Human myosin XVBP is a transcribed pseudogene.
A novel human myosin gene located at 17q25 was identified through evaluation of genomic DNA sequence and designated myosin XVBP since it resembled human myosin XVA. In humans, myosin XVBP along with an adjacent gene, Lethal Giant Larvae 2 (LLGL2) appears to have arisen from a genomic duplication of a chromosomal interval that included LLGL and an ancestral myosin XV. Inspection of human myosin XVBP predicted amino acid sequence from genomic DNA revealed that 36 of the 131 conserved amino acid residues of the motor domain are substituted or deleted, including sequence changes within the regions involved in the binding of ATP and actin. Twelve myosin XVBP overlapping cDNAs from kidney and stomach mRNA samples were cloned and sequenced. Analyses of these myosin XVBP cDNAs revealed numerous additional disablements including translational reading frame shifts resulting in stop codons. From these data we conclude that myosin XVBP is a transcribed, unprocessed pseudogene.
Boger
E T
ET
Laboratory of Molecular Genetics, Section on Human Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, Maryland 20850, USA.
Sellers
J R
JR
Friedman
T B
TB
D000199:Actins / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002886:Chromosomes, Human, Pair 17; D018388:Codon, Terminator; D017124:Conserved Sequence; D016368:Frameshift Mutation; D020440:Gene Duplication; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009218:Myosins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D000199:Actins; D018388:Codon, Terminator; C456583:MYO15B protein, human; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D018995:Myosin Heavy Chains; D009218:Myosins