title: Lung expression of cytochrome P450 1A1 as a possible biomarker of exposure to diesel exhaust particles.
abstract: Polycyclic aromatic hydrocarbons (PAH) and reactive oxygen species (ROS) derived from diesel exhaust particles (DEP) are implicated in the pathophysiology of respiratory diseases. Cytochrome P450 (Cyp) 1A1 can be induced by several kinds of PAH and produce ROS. We determined whether acute inhalation exposure to DEP induced the expression of Cyp 1A1 in murine lung. Intratracheal instillation of DEP dose-dependently increased the lung expression of Cyp 1A1 at the levels of both mRNA and protein, whereas DEP decreased expression in the lung of aryl hydrocarbon receptors in a dose-dependent manner. In contrast, charcoal particles as the control did not affect the expression of these molecules. These results suggest that the lung expression of Cyp 1A1 can be a biomarker of acute inhalation exposure to DEP and may be implicated in an accelerated production of ROS and the subsequent aggravation of lung injury.
authors.affiliation: CREST, JST (Japan Science and Technology Corporation), Tokyo, Japan. htakano@nies.go.jp
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Takano
authors.forename: Rie
authors.initials: R
authors.lastname: Yanagisawa
authors.forename: Takamichi
authors.initials: T
authors.lastname: Ichinose
authors.forename: Kaori
authors.initials: K
authors.lastname: Sadakane
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Inoue
authors.forename: Sei-ichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda
authors.forename: Shin
authors.initials: S
authors.lastname: Yoshino
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Morita
mesh_terms: D000818:Animals; D015415:Biomarkers; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D019570:Inhalation Exposure; D007442:Intubation, Intratracheal; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D001335:Vehicle Emissions; D019363:Cytochrome P-450 CYP1A1