title: Possible automatic cell classification of bone marrow aspirate using the CELL-DYN 4000 automatic blood cell analyzer.
abstract: In clinical hematology, the demand for bone marrow aspiration testing is increasing. However, conventional automatic blood cell analyzers cannot completely analyze erythroblasts, and evaluation has mainly been performed by visual examination (the microscopic method). Using the CELL-DYN 4000 automatic blood cell analyzer (CD4000) (Abbott Laboratories, North Chicago, IL), specific recognition and classification of erythroblasts by DNA staining is possible. In the present study, using bone marrow blood collected from normal subjects and patients with hematological malignancy, we classified cells by the microscopic method and with the CD4000, and compared the results. Good correlations were found for total nucleated cell count (TNCC), neutrophils, lymphocytes, erythroblasts, and the myeloid series to erythroid series (M/E) ratio. It is possible to detect blasts that emerge in patients with hematological malignancy using the blast flag system installed on the CD4000. Since all of the items can be analyzed in about 80 sec with the CD4000, cells in bone marrow aspirates can be classified faster with this apparatus than by the microscopic method. Therefore, analysis of bone marrow aspirates with this apparatus appears to be very useful not only for laboratory testing but also for clinical screening.
authors.affiliation: Department of Clinical Hematology, Osaka City University Medical School, Japan.
authors.forename: Ryousuke
authors.initials: R
authors.lastname: Yamamura
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamane
authors.forename: Masayuki
authors.initials: M
authors.lastname: Hino
authors.forename: Kensuke
authors.initials: K
authors.lastname: Ohta
authors.forename: Hisako
authors.initials: H
authors.lastname: Shibata
authors.forename: Izumi
authors.initials: I
authors.lastname: Tsuda
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Tatsumi
mesh_terms: D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells / Q000166:cytology*; D001856:Bone Marrow Examination; D002452:Cell Count / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008853:Microscopy