No evidence of parvovirus B19, Chlamydia pneumoniae or human herpes virus infection in temporal artery biopsies in patients with giant cell arteritis.
OBJECTIVE
Recent studies have suggested that infective agents may be involved in the pathogenesis of giant cell arteritis (GCA), in particular Chlamydia pneumoniae and parvovirus B19. We investigated temporal arteries from patients with GCA for these infections as well as human herpes viruses using the polymerase chain reaction (PCR).


METHODS
Thirty temporal artery biopsies from 30 patients suspected of having GCA within a period of 1 yr were examined. Thirteen patients had classical GCA, two had biopsy-negative GCA, 10 patients had polymyalgia rheumatica and five patients had other conditions. DNA was extracted from frozen biopsies and PCR was used to amplify genes from Chlamydia pneumoniae, parvovirus B19 and each of the eight human herpes viruses: herpes simplex viruses HSV-1 and 2, Epstein-Barr virus, cytomegalovirus, varicella zoster virus and human herpes viruses HHV-6, -7 and -8.


RESULTS
In all 30 biopsies, PCR was negative for DNAs of parvovirus B19, each of the eight human herpes viruses and C. pneumoniae.


CONCLUSIONS
We found no evidence of DNA from parvovirus B19, human herpes virus or C. pneumoniae in any of the temporal arteries. These agents do not seem to play a unique or dominant role in the pathogenesis of GCA.
Helweg-Larsen
J
J
Department of Infectious Diseases, Hvidovre University Hospital, Denmark.
Tarp
B
B
Obel
N
N
Baslund
B
B
D001706:Biopsy; D002690:Chlamydia Infections / Q000150:complications* / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000472:pathogenicity; D004247:DNA / Q000032:analysis; D013700:Giant Cell Arteritis / Q000209:etiology* / Q000473:pathology; D006564:Herpesviridae / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D006566:Herpesviridae Infections / Q000150:complications* / Q000473:pathology; D006801:Humans; D010322:Parvoviridae Infections / Q000150:complications* / Q000473:pathology; D016732:Parvovirus B19, Human / Q000235:genetics / Q000472:pathogenicity; D016133:Polymerase Chain Reaction; D011111:Polymyalgia Rheumatica / Q000150:complications / Q000473:pathology; D011446:Prospective Studies; D013699:Temporal Arteries / Q000382:microbiology* / Q000473:pathology
D004247:DNA