Enhanced adenoviral-vector mediated gene transfer using human albumin solder.
OBJECTIVE
Laser tissue welding with human albumin solder (HAS) has been used as an alternative method of wound closure. Adenoviral vectors have been used to introduce various cytokine genes into wounds to accelerate wound closure. In the present study, we were interested in the effect of HAS on adenoviral vector transfer of the beta-galactosidase (beta-gal) gene in vitro and in vivo.


METHODS
3T3 fibroblasts were used to study the effect of HAS on beta-gal gene transfer in vitro. The presence of beta-gal was determined by Western blot, and its activity by a colorimetric assay. A punch biopsy model of wound healing in pigs was used for in vivo experiments.


RESULTS
HAS increased the efficiency of adenoviral-mediated beta-gal transduction and stabilized the adenovirus at room temperature. HAS protected adenovirus from inactivation by laser, both in vitro and in vivo.


CONCLUSIONS
HAS may stabilize adenoviral vectors to deliver cytokine genes in future wound healing experiments.
Rivera
Arnold D C
AD
Department of Urology, The Children's Hospital of New York, Weill Medical College of Cornell University, New York, New York 10021, USA.
Walker
Charles N
CN
Bleustein
Clifford
C
Choi
Benjamin
B
Poppas
Dix P
DP
Felsen
Diane
D
D016475:3T3 Cells; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D007834:Lasers; D051379:Mice; D051381:Rats; D012709:Serum Albumin / Q000008:administration & dosage*; D013552:Swine; D014162:Transfection / Q000379:methods; D014945:Wound Healing; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics*
D012709:Serum Albumin; D001616:beta-Galactosidase