Proton transfers in the photochemical reaction cycle of proteorhodopsin.
The spectral and photochemical properties of proteorhodopsin (PR) were determined to compare its proton transport steps to those of bacteriorhodopsin (BR). Static and time-resolved measurements on wild-type PR and several mutants were done in the visible and infrared (FTIR and FT-Raman). Assignment of the observed C=O stretch bands indicated that Asp-97 and Glu-108 serve as the proton acceptor and donor, respectively, to the retinal Schiff base, as do the residues at corresponding positions in BR, but there are numerous spectral and kinetic differences between the two proteins. There is no detectable dark-adaptation in PR, and the chromophore contains nearly entirely all-trans retinal. Because the pK(a) of Asp-97 is relatively high (7.1), the proton-transporting photocycle is produced only at alkaline pH. It contains at least seven transient states with decay times in the range from 10 micros to 200 ms, but the analysis reveals only three distinct spectral forms. The first is a red-shifted K-like state. Proton release does not occur during the very slow (several milliseconds) rise of the second, M-like, intermediate, consistent with lack of the residues facilitating extracellular proton release in BR. Proton uptake from the bulk, presumably on the cytoplasmic side, takes place prior to release (tau approximately 2 ms), and coincident with reprotonation of the retinal Schiff base. The intermediate produced by this process contains 13-cis retinal as does the N state of BR, but its absorption maximum is red-shifted relative to PR (like the O state of BR). The decay of this N-like state is coupled to reisomerization of the retinal to all-trans, and produces a state that is O-like in its C-C stretch bands, but has an absorption maximum apparently close to that of unphotolyzed PR.
Dioumaev
Andrei K
AK
Department of Physiology & Biophysics, University of California, Irvine, California 92697, USA.
Brown
Leonid S
LS
Shih
Jennifer
J
Spudich
Elena N
EN
Spudich
John L
JL
Lanyi
Janos K
JK
D001436:Bacteriorhodopsins / Q000737:chemistry; D020563:Gammaproteobacteria / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D010777:Photochemistry / Q000379:methods; D017494:Proton Pumps / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry* / Q000235:genetics; D025604:Rhodopsins, Microbial; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
D017494:Proton Pumps; D025604:Rhodopsins, Microbial; C413548:proteorhodopsin; D001436:Bacteriorhodopsins; D012243:Rhodopsin