title: 2'-hydroxypyridoxol, a biosynthetic precursor of vitamins B(6) and B(1) in yeast.
abstract: In separate experiments cultures of the yeast Saccharomyces cerevisiae ATTC 7752 were grown in the presence of [5',5'-2H2]- or of [2',2',5',5'-2H4]-3-hydroxy-2,4,5-tri(hydroxymethyl)pyridine (i.e., 2'-hydroxypyridoxol). The 2H NMR spectra of the samples of pyridoxamine dihydrochloride and of thiamin chloride hydrochloride that were isolated from the two experiments showed the presence of deuterium at the corresponding sites. Entry of deuterium from the specifically 2H-labeled samples of 2'-hydroxypyridoxol into the predicted sites of pyridoxamine and of the pyrimidine unit of thiamin provides the first unequivocal evidence that, in yeast, 2'-hydroxypyridoxol is an intermediate on the route from a C5-sugar into vitamin B6, and adds to the evidence that pyridoxol serves as a precursor of the pyrimidine unit of thiamin, supplying the C5N unit, C-2',2,N-1,C-6,5,5' as an intact unit.
authors.affiliation: Department of Chemistry, McMaster University, Hamilton, Ontario, Canada L8S 4M1.
authors.forename: Johannes
authors.initials: J
authors.lastname: Zeidler
authors.forename: Nisar
authors.initials: N
authors.lastname: Ullah
authors.forename: Ram Nath
authors.initials: RN
authors.lastname: Gupta
authors.forename: Richard M
authors.initials: RM
authors.lastname: Pauloski
authors.forename: Brian G
authors.initials: BG
authors.lastname: Sayer
authors.forename: Ian D
authors.initials: ID
authors.lastname: Spenser
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular; D011736:Pyridoxine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*; D025101:Vitamin B 6 / Q000096:biosynthesis*
chemical_list: C459723:2'-hydroxypyridoxol; D025101:Vitamin B 6; D011736:Pyridoxine; D013831:Thiamine