[Effect of trypsin on the rat keratinocyte separation and subculture].
The effect of trypsin on the separation an subculture of the keratinocytes was investigated in this work. It was found that when 0.25% trypsin was employed for 5 minutes to separate keratinocytes, the number of active keratinocytes and the cells capable of forming colony were higher than those of other experimental conditions. The maximum attached ratio of primary keratinocytes was obtained when skin tissues were treated at 0.05% concentration of trypsin. With the increase of the trypsin concentrations, the attached ratio, attachment rate constant, and colony forming efficiency were all increased. Thus, 0.25% concentration of trypsin was recommended for separating and subculturing the keratinocytes.
Ouyang
An-Li
AL
State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 200237, China.
Zhou
Yan
Y
Hua
Ping
P
Tan
Wen-Song
WS
D000818:Animals; D002452:Cell Count / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D013234:Stem Cells / Q000187:drug effects*; D014357:Trypsin / Q000494:pharmacology*
D014357:Trypsin