Nonenzymatic derived lipid peroxide, 8-iso-PGF2 alpha, participates in the pathogenesis of delayed cerebral vasospasm in a canine SAH model.
We studied whether 8-iso-PGF2alpha, nonenzymatic arachidonyl peroxide, participated in the pathogenesis of delayed vasospasm using a canine subarachnoid hemorrhage (SAH) model. Fourteen adult mongrel dogs were divided into two groups, two-hemorrhage SAH group (n = 8) and control group (n = 6). The contents of 8-iso-PGF2alpha in CSF, the basilar artery segment, and subarachnoid clot were measured by enzyme immunoassay kit. The CSF 8-iso-PGF2alpha content on Day 7 in the SAH group was 67.9+/-29.9 pg ml(-1) (n = 8), which was significantly higher than 27.1+/-13.8 (n = 8) on Day 0 in the SAH group, and 33.2+/-14.4 pg ml(-1) (n = 5) on Day 7 in the control group. The 8-iso-PGF2alpha content in the basilar artery segment with spasm on Day 7 in the SAH group was 13.5+/-1.9 pg mg(-1) wet weight (n = 8), significantly higher than 8.7+/-1.9 (n = 6) in the control group. The 8-iso-PGF2alpha content in subarachnoid clot was 1.7+/-1.4 ng g(-1) wet weight (n = 8). Significant elevation of the 8-iso-PGF2alpha contents in the CSF and the basilar artery segment occurred on Day 7 in the SAH group. The subarachnoid clot enclosed the basilar artery on Day 7, contained a considerable amount of 8-iso-PGF2alpha. These results suggested that 8-iso-PGF2alpha could play a crucial role in the pathogenesis of the delayed cerebral vasospasm.
Sakamoto
Makoto
M
Department of Neurosurgery, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago, Japan. sakamako@grape.med.tottori-u.ac.jp
Takaki
Eiichi
E
Yamashita
Keiichi
K
Watanabe
Kenji
K
Tabuchi
Sadaharu
S
Watanabe
Takashi
T
Satoh
Keisuke
K
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives*; D004285:Dogs; D028441:F2-Isoprostanes / Q000502:physiology*; D008054:Lipid Peroxides / Q000502:physiology*; D013345:Subarachnoid Hemorrhage / Q000150:complications* / Q000378:metabolism* / Q000503:physiopathology; D020301:Vasospasm, Intracranial / Q000209:etiology* / Q000378:metabolism* / Q000503:physiopathology
D028441:F2-Isoprostanes; D008054:Lipid Peroxides; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost