A lipoxygenase inhibitor from Aspergillus niger.
A lipoxygenase-1 (LOX-1) inhibitor was isolated from the fermented broth of Aspergillus niger CFTRI 1105. It was purified, using column and preparative thin layer chromatography. 1H NMR and GC-MS examination revealed the structure of the inhibitor to be 2-(2'-methyl, 4'-hydroxyphenyl), 2-(4"hydroxyphenyl)-propane with a molecular weight of 242 and the molecular formula C,6H18O2. This bisphenol-derivative inhibitor shows 50% inhibition of soybean LOX-I at 0.98 mM concentration. The activity of this inhibitor was compared with commercial bisphenol A and its structural analogues, butylhydroxyanisole and butylhydroxytoluene in an attempt to understand the role of functional groups affecting lipoxygenase activity.
Sekhar
Rao K C
RK
Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, India.
Sekhar
Divakar K C
DK
Appu
Rao A G
RA
Karanth
N G
NG
Sattur
A P
AP
D001234:Aspergillus niger / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight
D016859:Lipoxygenase Inhibitors; D008084:Lipoxygenase