Purification of soluble murine transplantation antigens by isoelectric focusing.
Hydrosoluble transplantation antigens were prepared from membranes and microsomes of C(3)H mice (BP8 tumoral cells) and purified by isoelectric focusing. A biologically active fraction which seems homogenous by acrylamide gel electrophoresis was characterized: it specifically inhibits anti C(3)H hemagglutinin antibodies and provokes a highly significant prolongation of skin graft.
Jolles
P
P
Laboratory of Biochemistry, Faculty of Science, 96 Bd. Raspail, 6o, Paris, France
Schoentgen
F
F
Halle-Pannenko
O
O
Martyre
M -C.
M