[Time-dependent and dose-dependent regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma by human insulin-like growth factor-I].
OBJECTIVE
We study the regulation of human progesterone receptor isoforms A and B in uterine endometrial carcinoma cell line by different concentration of human insulin-like growth factor-I (IGF-I) for different time, to investigate the roles of IGF-I and progesterone receptor isoforms in uterine endometrial carcinoma.


METHODS
The uterine endometrial adenocarcinoma cell line HEC-IB was cultured in vitro and the breast cancer cell line MCF-7 was used as control. Western blot was applied to examine the changes of the two isoforms by different concentration IGF-I for different time.


RESULTS
(1) In HEC-IB cell line, 10 ng/ml IGF-I made hPRB up-regulated in the first 24 h. But according to lager concentration and longer time, human progesterone receptor (hPR) B became down-regulated, which were significant at 20 ng/ml IGF-I for 72 h and 40 ng/ml IGF-I for 48 - 72 h. The change of hPRA was like hPRB. (2) In MCF-7 cell line, 10 ng/ml and 40 ng/ml IGF-I made hPRA and hPRB significantly up-regulated in 24, 48, 72 h. Twenty ng/ml IGF-I made hPRB up-regulated also in the first 24 h. But in 48 h and 72 h, down-regulation of hPRB was detected. Twenty ng/ml IGF-I made hPRA down-regulated in 24, 48, 72 h.


CONCLUSIONS
(1) The regulation of IGF-I to hPR isoforms has cell-type specific and dose-dependent and time-dependent. (2) In HEC-IB cell line, 10 ng/ml IGF-I made hPRB significantly up-regulated in 24 h. But following exposure to IGF-I at larger concentration and longer time, hPRB became down-regulated. The change of hPRA is like hPRB.
Zhang
Xiaohong
X
Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing 100044, China.
Wei
Lihui
L
Wang
Jianliu
J
Tu
Zheng
Z
D015153:Blotting, Western; D001943:Breast Neoplasms / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D009928:Organ Specificity / Q000502:physiology; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D011980:Receptors, Progesterone; C101118:progesterone receptor A; C097314:progesterone receptor B; D007334:Insulin-Like Growth Factor I