Construction of the novel conformationally-restricted peptide library for screening of peptides that control the interaction between nucleobases.
A unique conformationally-restricted peptide library was constructed using a loop structure as a structural scaffold. This library was used for the screening of the amino acid sequences that control the interaction between nucleobase triplets. The peptides have PNAs at the C-terminus as the recognition site and the random amino acid sequence at the N-terminus as the effector for the interaction between PNA and its complementary DNA triplets. From the peptide libraries constructed by the positional scanning method, the sequences that affect the interaction between PNA and complementary DNA were selected. The difference in the characteristic results by using A-T and G-C pairs was presented. This study would also give us some useful information about interaction between peptides and nucleic acids, such as relevances between these biomolecules in a prebiotic era.
Takahashi
Mizuki
M
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 226-8501, Yokohama, Japan.
Ueno
Akihiko
A
Mihara
Hisakazu
H
D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D006801:Humans; D019151:Peptide Library; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011487:Protein Conformation
D019151:Peptide Library; D020135:Peptide Nucleic Acids; D004247:DNA