title: Frequent up-regulation of WNT5A mRNA in primary gastric cancer.
abstract: WNT signal is transduced to the beta-catenin - TCF pathway, the JNK pathway, or the Ca2+-releasing pathway through seven-transmembrane-type WNT receptors encoded by Frizzled genes (FZD1-FZD10). We have previously cloned and characterized human WNT2B/WNT13, WNT3, WNT3A, WNT5B, WNT6, WNT7B, WNT8A, WNT8B, WNT10A, WNT10B, WNT11, WNT14, and WNT14B/WNT15 by using bioinformatics, cDNA-library screening, and cDNA-PCR. Here, we investigated expression of human WNT5A mRNA in various normal tissues, 66 primary tumors derived from various tissues, and 15 human cancer cell lines. WNT5A mRNA was relatively highly expressed in salivary gland, bladder, uterus, placenta, and fetal kidney. Up-regulation of WNT5A mRNA was detected in 5 out of 8 cases of primary gastric cancer, 5 out of 18 cases of primary colorectal tumors, and in 2 out of 7 cases of primary uterus tumors by using matched tumor/normal expression array analysis. Up-regulation of WNT5A mRNA was also detected in 7 out of 10 other cases of primary gastric cancer by using cDNA-PCR. Although low-level expression of WNT5A mRNA was detected in gastric cancer cell line MKN45, WNT5A mRNA was almost undetectable in gastric cancer cell lines OKAJIMA, TMK1, MKN7, MKN28, MKN74, and KATO-III. Compared with frequent up-regulation of WNT5A mRNA in primary gastric cancer, expression levels of WNT5A mRNA in 7 gastric cancer cell lines were significantly lower than that in normal stomach. Frequent up-regulation of WNT5A mRNA in human primary gastric cancer might be due to cancer-stromal interaction.
authors.affiliation: Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tsukuji 5-chome, Chuo-ku, Tokyo 104-0045, Japan.
authors.forename: Tetsuroh
authors.initials: T
authors.lastname: Saitoh
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mine
authors.forename: Masaru
authors.initials: M
authors.lastname: Katoh
mesh_terms: D015342:DNA Probes; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D051153:Wnt Proteins; D000071818:Wnt-5a Protein
chemical_list: D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein