title: Precision and functional specificity in mRNA decay.
abstract: Posttranscriptional processing of mRNA is an integral component of the gene expression program. By using DNA microarrays, we precisely measured the decay of each yeast mRNA, after thermal inactivation of a temperature-sensitive RNA polymerase II. The half-lives varied widely, ranging from approximately 3 min to more than 90 min. We found no simple correlation between mRNA half-lives and ORF size, codon bias, ribosome density, or abundance. However, the decay rates of mRNAs encoding groups of proteins that act together in stoichiometric complexes were generally closely matched, and other evidence pointed to a more general relationship between physiological function and mRNA turnover rates. The results provide strong evidence that precise control of the decay of each mRNA is a fundamental feature of the gene expression program in yeast.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.
authors.forename: Yulei
authors.initials: Y
authors.lastname: Wang
authors.forename: Chih Long
authors.initials: CL
authors.lastname: Liu
authors.forename: John D
authors.initials: JD
authors.lastname: Storey
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Tibshirani
authors.forename: Daniel
authors.initials: D
authors.lastname: Herschlag
authors.forename: Patrick O
authors.initials: PO
authors.lastname: Brown
mesh_terms: D006019:Glycolysis; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis; D011061:Poly A / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D013696:Temperature; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012319:RNA Polymerase II