title: Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients.
abstract: A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Boór
authors.forename: Z
authors.initials: Z
authors.lastname: Rónai
authors.forename: Z
authors.initials: Z
authors.lastname: Nemoda
authors.forename: P
authors.initials: P
authors.lastname: Gaszner
authors.forename: M
authors.initials: M
authors.lastname: Sasvári-Székely
authors.forename: A
authors.initials: A
authors.lastname: Guttman
authors.forename: H
authors.initials: H
authors.lastname: Kalász
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D036103:Nanotechnology; D017448:Receptors, Dopamine D2 / Q000235:genetics*; D050638:Receptors, Dopamine D4; D019966:Substance-Related Disorders / Q000235:genetics*
chemical_list: D017931:DNA Primers; C493599:DRD4 protein, human; D017448:Receptors, Dopamine D2; D050638:Receptors, Dopamine D4