Testing mouse mammary tumor virus superantigen as adjuvant in cytotoxic T-lymphocyte responses against a melanoma tumor antigen.
Cytotoxic T cells represent a powerful strategy for antitumor treatment. Depending on the route of injection, an important role for CD4 T cell-mediated help was observed in the induction of this response. For this reason, we investigated whether induction of a CTL response to the HLA-A2-restricted immunodominant peptide melanoma antigen Melan-A was improved by using rVVs expressing the CTL-defined epitope alone or in combination with an SAg. In the latter case, the few infected dendritic cells simultaneously presented an SAg and an antigen, i.e., peptide. Here, we show that the anti-Melan-A response was efficiently induced but not significantly improved by coexpression of the SAg.
Wirth
Susanne
S
Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.
Bille
Frédéric
F
Koenig
Sylvain
S
Wehrli
Nathalie
N
Miconnet
Isabelle
I
Lévy
Frédéric
F
Diggelmann
Heidi
H
Romero
Pedro
P
Acha-Orbea
Hans
H
D000276:Adjuvants, Immunologic; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D015234:HLA-A Antigens / Q000235:genetics; D015789:HLA-A2 Antigen; D008164:Luminescent Proteins / Q000235:genetics; D058965:MART-1 Antigen; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D008545:Melanoma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins; D018089:Superantigens / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics
D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D008164:Luminescent Proteins; D058965:MART-1 Antigen; C548668:Mlana protein, mouse; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D018089:Superantigens; D049452:Green Fluorescent Proteins