title: Inhibition of ultraviolet B radiation-induced interleukin 10 expression in murine keratinocytes by selenium compounds.
abstract: BACKGROUND
  Selenium is an essential trace nutrient necessary for the normal function of the immune system. Selenium compounds protect mice against ultraviolet (UV) B-induced tumours, probably by preventing oxidative damage to the host skin cells and to the host immune system. One possible mechanism of protection is that selenium can prevent oxidative stress-induced release of cytokines such as interleukin (IL)-10, which could suppress cell-mediated immunity.
  
  
  OBJECTIVE
  To determine whether selenium compounds can inhibit UVB induction of IL-10 protein in murine keratinocytes.
  
  
  METHODS
  The murine keratinocyte cell line PAM 212 was treated with or without selenomethionine (50-200 nmol L-1) or sodium selenite (1-50 nmol L(-1)) for 24 h before exposure to 200 J m(-2) UVB. The cells were stained with an antibody to IL-10, 24 h after irradiation.
  
  
  RESULTS
  Preincubation with both selenium compounds inhibited UVB induction of IL-10 immunostaining, although selenomethionine was more effective. Pretreatment with 200 nmol L(-1) selenomethionine decreased IL-10 immunostaining to levels seen in the unirradiated controls.
  
  
  CONCLUSIONS
  The protective effects of selenium against UVB-induced skin cancer in murine models may result, in part, from its ability to inhibit release of cytokines that are capable of suppressing cell-mediated immunity.
authors.affiliation: Department of Dermatology, University of Edinburgh, Royal Infirmary of Edinburgh, Lauriston Building, Edinburgh EH3 9YW, UK.
authors.forename: T S
authors.initials: TS
authors.lastname: Rafferty
authors.forename: C
authors.initials: C
authors.lastname: Walker
authors.forename: J A A
authors.initials: JA
authors.lastname: Hunter
authors.forename: G J
authors.initials: GJ
authors.lastname: Beckett
authors.forename: R C
authors.initials: RC
authors.lastname: McKenzie
mesh_terms: D000818:Animals; D002460:Cell Line; D007111:Immunity, Cellular / Q000187:drug effects; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000276:immunology*; D015603:Keratinocytes / Q000276:immunology* / Q000528:radiation effects*; D051379:Mice; D018036:Selenium Compounds / Q000627:therapeutic use*; D012645:Selenomethionine / Q000627:therapeutic use; D018038:Sodium Selenite / Q000627:therapeutic use; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D018036:Selenium Compounds; D016753:Interleukin-10; D012645:Selenomethionine; D018038:Sodium Selenite