title: Tumour necrosis factor-alpha receptors are present in the corpus luteum throughout the oestrous cycle and during the early gestation period in pigs.
abstract: To determine the physiological significance of tumour necrosis factor-alpha (TNFalpha) in the regulation of luteal functions in pig, this study was conducted to identify the presence of functional TNFalpha receptors in porcine corpora lutea (CL) throughout the oestrous cycle and the early gestation. The CL were isolated from pigs on days 4, 6, 8, 12 or 15 of the oestrous cycle (n=3; day 0 = oestrus) and days 15, 20 or 25 of gestation (n=3; day 0 = mating). A Scatchard analysis revealed the presence of a high-affinity binding site for TNFalpha in all samples (dissociation constant; 2.7 +/- 0.51 to 5.8 +/- 0.50 nM). The concentration of TNFalpha receptors was higher on day 15 of the oestrous cycle than on days 4 and 8 of the oestrous cycle (p < 0.05). Furthermore, TNFalpha receptor concentrations in the CL on days 15, 20 and 25 of gestation were significantly lower than on day 15 of the oestrous cycle (p < 0.05). On day 9 of the oestrous cycle, exposure of cultured luteal cells to 0.06-60 nM TNFalpha stimulated prostaglandin (PG) F2alpha and PGE2 secretion in a dose-dependent manner (p < 0.05). These results indicate that functional TNFalpha receptors are present in the porcine CL throughout the oestrous cycle and early gestation, and suggest that TNFalpha plays one or more physiological roles in regulating CL function throughout the oestrous cycle and the early gestation period. In addition, TNFalpha receptor concentration in the CL of the late luteal stage (day 15) of the oestrous cycle was higher than on the respective day in the early pregnant pig, suggesting that TNFalpha plays a role in accomplishing luteolysis in the porcine CL.
authors.affiliation: Laboratory of Reproductive Endocrinology, Faculty of Agriculture, Okayama University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyamoto
authors.forename: R
authors.initials: R
authors.lastname: Sakumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Sakabe
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: A
authors.initials: A
authors.lastname: Okano
authors.forename: K
authors.initials: K
authors.lastname: Okuda
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D003338:Corpus Luteum / Q000737:chemistry* / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D030762:Estrous Cycle; D005260:Female; D005865:Gestational Age; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011869:Radioligand Assay; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis* / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D015237:Dinoprost; D015232:Dinoprostone