Effect of synthetic beta-endorphin-like peptide immunorphin on human T lymphocytes.
Beta-endorphin and the synthetic beta-endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met(5)]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of beta-endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of (125)I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors (K(d) = 7.0 +/- 0.3 nM). Unlabeled immunorphin completely inhibits (125)I-labeled beta-endorphin specific binding to naloxone-insensitive receptors on T lymphocytes (K(i) = 0.6 +/- 0.1 nM). Thus, beta-endorphin and immunorphin interact with common naloxone-insensitive receptors on T lymphocytes.
Navolotskaya
E V
EV
Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow Region, 142290 Russia. navolots@fibkh.serpukhov.su
Malkova
N V
NV
Zargarova
T A
TA
Lepikhova
T N
TN
Krasnova
S B
SB
Lipkin
V M
VM
D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D003208:Concanavalin A / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology; D006801:Humans; D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D007457:Iodine Radioisotopes / Q000737:chemistry; D008213:Lymphocyte Activation; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D011957:Receptors, Opioid / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D001615:beta-Endorphin / Q000494:pharmacology*
D007127:Immunoglobulin Constant Regions; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D007457:Iodine Radioisotopes; D009292:Narcotic Antagonists; D009842:Oligopeptides; D010446:Peptide Fragments; D011957:Receptors, Opioid; C445661:immunorphin; D003208:Concanavalin A; D009270:Naloxone; D004744:Enkephalin, Methionine; D001615:beta-Endorphin