Calretinin and FMRFamide immunoreactivity in the nervus terminalis of prenatal tree shrews (Tupaia belangeri).
The distribution and development of FMRFamide- and calretinin-immunoreactive neurons were investigated in the nervus terminalis of prenatal tree shrews from gestation day 19 onwards. The first FMRFamide-immunoreactive cells were observed medially in the olfactory epithelium on gestation day 20. From gestation day 23 onwards, the migrating nervus terminalis ganglion cells showed FMRFamide calretinin immunoreactivity. The distribution pattern of FMRFamide- and calretinin-immunoreactive cells was similar along the migratory route and in the ganglion of the terminal nerve. However, most probably calretinin and FMRFamide were expressed in separate neuronal populations. For the first time in a mammal, FMRFamide and calretinin are reported to occur in the migrating perikarya and neuronal processes of the nervus terminalis during prenatal development. The results suggest (i) an early activation of the rostral FMRFamide-immunoreactive migratory stream comparable to that described for the GnRH-immunoreactive part of the terminal nerve in other mammals and possibly (ii) an involvement of calretinin in mechanisms of cell migration and outgrowth of neuronal processes in the terminal nerve during the studied period.
Malz
Cordula Renate
CR
Department of Morphology, Center of Anatomy, School of Medicine, University of Göttingen, Kreuzbergring 36, 37075 Göttingen, Germany. cmalz@gwdg.de
Kuhn
Hans-Jürg
HJ
D000818:Animals; D064032:Calbindin 2; D002465:Cell Movement; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005314:Embryonic and Fetal Development; D019835:FMRFamide / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000502:physiology; D009830:Olfactory Bulb / Q000196:embryology*; D009833:Olfactory Pathways / Q000196:embryology*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D012788:Shrews / Q000196:embryology*
D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D019835:FMRFamide