An O-glycoside of sialic acid derivative that inhibits both hemagglutinin and sialidase activities of influenza viruses.
The compound Neu5Ac3alphaF-DSPE (4), in which the C-3 position was modified with an axial fluorine atom, inhibited the catalytic hydrolysis of influenza virus sialidase and the binding activity of hemagglutinin. The inhibitory activities to sialidases were independent of virus isolates examined. With the positive results obtained for inhibition of hemagglutination and hemolysis induced by A/Aichi/2/68 virus, the inhibitory effect of Neu5Ac3alphaF-DSPE (4) against MDCK cells was examined, and it was found that 4 inhibits the viral infection with IC50 value of 5.6 microM based on the cytopathic effects. The experimental results indicate that compound 4 not only inhibits the attachment of virus to the cell surface receptor but also disturbs the release of the progeny viruses from infected cells by inhibiting both hemagglutinin and sialidase of the influenza viruses. The study suggested that the compound is a new class of bifunctional drug candidates for the future chemotherapy of influenza.
Guo
Chao-Tan
CT
Department of Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi 422-8526, Japan.
Sun
Xue-Long
XL
Kanie
Osamu
O
Shortridge
Kennedy Francis
KF
Suzuki
Takashi
T
Miyamoto
Daisei
D
Hidari
Kazuya I-P Jwa
KI
Wong
Chi-Huey
CH
Suzuki
Yasuo
Y
D001665:Binding Sites; D002236:Carbohydrate Conformation; D004912:Erythrocytes / Q000276:immunology; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000201:enzymology*; D007700:Kinetics; D008081:Liposomes; D008958:Models, Molecular; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D012794:Sialic Acids / Q000737:chemistry / Q000494:pharmacology*
D006027:Glycosides; D008081:Liposomes; D012794:Sialic Acids; D009439:Neuraminidase