title: [Measurement of calcium fluorescent intensity in vital brain slice and mechanism of brain injury after circulatory arrest].
abstract: OBJECTIVE
  To examine the calcium fluorescent intensity of vital brain slice and study the mechanism of brain injury after circulatory arrest.
  
  
  METHODS
  Eight pigs underwent 90 minutes' deep hypothermic circulation arrest (DHCA) and eight pigs underwent retrograde cerebral perfusion (RCP) through superior vena cava and then rewarmed for 120 minutes. Vital brain slices were obtained and the calcium fluorescent intensity was examined with laser confocal scanning microscope (LCSM). The morphological change was examined with light microscope and electron microscope.
  
  
  RESULTS
  The calcium fluorescent intensity of vital brain slice was lower in RCP group than in DHCA group. The calcium fluorescent intensity of vital brain slice is correlated with the level of moderate and severe eosinophilic degeneration of neuron (r = 0.86, P < 0.05).
  
  
  CONCLUSIONS
  Calcium overload contributes to the injury of neuron after DHCA. RCP remarkably attenuates calcium overload, thus protecting the brain tissue.
authors.affiliation: Beijing Anzhen Hospital, Beijing Capital Medical University, Beijing 100029, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Guan
authors.forename: Peiqing
authors.initials: P
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Caihong
authors.initials: C
authors.lastname: Wan
authors.forename: Meiling
authors.initials: M
authors.lastname: He
authors.forename: Shunping
authors.initials: S
authors.lastname: Du
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D004195:Disease Models, Animal; D005453:Fluorescence; D009410:Nerve Degeneration / Q000209:etiology*; D010477:Perfusion; D013552:Swine
chemical_list: D002118:Calcium