Molecular differentiation between NS1 gene of a field strain Bluetongue virus serotype 2 (BTV-2) and NS1 gene of an attenuated BTV-2 vaccine.
At the end of September 2000, clinical symptoms of Bluetongue appeared in sheep flocks of the Balearic Islands (Spain). The presence of the BTV serotype 2 in tissue and blood samples of affected animals was confirmed by laboratory techniques. A systematic vaccination were carried out in affected areas using a live monovalent serotype 2 vaccine available from Onderstepoort laboratory (South Africa). In order to perform epidemiological studies, a new method to differentiate between the NS1 genes of BTV-2 affecting the Balearic islands and that of the Onderstepoort commercial live virus vaccine (monovalent, serotype 2) has been developed. This procedure is based on the use of an RT-PCR, followed by restriction endonuclease analysis. Epidemiological data of a study carried out in the period January-October 2001 using this procedure are included.
Agüero
M
M
Centro de Investigación en Sanidad Animal (CISA-INIA), CISA, 28130, Voldeolmos, Madrid, Spain.
Arias
M
M
Romero
L J
LJ
Zamora
M J
MJ
Sánchez-Vizcaíno
J M
JM
D000818:Animals; D001819:Bluetongue / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control; D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D012703:Serotyping; D012756:Sheep; D013019:South Africa; D013030:Spain / Q000453:epidemiology; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*
D005819:Genetic Markers; C078966:NS1 protein, bluetongue virus; D014613:Vaccines, Attenuated; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes