Kazal-type chymotrypsin inhibitor from duck pancreas.
A chymotrypsin inhibitor of the Kazal-type has been isolated from duck pancreas, by affinity chromatography on immobilized chymotrypsin, gel filtration on Bio-Gel P-10 and reverse phase (RP)-HPLC. It inhibits bovine chymotrypsin Aalpha with an association constant (K(a)) of 2.06x10(7) M(-1). The complete amino acid sequence was determined after digestion of pyridylethylated inhibitor with Staphylococcus aureus V8 protease and chemical cleavage with CNBr. Duck pancreatic chymotrypsin inhibitor (DPCI) was found to be a single polypeptide chain composed of 65 amino acid residues, corresponding to a molecular mass of 7191 Da.
Wilimowska-Pelc
Anna
A
Institute of Biochemistry and Molecular Biology, University of Wroclaw, Tamka 2, 50-137, Wroclaw, Poland. anna@bf.uni.wroc.pl
Olichwier
Zofia
Z
Mazurkiewicz
Aleksander
A
Kowalska
Jolanta
J
Wilusz
Tadeusz
T
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004372:Ducks; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D014359:Trypsin Inhibitor, Kazal Pancreatic; D002918:Chymotrypsin; C117608:alpha-chymotrypsin