Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation.
Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to "fingerprint" analysis by high-resolution 2-D gel electrophoresis. Forty-five spots from 11 x 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.
Vinzant
T B
TB
Biotechnology Center for Fuels and Chemicals, National Renewable Energy Laboratory, Golden, CO 80401, USA.
Adney
W S
WS
Decker
S R
SR
Baker
J O
JO
Kinter
M T
MT
Sherman
N E
NE
Fox
J W
JW
Himmel
M E
ME
D001709:Biotechnology; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D000431:Ethanol / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013058:Mass Spectrometry; D010449:Peptide Mapping; D020543:Proteome; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D020543:Proteome; D000431:Ethanol; D006026:Glycoside Hydrolases; D002480:Cellulase