title: CENP-I is essential for centromere function in vertebrate cells.
abstract: We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.
authors.affiliation: PRESTO, The Japan Science and Technology Corporation, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima, 411-8540, Shizuoka, Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Nishihashi
authors.forename: Tokuko
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiraoka
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Ikemura
authors.forename: Vinciane
authors.initials: V
authors.lastname: Regnier
authors.forename: Helen
authors.initials: H
authors.lastname: Dodson
authors.forename: William C
authors.initials: WC
authors.lastname: Earnshaw
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Fukagawa
mesh_terms: D000818:Animals; D001324:Autoantigens; D018797:Cell Cycle Proteins; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured; D002503:Centromere / Q000235:genetics* / Q000378:metabolism*; D000076247:Centromere Protein A; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D002869:Chromosome Aberrations; D020090:Chromosome Segregation; D005656:Fungal Proteins / Q000235:genetics; D008677:Metaphase / Q000502:physiology; D008870:Microtubules / Q000378:metabolism; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D012568:Schizosaccharomyces / Q000235:genetics; D029702:Schizosaccharomyces pombe Proteins; D014714:Vertebrates
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; C121933:CENPH protein, human; D018797:Cell Cycle Proteins; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; C107314:Mis6 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C060905:centromere protein C