title: Peritoneal closure reduces port site metastases: results of an experiment in a rat ovarian cancer model.
abstract: BACKGROUND
  The arm of this study was to assess the role of peritoneal closure in the prevention of port site metastasis after carbon dioxide (CO2) CO2 pneumoperitoneum.
  
  
  METHODS
  We developed a xenograft ovarian cancer model by intraperitoneal injection of 27 106 IGR-OV1 line cells in nude rats Seven days after the inoculation, the animals underwent a CO2 pneumoperitoneum. At the end of the procedure, port sites were randomly closed either with suture of peritoneum (n = 14, group A) or without suture of peritoneum (n = 12, group B). The rats were killed 7 days after surgery and their port site scars were resected. Tumor implantation was assessed by a pathologist who was blinded to the type of wound closure.
  
  
  RESULTS
  The animals in group B were significantly more likely to have at least one port site metastasis frequent (seven of 12, or 58.3%) than those in group A (two of 14, or (14.3%) (p = 0.037). Port sites with metastases were seen more frequently in group B (eight of 24, or (33.3%) than in group A (three of 28, or 10.7%) (p = 0.046).
  
  
  CONCLUSIONS
  Our results shows that peritoneum closure decreases the risk of port site metastasis.
authors.affiliation: Department of Obstetrics and Gynecology, La Conception University Hospital, 147 Boulevard Baille, 13005, Marseille, France. aubert.agostini@wanadoo.fr
authors.forename: A
authors.initials: A
authors.lastname: Agostini
authors.forename: F
authors.initials: F
authors.lastname: Robin
authors.forename: J P
authors.initials: JP
authors.lastname: Jais
authors.forename: M
authors.initials: M
authors.lastname: Aggerbeck
authors.forename: F
authors.initials: F
authors.lastname: Vildé
authors.forename: B
authors.initials: B
authors.lastname: Blanc
authors.forename: F
authors.initials: F
authors.lastname: Lécuru
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000627:therapeutic use; D004195:Disease Models, Animal; D005260:Female; D007327:Insufflation / Q000379:methods; D010535:Laparoscopy / Q000379:methods; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation / Q000379:methods; D010051:Ovarian Neoplasms / Q000556:secondary* / Q000601:surgery; D010537:Peritoneum / Q000601:surgery*; D051381:Rats; D011923:Rats, Nude; D014183:Transplantation, Heterologous / Q000379:methods; D014407:Tumor Cells, Cultured
chemical_list: D002245:Carbon Dioxide