title: [Quantitation of hepatitis C virus RNA in amniotic fluid of gravida infected by hepatitis C virus].
abstract: OBJECTIVE
  To detect hepatitis C virus (HCV) RNA in amniotic fluid of gravida and investigate mother-to-infant transmission of HCV.
  
  
  METHODS
  Thirty-four HCV seropositive gravida (experimental group) were engaged. Fluorescence quantitative polymerase chain reaction (PCR) based on amplisensor assay and reverse transcription -PCR (RT-nPCR) was used. Serum HCV RNA positive sera were genotyped by RFLP analysis of PCR products from 5'NC region. Sera and amniotic fluid samples of 40 normal gravida were set as the control group.
  
  
  RESULTS
  In the experimental group, HCV RNA was detected in amniotic fluid (5.9%, 2/34) of 2 cases. HCV RNA titers were 10(5) and 10(6) copy/ml respectively. No HCV RNA was detected in the amniotic fluid and sera of the control (n = 40).
  
  
  CONCLUSIONS
  HCV RNA was rarely detected in amniotic fluid. The amniotic fluid is not the main route of HCV mother-to-infant transmission.
authors.affiliation: Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Nie
authors.forename: Pingzhong
authors.initials: P
authors.lastname: Wang
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000821:virology*; D005260:Female; D005838:Genotype; D016174:Hepacivirus; D006526:Hepatitis C / Q000635:transmission* / Q000821:virology; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis*
chemical_list: D012367:RNA, Viral