title: Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.
abstract: BACKGROUND
  Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the production efficiency of large transgenic animals.
  
  
  RESULTS
  The linker protein, a monoclonal antibody (mAb C), is reactive to a surface antigen on sperm of all tested species including pig, mouse, chicken, cow, goat, sheep, and human. mAb C is a basic protein that binds to DNA through ionic interaction allowing exogenous DNA to be linked specifically to sperm. After fertilization of the egg, the DNA is shown to be successfully integrated into the genome of viable pig and mouse offspring with germ-line transfer to the F1 generation at a highly efficient rate: 37.5% of pigs and 33% of mice. The integration is demonstrated again by FISH analysis and F2 transmission in pigs. Furthermore, expression of the transgene is demonstrated in 61% (35/57) of transgenic pigs (F0 generation).
  
  
  CONCLUSIONS
  Our data suggests that LB-SMGT could be used to generate transgenic animals efficiently in many different species.
authors.affiliation: BioAgri Corporation-Taiwan Branch, Fl. 8-8, No. 8, Song-Chiang Rd., Taipei, Taiwan. kchang@bioagricorp.com.tw
authors.forename: Keejong
authors.initials: K
authors.lastname: Chang
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: MeiSheng
authors.initials: M
authors.lastname: Jiang
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Liu
authors.forename: Ming-Che
authors.initials: MC
authors.lastname: Wu
authors.forename: Chi-Dar
authors.initials: CD
authors.lastname: Chen
authors.forename: Chao-Kuen
authors.initials: CK
authors.lastname: Lai
authors.forename: Hsin-Lung
authors.initials: HL
authors.lastname: Lo
authors.forename: Chin-Ton
authors.initials: CT
authors.lastname: Hsiao
authors.forename: Lucy
authors.initials: L
authors.lastname: Brown
authors.forename: James
authors.initials: J
authors.lastname: Bolen
authors.forename: Hsiao-I
authors.initials: HI
authors.lastname: Huang
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Ho
authors.forename: Ping Yao
authors.initials: PY
authors.lastname: Shih
authors.forename: Chen-Wen
authors.initials: CW
authors.lastname: Yao
authors.forename: Wey-Jinq
authors.initials: WJ
authors.lastname: Lin
authors.forename: Chung-Hsi
authors.initials: CH
authors.lastname: Chen
authors.forename: Fang-Yi
authors.initials: FY
authors.lastname: Wu
authors.forename: Yi-Jen
authors.initials: YJ
authors.lastname: Lin
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Ken
authors.initials: K
authors.lastname: Wang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D006041:Goats; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D010057:Oviducts / Q000601:surgery; D012756:Sheep; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism*; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal