[Effect of different estrogen receptor subtypes on vascular endothelial growth factor protein expression in human breast cancer samples].
OBJECTIVE
To evaluate the relationship of vascular endothelial growth factor expression with estrogen receptor (ER) subtypes in fresh human breast cancer samples, and study the potential effect of ER subtypes on tumor angiogenesis.


METHODS
Western blot was used to detect the VEGF and ERbeta protein expression in 86 fresh samples of human breast cancer. ERalpha was analyzed by immunohistochemistry routinely.


RESULTS
Among 86 samples, 42 (48.8%) showed low expressed VEGF and 44 (51.2%) high expressed VEGF. The level of VEGF protein was correlated with ERbeta. In high expressed VEGF group, ERbeta was also highly expressed (chi(2) = 7.36, P < 0.01). But there was no significant difference between VEGF protein level and ERalpha (P > 0.05).


CONCLUSIONS
In human breast cancer samples, VEGF may be related to ERbeta protein expression.
Wen
Xianfeng
X
Department of Breast Surgery, Cancer Hospital, Fudan University, Shanghai 200032, China.
Shen
Zan
Z
Shao
Zhimin
Z
Shen
Zhenyu
Z
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D016228:Endothelial Growth Factors / Q000378:metabolism*; D047629:Estrogen Receptor beta; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000378:metabolism*; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000145:classification / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D047629:Estrogen Receptor beta; D008222:Lymphokines; D011960:Receptors, Estrogen; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors