Nicotine administration decreases nitric oxide synthase expression in the hypothalamus of food-deprived rats.
Effect of nicotine on nitric oxide synthase (NOS) expression in various hypothalamic regions was investigated in rats via nicotineamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry. Sprague-Dawley rats were divided into the fed group, the fed and nicotine-treated group, the food-deprived group, and the food-deprived and nicotine-treated group. The fed groups received abundant food and water, while food was withheld from the food-deprived groups for 48 h. The nicotine-treated groups were injected with nicotine. Following food deprivation, enhanced NAPDH-d expression was detected in the paraventricular nucleus, ventromedial hypothalamic nucleus, and lateral hypothalamic area of the hypothalamus. Nicotine administration to the food-deprived rats resulted in decreased NADPH-d positivity. The present results indicate that nicotine administration is effective in limiting the enhancement in NOS expression following food restriction.
Jang
Mi-Hyeon
MH
Department of Physiology, College of Medicine, Kyung Hee University, 1 Hoigi-dong, Dongdaemun-gu, Seoul 130-701, South Korea.
Shin
Min-Chul
MC
Lim
Baek-Vin
BV
Chung
Joo-Ho
JH
Kang
Hyung-Sook
HS
Kang
Soon-Ah
SA
Choue
Ryo-Won
RW
Kim
Ee-Hwa
EH
Kim
Chang-Ju
CJ
D000818:Animals; D001069:Appetite Regulation / Q000187:drug effects / Q000502:physiology; D005508:Food Deprivation / Q000502:physiology*; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D009538:Nicotine / Q000494:pharmacology*; D026602:Nitrergic Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000187:drug effects* / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014029:Tobacco Use Disorder / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects* / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D009569:Nitric Oxide; D009538:Nicotine; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase