Interleukin-1 beta and interferon-gamma induce proliferation and apoptosis in cultured Schwann cells.
This study reports that in Schwann cell tissue culture the administration of the two pro-inflammatory cytokines, interleukin-1 beta (IL-1 beta) and interferon-gamma (IFN-gamma), at different dosages, singly or in combination, can induce apoptosis and/or mitosis. Schwann cell apoptosis was maximal within 24 h of stimulation with 50 U/ml of IFN-gamma, while proliferation was at its peak within 24 h with 10 U/ml IL-1 beta, and both processes decreased progressively by 48 and 72 h. Moreover, the combination of the two cytokines did not show any synergistic effect. These data can be interpreted as a possible involvement of pro-inflammatory cytokines not only in myelin disruption but also in promoting remyelination.
Conti
Giancarlo
G
Department of Neurological Sciences, Dino Ferrari Center, University of Milan, IRCCS Ospedale Maggiore Policlinico, Via F. Sforza 35, 20122 Milan, Italy.
De Pol
Anto
A
Scarpini
Elio
E
Vaccina
Fabiana
F
De Riz
Milena
M
Baron
PierLuigi
P
Tiriticco
Marco
M
Scarlato
Guglielmo
G
D000818:Animals; D017209:Apoptosis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D018836:Inflammation Mediators / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
D004338:Drug Combinations; D018836:Inflammation Mediators; D007375:Interleukin-1; D007371:Interferon-gamma