Effect of magnetic field exposure on calcium channel currents using patch clamp technique.
Calcium influxes through the membrane of PC-12D cells were measured under exposure to DC biased AC magnetic fields in resonant conditions of the ion cyclotron and the ion parametric resonance hypotheses and compared with influxes in cells without exposure to the magnetic field. After cancellation of the geomagnetic field, the cells were exposed to the horizontal fields generated by a current sheet, a planar sheet of conductor which generated a satisfactorily homogeneous horizontal magnetic field on the stage of a microscope without hindering treatment of a cell under observation. At or near any resonant conditions, no change in calcium influx could be detected under standard patch clamp conditions.
Obo
Mayumi
M
Mitsubishi Chemical Safety Institute, Ibaraki, Japan. m-Obo@ankaken.co.jp
Konishi
Shiro
S
Otaka
Yoshihisa
Y
Kitamura
Satoshi
S
D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D004574:Electromagnetic Fields; D007700:Kinetics; D016716:PC12 Cells; D018408:Patch-Clamp Techniques; D010673:Pheochromocytoma; D051381:Rats; D019789:Tetraethylammonium / Q000494:pharmacology
D015220:Calcium Channels; D002104:Cadmium; D019789:Tetraethylammonium; D002118:Calcium