Partial heat denaturation step during reverse transcription and PCR screening yields full-length 5'-cDNAs.
Hüttemann
Maik
M
Center for Molecular Medicine and Genetics, Wayne State University, School of Medicine, Detroit, MI 48201, USA. maik@genetics.wayne.edu
D001483:Base Sequence; D018076:DNA, Complementary / Q000096:biosynthesis*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic
D018076:DNA, Complementary