title: [The effects of wild-type PTEN transfection on gene expressions of glioblastomas].
abstract: OBJECTIVE
  To study the effects of wild-type PTEN on gene expressions of glioblastomas.
  
  
  METHODS
  Glioblastoma U87MG cells, which express inactivated PTEN, were transfected with wild-type PTEN constructs and stable transfected clones were selected. Then, cDNA microarray analyses were used to identify differentially expressed genes in wild-type PTEN transfected cells and control cells.
  
  
  RESULTS
  Transfected wild-type PTEN inhibited the proliferation of U87MG. By cDNA microarray analyses, 89 cDNA clones were identified, which were differentially expressed in wild-type PTEN transfected cells and control cells. Among these genes, 13 genes were unknown and 76 genes were known genes, including glial fibrillary acidic protein, p21/WAF1, human TGF-beta inducible early protein, human DNA fragmentation factor 45 etc.
  
  
  CONCLUSIONS
  Wild-type PTEN can affect the expressions of multiple genes, by which it regulates the proliferation, differentiation and apoptosis of glioblastomas.
authors.affiliation: Department of Pathology, Health Science Center, Beijing University, Beijing 100083, China. tianxinxia@yahoo.com
authors.forename: Xinxia
authors.initials: X
authors.lastname: Tian
authors.forename: Jinfen
authors.initials: J
authors.lastname: Wang
authors.forename: Jinxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Chung Sean
authors.initials: CS
authors.lastname: Pang
authors.forename: N G
authors.initials: NG
authors.lastname: Ho-Keung
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human