title: Dolichol phosphate mannose synthase is differentially expressed in male and female worms of Schistosoma mansoni.
abstract: The cDNA encoding the Schistosoma mansoni dolichol phosphate mannose synthase was completely sequenced, displaying the highest homology with Cricetulus griseus and Saccharomyces pombe genes. The Schistosome enzyme had a K(m) of 0.127 microM, a value that is within the range of those reported for several other species. Thin-layer chromatography of the radiolabelled schistosome lipid intermediate showed it was identical to dolichol-phosphate (C80-C105). Expression of dolichol phosphate mannose synthase of S. mansoni (SmDPMS) was analysed by Northern blot and quantified by semi-quantitative RT-PCR with cDNA from mature and immature male and female worms. Northern blot analysis revealed a single 1-kb band. Both approaches confirmed a higher level of expression in mature female worms, as compared to immature and male worms.
authors.affiliation: Departamento de Medicina Tropical, Laboratório de Hanseníase, Fundação Oswaldo Cruz Avenida Brasil 4365 CEP 21045-900, Rio de Janeiro, Brazil.
authors.forename: A J
authors.initials: AJ
authors.lastname: Tempone
authors.forename: D R
authors.initials: DR
authors.lastname: Furtado
authors.forename: E R T
authors.initials: ER
authors.lastname: Gimba
authors.forename: F M B
authors.initials: FM
authors.lastname: Oliveira
authors.forename: F D
authors.initials: FD
authors.lastname: Rumjanek
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002855:Chromatography, Thin Layer; D017931:DNA Primers / Q000737:chemistry; D004288:Dolichol Phosphates / Q000378:metabolism*; D005260:Female; D015723:Gene Library; D008297:Male; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D004288:Dolichol Phosphates; D012333:RNA, Messenger; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase