Casein zymography of calpains using a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-imidazole buffer.
Croall
Dorothy E
DE
Department of Biochemistry, University of Maine, Orono, Maine 04469-5735, USA. croall@maine.edu
Moffett
Katherin
K
Hatch
Harold
H
D000818:Animals; D002021:Buffers; D002154:Calpain / Q000302:isolation & purification*; D002364:Caseins; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006531:HEPES; D007093:Imidazoles; D007526:Isoelectric Point; D007527:Isoenzymes / Q000302:isolation & purification; D051379:Mice; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification
D002021:Buffers; D002364:Caseins; D007093:Imidazoles; D007527:Isoenzymes; D011994:Recombinant Proteins; D002154:Calpain; D006531:HEPES