Production of a monoclonal antibody against Aeromonas hydrophila and its application to bacterial identification.
OBJECTIVE
to develop a monoclonal antibody (MAb) for the rapid detection of Aeromonas hydrophila in human faeces.


RESULTS
A monoclonal antibody with strong specificity against Aer. hydrophila was obtained by the fusion of myeloma cells and splenocytes of a mouse immunized with vegetative cells of Aer. hydrophila ATCC 7966, followed by a two-step selection against other species of the genera. ELISA analyses revealed that MAb 5F3 strongly reacts with all the Aer. hydrophila strains evaluated, showing a just basal reactivity against other species of the genera, especially Aer. sobria and Aer. caviae.


CONCLUSIONS
MAb 5F3 was characterized as an IgG that recognized a polypeptide of approximately 110 kDa.


CONCLUSIONS
This MAb could be used to detect Aer. hydrophila in human stool samples.
Delamare
A P L
AP
Institute of Biotechnology, University of Caxias do Sul, RS, Brazil.
Echeverrigaray
S
S
Duarte
K R
KR
Gomes
L H
LH
Costa
S O P
SO
D016980:Aeromonas hydrophila / Q000145:classification* / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D006825:Hybridomas; D051379:Mice
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal