title: Large-scale isolation and crystallization of epothilone D from Myxococcus xanthus cultures.
abstract: The introduction of the epothilone polyketide synthase (PKS) into Myxococcus xanthus has enabled the heterologous production of epothilone D (1) on a large scale. To isolate this valuable product from the fermentation medium, an economical, scalable, and high-yielding purification process was developed. With the crystallization of 1 from a binary solvent system that consisted of ethanol and water, the product was recovered as white crystals with a final purity of > or =97% (w/w). This is the first reported crystallization of 1.
authors.affiliation: Kosan Biosciences, Inc., 3832 Bay Center Place, Hayward, California 94545, USA. arslanian@kosan.com
authors.forename: Robert L
authors.initials: RL
authors.lastname: Arslanian
authors.forename: Charles D
authors.initials: CD
authors.lastname: Parker
authors.forename: Peter K
authors.initials: PK
authors.lastname: Wang
authors.forename: James R
authors.initials: JR
authors.lastname: McIntire
authors.forename: Janice
authors.initials: J
authors.lastname: Lau
authors.forename: Courtney
authors.initials: C
authors.lastname: Starks
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Licari
mesh_terms: D002244:Carbon; D002625:Chemistry, Organic / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D034261:Epothilones; D004852:Epoxy Compounds / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008968:Molecular Conformation; D009097:Multienzyme Complexes / Q000378:metabolism*; D016940:Myxococcus / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification*
chemical_list: D034261:Epothilones; D004852:Epoxy Compounds; D009097:Multienzyme Complexes; D013844:Thiazoles; D002244:Carbon; C114026:desoxyepothilone B