title: Interaction between P2Y and NMDA receptors in layer V pyramidal neurons of the rat prefrontal cortex.
abstract: In the first part of this study, monosynaptic excitatory postsynaptic potentials (EPSPs) in layer V of the rat prefrontal cortex were evoked by electrical stimulation of layer I. Recordings by intracellular sharp microelectrodes showed that EPSPs were concentration-dependently facilitated by the P2 receptor antagonistic ATP analogue 2-methylthio ATP (2-MeSATP), while ATP itself depressed the synaptic potentials. The inhibitory effect of ATP turned into facilitation in the presence of the adenosine A(1) receptor antagonist DPCPX. The 2-MeSATP-induced potentiation of EPSP amplitudes were prevented by the P2 receptor antagonists PPADS and Suramin. The EPSP was almost abolished by coapplication of the NMDA receptor antagonist AP-5 and the AMPA/kainate receptor antagonist CNQX. After blockade of the NMDA receptor-mediated part of the EPSP by AP-5, the stimulatory effect of 2-MeSATP disappeared. When NMDA or AMPA were pressure-applied onto pyramidal cells, only the NMDA-induced depolarization was potentiated by 2-MeSATP. In the second part of the study, NMDA-induced currents were measured by whole-cell patch-clamp pipettes. ATP, 2-MeSATP, UDP and UTP potentiated the response to NMDA, while ADP-beta-S was inactive. PPADS antagonized the effect of ATP. Synaptic isolation of pyramidal neurons by a Ca(2+)-free medium or tetrodotoxin did not alter the effect of ATP which, however, was markedly depressed when GTP in the micropipette was replaced by GDP-beta-S. These observations suggest that in layer V pyramidal neurons of the prefrontal cortex postsynaptically localized P2Y receptors interact with NMDA receptor-channels.
authors.affiliation: Rudolf-Boehm-Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstr. 16-18, D-04107, Leipzig, Germany. wirk@medizin.uni-leipzing.de
authors.forename: Kerstin
authors.initials: K
authors.lastname: Wirkner
authors.forename: Laszlo
authors.initials: L
authors.lastname: Köles
authors.forename: Susanne
authors.initials: S
authors.lastname: Thümmler
authors.forename: Julia
authors.initials: J
authors.lastname: Luthardt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Poelchen
authors.forename: Heike
authors.initials: H
authors.lastname: Franke
authors.forename: Susanna
authors.initials: S
authors.lastname: Fürst
authors.forename: Peter
authors.initials: P
authors.lastname: Illes
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D018048:Receptors, Purinergic P2; D016202:N-Methylaspartate; D000255:Adenosine Triphosphate