title: Testing mouse mammary tumor virus superantigen as adjuvant in cytotoxic T-lymphocyte responses against a melanoma tumor antigen.
abstract: Cytotoxic T cells represent a powerful strategy for antitumor treatment. Depending on the route of injection, an important role for CD4 T cell-mediated help was observed in the induction of this response. For this reason, we investigated whether induction of a CTL response to the HLA-A2-restricted immunodominant peptide melanoma antigen Melan-A was improved by using rVVs expressing the CTL-defined epitope alone or in combination with an SAg. In the latter case, the few infected dendritic cells simultaneously presented an SAg and an antigen, i.e., peptide. Here, we show that the anti-Melan-A response was efficiently induced but not significantly improved by coexpression of the SAg.
authors.affiliation: Institute of Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Susanne
authors.initials: S
authors.lastname: Wirth
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bille
authors.forename: Sylvain
authors.initials: S
authors.lastname: Koenig
authors.forename: Nathalie
authors.initials: N
authors.lastname: Wehrli
authors.forename: Isabelle
authors.initials: I
authors.lastname: Miconnet
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lévy
authors.forename: Heidi
authors.initials: H
authors.lastname: Diggelmann
authors.forename: Pedro
authors.initials: P
authors.lastname: Romero
authors.forename: Hans
authors.initials: H
authors.lastname: Acha-Orbea
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D015234:HLA-A Antigens / Q000235:genetics; D015789:HLA-A2 Antigen; D008164:Luminescent Proteins / Q000235:genetics; D058965:MART-1 Antigen; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D008545:Melanoma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins; D018089:Superantigens / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D000276:Adjuvants, Immunologic; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D008164:Luminescent Proteins; D058965:MART-1 Antigen; C548668:Mlana protein, mouse; D009363:Neoplasm Proteins; D011993:Recombinant Fusion Proteins; D018089:Superantigens; D049452:Green Fluorescent Proteins