title: [Effect of cyclosporin A on the growth of human first trimester cytotrophoblasts in vitro].
abstract: OBJECTIVE
  To investigate effect of cyclosporin A (CsA) on the growth of first trimester human cytotrophoblast in vitro.
  
  
  METHODS
  To isolate and purificate first trimester human cytotrophoblasts by way of percoll density gradient centrifugation; to evaluate the effect of CsA on in vitro proliferation of cytotrophoblasts using (3)H-thymidine incorporation assay; to analyze of cell cycle of cytotrophoblasts with flow cytometric assay; and to survey morphology of the cells by scanning electro-microscope respectively.
  
  
  RESULTS
  CsA could increase in vitro proliferation of first trimester human cytotrophoblasts when concentration of CsA ranged from 1 x 10(-4) micromol/L to 1 micromol/L, but decrease in vitro proliferation of the cells if the concentration reached to 10 micromol/L. G(2)-M phase increased from (2.8 +/- 0.5)% to (6.7 +/- 1.3)% when the cytotrophoblasts were incubated with culture medium containing 1 micromol/L CsA for 24 hours, while S phase increased from (14 +/- 5)% to (28 +/- 8)% and apoptosis decreased from (8.7 +/- 2.2)% to (3.6 +/- 1.0)% when the culture in the same incubating condition lasted for 48 hours. Pseudopodia of the trophoblasts increased in number and length when they were incubated in culture medium containing 1 micromol/L of CsA for 48 hours, but decreased if the concentration reached to 10 micromol/L with the same incubating duration.
  
  
  CONCLUSIONS
  CsA is able to promote growth of first trimester human cytotrophoblasts and change their morphology, which appears to increase in invasive ability.
authors.affiliation: Laboratory of Reproductive Jmmunology, Institute of Obstetrics and Gynecology, Fudan University, Shanghai 200011, China.
authors.forename: Fengting
authors.initials: F
authors.lastname: Yan
authors.forename: Dajin
authors.initials: D
authors.lastname: Li
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Mingyan
authors.initials: M
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Meng
authors.forename: Jiang
authors.initials: J
authors.lastname: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D016196:S Phase / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects*
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine