Marker-free transformation: increasing transformation frequency by the use of regeneration-promoting genes.
The generation of transgenic plants free of antibiotic resistance markers is a major challenge to plant biologists and plant breeders. Currently, there are two main strategies to achieve this goal: one approach is to excise or segregate marker genes from the host genome after regeneration of transgenic plants, and the second is based on so-called 'marker-free' transformation. Marker-free transformation has been successfully demonstrated by the use of several plant and non-plant genes that are capable of promoting explant regeneration. This approach appears not only to be effective for the generation of marker-free transgenic plants, but also has great potential to improve the transformation frequency of recalcitrant species.
Zuo
Jianru
J
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 917 Datun Road, Beijing 100101, China.
Niu
Qi-Wen
QW
Ikeda
Yoshihisa
Y
Chua
Nam-Hai
NH
D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics*; D012038:Regeneration / Q000235:genetics*; D014170:Transformation, Genetic / Q000502:physiology*; D019076:Transgenes
D005819:Genetic Markers