Yersinia pseudotuberculosis produces a cytotoxic necrotizing factor.
Cell extracts from Yersinia pseudotuberculosis induced multinucleation in HEp-2 cells in a manner similar to the effect caused by Escherichia coli cytotoxic necrotizing factor (CNF). The activity was not dependent on the Yersinia 70-kb virulence plasmid, and the activity was not inhibited by antibodies capable of neutralizing E. coli CNF type 1. The nucleotide sequence of the Yersinia cnf gene was 65.1% identical to the E. coli cnf gene.
Lockman
Hank A
HA
Children's Research Institute, Children's Hospital, Columbus, Ohio 43205-2696, USA. lockmanh@pediatrics.ohio-state.edu
Gillespie
Rebecca A
RA
Baker
Beth D
BD
Shakhnovich
Elizabeth
E
D000595:Amino Acid Sequence; D001427:Bacterial Toxins / Q000032:analysis* / Q000235:genetics / Q000633:toxicity; D001483:Base Sequence; D003603:Cytotoxins / Q000032:analysis* / Q000235:genetics / Q000633:toxicity; D004269:DNA, Bacterial / Q000737:chemistry; D029968:Escherichia coli Proteins; D006801:Humans; D008969:Molecular Sequence Data; D015011:Yersinia pseudotuberculosis / Q000472:pathogenicity*
D001427:Bacterial Toxins; D003603:Cytotoxins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1