Cytotoxic dammarane glycosides from processed ginseng.
Steaming ginseng at high temperature increased its cytotoxicity to SK-Hep-1 hepatoma cancer cells. HPLC separation and fractionation followed by MTT assay revealed that ginsenosides Rg3, Rg5, Rk1, Rs5, and Rs4 are the active principles. Their 50% growth inhibition concentration (GI50) values were 41, 11, 13, 37, and 13 microM, respectively. Cisplatin had a GI50 of 84 microM in the same assay conditions.
Park
Il Ho
IH
College of Pharmacy, Seoul National University, South Korea.
Piao
Long Zhu
LZ
Kwon
Sung Won
SW
Lee
Yong Jae
YJ
Cho
Sool Yeon
SY
Park
Man Ki
MK
Park
Jeong Hill
JH
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000494:pharmacology; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008113:Liver Neoplasms / Q000473:pathology; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013696:Temperature; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000097669:Dammaranes
D000972:Antineoplastic Agents, Phytogenic; D002945:Cisplatin; D036145:Ginsenosides; D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes; D000097669:Dammaranes