The Sambucus nigra type-2 ribosome-inactivating protein SNA-I' exhibits in planta antiviral activity in transgenic tobacco.
Transgenic tobacco (Samsun NN) plants transformed with a cDNA clone encoding SNA-I' from Sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. Expression of SNA-I' under the control of the 35S cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. In contrast to type-1 ribosome-inactivating proteins, the expression of SNA-I' does not affect the growth and fertility of the transgenic plants and is not accompanied by an increased expression of pathogenesis-related proteins indicating that its antiviral activity most probably differs from that of pokeweed antiviral protein.
Chen
Ying
Y
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001, Leuven, Belgium.
Peumans
Willy J
WJ
Van Damme
Els J M
EJ
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D027924:Sambucus nigra / Q000235:genetics; D014026:Nicotiana / Q000187:drug effects* / Q000821:virology*; D014027:Tobacco Mosaic Virus
D000998:Antiviral Agents; D010940:Plant Proteins; D011994:Recombinant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D009699:N-Glycosyl Hydrolases; C024275:pokeweed antiviral protein