Nucleotide structure of the Scytalidium hyalinum and Scytalidium dimidiatum 18S subunit ribosomal RNA gene: evidence for the insertion of a group IE intron in the rDNA gene of S. dimidiatum.
The molds Scytalidium dimidiatum (Nattrassia mangiferae synanamorph) and Scytalidium hyalinum are responsible for dermatomycosis in humans. We sequenced their 18S subunit ribosomal RNA gene to identify these species with molecular biology-based methods. The coding sequences differed by a single polymorphism (A in S. dimidiatum, G in S. hyalinum). Moreover, we found an insert at position 1199 in the 18S rRNA gene sequence of S. dimidiatum. Its potential secondary structure was characteristic of a group IE intron. Bioinformatic and phylogenic group IE intron analyses generated four main homogeneous clusters. The S. dimidiatum intron is original and not related with other known IE group introns.
Machouart-Dubach
Marie
M
Laboratoire de Parasitologie-Mycologie, UFR Lariboisière Saint-Louis-Université Paris 7, Faculté de Médecine, 15 rue de l'école de médecine, 75006 Paris, France. paracord@wanadoo.fr
Lacroix
Claire
C
Vaury
Christelle
C
Feuilhade de Chauvin
Martine
M
Bellanné
Christine
C
Derouin
Francis
F
Lorenzo
Frédéric
F
D001203:Ascomycota / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000378:metabolism; D005798:Genes, Bacterial; D020459:Genes, rRNA; D007438:Introns; D003904:Mitosporic Fungi / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009711:Nucleotides / Q000032:analysis; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D016415:Sequence Alignment
D004275:DNA, Ribosomal; D009711:Nucleotides; D012337:RNA, Ribosomal, 18S