title: Species relationships between antifungal chitinase and nuclear rDNA (internal transcribed spacer) sequences in the genus Hordeum.
abstract: The sequences of the chitinase gene (Chi-26) and the internal transcribed spacer of 18S - 5.8S - 26S rDNA (ITS1) were determined to analyze the phylogenetic relationships among species representing the four basic genomes of the genus Hordeum. Grouping analysis based on data for Chi-26 gene sequences placed Hordeum secalinum (H genome) near the Hordeum murinum complex (Xu genome), and Hordeum bulbosum distant from the other species that carried the I genome. ITS sequence data showed the expected grouping based on the genome classification of the species studied. Different sequences of ITS were detected even in the genomes of the diploid species. The results are interpreted in terms of defective or unfinished concerted evolution processes in each taxon.
authors.affiliation: Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid, Spain.
authors.forename: Bustos Alfredo
authors.initials: BA
authors.lastname: De
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Loarce
authors.forename: Nicolás
authors.initials: N
authors.lastname: Jouve
mesh_terms: D000935:Antifungal Agents; D001483:Base Sequence; D002688:Chitinases / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D004171:Diploidy; D019143:Evolution, Molecular; D017343:Genes, Plant; D014644:Genetic Variation; D018745:Genome, Plant; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014158:Transcription, Genetic
chemical_list: D000935:Antifungal Agents; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D002688:Chitinases