title: Characterization of the calcium-dependent proteolytic system in a mouse muscle cell line.
abstract: Many studies have demonstrated that the calcium-dependent proteolytic system (calpains and calpastatin) is involved in myoblast differentiation. It is also known that myogenic differentiation can be studied in vitro. In the present experiments, using a mouse muscle cell line (C2C12) we have analyzed both the sequences of appearance and the expression profiles of calpains 1, 2, 3 and calpastatin during the course of myoblast differentiation. Our results mainly show that the expression of ubiquitous calpains (calpain 1 and 2) and muscle-specific calpain (calpain 3) at the mRNAs level as well as at the protein level do not change significantly all along this biological process. In the same time, the specific inhibitor of ubiquitous calpains, calpastatin, presents a stable expression at mRNAs level as well as protein level, all along myoblast to myotube transition. A comparison with other myogenic cells is presented.
authors.affiliation: Laboratoire de Biochimie et Technologie des Aliments, ISTAB, USC-INRA 429, Université Bordeaux I, Talence, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Dargelos
authors.forename: Stephane
authors.initials: S
authors.lastname: Dedieu
authors.forename: Catherine
authors.initials: C
authors.lastname: Moyen
authors.forename: Sylvie
authors.initials: S
authors.lastname: Poussard
authors.forename: Philippe
authors.initials: P
authors.lastname: Veschambre
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Brustis
authors.forename: Patrick
authors.initials: P
authors.lastname: Cottin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002154:Calpain / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D024510:Muscle Development; D009132:Muscles / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D002135:Calcium-Binding Proteins; D012333:RNA, Messenger; C033668:calpastatin; D002154:Calpain; D002118:Calcium