Regulation of gene expression by low levels of ultraviolet-B radiation in Pisum sativum: isolation of novel genes by suppression subtractive hybridisation.
Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-B(BE,300) 0.13 W m(-2)) of ultraviolet-B radiation (UV-B; 290-320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these two (PsTSDC and PsUOS1) were increased and depressed by UV-B treatment, respectively. Domains in the PsTSDC translation product was similar to TIR (Toll-Interleukin-1 receptor-similar) domains and a NB-ARC domain (nucleotide-binding domain in APAF-1, R gene products and CED-4). The PsUOS1 translation product was similar to an open reading frame in Arabidopsis. Genes encoding embryo-abundant protein (PsEMB) and S-adenosyl-L-methionine synthase (PsSAMS) were induced by UV-B, whereas the transcript levels for genes encoding sucrose transport protein (PsSUT) or ribulose-5-phosphate 3-epimerase (PsR5P3E) were decreased. These regulation patterns are novel, and the PsEMB and PsR5P3E sequences are reported for the first time. The stress-specificity of regulation of these genes were tested by ozone fumigation (100 ppb O(3)). Qualitatively, the similarity of expression after both UV-B and ozone exposure suggests that, for these genes, similar stress-response pathways are in action.
Sävenstrand
Helena
H
Biochemistry and Biophysics, Department of Chemistry, Göteborg University, P.O. Box 462, S-405 30, Sweden.
Brosché
Mikael
M
Strid
Ake
A
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D015152:Blotting, Northern; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010126:Ozone / Q000494:pharmacology; D018532:Pisum sativum / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D026901:Membrane Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; C078729:sucrose transport protein, plant; D010126:Ozone; C111241:methionine synthase reductase; D005287:Ferredoxin-NADP Reductase; D002238:Carbohydrate Epimerases; C022553:ribulosephosphate 3-epimerase