Der f 16: a novel gelsolin-related molecule identified as an allergen from the house dust mite, Dermatophagoides farinae.
Allergen from the house dust mite (Dermatophagoides sp.) is a major trigger factor of allergic disorders, and its characterization is crucial for the development of specific diagnosis or immunotherapy. Here we report the identification of a novel dust mite (Dermatophagoides farinae) antigen whose primary structure belongs to the gelsolin family, a group of actin cytoskeleton-regulatory proteins. Isolated mite cDNA, termed Der f 16, encodes 480 amino acids comprising a four-repeated gelsolin-like segmental structure, which is not seen in conventional gelsolin family members. Enzyme immunoassay indicated that recombinant Der f 16 protein, prepared using an Escherichia coli expression system, bound IgE from mite-allergic patients at 47% (8/17) frequency. This is the first evidence that the gelsolin family represents a new class of allergen recognizable by atopic patient IgE.
Kawamoto
Seiji
S
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, 739-8530, Higashi-Hiroshima, Japan. skawa@hiroshima-u.ac.jp
Suzuki
Takayuki
T
Aki
Tsunehiro
T
Katsutani
Takashi
T
Tsuboi
Shinji
S
Shigeta
Seiko
S
Ono
Kazuhisa
K
D000485:Allergens / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018260:Gelsolin / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism; D008925:Mites / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D018260:Gelsolin; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E