[Modification of repair DNA synthesis in mutagen-treated human fibroblasts during adaptive response and the antimutagenic effect of garlic extract].
Repair DNA synthesis (RDS) in human fibroblasts during the adaptive responses (ARs) induced by cadmium chloride (CdCl2), gamma-radiation, and 4-nitroquinoline-1-oxide (4NQO) was compared in cells pretreated and not pretreated with garlic extract. The RDS was increased during the ARs induced CdCl2 and gamma-irradiation. Garlic extract stimulated RDS in cells treated by the same mutagens. 3-Aminobenzamide (3AB), an inhibitor of poly(ADP-ribose) polymerase, decreased the RDS rate in cells treated with CdCl2 and gamma-irradiation but had no significant effect on cells treated with 4NQO. It was demonstrated that DNA repair was involved into cell protection in different ways in the cases of antimutagen treatment and AR.
L'vova
G N
GN
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia. lvova@vigg.ru
Zasukhina
G D
GD
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000222:Adaptation, Physiological / Q000187:drug effects; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001549:Benzamides / Q000494:pharmacology; D019256:Cadmium Chloride / Q000633:toxicity; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D005737:Garlic; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors
D016587:Antimutagenic Agents; D001549:Benzamides; D004791:Enzyme Inhibitors; D009153:Mutagens; D010936:Plant Extracts; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D015112:4-Nitroquinoline-1-oxide; C025160:3-aminobenzamide; D004247:DNA; D019256:Cadmium Chloride