Expression of a membrane-anchored glycoprotein, the influenza virus hemagglutinin, by dicistronic replicons derived from the poliovirus genome.
Mono- and dicistronic poliovirus replicons were constructed to express the influenza virus hemagglutinin, retaining its signal peptide and transmembrane region. Picornavirus genomes do not normally encode glycoproteins, and only the dicistronic replicon, in which the foreign and poliovirus sequences were separated by the encephalomyocarditis virus internal ribosomal entry site, replicated and expressed glycosylated hemagglutinin.
Vignuzzi
Marco
M
Unité de Génétique Moléculaire des Virus Respiratoires, URA 1966 CNRS, Institut Pasteur, 75724 Paris Cédex 15, France.
Gerbaud
Sylvie
S
van der Werf
Sylvie
S
Escriou
Nicolas
N
D003001:Cloning, Molecular; D005822:Genetic Vectors; D006367:HeLa Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D009975:Orthomyxoviridae / Q000276:immunology / Q000378:metabolism*; D017955:Poliovirus / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D012093:Replicon; D014162:Transfection
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins