Two new HLA class I alleles recognised by PCR sequence-specific primer and sequencing based typing: B*3805 and Cw*0408.
Two new HLA class I alleles have been recognised by molecular-based typing. B*3805 was initially identified by polymerase chain reaction using sequence-specific primers (PCR-SSP) and afterwards confirmed by sequencing based typing (SBT) studies in a Spanish Caucasian blood cord unit. A unique nucleotide change throughout exons 2, 3 and 4, leading to the amino acid replacement Ser11Ala, differentiates B*3801 and *3805. This position behaves as a dimorphic residue in HLA-B and -C loci, and seems to be structurally unrelated to peptide and TcR recognition. Cw*0408 was first detected by SBT in two African American bone marrow donors in combination with its most structurally related allele, Cw*04011. The single amino acid change found between Cw*04011 and Cw*0408 was Thr163Leu, a residue involved in pocket A of the peptide-binding cleft. This new allele could be the result of a gene conversion event between Cw*04011 and any of the Cw*03 alleles.
García-Sánchez
F
F
Laboratory of Histocompatibility, Regional Transfusion Centre, Madrid, Spain. crtscam@teleline.es
Fernández-De-Velasco
J
J
Lillo
R
R
Alvarez-Doval
A
A
Rodríguez
M A
MA
Aviles
M J
MJ
Balas
A
A
Vicario
J L
JL
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005805:Genes, MHC Class I; D015235:HLA-B Antigens / Q000235:genetics*; D059945:HLA-B38 Antigen; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D044465:White People / Q000235:genetics
D017931:DNA Primers; D015235:HLA-B Antigens; C466437:HLA-B*38:05 antigen; D059945:HLA-B38 Antigen; D015236:HLA-C Antigens; C466436:HLA-C*04:08 antigen