Functional analysis of the 5'-flanking region of the human alanine:glyoxylate aminotransferase gene AGXT.
Primer extension of human liver poly(A)(+) RNA revealed that the main transcription start site of the human alanine:glyoxylate aminotransferase gene (AGXT) is situated near 45 bp upstream from the translation start site. Deletion analysis using the 1203 bp 5'-flanking region of the AGXT gene and a luciferase reporter suggested that the promoter sequence is most likely located 2-325 bp upstream from the translation start site, possibly with enhancer elements 440-700 bp upstream. It was also suggested that the region -2 to -64 is important for the expression of the AGXT gene. The region -2 to -325 has two TATA boxes and some initiator elements.
Sato
Manabu
M
Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama, Japan. manasato@med.kawasaki-m.ac.jp
Toné
Shigenobu
S
Ishikawa
Tetsuya
T
Purdue
P Edward
PE
Danpure
Christopher J
CJ
Minatogawa
Yohsuke
Y
D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D018076:DNA, Complementary / Q000737:chemistry; D017930:Genes, Reporter; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D000637:Transaminases / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*
D018076:DNA, Complementary; D000637:Transaminases; C019635:Alanine-glyoxylate transaminase