title: Genetic and physical mapping, expression analysis and partial sequence of porcine PER1.
abstract: The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation. A polymorphic microsatellite marker (S0601) was isolated from a BAC clone shown to contain the PER1 gene. Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12. RT-PCR analysis demonstrated the expression of PER1 in all 11 tissues tested, consistent with the data from other mammalian species. Part of the PER1 gene was sequenced, homologous to exons 2-14 of the human gene and encoding the N-terminus of porcine PER1. The predicted amino acid sequence of the partial pig PER1 protein shares over 96% identity with its human orthologue.
authors.affiliation: Roslin Institute, Roslin, Midlothian, UK. tom.skinner@bbsrc.ac.uk
authors.forename: T M
authors.initials: TM
authors.lastname: Skinner
authors.forename: N L
authors.initials: NL
authors.lastname: Lopez-Corrales
authors.forename: S I
authors.initials: SI
authors.lastname: Anderson
authors.forename: A S
authors.initials: AS
authors.lastname: Loudon
authors.forename: S
authors.initials: S
authors.lastname: Lowden
authors.forename: C S
authors.initials: CS
authors.lastname: Haley
authors.forename: A L
authors.initials: AL
authors.lastname: Archibald
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D056950:Period Circadian Proteins; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*; D026801:Synteny
chemical_list: D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C539022:PER1 protein, human; D056950:Period Circadian Proteins; D012333:RNA, Messenger