Genetic and physical mapping, expression analysis and partial sequence of porcine PER1.
The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.
Skinner
T M
TM
Roslin Institute, Roslin, Midlothian, UK. tom.skinner@bbsrc.ac.uk
Lopez-Corrales
N L
NL
Anderson
S I
SI
Loudon
A S
AS
Lowden
S
S
Haley
C S
CS
Archibald
A L
AL
D000818:Animals; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*; D026801:Synteny
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger