title: Adjuvant immunotherapy of resected, intermediate-thickness, node-negative melanoma with an allogeneic tumor vaccine: impact of HLA class I antigen expression on outcome.
abstract: OBJECTIVE
  An association between expression of > or = two of five HLA class I antigens (HLA-A2, HLA-A28, HLA-B44, HLA-B45, and HLA-C3; collectively called M5) and response to an allogeneic melanoma vaccine (Melacine; Corixa Corporation, Seattle, WA) has been described in stage IV melanoma. This study investigated whether class I antigen expression impacted relapse-free survival (RFS) after adjuvant therapy with this vaccine.
  
  
  METHODS
  We performed class I (HLA-A, HLA-B, and HLA-C) serotyping on patients enrolled onto Southwest Oncology Group Trial 9035, a randomized, observation-controlled, phase III trial of adjuvant Melacine. All patients had clinically node-negative cutaneous melanoma (1.5 to 4.0 mm). Interactions between treatment and class I antigen expression were tested. Analyses involved all serotyped patients and were adjusted for tumor thickness, method of nodal staging, sex, ulceration, and primary tumor site.
  
  
  RESULTS
  HLA typing was performed on 553 (80%) of the 689 enrolled patients (294 vaccinated and 259 observed). Expression of > or = two M5 antigens was associated with a superior vaccine treatment effect. Among patients who matched > or = two of the M5, the 97 vaccine-treated patients had improved RFS compared with the 78 observation patients (5-year relapse-free survival, 83% v 59%; P =.0002). The major components of this effect were contributed by HLA-A2 and HLA-C3. Among those who were HLA-A2-positive and/or HLA-C3-positive, the 5-year RFS for vaccinated patients was 77%, compared with 64% for observation (P =.004). There was no impact of HLA-A2 and/or HLA-C3 expression among observation patients.
  
  
  CONCLUSIONS
  This prospective analysis indicates a highly significant benefit of adjuvant therapy with Melacine among patients expressing > or = two of the M5 class I antigens, validating a prior observation in stage IV disease. HLA-A2 and HLA-C3 contributed most to this effect. Processed melanoma peptides found in Melacine may be presented by HLA-A2 and HLA-C3 and play a role in preventing relapse in vaccinated patients.
authors.affiliation: Vanderbilt University, Nashville, TN, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Sosman
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Unger
authors.forename: P-Y
authors.initials: PY
authors.lastname: Liu
authors.forename: Lawrence E
authors.initials: LE
authors.lastname: Flaherty
authors.forename: Min S
authors.initials: MS
authors.lastname: Park
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Kempf
authors.forename: John A
authors.initials: JA
authors.lastname: Thompson
authors.forename: Paul I
authors.initials: PI
authors.lastname: Terasaki
authors.forename: Vernon K
authors.initials: VK
authors.lastname: Sondak
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use*; D018572:Disease-Free Survival; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008297:Male; D008545:Melanoma / Q000276:immunology* / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D008875:Middle Aged; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology / Q000601:surgery / Q000628:therapy
chemical_list: D000276:Adjuvants, Immunologic; D019496:Cancer Vaccines; D015395:Histocompatibility Antigens Class I; C116434:Melacine