[A new approach to the isolation of genomic DNA samples from yeast and fungi: preparation of DNA-containing cell envelopes and their use in PCR].
A simple and rapid procedure for the preparation of yeast and fungal DNA samples useful in PCR amplification was developed. The DNA was purified from proteins, lipids, polysaccharides, and other impurities by their high-temperature extraction (in a boiling water bath) with buffer solutions containing chaotropic salts. Under these conditions, yeast and fungal cell envelopes remain unbroken and retain the original DNA and RNA that could be used for direct PCR amplification. We called the proposed PCR technique as the PCR using DNA-containing cell envelopes.
Danilevich
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VN
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, GSP Moscow, 117997 Russia. dan@mail.ibch.ru
Grishin
E V
EV
D001671:Biochemistry / Q000379:methods; D002021:Buffers; D002462:Cell Membrane / Q000737:chemistry / Q000235:genetics*; D004271:DNA, Fungal / Q000302:isolation & purification*; D005658:Fungi / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015003:Yeasts / Q000235:genetics
D002021:Buffers; D004271:DNA, Fungal