[Role of the pS(2) in gastric mucosa adaptative cytoprotection from stress].
OBJECTIVE
To determine the expression of pS(2) (a member of trefoil peptides) in gastric mucosal of rats undergone WRS, and to probe the role of pS(2) in adaptive cytoprotection.


METHODS
Wistar rats were exposed to single or repeated WRS for 4 h every other day for up to 6 days. Gastric mucosal blood flow (GMBF) was measured by LDF-3 Flowmeter. The degree of the gastric mucosal lesions (UI) was evaluated grossly and histologically. The expression of pS(2) was determined by RT-PCR and immunohistochemistry.


RESULTS
(1) WRS applied once produced numerous gastric mucosal erosions. UI gradually declined and GMBF restored on 3 d, 5 d after stress. UI was reduced to 20.8% and GMBF increased up to 94.5% of normal value. The expression of pS(2) was increased during the healing of stress-induced ulceration. The same results were observed by immunohistochemistry (0.50 +/- 0.13 vs 0.70 +/- 0.11, P < 0.01). (2) With repeated WRS, the adaptative cytoprotection against stress was developed. UI after four consecutive WRS was 22% of UI after one WRS. GMBF after four consecutive was 94.2% of normal value. Cell proliferation in the neck regions of gastric glands was activated. The expression of pS(2) was increased by using RT-PCR (0.37 +/- 0.02 vs 0.77 +/- 0.01, P < 0.01) and immunohistochemistry (0.55 +/- 0.04 vs 2.46 +/- 0.08, P < 0.01).


CONCLUSIONS
Increased expression of pS(2) could play an important role in adaptation of gastric mucose developed after repeated WRS.
Nie
Shinan
S
Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Center of Gastroenterology and Key Laboratory of PLA, Shanghai 200433, China.
Li
Zhaoshen
Z
Zhan
Xianbao
X
Tu
Zhenxing
Z
Xu
Guoming
G
Gong
Yanfang
Y
Man
Xiaohua
X
D000222:Adaptation, Physiological; D000818:Animals; D019610:Cytoprotection / Q000502:physiology*; D005753:Gastric Mucosa / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins