title: [Cloning and expression of Buthus martensii Karsch scorpion toxin gene (BmK IT3) in Escherichia coli].
abstract: According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.
authors.affiliation: College of Life Sciences, Suzhou University, Suzhou 210006, China.
authors.forename: Ji-Bin
authors.initials: JB
authors.lastname: Yu
authors.forename: Ping
authors.initials: P
authors.lastname: Ji
authors.forename: Xin-Min
authors.initials: XM
authors.lastname: Zha
authors.forename: Wei-De
authors.initials: WD
authors.lastname: Shen
authors.forename: Xiang-Fu
authors.initials: XF
authors.lastname: Wu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012604:Scorpion Venoms / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D012605:Scorpions / Q000737:chemistry / Q000235:genetics*
chemical_list: C457223:BmK IT(3) scorpion toxin; D011994:Recombinant Proteins; D012604:Scorpion Venoms; D007544:Isopropyl Thiogalactoside