Effect of an amine-fluoride-triclosan mouthrinse on plaque regrowth and biofilm vitality.
BACKGROUND
The purpose of this double-blind, prospective, latin-square crossover randomised study was to examine the efficacy of a mouthrinse solution containing a combination of triclosan, amine fluoride and sodium fluoride on supragingival plaque regrowth compared to a placebo and a chlorhexidine solution.


METHODS
12 volunteers refrained after professional oral prophylaxis from all mechanical hygiene measures for the following 96 h and rinsed instead cross-over in a randomised order with either chlorhexidine digluconate (0.2%, positive control), a verum solution (0.5% amine fluoride, 0.028% sodium fluoride, 0.03% triclosan) or a placebo solution. The plaque index was assessed after 24 and 96 h (PI 1, PI 2) and the plaque area (PA) photographed and calculated after 96 h of undisturbed plaque regrowth. Moreover plaque samples were taken after 24 and 96 h and examined with the vital fluorescence technique to assess the vitality of the biofilm microbiota (VF 1, VF 2).


RESULTS
The amine-fluoride-triclosan solution reduced the clinical parameters (PI and PA) as well as the vitality of the plaque flora (VF) significantly when compared to the placebo solution. The verum reached a 36.5% (p<0.05) and a 39.8% reduction (p<0.0001) in PI 1 and PI 2, respectively, concomittant with a reduction of 23.8% and 32.2% (p<0.001) in VF 1 and VF 2 and of 46.9% (p<0.0001) in PA at day 4. This was less than the reductions found with the positive control, i.e., the 0.2% chlorhexidine solution (54.2% and 71.1% reduction in PI 1 and PI 2, 40.0% and 53.4% in VF 1 and VF 2 and 71.5% in PA). However, significant differences between both active solutions were only established for PI 2 and PA.


CONCLUSIONS
During 4-day plaque regrowth the amine-fluoride-triclosan product displayed a significant antibacterial and plaque-reducing action in comparison to the control.
Arweiler
Nicole B
NB
Albert-Ludwigs-University of Freiburg, Dept. of Operative Dentistry and Periodontology, Freiburg, Germany. arweiler@zmk2.ukl.uni-freiburg.de
Henning
G
G
Reich
E
E
Netuschil
L
L
D000293:Adolescent; D000328:Adult; D000588:Amines / Q000008:administration & dosage / Q000627:therapeutic use*; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D018592:Cross-Over Studies; D003773:Dental Plaque / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D003774:Dental Plaque Index; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009067:Mouthwashes / Q000627:therapeutic use*; D010512:Periodontal Index; D010919:Placebos; D011446:Prospective Studies; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000627:therapeutic use; D013223:Statistics as Topic; D018709:Statistics, Nonparametric; D014260:Triclosan / Q000008:administration & dosage / Q000627:therapeutic use*
D000588:Amines; D000891:Anti-Infective Agents, Local; D009067:Mouthwashes; D010919:Placebos; D014260:Triclosan; D012969:Sodium Fluoride; C010882:chlorhexidine gluconate; D005459:Fluorides; D002710:Chlorhexidine