title: Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.
abstract: The proteins under the control of the two-component system VirR/VirS in Clostridium perfringens were analyzed by using two-dimensional gel electrophoresis of the culture supernatant from the wild type and the virR mutant. Based on matrix-assisted laser desorption ionization-time of flight/mass spectrometry, seven positively regulated proteins and eight negatively regulated proteins were identified. Transcriptome analysis confirmed that 7 of the 15 proteins were regulated by the VirR/VirS system at the transcriptional level, but the remaining proteins were modified with a VirR/VirS-directed protease at the posttranslation and secretion levels. We purified and characterized the VirR/VirS-directed protease from the culture supernatant and identified it as a kind of clostripain. Because this proteolytic activity was strongly inhibited by leupeptin and antipain, it was concluded that this protease was a member of the family of cysteine proteases of C. perfringens.
authors.affiliation: Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Ten-nohdai, Tsukuba 305-8575, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shima
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Yoshino
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Yonezawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hideo
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification*; D003016:Clostridium perfringens / Q000737:chemistry / Q000235:genetics / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D020543:Proteome; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; C068267:mry protein, Streptococcus pyogenes; D012697:Serine Endopeptidases