In electrospray ionization source hydrogen/deuterium exchange LC-MS and LC-MS/MS for characterization of metabolites.
A new method is described for performing hydrogen/deuterium (H/D) exchange in an electrospray ionization (ESI) source. The use of liquid chromatography (LC)-mass spectrometer equipped with an ESI source and deuterium oxide (D2O) as the sheath liquid allows H/D exchange experiments to be performed on-line. This directly provides information for determining the number and position of exchangeable hydrogens, aiding in the elucidation of the structures of drug metabolites. To demonstrate the utility of this method, LC-mass spectrometry (MS) and LC-MS/MS experiments were performed using either H2O or D2O as sheath liquid on a matrix metalloprotease (MMP) inhibitor (PD 0200126) and its metabolites. Examination of the mass shift of the deuteriated molecule from that of the protonated molecule allowed the number of exchangeable protons to be determined. Interpretation of the production-spectra helped to determine the location of the exchanged protons and assisted in the assignment of the site(s) of modification for each metabolite.
Lam
Wing
W
Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer Global Research and Development, Ann Arbor, Michigan 48105, USA.
Ramanathan
Ragu
R
D000818:Animals; D001711:Biotransformation; D001713:Biphenyl Compounds / Q000097:blood / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D003903:Deuterium / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D007202:Indicators and Reagents; D061965:Matrix Metalloproteinase Inhibitors; D009862:Online Systems; D011480:Protease Inhibitors / Q000097:blood / Q000493:pharmacokinetics*; D011817:Rabbits; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013449:Sulfonamides / Q000097:blood / Q000493:pharmacokinetics*
D001713:Biphenyl Compounds; D007202:Indicators and Reagents; D061965:Matrix Metalloproteinase Inhibitors; C452779:PD 0200126; D011480:Protease Inhibitors; D013449:Sulfonamides; D006859:Hydrogen; D003903:Deuterium