title: Synthesis and immunofluorescence assay of a new biotinylated paclitaxel.
abstract: 7-(5'-Biotinylamidopropanoyl)paclitaxel was synthesised by chemical methods; its immunofluorescence assay and the cell uptake experiments were performed by use of human leukemia U937 cells. The results indicate that paclitaxel is arresting cell cycle at the G(2)M phase only.
authors.affiliation: Department of Chemistry, Organosilicon and Synthesis Laboratory, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC.
authors.forename: Thota
authors.initials: T
authors.lastname: Sambaiah
authors.forename: Ke Yung
authors.initials: KY
authors.lastname: King
authors.forename: Shwu Chen
authors.initials: SC
authors.lastname: Tsay
authors.forename: Nai Wen
authors.initials: NW
authors.lastname: Mei
authors.forename: Sharcon
authors.initials: S
authors.lastname: Hakimclahi
authors.forename: Yiu Kay
authors.initials: YK
authors.lastname: Lai
authors.forename: Chien Hui
authors.initials: CH
authors.lastname: Lieu
authors.forename: Jih Ru
authors.initials: JR
authors.lastname: Hwu
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008870:Microtubules / Q000378:metabolism; D017239:Paclitaxel / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000378:metabolism*; D043823:Taxoids; D014407:Tumor Cells, Cultured; D020298:U937 Cells
chemical_list: C464531:7-(5'-Biotinylamidopropanoyl)paclitaxel; D000970:Antineoplastic Agents; D005456:Fluorescent Dyes; D043823:Taxoids; D017239:Paclitaxel