A glucose biosensor based on microporous polyacrylonitrile synthesized by single rare-earth catalyst.
A glucose biosensor was constructed by electrochemical adsorption of glucose oxidase (GOD) in microporous polyacrylonitrile (PAN), which was synthesized by single rare-earth catalyst-Y(OAr)(3.) The biosensor shows a fast response, good operational stability and reproducibility. The influences of molecular weight of polymer, pH, operating potential and temperature on the biosensor response were explored by amperometric method. The apparent activation energy and optimum pH of enzyme-catalyzed reaction are 35.9 kJ mol(-1) and 6.2, respectively.
Zheng
Hao
H
Institute of Polymer Science, Zhejiang University, Hangzhou 310027, People's Republic of China.
Xue
Huaiguo
H
Zhang
Yifeng
Y
Shen
Zhiquan
Z
D000180:Acrylic Resins / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D001786:Blood Glucose / Q000032:analysis; D004563:Electrochemistry; D004574:Electromagnetic Fields; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D008674:Metals, Rare Earth / Q000737:chemistry; D008958:Models, Molecular; D008970:Molecular Weight; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D000180:Acrylic Resins; D001786:Blood Glucose; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D008674:Metals, Rare Earth; C010504:polyacrylonitrile; D005949:Glucose Oxidase; D005947:Glucose