An intronic AP-1 sequence mediates the transcriptional activation of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by serum.
The expression of F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is rapidly induced by growth factors. We report here that an AP-1 intragenic sequence located at position +612 of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase transcription initiation site is involved in the transcriptional activation of this gene by serum. We have demonstrated in vitro DNA-protein interaction on this AP-1 site of the F-promoter. Indeed, this element was recognized by c-Fos and JunD in vitro, and mutation or deletion of this element reduced the early response to serum stimulation by 60%. We conclude that the serum response of the F-type 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene requires the co-ordinated function of ets, E2F and AP-1 binding sites.
Joaquin
Manel
M
Departament de Bioquímica i Biologia Molecular, Div. IV, Facultat de Farmàcia, Universitat de Barcelona, Av. Diagonal 643, E-08028, Catalunya, Barcelona, Spain.
Salvadó
Cristina
C
Fernández de Mattos
Silvia
S
Tauler
Albert
A
D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D024202:Electrophoretic Mobility Shift Assay; D007438:Introns; D025481:Phosphofructokinase-2 / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D018808:Transcription Factor AP-1 / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
D018808:Transcription Factor AP-1; D025481:Phosphofructokinase-2