A cell-culture system for long-term maintenance of elevated hydrostatic pressure with the option of additional tension.
In cell stress research, there is still a need to apply long-term hydrostatic pressure without changing any other environmental condition. We present here a new, open, pressurized chamber system allowing long-term sustained and dynamic application of hydrostatic pressure with the option of additional tension. Based on the computer-controlled Flexcell Strain Unit, we designed a pressurized chamber with a dynamic airflow and a defined membrane extension, which can be regulated by spacers. During operation up to 26.6kPa, O(2) partial pressures and pH in the cell-culture medium do not change compared to control cultures kept at normal atmosphere.
Hasel
Cornelia
C
Department of Pathology, Ulm University, Albert-Einstein-Allee 11, D-89089 Ulm, Germany.
Dürr
Susanne
S
Brüderlein
Silke
S
Melzner
Ingo
I
Möller
Peter
P
D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D010100:Oxygen; D010313:Partial Pressure; D013312:Stress, Physiological / Q000378:metabolism; D013500:Surface Tension
D010100:Oxygen