Electrophysiological characterization of retinal Müller glial cells from mouse during postnatal development: comparison with rabbit cells.
The electrophysiology of murine Müller cells, and of their precursors, during postnatal development was investigated by using the whole-cell patch-clamp technique. Membrane potential, membrane capacitance, and expression of voltage-gated Na+ currents increased during the first 3 postnatal weeks. During the same period, the membrane resistance decreased due to an upregulation of both inward and outward K+ currents. Glutamate transporter-mediated currents increased during the first postnatal weeks, as well. In Müller (precursor) cells from rabbits, these transporter currents already achieved adult levels at postnatal day 6, the earliest stage studied. Together with the developmental time course of K+ currents, this indicates a delay in the maturation of murine Müller cells, as compared with cells from rabbit.
Pannicke
Thomas
T
Paul-Flechsig-Institut für Hirnforschung, Universität Leipzig, Germany. pant@medizin.uni-leipzig.de
Bringmann
Andreas
A
Reichenbach
Andreas
A
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001464:Barium / Q000494:pharmacology; D002454:Cell Differentiation / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D027342:Excitatory Amino Acid Transporter 2 / Q000187:drug effects / Q000378:metabolism; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D016194:Receptors, N-Methyl-D-Aspartate / Q000819:agonists / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000254:growth & development* / Q000502:physiology*; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology
D027342:Excitatory Amino Acid Transporter 2; D007473:Ion Channels; D015221:Potassium Channels; D016194:Receptors, N-Methyl-D-Aspartate; D015222:Sodium Channels; D001464:Barium; D018698:Glutamic Acid; D016202:N-Methylaspartate