title: Evidence for the nitration of gamma-tocopherol in vivo: 5-nitro-gamma-tocopherol is elevated in the plasma of subjects with coronary heart disease.
abstract: This study investigated the hypothesis that nitration of gamma-tocopherol may be an important mechanism for the detoxification of reactive nitrogen oxide species in vivo. Using liquid chromatography-tandem MS we have shown that gamma-tocopherol can be nitrated in vivo to form 5-nitro-gamma-tocopherol and that concentrations of this compound are elevated in the plasma of subjects with coronary heart disease. In addition, we demonstrate in carotid-artery atherosclerotic plaque that nitration of gamma-tocopherol is also evident at levels similar to that seen in the plasma of subjects with coronary heart disease.
authors.affiliation: Department of Medicine, University of Western Australia and Western Australian Institute for Medical Research, PO Box X2213, GPO Perth, Western Australia, 6001, Australia.
authors.forename: Lincoln W
authors.initials: LW
authors.lastname: Morton
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Ward
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Croft
authors.forename: Ian B
authors.initials: IB
authors.lastname: Puddey
mesh_terms: D015415:Biomarkers / Q000097:blood; D002340:Carotid Artery Diseases / Q000097:blood; D003327:Coronary Disease / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry; D012016:Reference Values; D024505:Tocopherols / Q000097:blood; D024504:gamma-Tocopherol / Q000031:analogs & derivatives* / Q000097:blood* / Q000138:chemical synthesis
chemical_list: C406664:5-nitro-gamma-tocopherol; D015415:Biomarkers; D024504:gamma-Tocopherol; D024505:Tocopherols