Cloning and nucleotide sequence analysis of the Streptococcus sobrinus gtfU gene that produces a highly branched water-soluble glucan.
Streptococcus sobrinus has four gtf genes, gtfI, gtfS, gtfT, and gtfU, on the chromosome. These genes correspond respectively to the enzymes GTF-I, GTF-S1, GTF-S2, and GTF-S3. An Escherichia coli MD66 clone that contained the S. sobrinus gtfU gene was characterized. Immunological properties showed that the protein produced by the E. coli MD66 clone was similar to S. sobrinus GTF-S1. Biological properties and a linkage analysis of the glucans by 13C NMR spectrometry revealed that the protein produced by the E. coli MD66 clone was GTF-S1.
Hanada
Nobuhiro
N
Department of Oral Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Sinjuku-ku, Tokyo 162-8640, Japan. nhanada@nih.go.jp
Fukushima
Kazuo
K
Nomura
Yoshiaki
Y
Senpuku
Hidenobu
H
Hayakawa
Mitsuo
M
Mukasa
Hidehiko
H
Shiroza
Teruaki
T
Abiko
Yoshimitsu
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005936:Glucans / Q000096:biosynthesis / Q000737:chemistry; D005964:Glucosyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016986:Streptococcus sobrinus / Q000201:enzymology / Q000235:genetics*; D014162:Transfection
D005936:Glucans; D005964:Glucosyltransferases; C110463:glucosyltransferase C