Trehalose augments osteoprotegerin production in the FHs74Int human intestinal epithelial cell line.
The disaccharide trehalose has been shown to inhibit both bone loss in ovariectomized mice and excessive osteoclastogenesis in lipopolysaccharide-injected mice. However, the mechanism of osteoclastogenesis inhibition by oral administration of trehalose is still unclear. We report here for the first time that a human intestinal epithelial cell line, FHs74Int, also produces osteoprotegerin (OPG) and that trehalose augments OPG production by this cell line. Thus, these results suggest that trehalose promotes the production of OPG by intestinal epithelial cells, which then acts on bone marrow cells, resulting in the suppression of osteoclastogenesis.
Ariyasu
Toshio
T
Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., Okayama, Japan. fujisaki@hayashibara.co.jp
Arai
Chikako
C
Yoshizane
Chiyo
C
Kyono
Fumiyo
F
Hanaya
Toshiharu
T
Arai
Shigeyuki
S
Ikeda
Masao
M
Kurimoto
Masashi
M
D001483:Base Sequence; D017931:DNA Primers; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000187:drug effects*; D053244:Osteoprotegerin; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014199:Trehalose / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D006023:Glycoproteins; D053244:Osteoprotegerin; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D018124:Receptors, Tumor Necrosis Factor; C506030:TNFRSF11B protein, human; D014199:Trehalose