Evidence for the nitration of gamma-tocopherol in vivo: 5-nitro-gamma-tocopherol is elevated in the plasma of subjects with coronary heart disease.
This study investigated the hypothesis that nitration of gamma-tocopherol may be an important mechanism for the detoxification of reactive nitrogen oxide species in vivo. Using liquid chromatography-tandem MS we have shown that gamma-tocopherol can be nitrated in vivo to form 5-nitro-gamma-tocopherol and that concentrations of this compound are elevated in the plasma of subjects with coronary heart disease. In addition, we demonstrate in carotid-artery atherosclerotic plaque that nitration of gamma-tocopherol is also evident at levels similar to that seen in the plasma of subjects with coronary heart disease.
Morton
Lincoln W
LW
Department of Medicine, University of Western Australia and Western Australian Institute for Medical Research, PO Box X2213, GPO Perth, Western Australia, 6001, Australia.
Ward
Natalie C
NC
Croft
Kevin D
KD
Puddey
Ian B
IB
D015415:Biomarkers / Q000097:blood; D002340:Carotid Artery Diseases / Q000097:blood; D003327:Coronary Disease / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry; D012016:Reference Values; D024505:Tocopherols / Q000097:blood; D024504:gamma-Tocopherol / Q000031:analogs & derivatives* / Q000097:blood* / Q000138:chemical synthesis
C406664:5-nitro-gamma-tocopherol; D015415:Biomarkers; D024504:gamma-Tocopherol; D024505:Tocopherols