title: Comparison of gas chromatography and liquid chromatography mass spectrometric measurements for high accuracy analysis of cholesterol in human serum by isotope dilution mass spectrometry.
abstract: Cholesterol measurements are of vital clinical importance and reliable reference materials are essential for method validation. Gas chromatography with mass spectrometry (GC/MS) is usually used for the high accuracy analysis of cholesterol by isotope dilution. A certified reference material for cholesterol content in human serum was analysed by isotope dilution utilising GC/MS and liquid chromatography mass spectrometry (LC/MS). The use of LC/MS avoided the need for a derivatisation step. Both LC/MS and GC/MS produced results on the measurement of cholesterol that agreed within 0.5% of the certified value. Moreover, the precision obtained for ratio measurement using both techniques are comparable and lead to relative expanded standard uncertainties (with a coverage factor of 2) varying between 0.2 and 0.5%.
authors.affiliation: LGC, Queens Road, Teddington, Middlesex TW11 0LY, UK. CWB@LGC.CO.UK
authors.forename: Céline S J Wolfe
authors.initials: CS
authors.lastname: Briche
authors.forename: David
authors.initials: D
authors.lastname: Carter
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Webb
mesh_terms: D002784:Cholesterol / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D011865:Radioisotope Dilution Technique; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D002784:Cholesterol