chemistry; D018123:Receptors, Interleukin / Q000737:chemistry; D012984:Software; D013050:Spectrometry, Fluorescence; D017356:Synchrotrons / Q000295:instrumentation*; D013696:Temperature; D014961:X-Ray Diffraction / Q000295:instrumentation* / Q000379:methods*
D039502:Nuclear Cap-Binding Protein Complex; D011506:Proteins; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D018123:Receptors, Interleukin; D009250:NADP Transhydrogenases