Proliferation and telomere length in acutely mobilized blood mononuclear cells in HIV infected patients.
The aim of the study was to investigate the mobilization of T cells in response to a stressful challenge (adrenalin stimulation), and to access T cells resided in the peripheral lymphoid organs in HIV infected patients. Seventeen patients and eight HIV seronegative controls received an adrenalin infusion for 1 h. Blood was sampled before, during and 1 h after adrenalin infusion. Proliferation and mean telomere restriction fragment length (telomeres) of blood mononuclear cells (BMNC) and purified CD8+ and CD4+ cells were investigated at all time points. In patients, the proliferation to pokeweed mitogens (PWM) was lower and decreased more during adrenalin infusion. After adrenalin infusion the proliferation to PWM was restored only in the controls. In all subjects telomeres in CD4+ cells declined during adrenalin infusion. Additionally, the patients had shortened telomeres in their CD8+ cells, and particularly HAART treated patients had shortened telomeres in all cell-subtypes. The finding that patients mobilized cells with an impaired proliferation to PWM during and after adrenalin infusion has possible clinical relevance for HIV infected patients during pathological stressful conditions, such as sepsis, surgery and burns. However, this study did not find a correlation between impaired proliferation and telomeres. It is concluded that physiological stress further aggravates the HIV-induced immune deficiency.
Søndergaard
S R
SR
Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark. sisse-rs@rh.dk
Essen
M V
MV
Schjerling
P
P
Ullum
H
H
Pedersen
B K
BK
D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D001769:Blood; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000648:ultrastructure; D018414:CD8-Positive T-Lymphocytes / Q000648:ultrastructure; D002465:Cell Movement; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure*; D016615:Telomere / Q000648:ultrastructure*; D019562:Viral Load
D004837:Epinephrine