A potential membrane protein involved in pre-tRNA splicing of Schizosaccharomyces pombe.
We had previously isolated six pre-tRNA splicing mutants of Schizosaccharomyces pombe named ptp1 to ptp6. To investigate the molecular mechanism of tRNA splicing, we cloned the ptp4(+) gene by complementation of the temperature-sensitive growth defect. The ptp4(+) gene consists of three exons and encodes a putative protein of 218 amino acids with a molecular mass of 24.4 kDa. Analysis of the amino acid sequence reveals that the protein is a potential membrane protein with four membrane-spanning regions. The ptp4(+) shows significant similarity to the Saccharomyces cerevisiae putative protein YOR311C. Expression of the ptp4(+) gene in the ptp4(-) mutant restores the ability to splice tRNA. Northern blot analysis showed that the ptp4(+) gene is expressed in both mating-type cells of S. pombe. These results suggest that the Ptp4(+) could be a component involved in tRNA splicing.
Kim
Minjung
M
Department of Genetic Engineering, Chongju University, Chongju, South Korea.
Hwang
Kuchan
K
Lim
Chang Jin
CJ
Kim
Daemyung
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development
D008565:Membrane Proteins; D012322:RNA Precursors