title: Improved polygalacturonase production from Bacillus sp. MG-cp-2 under submerged (SmF) and solid state (SSF) fermentation.
abstract: OBJECTIVE
  To investigate the effect of amino acids, vitamins and surfactants on polygalacturonase production from Bacillus sp. MG-cp-2 under submerged (SmF) and solid state fermentation (SSF).
  
  
  RESULTS
  Bacillus sp. MG-cp-2 was isolated from the outer covering of the seeds of Celastrus paniculatus. Out of the various surfactants, amino acids and vitamins, Tween-60, DL-serine and folic acid maximally enhanced polygalacturonase production by 2.7-fold (240.0 U x ml(-1)), 4.0-fold (360.0 U x ml(-1)) and 3.8-fold (342.0 U x ml(-1)) respectively, under submerged fermentation (SmF). In solid state fermentation (SSF), Tween-80, pyridoxine and DL-ornithine monohydrochloride induced highest enzyme production up to 1.73-fold (6956.5 U x g(-1)), 5.3-fold (21224.4 U x g(-1)) and 5.74-fold (23076.9 U x g(-1)), respectively.
  
  
  CONCLUSIONS
  Amino acids and their analogues, vitamins and surfactants effect significantly polygalacturonase production by Bacillus sp. MG-cp-2 when grown under submerged (SmF) and solid state fermentation (SSF) conditions.
  
  
  CONCLUSIONS
  The study provides useful information about regulation of polygalacturonase biosynthesis in Bacillus sp. MG-cp-2, which appears to be an interplay of nutritional and physical factors. Alkaline polygalacturonase from Bacillus sp. MG-cp-2 will be extremely useful in the treatment of alkaline pectic waste waters from vegetable and fruit processing industries and in degumming of bast fibres.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India.
authors.forename: M
authors.initials: M
authors.lastname: Kapoor
authors.forename: R C
authors.initials: RC
authors.lastname: Kuhad
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D001407:Bacillus / Q000201:enzymology*; D019149:Bioreactors; D005285:Fermentation; D011096:Polygalacturonase / Q000096:biosynthesis*; D013501:Surface-Active Agents / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D013501:Surface-Active Agents; D014815:Vitamins; D011096:Polygalacturonase