Rapid detection of Shigella species in environmental sewage by an immunocapture PCR with universal primers.
In the present study, we developed a quick, highly specific method for detection of Shigella species by combining immunocapturing of the bacteria and a universal primer PCR. The method drastically enhances test sensitivity, and it can be used not only for identification of Shigella species in the environment but also for rapid detection of other pathogens.
Peng
Xuanxian
X
The Key Laboratory of Education Ministry for Cell Biology & Tumor Cell Engineering, Department of Biology, School of Life Sciences, Xiamen University, Xiamen 361005, People's Republic of China. wangpeng@xmu.edu.cn
Luo
Wen
W
Zhang
Jianying
J
Wang
Sanying
S
Lin
Shengcai
S
D017931:DNA Primers; D004777:Environment; D016133:Polymerase Chain Reaction / Q000379:methods*; D012722:Sewage / Q000382:microbiology*; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D012722:Sewage