[Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase].
The cultural conditions for the growth of Norcardia cell were studied in this paper. Controlling pH value, adding nutrient and optimizing the quantity of inducer during cultivation, the activity of nitrile hydratase reached 6567 u/mL (culture medium), which was the highest value appeared in native journals. In the farther hydratase experiments, no by-product, crylic acid, was detected. It showed that the activity of amidase was not promoted obviously while the activity of nitrile hydratase was increased greatly. The results set a strong foundation for the industrial application and the research on new technology.
Chen
Zhi
Z
Tsinghua University, Department of Chemical Engineering, Institute of Biochemical Engineering, Beijing 100094, China.
Sun
Xu-Dong
XD
Shi
Yue
Y
Shen
Zhong-Yao
ZY
Zhao
Jian-Xun
JX
Sun
Xiao-Ying
XY
D000178:Acrylamides / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009618:Nocardiaceae / Q000201:enzymology* / Q000378:metabolism
D000178:Acrylamides; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase; D005947:Glucose