Immunosensors for pesticide analysis: antibody production and sensor development.
Immunosensors, a type of affinity biosensor, are based on the binding interactions between an immobilized biomolecule (antibody/antigen) on the electronic transducer surface with the analyte of interest (antigen/antibody), resulting in a detectable signal. The sensor system takes advantage of the high selectivity provided by the molecular recognition characteristic of an antibody, which binds reversibly with a specific antigen. This review article presents the current status of immunosensors, highlighting their potential benefits and limitations for pesticide analysis. The basic criteria for generating specific antibodies against low-molecular-mass pesticides, which are usually nonimmunogenic in nature, are briefly discussed. The article also describes the fundamentals of important transducer technologies and their use in immunosensor development.
Raman
Suri C
SC
Institute of Microbial Technology, Chandigarh, India. crsuri@yahoo.com
Raje
Manoj
M
Varshney
Grish C
GC
D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D004563:Electrochemistry; D004867:Equipment Design; D010575:Pesticides / Q000032:analysis* / Q000276:immunology
D010575:Pesticides