Further identification of NSF* as an epilepsy related gene.
Previous data proved that NSF* was an epilepsy related gene (ERG1). In this study, using phosphorothioate oligodeoxynucleotide (PS-ODN), an antisense of NSF to downregulate the function of NSF in vitro cultured hippocampus neurons and PC12, this treatment simultaneously induced enhancement of the neurite outgrowth of hippocampal neurons and PC12, a phenomenon similar to the structural changes following epilepsy. Immunocytochemistry analysis showed that the enhancement of neurite outgrowth was in a sequence-specific manner and Northern blot confirmed that the decrease of NSF mRNA levels in PC12 was in a dose-dependent manner. Moreover the expression of NSF was downregulated during differentiation of PC12 induced by NGF and high KCl. Therefore, providing more evidence to support the fact that NSF was an ERG1.
Yu
Furong
F
Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Science, Chinese Academy of Sciences, 320 Yue-Yang Road, 200031, People's Republic of China.
Guan
Zhuo
Z
Zhuo
Ming
M
Sun
Lanying
L
Zou
Weiguo
W
Zheng
Zhongcheng
Z
Liu
Xinyuan
X
D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D004827:Epilepsy / Q000235:genetics* / Q000378:metabolism* / Q000503:physiopathology; D005333:Fetus; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D052066:N-Ethylmaleimide-Sensitive Proteins; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism*; D009841:Oligonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D016716:PC12 Cells; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013873:Thionucleotides / Q000494:pharmacology; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D008869:Microtubule-Associated Proteins; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013873:Thionucleotides; D033921:Vesicular Transport Proteins; C086722:phosphorothioate-3'-hydroxypropylamine oligonucleotide sT2a; D020932:Nerve Growth Factor; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat