Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients.
A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.
Boór
K
K
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
Rónai
Z
Z
Nemoda
Z
Z
Gaszner
P
P
Sasvári-Székely
M
M
Guttman
A
A
Kalász
H
H
D001483:Base Sequence; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D036103:Nanotechnology; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050638:Receptors, Dopamine D4; D019966:Substance-Related Disorders / Q000235:genetics*
D017931:DNA Primers; C493599:DRD4 protein, human; D017448:Receptors, Dopamine D2; D050638:Receptors, Dopamine D4