title: Synthesis of solid-supported mirror-image sugars: a novel method for selecting receptors for cellular-surface carbohydrates.
abstract: We introduced a novel method, through mirror-image phage display, for the identification of high-affinity D-peptides to target specific cell-surface carbohydrates. Both 3-deoxy-alpha-L-manno-2-octulosonic acid (L-KDO) and L-sialic acid and an L-sialo-disaccharide have been synthesized and attached to a solid support for selection of high-affinity peptide binders displayed on phages. Our initial studies in this effort produce single-chain Fab sequences and dodecapeptides that bind to sialic acid and KDO with nanomolar and high micromolar affinity.
authors.affiliation: Department of Chemistry, The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA.
authors.forename: I A
authors.initials: IA
authors.lastname: Kozlov
authors.forename: S
authors.initials: S
authors.lastname: Mao
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.forename: L
authors.initials: L
authors.lastname: Lee
authors.forename: P S
authors.initials: PS
authors.lastname: Sears
authors.forename: C
authors.initials: C
authors.lastname: Gao
authors.forename: A R
authors.initials: AR
authors.lastname: Coyle
authors.forename: K D
authors.initials: KD
authors.lastname: Janda
authors.forename: C H
authors.initials: CH
authors.lastname: Wong
mesh_terms: D000595:Amino Acid Sequence; D000915:Antibody Affinity; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D002846:Chromatography, Affinity; D007135:Immunoglobulin Variable Region / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D001704:Biopolymers; D002241:Carbohydrates; D007135:Immunoglobulin Variable Region; D011956:Receptors, Cell Surface