Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducensMBEL55E, from bovine rumen.
A novel succinic acid-producing bacterium was isolated from bovine rumen. The bacterium is a non-motile, non-spore-forming, mesophilic and capnophilic gram-negative rod or coccobacillus. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the recently reclassified genus Mannheimia as a novel species, and has been named Mannheimia succiniciproducens MBEL55E. Under 100% CO(2) conditions, it grows well in the pH range of 6.0-7.5 and produces succinic acid, acetic acid and formic acid at a constant ratio of 2:1:1. When M. succiniciproducensMBEL55E was cultured anaerobically in medium containing 20 g l(-1) glucose as carbon source, 13.5 g l(-1) of succinic acid was produced.
Lee
P C
PC
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea.
Lee
S Y
SY
Hong
S H
SH
Chang
H N
HN
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002417:Cattle / Q000382:microbiology*; D005285:Fermentation; D005561:Formates / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D019344:Lactic Acid / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012417:Rumen / Q000382:microbiology*; D019802:Succinic Acid / Q000378:metabolism*
D005561:Formates; D012336:RNA, Ribosomal, 16S; C030544:formic acid; D002245:Carbon Dioxide; D019344:Lactic Acid; D019802:Succinic Acid; D019342:Acetic Acid