Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide.
It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis ( beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a co-polymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
Volova
T G
TG
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 660036 Krasnoyarsk, Russia. volova@ibp.ru
Kalacheva
G S
GS
Altukhova
O V
OV
D002248:Carbon Monoxide / Q000378:metabolism*; D003470:Culture Media; D020118:Cupriavidus necator / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000737:chemistry; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D002248:Carbon Monoxide; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates