Environmental toxicology: polychlorinated biphenyls impair TNF-alpha release in vitro.
Tumour necrosis factor (TNF-alpha) was induced by bacterial lipopolysaccharides (LPS)/phytohemagglutin (PHA) stimulated human whole blood in vitro. Levels of TNF-alpha were evaluated by enzyme-linked immunosorbent assay. Blood samples treated with polychlorinated biphenyls (PCB 77, PCB 126) exhibited impairment of TNF-alpha release: 50 pg/microl PCB reduced by up to 66% and 500 pg/microl PCB reduced by up to 93% the TNF-alpha release compared with the controls.
Ahne
W
W
Faculty of Veterinary Medicine, Ludwig Maximilians University of Munich, München, Germany. ahne@lrz.uni-muenchen.de
Jarre
T
T
D001681:Biological Assay / Q000379:methods; D001773:Blood Cells / Q000187:drug effects* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D008070:Lipopolysaccharides; D010835:Phytohemagglutinins; D014409:Tumor Necrosis Factor-alpha; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls