[Membrane potential before and after deep freezing of Escherichia coli in the presence of dimethyl sulfoxide and diethyl sulfoxide].
It was shown by the method of penetrating tetraphenylphosphonium cations that low-temperature freezing (-196 degrees C) of Escherichia coli leads to a sharp decrease (from 198 to 85 mV) in membrane potential. Incubation of bacteria in a medium containing dimethyl sulfoxide and diethyl sulfoxide as cryoprotectors results in a reduction of the potential by 16 and 27 mV, respectively. It was also shown that diethyl sulfoxide is more effective in maintaining the membrane potential after freezing--thawing than dimethyl sulfoxide.
Markarian
Sh A
ShA
Department of Chemistry, Yerevan State University, Yerevan, 375049 Armenia.
Bagramian
K A
KA
Arakelian
V B
VB
D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004926:Escherichia coli / Q000502:physiology*; D005615:Freezing; D008564:Membrane Potentials; D013454:Sulfoxides / Q000494:pharmacology*
D003451:Cryoprotective Agents; D013454:Sulfoxides; C454662:diethyl sulfoxide; D004121:Dimethyl Sulfoxide