The YbgC protein encoded by the ybgC gene of the tol-pal gene cluster of Haemophilus influenzae catalyzes acyl-coenzyme A thioester hydrolysis.
This paper examines the catalytic function of the protein YbgC, encoded by the ybgC gene of the tol-pal gene cluster in Haemophilus influenzae. The YbgC protein, a homologue of the Pseudomonas sp. strain CBS3 4-hydroxybenzoyl-coenzyme A thioesterase, conserves the active site Asp residue associated with thioesterase activity. The H. influenzae ybgC gene was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and tested for thioesterase activity towards acyl-CoA and acyl-N-acetylcysteamine thioesters. The YbgC protein catalyzes the hydrolysis of short chain aliphatic acyl-CoA thioesters, while the D18N YbgC mutant protein (prepared to serve as a control) does not.
Zhuang
Zhihao
Z
Department of Chemistry, University of New Mexico, Albuquerque, NM 87131, USA.
Song
Feng
F
Martin
Brian M
BM
Dunaway-Mariano
Debra
D
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D006193:Haemophilus influenzae / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013869:Thiolester Hydrolases / Q000235:genetics* / Q000378:metabolism*
D000214:Acyl Coenzyme A; D011994:Recombinant Proteins; C009061:propionyl-coenzyme A; D013869:Thiolester Hydrolases