Mucopolysaccharides from psyllium involved in wound healing.
Mucopolysaccharides derived from the husk of psyllium (Plantago ovata) have properties beneficial for wound cleansing and wound healing. Recent studies indicate that these mucopolysaccharides also limit scar formation. Our in vitro and in vivo studies aimed to investigate the mechanisms involved, e.g., fluid absorption, bacterial adherence and in vitro stimulatory effects on macrophages, which are pivotal in wound healing. The mucopolysaccharides contained in a sachet (Askina Cavity) or in a hydrocolloid mixture (Askina Hydro) were found to have a gradual and sustained absorbency over a period of 7 days, amounting to 4-6 times their weight in water. The swelling index was 9 mm after 312 h. Adherence of wound bacteria to the mucopolysaccharides started after 2 h and was more pronounced after 3 h. Semiquantitative measurements of bacterial adherence used centrifugation and subsequent optical density determinations of supernatant. These confirmed the strong adherence potential of psyllium particles. Lactic acid dehydrogenase staining of pretreated cultured human skin explants did not reveal toxicity of the mucopolysaccharides derived from psyllium husk. Langerhans' cell migration from the epidermis was negligible and interleukin-1 beta expression in the explants was not significant, supporting the very low allergenic potential of psyllium. The characteristics of mucopolysaccharide granulate derived from psyllium husk in Askina Cavity and Askina Hydro related to fluid absorption, bacterial adherence, biocompatibility, stimulation of macrophages, irritancy response and allergenicity showed an optimal profile, supporting the good clinical performance of wound healing products containing psyllium husk.
Westerhof
W
W
Department of Dermatology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1005AZ Amsterdam, The Netherlands. w.westerhof@amc.uva.nl
Das
P K
PK
Middelkoop
E
E
Verschoor
J
J
Storey
L
L
Regnier
C
C
D000042:Absorption; D000268:Adhesiveness; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects; D001458:Bandages; D003102:Colloids; D016207:Cytokines / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006168:Guinea Pigs; D006241:Haptens / Q000494:pharmacology; D006967:Hypersensitivity / Q000473:pathology; D007801:Langerhans Cells / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008422:Materials Testing; D009924:Organ Culture Techniques; D011620:Psyllium / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D012867:Skin / Q000473:pathology; D013552:Swine; D014945:Wound Healing / Q000187:drug effects*
D003102:Colloids; D016207:Cytokines; D006025:Glycosaminoglycans; D006241:Haptens; D011620:Psyllium