Effect of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus in the rat.
The effects of intracerebralventricular (icv) injection of nitric oxide (NO) donor and nitric oxide synthase (NOS) inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus (PVN) of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method. The results showed that icv injection of NO donor sodium nitroprusside (SNP) had dose-dependent inhibitory effects on vasopressin (VP) secreting units in PVN. Icv injection of NOS inhibitor N(  )-nitro-L-arginine methyl ester (L-NAME) also inhibited all of the VP-secreting neurons examined. Both the drugs had no effect on almost all oxytocin (OT) secreting units. These results suggest that NO may differentially modulate the activities of OT and VP neurons.
Ji
Y P
YP
Department of Physiology, Xi an Medical University, Xi an 710061, China. lj509122@pub.xaonline.com
Mei
J
J
D000818:Animals; D004594:Electrophysiology; D007276:Injections, Intraventricular; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D052248:Nitric Oxide Synthase Type I; D010286:Paraventricular Hypothalamic Nucleus / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D020030:Nitric Oxide Donors; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat