Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci.
A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three additional E. faecium strains were also analyzed by I-CeuI digestion, and the genome sizes were found to vary from 2550 to 2995 kb. We further investigated the genome sizes and number of rrn operons in four E. faecalis, one E. avium, and one E. durans strains. The genome sizes were larger than E. faecium: 3000-3250 kb in E. faecalis, 3445 kb in E. avium, and 3070 kb in E. durans. E. avium and E. durans contained six rrn operons as in E. faecium, but all the E. faecalis strains possessed four rrn operons.
Oana
Kozue
K
Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Asahi 3-1-1, 390-8621, Matsumoto, Japan.
Okimura
Yukie
Y
Kawakami
Yoshiyuki
Y
Hayashida
Nobuaki
N
Shimosaka
Makoto
M
Okazaki
Mitsuo
M
Hayashi
Tetsuya
T
Ohnishi
Makoto
M
D002876:Chromosomes, Bacterial / Q000737:chemistry*; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific; D004706:Endodeoxyribonucleases; D016983:Enterococcus / Q000235:genetics*; D016984:Enterococcus faecium / Q000235:genetics*; D014644:Genetic Variation; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012407:rRNA Operon
D004706:Endodeoxyribonucleases; C071132:endodeoxyribonuclease I-CeuI; C066157:endodeoxyribonuclease Sse83871; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases