title: Structural basis of translational control by Escherichia coli threonyl tRNA synthetase.
abstract: Escherichia coli threonyl-tRNA synthetase (ThrRS) represses the translation of its own messenger RNA by binding to an operator located upstream of the initiation codon. The crystal structure of the complex between the core of ThrRS and the essential domain of the operator shows that the mRNA uses the recognition mode of the tRNA anticodon loop to initiate binding. The final positioning of the operator, upon which the control mechanism is based, relies on a characteristic RNA motif adapted to the enzyme surface. The finding of other thrS operators that have this conserved motif leads to a generalization of this regulatory mechanism to a subset of Gram-negative bacteria.
authors.affiliation: Laboratoire de Biologie et Génomique Structurales, IGBMC, BP163, 67404 Illkirch Cedex, France.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Torres-Larios
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Dock-Bregeon
authors.forename: Pascale
authors.initials: P
authors.lastname: Romby
authors.forename: Bernard
authors.initials: B
authors.lastname: Rees
authors.forename: Rajan
authors.initials: R
authors.lastname: Sankaranarayanan
authors.forename: Joel
authors.initials: J
authors.lastname: Caillet
authors.forename: Mathias
authors.initials: M
authors.lastname: Springer
authors.forename: Chantal
authors.initials: C
authors.lastname: Ehresmann
authors.forename: Bernard
authors.initials: B
authors.lastname: Ehresmann
authors.forename: Dino
authors.initials: D
authors.lastname: Moras
mesh_terms: D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D013914:Threonine-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012343:RNA, Transfer; D013914:Threonine-tRNA Ligase