title: Comparative study of transfection efficiency of cationic cholesterols mediated by liposomes-based gene delivery.
abstract: To develop the efficient non-viral vector for gene delivery, we compared transfection activities of cationic cholesterol derivatives. We found that the stability of the liposome-DNA complex in the presence of endosome deeply related to the transfection efficiency. We also found that the introduction of a hydrophilic group to the amino terminal of the cholesterol derivative decreased stability and facilitated the release of DNA from the endosome, resulting in higher transfection efficiency.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, 467-8603, Nagoya, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Naohide
authors.initials: N
authors.lastname: Hirashima
authors.forename: Mamoru
authors.initials: M
authors.lastname: Nakanishi
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002412:Cations; D002784:Cholesterol / Q000008:administration & dosage* / Q000737:chemistry; D008081:Liposomes; D051379:Mice; D013050:Spectrometry, Fluorescence; D014162:Transfection
chemical_list: D002412:Cations; D008081:Liposomes; D002784:Cholesterol