hNT neurons express an immunosuppressive protein that blocks T-lymphocyte proliferation and interleukin-2 production.
Ntera2/D1 cells had an A1 B8 Bw6 Cw7 DR3 DR52 major histocompatibility complex (MHC) genotype. Its neuronal derivative, hNT neurons, expressed A1 B8 Bw6 MHC class I molecules, but did not activate, and its hNT supernatant suppressed allogeneic mixed lymphocyte cultures (MLC) >98% (p<0.01), phytohemagglutinin (PHA)-activated T-cell proliferation >87% (p<0.01), even 48 h after stimulation, suppressed phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced T-cell proliferation >99% (p<0.001), and reduced interleukin-2 (IL-2) production (p<0.01), while maintaining T cells in a quiescent G(0)/G(1) state without lowering their viability. This immunosuppressive activity was attributed to a 40-100-kDa anionic hNT protein with an isoelectric point of 4.8.
Gower
W R
WR
Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
Sanberg
P R
PR
Brown
P G
PG
Salyani
S
S
Pasqual
C J
CJ
McGrogan
M
M
Good
R A
RA
Engelman
R W
RW
D016380:Brain Tissue Transplantation / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D017077:Culture Media, Conditioned; D015870:Gene Expression / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000276:immunology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000276:immunology; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2