title: Genome scanning of the HXB/BXH sets of recombinant inbred strains of the rat for quantitative trait loci associated with conditioned taste aversion.
abstract: In the current study, we searched for quantitative trait loci (QTL) responsible for a conditioned taste aversion (CTA) measured as a decrease in the intake of a saccharin conditioned stimulus followed by an i.p. injection of 0.15 M LiCl (lithium chloride) (2 ml/100 g body weight). A genome scanning for QTL associated with CTA was performed in the HXB/BXH sets of recombinant inbred (RI) strains derived from the Brown Norway (BN-Lx) rat and the spontaneously hypertensive rat (SHR). The BN-Lx progenitor showed a significantly stronger CTA (8.3+/-2.8%) than the SHR progenitor (27.8+/-3.3%, p < .0001). The distribution of CTA values among RI strains was continuous, suggesting a polygenic mode of inheritance. Genome scanning of RI strains with more than 700 gene markers revealed a significant association of CTA with the D2Cebr11s4 marker on chromosome 2 (LRS = 22.7) and with the D4Cebrp149s8 marker on chromosome 4 (LRS = 23.4). The chromosome 2 putative QTL was confirmed by detecting a significant difference in CTA between the SHR progenitor (27.8+/-3.3%) and the SHR-2 (SHR.BN-D2Rat171/D2Arb24) congenic strain (13.1+/-4.4%, p < .01) that are genetically identical except for a segment of chromosome 2 that was transferred onto the genetic background of the SHR from the BN strain.
authors.affiliation: Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
authors.forename: Edita
authors.initials: E
authors.lastname: Bielavská
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kren
authors.forename: Alena
authors.initials: A
authors.lastname: Musilová
authors.forename: Václav
authors.initials: V
authors.lastname: Zídek
authors.forename: Michal
authors.initials: M
authors.lastname: Pravenec
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D002874:Chromosome Mapping; D003214:Conditioning, Classical / Q000502:physiology*; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D019655:Quantitative Trait, Heritable; D051381:Rats; D011914:Rats, Inbred BN; D011918:Rats, Inbred SHR; D011995:Recombination, Genetic / Q000235:genetics*; D013649:Taste / Q000235:genetics*
chemical_list: D005819:Genetic Markers