[Depolymerization of high-molecular-weight chitosan by the enzyme preparation Celloviridine G20x].
A low-molecular-weight water-soluble chitosan was obtained from high-molecular-weight crab chitosan using the enzyme preparation Celloviridine G20x. Optimum conditions for the enzymatic hydrolysis were designed. The reaction should be performed for 4 h in a sodium-acetate buffer (pH 5.2) at 55 degrees C and the enzyme to substrate ratio of 1:400. Fractional extraction of chitosan hydrolysate by aqueous ethanol (ethanol: distilled water) yielded fractions with molecular weights in the range 3.2-26.4 kDa.
Il'ina
A V
AV
Bioinzheneriya Center, Russian Academy of Sciences, Moscow, 117312 Russia.
Tkacheva
Iu V
IuV
Varlamov
V P
VP
D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D003386:Brachyura; D002480:Cellulase / Q000737:chemistry*; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000302:isolation & purification; D048271:Chitosan; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases; D008970:Molecular Weight; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry*
D001704:Biopolymers; D002686:Chitin; D048271:Chitosan; D006867:Hydrolases; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase