TREX is a conserved complex coupling transcription with messenger RNA export.
The essential yeast proteins Yra1 and Sub2 are messenger RNA export factors that have conserved counterparts in metazoans, designated Aly and UAP56, respectively. These factors couple the machineries that function in splicing and export of mRNA. Here we show that both Yra1 and Sub2 are stoichiometrically associated with the heterotetrameric THO complex, which functions in transcription in yeast. We also show that Sub2 and Yra1 interact genetically with all four components of the THO complex (Tho2, Hpr1, Mft1 and Thp2). Moreover, these components operate in the export of bulk poly(A)(+) RNA as well as of mRNA derived from intronless genes. Both Aly and UAP56 associate with human counterparts of the THO complex. Together, these data define a conserved complex, designated the TREX ('transcription/export') complex. The TREX complex is specifically recruited to activated genes during transcription and travels the entire length of the gene with RNA polymerase II. Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export.
Strässer
Katja
K
BZH, University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Masuda
Seiji
S
Mason
Paul
P
Pfannstiel
Jens
J
Oppizzi
Marisa
M
Rodriguez-Navarro
Susana
S
Rondón
Ana G
AG
Aguilera
Andres
A
Struhl
Kevin
K
Reed
Robin
R
Hurt
Ed
E
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004268:DNA-Binding Proteins; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D005804:Genes, Lethal / Q000235:genetics; D006801:Humans; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C063494:HPR1 protein, S cerevisiae; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C405934:THO2 protein, S cerevisiae; D014157:Transcription Factors; C105887:YRA1 protein, S cerevisiae; C068359:MFT1 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C027900:RNA-dependent ATPase