[Study on expression of cell cycle-related genes in subclonal cell lines of human cervical carcinoma].
OBJECTIVE
To explore the role of expression of cell cycle-related genes in cervical carcinoma cell lines.


METHODS
A series of expression microarray analysis of two homologous cervical carcinoma subclonal cell lines were initiated by cDNA microarray which represent a set of 234 human cell cycle-related genes.


RESULTS
In normal medium, the percent of G(1) phase in CS03 cells was higher than in CS07 cells dramatically, but the percent of S phase in CS07 cells was more than in CS03 cells. After cultured 48 h in serum-free medium, the percent of apoptosis cells (sub-G(1) phase) in CS03 cells was higher than in CS07 cells significantly and increased with time. By applying this cDNA microarray, we identified 3 differentially expressed genes in two homologous cell lines, which were BN51, hsp90 and Mcl-1 genes, further identified 3 upregulated genes in CS07 cell line, the ratio of Cy5/Cy3 was 0.480, 0.479 and 0.490 respectively.


CONCLUSIONS
Differential expression of BN51, hsp90 and Mcl-1 genes is associated to various growth patterns of human cervical carcinoma cells.
Chen
Wei
W
Center of Experimental Medicin, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Li
Xu
X
Fu
Wei
W
Yang
Yucong
Y
Chen
Lihong
L
Cheng
Xiaoli
X
D017209:Apoptosis / Q000502:physiology; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D016895:Culture Media, Serum-Free; D005260:Female; D016193:G1 Phase / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D019253:Proto-Oncogene Proteins c-bcl-2; D012320:RNA Polymerase III / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D016196:S Phase / Q000502:physiology*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
D018797:Cell Cycle Proteins; D016895:Culture Media, Serum-Free; D018841:HSP90 Heat-Shock Proteins; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; C404765:POLR3D protein, human; D012320:RNA Polymerase III