[Effect of cyclosporin A on the growth of human first trimester cytotrophoblasts in vitro].
OBJECTIVE
To investigate effect of cyclosporin A (CsA) on the growth of first trimester human cytotrophoblast in vitro.


METHODS
To isolate and purificate first trimester human cytotrophoblasts by way of percoll density gradient centrifugation; to evaluate the effect of CsA on in vitro proliferation of cytotrophoblasts using (3)H-thymidine incorporation assay; to analyze of cell cycle of cytotrophoblasts with flow cytometric assay; and to survey morphology of the cells by scanning electro-microscope respectively.


RESULTS
CsA could increase in vitro proliferation of first trimester human cytotrophoblasts when concentration of CsA ranged from 1 x 10(-4) micromol/L to 1 micromol/L, but decrease in vitro proliferation of the cells if the concentration reached to 10 micromol/L. G(2)-M phase increased from (2.8 +/- 0.5)% to (6.7 +/- 1.3)% when the cytotrophoblasts were incubated with culture medium containing 1 micromol/L CsA for 24 hours, while S phase increased from (14 +/- 5)% to (28 +/- 8)% and apoptosis decreased from (8.7 +/- 2.2)% to (3.6 +/- 1.0)% when the culture in the same incubating condition lasted for 48 hours. Pseudopodia of the trophoblasts increased in number and length when they were incubated in culture medium containing 1 micromol/L of CsA for 48 hours, but decreased if the concentration reached to 10 micromol/L with the same incubating duration.


CONCLUSIONS
CsA is able to promote growth of first trimester human cytotrophoblasts and change their morphology, which appears to increase in invasive ability.
Yan
Fengting
F
Laboratory of Reproductive Jmmunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
Li
Dajin
D
Sun
Xiaoxi
X
Zhu
Ying
Y
Wang
Mingyan
M
Meng
Yi
Y
Yu
Jiang
J
D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D016196:S Phase / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects*
D007166:Immunosuppressive Agents; D016572:Cyclosporine