[The effect of cyclic nucleotides on the membrane potential of intact endothelium of isolated rabbit aorta].
The influence of intracellular cyclic nucleotides concentration increase on the membrane potential of intact endothelium of isolated rabbit aorta has been investigated. Both applications of bucladesine, an adenosine 3',5'-cyclic monophosphate (cAMP) membrane permeable analogue, and phoshophodiesterase blockers 3-isobutyl-1-methylxanthine (IBMX) and theophylline depolarized endothelial membrane. IBMX-induced depolarization was significantly smaller then theophylline-induced depolarization. It could be explained by the existence of IBMX-insensitive phosphodiesterases. Non-selective protein kinase blocker H-7 did not affect on the both IBMX-stimulated and non-stimulated membrane potential of aortic endothelium. It has been shown that the IBMX-induced depolarization and ATP-induced depolarization correlate with high correlation coefficient.
Iarots'kyĭ
V V
VV
A. A. Bogomoletz Institute of Physiology, National Academy of Science of Ukraine, Kiev.
Sahach
V F
VF
Marchenko
S M
SM
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects*; D009712:Nucleotides, Cyclic / Q000378:metabolism / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D013806:Theophylline / Q000494:pharmacology
D009712:Nucleotides, Cyclic; D010726:Phosphodiesterase Inhibitors; D003994:Bucladesine; D013806:Theophylline; D015056:1-Methyl-3-isobutylxanthine