title: CD34 selection using three immunoselection devices: comparison of T-cell depleted allografts.
abstract: BACKGROUND
  T-cell depletion of allografts markedly reduces the incidence of GvHD following BMT. The approach taken at our Center has utilized the physical separation method of counterflow centrifugal elutriation (CCE), augmented by recovery of stem cells from lymphocyte-rich fractions by immunoaffinity selection of CD34(+) stem cells. We wanted to compare the performance characteristics of three commercially available selection devices, as well as the clinical outcomes of patients who received allografts engineered by the different devices.
  
  
  METHODS
  BM allografts were prepared for patients undergoing BMT for hematologic malignancies. BM cells were separated into lymphocyte-rich and lymphocyte-depleted fractions using CCE, followed by recovery of CD34(+) cells from the lymphocyte-rich fraction using one of three immunoselection devices [CellPro CEPRATE, Nexell Isolex 300i (software version 2.5) and AmCell CliniMACS]. Allografts consisted of the lymphocyte-depleted fraction plus the CD34-selected fraction.
  
  
  RESULTS
  Yields of CD34(+) cells were comparable for the three devices. However, there were significant differences in purity (CEPRATE < Isolex 300i < CliniMACS) and time from start of fractionation to infusion (CEPRATE < CliniMACS < Isolex 300i). More technical problems were encountered with the Isolex 300i device. Allograft compositions were comparable. Transplant outcomes (engraftment and incidence of GvHD) also were comparable.
  
  
  CONCLUSIONS
  Qualitatively and quantitatively, allografts prepared with the CEPRATE, Isolex 300i (v 2.5) and CliniMACS devices should be considered comparable for use in this setting and probably also for direct T-cell depletion of BM.
authors.affiliation: Division of Hematologic Malignancies, The Johns Hopkins Oncology Center Baltimore, MD, USA.
authors.forename: P V
authors.initials: PV
authors.lastname: O'Donnell
authors.forename: B
authors.initials: B
authors.lastname: Myers
authors.forename: J
authors.initials: J
authors.lastname: Edwards
authors.forename: K
authors.initials: K
authors.lastname: Loper
authors.forename: P
authors.initials: P
authors.lastname: Rhubart
authors.forename: S J
authors.initials: SJ
authors.lastname: Noga
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D016026:Bone Marrow Transplantation / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells; D006801:Humans; D008212:Lymphocyte Depletion; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes; D014184:Transplantation, Homologous
chemical_list: D018952:Antigens, CD34