title: In-vitro circadian rhythm of murine bone marrow progenitor production.
abstract: Hematopoietic processes display 24h rhythms both in rodents and in human beings. We hypothesized these rhythms to be in part generated by a circadian oscillator within the bone marrow. The ability of murine bone marrow granulo-monocytic (GM) precursors to form colonies following colony-stimulating factor (rm GM-CSF) exposure was investigated in liquid culture samples obtained every 3 h for a span of up to 198 h. The CFU-GM count varied rhythmically over the first 4 d of culture, with a reproducible maximum in the early morning hours, similar to that observed in vivo. These experiments provide the first evidence that bone marrow progenitors sustain in vitro circadian rhythmicity, and they demonstrate the presence of a circadian time-keeping system within these cells. The results support the potential usefulness of bone marrow cultures for investigating chronopharmacologic effects of anticancer drugs and cytokines on this target system.
authors.affiliation: Centre de Transfusion Sanguine des Armées, Laboratoire Immunologie Cellulaire, Clamart, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bourin
authors.forename: Arnaud F
authors.initials: AF
authors.lastname: Ledain
authors.forename: Jacques
authors.initials: J
authors.lastname: Beau
authors.forename: Dominique
authors.initials: D
authors.lastname: Mille
authors.forename: Francis
authors.initials: F
authors.lastname: Lévi
mesh_terms: D000818:Animals; D019454:Chronotherapy; D002940:Circadian Rhythm / Q000502:physiology*; D003114:Colony-Forming Units Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor