title: Surface antigen expression on bladder tumor cells induced by bacillus Calmette-Guérin (BCG): A role of BCG internalization into tumor cells.
abstract: BACKGROUND
  The antitumor mechanisms of bacillus Calmette-Guérin (BCG) against bladder cancer is still unclear. We previously reported that BCG was internalized by and survived within murine bladder tumor cells (MBT-2) for at least 40 days. In the present study, we investigated the effect of BCG on the surface antigen expression of bladder tumor cells and the characteristics of these cells as antigen-presenting cells in vitro.
  
  
  METHODS
  Surface antigen (major histocompatibility complex (MHC) Class II, CD1, CD80 and intercellular adhesion molecule-1 (ICAM-1)) expression on BCG-treated murine (MBT-2) and human (T-24, J82) bladder tumor cells were analyzed using flow cytometry. The production of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) from murine lymphocytes sensitized with BCG or BCG-treated tumor cells were also investigated.
  
  
  RESULTS
  The expressions of MHC Class II, CD1, CD80 and ICAM-1 were augmented in all of the bladder tumor cell lines used; however, they were augmented to varying degrees among the cell lines that were treated with live BCG. Heat-killed BCG had little or no effect. When murine lymph node cells sensitized with BCG or BCG-treated MBT-2 cells were cocultured with BCG-treated MBT-2 cells, significant amounts of IL-2 and IFN-gamma were produced in the culture medium.
  
  
  CONCLUSIONS
  BCG induced the augmented expression of surface antigens, such as MHC Class II, CD1, CD80 and ICAM-1, of bladder tumor cells. Furthermore, BCG-treated MBT-2 cells could stimulate BCG-sensitized lymphocytes to produce IL-2 and IFN-gamma. These results strongly suggest that bladder tumor cells gained the characteristics and functions of antigen-presenting cells (APC).
authors.affiliation: Japan BCG Laboratory, Matsuyama, Kiyose-city, Tokyo, Japan. jbcglab@din.or.jp
authors.forename: Noriko
authors.initials: N
authors.lastname: Ikeda
authors.forename: Ichiro
authors.initials: I
authors.lastname: Toida
authors.forename: Akio
authors.initials: A
authors.lastname: Iwasaki
authors.forename: Koji
authors.initials: K
authors.lastname: Kawai
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Akaza
mesh_terms: D000276:Adjuvants, Immunologic / Q000493:pharmacokinetics*; D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D001500:BCG Vaccine / Q000493:pharmacokinetics*; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D001500:BCG Vaccine