Role of IL-5 during primary and secondary immune response to acetylcholine receptor.
To analyze the role of the Th2 cytokine interleukin-5 (IL-5) in experimental autoimmune myasthenia gravis (EAMG) pathogenesis, we induced clinical EAMG in C57BL/6 and IL-5 gene-knockout (KO) mice in the C57BL/6 background. IL-5 KO mice had a significantly reduced incidence and severity of EAMG. Despite their increased resistance to EAMG, IL-5 KO mice displayed intact secondary antibody and lymphoproliferative responses to acetylcholine receptor (AChR) after immunization with this molecule. However, the relative resistance of IL-5 KO mice was associated with a reduced primary lymphocyte response to AChR, and reduced C3 levels in muscle extracts compared to those in C57BL/6 mice.
Poussin
Mathilde A
MA
Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, 3.142 MRB, Galveston, TX 77555-1070, USA.
Goluszko
Elzbieta
E
Franco
Juan U
JU
Christadoss
Premkumar
P
D000276:Adjuvants, Immunologic / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001323:Autoantibodies / Q000097:blood; D003176:Complement C3 / Q000378:metabolism; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015848:Interleukin-5 / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000276:immunology / Q000378:metabolism; D009157:Myasthenia Gravis / Q000235:genetics* / Q000276:immunology*; D011379:Prognosis; D011950:Receptors, Cholinergic / Q000276:immunology*; D012720:Severity of Illness Index
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D003176:Complement C3; D000939:Epitopes; D015848:Interleukin-5; D011950:Receptors, Cholinergic; D016753:Interleukin-10; D007371:Interferon-gamma