[Cloned goats produced from the somatic cells of an adult transgenic goat].
This study was carried out to examine the effect of different donor cell type and micro-manipulation on the development of reconstituted embryos. Cultured mural cumulus cells or fibroblast cells from an adult transgenic goat expressing human erythropoietin(rhEPO) were used as the donor cells in nuclear transfer experiments. The reconstituted eggs were generated by transferring fibroblast cells or cumulus cells into the perivitelline space of enucleated M II oocytes and then followed by electrofusion and activation. After 6 days' incubation in vivo, the reconstructed embryos developed into morulae or blastocysts were transferred into 6 foster recipients. Two of the foster-mothers were pregnant and gave birth to two offspring, which were derived from the fibroblast cell and cumulus cell, respectively. Fingerprint analysis showed that the PCR-RFLP patterns of the two offspring were identical to that of donor goats. PCR results indicated that these cloned goats carried hEPO gene as same as their donor cells.
Cheng
Yong
Y
Animal Science and Veterinary Medicine College of Yangzhou University, Yangzhou 225009, China. yz.dyx@public.yz.js.cn
Wang
Yu-Ge
YG
Luo
Jin-Ping
JP
Shen
Yu
Y
Yang
Yue-Fei
YF
Ju
Hui-Ming
HM
Zou
Xian-Gang
XG
Xu
Shao-Fu
SF
Lao
Wei-De
WD
Du
Miao
M
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D002459:Cell Fusion / Q000379:methods; D019976:Cloning, Organism; D004624:Embryo Transfer / Q000639:trends; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology*; D006041:Goats / Q000196:embryology* / Q000235:genetics; D006801:Humans; D008845:Microinjections / Q000379:methods; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*
D004921:Erythropoietin