Analysis of the heat shock response of Neisseria meningitidis with cDNA- and oligonucleotide-based DNA microarrays.
Oligonucleotide- and cDNA-based microarrays comprising a subset of Neisseria meningitidis genes were assessed for study of the meningococcal heat shock response and found to be highly suitable for transcriptional profiling of N. meningitidis. Employing oligonucleotide arrays encompassing the entire genome of N. meningitidis, we analyzed the meningococcal heat shock response on a global scale and identified 55 heat shock-deregulated open reading frames (34 induced and 21 repressed).
Guckenberger
Matthias
M
Institute for Hygiene and Microbiology, University of Würzburg, Germany.
Kurz
Sebastian
S
Aepinus
Christian
C
Theiss
Stephanie
S
Haller
Sabine
S
Leimbach
Thomas
T
Panzner
Ursula
U
Weber
Jacqueline
J
Paul
Hubert
H
Unkmeir
Alexandra
A
Frosch
Matthias
M
Dietrich
Guido
G
D004269:DNA, Bacterial / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018869:Heat-Shock Response; D009345:Neisseria meningitidis / Q000235:genetics / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000235:genetics; D016366:Open Reading Frames
D004269:DNA, Bacterial; D018076:DNA, Complementary; D009841:Oligonucleotides