title: Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on serum inhibin concentrations and inhibin immunostaining during follicular development in female Sprague-Dawley rats.
abstract: Intact and hypophysectomized immature rats were pretreated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 0 or 32 microg/kg p.o.) and sacrificed throughout synchronized follicular development (0, 12, 24, 48, and 72 h after equine chorionic gonadotropin, eCG). TCDD administration to intact rats resulted in a premature elevation of serum FSH and LH by 12 h post-eCG. In intact rats pretreated with TCDD, the intensity of ovarian immunoreactivity for inhibin and the number of ovarian follicles staining for inhibin in midsaggital ovarian sections were decreased at the time of eCG administration (24 h post-TCDD) in comparison to controls. However, this decreased ovarian staining for inhibin was not associated with alterations in serum inhibin concentrations. Serum inhibin was suppressed in TCDD-treated rats when compared to intact controls only at 24 h post-eCG. Hypophysectomized animals exhibited no effect of TCDD on serum inhibin at any timepoint but did have decreased estradiol concentrations during follicular development. In summary, TCDD reduced serum concentrations of inhibin after the premature increases in FSH and LH suggesting that inhibin is not important in the initial elevation of FSH following exposure to TCDD.
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City 66160, USA.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Petroff
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: FangXiong
authors.initials: F
authors.lastname: Shi
authors.forename: Deok-Soo
authors.initials: DS
authors.lastname: Son
authors.forename: Katherine F
authors.initials: KF
authors.lastname: Roby
authors.forename: Karl K
authors.initials: KK
authors.lastname: Rozman
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
authors.forename: Paul F
authors.initials: PF
authors.lastname: Terranova
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007016:Hypophysectomy; D007150:Immunohistochemistry; D007265:Inhibins / Q000097:blood / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010060:Ovulation / Q000502:physiology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013723:Teratogens / Q000633:toxicity*
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone