Antimicrobial resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization.
Fourteen and 22 each of Salmonella Enteritidis and Salmonella Typhimurium (S. Typhimurium) were isolated from animals from 1983 to 1999 in Korea and tested for their antibiotic resistance patterns, phage types and resistance gene patterns. S. Typhimurium isolates were highly resistant to streptomycin, sulfisoxazole and tetracycline, 95, 95 and 86%, respectively. The incidence of multiple antibiotic resistance (resistant to more than two drugs tested) of S. Typhimurium isolates was extremely high (100%) comparing to S. Enteritidis isolates (21%). Two of the five ACSSuT (ampicillin, chloramphenicol, streptomycin, sulfisoxazole and tetracycline) resistant type S. Typhimurium isolates were phage type definitive type 104 (DT104). For the detection of resistance related genes in S. Enteritidis and S. Typhimurium isolates, particularly ACSSuT type S. Typhimurium, antibiotic resistance genes, cmlA/tetR, bla(PSE-1) and bla(TEM), and genus Salmonella specific gene, sipB/C, were amplified using four pairs of primers in a hot-start multiplex polymerase chain reaction (PCR). Two Korean isolates of S. Typhimurium DT104 showed bla(TEM) amplicons instead of bla(PSE-1) for the ampicillin resistance and they were susceptible to florfenicol. The multiplex PCR used in this study was useful in characterization of multiple drug resistant Salmonella isolates, especially ACSSuT type S. Typhimurium, and identification of beta-lactamase gene distribution among Salmonella isolates.
Yang
Soo Jin
SJ
Department of Veterinary Microbiology and Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, 441-744, Suwon, South Korea.
Park
Kyoung Yoon
KY
Kim
So Hyun
SH
No
Kyoung Min
KM
Besser
Thomas E
TE
Yoo
Han Sang
HS
Kim
Shuk Ho
SH
Lee
Bok Kwon
BK
Park
Yong Ho
YH
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001434:Bacteriophage Typing; D001483:Base Sequence; D024881:Drug Resistance, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D005784:Gene Amplification; D005838:Genotype; D007723:Korea; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000188:drug therapy / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D012486:Salmonella typhimurium / Q000145:classification / Q000187:drug effects* / Q000235:genetics
D000900:Anti-Bacterial Agents