title: Selective induction of apoptosis by ar-turmerone isolated from turmeric (Curcuma longa L) in two human leukemia cell lines, but not in human stomach cancer cell line.
abstract: We have investigated the effects of ar-turmerone isolated from turmeric (Curcuma longa L) on DNA of human leukemia cell lines, Molt 4B, HL-60 and stomach cancer KATO III cells. It was found that selective induction of apoptosis by ar-turmerone was observed in human leukemia Molt 4B and HL-60 cells, but not in human stomach cancer KATO III cells. Morphological changes showing apoptotic bodies were observed in the human HL-60 and Molt 4B cells treated with ar-turmerone. The fragmentation of DNA by ar-turmerone to oligonucleosomal-sized fragments that is a characteristic of apoptosis was observed to be concentration- and time-dependent in Molt 4B and HL-60 cells, but not in KATO III cells. The data of the present study show that the suppression by ar-turmerone of growth of these leukemia cell lines results from the induction of apoptosis by this compound.
authors.affiliation: Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan.
authors.forename: Yue
authors.initials: Y
authors.lastname: Aratanechemuge
authors.forename: Takashi
authors.initials: T
authors.lastname: Komiya
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Moteki
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Katsuzaki
authors.forename: Kunio
authors.initials: K
authors.lastname: Imai
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Hibasami
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D030024:Curcuma / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D012717:Sesquiterpenes; D013274:Stomach Neoplasms / Q000473:pathology*; D014050:Toluene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured
chemical_list: D007659:Ketones; D012717:Sesquiterpenes; C078098:ar-turmerone; D014050:Toluene