title: A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.
abstract: A member of the mitogen-activated protein kinase superfamily, MAK, has been proposed to have an important role in spermatogenesis, since Mak gene expression is highly restricted to testicular germ cells. To assess the biological function of MAK, we have established MAK-deficient (Mak(-/-)) mice. Mak(-/-) mice developed normally, and no gross abnormalities were observed. Spermatogenesis of the Mak(-/-) mice was also intact, and most of the mice were fertile. However, Mak(-/-) male-derived litter sizes and their sperm motility in vitro were mildly reduced. These data show that function of MAK is not essential for spermatogenesis and male fertility.
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan. yshinkai@virus.kyoto-u.ac.jp
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shinkai
authors.forename: Hideo
authors.initials: H
authors.lastname: Satoh
authors.forename: Naoki
authors.initials: N
authors.lastname: Takeda
authors.forename: Mikiko
authors.initials: M
authors.lastname: Fukuda
authors.forename: Emiko
authors.initials: E
authors.lastname: Chiba
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kato
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuramochi
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005298:Fertility; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000201:enzymology*
chemical_list: D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081931:MAK protein, human; C509962:Mak protein, mouse