title: Cardiac arrest cerebral ischemia model in mice failed to cause delayed neuronal death in the hippocampus.
abstract: Global cerebral ischemia models for genetically engineered mice are of particular importance for the study of delayed neuronal death, but have been complicated by variability of vascular anatomy. Here we developed a 5-min cardiac arrest model that was not affected by vascular anatomy, and evaluated the hippocampal neuronal injury in BL/6 and SV129 mice. Despite prolonged anoxic depolarization for approximately 7 min, however, no consistent ischemic neuronal injury was noted in the CA1 sector of the hippocampus in both strains. Thus, our observations suggested that murine hippocampal neurons are relatively resistant to ischemia compared with those in other rodents.
authors.affiliation: Department of Neurosurgery, Faculty of Medicine, University of Tokyo, Japan. kawahara-tky@umin.ac.jp
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kawahara
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kawai
authors.forename: Tomikatsu
authors.initials: T
authors.lastname: Toyoda
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakatomi
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Furuya
authors.forename: Takaaki
authors.initials: T
authors.lastname: Kirino
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000503:physiopathology; D016923:Cell Death / Q000502:physiology; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000473:pathology / Q000503:physiopathology; D006624:Hippocampus / Q000166:cytology / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology / Q000473:pathology*