A specific role for tocopherol and of chemical singlet oxygen quenchers in the maintenance of photosystem II structure and function in Chlamydomonas reinhardtii.
alpha-Tocopherol concentrations were determined at low and high light intensities and compared with the rate of photosynthesis, photosystem II (PS II) and its reaction center D1 protein. Blocking of tocopherol biosynthesis at the 4-hydroxyphenylpyruvate dioxygenase by the herbicide pyrazolynate led to a quick disappearance of alpha-tocopherol in high light, as well as of PS II activity and the D1 protein. Homogentisic acid rescued all activities. It is concluded that alpha-tocopherol has a continuous turnover as a scavenger of the singlet oxygen that arises from the quenching by oxygen of the triplet of the PS II reaction center and triggers the degradation of the D1 protein. Thus tocopherols are essential to keep photosynthesis active. We suggest that this is why plants make and need tocopherols. Chemical quenchers of singlet oxygen, notably diphenylamines, completely protect PS II, prevent D1 protein degradation and keep tocopherol levels even at very high light intensities. This supports the notion that 1O2 is the intermediate in light triggered D1 protein turnover.
Trebst
Achim
A
Plant Biochemistry, Ruhr-University Bochum, D-44780, Bochum, Germany. achim.trebst@ruhr-uni-bochum.de
Depka
Brigitte
B
Holländer-Czytko
Heike
H
D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000037:antagonists & inhibitors; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D006713:Homogentisic Acid / Q000494:pharmacology; D008027:Light; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism* / Q000528:radiation effects; D045332:Photosystem II Protein Complex; D026082:Singlet Oxygen / Q000378:metabolism*; D024505:Tocopherols / Q000378:metabolism*
D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D006540:Herbicides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D026082:Singlet Oxygen; D015111:4-Hydroxyphenylpyruvate Dioxygenase; D006713:Homogentisic Acid; D024505:Tocopherols