title: A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB/c mice.
abstract: This study was conducted to evaluate the immunogenicity of the Brucella abortus lumazine synthase (BLS) gene cloned into the pcDNA3 plasmid, which is driven by the cytomegalovirus promoter. Injection of plasmid DNA carrying the BLS gene (pcDNA-BLS) into BALB/c mice elicited both humoral and cellular immune responses. Antibodies to the encoded BLS included immunoglobulin G1 (IgG1) IgG2a, IgG2b, IgG3, and IgM isotypes. Animals injected with pcDNA-BLS exhibited a dominance of IgG2a over IgG1. In addition, spleen cells from vaccinated animals produced interleukin-2 and gamma interferon but not IL-10 or IL-4 after in vitro stimulation with recombinant BLS (rBLS), suggesting the induction of a Th1 response. Protection was evaluated by comparing the levels of infection in the spleens of vaccinated mice challenged with B. abortus 544. Immunization with pcDNA-BLS- reduced the bacterial burden relative to those in the control groups. Mice immunized with rBLS produced a significant humoral response but did not show a specific cellular response or any protection from challenge. Altogether, these data suggest that pcDNA-BLS is a good immunogen for the production of humoral and cell-mediated responses in mice and is a candidate for use in future studies of vaccination against brucellosis.
authors.affiliation: Facultad de Farmacia y Bioquímica Instituto de Estudios de la Inmunidad Humoral, UNICEN, Tandil, Argentina.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Velikovsky
authors.forename: Juliana
authors.initials: J
authors.lastname: Cassataro
authors.forename: Guillermo H
authors.initials: GH
authors.lastname: Giambartolomei
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Goldbaum
authors.forename: Silvia
authors.initials: S
authors.lastname: Estein
authors.forename: Raul A
authors.initials: RA
authors.lastname: Bowden
authors.forename: Laura
authors.initials: L
authors.lastname: Bruno
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Fossati
authors.forename: Moisés
authors.initials: M
authors.lastname: Spitz
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000201:enzymology / Q000276:immunology*; D002006:Brucellosis / Q000517:prevention & control*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification; D051379:Mice; D008807:Mice, Inbred BALB C; D009097:Multienzyme Complexes / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D007074:Immunoglobulin G; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C044822:6,7-dimethyl-8-ribityllumazine synthase