[Transplantation of cryopreserved carotids in the rabbit: effect of cryoprotective agents].
The cryopreservation of blood vessels has been carried out for some decades with variable results. In order to study the behaviour of cryopreserved allografts by using new technique of cryopreservation a study on the ultrastructure arterial tissue consequences by microscopic techniques has been carried. The graft has been harvested from carotid artery of donor rabbit, implanted to a carotid artery of a recipient rabbit and extracted at 1, 3 and 5 months after the operation. An intimal thickening and a cellular loss in the media have been observed. However, good patency has been observed in all cryopreserved allografts. Manifestations of rejection are delayed in groups with polyethylene glycol.
M'Bengue-Gaye
A
A
Laboratoire de Résonance Magnétique Nucléaire et de Physiologie, Hôpital Saint-Louis, Paris.
Fleury
J P
JP
Gerota
J
J
Gueye
L
L
Eugene
M
M
Cisse
F
F
D000818:Animals; D002072:Butylene Glycols / Q000494:pharmacology*; D002339:Carotid Arteries / Q000187:drug effects / Q000637:transplantation* / Q000648:ultrastructure; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure; D006084:Graft Rejection; D006085:Graft Survival; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011817:Rabbits; D014021:Tissue Preservation; D017539:Tunica Intima / Q000187:drug effects / Q000648:ultrastructure; D017540:Tunica Media / Q000187:drug effects / Q000648:ultrastructure
D002072:Butylene Glycols; D003451:Cryoprotective Agents; D003470:Culture Media; D011092:Polyethylene Glycols; C026978:2,3-butylene glycol; D004121:Dimethyl Sulfoxide