30S ribosomal subunit assembly is a target for inhibition by aminoglycosides in Escherichia coli.
The aminoglycosides paromomycin and neomycin were examined in Escherichia coli cells for an inhibitory effect on 30S ribosomal subunit assembly. Both compounds inhibited the growth rate, viable cell number, and protein synthesis rate with similar 50% inhibitory concentrations. Each drug also showed a concentration-dependent inhibition of 30S subunit formation. The inhibitory effect on 30S particle formation was approximately equivalent to the inhibitory effect on translation for these antibiotics.
Mehta
Roopal
R
Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA.
Champney
W Scott
WS
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D020128:Inhibitory Concentration 50; D009355:Neomycin / Q000494:pharmacology*; D010303:Paromomycin / Q000494:pharmacology*; D012335:RNA, Ribosomal / Q000187:drug effects* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000187:drug effects / Q000378:metabolism
D000900:Anti-Bacterial Agents; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D010303:Paromomycin; D009355:Neomycin