title: Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1.
abstract: We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.
authors.affiliation: Laboratory for Microbiology, Wageningen University, 6703CT Wageningen, The Netherlands. deward@unity.ncsu.edu
authors.forename: Donald E
authors.initials: DE
authors.lastname: Ward
authors.forename: Ingrid M
authors.initials: IM
authors.lastname: Revet
authors.forename: Renu
authors.initials: R
authors.lastname: Nandakumar
authors.forename: Jon H
authors.initials: JH
authors.lastname: Tuttle
authors.forename: Willem M
authors.initials: WM
authors.lastname: de Vos
authors.forename: John
authors.initials: J
authors.lastname: van der Oost
authors.forename: Jocelyne
authors.initials: J
authors.lastname: DiRuggiero
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D019711:Pyrococcus / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D004277:DNA, Single-Stranded