title: An in-vitro study of the effects of various disinfectants on prosthetic and surface materials.
abstract: OBJECTIVE
  This study assessed the effect of various disinfectants on several contaminated prosthetic and surface-covering materials.
  
  
  METHODS
  The efficacy of 6 disinfectants used at King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, on prosthetic and surface-covering materials, irreversible hydrocolloid and elastomer impression materials, wax, acrylic resin, metal, bench-covering material, and floor carpet. These materials were contaminated with Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. Counts of viable bacteria on the materials was determined by incubated replica plating on blood agar plates at 5 minute intervals. A 3 way non parametric analysis of variance was used to evaluate the main effects and interactions of the disinfectants, bacteria, and materials.
  
  
  RESULTS
  Statistical analysis showed that material, type of disinfectant, and interactions between material and bacteria were significant. Carpet has a significantly higher bacterial count than many other items (P < 0.0001) such as acrylic resin, irreversible hydrocolloid, chrome-cobalt casting, and laminated bench surfaces.
  
  
  CONCLUSIONS
  Quaternary ammonia compound and the tertiary ammonia phenol were the most effective disinfectants. Efficacy of the disinfectant depends partly on the bacteria used for contamination. Carpets in dental clinics showed high potential to retain microorganisms.
authors.affiliation: Department of Oral and Maxillofacial Rehabilitation, Faculty of Dentistry, King Abdulaziz University, PO Box 1540, Jeddah 21441, Kingdom of Saudi Arabia. sbahannan@kaau.edu.sa
authors.forename: Salma A
authors.initials: SA
authors.lastname: Bahannan
authors.forename: Maha M
authors.initials: MM
authors.lastname: Abdel-Salam
mesh_terms: D015169:Colony Count, Microbial; D003760:Dental Impression Materials; D017266:Dental Prosthesis; D004202:Disinfectants / Q000627:therapeutic use*; D005430:Floors and Floorcoverings; D006801:Humans
chemical_list: D003760:Dental Impression Materials; D004202:Disinfectants