title: New cross-linked and sulfated derivatives of partially deacetylated hyaluronan: synthesis and preliminary characterization.
abstract: Partial chemical deacetylation of hyaluronan (HA) has been carried out using known procedures and carefully controlled experimental conditions in order to minimize chain degradation. The sample described herein (deHA) has a degree of deacetylation of about 17%, which corresponds to what required for its further use, but a molecular weight of about 1/25 with respect to the native, starting material. Chemical gels have been prepared with different degrees of cross-linking by means of a Ugi multicomponent condensation reaction involving aqueous deHA, formaldehyde, and cyclohexylisocyanide: the gels are mechanically stable and exhibit good water uptake strongly dependent on the extent of cross-linking, as expected. deHA samples have also been selectively N-sulfated or O-sulfated: the former exhibit anticoagulant properties well exceeding those of the latter and not too inferior to heparin.
authors.affiliation: Department of Chemistry, University La Sapienza, Rome, Italy. vittorio.crescenzi@uniroma.it
authors.forename: Vittorio
authors.initials: V
authors.lastname: Crescenzi
authors.forename: Andrea
authors.initials: A
authors.lastname: Francescangeli
authors.forename: Davide
authors.initials: D
authors.lastname: Renier
authors.forename: Davide
authors.initials: D
authors.lastname: Bellini
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013431:Sulfates / Q000737:chemistry; D014783:Viscosity
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D013431:Sulfates; D006820:Hyaluronic Acid