Effects of extremely low frequency (50 Hz) magnetic field on morphological and biochemical properties of human keratinocytes.
We investigated the effects on human keratinocytes (HaCaT) of exposure to a sinusoidal magnetic field of 2 mT (50 Hz). These cells are a good model for studying interaction of nonionising radiation, because they are not shielded from fields in vivo and also because they are resistant to both mechanical and thermal stimuli. We performed scanning microscopy which showed modification in shape and morphology in exposed cells. This modification is related to differential actin distribution as revealed by phalloidin fluorescence analysis. Moreover, the exposed cells show increased clonogenic capacity, as well as increased cellular growth as showed by clonogenicity assays and growth curves. Indirect immunofluorescence analysis using a fluorescent antibody against involucrin and beta4 integrin, which are respectively differentiation and adhesion markers, revealed an increase of involucrin expression and segregation of beta4 integrin in the cell membrane in cells exposed to 50 Hz; a higher percentage of the exposed cells shows a modified pattern of adhesion and differentiation markers. We also present evidence that exposure of HaCaT cells can interfere with protein kinase activity. Our observations confirm the hypothesis that electromagnetic fields at 50 Hz may modify cell membrane morphology and interfere with initiation of the signal cascade pathway and cellular adhesion.
Manni
Vanessa
V
Istituto di Neurobiologia e Medicina Molecolare CNR-Rome, Italy.
Lisi
Antonella
A
Pozzi
Deleana
D
Rieti
Sabrina
S
Serafino
Annalucia
A
Giuliani
Livio
L
Grimaldi
Settimio
S
D015703:Antigens, CD / Q000096:biosynthesis / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D006801:Humans; D039663:Integrin beta4; D053547:Keratin-14; D015603:Keratinocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects* / Q000648:ultrastructure; D007633:Keratins / Q000096:biosynthesis / Q000528:radiation effects; D007700:Kinetics; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010766:Phosphorylation / Q000528:radiation effects
D015703:Antigens, CD; D039663:Integrin beta4; C507540:KRT14 protein, human; D053547:Keratin-14; D007633:Keratins