title: A tomato cysteine protease required for Cf-2-dependent disease resistance and suppression of autonecrosis.
abstract: Little is known of how plant disease resistance (R) proteins recognize pathogens and activate plant defenses. Rcr3 is specifically required for the function of Cf-2, a Lycopersicon pimpinellifolium gene bred into cultivated tomato (Lycopersicon esculentum) for resistance to Cladosporium fulvum. Rcr3 encodes a secreted papain-like cysteine endoprotease. Genetic analysis shows Rcr3 is allelic to the L. pimpinellifolium Ne gene, which suppresses the Cf-2-dependent autonecrosis conditioned by its L. esculentum allele, ne (necrosis). Rcr3 alleles from these two species encode proteins that differ by only seven amino acids. Possible roles of Rcr3 in Cf-2-dependent defense and autonecrosis are discussed.
authors.affiliation: The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK.
authors.forename: Julia
authors.initials: J
authors.lastname: Krüger
authors.forename: Colwyn M
authors.initials: CM
authors.lastname: Thomas
authors.forename: Catherine
authors.initials: C
authors.lastname: Golstein
authors.forename: Mark S
authors.initials: MS
authors.lastname: Dixon
authors.forename: Matthew
authors.initials: M
authors.lastname: Smoker
authors.forename: Saijun
authors.initials: S
authors.lastname: Tang
authors.forename: Lonneke
authors.initials: L
authors.lastname: Mulder
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002963:Cladosporium / Q000502:physiology*; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics; D019076:Transgenes
chemical_list: D015853:Cysteine Proteinase Inhibitors; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D003546:Cysteine Endopeptidases; D007930:Leucine; C024974:E 64