Preventive administration of Mycobacterium tuberculosis 10-kDa heat shock protein (hsp10) suppresses adjuvant arthritis in Lewis rats.
Adjuvant arthritis (AA) can be induced in Lewis rats by immunization with Mycobacterium tuberculosis (Mt) in oil. We have investigated the modulation of AA by mycobacterial 10-kDa heat shock protein (hsp10), administered according to several protocols known to induce immune tolerance and immune deviation. Subcutaneous immunization with hsp10 in aqueous solution did not induce a cellular immune response, evaluated as delayed-type hypersensitivity (DTH) reaction, although anti-hsp10 antibodies, mainly of the IgG2a isotype, were detected in serum of treated animals. When rats were pretreated with hsp10 in aqueous solution before AA induction, no effects were seen on arthritis-induced joint swelling, although osteolysis and lymphocyte infiltration were slightly decreased. When other routes of administration were attempted, the strongest suppression was seen in the group of animals which received four intranasal (i.n.) administrations of protein and a subsequent challenge of hsp10 in incomplete Freund's adjuvant (IFA). We also found that the extent of disease suppression among the different groups of animals correlated with serum anti-hsp10 antibody levels. These antibodies mostly belonged to the IgG2a subtype, suggesting that immune deviation may play a role in the mechanism of disease suppression by hsp10.
Agnello
Davide
D
Mario Negri Institute for Pharmacological Research, Milan, Italy.
Scanziani
Eugenio
E
Di
Giancamillo Mauro
GM
Leoni
Flavio
F
Modena
Daniela
D
Mascagni
Paolo
P
Introna
Martino
M
Ghezzi
Pietro
P
Villa
Pia
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging / Q000276:immunology* / Q000517:prevention & control*; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology; D015519:Bone Density; D018835:Chaperonin 10 / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology; D007116:Immunization, Passive; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011859:Radiography; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018835:Chaperonin 10