title: Detection of quantitative trait loci for carcass traits in the pig by using AFLP.
abstract: For evaluation of the suitability of Amplified Fragment Length Polymorphism (AFLP) for detection of quantitative trait loci in farm animals, a combination of AFLP and selective genotyping has been applied as a rapid screening method for marker-QTL associations. Focusing on loci affecting eye muscle area, six extreme discordant sib pairs were selected from a Duroc x Berlin Miniature Pig F2 experimental cross and examined by using 48 AFLP primer combinations. Two prominent AFLP markers were converted into simple codominant PCR markers ( STS-Bo1 and STS-Bo3) and assigned to Sscr4 by physical and linkage mapping. Single marker analysis indicated association of the STS markers with a putative QTL influencing eye muscle area. Interval mapping confirmed the presence of a significant QTL for eye muscle area (Pgenomewide < 0.01) on the Sscr4, with STS-Bo1 being the closer marker. At the same location, significant effects (Pgenomewide < 0.01) on carcass length and backfat thickness were also detected. Our results demonstrate the capability of the combination of AFLP analysis and selective genotyping as a method for detection of genome regions containing QTL in livestock.
authors.affiliation: Institute of Animal Breeding Science, University of Bonn, 53115 Bonn, Germany. wimmers@itz.uni-bonn.de
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.forename: Eduard
authors.initials: E
authors.lastname: Murani
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
mesh_terms: D000818:Animals; D001824:Body Constitution; D002874:Chromosome Mapping; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019655:Quantitative Trait, Heritable; D013552:Swine / Q000235:genetics*
chemical_list: D005819:Genetic Markers