title: The novel exon, exon T, of the human progesterone receptor gene and the genomic organization of the gene.
abstract: Recently, we have cloned the novel isoform of the progesterone receptor (PR) cDNA (PR isoform S cDNA) from the human testicular cDNA library. The isoform S cDNA consists of the novel exon (termed the exon S of the PR gene) and the exons 4-8 of the PR gene. In order to investigate the existence of the other isoform of the human PR cDNA, the human testicular cDNA library was screened by the exons 4-8 corresponding sequence of the human PR cDNA in the present study. As a result, we have identified a novel isoform of the PR cDNA (termed the PR isoform T cDNA (PR-T cDNA)), which consisted of a previously unidentified 5'-sequence and the exons 4-8 of the PR gene. The structure of this isoform T cDNA is essentially similar to that of the isoform S cDNA. By the genomic cloning, the 5'-sequence of the PR isoform T mRNA was demonstrated to originate from a novel independent exon, exon T, which was located in the 5'-upstream region of the exon S.
authors.affiliation: Department of Obstetrics and Gynecology, Yamanashi Medical University, Shimokato 1110, Tamaho, Nakakoma 409-3898, Japan. shirata@swallow.res.yamanashi-med.ac.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Hirata
authors.forename: Tomoko
authors.initials: T
authors.lastname: Shoda
authors.forename: Junzo
authors.initials: J
authors.lastname: Kato
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011980:Receptors, Progesterone / Q000235:genetics*; D013737:Testis / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D011980:Receptors, Progesterone