Synthesis and immunofluorescence assay of a new biotinylated paclitaxel.
7-(5'-Biotinylamidopropanoyl)paclitaxel was synthesised by chemical methods; its immunofluorescence assay and the cell uptake experiments were performed by use of human leukemia U937 cells. The results indicate that paclitaxel is arresting cell cycle at the G(2)M phase only.
Sambaiah
Thota
T
Department of Chemistry, Organosilicon and Synthesis Laboratory, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC.
King
Ke Yung
KY
Tsay
Shwu Chen
SC
Mei
Nai Wen
NW
Hakimclahi
Sharcon
S
Lai
Yiu Kay
YK
Lieu
Chien Hui
CH
Hwu
Jih Ru
JR
D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000378:metabolism*; D043823:Taxoids; D014407:Tumor Cells, Cultured; D020298:U937 Cells
C464531:7-(5'-Biotinylamidopropanoyl)paclitaxel; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D043823:Taxoids; D017239:Paclitaxel