title: Developmental changes and localization of mouse brain serine proteinase mRNA and protein in mouse brain.
abstract: Serine proteases are known to be involved in neural development and various functions in the central nervous system. Mouse brain serine proteinase (mBSP) is expressed almost exclusively in the mouse brain and it has been characterized at the molecular and biochemical levels. In this study, we analyzed the developmental changes and localization of mBSP mRNA and protein in the mouse brain, using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Expression of mBSP was strong in the white matter and the nerve tracts after postnatal day 30, especially in the cerebellum and the medulla oblongata. These results suggest that mBSP contributes to development and sustaining the functions in the mouse brain.
authors.affiliation: Department of Pediatrics, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan. tyokoi@med.nagoya-cu.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoi
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Tada
authors.forename: Masataka
authors.initials: M
authors.lastname: Fujita
authors.forename: Akihiko
authors.initials: A
authors.lastname: Moriyama
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Matsui
authors.forename: Takayuki
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hajime
authors.initials: H
authors.lastname: Togari
authors.forename: Taiji
authors.initials: T
authors.lastname: Kato
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Asai
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D001923:Brain Chemistry / Q000235:genetics*; D004622:Embryo, Mammalian; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012697:Serine Endopeptidases