title: [Overexpression of EDAG-1 in NIH3T3 cells leads to malignant transformation].
abstract: To study the biological function of EDAG-1, a novel gene cloned by our lab recently, the EDAG-1 cDNA was subcloned into pcDNA3. 1(+) vector and transfected into NIH3T3 cells by Lipofect AMINE methods. Overexpression of EDAG-1 in NIH3T3 cells resulted in loss of cell contact inhibition, anchorage-independent growth in soft agar and tumor formation in nude mice. These results suggest that EDAG-1 is a transforming gene and may be associated with the development of tumors, especially in hematopoietic system.
authors.affiliation: Institute of Infectious Disease, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 710006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lü
authors.forename: Wang-Xiang
authors.initials: WX
authors.lastname: Xu
authors.forename: Si-Ying
authors.initials: SY
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Chang-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Cai
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004195:Disease Models, Animal; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*
chemical_list: D011506:Proteins