title: Low frequency noise and whole-body vibration cause increased levels of sister chromatid exchange in splenocytes of exposed mice.
abstract: Chronic exposure to low frequency (LF) noise and whole-body vibration (WBV) induces both physiological and psychological alterations in man. Recently, we have shown that long-term occupational exposure to LF noise and WBV produces genotoxic effects in man expressed as an increase in sister chromatid exchange (SCE) levels in lymphocytes. The objectives of the present study were to investigate whether the observed effect could be reproduced in a murine model and, if so, which of the agents, LF noise alone or in combination with WBV, would be instrumental in the SCE induction. SCEs were analyzed in spleen lymphocytes of mice exposed to LF noise alone and in combination with WBV for 300 and 600 hr. An effect at the cell cycle kinetics level was also investigated. The results revealed significant increases in the mean SCE number per cell and in the proportion of cells with high frequency of SCEs (HFCs) in lymphocytes of mice submitted to combined noise and WBV over controls. No significant differences were found between single noise-exposed and control mice. A cell cycle delay was observed exclusively in the noise and WBV exposure groups. In conclusion, we demonstrated that, as in exposed workers, prolonged exposure to the combination of LF noise and WBV determines an increase in SCE level in mice while LF noise alone is not effective in SCE induction.
authors.affiliation: Center of Human Genetics, National Institute of Health, Lisbon, Portugal. m.joao.silva@insa.min-saude.pt
authors.forename: M J
authors.initials: MJ
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Dias
authors.forename: A
authors.initials: A
authors.lastname: Barreta
authors.forename: P J
authors.initials: PJ
authors.lastname: Nogueira
authors.forename: N A A
authors.initials: NA
authors.lastname: Castelo-Branco
authors.forename: M G
authors.initials: MG
authors.lastname: Boavida
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002478:Cells, Cultured; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009622:Noise / Q000009:adverse effects*; D012854:Sister Chromatid Exchange; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D014732:Vibration