Separation and purification of Echis coloratus venom and some biological and biochemical effects of the proteins.
Crude venom of Echis coloratus was separated into seven protein fractions using 7% preparative native polyacrylamide gel electrophoresis. The effect of crude venom and seven venom protein fractions (F1-F7) from Echis coloratus on key metabolic activities of fibroblast cultures was investigated. Confluent cultures were incubated with the venom proteins for 3 h at 37 degrees C. The specific activity of phosphofructokinase, was significantly lowered upon incubation with the crude venom and with fractions 2, 3, 4 and 6. Citrate synthase activity was significantly lowered by the crude venom and by fractions 2 and 3. Glycogen phosphorylase activity was significantly increased by the crude venom and by fractions 2, 3, 4 and 6 leading to a significant concurrent drop in glycogen content. Creatine kinase activity was significantly increased by the crude venom and by fractions 3, 4, 5 and 6. Cellular ATP levels rose significantly upon incubation with the crude venom and with fractions 3, 4, 5 and 6. Incubation of cell sonicates with all the venom proteins did not significantly alter the activity or content of any of the studied parameters.
Al-Saleh
S S
SS
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. khanSamiullah@hotmail.com
Ghneim
H K
HK
Haddad
H Y
HY
Khan
S U
SU
D000818:Animals; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008297:Male; D051379:Mice; D011506:Proteins / Q000302:isolation & purification* / Q000502:physiology; D014757:Viper Venoms / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D017819:Viperidae
D011506:Proteins; D014757:Viper Venoms