[Nucleotide sequence and protein sequence analysis of GL-7-ACA acylase from Pseudomonas sp. 130].
The nucleotide sequence and N-, C-terminal amino acid sequences of alpha,beta-subunit of glutaryl 7-ACA acylase C130 from Pseudomonas sp. 130 were determined. The alignment of the acylase C130 with the other acylases shows that it has high homology with the acylases from Pseudomonas sp. GK16 and C427, but low homology with the others. There is large difference in the N-terminal of alpha-subunit, while the N-terminal of beta-subunit has significant conservation.
Mao
Xiang
X
Shanghai Institute of Plant Physiology and Ecology, Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Zhang
Jing
J
Li
Yong
Y
He
Yu-Jiong
YJ
Wang
En-Duo
ED
Yang
Yun-Liu
YL
Jiang
Wei-Hong
WH
Zhao
Guo-Ping
GP
Chiao
Jui-Sheng
JS
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010399:Penicillin Amidase / Q000235:genetics*; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D010399:Penicillin Amidase; C046586:glutarylamidocephalosporanic acid acylase