Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.
The proteins under the control of the two-component system VirR/VirS in Clostridium perfringens were analyzed by using two-dimensional gel electrophoresis of the culture supernatant from the wild type and the virR mutant. Based on matrix-assisted laser desorption ionization-time of flight/mass spectrometry, seven positively regulated proteins and eight negatively regulated proteins were identified. Transcriptome analysis confirmed that 7 of the 15 proteins were regulated by the VirR/VirS system at the transcriptional level, but the remaining proteins were modified with a VirR/VirS-directed protease at the posttranslation and secretion levels. We purified and characterized the VirR/VirS-directed protease from the culture supernatant and identified it as a kind of clostripain. Because this proteolytic activity was strongly inhibited by leupeptin and antipain, it was concluded that this protease was a member of the family of cysteine proteases of C. perfringens.
Shimizu
Takeshi
T
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Ten-nohdai, Tsukuba 305-8575, Japan.
Shima
Kensuke
K
Yoshino
Ken-ichi
K
Yonezawa
Kazuyoshi
K
Shimizu
Tohru
T
Hayashi
Hideo
H
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D003016:Clostridium perfringens / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D020543:Proteome; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D020543:Proteome; C068267:mry protein, Streptococcus pyogenes; D012697:Serine Endopeptidases