Characterization of two CTR-like protein kinases in Rosa hybrida and their expression during flower senescence and in response to ethylene.
The expression of two CTR-gene homologues was investigated during flower senescence in two Rosa hybrida cultivars. A fragment of a gene for a protein kinase, termed RhCTR1 (GenBank Acc. No. AF271206), was amplified by PCR and used to isolate the corresponding full-length cDNA (Acc. No. AY032953) from a rose petal cDNA library. The protein RhCTR1 has 66% amino acid identity to Arabidopsis CTR1. A fragment of a second CTR homologue, termed RhCTR2 (Acc. No. AY029067) is 69% identical to the corresponding region of RhCTR1. RhCTR1 expression increased during flower senescence, while RhCTR2 was constitutively expressed during flower development. The expression of both RhCTR1 and RhCTR2 was increased in response to exogenous ethylene.
Müller
Renate
R
Department of Agricultural Science, Horticulture, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. renate.muller@kvl.dk
Owen
Carolyn A
CA
Xue
Zhong-Tian
ZT
Welander
Margareta
M
Stummann
Bjarne M
BM
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics*; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011494:Protein Kinases / Q000235:genetics*; D027826:Rosa / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; C036216:ethylene; D011494:Protein Kinases