Mice deficient in fractalkine are less susceptible to cerebral ischemia-reperfusion injury.
Fractalkine (FKN), also known as neurotactin, is a CX(3)C chemokine that exists in both secreted and neuronal membrane-bound forms and is upregulated during brain inflammation. There is accumulating evidence that FKN induces chemotaxis by binding to its receptor CX(3)CR1 on leukocytes and microglia. We generated FKN-deficient mice to study the role of FKN in postischemic brain injury. After transient focal cerebral ischemia, FKN-deficient mice had a 28% reduction in infarction size and lower mortality rate, when compared to wild-type littermates. The findings of this study indicate a possible role for FKN in augmenting postischemic injury and mortality after transient focal cerebral ischemia.
Soriano
Sulpicio G
SG
Department of Anesthesia, Children's Hospital, Boston, 300 Longwood Avenue, Boston, MA 02115, USA. sulpicio.soriano@tch.harvard.edu
Amaravadi
Lakshmi S
LS
Wang
Yanming F
YF
Zhou
Hong
H
Yu
Gary X
GX
Tonra
James R
JR
Fairchild-Huntress
Victoria
V
Fang
Qing
Q
Dunmore
Judy H
JH
Huszar
Dennis
D
Pan
Yang
Y
D000818:Animals; D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C / Q000172:deficiency / Q000235:genetics* / Q000276:immunology*; D004198:Disease Susceptibility / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D020244:Infarction, Middle Cerebral Artery / Q000276:immunology / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000276:immunology* / Q000473:pathology; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000032:analysis; D015427:Reperfusion Injury / Q000276:immunology* / Q000473:pathology
D054428:Chemokine CX3CL1; D020523:Chemokines, CX3C; C515539:Cx3cl1 protein, mouse; D008565:Membrane Proteins; D012333:RNA, Messenger