Selective binding of two-armed diketopiperazine receptors to side-chain-protected peptides.
The binding properties of two-armed receptors based on a diketopiperazine template toward side-chain-protected peptides have been elucidated. Selective binding of these diketopiperazine receptors is not limited to side-chain-free peptides, but combinatorial on-bead assays show that also side-chain-protected peptides are recognized with high specificity. Furthermore, the screening of several dye-marked diketopiperazine receptors against an encoded side-chain-protected tripeptide library demonstrated not only their high binding specificities but also revealed that small structural changes suffice to alter the binding preferences significantly.
Conza
Matteo
M
Institute of Organic Chemistry, University Basel, St. Johanns Ring 19, CH-4056 Basel, Switzerland.
Wennemers
Helma
H
D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques; D054659:Diketopiperazines; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D010879:Piperazines / Q000737:chemistry*; D011485:Protein Binding; D018000:Receptors, Peptide / Q000737:chemistry*; D013379:Substrate Specificity
D054659:Diketopiperazines; D019151:Peptide Library; D010455:Peptides; D010879:Piperazines; D018000:Receptors, Peptide