Developmental changes and localization of mouse brain serine proteinase mRNA and protein in mouse brain.
Serine proteases are known to be involved in neural development and various functions in the central nervous system. Mouse brain serine proteinase (mBSP) is expressed almost exclusively in the mouse brain and it has been characterized at the molecular and biochemical levels. In this study, we analyzed the developmental changes and localization of mBSP mRNA and protein in the mouse brain, using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Expression of mBSP was strong in the white matter and the nerve tracts after postnatal day 30, especially in the cerebellum and the medulla oblongata. These results suggest that mBSP contributes to development and sustaining the functions in the mouse brain.
Yokoi
Takashi
T
Department of Pediatrics, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan. tyokoi@med.nagoya-cu.ac.jp
Yamamoto
Naoki
N
Tada
Toyohiro
T
Fujita
Masataka
M
Moriyama
Akihiko
A
Matsui
Hitoshi
H
Takahashi
Takayuki
T
Togari
Hajime
H
Kato
Taiji
T
Asai
Kiyofumi
K
D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D001923:Brain Chemistry / Q000235:genetics*; D004622:Embryo, Mammalian; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012697:Serine Endopeptidases