Effects of lyophilized black raspberries on azoxymethane-induced colon cancer and 8-hydroxy-2'-deoxyguanosine levels in the Fischer 344 rat.
This study examined the effects of lyophilized black raspberries (BRB) on azoxymethane (AOM)-induced aberrant crypt foci (ACF), colon tumors, and urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in male Fischer 344 rats. AOM was injected (15 mg/kg body wt i.p.) once per week for 2 wk. At 24 h after the final injection, AOM-treated rats began consuming diets containing 0%, 2.5%, 5%, or 10% (wt/wt) BRB. Vehicle controls received 5% BRB or diet only. Rats were sacrificed after 9 and 33 wk of BRB feeding for ACF enumeration and tumor analysis. ACF multiplicity decreased 36%, 24%, and 21% (P < 0.01 for all groups) in the 2.5%, 5%, and 10% BRB groups, respectively, relative to the AOM-only group. Total tumor multiplicity declined 42%, 45%, and 71% (P < 0.05 for all groups). Although not significant, a decrease in tumor burden (28%, 42%, and 75%) was observed in all BRB groups. Adenocarcinoma multiplicity decreased 28%, 35%, and 80% (P < 0.01) in the same treatment groups. Urinary 8-OHdG levels were reduced by 73%, 81%, and 83% (P < 0.01 for all groups). These results indicate that BRB inhibit several measures of AOM-induced colon carcinogenesis and modulate an important marker of oxidative stress in the Fischer 344 rat.
Harris
G K
GK
Department of Food Sciences and Technology, College of Food, Agriculture, and Environmental Sciences, Ohio State University, Columbus, OH 43210, USA.
Gupta
A
A
Nines
R G
RG
Kresty
L A
LA
Habib
S G
SG
Frankel
W L
WL
LaPerle
K
K
Gallaher
D D
DD
Schwartz
S J
SJ
Stoner
G D
GD
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000230:Adenocarcinoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000236:Adenoma / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D000872:Anthocyanins / Q000032:analysis; D016588:Anticarcinogenic Agents / Q000032:analysis; D001397:Azoxymethane; D002118:Calcium / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003373:Coumaric Acids / Q000032:analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D004032:Diet; D004610:Ellagic Acid / Q000032:analysis; D005612:Freeze Drying; D005638:Fruit; D018384:Oxidative Stress; D051381:Rats; D011916:Rats, Inbred F344; D027824:Rosaceae / Q000737:chemistry; D012855:Sitosterols / Q000032:analysis
D000872:Anthocyanins; D016588:Anticarcinogenic Agents; D003373:Coumaric Acids; D012855:Sitosterols; D004610:Ellagic Acid; C025473:gamma-sitosterol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002784:Cholesterol; C004999:ferulic acid; D003849:Deoxyguanosine; D001397:Azoxymethane; D002118:Calcium