[A study of expression of cell cycle genes in Drosophila using an enhancer trap and radioautography].
The phase of expression of genes CycB, CycE, and chb were determined in the cell cycle of neuroblasts of D. melanogaster 3rd instar larvae using the previously described radioautographic method and software. CycB was expressed at G2 phase and upon transition from G2 phase to M phase, while CycE was expressed at the end of G1 phase and upon transition from G1 phase to S phase. The phase of expression of the centrosome-associated protein was determined more precisely in G2 phase. The mean life span of reporter beta-galactosidase in neuroblasts was 4 h. The existence of more than one peak of expression of the gene in question in the cell cycle is discussed.
Trunova
S A
SA
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Prosp. akad. Lavrent' eva 10, Novosibirsk, 630090 Russia.
Dubatolova
T D
TD
Omel'ianchuk
L V
LV
D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004742:Enhancer Elements, Genetic; D005260:Female; D017952:Ganglia, Invertebrate / Q000166:cytology* / Q000502:physiology; D005821:Genetic Techniques; D007814:Larva; D008297:Male
D029721:Drosophila Proteins