[Investigation on development potential of blastomeres isolated from 4-cell human embryos].
OBJECTIVE
To investigate the development potential of blastomeres isolated from 4-cell human embryos.


METHODS
Human oocytes were collected after controlled ovarian hyperstimulation and fertilized in vitro. The zona pellucida of 4-cell stage embryos were removed and the embryos were separated to single blastomeres and cultured in vitro. The development and differentiation of the blastomeres were observed.


RESULTS
83 blastomeres were isolated from 20 4-cell and 2 3-cell human embryos. Of those, 35 developed to expanded blastocyst.


CONCLUSIONS
Blastomeres isolated from the 4-cell human embryos have the competence to develop to expanded blastocyst.
Gao
Shiyou
S
Reproductive Medicine Center, Jinan Central Railway Hospital, Jinan 250001, China.
Sun
Wei
W
Wen
Ji
J
Guan
Qun
Q
Zhang
Qiyao
Q
D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D001757:Blastomeres / Q000166:cytology* / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D015044:Zona Pellucida / Q000502:physiology*