Pharmacokinetics of paeoniflorin after oral administration of Shao-yao Gan-chao Tang in mice.
Paeoniflorin, a monoterpene glycoside, is the principal bioactive component of Paeoniae Radix. The traditional prescription Shao-yao Gan-chao Tang (SGT; Kampo: Shakuyaku-Kanzo-To), which is composed of Paeoniae Radix and Glycyrrhizae Radix, has been widely used in China and Japan. Quantification of paeoniflorin in mouse plasma after oral administration of SGT (at a dose containing 10 mg/kg paeoniflorin) was achieved using a simple and rapid high-performance liquid chromatography method. The plasma concentration-time curves were fitted with mean terminal half-lives (t 1/2) of 116.17 min. The maximum plasma concentration (Cmax) of paeoniflorin was 111.56 ng/ml, time to reach maximum concentration (tmax) was 17.00 min, the area under the plasma concentration-time curve (AUC)0-t was 12293.42 ng x min/ml, clearance/bioavailability (CL/F) value was 644.74 ml/min x kg, apparent volume of distribution/ bioavailability (Vd/F) value was 103.05 l/kg, and the mean residence time (MRT) was 169.64 min. These results, together with the previously reported kinetic data of paeoniflorin after oral administration of Paeoniae Radix extract alone, indicated that absorption of paeoniflorin after oral administration of SGT was significantly greater than that after oral administration of Paeoniae Radix alone.
Chen
Lih-Chi
LC
Department of Pharmacognosy, Graduate Institute of Pharmaceutical Sciences, Taipei Medical University, Taiwan.
Chou
Mei-Huei
MH
Lin
Ming-Fang
MF
Yang
Ling-Ling
LL
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005612:Freeze Drying; D005960:Glucosides / Q000493:pharmacokinetics*; D006207:Half-Life; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D039821:Monoterpenes; D010936:Plant Extracts / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D006027:Glycosides
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D006027:Glycosides