Synthesis and NKT cell stimulating properties of fluorophore- and biotin-appended 6"-amino-6"-deoxy-galactosylceramides.
Alpha-galactosylceramides are potent stimulators of human T cells. Stimulation occurs through binding of the glycolipids by CD1d, presentation to T cells, and formation of a CD1d-glycolipid-T cell receptor complex. To facilitate the elucidation of the structural features of glycolipids necessary for T cell stimulation, alpha-galactosylceramides have been prepared with small molecules appended at the C6 position of the sugar. The appended molecules do not significantly influence the abilities of the glycolipids to stimulate T cells. [reaction: see text]
Zhou
Xiao-Ti
XT
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602, USA.
Forestier
Claire
C
Goff
Randal D
RD
Li
Chunhong
C
Teyton
Luc
L
Bendelac
Albert
A
Savage
Paul B
PB
D018949:Antigens, CD1 / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D005699:Galactosylceramides / Q000737:chemistry* / Q000494:pharmacology*; D006017:Glycolipids / Q000737:chemistry; D006801:Humans; D006825:Hybridomas; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007694:Killer Cells, Natural / Q000187:drug effects*; D013268:Stimulation, Chemical
D018949:Antigens, CD1; D002241:Carbohydrates; D005699:Galactosylceramides; D006017:Glycolipids; D007202:Indicators and Reagents; D001710:Biotin