Azotobacter chroococcum does not contain sodA or its gene product Mn-superoxide dismutase.
Azotobacter chroococcum and Azotobacter vinelandii grown in Burk medium with 1% mannitol (BM) or in BM supplemented with 2.2 mg/mL ammonium acetate (BM+N) were found to have only iron-containing and CuZn-containing superoxide dismutase. Furthermore, genomic DNA from A. chroococcum and A. vinelandii were subjected to polymerase chain reaction analysis using sodA- and sodB-specific primers and yielded only a sodB product. These results dispute the assertion by Buchanan and Lees (Can. J. Microbiol. 26: 441-447, 1980) that A. chroococcum contains Mn-superoxide dismutase.
Caldwell
Jane M
JM
Department of Microbiology, North Carolina State University, Raleigh 27695-7615, USA.
Hassan
Hosni M
HM
D000595:Amino Acid Sequence; D001395:Azotobacter / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000032:analysis* / Q000235:genetics
D013482:Superoxide Dismutase