title: Crystallization and preliminary X-ray crystallographic analysis of aspartate 1-decarboxylase from Helicobacter pylori.
abstract: Aspartate 1-decarboxylase (PanD) catalyzes the alpha-decarboxylation of L-aspartate in the major route of beta-alanine production for pantothenate biosynthesis in bacteria. Pantothenate is synthesized in microorganisms, plants and fungi but not in animals and thus the enzymes of its biosynthetic pathway are potential targets for developing agents against these organisms. PanD from the pathogenic bacterium Helicobacter pylori has been overexpressed in Escherichia coli and crystallized using sodium formate as a precipitant. Crystals diffracted to better than 1.5 A Bragg spacing upon exposure to synchrotron X-rays. Diffraction data to 1.55 A have been collected from a crystal grown in the presence of the substrate analogue isoasparagine. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 81.83, c = 93.78 A. The asymmetric unit contains one subunit of PanD, with a corresponding crystal volume per protein mass (V(M)) of 2.85 A(3) Da(-1) and a solvent content of 56.8%.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Ae Ran
authors.initials: AR
authors.lastname: Kwon
authors.forename: Byung Il
authors.initials: BI
authors.lastname: Lee
authors.forename: Byung Woo
authors.initials: BW
authors.lastname: Han
authors.forename: Hyung Jun
authors.initials: HJ
authors.lastname: Ahn
authors.forename: Jin Kuk
authors.initials: JK
authors.lastname: Yang
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yoon
authors.forename: Se Won
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D005968:Glutamate Decarboxylase / Q000737:chemistry* / Q000235:genetics; D016480:Helicobacter pylori / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: C019104:aspartate-alpha-decarboxylase; D005968:Glutamate Decarboxylase