title: hNT neurons express an immunosuppressive protein that blocks T-lymphocyte proliferation and interleukin-2 production.
abstract: Ntera2/D1 cells had an A1 B8 Bw6 Cw7 DR3 DR52 major histocompatibility complex (MHC) genotype. Its neuronal derivative, hNT neurons, expressed A1 B8 Bw6 MHC class I molecules, but did not activate, and its hNT supernatant suppressed allogeneic mixed lymphocyte cultures (MLC) >98% (p<0.01), phytohemagglutinin (PHA)-activated T-cell proliferation >87% (p<0.01), even 48 h after stimulation, suppressed phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced T-cell proliferation >99% (p<0.001), and reduced interleukin-2 (IL-2) production (p<0.01), while maintaining T cells in a quiescent G(0)/G(1) state without lowering their viability. This immunosuppressive activity was attributed to a 40-100-kDa anionic hNT protein with an isoelectric point of 4.8.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
authors.forename: W R
authors.initials: WR
authors.lastname: Gower
authors.forename: P R
authors.initials: PR
authors.lastname: Sanberg
authors.forename: P G
authors.initials: PG
authors.lastname: Brown
authors.forename: S
authors.initials: S
authors.lastname: Salyani
authors.forename: C J
authors.initials: CJ
authors.lastname: Pasqual
authors.forename: M
authors.initials: M
authors.lastname: McGrogan
authors.forename: R A
authors.initials: RA
authors.lastname: Good
authors.forename: R W
authors.initials: RW
authors.lastname: Engelman
mesh_terms: D016380:Brain Tissue Transplantation / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D017077:Culture Media, Conditioned; D015870:Gene Expression / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation / Q000276:immunology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000276:immunology; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2