Comparison of responses elicited by immunization with a Legionella species common lipoprotein delivered as naked DNA or recombinant protein.
To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD19+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
Yoon
Won Suck
WS
Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul, Korea.
Park
Seol Hee
SH
Park
Yong Keun
YK
Park
Seung Chul
SC
Sin
Jeong Im
JI
Kim
Min Ja
MJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D029968:Escherichia coli Proteins; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007875:Legionella / Q000145:classification / Q000276:immunology*; D016952:Legionella pneumophila / Q000276:immunology; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000235:genetics / Q000276:immunology*; D011509:Proteoglycans; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D007074:Immunoglobulin G; D008074:Lipoproteins; D010457:Peptidoglycan; D011509:Proteoglycans; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C069988:PplA protein, Legionella pneumophila