Kinetics of 5-enolpyruvylshikimate-3-phosphate synthase inhibition by glyphosate.
The herbicide glyphosate (N-phosphonomethyl glycine) is a potent reversible inhibitor of the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase activity of the purified arom multienzyme complex from Neurospora crassa. Inhibition of the EPSP synthase reaction by glyphosate is competitive with respect to phosphoenolpyruvate, with K(i) 1.1 microM, and uncompetitive with respect to shikimate-3-phosphate. The kinetic patterns are consistent with a compulsory order sequential mechanism in which either PEP or glyphosate can bind to an enzyme: shikimate-3-phosphate complex.
Boocock
M R
MR
Department of Biochemistry, University of Glasgow, Scotland.
Coggins
J R
JR
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000037:antagonists & inhibitors*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007700:Kinetics; D009492:Neurospora crassa / Q000201:enzymology; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D000097797:Glyphosate
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D005998:Glycine; D012765:Shikimic Acid; D000097797:Glyphosate; C042786:shikimic acid-3-phosphate