Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients.
By comparing the CSF proteome between Alzheimer disease (AD) patients and controls it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. We used mini-gel technology in a two-dimensional electrophoresis procedure, sensitive SYPRO Ruby staining and mass spectrometry for clinical screening of disease-influenced CSF proteins in 15 AD patients and 12 controls. The levels of six proteins and their isoforms, including proapolipoprotein, apolipoprotein E, beta-2 microglobulin, retinol-binding protein, transthyretin, and ubiquitin, were significantly altered in CSF of AD patients. The most prominently altered proteins were the apolipoproteins, especially proapolipoprotein.
Davidsson
Pia
P
Department of Clinical Neuroscience, Experimental Neuroscience Section, Göteborg University, Sahlgrenska University Hospital/Mölndal, SE-431 80, Sweden.
Westman-Brinkmalm
Ann
A
Nilsson
Carol L
CL
Lindbjer
Maria
M
Paulson
Linda
L
Andreasen
Niels
N
Sjögren
Magnus
M
Blennow
Kaj
K
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016001:Confidence Intervals; D005260:Female; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D010361:Patients / Q000706:statistics & numerical data; D020033:Protein Isoforms / Q000134:cerebrospinal fluid; D011506:Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D018709:Statistics, Nonparametric
D020033:Protein Isoforms; D011506:Proteins; D020543:Proteome