Synthetic gene network for entraining and amplifying cellular oscillations.
We present a model for a synthetic gene oscillator and consider the coupling of the oscillator to a periodic process that is intrinsic to the cell. We investigate the synchronization properties of the coupled system, and show how the oscillator can be constructed to yield a significant amplification of cellular oscillations. We reduce the driven oscillator equations to a normal form, and analytically determine the amplification as a function of the strength of the cellular oscillations. The ability to couple naturally occurring genetic oscillations to a synthetically designed network could lead to possible strategies for entraining and/or amplifying oscillations in cellular protein levels.
Hasty
Jeff
J
Center for BioDynamics and Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA.
Dolnik
Milos
M
Rottschäfer
Vivi
V
Collins
James J
JJ
D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D007763:Lac Operon; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C016649:phage repressor proteins