Characterization of a class II defective transposon carrying two haloacetate dehalogenase genes from Delftia acidovorans plasmid pUO1.
The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-related transposons. TnHad2 was defective in transposition because of its lacking the transposase and resolvase genes. TnHad2 could transpose when the Tn21-encoded transposase and resolvase were supplied in trans. These results demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposon.
Sota
Masahiro
M
Department of Environmental Simulation, Institute for Environmental Sciences, Obuchi, Rokkasho, Aomori 039-3212, Japan. sota@ies.or.jp
Endo
Masahiro
M
Nitta
Keiji
K
Kawasaki
Haruhiko
H
Tsuda
Masataka
M
D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D045564:Transposon Resolvases
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C473490:Tn21 resolvase; D045564:Transposon Resolvases; D006867:Hydrolases; C023098:haloacetate dehalogenase