title: Modifications in astrocyte morphology and calcium signaling induced by a brain capillary endothelial cell line.
abstract: Astrocytes extend specialized endfoot processes to perisynaptic and perivascular regions, and thus are positioned to mediate the bidirectional flow of metabolic, ionic, and other transmissive substances between neurons and the blood stream. While mutual structural and functional interactions between neurons and astrocytes have been documented, less is known about the interactions between astrocytes and cerebrovascular cells. For example, although the ability of astrocytes to induce structural and functional changes in endothelial cells is established, the reciprocity of brain endothelial cells to induce changes in astrocytes is undetermined. This issue is addressed in the present study. Changes in primary cultures of neonatal mouse cortical astrocytes were investigated following their coculture with mouse brain capillary endothelial (bEnd3) cells. The presence of bEnd3 cells altered the morphology of astrocytes by transforming them from confluent monolayers into networks of elongated multicellular columns. These columns did not occur when either bEnd3 cells or astrocytes were cocultured with other cell types, suggesting that astrocytes undergo specific morphological consequences when placed in close proximity to brain endothelial cells. In addition to these structural changes, the pharmacological profile of astrocytes was modified by coculture with bEnd3 cells. Astrocytes in the cocultures showed an increased Ca2+ responsiveness to bradykinin and glutamate, but no change in responsiveness to ATP, as compared to controls. Coculturing the astrocytes with a neuronal cell line resulted in increased responsiveness of the glial responses to glutamate but not to bradykinin. These studies indicate that brain endothelial cells induce changes in astrocyte morphology and pharmacology.
authors.affiliation: Department of Neurology, UCLA School of Medicine, Los Angeles, California, USA. eyoder@ucsd.edu
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Yoder
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000502:physiology; D001921:Brain / Q000098:blood supply / Q000166:cytology; D020013:Calcium Signaling / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D051379:Mice