title: Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
abstract: Mutations in the Artemis protein in humans result in hypersensitivity to DNA double-strand break-inducing agents and absence of B and T lymphocytes (radiosensitive severe combined immune deficiency [RS-SCID]). Here, we report that Artemis forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs) in the absence of DNA. The purified Artemis protein alone possesses single-strand-specific 5' to 3' exonuclease activity. Upon complex formation, DNA-PKcs phosphorylates Artemis, and Artemis acquires endonucleolytic activity on 5' and 3' overhangs, as well as hairpins. Finally, the Artemis:DNA-PKcs complex can open hairpins generated by the RAG complex. Thus, DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in nonhomologous DNA end joining.
authors.affiliation: Norris Comprehensive Cancer Center, Rm. 5428, Departments of Biochemistry & Molecular Biology, Pathology, Biological Sciences, and Molecular Microbiology & Immunology, 1441 Eastlake Avenue, Los Angeles, CA 90033, USA.
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Ma
authors.forename: Ulrich
authors.initials: U
authors.lastname: Pannicke
authors.forename: Klaus
authors.initials: K
authors.lastname: Schwarz
authors.forename: Michael R
authors.initials: MR
authors.lastname: Lieber
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D004789:Enzyme Activation; D024243:HMGB1 Protein / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic / Q000502:physiology*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D024243:HMGB1 Protein; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D000255:Adenosine Triphosphate; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; C426344:DCLRE1C protein, human; D004720:Endonucleases; D001618:beta-Lactamases