title: Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens.
abstract: An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydans and A. liquefaciens. Although both lac genes were functionally expressed in both acetic acid bacteria, only a few transformants were able to grow on lactose. However, this ability strictly depended on the presence of a plasmid expressing both lac genes. Mutations in the plasmids and/or in the chromosome were excluded as the cause of growth ability on lactose.
authors.affiliation: Institute for Microbiology, Federal Dairy Research Centre, 24103 Kiel, Germany.
authors.forename: Hesham E
authors.initials: HE
authors.lastname: Mostafa
authors.forename: Knut J
authors.initials: KJ
authors.lastname: Heller
authors.forename: Arnold
authors.initials: A
authors.lastname: Geis
mesh_terms: D000091:Acetobacter / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005822:Genetic Vectors; D020600:Gluconobacter oxydans / Q000235:genetics / Q000254:growth & development; D007763:Lac Operon / Q000235:genetics*; D007785:Lactose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D009004:Monosaccharide Transport Proteins; D010957:Plasmids / Q000235:genetics; D027981:Symporters
chemical_list: D003470:Culture Media; D029968:Escherichia coli Proteins; C050221:LacY protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D027981:Symporters; C021499:lactose permease; D007785:Lactose