50 Hz magnetic fields of varying flux intensity affect cell shape changes in invertebrate immunocytes: the role of potassium ion channels.
The effect induced by exposure to 50 Hz magnetic fields (MFs) in immunocytes from the mussel Mytilus galloprovincialis is evaluated. The whole animal was exposed for 15 and 30 min to MF intensities ranging from 200 to 1,000 microT. The changes in the cellular shape of immunocytes, expressed as shape factor (SF), were studied at different times after addition of the chemotacting substance N-formyl-Meth-Leu-Phe (fMLP). Results show that MFs provoke differing delays in fMLP-induced cellular shape changes: 200 microT are ineffective, while levels from 300 microT upwards cause a significant increase in immunocyte SF values compared to controls. Reactivation of the cells is possible up to an intensity of 600 microT. The use of PCO 400, an opener of ATP-sensitive K+ channels, shows that potassium channels are involved in the effect of MFs on M. galloprovincialis immunocytes.
Ottaviani
Enzo
E
Department of Animal Biology, University of Modena and Reggio Emilia, Italy. ottaviani.enzo@unimo.it
Malagoli
Davide
D
Ferrari
Alex
A
Tagliazucchi
Davide
D
Conte
Angela
A
Gobba
Fabriziomaria
F
D000704:Analysis of Variance; D000818:Animals; D049872:Bivalvia / Q000528:radiation effects*; D048429:Cell Size / Q000528:radiation effects*; D004574:Electromagnetic Fields; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects
D015221:Potassium Channels; D009240:N-Formylmethionine Leucyl-Phenylalanine