Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis.
The unusual "glycolate" extender unit at C-9/C-10 of ansamitocin is not derived from 2-hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-13C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin bisosynthetic gene cluster.
Carroll
Brian J
BJ
Department of Chemistry, Box 351700, University of Washington, Seattle, Washington 98195-1700, USA.
Moss
Steven J
SJ
Bai
Linquan
L
Kato
Yasuo
Y
Toelzer
Sabine
S
Yu
Tin-Wein
TW
Floss
Heinz G
HG
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D006016:Glycolates / Q000378:metabolism*; D008453:Maytansine / Q000031:analogs & derivatives* / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D005810:Multigene Family
D006016:Glycolates; D009097:Multienzyme Complexes; D008453:Maytansine; C015682:ansamitocins