title: The Arabidopsis TONNEAU2 gene encodes a putative novel protein phosphatase 2A regulatory subunit essential for the control of the cortical cytoskeleton.
abstract: In Arabidopsis ton2 mutants, abnormalities of the cortical microtubular cytoskeleton, such as disorganization of the interphase microtubule array and lack of the preprophase band before mitosis, markedly affect cell shape and arrangement as well as overall plant morphology. We present the molecular isolation of the TON2 gene, which is highly conserved in higher plants and has a vertebrate homolog of unknown function. It encodes a protein similar in its C-terminal part to B" regulatory subunits of type 2A protein phosphatases (PP2As). We show that the TON2 protein interacts with an Arabidopsis type A subunit of PP2A in the yeast two-hybrid system and thus likely defines a novel subclass of PP2A subunits that are possibly involved in the control of cytoskeletal structures in plants.
authors.affiliation: Station de Génétique et Amélioration des Plantes, Centre de Versailles, F78026 Versailles Cedex, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Camilleri
authors.forename: Juliette
authors.initials: J
authors.lastname: Azimzadeh
authors.forename: Martine
authors.initials: M
authors.lastname: Pastuglia
authors.forename: Catherine
authors.initials: C
authors.lastname: Bellini
authors.forename: Olivier
authors.initials: O
authors.lastname: Grandjean
authors.forename: David
authors.initials: D
authors.lastname: Bouchez
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D054648:Protein Phosphatase 2; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D003598:Cytoskeletal Proteins; C460109:FASS protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2