The assignment of PRKCI to bovine chromosome 1q34-->q36 by FISH suggests a new assignment to human chromosome 3.
Two bovine BAC clones were identified by PCR as containing the bovine gene PRKCI. Both clones were assigned by FISH to bands q34-->q36 on BTA1. The sequence information derived from genomic DNA and from both clones was identical and showed a high degree of homology to human PRKCI (HSAXq21.3, 95.5% homology), and mouse Prkcl (MMU3, 13.8 cM, 87.6% homology) and rat Prkcl (88.8% homology). This assignment could suggest a disruption of the synteny conservation of mammalian X-linked genes, but most likely suggests a misassignment of this gene to the human X.
De Donato
M
M
Departamento de Biomedicina, IIBCA, Universidad de Oriente, Cumana, Venezuela. marcosdedonato@yahoo.com
Gallagher
D S
DS
Davis
S K
SK
Stelly
D M
DM
Taylor
J F
JF
D000818:Animals; D002417:Cattle; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D020161:Physical Chromosome Mapping; D011493:Protein Kinase C / Q000235:genetics*; D051381:Rats; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D026801:Synteny / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics*; D000099293:Protein Kinase C-lambda
D007527:Isoenzymes; D011493:Protein Kinase C; D000099293:Protein Kinase C-lambda