ik3-2, a relative to ik3-1/cables, is associated with cdk3, cdk5, and c-abl.
A cDNA coding for ik3-2 (designated as ik3-2 from an interactor-2 with cdk3) was cloned by cross-hybridization with ik3-1 and RT-PCR. Analysis of amino acid sequence indicated that ik3-2 has the C-terminal cyclin-box-like region highly homologous to that of ik3-1 (identity in amino acids: 78%). On the other hand, the remainder of ik3-2 gene is not so similar to that of ik3-1. There are several regions other than the C-terminal cyclin-box-like region that are conserved between ik3-1 and ik3-2. In vivo binding assay indicated that like ik3-1, ik3-2 binds to cdk3, cdk5, and c-abl, although ik3-2 binds to cdk3 weakly as compared with ik3-1. The C-terminal cyclin-box-like region of ik3-2 (123 amino acids) is able to be associated with cdk5. Accordingly, ik3-2 is very similar to ik3-1 concerning its molecular interaction with other molecules, suggesting that ik3-2 function in the same biological field as ik3-1. Northern blot analysis indicated that ik3-2 is expressed ubiquitously all over tissues.
Sato
Hiroko
H
Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan.
Nishimoto
Ikuo
I
Matsuoka
Masaaki
M
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D056849:Cyclin-Dependent Kinase 3; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D011485:Protein Binding; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism; D014162:Transfection
C486568:CABLES1 protein, human; C411580:Cables1 protein, mouse; C457759:Cables2 protein, mouse; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D018076:DNA, Complementary; D010750:Phosphoproteins; D016315:Proto-Oncogene Proteins c-abl; D051360:Cyclin-Dependent Kinase 5; C490914:Cdk3 protein, mouse; C495906:Cdk5 protein, mouse; D056849:Cyclin-Dependent Kinase 3; D018844:Cyclin-Dependent Kinases