title: The plant Rad50-Mre11 protein complex.
abstract: The Rad50-Mre11-Xrs2/Nbs1 protein complex plays critical roles in cellular processes involving DNA ends. This complex is implicated in DNA recombination and replication, meiosis, telomere maintenance and cellular DNA damage responses. The Rad50 and Mre11 proteins are essential for viability in animals, although not in yeast. We have prepared antibodies to the Rad50 protein of the model plant Arabidopsis thaliana which recognize a 175 kDa protein in wild-type Arabidopsis protein extracts. Furthermore, we report here demonstration of the existence of the Rad50-Mre11 complex by co-immunoprecipitation of the Rad50 and Mre11 proteins from the plant cell extracts.
authors.affiliation: CNRS UMR6547-BIOMOVE, Université Blaise Pascal, 24, Avenue des Landais, 63177 Cedex, Aubiere, France.
authors.forename: S
authors.initials: S
authors.lastname: Daoudal-Cotterell
authors.forename: M E
authors.initials: ME
authors.lastname: Gallego
authors.forename: C I
authors.initials: CI
authors.lastname: White
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D046911:Macromolecular Substances; D009154:Mutation; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011233:Precipitin Tests; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D029681:Arabidopsis Proteins; D046911:Macromolecular Substances; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; C427727:rad50 protein, Arabidopsis; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C094191:MRE11 protein, S cerevisiae