title: [Study on production of acrylamide by microbial method (I)--Culture of bacterium cells and expression of high activity of nitrile hydratase].
abstract: The cultural conditions for the growth of Norcardia cell were studied in this paper. Controlling pH value, adding nutrient and optimizing the quantity of inducer during cultivation, the activity of nitrile hydratase reached 6567 u/mL (culture medium), which was the highest value appeared in native journals. In the farther hydratase experiments, no by-product, crylic acid, was detected. It showed that the activity of amidase was not promoted obviously while the activity of nitrile hydratase was increased greatly. The results set a strong foundation for the industrial application and the research on new technology.
authors.affiliation: Tsinghua University, Department of Chemical Engineering, Institute of Biochemical Engineering, Beijing 100094, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
authors.forename: Zhong-Yao
authors.initials: ZY
authors.lastname: Shen
authors.forename: Jian-Xun
authors.initials: JX
authors.lastname: Zhao
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Sun
mesh_terms: D000178:Acrylamides / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D018929:Cell Culture Techniques; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009618:Nocardiaceae / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000178:Acrylamides; D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase; D005947:Glucose