Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1.
Aerobic biotransformation of the diaryl ethers 2,7-dichlorodibenzo-p-dioxin and 1,2,3,4-tetrachlorodibenzo-p-dioxin by the dibenzo-p-dioxin-utilizing strain Sphingomonas wittichii RW1, producing corresponding metabolites, was demonstrated for the first time. Our strain transformed 2,7-dichlorodibenzo-p-dioxin, yielding 4-chlorocatechol, and 1,2,3,4-tetrachlorodibenzo-p-dioxin, producing 3,4,5,6-tetrachlorocatechol and 2-methoxy-3,4,5,6-tetrachlorophenol; all of these compounds were unequivocally identified by mass spectrometry both before and after N,O-bis(trimethylsilyl)-trifluoroacetamide derivatization by comparison with authentic standards. Additional experiments showed that strain RW1 formed a second metabolite, 2-methoxy-3,4,5,6-tetrachlorophenol, from the original degradation product, 3,4,5,6-tetrachlorocatechol, by methylation of one of the two hydroxy substituents.
Hong
Hyo-Bong
HB
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
Chang
Yoon-Seok
YS
Nam
In-Hyun
IH
Fortnagel
Peter
P
Schmidt
Stefan
S
D001711:Biotransformation; D002396:Catechols / Q000032:analysis; D004147:Dioxins / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000378:metabolism*; D020578:Sphingomonas / Q000378:metabolism*
D002396:Catechols; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C423629:3,4,5,6-tetrachlorocatechol; C000925:2,7-dichlorodibenzo-4-dioxin; C092155:1,2,3,4-tetrachlorodibenzodioxin; C038861:4-chlorocatechol