[Enzymatic synthesis of monoglycerides in microaqueous media by using lipase from Pseudomonus fluorescens].
Enzymatic synthesis of monoglycerides by glycerolysis of oil and fats in microaqueous solvent-free media was investigated by using lipase from pseudomonus fluorescens (PFL). Initial eutectic point(IEP) was substituted for melt point of oil and fats in Critical Temperature Theory. By investigating the glycerolysis under different IEP, it is showed that there is a relationship between composition of the oils and the yield of monoglycerides: Y = -0.0006 X3 + 0.0592 X2 - 0.8909 X + 26.753(13% < X < 76.5%), here X is the contents(W/W) of saturated fatty acid residue (C16 + C18) in the oils, Y is the yield of monoglycerides at 40 degrees C. The optimum isothermal reaction conditions for a system which IEP is 40 degrees C are: 40 degrees C, 3%-4.5% (W/W) water in glycerol, dosage of lipase is 500 u/g oil when the mole ratio of glycerol to oil is 2.5:1. The highest yield of monoglycerides is 81.4% in 48 h by means of programming temperature reaction.
Xia
Yong-Mei
YM
Southern Yangzi University, School of Chemical Engineering, Wuxi 214036, China. yongmeix@163.net
Zhang
Ke-Chang
KC
Shi
Gui-Yang
GY
Li
Xiao-Hui
XH
Fang
Yun
Y
D005989:Glycerides / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D005989:Glycerides; D010938:Plant Oils; D000073878:Palm Oil; D008049:Lipase; D005990:Glycerol