Assessment of motoneuron excitability using recurrent inhibition and paired reflex depression protocols: a test of reliability.
Motor output may be regulated by both pre- and post-synaptic mechanisms. The purpose of this study was to investigate the reliability of two measurement protocols, which purport to examine spinal mechanisms responsible for gating motoneuron excitability. Nine subjects (aged 29 +/- 5 years) were tested using two soleus H-reflex protocols; 1) recurrent inhibition (RI) and 2) paired reflex depression (PRD). The dependent variable for each protocol was the peak-to-peak amplitude of the conditioned Hoffmann reflex (H-reflex). Seven trials were obtained for each subject under each condition as well as control values to assess test-retest reliability. After all trials were collected the subjects rested for at least five minutes after which the process was repeated. Each subject returned to the lab after a period of no less than 24 hours at which time the process was repeated. Protocol #1: Control reflexes (20% of maximal motor response) were obtained during quiet stance. After obtaining control trials two reflex responses were elicited which were separated by 10 ms on each trial to assess recurrent inhibition (Pierrot-Deseilligny et al., 1976; Bussel and Pierrot-Deseilligny, 1977). Protocol #2: Again a double-pulse technique was used to assess reflex activation history on motoneuron pool output (Trimble et al., 2000). This protocol utilized two reflex stimuli of the same intensity separated by 80 ms. The peak-to-peak amplitude of the control, RI conditioned and PRD conditioned H-reflexes exhibited intraclass reliability estimates of .97, .97 and .93 respectively. To achieve a reliability of rI > or = .80, it is recommended that a minimum of 2 trials be used for the RI protocol and that 4 trials be used for the PRD protocol. The results indicate that both techniques provide a means to objectively and reliably measure spinal mechanisms for gating motoneuron pool output.
Earles
D R
DR
Motor Control Laboratory, Department of Kinesiology, Program in Neural Science, Indiana University, Bloomington, IN 47405, USA. dearles@indiana.edu
Morris
H H
HH
Peng
Chao-Ying J
CY
Koceja
D M
DM
D000328:Adult; D004558:Electric Stimulation; D006181:H-Reflex / Q000502:physiology*; D006801:Humans; D009046:Motor Neurons / Q000502:physiology*; D018482:Muscle, Skeletal / Q000294:innervation / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology*; D012016:Reference Values; D015203:Reproducibility of Results; D013127:Spinal Nerves / Q000502:physiology; D013569:Synapses / Q000502:physiology*