Gap junctions in Drosophila: developmental expression of the entire innexin gene family.
Invertebrate gap junctions are composed of proteins called innexins and eight innexin encoding loci have been identified in the now complete genome sequence of Drosophila melanogaster. The intercellular channels formed by these proteins are multimeric and previous studies have shown that, in a heterologous expression system, homo- and hetero-oligomeric channels can form, each combination possessing different gating characteristics. Here we demonstrate that the innexins exhibit complex overlapping expression patterns during oogenesis, embryogenesis, imaginal wing disc development and central nervous system development and show that only certain combinations of innexin oligomerization are possible in vivo. This work forms an essential basis for future studies of innexin interactions in Drosophila and outlines the potential extent of gap-junction involvement in development.
Stebbings
Lucy A
LA
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
Todman
Martin G
MG
Phillips
Rose
R
Greer
Claire E
CE
Tam
Jennifer
J
Phelan
Pauline
P
Jacobs
Kirsten
K
Bacon
Jonathan P
JP
Davies
Jane A
JA
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000196:embryology; D002875:Chromosomes / Q000648:ultrastructure; D017630:Connexins / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000378:metabolism; D029721:Drosophila Proteins / Q000096:biosynthesis*; D004331:Drosophila melanogaster; D015870:Gene Expression; D017403:In Situ Hybridization; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000196:embryology; D017386:Sequence Homology, Amino Acid; D014921:Wings, Animal / Q000196:embryology
D017630:Connexins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D012333:RNA, Messenger