[The activation of vagus afferent in response to lipopolysaccharide   the role of interleukin-1].
To study the possibility of activation of vagus afferent in response to lipopolysaccharide (LPS) through interleukin-1 (IL-1), Wistar rats were randomly divided into LPS group and saline (NS) group. The expression of c-Fos, CD14 and Mac-1 were detected by immunohistochemistry staining. IL-1 bioactivity was determined by L929 cell proliferation. The expression of IL-1R I mRNA was detected by in situ hybridization. Our results showed that there were some c-Fos protein expression positive neurons in the nodose ganglion in LPS group, but no c-Fos protein expression positive neurons in the nodose ganglion in control group. The number of macrophages (M phi) in the connective tissue surrounding the abdominal vagus increased significantly in response to LPS i.p. Forty-five minutes after the application of LPS, the IL-1 bioactivity in the supernatant of M phi was increased. Positive IL-1R mRNA neurons were also observed in the nodose ganglion in the LPS group. The results indicate that vagus afferent is activated in response to LPS and that IL-1 production might be involved in the activation of vagus afferent.
Lu
Xiu-Ying
XY
Norman Bethune National Peace Hospital, Shijiazhuang 050082. xiuyinglu@joymail.com
Yang
Gui-Zhen
GZ
Sun
Hui-Chen
HC
D000818:Animals; D002478:Cells, Cultured; D007375:Interleukin-1 / Q000096:biosynthesis / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D014630:Vagus Nerve / Q000187:drug effects* / Q000378:metabolism
D007375:Interleukin-1; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos