Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and far-eastern Russia.
We compared the biological properties of Oshima 5-10 (tick-borne encephalitis [TBE] virus isolated in Hokkaido, Japan) and Sofjin-HO (Far-Eastern subtype TBE virus) including plaque formation, virus replication and virus protein synthesis in BHK-21 cell cultures to reveal strain differences. We also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. Plaques of Oshima 5-10 were smaller than those of Sofjin-HO. Virus titers in culture fluid of Oshima 5-10 were 1/100 of those of Sofjin-HO at 9 and 12 hr after infection. Less viral protein and RNA syntheses of strain Oshima 5-10 was observed than with Sofjin-HO. Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No difference between the two strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1.
Goto
Akiko
A
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
Hayasaka
Daisuke
D
Yoshii
Kentarou
K
Mizutani
Tetsuya
T
Kariwa
Hiroaki
H
Takashima
Ikuo
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D006224:Cricetinae; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D007564:Japan; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins