Extraction by reversed micelles of the intracellular enzyme xylose reductase.
Xylose reductase enzyme (EC 1.1.1.21) produced by Candida guilliermondii in sugarcane bagasse was extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride cationic surfactant. An experimental design was employed to evaluate the influences of the following factors on the enzyme extraction: temperature, cosolvent, and surfactant concentration. A model was used to represent the enzyme recovery and fit of the experimental data. The extraction yielded a total recovery of 130%, and the purity increased 4.8-fold. This study demonstrates that liquid-liquid extraction by reversed micelles is a process able to recover and increase the enzymatic activity and purity of XR produced by C. guilliermondii.
Cortez
E V
EV
Faculdade de Ciências Farmacêuticas/USP, São Paulo, SP, Brazil.
Felipe
M C
MC
Roberto
I C
IC
Pessoa
A
A
Vitolo
M
M
D000449:Aldehyde Reductase / Q000096:biosynthesis / Q000302:isolation & purification*; D027461:Beta vulgaris; D018533:Biomass; D002175:Candida / Q000201:enzymology / Q000254:growth & development; D005285:Fermentation; D005511:Food Handling; D006868:Hydrolysis; D007220:Industrial Waste; D008823:Micelles; D008954:Models, Biological; D014993:Xylitol / Q000096:biosynthesis
D007220:Industrial Waste; D008823:Micelles; D000449:Aldehyde Reductase; D014993:Xylitol