Intramolecular DNA coiling mediated by metallo-supramolecular cylinders: differential binding of P and M helical enantiomers.
We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M(-1) and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.
Meistermann
Isabelle
I
Department of Chemistry, University of Warwick, Coventry CV4 7AL, United Kingdom.
Moreno
Virtudes
V
Prieto
Maria J
MJ
Moldrheim
Erlend
E
Sletten
Einar
E
Khalid
Syma
S
Rodger
P Mark
PM
Peberdy
Jemma C
JC
Isaac
Christian J
CJ
Rodger
Alison
A
Hannon
Michael J
MJ
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D008024:Ligands; D008670:Metals / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013053:Spectrophotometry; D013696:Temperature
D008024:Ligands; D008670:Metals; D004247:DNA; D007501:Iron