[Adhesion induces matrix metalloproteinase-9 gene expression in ovarian cancer cells].
OBJECTIVE
This work was conducted to investigate the expression of matrix metalloproteinase 9 (MMP 9) gene in cancer cells by fibronectin adhesion and the underlying mechanism of cell invasion.


METHODS
Following adhesion of ovarian cancer cells A2780 to fibronectin, mRNA expression of MMP cells were assayed by reverse transcription-polymerase chain reaction (RT-PCR). MMP9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells.


RESULTS
Adhesion induced the increase of cellular MMP9 mRNA content in A2780 cells, not affecting the expression of MMP2 or TIMP-1 gene. The stimulation was enhanced with the increase adhesion time. When the transfected cells were allowed to adhere and spread on FN-coated surface, the promoter activity of MMP9 gene was also enhanced dramatically.


CONCLUSIONS
Cell-ECM adhesion may stimulate the expression of MMP9 gene through stimulating the promoter activity, thereby enhancing cancer cell invasion and metastasis.
Yan
Chunhong
C
Department of Tumor Molecular Biology, Reseach Clinic, Academy of Military Medical Sciences, Beijing 100039, China.
Tian
Fang
F
Xiao
Fengjun
F
Li
Keqin
K
Li
Chunhai
C
D002448:Cell Adhesion / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D020780:Matrix Metalloproteinase 9