[Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line].
The aim of the present study was to investigate whether interleukin-2 (IL-2) is involved in the proliferation control of the cultured RC-4B/C cell, which is a derived pituitary adenoma cell line of the rat. The level of cell proliferation was estimated by assessing (3)H-thymidine ((3)H-TdR) incorporation rate. IL-2 (10 1000 U/ml) significantly stimulated (3)H-TdR incorporation into the cell line in a dose-dependent manner. Specific PTK inhibitor tyrphostin (1 micromol/L) suppressed RC-4B/C cell proliferation and blocked the effect of IL-2 on RC-4B/C cells. After the PKA signaling pathway was inhibited by specific PKA inhibitor H-9 (1 micromol/L), the proliferation rate of RC-4B/C cells increased significantly. H-9 also enhanced the stimulation of IL-2 on RC-4B/C cell growth. Anti-estrogen tamoxifen (1 micromol/L) had no significant effect on the action of IL-2 on the proliferation of RC-4B/C cells. In conclusion, it is suggested that IL-2 modulates the proliferation of the cultured RC-4B/C pituitary adenoma cell line, and the action is closely related with the PTK and PKA signaling pathway.
Wang
G F
GF
The Fourth Military Medical University, Xi an 710032, China. wanggf@fmmu.edu.cn
Zhu
Y L
YL
Chen
J K
JK
Zhang
W H
WH
Zhong
Y Q
YQ
Hu
Y Z
YZ
Wang
F Z
FZ
D000236:Adenoma / Q000473:pathology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D010911:Pituitary Neoplasms / Q000473:pathology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D015398:Signal Transduction; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D020032:Tyrphostins / Q000494:pharmacology
D007376:Interleukin-2; D020032:Tyrphostins; D013629:Tamoxifen; D011505:Protein-Tyrosine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases