title: Substrate spectrum of tyrocidine thioesterase probed with randomized peptide N-acetylcysteamine thioesters.
abstract: Apparent kinetic constants k(cat) and K(m) were determined for tyrocidine thioesterase (TycC TE) using randomized peptide N-acetylcysteamine thioesters as substrate analogues. The enzyme has been found to be adequately active for the synthesis of positional-scanning libraries for novel antibiotic screening with reduced k(cat)/K(m) in the range of 2 to 82 folds lower than that of the wild-type sequence
authors.affiliation: Department of Chemistry and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Kowloon, SAR, Hong Kong, China.
authors.forename: Guiyang
authors.initials: G
authors.lastname: Xie
authors.forename: Mahesh
authors.initials: M
authors.lastname: Uttamchandani
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Xianzhang
authors.initials: X
authors.lastname: Bu
authors.forename: San San
authors.initials: SS
authors.lastname: Lin
authors.forename: Ka Man
authors.initials: KM
authors.lastname: Wong
authors.forename: Weili
authors.initials: W
authors.lastname: Yan
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000493:pharmacokinetics; D004950:Esterases / Q000378:metabolism*; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014440:Tyrocidine / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D009842:Oligopeptides; D013438:Sulfhydryl Compounds; D014440:Tyrocidine; D004950:Esterases; C475899:tyrocidine thioesterase; D000111:Acetylcysteine