Synthesis of solid-supported mirror-image sugars: a novel method for selecting receptors for cellular-surface carbohydrates.
We introduced a novel method, through mirror-image phage display, for the identification of high-affinity D-peptides to target specific cell-surface carbohydrates. Both 3-deoxy-alpha-L-manno-2-octulosonic acid (L-KDO) and L-sialic acid and an L-sialo-disaccharide have been synthesized and attached to a solid support for selection of high-affinity peptide binders displayed on phages. Our initial studies in this effort produce single-chain Fab sequences and dodecapeptides that bind to sialic acid and KDO with nanomolar and high micromolar affinity.
Kozlov
I A
IA
Department of Chemistry, The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA.
Mao
S
S
Xu
Y
Y
Huang
X
X
Lee
L
L
Sears
P S
PS
Gao
C
C
Coyle
A R
AR
Janda
K D
KD
Wong
C H
CH
D000595:Amino Acid Sequence; D000915:Antibody Affinity; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D002846:Chromatography, Affinity; D007135:Immunoglobulin Variable Region / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D001704:Biopolymers; D002241:Carbohydrates; D007135:Immunoglobulin Variable Region; D011956:Receptors, Cell Surface