title: Cytotoxic dammarane glycosides from processed ginseng.
abstract: Steaming ginseng at high temperature increased its cytotoxicity to SK-Hep-1 hepatoma cancer cells. HPLC separation and fractionation followed by MTT assay revealed that ginsenosides Rg3, Rg5, Rk1, Rs5, and Rs4 are the active principles. Their 50% growth inhibition concentration (GI50) values were 41, 11, 13, 37, and 13 microM, respectively. Cisplatin had a GI50 of 84 microM in the same assay conditions.
authors.affiliation: College of Pharmacy, Seoul National University, South Korea.
authors.forename: Il Ho
authors.initials: IH
authors.lastname: Park
authors.forename: Long Zhu
authors.initials: LZ
authors.lastname: Piao
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
authors.forename: Yong Jae
authors.initials: YJ
authors.lastname: Lee
authors.forename: Sool Yeon
authors.initials: SY
authors.lastname: Cho
authors.forename: Man Ki
authors.initials: MK
authors.lastname: Park
authors.forename: Jeong Hill
authors.initials: JH
authors.lastname: Park
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000494:pharmacology; D036145:Ginsenosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008113:Liver Neoplasms / Q000473:pathology; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D013696:Temperature; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000097669:Dammaranes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002945:Cisplatin; D036145:Ginsenosides; D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes; D000097669:Dammaranes