title: Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers.
abstract: We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.
authors.affiliation: Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Meistermann
authors.forename: Virtudes
authors.initials: V
authors.lastname: Moreno
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Prieto
authors.forename: Erlend
authors.initials: E
authors.lastname: Moldrheim
authors.forename: Einar
authors.initials: E
authors.lastname: Sletten
authors.forename: Syma
authors.initials: S
authors.lastname: Khalid
authors.forename: P Mark
authors.initials: PM
authors.lastname: Rodger
authors.forename: Jemma C
authors.initials: JC
authors.lastname: Peberdy
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Isaac
authors.forename: Alison
authors.initials: A
authors.lastname: Rodger
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Hannon
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D008024:Ligands; D008670:Metals / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013053:Spectrophotometry; D013696:Temperature
chemical_list: D008024:Ligands; D008670:Metals; D004247:DNA; D007501:Iron