title: Ion trap mass spectrometry in the structural analysis of haemoglobin peptides modified by epichlorohydrin and diepoxybutane.
abstract: Ion trap mass spectrometry has been shown to be particularly suitable for the structural analysis of high molecular weight peptides directly fragmented in the mass analyser without needing further sub-digestion reactions. Here we report the advantages of using multi-stage ion trap mass spectrometry in the structural characterisation of haemoglobin alkylated with epichlorohydrin and diepoxybutane. Alkylated globins were digested with trypsin and the peptide mixtures were analysed by MS(3). This technique allows the sequential fragmentation of peptides under analysis, giving rise to MS(3) product ion spectra with additional information with respect to MS(2) mass spectra. The results obtained complete the previously reported structural characterisation of alkylated haemoglobin, demonstrating the potential of ion trap mass spectrometry.
authors.affiliation: Centro Internazionale di Servizi di Spettrometria di Massa e Rete di Spettrometria di Massa - Istituto di Scienze dell'Alimentazione del C.N.R., Via Roma 52, I-83100 Avellino, Italy. nadia.miraglia@unina2.it
authors.forename: Nadia
authors.initials: N
authors.lastname: Miraglia
authors.forename: Adriana
authors.initials: A
authors.lastname: Basile
authors.forename: Maria
authors.initials: M
authors.lastname: Pieri
authors.forename: Antonio
authors.initials: A
authors.lastname: Acampora
authors.forename: Livia
authors.initials: L
authors.lastname: Malorni
authors.forename: Beatrice
authors.initials: B
authors.lastname: De Giulio
authors.forename: Nicola
authors.initials: N
authors.lastname: Sannolo
mesh_terms: D004811:Epichlorohydrin / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010455:Peptides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004852:Epoxy Compounds; D006454:Hemoglobins; D010455:Peptides; D004811:Epichlorohydrin; C007366:diepoxybutane