Aspergillus nidulans sod(VI)C1 mutation causes defects in cell wall biogenesis and protein secretion.
Growth at the restrictive temperature (42 degrees C) of Aspergillus nidulans B120, carrying the conditional-lethal mutation sod(VI)C1, was partially improved by the addition of 1.0 M sorbitol to the medium. The mutant grown at 42 degrees C, with osmotic stabilizer, showed abnormal hyphal morphology, a decrease in beta-1,3-glucan synthase activity as well as cell wall sugar content, but an increase in chitin synthase activity and N-acetyl-glucosamine content. The mutation also affected the secretion of extracellular protease. The temperature-dependent osmo-sensitive phenotype of a Saccharomyces cerevisiae alpha-COP mutation can be rescued by the A. nidulans sod(VI)C(+) gene. These results indicate that the sod(VI)C1 mutation affects proper processing of secretory proteins destined for the surface of cells or beyond.
Lee
Hwan Hee
HH
Department of Microbiology, College of Natural Sciences, Chungnam National University, Daejeon 305-764, South Korea.
Park
Jeong Seok
JS
Chae
Suhn Kee
SK
Maeng
Pil Jae
PJ
Park
Hee Moon
HM
D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002687:Chitin Synthase / Q000378:metabolism; D020756:Coatomer Protein / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal; D005936:Glucans / Q000096:biosynthesis; D009154:Mutation; D009995:Osmosis; D013012:Sorbitol / Q000494:pharmacology; D013696:Temperature; D047071:beta-Glucans
D020756:Coatomer Protein; D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; D013012:Sorbitol; C033363:beta-1,3-glucan; D002687:Chitin Synthase