title: [Experimental study on the change and effect of TGF-beta 1 after skin expansion].
abstract: OBJECTIVE
  To study the injury process of the skin due to expansion.
  
  
  METHODS
  New Zealand rabbits were divided into 4 groups: rapid expansion and slow expansion as well as two control groups. The changes of skin TGF-beta 1 were observed immediately after expansion and at 1, 12, 24 weeks after expansion. Immunohistochemistry and in situ hybridization technique were applied.
  
  
  RESULTS
  TGF-beta 1 increased in the skin immediately after expansion. In groups of rapid expansion, TGF-beta 1 increased faster and then decreased also faster than slow expansion groups. The results from immunohistochemistry and in situ hybridization were almost same.
  
  
  CONCLUSIONS
  Expansion resulted in skin injury. Rapid expansion injured the skin more seriously than slow expansion. TGF-beta 1 may be the main regulating factor to repairing process.
authors.affiliation: Department of Plastic Surgery, Ninth Hospital of Shanghai, Second Medical University, Shanghai 200011, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Fan
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D062109:Dermatologic Surgical Procedures; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D011817:Rabbits; D013997:Time Factors; D015626:Tissue Expansion; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000502:physiology*
chemical_list: D016212:Transforming Growth Factor beta