An experimental model of gastroschisis using fetoendoscopy: preliminary results and technical considerations.
BACKGROUND
The aim of this study was to show the feasibility of an experimental model of gastroschisis using fetoendoscopic surgery on sheep fetuses, and also to perform amnioinfusion until delivery using an in-dwelling intraamniotic catheter.


METHODS
We analyzed the data from 18 pregnant ewes having 26 fetuses, which underwent surgery at 80 days of gestation (full term, 145 days). The fetendo technique was used to create a gastroschisis in 15 fetuses. The fetal abdominal wall was opened on the left side of the cord using scissors. The omentum and the intestinal loops were eviscerated using atraumatic forceps. Eleven fetuses were used as a control group. Twenty-one fetuses underwent amnioinfusion; a simple exteriorized catheter was used in seven cases and an intraamniotic catheter with an implantable port was used in the other 14. All ewes and fetuses were killed at the end of the experiment by an intravenous injection of pentotal; thereafter, the fetuses underwent necropsy.


RESULTS
Twelve fetuses died and 14 survived (53.8%); seven of the 15 that underwent gastroschisis survived (46.7%). An amniotic infection occurred in nine fetuses (34.6%); of these, six died and three that were administered antibiotics survived.


CONCLUSIONS
Our experience shows that this experimental model of gastroschisis is feasible and reproducible, and that a repeated amnioinfusion can be performed with an in-dwelling catheter in pregnant ewes. The use of an implantable port is safer than a simple exteriorized catheter.
Guys
J M
JM
Department of Pediatric Surgery, La Timone Hospital, 1 Boulevard Jean Moulin, 13005 Marseille, France.
Esposito
C
C
Simeoni
J
J
D'Ercole
C
C
Paut
O
O
Bouzid
A
A
Boubli
L
L
D000818:Animals; D004195:Disease Models, Animal; D004724:Endoscopy / Q000379:methods*; D005260:Female; D005333:Fetus / Q000601:surgery; D020139:Gastroschisis / Q000175:diagnosis* / Q000601:surgery*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012756:Sheep