Effect of growth factors on ex vivo bone marrow cell expansion using three-dimensional matrix support.
To develop a culture system for bone marrow (BM) cell expansion, we examined the effect of growth factors (GFs) on the proliferation and differentiation of BM cells cultured in three-dimensional (3D) scaffolds of porous polyvinyl formal (PVF) resin. Murine BM cells were cultured for 2 weeks in the PVF resin or in culture dishes as a control, in the presence or absence of 4 GFs (erythropoietin, stem cell factor, interleukin [IL]-3, and IL-6). These GFs remarkably stimulated cell proliferation both in PVF and dish cultures. In addition, the PVF cultures showed enhanced cell proliferation in comparison with the corresponding dish cultures. Moreover, PVF cultures with GFs revealed the highest number of colony-forming units and the highest percentage of hematopoietic progenitor cells (HPCs) among all the cultures examined. Therefore, this 3D PVF culture system with GFs is considered as a potential alternative method for the ex vivo expansion of HPCs.
Tun
Thein
T
Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Miyoshi
Hirotoshi
H
Aung
Tun
T
Takahashi
Satoru
S
Shimizu
Ritsuko
R
Kuroha
Takashi
T
Yamamoto
Masayuki
M
Ohshima
Norio
N
D000818:Animals; D001854:Bone Marrow Cells; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D003470:Culture Media; D015672:Erythroid Precursor Cells; D006133:Growth Substances / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011145:Polyvinyls
D003470:Culture Media; D006133:Growth Substances; D011145:Polyvinyls