In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium.
OBJECTIVE
Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium.


METHODS
Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection.


RESULTS
LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2. LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32.


CONCLUSIONS
Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.
Hsieh
Yao-Yuan
YY
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
Lin
Chich-Sheng
CS
Sun
Yu-Ling
YL
Chang
Chi-Chen
CC
Tsai
Horng-Der
HD
Wu
Jackson Chieh-Hsi
JC
D000818:Animals; D018076:DNA, Complementary; D004717:Endometrium / Q000502:physiology*; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006131:Growth Inhibitors / Q000235:genetics*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR
D018076:DNA, Complementary; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008081:Liposomes; D008222:Lymphokines; D008156:Luciferases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases