title: Nitric oxide does not inhibit cerebral cytochrome oxidase in vivo or in the reactive hyperemic phase after brief anoxia in the adult rat.
abstract: In this study, near-infrared spectroscopy was applied to examine whether cytochrome oxidase in the rat brain is inhibited by nitric oxide in vivo. During normoxia, intravenous N(G)-nitro-L-arginine methyl ester (L-NAME) administration significantly decreased the cerebral saturation of hemoglobin with oxygen but did not alter the cytochrome oxidase redox state. Anoxia significantly reduced the cytochrome oxidase. The time course of the recovery of the redox state during reoxygenation was not altered by L-NAME. The results suggest that in adult rats, cytochrome oxidase is not inhibited by nitric oxide, either in physiologic conditions or during reoxygenation after a brief anoxic period.
authors.affiliation: Department of Neuropathology, Discovery Research, Janssen Research Foundation, Turnhoutseweg 30, B-2340 Beerse, Belgium. gdvissch@janbe.jnj.com
authors.forename: Geofrey
authors.initials: G
authors.lastname: De Visscher
authors.forename: Roger
authors.initials: R
authors.lastname: Springett
authors.forename: David T
authors.initials: DT
authors.lastname: Delpy
authors.forename: Jos
authors.initials: J
authors.lastname: Van Reempts
authors.forename: Marcel
authors.initials: M
authors.lastname: Borgers
authors.forename: Koen
authors.initials: K
authors.lastname: van Rossem
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006454:Hemoglobins / Q000378:metabolism; D006940:Hyperemia / Q000201:enzymology*; D002534:Hypoxia, Brain / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010084:Oxidation-Reduction; D010100:Oxygen / Q000097:blood; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019265:Spectroscopy, Near-Infrared
chemical_list: D004791:Enzyme Inhibitors; D006454:Hemoglobins; D010108:Oxyhemoglobins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D003576:Electron Transport Complex IV; D010100:Oxygen; D019331:NG-Nitroarginine Methyl Ester