Vero cell co-culture counteracts the detrimental effects of hydrosalpinx fluid on the development of mouse embryos in vitro.
Recent studies have suggested that the hydrosalpinx has a negative effect on pregnancy outcome, with markedly diminished implantation and increased early pregnancy loss. Fluid from the hydrosalpinx may leak into and accumulate in the uterine cavity. It is not clear, however if this creates a hostile local environment in the uterus for embryo implantation or exerts a direct embryotoxic effect. This study was conducted to investigate the detrimental effects of hydrosalpinx fluid (HSF) on the development of mouse embryos in vitro and to demonstrate whether Vero cells overcome these adverse effects. HSF was collected from three women with bilateral hydrosalpinx at the time of laparoscopic surgery. Collected fluid was centrifuged and the supernatant was frozen at -20 degrees C. For co-culture, Vero cells were commercially obtained in a frozen state and cultured using Ham's F10 medium. Single-cell mouse embryos (B6CBAF1) were cultured for 5 days in 0, 0.4, 0.8, and 1.2% of HSF in media with and without Vero cells and examined daily to record the number of embryos reaching expanded blastocyst and hatching stage. Co-culture of mouse embryos with Vero cells at 0.8% HSF concentration significantly enhanced embryo development, but not at 1.2% hydrosalpinx fluid concentration. These results suggest that HSF is highly embryotoxic and Vero cells are likely to overcome these detrimental effects to some degree.
Kim
Yong Bong
YB
Department of Obstetrics and Gynecology, College of Medicine, InJe University, Seoul Paik Hospital, Seoul, Korea. kimyb2@unitel.co.kr
Ahn
Sung Ho
SH
Chang
Doo Young
DY
Chung
Kyung Nam
KN
Koh
Jae Whoan
JW
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001826:Body Fluids / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D005314:Embryonic and Fetal Development; D005184:Fallopian Tube Diseases / Q000378:metabolism*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D014709:Vero Cells