title: DNA sequences coding for the F18 fimbriae and AIDA adhesin are localised on the same plasmid in Escherichia coli isolates from piglets.
abstract: The adhesin-involved-in-diffuse-adherence (AIDA) afimbrial adhesin is produced by human, but not by animal, Escherichia coli, with the exception of German porcine verotoxigenic Escherichia coli (VTEC) [Clin. Diagn. Lab. Immunol. 8 (2001) 143]. Presence and localisation of DNA sequences (aidA) coding for and production of an AIDA adhesin were investigated in a collection of Belgian VTEC and non-VTEC E. coli isolated from piglets at weaning time. The 174 isolates were also studied by colony hybridisation for the presence of DNA sequences coding for the Stx2e verocytotoxin and the F18 fimbrial adhesin (fed): 71 were Stx2+F18+AIDA+, 26 were F18+AIDA+, 12 were AIDA+, two were Stx2+AIDA+, and one was Stx2+ only. Fifty-four of the 58 (F18+)AIDA+ isolates tested positive in a western blotting assay with an immune serum raised against the AIDA protein. Hybridisation with the AIDA gene probe on plasmid DNA profiles identified a probe-positive plasmid band in the 10 AIDA+ and in 24 of the 25 F18+AIDA+ isolates studied. Moreover in F18+AIDA+ isolates, only one plasmid band hybridised with both F18 and AIDA probes. These results confirm the presence of aidA-related genes in not only VTEC, but also non-VTEC, isolates from piglets and the production of an antigenically AIDA-related protein by the majority of probe-positive E. coli. Moreover the plasmid DNA hybridisation results suggest a localisation on the same plasmid of the aidA- and fed-related DNA sequences.
authors.affiliation: Chaire de Bactériologie et de Pathologie des Maladies Bactériennes, Faculté de Médecine Vétérinaire, Université de Liège, Liège, Belgium. jg.mainil@ulg.ac.be
authors.forename: Jacques G
authors.initials: JG
authors.lastname: Mainil
authors.forename: Etienne
authors.initials: E
authors.lastname: Jacquemin
authors.forename: Pierre
authors.initials: P
authors.lastname: Pohl
authors.forename: Albert
authors.initials: A
authors.lastname: Kaeckenbeeck
authors.forename: Inga
authors.initials: I
authors.lastname: Benz
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D022603:Shiga Toxins / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014886:Weaning
chemical_list: D018830:Adhesins, Escherichia coli; D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; D022603:Shiga Toxins