Flow cytometric analysis of T cell receptor signal transduction.
Intracellular assays of signal transduction in T cells stimulated by antigen in vivo have been limited by requirements for large numbers of highly purified cells to perform conventional biochemical techniques. Here, we describe a technique using flow cytometry to study signaling events in T cells after antigen recognition in the body.
Zell
Traci
T
University of Minnesota, Department of Microbiology, Room 6-220, Basic Sciences and Biomedical Engineering, 312 Church Street, SE, Minneapolis, MN 55455, USA. zellx001@gold.tc.umn.edu
Jenkins
Marc K
MK
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D002645:Chickens; D005434:Flow Cytometry / Q000379:methods*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015398:Signal Transduction / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism* / Q000502:physiology / Q000637:transplantation
D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin