Parallel synthesis of PNA-peptide conjugate libraries.
An optimized semi-automatic protocol for parallel synthesis of up to 96 peptide nucleic acids (PNA) or PNA-peptide conjugates using Boc-protection strategy has been developed using a robotic system. The approach is illustrated by synthesizing PNA and PNA-peptide libraries varying between 15 and 27  amino acid  units. The peptides (NLS (nuclear localization signal) or Tat-peptide) were attached to N-terminus of the PNA. The method was found to be far superior to that based on the SPOT/Fmoc protocol by which PNA oligomers are synthesized on a modified cellulose membrane. On a 0.5 micromole scale the method typically yielded 2 mg product of 90% purity by HPLC/MALDI-TOF analysis. This approach is suitable for screening of a large number of PNA and/or peptide sequences for biochemical and biological studies.
Awasthi
Satish Kumar
SK
Center for Biomolecular Recognition, IMBG, Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, Copenhagen N, DK 2200, Denmark.
Nielsen
Peter E
PE
D000595:Amino Acid Sequence; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D019151:Peptide Library; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D010880:Piperidines / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics
D007202:Indicators and Reagents; D008567:Membranes, Artificial; D019151:Peptide Library; D020135:Peptide Nucleic Acids; D010880:Piperidines; D001618:beta-Lactamases