Xylanase production by Aspergillus awamori in solid-state fermentation and influence of different nitrogen sources.
The use of purified xylan as a substrate for bioconversion into xylanases increases the cost of enzyme production. Consequently, there have been attempts to develop a bioprocess to produce such enzymes using different lignocellulosic residues. Filamentous fungi have been widely used to produce hydrolytic enzymes for industrial applications, including xylanases, whose levels in fungi are generally much higher than those in yeast and bacteria. Considering the industrial importance of xylanases, the present study evaluated the use of milled sugarcane bagasse, without any pretreatment, as a carbon source. Also, the effect of different nitrogen sources and the C:N ratio on xylanase production by Aspergillus awamori were investigated, in experiments carried out in solid-state fermentation. High extracellular xylanolytic activity was observed on cultivation of A. awamori on milled sugarcane bagasse and organic nitrogen sources (45 IU/mL for endoxylanase and 3.5 IU/mL for beta-xylosidase). Endoxylanase and beta-xylosidase activities were higher when sodium nitrate was used as the nitrogen source, when compared with peptone, urea, and ammonium sulfate at the optimized C:N ratio of 10:1. The use of yeast extract as a supplement to the these nitrogen sources resulted in considerable improvement in the production of xylanases, showing the importance of this organic nitrogen source on A. awamori metabolism.
Lemos
J L
JL
Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, RJ, Brazil.
Fontes
M C
MC
Pereira
N
N
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D027461:Beta vulgaris; D018533:Biomass; D001709:Biotechnology; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D043364:Endo-1,4-beta Xylanases; D004795:Enzyme Stability; D005285:Fermentation; D005511:Food Handling; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D008031:Lignin / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000096:biosynthesis*
D003470:Culture Media; D007220:Industrial Waste; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C026579:exo-1,4-beta-D-xylosidase; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen