Incorporation of fatty acids into the outer and inner membranes of isolated rat liver mitochondria.
Acyl-CoA: phospholipid acyl-transferase activity as well as phospholipase A activity were detected in inner and outer membrane preparations from rat liver mitochondria. Both enzyme systems have an optimum pH around 8 and act preferentially on phosphatidylethanolamine. While phospholipase A activity is much lower in the inner membrane than in the outer membrane of mitochondria the reverse is true for the incorporation of (14C)-oleic acid into endogenous phosphatidylethanolamine. These results bring an indirect evidence that the inner membrane per se possesses a phospholipase A activity.
Nachbaur
J
J
Biochimie, C.E.N.-G. et Faculté de Médecine, Cédex 85, 38 -, Grenoble-Gare, France
Colbeau
A
A
Vignais
P M.
PM