Use of a rapid immunochromatographic test for early diagnosis of dengue virus infection.
Acute serum samples collected from 92 patients were analysed for the presence of immunoglobulin M and G antibodies against dengue viruses using a rapid immunochromatographic (IC) test. Of 52 patients with dengue virus infection diagnosed using a standard hemagglutination inhibition test, 41 patients demonstrated dengue virus infection using the IC test. Half of the serum samples collected on day 3 after the onset of fever were positive for dengue antibodies, and all were positive on day 6 after symptom onset. In comparison with the hemagglutination inhibition test, the rapid IC test showed 79% sensitivity and 95% specificity. The efficiency of the test was 86%. The IC test showed good agreement with the hemagglutination inhibition test (k=0.72). The IC test is quick to perform and provides early diagnosis of dengue virus infection.
Kittigul
L
L
Department of Microbiology, Faculty of Public Health, Mahidol University, 420/1 Rajvithi Road, Bangkok 10400, Thailand. phlkt@mahidol.ac.th
Suankeow
K
K
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D002845:Chromatography / Q000379:methods*; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology* / Q000302:isolation & purification*; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006801:Humans; D008297:Male; D012680:Sensitivity and Specificity; D013997:Time Factors