title: [The RNA polymerase structure-activity studies (1962-2001)].
abstract: The study of RNA polymerase initiated by R.B. Khesin is conducted for about forty years at the laboratory founded by him (since 1989, in collaboration with A. Goldfarb's laboratory). Genetic methods are used in combination with methods of the specific chemical crosslinks of nucleic acids with proteins. The paper assesses the main results of the study in comparison with the X-ray crystallographic data of high resolution obtained recently. A short comparative summary of the RNA polymerase structure has been done for bacteria, archaebacteria, and eukaryotic organelles and nuclei. A brief history of the RNA polymerase study is also presented.
authors.affiliation: Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia. nikiforo@phri.nyu.edu
authors.forename: V G
authors.initials: VG
authors.lastname: Nikiforov
mesh_terms: D018360:Crystallography, X-Ray / Q000266:history / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000266:history* / Q000378:metabolism; D049673:History, 20th Century; D049674:History, 21st Century; D008957:Models, Genetic; D012426:Russia; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases