title: Transient reduction in the tRNA 4-thiouridine content induced by ultraviolet A during post-irradiation growth in Enterobacter cloacae.
abstract: The influence of previous exposure to ultraviolet-A radiation (UVA) was studied on the susceptibility of Enterobacter cloacae to undergo the growth delay effect. Comparison of growth curves corresponding to irradiated and control cells showed that a previous treatment with UVA almost abolished the growth delay effect. UV absorption spectra of tRNA, and reverse phase HPLC analysis of hydrolysed tRNA, demonstrated a low content of 4-thiouridine in E. cloacae cells grown after UVA exposure at low doses. Since 4-thiouridine is the UVA target responsible for initiation of growth delay, this observation explained the influence of previous exposure to UVA on the susceptibility of this organism to undergo growth delay. A similar but weaker alteration was found when Escherichia coli was assayed. The results suggest that, in addition to cross-linking with cytidine residues, the content of 4-thiouridine in tRNA may be modified by UVA by an unknown mechanism.
authors.affiliation: Departamento de Radiobiología, Comisión Nacional de Energía Atómica, Av. General Paz 1499, 1650 General San Martín, Buenos Aires, Argentina. oppezzo@cnea.gov.ar
authors.forename: Oscar J
authors.initials: OJ
authors.lastname: Oppezzo
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Pizarro
mesh_terms: D004307:Dose-Response Relationship, Radiation; D016972:Enterobacter cloacae / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D012329:RNA, Bacterial / Q000737:chemistry / Q000528:radiation effects*; D012343:RNA, Transfer / Q000737:chemistry / Q000528:radiation effects*; D013891:Thiouridine / Q000032:analysis* / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D012329:RNA, Bacterial; D013891:Thiouridine; D012343:RNA, Transfer