[The phospholipase A2 inhibitor, aristolochic acid, inhibits chloride secretion without altering barrier function].
OBJECTIVE
To study the effect of aristolochic acid (AA) and the effect of phospholipase A2 (PLA2) on barrier function and electrogenic chloride secretion in intestinal epithelium.


METHODS
Electrophysiological studies were performed in the T84 cell line and rat distal colon. Ionic secretion and transepithelial resistance were determined.


RESULTS
Exogenous AA increased calcium-stimulated secretion in the T84 cell line. Incubation of rat distal colon in the presence of AA, a PLA2 inhibitor, reduced basal ionic secretion without affecting transepithelial resistance and inhibited ionic secretion stimulated by a cyclic adenosine monophosphate (AMPc) agonist, forskolin. In T84 cells, AA inhibited both carbachol- and forskolin-stimulated secretion.


CONCLUSIONS
PLA2 modulates electrogenic chloride secretion but has no effect on barrier function in the T84 cell line or in rat distal colon.
Adame
Y
Y
Servicio de Cirugía I, Hospital Clínico San Carlos, Madrid, Spain.
Encinas
M
M
Alarma
P
P
Fernández-Represa
J A
JA
Mayol
J A
JA
D000818:Animals; D034341:Aristolochic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D002712:Chlorides / Q000502:physiology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley
D034341:Aristolochic Acids; D002712:Chlorides; C000228:aristolochic acid I; D010741:Phospholipases A; D054467:Phospholipases A2