Laboratory methods for the diagnosis of asthenozoospermia.
Human sperm samples from 46 men were evaluated for standard semen parameters by two trained technicians, according to WHO criteria. A computer-aided semen analyzer (CASA, HTM-IVOS) was employed. The overall mean coefficient of variation for the 2 participants and the 46 samples studied was 17% for the percentage of progressive motile spermatozoa (r = 0.77). Twenty-nine (63%) samples were classified as asthenozoospermia by both methods. From the studied samples, 12 (26%) were classified as asthenozoospermia by the subjective method and 2 (4.3%) by CASA (p =.01). The two methods do not provide directly comparable data.
Curi
S
S
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Male Fertility Laboratory, Department of Clinical Biochemistry, University Clinical Hospital José de San Martin, Argentina.
Ariagno
J
J
Repetto
H
H
Chenlo
P
P
Mendeluk
G
G
Pugliese
N
N
Sardi
M
M
Blanco
A
A
D006801:Humans; D007091:Image Processing, Computer-Assisted; D007248:Infertility, Male / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D015588:Observer Variation; D009845:Oligospermia / Q000175:diagnosis* / Q000503:physiopathology; D015203:Reproducibility of Results; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*