title: cAMP modulation of Ca(2+)-regulated exocytosis in ACh-stimulated antral mucous cells of guinea pig.
abstract: Effects of cAMP accumulation on ATP-dependent priming and Ca(2+)-dependent fusion in Ca(2+)-regulated exocytosis were examined in antral mucous cells of guinea pigs by using video-enhanced contrast microscopy. The Ca(2+)-regulated exocytosis activated by 1 microM ACh consisted of two phases, an initial transient phase followed by a sustained phase, which were potentiated by cAMP accumulation. Depletion of ATP by 100 microM dinitrophenol (uncoupler of oxidative phosphorylation) or anoxia induced the sustained phase without the initial transient phase in Ca(2+)-regulated exocytosis. However, accumulation of cAMP before depletion of ATP induced and potentiated an initial transient phase followed by a sustained phase in Ca(2+)-regulated exocytosis. This suggests that the initial transient phase of Ca(2+)-regulated exocytosis is induced by fusion of all primed granules maintained by ATP and that accumulation of cAMP accelerates ATP-dependent priming of the exocytotic cycle. Moreover, ACh and Ca(2+) dose-response studies showed that accumulation of cAMP shifted the dose-response curves to the low concentration side, suggesting that it increases Ca(2+) sensitivity in the fusion of the exocytotic cycle. In conclusion, cAMP accumulation increases the number of primed granules and Ca(2+) sensitivity of the fusion, which potentiates Ca(2+)-regulated exocytosis in antral mucous cells.
authors.affiliation: Department of Physiology, Osaka Medical College, 2-7 Daigaku-cho, Takatsuki 569-8686, Japan. takan@art.osaka-med.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakahari
authors.forename: Shoko
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Chikao
authors.initials: C
authors.lastname: Shimamoto
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kojima
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Katsu
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Imai
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology; D004357:Drug Synergism; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D005752:Gastric Mucins / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D006168:Guinea Pigs; D000860:Hypoxia / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D018715:Microscopy, Video; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011706:Pyloric Antrum / Q000166:cytology; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D000318:Adrenergic beta-Agonists; D004140:Dinitrophenols; D005752:Gastric Mucins; D010726:Phosphodiesterase Inhibitors; D014475:Uncoupling Agents; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D007545:Isoproterenol; D000109:Acetylcholine; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine