Differentiation and stromal-induced growth promotion of murine prostatic tumors.
BACKGROUND
We have derived a panel of p53-null prostatic "basal" and "luminal" epithelial cell lines and their ras transformed counterparts to study stromal/epithelial interactions and the properties of tumors arising from "basal" and "luminal" cells.


METHODS
Previously derived normal murine prostatic "basal" epithelial (PE-B-1) and "luminal" epithelial (PE-L-1) cell lines were transformed with N-Ras. These lines and a spontaneously transformed "luminal" cell line were inoculated subcutaneously or orthotopically into athymic mice, alone or in combination with normal prostatic smooth muscle cells (SMC).


RESULTS
All transformed lines formed subcutaneous tumors. SMC significantly enhanced the growth rate of the tumors arising from the "basal" and one of the "luminal" cell lines. The transformed "basal" line gave rise to tumors expressing both "basal" and "luminal" cytokeratins.


CONCLUSIONS
Prostatic SMC promote the growth of transformed epithelial cells, suggesting that prostatic stroma may promote tumor development. Furthermore, transformed "basal" cells give rise to tumors containing "luminal" cells, suggesting that although most human tumors have a "luminal" phenotype, they may originate from transformed "basal" cells.
Salm
Sarah N
SN
Department of Cell Biology, MSB 634, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA. salms01@endeavor.med.nyu.edu
Takao
Tetsuya
T
Tsujimura
Akira
A
Coetzee
Sandra
S
Moscatelli
David
D
Wilson
E Lynette
EL
D000362:Agar; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002461:Cell Line, Transformed; D017690:Cell Transplantation; D003470:Culture Media; D013196:Dihydrotestosterone / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009130:Muscle, Smooth / Q000166:cytology; D009368:Neoplasm Transplantation; D010641:Phenotype; D011467:Prostate / Q000166:cytology / Q000502:physiology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D017154:Stromal Cells / Q000502:physiology* / Q000637:transplantation; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016212:Transforming Growth Factor beta; D013196:Dihydrotestosterone; D000362:Agar