In vivo temporal EPR imaging for estimating the kinetics of a nitroxide radical in the renal parenchyma and pelvis in rats.
The kinetics of a nitroxide radical in the renal parenchyma and pelvis in rats were investigated by employing an in vivo EPR imaging system equipped with a surface-coil-type resonator (SCR). The exposed kidney of a living rat was inserted into the single-turn coil of the SCR, with the renal major axis aligned with the direction of alternative magnetic field (B(1)). After the injection of nitroxide radical via the tail vein, EPR measurements were repeated. From the temporal EPR images of the kidney on the 2-D projection to the plane which is perpendicular to the direction of B(1,) the decay rate of nitroxide radical in the renal parenchyma and pelvis was estimated. The parenchymal decay rate was found to be significantly shorter than that for the pelvis.
Ueda
Atsushi
A
Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry, Yamagata, Japan.
Yokoyama
Hidekatsu
H
Nagase
Sohji
S
Hirayama
Aki
A
Koyama
Akio
A
Ohya
Hiroaki
H
Kamada
Hitoshi
H
D000818:Animals; D003497:Cyclic N-Oxides / Q000493:pharmacokinetics; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D007668:Kidney / Q000378:metabolism*; D007682:Kidney Pelvis / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D009589:Nitrogen Oxides / Q000493:pharmacokinetics*; D019047:Phantoms, Imaging; D011759:Pyrrolidines / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D011759:Pyrrolidines; C077426:3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl