Differential display of protein tyrosine kinase cDNAs from human fetal and adult brains.
Here we describe a differential display method for surveying the expression of most protein tyrosine kinases and applying it to cDNAs from human fetal and adult brains. The method involves two selective steps for processing the mRNA. At each step, degenerate oligonucleotide primers derived from highly conserved regions of the catalytic domain of the kinases are used. In the display with BstYI and BsiHKI digests of the cDNA, 65% and 59% of a total of 72 and 63 bands, respectively, represented fragments from a total of 27 different tyrosine kinases. The expression levels of the kinases in the display were comparable with those measured by RT-PCR. This method offers a relatively specific way to display differentially expressed gene families in any tissue and cell type.
Okada
M
M
Tokyo Medical and Dental University, Japan.
Yamaga
S
S
Yasuda
S
S
Weissman
S M
SM
Yasukochi
Y
Y
D000328:Adult; D001921:Brain / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis*; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics*
D018076:DNA, Complementary; D011505:Protein-Tyrosine Kinases