title: Clinical estimation of vancomycin measurement method on hemodialysis patient.
abstract: The fluorescence polarization immunoassay (FPIA) method is used to perform measurement of vancomycin hydrochloride (VCM) at many institutions. However, the values measured by the FPIA method are more vulnerable to overestimation than the high performance liquid chromatography (HPLC) method. In particular, it was not reported to perform exact therapeutic drug monitoring (TDM) measurement. Since overestimation is likely in patients with renal dysfunction, the HPLC method is preferable for TDM measurement. This study investigates the clinical conditions that lead to overestimation in the FPIA method, paying attention to the relation of clinical laboratory data inspection values and the existence of hemodialysis (HD). Overestimation in the evaluation of TDM using the FPIA method was clinically examined with 116 serum samples obtained from 18 cases medicated with VCM. The relevance between overestimation of patients who had not had HD performed was 72.7 +/- 61.7% (means +/- SD). In short, the overestimation was greatest in HD patients. Since overestimation did not affect the evaluation of clinical TDM, such as an effect and a side-effect, the TDM of VCM was shown to be satisfactorily evaluated by the simple FPIA method.
authors.affiliation: Department of Pharmacy, Kanto Medical Center NTT EC, Japan, 5-9-22, Higashigotanda, Shinagawa-ku, Tokyo 141-8625, Japan. tanaka@kmc.mhc.east.ntt.co.jp
authors.forename: Masayo
authors.initials: M
authors.lastname: Tanaka
authors.forename: Takao
authors.initials: T
authors.lastname: Orii
authors.forename: Tomoko
authors.initials: T
authors.lastname: Gomi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Motoko
authors.initials: M
authors.lastname: Kanke
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hirono
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D014640:Vancomycin / Q000097:blood*
chemical_list: D014640:Vancomycin