Chalcones from Chinese liquorice inhibit proliferation of T cells and production of cytokines.
Licochalcone A (LicA), an oxygenated chalcone, has been shown to inhibit the growth of both parasites and bacteria. In this study, we investigated the effect of LicA and four synthetic analogues on the activity of human peripheral blood mononuclear cell proliferation and cytokine production. Four out of five chalcones tested inhibited the proliferation of lymphocytes measured by thymidine incorporation and by flow cytometry. The production of pro- and anti-inflammatory cytokines from monocytes and T cells was also inhibited by four of five chalcones. Furthermore, intracellular detection of cytokines revealed that the chalcones inhibited the production rather than the release of the cytokines. Taken together, these results indicate that LicA and some analogues may have immunomodulatory effects, and may thus be candidates not only as anti-microbial agents, but also for the treatment of other types of diseases.
Barfod
Lea
L
Department of Clinical Microbiology, Centre for Medical Parasitology, Copenhagen University Hospital (Rigshospitalet), Denmark.
Kemp
Kåre
K
Hansen
Majbritt
M
Kharazmi
Arsalan
A
D000977:Antiparasitic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002599:Chalcone / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology*; D047188:Chalcones; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008516:Medicine, Chinese Traditional; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008517:Phytotherapy; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000977:Antiparasitic Agents; D047188:Chalcones; D016207:Cytokines; D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D002599:Chalcone; C070840:licochalcone A; D013755:Tetradecanoylphorbol Acetate