[Registration by dynamic phase microscopy of characteristic frequencies of phase height fluctuations of the myelin nerve fiber at rest and under stimulation].
The method of dynamic phase microscopy was used to study the dynamics of changes in the structure of paranodal and nodal regions of a myelin nerve fiber of brown frog Rana temporaria at rest and under stimulation. Regular structural changes with frequencies of 5.3 and 10.8 Hz in the nodal region of the myelin nerve fiber were detected. A rhythmic excitation leads to additional changes in the structure of the nodal region with a new frequency of 5.6 Hz. It is likely that the regular changes in the nodal region of the myelin nerve induced by rhythmic excitation are due to slow changes in the axolemma (changes in the mode of lateral diffusion of membrane phospholipids), induced by developing trace changes in the membrane potential of the axolemma. The fact that these changes do not occur in the paranodal region of the fibre may indicate either the localization of regular structural changes in the axolemma or the difficulties that arise during the registration of the useful signal in the vicinity of myelin by this method.
Maksimov
G V
GV
Biological Department, Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119899 Russia.
Nikandrov
S L
SL
Lazareva
E S
ES
Tychinskiĭ
V P
VP
D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008858:Microscopy, Phase-Contrast; D009413:Nerve Fibers, Myelinated / Q000502:physiology*; D011896:Rana temporaria