[Investigation of the effect of various buffer solutions used for microinjection of gene-engineering constructs on preimplantation development of mouse embryos].
We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA x C57BL)F1 mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with MgCl2 and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions.
Popova
E A
EA
National Research Institute of Physiology, Biochemistry, and Feeding of Farm Animals, Borovsk, Kaluga Oblast', 249013 Russia.
Krivokharchenko
A S
AS
Vil'ianovich
L I
LI
D000818:Animals; D001755:Blastocyst; D001757:Blastomeres; D002021:Buffers; D004247:DNA; D005260:Female; D018014:Gene Transfer Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008845:Microinjections / Q000379:methods*
D002021:Buffers; D004247:DNA