title: Identification and functional analysis of an immunoreactive DsbA-like thio-disulfide oxidoreductase of Ehrlichia spp.
abstract: Novel homologous DsbA-like disulfide bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which restored DsbA activity in complemented Escherichia coli dsbA mutants. Recombinant Ehrlichia Dsb (eDsb) proteins were recognized by sera from E. canis-infected dogs but not from E. chaffeensis-infected patients. The eDsb proteins were observed primarily in the periplasm of E. chaffeensis and E. canis.
authors.affiliation: Department of Pathology and WHO Collaborating Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA. jemcbrid@utmb.edu
authors.forename: Jere W
authors.initials: JW
authors.lastname: McBride
authors.forename: Lucy M
authors.initials: LM
authors.lastname: Ndip
authors.forename: Vsevolod L
authors.initials: VL
authors.lastname: Popov
authors.forename: David H
authors.initials: DH
authors.lastname: Walker
mesh_terms: D000818:Animals; D001483:Base Sequence; D004285:Dogs; D004536:Ehrlichia / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases / Q000032:analysis* / Q000235:genetics / Q000502:physiology
chemical_list: D019704:Protein Disulfide-Isomerases