title: Primed B cells present type-II collagen to T cells.
abstract: Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further conclude that B cells have the potential to play an important role as APC in the development of CIA.
authors.affiliation: Section for Medical Inflammation Research, Lund University, Lund, Sweden. meirav.holmdahl@inflam.lu.se
authors.forename: M
authors.initials: M
authors.lastname: Holmdahl
authors.forename: M
authors.initials: M
authors.lastname: Vestberg
authors.forename: R
authors.initials: R
authors.lastname: Holmdahl
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001168:Arthritis / Q000209:etiology*; D001402:B-Lymphocytes / Q000502:physiology*; D024043:Collagen Type II / Q000276:immunology*; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D024043:Collagen Type II