PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains.
OBJECTIVE
The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains).


RESULTS
On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With a combination of these three enzymes, 22 types were found. In human strains, the most frequently occurring types were Cj.4 (28%), Cj.1 (19%), Cj. 13 (13%) and Cj. 2 (5%). In the case of poultry strains, the most frequent types were Cj. 1 (34%), Cj. 11 (22%), C.j. 21 (16%) and Cj. 15 (11%).


CONCLUSIONS
The findings support the hypothesis that poultry is a significant source but not sole source of Campylobacter sp. in relation to humans.


CONCLUSIONS
The typing of Campylobacter sp. forms the basis for an evaluation of the current state and risk assessment of various Campylobacter sp. sources in relation to humans.
Steinhauserova
I
I
University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic. steinhauserovai@vfu.cz
Ceskova
J
J
Nebola
M
M
D000818:Animals; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry / Q000382:microbiology*
D005408:Flagellin