Cloning of the mouse Sef gene and comparative analysis of its expression with Fgf8 and Spry2 during embryogenesis.
We report the cloning and expression analysis of a mouse gene encoding a novel transmembrane protein. Expression of Sef is similar to that of Fgf8 and Spry2 during early embryogenesis, being prominent in the forebrain, mid-hindbrain boundary, branchial arches, somites, limb bud and tailbud of mouse embryos. These expression profiles indicate that Fgf8, Spry2 and Sef belong to a synexpression group and suggest that these genes may functionally interact during embryonic development. From E12.5 onwards, partially distinct patterns of expression of these genes are observed in the neuroepithelium, sense organs and endodermal-derived organs, that are known sites of expression of other Fgfs.
Lin
Wei
W
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/Université Louis Pasteur, BP163, 1 rue Laurent Fries, 67404 Illkirch cedex, CU de Strasbourg, France.
Fürthauer
Maximillian
M
Thisse
Bernard
B
Thisse
Christine
C
Jing
Naihe
N
Ang
Siew-Lan
SL
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases; D011506:Proteins; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; C496457:Fgf8 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011506:Proteins; C469338:Sef protein, mouse; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D017346:Protein Serine-Threonine Kinases; C118389:Spry2 protein, mouse