title: Directed evolution of enzymes and pathways for industrial biocatalysis.
abstract: Directed evolution has become a powerful tool for developing enzyme and whole cell based biocatalysts. Significant recent advances include the creation of novel enzyme functions and the development of several new efficient directed evolution methods. The combination of directed evolution and rational design promises to accelerate the development of biocatalysts for applications in the pharmaceutical, chemical and food industries.
authors.affiliation: Department of Chemical Engineering, Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA. zhao5@uiuc.edu
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
authors.forename: Karuppiah
authors.initials: K
authors.lastname: Chockalingam
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002384:Catalysis; D019020:Directed Molecular Evolution / Q000379:methods* / Q000639:trends*; D004795:Enzyme Stability; D004798:Enzymes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D012680:Sensitivity and Specificity
chemical_list: D004798:Enzymes