Peritoneal closure reduces port site metastases: results of an experiment in a rat ovarian cancer model.
BACKGROUND
The arm of this study was to assess the role of peritoneal closure in the prevention of port site metastasis after carbon dioxide (CO2) CO2 pneumoperitoneum.


METHODS
We developed a xenograft ovarian cancer model by intraperitoneal injection of 27 106 IGR-OV1 line cells in nude rats Seven days after the inoculation, the animals underwent a CO2 pneumoperitoneum. At the end of the procedure, port sites were randomly closed either with suture of peritoneum (n = 14, group A) or without suture of peritoneum (n = 12, group B). The rats were killed 7 days after surgery and their port site scars were resected. Tumor implantation was assessed by a pathologist who was blinded to the type of wound closure.


RESULTS
The animals in group B were significantly more likely to have at least one port site metastasis frequent (seven of 12, or 58.3%) than those in group A (two of 14, or (14.3%) (p = 0.037). Port sites with metastases were seen more frequently in group B (eight of 24, or (33.3%) than in group A (three of 28, or 10.7%) (p = 0.046).


CONCLUSIONS
Our results shows that peritoneum closure decreases the risk of port site metastasis.
Agostini
A
A
Department of Obstetrics and Gynecology, La Conception University Hospital, 147 Boulevard Baille, 13005, Marseille, France. aubert.agostini@wanadoo.fr
Robin
F
F
Jais
J P
JP
Aggerbeck
M
M
Vildé
F
F
Blanc
B
B
Lécuru
F
F
D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D007327:Insufflation / Q000379:methods; D010535:Laparoscopy / Q000379:methods; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation / Q000379:methods; D010051:Ovarian Neoplasms / Q000556:secondary* / Q000601:surgery; D010537:Peritoneum / Q000601:surgery*; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous / Q000379:methods; D014407:Tumor Cells, Cultured
D002245:Carbon Dioxide