title: An O-glycoside of sialic acid derivative that inhibits both hemagglutinin and sialidase activities of influenza viruses.
abstract: The compound Neu5Ac3alphaF-DSPE (4), in which the C-3 position was modified with an axial fluorine atom, inhibited the catalytic hydrolysis of influenza virus sialidase and the binding activity of hemagglutinin. The inhibitory activities to sialidases were independent of virus isolates examined. With the positive results obtained for inhibition of hemagglutination and hemolysis induced by A/Aichi/2/68 virus, the inhibitory effect of Neu5Ac3alphaF-DSPE (4) against MDCK cells was examined, and it was found that 4 inhibits the viral infection with IC50 value of 5.6 microM based on the cytopathic effects. The experimental results indicate that compound 4 not only inhibits the attachment of virus to the cell surface receptor but also disturbs the release of the progeny viruses from infected cells by inhibiting both hemagglutinin and sialidase of the influenza viruses. The study suggested that the compound is a new class of bifunctional drug candidates for the future chemotherapy of influenza.
authors.affiliation: Department of Biochemistry, University of Shizuoka School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka-shi 422-8526, Japan.
authors.forename: Chao-Tan
authors.initials: CT
authors.lastname: Guo
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
authors.forename: Osamu
authors.initials: O
authors.lastname: Kanie
authors.forename: Kennedy Francis
authors.initials: KF
authors.lastname: Shortridge
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Daisei
authors.initials: D
authors.lastname: Miyamoto
authors.forename: Kazuya I-P Jwa
authors.initials: KI
authors.lastname: Hidari
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D001665:Binding Sites; D002236:Carbohydrate Conformation; D004912:Erythrocytes / Q000276:immunology; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000201:enzymology*; D007700:Kinetics; D008081:Liposomes; D008958:Models, Molecular; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D012794:Sialic Acids / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D006027:Glycosides; D008081:Liposomes; D012794:Sialic Acids; D009439:Neuraminidase