title: Physical and genetic map of the Bacteroides fragilis YCH46 chromosome.
abstract: The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.
authors.affiliation: Department of Bacteriology, School of Medicine, The University of Tokushima, Tokushima 770-8503, Kuramoto-cho, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Mahfuzur Rahman
authors.initials: MR
authors.lastname: Sarker
authors.forename: Hideyo
authors.initials: H
authors.lastname: Ugai
authors.forename: Shigeru
authors.initials: S
authors.lastname: Akimoto
authors.forename: Syed Mohammed
authors.initials: SM
authors.lastname: Shaheduzzaman
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miki
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D001441:Bacteroides fragilis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D020161:Physical Chromosome Mapping
chemical_list: C078382:endodeoxyribonuclease AscI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases