title: [The effect of high concentration of sodium and potassium ion on motility of human sperm preserved in electrolyte-free solution].
abstract: OBJECTIVE
  To assess the effect of high concentration of sodium and potassium ion on the motilities of human sperm following the preservation in electrolyte-free solution.
  
  
  METHODS
  Semen samples were obtained from patients attending our infertility clinic. The motilities of their sperm were more than 40%.
  
  
  METHODS
  Ejaculated sperm were washed by using the electrolyte-free Percoll gradient and then preserved in electrolyte-free solution at 4 degrees C for a week. The preserved sperm was incubated and analyzed after the addition of electrolyte solutions under acid or alkaline condition. The sperm were continuously preserved for a day at room temperature and analyzed.
  
  
  RESULTS
  The sperm was by the addition of 35-135 mM Na+ or 5-135 mM K+ in acid solutions. Under the alkaline conditions, sperm were reactivated without Na+ or K+. However, the addition of 135 mM Na+ or K+ significantly decreased in sperm motilities. The some sperm in the solution containing low concentration of Na+ or K+ ions maintained their motilities for a day at room temperature. However, all sperm lost their motilities in the 135 mM Na+ or K+ solutions.
  
  
  CONCLUSIONS
  The high concentration of Na+ or K+ ions had detrimental effects on motilities of sperm following the preservation in electrolyte-free solution.
authors.affiliation: Department of Urology, Yokohama City University School of Medicine.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yumura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Saito
authors.forename: Kotaro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Takehiko
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kanno
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sato
authors.forename: Akira
authors.initials: A
authors.lastname: Iwasaki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hosaka
mesh_terms: D006801:Humans; D008297:Male; D019188:Organ Preservation Solutions; D011188:Potassium / Q000494:pharmacology*; D012662:Semen Preservation / Q000379:methods; D012964:Sodium / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects*
chemical_list: D019188:Organ Preservation Solutions; D012964:Sodium; D011188:Potassium