title: ISMmy1, a novel insertion sequence of Mycoplasma mycoides subsp. mycoides small colony type.
abstract: A new insertion sequence, ISMmy1, has been identified in the bovine pathogen Mycoplasma mycoides subsp. mycoides biotype small colony (MmymySC). The occurrence of ISMmy1 in 15 MmymySC strains and 12 other mycoplasmas was examined by Southern blotting. All MmymySC strains showed identical hybridisation patterns except for the type strain PG1(T), the vaccine strain T1Sr49, and the strain Afadé, which all had unique patterns. ISMmy1-like sequences were also found in the bovine pathogen Mycoplasma bovis strain Donetta(T) while mycoplasmas that are phylogenetically closer to MmymySC lack ISMmy1. This observation suggests horizontal transfer between MmymySC and M. bovis.
authors.affiliation: Department of Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Joakim
authors.initials: J
authors.lastname: Westberg
authors.forename: Anja
authors.initials: A
authors.lastname: Persson
authors.forename: Bertil
authors.initials: B
authors.lastname: Pettersson
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.forename: Karl Erik
authors.initials: KE
authors.lastname: Johansson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D016415:Sequence Alignment
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009841:Oligonucleotides