title: Tumor immunity within the central nervous system stimulated by recombinant Listeria monocytogenes vaccination.
abstract: Tumors arising within the central nervous system (CNS) present the immune system with a challenging target, given the heterogeneous nature of these neoplasms and their location within an "immunologically privileged" site. We used the lymphocytic choriomeningitis virus nucleoprotein (LCMV-NP) as a pseudotumor antigen to investigate recombinant Listeria monocytogenes as a tumor vaccine against s.c. and intracerebral challenges with a NP-expressing glioma, 9L-NP. Using Fischer 344 rats, we demonstrate that vaccination with recombinant L. monocytogenes-NP stimulates protection against s.c., but not intracerebral, 9L-NP tumor challenge in an antigen-specific, CD8(+) T-cell-dependent manner. After s.c. tumor rejection, enhanced antitumor immunity is achieved via epitope spreading that permits complete resistance against lethal intracerebral challenge with 9L-NP and with the untransfected parental 9L tumor. Unlike the CD8(+)-dependent immune responses against s.c. 9L-NP tumors, this expanded intracerebral immunity against endogenous tumor-associated antigens is dependent on both CD4(+) and CD8(+) T cells. Taken together, these results demonstrate that the mechanisms of tumor immunity within the brain are different from those elicited against non-CNS tumors. Furthermore, vaccination approaches exploiting the concept of epitope spreading may enhance the efficacy of antitumor immune responses within the immunologically privileged CNS, potentially mediating tumor cell killing through both CD4(+)- and CD8(+)-dependent effector pathways.
authors.affiliation: Division of Neurosurgery, Department of Microbiology, Immunology, & Molecular Genetics, School of Medicine, University of California at Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Liau
authors.forename: Eric R
authors.initials: ER
authors.lastname: Jensen
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Kremen
authors.forename: Sylvia K
authors.initials: SK
authors.lastname: Odesa
authors.forename: Steven N
authors.initials: SN
authors.lastname: Sykes
authors.forename: Michael C
authors.initials: MC
authors.lastname: Soung
authors.forename: Jeff F
authors.initials: JF
authors.lastname: Miller
authors.forename: Jeff M
authors.initials: JM
authors.lastname: Bronstein
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control / Q000628:therapy; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D018316:Gliosarcoma / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control / Q000628:therapy; D008089:Listeria monocytogenes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D019496:Cancer Vaccines; D000939:Epitopes; D009698:Nucleoproteins; D014614:Vaccines, Synthetic