Probing domain swapping for the neuronal SNARE complex with electron paramagnetic resonance.
Highly conserved soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins control membrane fusion at synapses. The target plasma membrane-associated SNARE proteins and the vesicle-associated SNARE protein assemble into a parallel four-helix bundle. Using a novel EPR approach, it is found that the SNARE four-helix bundles are interconnected via domain swapping that is achieved by substituting one of the two SNAP-25 helices with the identical helix from the second four-helical bundle. Domain swapping is likely to play a role in the multimerization of the SNARE complex that is required for successful membrane fusion. The new EPR application employed here should be useful to study other polymerizing proteins.
Kweon
Dae-Hyuk
DH
Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA.
Chen
Yong
Y
Zhang
Fan
F
Poirier
Michelle
M
Kim
Chang Sup
CS
Shin
Yeon-Kyun
YK
D003497:Cyclic N-Oxides / Q000737:chemistry; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005583:Fourier Analysis; D008561:Membrane Fusion / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D050600:SNARE Proteins; D013113:Spin Labels; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins
D003497:Cyclic N-Oxides; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; D013113:Spin Labels; D050825:Synaptosomal-Associated Protein 25; D033921:Vesicular Transport Proteins