title: Simultaneous separation of 17 inorganic and organic arsenic compounds in marine biota by means of high-performance liquid chromatography/inductively coupled plasma mass spectrometry.
abstract: A method using high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS) has been developed to determine inorganic arsenic (arsenite, arsenate) along with organic arsenic compounds (monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, arsenocholine, trimethylarsine oxide, tetramethylarsonium ion and several arsenosugars) in fish, mussel, oyster and marine algae samples. The species were extracted by means of a methanol/water mixture and a dispersion unit in 2 min, with extraction efficiencies ranging from 83 to 107% in the different organisms. Up to 17 different species were determined within 15 min on an anion-exchange column, using a nitric acid gradient and an ion-pairing reagent. As all species are shown in one chromatogram, a clear overview of arsenic distribution patterns in different marine organisms is given. Arsenobetaine is the major compound in marine animals whereas arsenosugars and arsenate are dominant in marine algae. The method was validated with CRM DORM-2 (dogfish muscle). Concentrations were within the certified limits and low detection limits of 8 ng g(-1) (arsenite) to 50 ng g(-1) (arsenate) were obtained.
authors.affiliation: GALAB, Max-Planck-Str. 1, D-21502 Geesthacht, Germany. Ute.Kohlmeyer@galab.de
authors.forename: Ute
authors.initials: U
authors.lastname: Kohlmeyer
authors.forename: Jürgen
authors.initials: J
authors.lastname: Kuballa
authors.forename: Eckard
authors.initials: E
authors.lastname: Jantzen
mesh_terms: D000818:Animals; D001151:Arsenic / Q000032:analysis*; D001152:Arsenicals / Q000032:analysis*; D049872:Bivalvia / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D056890:Eukaryota / Q000737:chemistry*; D005399:Fishes / Q000378:metabolism*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008872:Microwaves; D010125:Ostreidae / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis; D012997:Solvents
chemical_list: D001152:Arsenicals; D007202:Indicators and Reagents; D012997:Solvents; D001151:Arsenic