A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis.
SAG101, a leaf senescence-associated gene, was cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized. Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves. A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity. Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for approximately 4 days. Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants. These data suggest that SAG101 plays a significant role in leaf senescence.
He
Yuehui
Y
Plant Physiology/Biochemistry/Molecular Biology Program, Department of Agronomy, University of Kentucky, Lexington, KY 40546-0236, USA.
Gan
Susheng
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002734:Chlorophyll / Q000378:metabolism; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D030821:Plants, Genetically Modified; D016601:RNA-Binding Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000235:genetics; D013869:Thiolester Hydrolases / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases
D029681:Arabidopsis Proteins; D016373:DNA, Antisense; D018076:DNA, Complementary; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D002734:Chlorophyll; D003907:Dexamethasone; C118307:RNF7 protein, human; D044767:Ubiquitin-Protein Ligases; D002265:Carboxylic Ester Hydrolases; C460108:SAG101 protein, Arabidopsis; D013869:Thiolester Hydrolases; D005966:Glucuronidase