title: Maize replicative alpha-type DNA polymerase: separation of polymerase and primase activities and recognition of primase subunits.
abstract: DNA polymerase and DNA primase activities in the maize alpha-type DNA polymerase 2 were dissociated and DNA polymerase-free DNA primase was studied. DNA primase synthesized primers that were 8-34 nucleotides long, with more intense bands at 15-17 nucleotides in length. DNA polymerase 1 (a putative delta-type enzyme) or DNA polymerase 2 were assayed after template-priming with purified DNA primase and showed a differential use of templates: whereas DNA polymerase 2 used a polydT template more efficiently than a natural template, DNA polymerase 1 used both of them poorly. The molecular size of DNA primase was estimated to be 68 kDa by gel filtration, western blotting and by a DNA primase 'trapping' assay.
authors.affiliation: aDepartamento de Bioquímica, Facultad de Química, UNAM, Ave. Universidad y Copilco, México DF, 04510, México bREGER, UMR 50 97 Université Bordeaux-CNRS, bat 3a-3eme étage, 146 rue Leo-Saignat, F-33076, Bordeaux Cedex, France.
authors.forename: Elpidio
authors.initials: E
authors.lastname: García
authors.forename: Patricia
authors.initials: P
authors.lastname: Laquel
authors.forename: Michel
authors.initials: M
authors.lastname: Castroviejo
authors.forename: Javier
authors.initials: J
authors.lastname: Plasencia
authors.forename: Jorge M
authors.initials: JM
authors.lastname: Vázquez-Ramos