Rapid action of cortisol and testosterone on lipogenic enzymes in a fresh water fish Oreochromis mossambicus: short-term in vivo and in vitro study.
Rapid action of steroid hormones on lipid metabolism is not reported so far in any vertebrate. The present study was intended to evaluate the quick actions of cortisol and testosterone on enzymes, namely malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH), and isocitrate dehydrogenase (ICDH) in Oreochromis mossambicus. Cortisol and testosterone produced rapid and opposite effects on the lipogenic enzymes studied. Cortisol significantly decreased the activities of ME, G6PDH, as early as 5 min and ICDH as early as 10 min in vitro (10(-6) M), and 30 min in vivo (0.1 microg/g body wt.) whereas the same doses of testosterone significantly stimulated the activity of all enzymes as early as 5 min in vitro and 30 min in vivo. Actinomycin D treatment did not interfere with the inhibiting effect of cortisol on enzyme activities when measured at 10 min in the in vitro system. The transcriptional inhibitor appeared to partially block the effect of cortisol in vivo. The stimulatory effect of testosterone was insensitive to the action of actinomycin D both in vivo and in vitro. These effects appear to be brought about independently of new protein synthesis because the rapid responses occurred within a latent period of 5-30 min and were insensitive to the action of actinomycin D, suggesting a non-genomic action.
Sunny
Francis
F
Endocrinology and Biochemistry Division, Department of Zoology, University of Kerala, Kariavattom, Trivandrum 695 581, Kerala, India.
Lakshmy
P S
PS
Oommen
Oommen V
OV
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology*; D017210:Tilapia
D011500:Protein Synthesis Inhibitors; D003609:Dactinomycin; D013739:Testosterone; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006854:Hydrocortisone