title: The C-terminal regions of the envelope glycoprotein gp2 of equine herpesviruses 1 and 4 are antigenically distinct.
abstract: The unusual mucin-like high molecular mass (Mr) glycoprotein 2 (gp2) has only been described in the equid alphaherpesviruses, among which there is considerable antigenic cross-reactivity. Equine herpesvirus 1 (EHV-1) gp2 is cleaved into a highly glycosylated N-terminal subunit and a 42 kDa C-terminal cleavage product. In order to investigate their antigenic recognition by horses naturally infected with EHV-1 and/or equine herpesvirus 4 (EHV-4), the C-terminal cleavage product and high Mr gp2 were affinity purified. Cross-reactivity between EHV-1 and EHV-4 was observed for the high Mr gp2 using Western blotting. In contrast only horses with antibodies to EHV-1 detected the 42 kDa EHV-1 gp2 C-terminal cleavage product. This phenomenon was evident in pooled sera from adult horses and also in foals that had demonstrated seroconversion due to EHV-1 infection. The results indicate that the C-terminal region of EHV-1 gp2 is antigenically distinct from that of EHV-4 gp2 and can be detected only after an EHV-1-specific immune response.
authors.affiliation: Faculty of Veterinary Science, The University of Sydney, NSW, Australia.
authors.forename: G S
authors.initials: GS
authors.lastname: Learmonth
authors.forename: D N
authors.initials: DN
authors.lastname: Love
authors.forename: J E
authors.initials: JE
authors.lastname: Wellington
authors.forename: J R
authors.initials: JR
authors.lastname: Gilkerson
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D003429:Cross Reactions; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D023141:Herpesvirus 4, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000276:immunology / Q000821:virology; D006736:Horses; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C077966:glycoprotein 300, Equid herpesvirus 1