Identification of TH1 as an interaction partner of A-Raf kinase.
A-Raf is an important intermediate of the growth factor Ras-MAP kinase pathway. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf. TH1 is a highly conserved and widely expressed protein, which was recently cloned by Bonthron DT group. The binding between A-Raf and TH1 was specific, as no binding between TH1 and B-Raf or c-Raf was observed, and the amino-terminal 162 amino acids in the A-Raf regulatory domain were found to be sufficient for this interaction. This specific interaction may have played a critical role in the activation of A-Raf.
Yin
Xiang L
XL
Gene Research Center, Medical Center of Fudan University, Shanghai, People's Republic of China.
Chen
She
S
Gu
Jian X
JX
D015153:Blotting, Western; D002460:Cell Line; D006801:Humans; D008099:Liver / Q000378:metabolism; D010766:Phosphorylation; D011485:Protein Binding; D048491:Proto-Oncogene Proteins A-raf; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D020798:Two-Hybrid System Techniques
D048491:Proto-Oncogene Proteins A-raf; D019908:Proto-Oncogene Proteins c-raf