title: [Rapid determination of propofol in biological specimens and hair by HS-GC/MS].
abstract: This paper proposes an original method for the determination of propofol in biological specimens. For example, hair specimens are cut into small pieces and incubated overnight in Soerensen buffer at 40 degrees C. After headspace preparation of the biological specimens spiked with tetrahydrofurane (internal standard), the analytes are transferred to the gas chromatograph and separated on a HP Wax capillary column. Detection is achieved in SIM mode (propofol: m/z 117, 163 and 178) on a mass spectrometer operating in electronic impact mode. The developed procedure is easy (full-automated), rapid (no extraction- or derivatization-step), sensible and accurate. After validation of the analytical method (linearity, extraction recovery, repeatability, limit of detection), this new procedure was applied to forensic cases.
authors.affiliation: Institut de Médecine Légale Laboratoire de toxicologie 11, rue Humann, 67085 Strasbourg, France. vincent.cirimele@medecine.u-strasbg.fr
authors.forename: V
authors.initials: V
authors.lastname: Cirimele
authors.forename: P
authors.initials: P
authors.lastname: Kintz
authors.forename: B
authors.initials: B
authors.lastname: Ludes
mesh_terms: D018686:Anesthetics, Intravenous / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry; D006801:Humans; D015742:Propofol / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D018686:Anesthetics, Intravenous; D015742:Propofol