title: Topoisomerase II alpha as a reliable proliferation marker in meningiomas.
abstract: The value of DNA Topoisomerase IIalpha expression as a proliferation marker in meningiomas was investigated. The correlation to MIB-1 expression and tumor grade in 85 meningiomas (60 classical, 19 atypical and 6 anaplastic) was analysed by immunohistochemistry. MIB-1 labeling indices (LI) were 1.1% (+/- 0.85) for classical meningiomas, 1.9% (+/- 1.5) for atypical meningiomas and 5.6% (+/- 4.1) for anaplastic meningiomas, differences statistically significant (p = 0.03, < 0.0001, < 0.0001). Topoisomerase IIalpha LIs were 1.4% (+/- 1.2) for classical, 3.2% (+/- 2.4) for atypical and 9.7% (+/- 6.6) for anaplastic meningiomas, differences statistically significant (p = 0.003, < 0.0001, < 0.0001). Proliferation indices based on cells staining for MIB-1 and Topoisomerase IIalpha correlated highly with one another (r= 0.906, p <0.0001). Topoisomerase IIalpha exhibited a more distinct, less variable nuclear staining pattern compared to MIB-1 expression. DNA Topoisomerase IIalpha LI represents a reliable alternative to MIB-1 as a proliferation marker in human meningiomas, especially for computer assisted assessment, where a distinct uniform staining pattern is required.
authors.affiliation: Department of Neurosurgery, University of Vienna Medical School, Austria. Karl.Roessler@univie.ac.at
authors.forename: Karl
authors.initials: K
authors.lastname: Roessler
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gatterbauer
authors.forename: Klaus
authors.initials: K
authors.lastname: Kitz
mesh_terms: D000704:Analysis of Variance; D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor / Q000032:analysis*; D002455:Cell Division; D004250:DNA Topoisomerases, Type II / Q000032:analysis*; D004268:DNA-Binding Proteins; D006801:Humans; D019394:Ki-67 Antigen; D008579:Meningioma / Q000201:enzymology* / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis
chemical_list: D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D004250:DNA Topoisomerases, Type II