Identification of genes associated with head and neck carcinogenesis by cDNA microarray comparison between matched primary normal epithelial and squamous carcinoma cells.
In order to identify genes involved in head and neck carcinogenesis, we compared the gene expression profile in matched primary normal epithelial cells and primary head and neck cancer cells from the same patients. A cDNA microarray analysis consisting of 12 530 human genes revealed significant changes in the expression of 213 genes, with 91 genes being up-regulated and 122 being down-regulated. This comprehensive list of genes includes those associated with signal transduction (growth factors), cell structure, cell cycle, transcription, apoptosis, and cell-cell adhesion. Further analysis of nine genes involved in cell-cell interaction, using Western blot and/or reverse transcription (RT)-PCR of four paired cell lines supported the reliability of our microarray analysis. More specifically, our study provides the first evidence that claudin-7 and connexin 31.1 are down-regulated in head and neck squamous cell carcinomas (HNSCC) compared to normal cells. These findings provide a large body of information regarding gene expression profiles associated with head and neck carcinogenesis, and also represent a source of potential targets for HNSCC prevention and/or therapeutics.
Al Moustafa
Ala-Eddin
AE
Lady Davis Institute for Medical Research of the Sir Mortimer B Davis-Jewish General Hospital, Department of Medicine, McGill Center for Translational Research in Cancer, 3755, Ch. de la Cote Ste-Catherine, Montreal, Quebec, Canada H3T 1E2.
Alaoui-Jamali
Moulay A
MA
Batist
Gerald
G
Hernandez-Perez
Maite
M
Serruya
Corinne
C
Alpert
Lesley
L
Black
Martin J
MJ
Sladek
Robert
R
Foulkes
William D
WD
D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D017630:Connexins / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012334:RNA, Neoplasm / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017630:Connexins; D017931:DNA Primers; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C076274:connexin 31.1