Impairment of male rat reproductive function in F1 offspring from dams exposed to 2-bromopropane during gestation and lactation.
The toxic effects of 2-bromopropane (2-BP) on the reproductive tracts of male F1 offspring from dams exposed to 2-BP during gestation and lactation were investigated. Ten pregnant (sperm-positive) Sprague-Dawley rats per group were exposed sc to 2-BP at 135, 405, and 1215 mg/kg/day from gestation day (GD) 6 to postnatal day (PND) 20. 2-BP decreased the proportion of dams littering at the two highest doses. At the highest dose, the rate of delivery and surviving pups were significantly lower than in the controls (P < or = 0.05). The relative weights of testes vs. brain were significantly lower than the controls (P < or = 0.05) on PND 33 and 63 at 405 mg/kg/day, and on PND 90 at 1215 mg/kg/day in the F1 rats. Seminiferous tubule atrophy, germ cell loss, and increased Leydig cell proliferation were observed at the highest dose by histopathologic examination. Female offspring has a decrease in all follicle types at the high dose. These results suggest that gestational and lactational exposure to 2-BP at a high maternally toxic dose impairs the development of the reproductive organs of the offspring.
Kang
Kyung-Sun
KS
Department of Veterinary Public Health, College of Veterinary Medicine, School of Agricultural Biotechnology, Seoul National University, 103 Seodun-dong, Kwonsun-gu, Suwon, 441-744, Korea.
Li
Guang-Xun
GX
Che
Jeong-Hwan
JH
Lee
Yong-Soon
YS
D000818:Animals; D001724:Birth Weight / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002452:Cell Count; D004822:Epididymis / Q000473:pathology; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005836:Genitalia, Female / Q000187:drug effects / Q000254:growth & development; D005837:Genitalia, Male / Q000187:drug effects / Q000254:growth & development; D006842:Hydrocarbons, Brominated / Q000008:administration & dosage / Q000633:toxicity*; D007774:Lactation / Q000502:physiology*; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000473:pathology
D006842:Hydrocarbons, Brominated; D009153:Mutagens; C103736:2-bromopropane