Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens.
An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydans and A. liquefaciens. Although both lac genes were functionally expressed in both acetic acid bacteria, only a few transformants were able to grow on lactose. However, this ability strictly depended on the presence of a plasmid expressing both lac genes. Mutations in the plasmids and/or in the chromosome were excluded as the cause of growth ability on lactose.
Mostafa
Hesham E
HE
Institute for Microbiology, Federal Dairy Research Centre, 24103 Kiel, Germany.
Heller
Knut J
KJ
Geis
Arnold
A
D000091:Acetobacter / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005822:Genetic Vectors; D020600:Gluconobacter oxydans / Q000235:genetics / Q000254:growth & development; D007763:Lac Operon / Q000235:genetics*; D007785:Lactose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D009004:Monosaccharide Transport Proteins; D010957:Plasmids / Q000235:genetics; D027981:Symporters
D003470:Culture Media; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D007785:Lactose