Short communication: Hepatic gene expression for gluconeogenic enzymes in lactating dairy cows treated with bovine somatotropin.
Eight lactating Holstein dairy cows (80 d in milk) were used to examine the effects of exogenous bovine somatotropin (bST) on hepatic contents of mRNA encoding pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), and microsomal triglyceride transfer protein (MTP). Concentrations of bST in plasma were higher and milk production increased 20% in bST-treated cows. Liver samples from cows treated with bST had significantly higher total lipid contents than those from control cows. Although there were small numerical tendencies, neither triglyceride concentrations in liver nor nonesterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA), or glucose in plasma differed significantly between bST-treated and control cows. Short-term bST treatment had no detectable effects on contents of PC, PEPCK, and MTP mRNA in the liver. In summary, exogenous bST stimulation of milk production is not mediated through enhanced liver gluconeogenesis, but may involve partitioning of glucose and fatty acids for preferential use by the mammary gland.
Pershing
R A
RA
Department of Animal Sciences University of Florida, Gainesville 32611, USA.
Moore
S D
SD
Dinges
A C
AC
Thatcher
W W
WW
Badinga
L
L
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology*; D007774:Lactation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology*; D008892:Milk / Q000378:metabolism*; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000378:metabolism; D011766:Pyruvate Carboxylase / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis
D002352:Carrier Proteins; D005230:Fatty Acids, Nonesterified; D012333:RNA, Messenger; C106092:microsomal triglyceride transfer protein; D013006:Growth Hormone; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D011766:Pyruvate Carboxylase; D005947:Glucose