Maize replicative alpha-type DNA polymerase: separation of polymerase and primase activities and recognition of primase subunits.
DNA polymerase and DNA primase activities in the maize alpha-type DNA polymerase 2 were dissociated and DNA polymerase-free DNA primase was studied. DNA primase synthesized primers that were 8-34 nucleotides long, with more intense bands at 15-17 nucleotides in length. DNA polymerase 1 (a putative delta-type enzyme) or DNA polymerase 2 were assayed after template-priming with purified DNA primase and showed a differential use of templates: whereas DNA polymerase 2 used a polydT template more efficiently than a natural template, DNA polymerase 1 used both of them poorly. The molecular size of DNA primase was estimated to be 68 kDa by gel filtration, western blotting and by a DNA primase 'trapping' assay.
García
Elpidio
E
aDepartamento de Bioquímica, Facultad de Química, UNAM, Ave. Universidad y Copilco, México DF, 04510, México bREGER, UMR 50 97 Université Bordeaux-CNRS, bat 3a-3eme étage, 146 rue Leo-Saignat, F-33076, Bordeaux Cedex, France.
Laquel
Patricia
P
Castroviejo
Michel
M
Plasencia
Javier
J
Vázquez-Ramos
Jorge M
JM