[Clinical evaluation of the structural characteristics of peripheral blood leukocyte DNA in patients with viral hepatitis B].
The DNA structure of peripheral blood leukocytes in healthy donors and in viral hepatitis B patients was studied by the rate of the alkaline denaturation of DNA in cell lysates. An increased rate of the DNA alkaline denaturation of cell lysates was established, which was indicative of the damages in their DNA. The most pronounced damages of DNA were found in granulocytes of patients with highly active chronic hepatitis and hepatic cirrhosis, especially in cases of simultaneous delta-virus infection. The damage of leukocyte DNA reflected probably the accumulation of cells, committed to apoptosis, in peripheral blood. Apoptosis may be induced by cytotoxic lymphocytes recognizing HBV-infected cells.
Maksimov
S L
SL
State Medico-Stomatological University, Central Research Institute of Epidemiology, Research Institute of Medical Ecology, Moscow, Russia.
Zmyzgova
A V
AV
Moskaleva
E Iu
EIu
Kizenko
O A
OA
D017209:Apoptosis / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage; D006509:Hepatitis B / Q000097:blood* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D009691:Nucleic Acid Denaturation
D004247:DNA