title: Toxicological studies on Polyporus pinsitus laccase expressed by Aspergillus oryzae intended for use in food.
abstract: The laccase used in the study was produced by submerged fermentation of Aspergillus oryzae, containing a gene originating from Polyporus pinsitus. Laccase is to be employed as a processing aid in the juice industry to make a clear and stable juice. The enzyme was subject to a series of toxicological tests to document its safety in use. It was not mutagenic in the Salmonella typhimurium reverse mutation assay, and it did not cause chromosomal aberrations in cultured human lymphocytes. No evidence of inhalation toxicity or skin and eye irritation was found. Oral administration to rat of up to 10 ml kg(-1) b.w. day(-1) (equivalent to a total organic solids dosage of 676 mg kg(-1) b.w. day(-1) or a laccase dosage of 2601 LACU kg(-1) b.w. day(-1)) for 13 weeks did not cause any adverse effect. The maximum recommended dosage of laccase used for juice applications is 50 LACU l(-1) juice.
authors.affiliation: Novozymes A/S, Bagsvaerd, Denmark. dsbr@novo-zymes.com
authors.forename: D S
authors.initials: DS
authors.lastname: Brinch
authors.forename: P B
authors.initials: PB
authors.lastname: Pedersen
mesh_terms: D000280:Administration, Inhalation; D000284:Administration, Oral; D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology*; D001487:Basidiomycota / Q000201:enzymology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003257:Consumer Product Safety; D004334:Drug Administration Schedule; D005123:Eye / Q000187:drug effects; D005260:Female; D005511:Food Handling; D042845:Laccase; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D010088:Oxidoreductases / Q000008:administration & dosage / Q000633:toxicity*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects
chemical_list: D010088:Oxidoreductases; D042845:Laccase