Induction of protective immunity to Listeria monocytogenes by immunization with plasmid DNA expressing a helper T-cell epitope that replaces the class II-associated invariant chain peptide of the invariant chain.
Listeria epitope-specific helper T (Th) cells were able to be primed and induced in vivo by immunization with a plasmid carrying an invariant chain (Ii) gene whose class II-associated invariant chain peptide (CLIP) region was replaced by a Listeria Th epitope. Immunization of C3H/He mice with an Ii-LLO 215-226 plasmid induced specific interferon-gamma- and interleukin 2-producing Th cells and conferred significant protective immunity against listerial infection.
Nagata
Toshi
T
Department of Microbiology and Immunology, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan. tnagata@hama-med.ac.jp
Aoshi
Taiki
T
Suzuki
Mina
M
Uchijima
Masato
M
Kim
Yeung-Hyen
YH
Yang
Zhibo
Z
Koide
Yukio
Y
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology; D006460:Hemolysin Proteins; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010446:Peptide Fragments; D019444:Vaccines, DNA; C045084:invariant chain; D007371:Interferon-gamma; C038705:hlyA protein, Listeria monocytogenes