title: The eye lens protein alphaA-crystallin of the blind mole rat Spalax ehrenbergi: effects of altered functional constraints.
abstract: The rudimentary eyes of the mole rat Spalax ehrenbergi have lost their visual function, but are still required for the control of circadian rhythms. It has previously been found that alphaA-crystallin, a major eye lens protein in other mammals, evolved much faster in the mole rat than in rodents with normal vision. Yet, although mole rat alphaA-crystallin seems superfluous as a lens protein, its rate of change is still much slower than that of pseudogenes, suggesting some remaining function. The authors therefore studied the structure and function of recombinant mole rat alphaA-crystallin. Circular dichroism (CD), tryptophan fluorescence and gel permeation analyses indicated that the overall structure and stability of mole rat alphaA-crystallin are comparable to that of rat alphaA-crystallin. However, the chaperone-like activity of mole rat alphaA-crystallin is considerably lower than that of its rat orthologue. Two-dimensional NMR spectroscopy of mole rat alphaA-crystallin suggests that this may be in part due to a diminished flexibility of the C-terminal extension, which is thought to be important for the chaperoning capacity. Overall, mole rat alphaA-crystallin appears to still be a viable protein, confirming that it has some as yet elusive role, despite the loss of its primary lens function.
authors.affiliation: Department of Biochemistry, University of Nijmegen, 6500 HB Nijmegen, The Netherlands.
authors.forename: Ronald H P H
authors.initials: RH
authors.lastname: Smulders
authors.forename: Marjon A M
authors.initials: MA
authors.lastname: van Dijk
authors.forename: Simon
authors.initials: S
authors.lastname: Hoevenaars
authors.forename: Robyn A
authors.initials: RA
authors.lastname: Lindner
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
authors.forename: Wilfried W
authors.initials: WW
authors.lastname: de Jong
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D003459:Crystallins / Q000737:chemistry* / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008850:Microphthalmos / Q000378:metabolism*; D019577:Mole Rats / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D003459:Crystallins; D011994:Recombinant Proteins