Flow cytometry analysis of platelet cyclooxygenase-2 expression: induction of platelet cyclooxygenase-2 in patients undergoing coronary artery bypass grafting.
There are conflicting reports about the expression of cyclooxygenase (COX)-2 in human platelets. The present study describes a flow cytometric method for the measurement of platelet COX. Both COX-1 and COX-2 were shown to be expressed in platelets from patients undergoing a coronary artery bypass graft. There was a significant increase in COX-2 expression at day 5 as compared with pre-surgery values (mean fluorescence 12.31 +/- 0.88 versus 9.15 +/- 0.88; means +/- SEM, n = 7, P < 0.05), whereas COX-1 levels did not change (13.45 +/- 1.11 versus 12.38 +/- 1.41; n = 7, P > 0.05).
Weber
Artur-Aron
AA
Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Heinmrich-Heine-Universität, Moorenstrasse 5, D-40225 Düsseldorf, Germany.
Przytulski
Boris
B
Schumacher
Marc
M
Zimmermann
Norbert
N
Gams
Emmeran
E
Hohlfeld
Thomas
T
Schrör
Karsten
K
D001241:Aspirin / Q000378:metabolism; D001792:Blood Platelets / Q000201:enzymology*; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000201:enzymology* / Q000378:metabolism; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors / Q000378:metabolism; D004789:Enzyme Activation; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008565:Membrane Proteins; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*; D013997:Time Factors
D052246:Cyclooxygenase 2 Inhibitors; D016861:Cyclooxygenase Inhibitors; D007527:Isoenzymes; D008565:Membrane Proteins; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D001241:Aspirin