Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse.
Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P<0.05).
Vega-Estrada
J
J
Biotechnology and Bioengineering Department, CINVESTAV-IPN. Av. Instituto Politécnico Nacional, San Pedro Zacatenco, México DF, Mexico.
Flores-Cotera
L B
LB
Santiago
A
A
Magaña-Plaza
I
I
Montes-Horcasitas
C
C
D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D018533:Biomass; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002482:Cellulose; D005285:Fermentation; D007218:Industrial Microbiology; D013379:Substrate Specificity; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis*
D002482:Cellulose; C027433:bagasse; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase