Identification of potential physiological activators of protein phosphatase 5.
The protein serine/threonine phosphatase designated PP5 has little basal activity, and physiological activators of the enzyme have never been identified. Purified PP5 can, however, be activated by partial proteolysis or by the binding of supraphysiological concentrations of polyunsaturated long-chain fatty acids to its tetratricopeptide repeat (TPR) domain. To test whether activation of PP5 by polyunsaturated but not saturated fatty acids was an artifact of the lower solubility of saturated fatty acids, the effects of fatty acyl-CoA esters were examined. Saturated and unsaturated long-chain fatty acids are both freely water-soluble when esterified to CoA. Long-chain fatty acyl-CoA esters activated PP5 at physiological concentrations, with the saturated compounds being more effective. We investigated the effects of chain length and of the CoA moiety on PP5 activation. Chains of 16 carbons or more were required for optimal activation, with no activation observed below 10 carbons. On the basis of competition studies using acetyl-CoA, the function of the CoA moiety appeared to be to increase solubility of the fatty acyl moiety rather than to interact with a specific binding site. These data suggested that long-chain fatty acid-CoA esters might be physiological activators of PP5 and point to a potential link between fatty acid metabolism and signal transduction via this enzyme. Because heat shock protein 90 is also known to bind to the TPR domain of PP5 via its C-terminal domain (C90), we investigated its effect on PP5 activity. C90 activated the enzyme approximately 10-fold. Thus, we have identified two potential physiological activators of PP5.
Ramsey
Andrew J
AJ
Department of Pharmacology, University of South Alabama, Mobile, Alabama 36688, USA.
Chinkers
Michael
M
D000214:Acyl Coenzyme A / Q000502:physiology; D000818:Animals; D016718:Arachidonic Acid / Q000502:physiology; D002413:Cations, Divalent; D020536:Enzyme Activators / Q000737:chemistry* / Q000494:pharmacology; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry / Q000502:physiology*; D005231:Fatty Acids, Unsaturated / Q000502:physiology; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology; D007700:Kinetics; D008274:Magnesium / Q000502:physiology; D008345:Manganese / Q000502:physiology; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000502:physiology; D010446:Peptide Fragments / Q000378:metabolism / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid; D013379:Substrate Specificity; D013552:Swine
D000214:Acyl Coenzyme A; D002413:Cations, Divalent; D020536:Enzyme Activators; D004952:Esters; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D018841:HSP90 Heat-Shock Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; C026563:arachidonyl-coenzyme A; D010171:Palmitoyl Coenzyme A; D016718:Arachidonic Acid; D008345:Manganese; C050466:lauroyl-coenzyme A; D010749:Phosphoprotein Phosphatases; C089641:protein phosphatase 5; D008274:Magnesium