Fluorescent vital labeling to track cestodes in a copepod intermediate host.
In experimental studies of host-parasite interactions, it is often important to track parasites in their hosts and to discriminate between individual parasites. We used the fluorescent tracer dyes 7-amino-4-chloromethylcoumarin (CMAC) and, 5-chloromethylfluorescein diacetate (CMFDA) for vital labeling of Schistocephalus solidus (Cestoda) coracidia larvae. Labeling was fast and easy to perform and enabled microscopic detection of parasites appearing as procercoids in the hemocoel of the copepod intermediate host at 3 h after exposure. The label was still visible after 14 days. Extensive controls showed that CMAC (20 microM) labeling did not harm tapeworms or copepods. CMFDA (2 microM) reduced host survival, but the dye concentration can be decreased to avoid this in future studies. The new labeling method presented here has been very useful to track S. solidus parasites. It can be valuable for other parasites also and may be particularly suitable for visualization of individual live macroparasites in invertebrate hosts, for which we are not aware of any other appropriate method.
Kurtz
Joachim
J
Department of Evolutionary Ecology, Max-Planck-Institute for Limnology, August-Thienemann-Str. 2, 24306 Plön, Germany. kurtz@mpil-ploen.mpg.de
van der Veen
Ineke T
IT
Christen
Mira
M
D000818:Animals; D002589:Cestoda / Q000302:isolation & purification* / Q000502:physiology; D003374:Coumarins; D003445:Crustacea / Q000469:parasitology*; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence
C453681:7-amino-4-chloromethylcoumarin; D003374:Coumarins; D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein