[Repair of porcine full-thickness skin defects with autologous tissue engineered skin].
OBJECTIVE
To explore a feasible method to repair full-thickness skin defects with tissue engineered techniques.


METHODS
The skin specimens were cut from the Changfeng hybrid swines' abdomen, then keratinocytes and fibroblasts were isolated and harvested by trypsin, EDTA and type II collagenase. The cells were seeded in petri dishes for primary culture. When the cells were in logarithmic growth phase, they were treated with dispase II (keratinocytes) or trypsin (fibroblasts) to separate them from the floor of the tissue culture dishes. A biodegradable material-pluronic F-127 was prefabricated and mixed with these cells, and then the cells-pluronic compounds were seeded evenly into polyglycolic acid (PGA). Tinally the constructs were replanted to autologous animals to repair full-thickness skin defects. Histological changes were observed in 1, 2, 4 and 8 weeks postsurgery.


RESULTS
The cells-pluronic F-127-PGA compounds could repair autologous full-thickness skin defects. Histologically, the tissue engineered skin was similar to normal skin with stratified epidermis overlying a moderately thick collageneous dermis.


CONCLUSIONS
Tissue engineered skin can repair autologous full-thickness skin defects with primary-cultured keratinocytes and fibroblasts as seed cells and PGA as a cell carrier.
Cao
Yilin
Y
Department of Plastic reconstructive Surgery, Shanghai Ninth People's Hospital, The Key Laboratory of Tissue Engineering, Shanghai Second Medical University, Shanghai 200011, China.
Cai
Xia
X
Cui
Lei
L
Shang
Qingxin
Q
Liu
Wei
W
Guan
Wenxiang
W
D000818:Animals; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D008297:Male; D011100:Polyglycolic Acid / Q000494:pharmacology; D016038:Skin Transplantation; D017768:Skin, Artificial; D013552:Swine; D023822:Tissue Engineering
D011100:Polyglycolic Acid