[Effects of repeated +Gz exposures on the expression of c-fos protein in rabbit brain].
OBJECTIVE
To investigate the effect of repeated +Gz exposures on the expression and distribution of c-fos protein immunoreactivity in rabbit brain.


METHODS
Twenty rabbits were divided randomly into 4 groups (n=5 in each group). Animals were anesthetized and then exposed to +4 Gz until 30 s after the arterial pressure at eye level dropped to 0 kPa. The exposure was repeated for 3 times with 30 min intervals. The expression of c-fos protein in rabbit brain was examined at 0 h, 1 h and 6 h after +Gz exposure by immunohistochemistry method.


RESULTS
c-fos protein was obviously expressed in cortex, the third ventricle, hippocampus and dentate gyrus immediately after repeated +Gz exposures, and strongly expressed 1 h after the exposure, and then had a tendency to decrease 6 h after the exposure.


CONCLUSIONS
Three +Gz exposures may cause time-dependent c-fos expression in rabbit brain, which may be involved in +Gz-induced brain damage.
Sun
Xi-qing
XQ
Department of Aerospace Biodynamics, The Fourth Military Medical University, Xi'an, China. sunxiqing@163.net
Li
Jing
J
Wu
Xing-yu
XY
D000337:Aerospace Medicine; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002540:Cerebral Cortex / Q000378:metabolism / Q000502:physiology; D018891:Dentate Gyrus / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology; D018471:Hypergravity; D007150:Immunohistochemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D020542:Third Ventricle / Q000378:metabolism / Q000502:physiology; D013997:Time Factors
D016760:Proto-Oncogene Proteins c-fos