[Effect of intrauterine hepatitis B virus infection on hepatitis B vaccine inoculation in newborns].
OBJECTIVE
To study the effect and the mechanism of peripheral blood nuclear cells (PBMC) invaded by hepatitis B virus (HBV) on the artificial immunization in newborns.


METHODS
Fifty-two newborns, whose mothers were hepatitis B surface antigen (HBsAg) positive, were immunized with hepatitis B immunoglobulin and hepatitis B vaccine (HBVac), and then followed for 7 months. The newborns' serum and PBMC HBV DNA was detected by nested-PCR, hepatitis B surface antibody (HBsAb) was tested with solid phase radioimmunoassay. PBMC from newborn were incubated with PHA and HBsAg. The supernatant interleukin 2 (IL-2) level was measured by enzyme linked immununosorbent assay (ELISA).


RESULTS
The rate of vaccination failure was higher in the infants with PBMC HBV DNA positive than those with negative (P < 0.05). The supernatant IL-2 level was lower in the former than that in the latter and the control (P < 0.05). The level of IL-2 in the immunization failure newborns was lower than that in the vaccination success and the control (P < 0.05).


CONCLUSIONS
The intrauterine PBMC HBV invasion is one of the important causes of vaccination failure in the newborns. PBMC IL-2 autocrine down regulation is closely related to HBV invasion, that may lead to the failure of HBVac inoculation in the newborns.
Meng
Jinlai
J
Department of Obstetrics and Gynecology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Yue
Yafei
Y
Zhang
Shulin
S
D005260:Female; D006509:Hepatitis B / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control* / Q000635:transmission; D017325:Hepatitis B Vaccines / Q000627:therapeutic use*; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008297:Male; D014599:Uterus / Q000821:virology; D014611:Vaccination
D017325:Hepatitis B Vaccines; D007376:Interleukin-2