5 polymorphisms in the transforming growth factor-beta 1 gene (TGF-beta 1) in adult periodontitis.
OBJECTIVE
Transforming growth factor-beta (TGF-beta) represents a family of polypeptide growth factors, involved in inflammation and regulation of immune responses. The purpose of this study was to determine whether polymorphisms in the TGF-beta 1 gene may confer susceptibility to adult periodontitis.


METHODS
We studied 90 patients with adult periodontitis together with 108 unrelated subjects. 3 polymorphisms located in the 5'region at positions -988 (C/A), -800 (G/A) and -509 (C/T) and 2 polymorphisms located at codons 10 (L10P) and 25 (R25P) of exon 1 were investigated by PCR methods.


RESULTS
There was no statistically-significant difference in genotype or allele frequency distributions between patients and reference group for the -800G/A, -509C/T, L10P and R25P polymorphisms (p>0.05 in all cases). The -988 A polymorphism was present neither in our patients nor in unrelated subjects. Upon stratification for smoking status no significant differences were found in the TGF-beta 1 genotype or allele frequencies either between adult periodontitis smokers compared to control smokers, or between periodontitis non-smokers and control non-smokers.


CONCLUSIONS
These data indicate that the mentioned polymorphisms of the TGF-beta 1 gene do not influence susceptibility to adult periodontitis. There was no association between any polymorphisms in the TGF-beta 1 gene, severity of periodontitis and the smoking status in our study.
Holla
Lydie Izakovicova
LI
Institute of Pathological Physiology, Medical Faculty, Masaryk University Brno, Czech Republic. holla@med.muni.cz
Fassmann
Antonin
A
Benes
Petr
P
Halabala
Tomas
T
Znojil
Vladimir
V
D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003062:Codon / Q000235:genetics; D016001:Confidence Intervals; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D009010:Monte Carlo Method; D016017:Odds Ratio; D010518:Periodontitis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012907:Smoking; D013223:Statistics as Topic; D016212:Transforming Growth Factor beta / Q000235:genetics*; D053773:Transforming Growth Factor beta1
D003062:Codon; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1