title: Specific immune response to Parietaria judaica plant profilin: a low T cell proliferative response supports high IgE and skin prick test.
abstract: BACKGROUND
  allergic disease caused by Parietaria judaica (Pj) has been widely documented in Mediterranean area. Profilins have been identified as widely distributed allergenic proteins. The role of Pj profilin in specific immune response in Pj-sensitized patients is unknown.
  
  
  METHODS
  skin prick test and determination of specific and total IgE levels in serum were performed in all patients (n = 28) and non-allergic controls (n = 18). Peripheral blood mononuclear cells (PBMC) were isolated from both groups and stimulated with crude extract or highly purified Pj profilin. The production of type I and type II cytokines was determined by specific and polyclonal stimuli in patients and controls. T-cell lines specific to Pj profilin were established and cross-reactivity with another highly purified profilin from Phleum pratense (Phl p) was evaluated.
  
  
  RESULTS
  Pj profilin-sensitized patients showed a small but significantly increased in T-cell proliferative response to this profilin compared with non-atopic controls. The production of interleukin (IL)-4 and interferon (IFN)-&#947; in response to the specific stimulus was undetectable. However, the production of IL-4 in response to a polyclonal stimulus [phytohemagglutinin (PHA)] was significantly higher in atopic patients than in controls. The T-cell response did not correlate with the magnitude of response to skin prick tests with Pj profilin or with Pj-specific serum IgE levels. In addition, the production of IL-4 in response to a polyclonal stimulus (PHA) did not correlate with the individual skin prick tests to Pj profilin or with Pj-specific IgE levels in serum. The T-cell lines tested showed no cross-reactivity with Phl p profilin.
  
  
  CONCLUSIONS
  our results suggest that Pj profilin is partly responsible for the T-cell-mediated response in patients allergic to Pj. The high skin reactivity to Pj profilin is these patients was accompanied by a small increase in the T-cell response to this profilin. The response was highly specific since Pj profilin specific T-cell lines showed no cross-reactivity with a highly homologous profilin from Phl p. The lack of correlation between the proliferative T-cell response and polyclonal IL-4 production with allergen-specific serum IgE and skin reactivity probably indicates that some of the responding T-cells may be involved in immune reactions other than those supporting IgE production.
authors.affiliation: Service of Immundogy. IDIBAPS. Hospital Clínic, Barcelona. Spain.
authors.forename: D
authors.initials: D
authors.lastname: Benítez
authors.forename: P
authors.initials: P
authors.lastname: García-Ortega
authors.forename: C
authors.initials: C
authors.lastname: Picado
authors.forename: J
authors.initials: J
authors.lastname: Milà
authors.forename: J
authors.initials: J
authors.lastname: Vives
authors.forename: J
authors.initials: J
authors.lastname: Martínez
authors.forename: R
authors.initials: R
authors.lastname: Vilella
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D032403:Parietaria / Q000276:immunology; D010936:Plant Extracts / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology; D051304:Profilins / Q000276:immunology*; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D010936:Plant Extracts; D010940:Plant Proteins; D051304:Profilins; D007073:Immunoglobulin E; C014155:Parietaria judaica pollen