title: Comparison of responses elicited by immunization with a Legionella species common lipoprotein delivered as naked DNA or recombinant protein.
abstract: To evaluate the peptidoglycan-associated lipoprotein (PAL) antigen of Legionella pneumophila as a vaccine candidate, mice were immunized intramuscularly with pcDNA3-PAL and intraperitoneally with recombinant PAL (t-rPAL), which were compared for their ability to induce PAL-specific immune responses. The t-rPAL protein induced PAL-specific IgG antibody production significantly more than did pcDNA3-PAL. The IgG2a and IgG1 production was predominant after pcDNA3-PAL and t-rPAL administration, respectively. In particular, pcDNA3-PAL induced much higher PAL-specific cytotoxic T-lymphocyte responses than did t-rPAL. Furthermore, in vivo, CD19+ B-cell populations were dramatically increased by t-rPAL vaccination, suggesting a B-cell immunomodulatory activity of the lipoprotein. The PAL antigen was also conserved among Legionella species, as determined by PCR and immunoblot analyses. These results support a potential use of the t-rPAL protein and in particular DNA vaccines against Legionella infections.
authors.affiliation: Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul, Korea.
authors.forename: Won Suck
authors.initials: WS
authors.lastname: Yoon
authors.forename: Seol Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Park
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Jeong Im
authors.initials: JI
authors.lastname: Sin
authors.forename: Min Ja
authors.initials: MJ
authors.lastname: Kim
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D029968:Escherichia coli Proteins; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000096:biosynthesis; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007875:Legionella / Q000145:classification / Q000276:immunology*; D016952:Legionella pneumophila / Q000276:immunology; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000235:genetics / Q000276:immunology*; D011509:Proteoglycans; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D007074:Immunoglobulin G; D008074:Lipoproteins; D010457:Peptidoglycan; D011509:Proteoglycans; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C069988:PplA protein, Legionella pneumophila