Isolation of an Acinetobacter sp. ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole.
Acinetobacter sp. strain ST-550 was isolated from fumus soil as an efficient indigo producer in the presence of organic solvent. The minimum inhibitory concentration of indole was 0.4 mg/ml for ST-550. ST-550 produced only a small amount of indigo (less than 0.01 microg/ml) when grown in the presence of indole at concentrations of 0.05 to 0.3 mg/ml without any organic solvent. However. ST-550 produced indigo effectively when grown in the presence of a large volume of diphenylmethane and a high level of indole: optimized conditions were 3 ml of a medium containing 0.3 ml diphenylmethane and 2.7 mg indole. Under these conditions, ST-550 produced 0.88 mg indigo (292 microg/mI medium).
Doukyu
N
N
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Nakano
T
T
Okuyama
Y
Y
Aono
R
R
D000150:Acinetobacter / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D005285:Fermentation; D007203:Indigo Carmine; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012997:Solvents
D003470:Culture Media; D007211:Indoles; D012997:Solvents; D007203:Indigo Carmine