title: Chromosomal system for studying AmpC-mediated beta-lactam resistance mutation in Escherichia coli.
abstract: In some enterobacterial pathogens, but not in Escherichia coli, loss-of-function mutations in the ampD gene are a common route to beta-lactam antibiotic resistance. We constructed an assay system for studying mechanism(s) of enterobacterial ampD mutation using the well-developed genetics of E. coli. We integrated the Enterobacter ampRC genes into the E. coli chromosome. These cells acquire spontaneous recombination- and SOS response-independent beta-lactam resistance mutations in ampD. This chromosomal system is useful for studying mutation mechanisms that promote antibiotic resistance.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030-3411, USA. jp691032@bcm.tmc.edu
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Petrosino
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Pendleton
authors.forename: Joel H
authors.initials: JH
authors.lastname: Weiner
authors.forename: Susan M
authors.initials: SM
authors.lastname: Rosenberg
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial; D004754:Enterobacter / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics; D010957:Plasmids; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D047090:beta-Lactams; C071526:AmpR protein, Bacteria; C062460:AmpD protein, Bacteria; D009238:N-Acetylmuramoyl-L-alanine Amidase; C048795:AmpC beta-lactamases; D001618:beta-Lactamases