title: Pressure volume curve and alveolar recruitment/de-recruitment. A morphometric model of the respiratory cycle.
abstract: The changes in pulmonary volume taking place during respiration are accompanied by the opening and closing of the alveoli, with the number of alveoli open, at the same transpulmonary pressure (TPP) differing, depending on whether the lung is insufflated or deflated.
  
  
  
  Seventy 344 Fischer rats divided into five groups. Group 1 lungs were fixed by instilling 10% formalin through the trachea to a pressure of 25 cm H2O. The lungs of the next four groups were air-filled and fixed via the pulmonary artery: group 2 lungs were fixed in inflation at 10 cm H2O TPP; group 3 lungs were fixed in inflation at 20 cm. H2O TPP; the lungs of groups 4 and 5 were fixed in deflation and, therefore, were inflated with air up to 27 cm. H2O to drop to 20 cm in group 4 and to 10 cm in group 5. The lungs were processed for light microscopy, carrying out a morphometric study. The results were statistically processed.
  
  
  
  The lungs insufflated with liquid fixative at 25 cm of TPP reached higher values in the variables Pulmonary Volume, Internal Alveolar Surface (IAS) and Number of Alveoli, being statistically significant (p < 0.05) in comparison with the other four groups. In the lungs fixed in deflation, the pulmonary volume, IAS and number of alveoli were greater than in those fixed in inflation. The lungs fixed to 20 cm in deflation displayed significant statistical differences compared with those fixed to 20 cm in inflation. The IAS and number of alveoli gave good rates in relation with the pulmonary volume (r > or = 0.65). Three variables were used to measure the size of the alveoli, alveolar cord, alveolar surface and Lm, but none showed significant modifications.
  
  
  
  This study supports the hypothesis that changes in lung volume are related to the increase/decrease in the number of alveoli that are open/closed and not to the modification in the size of the alveoli. Alveolar recruitment is the microscopic expression of pulmonary hysteresis, since the number of alveoli open in deflation is greater than the number open during inflation.
authors.affiliation: Morphological Science Department, Faculty of Medicine, University of Zaragoza, Spain. jescolar@posta.unizar.es
authors.forename: J D
authors.initials: JD
authors.lastname: Escolar
authors.forename: M A
authors.initials: MA
authors.lastname: Escolar
authors.forename: J
authors.initials: J
authors.lastname: Guzmán
authors.forename: M
authors.initials: M
authors.lastname: Roqués
mesh_terms: D000818:Animals; D008176:Lung Volume Measurements; D008953:Models, Anatomic; D023421:Models, Animal; D011650:Pulmonary Alveoli / Q000502:physiology*; D012123:Pulmonary Ventilation / Q000502:physiology*; D011669:Pulmonary Wedge Pressure / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344