[Overexpression of EDAG-1 in NIH3T3 cells leads to malignant transformation].
To study the biological function of EDAG-1, a novel gene cloned by our lab recently, the EDAG-1 cDNA was subcloned into pcDNA3. 1(+) vector and transfected into NIH3T3 cells by Lipofect AMINE methods. Overexpression of EDAG-1 in NIH3T3 cells resulted in loss of cell contact inhibition, anchorage-independent growth in soft agar and tumor formation in nude mice. These results suggest that EDAG-1 is a transforming gene and may be associated with the development of tumors, especially in hematopoietic system.
Lü
Jun
J
Institute of Infectious Disease, First Affiliated Hospital, Medical College of Zhejiang University, Hangzhou 710006, China.
Xu
Wang-Xiang
WX
Wang
Si-Ying
SY
Jiang
Yan
Y
Li
Chang-Yan
CY
Cai
Wei-Min
WM
Yang
Xiao-Ming
XM
D016475:3T3 Cells; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004195:Disease Models, Animal; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes / Q000502:physiology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000502:physiology*
D011506:Proteins