title: Cloning of a prolidase gene from Aspergillus nidulans and characterisation of its product.
abstract: Using EST sequence information available from the filamentous fungus Aspergillus nidulans as a starting point, we have cloned the prolidase-encoding gene, designated pepP. Introduction of multiple copies of this gene into the A. nidulansgenome leads to overexpression of an intracellular prolidase activity. Prolidase was subsequently purified and characterised from an overexpressing strain. The enzyme activity is dependent on manganese as a cofactor, is specific for dipeptides and hydrolyses only dipeptides with a C-terminal proline residue. Although these proline dipeptides are released both intracellularly and extracellularly, prolidase activity was detected only intracellularly.
authors.affiliation: Laboratory of Microbiology, Wageningen University, Dreijenlaan 2, NL-6703 HA, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Jalving
authors.forename: P
authors.initials: P
authors.lastname: Bron
authors.forename: H C M
authors.initials: HC
authors.lastname: Kester
authors.forename: J
authors.initials: J
authors.lastname: Visser
authors.forename: P J
authors.initials: PJ
authors.lastname: Schaap
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004150:Dipeptidases / Q000235:genetics* / Q000378:metabolism*; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D005800:Genes, Fungal; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D013379:Substrate Specificity
chemical_list: D004271:DNA, Fungal; D004151:Dipeptides; D004150:Dipeptidases; C022449:proline dipeptidase