Studies on the biosynthesis of phomoidride B (CP-263,114): evidence for a decarboxylative homodimerization pathway.
[reaction: see text]. Feeding experiments using a deuterium labeled C16 maleic anhydride and whole cell culture of ATCC 74256 led to the isolation of phomoidride B with deuterium incorporation at C(7) and C(19) as determined by 2H NMR and electrospray mass spectrometry. This result is in accord with a decarboxylative homodimerization of the C16 maleic anhydride as a key biosynthetic step leading to phomoidride B.
Sulikowski
Gary A
GA
Department of Chemistry, Texas A&M University, College Station, Texas 77842, USA. sulikowski@mail.chem.tamu.edu
Agnelli
Fabio
F
Spencer
Paul
P
Koomen
John M
JM
Russell
David H
DH
D003653:Decarboxylation; D003903:Deuterium; D009682:Magnetic Resonance Spectroscopy; D008299:Maleic Anhydrides / Q000737:chemistry*; D010407:Penicillium / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
C104957:CP 263114; D008299:Maleic Anhydrides; D003903:Deuterium