Agrobacterium tumefaciens supports DNA replication of diverse geminivirus types.
We have previously shown that the soil-borne plant pathogen Agrobacterium tumefaciens supports the replication of tomato leaf curl geminivirus (Australian isolate) (TLCV) DNA. However, the reproducibility of this observation with other geminiviruses has been questioned. Here, we show that replicative DNA forms of three other geminiviruses also accumulate at varying levels in Agrobacterium. Geminiviral DNA constructs that lacked the ability to replicate in Agrobacterium were rendered replication-competent by changing their configuration so that two copies of the viral ori were present. Furthermore, we report that low-level replication of TLCV DNA can occur in Escherichia coli containing a dimeric TLCV construct in a high copy number plasmid. These findings were reinforced by expression studies using beta-glucuronidase which revealed that all six TLCV promoters are active in Agrobacterium, and two are functional in E. coli.
Selth
Luke A
LA
CSIRO Plant Industry, Horticulture Unit, PO Box 350, Glen Osmond, SA, Australia.
Randles
John W
JW
Rezaian
M Ali
MA
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000821:virology*; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D017791:Geminiviridae / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D017930:Genes, Reporter; D005814:Genes, Viral; D005966:Glucuronidase / Q000235:genetics; D010944:Plants / Q000821:virology; D011401:Promoter Regions, Genetic; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral; D005966:Glucuronidase