[Inhibition of ERK1/2 activity and   c-fos   mRNA after coronary   artery balloon injury by intracoronary radiation in swine].
The effect of intracoronary radiation on extracellular signal regulated kinase 1/2 (ERK1/2) activity and c-fos mRNA after coronary artery balloon injury was investigated in swine. Twenty three swines were randomly divided into a radiation group and a control group after coronary balloon over stretch. The dilated segments in the radiation group were exposed to a dose of 20 Gy by a catheter based radiation system. The animals were sacrificed at 3 (6 swines from each group) and 30 days (6 swines from radiation group and 5 from control group) after the operation. The injured segments were processed to examine c-fos gene expression by reverse transcription polymerase chain reaction (RT PCR) and to measure the activity of ERK1/2 biochemically. Intracoronary radiation decreased significantly the ERK1/2 activity and gene expression of c-fos in the radiation treated animals 3 days after coronary balloon injury (20.5%,P<0.01; 47.7%,P<0.05), but neither ERK1/2 activity nor c-fos gene expression was significantly affected by endovascular radiation in animals 30 days after balloon injury. Therefore, both ERK1/2 and c-fos may be involved in inhibiting restenosis.
He
K L
KL
Department of Cardiology, The General Hospital of PLA, Beijing 100853, China. kl.he@bj.col.com.cn
Gai
L Y
LY
Huang
D X
DX
Liu
N K
NK
Tang
C S
CS
D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects; D000818:Animals; D001918:Brachytherapy / Q000379:methods*; D003131:Combined Modality Therapy; D023903:Coronary Restenosis / Q000517:prevention & control / Q000532:radiotherapy*; D005260:Female; D007496:Iridium Radioisotopes / Q000008:administration & dosage; D008297:Male; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D013556:Swine, Miniature
D007496:Iridium Radioisotopes; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases