Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase.
In this paper a novel and general procedure is presented for detection of organophosphate-inhibited human butyrylcholinesterase (HuBuChE), which is based on electrospray tandem mass spectrometric analysis of phosphylated nonapeptides obtained after pepsin digestion of the enzyme. The utility of this method is exemplified by the positive analysis of serum samples from Japanese victims of the terrorist attack with sarin in the Tokyo subway in 1995.
Fidder
A
A
Division Chemical & Biological Protection, TNO Prins Maurits Laboratory, P.O. Box 45, 2280 AA Rijswijk, The Netherlands.
Hulst
A G
AG
Noort
D
D
de Ruiter
R
R
van der Schans
M J
MJ
Benschop
H P
HP
Langenberg
J P
JP
D002091:Butyrylcholinesterase / Q000097:blood*; D002800:Cholinesterase Inhibitors / Q000009:adverse effects*; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007306:Insecticides / Q000009:adverse effects*; D010446:Peptide Fragments / Q000097:blood; D012524:Sarin / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization; D023481:Terrorism
D002800:Cholinesterase Inhibitors; D007306:Insecticides; D010446:Peptide Fragments; D012524:Sarin; D002091:Butyrylcholinesterase