title: The mesenchyme expresses T cell receptor mRNAs: relevance to cell growth control.
abstract: The mesenchyme plays a crucial regulatory role in organ formation and maintenance. However, comprehensive molecular characterization of these cells is lacking. We found unexpectedly that primary mesenchyme, as well as mesenchymal cell clones, express T cell receptor (TCR)alphabeta mRNAs, lacking the variable region. Immunological and genetic evidence support the expression of a corresponding TCRbeta protein. Additionally, mRNAs encoding TCR complex components including CD3 and zeta chain are present. A relatively higher expression of the mesenchymal TCRbeta mRNA by cultured mesenchymal cell clones correlates with fast growth, whereas poorly expressing cells are slow growers and are contact inhibited. The clones that express relatively higher amount of the TCR mRNA exhibit an increased capacity to form tumors in nude mice. However, the expression of this mRNA in the mesenchyme is not per se leading to tumorigenesis, as demonstrated by primary mesenchyme that does not form tumors in mice while expressing moderate amounts of the TCR transcripts. The expression of mesencymal TCRbeta was confined to the G2/M phases of the cell cycle in the MBA-13 mesenchymal cell line. This cell cycle dependent expression, considered together with the correlation between growth properties and the level of TCR expression by cell clones, implies association of mesenchymal TCR with cell growth control.
authors.affiliation: Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Mira
authors.initials: M
authors.lastname: Barda-Saad
authors.forename: Yaron
authors.initials: Y
authors.lastname: Shav-Tal
authors.forename: Arie Leon
authors.initials: AL
authors.lastname: Rozenszajn
authors.forename: Michal
authors.initials: M
authors.lastname: Cohen
authors.forename: Ayelet
authors.initials: A
authors.lastname: Zauberman
authors.forename: Asaf
authors.initials: A
authors.lastname: Karmazyn
authors.forename: Reshmi
authors.initials: R
authors.lastname: Parameswaran
authors.forename: Hadas
authors.initials: H
authors.lastname: Schori
authors.forename: Hagit
authors.initials: H
authors.lastname: Ashush
authors.forename: Avraham
authors.initials: A
authors.lastname: Ben-Nun
authors.forename: Dov
authors.initials: D
authors.lastname: Zipori
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta