Molten globule structures in milk proteins: implications for potential new structure-function relationships.
Recent advances in the field of protein chemistry have significantly enhanced our understanding of the possible intermediates that may occur during protein folding and unfolding. In particular, studies on alpha-lactalbumin have led to the theory that the molten globule state may be a possible intermediate in the folding of many proteins. The molten globule state is characterized by a somewhat compact structure, a higher degree of hydration and side chain flexibility, a significant amount of native secondary structure but little tertiary folds, and the ability to react with chaperones. Purified alpha(s1)- and kappa-caseins share many of these same properties; these caseins may thus occur naturally in a molten globule-like state with defined, persistent structures. The caseins appear to have defined secondary structures and to proceed to quaternary structures without tertiary folds. This process may be explained, in part, by comparison with the architectural concepts of tensegrity. By taking advantage of this "new view" of protein folding, and applying these concepts to dairy proteins, it may be possible to generate new and useful forms of proteins for the food ingredient market.
Farrell
H M
HM
U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. hfarrell@arserrc.gov
Qi
P X
PX
Brown
E M
EM
Cooke
P H
PH
Tunick
M H
MH
Wickham
E D
ED
Unruh
J J
JJ
D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002614:Chelating Agents / Q000737:chemistry*; D002942:Circular Dichroism; D019791:Guanidine / Q000494:pharmacology; D007768:Lactalbumin; D008854:Microscopy, Electron; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins; D018832:Molecular Chaperones; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D017550:Spectroscopy, Fourier Transform Infrared
D002364:Caseins; D002614:Chelating Agents; D008894:Milk Proteins; D018832:Molecular Chaperones; D007768:Lactalbumin; D019791:Guanidine