Assessment of the ability to model proteins with leucine-rich repeats in light of the latest structural information.
The three-dimensional structures of leucine-rich repeat (LRR)-containing proteins from five different families were previously predicted based on the crystal structure of the ribonuclease inhibitor, using an approach that combined homology-based modeling, structure-based sequence alignment of LRRs, and several rational assumptions. The structural models have been produced based on very limited sequence similarity, which, in general, cannot yield trustworthy predictions. Recently, the protein structures from three of these five families have been determined. In this report we estimate the quality of the modeling approach by comparing the models with the experimentally determined structures. The comparison suggests that the general architecture, curvature, "interior/exterior" orientations of side chains, and backbone conformation of the LRR structures can be predicted correctly. On the other hand, the analysis revealed that, in some cases, it is difficult to predict correctly the twist of the overall super-helical structure. Taking into consideration the conclusions from these comparisons, we identified a new family of bacterial LRR proteins and present its structural model. The reliability of the LRR protein modeling suggests that it would be informative to apply similar modeling approaches to other classes of solenoid proteins.
Kajava
Andrey V
AV
Center for Molecular Modeling, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892, USA. kajava@helix.nih.gov
Kobe
Bostjan
B
D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins; D005656:Fungal Proteins / Q000737:chemistry*; D020690:GTPase-Activating Proteins / Q000737:chemistry*; D007930:Leucine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011487:Protein Conformation; D020449:Repetitive Sequences, Amino Acid; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; C066843:sds22 protein, S pombe; D007930:Leucine