Efficacy of common laboratory disinfectants on the infectivity of Cryptosporidium parvum oocysts in cell culture.
Nine liquid disinfectants were tested for their ability to reduce infectivity of Cryptosporidium parvum oocysts in cell culture. A 4-min exposure to 6% hydrogen peroxide and a 13-min exposure to ammonium hydroxide-amended windshield washer fluid reduced infectivity 1,000-fold. Other disinfectants tested (70% ethanol, 37% methanol, 6% sodium hypochlorite, 70% isopropanol, and three commercial disinfectants) did not reduce the infectivity after a 33-min exposure. The results indicate that hydrogen peroxide and windshield washer fluid or ammonium hydroxide disinfectant may be suitable laboratory disinfectants against C. parvum oocysts.
Weir
Susan C
SC
Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Pokorny
Nicholas J
NJ
Carreno
Ramon A
RA
Trevors
Jack T
JT
Lee
Hung
H
D064753:Ammonium Hydroxide; D000818:Animals; D018929:Cell Culture Techniques; D003049:Coccidiostats / Q000494:pharmacology; D016785:Cryptosporidium parvum / Q000187:drug effects*; D004202:Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006878:Hydroxides / Q000494:pharmacology; D007466:Iodophors / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology
D003049:Coccidiostats; D004202:Disinfectants; D006878:Hydroxides; D007466:Iodophors; D000431:Ethanol; D011092:Polyethylene Glycols; D064753:Ammonium Hydroxide; C015573:Wescodyne; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D000432:Methanol