title: Enhanced expression of p53 in reactive astrocytes following transient focal ischemia.
abstract: The present study used immunohistochemistry to investigate p53 expression in rat brain following transient occlusion of the middle cerebral artery. In the control group, no p53-immunoreactive cells were found in any region of the central nervous system. P53 expression in reactive astrocytes was not obvious in the forebrain one day or three days following ischemic insults. Seven days following ischemic injury, increased expression of p53 was clearly detectable in reactive astrocytes in affected cortical regions, such as forelimb area, hindlimb area, and parietal cortex. At seven days of recirculation, there was also a significant increase in the number of p53-immunoreactive neurons in the cerebral cortex, striatum, and hippocampal CA1-3 regions. Although the present study has not addressed multiple mechanisms contributing to cell death following ischemic injury, the first demonstration of a significant increase in p53 expression in glial cells may prove useful for future investigations of the pathophysiology of ischemia.
authors.affiliation: Department of Anatomy, Seoul National University College of Medicine, Korea. yoonhee2@snu.ac.kr
authors.forename: Yoon Hee
authors.initials: YH
authors.lastname: Chung
authors.forename: Chung Min
authors.initials: CM
authors.lastname: Shin
authors.forename: Myeung Ju
authors.initials: MJ
authors.lastname: Kim
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Gook
authors.initials: G
authors.lastname: Kim
authors.forename: Choong Ik
authors.initials: CI
authors.lastname: Cha
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism*; D001921:Brain / Q000378:metabolism / Q000502:physiology; D001923:Brain Chemistry / Q000502:physiology; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D016159:Tumor Suppressor Protein p53