A novel reporter strain to follow Cre-mediated recombination in T and NK cells.
The Cre-loxP system permits the generation of mouse models in which the fate of a cell can be followed through time. Such approach is of great value in immunology because it may allow lineage studies and the dissection of the contribution of specific effector T cells to long-term memory responses or autoimmune responses. An essential component of such a strategy is the development of appropriate reporter strains of mice in which the inducible reporter molecule is not immunogenic and is well expressed at the cell surface of T cells. We describe here a novel reporter strain of mice that is designed to fulfill these criteria and show that this strain permits the monitoring of Cre-mediated recombination in both T cells and NK cells.
Wurtz
Olivier
O
Centre d'Immunologie de Marseille-Luminy, INSERM/CNRS/Université de la Méditérranée, Parc Scientifique de Luminy, 13288 Marseille Cedex 09, France.
Pophillat
Matthieu
M
Schmitt-Verhulst
Anne-Marie
AM
Guerder
Sylvie
S
D000818:Animals; D018801:CD2 Antigens / Q000235:genetics; D017930:Genes, Reporter; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D007694:Killer Cells, Natural / Q000201:enzymology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017384:Sequence Deletion; D013601:T-Lymphocytes / Q000201:enzymology*; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D018801:CD2 Antigens; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases