Bovine liver catalase is separated into several distinct bands by electrophoresis in a linear concentration gradient of polyacrylamide. Apparently, disc electrophoresis under these conditions leads to a series of enzymatic active forms of catalase. Their molecular weights are: 248,000; 295,000; 368,000; 486,000; 705,000 respectively. Density gradient centrifugation separates catalase into two components with molecular weights of 252,000 and 316,000. The observed differences in molecular weight distribution between gel-electrophoresis and density gradient centrifugation are discussed.
Diezel
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W
Physiologisch-chemisches Institut der Karl-Marx-Universität, Leipzig, DDR
Liebe
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S
Kopperschläger
G
G
Hofmann
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E