Evaluation of glucoiberin reference material from Iberis amara by spectroscopic fingerprinting.
Increasing worldwide regulations require increased efforts toward validation of analytical and pharmacological reference materials. A detailed survey of glucoiberin, a prototype lead constituent of therapeutic value, using 1D/2D NMR, MS, and X-ray spectroscopy provided precise phytochemical data for structure assignment. Quantitative reference validation was achieved by the recently proposed qNMR method.
Jaki
Birgit
B
Zentralinstitut Arzneimittelforschung (ZA) GmbH, Kranzweiherweg 10, 53489 Sinzig, Germany.
Sticher
Otto
O
Veit
Markus
M
Fröhlich
Roland
R
Pauli
Guido F
GF
D002794:Choline / Q000031:analogs & derivatives* / Q000737:chemistry; D018360:Crystallography, X-Ray; D005961:Glucosinolates / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000592:standards; D013058:Mass Spectrometry; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010946:Plants, Medicinal / Q000737:chemistry*; D012015:Reference Standards; D012639:Seeds / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D005961:Glucosinolates; C457341:glucoiberin; C010330:sinigrin; D002794:Choline; C003313:sinalbin