DNA profiling: a valuable tool for quality control of sample logistics including occurrences of suspected sample confusion in a blood donation centre.
OBJECTIVE
A molecular method for analysing whole-blood samples should be established for quality control of plasma sample logistics.


METHODS
DNA profiles of retention samples (plasma) were compared to profiles of recent donations (whole blood). DNA extraction, amplification and detection were performed using the Qiagen DNA Blood Mini kit, the AmpFFISTR Profiler Plus Kit and capillary electrophoresis, respectively.


RESULTS
Matched pairs of full profiles were obtained for all samples investigated, therefore no deviation from the standardized procedures was detected.


CONCLUSIONS
Modified extraction and amplification protocols enabled DNA profiling to be used for the quality control of plasma samples. Hence, DNA profiling can be used in the blood bank as a safe and easy method for quality control of sample logistics.
Glock
B
B
Blood Donation Centre of the Austrian Red Cross for Vienna, Lower Austria. glock@redcross.or.at
Reisacher
R B K
RB
Schöck
M A
MA
Rogado
J
J
Feinböck
C
C
Eschner
G
G
Eder
E
E
Mayr
W R
WR
D001771:Blood Banks / Q000592:standards*; D001782:Blood Donors; D004247:DNA / Q000145:classification; D016172:DNA Fingerprinting; D019075:Electrophoresis, Capillary; D005784:Gene Amplification; D006801:Humans; D016133:Polymerase Chain Reaction; D011786:Quality Control; D012680:Sensitivity and Specificity; D013048:Specimen Handling
D004247:DNA