title: Expression of pituitary adenylate cyclase-activating polypeptide and PACAP type 1 receptor in the rat gastric and colonic myenteric neurons.
abstract: Pituitary adenylate cyclase-activating polypeptide (PACAP) is known to regulate gastric acid secretion and intestinal motility. In the present study, the pattern of distribution of PACAP and PACAP type 1 receptor (PAC1) immunoreactivities were examined in the rat stomach and distal colon using a specific polyclonal antibody raised against rat/human PAC1. Western blot of the membrane preparations of NIH/3T3 cells transfected with the human PAC1 obtained by using rabbit polyclonal anti-PAC1 antibody showed a protein band with a molecular mass of approximately 50 kDa. NIH/3T3 cells transfected with the human PAC1 and incubated with the anti-PAC1 antibody displayed surface cell-type immunoreactivity, which was internalized following ligand exposure. In gastric or colonic longitudinal muscle/myenteric plexus (LMMP) whole mount preparations as well as cryostat sections, PACAP immunoreactivity was observed in cell bodies within the myenteric ganglia and nerve fibers in the muscle layers and mucosa. PAC1 immunoreactivity was confined mainly on the surface of the nerve cells. PACAP and PAC1 immunoreactivities showed a similar pattern of distribution in gastric and colonic tissues. Adjacent sections or LMMP whole mount preparations labeled with protein gene product 9.5 (PGP 9.5) revealed the neuronal identity of myenteric cells bearing PAC1. The neuronal localization of PACAP and PAC1 receptors supports their role in the neural regulation of gastric acid secretion and gastrointestinal motor function.
authors.affiliation: CURE: Digestive Diseases Research Center, VA Greater Los Angeles Healthcare System and Department of Medicine, University of California, Los Angeles, 90073, USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Miampamba
authors.forename: Patrizia M
authors.initials: PM
authors.lastname: Germano
authors.forename: Selin
authors.initials: S
authors.lastname: Arli
authors.forename: Helen H
authors.initials: HH
authors.lastname: Wong
authors.forename: David
authors.initials: D
authors.lastname: Scott
authors.forename: Yvette
authors.initials: Y
authors.lastname: Taché
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Pisegna
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D003106:Colon / Q000737:chemistry* / Q000294:innervation*; D017615:Enteric Nervous System / Q000737:chemistry* / Q000166:cytology; D005753:Gastric Mucosa / Q000737:chemistry* / Q000294:innervation*; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009197:Myenteric Plexus / Q000737:chemistry / Q000166:cytology; D009474:Neurons / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017207:Rats, Sprague-Dawley; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D011979:Receptors, Pituitary Hormone / Q000032:analysis*; D013869:Thiolester Hydrolases / Q000032:analysis; D043222:Ubiquitin Thiolesterase
chemical_list: C495402:Adcyap1 protein, mouse; C495403:Adcyap1 protein, rat; C495504:Adcyap1r1 protein, mouse; C495505:Adcyap1r1 protein, rat; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D051237:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; D011979:Receptors, Pituitary Hormone; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase