Detection of polyol accumulation in a new ovarian carcinoma cell line, CABA I: a(1)H NMR study.
Ovarian carcinomas represent a major form of gynaecological malignancies, whose treatment consists mainly of surgery and chemotherapy. Besides the difficulty of prognosis, therapy of ovarian carcinomas has reached scarce improvement, as a consequence of lack of efficacy and development of drug-resistance. The need of different biochemical and functional parameters has grown, in order to obtain a larger view on processes of biological and clinical significance. In this paper we report novel metabolic features detected in a series of different human ovary carcinoma lines, by (1)H NMR spectroscopy of intact cells and their extracts. Most importantly, a new ovarian adenocarcinoma line CABA I, showed strong signals in the spectral region between 3.5 and 4.0 p.p.m., assigned for the first time to the polyol sorbitol (39+/-11 nmol/10(6) cells). (13)C NMR analyses of these cells incubated with [1-(13)C]-D-glucose demonstrated labelled-sorbitol formation. The other ovarian carcinoma cell lines (OVCAR-3, IGROV 1, SK-OV-3 and OVCA432), showed, in the same spectral region, intense resonances from other metabolites: glutathione (up to 30 nmol/10(6) cells) and myo-inositol (up to 50 nmol/10(6) cells). Biochemical and biological functions are suggested for these compounds in human ovarian carcinoma cells, especially in relation to their possible role in cell detoxification mechanisms during tumour progression.
Ferretti
A
A
Laboratory of Cell Biology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
D'Ascenzo
S
S
Knijn
A
A
Iorio
E
E
Dolo
V
V
Pavan
A
A
Podo
F
F
D000230:Adenocarcinoma / Q000473:pathology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D018450:Disease Progression; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006859:Hydrogen; D007202:Indicators and Reagents / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy; D010051:Ovarian Neoplasms / Q000473:pathology*; D013012:Sorbitol / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D007202:Indicators and Reagents; D013012:Sorbitol; D006859:Hydrogen; D005978:Glutathione