A role for skin gammadelta T cells in wound repair.
Gammadelta T cell receptor-bearing dendritic epidermal T cells (DETCs) found in murine skin recognize antigen expressed by damaged or stressed keratinocytes. Activated DETCs produce keratinocyte growth factors (KGFs) and chemokines, raising the possibility that DETCs play a role in tissue repair. We performed wound healing studies and found defects in keratinocyte proliferation and tissue reepithelialization in the absence of wild-type DETCs. In vitro skin organ culture studies demonstrated that adding DETCs or recombinant KGF restored normal wound healing in gammadelta DETC-deficient skin. We propose that DETCs recognize antigen expressed by injured keratinocytes and produce factors that directly affect wound repair.
Jameson
Julie
J
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Ugarte
Karen
K
Chen
Nicole
N
Yachi
Pia
P
Fuchs
Elaine
E
Boismenu
Richard
R
Havran
Wendy L
WL
D000818:Animals; D002455:Cell Division; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015603:Keratinocytes / Q000502:physiology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D012867:Skin / Q000293:injuries; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D014945:Wound Healing
D016207:Cytokines; C496454:Fgf7 protein, mouse; D051526:Fibroblast Growth Factor 10; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors