Paraoxonase1-192 polymorphism modulates the effects of regular and acute exercise on paraoxonase1 activity.
Regular exercise practise is a protective factor against coronary heart disease and enhances antioxidant systems, whereas acute exercise appears to be a major source of increased oxidative stress. Paraoxonase1 (PON1) is an antioxidant HDL-linked enzyme, whose activity toward paraoxon (PON1 activity) is strongly modulated by the PON1-192 polymorphism, comprising Q and R alleles for low and high PON1 activity, respectively. Another polymorphism at the PON1 locus, the PON1-55, modulates PON1 protein and activity levels. PON1 activity, lipid levels, and oxidized LDL concentration were determined in 17 healthy young volunteers before and after a 16-weeks aerobic exercise training period. Furthermore, PON1 activity was analyzed after a bout of exercise in both situations. We found that regular exercise was associated with a decrease in oxidized LDL levels, and an increase in PON1 activity in QQ subjects and with a decrease in PON1 activity in R carriers. A bout of exercise produced an increase in PON1 activity just after the bout of exercise, followed by a decrease in its activity. A recovery of the basal PON1 activity levels at 24 h was found in QQ subjects regardless of their training status and in trained R carriers, but not in untrained R carriers. These results suggest that the effects of regular and acute exercise on PON1 activity levels are modulated by PON1-192 polymorphism. Changes were less evident for the PON1-55 polymorphism.
Tomás
Marta
M
Lipids and Cardiovascular Epidemiology Unit, Departament de Ciències Experimentals i de la Salut, Institut Municipal d'Investigació Mèdica (IMIM), Dr Aiguader 80, E-08003 Barcelona, Spain. mtomas@imim.es
Elosua
Roberto
R
Sentí
Mariano
M
Molina
Luis
L
Vila
Joan
J
Anglada
Roger
R
Fitó
Montserrat
M
Covas
Maria Isabel
MI
Marrugat
Jaume
J
D000328:Adult; D043303:Aryldialkylphosphatase; D001827:Body Height; D015992:Body Mass Index; D001835:Body Weight; D016009:Chi-Square Distribution; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003327:Coronary Disease / Q000517:prevention & control; D004950:Esterases / Q000235:genetics* / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005260:Female; D006580:Genetic Carrier Screening; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D010807:Physical Endurance / Q000502:physiology; D010809:Physical Fitness; D012016:Reference Values; D012907:Smoking; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D007527:Isoenzymes; D008077:Lipoproteins, LDL; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D002784:Cholesterol; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human