Comparative studies on antigenicity and allergenicity of native and denatured egg white proteins.
The binding activities of IgG and IgE antibodies from egg-allergic patients to physically or chemically treated egg white proteins were examined and compared with those of rabbit anti-egg white IgG antibodies. The sera from eight patients and four rabbit antibodies were used in this study. The binding activities of human IgG antibody to partially denatured ovotransferrin (Tf), ovalbumin (OA), and lysozyme (Lys) forms were increased, whereas carboxymethylation (RCM) and heat treatment caused a dramatic decrease in the antigenicity of Tf and ovomucoid (OVM). Tf and OVM were major immunogenic antigens for the rabbit IgG response. Urea also caused Tf to exhibit greater rabbit IgG binding activity. In contrast, human and rabbit antibodies did not react with ovomucin. Partially denatured Tf and Lys also induced strong IgE binding activities. The allergenicity of Tf, OVM, and Lys was decreased by RCM, whereas OA retained its binding capacity. These results suggested that anti-OA IgE recognizes more sequential epitopes and that anti-OVM and Lys antibodies recognize both conformational and sequential epitopes. Tf and OVM were dominant allergens for the IgE antibodies of anaphylaxis patients, whereas IgE from atopic patients bound more strongly with OA and OVM.
Mine
Yoshinori
Y
Department of Food Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada. ymine@uoguelph.ca
Zhang
Jie Wei
JW
D000485:Allergens / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002675:Child, Preschool; D002942:Circular Dichroism; D004527:Egg Proteins / Q000737:chemistry* / Q000276:immunology*; D004531:Eggs; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D011489:Protein Denaturation; D011852:Radioallergosorbent Test
D000485:Allergens; D000941:Antigens; D004527:Egg Proteins; D007074:Immunoglobulin G; D007073:Immunoglobulin E