Attenuation of experimental allergic encephalomyelitis in complement component 6-deficient rats is associated with reduced complement C9 deposition, P-selectin expression, and cellular infiltrate in spinal cords.
The role of Ab deposition and complement activation, especially the membrane attack complex (MAC), in the mediation of injury in experimental allergic encephalomyelitis (EAE) is not resolved. The course of active EAE in normal PVG rats was compared with that in PVG rats deficient in the C6 component of complement (PVG/C6(-)) that are unable to form MAC. Following immunization with myelin basic protein, PVG/C6(-) rats developed significantly milder EAE than PVG/C rats. The anti-myelin basic protein response was similar in both strains, as was deposition of C3 in spinal cord. C9 was detected in PVG/C rats but not in PVG/C6(-), consistent with their lack of C6 and inability to form MAC. In PVG/C6(-) rats, the T cell and macrophage infiltrate in the spinal cord was also significantly less than in normal PVG/C rats. There was also reduced expression of P-selectin on endothelial cells, which may have contributed to the reduced cellular infiltrate by limiting migration from the circulation. Assay of cytokine mRNA by RT-PCR in the spinal cords showed no differences in the profile of Th1 or Th2 cytokines between PVG/C and PVG/C6(-) rats. PVG/C rats also had a greater increase in peripheral blood white blood cell, neutrophil, and basophil counts than was observed in the PVG/C6(-). These findings suggest that the MAC may have a role in the pathogenesis of EAE, not only by Ig-activated MAC injury but also via induction of P-selectin on vascular endothelium to promote infiltration of T cells and macrophages into the spinal cord.
Tran
Giang T
GT
Department of Medicine, University of New South Wales, Liverpool Hospital, Liverpool, New South Wales, Australia.
Hodgkinson
Suzanne J
SJ
Carter
Nicole
N
Killingsworth
Murray
M
Spicer
S Timothy
ST
Hall
Bruce M
BM
D000818:Animals; D002465:Cell Movement; D003183:Complement C6 / Q000235:genetics*; D003186:Complement C9 / Q000378:metabolism*; D015938:Complement Membrane Attack Complex / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D007136:Immunoglobulins / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007700:Kinetics; D007958:Leukocyte Count; D004676:Myelin Basic Protein / Q000276:immunology; D019007:P-Selectin / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology*
D003183:Complement C6; D003186:Complement C9; D015938:Complement Membrane Attack Complex; D016207:Cytokines; D007136:Immunoglobulins; D004676:Myelin Basic Protein; D019007:P-Selectin; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1