[NF-kappa B expression in cholangiocarcinoma transfected with hepatitis C virus core gene].
OBJECTIVE
To study the role of hepatitis C virus (HCV) in the development of cholangiocarcinoma.


METHODS
Recombinant plasmid of HCV-C gene constructed by molecular cloning technique was identified with restricting enzyme map. Then, it was transfected into QBC939 cells with lipofectin. After selection with G418, the resistant colonies were obtained and analysed by immunocytochemistry and Western blotting. Their morphology was observed by transmission electron microscopy (TEM). The expression of NF-kappa B was detected by immunocytochemistry.


RESULTS
The results suggested that the recombinant plasmid was proved to carry the target gene by restricting enzyme map. Moreover, it could express HCV-C protein efficiently in QBC939 cells. The HCV-like particles were found in the cytoplasm by TEM, which were spherical with diameter of 50-80 nm possessing an outer membrane. Moreover, NF-kappa B activation was shown in HCV core gene-transfected cells.


CONCLUSIONS
Because HCV-C gene could express steadily in cholangiocarcinoma cells, the transfected tumor cells (QBC939-HCVc) are an experimental model for studying the effect of HCV on the development of cholangiocarcinoma. The activation of NF-kappa B may be related to escape from immune surveillance and carcinogenesis of cholangiocarcinoma.
Liu
Xiaofang
X
Department of General Surgery, Tongji Medical College Hospital, Central China University of Technology, Wuhan 430030, China.
Zou
Shengquan
S
Qiu
Fazu
F
D018281:Cholangiocarcinoma / Q000235:genetics* / Q000821:virology; D015870:Gene Expression; D016174:Hepacivirus / Q000235:genetics; D006801:Humans; D016328:NF-kappa B / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000821:virology; D014758:Viral Core Proteins / Q000235:genetics / Q000494:pharmacology*
D016328:NF-kappa B; D014758:Viral Core Proteins