title: Evaluation of relationship between chromosome 22 and p53 gene alterations and the subtype of meningiomas by the interphase-FISH technique.
abstract: In this study, we investigated the relationship between genetic alterations such as chromosome 22 aneuploidy and p53 gene deletion, and the pathological types of meningioma of typical and aggressive forms. Thirty-four meningiomas (23 typical and 11 aggressive) were examined by application of fluorescence in situ hybridization (FISH) with chromosome 22 specific alpha satellite probe and a combination of p53 locus specific and chromosome 17 centromere specific alpha satellite probes, to evaluate the chromosome 22 aneuploidy and gain or loss of p53 gene along with chromosome 17. The results showed that, although chromosome 22 aneuploidy was seen in 7 out of 23 typical (30.4%) and 4 out of 11 aggressive meningiomas (36.3%), no p53 deletion was detected in typical meningiomas, and p53 deletion was detected in 3 out of 11 aggressive meningiomas (1 atypical and 2 malignant), which had recurrence. There were no simultaneous occurrences of p53 gene deletions between typical and aggressive meningiomas. The present findings indicate that the loss of chromosome 22 may be involved with tumorogenesis of typical and aggressive meningiomas, while p53 gene deletions may be involved with malignant progression and recurrence in the aggressive meningiomas.
authors.affiliation: Department of Medical Biology and Genetics, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Tahsin
authors.initials: T
authors.lastname: Yakut
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bekar
authors.forename: Muammer
authors.initials: M
authors.lastname: Doygun
authors.forename: Hasan
authors.initials: H
authors.lastname: Acar
authors.forename: Unal
authors.initials: U
authors.lastname: Egeli
authors.forename: Erhan
authors.initials: E
authors.lastname: Ogul
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D001932:Brain Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D005260:Female; D017353:Gene Deletion; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D009006:Monosomy; D009154:Mutation; D010641:Phenotype