Comparison of the selenium level with GSH-Px activity in the liver of mice treated with 7,12 DMBA.
In this study, the relationship between selenium (Se) and glutathione peroxidase (GSHPx) levels was investigated in 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-treated mouse liver. The potential mammary carcinogen 7,12-DMBA, was injected intraperitoneally (20 mg kg(-1) day(-1)) into 10-12 month old female mice. After 21 days of application the mice were sacrificed and GSHPx and Se levels of liver homogenates were measured. Se and GSHPx levels in 7,12-DMBA-treated mice were significantly lower than those of controls (p < 0.05). The control group exhibited 0.9 +/- 0.066 U mg(-1) protein and 0.86 +/- 0.058 p.p.m. levels of GSHPx and Se respectively. The 7,12-DMBA-treated group had significantly (p < 0.05) decreased GSHPx and Se levels (0.42 +/- 0.062 U mg(-1) protein and 0.69 +/- 0.034 p.p.m. respectively). The results show a direct relationship between Se and GSHPx activity and a negative correlation between antioxidant capacity and existence of a carcinogen in metabolism.
Batcioglu
Kadir
K
Inonu University, Institute of Health Sciences, Department of Biochemistry, Malatya, Turkey.
Ozturk
Cetin
C
Karagozler
Alev
A
Karatas
Fikret
F
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D012643:Selenium / Q000097:blood / Q000378:metabolism*
D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D012643:Selenium