Synthesis and spectroscopic studies of model red fluorescent protein chromophores.
[reaction: see text]. Here we describe the synthesis and spectroscopic characterization of two compounds designed to model the chromophore in DsRed, a red fluorescent protein. Comparison with model green fluorescent protein (GFP) chromophores indicates that the additional conjugation in the DsRed models can account, in part, for the red-shifted absorption and emission properties of DsRed compared to those of GFP. In contrast to the GFP models, the DsRed models are fluorescent with quantum yields of 0.002-0.01 in CHCl3.
He
Xiang
X
Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794-3400, USA.
Bell
Alasdair F
AF
Tonge
Peter J
PJ
D000215:Acylation; D000818:Animals; D003063:Cnidaria / Q000737:chemistry; D003437:Crotonates / Q000737:chemistry; D008164:Luminescent Proteins / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D000097573:Red Fluorescent Protein
D003437:Crotonates; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein