title: Molecular cloning, structure and expression of a novel nuclear RNA-binding cyclophilin-like gene (PPIL4) from human fetal brain.
abstract: The cyclophilins are members of a highly conserved, ubiquitous family, and play an important role in protein folding, immunosuppression by cyclosporin A (CsA), and infection of HIV-1 virions. Here we report that a novel member of the cyclophilin family, PPIL4, was cloned and identified during the large-scale sequencing analysis from a human fetal brain cDNA library. The PPIL4 gene encodes a protein which shares 96% amino acid identity with a protein encoded by a putative gene recently cloned from several different early stages of mouse embryo, and its homologues are found in several other organisms. According to bioinformatics analysis, the PPIL4 gene was found to be located in chromosome 6q24-->q25. Besides the PPIase motif, PPIL4 also has an RNA recognition motif (RRM), a pair of bipartite nuclear targeting sequences, and a lysine rich domain. RT-PCR analysis indicated that PPIL4 gene expression is abundant in kidney but has a ubiquitously low expression pattern in other human adult tissues.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Zeng
authors.forename: Z
authors.initials: Z
authors.lastname: Zhou
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Cheng
authors.forename: M
authors.initials: M
authors.lastname: Xu
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.forename: Y
authors.initials: Y
authors.lastname: Mao
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003001:Cloning, Molecular; D021983:Cyclophilins / Q000737:chemistry* / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D021983:Cyclophilins; C455828:PPIL4 protein, human