title: Aspergillus nidulans sod(VI)C1 mutation causes defects in cell wall biogenesis and protein secretion.
abstract: Growth at the restrictive temperature (42 degrees C) of Aspergillus nidulans B120, carrying the conditional-lethal mutation sod(VI)C1, was partially improved by the addition of 1.0 M sorbitol to the medium. The mutant grown at 42 degrees C, with osmotic stabilizer, showed abnormal hyphal morphology, a decrease in beta-1,3-glucan synthase activity as well as cell wall sugar content, but an increase in chitin synthase activity and N-acetyl-glucosamine content. The mutation also affected the secretion of extracellular protease. The temperature-dependent osmo-sensitive phenotype of a Saccharomyces cerevisiae alpha-COP mutation can be rescued by the A. nidulans sod(VI)C(+) gene. These results indicate that the sod(VI)C1 mutation affects proper processing of secretory proteins destined for the surface of cells or beyond.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Hwan Hee
authors.initials: HH
authors.lastname: Lee
authors.forename: Jeong Seok
authors.initials: JS
authors.lastname: Park
authors.forename: Suhn Kee
authors.initials: SK
authors.lastname: Chae
authors.forename: Pil Jae
authors.initials: PJ
authors.lastname: Maeng
authors.forename: Hee Moon
authors.initials: HM
authors.lastname: Park
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002687:Chitin Synthase / Q000378:metabolism; D020756:Coatomer Protein / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000502:physiology*; D005800:Genes, Fungal; D005936:Glucans / Q000096:biosynthesis; D009154:Mutation; D009995:Osmosis; D013012:Sorbitol / Q000494:pharmacology; D013696:Temperature; D047071:beta-Glucans
chemical_list: D020756:Coatomer Protein; D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; D013012:Sorbitol; C033363:beta-1,3-glucan; D002687:Chitin Synthase