Bisphenol A in combination with insulin can accelerate the conversion of 3T3-L1 fibroblasts to adipocytes.
The confluent cultures of 3T3-L1 fibroblasts were treated with or without bisphenol A (BPA) for 2 days and subsequently treated with insulin (INS) alone for 9 days. When BPA was absent during the first 2-day treatment period, the cultures contained 1.6 microg/microg DNA of triacylglycerol (TG), 202 mU/mg DNA of lipoprotein lipase (LPL) activity, and 462 nmol/min/mg DNA of glycerol-3-phosphate dehydrogenase (GPDH) activity. The presence of BPA during the same period caused a 150% increase in the TG content, a 60% increase in the LPL activity, and a 500% increase in the GPDH activity. Thus, BPA by itself can trigger 3T3-L1 fibroblasts to differentiate into adipocytes. Next, the confluent cultures were treated with BPA for 2 days and subsequently treated with a combination of INS and BPA for 9 days. The simultaneous presence of BPA with INS caused a 370% increase in the TG content, a 200% increase in the LPL activity, and a 225% increase in the GPDH activity compared with the cultures treated with INS alone. The amount of [(3)H]thymidine incorporated into DNA was lower in the cultures treated with INS in the presence of BPA than in those treated with INS alone, indicating that BPA has an anti-proliferative activity on 3T3-L1 cells. Taken together, our results indicate that BPA in combination with INS can accelerate the adipocyte conversion.
Masuno
Hiroshi
H
Department of Medical Laboratory Technology, Ehime College of Health Science, Takooda, Tobe-cho, Iyo-gun, Ehime 791-2101, Japan. hmasuno@ehime-chs.ac.jp
Kidani
Teruki
T
Sekiya
Keizo
K
Sakayama
Kenshi
K
Shiosaka
Takahiko
T
Yamamoto
Haruyasu
H
Honda
Katsuhisa
K
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001559:Benzhydryl Compounds; D002454:Cell Differentiation / Q000187:drug effects*; D004247:DNA / Q000096:biosynthesis; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000378:metabolism; D051379:Mice; D010636:Phenols / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013936:Thymidine / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004247:DNA; D004968:Estrogens, Non-Steroidal; D005993:Glycerolphosphate Dehydrogenase; D007328:Insulin; D008071:Lipoprotein Lipase; D010636:Phenols; D018809:Proliferating Cell Nuclear Antigen; D013936:Thymidine; D014280:Triglycerides; C006780:bisphenol A; D000098826:Bisphenol A Compounds