Overexpression of the cytotoxic T cell GalNAc transferase in skeletal muscle inhibits muscular dystrophy in mdx mice.
Duchenne muscular dystrophy (DMD) is a congenital X-linked myopathy caused by lack of dystrophin protein expression. In DMD, the expression of many dystrophin-associated proteins (DAPs) is reduced along the sarcolemmal membrane, but the same proteins remain concentrated at the neuromuscular junction where utrophin, a dystrophin homologue, is expressed [Matsumura, K., Ervasti, J. M., Ohlendieck, K., Kahl, K. D. & Campbell, K. (1992) Nature (London) 360, 588-591]. This outcome has led to the concept that ectopic expression of a "synaptic scaffold" of DAPs and utrophin along myofibers might compensate for the molecular defects in DMD. Here we show that transgenic overexpression of the synaptic CT GalNAc transferase in the skeletal muscles of mdx animals (mdx/CT) increases the expression of utrophin and many DAPs, including dystroglycans, sarcoglycans, and dystrobrevins, along myofibers. Protein expression of utrophin and DAPs was equal to or above that of wild-type mice. In addition, alpha-dystroglycan was glycosylated with the CT carbohydrate antigen in mdx/CT but not in mdx muscles. mdx/CT mice have little or no evidence of muscular dystrophy by several standard measures; Serum creatine kinase levels, percentage of centrally located myofiber nuclei, and variance in myofiber diameter in mdx/CT muscles were dramatically reduced compared with mdx mice. These data suggest that ectopic expression of the CT GalNAc transferase creates a functional dystrophin-related complex along myofibers in the absence of dystrophin and should be considered as a target for therapeutic intervention in DMD.
Nguyen
Holly H
HH
Department of Neuroscience, Glycobiology Research and Training Center, University of California at San Diego School of Medicine, La Jolla, CA 92093-0691, USA.
Jayasinha
Vianney
V
Xia
Bing
B
Hoyte
Kwame
K
Martin
Paul T
PT
D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D003598:Cytoskeletal Proteins / Q000378:metabolism; D049030:Dystroglycans; D016189:Dystrophin / Q000378:metabolism; D049029:Dystrophin-Associated Proteins; D006031:Glycosylation; D015151:Immunoblotting; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D018101:Mice, Inbred mdx; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000201:enzymology*; D009136:Muscular Dystrophies / Q000201:enzymology; D017350:N-Acetylgalactosaminyltransferases / Q000096:biosynthesis*; D009479:Neuropeptides / Q000378:metabolism; D013601:T-Lymphocytes / Q000201:enzymology*; D013997:Time Factors; D019076:Transgenes; D049411:Utrophin
D003598:Cytoskeletal Proteins; D016189:Dystrophin; D049029:Dystrophin-Associated Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009479:Neuropeptides; C483600:Utrn protein, mouse; D049411:Utrophin; C098885:dystrobrevin; D049030:Dystroglycans; D017350:N-Acetylgalactosaminyltransferases; D003402:Creatine Kinase