title: Induction of nuclear matrix-estradiol receptor complex during capacitation process in human spermatozoa.
abstract: Estrogen receptor levels were determined in capacitated and noncapacitated sperm cells of normospermic men by the charcoal dextran method. The estrogen receptor of the nuclear matrix was released using hypertonic solutions. The distribution of the estrogen receptor was higher by 30% in capacitated spermatozoa as related to noncapacitated spermatozoa. The values of estrogen receptors in capacitated and noncapacitated spermatozoa were 91 +/- 21 fmol/10(8) sperm cells and 26 +/- 7 fmol/10(8) sperm cells, respectively. The association of estrogen receptor-nuclear matrix may be an important factor in the regulation of the transcription of the sperm genome of capacitated spermatozoa during the pronuclei stage.
authors.affiliation: Unidad de Investigación Médica en Biología Reproductiva, Subjefatura de Investigación Biomédica, Instituto Mexicano del Seguro Social, D.F., México. leo_calzada@yahoo.com
authors.forename: L
authors.initials: L
authors.lastname: Calzada
authors.forename: J M
authors.initials: JM
authors.lastname: Martinez
mesh_terms: D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D008297:Male; D015530:Nuclear Matrix / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism*; D013075:Sperm Capacitation / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol