title: ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat.
abstract: In eukaryotic cells, secretion is achieved by vesicular transport. Fusion of such vesicles with the correct target compartment relies on SNARE proteins on both vesicle (v-SNARE) and the target membranes (t-SNARE). At present it is not clear how v-SNAREs are incorporated into transport vesicles. Here, we show that binding of ADP-ribosylation factor (ARF)-GTPase-activating protein (GAP) to ER-Golgi v-SNAREs is an essential step for recruitment of Arf1p and coatomer, proteins that together form the COPI coat. ARF-GAP acts catalytically to recruit COPI components. Inclusion of v-SNAREs into COPI vesicles could be mediated by direct interaction with the coat. The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding.
authors.affiliation: Friedrich Miescher Laboratory, Max Planck Society, D-72076 Tübingen, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Rein
authors.forename: Uwe
authors.initials: U
authors.lastname: Andag
authors.forename: Rainer
authors.initials: R
authors.lastname: Duden
authors.forename: Hans Dieter
authors.initials: HD
authors.lastname: Schmitt
authors.forename: Anne
authors.initials: A
authors.lastname: Spang
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D022181:COP-Coated Vesicles / Q000378:metabolism; D020755:Coat Protein Complex I / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins
chemical_list: D020755:Coat Protein Complex I; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C089147:GCS1 protein, S cerevisiae; D020690:GTPase-Activating Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors