A miniature fiber optic surface plasmon resonance sensor for fast detection of Staphylococcal enterotoxin B.
A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min.
Slavík
R
R
Institute of Radio Engineering and Electronics, AS CR, Chaberská 57, 182 51, Prague, Czech Republic.
Homola
J
J
Brynda
E
E
D004768:Enterotoxins / Q000032:analysis*; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D008904:Miniaturization; D055100:Optical Fibers; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal