The nucleolar localization domain of the catalytic subunit of human telomerase.
Telomerase is the enzyme essential to complete the replication of the terminal DNA of most eukaryotic chromosomes. In humans, this enzyme is composed of the telomerase reverse transcriptase (hTERT) and telomerase RNA (hTR) subunits. hTR has been found in the nucleolus, a site of assembly of ribosomes as well as other ribonucleoproteins (RNPs). We therefore tested whether the hTERT component is also found in the nucleolus, where it could complex with the hTR RNA to form a functional enzyme. We report here that hTERT does indeed localize to the nucleolus, and we mapped the domain responsible for this localization to the hTR-binding region of the protein by deletion analysis. Substitution mutations in two of the three conserved hTR-binding domains in this nucleolar localization domain (NoLD) abolished nucleolar localization. However, another mutation that impeded hTR binding did not alter this subcellular localization. Additionally, wild type hTERT was detected in the nucleolus of cells that failed to express hTR. Taken together, we propose that the nucleolar localization of hTERT involves more than just the association with the hTR subunit. Furthermore, the coincidental targeting of both the hTR and hTERT subunits to the nucleolus supports the premise that the assembly of telomerase occurs in the nucleolus.
Etheridge
Katherine T
KT
Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Banik
Soma S R
SS
Armbruster
Blaine N
BN
Zhu
Yusheng
Y
Terns
Rebecca M
RM
Terns
Michael P
MP
Counter
Christopher M
CM
D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001859:Bone Neoplasms; D020134:Catalytic Domain; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D016297:Mutagenesis, Site-Directed; D012516:Osteosarcoma; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D019098:Telomerase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms
D017931:DNA Primers; D004268:DNA-Binding Proteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D019098:Telomerase