A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation.
A member of the mitogen-activated protein kinase superfamily, MAK, has been proposed to have an important role in spermatogenesis, since Mak gene expression is highly restricted to testicular germ cells. To assess the biological function of MAK, we have established MAK-deficient (Mak(-/-)) mice. Mak(-/-) mice developed normally, and no gross abnormalities were observed. Spermatogenesis of the Mak(-/-) mice was also intact, and most of the mice were fertile. However, Mak(-/-) male-derived litter sizes and their sperm motility in vitro were mildly reduced. These data show that function of MAK is not essential for spermatogenesis and male fertility.
Shinkai
Yoichi
Y
Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan. yshinkai@virus.kyoto-u.ac.jp
Satoh
Hideo
H
Takeda
Naoki
N
Fukuda
Mikiko
M
Chiba
Emiko
E
Kato
Tomoya
T
Kuramochi
Takashi
T
Araki
Yoshihiko
Y
D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005298:Fertility; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000201:enzymology*
D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C081931:MAK protein, human; C509962:Mak protein, mouse