title: Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae.
abstract: Simian virus 40 (SV40) utilizes endocytosis through caveolae for infectious entry into host cells. We found that after binding to caveolae, virus particles induced transient breakdown of actin stress fibers. Actin was then recruited to virus-loaded caveolae as actin patches that served as sites for actin "tail" formation. Dynamin II was also transiently recruited. These events depended on the presence of cholesterol and on the activation of tyrosine kinases that phosphorylated proteins in caveolae. They were necessary for formation of caveolae-derived endocytic vesicles and for infection of the cell. Thus, caveolar endocytosis is ligand-triggered and involves extensive rearrangement of the actin cytoskeleton.
authors.affiliation: Swiss Federal Institute of Technology Zurich (ETHZ), HPM1 Building, ETH Hönggerberg, CH-8093 Zurich, Switzerland.
authors.forename: Lucas
authors.initials: L
authors.lastname: Pelkmans
authors.forename: Daniel
authors.initials: D
authors.lastname: Püntener
authors.forename: Ari
authors.initials: A
authors.lastname: Helenius
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D021941:Caveolae / Q000378:metabolism* / Q000648:ultrastructure / Q000821:virology; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000502:physiology; D047630:Depsipeptides; D034281:Dynamins; D004705:Endocytosis; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D000882:Haplorhini; D010456:Peptides, Cyclic / Q000494:pharmacology; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013539:Simian virus 40 / Q000502:physiology*; D022502:Stress Fibers / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D051242:Caveolin 1; D022461:Caveolins; D047630:Depsipeptides; D010456:Peptides, Cyclic; D011993:Recombinant Fusion Proteins; D013844:Thiazoles; D053778:Thiazolidines; C057531:jasplakinolide; D019000:Phosphotyrosine; D002784:Cholesterol; D011505:Protein-Tyrosine Kinases; D020558:GTP Phosphohydrolases; D034281:Dynamins; C037067:latrunculin A