title: Transplantation of Descemet's membrane carrying viable endothelium through a small scleral incision.
abstract: OBJECTIVE
  To design a technique for transplantation of the Descemet's membrane (DM) as a carrier for its endothelium, while maintaining the low-astigmatic recipient anterior corneal curvature.
  
  
  METHODS
  In a human eye bank eye model, recipient eyes (n = 15) had a 5.0-mm scleral tunnel incision made, extending 1.0 mm into the peripheral cornea. A 9.0-mm-diameter Descemeto rhexis was created, i.e., a circular portion of DM was stripped from the posterior stroma. With use of a custom-made inserter, a 9.0-mm-diameter donor DM carrying autologous donor endothelium was brought into the anterior chamber and positioned against the recipient posterior stroma. The procedure was evaluated by keratometry, endothelial vital and supravital staining, and light microscopy.
  
  
  RESULTS
  Mean postoperative astigmatism was 1.0 D (+/-0.6 D). Implanted donor DM showed an intact endothelial cell layer, with 3.4% (+/-1.1%) dispersed focal cell death. Microscopy showed normal endothelial cell morphology and complete apposition of the donor DM against the recipient posterior stroma.
  
  
  CONCLUSIONS
  DM can be transplanted in vitro with acceptable damage to the donor endothelium and with little induced astigmatism.
authors.affiliation: Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands. melles@niioc.nl
authors.forename: Gerrit R J
authors.initials: GR
authors.lastname: Melles
authors.forename: Frank
authors.initials: F
authors.lastname: Lander
authors.forename: Frank J R
authors.initials: FJ
authors.lastname: Rietveld
mesh_terms: D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D016039:Corneal Transplantation; D003886:Descemet Membrane / Q000502:physiology*; D004728:Endothelium, Corneal / Q000637:transplantation*; D006801:Humans; D012590:Sclera / Q000601:surgery*; D013524:Surgical Flaps