title: Ultrastructural characteristics of the round window membrane during pneumococcal otitis media in rat.
abstract: To understand better the pathogenesis of inner ear (IE) damage caused by otitis media (OM), the round window membrane(RWM) structure was investigated in a rat model for pneumococcal otitis media (POM). The RWM of 25 rats were evaluated light and electron microscopically on 1 day, 3 days, 6 days, 10 days, and 20 days after the unilateral inoculation of type 3 pneumococcus suspension into their middle ear cavities. The thickness of the RWM increased in various stages of the pneumococcus-evoked otitis media, compared with that of the normal. The thickening was most pronounced on day 1, being about 4 to 5 times greater than that of the normal RWM. All layers of the RWM were affected by the pneumococcal infection, but the major changes were confined to the subepithelial space close to the basement membrane (BM). Together with alterations to the BM, the most distinct pathological features were characterized by an increase and hypertrophy of fibroblasts in association with abundant collagen fibers. Elastic fibers observed close to the inner mesothelial layer under a high power magnification also increased during the experiment. These results will be relevant to a better understanding of the histologic implication of RWM in stages of acute otitis media involving pneumococcus-evoked otitis media.
authors.affiliation: Department of Otorhinolaryngology, Chonbuk National University Hospital, Chonju, Korea. yjyoon@moak.chonbuk.ac.kr
authors.forename: Yong-Joo
authors.initials: YJ
authors.lastname: Yoon
authors.forename: Sten
authors.initials: S
authors.lastname: Hellstrom
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D018450:Disease Progression; D004432:Ear, Middle / Q000473:pathology / Q000648:ultrastructure*; D008297:Male; D010033:Otitis Media / Q000473:pathology*; D011008:Pneumococcal Infections / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013296:Streptococcus pneumoniae; D013997:Time Factors