title: Interleukin-6-producing cells in a human glioblastoma cell line are not affected by ionizing radiation.
abstract: We investigated the production of interleukin 6 (IL-6) by a radioresistant human glioblastoma cell line G5 after single radiation events of 3, 6 and 9 Gy. The total cell number and IL-6 concentration in culture supernatant were assessed 24-96 h after irradiation. The radiation impeded or stopped G5 cell growth in a dose-dependent manner, but unexpectedly did not affect the IL-6 concentration in cell culture media that increased in the same range as in non-irradiated cultures. Furthermore, using flow cytometry, we found that the IL-6 positive cells expansion was unaffected by radiation. These findings suggested that this small (about 1%) fraction of G5 cells, constitutively producing IL-6, is highly radioresistant.
authors.affiliation: Unité d'Immunologie Clinique, Hĵpital Gabriel Montpied, Lyon, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Dubost
authors.forename: Christine
authors.initials: C
authors.lastname: Rolhion
authors.forename: Andrei
authors.initials: A
authors.lastname: Tchirkov
authors.forename: Suzanne
authors.initials: S
authors.lastname: Bertrand
authors.forename: Jacques
authors.initials: J
authors.lastname: Chassagne
authors.forename: Annie
authors.initials: A
authors.lastname: Dosgilbert
authors.forename: Pierre
authors.initials: P
authors.lastname: Verrelle
mesh_terms: D001932:Brain Neoplasms; D002452:Cell Count; D002455:Cell Division / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005909:Glioblastoma; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis*; D011839:Radiation, Ionizing; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
chemical_list: D015850:Interleukin-6