Free radicals-induced morphological changes in the pancreas.
Oxidative stress occurs whenever there is an imbalance between the generation of free radicals and the antioxidant defense. Oxygen is cytotoxic if oxygen concentration is higher than its amount in the atmosphere. This phenomenon is called hyperoxide process. This experiment was performed to determine the influence of normobaric hyperoxide process on peroxidation occurred in the rat's pancreas. Furthermore, the aim of this study was to estimate morphological changes in the pancreas. The studies were carried out on 44 male Wistar strain rats with the initial body weight of 230-250 g. After acclimation period the animals were divided into four experimental groups of 11 in a group. The exposure period of the influence of chemically pure oxygen was the base of the classification of rats into experimental groups labelled as follows: I, II, III. The time of exposure was 12, 24 and 48 hrs, respectively. The animals were dissected immediately after decapitation. The internal organs were investigated during autopsy. The whole pancreas with surrounding fatty tissue and peritoneum was cut out. Ultrastructural identification of different cell types required examination of several sections from each block and careful examination of numerous selected photographs. The histological preparations were evaluated in electron microscope. Histological evaluation revealed the presence of changes which seem to be adaptative and temporary. The B cells of Langerhans' islets were more damaged than other endocrine cells.
Janicki
K
K
1st Department of Surgery and Transplantology, Medical University of Lublin.
Radzikowska
E
E
Pietura
R
R
Siezieniewska
Z
Z
D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D005609:Free Radicals / Q000633:toxicity; D008297:Male; D018384:Oxidative Stress; D010100:Oxygen / Q000008:administration & dosage; D010179:Pancreas / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000633:toxicity*; D022142:Secretory Vesicles / Q000648:ultrastructure
D005609:Free Radicals; D017382:Reactive Oxygen Species; D010100:Oxygen