Interleukin-6-producing cells in a human glioblastoma cell line are not affected by ionizing radiation.
We investigated the production of interleukin 6 (IL-6) by a radioresistant human glioblastoma cell line G5 after single radiation events of 3, 6 and 9 Gy. The total cell number and IL-6 concentration in culture supernatant were assessed 24-96 h after irradiation. The radiation impeded or stopped G5 cell growth in a dose-dependent manner, but unexpectedly did not affect the IL-6 concentration in cell culture media that increased in the same range as in non-irradiated cultures. Furthermore, using flow cytometry, we found that the IL-6 positive cells expansion was unaffected by radiation. These findings suggested that this small (about 1%) fraction of G5 cells, constitutively producing IL-6, is highly radioresistant.
Dubost
Jean-Jacques
JJ
Unité d'Immunologie Clinique, Hĵpital Gabriel Montpied, Lyon, France.
Rolhion
Christine
C
Tchirkov
Andrei
A
Bertrand
Suzanne
S
Chassagne
Jacques
J
Dosgilbert
Annie
A
Verrelle
Pierre
P
D001932:Brain Neoplasms; D002452:Cell Count; D002455:Cell Division / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005909:Glioblastoma; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis*; D011839:Radiation, Ionizing; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
D015850:Interleukin-6