title: Agaricus bisporus and Coprinus bilanatus TRP2 genes are tri-functional with conserved intron and domain organisations.
abstract: Cloned homobasidiomycete TRP2 genes for Agaricus bisporus and Coprinus bilanatus were sequence-characterised. Both genes encode tri-functional proteins with activity domains for glutamine amidotransferase (GAT; G domain), indole glycerol phosphate synthase (InGP; C domain) and phosphoribosyl anthranilate isomerase (F domain). A conserved intron disrupts the GAT-coding sequence in both genes. Consensus amino acid (aa) signatures were identified for GAT and InGP, but in the latter 15-aa signature, one residue did not fit the previously defined consensus. Protein architecture and parsimony analysis with analogous proteins indicate domain organisation (NH(2)-G-C-F-COOH) was as for other filamentous fungi. The data do not support earlier suggestions that the three activity domains are detached in A. bisporus.
authors.affiliation: Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, UK. mike.challen@hri.ac.uk
authors.forename: Michael P
authors.initials: MP
authors.lastname: Challen
authors.forename: Cunjin
authors.initials: C
authors.lastname: Zhang
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Elliott
mesh_terms: D000364:Agaricus / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D003302:Coprinus / Q000201:enzymology* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
chemical_list: D005656:Fungal Proteins