[Study of encapsulation of suicide gene in poly(D,L-lactic-co-glycolic acid) nanoparticles and its characteristics in vitro].
We constructed the AFP promotor, suicide gene and EGFP eukaryotic expression vector recombinant plasmid, and this plasmid DNA was encapsulated by biodegradable, biocompatible polymer PLGA to prepare nanoparticles. The results demonstrate that the mean diameter of DNA-PLGA-NP is 68 nm, the encapsulation ratio reaches to 80%, and the PLGA nanoparticles can protect plasmid DNA from digestion by deoxyribonuclease I (DNaseI) and sonication-induced shearing in vitro.
He
Qin
Q
School of Pharmacy, Sichuan University, Chengdu 610041.
Zhang
Zhirong
Z
Liu
Ji
J
Shu
Min
M
Zhang
Hua
H
Lu
Yi
Y
Nie
Fei
F
Luo
Peng
P
D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D004247:DNA / Q000008:administration & dosage*; D005822:Genetic Vectors; D066298:In Vitro Techniques; D019344:Lactic Acid; D010316:Particle Size; D010957:Plasmids / Q000235:genetics; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D011995:Recombination, Genetic
D001672:Biocompatible Materials; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA