Enhancement of the conversion of toluene by Pseudomonas putida F-1 using organic cosolvents.
Pseudomonas putida F-1 (ATCC700007) was used as a model organism in stirred tank reactors to study conversion enhancement of poorly soluble substrates by organic cosolvents. After a literature study, silicone oil was used as a solvent system to enhance the mass transfer rate. To study the benefits of the organic solvent addition, batch experiments were conducted in two side-by-side fermentation vessels (experimental and control) at three different levels of silicone oil (10, 30, and 50%). Results showed that the presence of silicone oil resulted in a 100% increase in the toluene mass transfer compared to the control. Experiments in continuous stirred-tank reactors showed that improved conversion could be obtained at higher agitation rates.
Rodriguez
M
M
Bioprocessing Research and Development Center, Oak Ridge National Laboratory, TN 37831-6226, USA.
Klasson
K T
KT
Davison
B H
BH
D019149:Bioreactors; D005285:Fermentation; D005740:Gases; D007700:Kinetics; D016958:Pseudomonas putida / Q000378:metabolism*; D012827:Silicone Oils; D012997:Solvents; D014050:Toluene / Q000378:metabolism*
D005740:Gases; D012827:Silicone Oils; D012997:Solvents; D014050:Toluene