[Detection and quantification of HIV-1 provirus provides a prognostic marker for the therapy-monitoring].
By the effective application of highly active antiretroviral therapy (HAART), the plasma HIV-1 RNA level can be dramatically suppressed to below the detectable level in a short period. As the amount of total HIV-1 DNA in peripheral CD4+ cells sharply decline in the first phase of the treatment in accordance with the decline of HIV-1 RNA, total HIV-1 DNA, mainly containing the unintegrated form, can be a useful marker for therapy-monitoring just as HIV-1 RNA. In the next phase, after the transcription and reverse transcription of HIV-1 are profoundly inhibited by continuing HAART, the mass of the latent HIV-1 reservoir can be quantified by measuring the amount of total HIV-1 DNA which mainly reflects the integrated HIV-1 provirus in this phase.
Kaneda
Tsuguhiro
T
Department of Clinical Research, Nagoya National Hospital.
Wada
Kaoru
K
Hagiwara
Tomoko
T
Nagai
Hiromi
H
Shirasaka
Takuma
T
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000188:drug therapy*; D023241:Antiretroviral Therapy, Highly Active; D015415:Biomarkers / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D016903:Drug Monitoring; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D011379:Prognosis; D011533:Proviruses / Q000235:genetics*; D016662:Virus Integration
D015415:Biomarkers; D004279:DNA, Viral