[Effects of vasoactive intestinal peptide on chemotaxis of bronchial epithelial cells].
To investigate the influence of vasoactive intestinal peptide (VIP) on chemotaxis of bronchial epithelial cells (BECs). Rabbit chemotactic migration of primary BEC was assessed in a blind-well Boyden chamber. Radioimmunoassay and radio-ligand affinity analysis were used for determining VIP secretion and vasoactive intestinal peptide receptor (VIPR) expression. The results showed: (1) the method for determining chemotaxis of BECs by using insulin as chemotactic factor was stable and reproducible (r=0.9703, P<0.01). (2) VIP (0.001-1 micromol/L) elicited chemotaxis of BECs which was substantial and concentration-dependent. The effects of VIP were inhibited by W-7 and H-7 (P<0.01). (3) Heat stress enhanced the secretion of VIP (P<0.01) and upregulated the expression of VIPR on BECs (P<0.05). These results indicate that VIP in the lungs may play an important role in the repair of damaged epithelium, accelerating restoration of the airway to its normal state. Calmodulin and protein kinase C may be involved in the signal transduction of VIP effects.
Guan
Cha-Xiang
CX
Department of Physiology, XiangYa Medical College, Central South University, Changsha 410078. guanchaxiang@hotmail.com
Zhang
Chang-Qing
CQ
Qin
Xiao-Qun
XQ
Luo
Zi-Qiang
ZQ
Zhou
Fu-Wen
FW
Sun
Xiu-Hong
XH
D000818:Animals; D001980:Bronchi / Q000166:cytology*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D018005:Receptors, Vasoactive Intestinal Peptide / Q000096:biosynthesis; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D007328:Insulin; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide