Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.
We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.
Rosén
M
M
Department of Genetics, Lund University, Sölvegatan, Lund, Sweden.
Kamnert
I
I
Edström
J-E
JE
D000818:Animals; D015152:Blotting, Northern; D002500:Centrifugation, Isopycnic; D002683:Chironomidae / Q000235:genetics*; D004247:DNA; D015342:DNA Probes; D017344:Genes, Insect; D012313:RNA; D012091:Repetitive Sequences, Nucleic Acid; D016615:Telomere
D015342:DNA Probes; D012313:RNA; D004247:DNA