Requirement of fibroblast growth factor 10 in development of white adipose tissue.
Fibroblast growth factors (FGFs) are important intercellular signaling molecules in developmental processes. Here, we show that FGF10 is secreted by cultured preadipocytes and that prevention of FGF10 signaling inhibits the expression of C/EBPbeta and the subsequent differentiation of these cells. An active form of C/EBPbeta rescued differentiation of the cells in which FGF10 signaling was blocked. Development of white adipose tissue and the expression of C/EBPbeta in this tissue of FGF10 knockout mice were markedly reduced, and the ability of embryonic fibroblasts derived from FGF10 knockout mice to differentiate into adipocytes was impaired. Therefore, FGF10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBPbeta through an autocrine/paracrine mechanism.
Sakaue
Hiroshi
H
Department of Clinical Molecular Medicine, Division of Diabetes, Digestive and Kidney Diseases, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
Konishi
Morichika
M
Ogawa
Wataru
W
Asaki
Toshiyuki
T
Mori
Toshiyuki
T
Yamasaki
Masahiro
M
Takata
Masafumi
M
Ueno
Hikaru
H
Kato
Shigeaki
S
Kasuga
Masato
M
Itoh
Nobuyuki
N
D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D000818:Animals; D019898:Autocrine Communication; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005938:Glucocorticoids / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D019899:Paracrine Communication; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology
D022782:CCAAT-Enhancer-Binding Protein-beta; C496464:Fgf10 protein, mouse; D051526:Fibroblast Growth Factor 10; D005938:Glucocorticoids; D007328:Insulin; D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors