[Relationship between proliferation of vascular smooth muscle cells and PDGF-AA and PDGFR-alpha expression in SHRs].
To explore the role of platelet derived growth factor-AA (PDGF-AA) and PDGFR-alpha expression in the proliferation and hypertrophy of vascular smooth muscle cells (VSMCs) in spontaneously hypertension rats (SHR), protein expression of PDGF-AA, PDGFR-alpha and PDGFR-beta in SHR/Wistar-Kyoto (WKY)-VSMC was observed by Western blot. Proliferation and hypertrophy of SHR-VSMCs induced by PDGF-AA were observed by measurement of PCNA and [(3)H] incorporation. PDGF-AA and PDGFR-alpha expression was markedly increased in SHR-VSMCs compared with that in WKY-VSMCs, but PDGFR-beta was not different in SHR and WKY-VSMCs. PDGF-AA induced PCNA expression and [(3)H] incorporation was increased in a dose-dependent manner in SHR, but not in WKY. It is suggested that an enhancement of PDGF-AA and PDGFR-alpha in SHRs may be one of the important factors for vascular modeling.
Jin
Jun
J
Department of Cardiology, Xingqiao Hospital, Third Military Medical University, Chongqing 400037.
Zhu
Shan-Jun
SJ
Zhu
Zhi-Ming
ZM
Yang
Yong-Jian
YJ
Ding
Gang
G
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006973:Hypertension / Q000503:physiopathology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000096:biosynthesis*
D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A; D020796:Receptor, Platelet-Derived Growth Factor alpha