Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents.
With the incorporation of lysozyme during the immobilization step, considerable enhancement of the operational stability of a biosensor has been demonstrated in the case of an immobilized single enzyme (glucose oxidase) system for glucose and multienzyme (invertase, mutarotase and glucose oxidase) system for sucrose. Thus an increased number of repeated analyses of 750 samples during 230 days for glucose and 400 samples during 40 days of operation for sucrose have been achieved. The increased operational stability of immobilized single and multienzyme system, will improve the operating cost effectiveness of the biosensor.
Gouda
M D
MD
Central Food Technological Research Institute, Mysore 570013, India.
Kumar
M A
MA
Thakur
M S
MS
Karanth
N G
NG
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002417:Cattle; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005780:Gelatin / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013395:Sucrose / Q000032:analysis* / Q000737:chemistry
D004800:Enzymes, Immobilized; D012710:Serum Albumin, Bovine; D013395:Sucrose; D005780:Gelatin; D009113:Muramidase; D005947:Glucose