title: Blood flow distribution within the airway wall.
abstract: Altered perfusion of the bronchial mucosal plexus relative to the adventitial plexus may contribute to geometric changes in the airway wall and lumen. We studied bronchial perfusion distribution in sheep by using fluorescent microspheres at baseline and during intrabronchial artery challenge with methacholine chloride (MCh; n = 7). Additionally, we measured airway resistance (Raw) during MCh with control or increased perfusion (n = 9). Raw with MCh was significantly greater for high than control flow. Microspheres in histological sections lodged predominantly in the mucosa (60%), and this was not altered by MCh. However, more microspheres lodged in airways >1-mm in diameter during MCh and increased perfusion than MCh and control flow. In airways < or =1 mm in diameter, fewer microspheres lodged during control than increased flow. If the number of microspheres represents regional agonist access to airway smooth muscle, then the differences observed in Raw can be explained by the distribution of agonist. During challenge, there was greater MCh delivery to larger airways during increased flow and less delivery to smaller airways during control flow. The results demonstrate the effects of axial perfusion distribution on Raw.
authors.affiliation: Department of Medicine, Johns Hopkins University, Baltimore, Maryland 21224, USA. wagnerem@jhmi.edu
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Wagner
authors.forename: Robert H
authors.initials: RH
authors.lastname: Brown
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D001980:Bronchi / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology; D001981:Bronchial Arteries / Q000502:physiology; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology; D016085:Bronchoconstrictor Agents / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D007269:Injections, Intra-Arterial; D016210:Methacholine Chloride / Q000008:administration & dosage; D008863:Microspheres; D023421:Models, Animal; D009130:Muscle, Smooth / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology; D011652:Pulmonary Circulation / Q000187:drug effects / Q000502:physiology*; D020545:Respiratory Mucosa / Q000098:blood supply* / Q000502:physiology; D012756:Sheep; D014665:Vasodilator Agents / Q000008:administration & dosage
chemical_list: D016085:Bronchoconstrictor Agents; D005456:Fluorescent Dyes; D014665:Vasodilator Agents; D016210:Methacholine Chloride