Isolation of Magnetospirillum magneticum AMB-1 mutants defective in bacterial magnetic particle synthesis by transposon mutagenesis.
Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 x 10(-7) per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analysis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.
Wahyudi
A T
AT
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan.
Takeyama
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H
Matsunaga
T
T
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D052203:Ferrosoferric Oxide; D007501:Iron / Q000378:metabolism*; D008280:Magnetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D010087:Oxides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012244:Rhodospirillaceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D004269:DNA, Bacterial; C097085:MagA protein, Magnetospirillum; D010087:Oxides; D013440:Sulfides; C111959:greigite; D007501:Iron; D052203:Ferrosoferric Oxide