[Extraction of the salt-tolerant cDNA in mangrove Avicennia marina by mRNA differential display].
RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.
Zhou
Han-Tao
HT
School of Life Sciences, Xiamen University, Xiamen 361005, China. htzhou@public.xm.fj.cn
Lin
Peng
P
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D018744:DNA, Plant; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D019684:Magnoliopsida; D020714:Maximum Tolerated Dose; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D012492:Salts / Q000494:pharmacology; D017422:Sequence Analysis, DNA / Q000379:methods
D018076:DNA, Complementary; D018744:DNA, Plant; D012492:Salts