title: Towards the crystal structure of glycerol dehydrogenase from Thermotoga maritima.
abstract: The NAD(+)-dependent glycerol dehydrogenase (EC 1.1.1.6) from the extremely thermophilic bacterium Thermotoga maritima has been crystallized in the presence of glycerol by the hanging-drop vapour-diffusion method using 2-methyl-2,4-pentanediol (MPD) as the precipitating agent. Crystals of the enzyme complexed with NAD(+) have also been obtained. The crystals belong to the tetragonal system with space group I422 and unit-cell parameters a = 105.3, c = 134.5 A. They diffract to a maximum resolution of 1.4 A using synchrotron radiation (lambda = 0.838 A). Crystals of the enzyme-NAD(+) complex diffract to 2.5 A resolution using in-house Cu Kalpha radiation.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Vasundara
authors.initials: V
authors.lastname: Srinivasan
authors.forename: Kesen
authors.initials: K
authors.lastname: Ma
authors.forename: Michael W W
authors.initials: MW
authors.lastname: Adams
authors.forename: M Gary
authors.initials: MG
authors.lastname: Newton
authors.forename: John P
authors.initials: JP
authors.lastname: Rose
authors.forename: Bi Cheng
authors.initials: BC
authors.lastname: Wang
mesh_terms: D018360:Crystallography, X-Ray; D011487:Protein Conformation; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D020124:Thermotoga maritima / Q000201:enzymology*
chemical_list: D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase