Over 1000 genes are involved in the DNA damage response of Escherichia coli.
Changes in gene expression after treatment of Escherichia coli cultures with mitomycin C were assessed using gene array technology. Unexpectedly, a large number of genes (nearly 30% of all genes) displayed significant changes in their expression level. Analysis and classification of expression profiles of the corresponding genes allowed us to assign this large number of genes into one or two dozen small clusters of genes with similar expression profiles. This assignment allowed us to describe systematically the changes in the level of gene expression in response to DNA damage. Among the damage-induced genes, more than 100 are novel. From those genes involved in DNA metabolism that have not previously been shown to be induced by DNA damage, the mutS gene involved in mismatch repair is especially noteworthy. In addition to the SOS response, we observed the induction of other stress response pathways, such as those of oxidative stress and osmotic protection. Among the genes that are downregulated in response to DNA damage are numerous protein biosynthesis genes. Analysis of the gene expression data highlighted the essential involvement of sigma(s)-regulated genes and the general stress response network in the response to DNA damage.
Khil
Pavel P
PP
Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892, USA.
Camerini-Otero
R Daniel
RD
D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D016000:Cluster Analysis; D004249:DNA Damage / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016685:Mitomycin / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis
D001426:Bacterial Proteins; D016685:Mitomycin