[Effects of c-erbB(2) on the hCG-induced progesterone production of rat luteal cells].
The effects of c-erbB(2) antisense oligodeoxynucleotides (antisense c-erbB(2) ODN) on hCG-induced progesterone production of isolated rat luteal cells in relation to cAMP, Ca(2+) and cycloheximide (CYX) were investigated. Antisense c-erbB(2) ODN inhibited hCG-induced progesterone production in a dose-dependent manner; and the percentage of luteal cells stained for c-erbB(2) protein was decreased. But nonsense tat ODN had no similar effect. The effect of dbcAMP was in contrast with that of antisense c-erbB(2) ODN, which suppressed the progesterone production and reduced the percentage of luteal cells stained for c-erbB(2) protein. On the other hand, both verapamil and CYX enhanced the inhibition of antisense c-erbB(2) ODN. The result of the present investigation suggests that cAMP, Ca(2+)-dependent pathway participates in the expression of protooncogene.
Jin
X
X
Department of Physiology, Jiangxi Medical College, Nanchang 330006.
Fang
L
L
Zheng
Y H
YH
Zhong
Z S
ZS
Kuang
H B
HB
D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D018734:Genes, erbB-2; D008184:Luteal Cells / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D006063:Chorionic Gonadotropin; D016376:Oligonucleotides, Antisense; D011374:Progesterone