title: Individual cases of autochthonous malaria in Evros Province, northern Greece: serological aspects.
abstract: From 1994 to 1995 four presumably autochthonous malaria cases were diagnosed by blood smear microscopy in Evros Province, northern Greece. Alarmed by these unexpected infections a serological survey was performed from 1997 to 1999 in ten rural villages, including those where the malaria cases had occurred. Among the 1,102 blood samples examined, nine turned out to contain specific antibodies against plasmodial parasites as detected by indirect fluorescent antibody test, including two of the former patients. The remaining seven samples were taken from healthy individuals with no history of recent infection or of having travelled to endemic areas. A further 21 sera showed borderline reactivity with Plasmodium falciparum antigen. Although no retrospective examination of the blood specimens could be performed to confirm the serological results by direct parasite detection, we can conclude that at least the seropositive persons have actually undergone infection with malaria parasites but developed no or only mild clinical symptoms which went unnoticed. It is becoming obvious that even in European countries where climatic and vector conditions are favourable for the development of the parasite there is a potential risk of incidental malaria transmission by indigenous Anopheles mosquitoes.
authors.affiliation: Institute for Medical Parasitology, University of Bonn, Germany.
authors.forename: Helge
authors.initials: H
authors.lastname: Kampen
authors.forename: Efstratios
authors.initials: E
authors.lastname: Maltezos
authors.forename: Maria
authors.initials: M
authors.lastname: Pagonaki
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Hunfeld
authors.forename: Walter A
authors.initials: WA
authors.lastname: Maier
authors.forename: Hanns M
authors.initials: HM
authors.lastname: Seitz
mesh_terms: D000818:Animals; D019084:Fluorescent Antibody Technique, Indirect; D005843:Geography; D006115:Greece; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification; D008288:Malaria / Q000145:classification* / Q000175:diagnosis; D016778:Malaria, Falciparum / Q000097:blood / Q000145:classification* / Q000175:diagnosis; D010963:Plasmodium falciparum / Q000302:isolation & purification*