[Serial determination of crimidine by HPLC/SE/SM in a patient ingesting a "mouse trap"].
Crimidine (2 chloro, 4 methyl, 6 dimethyl amidopyrine) is a synthetic rodenticide which causes acute poisonings after oral ingestion in human. Major toxic effects are consciousness disorders, hypertonic coma and convulsions. Toxic level in human is about 5 mg/Kg. An intoxication case is reported. Five serums collected at different times were analyzed with HPLC/ES/MS. Crimidine was extracted with ethylacetate with recovery over 80%. Linearity was up to 800 micrograms/L. LOQ and LOD were 0.5 and 0.3 microgram/L respectively. The coefficients of variation were less than 10% for repeatability and reproductibility. Serum levels varied from 368 micrograms/L for H0 to 64 micrograms/L for H10 and elimination of crimidine was linear in time.
Besnard
T
T
Unité Fonctionnelle de Toxicologie, Laboratoire de Biochimie et de Biologie Moléculaire, CHRU de Lille-59037 Lille.
Sadeg
N
N
Ricart
N
N
Richecoeur
J
J
Nisse
P
P
Vinner
E
E
Houdret
N
N
Humbert
L
L
Ihermitte
M
M
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D011743:Pyrimidines / Q000097:blood* / Q000493:pharmacokinetics / Q000506:poisoning*; D012378:Rodenticides / Q000097:blood* / Q000493:pharmacokinetics / Q000506:poisoning*; D013997:Time Factors
D011743:Pyrimidines; D012378:Rodenticides; C004579:crimidine