Differential gene expression in the lens epithelial cells from selenite injected rats.
The mechanism causing loss of integrity of lens epithelial cells induced by an overdose of sodium selenite remains to be elucidated. The aim of the present experiment was to search for changes in gene expression in epithelial cells of lenses from rats developing selenite cataract. One day after injection of selenite into 12 day old rats, gene expression in lens epithelial cells was analysed using a commercial DNA array (Atlas Rat 1.2 Array). Changes were confirmed by RT-PCR. Of 1176 genes assayed by hybridization, 91 genes showed differences in expression between normal and selenite lenses. The three genes showing the greatest changes were: cytochrome c oxidase subunit I (COX-I, decrease), gastric inhibitory polypeptide (GIP, decrease), and early growth response protein-1 (EGR-1, increase). Both COX-I and EGR-1 have been reported to be involved with apoptosis. These results suggest that changes in COX-I and EGR-1 expression in lens epithelial cells might play important roles in apoptosis and altered metabolism leading to selenite cataract.
Nakajima
Takeshi
T
Senju Laboratory of Ocular Sciences, Oregon Health & Science University, West Campus, 505 N.W. 185th Avenue, Beaverton, OR 97006, U.S.A.
Nakajima
Emi
E
Fukiage
Chiho
C
Azuma
Mitsuyoshi
M
Shearer
Thomas R
TR
D000375:Aging; D000818:Animals; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism; D051545:Cyclooxygenase 1; D004268:DNA-Binding Proteins / Q000235:genetics; D051766:Early Growth Response Protein 1; D003576:Electron Transport Complex IV / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005749:Gastric Inhibitory Polypeptide / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D017874:Immediate-Early Proteins; D007527:Isoenzymes; D007908:Lens, Crystalline / Q000378:metabolism*; D008565:Membrane Proteins; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D011451:Prostaglandin-Endoperoxide Synthases; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018038:Sodium Selenite; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D005749:Gastric Inhibitory Polypeptide; D051545:Cyclooxygenase 1; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat; D003576:Electron Transport Complex IV; D018038:Sodium Selenite