Importance of thymidine kinase activity for normal growth of lumpy skin disease virus (SA-Neethling).
In order to study the importance of an intact thymidine kinase (TK) gene for the vaccine strain of a southern African capripoxvirus, namely, lumpy skin disease virus (LSDV) (type SA-Neethling), a TK disruption recombinant was generated expressing the Escherichia coli beta-galactosidase (lacZ) reporter gene. A comparative growth study of the recombinant and wild-type (wt) LSDV in TK-positive primary and secondary cells and TK-negative secondary cells was performed. It was found that although recombinant and wt virus both grew in TK-positive cells without selection, the recombinant was unable to grow in TK-negative cells (with or without selection), indicating that TK activity is important, if not essential, for normal growth of LSDV.
Wallace
D B
DB
Applied Biotechnology Division, Onderstepoort Veterinary Institute, Pretoria, South Africa.
Viljoen
G J
GJ
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005822:Genetic Vectors; D007763:Lac Operon / Q000235:genetics / Q000502:physiology*; D008167:Lumpy skin disease virus / Q000235:genetics / Q000254:growth & development*; D011995:Recombination, Genetic; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism; D014614:Vaccines, Synthetic; D014765:Viral Vaccines
D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D013937:Thymidine Kinase