[Study of the role of calcitonin during the embryo implantation].
OBJECTIVE
To observe the role of calcitonin (CT) during the implantation.


METHODS
Human endometrial epithelial cells were cultured. After stimulated with various concentrations of CT, intracellular calcium (Ca(2+)) in the epithelial and stroma cells and pre-embryos were measured by the laser scanning confocal microscope.


RESULTS
When stimulated with different concentrations of CT, mean fluorescence levels in the epithelial cells were similar to that of the control. However CT can improve intracellar Ca(2+) of preimplantation embryos and stroma cells in a dose-dependent manner and significantly higher than those of controls. When 10 nmol/L CT was added to the culture medium, the intracellular Ca(2+) concentration in 8-cell embryos rose immediately. Embryo exposure to CT was followed by a series of Ca(2+) bursts that persisted for at least 2 hours. No change in Ca(2+) was observed when culture medium alone was added to the embryos. Pre-loading embryos and stoma cells with the Ca(2+) chelator, prevented the increased fluorescence after CT addition.


CONCLUSIONS
CT play an important role during the procesess of implantation. It maybe improve intracellar of preembryos and accerate the development of preembryos.
Fu
Zhihong
Z
Department of Obstetrics and Gynecology, Nangfang Hospital, The First Military Medical University, Guangzhou 510515, China.
Chen
Shiling
S
Xing
Fuqi
F
D001757:Blastomeres / Q000187:drug effects / Q000502:physiology; D002116:Calcitonin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004717:Endometrium / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011247:Pregnancy; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology
D002116:Calcitonin; D002118:Calcium