Single-step transformation for generating marker-free transgenic rice using the ipt-type MAT vector system.
The ipt-type MAT vector uses the ipt gene for regeneration of marker-free transgenic plants. However, it was pointed out that this system was not suitable for most economically important crops that regenerated through auxin-dependent embryogenesis. We report a single-step transformation system of rice using MAT vector. When we transformed scutellum tissues of 5 days pre-cultured rice seeds, marker-free transgenic rice plants directly regenerated from 25.5% infected scutellum tissues without forming ipt-intermediates within 4 weeks after an infection. Excision of the ipt gene caused the regeneration of marker-free transgenic rice plants through embryogenic tissues. Therefore, this system needs no selective agent and no sexual crossing for identification of transgenic plants not containing a selectable marker gene. This system is highly effective for generation of marker-free transgenic plants in economically important crops.
Endo
Saori
S
Wood-Bioengineering, Pulp and Paper Research Laboratory, Nippon Paper Industries CO., LTD. 5-21-1, Oji, Kita-ku, Tokyo 114-0002, Japan.
Sugita
Koichi
K
Sakai
Miho
M
Tanaka
Hiroshi
H
Ebinuma
Hiroyasu
H
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
D018744:DNA, Plant; D005819:Genetic Markers; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase