title: Potential viral pathogenic mechanism for new variant inflammatory bowel disease.
abstract: OBJECTIVE
  A new form of inflammatory bowel disease (ileocolonic lymphonodular hyperplasia) has been described in a cohort of children with developmental disorder. This study investigates the presence of persistent measles virus in the intestinal tissue of these patients (new variant inflammatory bowel disease) and a series of controls by molecular analysis.
  
  
  METHODS
  Formalin fixed, paraffin wax embedded and fresh frozen biopsies from the terminal ileum were examined from affected children and histological normal controls. The measles virus Fusion (F) and Haemagglutinin (H) genes were detected by TaqMan reverse transcription polymerase chain reaction (RT-PCR) and the Nucleocapsid (N) gene by RT in situ PCR. Localisation of the mRNA signal was performed using a specific follicular dendritic cell antibody.
  
  
  RESULTS
  Seventy five of 91 patients with a histologically confirmed diagnosis of ileal lymphonodular hyperplasia and enterocolitis were positive for measles virus in their intestinal tissue compared with five of 70 control patients. Measles virus was identified within the follicular dendritic cells and some lymphocytes in foci of reactive follicular hyperplasia. The copy number of measles virus ranged from one to 300,00 copies/ng total RNA.
  
  
  CONCLUSIONS
  The data confirm an association between the presence of measles virus and gut pathology in children with developmental disorder.
authors.affiliation: Department of Pathology, Coombe Women's Hospital, Dublin 8, Ireland.
authors.forename: V
authors.initials: V
authors.lastname: Uhlmann
authors.forename: C M
authors.initials: CM
authors.lastname: Martin
authors.forename: O
authors.initials: O
authors.lastname: Sheils
authors.forename: L
authors.initials: L
authors.lastname: Pilkington
authors.forename: I
authors.initials: I
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Killalea
authors.forename: S B
authors.initials: SB
authors.lastname: Murch
authors.forename: J
authors.initials: J
authors.lastname: Walker-Smith
authors.forename: M
authors.initials: M
authors.lastname: Thomson
authors.forename: A J
authors.initials: AJ
authors.lastname: Wakefield
authors.forename: J J
authors.initials: JJ
authors.lastname: O'Leary
mesh_terms: D000293:Adolescent; D001321:Autistic Disorder / Q000276:immunology / Q000821:virology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003106:Colon / Q000276:immunology; D003713:Dendritic Cells / Q000821:virology; D005260:Female; D006801:Humans; D006965:Hyperplasia; D007082:Ileum / Q000276:immunology; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000821:virology*; D008214:Lymphocytes / Q000821:virology; D008221:Lymphoid Tissue / Q000821:virology*; D008297:Male; D008459:Measles virus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017678:Sex Distribution
chemical_list: D012367:RNA, Viral