A neuronal receptor, neuropilin-1, is essential for the initiation of the primary immune response.
The initiation of a primary immune response requires contact between dendritic cells (DCs) and resting T cells. However, little is known about the proteins that mediate this initial contact. We show here that neuropilin-1, a receptor involved in axon guidance, was expressed by human DCs and resting T cells both in vitro and in vivo. The initial contact between DCs and resting T cells led to neuropilin-1 polarization on T cells. DCs and resting T cells specifically bound soluble neuropilin-1, and resting T cells formed clusters with neuropilin-1-transfected COS-7 cells in a neuropilin-1-dependent manner. Functionally, preincubation of DCs or resting T cells with blocking neuropilin-1 antibodies inhibited DC-induced proliferation of resting T cells. These data suggest that neuropilin-1 mediates interactions between DCs and T cells that are essential for initiation of the primary immune response and show parallels between the nervous and immune systems.
Tordjman
Rafaèle
R
Institut Cochin, Departement d'Hematologie, INSERM U567, CNRS UMR 8104, Paris, France. tordjman@cochin.inserm.fr
Lepelletier
Yves
Y
Lemarchandel
Valérie
V
Cambot
Marie
M
Gaulard
Philippe
P
Hermine
Olivier
O
Roméo
Paul-Henri
PH
D000818:Animals; D019556:COS Cells; D002450:Cell Communication / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002522:Chlorocebus aethiops; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000276:immunology; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D039942:Neuropilin-1; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D039942:Neuropilin-1