title: The effect of different morphological sampling criteria on the fraction of bursting cells recorded in the rat subiculum in vitro.
abstract: In spite of the large variability of the fraction of bursting cells reported in the subiculum (from 54 to 100%), this structure has been considered as intrinsically bursting. Here, using visually assisted whole-cell recordings and Neurobiotin labeling in vitro, we correlated the electrophysiological firing modes of subicular cells (bursting, regular-spiking and fast-spiking) with their morphological characteristics (somatic size and shape). We then examined how different morphological sampling criteria for patching affect cell classification. We found a dramatic variability in the fraction of bursting cells, which ranged from 30 to 76% depending on the sampling criteria. We discuss the implications of these findings for the notion of the subiculum as an intrinsically bursting structure.
authors.affiliation: Brain Mapping Unit, Instituto Pluridisciplinar, Universidad Complutense de Madrid, Paseo Juan XXIII, 1, Madrid 28040, Spain. liset@pluri.ucm.es
authors.forename: L
authors.initials: L
authors.lastname: Menendez de la Prida
authors.forename: F
authors.initials: F
authors.lastname: Suarez
authors.forename: M A
authors.initials: MA
authors.lastname: Pozo
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives*; D048429:Cell Size / Q000502:physiology; D004594:Electrophysiology / Q000379:methods*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000145:classification / Q000166:cytology / Q000502:physiology*; D017966:Pyramidal Cells / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015983:Selection Bias; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: C070614:neurobiotin; D001710:Biotin