title: Reduced levels of gamma-interferon secretion in response to chlamydial 60 kDa heat shock protein amongst women with pelvic inflammatory disease and a history of repeated Chlamydia trachomatis infections.
abstract: Peripheral lymphocytes in uninfected fertile controls, women with various histories of Chlamydia trachomatis infection, pelvic inflammatory disease (PID) and infertility not due to C. trachomatis infection (endometriosis) were cultured overnight with PHA mitogen and the 60 kDa chlamydial heat shock protein. Plasma samples were then assayed for levels of gamma-interferon and IL-10 using a commercial ELISA system. Women with PID and those with a history of multiple C. trachomatis infections showed reduced gamma-interferon production in response to cHSP60, not seen in women infected only once and those with infertility due to other causes (endometriosis). Secretion of IL-10 in response to cHSP60 did not vary significantly across the various patient groups, though all patients showed elevated levels of total IL-10 compared with uninfected controls.
authors.affiliation: Centre for Molecular Biotechnology, Queensland University of Technology, 484 Adelaide Street, Gardens Point Campus, Brisbane, Qld 4000, Australia. joedebat@powerup.com.ua
authors.forename: J
authors.initials: J
authors.lastname: Debattista
authors.forename: P
authors.initials: P
authors.lastname: Timms
authors.forename: J
authors.initials: J
authors.lastname: Allan
authors.forename: J
authors.initials: J
authors.lastname: Allan
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000494:pharmacology*; D002690:Chlamydia Infections / Q000276:immunology*; D002692:Chlamydia trachomatis; D005260:Female; D006801:Humans; D007246:Infertility / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D000292:Pelvic Inflammatory Disease / Q000276:immunology*; D012008:Recurrence; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; D016753:Interleukin-10; C067562:protein 60K, Chlamydia trachomatis; D007371:Interferon-gamma