title: Efficacy of common laboratory disinfectants on the infectivity of Cryptosporidium parvum oocysts in cell culture.
abstract: Nine liquid disinfectants were tested for their ability to reduce infectivity of Cryptosporidium parvum oocysts in cell culture. A 4-min exposure to 6% hydrogen peroxide and a 13-min exposure to ammonium hydroxide-amended windshield washer fluid reduced infectivity 1,000-fold. Other disinfectants tested (70% ethanol, 37% methanol, 6% sodium hypochlorite, 70% isopropanol, and three commercial disinfectants) did not reduce the infectivity after a 33-min exposure. The results indicate that hydrogen peroxide and windshield washer fluid or ammonium hydroxide disinfectant may be suitable laboratory disinfectants against C. parvum oocysts.
authors.affiliation: Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: Susan C
authors.initials: SC
authors.lastname: Weir
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Pokorny
authors.forename: Ramon A
authors.initials: RA
authors.lastname: Carreno
authors.forename: Jack T
authors.initials: JT
authors.lastname: Trevors
authors.forename: Hung
authors.initials: H
authors.lastname: Lee
mesh_terms: D064753:Ammonium Hydroxide; D000818:Animals; D018929:Cell Culture Techniques; D003049:Coccidiostats / Q000494:pharmacology; D016785:Cryptosporidium parvum / Q000187:drug effects*; D004202:Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006878:Hydroxides / Q000494:pharmacology; D007466:Iodophors / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D003049:Coccidiostats; D004202:Disinfectants; D006878:Hydroxides; D007466:Iodophors; D000431:Ethanol; D011092:Polyethylene Glycols; D064753:Ammonium Hydroxide; C015573:Wescodyne; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D000432:Methanol