[Plasmid pcDNA3 enhances Ca(2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle of rats].
The effects of plasmid pcDNA3 on Ca(2+) transport of sarcoplasmic reticulum (SR) in ischemic skeletal muscle was investigated. The results show that Ca(2+) transport (including Ca(2+) uptake and Ca(2+) release) rate of SR in ischemic skeletal muscle was markedly increased compared with that in non-ischemic muscle (P<0.01 or P<0.05). After plasmid pcDNA3 bound to the DNA binding proteins of SR, Ca(2+) transport of SR was further increased. The results suggest that the effect of plasmid DNA on the ability of Ca(2+) transport in SR of ischemic skeletal muscle is the same as is observed in normal skeletal muscle. The pathophysiological significance of the present finding deserves further exploration.
Jiang
Z S
ZS
Research Institute of Cardiovascular Diseases, Hengyang Medical College, Hengyang 421001, China.
Zhao
W
W
Yang
Y Z
YZ
Tang
C S
CS
Tang
J
J
Jia
H T
HT
Liu
N K
NK
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004247:DNA / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D017136:Ion Transport; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply*; D010957:Plasmids; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D004268:DNA-Binding Proteins; D004247:DNA; D002118:Calcium