title: Immunohistological localization of the myelinating cell-specific receptor LP(A1).
abstract: LP(A1) (also termed Edg-2 or VZG-1) is a G-protein-coupled receptor for lysophosphatidic acid and its gene transcripts have been found selectively expressed by mature myelin-producing cells. We have raised in rabbit a polyclonal antibody against a sequence unique to LP(A1) and common to rat, mouse, and human orthologues. In Western blots, LP(A1) immunoreactivity appeared as 44-53 kDa bands in extracts from recombinant RH7777 cells expressing LP(A1), mouse purified oligodendrocytes, or human white matter, but not from wild-type RH7777 cells or purified astrocytes. In glial cultures, LP(A1) immunoreactivity was restricted to oligodendrocytes, appeared at cell membrane and processes, colocalized with myelin basic protein, and appeared before myelin/oligodendrocyte glycoprotein. In slices of rat and human brains, LP(A1) immunoreactivity was found in myelinated tracts, as well as in oligodendrocyte somata and their myelinating fibers. Immunoreactivities of LP(A1) and myelin basic protein colocalized in the brain, but oligodendrocyte soma showed stronger signals for LP(A1) than myelinated fibers, whereas the reverse was true for myelin basic protein. These results strengthen the view that LP(A1) is involved in myelin formation or maintenance.
authors.affiliation: Laboratoire d'Anatomie Pathologique, Hôpital Sainte Anne, Paris, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Cervera
authors.forename: Marilyn
authors.initials: M
authors.lastname: Tirard
authors.forename: Sonia
authors.initials: S
authors.lastname: Barron
authors.forename: Julien
authors.initials: J
authors.lastname: Allard
authors.forename: Suzanne
authors.initials: S
authors.lastname: Trottier
authors.forename: Joëlle
authors.initials: J
authors.lastname: Lacombe
authors.forename: Catherine
authors.initials: C
authors.lastname: Daumas-Duport
authors.forename: Pierre
authors.initials: P
authors.lastname: Sokoloff
mesh_terms: D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002478:Cells, Cultured; D006801:Humans; D007124:Immunoenzyme Techniques; D004676:Myelin Basic Protein / Q000032:analysis / Q000276:immunology; D009836:Oligodendroglia / Q000737:chemistry* / Q000166:cytology; D011817:Rabbits; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid
chemical_list: D000906:Antibodies; D004676:Myelin Basic Protein; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid