Cross-linked matrix tissue sealant protects against mortality and hemorrhage in an acute renal injury model in heparinized rats.
OBJECTIVE
We evaluated the efficacy of a cross-linked matrix tissue sealant compared with oxidized cellulose and electrocautery in an acute renal injury model.


METHODS
Sprague-Dawley rats underwent partial nephrectomy after intravenous heparin anticoagulation. The cut surface received 1 of 4 therapies, namely no treatment, cross-linked matrix tissue sealant, electrocautery or Surgicel (Johnson & Johnson, New Brunswick, New Jersey) oxidized cellulose. Blood pressure was continually monitored, survival time was determined and total blood loss was quantified in each animal.


RESULTS
Animals that received no treatment, electrocautery or oxidized cellulose died within 13 minutes of injury with a decline in blood pressure to 0 mm. Hg. In cross-linked matrix treated rats hemostasis was immediate, resulting in no blood loss after cross-linked matrix application. Mean arterial pressure +/- SEM was maintained at 136 +/- 6 mm. Hg. All cross-linked matrix treated animals survived.


CONCLUSIONS
Cross-linked matrix tissue sealant provided immediate hemostasis and protection from a decline in blood pressure after renal trauma in heparinized rats. Cross-linked matrix tissue sealant may benefit patients with severe renal injuries. In the future cross-linked matrix may be useful for long-term partial nephrectomy repair.
Petratos
Peter B
PB
Center for Pediatric Urology and Laboratory for Minimally Invasive Urologic Surgery, Children's Hospital of New York, Weill Medical College of Cornell University, New York, New York, USA.
Ito
Keiichi
K
Felsen
Diane
D
Poppas
Dix
D
D000818:Animals; D002483:Cellulose, Oxidized; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004564:Electrocoagulation; D006470:Hemorrhage / Q000401:mortality / Q000473:pathology*; D006490:Hemostatics / Q000494:pharmacology*; D007668:Kidney / Q000293:injuries* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014950:Wounds, Penetrating / Q000401:mortality / Q000473:pathology*
D002483:Cellulose, Oxidized; D003432:Cross-Linking Reagents; D006490:Hemostatics; C013695:Surgicel