[ET-1 induces the expression of prooncogene c-fos in cultured neonatal rat myocardial cells].
In the present study, the effect of endothelin-1 (ET-1) on the expression of proto-oncogene c-fos in cultured neonatal rat myocardial cells was investigated. The results are as follows: ET-1 induced c-fos expression in a dose-dependent manner. Selective ET(A) receptor antagonist blocked ET-1-induced responses. Protein kinase C(PKC) agonist PMA induced c-fos expression.PKC inhibitor staurosporine blocked ET-1 induced c-fos expression. Calcium channel blocker, nifedipine did not significantly affect the expression of c-fos induced by ET-1. These results suggest that in cultured neonatal cardiomyocytes, ET-1 induced c-fos gene expression is mediated by ET(A) receptor with the participation of protein kinase C, while the voltage-dependent L-type Ca(2+) channel is not involved.
Wu
B
B
Department of Physiology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089.
Wang
T H
TH
Zhu
X N
XN
Pan
J Y
JY
D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1 / Q000494:pharmacology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D065128:Endothelin Receptor Antagonists; D019332:Endothelin-1; D010456:Peptides, Cyclic; D016760:Proto-Oncogene Proteins c-fos; D011493:Protein Kinase C; C072247:cyclo(Trp-Asp-Pro-Val-Leu)