Process chromatography: current constraints and future options for the adsorptive recovery of bioproducts.
A contemporary review of adsorption chromatography must embrace aspects of fixed-bed, batch suspension and fluidised-bed contacting of complex feedstocks with adsorbents chemically derivatised with ligands with binding specificities for target bioproducts. Advances in the design of adsorbents, ligands and contactors have facilitated opportunities for integrated processing of unclarified feedstocks to benefit purity and yield of macromolecular products. In the face of competition from apparently simpler, yet productive, technologies (e.g. precipitation, crystallisation and aqueous solvent extraction), further advances in chromatographic purification of macromolecules and nanoparticulates demand close collaboration between inventors and/or manufacturers of new products and the suppliers of chromatographic hardware and consumables.
Lyddiatt
Andrew
A
Biochemical Recovery Group, Research Centre for Formulation Engineering, School of Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. a.lyddiatt@bham.ac.uk
D000327:Adsorption; D001688:Biological Products / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D019149:Bioreactors / Q000191:economics; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D005544:Forecasting; D008024:Ligands; D046911:Macromolecular Substances; D036103:Nanotechnology / Q000379:methods / Q000639:trends
D001688:Biological Products; D008024:Ligands; D046911:Macromolecular Substances