High-resolution 1H NMR spectroscopy in rat liver using magic angle turning at a 1 Hz spinning rate.
It is demonstrated that a high-resolution (1)H NMR spectrum of excised rat liver can be obtained using the technique of magic angle turning (MAT) at a sample spinning rate of 1 Hz. A variant of the phase-corrected MAT (PHORMAT) pulse sequence that includes a water suppression segment was developed for the investigation. The spectral resolution achieved with PHORMAT approaches that obtained from a standard magic angle spinning (MAS) experiment at a spinning rate of several kHz. With such ultra-slow spinning, tissue and cell damage associated with the standard MAS experiment is minimized or eliminated. The technique is potentially useful for obtaining high-resolution (1)H spectra in live animals.
Hu
Jian Zhi
JZ
Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Rommereim
Donald N
DN
Wind
Robert A
RA
D000818:Animals; D008099:Liver / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344