title: Vitellogenin induction by 17beta-estradiol and 17alpha-ethinylestradiol in male zebrafish (Danio rerio).
abstract: Adult male zebrafish (Danio rerio) were exposed to 17beta-estradiol (E2) or 17alpha-ethinylestradiol (EE2) in flow-through systems for 8 days. This was done to compare the sensitivity of the estrogen inducible vitellogenin (Vtg) biomarker system of this proposed OECD test guideline species to other relevant test species. Vtg was quantified in whole body homogenate by a species-specific ELISA. Actual water concentrations of E2 and EE2 were quantified by LC-MS, with detection limits of 1.0 and 0.6 ng/l, respectively. Vtg induction (LOEC) occurred in whole body homogenate at actual water concentrations of 21 ng E2/l and 3.0 ng EE2/l, respectively. As an alternative to the ANOVA approach, the relationship between the percentage of responding fish (Vtg) and the external E2 or EE2 concentration was determined by logistic regression analysis. Based on the regression analysis, EC-values could be determined: EC10, EC50 and EC90 were 15.4, 41.2 and 67.1 ng E2/l, respectively and 0.92, 2.51 and 4.09 ng EE2/l, respectively. Comparisons of these response limits to corresponding values for rainbow trout (Oncorhynchus mykiss), fathead minnow (Pimephales promelas) and Japanese medaka (Oryzias latipes) revealed the zebrafish as a sensitive test species.
authors.affiliation: Institute of Biology, University of Southern Denmark, Odense, Campusvej 55, 5230, Odense M, Denmark.
authors.forename: Jon
authors.initials: J
authors.lastname: Rose
authors.forename: Henrik
authors.initials: H
authors.lastname: Holbech
authors.forename: Christian
authors.initials: C
authors.lastname: Lindholst
authors.forename: Ulrik
authors.initials: U
authors.lastname: Nørum
authors.forename: Allan
authors.initials: A
authors.lastname: Povlsen
authors.forename: Bodil
authors.initials: B
authors.lastname: Korsgaard
authors.forename: Poul
authors.initials: P
authors.lastname: Bjerregaard
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000032:analysis / Q000494:pharmacology*; D004997:Ethinyl Estradiol / Q000032:analysis / Q000494:pharmacology*; D016015:Logistic Models; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D014819:Vitellogenins / Q000096:biosynthesis*; D014867:Water / Q000032:analysis; D015027:Zebrafish
chemical_list: D014819:Vitellogenins; D014867:Water; D004997:Ethinyl Estradiol; D004958:Estradiol