Degradation of lignocellulose by extracellular enzymes produced by Thermomonospora fusca BD25.
Degradation products from the addition of extracellular enzymes from Thermomonospora fusca BD25 to ball-milled wheat straw, oat spelt xylan and solubilised kraft pulps were characterised by HPLC and TLC. Overall, a high percentage hydrolysis of oat spelt xylan (28.9%) occurred after 26 h incubation. However, the rates of hydrolysis of ball-milled wheat straw and kraft pulp were approximately 4-6-fold less than xylan hydrolysis, although the total percentage hydrolysis of available substrate was similar (22.2% and 25.9% respectively). Incubation of kraft pulp and ball-milled wheat straw by crude extracellular enzymes of T. fusca BD25 resulted in the detection of aromatic compounds at concentrations of 0.6 microg ml(-1) and 8.7 microg ml(-1), respectively. Hydrolysis of oat spelt xylan by T. fusca BD25 extracellular enzymes yielded a mixture of xylose, xylotriose and putative substituted-xylotriose, while the products of ball-milled wheat straw hydrolysis were xylose, glucose and a small oligomer present in the digest. The results highlight the ability of culture supernatant from T. fusca to release both simple sugars and aromatic compounds from lignocellulosic substrates and suggest a role for this organism in the biobleaching of pulp.
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Mersin Universitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, Ciftlikköy Kampüsü, 33342 Mersin, Turkey.
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D000192:Actinomycetales / Q000201:enzymology*; D001673:Biodegradation, Environmental; D002482:Cellulose / Q000032:analysis / Q000378:metabolism*; D005947:Glucose / Q000096:biosynthesis; D008031:Lignin / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D005947:Glucose