title: Do Ki67, S-phase, S + G2M and DNA ploidy, evaluated by flow cytometry, reveal locoregional metastasis in oral cavity and oropharynx carcinomas?
abstract: Tumour cell proliferation is an important biological prognostic parameter to be considered alongside clinical and histopathological parameters. It has been evaluated by immunohistochemistry using proliferative markers (PCNA, Ki67, etc.) and by flow cytometry considering DNA content, growth fraction (S + G2M) and S-phase fraction. Our aim was to evaluate by flow cytometry both Ki67 and S-phase fraction, S + G2M, DNA content in patients surgically treated for oral cavity and/or oropharynx carcinomas. The study, performed on archival material, analysed the significance and prognostic reliability of these biological parameters to reveal locoregional metastasis and evaluated their possible correlation with clinical and histopathological parameters. In conclusion, tumour cell proliferation seems not to be useful in revealing the presence of locoregional metastasis in oral cavity and oropharynx carcinomas. Thus, new approaches are required.
authors.affiliation: Department of Otorhinolaryngology, Cattinara Hospital, I-34149 Trieste, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Tirelli
authors.forename: Laura
authors.initials: L
authors.lastname: Sidari
authors.forename: Vittorio
authors.initials: V
authors.lastname: Giacomarra
authors.forename: Ledia
authors.initials: L
authors.lastname: Papanikolla
authors.forename: Franco
authors.initials: F
authors.lastname: Sasso
authors.forename: Mario
authors.initials: M
authors.lastname: Russolo
authors.forename: Mauro
authors.initials: M
authors.lastname: Melato
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D005260:Female; D005434:Flow Cytometry; D016195:G2 Phase; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D009062:Mouth Neoplasms / Q000473:pathology*; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D009959:Oropharyngeal Neoplasms / Q000473:pathology*; D011003:Ploidies; D016196:S Phase
chemical_list: D019394:Ki-67 Antigen