Visualisation of the actin cytoskeleton by cryo-electron microscopy.
An understanding of the mechanisms driving cell motility requires clarification of the structural organisation of actin filament arrays in the regions of cell protrusion termed lamellipodia. Currently, there is a lack of consensus on lamellipodia organisation stemming from the application of alternative procedures for ultrastructural visualisation of cytoskeleton networks. In this study, we show that cryo-electron microscopy of extracted cytoskeletons embedded in a thin layer of vitreous ice can reveal the organisation of cytoskeletal elements at high resolution. Since this method involves no dehydration, drying and contrasting steps that can potentially introduce subtle distortions of filament order and interactions, its application opens the way to resolving the controversial details of lamellipodia architecture.
Resch
Guenter P
GP
Institute of Molecular Biology, Department Cell Biology, Billrothstrasse 11, A-5020 Salzburg, Austria.
Goldie
Kenneth N
KN
Krebs
Angelika
A
Hoenger
Andreas
A
Small
J Victor
JV
D008841:Actin Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D005057:Eukaryotic Cells / Q000378:metabolism / Q000648:ultrastructure*; D008567:Membranes, Artificial; D051379:Mice; D011554:Pseudopodia / Q000378:metabolism / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured
D008567:Membranes, Artificial