Molecular cytogenetic analysis of the breakpoint region at 6q21-22 in T-cell lymphoma/leukemia cell lines.
Chromosome band 6q21 is reported to be one of the most frequent target regions in T-cell lymphoma for both translocations and deletions. To explore whether the breakpoint clustering in T-cell malignancy indicates the presence of a common breakpoint region in 6q, we employed fluorescence in situ hybridization analysis using various YAC, BAC, and PAC clones aligned at 6q21-22. We identified two T-cell lymphoma/leukemia cell lines with different differentiation stages that had breakpoints within the same novel gene, TCBA1 (T-cell lymphoma breakpoint associated target 1). In a T-cell lymphoblastic lymphoma cell line, HT-1, the TCBA1 fused to SUSP1 (SUMO-1-specific protease), creating a SUSP1-TCBA1 chimeric gene. However, in an adult T-cell leukemia cell line, ATN-1, no chimeric gene was detected, although aberrant TCBA1 transcripts were produced. We conclude that TCBA1 is a possible target gene for T-cell lineage-specific chromosome aberrations at 6q21.
Tagawa
Hiroyuki
H
Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan.
Miura
Ikuo
I
Suzuki
Ritsuro
R
Suzuki
Hiroko
H
Hosokawa
Yoshitaka
Y
Seto
Masao
M
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D019457:Chromosome Breakage / Q000235:genetics*; D022201:Chromosomes, Artificial / Q000235:genetics; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020451:Contig Mapping / Q000379:methods; D020732:Cytogenetic Analysis / Q000379:methods*; D004331:Drosophila melanogaster; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D015458:Leukemia, T-Cell / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000473:pathology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping / Q000379:methods; D010957:Plasmids / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; C460140:NKAIN2 protein, human