title: Purification and partial characterization of camel (Camelus Dromedarius) ceruloplasmin.
abstract: Adult and young camel ceruloplasmin (Cp) were isolated and purified using the single-step chromatography on amino ethyl-activated sepharose. There are no differences between the adult and the young camel protein. The molecular mass of the protein, as estimated by SDS-PAGE (denaturant conditions), was approximately 130000 Da. The electrophoretic mobility of camel Cp is slightly higher as compared to human and sheep protein suggesting that the camel Cp is homogeneous, compact and more acid. The copper content was estimated to be 5.8+/-0.3 atoms per molecule. The spectroscopic feature includes an absorption maximum at 610 nm, which could be attributed to type 1 copper. The EPR spectrum was completely devoid of any typical signal of the type 2 copper. The kinetic parameters of the adult camel Cp for the specific activity as p-phenylendiamine oxidase were determined as K(m)=0.42 mM and V(max)=0.93 microM NADH/mn/mg Cp. The optimum pH for the activity was 5.7.
authors.affiliation: Laboratoire de Biochimie, Université Hassan 1er, Faculté des Sciences et Techniques de Settat, BP 577, 26000, Settat, Morocco.
authors.forename: Abdel Khalid
authors.initials: AK
authors.lastname: Essamadi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bengoumi
authors.forename: Driss
authors.initials: D
authors.lastname: Zaoui
authors.forename: Bernard
authors.initials: B
authors.lastname: Faye
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Bellenchi
authors.forename: Giovani
authors.initials: G
authors.lastname: Musci
authors.forename: Lilia
authors.initials: L
authors.lastname: Calabrese
mesh_terms: D000818:Animals; D002162:Camelus; D002570:Ceruloplasmin / Q000302:isolation & purification* / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D013053:Spectrophotometry
chemical_list: D003300:Copper; D002570:Ceruloplasmin; C022345:4-phenylenediamine oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors