In vitro activities of free and lipid formulations of amphotericin B and nystatin against clinical isolates of Coccidioides immitis at various saprobic stages.
We investigated the susceptibilities of hyphal, mixed hyphal, ungerminated arthroconidial, and germinated arthroconidial populations of Coccidioides immitis to lipid formulations of amphotericin B and nystatin and their conventional preparations, utilizing the National Committee for Clinical Laboratory Standards M38-P broth macrodilution method. The differences in effects of the three different growth stages of the saprobic phase of C. immitis on the MIC/minimum lethal concentration (MLC) ratio were not statistically significant for any of the antifungal agents tested. These results suggest that either inocula could be used for in vitro susceptibility studies with C. immitis.
González
Gloria M
GM
Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA. gmglez@yahoo.com.mx
Tijerina
Rolando
R
Sutton
Deanna A
DA
Graybill
John R
JR
Rinaldi
Michael G
MG
D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D003045:Coccidioides / Q000187:drug effects* / Q000254:growth & development*; D003047:Coccidioidomycosis / Q000382:microbiology; D004337:Drug Carriers; D006801:Humans; D008081:Liposomes / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D009761:Nystatin / Q000494:pharmacology*
D000935:Antifungal Agents; D004337:Drug Carriers; D008081:Liposomes; D009761:Nystatin; D000666:Amphotericin B