title: Hepatic microsomal cytochrome p450s and chlorinated hydrocarbons in largha and ribbon seals from Hokkaido, Japan: differential response of seal species to Ah receptor agonist exposure.
abstract: From 16 largha seals (Phoca largha) and 15 ribbon seals (Phoca fasciata) in the coastal waters of Hokkaido, Japan, blubber chlorinated hydrocarbon (CHC) levels and hepatic cytochrome P450 (CYP) catalytic activities and their immunochemically detected protein content levels were measured. Concentrations of DDTs (2,2-bis(4-chlorophenyl)-1,1-dichloroethylene,p,p'-DDE; 2,2-bis(4-chlorophenyl)-1,1-dichloroethane, p,p'-DDD; dichlorodiphenyltrichloroethane, p,p'-DDT), polychlorinated biphenyl congeners (PCBs), and chlordane compounds (oxychlordane, chlordanes, and nonachlors) in both species were in the range of 290 to 5,300, 420 to 4,000, and 130 to 1,500 ng/g lipid weight, respectively. Aryl hydrocarbon receptor (AhR) agonists, non-ortho (IUPAC 77 and 126) and mono-ortho (IUPAC 105, 118, and 156) coplanar PCB congeners, were also detected, and the 2,3,7,8-tetrachlordibenzo-p-dioxin (TCDD) toxic equivalents (TEQs) were 4.9 to 120 pg TEQ/g lipid weight. Cross-reactive proteins with polyclonal antibodies against rat CYP1A1 and CYP3A2 were notably detected in seal liver microsomes. Interestingly, a polyclonal antibody against rat CYP2B1 recognized proteins only at trace levels. In largha seals, both levels of alkoxyresorufin- (methoxy-, ethoxy-, pentoxy-, and benzyloxyresorufin) O-dealkylase (AROD) activities and proteins detected by polyclonal antibodies against rat CYP1A1 were significantly correlated with the concentrations of individual coplanar PCB congeners, total TEQs, and total PCBs. Threshold concentrations for TEQs in blubber of the largha seal to induce hepatic CYP1A protein and EROD activity were estimated to be 8.5 and 19 pg TEQ/g fat weight, respectively. In ribbon seals, similar correlations were not detected, although the TEQ levels were not significantly lower than those in largha seals. These results suggest that AROD activity and CYP1A1 protein in the liver of the largha seal could be a biomarker for the exposure to AhR agonists such as coplanar PCB congeners. This study also indicates differences in AhR-mediated responses to the CHC exposures between largha and ribbon seals.
authors.affiliation: Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Issei
authors.initials: I
authors.lastname: Chiba
authors.forename: Akihito
authors.initials: A
authors.lastname: Sakakibara
authors.forename: T Hisato
authors.initials: TH
authors.lastname: Iwata
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Tanabe
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Akahori
authors.forename: Akio
authors.initials: A
authors.lastname: Kazusaka
authors.forename: Shoichi
authors.initials: S
authors.lastname: Fujita
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000494:pharmacology*; D004781:Environmental Exposure; D004790:Enzyme Induction; D005260:Female; D007306:Insecticides / Q000009:adverse effects*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D011078:Polychlorinated Biphenyls / Q000009:adverse effects*; D046048:Seals, Earless; D014874:Water Pollutants, Chemical / Q000009:adverse effects*
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls