title: Induction of protective immunity to Listeria monocytogenes by immunization with plasmid DNA expressing a helper T-cell epitope that replaces the class II-associated invariant chain peptide of the invariant chain.
abstract: Listeria epitope-specific helper T (Th) cells were able to be primed and induced in vivo by immunization with a plasmid carrying an invariant chain (Ii) gene whose class II-associated invariant chain peptide (CLIP) region was replaced by a Listeria Th epitope. Immunization of C3H/He mice with an Ii-LLO 215-226 plasmid induced specific interferon-gamma- and interleukin 2-producing Th cells and conferred significant protective immunity against listerial infection.
authors.affiliation: Department of Microbiology and Immunology, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, Japan. tnagata@hama-med.ac.jp
authors.forename: Toshi
authors.initials: T
authors.lastname: Nagata
authors.forename: Taiki
authors.initials: T
authors.lastname: Aoshi
authors.forename: Mina
authors.initials: M
authors.lastname: Suzuki
authors.forename: Masato
authors.initials: M
authors.lastname: Uchijima
authors.forename: Yeung-Hyen
authors.initials: YH
authors.lastname: Kim
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Yang
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koide
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics* / Q000276:immunology; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000276:immunology; D006460:Hemolysin Proteins; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D010446:Peptide Fragments / Q000276:immunology; D010957:Plasmids; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D018984:Epitopes, T-Lymphocyte; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D010446:Peptide Fragments; D019444:Vaccines, DNA; C045084:invariant chain; D007371:Interferon-gamma; C038705:hlyA protein, Listeria monocytogenes